BRAFLDRAFT_91658 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_91658
Entrez Gene ID	7219466
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002606843.1	XP_002606889.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_91658 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_91658 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb06354	XM_002606843.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_91658 ( XM_002606843.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002606889.1 Branchiostoma floridae hypothetical protein, mRNA.

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CloneID	OBb06354
Clone ID Related Accession (Same CDS sequence)	XM_002606843.1
Accession Version	XM_002606843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101528). COMPLETENESS: incomplete on both ends.

ATGGAACAGT CATTCTTTGC TGCAGCTAAT GATTTGGACA ATGTCCTTGA TGAGTTTGAA 
AGAAATGAAG ATTTCCAGCT TGGGCAGAGT GTAGACTTTG CTACAGCCAA GCCAACAACA 
CCGGCTCAAA CTCCTCAAAC CTCATCAGCA CCCAGCCATC GGGTCCCCAA CAGCAGCCTG 
GCAGGCTTCT ACCCTGTCAC AACAGGAGAT GGGGCAAGGG CTAGTCCCCA GTACCACCCT 
GCTGCAATGC TGCAGCATTC CACAGGGGGC GCTGTGTACA ATGGTGGGAA CTACAGTGGT 
GTGGACGGTG GGATGGCAAG CATTGGTGCT CAGAGTCAAG TGAAAGGATA TCCAGTTCAG 
TCTAATTTGA ATGGCCCAAC GGGACAGCTT TTGGGGACAT CCACTGCAAG TAACTACCAA 
ACGCAAGCAG GACTTGTGAA CTATCAGGCA GGCACCTTCC ATGGGTACCA AGGACAGCCA 
GTAATGAACA CACATCAAGG TCAAGTAGCT GTGAATAATC TTCAGCAACA GCAAGCTTTA 
AGCAGCTACC AAACAGTGAC AGGACAGAAC AGCTATTCCG TACAGAGAGG GGTGACTAGC 
TTTCCAACAC AGCCACCAAT GGGCACTTAC CAACAACATG TCGCAAACAG CAGTTTCCAG 
TCCCAAGCAG GGTTGAACAG CTATCCAGCA GCTGTAGGTC TTGCCTCTCA CCCTGTACCA 
GGTGTGTCAC AACATCAGAC AGTTGGACAA CCAAACCTCC AGCTGCTTGG ATCAATCCAA 
GACAGCCAGG TTCAGCCCTT GGTACCACCA CTTAACACCA AACCACCAAT GTCACTCCAC 
GGTGGGGCAT ACCCCAGTGT TGGACGAAGC TCATCCCCTC CAAAACCAGT GAACATAACA 
ACCGCTGAAG CTCAGGTCCC AAACAGTGCA GCAATGGTTA AATCTGGCCT GTATCCTATG 
TCAGCAGGAC TGACACAAGG TGTTAGTAGC ACTCCGAGTA GCACAATAGT TGGTGGTGGG 
CAAGGGGTGT ATCCATCTGT GCCAGCCTCA ACTGGTCTGT ACCCAGCAGC TGATCTAACA 
CAACCCAGCA TAGATCCAAA TTTTGCTGGA CTAGGAGGTA AAGGCAATGG GCTTTACCCA 
CAAACAGCAG CATTAAATCA GATGGCCAAT TTACCGACCA AGGGCAATGT TGGTATTACT 
GGTAGTGTGA CAGGTGGTGG GTTGTATCCT CAGACTACCG GCCTCCAGGC GACTTTAGGA 
ACTGATAATG TAGCTTCGAT ATTGCCTGGC ATTGGTGGAG GGGAGGCTAC TATGTATCCC 
AGCATCGGAC TGGCGAAGCC TGTAACAACA AATATCCAAG CTCAAGCAAC TGCTGATGAA 
TTAGTCAAAT ATATGATGAC TTCCCAGATC CTTAAAAAAG ACAGGGACAC ACAGCCGCCA 
CTGTCCACAG GTAATCCAAA TGTCAGCTTT GAATCTGCAC CAATCACTGT AAACCCAACG 
GGAGACAGAG GGCTGGAGGC AGCAGCTTCT TTAGATCTCA ATCTCGGAGG GAAACCAGCT 
GGAGAAACAC AAATGCCCAG CCTGCCAATG TCAAGAAGTG GTAAAACAGA TCAAGATAAC 
ATAGTCCAGG ACATGGGTGA AAAAAGGAAC GTTACAGCAC CAAGTACAGG TCTGAACATG 
CTGGAAGCCA ACGAGAAGGT AAACAAAGGT GATACTGTAC AAGGAGAGGC ACAGAAAAAT 
AGTCAGGAAA ACAACATTGT TGGGAACAAT GCTACTAATA AGGACATTAA AGTTGAGAAT 
AGTCAGTCAA CAGTCAATGT AAGAGATAGC TTTTCCAATG TACAACAAAC TGAAGTGTTA 
TCTGAAAATA AGGCAATGAT AGCACCTGAA CAAGTAGCAC ATAGCAGTCA AAGAACTGAA 
GAAAGTGGCG TTGTGTTGGG AGATGATAAA CCAAGTGAGC CTGTTCATTC CAATGATTTA 
ATGGATAGTG TTGGAAATAC AAATATGACT CAGAAATCCA GTATGGCATC TACACCATTG 
AAAGACAGTG ACAATGTGCC TGAGGGTGAT ATTGCTCGAG GGAATGGTTT AAAACAGAAG 
AAAGAAGTAG AGCATACTGC CACACAGGGT GTGAAAATTG CAGAAAATGC AGATGTCGTA 
AGCCAAGAAA AGGGTGGACC TCAAATGATA GGCTTCACTC CAGAGGAGGA GGTAGATGAA 
GCATTTGATG ATGCAGAAAT TGATGCATAC CTCGGGACGG AGGGAGTTGG CCTTGGAAAC 
TCTGGGAATG AGAGCATGAT GGAGGCAGGC AGAGAGGATG CTGGGATTCC TGAATCAGAT 
GCAGTATTGG AACAAGTCAT GGAAAGGTCT AGCCAGGAAG GGTCAGTTAC TGGTGTTTCA 
CAGCTACCAG TCACTATTTT CCTTCCAACT TCTGGTGCTG AGAGCAAAGA ACAAAAGTCA 
GACCAGGTTT TGAACAACAG TGAAGGGAAA ACTTACCCCA AAGATGGGGT CACAGCAGTT 
GGAGAACTCT CTGATGCTGA TCCTTTTTTC AGTGCACAGA CAAGTACCAG TCCAACTGAT 
ACAATAACAT CAATGGAAGA AGAAGAGGCT GTTACTGCAG CAGCATCAGT TGATAGTAAT 
ATACTGGATG GTAGGACTAT TGAACAAAAA GCTGGAAGCT CTGAGACTTT GATGAAGGGC 
GACTTGGAAG AAAGATTTCA GATTGAATCT AGAAAGCTTT CTGATATCAT TGGAAACAAT 
AGTAACATTG AGGACAGCAT TGCAGGAGCA CACGATGATG AGGATAGAAC TATCACATCA 
TTACAGGGGA CAGTCACACT TGGAGATAAT CCTGGTCACA TCCAGGAACA ACCCAGTCAC 
ACCCAGCAAC CACCTGGTTA CTCCCAACAA CCATCCGGTC ACACCCAGCA ACCACATGAT 
CATACCCAGC AACCACCTGG TTACACCCAG CAACCAAGTC AGCCAGAGGT AGACAGAAGT 
ATGGAGTCAG CCGTGGCAAA CATGGCGATC AGCGGACAAA TGGAACAGGC TCAACAAGAA 
CAACCATTCC CAGATTACAG CGAGACGTGG GACACCAGAC AGGCCCCGCA CCAGGACATG 
CCCCAGGACG GAGTCAGGAT GCCCCCACAC CCTCCCCTGG GAGTGGCCTC CCCGGAGGAT 
GTCGGGTTCC CACCCAATGT GGAGATGTTC AGGGATGACC TCAGTTCAGT TCTGCCATCA 
GATATCGGCT GGGAGGCCCC CGTATGGGTC CCCGACGAGG ATGCTCCAAA CTGCATGAAG 
TGTGAGTCCA AGTTCACGTT CACCAAGCGG CGGCACCACT GCAGGGCGTG CGGGAAGGTG 
CTGTGCTCGG CCTGCTGTAG TCAGAAGGCA CGGCTGGCGT ACATGGACAA CAAGTCAGCC 
CGCGTCTGCT CAACGTGCTA CAACATTCTA CAGAGAGCCC AGGCCAGAGG TCAGATTGCA 
GGCATCAGCC AGGTGACCCC ATCTTCAGGT CAGGCTTCCC CCAACCCTAA CAACCCCATG 
GAGTACTGCT CCACTCTGCC ACCTATGCAG CAGGCACAGG AAGCACTGCA GAGGCCACCC 
CCCAGTGTAC TGGTCCCTGT GGGTGGAAGG CCCCAAGGTG TTGCCCAGAG AGAAGGACGG 
CACGTGAGAT TTTCTGACGG AAGCATCCCC GGTGGGGACG GGACCTCCGA CTCCTCTACC 
CCCTCCACCC CCTCCCACCC TATCACTGCC CCCCAGGGAG CTGCGGCAGT GAGACCCAGG 
GCTGCACCAA ACACATCTCC TCAACATAAG GGGTCCCCCA GTAAAACAGC CGAGACTAGG 
GGTAGACTCA GTCCATCCAA GAACAGGGGG CAACACTCAG AGAGGCAATC AGGAAGTCCT 
CGCCAAGGAG GAAGTCCGAG GAAAACAGGG AGCCAAAGGT CACCCCCAAG GTCACGTCAG 
TCATCAAGGA CAAAGAGTCT AATTCCTGAG GAGGAAGACA GACTGCCTCC AGTCATCTTG 
TCTATAGGAG ACAAAGGAGA CTACGAGGTT GATTCGGAGC CGGACCCAGA CAGAACCCTG 
TCAGAGATAA AGAGGGAGGA GACGGACCCT GTCATGTTCC TGGCTAACCC CAACCTGGTG 
GTGCTGGTCA AGGTCATCAA CTTGTCGTGC TGTGTGAACA GGCTGTGCTG GTGTTTCTCC 
ACACGAGGGA TGCACACGGT CAGTCAGGAC GAGGTGGTCA TCCTGCTGGA GGCCATGCCT 
GATGATGATG TCATCCCCAG GGACATCTTT GCCCACCTGC AAATGGTGTA TGAGGAGGCA 
GGCAAAGGTA ATACAGTGGG AGACCTGGGC CATACCATCT TCAACCAGCC GTTCTTGGGC 
AGCCGTGAGC ATGGAGGTTT CCTGTACATC CAGCCCACCT TCCAGTGCAC CCAGCGCCTC 
ATCCTGCCGG ACCCCCCCTA TGTGGTGGGG GTGCTGCTGC AGAAGTGGGA GACACCCTGG 
GCAAAGGTCT TCCCCATACG CCTCATGCTT AGGCTGGGGG CTGAGTTCAG ATATTACCCC 
TGTCCACTGT TCAGCGTGCG GTTCCGCAAG CCAGTGTACT GTGAGATTGG ACATACCATC 
ATGAACCTAC TGGCTGATTT CCGTAACTAC CAGTACATGT TGGGACAGGT GAGGGGTGTG 
GTCATACTGC TGGAAGACAA GAGAACTCTG GTCAGGATAC CCAGCAACAG ATATGATGAT 
ATGATGAAAG TGCTGAACAA CTGTAATGAG CACGTCCTCT CCTGGGGCTC AAACTTCAGT 
GAGGAGGCGG ACTCCCATCT AGTGTGTATA CAGAACGACG AGGGAGCGTA CCAGACCCAG 
GCTATCAGCA TCCAGAACCG CCAACGGAAG GTCACCGGAG CCAGCTTTAT CGTGTTTAAC 
GGCTCGCTGA AGACAACGGC TGGGCTGAGG GGAAAGTCTA GCATTGTGGA GGATGGAGTG 
ATGGTACAGA TCCTACCTGA GACGATGTCG GAGCTTCGCC AGGCACTGCG GAACATGAAG 
GACTACACCA TCGGCTGTGG GAGCACGGCC TCTGAGGAAC CCGACGAGAC GGTGGAGGTC 
CGCTGGGTAG AGGACGACCG CAAAATGAAC ATTGGAGTCA CAAGTCCAAT TGATGGCCAG 
TCCATGGAGG GCTTGACCAA TATCCATGTG CACAGTGGCA TGGACTTTGC TGGGGAACAG 
AGGACCATTC GCTGGACCGA AGTCTTCTTC CTGCAAACTG AGGACTCTAT GCCCCAGTGT 
GAGCCCATGG AACTTAGCAG GCTGGCGGAG TCCATCGCCA CGGCAACCTG CGTGGCGCTG 
GTGCAGCACC TGGACTCCCT GAGGGAGGCT GGGATGAACA AACTGGGCCT GAGGGTCAAC 
ATAGACAGGG ACGAGGTTGG GTACGTAGCA GGTTCCAAGG GTCAGTTTCT GCCACCCCTG 
TACATGACCG ACCTTGACAA TGAACTCATC CCCGTCATCC ACAACGCAGC AGCGTCCAAT 
CAGGACACAC CCATCAGATT GGAGCTCATC TTCCATATTC TCCACAACGT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002606889.1
CDS	1..5577
Translation	MEQSFFAAANDLDNVLDEFERNEDFQLGQSVDFATAKPTTPAQTPQTSSAPSHRVPNSSLAGFYPVTTGDGARASPQYHPAAMLQHSTGGAVYNGGNYSGVDGGMASIGAQSQVKGYPVQSNLNGPTGQLLGTSTASNYQTQAGLVNYQAGTFHGYQGQPVMNTHQGQVAVNNLQQQQALSSYQTVTGQNSYSVQRGVTSFPTQPPMGTYQQHVANSSFQSQAGLNSYPAAVGLASHPVPGVSQHQTVGQPNLQLLGSIQDSQVQPLVPPLNTKPPMSLHGGAYPSVGRSSSPPKPVNITTAEAQVPNSAAMVKSGLYPMSAGLTQGVSSTPSSTIVGGGQGVYPSVPASTGLYPAADLTQPSIDPNFAGLGGKGNGLYPQTAALNQMANLPTKGNVGITGSVTGGGLYPQTTGLQATLGTDNVASILPGIGGGEATMYPSIGLAKPVTTNIQAQATADELVKYMMTSQILKKDRDTQPPLSTGNPNVSFESAPITVNPTGDRGLEAAASLDLNLGGKPAGETQMPSLPMSRSGKTDQDNIVQDMGEKRNVTAPSTGLNMLEANEKVNKGDTVQGEAQKNSQENNIVGNNATNKDIKVENSQSTVNVRDSFSNVQQTEVLSENKAMIAPEQVAHSSQRTEESGVVLGDDKPSEPVHSNDLMDSVGNTNMTQKSSMASTPLKDSDNVPEGDIARGNGLKQKKEVEHTATQGVKIAENADVVSQEKGGPQMIGFTPEEEVDEAFDDAEIDAYLGTEGVGLGNSGNESMMEAGREDAGIPESDAVLEQVMERSSQEGSVTGVSQLPVTIFLPTSGAESKEQKSDQVLNNSEGKTYPKDGVTAVGELSDADPFFSAQTSTSPTDTITSMEEEEAVTAAASVDSNILDGRTIEQKAGSSETLMKGDLEERFQIESRKLSDIIGNNSNIEDSIAGAHDDEDRTITSLQGTVTLGDNPGHIQEQPSHTQQPPGYSQQPSGHTQQPHDHTQQPPGYTQQPSQPEVDRSMESAVANMAISGQMEQAQQEQPFPDYSETWDTRQAPHQDMPQDGVRMPPHPPLGVASPEDVGFPPNVEMFRDDLSSVLPSDIGWEAPVWVPDEDAPNCMKCESKFTFTKRRHHCRACGKVLCSACCSQKARLAYMDNKSARVCSTCYNILQRAQARGQIAGISQVTPSSGQASPNPNNPMEYCSTLPPMQQAQEALQRPPPSVLVPVGGRPQGVAQREGRHVRFSDGSIPGGDGTSDSSTPSTPSHPITAPQGAAAVRPRAAPNTSPQHKGSPSKTAETRGRLSPSKNRGQHSERQSGSPRQGGSPRKTGSQRSPPRSRQSSRTKSLIPEEEDRLPPVILSIGDKGDYEVDSEPDPDRTLSEIKREETDPVMFLANPNLVVLVKVINLSCCVNRLCWCFSTRGMHTVSQDEVVILLEAMPDDDVIPRDIFAHLQMVYEEAGKGNTVGDLGHTIFNQPFLGSREHGGFLYIQPTFQCTQRLILPDPPYVVGVLLQKWETPWAKVFPIRLMLRLGAEFRYYPCPLFSVRFRKPVYCEIGHTIMNLLADFRNYQYMLGQVRGVVILLEDKRTLVRIPSNRYDDMMKVLNNCNEHVLSWGSNFSEEADSHLVCIQNDEGAYQTQAISIQNRQRKVTGASFIVFNGSLKTTAGLRGKSSIVEDGVMVQILPETMSELRQALRNMKDYTIGCGSTASEEPDETVEVRWVEDDRKMNIGVTSPIDGQSMEGLTNIHVHSGMDFAGEQRTIRWTEVFFLQTEDSMPQCEPMELSRLAESIATATCVALVQHLDSLREAGMNKLGLRVNIDRDEVGYVAGSKGQFLPPLYMTDLDNELIPVIHNAAASNQDTPIRLELIFHILHNVD

Target ORF information:

RefSeq Version	XM_002606843.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002606843.1

ORF Insert Sequence:

ATGGAACAGT CATTCTTTGC TGCAGCTAAT GATTTGGACA ATGTCCTTGA TGAGTTTGAA 
AGAAATGAAG ATTTCCAGCT TGGGCAGAGT GTAGACTTTG CTACAGCCAA GCCAACAACA 
CCGGCTCAAA CTCCTCAAAC CTCATCAGCA CCCAGCCATC GGGTCCCCAA CAGCAGCCTG 
GCAGGCTTCT ACCCTGTCAC AACAGGAGAT GGGGCAAGGG CTAGTCCCCA GTACCACCCT 
GCTGCAATGC TGCAGCATTC CACAGGGGGC GCTGTGTACA ATGGTGGGAA CTACAGTGGT 
GTGGACGGTG GGATGGCAAG CATTGGTGCT CAGAGTCAAG TGAAAGGATA TCCAGTTCAG 
TCTAATTTGA ATGGCCCAAC GGGACAGCTT TTGGGGACAT CCACTGCAAG TAACTACCAA 
ACGCAAGCAG GACTTGTGAA CTATCAGGCA GGCACCTTCC ATGGGTACCA AGGACAGCCA 
GTAATGAACA CACATCAAGG TCAAGTAGCT GTGAATAATC TTCAGCAACA GCAAGCTTTA 
AGCAGCTACC AAACAGTGAC AGGACAGAAC AGCTATTCCG TACAGAGAGG GGTGACTAGC 
TTTCCAACAC AGCCACCAAT GGGCACTTAC CAACAACATG TCGCAAACAG CAGTTTCCAG 
TCCCAAGCAG GGTTGAACAG CTATCCAGCA GCTGTAGGTC TTGCCTCTCA CCCTGTACCA 
GGTGTGTCAC AACATCAGAC AGTTGGACAA CCAAACCTCC AGCTGCTTGG ATCAATCCAA 
GACAGCCAGG TTCAGCCCTT GGTACCACCA CTTAACACCA AACCACCAAT GTCACTCCAC 
GGTGGGGCAT ACCCCAGTGT TGGACGAAGC TCATCCCCTC CAAAACCAGT GAACATAACA 
ACCGCTGAAG CTCAGGTCCC AAACAGTGCA GCAATGGTTA AATCTGGCCT GTATCCTATG 
TCAGCAGGAC TGACACAAGG TGTTAGTAGC ACTCCGAGTA GCACAATAGT TGGTGGTGGG 
CAAGGGGTGT ATCCATCTGT GCCAGCCTCA ACTGGTCTGT ACCCAGCAGC TGATCTAACA 
CAACCCAGCA TAGATCCAAA TTTTGCTGGA CTAGGAGGTA AAGGCAATGG GCTTTACCCA 
CAAACAGCAG CATTAAATCA GATGGCCAAT TTACCGACCA AGGGCAATGT TGGTATTACT 
GGTAGTGTGA CAGGTGGTGG GTTGTATCCT CAGACTACCG GCCTCCAGGC GACTTTAGGA 
ACTGATAATG TAGCTTCGAT ATTGCCTGGC ATTGGTGGAG GGGAGGCTAC TATGTATCCC 
AGCATCGGAC TGGCGAAGCC TGTAACAACA AATATCCAAG CTCAAGCAAC TGCTGATGAA 
TTAGTCAAAT ATATGATGAC TTCCCAGATC CTTAAAAAAG ACAGGGACAC ACAGCCGCCA 
CTGTCCACAG GTAATCCAAA TGTCAGCTTT GAATCTGCAC CAATCACTGT AAACCCAACG 
GGAGACAGAG GGCTGGAGGC AGCAGCTTCT TTAGATCTCA ATCTCGGAGG GAAACCAGCT 
GGAGAAACAC AAATGCCCAG CCTGCCAATG TCAAGAAGTG GTAAAACAGA TCAAGATAAC 
ATAGTCCAGG ACATGGGTGA AAAAAGGAAC GTTACAGCAC CAAGTACAGG TCTGAACATG 
CTGGAAGCCA ACGAGAAGGT AAACAAAGGT GATACTGTAC AAGGAGAGGC ACAGAAAAAT 
AGTCAGGAAA ACAACATTGT TGGGAACAAT GCTACTAATA AGGACATTAA AGTTGAGAAT 
AGTCAGTCAA CAGTCAATGT AAGAGATAGC TTTTCCAATG TACAACAAAC TGAAGTGTTA 
TCTGAAAATA AGGCAATGAT AGCACCTGAA CAAGTAGCAC ATAGCAGTCA AAGAACTGAA 
GAAAGTGGCG TTGTGTTGGG AGATGATAAA CCAAGTGAGC CTGTTCATTC CAATGATTTA 
ATGGATAGTG TTGGAAATAC AAATATGACT CAGAAATCCA GTATGGCATC TACACCATTG 
AAAGACAGTG ACAATGTGCC TGAGGGTGAT ATTGCTCGAG GGAATGGTTT AAAACAGAAG 
AAAGAAGTAG AGCATACTGC CACACAGGGT GTGAAAATTG CAGAAAATGC AGATGTCGTA 
AGCCAAGAAA AGGGTGGACC TCAAATGATA GGCTTCACTC CAGAGGAGGA GGTAGATGAA 
GCATTTGATG ATGCAGAAAT TGATGCATAC CTCGGGACGG AGGGAGTTGG CCTTGGAAAC 
TCTGGGAATG AGAGCATGAT GGAGGCAGGC AGAGAGGATG CTGGGATTCC TGAATCAGAT 
GCAGTATTGG AACAAGTCAT GGAAAGGTCT AGCCAGGAAG GGTCAGTTAC TGGTGTTTCA 
CAGCTACCAG TCACTATTTT CCTTCCAACT TCTGGTGCTG AGAGCAAAGA ACAAAAGTCA 
GACCAGGTTT TGAACAACAG TGAAGGGAAA ACTTACCCCA AAGATGGGGT CACAGCAGTT 
GGAGAACTCT CTGATGCTGA TCCTTTTTTC AGTGCACAGA CAAGTACCAG TCCAACTGAT 
ACAATAACAT CAATGGAAGA AGAAGAGGCT GTTACTGCAG CAGCATCAGT TGATAGTAAT 
ATACTGGATG GTAGGACTAT TGAACAAAAA GCTGGAAGCT CTGAGACTTT GATGAAGGGC 
GACTTGGAAG AAAGATTTCA GATTGAATCT AGAAAGCTTT CTGATATCAT TGGAAACAAT 
AGTAACATTG AGGACAGCAT TGCAGGAGCA CACGATGATG AGGATAGAAC TATCACATCA 
TTACAGGGGA CAGTCACACT TGGAGATAAT CCTGGTCACA TCCAGGAACA ACCCAGTCAC 
ACCCAGCAAC CACCTGGTTA CTCCCAACAA CCATCCGGTC ACACCCAGCA ACCACATGAT 
CATACCCAGC AACCACCTGG TTACACCCAG CAACCAAGTC AGCCAGAGGT AGACAGAAGT 
ATGGAGTCAG CCGTGGCAAA CATGGCGATC AGCGGACAAA TGGAACAGGC TCAACAAGAA 
CAACCATTCC CAGATTACAG CGAGACGTGG GACACCAGAC AGGCCCCGCA CCAGGACATG 
CCCCAGGACG GAGTCAGGAT GCCCCCACAC CCTCCCCTGG GAGTGGCCTC CCCGGAGGAT 
GTCGGGTTCC CACCCAATGT GGAGATGTTC AGGGATGACC TCAGTTCAGT TCTGCCATCA 
GATATCGGCT GGGAGGCCCC CGTATGGGTC CCCGACGAGG ATGCTCCAAA CTGCATGAAG 
TGTGAGTCCA AGTTCACGTT CACCAAGCGG CGGCACCACT GCAGGGCGTG CGGGAAGGTG 
CTGTGCTCGG CCTGCTGTAG TCAGAAGGCA CGGCTGGCGT ACATGGACAA CAAGTCAGCC 
CGCGTCTGCT CAACGTGCTA CAACATTCTA CAGAGAGCCC AGGCCAGAGG TCAGATTGCA 
GGCATCAGCC AGGTGACCCC ATCTTCAGGT CAGGCTTCCC CCAACCCTAA CAACCCCATG 
GAGTACTGCT CCACTCTGCC ACCTATGCAG CAGGCACAGG AAGCACTGCA GAGGCCACCC 
CCCAGTGTAC TGGTCCCTGT GGGTGGAAGG CCCCAAGGTG TTGCCCAGAG AGAAGGACGG 
CACGTGAGAT TTTCTGACGG AAGCATCCCC GGTGGGGACG GGACCTCCGA CTCCTCTACC 
CCCTCCACCC CCTCCCACCC TATCACTGCC CCCCAGGGAG CTGCGGCAGT GAGACCCAGG 
GCTGCACCAA ACACATCTCC TCAACATAAG GGGTCCCCCA GTAAAACAGC CGAGACTAGG 
GGTAGACTCA GTCCATCCAA GAACAGGGGG CAACACTCAG AGAGGCAATC AGGAAGTCCT 
CGCCAAGGAG GAAGTCCGAG GAAAACAGGG AGCCAAAGGT CACCCCCAAG GTCACGTCAG 
TCATCAAGGA CAAAGAGTCT AATTCCTGAG GAGGAAGACA GACTGCCTCC AGTCATCTTG 
TCTATAGGAG ACAAAGGAGA CTACGAGGTT GATTCGGAGC CGGACCCAGA CAGAACCCTG 
TCAGAGATAA AGAGGGAGGA GACGGACCCT GTCATGTTCC TGGCTAACCC CAACCTGGTG 
GTGCTGGTCA AGGTCATCAA CTTGTCGTGC TGTGTGAACA GGCTGTGCTG GTGTTTCTCC 
ACACGAGGGA TGCACACGGT CAGTCAGGAC GAGGTGGTCA TCCTGCTGGA GGCCATGCCT 
GATGATGATG TCATCCCCAG GGACATCTTT GCCCACCTGC AAATGGTGTA TGAGGAGGCA 
GGCAAAGGTA ATACAGTGGG AGACCTGGGC CATACCATCT TCAACCAGCC GTTCTTGGGC 
AGCCGTGAGC ATGGAGGTTT CCTGTACATC CAGCCCACCT TCCAGTGCAC CCAGCGCCTC 
ATCCTGCCGG ACCCCCCCTA TGTGGTGGGG GTGCTGCTGC AGAAGTGGGA GACACCCTGG 
GCAAAGGTCT TCCCCATACG CCTCATGCTT AGGCTGGGGG CTGAGTTCAG ATATTACCCC 
TGTCCACTGT TCAGCGTGCG GTTCCGCAAG CCAGTGTACT GTGAGATTGG ACATACCATC 
ATGAACCTAC TGGCTGATTT CCGTAACTAC CAGTACATGT TGGGACAGGT GAGGGGTGTG 
GTCATACTGC TGGAAGACAA GAGAACTCTG GTCAGGATAC CCAGCAACAG ATATGATGAT 
ATGATGAAAG TGCTGAACAA CTGTAATGAG CACGTCCTCT CCTGGGGCTC AAACTTCAGT 
GAGGAGGCGG ACTCCCATCT AGTGTGTATA CAGAACGACG AGGGAGCGTA CCAGACCCAG 
GCTATCAGCA TCCAGAACCG CCAACGGAAG GTCACCGGAG CCAGCTTTAT CGTGTTTAAC 
GGCTCGCTGA AGACAACGGC TGGGCTGAGG GGAAAGTCTA GCATTGTGGA GGATGGAGTG 
ATGGTACAGA TCCTACCTGA GACGATGTCG GAGCTTCGCC AGGCACTGCG GAACATGAAG 
GACTACACCA TCGGCTGTGG GAGCACGGCC TCTGAGGAAC CCGACGAGAC GGTGGAGGTC 
CGCTGGGTAG AGGACGACCG CAAAATGAAC ATTGGAGTCA CAAGTCCAAT TGATGGCCAG 
TCCATGGAGG GCTTGACCAA TATCCATGTG CACAGTGGCA TGGACTTTGC TGGGGAACAG 
AGGACCATTC GCTGGACCGA AGTCTTCTTC CTGCAAACTG AGGACTCTAT GCCCCAGTGT 
GAGCCCATGG AACTTAGCAG GCTGGCGGAG TCCATCGCCA CGGCAACCTG CGTGGCGCTG 
GTGCAGCACC TGGACTCCCT GAGGGAGGCT GGGATGAACA AACTGGGCCT GAGGGTCAAC 
ATAGACAGGG ACGAGGTTGG GTACGTAGCA GGTTCCAAGG GTCAGTTTCT GCCACCCCTG 
TACATGACCG ACCTTGACAA TGAACTCATC CCCGTCATCC ACAACGCAGC AGCGTCCAAT 
CAGGACACAC CCATCAGATT GGAGCTCATC TTCCATATTC TCCACAACGT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_91658 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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