Dpse\GA19622 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA19622
Entrez Gene ID	4803095
Full Name	GA19622 gene product from transcript GA19622-RB
Synonyms	GA19622,Su(var)3-9,dpse_GLEANR_2994
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001359856.4	XP_001359893.3	uncharacterized protein, isoform C
XM_003736855.2	XP_003736903.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA19622 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA19622 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe02506	XM_003736855.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA19622), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ODe02507	XM_001359856.4 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA19622), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA19622 ( XM_003736855.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_003736903.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA19622), mRNA.

Price & Availability↑

CloneID	ODe02506
Clone ID Related Accession (Same CDS sequence)	XM_003736855.2
Accession Version	XM_003736855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_003736855.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGG CTGAAGCTCA AGTCAATGTG AATCGGAATT TGCAAAAGCA GGACCTTAGG 
AATTTGGAAG TCAGCAACCT TACACCTCTG TCGCCCGAGG TGATCTCGCG CCAGGCGACC 
ATCAACATCG GCACAATCGG ACATGTAGCA CACGGAAAGT CCACCGTAGT CAAAGCCATA 
TCCGGCGTGC AGACGGTCCG CTTTAAAAAC GAATTGGAGC GCAACATTAC AATTAAGCTC 
GGTTATGCCA ATGCCAAAAT CTACAAGTGC GACAATCCCA AGTGCCCGCG CCCGGCCAGC 
TTTGTTTCGG ATGCCTCGAG CAAGGATGAC AGCTTGCCCT GCACGCGACC CGCCTGCTCT 
GGCAATTTCA GACTGGTGCG GCATGTGAGC TTTGTGGATT GCCCCGGTCA CGACATTCTT 
ATGGCCACCA TGTTGAACGG TGCCGCTGTA ATGGATGCCG CACTGCTTCT CATTGCTGGC 
AATGAATCCT GCCCTCAACC GCAAACCTCT GAGCATTTGG CTGCCATTGA GATCATGAAA 
CTGAAACAGA TCTTGATATT GCAAAACAAA ATCGATCTGA TCAAGGAGAG CCAGGCGAAG 
GAGCAGTACG AGGAGATCAC CAAGTTTGTC CAGGGCACCG TTGCCGAGGG CGCGCCGATT 
ATACCCATTT CGGCCCAGCT CAAATACAAC ATTGATGTGC TCTGTGAATA TATTGTGAAC 
AAGATTCCTG TACCGCAGCG AGACTTTAAT GCGCCGCCCC GTCTGATTGT CATCCGCTCC 
TTCGATGTGA ACAAGCCGGG CTGTGAGGTG CAGGACCTGA AGGGCGGCGT CGCCGGTGGC 
TCCATTCTGA GCGGTGTGCT GAAGGTGGGC CAGGAGATCG AAGTGCGTCC CGGTGTCGTG 
ACCAAGGACA GCGATGGCAA CATTACCTGC CGCCCCATAT TCTCGCGGAT CGTTTCACTG 
TTTGCCGAAC AGAACGAACT GCAGTATGCC GTTCCTGGTG GCCTGATCGG TGTGGGCACC 
AAAATCGATC CCACGCTCTG TCGTGCCGAT CGTTTGGTGG GCCAGGTGCT GGGTGCCGTC 
GGACAGCTGC CGGACATCTA TCAGGAGCTG GAAATTTCAT ACTACCTGTT GCGTCGCCTG 
CTCGGCGTAC GTACGGATGG CGACAAGAAG GGTGCGCGTG TCGAGAAGCT GCAGAAGAAC 
GAGATTCTGC TGGTCAACAT TGGATCGCTC AGCACTGGCG GCCGCATCTC GGCCACCAAG 
GGTGATTTGG CCAAGATTGT GCTCACAACT CCGGTGTGTA CCGAGAAGGG CGAGAAGATT 
GCCCTCAGCA GACGTGTGGA GAACCATTGG CGTTTGATTG GATGGGGTCA GATCTTTGGA 
GGGAAGACAA TTAATCCCGT TCTGGACAGT CAGCCGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003736903.1
CDS	98..1519
Translation	MATAEAQVNVNRNLQKQDLRNLEVSNLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFKNELERNITIKLGYANAKIYKCDNPKCPRPASFVSDASSKDDSLPCTRPACSGNFRLVRHVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKQILILQNKIDLIKESQAKEQYEEITKFVQGTVAEGAPIIPISAQLKYNIDVLCEYIVNKIPVPQRDFNAPPRLIVIRSFDVNKPGCEVQDLKGGVAGGSILSGVLKVGQEIEVRPGVVTKDSDGNITCRPIFSRIVSLFAEQNELQYAVPGGLIGVGTKIDPTLCRADRLVGQVLGAVGQLPDIYQELEISYYLLRRLLGVRTDGDKKGARVEKLQKNEILLVNIGSLSTGGRISATKGDLAKIVLTTPVCTEKGEKIALSRRVENHWRLIGWGQIFGGKTINPVLDSQPK

Target ORF information:

RefSeq Version	XM_003736855.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA19622), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003736855.2

ORF Insert Sequence:

ATGGCCACGG CTGAAGCTCA AGTCAATGTG AATCGGAATT TGCAAAAGCA GGACCTTAGG 
AATTTGGAAG TCAGCAACCT TACACCTCTG TCGCCCGAGG TGATCTCGCG CCAGGCGACC 
ATCAACATCG GCACAATCGG ACATGTAGCA CACGGAAAGT CCACCGTAGT CAAAGCCATA 
TCCGGCGTGC AGACGGTCCG CTTTAAAAAC GAATTGGAGC GCAACATTAC AATTAAGCTC 
GGTTATGCCA ATGCCAAAAT CTACAAGTGC GACAATCCCA AGTGCCCGCG CCCGGCCAGC 
TTTGTTTCGG ATGCCTCGAG CAAGGATGAC AGCTTGCCCT GCACGCGACC CGCCTGCTCT 
GGCAATTTCA GACTGGTGCG GCATGTGAGC TTTGTGGATT GCCCCGGTCA CGACATTCTT 
ATGGCCACCA TGTTGAACGG TGCCGCTGTA ATGGATGCCG CACTGCTTCT CATTGCTGGC 
AATGAATCCT GCCCTCAACC GCAAACCTCT GAGCATTTGG CTGCCATTGA GATCATGAAA 
CTGAAACAGA TCTTGATATT GCAAAACAAA ATCGATCTGA TCAAGGAGAG CCAGGCGAAG 
GAGCAGTACG AGGAGATCAC CAAGTTTGTC CAGGGCACCG TTGCCGAGGG CGCGCCGATT 
ATACCCATTT CGGCCCAGCT CAAATACAAC ATTGATGTGC TCTGTGAATA TATTGTGAAC 
AAGATTCCTG TACCGCAGCG AGACTTTAAT GCGCCGCCCC GTCTGATTGT CATCCGCTCC 
TTCGATGTGA ACAAGCCGGG CTGTGAGGTG CAGGACCTGA AGGGCGGCGT CGCCGGTGGC 
TCCATTCTGA GCGGTGTGCT GAAGGTGGGC CAGGAGATCG AAGTGCGTCC CGGTGTCGTG 
ACCAAGGACA GCGATGGCAA CATTACCTGC CGCCCCATAT TCTCGCGGAT CGTTTCACTG 
TTTGCCGAAC AGAACGAACT GCAGTATGCC GTTCCTGGTG GCCTGATCGG TGTGGGCACC 
AAAATCGATC CCACGCTCTG TCGTGCCGAT CGTTTGGTGG GCCAGGTGCT GGGTGCCGTC 
GGACAGCTGC CGGACATCTA TCAGGAGCTG GAAATTTCAT ACTACCTGTT GCGTCGCCTG 
CTCGGCGTAC GTACGGATGG CGACAAGAAG GGTGCGCGTG TCGAGAAGCT GCAGAAGAAC 
GAGATTCTGC TGGTCAACAT TGGATCGCTC AGCACTGGCG GCCGCATCTC GGCCACCAAG 
GGTGATTTGG CCAAGATTGT GCTCACAACT CCGGTGTGTA CCGAGAAGGG CGAGAAGATT 
GCCCTCAGCA GACGTGTGGA GAACCATTGG CGTTTGATTG GATGGGGTCA GATCTTTGGA 
GGGAAGACAA TTAATCCCGT TCTGGACAGT CAGCCGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA19622 ( XM_001359856.4 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001359893.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA19622), mRNA.

Price & Availability↑

CloneID	ODe02507
Clone ID Related Accession (Same CDS sequence)	XM_001359856.4
Accession Version	XM_001359856.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_001359856.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACGG CTGAAGCTCA AGTCAATGTG AATCGGAATT TGCAAAAGCA GGACCTTAGG 
AATTTGGAAG TCAGCAACCT TACACCTCTG TCGCCCGAGG TGATCTCGCG CCAGGCGACC 
ATCAACATCG GCACAATCGG ACATGTAGCA CACGGAAAGT CCACCGTAGT CAAAGCCATA 
TCCGGCGTGC AGACGGTCCG CTTTAAAAAC GAATTGGAGC GCAACATTAC AATTAAGCTC 
GAACGCTTAA GCGAAAAGAA AATCAAGCTA CTTTTGAAAT CCAAACACCA GCGACACAAA 
TACGATATCC AGCAGCAAAA GCTGCTACGG ATTCTCGCCG AGCGGCGAAA GGCCAGGGCA 
ATGACACCCC TTGCCCCCGC TTCAGTGCTA GCCCCATCCA TGGAAACAGC TCGTCCGCGT 
CTTCGCAGCA CCAGCCTAAA CTCATTAAGT CCCAGCAACA GCAGCGGCTA TGGCAGCATC 
CTCGGCTGCG ATGATAGCGA TCAATCATCA CAGCTGGTCC TGAAACCGAA TGTTCTTAAG 
CGACGACGTA GCAACTGTGT CCAGATACCC ACTCCAGCAG CCAAAAGGAG CAGGAAAAAC 
GATGGAACGA CTGTGAAGAG GCGACCCAAG GGCGAGTACA TTGTGGAGAA AATCGAGAGC 
GTGGAGGTGG TCCAGTTTCA GCCCGTTTTC TTTGTGAAAT GGCTGGGATA CGATGTCAGC 
GCGAACACAT GGGAATCGTA CGTAAATCTA TCCGATTGTG CCGAAATGGA AAAATTTGTT 
GAGCGTCATC TACAGCTGCA TCAGCATTAT ATAGCCCAGA TCACGGGTGA ACTGGATACG 
CAGTTGTCGG ACATACCGCA GACGGAAGAT CTTAAGACTA TTAGCATTGC AGAGATTGAT 
GCCTATGATC CGCTCGAGTT GCAGATTGAT TTTATACTGC TAGCGCAATA CCGTGCCGCT 
GCCAGCCGCA GCCAGCGGGA ACCCGAGCGA ATCGGTGCGC GGGCCCTGCA CCGCATGCAG 
GTCAGGCGGT CTCACTTTGC ACGGCGCAAG CAGCTGATTG ATTTATTGCT GTTTGAGCAC 
CGAATGAATC GAGTCGAGCT GCCGTCGCCG CCCATACGAG TGGAGAACAA TTGGGATCTG 
GACACGATTG ACAGCGGCTT CAAGTACATA CAAAAGAACA TAATTGGAGA AGGGGTACCC 
AAGCCGCAAG CTGGACTTGT GGGTTGTATG TGCCGCCACC AGAGCGGAGA ACAATGCACC 
GCCTCCTCCA TGTGTTGTGG AAGAATGGCT GGTGAAATCT TTGCCTACGA TCGTACCACG 
GGACGTTTGC GGCTCCGGCC GGGCAGTGCC ATCTATGAGT GCAACAGTCG ATGCTCCTGC 
GACGAGAGTT GCACGAATCG TGTGGTACAG AATGGACGAA AGCATCCGCT TGTGCTCTTT 
AAAACCAGCA ATGGCAGTGG TTGGGGTGTC CGCACACCGC AGCCATTGAA GAAGGGAGTG 
TTTGTCTGCG AATATATCGG CGAGATCATT ACGTGCGAGG AGGCTAATGA GCGTGGCAAG 
GCCTATGACG ATAATGGGCG TACCTATCTG TTTGATCTGG ACTACAATAC ATCGCGGGAT 
AGCGAGTATA CGGTGGACGC GGCCAACTTT GGCAATATAT CGCATTTTAT CAATCATTCG 
TGTGATCCCA ATCTGGCCGT GTTCCCATGC TGGATCGAGC ACTTAAATAC GGCCCTGCCA 
CATCTGGTCT TCTTTACCAT TCGCCCGATT AAGGCGGGGG AGGAGCTCAG TTTTGACTAC 
ATTCGGGCGG ACAACGAGGA GGTGCCATAT GAGAATCTAT CGACGGCTGC GCGTGTCCAG 
TGTCGGTGTG GGGCGGCCAA TTGCCGGAAG GTCCTATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001359893.3
CDS	119..2020
Translation	MATAEAQVNVNRNLQKQDLRNLEVSNLTPLSPEVISRQATINIGTIGHVAHGKSTVVKAISGVQTVRFKNELERNITIKLERLSEKKIKLLLKSKHQRHKYDIQQQKLLRILAERRKARAMTPLAPASVLAPSMETARPRLRSTSLNSLSPSNSSGYGSILGCDDSDQSSQLVLKPNVLKRRRSNCVQIPTPAAKRSRKNDGTTVKRRPKGEYIVEKIESVEVVQFQPVFFVKWLGYDVSANTWESYVNLSDCAEMEKFVERHLQLHQHYIAQITGELDTQLSDIPQTEDLKTISIAEIDAYDPLELQIDFILLAQYRAAASRSQREPERIGARALHRMQVRRSHFARRKQLIDLLLFEHRMNRVELPSPPIRVENNWDLDTIDSGFKYIQKNIIGEGVPKPQAGLVGCMCRHQSGEQCTASSMCCGRMAGEIFAYDRTTGRLRLRPGSAIYECNSRCSCDESCTNRVVQNGRKHPLVLFKTSNGSGWGVRTPQPLKKGVFVCEYIGEIITCEEANERGKAYDDNGRTYLFDLDYNTSRDSEYTVDAANFGNISHFINHSCDPNLAVFPCWIEHLNTALPHLVFFTIRPIKAGEELSFDYIRADNEEVPYENLSTAARVQCRCGAANCRKVLF

Target ORF information:

RefSeq Version	XM_001359856.4
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant C (Dpse\GA19622), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001359856.4

ORF Insert Sequence:

ATGGCCACGG CTGAAGCTCA AGTCAATGTG AATCGGAATT TGCAAAAGCA GGACCTTAGG 
AATTTGGAAG TCAGCAACCT TACACCTCTG TCGCCCGAGG TGATCTCGCG CCAGGCGACC 
ATCAACATCG GCACAATCGG ACATGTAGCA CACGGAAAGT CCACCGTAGT CAAAGCCATA 
TCCGGCGTGC AGACGGTCCG CTTTAAAAAC GAATTGGAGC GCAACATTAC AATTAAGCTC 
GAACGCTTAA GCGAAAAGAA AATCAAGCTA CTTTTGAAAT CCAAACACCA GCGACACAAA 
TACGATATCC AGCAGCAAAA GCTGCTACGG ATTCTCGCCG AGCGGCGAAA GGCCAGGGCA 
ATGACACCCC TTGCCCCCGC TTCAGTGCTA GCCCCATCCA TGGAAACAGC TCGTCCGCGT 
CTTCGCAGCA CCAGCCTAAA CTCATTAAGT CCCAGCAACA GCAGCGGCTA TGGCAGCATC 
CTCGGCTGCG ATGATAGCGA TCAATCATCA CAGCTGGTCC TGAAACCGAA TGTTCTTAAG 
CGACGACGTA GCAACTGTGT CCAGATACCC ACTCCAGCAG CCAAAAGGAG CAGGAAAAAC 
GATGGAACGA CTGTGAAGAG GCGACCCAAG GGCGAGTACA TTGTGGAGAA AATCGAGAGC 
GTGGAGGTGG TCCAGTTTCA GCCCGTTTTC TTTGTGAAAT GGCTGGGATA CGATGTCAGC 
GCGAACACAT GGGAATCGTA CGTAAATCTA TCCGATTGTG CCGAAATGGA AAAATTTGTT 
GAGCGTCATC TACAGCTGCA TCAGCATTAT ATAGCCCAGA TCACGGGTGA ACTGGATACG 
CAGTTGTCGG ACATACCGCA GACGGAAGAT CTTAAGACTA TTAGCATTGC AGAGATTGAT 
GCCTATGATC CGCTCGAGTT GCAGATTGAT TTTATACTGC TAGCGCAATA CCGTGCCGCT 
GCCAGCCGCA GCCAGCGGGA ACCCGAGCGA ATCGGTGCGC GGGCCCTGCA CCGCATGCAG 
GTCAGGCGGT CTCACTTTGC ACGGCGCAAG CAGCTGATTG ATTTATTGCT GTTTGAGCAC 
CGAATGAATC GAGTCGAGCT GCCGTCGCCG CCCATACGAG TGGAGAACAA TTGGGATCTG 
GACACGATTG ACAGCGGCTT CAAGTACATA CAAAAGAACA TAATTGGAGA AGGGGTACCC 
AAGCCGCAAG CTGGACTTGT GGGTTGTATG TGCCGCCACC AGAGCGGAGA ACAATGCACC 
GCCTCCTCCA TGTGTTGTGG AAGAATGGCT GGTGAAATCT TTGCCTACGA TCGTACCACG 
GGACGTTTGC GGCTCCGGCC GGGCAGTGCC ATCTATGAGT GCAACAGTCG ATGCTCCTGC 
GACGAGAGTT GCACGAATCG TGTGGTACAG AATGGACGAA AGCATCCGCT TGTGCTCTTT 
AAAACCAGCA ATGGCAGTGG TTGGGGTGTC CGCACACCGC AGCCATTGAA GAAGGGAGTG 
TTTGTCTGCG AATATATCGG CGAGATCATT ACGTGCGAGG AGGCTAATGA GCGTGGCAAG 
GCCTATGACG ATAATGGGCG TACCTATCTG TTTGATCTGG ACTACAATAC ATCGCGGGAT 
AGCGAGTATA CGGTGGACGC GGCCAACTTT GGCAATATAT CGCATTTTAT CAATCATTCG 
TGTGATCCCA ATCTGGCCGT GTTCCCATGC TGGATCGAGC ACTTAAATAC GGCCCTGCCA 
CATCTGGTCT TCTTTACCAT TCGCCCGATT AAGGCGGGGG AGGAGCTCAG TTTTGACTAC 
ATTCGGGCGG ACAACGAGGA GGTGCCATAT GAGAATCTAT CGACGGCTGC GCGTGTCCAG 
TGTCGGTGTG GGGCGGCCAA TTGCCGGAAG GTCCTATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA19622 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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