LOC6540960 cDNA ORF clone, Drosophila erecta

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC6540960
Entrez Gene ID	6540960
Full Name	GG23202 gene product from transcript GG23202-RA
Synonyms	GG23202,dere_GLEANR_8046
Gene Type	protein-coding
Organism	Drosophila erecta

mRNA and Protein(s)

mRNA	Protein	Name
XM_001970731.2	XP_001970767.1	uncharacterized protein
XM_001970731.3	XP_001970767.1	phospholipase D2 isoform X1
XM_026979099.1	XP_026834900.1	phospholipase D2 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC6540960 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC6540960 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODi00363	XM_001970731.3 Latest version!	Drosophila erecta phospholipase D2 (LOC6540960), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODi218997	XM_026979099.1 Latest version!	Drosophila erecta phospholipase D2 (LOC6540960), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODi00363	XM_001970731.2	Drosophila erecta uncharacterized protein (Dere\GG23202), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC6540960 ( XM_001970731.2 ) cDNA ORF clone, Drosophila erecta -> XP_001970767.1 Drosophila erecta uncharacterized protein (Dere\GG23202), mRNA.

Price & Availability↑

CloneID	ODi00363
Clone ID Related Accession (Same CDS sequence)	XM_001970731.2 , XM_001970731.3
Accession Version	XM_001970731.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956548). On Dec 21, 2015 this sequence version replaced XM_001970731.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGCCA AGAGCGTGGA TACCACAACC AGCAACAACC ATGAGGCCCA GGAACTGCAG 
CTGCCCCGGC AGGCGTCACC GAGCGCCAAC TTGCCACATT TGCAGGTCCG ACGTCATCGC 
CGCAGCATTT CACAGCTGAT GGCCAGCATG GTTGAACACC CCAATGACGA TGTGTACCGG 
CCGGCGCACT ACGGCGGCTA CGTCAACAGA GGCTACGATG ATATAGACAG TTCCTACTAC 
TTTGCCCAGT TCGAGGCGAT GGCAGATGCC AGCACCGTTG GAGGCGCCTT GCCGCCCTAC 
GCACTCAGCA ACTCGGACGA CGAACGCGGC AGCGGGGAAG AGGACGCGTC GGGGGAGAAC 
TCCAACAATG ATGAGGGGGA GGTGTTCCCG GACTGCACGG ACGAAGCGGC AGAGGAGCAT 
CAAAACCGCT GCTTGCCAGA ATTTCAGTTC TCCCTGGTGG ATTCTGAGTA CGATGAGACC 
CTCGCCTTTC CTGACTCGGT GACCATCCTA TCCAACGTGG GCGACAAGCC GGTGCTGGTG 
GAGCGCAAGG AGACGGACGA CGATGAGGAG GAGTTTGACG ACGAGGAAAA CAACAGTGTA 
GTCCTGCGAC ACGAAATACC ATTCACCAGC ATATACGGGC CGAGCGTCAA GTTCAACTCG 
TTTCAGCGCA AGGTCTTCAT TCCCGGACGT GAGATTCATG TCCGGATCGT CGATACGGAA 
CGCAGCGTCA CCACCCATCT GCTAAATCCC AACCTATACA CAATCGAGCT GACCCATGGT 
CCCTTCAAAT GGACGATCAA GCGGCGCTAC AAGCACTTTA ACTCGCTGCA CCAGCAGCTC 
AGCTTTTTCC GCACCTCGCT CAACATTCCC TTTCCCAGTC GCAGTCACAA GGAGAAGCGT 
ACCACGCTGA AGGCCACTGC CAGAGAGATG GCTGACGAGT CCACTTTGAA GGACCTTCCT 
TCCCACACCA AGGTCAAGCA GACTAGCACA CCGCTGAGGA CTGACGGCAG GAGCAGCAAA 
ATCGCCAGCA GTAACGGCAA CAATGCCATG GCTATCCTAA GTCCCAATCA CAGCTCCATT 
CTGGCGGGTT TTACGCCGCG ACGCATTCAA AAGAAGCGCA AAAAAAAGAA GAAACGGAAG 
CTACCGCGGT TCCCCAATCG TCCTGAGAGT CTGGTCACCG TGGAGAACCT GAGCGTCAGG 
ATCAAGCAGC TGGAGGACTA CTTGTACAAC CTGCTGAACA TAAGTTTGTA CCGATCCCAC 
CACGAAACGC TAAACTTCGT GGAGGCGTCC AATGTGTCCT TCGTTCCGGG AATGGGCATT 
AAGGGCAAGG AAGGATTGAT TTTAAAGCGA ACTGGATCAA CGAGACCGGG GCAAGCGGGA 
TGCAATTTCT TCGGCTGCTT TCAAAAGAAC TGCTGCGTGC GCTGCAACTA CTTTTGCTCC 
GACGTAGTGT GCGGCACGTG GCGGAACCGA TGGTTCTTCG TAAAAGAGAC CTGCTTCGGC 
TACATCCGCC CAACAGACGG CAGCATCCGG GCAGTGATCC TTTTCGATCA GGGCTTCGAC 
GTGTCCACGG GTATCTACCA GACGGGAATG CGCAAGGGCT TGCAGGTGCT GACGAACAAC 
CGTCACATTG TGCTCAAGTG CTGGACACGG CGCAAGTGTA AGGAGTGGAT GCAGTACCTC 
AAGAACATGG CCAACTCGTA TGCGCGCGAC TTCACCCTGC CCAATCCGCA CATGGCCTTC 
GCACCGATGC GCGCCAACAG CCATGCCACG TGGTATGTAG ACGGCGCCCA GTACATGTCA 
GCCGTGGCCG ACGGCTTGGA GGCAGCCCTC GAGGAGATCT ACATTGCCGA CTGGTGGCTC 
AGCCCCGAGA TTTACATGAA GCGACCCGCC CTCGACGGAG ACTACTGGCG TCTGGACAAG 
ATCTTGTTGC GAAAAGCCGA ACAGGGCGTG CGTATCTTCG TGCTGCTATA CAAGGAGGTC 
GAAATGGCTC TTGGCATCAA CAGCTACTAC AGCAAGTCCA CGCTGGCCAA GCACGAAAAC 
ATCAAAGTCA TGCGTCATCC GGACCACGCT AGAGGTGGTA TTTTGCTGTG GGCCCATCAC 
GAAAAGATCG TCGTCATCGA TCAGACCTAT GCCTTCATGG GAGGTATTGA TTTATGCTAT 
GGGCGTTGGG ACGACCACCA TCATCGGCTC ACGGATCTGG GAAGCATATC CACGGCATCT 
TTTTCTGGCA GCGCGCGTCG AACGCCGAGC CTATACTTCA CCAAAGACGA CACGGACTCA 
GCTTTCGGAT CACGAAAGTC GTCGCGGAAT GCCCACTACG ATATGTCCGC CAAGGAGAGG 
CCACCGTCTT CACCCCCGCA GGAGCCCAGT ACTAGCATAG AGTTGAAAAC TCTAAAGCCA 
GGTGATCGCC TGCTTATACC CTCTACGCTC GTTTCGAGTC CGGGCGACAC TCCCGCTGAA 
TCGGGAATCG CTTTAGAGGG AATGAAACTC AACACCCCTG AAATGGAGCG CAAGAACGTA 
CTCGATCGCC TGAAGAACAA CGCGATGAAG GGCGCCCGCA TGGGCAAGGA CTTTATGCAC 
CGACTAACAG CCACGGAGGC GGAGGAAAAA TCAGCCGAGG TGTACACTAT CGAGTCCGAG 
GATGTCACGG ACCACGAAGT CAACCCTAAC ATGGCTTCAG GTGGGGGGGA GGTGGCAATT 
ACCACTAGCA GCACACAAAT ACTCAGTGAG TTCTGCGGCC AGGCCAAGTA CTGGTTCGGC 
AAGGATTACT CCAACTTTAT ACTTAAGGAC TGGATGAACC TAAACTCGCC GTTCGTGGAT 
ATCATAGATC GAACAACAAC ACCGCGGATG CCCTGGCACG ACGTAGGTCT GTGTGTGGTG 
GGTACTTCCG CTAGGGATGT GGCCCGCCAC TTCATCCAGC GCTGGAACGC CATGAAGTTG 
GAGAAACTGC GCGAGAACAC AAGATTCCCC TATCTGATGC CAAAGAGCTA TCACCAAGTG 
AGGCTGAATC CGACCCTGCA GCAAAACCGT CAGCAGCGGG TCACGTGCCA GCTACTTCGG 
AGCGTCTCCG CCTGGAGCTG CGGCTTTATA GAGGCGGATC TGGTGGAGCA GAGCATCCAC 
GATGCCTACA TCCAGACGAT CACCAAGGCG CAGCACTACG TGTACATCGA AAACCAATTT 
TTCATCACCA TGCAGTTGGG CATGGGTGTG CCAGGCGCCC ATAACAATGT GCGGAATCAA 
ATCGGGGAGA CACTCTTTAA GCGGATTGTA CGGGCGCACA AGGAACGTAA GCCTTTCCGA 
GTTTATGTGA TTATGCCGCT CCTTCCGGGC TTCGAGGGCG ACGTGGGTGG CAGTACTGGG 
ATAGCAGTCA GAGCCATTAC GCACTGGAAC TATGCATCCA TTTCCAGGGG ACGCTCCGCA 
ATTTTGAACC GACTGCAGGA GATGGGTATT GCCAATCCGC AAAACTACAT CTCATTCCAC 
AGCCTGCGCA ACCATTCCTT CTTGAACAAC ACACCCATAA CGGAGCTGAT ATATGTCCAC 
TCAAAGCTCC TGATAGCCGA CGATCGTGTG GTGATCTGTG GTTCGGCGAA CATTAACGAT 
CGCTCAATGA TCGGGAAGCG GGACTCCGAG ATAGCGGCTA TCATTATGGA TGAGGAGTTT 
GAGGACGGAC GCATGAATGG CAAGAAGTAT CCGAGCGGAG TGTTTGCCGG TCGCCTTCGA 
AAATATCTTT TTAAAGAACA CTTGGGCCTC CTGGAAAGCG AAGGTTCCAG TCGGTCCGAC 
CTGGACATTA GTGATCCTGT TTGCGACAAG TTTTGGCACG GCACCTGGCG TAGGATTTCA 
ACCCAGAACA CTGAGATTTA CGACGAGGTC TTTAAGTGCA TCCCCACAGA CTTTGTAAAG 
ACCTTTGCCA GCCTTCGCAA ATACCAGGAG GAGCCGCCGC TTGCCAAGAC CGACCCAGAG 
CTAGCTGCCA ACAGGGCCAA CGACATTCAG GGTTACCTGG TCAACCTGCC ATTGGACTTC 
CTGAACAAGG AGGTCCTTAC GCCGCCTGGA ACTAGTAAGG AAGGGCTAAT TCCTACCTCT 
GTATGGACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001970767.1
CDS	148..4239
Translation	MMAKSVDTTTSNNHEAQELQLPRQASPSANLPHLQVRRHRRSISQLMASMVEHPNDDVYRPAHYGGYVNRGYDDIDSSYYFAQFEAMADASTVGGALPPYALSNSDDERGSGEEDASGENSNNDEGEVFPDCTDEAAEEHQNRCLPEFQFSLVDSEYDETLAFPDSVTILSNVGDKPVLVERKETDDDEEEFDDEENNSVVLRHEIPFTSIYGPSVKFNSFQRKVFIPGREIHVRIVDTERSVTTHLLNPNLYTIELTHGPFKWTIKRRYKHFNSLHQQLSFFRTSLNIPFPSRSHKEKRTTLKATAREMADESTLKDLPSHTKVKQTSTPLRTDGRSSKIASSNGNNAMAILSPNHSSILAGFTPRRIQKKRKKKKKRKLPRFPNRPESLVTVENLSVRIKQLEDYLYNLLNISLYRSHHETLNFVEASNVSFVPGMGIKGKEGLILKRTGSTRPGQAGCNFFGCFQKNCCVRCNYFCSDVVCGTWRNRWFFVKETCFGYIRPTDGSIRAVILFDQGFDVSTGIYQTGMRKGLQVLTNNRHIVLKCWTRRKCKEWMQYLKNMANSYARDFTLPNPHMAFAPMRANSHATWYVDGAQYMSAVADGLEAALEEIYIADWWLSPEIYMKRPALDGDYWRLDKILLRKAEQGVRIFVLLYKEVEMALGINSYYSKSTLAKHENIKVMRHPDHARGGILLWAHHEKIVVIDQTYAFMGGIDLCYGRWDDHHHRLTDLGSISTASFSGSARRTPSLYFTKDDTDSAFGSRKSSRNAHYDMSAKERPPSSPPQEPSTSIELKTLKPGDRLLIPSTLVSSPGDTPAESGIALEGMKLNTPEMERKNVLDRLKNNAMKGARMGKDFMHRLTATEAEEKSAEVYTIESEDVTDHEVNPNMASGGGEVAITTSSTQILSEFCGQAKYWFGKDYSNFILKDWMNLNSPFVDIIDRTTTPRMPWHDVGLCVVGTSARDVARHFIQRWNAMKLEKLRENTRFPYLMPKSYHQVRLNPTLQQNRQQRVTCQLLRSVSAWSCGFIEADLVEQSIHDAYIQTITKAQHYVYIENQFFITMQLGMGVPGAHNNVRNQIGETLFKRIVRAHKERKPFRVYVIMPLLPGFEGDVGGSTGIAVRAITHWNYASISRGRSAILNRLQEMGIANPQNYISFHSLRNHSFLNNTPITELIYVHSKLLIADDRVVICGSANINDRSMIGKRDSEIAAIIMDEEFEDGRMNGKKYPSGVFAGRLRKYLFKEHLGLLESEGSSRSDLDISDPVCDKFWHGTWRRISTQNTEIYDEVFKCIPTDFVKTFASLRKYQEEPPLAKTDPELAANRANDIQGYLVNLPLDFLNKEVLTPPGTSKEGLIPTSVWT

Target ORF information:

RefSeq Version	XM_001970731.2
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein (Dere\GG23202), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001970731.2

ORF Insert Sequence:

ATGATGGCCA AGAGCGTGGA TACCACAACC AGCAACAACC ATGAGGCCCA GGAACTGCAG 
CTGCCCCGGC AGGCGTCACC GAGCGCCAAC TTGCCACATT TGCAGGTCCG ACGTCATCGC 
CGCAGCATTT CACAGCTGAT GGCCAGCATG GTTGAACACC CCAATGACGA TGTGTACCGG 
CCGGCGCACT ACGGCGGCTA CGTCAACAGA GGCTACGATG ATATAGACAG TTCCTACTAC 
TTTGCCCAGT TCGAGGCGAT GGCAGATGCC AGCACCGTTG GAGGCGCCTT GCCGCCCTAC 
GCACTCAGCA ACTCGGACGA CGAACGCGGC AGCGGGGAAG AGGACGCGTC GGGGGAGAAC 
TCCAACAATG ATGAGGGGGA GGTGTTCCCG GACTGCACGG ACGAAGCGGC AGAGGAGCAT 
CAAAACCGCT GCTTGCCAGA ATTTCAGTTC TCCCTGGTGG ATTCTGAGTA CGATGAGACC 
CTCGCCTTTC CTGACTCGGT GACCATCCTA TCCAACGTGG GCGACAAGCC GGTGCTGGTG 
GAGCGCAAGG AGACGGACGA CGATGAGGAG GAGTTTGACG ACGAGGAAAA CAACAGTGTA 
GTCCTGCGAC ACGAAATACC ATTCACCAGC ATATACGGGC CGAGCGTCAA GTTCAACTCG 
TTTCAGCGCA AGGTCTTCAT TCCCGGACGT GAGATTCATG TCCGGATCGT CGATACGGAA 
CGCAGCGTCA CCACCCATCT GCTAAATCCC AACCTATACA CAATCGAGCT GACCCATGGT 
CCCTTCAAAT GGACGATCAA GCGGCGCTAC AAGCACTTTA ACTCGCTGCA CCAGCAGCTC 
AGCTTTTTCC GCACCTCGCT CAACATTCCC TTTCCCAGTC GCAGTCACAA GGAGAAGCGT 
ACCACGCTGA AGGCCACTGC CAGAGAGATG GCTGACGAGT CCACTTTGAA GGACCTTCCT 
TCCCACACCA AGGTCAAGCA GACTAGCACA CCGCTGAGGA CTGACGGCAG GAGCAGCAAA 
ATCGCCAGCA GTAACGGCAA CAATGCCATG GCTATCCTAA GTCCCAATCA CAGCTCCATT 
CTGGCGGGTT TTACGCCGCG ACGCATTCAA AAGAAGCGCA AAAAAAAGAA GAAACGGAAG 
CTACCGCGGT TCCCCAATCG TCCTGAGAGT CTGGTCACCG TGGAGAACCT GAGCGTCAGG 
ATCAAGCAGC TGGAGGACTA CTTGTACAAC CTGCTGAACA TAAGTTTGTA CCGATCCCAC 
CACGAAACGC TAAACTTCGT GGAGGCGTCC AATGTGTCCT TCGTTCCGGG AATGGGCATT 
AAGGGCAAGG AAGGATTGAT TTTAAAGCGA ACTGGATCAA CGAGACCGGG GCAAGCGGGA 
TGCAATTTCT TCGGCTGCTT TCAAAAGAAC TGCTGCGTGC GCTGCAACTA CTTTTGCTCC 
GACGTAGTGT GCGGCACGTG GCGGAACCGA TGGTTCTTCG TAAAAGAGAC CTGCTTCGGC 
TACATCCGCC CAACAGACGG CAGCATCCGG GCAGTGATCC TTTTCGATCA GGGCTTCGAC 
GTGTCCACGG GTATCTACCA GACGGGAATG CGCAAGGGCT TGCAGGTGCT GACGAACAAC 
CGTCACATTG TGCTCAAGTG CTGGACACGG CGCAAGTGTA AGGAGTGGAT GCAGTACCTC 
AAGAACATGG CCAACTCGTA TGCGCGCGAC TTCACCCTGC CCAATCCGCA CATGGCCTTC 
GCACCGATGC GCGCCAACAG CCATGCCACG TGGTATGTAG ACGGCGCCCA GTACATGTCA 
GCCGTGGCCG ACGGCTTGGA GGCAGCCCTC GAGGAGATCT ACATTGCCGA CTGGTGGCTC 
AGCCCCGAGA TTTACATGAA GCGACCCGCC CTCGACGGAG ACTACTGGCG TCTGGACAAG 
ATCTTGTTGC GAAAAGCCGA ACAGGGCGTG CGTATCTTCG TGCTGCTATA CAAGGAGGTC 
GAAATGGCTC TTGGCATCAA CAGCTACTAC AGCAAGTCCA CGCTGGCCAA GCACGAAAAC 
ATCAAAGTCA TGCGTCATCC GGACCACGCT AGAGGTGGTA TTTTGCTGTG GGCCCATCAC 
GAAAAGATCG TCGTCATCGA TCAGACCTAT GCCTTCATGG GAGGTATTGA TTTATGCTAT 
GGGCGTTGGG ACGACCACCA TCATCGGCTC ACGGATCTGG GAAGCATATC CACGGCATCT 
TTTTCTGGCA GCGCGCGTCG AACGCCGAGC CTATACTTCA CCAAAGACGA CACGGACTCA 
GCTTTCGGAT CACGAAAGTC GTCGCGGAAT GCCCACTACG ATATGTCCGC CAAGGAGAGG 
CCACCGTCTT CACCCCCGCA GGAGCCCAGT ACTAGCATAG AGTTGAAAAC TCTAAAGCCA 
GGTGATCGCC TGCTTATACC CTCTACGCTC GTTTCGAGTC CGGGCGACAC TCCCGCTGAA 
TCGGGAATCG CTTTAGAGGG AATGAAACTC AACACCCCTG AAATGGAGCG CAAGAACGTA 
CTCGATCGCC TGAAGAACAA CGCGATGAAG GGCGCCCGCA TGGGCAAGGA CTTTATGCAC 
CGACTAACAG CCACGGAGGC GGAGGAAAAA TCAGCCGAGG TGTACACTAT CGAGTCCGAG 
GATGTCACGG ACCACGAAGT CAACCCTAAC ATGGCTTCAG GTGGGGGGGA GGTGGCAATT 
ACCACTAGCA GCACACAAAT ACTCAGTGAG TTCTGCGGCC AGGCCAAGTA CTGGTTCGGC 
AAGGATTACT CCAACTTTAT ACTTAAGGAC TGGATGAACC TAAACTCGCC GTTCGTGGAT 
ATCATAGATC GAACAACAAC ACCGCGGATG CCCTGGCACG ACGTAGGTCT GTGTGTGGTG 
GGTACTTCCG CTAGGGATGT GGCCCGCCAC TTCATCCAGC GCTGGAACGC CATGAAGTTG 
GAGAAACTGC GCGAGAACAC AAGATTCCCC TATCTGATGC CAAAGAGCTA TCACCAAGTG 
AGGCTGAATC CGACCCTGCA GCAAAACCGT CAGCAGCGGG TCACGTGCCA GCTACTTCGG 
AGCGTCTCCG CCTGGAGCTG CGGCTTTATA GAGGCGGATC TGGTGGAGCA GAGCATCCAC 
GATGCCTACA TCCAGACGAT CACCAAGGCG CAGCACTACG TGTACATCGA AAACCAATTT 
TTCATCACCA TGCAGTTGGG CATGGGTGTG CCAGGCGCCC ATAACAATGT GCGGAATCAA 
ATCGGGGAGA CACTCTTTAA GCGGATTGTA CGGGCGCACA AGGAACGTAA GCCTTTCCGA 
GTTTATGTGA TTATGCCGCT CCTTCCGGGC TTCGAGGGCG ACGTGGGTGG CAGTACTGGG 
ATAGCAGTCA GAGCCATTAC GCACTGGAAC TATGCATCCA TTTCCAGGGG ACGCTCCGCA 
ATTTTGAACC GACTGCAGGA GATGGGTATT GCCAATCCGC AAAACTACAT CTCATTCCAC 
AGCCTGCGCA ACCATTCCTT CTTGAACAAC ACACCCATAA CGGAGCTGAT ATATGTCCAC 
TCAAAGCTCC TGATAGCCGA CGATCGTGTG GTGATCTGTG GTTCGGCGAA CATTAACGAT 
CGCTCAATGA TCGGGAAGCG GGACTCCGAG ATAGCGGCTA TCATTATGGA TGAGGAGTTT 
GAGGACGGAC GCATGAATGG CAAGAAGTAT CCGAGCGGAG TGTTTGCCGG TCGCCTTCGA 
AAATATCTTT TTAAAGAACA CTTGGGCCTC CTGGAAAGCG AAGGTTCCAG TCGGTCCGAC 
CTGGACATTA GTGATCCTGT TTGCGACAAG TTTTGGCACG GCACCTGGCG TAGGATTTCA 
ACCCAGAACA CTGAGATTTA CGACGAGGTC TTTAAGTGCA TCCCCACAGA CTTTGTAAAG 
ACCTTTGCCA GCCTTCGCAA ATACCAGGAG GAGCCGCCGC TTGCCAAGAC CGACCCAGAG 
CTAGCTGCCA ACAGGGCCAA CGACATTCAG GGTTACCTGG TCAACCTGCC ATTGGACTTC 
CTGAACAAGG AGGTCCTTAC GCCGCCTGGA ACTAGTAAGG AAGGGCTAAT TCCTACCTCT 
GTATGGACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6540960 ( XM_001970731.3 ) cDNA ORF clone, Drosophila erecta -> XP_001970767.1 Drosophila erecta phospholipase D2 (LOC6540960), transcript variant X1, mRNA.

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CloneID	ODi00363
Clone ID Related Accession (Same CDS sequence)	XM_001970731.2 , XM_001970731.3
Accession Version	XM_001970731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	phospholipase D2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825200.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_001970731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCA AGAGCGTGGA TACCACAACC AGCAACAACC ATGAGGCCCA GGAACTGCAG 
CTGCCCCGGC AGGCGTCACC GAGCGCCAAC TTGCCACATT TGCAGGTCCG ACGTCATCGC 
CGCAGCATTT CACAGCTGAT GGCCAGCATG GTTGAACACC CCAATGACGA TGTGTACCGG 
CCGGCGCACT ACGGCGGCTA CGTCAACAGA GGCTACGATG ATATAGACAG TTCCTACTAC 
TTTGCCCAGT TCGAGGCGAT GGCAGATGCC AGCACCGTTG GAGGCGCCTT GCCGCCCTAC 
GCACTCAGCA ACTCGGACGA CGAACGCGGC AGCGGGGAAG AGGACGCGTC GGGGGAGAAC 
TCCAACAATG ATGAGGGGGA GGTGTTCCCG GACTGCACGG ACGAAGCGGC AGAGGAGCAT 
CAAAACCGCT GCTTGCCAGA ATTTCAGTTC TCCCTGGTGG ATTCTGAGTA CGATGAGACC 
CTCGCCTTTC CTGACTCGGT GACCATCCTA TCCAACGTGG GCGACAAGCC GGTGCTGGTG 
GAGCGCAAGG AGACGGACGA CGATGAGGAG GAGTTTGACG ACGAGGAAAA CAACAGTGTA 
GTCCTGCGAC ACGAAATACC ATTCACCAGC ATATACGGGC CGAGCGTCAA GTTCAACTCG 
TTTCAGCGCA AGGTCTTCAT TCCCGGACGT GAGATTCATG TCCGGATCGT CGATACGGAA 
CGCAGCGTCA CCACCCATCT GCTAAATCCC AACCTATACA CAATCGAGCT GACCCATGGT 
CCCTTCAAAT GGACGATCAA GCGGCGCTAC AAGCACTTTA ACTCGCTGCA CCAGCAGCTC 
AGCTTTTTCC GCACCTCGCT CAACATTCCC TTTCCCAGTC GCAGTCACAA GGAGAAGCGT 
ACCACGCTGA AGGCCACTGC CAGAGAGATG GCTGACGAGT CCACTTTGAA GGACCTTCCT 
TCCCACACCA AGGTCAAGCA GACTAGCACA CCGCTGAGGA CTGACGGCAG GAGCAGCAAA 
ATCGCCAGCA GTAACGGCAA CAATGCCATG GCTATCCTAA GTCCCAATCA CAGCTCCATT 
CTGGCGGGTT TTACGCCGCG ACGCATTCAA AAGAAGCGCA AAAAAAAGAA GAAACGGAAG 
CTACCGCGGT TCCCCAATCG TCCTGAGAGT CTGGTCACCG TGGAGAACCT GAGCGTCAGG 
ATCAAGCAGC TGGAGGACTA CTTGTACAAC CTGCTGAACA TAAGTTTGTA CCGATCCCAC 
CACGAAACGC TAAACTTCGT GGAGGCGTCC AATGTGTCCT TCGTTCCGGG AATGGGCATT 
AAGGGCAAGG AAGGATTGAT TTTAAAGCGA ACTGGATCAA CGAGACCGGG GCAAGCGGGA 
TGCAATTTCT TCGGCTGCTT TCAAAAGAAC TGCTGCGTGC GCTGCAACTA CTTTTGCTCC 
GACGTAGTGT GCGGCACGTG GCGGAACCGA TGGTTCTTCG TAAAAGAGAC CTGCTTCGGC 
TACATCCGCC CAACAGACGG CAGCATCCGG GCAGTGATCC TTTTCGATCA GGGCTTCGAC 
GTGTCCACGG GTATCTACCA GACGGGAATG CGCAAGGGCT TGCAGGTGCT GACGAACAAC 
CGTCACATTG TGCTCAAGTG CTGGACACGG CGCAAGTGTA AGGAGTGGAT GCAGTACCTC 
AAGAACATGG CCAACTCGTA TGCGCGCGAC TTCACCCTGC CCAATCCGCA CATGGCCTTC 
GCACCGATGC GCGCCAACAG CCATGCCACG TGGTATGTAG ACGGCGCCCA GTACATGTCA 
GCCGTGGCCG ACGGCTTGGA GGCAGCCCTC GAGGAGATCT ACATTGCCGA CTGGTGGCTC 
AGCCCCGAGA TTTACATGAA GCGACCCGCC CTCGACGGAG ACTACTGGCG TCTGGACAAG 
ATCTTGTTGC GAAAAGCCGA ACAGGGCGTG CGTATCTTCG TGCTGCTATA CAAGGAGGTC 
GAAATGGCTC TTGGCATCAA CAGCTACTAC AGCAAGTCCA CGCTGGCCAA GCACGAAAAC 
ATCAAAGTCA TGCGTCATCC GGACCACGCT AGAGGTGGTA TTTTGCTGTG GGCCCATCAC 
GAAAAGATCG TCGTCATCGA TCAGACCTAT GCCTTCATGG GAGGTATTGA TTTATGCTAT 
GGGCGTTGGG ACGACCACCA TCATCGGCTC ACGGATCTGG GAAGCATATC CACGGCATCT 
TTTTCTGGCA GCGCGCGTCG AACGCCGAGC CTATACTTCA CCAAAGACGA CACGGACTCA 
GCTTTCGGAT CACGAAAGTC GTCGCGGAAT GCCCACTACG ATATGTCCGC CAAGGAGAGG 
CCACCGTCTT CACCCCCGCA GGAGCCCAGT ACTAGCATAG AGTTGAAAAC TCTAAAGCCA 
GGTGATCGCC TGCTTATACC CTCTACGCTC GTTTCGAGTC CGGGCGACAC TCCCGCTGAA 
TCGGGAATCG CTTTAGAGGG AATGAAACTC AACACCCCTG AAATGGAGCG CAAGAACGTA 
CTCGATCGCC TGAAGAACAA CGCGATGAAG GGCGCCCGCA TGGGCAAGGA CTTTATGCAC 
CGACTAACAG CCACGGAGGC GGAGGAAAAA TCAGCCGAGG TGTACACTAT CGAGTCCGAG 
GATGTCACGG ACCACGAAGT CAACCCTAAC ATGGCTTCAG GTGGGGGGGA GGTGGCAATT 
ACCACTAGCA GCACACAAAT ACTCAGTGAG TTCTGCGGCC AGGCCAAGTA CTGGTTCGGC 
AAGGATTACT CCAACTTTAT ACTTAAGGAC TGGATGAACC TAAACTCGCC GTTCGTGGAT 
ATCATAGATC GAACAACAAC ACCGCGGATG CCCTGGCACG ACGTAGGTCT GTGTGTGGTG 
GGTACTTCCG CTAGGGATGT GGCCCGCCAC TTCATCCAGC GCTGGAACGC CATGAAGTTG 
GAGAAACTGC GCGAGAACAC AAGATTCCCC TATCTGATGC CAAAGAGCTA TCACCAAGTG 
AGGCTGAATC CGACCCTGCA GCAAAACCGT CAGCAGCGGG TCACGTGCCA GCTACTTCGG 
AGCGTCTCCG CCTGGAGCTG CGGCTTTATA GAGGCGGATC TGGTGGAGCA GAGCATCCAC 
GATGCCTACA TCCAGACGAT CACCAAGGCG CAGCACTACG TGTACATCGA AAACCAATTT 
TTCATCACCA TGCAGTTGGG CATGGGTGTG CCAGGCGCCC ATAACAATGT GCGGAATCAA 
ATCGGGGAGA CACTCTTTAA GCGGATTGTA CGGGCGCACA AGGAACGTAA GCCTTTCCGA 
GTTTATGTGA TTATGCCGCT CCTTCCGGGC TTCGAGGGCG ACGTGGGTGG CAGTACTGGG 
ATAGCAGTCA GAGCCATTAC GCACTGGAAC TATGCATCCA TTTCCAGGGG ACGCTCCGCA 
ATTTTGAACC GACTGCAGGA GATGGGTATT GCCAATCCGC AAAACTACAT CTCATTCCAC 
AGCCTGCGCA ACCATTCCTT CTTGAACAAC ACACCCATAA CGGAGCTGAT ATATGTCCAC 
TCAAAGCTCC TGATAGCCGA CGATCGTGTG GTGATCTGTG GTTCGGCGAA CATTAACGAT 
CGCTCAATGA TCGGGAAGCG GGACTCCGAG ATAGCGGCTA TCATTATGGA TGAGGAGTTT 
GAGGACGGAC GCATGAATGG CAAGAAGTAT CCGAGCGGAG TGTTTGCCGG TCGCCTTCGA 
AAATATCTTT TTAAAGAACA CTTGGGCCTC CTGGAAAGCG AAGGTTCCAG TCGGTCCGAC 
CTGGACATTA GTGATCCTGT TTGCGACAAG TTTTGGCACG GCACCTGGCG TAGGATTTCA 
ACCCAGAACA CTGAGATTTA CGACGAGGTC TTTAAGTGCA TCCCCACAGA CTTTGTAAAG 
ACCTTTGCCA GCCTTCGCAA ATACCAGGAG GAGCCGCCGC TTGCCAAGAC CGACCCAGAG 
CTAGCTGCCA ACAGGGCCAA CGACATTCAG GGTTACCTGG TCAACCTGCC ATTGGACTTC 
CTGAACAAGG AGGTCCTTAC GCCGCCTGGA ACTAGTAAGG AAGGGCTAAT TCCTACCTCT 
GTATGGACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001970767.1
CDS	446..4537
Translation	MMAKSVDTTTSNNHEAQELQLPRQASPSANLPHLQVRRHRRSISQLMASMVEHPNDDVYRPAHYGGYVNRGYDDIDSSYYFAQFEAMADASTVGGALPPYALSNSDDERGSGEEDASGENSNNDEGEVFPDCTDEAAEEHQNRCLPEFQFSLVDSEYDETLAFPDSVTILSNVGDKPVLVERKETDDDEEEFDDEENNSVVLRHEIPFTSIYGPSVKFNSFQRKVFIPGREIHVRIVDTERSVTTHLLNPNLYTIELTHGPFKWTIKRRYKHFNSLHQQLSFFRTSLNIPFPSRSHKEKRTTLKATAREMADESTLKDLPSHTKVKQTSTPLRTDGRSSKIASSNGNNAMAILSPNHSSILAGFTPRRIQKKRKKKKKRKLPRFPNRPESLVTVENLSVRIKQLEDYLYNLLNISLYRSHHETLNFVEASNVSFVPGMGIKGKEGLILKRTGSTRPGQAGCNFFGCFQKNCCVRCNYFCSDVVCGTWRNRWFFVKETCFGYIRPTDGSIRAVILFDQGFDVSTGIYQTGMRKGLQVLTNNRHIVLKCWTRRKCKEWMQYLKNMANSYARDFTLPNPHMAFAPMRANSHATWYVDGAQYMSAVADGLEAALEEIYIADWWLSPEIYMKRPALDGDYWRLDKILLRKAEQGVRIFVLLYKEVEMALGINSYYSKSTLAKHENIKVMRHPDHARGGILLWAHHEKIVVIDQTYAFMGGIDLCYGRWDDHHHRLTDLGSISTASFSGSARRTPSLYFTKDDTDSAFGSRKSSRNAHYDMSAKERPPSSPPQEPSTSIELKTLKPGDRLLIPSTLVSSPGDTPAESGIALEGMKLNTPEMERKNVLDRLKNNAMKGARMGKDFMHRLTATEAEEKSAEVYTIESEDVTDHEVNPNMASGGGEVAITTSSTQILSEFCGQAKYWFGKDYSNFILKDWMNLNSPFVDIIDRTTTPRMPWHDVGLCVVGTSARDVARHFIQRWNAMKLEKLRENTRFPYLMPKSYHQVRLNPTLQQNRQQRVTCQLLRSVSAWSCGFIEADLVEQSIHDAYIQTITKAQHYVYIENQFFITMQLGMGVPGAHNNVRNQIGETLFKRIVRAHKERKPFRVYVIMPLLPGFEGDVGGSTGIAVRAITHWNYASISRGRSAILNRLQEMGIANPQNYISFHSLRNHSFLNNTPITELIYVHSKLLIADDRVVICGSANINDRSMIGKRDSEIAAIIMDEEFEDGRMNGKKYPSGVFAGRLRKYLFKEHLGLLESEGSSRSDLDISDPVCDKFWHGTWRRISTQNTEIYDEVFKCIPTDFVKTFASLRKYQEEPPLAKTDPELAANRANDIQGYLVNLPLDFLNKEVLTPPGTSKEGLIPTSVWT

Target ORF information:

RefSeq Version	XM_001970731.3
Organism	Drosophila erecta
Definition	Drosophila erecta phospholipase D2 (LOC6540960), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001970731.3

ORF Insert Sequence:

ATGATGGCCA AGAGCGTGGA TACCACAACC AGCAACAACC ATGAGGCCCA GGAACTGCAG 
CTGCCCCGGC AGGCGTCACC GAGCGCCAAC TTGCCACATT TGCAGGTCCG ACGTCATCGC 
CGCAGCATTT CACAGCTGAT GGCCAGCATG GTTGAACACC CCAATGACGA TGTGTACCGG 
CCGGCGCACT ACGGCGGCTA CGTCAACAGA GGCTACGATG ATATAGACAG TTCCTACTAC 
TTTGCCCAGT TCGAGGCGAT GGCAGATGCC AGCACCGTTG GAGGCGCCTT GCCGCCCTAC 
GCACTCAGCA ACTCGGACGA CGAACGCGGC AGCGGGGAAG AGGACGCGTC GGGGGAGAAC 
TCCAACAATG ATGAGGGGGA GGTGTTCCCG GACTGCACGG ACGAAGCGGC AGAGGAGCAT 
CAAAACCGCT GCTTGCCAGA ATTTCAGTTC TCCCTGGTGG ATTCTGAGTA CGATGAGACC 
CTCGCCTTTC CTGACTCGGT GACCATCCTA TCCAACGTGG GCGACAAGCC GGTGCTGGTG 
GAGCGCAAGG AGACGGACGA CGATGAGGAG GAGTTTGACG ACGAGGAAAA CAACAGTGTA 
GTCCTGCGAC ACGAAATACC ATTCACCAGC ATATACGGGC CGAGCGTCAA GTTCAACTCG 
TTTCAGCGCA AGGTCTTCAT TCCCGGACGT GAGATTCATG TCCGGATCGT CGATACGGAA 
CGCAGCGTCA CCACCCATCT GCTAAATCCC AACCTATACA CAATCGAGCT GACCCATGGT 
CCCTTCAAAT GGACGATCAA GCGGCGCTAC AAGCACTTTA ACTCGCTGCA CCAGCAGCTC 
AGCTTTTTCC GCACCTCGCT CAACATTCCC TTTCCCAGTC GCAGTCACAA GGAGAAGCGT 
ACCACGCTGA AGGCCACTGC CAGAGAGATG GCTGACGAGT CCACTTTGAA GGACCTTCCT 
TCCCACACCA AGGTCAAGCA GACTAGCACA CCGCTGAGGA CTGACGGCAG GAGCAGCAAA 
ATCGCCAGCA GTAACGGCAA CAATGCCATG GCTATCCTAA GTCCCAATCA CAGCTCCATT 
CTGGCGGGTT TTACGCCGCG ACGCATTCAA AAGAAGCGCA AAAAAAAGAA GAAACGGAAG 
CTACCGCGGT TCCCCAATCG TCCTGAGAGT CTGGTCACCG TGGAGAACCT GAGCGTCAGG 
ATCAAGCAGC TGGAGGACTA CTTGTACAAC CTGCTGAACA TAAGTTTGTA CCGATCCCAC 
CACGAAACGC TAAACTTCGT GGAGGCGTCC AATGTGTCCT TCGTTCCGGG AATGGGCATT 
AAGGGCAAGG AAGGATTGAT TTTAAAGCGA ACTGGATCAA CGAGACCGGG GCAAGCGGGA 
TGCAATTTCT TCGGCTGCTT TCAAAAGAAC TGCTGCGTGC GCTGCAACTA CTTTTGCTCC 
GACGTAGTGT GCGGCACGTG GCGGAACCGA TGGTTCTTCG TAAAAGAGAC CTGCTTCGGC 
TACATCCGCC CAACAGACGG CAGCATCCGG GCAGTGATCC TTTTCGATCA GGGCTTCGAC 
GTGTCCACGG GTATCTACCA GACGGGAATG CGCAAGGGCT TGCAGGTGCT GACGAACAAC 
CGTCACATTG TGCTCAAGTG CTGGACACGG CGCAAGTGTA AGGAGTGGAT GCAGTACCTC 
AAGAACATGG CCAACTCGTA TGCGCGCGAC TTCACCCTGC CCAATCCGCA CATGGCCTTC 
GCACCGATGC GCGCCAACAG CCATGCCACG TGGTATGTAG ACGGCGCCCA GTACATGTCA 
GCCGTGGCCG ACGGCTTGGA GGCAGCCCTC GAGGAGATCT ACATTGCCGA CTGGTGGCTC 
AGCCCCGAGA TTTACATGAA GCGACCCGCC CTCGACGGAG ACTACTGGCG TCTGGACAAG 
ATCTTGTTGC GAAAAGCCGA ACAGGGCGTG CGTATCTTCG TGCTGCTATA CAAGGAGGTC 
GAAATGGCTC TTGGCATCAA CAGCTACTAC AGCAAGTCCA CGCTGGCCAA GCACGAAAAC 
ATCAAAGTCA TGCGTCATCC GGACCACGCT AGAGGTGGTA TTTTGCTGTG GGCCCATCAC 
GAAAAGATCG TCGTCATCGA TCAGACCTAT GCCTTCATGG GAGGTATTGA TTTATGCTAT 
GGGCGTTGGG ACGACCACCA TCATCGGCTC ACGGATCTGG GAAGCATATC CACGGCATCT 
TTTTCTGGCA GCGCGCGTCG AACGCCGAGC CTATACTTCA CCAAAGACGA CACGGACTCA 
GCTTTCGGAT CACGAAAGTC GTCGCGGAAT GCCCACTACG ATATGTCCGC CAAGGAGAGG 
CCACCGTCTT CACCCCCGCA GGAGCCCAGT ACTAGCATAG AGTTGAAAAC TCTAAAGCCA 
GGTGATCGCC TGCTTATACC CTCTACGCTC GTTTCGAGTC CGGGCGACAC TCCCGCTGAA 
TCGGGAATCG CTTTAGAGGG AATGAAACTC AACACCCCTG AAATGGAGCG CAAGAACGTA 
CTCGATCGCC TGAAGAACAA CGCGATGAAG GGCGCCCGCA TGGGCAAGGA CTTTATGCAC 
CGACTAACAG CCACGGAGGC GGAGGAAAAA TCAGCCGAGG TGTACACTAT CGAGTCCGAG 
GATGTCACGG ACCACGAAGT CAACCCTAAC ATGGCTTCAG GTGGGGGGGA GGTGGCAATT 
ACCACTAGCA GCACACAAAT ACTCAGTGAG TTCTGCGGCC AGGCCAAGTA CTGGTTCGGC 
AAGGATTACT CCAACTTTAT ACTTAAGGAC TGGATGAACC TAAACTCGCC GTTCGTGGAT 
ATCATAGATC GAACAACAAC ACCGCGGATG CCCTGGCACG ACGTAGGTCT GTGTGTGGTG 
GGTACTTCCG CTAGGGATGT GGCCCGCCAC TTCATCCAGC GCTGGAACGC CATGAAGTTG 
GAGAAACTGC GCGAGAACAC AAGATTCCCC TATCTGATGC CAAAGAGCTA TCACCAAGTG 
AGGCTGAATC CGACCCTGCA GCAAAACCGT CAGCAGCGGG TCACGTGCCA GCTACTTCGG 
AGCGTCTCCG CCTGGAGCTG CGGCTTTATA GAGGCGGATC TGGTGGAGCA GAGCATCCAC 
GATGCCTACA TCCAGACGAT CACCAAGGCG CAGCACTACG TGTACATCGA AAACCAATTT 
TTCATCACCA TGCAGTTGGG CATGGGTGTG CCAGGCGCCC ATAACAATGT GCGGAATCAA 
ATCGGGGAGA CACTCTTTAA GCGGATTGTA CGGGCGCACA AGGAACGTAA GCCTTTCCGA 
GTTTATGTGA TTATGCCGCT CCTTCCGGGC TTCGAGGGCG ACGTGGGTGG CAGTACTGGG 
ATAGCAGTCA GAGCCATTAC GCACTGGAAC TATGCATCCA TTTCCAGGGG ACGCTCCGCA 
ATTTTGAACC GACTGCAGGA GATGGGTATT GCCAATCCGC AAAACTACAT CTCATTCCAC 
AGCCTGCGCA ACCATTCCTT CTTGAACAAC ACACCCATAA CGGAGCTGAT ATATGTCCAC 
TCAAAGCTCC TGATAGCCGA CGATCGTGTG GTGATCTGTG GTTCGGCGAA CATTAACGAT 
CGCTCAATGA TCGGGAAGCG GGACTCCGAG ATAGCGGCTA TCATTATGGA TGAGGAGTTT 
GAGGACGGAC GCATGAATGG CAAGAAGTAT CCGAGCGGAG TGTTTGCCGG TCGCCTTCGA 
AAATATCTTT TTAAAGAACA CTTGGGCCTC CTGGAAAGCG AAGGTTCCAG TCGGTCCGAC 
CTGGACATTA GTGATCCTGT TTGCGACAAG TTTTGGCACG GCACCTGGCG TAGGATTTCA 
ACCCAGAACA CTGAGATTTA CGACGAGGTC TTTAAGTGCA TCCCCACAGA CTTTGTAAAG 
ACCTTTGCCA GCCTTCGCAA ATACCAGGAG GAGCCGCCGC TTGCCAAGAC CGACCCAGAG 
CTAGCTGCCA ACAGGGCCAA CGACATTCAG GGTTACCTGG TCAACCTGCC ATTGGACTTC 
CTGAACAAGG AGGTCCTTAC GCCGCCTGGA ACTAGTAAGG AAGGGCTAAT TCCTACCTCT 
GTATGGACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC6540960 ( XM_026979099.1 ) cDNA ORF clone, Drosophila erecta -> XP_026834900.1 Drosophila erecta phospholipase D2 (LOC6540960), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODi218997
Clone ID Related Accession (Same CDS sequence)	XM_026979099.1
Accession Version	XM_026979099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	phospholipase D2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825200.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG CCAGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTCAG CAACTCGGAC 
GACGAACGCG GCAGCGGGGA AGAGGACGCG TCGGGGGAGA ACTCCAACAA TGATGAGGGG 
GAGGTGTTCC CGGACTGCAC GGACGAAGCG GCAGAGGAGC ATCAAAACCG CTGCTTGCCA 
GAATTTCAGT TCTCCCTGGT GGATTCTGAG TACGATGAGA CCCTCGCCTT TCCTGACTCG 
GTGACCATCC TATCCAACGT GGGCGACAAG CCGGTGCTGG TGGAGCGCAA GGAGACGGAC 
GACGATGAGG AGGAGTTTGA CGACGAGGAA AACAACAGTG TAGTCCTGCG ACACGAAATA 
CCATTCACCA GCATATACGG GCCGAGCGTC AAGTTCAACT CGTTTCAGCG CAAGGTCTTC 
ATTCCCGGAC GTGAGATTCA TGTCCGGATC GTCGATACGG AACGCAGCGT CACCACCCAT 
CTGCTAAATC CCAACCTATA CACAATCGAG CTGACCCATG GTCCCTTCAA ATGGACGATC 
AAGCGGCGCT ACAAGCACTT TAACTCGCTG CACCAGCAGC TCAGCTTTTT CCGCACCTCG 
CTCAACATTC CCTTTCCCAG TCGCAGTCAC AAGGAGAAGC GTACCACGCT GAAGGCCACT 
GCCAGAGAGA TGGCTGACGA GTCCACTTTG AAGGACCTTC CTTCCCACAC CAAGGTCAAG 
CAGACTAGCA CACCGCTGAG GACTGACGGC AGGAGCAGCA AAATCGCCAG CAGTAACGGC 
AACAATGCCA TGGCTATCCT AAGTCCCAAT CACAGCTCCA TTCTGGCGGG TTTTACGCCG 
CGACGCATTC AAAAGAAGCG CAAAAAAAAG AAGAAACGGA AGCTACCGCG GTTCCCCAAT 
CGTCCTGAGA GTCTGGTCAC CGTGGAGAAC CTGAGCGTCA GGATCAAGCA GCTGGAGGAC 
TACTTGTACA ACCTGCTGAA CATAAGTTTG TACCGATCCC ACCACGAAAC GCTAAACTTC 
GTGGAGGCGT CCAATGTGTC CTTCGTTCCG GGAATGGGCA TTAAGGGCAA GGAAGGATTG 
ATTTTAAAGC GAACTGGATC AACGAGACCG GGGCAAGCGG GATGCAATTT CTTCGGCTGC 
TTTCAAAAGA ACTGCTGCGT GCGCTGCAAC TACTTTTGCT CCGACGTAGT GTGCGGCACG 
TGGCGGAACC GATGGTTCTT CGTAAAAGAG ACCTGCTTCG GCTACATCCG CCCAACAGAC 
GGCAGCATCC GGGCAGTGAT CCTTTTCGAT CAGGGCTTCG ACGTGTCCAC GGGTATCTAC 
CAGACGGGAA TGCGCAAGGG CTTGCAGGTG CTGACGAACA ACCGTCACAT TGTGCTCAAG 
TGCTGGACAC GGCGCAAGTG TAAGGAGTGG ATGCAGTACC TCAAGAACAT GGCCAACTCG 
TATGCGCGCG ACTTCACCCT GCCCAATCCG CACATGGCCT TCGCACCGAT GCGCGCCAAC 
AGCCATGCCA CGTGGTATGT AGACGGCGCC CAGTACATGT CAGCCGTGGC CGACGGCTTG 
GAGGCAGCCC TCGAGGAGAT CTACATTGCC GACTGGTGGC TCAGCCCCGA GATTTACATG 
AAGCGACCCG CCCTCGACGG AGACTACTGG CGTCTGGACA AGATCTTGTT GCGAAAAGCC 
GAACAGGGCG TGCGTATCTT CGTGCTGCTA TACAAGGAGG TCGAAATGGC TCTTGGCATC 
AACAGCTACT ACAGCAAGTC CACGCTGGCC AAGCACGAAA ACATCAAAGT CATGCGTCAT 
CCGGACCACG CTAGAGGTGG TATTTTGCTG TGGGCCCATC ACGAAAAGAT CGTCGTCATC 
GATCAGACCT ATGCCTTCAT GGGAGGTATT GATTTATGCT ATGGGCGTTG GGACGACCAC 
CATCATCGGC TCACGGATCT GGGAAGCATA TCCACGGCAT CTTTTTCTGG CAGCGCGCGT 
CGAACGCCGA GCCTATACTT CACCAAAGAC GACACGGACT CAGCTTTCGG ATCACGAAAG 
TCGTCGCGGA ATGCCCACTA CGATATGTCC GCCAAGGAGA GGCCACCGTC TTCACCCCCG 
CAGGAGCCCA GTACTAGCAT AGAGTTGAAA ACTCTAAAGC CAGGTGATCG CCTGCTTATA 
CCCTCTACGC TCGTTTCGAG TCCGGGCGAC ACTCCCGCTG AATCGGGAAT CGCTTTAGAG 
GGAATGAAAC TCAACACCCC TGAAATGGAG CGCAAGAACG TACTCGATCG CCTGAAGAAC 
AACGCGATGA AGGGCGCCCG CATGGGCAAG GACTTTATGC ACCGACTAAC AGCCACGGAG 
GCGGAGGAAA AATCAGCCGA GGTGTACACT ATCGAGTCCG AGGATGTCAC GGACCACGAA 
GTCAACCCTA ACATGGCTTC AGGTGGGGGG GAGGTGGCAA TTACCACTAG CAGCACACAA 
ATACTCAGTG AGTTCTGCGG CCAGGCCAAG TACTGGTTCG GCAAGGATTA CTCCAACTTT 
ATACTTAAGG ACTGGATGAA CCTAAACTCG CCGTTCGTGG ATATCATAGA TCGAACAACA 
ACACCGCGGA TGCCCTGGCA CGACGTAGGT CTGTGTGTGG TGGGTACTTC CGCTAGGGAT 
GTGGCCCGCC ACTTCATCCA GCGCTGGAAC GCCATGAAGT TGGAGAAACT GCGCGAGAAC 
ACAAGATTCC CCTATCTGAT GCCAAAGAGC TATCACCAAG TGAGGCTGAA TCCGACCCTG 
CAGCAAAACC GTCAGCAGCG GGTCACGTGC CAGCTACTTC GGAGCGTCTC CGCCTGGAGC 
TGCGGCTTTA TAGAGGCGGA TCTGGTGGAG CAGAGCATCC ACGATGCCTA CATCCAGACG 
ATCACCAAGG CGCAGCACTA CGTGTACATC GAAAACCAAT TTTTCATCAC CATGCAGTTG 
GGCATGGGTG TGCCAGGCGC CCATAACAAT GTGCGGAATC AAATCGGGGA GACACTCTTT 
AAGCGGATTG TACGGGCGCA CAAGGAACGT AAGCCTTTCC GAGTTTATGT GATTATGCCG 
CTCCTTCCGG GCTTCGAGGG CGACGTGGGT GGCAGTACTG GGATAGCAGT CAGAGCCATT 
ACGCACTGGA ACTATGCATC CATTTCCAGG GGACGCTCCG CAATTTTGAA CCGACTGCAG 
GAGATGGGTA TTGCCAATCC GCAAAACTAC ATCTCATTCC ACAGCCTGCG CAACCATTCC 
TTCTTGAACA ACACACCCAT AACGGAGCTG ATATATGTCC ACTCAAAGCT CCTGATAGCC 
GACGATCGTG TGGTGATCTG TGGTTCGGCG AACATTAACG ATCGCTCAAT GATCGGGAAG 
CGGGACTCCG AGATAGCGGC TATCATTATG GATGAGGAGT TTGAGGACGG ACGCATGAAT 
GGCAAGAAGT ATCCGAGCGG AGTGTTTGCC GGTCGCCTTC GAAAATATCT TTTTAAAGAA 
CACTTGGGCC TCCTGGAAAG CGAAGGTTCC AGTCGGTCCG ACCTGGACAT TAGTGATCCT 
GTTTGCGACA AGTTTTGGCA CGGCACCTGG CGTAGGATTT CAACCCAGAA CACTGAGATT 
TACGACGAGG TCTTTAAGTG CATCCCCACA GACTTTGTAA AGACCTTTGC CAGCCTTCGC 
AAATACCAGG AGGAGCCGCC GCTTGCCAAG ACCGACCCAG AGCTAGCTGC CAACAGGGCC 
AACGACATTC AGGGTTACCT GGTCAACCTG CCATTGGACT TCCTGAACAA GGAGGTCCTT 
ACGCCGCCTG GAACTAGTAA GGAAGGGCTA ATTCCTACCT CTGTATGGAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026834900.1
CDS	996..4829
Translation	MADASTVGGALPPYALSNSDDERGSGEEDASGENSNNDEGEVFPDCTDEAAEEHQNRCLPEFQFSLVDSEYDETLAFPDSVTILSNVGDKPVLVERKETDDDEEEFDDEENNSVVLRHEIPFTSIYGPSVKFNSFQRKVFIPGREIHVRIVDTERSVTTHLLNPNLYTIELTHGPFKWTIKRRYKHFNSLHQQLSFFRTSLNIPFPSRSHKEKRTTLKATAREMADESTLKDLPSHTKVKQTSTPLRTDGRSSKIASSNGNNAMAILSPNHSSILAGFTPRRIQKKRKKKKKRKLPRFPNRPESLVTVENLSVRIKQLEDYLYNLLNISLYRSHHETLNFVEASNVSFVPGMGIKGKEGLILKRTGSTRPGQAGCNFFGCFQKNCCVRCNYFCSDVVCGTWRNRWFFVKETCFGYIRPTDGSIRAVILFDQGFDVSTGIYQTGMRKGLQVLTNNRHIVLKCWTRRKCKEWMQYLKNMANSYARDFTLPNPHMAFAPMRANSHATWYVDGAQYMSAVADGLEAALEEIYIADWWLSPEIYMKRPALDGDYWRLDKILLRKAEQGVRIFVLLYKEVEMALGINSYYSKSTLAKHENIKVMRHPDHARGGILLWAHHEKIVVIDQTYAFMGGIDLCYGRWDDHHHRLTDLGSISTASFSGSARRTPSLYFTKDDTDSAFGSRKSSRNAHYDMSAKERPPSSPPQEPSTSIELKTLKPGDRLLIPSTLVSSPGDTPAESGIALEGMKLNTPEMERKNVLDRLKNNAMKGARMGKDFMHRLTATEAEEKSAEVYTIESEDVTDHEVNPNMASGGGEVAITTSSTQILSEFCGQAKYWFGKDYSNFILKDWMNLNSPFVDIIDRTTTPRMPWHDVGLCVVGTSARDVARHFIQRWNAMKLEKLRENTRFPYLMPKSYHQVRLNPTLQQNRQQRVTCQLLRSVSAWSCGFIEADLVEQSIHDAYIQTITKAQHYVYIENQFFITMQLGMGVPGAHNNVRNQIGETLFKRIVRAHKERKPFRVYVIMPLLPGFEGDVGGSTGIAVRAITHWNYASISRGRSAILNRLQEMGIANPQNYISFHSLRNHSFLNNTPITELIYVHSKLLIADDRVVICGSANINDRSMIGKRDSEIAAIIMDEEFEDGRMNGKKYPSGVFAGRLRKYLFKEHLGLLESEGSSRSDLDISDPVCDKFWHGTWRRISTQNTEIYDEVFKCIPTDFVKTFASLRKYQEEPPLAKTDPELAANRANDIQGYLVNLPLDFLNKEVLTPPGTSKEGLIPTSVWT

Target ORF information:

RefSeq Version	XM_026979099.1
Organism	Drosophila erecta
Definition	Drosophila erecta phospholipase D2 (LOC6540960), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026979099.1

ORF Insert Sequence:

ATGGCAGATG CCAGCACCGT TGGAGGCGCC TTGCCGCCCT ACGCACTCAG CAACTCGGAC 
GACGAACGCG GCAGCGGGGA AGAGGACGCG TCGGGGGAGA ACTCCAACAA TGATGAGGGG 
GAGGTGTTCC CGGACTGCAC GGACGAAGCG GCAGAGGAGC ATCAAAACCG CTGCTTGCCA 
GAATTTCAGT TCTCCCTGGT GGATTCTGAG TACGATGAGA CCCTCGCCTT TCCTGACTCG 
GTGACCATCC TATCCAACGT GGGCGACAAG CCGGTGCTGG TGGAGCGCAA GGAGACGGAC 
GACGATGAGG AGGAGTTTGA CGACGAGGAA AACAACAGTG TAGTCCTGCG ACACGAAATA 
CCATTCACCA GCATATACGG GCCGAGCGTC AAGTTCAACT CGTTTCAGCG CAAGGTCTTC 
ATTCCCGGAC GTGAGATTCA TGTCCGGATC GTCGATACGG AACGCAGCGT CACCACCCAT 
CTGCTAAATC CCAACCTATA CACAATCGAG CTGACCCATG GTCCCTTCAA ATGGACGATC 
AAGCGGCGCT ACAAGCACTT TAACTCGCTG CACCAGCAGC TCAGCTTTTT CCGCACCTCG 
CTCAACATTC CCTTTCCCAG TCGCAGTCAC AAGGAGAAGC GTACCACGCT GAAGGCCACT 
GCCAGAGAGA TGGCTGACGA GTCCACTTTG AAGGACCTTC CTTCCCACAC CAAGGTCAAG 
CAGACTAGCA CACCGCTGAG GACTGACGGC AGGAGCAGCA AAATCGCCAG CAGTAACGGC 
AACAATGCCA TGGCTATCCT AAGTCCCAAT CACAGCTCCA TTCTGGCGGG TTTTACGCCG 
CGACGCATTC AAAAGAAGCG CAAAAAAAAG AAGAAACGGA AGCTACCGCG GTTCCCCAAT 
CGTCCTGAGA GTCTGGTCAC CGTGGAGAAC CTGAGCGTCA GGATCAAGCA GCTGGAGGAC 
TACTTGTACA ACCTGCTGAA CATAAGTTTG TACCGATCCC ACCACGAAAC GCTAAACTTC 
GTGGAGGCGT CCAATGTGTC CTTCGTTCCG GGAATGGGCA TTAAGGGCAA GGAAGGATTG 
ATTTTAAAGC GAACTGGATC AACGAGACCG GGGCAAGCGG GATGCAATTT CTTCGGCTGC 
TTTCAAAAGA ACTGCTGCGT GCGCTGCAAC TACTTTTGCT CCGACGTAGT GTGCGGCACG 
TGGCGGAACC GATGGTTCTT CGTAAAAGAG ACCTGCTTCG GCTACATCCG CCCAACAGAC 
GGCAGCATCC GGGCAGTGAT CCTTTTCGAT CAGGGCTTCG ACGTGTCCAC GGGTATCTAC 
CAGACGGGAA TGCGCAAGGG CTTGCAGGTG CTGACGAACA ACCGTCACAT TGTGCTCAAG 
TGCTGGACAC GGCGCAAGTG TAAGGAGTGG ATGCAGTACC TCAAGAACAT GGCCAACTCG 
TATGCGCGCG ACTTCACCCT GCCCAATCCG CACATGGCCT TCGCACCGAT GCGCGCCAAC 
AGCCATGCCA CGTGGTATGT AGACGGCGCC CAGTACATGT CAGCCGTGGC CGACGGCTTG 
GAGGCAGCCC TCGAGGAGAT CTACATTGCC GACTGGTGGC TCAGCCCCGA GATTTACATG 
AAGCGACCCG CCCTCGACGG AGACTACTGG CGTCTGGACA AGATCTTGTT GCGAAAAGCC 
GAACAGGGCG TGCGTATCTT CGTGCTGCTA TACAAGGAGG TCGAAATGGC TCTTGGCATC 
AACAGCTACT ACAGCAAGTC CACGCTGGCC AAGCACGAAA ACATCAAAGT CATGCGTCAT 
CCGGACCACG CTAGAGGTGG TATTTTGCTG TGGGCCCATC ACGAAAAGAT CGTCGTCATC 
GATCAGACCT ATGCCTTCAT GGGAGGTATT GATTTATGCT ATGGGCGTTG GGACGACCAC 
CATCATCGGC TCACGGATCT GGGAAGCATA TCCACGGCAT CTTTTTCTGG CAGCGCGCGT 
CGAACGCCGA GCCTATACTT CACCAAAGAC GACACGGACT CAGCTTTCGG ATCACGAAAG 
TCGTCGCGGA ATGCCCACTA CGATATGTCC GCCAAGGAGA GGCCACCGTC TTCACCCCCG 
CAGGAGCCCA GTACTAGCAT AGAGTTGAAA ACTCTAAAGC CAGGTGATCG CCTGCTTATA 
CCCTCTACGC TCGTTTCGAG TCCGGGCGAC ACTCCCGCTG AATCGGGAAT CGCTTTAGAG 
GGAATGAAAC TCAACACCCC TGAAATGGAG CGCAAGAACG TACTCGATCG CCTGAAGAAC 
AACGCGATGA AGGGCGCCCG CATGGGCAAG GACTTTATGC ACCGACTAAC AGCCACGGAG 
GCGGAGGAAA AATCAGCCGA GGTGTACACT ATCGAGTCCG AGGATGTCAC GGACCACGAA 
GTCAACCCTA ACATGGCTTC AGGTGGGGGG GAGGTGGCAA TTACCACTAG CAGCACACAA 
ATACTCAGTG AGTTCTGCGG CCAGGCCAAG TACTGGTTCG GCAAGGATTA CTCCAACTTT 
ATACTTAAGG ACTGGATGAA CCTAAACTCG CCGTTCGTGG ATATCATAGA TCGAACAACA 
ACACCGCGGA TGCCCTGGCA CGACGTAGGT CTGTGTGTGG TGGGTACTTC CGCTAGGGAT 
GTGGCCCGCC ACTTCATCCA GCGCTGGAAC GCCATGAAGT TGGAGAAACT GCGCGAGAAC 
ACAAGATTCC CCTATCTGAT GCCAAAGAGC TATCACCAAG TGAGGCTGAA TCCGACCCTG 
CAGCAAAACC GTCAGCAGCG GGTCACGTGC CAGCTACTTC GGAGCGTCTC CGCCTGGAGC 
TGCGGCTTTA TAGAGGCGGA TCTGGTGGAG CAGAGCATCC ACGATGCCTA CATCCAGACG 
ATCACCAAGG CGCAGCACTA CGTGTACATC GAAAACCAAT TTTTCATCAC CATGCAGTTG 
GGCATGGGTG TGCCAGGCGC CCATAACAAT GTGCGGAATC AAATCGGGGA GACACTCTTT 
AAGCGGATTG TACGGGCGCA CAAGGAACGT AAGCCTTTCC GAGTTTATGT GATTATGCCG 
CTCCTTCCGG GCTTCGAGGG CGACGTGGGT GGCAGTACTG GGATAGCAGT CAGAGCCATT 
ACGCACTGGA ACTATGCATC CATTTCCAGG GGACGCTCCG CAATTTTGAA CCGACTGCAG 
GAGATGGGTA TTGCCAATCC GCAAAACTAC ATCTCATTCC ACAGCCTGCG CAACCATTCC 
TTCTTGAACA ACACACCCAT AACGGAGCTG ATATATGTCC ACTCAAAGCT CCTGATAGCC 
GACGATCGTG TGGTGATCTG TGGTTCGGCG AACATTAACG ATCGCTCAAT GATCGGGAAG 
CGGGACTCCG AGATAGCGGC TATCATTATG GATGAGGAGT TTGAGGACGG ACGCATGAAT 
GGCAAGAAGT ATCCGAGCGG AGTGTTTGCC GGTCGCCTTC GAAAATATCT TTTTAAAGAA 
CACTTGGGCC TCCTGGAAAG CGAAGGTTCC AGTCGGTCCG ACCTGGACAT TAGTGATCCT 
GTTTGCGACA AGTTTTGGCA CGGCACCTGG CGTAGGATTT CAACCCAGAA CACTGAGATT 
TACGACGAGG TCTTTAAGTG CATCCCCACA GACTTTGTAA AGACCTTTGC CAGCCTTCGC 
AAATACCAGG AGGAGCCGCC GCTTGCCAAG ACCGACCCAG AGCTAGCTGC CAACAGGGCC 
AACGACATTC AGGGTTACCT GGTCAACCTG CCATTGGACT TCCTGAACAA GGAGGTCCTT 
ACGCCGCCTG GAACTAGTAA GGAAGGGCTA ATTCCTACCT CTGTATGGAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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LOC6540960 cDNA ORF clone, Drosophila erecta

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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