LOC6554743 cDNA ORF clone, Drosophila erecta

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC6554743
Entrez Gene ID	6554743
Full Name	GG11806 gene product from transcript GG11806-RA
Synonyms	GG11806,dere_GLEANR_11869
Gene Type	protein-coding
Organism	Drosophila erecta

mRNA and Protein(s)

mRNA	Protein	Name
XM_001981050.2	XP_001981086.1	uncharacterized protein
XM_001981050.3	XP_001981086.1	eIF-2-alpha kinase GCN2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC6554743 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC6554743 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODi10497	XM_001981050.3 Latest version!	Drosophila erecta eIF-2-alpha kinase GCN2 (LOC6554743), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
ODi10497	XM_001981050.2	Drosophila erecta uncharacterized protein (Dere\GG11806), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC6554743 ( XM_001981050.2 ) cDNA ORF clone, Drosophila erecta -> XP_001981086.1 Drosophila erecta uncharacterized protein (Dere\GG11806), mRNA.

Price & Availability↑

CloneID	ODi10497
Clone ID Related Accession (Same CDS sequence)	XM_001981050.2 , XM_001981050.3
Accession Version	XM_001981050.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956553). On Dec 21, 2015 this sequence version replaced XM_001981050.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGATG AAAAGGCTAA GGAATCCTTC CGCGAGCGGC AAGCGCAGGA GTTGGAGGTT 
ATAAAGTCCA TCTTTGGCGG TGATGTGGAG GACCTGCGAC CCCAGGCGAA TCCTTCCCTG 
TGGAAGCCAA CGGACATAAG GATACAGCTT ACCCCACTTA GGGACTCCTC CAATGGACTG 
GAAACTTATG TGTGCACCAA ACTGCATGTC ACCTGTCCTT CAAAGTATCC AAAATTGCCC 
CCTAAGATAT GTCTCGAGGA GTCCAAGGGC ATGTCGGATC AGTTGTTGGA GGCCCTACGC 
AACCAACTGC AGGCTCAATC CCAAGAGCTG CGTGGGGAAG TGATGATTTA TGAACTGGCC 
CAGACCGTTC AGGCCTTTCT CCTCGAACAC AACAAACCGC CAAAGGGCTC TTTCTACGAT 
CAGATGCTAC AAGACAAACA GAAGCGCGAC CAGGAGCTAC AGGACATGCA GCGGCAGCGG 
GAGTCTGTGC AACGCCAAAC ACTCATCGAC GAGGTGGAGC GGCGCAAAGA GATGTTTAAA 
ACAGAGGCTA AAAGGCGAGG CGAACCAAGA CGCAGCATGA GCGAGTCTAA CCCACGGCAT 
CCTAGCTCGT CGGAGAGCTC CGAGAACTCC TCGCCCTACT ATCGTGGTCA CATCTACCCC 
TCCAAATGCC TCGATCACCG CAACACGGAG ACGCTCTACT TCCACAAGAT GGGCAGGCAA 
ATTCAACGAG GATGCTGCGT TGGTCATTCC CAACGAGGGT GCATCGCCTA CACTGGCATA 
GATATGCACT GCGGCCAGTT GCTCTATATC ACAGAATGGA ACATCAAGTA CAGCCAACTG 
GAGCAGCCGT GCATCGGCGG AGGCAAGTGC CACTGGAGCA GCGAGTCAAA ATGCATGGGC 
AGTCATCGTG TGGACGAAGT GATCGCCTCC ATTGAAAAGC AGGTGTCCTC GCTGGCCCAG 
CTGCAGCACA AAAATCTCGT CTCGTACGAG TGCGTTCTTT GCATCAAGCG GAAGGAAGGC 
TTACTTGTGT ATTTGGTCCA GGACTTCCTC CTCGGCACCA GCGTGTTCAG CATATCCTCC 
TCGCTGGGTT GGTGCATGGA CGGCGCACGA ATGGTTGCAC GCGGCGTGCT GGATGCCTTA 
ATATTTCTTC ACAACAAGGG TGTATCCCAC AGTCACTTGC TCGACAGCAC TGTTTTTATG 
GACAACACAG GCAATGTTCG TGTATCAGAC TTTTCGCTAG TTCCCAATCT TTTAGAACTT 
CTGAGCGGAG CTGGCCAAAG CAGCAGTTGC GGGGACTTAC CCGCGTTAGG TGCCCTGGTG 
GAGAGCTTGA TGCCCACTAA CAGTTATGAG ATGCGCGACT TTGTGGACAA ATGCAACTCG 
GACCGCACGC TTTCCGCATC AGAGTTGTTG GAGCATCCAT TCTTACGCTT TTACGTAGAC 
AATGGCCAGC AGCAAGTGAT GCCATTAACA CAGCAGCAGC ATCCGAATAC CGTCCAGCGC 
ACTGGACCCG CCATGCCCTA TCAGATACCG ACACTGGCCT TAAGCCAATC CCGCCTGCGA 
ACAGAATTCG AGGTGCTCAT GTACTTGGGA AAGGGTGCCT ATGGTGACGT GCTAAAAGTG 
CGCAACATTT TGGACAACCG AGAGTACGCC ATCAAGCGCA TACCGTTGCC CGCGAGGAGT 
CGCCAGTTGT ACAAAAAGAT GACGCGCGAG GTGGAGCTGC TTTCGCGGCT GAATCATGAG 
AACGTTGTTC GATACTTTAA CAGTTGGATA GAGAGTGTGG ATGACGCAGA TGCCGCTGAG 
ATGGATAAAT TGCTGGGTGG TGAGTGGTCC CAGAGTCAGC AGGACCTTAG CGTGAAACCT 
GCCAAGTCAC CACAGCTGGG GCCCACTTTA GAGGAGGATG AGGATGAAGA GGACAGCTCA 
TCCAGCATGT GGAACGGTTA CATACCCAAC ATGGAGGACT CCGACTCGGA TGGCATTGAA 
TTTGTGGACT CCAACGGCAA AGTGGCTGTC TACGACGACG AAGAACAAGA GGAATCCACC 
AGAGGCAGGA CTAATTCGCC CAGACCTTTG ATGCAGGTCA TGTACATCCA GATGGAGTTC 
TGCGAGAAAT GCACACTTCG CACCGCAATC GATGACAATC TTTTCAATGA CACGGATCGT 
CTGTGGCGTT TATTTCGGGA GATTGCCGAG GGCCTCGCGC ATATTCACCA GCAAGGCATT 
ATTCATCGGG ACCTAAAGCC CGTTAACATT TTCTTGGACT CGCACGATCA GATCAAGATC 
GGTGACTTTG GTCTGGCCAC CACCAGTTTT TTGGCCCTGC AGGCCCACGA CACAGCACCT 
GCGCCTGTTA ACCAGATAAC CAGTGCGGAG GACGGTACTG GCACTGGTAA GGTGGGCACC 
ACTCTTTACG TGGCCCCCGA GTTGACAGGA AATGCTTCCA AGTCTGTGTA CAACCAGAAG 
GTTGATATGT ACACCCTGGG AGTCATTCTC TTCGAGATGT GCCAACCGCC ATTCGACACA 
AGCATGGAGC GCGCTCAGAC CATTATGGCC CTCAGGAATG TTTCCATCAA TATACCGGAT 
GCCATGCTCA AGGATCCGAA GTATGAGAAG ACGGTGAAGA TGCTTCAGTG GCTTCTTAAC 
CACGATCCAG CCCAGAGACC TACGGCCGAG GAGCTGCTCA TCTCAGACCT GGTTCCACCA 
GCCCAGTTGG AGGCCAATGA GCTGCAAGAA ATGCTGCGTC ACGCCCTCGC CAATCCGCAG 
AGCAAGGCCT ACAAGAACCT GGTTGCCCGG TGTCTTCAGC AGGAGAGCGA CGAGGTGCTG 
GAACACACCT ACCACTTGGG CAGCAGTCGA GCTATGAAGT CCTGGAACTC GGCAATCATA 
ATCGATGATA TAGTGTCCCT GAATCCCGTG ATTGAGTTTG TTAAGGCCAA GGTCGTGAAT 
CTATTCCGCA AGCACGGAGC TATAGAGGTG GACTCACCGC TCCTGTCCCC ACTTTCCGCA 
AGAAACAGCA GTGCGAATGC GAACGCCAAT GCCGTGCACC TGATGACGCA CTCGGGATGT 
GTGGTGGTGC TACCCTGTGA TCTGCGGACA CAGTTTGCCC GCCACGTGAC CATGAACAGC 
GTGAATCTCA TCCGGCGCTA TTGTGTGGAC CGTGTATATC GGGAGGAGCG AGTGTTCAAT 
TTCCATCCCA AACAAAGCTA CGACTGCTCT TTCGATATTA TTGCGCCCAC AACTGGAAGC 
CATCTAGTGG ATGCGGAGCT GCTATCGTTG GCTTTTGAAA TCACCAGTGA ACTGCCCCGT 
TTACGGGAAA AGAATCTGGC GATCCGCATG AATCACACTA ATCTGTTGAG GGCCATCCTT 
ATCTTCTGCA ATGTTCCAAA GGCTCAGTAT GAAGCACTTT TCGAGGGCAC CATGGACTTT 
ATCGAGTCGC GCATCTCACG CTTCCAATTT CACTCGAGTA TAACAGGGAT TATGGAAAAG 
TCGCGGACAT CTGCTCAAAC TCTAATGGAC ATGTTGTTAG CCAACTTTCT GTTGACTGGT 
TCCAGAAGCA CGGTGGACGA TTCCGCGCTG AAATCGTTGA TGCGGGGAAA GGGCGAAGCG 
GCTTCACTGG CAAGAGGAGC ACTACGGGAA CTTGAAACAG TTGTGGGACT GGCTTACAGT 
CTAGGCGTAA AATGTCCTAT TCACATATGG GCTGGCTTGC CCATCAGTTT CGATAGTGCC 
AGCAATGGTG GAATTGTGTG GCAGATGACC GCTGACCTAA AGCCGAATCG CTCTGGTCAT 
CCTTCAGTTC TGGCGATCGG GCAGAGATAC GATTCCATGC TGCATGAATT CCAGAAGCAG 
GCGCAGAAGT TTAATCCAGC CTTGCCGGCA CGAGGAGTTC TGAGCGGAGC GGGTTTGACG 
TTTTCCCTAG ACAAACTAGT AGCTGCTGTG GGTGTGGAAT ACGCTAAGGA TTGTCGGGCG 
ATAGACGTGG GCATTTGCGT GTGTGGAACG CGTCCGCCGC TGAAGGATGT GACCTACATC 
ATGCGCCTGC TGTGGTCGGT TGGCATTCGT TGTGGCATTG TGGAGGCCGC CAGCGAATTG 
GGTGATGAAG CACAGGATCT TGCTCGCCTT GGGGCGTTAC ACGTCATCCT AGTGGCGGAA 
AATGGATCCT TGAGAGTGCG CTCTTTCGAG AGGGACCGCT TTCAGGAGCG TCACCTAACA 
AGAGCCGAAC TGGTGGAGTT CATCCAGAAG ATGCTGCGAA CTGATGGACT CAATGGAACC 
ACCGTCGACA ACTTCAGCCA GTTGTCAACC ATGGGTAGTG GCGACAGCAG AAGCAGTGGT 
GGCAAGGAAC GGGAACGCGG CGAGAATGGT CTGAGCACCT CCGCATCGAA TGCCACCATT 
AAGAACAGCT ATAGCCAGCT GCCGAATCTC CAGGTGACGT TTCTCACCCA CGACAAGCCT 
ACGGCGAACT ACAAAAGACG CCTGGAGAAC CAGGTGGCGC AGCAGATGAG CTCAACGCTA 
TCGCAGTTCC TCAAGAAAGA GACCTTTGTG GTCCTGGTGG TGGAGTTGCC GCCTGCTGTT 
GTAAATGCCA TTGTAGGGGC CATCAATCCA CGAGAAGTAC GCAAGAGAGA GACTGAACCG 
GAAATCAACT TTGTGATCGA GCGATTTTCG AAATATAAGC GCTATATATC CGAGATAAAC 
GAAGAGATTG TGGACTATCT GAGCGATGCC AAGACTCCCA TTGTGGCCTT GTACAGCATT 
TCCGATTCCT ACTACCGCGT TATTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001981086.1
CDS	343..5112
Translation	MADEKAKESFRERQAQELEVIKSIFGGDVEDLRPQANPSLWKPTDIRIQLTPLRDSSNGLETYVCTKLHVTCPSKYPKLPPKICLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKPPKGSFYDQMLQDKQKRDQELQDMQRQRESVQRQTLIDEVERRKEMFKTEAKRRGEPRRSMSESNPRHPSSSESSENSSPYYRGHIYPSKCLDHRNTETLYFHKMGRQIQRGCCVGHSQRGCIAYTGIDMHCGQLLYITEWNIKYSQLEQPCIGGGKCHWSSESKCMGSHRVDEVIASIEKQVSSLAQLQHKNLVSYECVLCIKRKEGLLVYLVQDFLLGTSVFSISSSLGWCMDGARMVARGVLDALIFLHNKGVSHSHLLDSTVFMDNTGNVRVSDFSLVPNLLELLSGAGQSSSCGDLPALGALVESLMPTNSYEMRDFVDKCNSDRTLSASELLEHPFLRFYVDNGQQQVMPLTQQQHPNTVQRTGPAMPYQIPTLALSQSRLRTEFEVLMYLGKGAYGDVLKVRNILDNREYAIKRIPLPARSRQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDADAAEMDKLLGGEWSQSQQDLSVKPAKSPQLGPTLEEDEDEEDSSSSMWNGYIPNMEDSDSDGIEFVDSNGKVAVYDDEEQEESTRGRTNSPRPLMQVMYIQMEFCEKCTLRTAIDDNLFNDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDFGLATTSFLALQAHDTAPAPVNQITSAEDGTGTGKVGTTLYVAPELTGNASKSVYNQKVDMYTLGVILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKTVKMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALANPQSKAYKNLVARCLQQESDEVLEHTYHLGSSRAMKSWNSAIIIDDIVSLNPVIEFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSSANANANAVHLMTHSGCVVVLPCDLRTQFARHVTMNSVNLIRRYCVDRVYREERVFNFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFEITSELPRLREKNLAIRMNHTNLLRAILIFCNVPKAQYEALFEGTMDFIESRISRFQFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDDSALKSLMRGKGEAASLARGALRELETVVGLAYSLGVKCPIHIWAGLPISFDSASNGGIVWQMTADLKPNRSGHPSVLAIGQRYDSMLHEFQKQAQKFNPALPARGVLSGAGLTFSLDKLVAAVGVEYAKDCRAIDVGICVCGTRPPLKDVTYIMRLLWSVGIRCGIVEAASELGDEAQDLARLGALHVILVAENGSLRVRSFERDRFQERHLTRAELVEFIQKMLRTDGLNGTTVDNFSQLSTMGSGDSRSSGGKERERGENGLSTSASNATIKNSYSQLPNLQVTFLTHDKPTANYKRRLENQVAQQMSSTLSQFLKKETFVVLVVELPPAVVNAIVGAINPREVRKRETEPEINFVIERFSKYKRYISEINEEIVDYLSDAKTPIVALYSISDSYYRVII

Target ORF information:

RefSeq Version	XM_001981050.2
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein (Dere\GG11806), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001981050.2

ORF Insert Sequence:

ATGGCGGATG AAAAGGCTAA GGAATCCTTC CGCGAGCGGC AAGCGCAGGA GTTGGAGGTT 
ATAAAGTCCA TCTTTGGCGG TGATGTGGAG GACCTGCGAC CCCAGGCGAA TCCTTCCCTG 
TGGAAGCCAA CGGACATAAG GATACAGCTT ACCCCACTTA GGGACTCCTC CAATGGACTG 
GAAACTTATG TGTGCACCAA ACTGCATGTC ACCTGTCCTT CAAAGTATCC AAAATTGCCC 
CCTAAGATAT GTCTCGAGGA GTCCAAGGGC ATGTCGGATC AGTTGTTGGA GGCCCTACGC 
AACCAACTGC AGGCTCAATC CCAAGAGCTG CGTGGGGAAG TGATGATTTA TGAACTGGCC 
CAGACCGTTC AGGCCTTTCT CCTCGAACAC AACAAACCGC CAAAGGGCTC TTTCTACGAT 
CAGATGCTAC AAGACAAACA GAAGCGCGAC CAGGAGCTAC AGGACATGCA GCGGCAGCGG 
GAGTCTGTGC AACGCCAAAC ACTCATCGAC GAGGTGGAGC GGCGCAAAGA GATGTTTAAA 
ACAGAGGCTA AAAGGCGAGG CGAACCAAGA CGCAGCATGA GCGAGTCTAA CCCACGGCAT 
CCTAGCTCGT CGGAGAGCTC CGAGAACTCC TCGCCCTACT ATCGTGGTCA CATCTACCCC 
TCCAAATGCC TCGATCACCG CAACACGGAG ACGCTCTACT TCCACAAGAT GGGCAGGCAA 
ATTCAACGAG GATGCTGCGT TGGTCATTCC CAACGAGGGT GCATCGCCTA CACTGGCATA 
GATATGCACT GCGGCCAGTT GCTCTATATC ACAGAATGGA ACATCAAGTA CAGCCAACTG 
GAGCAGCCGT GCATCGGCGG AGGCAAGTGC CACTGGAGCA GCGAGTCAAA ATGCATGGGC 
AGTCATCGTG TGGACGAAGT GATCGCCTCC ATTGAAAAGC AGGTGTCCTC GCTGGCCCAG 
CTGCAGCACA AAAATCTCGT CTCGTACGAG TGCGTTCTTT GCATCAAGCG GAAGGAAGGC 
TTACTTGTGT ATTTGGTCCA GGACTTCCTC CTCGGCACCA GCGTGTTCAG CATATCCTCC 
TCGCTGGGTT GGTGCATGGA CGGCGCACGA ATGGTTGCAC GCGGCGTGCT GGATGCCTTA 
ATATTTCTTC ACAACAAGGG TGTATCCCAC AGTCACTTGC TCGACAGCAC TGTTTTTATG 
GACAACACAG GCAATGTTCG TGTATCAGAC TTTTCGCTAG TTCCCAATCT TTTAGAACTT 
CTGAGCGGAG CTGGCCAAAG CAGCAGTTGC GGGGACTTAC CCGCGTTAGG TGCCCTGGTG 
GAGAGCTTGA TGCCCACTAA CAGTTATGAG ATGCGCGACT TTGTGGACAA ATGCAACTCG 
GACCGCACGC TTTCCGCATC AGAGTTGTTG GAGCATCCAT TCTTACGCTT TTACGTAGAC 
AATGGCCAGC AGCAAGTGAT GCCATTAACA CAGCAGCAGC ATCCGAATAC CGTCCAGCGC 
ACTGGACCCG CCATGCCCTA TCAGATACCG ACACTGGCCT TAAGCCAATC CCGCCTGCGA 
ACAGAATTCG AGGTGCTCAT GTACTTGGGA AAGGGTGCCT ATGGTGACGT GCTAAAAGTG 
CGCAACATTT TGGACAACCG AGAGTACGCC ATCAAGCGCA TACCGTTGCC CGCGAGGAGT 
CGCCAGTTGT ACAAAAAGAT GACGCGCGAG GTGGAGCTGC TTTCGCGGCT GAATCATGAG 
AACGTTGTTC GATACTTTAA CAGTTGGATA GAGAGTGTGG ATGACGCAGA TGCCGCTGAG 
ATGGATAAAT TGCTGGGTGG TGAGTGGTCC CAGAGTCAGC AGGACCTTAG CGTGAAACCT 
GCCAAGTCAC CACAGCTGGG GCCCACTTTA GAGGAGGATG AGGATGAAGA GGACAGCTCA 
TCCAGCATGT GGAACGGTTA CATACCCAAC ATGGAGGACT CCGACTCGGA TGGCATTGAA 
TTTGTGGACT CCAACGGCAA AGTGGCTGTC TACGACGACG AAGAACAAGA GGAATCCACC 
AGAGGCAGGA CTAATTCGCC CAGACCTTTG ATGCAGGTCA TGTACATCCA GATGGAGTTC 
TGCGAGAAAT GCACACTTCG CACCGCAATC GATGACAATC TTTTCAATGA CACGGATCGT 
CTGTGGCGTT TATTTCGGGA GATTGCCGAG GGCCTCGCGC ATATTCACCA GCAAGGCATT 
ATTCATCGGG ACCTAAAGCC CGTTAACATT TTCTTGGACT CGCACGATCA GATCAAGATC 
GGTGACTTTG GTCTGGCCAC CACCAGTTTT TTGGCCCTGC AGGCCCACGA CACAGCACCT 
GCGCCTGTTA ACCAGATAAC CAGTGCGGAG GACGGTACTG GCACTGGTAA GGTGGGCACC 
ACTCTTTACG TGGCCCCCGA GTTGACAGGA AATGCTTCCA AGTCTGTGTA CAACCAGAAG 
GTTGATATGT ACACCCTGGG AGTCATTCTC TTCGAGATGT GCCAACCGCC ATTCGACACA 
AGCATGGAGC GCGCTCAGAC CATTATGGCC CTCAGGAATG TTTCCATCAA TATACCGGAT 
GCCATGCTCA AGGATCCGAA GTATGAGAAG ACGGTGAAGA TGCTTCAGTG GCTTCTTAAC 
CACGATCCAG CCCAGAGACC TACGGCCGAG GAGCTGCTCA TCTCAGACCT GGTTCCACCA 
GCCCAGTTGG AGGCCAATGA GCTGCAAGAA ATGCTGCGTC ACGCCCTCGC CAATCCGCAG 
AGCAAGGCCT ACAAGAACCT GGTTGCCCGG TGTCTTCAGC AGGAGAGCGA CGAGGTGCTG 
GAACACACCT ACCACTTGGG CAGCAGTCGA GCTATGAAGT CCTGGAACTC GGCAATCATA 
ATCGATGATA TAGTGTCCCT GAATCCCGTG ATTGAGTTTG TTAAGGCCAA GGTCGTGAAT 
CTATTCCGCA AGCACGGAGC TATAGAGGTG GACTCACCGC TCCTGTCCCC ACTTTCCGCA 
AGAAACAGCA GTGCGAATGC GAACGCCAAT GCCGTGCACC TGATGACGCA CTCGGGATGT 
GTGGTGGTGC TACCCTGTGA TCTGCGGACA CAGTTTGCCC GCCACGTGAC CATGAACAGC 
GTGAATCTCA TCCGGCGCTA TTGTGTGGAC CGTGTATATC GGGAGGAGCG AGTGTTCAAT 
TTCCATCCCA AACAAAGCTA CGACTGCTCT TTCGATATTA TTGCGCCCAC AACTGGAAGC 
CATCTAGTGG ATGCGGAGCT GCTATCGTTG GCTTTTGAAA TCACCAGTGA ACTGCCCCGT 
TTACGGGAAA AGAATCTGGC GATCCGCATG AATCACACTA ATCTGTTGAG GGCCATCCTT 
ATCTTCTGCA ATGTTCCAAA GGCTCAGTAT GAAGCACTTT TCGAGGGCAC CATGGACTTT 
ATCGAGTCGC GCATCTCACG CTTCCAATTT CACTCGAGTA TAACAGGGAT TATGGAAAAG 
TCGCGGACAT CTGCTCAAAC TCTAATGGAC ATGTTGTTAG CCAACTTTCT GTTGACTGGT 
TCCAGAAGCA CGGTGGACGA TTCCGCGCTG AAATCGTTGA TGCGGGGAAA GGGCGAAGCG 
GCTTCACTGG CAAGAGGAGC ACTACGGGAA CTTGAAACAG TTGTGGGACT GGCTTACAGT 
CTAGGCGTAA AATGTCCTAT TCACATATGG GCTGGCTTGC CCATCAGTTT CGATAGTGCC 
AGCAATGGTG GAATTGTGTG GCAGATGACC GCTGACCTAA AGCCGAATCG CTCTGGTCAT 
CCTTCAGTTC TGGCGATCGG GCAGAGATAC GATTCCATGC TGCATGAATT CCAGAAGCAG 
GCGCAGAAGT TTAATCCAGC CTTGCCGGCA CGAGGAGTTC TGAGCGGAGC GGGTTTGACG 
TTTTCCCTAG ACAAACTAGT AGCTGCTGTG GGTGTGGAAT ACGCTAAGGA TTGTCGGGCG 
ATAGACGTGG GCATTTGCGT GTGTGGAACG CGTCCGCCGC TGAAGGATGT GACCTACATC 
ATGCGCCTGC TGTGGTCGGT TGGCATTCGT TGTGGCATTG TGGAGGCCGC CAGCGAATTG 
GGTGATGAAG CACAGGATCT TGCTCGCCTT GGGGCGTTAC ACGTCATCCT AGTGGCGGAA 
AATGGATCCT TGAGAGTGCG CTCTTTCGAG AGGGACCGCT TTCAGGAGCG TCACCTAACA 
AGAGCCGAAC TGGTGGAGTT CATCCAGAAG ATGCTGCGAA CTGATGGACT CAATGGAACC 
ACCGTCGACA ACTTCAGCCA GTTGTCAACC ATGGGTAGTG GCGACAGCAG AAGCAGTGGT 
GGCAAGGAAC GGGAACGCGG CGAGAATGGT CTGAGCACCT CCGCATCGAA TGCCACCATT 
AAGAACAGCT ATAGCCAGCT GCCGAATCTC CAGGTGACGT TTCTCACCCA CGACAAGCCT 
ACGGCGAACT ACAAAAGACG CCTGGAGAAC CAGGTGGCGC AGCAGATGAG CTCAACGCTA 
TCGCAGTTCC TCAAGAAAGA GACCTTTGTG GTCCTGGTGG TGGAGTTGCC GCCTGCTGTT 
GTAAATGCCA TTGTAGGGGC CATCAATCCA CGAGAAGTAC GCAAGAGAGA GACTGAACCG 
GAAATCAACT TTGTGATCGA GCGATTTTCG AAATATAAGC GCTATATATC CGAGATAAAC 
GAAGAGATTG TGGACTATCT GAGCGATGCC AAGACTCCCA TTGTGGCCTT GTACAGCATT 
TCCGATTCCT ACTACCGCGT TATTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6554743 ( XM_001981050.3 ) cDNA ORF clone, Drosophila erecta -> XP_001981086.1 Drosophila erecta eIF-2-alpha kinase GCN2 (LOC6554743), mRNA.

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CloneID	ODi10497
Clone ID Related Accession (Same CDS sequence)	XM_001981050.2 , XM_001981050.3
Accession Version	XM_001981050.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	eIF-2-alpha kinase GCN2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825194.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_001981050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG AAAAGGCTAA GGAATCCTTC CGCGAGCGGC AAGCGCAGGA GTTGGAGGTT 
ATAAAGTCCA TCTTTGGCGG TGATGTGGAG GACCTGCGAC CCCAGGCGAA TCCTTCCCTG 
TGGAAGCCAA CGGACATAAG GATACAGCTT ACCCCACTTA GGGACTCCTC CAATGGACTG 
GAAACTTATG TGTGCACCAA ACTGCATGTC ACCTGTCCTT CAAAGTATCC AAAATTGCCC 
CCTAAGATAT GTCTCGAGGA GTCCAAGGGC ATGTCGGATC AGTTGTTGGA GGCCCTACGC 
AACCAACTGC AGGCTCAATC CCAAGAGCTG CGTGGGGAAG TGATGATTTA TGAACTGGCC 
CAGACCGTTC AGGCCTTTCT CCTCGAACAC AACAAACCGC CAAAGGGCTC TTTCTACGAT 
CAGATGCTAC AAGACAAACA GAAGCGCGAC CAGGAGCTAC AGGACATGCA GCGGCAGCGG 
GAGTCTGTGC AACGCCAAAC ACTCATCGAC GAGGTGGAGC GGCGCAAAGA GATGTTTAAA 
ACAGAGGCTA AAAGGCGAGG CGAACCAAGA CGCAGCATGA GCGAGTCTAA CCCACGGCAT 
CCTAGCTCGT CGGAGAGCTC CGAGAACTCC TCGCCCTACT ATCGTGGTCA CATCTACCCC 
TCCAAATGCC TCGATCACCG CAACACGGAG ACGCTCTACT TCCACAAGAT GGGCAGGCAA 
ATTCAACGAG GATGCTGCGT TGGTCATTCC CAACGAGGGT GCATCGCCTA CACTGGCATA 
GATATGCACT GCGGCCAGTT GCTCTATATC ACAGAATGGA ACATCAAGTA CAGCCAACTG 
GAGCAGCCGT GCATCGGCGG AGGCAAGTGC CACTGGAGCA GCGAGTCAAA ATGCATGGGC 
AGTCATCGTG TGGACGAAGT GATCGCCTCC ATTGAAAAGC AGGTGTCCTC GCTGGCCCAG 
CTGCAGCACA AAAATCTCGT CTCGTACGAG TGCGTTCTTT GCATCAAGCG GAAGGAAGGC 
TTACTTGTGT ATTTGGTCCA GGACTTCCTC CTCGGCACCA GCGTGTTCAG CATATCCTCC 
TCGCTGGGTT GGTGCATGGA CGGCGCACGA ATGGTTGCAC GCGGCGTGCT GGATGCCTTA 
ATATTTCTTC ACAACAAGGG TGTATCCCAC AGTCACTTGC TCGACAGCAC TGTTTTTATG 
GACAACACAG GCAATGTTCG TGTATCAGAC TTTTCGCTAG TTCCCAATCT TTTAGAACTT 
CTGAGCGGAG CTGGCCAAAG CAGCAGTTGC GGGGACTTAC CCGCGTTAGG TGCCCTGGTG 
GAGAGCTTGA TGCCCACTAA CAGTTATGAG ATGCGCGACT TTGTGGACAA ATGCAACTCG 
GACCGCACGC TTTCCGCATC AGAGTTGTTG GAGCATCCAT TCTTACGCTT TTACGTAGAC 
AATGGCCAGC AGCAAGTGAT GCCATTAACA CAGCAGCAGC ATCCGAATAC CGTCCAGCGC 
ACTGGACCCG CCATGCCCTA TCAGATACCG ACACTGGCCT TAAGCCAATC CCGCCTGCGA 
ACAGAATTCG AGGTGCTCAT GTACTTGGGA AAGGGTGCCT ATGGTGACGT GCTAAAAGTG 
CGCAACATTT TGGACAACCG AGAGTACGCC ATCAAGCGCA TACCGTTGCC CGCGAGGAGT 
CGCCAGTTGT ACAAAAAGAT GACGCGCGAG GTGGAGCTGC TTTCGCGGCT GAATCATGAG 
AACGTTGTTC GATACTTTAA CAGTTGGATA GAGAGTGTGG ATGACGCAGA TGCCGCTGAG 
ATGGATAAAT TGCTGGGTGG TGAGTGGTCC CAGAGTCAGC AGGACCTTAG CGTGAAACCT 
GCCAAGTCAC CACAGCTGGG GCCCACTTTA GAGGAGGATG AGGATGAAGA GGACAGCTCA 
TCCAGCATGT GGAACGGTTA CATACCCAAC ATGGAGGACT CCGACTCGGA TGGCATTGAA 
TTTGTGGACT CCAACGGCAA AGTGGCTGTC TACGACGACG AAGAACAAGA GGAATCCACC 
AGAGGCAGGA CTAATTCGCC CAGACCTTTG ATGCAGGTCA TGTACATCCA GATGGAGTTC 
TGCGAGAAAT GCACACTTCG CACCGCAATC GATGACAATC TTTTCAATGA CACGGATCGT 
CTGTGGCGTT TATTTCGGGA GATTGCCGAG GGCCTCGCGC ATATTCACCA GCAAGGCATT 
ATTCATCGGG ACCTAAAGCC CGTTAACATT TTCTTGGACT CGCACGATCA GATCAAGATC 
GGTGACTTTG GTCTGGCCAC CACCAGTTTT TTGGCCCTGC AGGCCCACGA CACAGCACCT 
GCGCCTGTTA ACCAGATAAC CAGTGCGGAG GACGGTACTG GCACTGGTAA GGTGGGCACC 
ACTCTTTACG TGGCCCCCGA GTTGACAGGA AATGCTTCCA AGTCTGTGTA CAACCAGAAG 
GTTGATATGT ACACCCTGGG AGTCATTCTC TTCGAGATGT GCCAACCGCC ATTCGACACA 
AGCATGGAGC GCGCTCAGAC CATTATGGCC CTCAGGAATG TTTCCATCAA TATACCGGAT 
GCCATGCTCA AGGATCCGAA GTATGAGAAG ACGGTGAAGA TGCTTCAGTG GCTTCTTAAC 
CACGATCCAG CCCAGAGACC TACGGCCGAG GAGCTGCTCA TCTCAGACCT GGTTCCACCA 
GCCCAGTTGG AGGCCAATGA GCTGCAAGAA ATGCTGCGTC ACGCCCTCGC CAATCCGCAG 
AGCAAGGCCT ACAAGAACCT GGTTGCCCGG TGTCTTCAGC AGGAGAGCGA CGAGGTGCTG 
GAACACACCT ACCACTTGGG CAGCAGTCGA GCTATGAAGT CCTGGAACTC GGCAATCATA 
ATCGATGATA TAGTGTCCCT GAATCCCGTG ATTGAGTTTG TTAAGGCCAA GGTCGTGAAT 
CTATTCCGCA AGCACGGAGC TATAGAGGTG GACTCACCGC TCCTGTCCCC ACTTTCCGCA 
AGAAACAGCA GTGCGAATGC GAACGCCAAT GCCGTGCACC TGATGACGCA CTCGGGATGT 
GTGGTGGTGC TACCCTGTGA TCTGCGGACA CAGTTTGCCC GCCACGTGAC CATGAACAGC 
GTGAATCTCA TCCGGCGCTA TTGTGTGGAC CGTGTATATC GGGAGGAGCG AGTGTTCAAT 
TTCCATCCCA AACAAAGCTA CGACTGCTCT TTCGATATTA TTGCGCCCAC AACTGGAAGC 
CATCTAGTGG ATGCGGAGCT GCTATCGTTG GCTTTTGAAA TCACCAGTGA ACTGCCCCGT 
TTACGGGAAA AGAATCTGGC GATCCGCATG AATCACACTA ATCTGTTGAG GGCCATCCTT 
ATCTTCTGCA ATGTTCCAAA GGCTCAGTAT GAAGCACTTT TCGAGGGCAC CATGGACTTT 
ATCGAGTCGC GCATCTCACG CTTCCAATTT CACTCGAGTA TAACAGGGAT TATGGAAAAG 
TCGCGGACAT CTGCTCAAAC TCTAATGGAC ATGTTGTTAG CCAACTTTCT GTTGACTGGT 
TCCAGAAGCA CGGTGGACGA TTCCGCGCTG AAATCGTTGA TGCGGGGAAA GGGCGAAGCG 
GCTTCACTGG CAAGAGGAGC ACTACGGGAA CTTGAAACAG TTGTGGGACT GGCTTACAGT 
CTAGGCGTAA AATGTCCTAT TCACATATGG GCTGGCTTGC CCATCAGTTT CGATAGTGCC 
AGCAATGGTG GAATTGTGTG GCAGATGACC GCTGACCTAA AGCCGAATCG CTCTGGTCAT 
CCTTCAGTTC TGGCGATCGG GCAGAGATAC GATTCCATGC TGCATGAATT CCAGAAGCAG 
GCGCAGAAGT TTAATCCAGC CTTGCCGGCA CGAGGAGTTC TGAGCGGAGC GGGTTTGACG 
TTTTCCCTAG ACAAACTAGT AGCTGCTGTG GGTGTGGAAT ACGCTAAGGA TTGTCGGGCG 
ATAGACGTGG GCATTTGCGT GTGTGGAACG CGTCCGCCGC TGAAGGATGT GACCTACATC 
ATGCGCCTGC TGTGGTCGGT TGGCATTCGT TGTGGCATTG TGGAGGCCGC CAGCGAATTG 
GGTGATGAAG CACAGGATCT TGCTCGCCTT GGGGCGTTAC ACGTCATCCT AGTGGCGGAA 
AATGGATCCT TGAGAGTGCG CTCTTTCGAG AGGGACCGCT TTCAGGAGCG TCACCTAACA 
AGAGCCGAAC TGGTGGAGTT CATCCAGAAG ATGCTGCGAA CTGATGGACT CAATGGAACC 
ACCGTCGACA ACTTCAGCCA GTTGTCAACC ATGGGTAGTG GCGACAGCAG AAGCAGTGGT 
GGCAAGGAAC GGGAACGCGG CGAGAATGGT CTGAGCACCT CCGCATCGAA TGCCACCATT 
AAGAACAGCT ATAGCCAGCT GCCGAATCTC CAGGTGACGT TTCTCACCCA CGACAAGCCT 
ACGGCGAACT ACAAAAGACG CCTGGAGAAC CAGGTGGCGC AGCAGATGAG CTCAACGCTA 
TCGCAGTTCC TCAAGAAAGA GACCTTTGTG GTCCTGGTGG TGGAGTTGCC GCCTGCTGTT 
GTAAATGCCA TTGTAGGGGC CATCAATCCA CGAGAAGTAC GCAAGAGAGA GACTGAACCG 
GAAATCAACT TTGTGATCGA GCGATTTTCG AAATATAAGC GCTATATATC CGAGATAAAC 
GAAGAGATTG TGGACTATCT GAGCGATGCC AAGACTCCCA TTGTGGCCTT GTACAGCATT 
TCCGATTCCT ACTACCGCGT TATTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001981086.1
CDS	371..5140
Translation	MADEKAKESFRERQAQELEVIKSIFGGDVEDLRPQANPSLWKPTDIRIQLTPLRDSSNGLETYVCTKLHVTCPSKYPKLPPKICLEESKGMSDQLLEALRNQLQAQSQELRGEVMIYELAQTVQAFLLEHNKPPKGSFYDQMLQDKQKRDQELQDMQRQRESVQRQTLIDEVERRKEMFKTEAKRRGEPRRSMSESNPRHPSSSESSENSSPYYRGHIYPSKCLDHRNTETLYFHKMGRQIQRGCCVGHSQRGCIAYTGIDMHCGQLLYITEWNIKYSQLEQPCIGGGKCHWSSESKCMGSHRVDEVIASIEKQVSSLAQLQHKNLVSYECVLCIKRKEGLLVYLVQDFLLGTSVFSISSSLGWCMDGARMVARGVLDALIFLHNKGVSHSHLLDSTVFMDNTGNVRVSDFSLVPNLLELLSGAGQSSSCGDLPALGALVESLMPTNSYEMRDFVDKCNSDRTLSASELLEHPFLRFYVDNGQQQVMPLTQQQHPNTVQRTGPAMPYQIPTLALSQSRLRTEFEVLMYLGKGAYGDVLKVRNILDNREYAIKRIPLPARSRQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDADAAEMDKLLGGEWSQSQQDLSVKPAKSPQLGPTLEEDEDEEDSSSSMWNGYIPNMEDSDSDGIEFVDSNGKVAVYDDEEQEESTRGRTNSPRPLMQVMYIQMEFCEKCTLRTAIDDNLFNDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHDQIKIGDFGLATTSFLALQAHDTAPAPVNQITSAEDGTGTGKVGTTLYVAPELTGNASKSVYNQKVDMYTLGVILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKTVKMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALANPQSKAYKNLVARCLQQESDEVLEHTYHLGSSRAMKSWNSAIIIDDIVSLNPVIEFVKAKVVNLFRKHGAIEVDSPLLSPLSARNSSANANANAVHLMTHSGCVVVLPCDLRTQFARHVTMNSVNLIRRYCVDRVYREERVFNFHPKQSYDCSFDIIAPTTGSHLVDAELLSLAFEITSELPRLREKNLAIRMNHTNLLRAILIFCNVPKAQYEALFEGTMDFIESRISRFQFHSSITGIMEKSRTSAQTLMDMLLANFLLTGSRSTVDDSALKSLMRGKGEAASLARGALRELETVVGLAYSLGVKCPIHIWAGLPISFDSASNGGIVWQMTADLKPNRSGHPSVLAIGQRYDSMLHEFQKQAQKFNPALPARGVLSGAGLTFSLDKLVAAVGVEYAKDCRAIDVGICVCGTRPPLKDVTYIMRLLWSVGIRCGIVEAASELGDEAQDLARLGALHVILVAENGSLRVRSFERDRFQERHLTRAELVEFIQKMLRTDGLNGTTVDNFSQLSTMGSGDSRSSGGKERERGENGLSTSASNATIKNSYSQLPNLQVTFLTHDKPTANYKRRLENQVAQQMSSTLSQFLKKETFVVLVVELPPAVVNAIVGAINPREVRKRETEPEINFVIERFSKYKRYISEINEEIVDYLSDAKTPIVALYSISDSYYRVII

Target ORF information:

RefSeq Version	XM_001981050.3
Organism	Drosophila erecta
Definition	Drosophila erecta eIF-2-alpha kinase GCN2 (LOC6554743), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001981050.3

ORF Insert Sequence:

ATGGCGGATG AAAAGGCTAA GGAATCCTTC CGCGAGCGGC AAGCGCAGGA GTTGGAGGTT 
ATAAAGTCCA TCTTTGGCGG TGATGTGGAG GACCTGCGAC CCCAGGCGAA TCCTTCCCTG 
TGGAAGCCAA CGGACATAAG GATACAGCTT ACCCCACTTA GGGACTCCTC CAATGGACTG 
GAAACTTATG TGTGCACCAA ACTGCATGTC ACCTGTCCTT CAAAGTATCC AAAATTGCCC 
CCTAAGATAT GTCTCGAGGA GTCCAAGGGC ATGTCGGATC AGTTGTTGGA GGCCCTACGC 
AACCAACTGC AGGCTCAATC CCAAGAGCTG CGTGGGGAAG TGATGATTTA TGAACTGGCC 
CAGACCGTTC AGGCCTTTCT CCTCGAACAC AACAAACCGC CAAAGGGCTC TTTCTACGAT 
CAGATGCTAC AAGACAAACA GAAGCGCGAC CAGGAGCTAC AGGACATGCA GCGGCAGCGG 
GAGTCTGTGC AACGCCAAAC ACTCATCGAC GAGGTGGAGC GGCGCAAAGA GATGTTTAAA 
ACAGAGGCTA AAAGGCGAGG CGAACCAAGA CGCAGCATGA GCGAGTCTAA CCCACGGCAT 
CCTAGCTCGT CGGAGAGCTC CGAGAACTCC TCGCCCTACT ATCGTGGTCA CATCTACCCC 
TCCAAATGCC TCGATCACCG CAACACGGAG ACGCTCTACT TCCACAAGAT GGGCAGGCAA 
ATTCAACGAG GATGCTGCGT TGGTCATTCC CAACGAGGGT GCATCGCCTA CACTGGCATA 
GATATGCACT GCGGCCAGTT GCTCTATATC ACAGAATGGA ACATCAAGTA CAGCCAACTG 
GAGCAGCCGT GCATCGGCGG AGGCAAGTGC CACTGGAGCA GCGAGTCAAA ATGCATGGGC 
AGTCATCGTG TGGACGAAGT GATCGCCTCC ATTGAAAAGC AGGTGTCCTC GCTGGCCCAG 
CTGCAGCACA AAAATCTCGT CTCGTACGAG TGCGTTCTTT GCATCAAGCG GAAGGAAGGC 
TTACTTGTGT ATTTGGTCCA GGACTTCCTC CTCGGCACCA GCGTGTTCAG CATATCCTCC 
TCGCTGGGTT GGTGCATGGA CGGCGCACGA ATGGTTGCAC GCGGCGTGCT GGATGCCTTA 
ATATTTCTTC ACAACAAGGG TGTATCCCAC AGTCACTTGC TCGACAGCAC TGTTTTTATG 
GACAACACAG GCAATGTTCG TGTATCAGAC TTTTCGCTAG TTCCCAATCT TTTAGAACTT 
CTGAGCGGAG CTGGCCAAAG CAGCAGTTGC GGGGACTTAC CCGCGTTAGG TGCCCTGGTG 
GAGAGCTTGA TGCCCACTAA CAGTTATGAG ATGCGCGACT TTGTGGACAA ATGCAACTCG 
GACCGCACGC TTTCCGCATC AGAGTTGTTG GAGCATCCAT TCTTACGCTT TTACGTAGAC 
AATGGCCAGC AGCAAGTGAT GCCATTAACA CAGCAGCAGC ATCCGAATAC CGTCCAGCGC 
ACTGGACCCG CCATGCCCTA TCAGATACCG ACACTGGCCT TAAGCCAATC CCGCCTGCGA 
ACAGAATTCG AGGTGCTCAT GTACTTGGGA AAGGGTGCCT ATGGTGACGT GCTAAAAGTG 
CGCAACATTT TGGACAACCG AGAGTACGCC ATCAAGCGCA TACCGTTGCC CGCGAGGAGT 
CGCCAGTTGT ACAAAAAGAT GACGCGCGAG GTGGAGCTGC TTTCGCGGCT GAATCATGAG 
AACGTTGTTC GATACTTTAA CAGTTGGATA GAGAGTGTGG ATGACGCAGA TGCCGCTGAG 
ATGGATAAAT TGCTGGGTGG TGAGTGGTCC CAGAGTCAGC AGGACCTTAG CGTGAAACCT 
GCCAAGTCAC CACAGCTGGG GCCCACTTTA GAGGAGGATG AGGATGAAGA GGACAGCTCA 
TCCAGCATGT GGAACGGTTA CATACCCAAC ATGGAGGACT CCGACTCGGA TGGCATTGAA 
TTTGTGGACT CCAACGGCAA AGTGGCTGTC TACGACGACG AAGAACAAGA GGAATCCACC 
AGAGGCAGGA CTAATTCGCC CAGACCTTTG ATGCAGGTCA TGTACATCCA GATGGAGTTC 
TGCGAGAAAT GCACACTTCG CACCGCAATC GATGACAATC TTTTCAATGA CACGGATCGT 
CTGTGGCGTT TATTTCGGGA GATTGCCGAG GGCCTCGCGC ATATTCACCA GCAAGGCATT 
ATTCATCGGG ACCTAAAGCC CGTTAACATT TTCTTGGACT CGCACGATCA GATCAAGATC 
GGTGACTTTG GTCTGGCCAC CACCAGTTTT TTGGCCCTGC AGGCCCACGA CACAGCACCT 
GCGCCTGTTA ACCAGATAAC CAGTGCGGAG GACGGTACTG GCACTGGTAA GGTGGGCACC 
ACTCTTTACG TGGCCCCCGA GTTGACAGGA AATGCTTCCA AGTCTGTGTA CAACCAGAAG 
GTTGATATGT ACACCCTGGG AGTCATTCTC TTCGAGATGT GCCAACCGCC ATTCGACACA 
AGCATGGAGC GCGCTCAGAC CATTATGGCC CTCAGGAATG TTTCCATCAA TATACCGGAT 
GCCATGCTCA AGGATCCGAA GTATGAGAAG ACGGTGAAGA TGCTTCAGTG GCTTCTTAAC 
CACGATCCAG CCCAGAGACC TACGGCCGAG GAGCTGCTCA TCTCAGACCT GGTTCCACCA 
GCCCAGTTGG AGGCCAATGA GCTGCAAGAA ATGCTGCGTC ACGCCCTCGC CAATCCGCAG 
AGCAAGGCCT ACAAGAACCT GGTTGCCCGG TGTCTTCAGC AGGAGAGCGA CGAGGTGCTG 
GAACACACCT ACCACTTGGG CAGCAGTCGA GCTATGAAGT CCTGGAACTC GGCAATCATA 
ATCGATGATA TAGTGTCCCT GAATCCCGTG ATTGAGTTTG TTAAGGCCAA GGTCGTGAAT 
CTATTCCGCA AGCACGGAGC TATAGAGGTG GACTCACCGC TCCTGTCCCC ACTTTCCGCA 
AGAAACAGCA GTGCGAATGC GAACGCCAAT GCCGTGCACC TGATGACGCA CTCGGGATGT 
GTGGTGGTGC TACCCTGTGA TCTGCGGACA CAGTTTGCCC GCCACGTGAC CATGAACAGC 
GTGAATCTCA TCCGGCGCTA TTGTGTGGAC CGTGTATATC GGGAGGAGCG AGTGTTCAAT 
TTCCATCCCA AACAAAGCTA CGACTGCTCT TTCGATATTA TTGCGCCCAC AACTGGAAGC 
CATCTAGTGG ATGCGGAGCT GCTATCGTTG GCTTTTGAAA TCACCAGTGA ACTGCCCCGT 
TTACGGGAAA AGAATCTGGC GATCCGCATG AATCACACTA ATCTGTTGAG GGCCATCCTT 
ATCTTCTGCA ATGTTCCAAA GGCTCAGTAT GAAGCACTTT TCGAGGGCAC CATGGACTTT 
ATCGAGTCGC GCATCTCACG CTTCCAATTT CACTCGAGTA TAACAGGGAT TATGGAAAAG 
TCGCGGACAT CTGCTCAAAC TCTAATGGAC ATGTTGTTAG CCAACTTTCT GTTGACTGGT 
TCCAGAAGCA CGGTGGACGA TTCCGCGCTG AAATCGTTGA TGCGGGGAAA GGGCGAAGCG 
GCTTCACTGG CAAGAGGAGC ACTACGGGAA CTTGAAACAG TTGTGGGACT GGCTTACAGT 
CTAGGCGTAA AATGTCCTAT TCACATATGG GCTGGCTTGC CCATCAGTTT CGATAGTGCC 
AGCAATGGTG GAATTGTGTG GCAGATGACC GCTGACCTAA AGCCGAATCG CTCTGGTCAT 
CCTTCAGTTC TGGCGATCGG GCAGAGATAC GATTCCATGC TGCATGAATT CCAGAAGCAG 
GCGCAGAAGT TTAATCCAGC CTTGCCGGCA CGAGGAGTTC TGAGCGGAGC GGGTTTGACG 
TTTTCCCTAG ACAAACTAGT AGCTGCTGTG GGTGTGGAAT ACGCTAAGGA TTGTCGGGCG 
ATAGACGTGG GCATTTGCGT GTGTGGAACG CGTCCGCCGC TGAAGGATGT GACCTACATC 
ATGCGCCTGC TGTGGTCGGT TGGCATTCGT TGTGGCATTG TGGAGGCCGC CAGCGAATTG 
GGTGATGAAG CACAGGATCT TGCTCGCCTT GGGGCGTTAC ACGTCATCCT AGTGGCGGAA 
AATGGATCCT TGAGAGTGCG CTCTTTCGAG AGGGACCGCT TTCAGGAGCG TCACCTAACA 
AGAGCCGAAC TGGTGGAGTT CATCCAGAAG ATGCTGCGAA CTGATGGACT CAATGGAACC 
ACCGTCGACA ACTTCAGCCA GTTGTCAACC ATGGGTAGTG GCGACAGCAG AAGCAGTGGT 
GGCAAGGAAC GGGAACGCGG CGAGAATGGT CTGAGCACCT CCGCATCGAA TGCCACCATT 
AAGAACAGCT ATAGCCAGCT GCCGAATCTC CAGGTGACGT TTCTCACCCA CGACAAGCCT 
ACGGCGAACT ACAAAAGACG CCTGGAGAAC CAGGTGGCGC AGCAGATGAG CTCAACGCTA 
TCGCAGTTCC TCAAGAAAGA GACCTTTGTG GTCCTGGTGG TGGAGTTGCC GCCTGCTGTT 
GTAAATGCCA TTGTAGGGGC CATCAATCCA CGAGAAGTAC GCAAGAGAGA GACTGAACCG 
GAAATCAACT TTGTGATCGA GCGATTTTCG AAATATAAGC GCTATATATC CGAGATAAAC 
GAAGAGATTG TGGACTATCT GAGCGATGCC AAGACTCCCA TTGTGGCCTT GTACAGCATT 
TCCGATTCCT ACTACCGCGT TATTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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