BRAFLDRAFT_79985 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAFLDRAFT_79985
Entrez Gene ID	7216898
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Branchiostoma floridae(Florida lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002612316.1	XP_002612362.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Putnam NH, Butts T, Ferrier DE, Furlong RF, Hellsten U, Kawashima T, Robinson-Rechavi M, Shoguchi E, Terry A, Yu JK, Benito-Gutiérrez EL, Dubchak I, Garcia-Fernàndez J, Gibson-Brown JJ, Grigoriev IV, Horton AC, de Jong PJ, Jurka J, Kapitonov VV, Kohara Y, Kuroki Y, Lindquist E, Lucas S, Osoegawa K, Pennacchio LA, Salamov AA, Satou Y, Sauka-Spengler T, Schmutz J, Shin-I T, Toyoda A, Bronner-Fraser M, Fujiyama A, Holland LZ, Holland PW, Satoh N, Rokhsar DS
Nature453(7198)1064-71(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BRAFLDRAFT_79985 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAFLDRAFT_79985 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBb05158	XM_002612316.1 Latest version!	Branchiostoma floridae hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAFLDRAFT_79985 ( XM_002612316.1 ) cDNA ORF clone, Branchiostoma floridae(Florida lancelet) -> XP_002612362.1 Branchiostoma floridae hypothetical protein, mRNA.

Price & Availability↑

CloneID	OBb05158
Clone ID Related Accession (Same CDS sequence)	XM_002612316.1
Accession Version	XM_002612316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1254844800000
Organism	Branchiostoma floridae(Florida lancelet)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_003101557). COMPLETENESS: incomplete on both ends.

ATGGGGCGTT TGGAACCCCT CATTGTGATT GTGGTCTGTA CCGCCGTAGT TGTCGTGTGC 
GGACAACGGT GGCCAGGCCA GGTGACGACT CCGACTCCGG ATGGGTCGCA TGGGCCCTAT 
CCGTCTCCGG ATGGGTCGCA TGGTCCCTAT CCGTATCCTG ACGGCAGCGA ACAGCAGCCT 
AACGCCACCC ACAACTGCCG GGACCACCCA GAAATCTGCC GCTACCGACA GGAACTCAAG 
ACTTGGCAGG ACGGACAACA TGACAACGGG ACAGGGATCT GCCAACCGCT CCAATCCGTC 
TACGGACTTT CACCAATGCG ATTTTGTGCC GGACTTGGCG GCTGTGTTGT TGGCGACGAC 
TGCCGACGGT GGAGTGGTCT TCAATGTCTG CCAGGCACCG TGTTTTGTCC CGCGACCAAC 
CTCTGCTTAC CGACCAACAT GACTAGCTAC TGCATCAACG GCACCACGAT GTGCTCACAG 
CGTGAGAATG AGGAGTTTGC ACGCAAAGCT GTAAGTCTTG CACAAGAGTA CAGCTCACAC 
CCGCCCGACT GCCTTGGTTG TGGGTCGTCA TACTGTCGGC GGATAGTTGG TTATCGACAC 
TGTCATCAAT TGGATCAGAT TGCCTTTTGC CCGACATCGC GGCGGTGCAT TCTGGAAAAC 
GAAGCGTGTG AAGGCTTCAT CTCATTGGGA GCCCCTCCAG AACTATCGAC GGAAGGACCA 
CGGAGGGAAG AGCCAGTGAC GCACGAACCA AGGACGGAAG AACCACGGAG GGAAGAGACA 
GGGACGGACG AACCAAGGAC GGAAGAACCA CGGAGGGAAA GACCAGCGAC GGAAGAACCA 
CGGACGGAAG AACCACGGAG GGATGAACCA GGGACGGAAG AACCAGGGAC GGAAGACTCG 
TGGACGGAAG AACCAGGGAC GGAAGACTCG TGGACGGAAG AACCAGGGAC CGAAGACTCG 
TGGGCGGAAG AACCAGGGAC CGAAGAACCA CGGAGGGAAG GACCAGGGAC GGAAGAACCA 
CGGAGGGAAG GACCAGGGGC GGAAGAAACA GGAACGGGAG AACCAGAACC ATCAATGCCA 
GACCCATCAA CACTAAAGCC ATCTACACCC AAAACATCGA CCCCAGAACC ATTGACAACA 
GAGCGGTCAA CACTAAAGCC ATCTACTCCT AAAGCATCGA CTCCAAAACA ATCGACACTA 
GAGTCGTCAA CGCTGATGCC ATCTACACTT AAACCCTCGA CTCAAGAACT GTCGACACCA 
AAACCGTCGA CAGAAGACCC ATATGGCCAC GATGCCAACC CCAACTGCAC AATGATATTC 
TGCGCCGAGT ACGGAAGGTG CGTGCCAAGG GATAGCAGTT GCCATGGCGA CGGGAAGGGA 
GTGACTTGTT TACCTGGTCA GGCGTTCAGT TTGGAGTCAG GAAGGTGTGT TTCGACAGAT 
AAGAAGAAGG GGGAAGTAAC ATGTGGTAAC CGCGAGGTCT TCAGCGCGGA GCTCGGGACG 
TGTGTGTCGG CGGCCGTTTT GGACAAGACG CAGGCAGTCA CCTGCAGGCC TGGCGAGGTC 
TTCAATCTGG AAGAAGAAAG GTGTGTCACG GTGACCCCCC GAGCTCCGTA CAGCACGCAG 
GGAGGATCAT GCCAACCAGG GCAGAGTTTC TGCTTGCAGA CTGGCCGATG TGTCCCTGAC 
ACGTGTACAA CCACCACCAC TGTCGACAAG GAGAGAGCTG TTACCTGTAG TGGGAATGAG 
GTATTCTCCA TGCAGGCAAA CCGATGTGTC CAGGTCATGC CCACATCCAC TACCACCAAC 
GACGACACAG TTACCTGCAG TTTTCAACTT GCAGACAGGG CAATGCACAC GTGTCCCAAC 
CGCTGCCAGT TTTCAACTTG CAGACAGGGC AATGCACACG TGTCCCAACC GCTGCCAGTC 
TTTTGCTTGT CTGCTGGGCG CTGCATGCCT GTTAGTGGTG AATGTGGTGG TGTAGACGAA 
CATCGTCCGT CCGCTGAACC CAACTCTCCC AGCGGTCGAG ACGACAATGC CAAACCACCC 
ATATGTGGAA GGAAGGAGAC GTTATGTTTG TCCACCGATT CCTGTGTGAG GAATTGTTCT 
GAGAGCTGGG ATGACCCCAG GAAACTGCAC CTAAATGAGC AACAGGCAAA TAGCATGAGT 
CAGACAGAAG GAGGAAGACC AGGAAACAGG GGCCCTGAGA AAAATGGCCC AGACGCCAAA 
CCAGAGGGCG GAAAAACGGA AAAGGAAACC GAATGTGGCC GTCGTGAGGT TCAGGTCCAT 
GCGCCTGATG ATGGTGACTG CACATCTTTG TGTGCTGAGG AAGATGAGGA TGGGCCATTT 
GGAATCATCA AGAAGGAAGG TGACGACATG TTCAAGCTCC ATTTGTGCTA CACCAACACG 
TCTGTCCAAC AGCCATCTCC AGAGGATGTT GCCGGGGACA GGGGTGATCA GGGCCTTCGG 
CTGAAGGACC TGTTCAAAGT TTGCGACCCA ACAGTCTTTA AAGCACGGAA AGAGTTTGTG 
GTGCCTGCAG TGCCATACGA CCTGTCGGGA TGGGACGGGT ACACGGTGTC CGATGTGATG 
CGGCACTTTG TGGATGATGA GGAGCAGGAT GCCGTGGCTA TAAATGCTCC CTGGAGGGAC 
CTACGGATCA GTGGTGCCAG GCTGACTTTG AATCAGTACA TCAACTTCGA GCCATCATCC 
ACCACACGTG GCAGGATGGG GATTGACATC AGCGTACAGA GTGGAGGTCA GCCTGGGGAA 
CAGCATCGGT TCACATTTGA GGTAATTGAT AACGGAGTCA ACGACCCCCC AGTCTTTAAA 
GCGCGGAAAG AGTTTGTGGT GCCTGCAGTG CCATACGACC TGTCGGGATG GGACGGGTAC 
ACGGTGTCCG ATGTGATGCG GCACTTTGTG GATGATGAGG AGCAGGATAA CGTTGGTGCA 
GCCGTGGTAC TCAACGAACT TCGTTTGAGC GACATAGGAG TTTGGAGTGT GTCGCTCTCA 
GGGGACCCTG CGAGGTTTGA GCAGCTCAGA CCACCATCTC GTAACACCCG TGAAGTGACT 
CAGGGAGTGG AGCAACTTAA ATTGTTCGGT CCTGGTGCCA GGTTGAAATA TGAGCCGATT 
GAGCGTGACA GTCATTGGAG TTTCATTGAG GCAAGAAGGA AGACTGGACT GACCATCCGA 
GCCTGGGATG GTACAGCTTT TCAGGGAGGA CAAGTCATCA GTTATGATCC AACATGTCCA 
TTCTCTGAGG ACTGTGGAGG GTCAAACTCC ATCTCCAAGG AAAAAGTCAC AGTGGTCATA 
GATCGTGAAG GGTGTGACGG CAGGAGTGGG TCTGGGGTGA CCACAGACAG CTGTGGCGTG 
TGTAATGGAG ATGGGACAAG TTGCACTGAC GTCGACTGTA ATGGCGACAA CGGTGGAAGT 
GCGGTGAAGA ACAATTGCAG TCACTGCGTA GGGGGTAATA CAGGGAGGGC CAGCTCATAT 
GGTTTTGACT GTGCCAATAA GTGTGGCCTG TATGAAGTTC ACGAACTCTC TGGCAAGTGC 
ATTCCCAAGT GTGACCATTG TGTCGGTGGA AGTACTGGGA AAGATGTGAA CTTCGGAAAG 
AACGAGTGCG GTCATTGTGA CGACACGCGG GACTGCATCG ATTGTGAGGG TGTTGTGGGT 
GGAGGGAAAA AAGAGGATGA TTGTGGAGAA TGCCTTGATC CATTGGACCC CAACTTTAAC 
AAAGGATGCA TCGCGCTGTC CAGTGTGCGC CCAAGGATCC TGAAGGCTGG TGCAAAGACG 
ACTCTGACCG TGTCTGGAGC AGGGTTGCAA CAAATTGTTA ACGCGGAGTG CAAGATAACA 
TCAGTCAGCA GGGGAACACA GGGGGAGTTG GACGCTGTTC GCCTCAAACG TAAGGAGTGG 
AGCAACCAGG CCTTTGACTT GACATTCAAC GCCCCCAGAG CTGCAGGTGA ATTTGACCTT 
AGCTGTGATC TGAGTGGACG TGACGGCACG CTGGTCACCA AGGCACTGGA CCGAGAGAAC 
CGTCTCATCA TCATTCCCAG CGAGGGGATA CAGATCACCA GCGTTCAGCC TGACAACACT 
GAGATAGGTG GGGAGGATCC AGTGCCTGTC ACTGTTACAG GCCAAGGGTT TGTAGACACA 
GGTGACGCAT GGTGCTATGC CGTACACAAG CGAGCAACCA TCCAAGTGCC TGCAGAAATC 
ACCAGTGCAA CACAGGCAAC ATGCAGTCTG CCACCTTTGG AGAAGTCGGT TCGCGTTAAG 
ATTGGAGTGT CCCTGGAAAA ACCTGGAGAT GGCAAAAGGA GAGACACCGA CGACTACAGT 
GGCGGGTGGT TCGAGTACAG AACAGCGGCC CCGCAACTTC AGTCTGCACA ATTCTCAGAC 
AACCTCGCCT TGGTGACTGT GGTGTTCGAC AAGGCCATAG AGGGACCGAG AGATTGTCAG 
GGACTGTTTG ACAGGGCATC AGCTGACCAA GTCACCCTCA AGACAGATGC AGTCACTGCA 
CGGAGGTCAG AGGCTGGGGC AGCGGCGTCA GGCTCTGTAG ATGTCCTGGG ACCAGAAACA 
ATGCTTGTTC CACGTGTTGA GCTGCTGGGT CCATCTGAGG TAGATGCCTG TACCTGTCTG 
AAGCTGGAGG CCCGGTCTTA CAACATTGGT GGCAGGGCTG TTACTCACAG CTGGACAGTA 
GAGGTCGATG ATGCGTCTCT GGAATTGACT GACCTAAATG CCCACCTCAG TGGCCTGACC 
AGCAACAGTC GCATGCGGCT GTGTAAAGAC CAGGGTCTTC TGGAATCAGG CAAAGAGTAC 
ACTTTCTGTG TGACTGTCAC AAATTTCCTG GGTGCGGAGA GCGAACAATC CTGTCACGTG 
GTCCAGACGC TGGCGGAGGT CCATCCCTTC ACGGCAAGGA TCGCGGGCAG CCGCCGCCGA 
CAGACTGCTG CGGACCGCCG GCTCCGACTT CAGGCAAACG TGATGACACC ACCAGACAGC 
TGTGAGCAGG ATGTGGACAG CGTTACCAAC AACTTGAACT TCAGATGGGA GGTGGTAGGA 
AGTACTGACT TCAACTTGGA TAGCTTTACT GGCACCGTTG CATCCATCAC AGGAGGGATG 
AAAGGAGGCA AGACCTACTC TGTGCGCTTC ATCGCCTACA GCGACACTCA CTCCTCTGAA 
GACACAGTGG AAATAGTAGT TCGCAGTAGG GACCTACGGC CTGCTATTGC TGGCAAGACC 
ATCACCATAG GTAGTGATCA GGACGTCACC TTGGATGGAT CCGGATCACA GGACCCAGAC 
AATGCGGATG GGGATATGTA CTTTCAGTAC ACATTTTCCC TGATCATACG CAAGGGGAAC 
CGGTCAGCCA GCACTTCAGT GAAGCTGTTT GTTTCGCCGA ACGAAGTGCC ACAGGTGGAG 
GTGGACCCAG TCCCCGAGAA GGTCAACCCA GACAGGAGGG TGGTCATCAA CTTCAGGCTG 
ACCACTACTT TGCCGGACAC CACAGTCACA CTGGAGTGTT TGGAGCAGGA AGGATACGGT 
TACGTCGATT TGGAGGAGGT TACTGCCAAT GGAGAGACGA GCAAGACCTA CCCAAAACCT 
GCCACCAGGC AGCCGGTGAA TGTGGTGATC CTGCCAGACA GTCTGGCTCC TAACACTCAG 
TACACCTTCC GGGCCACCGC GACCCACTCA GGTGGAGAGG CATATGCCGA TGTTGTCATT 
GTGACCAATG CTGCGCCAAG CCCGGGTGAT TTTCAGGTGG AGCCAATGGA AGGCACGGCT 
CTGTCAGACA CCTTCACCCT CTCAGCAGAG GGCTGGACCG ACGATGAAGA CAATGCATTG 
GAGTATCGTT TCAGCTACAT AGGACCAGAC GGAAGGTCCG TATTGCTGAG GGGACGGACC 
GAGGACAGCC AATACGAGGC CACGCTTCCA GCAGGAACGG GTGCAGATAA CGAGTTGACA 
CTTGTGGTGG ACGTGTTCGA TGGGAGGCGG GCATCCAGTA GGATGACCTT GGAGGTCACG 
GTCAACCCTC CTGCTGAAAT CACCGATGAC ACTGTGGACA ATGTGAGGGG TGAAGTCGTG 
ACAAGCCTCC TTACAGGTGA TGTGGACAAA GCCATGTCTA GTGTGATTTC TATGGTGAAA 
ACTGTGAAGG AAGTTGGTGG CAACCTCACT GAGGCACTGC AAGATGTTGT CAACGAACTG 
AAGGCTGCAC TTACCAACAA GATCACAGAA ACAATTCCTA ATGACGAAGA GGAAGTGGAC 
AATGCACTAA GAGGTCTTGC CGGGACATTT GATGAAGACA GCAACATGGG AGAGGAGACC 
CGAACAAAGG CTAAGGACTA CATCAGTGGT CTTATTGACG ACTTCTACGA GATGGTTGAC 
GAAGTATCAG AGAATAATAA TGCCAGTAAC AATGGCCAGG ACAAGAGGAG GAGGAAACGT 
AATGTCAACA CCAATGTCGT GACCACTCCT ATGACGACAA CCCAGGTTTC TGACATGCTT 
CTAGTCTTCG ATGCCATGTT GGCCTCCATT GACGAAAGCA GTCCGGATGC AGCCACAACC 
AAGCTTGAGT TCCGCTCCAC TGTCGAATTG GGCATGGTTG GGCTGTGTCG AGGACAGTCG 
TATGGCGGCT CGGCCTCTGT GGCAATGTCG GGTAACGCTG TGGTGAGGAC CGATCTGACG 
TTGTTTGACG ACATTGCTGA CCAGCAGCTG CTGGCGTCGT GTGATGGTTG CAGTGGTGAC 
GTCACGGGCA CTCCAAAGAT CCGGCTCGGT ACAGACATAG CTTGGGCCTA CGAACAGTGG 
CAGTGCATTC CAGACTCGTC GACTACCTGT TATGGTGCAT GCATTGGGTC AGCGCAGATC 
AAGTTTGACC CCCTGACTGA CCCGCTGAGT AGTGATGTCA TCAGGAGTGA CCTGGTGCTG 
GTTGACCTGG TCAGTCCCCT GTCCGACCTG CGGACCCGTC TGGGCGGGCT GCGCGACCCC 
CTGGTGCTGG ACATCCCTGC CACTGGTGCC ATGCCTGACG ACACCTACAC TCTGGAGTGT 
CGACGCTGGG CAGGGAATGA GTGGACCACT GCTGGCTGCA GCACCAACCT CGTGCCTCGG 
GATGTTGACG GAGTGCCGTA TGTCACCTGC AGCTGTACCT TCATGGGAGG CTACTACGCG 
CTCTTTGAGG GTGTGTATGT GGAACCGACT ACCCCTGCCC CAGTCACCCC TCCCCCCGAG 
GAGAACGAGA TCGAGTTCAC GTTTGTGCAG AACTGCAGCG TGGTGACCAG TGGGGACGAG 
GCCGGCTTCC TCAGCTTGTT CCACAGCTTC ATCGCCGGGA AGATGAGGGT CTCAACTTCC 
CGCGTCAAGA ACCTGGCAAT CTTAGATGTT GTCGGTGTGG GGTGCATCTG CACCTATGAC 
CTGCTGGAGG CCACCGGCAC TGAGGACACC ATCAACTTCT ACGCAGTGGA TACCATCGCT 
GAGATGATGT GGAAGGGTAT CCTGGGTGCC AGCTCCCCTG ACGGGACCCC GCTGAACATC 
TACAGGTCAT CCCTGCTCAA GGATGGATTC ATGGTCAACC CAGCAAGGGT AGCTCCTGTC 
CAGAAGCCTG CCACCGCTGG TGAGATCATT GCGGGCCTGG TGTGCGGACT GTTCCTGTTC 
CTCATCATGC TCGGCCTGGC CTGCTGCTTT GCCCGGGCGT TGGTCGGCAA GGCCAAGGTG 
GAGGCGTCGC CACCATCCTC TCCTCCAACC TCAGCCCGCC GTGAAGCATT CGTGGCAGGA 
GGGTCCTCCC CACCATCCGG GAAGATGGAG ACCTACCCGA GGAACCTGGA GAAATCCGGC 
TTCCATGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002612362.1
CDS	1..7812
Translation	MGRLEPLIVIVVCTAVVVVCGQRWPGQVTTPTPDGSHGPYPSPDGSHGPYPYPDGSEQQPNATHNCRDHPEICRYRQELKTWQDGQHDNGTGICQPLQSVYGLSPMRFCAGLGGCVVGDDCRRWSGLQCLPGTVFCPATNLCLPTNMTSYCINGTTMCSQRENEEFARKAVSLAQEYSSHPPDCLGCGSSYCRRIVGYRHCHQLDQIAFCPTSRRCILENEACEGFISLGAPPELSTEGPRREEPVTHEPRTEEPRREETGTDEPRTEEPRRERPATEEPRTEEPRRDEPGTEEPGTEDSWTEEPGTEDSWTEEPGTEDSWAEEPGTEEPRREGPGTEEPRREGPGAEETGTGEPEPSMPDPSTLKPSTPKTSTPEPLTTERSTLKPSTPKASTPKQSTLESSTLMPSTLKPSTQELSTPKPSTEDPYGHDANPNCTMIFCAEYGRCVPRDSSCHGDGKGVTCLPGQAFSLESGRCVSTDKKKGEVTCGNREVFSAELGTCVSAAVLDKTQAVTCRPGEVFNLEEERCVTVTPRAPYSTQGGSCQPGQSFCLQTGRCVPDTCTTTTTVDKERAVTCSGNEVFSMQANRCVQVMPTSTTTNDDTVTCSFQLADRAMHTCPNRCQFSTCRQGNAHVSQPLPVFCLSAGRCMPVSGECGGVDEHRPSAEPNSPSGRDDNAKPPICGRKETLCLSTDSCVRNCSESWDDPRKLHLNEQQANSMSQTEGGRPGNRGPEKNGPDAKPEGGKTEKETECGRREVQVHAPDDGDCTSLCAEEDEDGPFGIIKKEGDDMFKLHLCYTNTSVQQPSPEDVAGDRGDQGLRLKDLFKVCDPTVFKARKEFVVPAVPYDLSGWDGYTVSDVMRHFVDDEEQDAVAINAPWRDLRISGARLTLNQYINFEPSSTTRGRMGIDISVQSGGQPGEQHRFTFEVIDNGVNDPPVFKARKEFVVPAVPYDLSGWDGYTVSDVMRHFVDDEEQDNVGAAVVLNELRLSDIGVWSVSLSGDPARFEQLRPPSRNTREVTQGVEQLKLFGPGARLKYEPIERDSHWSFIEARRKTGLTIRAWDGTAFQGGQVISYDPTCPFSEDCGGSNSISKEKVTVVIDREGCDGRSGSGVTTDSCGVCNGDGTSCTDVDCNGDNGGSAVKNNCSHCVGGNTGRASSYGFDCANKCGLYEVHELSGKCIPKCDHCVGGSTGKDVNFGKNECGHCDDTRDCIDCEGVVGGGKKEDDCGECLDPLDPNFNKGCIALSSVRPRILKAGAKTTLTVSGAGLQQIVNAECKITSVSRGTQGELDAVRLKRKEWSNQAFDLTFNAPRAAGEFDLSCDLSGRDGTLVTKALDRENRLIIIPSEGIQITSVQPDNTEIGGEDPVPVTVTGQGFVDTGDAWCYAVHKRATIQVPAEITSATQATCSLPPLEKSVRVKIGVSLEKPGDGKRRDTDDYSGGWFEYRTAAPQLQSAQFSDNLALVTVVFDKAIEGPRDCQGLFDRASADQVTLKTDAVTARRSEAGAAASGSVDVLGPETMLVPRVELLGPSEVDACTCLKLEARSYNIGGRAVTHSWTVEVDDASLELTDLNAHLSGLTSNSRMRLCKDQGLLESGKEYTFCVTVTNFLGAESEQSCHVVQTLAEVHPFTARIAGSRRRQTAADRRLRLQANVMTPPDSCEQDVDSVTNNLNFRWEVVGSTDFNLDSFTGTVASITGGMKGGKTYSVRFIAYSDTHSSEDTVEIVVRSRDLRPAIAGKTITIGSDQDVTLDGSGSQDPDNADGDMYFQYTFSLIIRKGNRSASTSVKLFVSPNEVPQVEVDPVPEKVNPDRRVVINFRLTTTLPDTTVTLECLEQEGYGYVDLEEVTANGETSKTYPKPATRQPVNVVILPDSLAPNTQYTFRATATHSGGEAYADVVIVTNAAPSPGDFQVEPMEGTALSDTFTLSAEGWTDDEDNALEYRFSYIGPDGRSVLLRGRTEDSQYEATLPAGTGADNELTLVVDVFDGRRASSRMTLEVTVNPPAEITDDTVDNVRGEVVTSLLTGDVDKAMSSVISMVKTVKEVGGNLTEALQDVVNELKAALTNKITETIPNDEEEVDNALRGLAGTFDEDSNMGEETRTKAKDYISGLIDDFYEMVDEVSENNNASNNGQDKRRRKRNVNTNVVTTPMTTTQVSDMLLVFDAMLASIDESSPDAATTKLEFRSTVELGMVGLCRGQSYGGSASVAMSGNAVVRTDLTLFDDIADQQLLASCDGCSGDVTGTPKIRLGTDIAWAYEQWQCIPDSSTTCYGACIGSAQIKFDPLTDPLSSDVIRSDLVLVDLVSPLSDLRTRLGGLRDPLVLDIPATGAMPDDTYTLECRRWAGNEWTTAGCSTNLVPRDVDGVPYVTCSCTFMGGYYALFEGVYVEPTTPAPVTPPPEENEIEFTFVQNCSVVTSGDEAGFLSLFHSFIAGKMRVSTSRVKNLAILDVVGVGCICTYDLLEATGTEDTINFYAVDTIAEMMWKGILGASSPDGTPLNIYRSSLLKDGFMVNPARVAPVQKPATAGEIIAGLVCGLFLFLIMLGLACCFARALVGKAKVEASPPSSPPTSARREAFVAGGSSPPSGKMETYPRNLEKSGFHV

Target ORF information:

RefSeq Version	XM_002612316.1
Organism	Branchiostoma floridae(Florida lancelet)
Definition	Branchiostoma floridae hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002612316.1

ORF Insert Sequence:

ATGGGGCGTT TGGAACCCCT CATTGTGATT GTGGTCTGTA CCGCCGTAGT TGTCGTGTGC 
GGACAACGGT GGCCAGGCCA GGTGACGACT CCGACTCCGG ATGGGTCGCA TGGGCCCTAT 
CCGTCTCCGG ATGGGTCGCA TGGTCCCTAT CCGTATCCTG ACGGCAGCGA ACAGCAGCCT 
AACGCCACCC ACAACTGCCG GGACCACCCA GAAATCTGCC GCTACCGACA GGAACTCAAG 
ACTTGGCAGG ACGGACAACA TGACAACGGG ACAGGGATCT GCCAACCGCT CCAATCCGTC 
TACGGACTTT CACCAATGCG ATTTTGTGCC GGACTTGGCG GCTGTGTTGT TGGCGACGAC 
TGCCGACGGT GGAGTGGTCT TCAATGTCTG CCAGGCACCG TGTTTTGTCC CGCGACCAAC 
CTCTGCTTAC CGACCAACAT GACTAGCTAC TGCATCAACG GCACCACGAT GTGCTCACAG 
CGTGAGAATG AGGAGTTTGC ACGCAAAGCT GTAAGTCTTG CACAAGAGTA CAGCTCACAC 
CCGCCCGACT GCCTTGGTTG TGGGTCGTCA TACTGTCGGC GGATAGTTGG TTATCGACAC 
TGTCATCAAT TGGATCAGAT TGCCTTTTGC CCGACATCGC GGCGGTGCAT TCTGGAAAAC 
GAAGCGTGTG AAGGCTTCAT CTCATTGGGA GCCCCTCCAG AACTATCGAC GGAAGGACCA 
CGGAGGGAAG AGCCAGTGAC GCACGAACCA AGGACGGAAG AACCACGGAG GGAAGAGACA 
GGGACGGACG AACCAAGGAC GGAAGAACCA CGGAGGGAAA GACCAGCGAC GGAAGAACCA 
CGGACGGAAG AACCACGGAG GGATGAACCA GGGACGGAAG AACCAGGGAC GGAAGACTCG 
TGGACGGAAG AACCAGGGAC GGAAGACTCG TGGACGGAAG AACCAGGGAC CGAAGACTCG 
TGGGCGGAAG AACCAGGGAC CGAAGAACCA CGGAGGGAAG GACCAGGGAC GGAAGAACCA 
CGGAGGGAAG GACCAGGGGC GGAAGAAACA GGAACGGGAG AACCAGAACC ATCAATGCCA 
GACCCATCAA CACTAAAGCC ATCTACACCC AAAACATCGA CCCCAGAACC ATTGACAACA 
GAGCGGTCAA CACTAAAGCC ATCTACTCCT AAAGCATCGA CTCCAAAACA ATCGACACTA 
GAGTCGTCAA CGCTGATGCC ATCTACACTT AAACCCTCGA CTCAAGAACT GTCGACACCA 
AAACCGTCGA CAGAAGACCC ATATGGCCAC GATGCCAACC CCAACTGCAC AATGATATTC 
TGCGCCGAGT ACGGAAGGTG CGTGCCAAGG GATAGCAGTT GCCATGGCGA CGGGAAGGGA 
GTGACTTGTT TACCTGGTCA GGCGTTCAGT TTGGAGTCAG GAAGGTGTGT TTCGACAGAT 
AAGAAGAAGG GGGAAGTAAC ATGTGGTAAC CGCGAGGTCT TCAGCGCGGA GCTCGGGACG 
TGTGTGTCGG CGGCCGTTTT GGACAAGACG CAGGCAGTCA CCTGCAGGCC TGGCGAGGTC 
TTCAATCTGG AAGAAGAAAG GTGTGTCACG GTGACCCCCC GAGCTCCGTA CAGCACGCAG 
GGAGGATCAT GCCAACCAGG GCAGAGTTTC TGCTTGCAGA CTGGCCGATG TGTCCCTGAC 
ACGTGTACAA CCACCACCAC TGTCGACAAG GAGAGAGCTG TTACCTGTAG TGGGAATGAG 
GTATTCTCCA TGCAGGCAAA CCGATGTGTC CAGGTCATGC CCACATCCAC TACCACCAAC 
GACGACACAG TTACCTGCAG TTTTCAACTT GCAGACAGGG CAATGCACAC GTGTCCCAAC 
CGCTGCCAGT TTTCAACTTG CAGACAGGGC AATGCACACG TGTCCCAACC GCTGCCAGTC 
TTTTGCTTGT CTGCTGGGCG CTGCATGCCT GTTAGTGGTG AATGTGGTGG TGTAGACGAA 
CATCGTCCGT CCGCTGAACC CAACTCTCCC AGCGGTCGAG ACGACAATGC CAAACCACCC 
ATATGTGGAA GGAAGGAGAC GTTATGTTTG TCCACCGATT CCTGTGTGAG GAATTGTTCT 
GAGAGCTGGG ATGACCCCAG GAAACTGCAC CTAAATGAGC AACAGGCAAA TAGCATGAGT 
CAGACAGAAG GAGGAAGACC AGGAAACAGG GGCCCTGAGA AAAATGGCCC AGACGCCAAA 
CCAGAGGGCG GAAAAACGGA AAAGGAAACC GAATGTGGCC GTCGTGAGGT TCAGGTCCAT 
GCGCCTGATG ATGGTGACTG CACATCTTTG TGTGCTGAGG AAGATGAGGA TGGGCCATTT 
GGAATCATCA AGAAGGAAGG TGACGACATG TTCAAGCTCC ATTTGTGCTA CACCAACACG 
TCTGTCCAAC AGCCATCTCC AGAGGATGTT GCCGGGGACA GGGGTGATCA GGGCCTTCGG 
CTGAAGGACC TGTTCAAAGT TTGCGACCCA ACAGTCTTTA AAGCACGGAA AGAGTTTGTG 
GTGCCTGCAG TGCCATACGA CCTGTCGGGA TGGGACGGGT ACACGGTGTC CGATGTGATG 
CGGCACTTTG TGGATGATGA GGAGCAGGAT GCCGTGGCTA TAAATGCTCC CTGGAGGGAC 
CTACGGATCA GTGGTGCCAG GCTGACTTTG AATCAGTACA TCAACTTCGA GCCATCATCC 
ACCACACGTG GCAGGATGGG GATTGACATC AGCGTACAGA GTGGAGGTCA GCCTGGGGAA 
CAGCATCGGT TCACATTTGA GGTAATTGAT AACGGAGTCA ACGACCCCCC AGTCTTTAAA 
GCGCGGAAAG AGTTTGTGGT GCCTGCAGTG CCATACGACC TGTCGGGATG GGACGGGTAC 
ACGGTGTCCG ATGTGATGCG GCACTTTGTG GATGATGAGG AGCAGGATAA CGTTGGTGCA 
GCCGTGGTAC TCAACGAACT TCGTTTGAGC GACATAGGAG TTTGGAGTGT GTCGCTCTCA 
GGGGACCCTG CGAGGTTTGA GCAGCTCAGA CCACCATCTC GTAACACCCG TGAAGTGACT 
CAGGGAGTGG AGCAACTTAA ATTGTTCGGT CCTGGTGCCA GGTTGAAATA TGAGCCGATT 
GAGCGTGACA GTCATTGGAG TTTCATTGAG GCAAGAAGGA AGACTGGACT GACCATCCGA 
GCCTGGGATG GTACAGCTTT TCAGGGAGGA CAAGTCATCA GTTATGATCC AACATGTCCA 
TTCTCTGAGG ACTGTGGAGG GTCAAACTCC ATCTCCAAGG AAAAAGTCAC AGTGGTCATA 
GATCGTGAAG GGTGTGACGG CAGGAGTGGG TCTGGGGTGA CCACAGACAG CTGTGGCGTG 
TGTAATGGAG ATGGGACAAG TTGCACTGAC GTCGACTGTA ATGGCGACAA CGGTGGAAGT 
GCGGTGAAGA ACAATTGCAG TCACTGCGTA GGGGGTAATA CAGGGAGGGC CAGCTCATAT 
GGTTTTGACT GTGCCAATAA GTGTGGCCTG TATGAAGTTC ACGAACTCTC TGGCAAGTGC 
ATTCCCAAGT GTGACCATTG TGTCGGTGGA AGTACTGGGA AAGATGTGAA CTTCGGAAAG 
AACGAGTGCG GTCATTGTGA CGACACGCGG GACTGCATCG ATTGTGAGGG TGTTGTGGGT 
GGAGGGAAAA AAGAGGATGA TTGTGGAGAA TGCCTTGATC CATTGGACCC CAACTTTAAC 
AAAGGATGCA TCGCGCTGTC CAGTGTGCGC CCAAGGATCC TGAAGGCTGG TGCAAAGACG 
ACTCTGACCG TGTCTGGAGC AGGGTTGCAA CAAATTGTTA ACGCGGAGTG CAAGATAACA 
TCAGTCAGCA GGGGAACACA GGGGGAGTTG GACGCTGTTC GCCTCAAACG TAAGGAGTGG 
AGCAACCAGG CCTTTGACTT GACATTCAAC GCCCCCAGAG CTGCAGGTGA ATTTGACCTT 
AGCTGTGATC TGAGTGGACG TGACGGCACG CTGGTCACCA AGGCACTGGA CCGAGAGAAC 
CGTCTCATCA TCATTCCCAG CGAGGGGATA CAGATCACCA GCGTTCAGCC TGACAACACT 
GAGATAGGTG GGGAGGATCC AGTGCCTGTC ACTGTTACAG GCCAAGGGTT TGTAGACACA 
GGTGACGCAT GGTGCTATGC CGTACACAAG CGAGCAACCA TCCAAGTGCC TGCAGAAATC 
ACCAGTGCAA CACAGGCAAC ATGCAGTCTG CCACCTTTGG AGAAGTCGGT TCGCGTTAAG 
ATTGGAGTGT CCCTGGAAAA ACCTGGAGAT GGCAAAAGGA GAGACACCGA CGACTACAGT 
GGCGGGTGGT TCGAGTACAG AACAGCGGCC CCGCAACTTC AGTCTGCACA ATTCTCAGAC 
AACCTCGCCT TGGTGACTGT GGTGTTCGAC AAGGCCATAG AGGGACCGAG AGATTGTCAG 
GGACTGTTTG ACAGGGCATC AGCTGACCAA GTCACCCTCA AGACAGATGC AGTCACTGCA 
CGGAGGTCAG AGGCTGGGGC AGCGGCGTCA GGCTCTGTAG ATGTCCTGGG ACCAGAAACA 
ATGCTTGTTC CACGTGTTGA GCTGCTGGGT CCATCTGAGG TAGATGCCTG TACCTGTCTG 
AAGCTGGAGG CCCGGTCTTA CAACATTGGT GGCAGGGCTG TTACTCACAG CTGGACAGTA 
GAGGTCGATG ATGCGTCTCT GGAATTGACT GACCTAAATG CCCACCTCAG TGGCCTGACC 
AGCAACAGTC GCATGCGGCT GTGTAAAGAC CAGGGTCTTC TGGAATCAGG CAAAGAGTAC 
ACTTTCTGTG TGACTGTCAC AAATTTCCTG GGTGCGGAGA GCGAACAATC CTGTCACGTG 
GTCCAGACGC TGGCGGAGGT CCATCCCTTC ACGGCAAGGA TCGCGGGCAG CCGCCGCCGA 
CAGACTGCTG CGGACCGCCG GCTCCGACTT CAGGCAAACG TGATGACACC ACCAGACAGC 
TGTGAGCAGG ATGTGGACAG CGTTACCAAC AACTTGAACT TCAGATGGGA GGTGGTAGGA 
AGTACTGACT TCAACTTGGA TAGCTTTACT GGCACCGTTG CATCCATCAC AGGAGGGATG 
AAAGGAGGCA AGACCTACTC TGTGCGCTTC ATCGCCTACA GCGACACTCA CTCCTCTGAA 
GACACAGTGG AAATAGTAGT TCGCAGTAGG GACCTACGGC CTGCTATTGC TGGCAAGACC 
ATCACCATAG GTAGTGATCA GGACGTCACC TTGGATGGAT CCGGATCACA GGACCCAGAC 
AATGCGGATG GGGATATGTA CTTTCAGTAC ACATTTTCCC TGATCATACG CAAGGGGAAC 
CGGTCAGCCA GCACTTCAGT GAAGCTGTTT GTTTCGCCGA ACGAAGTGCC ACAGGTGGAG 
GTGGACCCAG TCCCCGAGAA GGTCAACCCA GACAGGAGGG TGGTCATCAA CTTCAGGCTG 
ACCACTACTT TGCCGGACAC CACAGTCACA CTGGAGTGTT TGGAGCAGGA AGGATACGGT 
TACGTCGATT TGGAGGAGGT TACTGCCAAT GGAGAGACGA GCAAGACCTA CCCAAAACCT 
GCCACCAGGC AGCCGGTGAA TGTGGTGATC CTGCCAGACA GTCTGGCTCC TAACACTCAG 
TACACCTTCC GGGCCACCGC GACCCACTCA GGTGGAGAGG CATATGCCGA TGTTGTCATT 
GTGACCAATG CTGCGCCAAG CCCGGGTGAT TTTCAGGTGG AGCCAATGGA AGGCACGGCT 
CTGTCAGACA CCTTCACCCT CTCAGCAGAG GGCTGGACCG ACGATGAAGA CAATGCATTG 
GAGTATCGTT TCAGCTACAT AGGACCAGAC GGAAGGTCCG TATTGCTGAG GGGACGGACC 
GAGGACAGCC AATACGAGGC CACGCTTCCA GCAGGAACGG GTGCAGATAA CGAGTTGACA 
CTTGTGGTGG ACGTGTTCGA TGGGAGGCGG GCATCCAGTA GGATGACCTT GGAGGTCACG 
GTCAACCCTC CTGCTGAAAT CACCGATGAC ACTGTGGACA ATGTGAGGGG TGAAGTCGTG 
ACAAGCCTCC TTACAGGTGA TGTGGACAAA GCCATGTCTA GTGTGATTTC TATGGTGAAA 
ACTGTGAAGG AAGTTGGTGG CAACCTCACT GAGGCACTGC AAGATGTTGT CAACGAACTG 
AAGGCTGCAC TTACCAACAA GATCACAGAA ACAATTCCTA ATGACGAAGA GGAAGTGGAC 
AATGCACTAA GAGGTCTTGC CGGGACATTT GATGAAGACA GCAACATGGG AGAGGAGACC 
CGAACAAAGG CTAAGGACTA CATCAGTGGT CTTATTGACG ACTTCTACGA GATGGTTGAC 
GAAGTATCAG AGAATAATAA TGCCAGTAAC AATGGCCAGG ACAAGAGGAG GAGGAAACGT 
AATGTCAACA CCAATGTCGT GACCACTCCT ATGACGACAA CCCAGGTTTC TGACATGCTT 
CTAGTCTTCG ATGCCATGTT GGCCTCCATT GACGAAAGCA GTCCGGATGC AGCCACAACC 
AAGCTTGAGT TCCGCTCCAC TGTCGAATTG GGCATGGTTG GGCTGTGTCG AGGACAGTCG 
TATGGCGGCT CGGCCTCTGT GGCAATGTCG GGTAACGCTG TGGTGAGGAC CGATCTGACG 
TTGTTTGACG ACATTGCTGA CCAGCAGCTG CTGGCGTCGT GTGATGGTTG CAGTGGTGAC 
GTCACGGGCA CTCCAAAGAT CCGGCTCGGT ACAGACATAG CTTGGGCCTA CGAACAGTGG 
CAGTGCATTC CAGACTCGTC GACTACCTGT TATGGTGCAT GCATTGGGTC AGCGCAGATC 
AAGTTTGACC CCCTGACTGA CCCGCTGAGT AGTGATGTCA TCAGGAGTGA CCTGGTGCTG 
GTTGACCTGG TCAGTCCCCT GTCCGACCTG CGGACCCGTC TGGGCGGGCT GCGCGACCCC 
CTGGTGCTGG ACATCCCTGC CACTGGTGCC ATGCCTGACG ACACCTACAC TCTGGAGTGT 
CGACGCTGGG CAGGGAATGA GTGGACCACT GCTGGCTGCA GCACCAACCT CGTGCCTCGG 
GATGTTGACG GAGTGCCGTA TGTCACCTGC AGCTGTACCT TCATGGGAGG CTACTACGCG 
CTCTTTGAGG GTGTGTATGT GGAACCGACT ACCCCTGCCC CAGTCACCCC TCCCCCCGAG 
GAGAACGAGA TCGAGTTCAC GTTTGTGCAG AACTGCAGCG TGGTGACCAG TGGGGACGAG 
GCCGGCTTCC TCAGCTTGTT CCACAGCTTC ATCGCCGGGA AGATGAGGGT CTCAACTTCC 
CGCGTCAAGA ACCTGGCAAT CTTAGATGTT GTCGGTGTGG GGTGCATCTG CACCTATGAC 
CTGCTGGAGG CCACCGGCAC TGAGGACACC ATCAACTTCT ACGCAGTGGA TACCATCGCT 
GAGATGATGT GGAAGGGTAT CCTGGGTGCC AGCTCCCCTG ACGGGACCCC GCTGAACATC 
TACAGGTCAT CCCTGCTCAA GGATGGATTC ATGGTCAACC CAGCAAGGGT AGCTCCTGTC 
CAGAAGCCTG CCACCGCTGG TGAGATCATT GCGGGCCTGG TGTGCGGACT GTTCCTGTTC 
CTCATCATGC TCGGCCTGGC CTGCTGCTTT GCCCGGGCGT TGGTCGGCAA GGCCAAGGTG 
GAGGCGTCGC CACCATCCTC TCCTCCAACC TCAGCCCGCC GTGAAGCATT CGTGGCAGGA 
GGGTCCTCCC CACCATCCGG GAAGATGGAG ACCTACCCGA GGAACCTGGA GAAATCCGGC 
TTCCATGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The amphioxus genome and the evolution of the chordate karyotype.
Nature453(7198)1064-71(2008 Jun)
Putnam NH,Butts T,Ferrier DE,Furlong RF,Hellsten U,Kawashima T,Robinson-Rechavi M,Shoguchi E,Terry A,Yu JK,Benito-Gutiérrez EL,Dubchak I,Garcia-Fernàndez J,Gibson-Brown JJ,Grigoriev IV,Horton AC,de Jong PJ,Jurka J,Kapitonov VV,Kohara Y,Kuroki Y,Lindquist E,Lucas S,Osoegawa K,Pennacchio LA,Salamov AA,Satou Y,Sauka-Spengler T,Schmutz J,Shin-I T,Toyoda A,Bronner-Fraser M,Fujiyama A,Holland LZ,Holland PW,Satoh N,Rokhsar DS

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BRAFLDRAFT_79985 cDNA ORF clone, Branchiostoma floridae(Florida lancelet)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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