NEMF cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEMF
Entrez Gene ID	100931443
Full Name	nuclear export mediator factor
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012549075.1	XP_012404529.1	nuclear export mediator factor NEMF
XM_023503090.1	XP_023358858.1	nuclear export mediator factor NEMF
XM_031952816.1	XP_031808676.1	nuclear export mediator factor NEMF isoform X1
XM_031952817.1	XP_031808677.1	nuclear export mediator factor NEMF isoform X1
XM_031952818.1	XP_031808678.1	nuclear export mediator factor NEMF isoform X2

Pathways from BioSystems
Gene Ontology

The following NEMF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEMF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa117870	XM_012549075.1 Latest version!	Sarcophilus harrisii nuclear export mediator factor (NEMF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa149790	XM_023503090.1 Latest version!	Sarcophilus harrisii nuclear export mediator factor (NEMF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa225339	XM_031952817.1 Latest version!	Sarcophilus harrisii nuclear export mediator factor (NEMF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa225339	XM_031952816.1 Latest version!	Sarcophilus harrisii nuclear export mediator factor (NEMF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa225340	XM_031952818.1 Latest version!	Sarcophilus harrisii nuclear export mediator factor (NEMF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEMF ( XM_012549075.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012404529.1 Sarcophilus harrisii nuclear export mediator factor (NEMF), mRNA.

Price & Availability↑

CloneID	OSa117870
Clone ID Related Accession (Same CDS sequence)	XM_012549075.1
Accession Version	XM_012549075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	nuclear export mediator factor NEMF
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816564.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAAGACGC GTTTCAGCTC CGTAGATATT TGCGCTATCC TCTCCGAGTT CAATGCCAGA 
TTATTGGGAA TGAGAGTATA CAATATTTAT GATGTGGATA ATAAGACATA CCTTATACGC 
CTTCAAAAGC CAGACTTTAA AGTTACGCTT CTACTTGAAT CTGGTATACG AATTCACACG 
ACAGAGTTTG AATGGCCAAA GAATATGATG CCATCTAGCT TTGCCATGAA GTGCCGAAAG 
CATTTGAAGA GTCGAAGACT GGTCAGTGTA AAACAGCTTG GTGTGGATCG GATTGTAGAT 
TTTCAGTTTG GAAGTGATGA AGCTGCCTAT CATTTAATCA TTGAGCTTTA TGATAAAGGC 
AATATTGTTC TTACCAACTA TGAATACCTC ATTTTAAATA TCTTGAGGTT TAGAAGTGAT 
GAAGCAGATG ATGTGAAGTT TGCTGTTCGA GAAAAATATC CAATTGATCA TGCTAGGGTA 
ATGGAACCAT TACTTTCACT GGAAAGATTA ACTGAAATAA TATCCAGTGC ACCAAAAGGA 
GAACAATTAA GGAGGATTCT TAATCCATAT CTCCCCTACG GGGCCACTCT GATTGAACAC 
TGTCTTAGAG AAAATGGATT TTCCAGCTAT TTCAGAGTAG ATGAGAAATT TGAAACTGGA 
GATATTGAAA AAGTACTTGT TTGTCTGCAA AAAGCAGAAG ATCATATGAA AACAATGTCC 
AACTTCAGTG GAAAGGGGTA TATCATTCAG AAAAAAGAGA AAAAACCAAG CCTGGAGCCA 
GATAAGCAAT CTGAAGATAT ACTCACATAT GAAGAATTTC ATCCTTTCTT ATTTTCTCAA 
CATTCCAAAT GTCCATATAT AGAATTTGAA TCATTTGACA AGGCAGTGGA TGAATTTTAT 
TCTAAGCTAG AAGGCCAGAA GATAGACTTG AAAGCTTTAC AACAGGAGAA ACAAGCATTG 
AAGAAATTGG ATAATGTCCG TAAGGATCAT GAGCACAGAT TAGAAGCCCT TCACCAAGCT 
CAGGAAATTG ATAAAATCAA AGGAGAACTC ATAGAAATGA ATCTACAGAT AGTTGATAGA 
GCCATCCAAG TAGTTCGGAG TGCTTTAGCC AACCAGATAG ACTGGACAGA AATTGGACTA 
ATTGTAAAAG AGGCCCAAGC TCAAGGAGAT ATTGTTGCAA ATGCAATCAG AGAATTGAAG 
CTGCAAACCA ATCATGTCAC AATGCTGCTA AAAAATCCAT ATCTAATATC AGATGAGGAG 
GAGGAAGATG ATGAAATTAA TATTGAAAAA GAAGAAACAG AAGAACCAAA AGGGAAAAAA 
AAAAAGCAGA AGAACAAACA ACTTCAGAAA CTTCAGAAGA ATAAGCCATT ACTTGTTGAT 
GTTGATCTCA GTTTGTCAGC ATATGCCAAT GCCAAAAAAT ATTATGACCA CAAGAGACAT 
GCTGCCAGAA AAACACAAAA AACTGTTGAA GCTGCTGAGA AGGCATTCAG ATCTGCGGAA 
AAGAAAACAA AACAAACCCT CAAAGAAGTT CAGATGGTTA CCACTATTCA GAAAGCAAGG 
AAAGTGTATT GTATTTCTCT TGTAATTGAA TCTAATATTA TTTTTCAGGT CTCTAAAACA 
GCACCAACTG GAGAATATTT GACAACAGGA AGCTTCATGA TAAGAGGAAA GAAGAATTTT 
CTTCCTCCTT CATATCTTAT GATGGGGTTT AGTTTCCTAT TTAAGGTAGA TGAACCATGT 
GTTTGGAGAC ATCGAGGGGA ACGGAAAGTC AGAGGTCAAG ATGAAGATTT GGAGACAGTG 
GCTAGCAGCA CTAGTAAGCT AGTTTCAGAA GAAATGGAGC CATTAGATAA TGGTGATAGC 
AGCAGTGGAG AGGATCAAGA AGAAACTTCT GAAACTGTAG AAGAAAGGGA AGTAGTAAAT 
CAAATTGATG AAGAGGTCAT TAGCATTCAA AATGACAAAA ACAGGCCAAA AGAAGGATCA 
GCACAAGAAG AGAGCTCAGA TGATGATGCT GCGGATGATG CTGATGCTGA TGCTGATGAT 
GATGAGGAGG AGGAGGAGGA CGATGACGAC GACGATGATG ATCATGATCA TGATAACACC 
AATGACGATA GAAAATCTCA AAGAATGAAA TCAGATCAGG AAATAGCAAG TAAAAGGAAG 
GATGAATCTG AAATGTCATT AAATTATCCA GATACTACAA TTGATCTATC TCACCTTCAG 
TCCCAAAGAT CCTTCCAGAA AACAGTTACT AGAGAAGATG CTTCTGATGT GAATGACAGT 
AAATTACATG GCCGGAGGCA TTTGTCCGCC AAGGAACGAA GGGAAATGAA GAAAAAAAAG 
CAACCAAATG ATTCAACAGA TTTAGATATA TTGGAGGACA AGGGAAAAGA AAATACACTG 
AAGACTGAAG TTTTTCCAAA TACAAGCAAA ACTGTTTCGG GTCCACAACC AATGAAGAGA 
GGACAGAAGA GTAAAATTAA AAAAATGAAA GAAAAATATA AAGATCAAGA TGAGGAAGAT 
CGTGAACTTA TCATGAAGCT CCTTGGATCT GCAGGTTCAA GCAAAGAAGA AAAAGGAAAG 
AAAGGGAAGA AAGGAAAAAC GGGGAAAACA AAAGAAGAGG CAACAAAGAA ACAACCCCAG 
AAGTTCAGAA GTGAACTGAG AATTGGTGAC AGAATTAAGA AAGAAACTCC TCTTGAAGCT 
GTAATTCATG AATTACAAGA AATTACCATG GACGATCAGC CAGATGATAA GGAAGAACAA 
GATGTAGACC AACAAGGAAA TGAGGAGAAC CTGCTGGATT CTTTAACAGG ACAACCCCAC 
TCTGAAGATG TTTTGCTTTT TGCTATACCA GTATGTGCCC CTTATACCAC CATGACCAAC 
TATAAATACA AAGTGAAACT TACTCCTGGA GTCCAAAAAA AAGGAAAAGC GGCTAAAACT 
GCCTTGAATA CTTTCATGCA TTCAAAAGAA GCAACAGCAA GAGAAAAAGA TTTATTTCGC 
AGTGTAAAGG ACACTGATTT ATCAAGAAAT ATTCCTGGAA AAGTGAAAGT ATCTGCTCCC 
AATCTCCTTA ATGCAAAGAA GAAAAATAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012404529.1
CDS	89..3181
Translation	MKTRFSSVDICAILSEFNARLLGMRVYNIYDVDNKTYLIRLQKPDFKVTLLLESGIRIHTTEFEWPKNMMPSSFAMKCRKHLKSRRLVSVKQLGVDRIVDFQFGSDEAAYHLIIELYDKGNIVLTNYEYLILNILRFRSDEADDVKFAVREKYPIDHARVMEPLLSLERLTEIISSAPKGEQLRRILNPYLPYGATLIEHCLRENGFSSYFRVDEKFETGDIEKVLVCLQKAEDHMKTMSNFSGKGYIIQKKEKKPSLEPDKQSEDILTYEEFHPFLFSQHSKCPYIEFESFDKAVDEFYSKLEGQKIDLKALQQEKQALKKLDNVRKDHEHRLEALHQAQEIDKIKGELIEMNLQIVDRAIQVVRSALANQIDWTEIGLIVKEAQAQGDIVANAIRELKLQTNHVTMLLKNPYLISDEEEEDDEINIEKEETEEPKGKKKKQKNKQLQKLQKNKPLLVDVDLSLSAYANAKKYYDHKRHAARKTQKTVEAAEKAFRSAEKKTKQTLKEVQMVTTIQKARKVYCISLVIESNIIFQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDEPCVWRHRGERKVRGQDEDLETVASSTSKLVSEEMEPLDNGDSSSGEDQEETSETVEEREVVNQIDEEVISIQNDKNRPKEGSAQEESSDDDAADDADADADDDEEEEEDDDDDDDDHDHDNTNDDRKSQRMKSDQEIASKRKDESEMSLNYPDTTIDLSHLQSQRSFQKTVTREDASDVNDSKLHGRRHLSAKERREMKKKKQPNDSTDLDILEDKGKENTLKTEVFPNTSKTVSGPQPMKRGQKSKIKKMKEKYKDQDEEDRELIMKLLGSAGSSKEEKGKKGKKGKTGKTKEEATKKQPQKFRSELRIGDRIKKETPLEAVIHELQEITMDDQPDDKEEQDVDQQGNEENLLDSLTGQPHSEDVLLFAIPVCAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNTFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLNAKKKNN

Target ORF information:

RefSeq Version	XM_012549075.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii nuclear export mediator factor (NEMF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012549075.1

ORF Insert Sequence:

ATGAAGACGC GTTTCAGCTC CGTAGATATT TGCGCTATCC TCTCCGAGTT CAATGCCAGA 
TTATTGGGAA TGAGAGTATA CAATATTTAT GATGTGGATA ATAAGACATA CCTTATACGC 
CTTCAAAAGC CAGACTTTAA AGTTACGCTT CTACTTGAAT CTGGTATACG AATTCACACG 
ACAGAGTTTG AATGGCCAAA GAATATGATG CCATCTAGCT TTGCCATGAA GTGCCGAAAG 
CATTTGAAGA GTCGAAGACT GGTCAGTGTA AAACAGCTTG GTGTGGATCG GATTGTAGAT 
TTTCAGTTTG GAAGTGATGA AGCTGCCTAT CATTTAATCA TTGAGCTTTA TGATAAAGGC 
AATATTGTTC TTACCAACTA TGAATACCTC ATTTTAAATA TCTTGAGGTT TAGAAGTGAT 
GAAGCAGATG ATGTGAAGTT TGCTGTTCGA GAAAAATATC CAATTGATCA TGCTAGGGTA 
ATGGAACCAT TACTTTCACT GGAAAGATTA ACTGAAATAA TATCCAGTGC ACCAAAAGGA 
GAACAATTAA GGAGGATTCT TAATCCATAT CTCCCCTACG GGGCCACTCT GATTGAACAC 
TGTCTTAGAG AAAATGGATT TTCCAGCTAT TTCAGAGTAG ATGAGAAATT TGAAACTGGA 
GATATTGAAA AAGTACTTGT TTGTCTGCAA AAAGCAGAAG ATCATATGAA AACAATGTCC 
AACTTCAGTG GAAAGGGGTA TATCATTCAG AAAAAAGAGA AAAAACCAAG CCTGGAGCCA 
GATAAGCAAT CTGAAGATAT ACTCACATAT GAAGAATTTC ATCCTTTCTT ATTTTCTCAA 
CATTCCAAAT GTCCATATAT AGAATTTGAA TCATTTGACA AGGCAGTGGA TGAATTTTAT 
TCTAAGCTAG AAGGCCAGAA GATAGACTTG AAAGCTTTAC AACAGGAGAA ACAAGCATTG 
AAGAAATTGG ATAATGTCCG TAAGGATCAT GAGCACAGAT TAGAAGCCCT TCACCAAGCT 
CAGGAAATTG ATAAAATCAA AGGAGAACTC ATAGAAATGA ATCTACAGAT AGTTGATAGA 
GCCATCCAAG TAGTTCGGAG TGCTTTAGCC AACCAGATAG ACTGGACAGA AATTGGACTA 
ATTGTAAAAG AGGCCCAAGC TCAAGGAGAT ATTGTTGCAA ATGCAATCAG AGAATTGAAG 
CTGCAAACCA ATCATGTCAC AATGCTGCTA AAAAATCCAT ATCTAATATC AGATGAGGAG 
GAGGAAGATG ATGAAATTAA TATTGAAAAA GAAGAAACAG AAGAACCAAA AGGGAAAAAA 
AAAAAGCAGA AGAACAAACA ACTTCAGAAA CTTCAGAAGA ATAAGCCATT ACTTGTTGAT 
GTTGATCTCA GTTTGTCAGC ATATGCCAAT GCCAAAAAAT ATTATGACCA CAAGAGACAT 
GCTGCCAGAA AAACACAAAA AACTGTTGAA GCTGCTGAGA AGGCATTCAG ATCTGCGGAA 
AAGAAAACAA AACAAACCCT CAAAGAAGTT CAGATGGTTA CCACTATTCA GAAAGCAAGG 
AAAGTGTATT GTATTTCTCT TGTAATTGAA TCTAATATTA TTTTTCAGGT CTCTAAAACA 
GCACCAACTG GAGAATATTT GACAACAGGA AGCTTCATGA TAAGAGGAAA GAAGAATTTT 
CTTCCTCCTT CATATCTTAT GATGGGGTTT AGTTTCCTAT TTAAGGTAGA TGAACCATGT 
GTTTGGAGAC ATCGAGGGGA ACGGAAAGTC AGAGGTCAAG ATGAAGATTT GGAGACAGTG 
GCTAGCAGCA CTAGTAAGCT AGTTTCAGAA GAAATGGAGC CATTAGATAA TGGTGATAGC 
AGCAGTGGAG AGGATCAAGA AGAAACTTCT GAAACTGTAG AAGAAAGGGA AGTAGTAAAT 
CAAATTGATG AAGAGGTCAT TAGCATTCAA AATGACAAAA ACAGGCCAAA AGAAGGATCA 
GCACAAGAAG AGAGCTCAGA TGATGATGCT GCGGATGATG CTGATGCTGA TGCTGATGAT 
GATGAGGAGG AGGAGGAGGA CGATGACGAC GACGATGATG ATCATGATCA TGATAACACC 
AATGACGATA GAAAATCTCA AAGAATGAAA TCAGATCAGG AAATAGCAAG TAAAAGGAAG 
GATGAATCTG AAATGTCATT AAATTATCCA GATACTACAA TTGATCTATC TCACCTTCAG 
TCCCAAAGAT CCTTCCAGAA AACAGTTACT AGAGAAGATG CTTCTGATGT GAATGACAGT 
AAATTACATG GCCGGAGGCA TTTGTCCGCC AAGGAACGAA GGGAAATGAA GAAAAAAAAG 
CAACCAAATG ATTCAACAGA TTTAGATATA TTGGAGGACA AGGGAAAAGA AAATACACTG 
AAGACTGAAG TTTTTCCAAA TACAAGCAAA ACTGTTTCGG GTCCACAACC AATGAAGAGA 
GGACAGAAGA GTAAAATTAA AAAAATGAAA GAAAAATATA AAGATCAAGA TGAGGAAGAT 
CGTGAACTTA TCATGAAGCT CCTTGGATCT GCAGGTTCAA GCAAAGAAGA AAAAGGAAAG 
AAAGGGAAGA AAGGAAAAAC GGGGAAAACA AAAGAAGAGG CAACAAAGAA ACAACCCCAG 
AAGTTCAGAA GTGAACTGAG AATTGGTGAC AGAATTAAGA AAGAAACTCC TCTTGAAGCT 
GTAATTCATG AATTACAAGA AATTACCATG GACGATCAGC CAGATGATAA GGAAGAACAA 
GATGTAGACC AACAAGGAAA TGAGGAGAAC CTGCTGGATT CTTTAACAGG ACAACCCCAC 
TCTGAAGATG TTTTGCTTTT TGCTATACCA GTATGTGCCC CTTATACCAC CATGACCAAC 
TATAAATACA AAGTGAAACT TACTCCTGGA GTCCAAAAAA AAGGAAAAGC GGCTAAAACT 
GCCTTGAATA CTTTCATGCA TTCAAAAGAA GCAACAGCAA GAGAAAAAGA TTTATTTCGC 
AGTGTAAAGG ACACTGATTT ATCAAGAAAT ATTCCTGGAA AAGTGAAAGT ATCTGCTCCC 
AATCTCCTTA ATGCAAAGAA GAAAAATAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEMF ( XM_023503090.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023358858.1 Sarcophilus harrisii nuclear export mediator factor (NEMF), mRNA.

Price & Availability↑

CloneID	OSa149790
Clone ID Related Accession (Same CDS sequence)	XM_023503090.1
Accession Version	XM_023503090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	nuclear export mediator factor NEMF
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816564.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAAGACGC GTTTCAGCTC CGTAGATATT TGCGCTATCC TCTCCGAGTT CAATGCCAGA 
TTATTGGGAA TGAGAGTATA CAATATTTAT GATGTGGATA ATAAGACATA CCTTATACGC 
CTTCAAAAGC CAGACTTTAA AGTTACGCTT CTACTTGAAT CTGGTATACG AATTCACACG 
ACAGAGTTTG AATGGCCAAA GAATATGATG CCATCTAGCT TTGCCATGAA GTGCCGAAAG 
CATTTGAAGA GTCGAAGACT GGTCAGTGTA AAACAGCTTG GTGTGGATCG GATTGTAGAT 
TTTCAGTTTG GAAGTGATGA AGCTGCCTAT CATTTAATCA TTGAGCTTTA TGATAAAGGC 
AATATTGTTC TTACCAACTA TGAATACCTC ATTTTAAATA TCTTGAGGTT TAGAAGTGAT 
GAAGCAGATG ATGTGAAGTT TGCTGTTCGA GAAAAATATC CAATTGATCA TGCTAGGGTA 
ATGGAACCAT TACTTTCACT GGAAAGATTA ACTGAAATAA TATCCAGTGC ACCAAAAGGA 
GAACAATTAA GGAGGATTCT TAATCCATAT CTCCCCTACG GGGCCACTCT GATTGAACAC 
TGTCTTAGAG AAAATGGATT TTCCAGCTAT TTCAGAGTAG ATGAGAAATT TGAAACTGGA 
GATATTGAAA AAGTACTTGT TTGTCTGCAA AAAGCAGAAG ATCATATGAA AACAATGTCC 
AACTTCAGTG GAAAGGGGTA TATCATTCAG AAAAAAGAGA AAAAACCAAG CCTGGAGCCA 
GATAAGCAAT CTGAAGATAT ACTCACATAT GAAGAATTTC ATCCTTTCTT ATTTTCTCAA 
CATTCCAAAT GTCCATATAT AGAATTTGAA TCATTTGACA AGGCAGTGGA TGAATTTTAT 
TCTAAGCTAG AAGGCCAGAA GATAGACTTG AAAGCTTTAC AACAGGAGAA ACAAGCATTG 
AAGAAATTGG ATAATGTCCG TAAGGATCAT GAGCACAGAT TAGAAGCCCT TCACCAAGCT 
CAGGAAATTG ATAAAATCAA AGGAGAACTC ATAGAAATGA ATCTACAGAT AGTTGATAGA 
GCCATCCAAG TAGTTCGGAG TGCTTTAGCC AACCAGATAG ACTGGACAGA AATTGGACTA 
ATTGTAAAAG AGGCCCAAGC TCAAGGAGAT ATTGTTGCAA ATGCAATCAG AGAATTGAAG 
CTGCAAACCA ATCATGTCAC AATGCTGCTA AAAAATCCAT ATCTAATATC AGATGAGGAG 
GAGGAAGATG ATGAAATTAA TATTGAAAAA GAAGAAACAG AAGAACCAAA AGGGAAAAAA 
AAAAAGCAGA AGAACAAACA ACTTCAGAAA CTTCAGAAGA ATAAGCCATT ACTTGTTGAT 
GTTGATCTCA GTTTGTCAGC ATATGCCAAT GCCAAAAAAT ATTATGACCA CAAGAGACAT 
GCTGCCAGAA AAACACAAAA AACTGTTGAA GCTGCTGAGA AGGCATTCAG ATCTGCGGAA 
AAGAAAACAA AACAAACCCT CAAAGAAGTT CAGATGGTTA CCACTATTCA GAAAGCAAGG 
AAAGTGTATT GTAAATGCTT GTTTCCATCT ATTCCCCTTT TCATTAATTC TTTCAGTATT 
TCTCTTGTAA TTGAATCTAA TATTATTTTT CAGGTCTCTA AAACAGCACC AACTGGAGAA 
TATTTGACAA CAGGAAGCTT CATGATAAGA GGAAAGAAGA ATTTTCTTCC TCCTTCATAT 
CTTATGATGG GGTTTAGTTT CCTATTTAAG GTAGATGAAC CATGTGTTTG GAGACATCGA 
GGGGAACGGA AAGTCAGAGG TCAAGATGAA GATTTGGAGA CAGTGGCTAG CAGCACTAGT 
AAGCTAGTTT CAGAAGAAAT GGAGCCATTA GATAATGGTG ATAGCAGCAG TGGAGAGGAT 
CAAGAAGAAA CTTCTGAAAC TGTAGAAGAA AGGGAAGTAG TAAATCAAAT TGATGAAGAG 
GTCATTAGCA TTCAAAATGA CAAAAACAGG CCAAAAGAAG GATCAGCACA AGAAGAGAGC 
TCAGATGATG ATGCTGCGGA TGATGCTGAT GCTGATGCTG ATGATGATGA GGAGGAGGAG 
GAGGACGATG ACGACGACGA TGATGATCAT GATCATGATA ACACCAATGA CGATAGAAAA 
TCTCAAAGAA TGAAATCAGA TCAGGAAATA GCAAGTAAAA GGAAGGATGA ATCTGAAATG 
TCATTAAATT ATCCAGATAC TACAATTGAT CTATCTCACC TTCAGTCCCA AAGATCCTTC 
CAGAAAACAG TTACTAGAGA AGATGCTTCT GATGTGAATG ACAGTAAATT ACATGGCCGG 
AGGCATTTGT CCGCCAAGGA ACGAAGGGAA ATGAAGAAAA AAAAGCAACC AAATGATTCA 
ACAGATTTAG ATATATTGGA GGACAAGGGA AAAGAAAATA CACTGAAGAC TGAAGTTTTT 
CCAAATACAA GCAAAACTGT TTCGGGTCCA CAACCAATGA AGAGAGGACA GAAGAGTAAA 
ATTAAAAAAA TGAAAGAAAA ATATAAAGAT CAAGATGAGG AAGATCGTGA ACTTATCATG 
AAGCTCCTTG GATCTGCAGG TTCAAGCAAA GAAGAAAAAG GAAAGAAAGG GAAGAAAGGA 
AAAACGGGGA AAACAAAAGA AGAGGCAACA AAGAAACAAC CCCAGAAGTT CAGAAGTGAA 
CTGAGAATTG GTGACAGAAT TAAGAAAGAA ACTCCTCTTG AAGCTGTAAT TCATGAATTA 
CAAGAAATTA CCATGGACGA TCAGCCAGAT GATAAGGAAG AACAAGATGT AGACCAACAA 
GGAAATGAGG AGAACCTGCT GGATTCTTTA ACAGGACAAC CCCACTCTGA AGATGTTTTG 
CTTTTTGCTA TACCAGTATG TGCCCCTTAT ACCACCATGA CCAACTATAA ATACAAAGTG 
AAACTTACTC CTGGAGTCCA AAAAAAAGGA AAAGCGGCTA AAACTGCCTT GAATACTTTC 
ATGCATTCAA AAGAAGCAAC AGCAAGAGAA AAAGATTTAT TTCGCAGTGT AAAGGACACT 
GATTTATCAA GAAATATTCC TGGAAAAGTG AAAGTATCTG CTCCCAATCT CCTTAATGCA 
AAGAAGAAAA ATAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023358858.1
CDS	174..3311
Translation	MKTRFSSVDICAILSEFNARLLGMRVYNIYDVDNKTYLIRLQKPDFKVTLLLESGIRIHTTEFEWPKNMMPSSFAMKCRKHLKSRRLVSVKQLGVDRIVDFQFGSDEAAYHLIIELYDKGNIVLTNYEYLILNILRFRSDEADDVKFAVREKYPIDHARVMEPLLSLERLTEIISSAPKGEQLRRILNPYLPYGATLIEHCLRENGFSSYFRVDEKFETGDIEKVLVCLQKAEDHMKTMSNFSGKGYIIQKKEKKPSLEPDKQSEDILTYEEFHPFLFSQHSKCPYIEFESFDKAVDEFYSKLEGQKIDLKALQQEKQALKKLDNVRKDHEHRLEALHQAQEIDKIKGELIEMNLQIVDRAIQVVRSALANQIDWTEIGLIVKEAQAQGDIVANAIRELKLQTNHVTMLLKNPYLISDEEEEDDEINIEKEETEEPKGKKKKQKNKQLQKLQKNKPLLVDVDLSLSAYANAKKYYDHKRHAARKTQKTVEAAEKAFRSAEKKTKQTLKEVQMVTTIQKARKVYCKCLFPSIPLFINSFSISLVIESNIIFQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDEPCVWRHRGERKVRGQDEDLETVASSTSKLVSEEMEPLDNGDSSSGEDQEETSETVEEREVVNQIDEEVISIQNDKNRPKEGSAQEESSDDDAADDADADADDDEEEEEDDDDDDDDHDHDNTNDDRKSQRMKSDQEIASKRKDESEMSLNYPDTTIDLSHLQSQRSFQKTVTREDASDVNDSKLHGRRHLSAKERREMKKKKQPNDSTDLDILEDKGKENTLKTEVFPNTSKTVSGPQPMKRGQKSKIKKMKEKYKDQDEEDRELIMKLLGSAGSSKEEKGKKGKKGKTGKTKEEATKKQPQKFRSELRIGDRIKKETPLEAVIHELQEITMDDQPDDKEEQDVDQQGNEENLLDSLTGQPHSEDVLLFAIPVCAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNTFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLNAKKKNN

Target ORF information:

RefSeq Version	XM_023503090.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii nuclear export mediator factor (NEMF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023503090.1

ORF Insert Sequence:

ATGAAGACGC GTTTCAGCTC CGTAGATATT TGCGCTATCC TCTCCGAGTT CAATGCCAGA 
TTATTGGGAA TGAGAGTATA CAATATTTAT GATGTGGATA ATAAGACATA CCTTATACGC 
CTTCAAAAGC CAGACTTTAA AGTTACGCTT CTACTTGAAT CTGGTATACG AATTCACACG 
ACAGAGTTTG AATGGCCAAA GAATATGATG CCATCTAGCT TTGCCATGAA GTGCCGAAAG 
CATTTGAAGA GTCGAAGACT GGTCAGTGTA AAACAGCTTG GTGTGGATCG GATTGTAGAT 
TTTCAGTTTG GAAGTGATGA AGCTGCCTAT CATTTAATCA TTGAGCTTTA TGATAAAGGC 
AATATTGTTC TTACCAACTA TGAATACCTC ATTTTAAATA TCTTGAGGTT TAGAAGTGAT 
GAAGCAGATG ATGTGAAGTT TGCTGTTCGA GAAAAATATC CAATTGATCA TGCTAGGGTA 
ATGGAACCAT TACTTTCACT GGAAAGATTA ACTGAAATAA TATCCAGTGC ACCAAAAGGA 
GAACAATTAA GGAGGATTCT TAATCCATAT CTCCCCTACG GGGCCACTCT GATTGAACAC 
TGTCTTAGAG AAAATGGATT TTCCAGCTAT TTCAGAGTAG ATGAGAAATT TGAAACTGGA 
GATATTGAAA AAGTACTTGT TTGTCTGCAA AAAGCAGAAG ATCATATGAA AACAATGTCC 
AACTTCAGTG GAAAGGGGTA TATCATTCAG AAAAAAGAGA AAAAACCAAG CCTGGAGCCA 
GATAAGCAAT CTGAAGATAT ACTCACATAT GAAGAATTTC ATCCTTTCTT ATTTTCTCAA 
CATTCCAAAT GTCCATATAT AGAATTTGAA TCATTTGACA AGGCAGTGGA TGAATTTTAT 
TCTAAGCTAG AAGGCCAGAA GATAGACTTG AAAGCTTTAC AACAGGAGAA ACAAGCATTG 
AAGAAATTGG ATAATGTCCG TAAGGATCAT GAGCACAGAT TAGAAGCCCT TCACCAAGCT 
CAGGAAATTG ATAAAATCAA AGGAGAACTC ATAGAAATGA ATCTACAGAT AGTTGATAGA 
GCCATCCAAG TAGTTCGGAG TGCTTTAGCC AACCAGATAG ACTGGACAGA AATTGGACTA 
ATTGTAAAAG AGGCCCAAGC TCAAGGAGAT ATTGTTGCAA ATGCAATCAG AGAATTGAAG 
CTGCAAACCA ATCATGTCAC AATGCTGCTA AAAAATCCAT ATCTAATATC AGATGAGGAG 
GAGGAAGATG ATGAAATTAA TATTGAAAAA GAAGAAACAG AAGAACCAAA AGGGAAAAAA 
AAAAAGCAGA AGAACAAACA ACTTCAGAAA CTTCAGAAGA ATAAGCCATT ACTTGTTGAT 
GTTGATCTCA GTTTGTCAGC ATATGCCAAT GCCAAAAAAT ATTATGACCA CAAGAGACAT 
GCTGCCAGAA AAACACAAAA AACTGTTGAA GCTGCTGAGA AGGCATTCAG ATCTGCGGAA 
AAGAAAACAA AACAAACCCT CAAAGAAGTT CAGATGGTTA CCACTATTCA GAAAGCAAGG 
AAAGTGTATT GTAAATGCTT GTTTCCATCT ATTCCCCTTT TCATTAATTC TTTCAGTATT 
TCTCTTGTAA TTGAATCTAA TATTATTTTT CAGGTCTCTA AAACAGCACC AACTGGAGAA 
TATTTGACAA CAGGAAGCTT CATGATAAGA GGAAAGAAGA ATTTTCTTCC TCCTTCATAT 
CTTATGATGG GGTTTAGTTT CCTATTTAAG GTAGATGAAC CATGTGTTTG GAGACATCGA 
GGGGAACGGA AAGTCAGAGG TCAAGATGAA GATTTGGAGA CAGTGGCTAG CAGCACTAGT 
AAGCTAGTTT CAGAAGAAAT GGAGCCATTA GATAATGGTG ATAGCAGCAG TGGAGAGGAT 
CAAGAAGAAA CTTCTGAAAC TGTAGAAGAA AGGGAAGTAG TAAATCAAAT TGATGAAGAG 
GTCATTAGCA TTCAAAATGA CAAAAACAGG CCAAAAGAAG GATCAGCACA AGAAGAGAGC 
TCAGATGATG ATGCTGCGGA TGATGCTGAT GCTGATGCTG ATGATGATGA GGAGGAGGAG 
GAGGACGATG ACGACGACGA TGATGATCAT GATCATGATA ACACCAATGA CGATAGAAAA 
TCTCAAAGAA TGAAATCAGA TCAGGAAATA GCAAGTAAAA GGAAGGATGA ATCTGAAATG 
TCATTAAATT ATCCAGATAC TACAATTGAT CTATCTCACC TTCAGTCCCA AAGATCCTTC 
CAGAAAACAG TTACTAGAGA AGATGCTTCT GATGTGAATG ACAGTAAATT ACATGGCCGG 
AGGCATTTGT CCGCCAAGGA ACGAAGGGAA ATGAAGAAAA AAAAGCAACC AAATGATTCA 
ACAGATTTAG ATATATTGGA GGACAAGGGA AAAGAAAATA CACTGAAGAC TGAAGTTTTT 
CCAAATACAA GCAAAACTGT TTCGGGTCCA CAACCAATGA AGAGAGGACA GAAGAGTAAA 
ATTAAAAAAA TGAAAGAAAA ATATAAAGAT CAAGATGAGG AAGATCGTGA ACTTATCATG 
AAGCTCCTTG GATCTGCAGG TTCAAGCAAA GAAGAAAAAG GAAAGAAAGG GAAGAAAGGA 
AAAACGGGGA AAACAAAAGA AGAGGCAACA AAGAAACAAC CCCAGAAGTT CAGAAGTGAA 
CTGAGAATTG GTGACAGAAT TAAGAAAGAA ACTCCTCTTG AAGCTGTAAT TCATGAATTA 
CAAGAAATTA CCATGGACGA TCAGCCAGAT GATAAGGAAG AACAAGATGT AGACCAACAA 
GGAAATGAGG AGAACCTGCT GGATTCTTTA ACAGGACAAC CCCACTCTGA AGATGTTTTG 
CTTTTTGCTA TACCAGTATG TGCCCCTTAT ACCACCATGA CCAACTATAA ATACAAAGTG 
AAACTTACTC CTGGAGTCCA AAAAAAAGGA AAAGCGGCTA AAACTGCCTT GAATACTTTC 
ATGCATTCAA AAGAAGCAAC AGCAAGAGAA AAAGATTTAT TTCGCAGTGT AAAGGACACT 
GATTTATCAA GAAATATTCC TGGAAAAGTG AAAGTATCTG CTCCCAATCT CCTTAATGCA 
AAGAAGAAAA ATAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEMF ( XM_031952816.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031808676.1 Sarcophilus harrisii nuclear export mediator factor (NEMF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa225339
Clone ID Related Accession (Same CDS sequence)	XM_031952816.1 , XM_031952817.1
Accession Version	XM_031952816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	nuclear export mediator factor NEMF isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGC GTTTCAGCTC CGTAGATATT TGCGCTATCC TCTCCGAGTT CAATGCCAGA 
TTATTGGGAA TGAGAGTATA CAATATTTAT GATGTGGATA ATAAGACATA CCTTATACGC 
CTTCAAAAGC CAGACTTTAA AGTTACGCTT CTACTTGAAT CTGGTATACG AATTCACACG 
ACAGAGTTTG AATGGCCAAA GAATATGATG CCATCTAGCT TTGCCATGAA GTGCCGAAAG 
CATTTGAAGA GTCGAAGACT GGTCAGTGTA AAACAGCTTG GTGTGGATCG GATTGTAGAT 
TTTCAGTTTG GAAGTGATGA AGCTGCCTAT CATTTAATCA TTGAGCTTTA TGATAAAGGC 
AATATTGTTC TTACCAACTA TGAATACCTC ATTTTAAATA TCTTGAGGTT TAGAAGTGAT 
GAAGCAGATG ATGTGAAGTT TGCTGTTCGA GAAAAATATC CAATTGATCA TGCTAGGGTA 
ATGGAACCAT TACTTTCACT GGAAAGATTA ACTGAAATAA TATCCAGTGC ACCAAAAGGA 
GAACAATTAA GGAGGATTCT TAATCCATAT CTCCCCTACG GGGCCACTCT GATTGAACAC 
TGTCTTAGAG AAAATGGATT TTCCAGCTAT TTCAGAGTAG ATGAGAAATT TGAAACTGGA 
GATATTGAAA AAGTACTTGT TTGTCTGCAA AAAGCAGAAG ATCATATGAA AACAATGTCC 
AACTTCAGTG GAAAGGGGTA TATCATTCAG AAAAAAGAGA AAAAACCAAG CCTGGAGCCA 
GATAAGCAAT CTGAAGATAT ACTCACATAT GAAGAATTTC ATCCTTTCTT ATTTTCTCAA 
CATTCCAAAT GTCCATATAT AGAATTTGAA TCATTTGACA AGGCAGTGGA TGAATTTTAT 
TCTAAGCTAG AAGGCCAGAA GATAGACTTG AAAGCTTTAC AACAGGAGAA ACAAGCATTG 
AAGAAATTGG ATAATGTCCG TAAGGATCAT GAGCACAGAT TAGAAGCCCT TCACCAAGCT 
CAGGAAATTG ATAAAATCAA AGGAGAACTC ATAGAAATGA ATCTACAGAT AGTTGATAGA 
GCCATCCAAG TAGTTCGGAG TGCTTTAGCC AACCAGATAG ACTGGACAGA AATTGGACTA 
ATTGTAAAAG AGGCCCAAGC TCAAGGAGAT ATTGTTGCAA ATGCAATCAG AGAATTGAAG 
CTGCAAACCA ATCATGTCAC AATGCTGCTA AAAAATCCAT ATCTAATATC AGATGAGGAG 
GAGGAAGATG ATGAAATTAA TATTGAAAAA GAAGAAACAG AAGAACCAAA AGGGAAAAAA 
AAAAAGCAGA AGAACAAACA ACTTCAGAAA CTTCAGAAGA ATAAGCCATT ACTTGTTGAT 
GTTGATCTCA GTTTGTCAGC ATATGCCAAT GCCAAAAAAT ATTATGACCA CAAGAGACAT 
GCTGCCAGAA AAACACAAAA AACTGTTGAA GCTGCTGAGA AGGCATTCAG ATCTGCGGAA 
AAGAAAACAA AACAAACCCT CAAAGAAGTT CAGATGGTTA CCACTATTCA GAAAGCAAGG 
AAAGTGTATT GGTTTGAGAA GTTTTTATGG TTTATTAGCT CTGAGAACTA TCTCATTATA 
GGTGGAAGAG ATCAACAACA AAATGAAATG ATTGTGAAGC GATATTTGAC ACCAGGAGAT 
ATTTATGTTC ATGCTGATCT TCATGGTGCA ACAAGCTGTG TAATTAAGAA TCCAACGGGA 
GAACCAATCC CCCCACGGAC TTTGACAGAA GCTGGCACGA TGGCATTGTG TTATAGTGCT 
GCCTGGGATG CTCGAGTTAT CACCAGTGCT TGGTGGGTGT ATCATCATCA GGTCTCTAAA 
ACAGCACCAA CTGGAGAATA TTTGACAACA GGAAGCTTCA TGATAAGAGG AAAGAAGAAT 
TTTCTTCCTC CTTCATATCT TATGATGGGG TTTAGTTTCC TATTTAAGGT AGATGAACCA 
TGTGTTTGGA GACATCGAGG GGAACGGAAA GTCAGAGGTC AAGATGAAGA TTTGGAGACA 
GTGGCTAGCA GCACTAGTAA GCTAGTTTCA GAAGAAATGG AGCCATTAGA TAATGGTGAT 
AGCAGCAGTG GAGAGGATCA AGAAGAAACT TCTGAAACTG TAGAAGAAAG GGAAGTAGTA 
AATCAAATTG ATGAAGAGGT CATTAGCATT CAAAATGACA AAAACAGGCC AAAAGAAGGA 
TCAGCACAAG AAGAGAGCTC AGATGATGAT GCTGCGGATG ATGCTGATGC TGATGCTGAT 
GATGATGAGG AGGAGGAGGA GGACGATGAC GACGACGATG ATGATCATGA TCATGATAAC 
ACCAATGACG ATAGAAAATC TCAAAGAATG AAATCAGATC AGGAAATAGC AAGTAAAAGG 
AAGGATGAAT CTGAAATGTC ATTAAATTAT CCAGATACTA CAATTGATCT ATCTCACCTT 
CAGTCCCAAA GATCCTTCCA GAAAACAGTT ACTAGAGAAG ATGCTTCTGA TGTGAATGAC 
AGTAAATTAC ATGGCCGGAG GCATTTGTCC GCCAAGGAAC GAAGGGAAAT GAAGAAAAAA 
AAGCAACCAA ATGATTCAAC AGATTTAGAT ATATTGGAGG ACAAGGGAAA AGAAAATACA 
CTGAAGACTG AAGTTTTTCC AAATACAAGC AAAACTGTTT CGGGTCCACA ACCAATGAAG 
AGAGGACAGA AGAGTAAAAT TAAAAAAATG AAAGAAAAAT ATAAAGATCA AGATGAGGAA 
GATCGTGAAC TTATCATGAA GCTCCTTGGA TCTGCAGGTT CAAGCAAAGA AGAAAAAGGA 
AAGAAAGGGA AGAAAGGAAA AACGGGGAAA ACAAAAGAAG AGGCAACAAA GAAACAACCC 
CAGAAGTTCA GAAGTGAACT GAGAATTGGT GACAGAATTA AGAAAGAAAC TCCTCTTGAA 
GCTGTAATTC ATGAATTACA AGAAATTACC ATGGACGATC AGCCAGATGA TAAGCAGGAA 
GAACAAGATG TAGACCAACA AGGAAATGAG GAGAACCTGC TGGATTCTTT AACAGGACAA 
CCCCACTCTG AAGATGTTTT GCTTTTTGCT ATACCAGTAT GTGCCCCTTA TACCACCATG 
ACCAACTATA AATACAAAGT GAAACTTACT CCTGGAGTCC AAAAAAAAGG AAAAGCGGCT 
AAAACTGCCT TGAATACTTT CATGCATTCA AAAGAAGCAA CAGCAAGAGA AAAAGATTTA 
TTTCGCAGTG TAAAGGACAC TGATTTATCA AGAAATATTC CTGGAAAAGT GAAAGTATCT 
GCTCCCAATC TCCTTAATGC AAAGAAGAAA AATAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031808676.1
CDS	108..3446
Translation	MKTRFSSVDICAILSEFNARLLGMRVYNIYDVDNKTYLIRLQKPDFKVTLLLESGIRIHTTEFEWPKNMMPSSFAMKCRKHLKSRRLVSVKQLGVDRIVDFQFGSDEAAYHLIIELYDKGNIVLTNYEYLILNILRFRSDEADDVKFAVREKYPIDHARVMEPLLSLERLTEIISSAPKGEQLRRILNPYLPYGATLIEHCLRENGFSSYFRVDEKFETGDIEKVLVCLQKAEDHMKTMSNFSGKGYIIQKKEKKPSLEPDKQSEDILTYEEFHPFLFSQHSKCPYIEFESFDKAVDEFYSKLEGQKIDLKALQQEKQALKKLDNVRKDHEHRLEALHQAQEIDKIKGELIEMNLQIVDRAIQVVRSALANQIDWTEIGLIVKEAQAQGDIVANAIRELKLQTNHVTMLLKNPYLISDEEEEDDEINIEKEETEEPKGKKKKQKNKQLQKLQKNKPLLVDVDLSLSAYANAKKYYDHKRHAARKTQKTVEAAEKAFRSAEKKTKQTLKEVQMVTTIQKARKVYWFEKFLWFISSENYLIIGGRDQQQNEMIVKRYLTPGDIYVHADLHGATSCVIKNPTGEPIPPRTLTEAGTMALCYSAAWDARVITSAWWVYHHQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDEPCVWRHRGERKVRGQDEDLETVASSTSKLVSEEMEPLDNGDSSSGEDQEETSETVEEREVVNQIDEEVISIQNDKNRPKEGSAQEESSDDDAADDADADADDDEEEEEDDDDDDDDHDHDNTNDDRKSQRMKSDQEIASKRKDESEMSLNYPDTTIDLSHLQSQRSFQKTVTREDASDVNDSKLHGRRHLSAKERREMKKKKQPNDSTDLDILEDKGKENTLKTEVFPNTSKTVSGPQPMKRGQKSKIKKMKEKYKDQDEEDRELIMKLLGSAGSSKEEKGKKGKKGKTGKTKEEATKKQPQKFRSELRIGDRIKKETPLEAVIHELQEITMDDQPDDKQEEQDVDQQGNEENLLDSLTGQPHSEDVLLFAIPVCAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNTFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLNAKKKNN

Target ORF information:

RefSeq Version	XM_031952816.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii nuclear export mediator factor (NEMF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031952816.1

ORF Insert Sequence:

ATGAAGACGC GTTTCAGCTC CGTAGATATT TGCGCTATCC TCTCCGAGTT CAATGCCAGA 
TTATTGGGAA TGAGAGTATA CAATATTTAT GATGTGGATA ATAAGACATA CCTTATACGC 
CTTCAAAAGC CAGACTTTAA AGTTACGCTT CTACTTGAAT CTGGTATACG AATTCACACG 
ACAGAGTTTG AATGGCCAAA GAATATGATG CCATCTAGCT TTGCCATGAA GTGCCGAAAG 
CATTTGAAGA GTCGAAGACT GGTCAGTGTA AAACAGCTTG GTGTGGATCG GATTGTAGAT 
TTTCAGTTTG GAAGTGATGA AGCTGCCTAT CATTTAATCA TTGAGCTTTA TGATAAAGGC 
AATATTGTTC TTACCAACTA TGAATACCTC ATTTTAAATA TCTTGAGGTT TAGAAGTGAT 
GAAGCAGATG ATGTGAAGTT TGCTGTTCGA GAAAAATATC CAATTGATCA TGCTAGGGTA 
ATGGAACCAT TACTTTCACT GGAAAGATTA ACTGAAATAA TATCCAGTGC ACCAAAAGGA 
GAACAATTAA GGAGGATTCT TAATCCATAT CTCCCCTACG GGGCCACTCT GATTGAACAC 
TGTCTTAGAG AAAATGGATT TTCCAGCTAT TTCAGAGTAG ATGAGAAATT TGAAACTGGA 
GATATTGAAA AAGTACTTGT TTGTCTGCAA AAAGCAGAAG ATCATATGAA AACAATGTCC 
AACTTCAGTG GAAAGGGGTA TATCATTCAG AAAAAAGAGA AAAAACCAAG CCTGGAGCCA 
GATAAGCAAT CTGAAGATAT ACTCACATAT GAAGAATTTC ATCCTTTCTT ATTTTCTCAA 
CATTCCAAAT GTCCATATAT AGAATTTGAA TCATTTGACA AGGCAGTGGA TGAATTTTAT 
TCTAAGCTAG AAGGCCAGAA GATAGACTTG AAAGCTTTAC AACAGGAGAA ACAAGCATTG 
AAGAAATTGG ATAATGTCCG TAAGGATCAT GAGCACAGAT TAGAAGCCCT TCACCAAGCT 
CAGGAAATTG ATAAAATCAA AGGAGAACTC ATAGAAATGA ATCTACAGAT AGTTGATAGA 
GCCATCCAAG TAGTTCGGAG TGCTTTAGCC AACCAGATAG ACTGGACAGA AATTGGACTA 
ATTGTAAAAG AGGCCCAAGC TCAAGGAGAT ATTGTTGCAA ATGCAATCAG AGAATTGAAG 
CTGCAAACCA ATCATGTCAC AATGCTGCTA AAAAATCCAT ATCTAATATC AGATGAGGAG 
GAGGAAGATG ATGAAATTAA TATTGAAAAA GAAGAAACAG AAGAACCAAA AGGGAAAAAA 
AAAAAGCAGA AGAACAAACA ACTTCAGAAA CTTCAGAAGA ATAAGCCATT ACTTGTTGAT 
GTTGATCTCA GTTTGTCAGC ATATGCCAAT GCCAAAAAAT ATTATGACCA CAAGAGACAT 
GCTGCCAGAA AAACACAAAA AACTGTTGAA GCTGCTGAGA AGGCATTCAG ATCTGCGGAA 
AAGAAAACAA AACAAACCCT CAAAGAAGTT CAGATGGTTA CCACTATTCA GAAAGCAAGG 
AAAGTGTATT GGTTTGAGAA GTTTTTATGG TTTATTAGCT CTGAGAACTA TCTCATTATA 
GGTGGAAGAG ATCAACAACA AAATGAAATG ATTGTGAAGC GATATTTGAC ACCAGGAGAT 
ATTTATGTTC ATGCTGATCT TCATGGTGCA ACAAGCTGTG TAATTAAGAA TCCAACGGGA 
GAACCAATCC CCCCACGGAC TTTGACAGAA GCTGGCACGA TGGCATTGTG TTATAGTGCT 
GCCTGGGATG CTCGAGTTAT CACCAGTGCT TGGTGGGTGT ATCATCATCA GGTCTCTAAA 
ACAGCACCAA CTGGAGAATA TTTGACAACA GGAAGCTTCA TGATAAGAGG AAAGAAGAAT 
TTTCTTCCTC CTTCATATCT TATGATGGGG TTTAGTTTCC TATTTAAGGT AGATGAACCA 
TGTGTTTGGA GACATCGAGG GGAACGGAAA GTCAGAGGTC AAGATGAAGA TTTGGAGACA 
GTGGCTAGCA GCACTAGTAA GCTAGTTTCA GAAGAAATGG AGCCATTAGA TAATGGTGAT 
AGCAGCAGTG GAGAGGATCA AGAAGAAACT TCTGAAACTG TAGAAGAAAG GGAAGTAGTA 
AATCAAATTG ATGAAGAGGT CATTAGCATT CAAAATGACA AAAACAGGCC AAAAGAAGGA 
TCAGCACAAG AAGAGAGCTC AGATGATGAT GCTGCGGATG ATGCTGATGC TGATGCTGAT 
GATGATGAGG AGGAGGAGGA GGACGATGAC GACGACGATG ATGATCATGA TCATGATAAC 
ACCAATGACG ATAGAAAATC TCAAAGAATG AAATCAGATC AGGAAATAGC AAGTAAAAGG 
AAGGATGAAT CTGAAATGTC ATTAAATTAT CCAGATACTA CAATTGATCT ATCTCACCTT 
CAGTCCCAAA GATCCTTCCA GAAAACAGTT ACTAGAGAAG ATGCTTCTGA TGTGAATGAC 
AGTAAATTAC ATGGCCGGAG GCATTTGTCC GCCAAGGAAC GAAGGGAAAT GAAGAAAAAA 
AAGCAACCAA ATGATTCAAC AGATTTAGAT ATATTGGAGG ACAAGGGAAA AGAAAATACA 
CTGAAGACTG AAGTTTTTCC AAATACAAGC AAAACTGTTT CGGGTCCACA ACCAATGAAG 
AGAGGACAGA AGAGTAAAAT TAAAAAAATG AAAGAAAAAT ATAAAGATCA AGATGAGGAA 
GATCGTGAAC TTATCATGAA GCTCCTTGGA TCTGCAGGTT CAAGCAAAGA AGAAAAAGGA 
AAGAAAGGGA AGAAAGGAAA AACGGGGAAA ACAAAAGAAG AGGCAACAAA GAAACAACCC 
CAGAAGTTCA GAAGTGAACT GAGAATTGGT GACAGAATTA AGAAAGAAAC TCCTCTTGAA 
GCTGTAATTC ATGAATTACA AGAAATTACC ATGGACGATC AGCCAGATGA TAAGCAGGAA 
GAACAAGATG TAGACCAACA AGGAAATGAG GAGAACCTGC TGGATTCTTT AACAGGACAA 
CCCCACTCTG AAGATGTTTT GCTTTTTGCT ATACCAGTAT GTGCCCCTTA TACCACCATG 
ACCAACTATA AATACAAAGT GAAACTTACT CCTGGAGTCC AAAAAAAAGG AAAAGCGGCT 
AAAACTGCCT TGAATACTTT CATGCATTCA AAAGAAGCAA CAGCAAGAGA AAAAGATTTA 
TTTCGCAGTG TAAAGGACAC TGATTTATCA AGAAATATTC CTGGAAAAGT GAAAGTATCT 
GCTCCCAATC TCCTTAATGC AAAGAAGAAA AATAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEMF ( XM_031952817.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031808677.1 Sarcophilus harrisii nuclear export mediator factor (NEMF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa225339
Clone ID Related Accession (Same CDS sequence)	XM_031952816.1 , XM_031952817.1
Accession Version	XM_031952817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	nuclear export mediator factor NEMF isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGC GTTTCAGCTC CGTAGATATT TGCGCTATCC TCTCCGAGTT CAATGCCAGA 
TTATTGGGAA TGAGAGTATA CAATATTTAT GATGTGGATA ATAAGACATA CCTTATACGC 
CTTCAAAAGC CAGACTTTAA AGTTACGCTT CTACTTGAAT CTGGTATACG AATTCACACG 
ACAGAGTTTG AATGGCCAAA GAATATGATG CCATCTAGCT TTGCCATGAA GTGCCGAAAG 
CATTTGAAGA GTCGAAGACT GGTCAGTGTA AAACAGCTTG GTGTGGATCG GATTGTAGAT 
TTTCAGTTTG GAAGTGATGA AGCTGCCTAT CATTTAATCA TTGAGCTTTA TGATAAAGGC 
AATATTGTTC TTACCAACTA TGAATACCTC ATTTTAAATA TCTTGAGGTT TAGAAGTGAT 
GAAGCAGATG ATGTGAAGTT TGCTGTTCGA GAAAAATATC CAATTGATCA TGCTAGGGTA 
ATGGAACCAT TACTTTCACT GGAAAGATTA ACTGAAATAA TATCCAGTGC ACCAAAAGGA 
GAACAATTAA GGAGGATTCT TAATCCATAT CTCCCCTACG GGGCCACTCT GATTGAACAC 
TGTCTTAGAG AAAATGGATT TTCCAGCTAT TTCAGAGTAG ATGAGAAATT TGAAACTGGA 
GATATTGAAA AAGTACTTGT TTGTCTGCAA AAAGCAGAAG ATCATATGAA AACAATGTCC 
AACTTCAGTG GAAAGGGGTA TATCATTCAG AAAAAAGAGA AAAAACCAAG CCTGGAGCCA 
GATAAGCAAT CTGAAGATAT ACTCACATAT GAAGAATTTC ATCCTTTCTT ATTTTCTCAA 
CATTCCAAAT GTCCATATAT AGAATTTGAA TCATTTGACA AGGCAGTGGA TGAATTTTAT 
TCTAAGCTAG AAGGCCAGAA GATAGACTTG AAAGCTTTAC AACAGGAGAA ACAAGCATTG 
AAGAAATTGG ATAATGTCCG TAAGGATCAT GAGCACAGAT TAGAAGCCCT TCACCAAGCT 
CAGGAAATTG ATAAAATCAA AGGAGAACTC ATAGAAATGA ATCTACAGAT AGTTGATAGA 
GCCATCCAAG TAGTTCGGAG TGCTTTAGCC AACCAGATAG ACTGGACAGA AATTGGACTA 
ATTGTAAAAG AGGCCCAAGC TCAAGGAGAT ATTGTTGCAA ATGCAATCAG AGAATTGAAG 
CTGCAAACCA ATCATGTCAC AATGCTGCTA AAAAATCCAT ATCTAATATC AGATGAGGAG 
GAGGAAGATG ATGAAATTAA TATTGAAAAA GAAGAAACAG AAGAACCAAA AGGGAAAAAA 
AAAAAGCAGA AGAACAAACA ACTTCAGAAA CTTCAGAAGA ATAAGCCATT ACTTGTTGAT 
GTTGATCTCA GTTTGTCAGC ATATGCCAAT GCCAAAAAAT ATTATGACCA CAAGAGACAT 
GCTGCCAGAA AAACACAAAA AACTGTTGAA GCTGCTGAGA AGGCATTCAG ATCTGCGGAA 
AAGAAAACAA AACAAACCCT CAAAGAAGTT CAGATGGTTA CCACTATTCA GAAAGCAAGG 
AAAGTGTATT GGTTTGAGAA GTTTTTATGG TTTATTAGCT CTGAGAACTA TCTCATTATA 
GGTGGAAGAG ATCAACAACA AAATGAAATG ATTGTGAAGC GATATTTGAC ACCAGGAGAT 
ATTTATGTTC ATGCTGATCT TCATGGTGCA ACAAGCTGTG TAATTAAGAA TCCAACGGGA 
GAACCAATCC CCCCACGGAC TTTGACAGAA GCTGGCACGA TGGCATTGTG TTATAGTGCT 
GCCTGGGATG CTCGAGTTAT CACCAGTGCT TGGTGGGTGT ATCATCATCA GGTCTCTAAA 
ACAGCACCAA CTGGAGAATA TTTGACAACA GGAAGCTTCA TGATAAGAGG AAAGAAGAAT 
TTTCTTCCTC CTTCATATCT TATGATGGGG TTTAGTTTCC TATTTAAGGT AGATGAACCA 
TGTGTTTGGA GACATCGAGG GGAACGGAAA GTCAGAGGTC AAGATGAAGA TTTGGAGACA 
GTGGCTAGCA GCACTAGTAA GCTAGTTTCA GAAGAAATGG AGCCATTAGA TAATGGTGAT 
AGCAGCAGTG GAGAGGATCA AGAAGAAACT TCTGAAACTG TAGAAGAAAG GGAAGTAGTA 
AATCAAATTG ATGAAGAGGT CATTAGCATT CAAAATGACA AAAACAGGCC AAAAGAAGGA 
TCAGCACAAG AAGAGAGCTC AGATGATGAT GCTGCGGATG ATGCTGATGC TGATGCTGAT 
GATGATGAGG AGGAGGAGGA GGACGATGAC GACGACGATG ATGATCATGA TCATGATAAC 
ACCAATGACG ATAGAAAATC TCAAAGAATG AAATCAGATC AGGAAATAGC AAGTAAAAGG 
AAGGATGAAT CTGAAATGTC ATTAAATTAT CCAGATACTA CAATTGATCT ATCTCACCTT 
CAGTCCCAAA GATCCTTCCA GAAAACAGTT ACTAGAGAAG ATGCTTCTGA TGTGAATGAC 
AGTAAATTAC ATGGCCGGAG GCATTTGTCC GCCAAGGAAC GAAGGGAAAT GAAGAAAAAA 
AAGCAACCAA ATGATTCAAC AGATTTAGAT ATATTGGAGG ACAAGGGAAA AGAAAATACA 
CTGAAGACTG AAGTTTTTCC AAATACAAGC AAAACTGTTT CGGGTCCACA ACCAATGAAG 
AGAGGACAGA AGAGTAAAAT TAAAAAAATG AAAGAAAAAT ATAAAGATCA AGATGAGGAA 
GATCGTGAAC TTATCATGAA GCTCCTTGGA TCTGCAGGTT CAAGCAAAGA AGAAAAAGGA 
AAGAAAGGGA AGAAAGGAAA AACGGGGAAA ACAAAAGAAG AGGCAACAAA GAAACAACCC 
CAGAAGTTCA GAAGTGAACT GAGAATTGGT GACAGAATTA AGAAAGAAAC TCCTCTTGAA 
GCTGTAATTC ATGAATTACA AGAAATTACC ATGGACGATC AGCCAGATGA TAAGCAGGAA 
GAACAAGATG TAGACCAACA AGGAAATGAG GAGAACCTGC TGGATTCTTT AACAGGACAA 
CCCCACTCTG AAGATGTTTT GCTTTTTGCT ATACCAGTAT GTGCCCCTTA TACCACCATG 
ACCAACTATA AATACAAAGT GAAACTTACT CCTGGAGTCC AAAAAAAAGG AAAAGCGGCT 
AAAACTGCCT TGAATACTTT CATGCATTCA AAAGAAGCAA CAGCAAGAGA AAAAGATTTA 
TTTCGCAGTG TAAAGGACAC TGATTTATCA AGAAATATTC CTGGAAAAGT GAAAGTATCT 
GCTCCCAATC TCCTTAATGC AAAGAAGAAA AATAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031808677.1
CDS	108..3446
Translation	MKTRFSSVDICAILSEFNARLLGMRVYNIYDVDNKTYLIRLQKPDFKVTLLLESGIRIHTTEFEWPKNMMPSSFAMKCRKHLKSRRLVSVKQLGVDRIVDFQFGSDEAAYHLIIELYDKGNIVLTNYEYLILNILRFRSDEADDVKFAVREKYPIDHARVMEPLLSLERLTEIISSAPKGEQLRRILNPYLPYGATLIEHCLRENGFSSYFRVDEKFETGDIEKVLVCLQKAEDHMKTMSNFSGKGYIIQKKEKKPSLEPDKQSEDILTYEEFHPFLFSQHSKCPYIEFESFDKAVDEFYSKLEGQKIDLKALQQEKQALKKLDNVRKDHEHRLEALHQAQEIDKIKGELIEMNLQIVDRAIQVVRSALANQIDWTEIGLIVKEAQAQGDIVANAIRELKLQTNHVTMLLKNPYLISDEEEEDDEINIEKEETEEPKGKKKKQKNKQLQKLQKNKPLLVDVDLSLSAYANAKKYYDHKRHAARKTQKTVEAAEKAFRSAEKKTKQTLKEVQMVTTIQKARKVYWFEKFLWFISSENYLIIGGRDQQQNEMIVKRYLTPGDIYVHADLHGATSCVIKNPTGEPIPPRTLTEAGTMALCYSAAWDARVITSAWWVYHHQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDEPCVWRHRGERKVRGQDEDLETVASSTSKLVSEEMEPLDNGDSSSGEDQEETSETVEEREVVNQIDEEVISIQNDKNRPKEGSAQEESSDDDAADDADADADDDEEEEEDDDDDDDDHDHDNTNDDRKSQRMKSDQEIASKRKDESEMSLNYPDTTIDLSHLQSQRSFQKTVTREDASDVNDSKLHGRRHLSAKERREMKKKKQPNDSTDLDILEDKGKENTLKTEVFPNTSKTVSGPQPMKRGQKSKIKKMKEKYKDQDEEDRELIMKLLGSAGSSKEEKGKKGKKGKTGKTKEEATKKQPQKFRSELRIGDRIKKETPLEAVIHELQEITMDDQPDDKQEEQDVDQQGNEENLLDSLTGQPHSEDVLLFAIPVCAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNTFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLNAKKKNN

Target ORF information:

RefSeq Version	XM_031952817.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii nuclear export mediator factor (NEMF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031952817.1

ORF Insert Sequence:

ATGAAGACGC GTTTCAGCTC CGTAGATATT TGCGCTATCC TCTCCGAGTT CAATGCCAGA 
TTATTGGGAA TGAGAGTATA CAATATTTAT GATGTGGATA ATAAGACATA CCTTATACGC 
CTTCAAAAGC CAGACTTTAA AGTTACGCTT CTACTTGAAT CTGGTATACG AATTCACACG 
ACAGAGTTTG AATGGCCAAA GAATATGATG CCATCTAGCT TTGCCATGAA GTGCCGAAAG 
CATTTGAAGA GTCGAAGACT GGTCAGTGTA AAACAGCTTG GTGTGGATCG GATTGTAGAT 
TTTCAGTTTG GAAGTGATGA AGCTGCCTAT CATTTAATCA TTGAGCTTTA TGATAAAGGC 
AATATTGTTC TTACCAACTA TGAATACCTC ATTTTAAATA TCTTGAGGTT TAGAAGTGAT 
GAAGCAGATG ATGTGAAGTT TGCTGTTCGA GAAAAATATC CAATTGATCA TGCTAGGGTA 
ATGGAACCAT TACTTTCACT GGAAAGATTA ACTGAAATAA TATCCAGTGC ACCAAAAGGA 
GAACAATTAA GGAGGATTCT TAATCCATAT CTCCCCTACG GGGCCACTCT GATTGAACAC 
TGTCTTAGAG AAAATGGATT TTCCAGCTAT TTCAGAGTAG ATGAGAAATT TGAAACTGGA 
GATATTGAAA AAGTACTTGT TTGTCTGCAA AAAGCAGAAG ATCATATGAA AACAATGTCC 
AACTTCAGTG GAAAGGGGTA TATCATTCAG AAAAAAGAGA AAAAACCAAG CCTGGAGCCA 
GATAAGCAAT CTGAAGATAT ACTCACATAT GAAGAATTTC ATCCTTTCTT ATTTTCTCAA 
CATTCCAAAT GTCCATATAT AGAATTTGAA TCATTTGACA AGGCAGTGGA TGAATTTTAT 
TCTAAGCTAG AAGGCCAGAA GATAGACTTG AAAGCTTTAC AACAGGAGAA ACAAGCATTG 
AAGAAATTGG ATAATGTCCG TAAGGATCAT GAGCACAGAT TAGAAGCCCT TCACCAAGCT 
CAGGAAATTG ATAAAATCAA AGGAGAACTC ATAGAAATGA ATCTACAGAT AGTTGATAGA 
GCCATCCAAG TAGTTCGGAG TGCTTTAGCC AACCAGATAG ACTGGACAGA AATTGGACTA 
ATTGTAAAAG AGGCCCAAGC TCAAGGAGAT ATTGTTGCAA ATGCAATCAG AGAATTGAAG 
CTGCAAACCA ATCATGTCAC AATGCTGCTA AAAAATCCAT ATCTAATATC AGATGAGGAG 
GAGGAAGATG ATGAAATTAA TATTGAAAAA GAAGAAACAG AAGAACCAAA AGGGAAAAAA 
AAAAAGCAGA AGAACAAACA ACTTCAGAAA CTTCAGAAGA ATAAGCCATT ACTTGTTGAT 
GTTGATCTCA GTTTGTCAGC ATATGCCAAT GCCAAAAAAT ATTATGACCA CAAGAGACAT 
GCTGCCAGAA AAACACAAAA AACTGTTGAA GCTGCTGAGA AGGCATTCAG ATCTGCGGAA 
AAGAAAACAA AACAAACCCT CAAAGAAGTT CAGATGGTTA CCACTATTCA GAAAGCAAGG 
AAAGTGTATT GGTTTGAGAA GTTTTTATGG TTTATTAGCT CTGAGAACTA TCTCATTATA 
GGTGGAAGAG ATCAACAACA AAATGAAATG ATTGTGAAGC GATATTTGAC ACCAGGAGAT 
ATTTATGTTC ATGCTGATCT TCATGGTGCA ACAAGCTGTG TAATTAAGAA TCCAACGGGA 
GAACCAATCC CCCCACGGAC TTTGACAGAA GCTGGCACGA TGGCATTGTG TTATAGTGCT 
GCCTGGGATG CTCGAGTTAT CACCAGTGCT TGGTGGGTGT ATCATCATCA GGTCTCTAAA 
ACAGCACCAA CTGGAGAATA TTTGACAACA GGAAGCTTCA TGATAAGAGG AAAGAAGAAT 
TTTCTTCCTC CTTCATATCT TATGATGGGG TTTAGTTTCC TATTTAAGGT AGATGAACCA 
TGTGTTTGGA GACATCGAGG GGAACGGAAA GTCAGAGGTC AAGATGAAGA TTTGGAGACA 
GTGGCTAGCA GCACTAGTAA GCTAGTTTCA GAAGAAATGG AGCCATTAGA TAATGGTGAT 
AGCAGCAGTG GAGAGGATCA AGAAGAAACT TCTGAAACTG TAGAAGAAAG GGAAGTAGTA 
AATCAAATTG ATGAAGAGGT CATTAGCATT CAAAATGACA AAAACAGGCC AAAAGAAGGA 
TCAGCACAAG AAGAGAGCTC AGATGATGAT GCTGCGGATG ATGCTGATGC TGATGCTGAT 
GATGATGAGG AGGAGGAGGA GGACGATGAC GACGACGATG ATGATCATGA TCATGATAAC 
ACCAATGACG ATAGAAAATC TCAAAGAATG AAATCAGATC AGGAAATAGC AAGTAAAAGG 
AAGGATGAAT CTGAAATGTC ATTAAATTAT CCAGATACTA CAATTGATCT ATCTCACCTT 
CAGTCCCAAA GATCCTTCCA GAAAACAGTT ACTAGAGAAG ATGCTTCTGA TGTGAATGAC 
AGTAAATTAC ATGGCCGGAG GCATTTGTCC GCCAAGGAAC GAAGGGAAAT GAAGAAAAAA 
AAGCAACCAA ATGATTCAAC AGATTTAGAT ATATTGGAGG ACAAGGGAAA AGAAAATACA 
CTGAAGACTG AAGTTTTTCC AAATACAAGC AAAACTGTTT CGGGTCCACA ACCAATGAAG 
AGAGGACAGA AGAGTAAAAT TAAAAAAATG AAAGAAAAAT ATAAAGATCA AGATGAGGAA 
GATCGTGAAC TTATCATGAA GCTCCTTGGA TCTGCAGGTT CAAGCAAAGA AGAAAAAGGA 
AAGAAAGGGA AGAAAGGAAA AACGGGGAAA ACAAAAGAAG AGGCAACAAA GAAACAACCC 
CAGAAGTTCA GAAGTGAACT GAGAATTGGT GACAGAATTA AGAAAGAAAC TCCTCTTGAA 
GCTGTAATTC ATGAATTACA AGAAATTACC ATGGACGATC AGCCAGATGA TAAGCAGGAA 
GAACAAGATG TAGACCAACA AGGAAATGAG GAGAACCTGC TGGATTCTTT AACAGGACAA 
CCCCACTCTG AAGATGTTTT GCTTTTTGCT ATACCAGTAT GTGCCCCTTA TACCACCATG 
ACCAACTATA AATACAAAGT GAAACTTACT CCTGGAGTCC AAAAAAAAGG AAAAGCGGCT 
AAAACTGCCT TGAATACTTT CATGCATTCA AAAGAAGCAA CAGCAAGAGA AAAAGATTTA 
TTTCGCAGTG TAAAGGACAC TGATTTATCA AGAAATATTC CTGGAAAAGT GAAAGTATCT 
GCTCCCAATC TCCTTAATGC AAAGAAGAAA AATAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEMF ( XM_031952818.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031808678.1 Sarcophilus harrisii nuclear export mediator factor (NEMF), transcript variant X3, mRNA.

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CloneID	OSa225340
Clone ID Related Accession (Same CDS sequence)	XM_031952818.1
Accession Version	XM_031952818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	nuclear export mediator factor NEMF isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGC GTTTCAGCTC CGTAGATATT TGCGCTATCC TCTCCGAGTT CAATGCCAGA 
TTATTGGGAA TGAGAGTATA CAATATTTAT GATGTGGATA ATAAGACATA CCTTATACGC 
CTTCAAAAGC CAGACTTTAA AGTTACGCTT CTACTTGAAT CTGGTATACG AATTCACACG 
ACAGAGTTTG AATGGCCAAA GAATATGATG CCATCTAGCT TTGCCATGAA GTGCCGAAAG 
CATTTGAAGA GTCGAAGACT GGTCAGTGTA AAACAGCTTG GTGTGGATCG GATTGTAGAT 
TTTCAGTTTG GAAGTGATGA AGCTGCCTAT CATTTAATCA TTGAGCTTTA TGATAAAGGC 
AATATTGTTC TTACCAACTA TGAATACCTC ATTTTAAATA TCTTGAGGTT TAGAAGTGAT 
GAAGCAGATG ATGTGAAGTT TGCTGTTCGA GAAAAATATC CAATTGATCA TGCTAGGGTA 
ATGGAACCAT TACTTTCACT GGAAAGATTA ACTGAAATAA TATCCAGTGC ACCAAAAGGA 
GAACAATTAA GGAGGATTCT TAATCCATAT CTCCCCTACG GGGCCACTCT GATTGAACAC 
TGTCTTAGAG AAAATGGATT TTCCAGCTAT TTCAGAGTAG ATGAGAAATT TGAAACTGGA 
GATATTGAAA AAGTACTTGT TTGTCTGCAA AAAGCAGAAG ATCATATGAA AACAATGTCC 
AACTTCAGTG GAAAGGGGTA TATCATTCAG AAAAAAGAGA AAAAACCAAG CCTGGAGCCA 
GATAAGCAAT CTGAAGATAT ACTCACATAT GAAGAATTTC ATCCTTTCTT ATTTTCTCAA 
CATTCCAAAT GTCCATATAT AGAATTTGAA TCATTTGACA AGGCAGTGGA TGAATTTTAT 
TCTAAGCTAG AAGGCCAGAA GATAGACTTG AAAGCTTTAC AACAGGAGAA ACAAGCATTG 
AAGAAATTGG ATAATGTCCG TAAGGATCAT GAGCACAGAT TAGAAGCCCT TCACCAAGCT 
CAGGAAATTG ATAAAATCAA AGGAGAACTC ATAGAAATGA ATCTACAGAT AGTTGATAGA 
GCCATCCAAG TAGTTCGGAG TGCTTTAGCC AACCAGATAG ACTGGACAGA AATTGGACTA 
ATTGTAAAAG AGGCCCAAGC TCAAGGAGAT ATTGTTGCAA ATGCAATCAG AGAATTGAAG 
CTGCAAACCA ATCATGTCAC AATGCTGCTA AAAAATCCAT ATCTAATATC AGATGAGGAG 
GAGGAAGATG ATGAAATTAA TATTGAAAAA GAAGAAACAG AAGAACCAAA AGGGAAAAAA 
AAAAAGCAGA AGAACAAACA ACTTCAGAAA CTTCAGAAGA ATAAGCCATT ACTTGTTGAT 
GTTGATCTCA GTTTGTCAGC ATATGCCAAT GCCAAAAAAT ATTATGACCA CAAGAGACAT 
GCTGCCAGAA AAACACAAAA AACTGTTGAA GCTGCTGAGA AGGCATTCAG ATCTGCGGAA 
AAGAAAACAA AACAAACCCT CAAAGAAGTT CAGATGGTTA CCACTATTCA GAAAGCAAGG 
AAAGTGTATT GGTTTGAGAA GTTTTTATGG TTTATTAGCT CTGAGAACTA TCTCATTATA 
GGTGGAAGAG ATCAACAACA AAATGAAATG ATTGTGAAGC GATATTTGAC ACCAGGAGAT 
ATTTATGTTC ATGCTGATCT TCATGGTGCA ACAAGCTGTG TAATTAAGAA TCCAACGGGA 
GAACCAATCC CCCCACGGAC TTTGACAGAA GCTGGCACGA TGGCATTGTG TTATAGTGCT 
GCCTGGGATG CTCGAGTTAT CACCAGTGCT TGGTGGGTGT ATCATCATCA GGTCTCTAAA 
ACAGCACCAA CTGGAGAATA TTTGACAACA GGAAGCTTCA TGATAAGAGG AAAGAAGAAT 
TTTCTTCCTC CTTCATATCT TATGATGGGG TTTAGTTTCC TATTTAAGGT AGATGAACCA 
TGTGTTTGGA GACATCGAGG GGAACGGAAA GTCAGAGGTC AAGATGAAGA TTTGGAGACA 
GTGGCTAGCA GCACTAGTAA GCTAGTTTCA GAAGAAATGG AGCCATTAGA TAATGGTGAT 
AGCAGCAGTG GAGAGGATCA AGAAGAAACT TCTGAAACTG TAGAAGAAAG GGAAGTAGTA 
AATCAAATTG ATGAAGAGGT CATTAGCATT CAAAATGACA AAAACAGGCC AAAAGAAGGA 
TCAGCACAAG AAGAGAGCTC AGATGATGAT GCTGCGGATG ATGCTGATGC TGATGCTGAT 
GATGATGAGG AGGAGGAGGA GGACGATGAC GACGACGATG ATGATCATGA TCATGATAAC 
ACCAATGACG ATAGAAAATC TCAAAGAATG AAATCAGATC AGGAAATAGC AAGTAAAAGG 
AAGGATGAAT CTGAAATGTC ATTAAATTAT CCAGATACTA CAATTGATCT ATCTCACCTT 
CAGTCCCAAA GATCCTTCCA GAAAACAGTT ACTAGAGAAG ATGCTTCTGA TGTGAATGAC 
AGTAAATTAC ATGGCCGGAG GCATTTGTCC GCCAAGGAAC GAAGGGAAAT GAAGAAAAAA 
AAGCAACCAA ATGATTCAAC AGATTTAGAT ATATTGGAGG ACAAGGGAAA AGAAAATACA 
CTGAAGACTG AAGTTTTTCC AAATACAAGC AAAACTGTTT CGGGTCCACA ACCAATGAAG 
AGAGGACAGA AGAGTAAAAT TAAAAAAATG AAAGAAAAAT ATAAAGATCA AGATGAGGAA 
GATCGTGAAC TTATCATGAA GCTCCTTGGA TCTGCAGGTT CAAGCAAAGA AGAAAAAGGA 
AAGAAAGGGA AGAAAGGAAA AACGGGGAAA ACAAAAGAAG AGGCAACAAA GAAACAACCC 
CAGAAGTTCA GAAGTGAACT GAGAATTGGT GACAGAATTA AGAAAGAAAC TCCTCTTGAA 
GCTGTAATTC ATGAATTACA AGAAATTACC ATGGACGATC AGCCAGATGA TAAGGAAGAA 
CAAGATGTAG ACCAACAAGG AAATGAGGAG AACCTGCTGG ATTCTTTAAC AGGACAACCC 
CACTCTGAAG ATGTTTTGCT TTTTGCTATA CCAGTATGTG CCCCTTATAC CACCATGACC 
AACTATAAAT ACAAAGTGAA ACTTACTCCT GGAGTCCAAA AAAAAGGAAA AGCGGCTAAA 
ACTGCCTTGA ATACTTTCAT GCATTCAAAA GAAGCAACAG CAAGAGAAAA AGATTTATTT 
CGCAGTGTAA AGGACACTGA TTTATCAAGA AATATTCCTG GAAAAGTGAA AGTATCTGCT 
CCCAATCTCC TTAATGCAAA GAAGAAAAAT AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031808678.1
CDS	107..3442
Translation	MKTRFSSVDICAILSEFNARLLGMRVYNIYDVDNKTYLIRLQKPDFKVTLLLESGIRIHTTEFEWPKNMMPSSFAMKCRKHLKSRRLVSVKQLGVDRIVDFQFGSDEAAYHLIIELYDKGNIVLTNYEYLILNILRFRSDEADDVKFAVREKYPIDHARVMEPLLSLERLTEIISSAPKGEQLRRILNPYLPYGATLIEHCLRENGFSSYFRVDEKFETGDIEKVLVCLQKAEDHMKTMSNFSGKGYIIQKKEKKPSLEPDKQSEDILTYEEFHPFLFSQHSKCPYIEFESFDKAVDEFYSKLEGQKIDLKALQQEKQALKKLDNVRKDHEHRLEALHQAQEIDKIKGELIEMNLQIVDRAIQVVRSALANQIDWTEIGLIVKEAQAQGDIVANAIRELKLQTNHVTMLLKNPYLISDEEEEDDEINIEKEETEEPKGKKKKQKNKQLQKLQKNKPLLVDVDLSLSAYANAKKYYDHKRHAARKTQKTVEAAEKAFRSAEKKTKQTLKEVQMVTTIQKARKVYWFEKFLWFISSENYLIIGGRDQQQNEMIVKRYLTPGDIYVHADLHGATSCVIKNPTGEPIPPRTLTEAGTMALCYSAAWDARVITSAWWVYHHQVSKTAPTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDEPCVWRHRGERKVRGQDEDLETVASSTSKLVSEEMEPLDNGDSSSGEDQEETSETVEEREVVNQIDEEVISIQNDKNRPKEGSAQEESSDDDAADDADADADDDEEEEEDDDDDDDDHDHDNTNDDRKSQRMKSDQEIASKRKDESEMSLNYPDTTIDLSHLQSQRSFQKTVTREDASDVNDSKLHGRRHLSAKERREMKKKKQPNDSTDLDILEDKGKENTLKTEVFPNTSKTVSGPQPMKRGQKSKIKKMKEKYKDQDEEDRELIMKLLGSAGSSKEEKGKKGKKGKTGKTKEEATKKQPQKFRSELRIGDRIKKETPLEAVIHELQEITMDDQPDDKEEQDVDQQGNEENLLDSLTGQPHSEDVLLFAIPVCAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNTFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLNAKKKNN

Target ORF information:

RefSeq Version	XM_031952818.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii nuclear export mediator factor (NEMF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031952818.1

ORF Insert Sequence:

ATGAAGACGC GTTTCAGCTC CGTAGATATT TGCGCTATCC TCTCCGAGTT CAATGCCAGA 
TTATTGGGAA TGAGAGTATA CAATATTTAT GATGTGGATA ATAAGACATA CCTTATACGC 
CTTCAAAAGC CAGACTTTAA AGTTACGCTT CTACTTGAAT CTGGTATACG AATTCACACG 
ACAGAGTTTG AATGGCCAAA GAATATGATG CCATCTAGCT TTGCCATGAA GTGCCGAAAG 
CATTTGAAGA GTCGAAGACT GGTCAGTGTA AAACAGCTTG GTGTGGATCG GATTGTAGAT 
TTTCAGTTTG GAAGTGATGA AGCTGCCTAT CATTTAATCA TTGAGCTTTA TGATAAAGGC 
AATATTGTTC TTACCAACTA TGAATACCTC ATTTTAAATA TCTTGAGGTT TAGAAGTGAT 
GAAGCAGATG ATGTGAAGTT TGCTGTTCGA GAAAAATATC CAATTGATCA TGCTAGGGTA 
ATGGAACCAT TACTTTCACT GGAAAGATTA ACTGAAATAA TATCCAGTGC ACCAAAAGGA 
GAACAATTAA GGAGGATTCT TAATCCATAT CTCCCCTACG GGGCCACTCT GATTGAACAC 
TGTCTTAGAG AAAATGGATT TTCCAGCTAT TTCAGAGTAG ATGAGAAATT TGAAACTGGA 
GATATTGAAA AAGTACTTGT TTGTCTGCAA AAAGCAGAAG ATCATATGAA AACAATGTCC 
AACTTCAGTG GAAAGGGGTA TATCATTCAG AAAAAAGAGA AAAAACCAAG CCTGGAGCCA 
GATAAGCAAT CTGAAGATAT ACTCACATAT GAAGAATTTC ATCCTTTCTT ATTTTCTCAA 
CATTCCAAAT GTCCATATAT AGAATTTGAA TCATTTGACA AGGCAGTGGA TGAATTTTAT 
TCTAAGCTAG AAGGCCAGAA GATAGACTTG AAAGCTTTAC AACAGGAGAA ACAAGCATTG 
AAGAAATTGG ATAATGTCCG TAAGGATCAT GAGCACAGAT TAGAAGCCCT TCACCAAGCT 
CAGGAAATTG ATAAAATCAA AGGAGAACTC ATAGAAATGA ATCTACAGAT AGTTGATAGA 
GCCATCCAAG TAGTTCGGAG TGCTTTAGCC AACCAGATAG ACTGGACAGA AATTGGACTA 
ATTGTAAAAG AGGCCCAAGC TCAAGGAGAT ATTGTTGCAA ATGCAATCAG AGAATTGAAG 
CTGCAAACCA ATCATGTCAC AATGCTGCTA AAAAATCCAT ATCTAATATC AGATGAGGAG 
GAGGAAGATG ATGAAATTAA TATTGAAAAA GAAGAAACAG AAGAACCAAA AGGGAAAAAA 
AAAAAGCAGA AGAACAAACA ACTTCAGAAA CTTCAGAAGA ATAAGCCATT ACTTGTTGAT 
GTTGATCTCA GTTTGTCAGC ATATGCCAAT GCCAAAAAAT ATTATGACCA CAAGAGACAT 
GCTGCCAGAA AAACACAAAA AACTGTTGAA GCTGCTGAGA AGGCATTCAG ATCTGCGGAA 
AAGAAAACAA AACAAACCCT CAAAGAAGTT CAGATGGTTA CCACTATTCA GAAAGCAAGG 
AAAGTGTATT GGTTTGAGAA GTTTTTATGG TTTATTAGCT CTGAGAACTA TCTCATTATA 
GGTGGAAGAG ATCAACAACA AAATGAAATG ATTGTGAAGC GATATTTGAC ACCAGGAGAT 
ATTTATGTTC ATGCTGATCT TCATGGTGCA ACAAGCTGTG TAATTAAGAA TCCAACGGGA 
GAACCAATCC CCCCACGGAC TTTGACAGAA GCTGGCACGA TGGCATTGTG TTATAGTGCT 
GCCTGGGATG CTCGAGTTAT CACCAGTGCT TGGTGGGTGT ATCATCATCA GGTCTCTAAA 
ACAGCACCAA CTGGAGAATA TTTGACAACA GGAAGCTTCA TGATAAGAGG AAAGAAGAAT 
TTTCTTCCTC CTTCATATCT TATGATGGGG TTTAGTTTCC TATTTAAGGT AGATGAACCA 
TGTGTTTGGA GACATCGAGG GGAACGGAAA GTCAGAGGTC AAGATGAAGA TTTGGAGACA 
GTGGCTAGCA GCACTAGTAA GCTAGTTTCA GAAGAAATGG AGCCATTAGA TAATGGTGAT 
AGCAGCAGTG GAGAGGATCA AGAAGAAACT TCTGAAACTG TAGAAGAAAG GGAAGTAGTA 
AATCAAATTG ATGAAGAGGT CATTAGCATT CAAAATGACA AAAACAGGCC AAAAGAAGGA 
TCAGCACAAG AAGAGAGCTC AGATGATGAT GCTGCGGATG ATGCTGATGC TGATGCTGAT 
GATGATGAGG AGGAGGAGGA GGACGATGAC GACGACGATG ATGATCATGA TCATGATAAC 
ACCAATGACG ATAGAAAATC TCAAAGAATG AAATCAGATC AGGAAATAGC AAGTAAAAGG 
AAGGATGAAT CTGAAATGTC ATTAAATTAT CCAGATACTA CAATTGATCT ATCTCACCTT 
CAGTCCCAAA GATCCTTCCA GAAAACAGTT ACTAGAGAAG ATGCTTCTGA TGTGAATGAC 
AGTAAATTAC ATGGCCGGAG GCATTTGTCC GCCAAGGAAC GAAGGGAAAT GAAGAAAAAA 
AAGCAACCAA ATGATTCAAC AGATTTAGAT ATATTGGAGG ACAAGGGAAA AGAAAATACA 
CTGAAGACTG AAGTTTTTCC AAATACAAGC AAAACTGTTT CGGGTCCACA ACCAATGAAG 
AGAGGACAGA AGAGTAAAAT TAAAAAAATG AAAGAAAAAT ATAAAGATCA AGATGAGGAA 
GATCGTGAAC TTATCATGAA GCTCCTTGGA TCTGCAGGTT CAAGCAAAGA AGAAAAAGGA 
AAGAAAGGGA AGAAAGGAAA AACGGGGAAA ACAAAAGAAG AGGCAACAAA GAAACAACCC 
CAGAAGTTCA GAAGTGAACT GAGAATTGGT GACAGAATTA AGAAAGAAAC TCCTCTTGAA 
GCTGTAATTC ATGAATTACA AGAAATTACC ATGGACGATC AGCCAGATGA TAAGGAAGAA 
CAAGATGTAG ACCAACAAGG AAATGAGGAG AACCTGCTGG ATTCTTTAAC AGGACAACCC 
CACTCTGAAG ATGTTTTGCT TTTTGCTATA CCAGTATGTG CCCCTTATAC CACCATGACC 
AACTATAAAT ACAAAGTGAA ACTTACTCCT GGAGTCCAAA AAAAAGGAAA AGCGGCTAAA 
ACTGCCTTGA ATACTTTCAT GCATTCAAAA GAAGCAACAG CAAGAGAAAA AGATTTATTT 
CGCAGTGTAA AGGACACTGA TTTATCAAGA AATATTCCTG GAAAAGTGAA AGTATCTGCT 
CCCAATCTCC TTAATGCAAA GAAGAAAAAT AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NEMF cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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