LOC6544975 cDNA ORF clone, Drosophila erecta

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC6544975
Entrez Gene ID	6544975
Full Name	GG14367 gene product from transcript GG14367-RB
Synonyms	GG14367,dere_GLEANR_14543
Gene Type	protein-coding
Organism	Drosophila erecta

mRNA and Protein(s)

mRNA	Protein	Name
XM_001971536.2	XP_001971572.1	uncharacterized protein, isoform A
XM_015157920.1	XP_015013406.1	uncharacterized protein, isoform B
XM_015157920.2	XP_015013406.1	ubiquitin-protein ligase E3B isoform X2
XM_001971536.3	XP_001971572.1	ubiquitin-protein ligase E3B isoform X1
XM_026977387.1	XP_026833188.1	ubiquitin-protein ligase E3B isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC6544975 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC6544975 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODi03240	XM_026977387.1 Latest version!	Drosophila erecta ubiquitin-protein ligase E3B (LOC6544975), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODi03240	XM_001971536.3 Latest version!	Drosophila erecta ubiquitin-protein ligase E3B (LOC6544975), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODi38167	XM_015157920.2 Latest version!	Drosophila erecta ubiquitin-protein ligase E3B (LOC6544975), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODi38167	XM_015157920.1	Drosophila erecta uncharacterized protein, transcript variant B (Dere\GG14367), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODi03240	XM_001971536.2	Drosophila erecta uncharacterized protein, transcript variant A (Dere\GG14367), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC6544975 ( XM_001971536.2 ) cDNA ORF clone, Drosophila erecta -> XP_001971572.1 Drosophila erecta uncharacterized protein, transcript variant A (Dere\GG14367), mRNA.

Price & Availability↑

CloneID	ODi03240
Clone ID Related Accession (Same CDS sequence)	XM_001971536.2 , XM_001971536.3 , XM_026977387.1
Accession Version	XM_001971536.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956549). On Dec 21, 2015 this sequence version replaced XM_001971536.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGCCC AAAACCAAAA GACCAGCTTC CTGGAGCAGA CTAAGGCGGC GCGGGAGGAG 
CGGGCGCTGG AGAAGCGCCG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAAGGG 
TATGCGGCGC GTAGGAAGTA CCAGAAACGG ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCAGG ACGATAAGGA TACGGAACTG GAACTTGTGG CCAGCAACGT CTATCCGGTG 
CTCCGTCGCT ACCTCACCCA GATCAAGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGTCGTTA CATCATCAAG GCAATGGAGG CGGAGAGCAC CAAGCTATCC 
TATGCCGCCC TCTGTCTCTT CAAGGAACGC AGCCTGCCCT GGATCGCGCA CATTAAGATA 
CTGCTCACGA ACTGCCTAAC ATTGCTGCCG GAGCTGAAGC CGGAGAACCA TGCCGACAGC 
TTGTCCCTTG CCCTCTTCCT GCACACACTG GTGGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT CGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGCTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTC TACAAAATCA TGCGGCTCGT TTTGCTGCGC 
GGCACTGTGC GCGAGGAGTT GTCTGTGAAG CCCGTCACCC TGGTGGCCAT CATTACGCTA 
TGCCTTCGTC CCCTGATCGA TGGCAACTTT AGTCGAAATC TACTGGGCAA ATTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCACC AGAGTGTGCC GCAGTGCCTG 
GAACAGTTCT CATCTTTGGG GCTGCTTAAA AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGATG AATTCGGCAC GAGCATGCCG GGCACAAAAT CCCTGGCTTT TCTGGGAAAC 
ATTGTGAATT TGTTCAACAT TGATGGCCAA GGGGAGTCCA AGGAGCTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACCAAGGGA 
GTGTTCACTC AGTGGCACGA GCTGTTAGGA TGGCATACTC CGGGTCCAGA GCCTGCACAG 
AATCAGAATG TGGCCCTGAT TAAGAAGCAG TTTCACATGT TGTGGGATCA TCGCTGCATA 
AAGCTGTTGC TGGGAGATCT CTTGAAGCAA ATCAATATCA ACTACGAGCG AATCGAGTTC 
CAATCTCCCC AACAACCATC CACATCGAAT CTGCTGCGTC GGGCCCTGGA GCGTAGCTCC 
ACTAGAGGTG TTAACCTAAT GGGAGTAGCG AGCAGCAAGC AGGCTAAGCA GCAGTGGCGC 
AAGCTGGACA ATGCGGAGGT GGTGCAGGTG TCCCGAATCT GCGGCATGTA CTATGCCGCC 
CTCAACACCC TTTCCCAAAT GAAGCTGGAC ATCCTGACTG GCATTTGCTA CAACGACAAT 
GTGCTGTATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGCGG CATGAAGGAG 
TATCTGGAGC TGCTCAAATC CGAGTCAAAC ATGCAGAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAATACGA AATGTACACG 
GAGCAGAATC CCTTTAAACT TAACGACTAT GTGATGCTGA CCTACTTCCT AAATAATATT 
CTCTACAAAC TTATTAACGA TAATCTTCTG GCAGGTGCCA AGAACATTGT CCAGAATCCG 
GTTTTCCTCT CGCTGCACAC GCTGATGCTT TGCTTGTACC GCCGTGATTG CCGTCGCCCC 
TTCACGCCGC CCAACCACTG GCTGATACCG GAGGTCAAAC CCTCCACGTT CATCAACGAC 
CTGGAAAAGG CCAAGAGGAA CGCGATGCTG CTGCTCGCCA AGATGCCTCA CATTATACCG 
CACGAGGATC GGGTCAAGCT ATTCCGGAAG TTCGTGCAGA ATGAGAAGGC GGTGATGGGT 
CTGACGGAGA GTGCCTGCGC TTCTCCTCGC TCCGCACTGA TTGTCATCCA TCGCGATCGT 
ATTGTGGAGG ATGGATACCG GCAACTGGCC GCCCAGCCCA CGCAAGCCCT AAAAGGTGTT 
ATCCGCGTAC GTTTCATCAA TCAACAGGGA CTGCACGAGG CAGGAATCGA TCAGGATGGA 
GTGTTCAAGG AATTCCTTGA GGAGACCATC AAAAAGGTGT TCGACCCGTC GCTGAATCTC 
TTCAAAACCA CGTCGGATCA GCGGCTATAT CCCTCGCCCA TTTCCTACGT CCAGGACAAT 
CATTTGCAGC TCTTCGAGTT CGTGGGCCGC ATGCTGGGCA AGGCGGTGTA CGAAGGCATC 
GTGGTGGACG TCCCGTTTGC CTCCTTCTTC CTCTCCCAGC TTCTCGGCCA GACGCAACAG 
GCGCTGTACT CCTGCATGGA CGAGCTGCCG TCGCTGGACA ACGAGTTGTA TCGCAGTCTA 
ACCTTCATCA AACACTATAA GCAAGATGTG TCCGATCTGA ACCTTACCTT TTCCGTGGAC 
CAGGATGTGA TGGGCAAAAT TGTCACTCTG GCATTGCATC CGGGTGGCAA GTCGCGTGTG 
GTTAATGACC ACAACAAGCT GGTCTACATA CACTACATGG CATTTTTCCA CATGAACACC 
CAGATCCGCG AGCAGACGAT AGCCTTCAAT CGTGGCTTCC GCAGCATCGT CAATCCAGAA 
TGGTTGTCCC TTTTCTCGCC TCCGGAATTA CAGCGCCTGA TTTCTGGGGA CACCAGTCCA 
TTGGACTTGA AGGATCTGCG AAAGCATACA CACTATTACG GTGGCTTCCA CGACTCCCAT 
CGCGTGGTTG GCTGGCTCTG GGATATCCTG GCCAAGGATT TCACGGAGGA GGAGCGTAAG 
CTGTTCCTAA AGTTTGTTAC TAGCTGCTCA AAGCCTCCGC TTTTGGGTTT CGCCCACCTG 
GAGCCACCCT TCTCGATTCG TTGCGTGGAG GTGAGTGATG ACGAGGATAC GGGCGACACA 
ATCGGAAGTG TGATTCGCGG TTTCTTTACC ATACGTAAAA AGGATCCACT CAACCGCCTA 
CCCACCTCGT CGACCTGCTT CAACCTGCTG AAGCTGCCCA ACTACCAAAA GAAGTCCACG 
CTGAGGGACA AACTTCGCTA TGCTGTGAGC AGCAACACCG GATTCGAGCT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001971572.1
CDS	272..3508
Translation	MFAQNQKTSFLEQTKAAREERALEKRREQAAILLQSTLKGYAARRKYQKRIILDFDAMFTENQDDKDTELELVASNVYPVLRRYLTQIKLDKSDVRARERLERICRYIIKAMEAESTKLSYAALCLFKERSLPWIAHIKILLTNCLTLLPELKPENHADSLSLALFLHTLVVFTAPKSWAILRNAQFEKLQPAMQKICCNIQGHLVQHDFYKIMRLVLLRGTVREELSVKPVTLVAIITLCLRPLIDGNFSRNLLGKFLSEILSVPALIYHLHQSVPQCLEQFSSLGLLKKALSISGDVQWFDEFGTSMPGTKSLAFLGNIVNLFNIDGQGESKELAYPLLTETTTSLLELIPNTVTTKGVFTQWHELLGWHTPGPEPAQNQNVALIKKQFHMLWDHRCIKLLLGDLLKQININYERIEFQSPQQPSTSNLLRRALERSSTRGVNLMGVASSKQAKQQWRKLDNAEVVQVSRICGMYYAALNTLSQMKLDILTGICYNDNVLYDIWLLITSLGPNCGMKEYLELLKSESNMQKPQTAMLMLFCDCMTHYVTILDEYEMYTEQNPFKLNDYVMLTYFLNNILYKLINDNLLAGAKNIVQNPVFLSLHTLMLCLYRRDCRRPFTPPNHWLIPEVKPSTFINDLEKAKRNAMLLLAKMPHIIPHEDRVKLFRKFVQNEKAVMGLTESACASPRSALIVIHRDRIVEDGYRQLAAQPTQALKGVIRVRFINQQGLHEAGIDQDGVFKEFLEETIKKVFDPSLNLFKTTSDQRLYPSPISYVQDNHLQLFEFVGRMLGKAVYEGIVVDVPFASFFLSQLLGQTQQALYSCMDELPSLDNELYRSLTFIKHYKQDVSDLNLTFSVDQDVMGKIVTLALHPGGKSRVVNDHNKLVYIHYMAFFHMNTQIREQTIAFNRGFRSIVNPEWLSLFSPPELQRLISGDTSPLDLKDLRKHTHYYGGFHDSHRVVGWLWDILAKDFTEEERKLFLKFVTSCSKPPLLGFAHLEPPFSIRCVEVSDDEDTGDTIGSVIRGFFTIRKKDPLNRLPTSSTCFNLLKLPNYQKKSTLRDKLRYAVSSNTGFELS

Target ORF information:

RefSeq Version	XM_001971536.2
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant A (Dere\GG14367), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001971536.2

ORF Insert Sequence:

ATGTTTGCCC AAAACCAAAA GACCAGCTTC CTGGAGCAGA CTAAGGCGGC GCGGGAGGAG 
CGGGCGCTGG AGAAGCGCCG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAAGGG 
TATGCGGCGC GTAGGAAGTA CCAGAAACGG ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCAGG ACGATAAGGA TACGGAACTG GAACTTGTGG CCAGCAACGT CTATCCGGTG 
CTCCGTCGCT ACCTCACCCA GATCAAGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGTCGTTA CATCATCAAG GCAATGGAGG CGGAGAGCAC CAAGCTATCC 
TATGCCGCCC TCTGTCTCTT CAAGGAACGC AGCCTGCCCT GGATCGCGCA CATTAAGATA 
CTGCTCACGA ACTGCCTAAC ATTGCTGCCG GAGCTGAAGC CGGAGAACCA TGCCGACAGC 
TTGTCCCTTG CCCTCTTCCT GCACACACTG GTGGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT CGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGCTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTC TACAAAATCA TGCGGCTCGT TTTGCTGCGC 
GGCACTGTGC GCGAGGAGTT GTCTGTGAAG CCCGTCACCC TGGTGGCCAT CATTACGCTA 
TGCCTTCGTC CCCTGATCGA TGGCAACTTT AGTCGAAATC TACTGGGCAA ATTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCACC AGAGTGTGCC GCAGTGCCTG 
GAACAGTTCT CATCTTTGGG GCTGCTTAAA AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGATG AATTCGGCAC GAGCATGCCG GGCACAAAAT CCCTGGCTTT TCTGGGAAAC 
ATTGTGAATT TGTTCAACAT TGATGGCCAA GGGGAGTCCA AGGAGCTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACCAAGGGA 
GTGTTCACTC AGTGGCACGA GCTGTTAGGA TGGCATACTC CGGGTCCAGA GCCTGCACAG 
AATCAGAATG TGGCCCTGAT TAAGAAGCAG TTTCACATGT TGTGGGATCA TCGCTGCATA 
AAGCTGTTGC TGGGAGATCT CTTGAAGCAA ATCAATATCA ACTACGAGCG AATCGAGTTC 
CAATCTCCCC AACAACCATC CACATCGAAT CTGCTGCGTC GGGCCCTGGA GCGTAGCTCC 
ACTAGAGGTG TTAACCTAAT GGGAGTAGCG AGCAGCAAGC AGGCTAAGCA GCAGTGGCGC 
AAGCTGGACA ATGCGGAGGT GGTGCAGGTG TCCCGAATCT GCGGCATGTA CTATGCCGCC 
CTCAACACCC TTTCCCAAAT GAAGCTGGAC ATCCTGACTG GCATTTGCTA CAACGACAAT 
GTGCTGTATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGCGG CATGAAGGAG 
TATCTGGAGC TGCTCAAATC CGAGTCAAAC ATGCAGAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAATACGA AATGTACACG 
GAGCAGAATC CCTTTAAACT TAACGACTAT GTGATGCTGA CCTACTTCCT AAATAATATT 
CTCTACAAAC TTATTAACGA TAATCTTCTG GCAGGTGCCA AGAACATTGT CCAGAATCCG 
GTTTTCCTCT CGCTGCACAC GCTGATGCTT TGCTTGTACC GCCGTGATTG CCGTCGCCCC 
TTCACGCCGC CCAACCACTG GCTGATACCG GAGGTCAAAC CCTCCACGTT CATCAACGAC 
CTGGAAAAGG CCAAGAGGAA CGCGATGCTG CTGCTCGCCA AGATGCCTCA CATTATACCG 
CACGAGGATC GGGTCAAGCT ATTCCGGAAG TTCGTGCAGA ATGAGAAGGC GGTGATGGGT 
CTGACGGAGA GTGCCTGCGC TTCTCCTCGC TCCGCACTGA TTGTCATCCA TCGCGATCGT 
ATTGTGGAGG ATGGATACCG GCAACTGGCC GCCCAGCCCA CGCAAGCCCT AAAAGGTGTT 
ATCCGCGTAC GTTTCATCAA TCAACAGGGA CTGCACGAGG CAGGAATCGA TCAGGATGGA 
GTGTTCAAGG AATTCCTTGA GGAGACCATC AAAAAGGTGT TCGACCCGTC GCTGAATCTC 
TTCAAAACCA CGTCGGATCA GCGGCTATAT CCCTCGCCCA TTTCCTACGT CCAGGACAAT 
CATTTGCAGC TCTTCGAGTT CGTGGGCCGC ATGCTGGGCA AGGCGGTGTA CGAAGGCATC 
GTGGTGGACG TCCCGTTTGC CTCCTTCTTC CTCTCCCAGC TTCTCGGCCA GACGCAACAG 
GCGCTGTACT CCTGCATGGA CGAGCTGCCG TCGCTGGACA ACGAGTTGTA TCGCAGTCTA 
ACCTTCATCA AACACTATAA GCAAGATGTG TCCGATCTGA ACCTTACCTT TTCCGTGGAC 
CAGGATGTGA TGGGCAAAAT TGTCACTCTG GCATTGCATC CGGGTGGCAA GTCGCGTGTG 
GTTAATGACC ACAACAAGCT GGTCTACATA CACTACATGG CATTTTTCCA CATGAACACC 
CAGATCCGCG AGCAGACGAT AGCCTTCAAT CGTGGCTTCC GCAGCATCGT CAATCCAGAA 
TGGTTGTCCC TTTTCTCGCC TCCGGAATTA CAGCGCCTGA TTTCTGGGGA CACCAGTCCA 
TTGGACTTGA AGGATCTGCG AAAGCATACA CACTATTACG GTGGCTTCCA CGACTCCCAT 
CGCGTGGTTG GCTGGCTCTG GGATATCCTG GCCAAGGATT TCACGGAGGA GGAGCGTAAG 
CTGTTCCTAA AGTTTGTTAC TAGCTGCTCA AAGCCTCCGC TTTTGGGTTT CGCCCACCTG 
GAGCCACCCT TCTCGATTCG TTGCGTGGAG GTGAGTGATG ACGAGGATAC GGGCGACACA 
ATCGGAAGTG TGATTCGCGG TTTCTTTACC ATACGTAAAA AGGATCCACT CAACCGCCTA 
CCCACCTCGT CGACCTGCTT CAACCTGCTG AAGCTGCCCA ACTACCAAAA GAAGTCCACG 
CTGAGGGACA AACTTCGCTA TGCTGTGAGC AGCAACACCG GATTCGAGCT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6544975 ( XM_001971536.3 ) cDNA ORF clone, Drosophila erecta -> XP_001971572.1 Drosophila erecta ubiquitin-protein ligase E3B (LOC6544975), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODi03240
Clone ID Related Accession (Same CDS sequence)	XM_001971536.2 , XM_001971536.3 , XM_026977387.1
Accession Version	XM_001971536.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825198.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_001971536.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCCC AAAACCAAAA GACCAGCTTC CTGGAGCAGA CTAAGGCGGC GCGGGAGGAG 
CGGGCGCTGG AGAAGCGCCG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAAGGG 
TATGCGGCGC GTAGGAAGTA CCAGAAACGG ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCAGG ACGATAAGGA TACGGAACTG GAACTTGTGG CCAGCAACGT CTATCCGGTG 
CTCCGTCGCT ACCTCACCCA GATCAAGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGTCGTTA CATCATCAAG GCAATGGAGG CGGAGAGCAC CAAGCTATCC 
TATGCCGCCC TCTGTCTCTT CAAGGAACGC AGCCTGCCCT GGATCGCGCA CATTAAGATA 
CTGCTCACGA ACTGCCTAAC ATTGCTGCCG GAGCTGAAGC CGGAGAACCA TGCCGACAGC 
TTGTCCCTTG CCCTCTTCCT GCACACACTG GTGGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT CGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGCTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTC TACAAAATCA TGCGGCTCGT TTTGCTGCGC 
GGCACTGTGC GCGAGGAGTT GTCTGTGAAG CCCGTCACCC TGGTGGCCAT CATTACGCTA 
TGCCTTCGTC CCCTGATCGA TGGCAACTTT AGTCGAAATC TACTGGGCAA ATTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCACC AGAGTGTGCC GCAGTGCCTG 
GAACAGTTCT CATCTTTGGG GCTGCTTAAA AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGATG AATTCGGCAC GAGCATGCCG GGCACAAAAT CCCTGGCTTT TCTGGGAAAC 
ATTGTGAATT TGTTCAACAT TGATGGCCAA GGGGAGTCCA AGGAGCTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACCAAGGGA 
GTGTTCACTC AGTGGCACGA GCTGTTAGGA TGGCATACTC CGGGTCCAGA GCCTGCACAG 
AATCAGAATG TGGCCCTGAT TAAGAAGCAG TTTCACATGT TGTGGGATCA TCGCTGCATA 
AAGCTGTTGC TGGGAGATCT CTTGAAGCAA ATCAATATCA ACTACGAGCG AATCGAGTTC 
CAATCTCCCC AACAACCATC CACATCGAAT CTGCTGCGTC GGGCCCTGGA GCGTAGCTCC 
ACTAGAGGTG TTAACCTAAT GGGAGTAGCG AGCAGCAAGC AGGCTAAGCA GCAGTGGCGC 
AAGCTGGACA ATGCGGAGGT GGTGCAGGTG TCCCGAATCT GCGGCATGTA CTATGCCGCC 
CTCAACACCC TTTCCCAAAT GAAGCTGGAC ATCCTGACTG GCATTTGCTA CAACGACAAT 
GTGCTGTATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGCGG CATGAAGGAG 
TATCTGGAGC TGCTCAAATC CGAGTCAAAC ATGCAGAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAATACGA AATGTACACG 
GAGCAGAATC CCTTTAAACT TAACGACTAT GTGATGCTGA CCTACTTCCT AAATAATATT 
CTCTACAAAC TTATTAACGA TAATCTTCTG GCAGGTGCCA AGAACATTGT CCAGAATCCG 
GTTTTCCTCT CGCTGCACAC GCTGATGCTT TGCTTGTACC GCCGTGATTG CCGTCGCCCC 
TTCACGCCGC CCAACCACTG GCTGATACCG GAGGTCAAAC CCTCCACGTT CATCAACGAC 
CTGGAAAAGG CCAAGAGGAA CGCGATGCTG CTGCTCGCCA AGATGCCTCA CATTATACCG 
CACGAGGATC GGGTCAAGCT ATTCCGGAAG TTCGTGCAGA ATGAGAAGGC GGTGATGGGT 
CTGACGGAGA GTGCCTGCGC TTCTCCTCGC TCCGCACTGA TTGTCATCCA TCGCGATCGT 
ATTGTGGAGG ATGGATACCG GCAACTGGCC GCCCAGCCCA CGCAAGCCCT AAAAGGTGTT 
ATCCGCGTAC GTTTCATCAA TCAACAGGGA CTGCACGAGG CAGGAATCGA TCAGGATGGA 
GTGTTCAAGG AATTCCTTGA GGAGACCATC AAAAAGGTGT TCGACCCGTC GCTGAATCTC 
TTCAAAACCA CGTCGGATCA GCGGCTATAT CCCTCGCCCA TTTCCTACGT CCAGGACAAT 
CATTTGCAGC TCTTCGAGTT CGTGGGCCGC ATGCTGGGCA AGGCGGTGTA CGAAGGCATC 
GTGGTGGACG TCCCGTTTGC CTCCTTCTTC CTCTCCCAGC TTCTCGGCCA GACGCAACAG 
GCGCTGTACT CCTGCATGGA CGAGCTGCCG TCGCTGGACA ACGAGTTGTA TCGCAGTCTA 
ACCTTCATCA AACACTATAA GCAAGATGTG TCCGATCTGA ACCTTACCTT TTCCGTGGAC 
CAGGATGTGA TGGGCAAAAT TGTCACTCTG GCATTGCATC CGGGTGGCAA GTCGCGTGTG 
GTTAATGACC ACAACAAGCT GGTCTACATA CACTACATGG CATTTTTCCA CATGAACACC 
CAGATCCGCG AGCAGACGAT AGCCTTCAAT CGTGGCTTCC GCAGCATCGT CAATCCAGAA 
TGGTTGTCCC TTTTCTCGCC TCCGGAATTA CAGCGCCTGA TTTCTGGGGA CACCAGTCCA 
TTGGACTTGA AGGATCTGCG AAAGCATACA CACTATTACG GTGGCTTCCA CGACTCCCAT 
CGCGTGGTTG GCTGGCTCTG GGATATCCTG GCCAAGGATT TCACGGAGGA GGAGCGTAAG 
CTGTTCCTAA AGTTTGTTAC TAGCTGCTCA AAGCCTCCGC TTTTGGGTTT CGCCCACCTG 
GAGCCACCCT TCTCGATTCG TTGCGTGGAG GTGAGTGATG ACGAGGATAC GGGCGACACA 
ATCGGAAGTG TGATTCGCGG TTTCTTTACC ATACGTAAAA AGGATCCACT CAACCGCCTA 
CCCACCTCGT CGACCTGCTT CAACCTGCTG AAGCTGCCCA ACTACCAAAA GAAGTCCACG 
CTGAGGGACA AACTTCGCTA TGCTGTGAGC AGCAACACCG GATTCGAGCT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001971572.1
CDS	288..3524
Translation	MFAQNQKTSFLEQTKAAREERALEKRREQAAILLQSTLKGYAARRKYQKRIILDFDAMFTENQDDKDTELELVASNVYPVLRRYLTQIKLDKSDVRARERLERICRYIIKAMEAESTKLSYAALCLFKERSLPWIAHIKILLTNCLTLLPELKPENHADSLSLALFLHTLVVFTAPKSWAILRNAQFEKLQPAMQKICCNIQGHLVQHDFYKIMRLVLLRGTVREELSVKPVTLVAIITLCLRPLIDGNFSRNLLGKFLSEILSVPALIYHLHQSVPQCLEQFSSLGLLKKALSISGDVQWFDEFGTSMPGTKSLAFLGNIVNLFNIDGQGESKELAYPLLTETTTSLLELIPNTVTTKGVFTQWHELLGWHTPGPEPAQNQNVALIKKQFHMLWDHRCIKLLLGDLLKQININYERIEFQSPQQPSTSNLLRRALERSSTRGVNLMGVASSKQAKQQWRKLDNAEVVQVSRICGMYYAALNTLSQMKLDILTGICYNDNVLYDIWLLITSLGPNCGMKEYLELLKSESNMQKPQTAMLMLFCDCMTHYVTILDEYEMYTEQNPFKLNDYVMLTYFLNNILYKLINDNLLAGAKNIVQNPVFLSLHTLMLCLYRRDCRRPFTPPNHWLIPEVKPSTFINDLEKAKRNAMLLLAKMPHIIPHEDRVKLFRKFVQNEKAVMGLTESACASPRSALIVIHRDRIVEDGYRQLAAQPTQALKGVIRVRFINQQGLHEAGIDQDGVFKEFLEETIKKVFDPSLNLFKTTSDQRLYPSPISYVQDNHLQLFEFVGRMLGKAVYEGIVVDVPFASFFLSQLLGQTQQALYSCMDELPSLDNELYRSLTFIKHYKQDVSDLNLTFSVDQDVMGKIVTLALHPGGKSRVVNDHNKLVYIHYMAFFHMNTQIREQTIAFNRGFRSIVNPEWLSLFSPPELQRLISGDTSPLDLKDLRKHTHYYGGFHDSHRVVGWLWDILAKDFTEEERKLFLKFVTSCSKPPLLGFAHLEPPFSIRCVEVSDDEDTGDTIGSVIRGFFTIRKKDPLNRLPTSSTCFNLLKLPNYQKKSTLRDKLRYAVSSNTGFELS

Target ORF information:

RefSeq Version	XM_001971536.3
Organism	Drosophila erecta
Definition	Drosophila erecta ubiquitin-protein ligase E3B (LOC6544975), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001971536.3

ORF Insert Sequence:

ATGTTTGCCC AAAACCAAAA GACCAGCTTC CTGGAGCAGA CTAAGGCGGC GCGGGAGGAG 
CGGGCGCTGG AGAAGCGCCG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAAGGG 
TATGCGGCGC GTAGGAAGTA CCAGAAACGG ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCAGG ACGATAAGGA TACGGAACTG GAACTTGTGG CCAGCAACGT CTATCCGGTG 
CTCCGTCGCT ACCTCACCCA GATCAAGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGTCGTTA CATCATCAAG GCAATGGAGG CGGAGAGCAC CAAGCTATCC 
TATGCCGCCC TCTGTCTCTT CAAGGAACGC AGCCTGCCCT GGATCGCGCA CATTAAGATA 
CTGCTCACGA ACTGCCTAAC ATTGCTGCCG GAGCTGAAGC CGGAGAACCA TGCCGACAGC 
TTGTCCCTTG CCCTCTTCCT GCACACACTG GTGGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT CGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGCTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTC TACAAAATCA TGCGGCTCGT TTTGCTGCGC 
GGCACTGTGC GCGAGGAGTT GTCTGTGAAG CCCGTCACCC TGGTGGCCAT CATTACGCTA 
TGCCTTCGTC CCCTGATCGA TGGCAACTTT AGTCGAAATC TACTGGGCAA ATTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCACC AGAGTGTGCC GCAGTGCCTG 
GAACAGTTCT CATCTTTGGG GCTGCTTAAA AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGATG AATTCGGCAC GAGCATGCCG GGCACAAAAT CCCTGGCTTT TCTGGGAAAC 
ATTGTGAATT TGTTCAACAT TGATGGCCAA GGGGAGTCCA AGGAGCTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACCAAGGGA 
GTGTTCACTC AGTGGCACGA GCTGTTAGGA TGGCATACTC CGGGTCCAGA GCCTGCACAG 
AATCAGAATG TGGCCCTGAT TAAGAAGCAG TTTCACATGT TGTGGGATCA TCGCTGCATA 
AAGCTGTTGC TGGGAGATCT CTTGAAGCAA ATCAATATCA ACTACGAGCG AATCGAGTTC 
CAATCTCCCC AACAACCATC CACATCGAAT CTGCTGCGTC GGGCCCTGGA GCGTAGCTCC 
ACTAGAGGTG TTAACCTAAT GGGAGTAGCG AGCAGCAAGC AGGCTAAGCA GCAGTGGCGC 
AAGCTGGACA ATGCGGAGGT GGTGCAGGTG TCCCGAATCT GCGGCATGTA CTATGCCGCC 
CTCAACACCC TTTCCCAAAT GAAGCTGGAC ATCCTGACTG GCATTTGCTA CAACGACAAT 
GTGCTGTATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGCGG CATGAAGGAG 
TATCTGGAGC TGCTCAAATC CGAGTCAAAC ATGCAGAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAATACGA AATGTACACG 
GAGCAGAATC CCTTTAAACT TAACGACTAT GTGATGCTGA CCTACTTCCT AAATAATATT 
CTCTACAAAC TTATTAACGA TAATCTTCTG GCAGGTGCCA AGAACATTGT CCAGAATCCG 
GTTTTCCTCT CGCTGCACAC GCTGATGCTT TGCTTGTACC GCCGTGATTG CCGTCGCCCC 
TTCACGCCGC CCAACCACTG GCTGATACCG GAGGTCAAAC CCTCCACGTT CATCAACGAC 
CTGGAAAAGG CCAAGAGGAA CGCGATGCTG CTGCTCGCCA AGATGCCTCA CATTATACCG 
CACGAGGATC GGGTCAAGCT ATTCCGGAAG TTCGTGCAGA ATGAGAAGGC GGTGATGGGT 
CTGACGGAGA GTGCCTGCGC TTCTCCTCGC TCCGCACTGA TTGTCATCCA TCGCGATCGT 
ATTGTGGAGG ATGGATACCG GCAACTGGCC GCCCAGCCCA CGCAAGCCCT AAAAGGTGTT 
ATCCGCGTAC GTTTCATCAA TCAACAGGGA CTGCACGAGG CAGGAATCGA TCAGGATGGA 
GTGTTCAAGG AATTCCTTGA GGAGACCATC AAAAAGGTGT TCGACCCGTC GCTGAATCTC 
TTCAAAACCA CGTCGGATCA GCGGCTATAT CCCTCGCCCA TTTCCTACGT CCAGGACAAT 
CATTTGCAGC TCTTCGAGTT CGTGGGCCGC ATGCTGGGCA AGGCGGTGTA CGAAGGCATC 
GTGGTGGACG TCCCGTTTGC CTCCTTCTTC CTCTCCCAGC TTCTCGGCCA GACGCAACAG 
GCGCTGTACT CCTGCATGGA CGAGCTGCCG TCGCTGGACA ACGAGTTGTA TCGCAGTCTA 
ACCTTCATCA AACACTATAA GCAAGATGTG TCCGATCTGA ACCTTACCTT TTCCGTGGAC 
CAGGATGTGA TGGGCAAAAT TGTCACTCTG GCATTGCATC CGGGTGGCAA GTCGCGTGTG 
GTTAATGACC ACAACAAGCT GGTCTACATA CACTACATGG CATTTTTCCA CATGAACACC 
CAGATCCGCG AGCAGACGAT AGCCTTCAAT CGTGGCTTCC GCAGCATCGT CAATCCAGAA 
TGGTTGTCCC TTTTCTCGCC TCCGGAATTA CAGCGCCTGA TTTCTGGGGA CACCAGTCCA 
TTGGACTTGA AGGATCTGCG AAAGCATACA CACTATTACG GTGGCTTCCA CGACTCCCAT 
CGCGTGGTTG GCTGGCTCTG GGATATCCTG GCCAAGGATT TCACGGAGGA GGAGCGTAAG 
CTGTTCCTAA AGTTTGTTAC TAGCTGCTCA AAGCCTCCGC TTTTGGGTTT CGCCCACCTG 
GAGCCACCCT TCTCGATTCG TTGCGTGGAG GTGAGTGATG ACGAGGATAC GGGCGACACA 
ATCGGAAGTG TGATTCGCGG TTTCTTTACC ATACGTAAAA AGGATCCACT CAACCGCCTA 
CCCACCTCGT CGACCTGCTT CAACCTGCTG AAGCTGCCCA ACTACCAAAA GAAGTCCACG 
CTGAGGGACA AACTTCGCTA TGCTGTGAGC AGCAACACCG GATTCGAGCT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6544975 ( XM_026977387.1 ) cDNA ORF clone, Drosophila erecta -> XP_026833188.1 Drosophila erecta ubiquitin-protein ligase E3B (LOC6544975), transcript variant X2, mRNA.

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CloneID	ODi03240
Clone ID Related Accession (Same CDS sequence)	XM_001971536.2 , XM_001971536.3 , XM_026977387.1
Accession Version	XM_026977387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	ubiquitin-protein ligase E3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825198.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCCC AAAACCAAAA GACCAGCTTC CTGGAGCAGA CTAAGGCGGC GCGGGAGGAG 
CGGGCGCTGG AGAAGCGCCG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAAGGG 
TATGCGGCGC GTAGGAAGTA CCAGAAACGG ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCAGG ACGATAAGGA TACGGAACTG GAACTTGTGG CCAGCAACGT CTATCCGGTG 
CTCCGTCGCT ACCTCACCCA GATCAAGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGTCGTTA CATCATCAAG GCAATGGAGG CGGAGAGCAC CAAGCTATCC 
TATGCCGCCC TCTGTCTCTT CAAGGAACGC AGCCTGCCCT GGATCGCGCA CATTAAGATA 
CTGCTCACGA ACTGCCTAAC ATTGCTGCCG GAGCTGAAGC CGGAGAACCA TGCCGACAGC 
TTGTCCCTTG CCCTCTTCCT GCACACACTG GTGGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT CGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGCTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTC TACAAAATCA TGCGGCTCGT TTTGCTGCGC 
GGCACTGTGC GCGAGGAGTT GTCTGTGAAG CCCGTCACCC TGGTGGCCAT CATTACGCTA 
TGCCTTCGTC CCCTGATCGA TGGCAACTTT AGTCGAAATC TACTGGGCAA ATTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCACC AGAGTGTGCC GCAGTGCCTG 
GAACAGTTCT CATCTTTGGG GCTGCTTAAA AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGATG AATTCGGCAC GAGCATGCCG GGCACAAAAT CCCTGGCTTT TCTGGGAAAC 
ATTGTGAATT TGTTCAACAT TGATGGCCAA GGGGAGTCCA AGGAGCTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACCAAGGGA 
GTGTTCACTC AGTGGCACGA GCTGTTAGGA TGGCATACTC CGGGTCCAGA GCCTGCACAG 
AATCAGAATG TGGCCCTGAT TAAGAAGCAG TTTCACATGT TGTGGGATCA TCGCTGCATA 
AAGCTGTTGC TGGGAGATCT CTTGAAGCAA ATCAATATCA ACTACGAGCG AATCGAGTTC 
CAATCTCCCC AACAACCATC CACATCGAAT CTGCTGCGTC GGGCCCTGGA GCGTAGCTCC 
ACTAGAGGTG TTAACCTAAT GGGAGTAGCG AGCAGCAAGC AGGCTAAGCA GCAGTGGCGC 
AAGCTGGACA ATGCGGAGGT GGTGCAGGTG TCCCGAATCT GCGGCATGTA CTATGCCGCC 
CTCAACACCC TTTCCCAAAT GAAGCTGGAC ATCCTGACTG GCATTTGCTA CAACGACAAT 
GTGCTGTATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGCGG CATGAAGGAG 
TATCTGGAGC TGCTCAAATC CGAGTCAAAC ATGCAGAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAATACGA AATGTACACG 
GAGCAGAATC CCTTTAAACT TAACGACTAT GTGATGCTGA CCTACTTCCT AAATAATATT 
CTCTACAAAC TTATTAACGA TAATCTTCTG GCAGGTGCCA AGAACATTGT CCAGAATCCG 
GTTTTCCTCT CGCTGCACAC GCTGATGCTT TGCTTGTACC GCCGTGATTG CCGTCGCCCC 
TTCACGCCGC CCAACCACTG GCTGATACCG GAGGTCAAAC CCTCCACGTT CATCAACGAC 
CTGGAAAAGG CCAAGAGGAA CGCGATGCTG CTGCTCGCCA AGATGCCTCA CATTATACCG 
CACGAGGATC GGGTCAAGCT ATTCCGGAAG TTCGTGCAGA ATGAGAAGGC GGTGATGGGT 
CTGACGGAGA GTGCCTGCGC TTCTCCTCGC TCCGCACTGA TTGTCATCCA TCGCGATCGT 
ATTGTGGAGG ATGGATACCG GCAACTGGCC GCCCAGCCCA CGCAAGCCCT AAAAGGTGTT 
ATCCGCGTAC GTTTCATCAA TCAACAGGGA CTGCACGAGG CAGGAATCGA TCAGGATGGA 
GTGTTCAAGG AATTCCTTGA GGAGACCATC AAAAAGGTGT TCGACCCGTC GCTGAATCTC 
TTCAAAACCA CGTCGGATCA GCGGCTATAT CCCTCGCCCA TTTCCTACGT CCAGGACAAT 
CATTTGCAGC TCTTCGAGTT CGTGGGCCGC ATGCTGGGCA AGGCGGTGTA CGAAGGCATC 
GTGGTGGACG TCCCGTTTGC CTCCTTCTTC CTCTCCCAGC TTCTCGGCCA GACGCAACAG 
GCGCTGTACT CCTGCATGGA CGAGCTGCCG TCGCTGGACA ACGAGTTGTA TCGCAGTCTA 
ACCTTCATCA AACACTATAA GCAAGATGTG TCCGATCTGA ACCTTACCTT TTCCGTGGAC 
CAGGATGTGA TGGGCAAAAT TGTCACTCTG GCATTGCATC CGGGTGGCAA GTCGCGTGTG 
GTTAATGACC ACAACAAGCT GGTCTACATA CACTACATGG CATTTTTCCA CATGAACACC 
CAGATCCGCG AGCAGACGAT AGCCTTCAAT CGTGGCTTCC GCAGCATCGT CAATCCAGAA 
TGGTTGTCCC TTTTCTCGCC TCCGGAATTA CAGCGCCTGA TTTCTGGGGA CACCAGTCCA 
TTGGACTTGA AGGATCTGCG AAAGCATACA CACTATTACG GTGGCTTCCA CGACTCCCAT 
CGCGTGGTTG GCTGGCTCTG GGATATCCTG GCCAAGGATT TCACGGAGGA GGAGCGTAAG 
CTGTTCCTAA AGTTTGTTAC TAGCTGCTCA AAGCCTCCGC TTTTGGGTTT CGCCCACCTG 
GAGCCACCCT TCTCGATTCG TTGCGTGGAG GTGAGTGATG ACGAGGATAC GGGCGACACA 
ATCGGAAGTG TGATTCGCGG TTTCTTTACC ATACGTAAAA AGGATCCACT CAACCGCCTA 
CCCACCTCGT CGACCTGCTT CAACCTGCTG AAGCTGCCCA ACTACCAAAA GAAGTCCACG 
CTGAGGGACA AACTTCGCTA TGCTGTGAGC AGCAACACCG GATTCGAGCT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026833188.1
CDS	203..3439
Translation	MFAQNQKTSFLEQTKAAREERALEKRREQAAILLQSTLKGYAARRKYQKRIILDFDAMFTENQDDKDTELELVASNVYPVLRRYLTQIKLDKSDVRARERLERICRYIIKAMEAESTKLSYAALCLFKERSLPWIAHIKILLTNCLTLLPELKPENHADSLSLALFLHTLVVFTAPKSWAILRNAQFEKLQPAMQKICCNIQGHLVQHDFYKIMRLVLLRGTVREELSVKPVTLVAIITLCLRPLIDGNFSRNLLGKFLSEILSVPALIYHLHQSVPQCLEQFSSLGLLKKALSISGDVQWFDEFGTSMPGTKSLAFLGNIVNLFNIDGQGESKELAYPLLTETTTSLLELIPNTVTTKGVFTQWHELLGWHTPGPEPAQNQNVALIKKQFHMLWDHRCIKLLLGDLLKQININYERIEFQSPQQPSTSNLLRRALERSSTRGVNLMGVASSKQAKQQWRKLDNAEVVQVSRICGMYYAALNTLSQMKLDILTGICYNDNVLYDIWLLITSLGPNCGMKEYLELLKSESNMQKPQTAMLMLFCDCMTHYVTILDEYEMYTEQNPFKLNDYVMLTYFLNNILYKLINDNLLAGAKNIVQNPVFLSLHTLMLCLYRRDCRRPFTPPNHWLIPEVKPSTFINDLEKAKRNAMLLLAKMPHIIPHEDRVKLFRKFVQNEKAVMGLTESACASPRSALIVIHRDRIVEDGYRQLAAQPTQALKGVIRVRFINQQGLHEAGIDQDGVFKEFLEETIKKVFDPSLNLFKTTSDQRLYPSPISYVQDNHLQLFEFVGRMLGKAVYEGIVVDVPFASFFLSQLLGQTQQALYSCMDELPSLDNELYRSLTFIKHYKQDVSDLNLTFSVDQDVMGKIVTLALHPGGKSRVVNDHNKLVYIHYMAFFHMNTQIREQTIAFNRGFRSIVNPEWLSLFSPPELQRLISGDTSPLDLKDLRKHTHYYGGFHDSHRVVGWLWDILAKDFTEEERKLFLKFVTSCSKPPLLGFAHLEPPFSIRCVEVSDDEDTGDTIGSVIRGFFTIRKKDPLNRLPTSSTCFNLLKLPNYQKKSTLRDKLRYAVSSNTGFELS

Target ORF information:

RefSeq Version	XM_026977387.1
Organism	Drosophila erecta
Definition	Drosophila erecta ubiquitin-protein ligase E3B (LOC6544975), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026977387.1

ORF Insert Sequence:

ATGTTTGCCC AAAACCAAAA GACCAGCTTC CTGGAGCAGA CTAAGGCGGC GCGGGAGGAG 
CGGGCGCTGG AGAAGCGCCG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAAGGG 
TATGCGGCGC GTAGGAAGTA CCAGAAACGG ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCAGG ACGATAAGGA TACGGAACTG GAACTTGTGG CCAGCAACGT CTATCCGGTG 
CTCCGTCGCT ACCTCACCCA GATCAAGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGTCGTTA CATCATCAAG GCAATGGAGG CGGAGAGCAC CAAGCTATCC 
TATGCCGCCC TCTGTCTCTT CAAGGAACGC AGCCTGCCCT GGATCGCGCA CATTAAGATA 
CTGCTCACGA ACTGCCTAAC ATTGCTGCCG GAGCTGAAGC CGGAGAACCA TGCCGACAGC 
TTGTCCCTTG CCCTCTTCCT GCACACACTG GTGGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT CGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGCTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTC TACAAAATCA TGCGGCTCGT TTTGCTGCGC 
GGCACTGTGC GCGAGGAGTT GTCTGTGAAG CCCGTCACCC TGGTGGCCAT CATTACGCTA 
TGCCTTCGTC CCCTGATCGA TGGCAACTTT AGTCGAAATC TACTGGGCAA ATTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCACC AGAGTGTGCC GCAGTGCCTG 
GAACAGTTCT CATCTTTGGG GCTGCTTAAA AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGATG AATTCGGCAC GAGCATGCCG GGCACAAAAT CCCTGGCTTT TCTGGGAAAC 
ATTGTGAATT TGTTCAACAT TGATGGCCAA GGGGAGTCCA AGGAGCTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACCAAGGGA 
GTGTTCACTC AGTGGCACGA GCTGTTAGGA TGGCATACTC CGGGTCCAGA GCCTGCACAG 
AATCAGAATG TGGCCCTGAT TAAGAAGCAG TTTCACATGT TGTGGGATCA TCGCTGCATA 
AAGCTGTTGC TGGGAGATCT CTTGAAGCAA ATCAATATCA ACTACGAGCG AATCGAGTTC 
CAATCTCCCC AACAACCATC CACATCGAAT CTGCTGCGTC GGGCCCTGGA GCGTAGCTCC 
ACTAGAGGTG TTAACCTAAT GGGAGTAGCG AGCAGCAAGC AGGCTAAGCA GCAGTGGCGC 
AAGCTGGACA ATGCGGAGGT GGTGCAGGTG TCCCGAATCT GCGGCATGTA CTATGCCGCC 
CTCAACACCC TTTCCCAAAT GAAGCTGGAC ATCCTGACTG GCATTTGCTA CAACGACAAT 
GTGCTGTATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGCGG CATGAAGGAG 
TATCTGGAGC TGCTCAAATC CGAGTCAAAC ATGCAGAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAATACGA AATGTACACG 
GAGCAGAATC CCTTTAAACT TAACGACTAT GTGATGCTGA CCTACTTCCT AAATAATATT 
CTCTACAAAC TTATTAACGA TAATCTTCTG GCAGGTGCCA AGAACATTGT CCAGAATCCG 
GTTTTCCTCT CGCTGCACAC GCTGATGCTT TGCTTGTACC GCCGTGATTG CCGTCGCCCC 
TTCACGCCGC CCAACCACTG GCTGATACCG GAGGTCAAAC CCTCCACGTT CATCAACGAC 
CTGGAAAAGG CCAAGAGGAA CGCGATGCTG CTGCTCGCCA AGATGCCTCA CATTATACCG 
CACGAGGATC GGGTCAAGCT ATTCCGGAAG TTCGTGCAGA ATGAGAAGGC GGTGATGGGT 
CTGACGGAGA GTGCCTGCGC TTCTCCTCGC TCCGCACTGA TTGTCATCCA TCGCGATCGT 
ATTGTGGAGG ATGGATACCG GCAACTGGCC GCCCAGCCCA CGCAAGCCCT AAAAGGTGTT 
ATCCGCGTAC GTTTCATCAA TCAACAGGGA CTGCACGAGG CAGGAATCGA TCAGGATGGA 
GTGTTCAAGG AATTCCTTGA GGAGACCATC AAAAAGGTGT TCGACCCGTC GCTGAATCTC 
TTCAAAACCA CGTCGGATCA GCGGCTATAT CCCTCGCCCA TTTCCTACGT CCAGGACAAT 
CATTTGCAGC TCTTCGAGTT CGTGGGCCGC ATGCTGGGCA AGGCGGTGTA CGAAGGCATC 
GTGGTGGACG TCCCGTTTGC CTCCTTCTTC CTCTCCCAGC TTCTCGGCCA GACGCAACAG 
GCGCTGTACT CCTGCATGGA CGAGCTGCCG TCGCTGGACA ACGAGTTGTA TCGCAGTCTA 
ACCTTCATCA AACACTATAA GCAAGATGTG TCCGATCTGA ACCTTACCTT TTCCGTGGAC 
CAGGATGTGA TGGGCAAAAT TGTCACTCTG GCATTGCATC CGGGTGGCAA GTCGCGTGTG 
GTTAATGACC ACAACAAGCT GGTCTACATA CACTACATGG CATTTTTCCA CATGAACACC 
CAGATCCGCG AGCAGACGAT AGCCTTCAAT CGTGGCTTCC GCAGCATCGT CAATCCAGAA 
TGGTTGTCCC TTTTCTCGCC TCCGGAATTA CAGCGCCTGA TTTCTGGGGA CACCAGTCCA 
TTGGACTTGA AGGATCTGCG AAAGCATACA CACTATTACG GTGGCTTCCA CGACTCCCAT 
CGCGTGGTTG GCTGGCTCTG GGATATCCTG GCCAAGGATT TCACGGAGGA GGAGCGTAAG 
CTGTTCCTAA AGTTTGTTAC TAGCTGCTCA AAGCCTCCGC TTTTGGGTTT CGCCCACCTG 
GAGCCACCCT TCTCGATTCG TTGCGTGGAG GTGAGTGATG ACGAGGATAC GGGCGACACA 
ATCGGAAGTG TGATTCGCGG TTTCTTTACC ATACGTAAAA AGGATCCACT CAACCGCCTA 
CCCACCTCGT CGACCTGCTT CAACCTGCTG AAGCTGCCCA ACTACCAAAA GAAGTCCACG 
CTGAGGGACA AACTTCGCTA TGCTGTGAGC AGCAACACCG GATTCGAGCT GTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6544975 ( XM_015157920.1 ) cDNA ORF clone, Drosophila erecta -> XP_015013406.1 Drosophila erecta uncharacterized protein, transcript variant B (Dere\GG14367), mRNA.

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CloneID	ODi38167
Clone ID Related Accession (Same CDS sequence)	XM_015157920.1 , XM_015157920.2
Accession Version	XM_015157920.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956549). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGCCC AAAACCAAAA GACCAGCTTC CTGGAGCAGA CTAAGGCGGC GCGGGAGGAG 
CGGGCGCTGG AGAAGCGCCG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAAGGG 
TATGCGGCGC GTAGGAAGTA CCAGAAACGG ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCAGG ACGATAAGGA TACGGAACTG GAACTTGTGG CCAGCAACGT CTATCCGGTG 
CTCCGTCGCT ACCTCACCCA GATCAAGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGTCGTTA CATCATCAAG GCAATGGAGG CGGAGAGCAC CAAGCTATCC 
TATGCCGCCC TCTGTCTCTT CAAGGAACGC AGCCTGCCCT GGATCGCGCA CATTAAGATA 
CTGCTCACGA ACTGCCTAAC ATTGCTGCCG GAGCTGAAGC CGGAGAACCA TGCCGACAGC 
TTGTCCCTTG CCCTCTTCCT GCACACACTG GTGGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT CGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGCTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTC TACAAAATCA TGCGGCTCGT TTTGCTGCGC 
GGCACTGTGC GCGAGGAGTT GTCTGTGAAG CCCGTCACCC TGGTGGCCAT CATTACGCTA 
TGCCTTCGTC CCCTGATCGA TGGCAACTTT AGTCGAAATC TACTGGGCAA ATTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCACC AGAGTGTGCC GCAGTGCCTG 
GAACAGTTCT CATCTTTGGG GCTGCTTAAA AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGATG AATTCGGCAC GAGCATGCCG GGCACAAAAT CCCTGGCTTT TCTGGGAAAC 
ATTGTGAATT TGTTCAACAT TGATGGCCAA GGGGAGTCCA AGGAGCTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACCAAGGGA 
GTGTTCACTC AGTGGCACGA GCTGTTAGGA TGGCATACTC CGGGTCCAGA GCCTGCACAG 
AATCAGAATG TGGCCCTGAT TAAGAAGCAG TTTCACATGT TGTGGGATCA TCGCTGCATA 
AAGCTGTTGC TGGGAGATCT CTTGAAGCAA ATCAATATCA ACTACGAGCG AATCGAGTTC 
CAATCTCCCC AACAACCATC CACATCGAAT CTGCTGCGTC GGGCCCTGGA GCGTAGCTCC 
ACTAGAGGTG TTAACCTAAT GGGAGTAGCG AGCAGCAAGC AGGCTAAGCA GCAGTGGCGC 
AAGCTGGACA ATGCGGAGGT GGTGCAGGTG TCCCGAATCT GCGGCATGTA CTATGCCGCC 
CTCAACACCC TTTCCCAAAT GAAGCTGGAC ATCCTGACTG GCATTTGCTA CAACGACAAT 
GTGCTGTATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGCGG CATGAAGGAG 
TATCTGGAGC TGCTCAAATC CGAGTCAAAC ATGCAGAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAATACGA AATGTACACG 
GAGCAGAATC CCTTTAAACT TAACGACTAT GTGATGCTGA CCTACTTCCT AAATAATATT 
CTCTACAAAC TTATTAACGA TAATCTTCTG GGTGCCAAGA ACATTGTCCA GAATCCGGTT 
TTCCTCTCGC TGCACACGCT GATGCTTTGC TTGTACCGCC GTGATTGCCG TCGCCCCTTC 
ACGCCGCCCA ACCACTGGCT GATACCGGAG GTCAAACCCT CCACGTTCAT CAACGACCTG 
GAAAAGGCCA AGAGGAACGC GATGCTGCTG CTCGCCAAGA TGCCTCACAT TATACCGCAC 
GAGGATCGGG TCAAGCTATT CCGGAAGTTC GTGCAGAATG AGAAGGCGGT GATGGGTCTG 
ACGGAGAGTG CCTGCGCTTC TCCTCGCTCC GCACTGATTG TCATCCATCG CGATCGTATT 
GTGGAGGATG GATACCGGCA ACTGGCCGCC CAGCCCACGC AAGCCCTAAA AGGTGTTATC 
CGCGTACGTT TCATCAATCA ACAGGGACTG CACGAGGCAG GAATCGATCA GGATGGAGTG 
TTCAAGGAAT TCCTTGAGGA GACCATCAAA AAGGTGTTCG ACCCGTCGCT GAATCTCTTC 
AAAACCACGT CGGATCAGCG GCTATATCCC TCGCCCATTT CCTACGTCCA GGACAATCAT 
TTGCAGCTCT TCGAGTTCGT GGGCCGCATG CTGGGCAAGG CGGTGTACGA AGGCATCGTG 
GTGGACGTCC CGTTTGCCTC CTTCTTCCTC TCCCAGCTTC TCGGCCAGAC GCAACAGGCG 
CTGTACTCCT GCATGGACGA GCTGCCGTCG CTGGACAACG AGTTGTATCG CAGTCTAACC 
TTCATCAAAC ACTATAAGCA AGATGTGTCC GATCTGAACC TTACCTTTTC CGTGGACCAG 
GATGTGATGG GCAAAATTGT CACTCTGGCA TTGCATCCGG GTGGCAAGTC GCGTGTGGTT 
AATGACCACA ACAAGCTGGT CTACATACAC TACATGGCAT TTTTCCACAT GAACACCCAG 
ATCCGCGAGC AGACGATAGC CTTCAATCGT GGCTTCCGCA GCATCGTCAA TCCAGAATGG 
TTGTCCCTTT TCTCGCCTCC GGAATTACAG CGCCTGATTT CTGGGGACAC CAGTCCATTG 
GACTTGAAGG ATCTGCGAAA GCATACACAC TATTACGGTG GCTTCCACGA CTCCCATCGC 
GTGGTTGGCT GGCTCTGGGA TATCCTGGCC AAGGATTTCA CGGAGGAGGA GCGTAAGCTG 
TTCCTAAAGT TTGTTACTAG CTGCTCAAAG CCTCCGCTTT TGGGTTTCGC CCACCTGGAG 
CCACCCTTCT CGATTCGTTG CGTGGAGGTG AGTGATGACG AGGATACGGG CGACACAATC 
GGAAGTGTGA TTCGCGGTTT CTTTACCATA CGTAAAAAGG ATCCACTCAA CCGCCTACCC 
ACCTCGTCGA CCTGCTTCAA CCTGCTGAAG CTGCCCAACT ACCAAAAGAA GTCCACGCTG 
AGGGACAAAC TTCGCTATGC TGTGAGCAGC AACACCGGAT TCGAGCTGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015013406.1
CDS	272..3505
Translation	MFAQNQKTSFLEQTKAAREERALEKRREQAAILLQSTLKGYAARRKYQKRIILDFDAMFTENQDDKDTELELVASNVYPVLRRYLTQIKLDKSDVRARERLERICRYIIKAMEAESTKLSYAALCLFKERSLPWIAHIKILLTNCLTLLPELKPENHADSLSLALFLHTLVVFTAPKSWAILRNAQFEKLQPAMQKICCNIQGHLVQHDFYKIMRLVLLRGTVREELSVKPVTLVAIITLCLRPLIDGNFSRNLLGKFLSEILSVPALIYHLHQSVPQCLEQFSSLGLLKKALSISGDVQWFDEFGTSMPGTKSLAFLGNIVNLFNIDGQGESKELAYPLLTETTTSLLELIPNTVTTKGVFTQWHELLGWHTPGPEPAQNQNVALIKKQFHMLWDHRCIKLLLGDLLKQININYERIEFQSPQQPSTSNLLRRALERSSTRGVNLMGVASSKQAKQQWRKLDNAEVVQVSRICGMYYAALNTLSQMKLDILTGICYNDNVLYDIWLLITSLGPNCGMKEYLELLKSESNMQKPQTAMLMLFCDCMTHYVTILDEYEMYTEQNPFKLNDYVMLTYFLNNILYKLINDNLLGAKNIVQNPVFLSLHTLMLCLYRRDCRRPFTPPNHWLIPEVKPSTFINDLEKAKRNAMLLLAKMPHIIPHEDRVKLFRKFVQNEKAVMGLTESACASPRSALIVIHRDRIVEDGYRQLAAQPTQALKGVIRVRFINQQGLHEAGIDQDGVFKEFLEETIKKVFDPSLNLFKTTSDQRLYPSPISYVQDNHLQLFEFVGRMLGKAVYEGIVVDVPFASFFLSQLLGQTQQALYSCMDELPSLDNELYRSLTFIKHYKQDVSDLNLTFSVDQDVMGKIVTLALHPGGKSRVVNDHNKLVYIHYMAFFHMNTQIREQTIAFNRGFRSIVNPEWLSLFSPPELQRLISGDTSPLDLKDLRKHTHYYGGFHDSHRVVGWLWDILAKDFTEEERKLFLKFVTSCSKPPLLGFAHLEPPFSIRCVEVSDDEDTGDTIGSVIRGFFTIRKKDPLNRLPTSSTCFNLLKLPNYQKKSTLRDKLRYAVSSNTGFELS

Target ORF information:

RefSeq Version	XM_015157920.1
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein, transcript variant B (Dere\GG14367), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157920.1

ORF Insert Sequence:

ATGTTTGCCC AAAACCAAAA GACCAGCTTC CTGGAGCAGA CTAAGGCGGC GCGGGAGGAG 
CGGGCGCTGG AGAAGCGCCG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAAGGG 
TATGCGGCGC GTAGGAAGTA CCAGAAACGG ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCAGG ACGATAAGGA TACGGAACTG GAACTTGTGG CCAGCAACGT CTATCCGGTG 
CTCCGTCGCT ACCTCACCCA GATCAAGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGTCGTTA CATCATCAAG GCAATGGAGG CGGAGAGCAC CAAGCTATCC 
TATGCCGCCC TCTGTCTCTT CAAGGAACGC AGCCTGCCCT GGATCGCGCA CATTAAGATA 
CTGCTCACGA ACTGCCTAAC ATTGCTGCCG GAGCTGAAGC CGGAGAACCA TGCCGACAGC 
TTGTCCCTTG CCCTCTTCCT GCACACACTG GTGGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT CGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGCTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTC TACAAAATCA TGCGGCTCGT TTTGCTGCGC 
GGCACTGTGC GCGAGGAGTT GTCTGTGAAG CCCGTCACCC TGGTGGCCAT CATTACGCTA 
TGCCTTCGTC CCCTGATCGA TGGCAACTTT AGTCGAAATC TACTGGGCAA ATTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCACC AGAGTGTGCC GCAGTGCCTG 
GAACAGTTCT CATCTTTGGG GCTGCTTAAA AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGATG AATTCGGCAC GAGCATGCCG GGCACAAAAT CCCTGGCTTT TCTGGGAAAC 
ATTGTGAATT TGTTCAACAT TGATGGCCAA GGGGAGTCCA AGGAGCTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACCAAGGGA 
GTGTTCACTC AGTGGCACGA GCTGTTAGGA TGGCATACTC CGGGTCCAGA GCCTGCACAG 
AATCAGAATG TGGCCCTGAT TAAGAAGCAG TTTCACATGT TGTGGGATCA TCGCTGCATA 
AAGCTGTTGC TGGGAGATCT CTTGAAGCAA ATCAATATCA ACTACGAGCG AATCGAGTTC 
CAATCTCCCC AACAACCATC CACATCGAAT CTGCTGCGTC GGGCCCTGGA GCGTAGCTCC 
ACTAGAGGTG TTAACCTAAT GGGAGTAGCG AGCAGCAAGC AGGCTAAGCA GCAGTGGCGC 
AAGCTGGACA ATGCGGAGGT GGTGCAGGTG TCCCGAATCT GCGGCATGTA CTATGCCGCC 
CTCAACACCC TTTCCCAAAT GAAGCTGGAC ATCCTGACTG GCATTTGCTA CAACGACAAT 
GTGCTGTATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGCGG CATGAAGGAG 
TATCTGGAGC TGCTCAAATC CGAGTCAAAC ATGCAGAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAATACGA AATGTACACG 
GAGCAGAATC CCTTTAAACT TAACGACTAT GTGATGCTGA CCTACTTCCT AAATAATATT 
CTCTACAAAC TTATTAACGA TAATCTTCTG GGTGCCAAGA ACATTGTCCA GAATCCGGTT 
TTCCTCTCGC TGCACACGCT GATGCTTTGC TTGTACCGCC GTGATTGCCG TCGCCCCTTC 
ACGCCGCCCA ACCACTGGCT GATACCGGAG GTCAAACCCT CCACGTTCAT CAACGACCTG 
GAAAAGGCCA AGAGGAACGC GATGCTGCTG CTCGCCAAGA TGCCTCACAT TATACCGCAC 
GAGGATCGGG TCAAGCTATT CCGGAAGTTC GTGCAGAATG AGAAGGCGGT GATGGGTCTG 
ACGGAGAGTG CCTGCGCTTC TCCTCGCTCC GCACTGATTG TCATCCATCG CGATCGTATT 
GTGGAGGATG GATACCGGCA ACTGGCCGCC CAGCCCACGC AAGCCCTAAA AGGTGTTATC 
CGCGTACGTT TCATCAATCA ACAGGGACTG CACGAGGCAG GAATCGATCA GGATGGAGTG 
TTCAAGGAAT TCCTTGAGGA GACCATCAAA AAGGTGTTCG ACCCGTCGCT GAATCTCTTC 
AAAACCACGT CGGATCAGCG GCTATATCCC TCGCCCATTT CCTACGTCCA GGACAATCAT 
TTGCAGCTCT TCGAGTTCGT GGGCCGCATG CTGGGCAAGG CGGTGTACGA AGGCATCGTG 
GTGGACGTCC CGTTTGCCTC CTTCTTCCTC TCCCAGCTTC TCGGCCAGAC GCAACAGGCG 
CTGTACTCCT GCATGGACGA GCTGCCGTCG CTGGACAACG AGTTGTATCG CAGTCTAACC 
TTCATCAAAC ACTATAAGCA AGATGTGTCC GATCTGAACC TTACCTTTTC CGTGGACCAG 
GATGTGATGG GCAAAATTGT CACTCTGGCA TTGCATCCGG GTGGCAAGTC GCGTGTGGTT 
AATGACCACA ACAAGCTGGT CTACATACAC TACATGGCAT TTTTCCACAT GAACACCCAG 
ATCCGCGAGC AGACGATAGC CTTCAATCGT GGCTTCCGCA GCATCGTCAA TCCAGAATGG 
TTGTCCCTTT TCTCGCCTCC GGAATTACAG CGCCTGATTT CTGGGGACAC CAGTCCATTG 
GACTTGAAGG ATCTGCGAAA GCATACACAC TATTACGGTG GCTTCCACGA CTCCCATCGC 
GTGGTTGGCT GGCTCTGGGA TATCCTGGCC AAGGATTTCA CGGAGGAGGA GCGTAAGCTG 
TTCCTAAAGT TTGTTACTAG CTGCTCAAAG CCTCCGCTTT TGGGTTTCGC CCACCTGGAG 
CCACCCTTCT CGATTCGTTG CGTGGAGGTG AGTGATGACG AGGATACGGG CGACACAATC 
GGAAGTGTGA TTCGCGGTTT CTTTACCATA CGTAAAAAGG ATCCACTCAA CCGCCTACCC 
ACCTCGTCGA CCTGCTTCAA CCTGCTGAAG CTGCCCAACT ACCAAAAGAA GTCCACGCTG 
AGGGACAAAC TTCGCTATGC TGTGAGCAGC AACACCGGAT TCGAGCTGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6544975 ( XM_015157920.2 ) cDNA ORF clone, Drosophila erecta -> XP_015013406.1 Drosophila erecta ubiquitin-protein ligase E3B (LOC6544975), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODi38167
Clone ID Related Accession (Same CDS sequence)	XM_015157920.1 , XM_015157920.2
Accession Version	XM_015157920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	ubiquitin-protein ligase E3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825198.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_015157920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCCC AAAACCAAAA GACCAGCTTC CTGGAGCAGA CTAAGGCGGC GCGGGAGGAG 
CGGGCGCTGG AGAAGCGCCG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAAGGG 
TATGCGGCGC GTAGGAAGTA CCAGAAACGG ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCAGG ACGATAAGGA TACGGAACTG GAACTTGTGG CCAGCAACGT CTATCCGGTG 
CTCCGTCGCT ACCTCACCCA GATCAAGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGTCGTTA CATCATCAAG GCAATGGAGG CGGAGAGCAC CAAGCTATCC 
TATGCCGCCC TCTGTCTCTT CAAGGAACGC AGCCTGCCCT GGATCGCGCA CATTAAGATA 
CTGCTCACGA ACTGCCTAAC ATTGCTGCCG GAGCTGAAGC CGGAGAACCA TGCCGACAGC 
TTGTCCCTTG CCCTCTTCCT GCACACACTG GTGGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT CGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGCTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTC TACAAAATCA TGCGGCTCGT TTTGCTGCGC 
GGCACTGTGC GCGAGGAGTT GTCTGTGAAG CCCGTCACCC TGGTGGCCAT CATTACGCTA 
TGCCTTCGTC CCCTGATCGA TGGCAACTTT AGTCGAAATC TACTGGGCAA ATTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCACC AGAGTGTGCC GCAGTGCCTG 
GAACAGTTCT CATCTTTGGG GCTGCTTAAA AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGATG AATTCGGCAC GAGCATGCCG GGCACAAAAT CCCTGGCTTT TCTGGGAAAC 
ATTGTGAATT TGTTCAACAT TGATGGCCAA GGGGAGTCCA AGGAGCTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACCAAGGGA 
GTGTTCACTC AGTGGCACGA GCTGTTAGGA TGGCATACTC CGGGTCCAGA GCCTGCACAG 
AATCAGAATG TGGCCCTGAT TAAGAAGCAG TTTCACATGT TGTGGGATCA TCGCTGCATA 
AAGCTGTTGC TGGGAGATCT CTTGAAGCAA ATCAATATCA ACTACGAGCG AATCGAGTTC 
CAATCTCCCC AACAACCATC CACATCGAAT CTGCTGCGTC GGGCCCTGGA GCGTAGCTCC 
ACTAGAGGTG TTAACCTAAT GGGAGTAGCG AGCAGCAAGC AGGCTAAGCA GCAGTGGCGC 
AAGCTGGACA ATGCGGAGGT GGTGCAGGTG TCCCGAATCT GCGGCATGTA CTATGCCGCC 
CTCAACACCC TTTCCCAAAT GAAGCTGGAC ATCCTGACTG GCATTTGCTA CAACGACAAT 
GTGCTGTATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGCGG CATGAAGGAG 
TATCTGGAGC TGCTCAAATC CGAGTCAAAC ATGCAGAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAATACGA AATGTACACG 
GAGCAGAATC CCTTTAAACT TAACGACTAT GTGATGCTGA CCTACTTCCT AAATAATATT 
CTCTACAAAC TTATTAACGA TAATCTTCTG GGTGCCAAGA ACATTGTCCA GAATCCGGTT 
TTCCTCTCGC TGCACACGCT GATGCTTTGC TTGTACCGCC GTGATTGCCG TCGCCCCTTC 
ACGCCGCCCA ACCACTGGCT GATACCGGAG GTCAAACCCT CCACGTTCAT CAACGACCTG 
GAAAAGGCCA AGAGGAACGC GATGCTGCTG CTCGCCAAGA TGCCTCACAT TATACCGCAC 
GAGGATCGGG TCAAGCTATT CCGGAAGTTC GTGCAGAATG AGAAGGCGGT GATGGGTCTG 
ACGGAGAGTG CCTGCGCTTC TCCTCGCTCC GCACTGATTG TCATCCATCG CGATCGTATT 
GTGGAGGATG GATACCGGCA ACTGGCCGCC CAGCCCACGC AAGCCCTAAA AGGTGTTATC 
CGCGTACGTT TCATCAATCA ACAGGGACTG CACGAGGCAG GAATCGATCA GGATGGAGTG 
TTCAAGGAAT TCCTTGAGGA GACCATCAAA AAGGTGTTCG ACCCGTCGCT GAATCTCTTC 
AAAACCACGT CGGATCAGCG GCTATATCCC TCGCCCATTT CCTACGTCCA GGACAATCAT 
TTGCAGCTCT TCGAGTTCGT GGGCCGCATG CTGGGCAAGG CGGTGTACGA AGGCATCGTG 
GTGGACGTCC CGTTTGCCTC CTTCTTCCTC TCCCAGCTTC TCGGCCAGAC GCAACAGGCG 
CTGTACTCCT GCATGGACGA GCTGCCGTCG CTGGACAACG AGTTGTATCG CAGTCTAACC 
TTCATCAAAC ACTATAAGCA AGATGTGTCC GATCTGAACC TTACCTTTTC CGTGGACCAG 
GATGTGATGG GCAAAATTGT CACTCTGGCA TTGCATCCGG GTGGCAAGTC GCGTGTGGTT 
AATGACCACA ACAAGCTGGT CTACATACAC TACATGGCAT TTTTCCACAT GAACACCCAG 
ATCCGCGAGC AGACGATAGC CTTCAATCGT GGCTTCCGCA GCATCGTCAA TCCAGAATGG 
TTGTCCCTTT TCTCGCCTCC GGAATTACAG CGCCTGATTT CTGGGGACAC CAGTCCATTG 
GACTTGAAGG ATCTGCGAAA GCATACACAC TATTACGGTG GCTTCCACGA CTCCCATCGC 
GTGGTTGGCT GGCTCTGGGA TATCCTGGCC AAGGATTTCA CGGAGGAGGA GCGTAAGCTG 
TTCCTAAAGT TTGTTACTAG CTGCTCAAAG CCTCCGCTTT TGGGTTTCGC CCACCTGGAG 
CCACCCTTCT CGATTCGTTG CGTGGAGGTG AGTGATGACG AGGATACGGG CGACACAATC 
GGAAGTGTGA TTCGCGGTTT CTTTACCATA CGTAAAAAGG ATCCACTCAA CCGCCTACCC 
ACCTCGTCGA CCTGCTTCAA CCTGCTGAAG CTGCCCAACT ACCAAAAGAA GTCCACGCTG 
AGGGACAAAC TTCGCTATGC TGTGAGCAGC AACACCGGAT TCGAGCTGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015013406.1
CDS	288..3521
Translation	MFAQNQKTSFLEQTKAAREERALEKRREQAAILLQSTLKGYAARRKYQKRIILDFDAMFTENQDDKDTELELVASNVYPVLRRYLTQIKLDKSDVRARERLERICRYIIKAMEAESTKLSYAALCLFKERSLPWIAHIKILLTNCLTLLPELKPENHADSLSLALFLHTLVVFTAPKSWAILRNAQFEKLQPAMQKICCNIQGHLVQHDFYKIMRLVLLRGTVREELSVKPVTLVAIITLCLRPLIDGNFSRNLLGKFLSEILSVPALIYHLHQSVPQCLEQFSSLGLLKKALSISGDVQWFDEFGTSMPGTKSLAFLGNIVNLFNIDGQGESKELAYPLLTETTTSLLELIPNTVTTKGVFTQWHELLGWHTPGPEPAQNQNVALIKKQFHMLWDHRCIKLLLGDLLKQININYERIEFQSPQQPSTSNLLRRALERSSTRGVNLMGVASSKQAKQQWRKLDNAEVVQVSRICGMYYAALNTLSQMKLDILTGICYNDNVLYDIWLLITSLGPNCGMKEYLELLKSESNMQKPQTAMLMLFCDCMTHYVTILDEYEMYTEQNPFKLNDYVMLTYFLNNILYKLINDNLLGAKNIVQNPVFLSLHTLMLCLYRRDCRRPFTPPNHWLIPEVKPSTFINDLEKAKRNAMLLLAKMPHIIPHEDRVKLFRKFVQNEKAVMGLTESACASPRSALIVIHRDRIVEDGYRQLAAQPTQALKGVIRVRFINQQGLHEAGIDQDGVFKEFLEETIKKVFDPSLNLFKTTSDQRLYPSPISYVQDNHLQLFEFVGRMLGKAVYEGIVVDVPFASFFLSQLLGQTQQALYSCMDELPSLDNELYRSLTFIKHYKQDVSDLNLTFSVDQDVMGKIVTLALHPGGKSRVVNDHNKLVYIHYMAFFHMNTQIREQTIAFNRGFRSIVNPEWLSLFSPPELQRLISGDTSPLDLKDLRKHTHYYGGFHDSHRVVGWLWDILAKDFTEEERKLFLKFVTSCSKPPLLGFAHLEPPFSIRCVEVSDDEDTGDTIGSVIRGFFTIRKKDPLNRLPTSSTCFNLLKLPNYQKKSTLRDKLRYAVSSNTGFELS

Target ORF information:

RefSeq Version	XM_015157920.2
Organism	Drosophila erecta
Definition	Drosophila erecta ubiquitin-protein ligase E3B (LOC6544975), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015157920.2

ORF Insert Sequence:

ATGTTTGCCC AAAACCAAAA GACCAGCTTC CTGGAGCAGA CTAAGGCGGC GCGGGAGGAG 
CGGGCGCTGG AGAAGCGCCG CGAACAGGCG GCCATCCTGC TGCAGTCCAC GCTCAAAGGG 
TATGCGGCGC GTAGGAAGTA CCAGAAACGG ATCATACTCG ATTTCGATGC CATGTTCACT 
GAGAATCAGG ACGATAAGGA TACGGAACTG GAACTTGTGG CCAGCAACGT CTATCCGGTG 
CTCCGTCGCT ACCTCACCCA GATCAAGCTG GACAAGAGCG ATGTGCGTGC ACGGGAGCGT 
CTGGAGCGCA TCTGTCGTTA CATCATCAAG GCAATGGAGG CGGAGAGCAC CAAGCTATCC 
TATGCCGCCC TCTGTCTCTT CAAGGAACGC AGCCTGCCCT GGATCGCGCA CATTAAGATA 
CTGCTCACGA ACTGCCTAAC ATTGCTGCCG GAGCTGAAGC CGGAGAACCA TGCCGACAGC 
TTGTCCCTTG CCCTCTTCCT GCACACACTG GTGGTCTTCA CGGCGCCCAA ATCCTGGGCG 
ATACTCAGGA ATGCCCAGTT CGAGAAACTG CAGCCGGCCA TGCAAAAAAT CTGCTGTAAT 
ATTCAAGGTC ATCTGGTGCA GCACGATTTC TACAAAATCA TGCGGCTCGT TTTGCTGCGC 
GGCACTGTGC GCGAGGAGTT GTCTGTGAAG CCCGTCACCC TGGTGGCCAT CATTACGCTA 
TGCCTTCGTC CCCTGATCGA TGGCAACTTT AGTCGAAATC TACTGGGCAA ATTTCTGTCG 
GAGATTCTGT CTGTGCCCGC TTTAATCTAC CATCTGCACC AGAGTGTGCC GCAGTGCCTG 
GAACAGTTCT CATCTTTGGG GCTGCTTAAA AAGGCGCTTA GCATTTCGGG GGACGTGCAG 
TGGTTCGATG AATTCGGCAC GAGCATGCCG GGCACAAAAT CCCTGGCTTT TCTGGGAAAC 
ATTGTGAATT TGTTCAACAT TGATGGCCAA GGGGAGTCCA AGGAGCTGGC CTATCCACTA 
CTGACTGAAA CCACCACATC GCTGTTGGAA CTAATACCCA ACACGGTGAC CACCAAGGGA 
GTGTTCACTC AGTGGCACGA GCTGTTAGGA TGGCATACTC CGGGTCCAGA GCCTGCACAG 
AATCAGAATG TGGCCCTGAT TAAGAAGCAG TTTCACATGT TGTGGGATCA TCGCTGCATA 
AAGCTGTTGC TGGGAGATCT CTTGAAGCAA ATCAATATCA ACTACGAGCG AATCGAGTTC 
CAATCTCCCC AACAACCATC CACATCGAAT CTGCTGCGTC GGGCCCTGGA GCGTAGCTCC 
ACTAGAGGTG TTAACCTAAT GGGAGTAGCG AGCAGCAAGC AGGCTAAGCA GCAGTGGCGC 
AAGCTGGACA ATGCGGAGGT GGTGCAGGTG TCCCGAATCT GCGGCATGTA CTATGCCGCC 
CTCAACACCC TTTCCCAAAT GAAGCTGGAC ATCCTGACTG GCATTTGCTA CAACGACAAT 
GTGCTGTATG ATATTTGGTT GCTAATCACG TCCCTTGGCC CCAATTGCGG CATGAAGGAG 
TATCTGGAGC TGCTCAAATC CGAGTCAAAC ATGCAGAAGC CACAGACCGC CATGCTGATG 
CTCTTTTGCG ACTGCATGAC GCACTACGTA ACGATTCTGG ATGAATACGA AATGTACACG 
GAGCAGAATC CCTTTAAACT TAACGACTAT GTGATGCTGA CCTACTTCCT AAATAATATT 
CTCTACAAAC TTATTAACGA TAATCTTCTG GGTGCCAAGA ACATTGTCCA GAATCCGGTT 
TTCCTCTCGC TGCACACGCT GATGCTTTGC TTGTACCGCC GTGATTGCCG TCGCCCCTTC 
ACGCCGCCCA ACCACTGGCT GATACCGGAG GTCAAACCCT CCACGTTCAT CAACGACCTG 
GAAAAGGCCA AGAGGAACGC GATGCTGCTG CTCGCCAAGA TGCCTCACAT TATACCGCAC 
GAGGATCGGG TCAAGCTATT CCGGAAGTTC GTGCAGAATG AGAAGGCGGT GATGGGTCTG 
ACGGAGAGTG CCTGCGCTTC TCCTCGCTCC GCACTGATTG TCATCCATCG CGATCGTATT 
GTGGAGGATG GATACCGGCA ACTGGCCGCC CAGCCCACGC AAGCCCTAAA AGGTGTTATC 
CGCGTACGTT TCATCAATCA ACAGGGACTG CACGAGGCAG GAATCGATCA GGATGGAGTG 
TTCAAGGAAT TCCTTGAGGA GACCATCAAA AAGGTGTTCG ACCCGTCGCT GAATCTCTTC 
AAAACCACGT CGGATCAGCG GCTATATCCC TCGCCCATTT CCTACGTCCA GGACAATCAT 
TTGCAGCTCT TCGAGTTCGT GGGCCGCATG CTGGGCAAGG CGGTGTACGA AGGCATCGTG 
GTGGACGTCC CGTTTGCCTC CTTCTTCCTC TCCCAGCTTC TCGGCCAGAC GCAACAGGCG 
CTGTACTCCT GCATGGACGA GCTGCCGTCG CTGGACAACG AGTTGTATCG CAGTCTAACC 
TTCATCAAAC ACTATAAGCA AGATGTGTCC GATCTGAACC TTACCTTTTC CGTGGACCAG 
GATGTGATGG GCAAAATTGT CACTCTGGCA TTGCATCCGG GTGGCAAGTC GCGTGTGGTT 
AATGACCACA ACAAGCTGGT CTACATACAC TACATGGCAT TTTTCCACAT GAACACCCAG 
ATCCGCGAGC AGACGATAGC CTTCAATCGT GGCTTCCGCA GCATCGTCAA TCCAGAATGG 
TTGTCCCTTT TCTCGCCTCC GGAATTACAG CGCCTGATTT CTGGGGACAC CAGTCCATTG 
GACTTGAAGG ATCTGCGAAA GCATACACAC TATTACGGTG GCTTCCACGA CTCCCATCGC 
GTGGTTGGCT GGCTCTGGGA TATCCTGGCC AAGGATTTCA CGGAGGAGGA GCGTAAGCTG 
TTCCTAAAGT TTGTTACTAG CTGCTCAAAG CCTCCGCTTT TGGGTTTCGC CCACCTGGAG 
CCACCCTTCT CGATTCGTTG CGTGGAGGTG AGTGATGACG AGGATACGGG CGACACAATC 
GGAAGTGTGA TTCGCGGTTT CTTTACCATA CGTAAAAAGG ATCCACTCAA CCGCCTACCC 
ACCTCGTCGA CCTGCTTCAA CCTGCTGAAG CTGCCCAACT ACCAAAAGAA GTCCACGCTG 
AGGGACAAAC TTCGCTATGC TGTGAGCAGC AACACCGGAT TCGAGCTGTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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LOC6544975 cDNA ORF clone, Drosophila erecta

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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