TIE1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIE1
Entrez Gene ID	100929593
Full Name	tyrosine kinase with immunoglobulin like and EGF like domains 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012550062.2	XP_012405516.1	tyrosine-protein kinase receptor Tie-1
XM_031969284.1	XP_031825144.1	tyrosine-protein kinase receptor Tie-1 isoform X3
XM_031969282.1	XP_031825142.1	tyrosine-protein kinase receptor Tie-1 isoform X1
XM_012550062.1	XP_012405516.1	tyrosine-protein kinase receptor Tie-1
XM_031969283.1	XP_031825143.1	tyrosine-protein kinase receptor Tie-1 isoform X2

Pathways from BioSystems
Gene Ontology

The following TIE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa132620	XM_012550062.2 Latest version!	Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa235827	XM_031969282.1 Latest version!	Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa235828	XM_031969283.1 Latest version!	Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa235829	XM_031969284.1 Latest version!	Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OSa132620	XM_012550062.1	Sarcophilus harrisii tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIE1 ( XM_012550062.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012405516.1 Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), mRNA.

Price & Availability↑

CloneID	OSa132620
Clone ID Related Accession (Same CDS sequence)	XM_012550062.2 , XM_012550062.1
Accession Version	XM_012550062.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	tyrosine-protein kinase receptor Tie-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003839047.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012550062.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGGCCAAG GCGGCCCCCT TCTCCTCCTC CTCTTCCTTC TCAGCTACCA AGTGGGGGCA 
GCCATGGACC TCACGCTGCT GGCAGACCTT CGCCTCACAG ACCCCCAACG CTTTTTTCTG 
ACCTGTGTAT CTGGAGAATC TGGGGCAGGC AGGGGCCCAG ATGTCTGGGA CCCTCCCCTG 
CTCCTGGAAA AGGATGACCG AATCGTTCGG GCCTTCCCAC CTCGCCAGCC CCTCCATTCT 
ACAAGAAATG GATCTCGCCA CATCACGCTT CGGGGCTTCT CCCAGCCCTC TGACCTGGTC 
GGCGTATTTT CCTGCATTGG TGGAACCGGG TCCCGGCGCA CACGTGTGGT CTATGTACAC 
AATAGTCCTG GGGCCCACCT GCTCCCAGAC AAGGTGACTC ACACCGTGAA TGTGGGTGAC 
ACAGCTGTGC TGTCCGCTCA GATCCGCACG GAAAAACAAG TAGATGTGAT CTGGAAGAGT 
AACGGCTCCT ACTTCCACAC CCTGGACTGG CGTGAAGCCC AGGATGGGCG ATTCAGGATA 
GCGCTCCCCA ACCTGAAGGC CTCGGATGGC GGCATCTACA GCGCCACCTA CCTGCAGGGC 
AACCCCCTGG CCAGCGCCTT CTTCAGGGTC ATTGTGCGAG CCTGCAGGGA GGGCCGGTTC 
GGGCCAGGCT GCCAGCAGCA GTGTCCGGGC ACGTCCGGCT GCCGCGGACT CACCTTCTGC 
CTCCCGGACC CCTATGGCTG CTCCTGTGGG GCTGGCTGGG GGGGCGACCA GTGCCAAGAA 
GCGGCCACAT CCACGAGCGT GTGCGCACAT ACCCGAGAAT CTCCACTGCC CCCAGACCGA 
AACCCAGAAA TCGTGAACAT GGCCTCAGAG TTGGACTTCA ACGTGGGAGC CATGCCCGTA 
GTCAGCTGTG CAGCCACAGG GAATCCGCCC CCGGTGCGGG GCAGCATGGA GCTACGAAGG 
CCCGATGGCA CCATCTTCAT GCCGTCCAAG GCCATCATGG AGGTGGACAA GACTACGTCC 
GAGTTCCAGG TGCCGCCCTT GAGCCCCACA GACAGTGGCC TGTGGGAGTG TCGAGTGTCT 
ACGTCCGGGG GTCAGGACAG CAGGCACTTC AAGGTCAACA TCAAAGTTCC CCCCGTGCCC 
CTGGCCCCTC CGAAGCTCCT GGCCCGCCTC AGCCGCCAAC TTGTGGTCTC CCCACTGGTG 
CCGTTCGCAG GGGACGGGCC CATCTCGTCA GTGCGCCTTC GCTACCGGCC TCAGGACAGC 
TCCGCGGCCT GGTCCACCAT TGTGGTGGAC CCCAGCGAAA ATGTGACCCT CATGAACCTC 
CGGCCTCAGA CGGGGTACAG CGTCCTGGTG CAGTTGAGTC GCCCAGGAGA GGGGGGCGAG 
GGAGCCTGGG GGTCACCCTC CATCATGGCC ACTGACTGCC TTGAGCCCAA GTTGCGACCC 
CAGCTGGAGG ACTGGCATGT GGAGACCCCG GACCGGATCC GAGTGACCTG GTCCTTGCCT 
GCCGTGCCCG GTCCCCTGGT GGGAGACGGT TTCCTCCTAC TCCTGCGGGA CCCGGAACGG 
GGCCACGAGC TGCGTAAGAA CGTCACCTCT GCCCAGGCAC GCTCCACTAC TCTGAGCGGC 
CTTGTGCCCG GCACCTTCTA CCTCCTGGAA GTGCGGCTCT ACCATTGCAC GCGCCTGGGC 
CCCGCTTCCC CGCCAACGCG CGTGCTCCTG CCCCACTCTG GCCCCCCAGC TCCACGGCTG 
CTCCAGGCTG AGGCCCTGTC AGACTCGGCC ATCCGGCTGA CCTGGCAACG TCCAGAGGAC 
GTCGGAGCCA TTGCCAAGTA CATCGTGGAG CTGCAGGTGG TCGGAGGGTC TGGGGAGCCC 
CTGTGGGTGG ACACAGAAGG AGCCGAGGAG AGCACCGTCA TCCACAGCCT CAATGCCAGC 
ACCTGCTACC ACTTCCGGGT TCGAGCCAGC GCTCAGGGCC CGGGAGCCTG GAGCGAAGTA 
GTAGAGGAAA CGACGCTGGG TAATGGCCTG GAGAGCGAGG GCCCAGCCCA GCAGAGCCGG 
ACAGCAAGCC CCAGCCTGGA CCAGCAGCTG GTGCTGGCCA TCGTGGGCTC CGTGTCGGCC 
ACGTGCCTCA CCATCCTGGC CGCGCTGCTG GCCCTGATCT GCATCCGCAA AAGCTGCCTC 
CATCGAAGAC GGACCTTCAC CTACCAGTCG GGCTCGGGAG AAGAGACGGT GCTTCAGTTC 
AGCTCCGGGA CGCTGACCCT GACCCGCCGT CCGAAGCCGC AGCTGGAGCC TTTGAGTTAC 
CCCGTGCTGG AGTGGGAAGA CATCACCTTT GAGGACCTCA TTGGGGAGGG CAACTTCGGC 
CAGGTCCTTA GAGCCATGAT CAAGAAGGAT GGGCTCCAGA TGAATGCGGC CGTCAAGATG 
CTCAAAGGTC CCAGCCCTGT TCCAGCCCTC AGGCCCCGGT GCCCCTCTCC CCCGCCCGGC 
CTGACCTGCC CGTCCCCCCC AGGATACCTG TACATCGCCA TAGAGTACGC CCCCTACGGG 
AACCTGCTGG ACTTCCTTCG GAAGAGCCGC GTGCTGGAGA CGGACCCGGC CTTCGCCCAC 
GAGCACGGCA CGGCCTCCAC GCTCACGTCC CAGCAGCTCC TGCGCTTCGC CAGCGACGTG 
TCCAAGGGCA TGCAGTACCT GAGCGAGAAG CAGTTCATCC ACAGGGATCT GGCCGCCCGG 
AACGTGCTCG TGGGGGAGAA TCTGGCCTCC AAGATTGCAG ACTTCGGCCT GTCCCGGGGG 
GAGGAGGTCT ACGTGAAGAA GACCATGGGC CGGCTCCCTG TGCGCTGGAT GGCCATCGAG 
TCTCTGAACT ACAGTGTCTA CACCACCAAG AGTGACGTTT GGTCCTTCGG GGTTTTGCTC 
TGGGAGATCG TGAGCCTCGG GGGCACGCCG TACTGTGGGA TGACGTGCGC TGAGCTCTAC 
GAGAAGCTGC CCCAGGGTTA CCGCATGGAG CAACCCCGAA ATTGTGATGA CGAAGTGTAT 
GAGCTGATGC GCCAGTGCTG GAGGGACCGT CCCTACGAAA GGCCGCCCTT TGCGCAGATC 
TCCGTGCAGC TGGGCCGGAT GTTAGAGGCC AGGAAGGCCT ATGTGAACAT GGCGCTGTTT 
GAAAACTTCA CCTACGCAGG TATTGATGCG ACTGCTGAGG AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012405516.1
CDS	446..3613
Translation	MGQGGPLLLLLFLLSYQVGAAMDLTLLADLRLTDPQRFFLTCVSGESGAGRGPDVWDPPLLLEKDDRIVRAFPPRQPLHSTRNGSRHITLRGFSQPSDLVGVFSCIGGTGSRRTRVVYVHNSPGAHLLPDKVTHTVNVGDTAVLSAQIRTEKQVDVIWKSNGSYFHTLDWREAQDGRFRIALPNLKASDGGIYSATYLQGNPLASAFFRVIVRACREGRFGPGCQQQCPGTSGCRGLTFCLPDPYGCSCGAGWGGDQCQEAATSTSVCAHTRESPLPPDRNPEIVNMASELDFNVGAMPVVSCAATGNPPPVRGSMELRRPDGTIFMPSKAIMEVDKTTSEFQVPPLSPTDSGLWECRVSTSGGQDSRHFKVNIKVPPVPLAPPKLLARLSRQLVVSPLVPFAGDGPISSVRLRYRPQDSSAAWSTIVVDPSENVTLMNLRPQTGYSVLVQLSRPGEGGEGAWGSPSIMATDCLEPKLRPQLEDWHVETPDRIRVTWSLPAVPGPLVGDGFLLLLRDPERGHELRKNVTSAQARSTTLSGLVPGTFYLLEVRLYHCTRLGPASPPTRVLLPHSGPPAPRLLQAEALSDSAIRLTWQRPEDVGAIAKYIVELQVVGGSGEPLWVDTEGAEESTVIHSLNASTCYHFRVRASAQGPGAWSEVVEETTLGNGLESEGPAQQSRTASPSLDQQLVLAIVGSVSATCLTILAALLALICIRKSCLHRRRTFTYQSGSGEETVLQFSSGTLTLTRRPKPQLEPLSYPVLEWEDITFEDLIGEGNFGQVLRAMIKKDGLQMNAAVKMLKGPSPVPALRPRCPSPPPGLTCPSPPGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAHEHGTASTLTSQQLLRFASDVSKGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQISVQLGRMLEARKAYVNMALFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_012550062.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012550062.2

ORF Insert Sequence:

ATGGGCCAAG GCGGCCCCCT TCTCCTCCTC CTCTTCCTTC TCAGCTACCA AGTGGGGGCA 
GCCATGGACC TCACGCTGCT GGCAGACCTT CGCCTCACAG ACCCCCAACG CTTTTTTCTG 
ACCTGTGTAT CTGGAGAATC TGGGGCAGGC AGGGGCCCAG ATGTCTGGGA CCCTCCCCTG 
CTCCTGGAAA AGGATGACCG AATCGTTCGG GCCTTCCCAC CTCGCCAGCC CCTCCATTCT 
ACAAGAAATG GATCTCGCCA CATCACGCTT CGGGGCTTCT CCCAGCCCTC TGACCTGGTC 
GGCGTATTTT CCTGCATTGG TGGAACCGGG TCCCGGCGCA CACGTGTGGT CTATGTACAC 
AATAGTCCTG GGGCCCACCT GCTCCCAGAC AAGGTGACTC ACACCGTGAA TGTGGGTGAC 
ACAGCTGTGC TGTCCGCTCA GATCCGCACG GAAAAACAAG TAGATGTGAT CTGGAAGAGT 
AACGGCTCCT ACTTCCACAC CCTGGACTGG CGTGAAGCCC AGGATGGGCG ATTCAGGATA 
GCGCTCCCCA ACCTGAAGGC CTCGGATGGC GGCATCTACA GCGCCACCTA CCTGCAGGGC 
AACCCCCTGG CCAGCGCCTT CTTCAGGGTC ATTGTGCGAG CCTGCAGGGA GGGCCGGTTC 
GGGCCAGGCT GCCAGCAGCA GTGTCCGGGC ACGTCCGGCT GCCGCGGACT CACCTTCTGC 
CTCCCGGACC CCTATGGCTG CTCCTGTGGG GCTGGCTGGG GGGGCGACCA GTGCCAAGAA 
GCGGCCACAT CCACGAGCGT GTGCGCACAT ACCCGAGAAT CTCCACTGCC CCCAGACCGA 
AACCCAGAAA TCGTGAACAT GGCCTCAGAG TTGGACTTCA ACGTGGGAGC CATGCCCGTA 
GTCAGCTGTG CAGCCACAGG GAATCCGCCC CCGGTGCGGG GCAGCATGGA GCTACGAAGG 
CCCGATGGCA CCATCTTCAT GCCGTCCAAG GCCATCATGG AGGTGGACAA GACTACGTCC 
GAGTTCCAGG TGCCGCCCTT GAGCCCCACA GACAGTGGCC TGTGGGAGTG TCGAGTGTCT 
ACGTCCGGGG GTCAGGACAG CAGGCACTTC AAGGTCAACA TCAAAGTTCC CCCCGTGCCC 
CTGGCCCCTC CGAAGCTCCT GGCCCGCCTC AGCCGCCAAC TTGTGGTCTC CCCACTGGTG 
CCGTTCGCAG GGGACGGGCC CATCTCGTCA GTGCGCCTTC GCTACCGGCC TCAGGACAGC 
TCCGCGGCCT GGTCCACCAT TGTGGTGGAC CCCAGCGAAA ATGTGACCCT CATGAACCTC 
CGGCCTCAGA CGGGGTACAG CGTCCTGGTG CAGTTGAGTC GCCCAGGAGA GGGGGGCGAG 
GGAGCCTGGG GGTCACCCTC CATCATGGCC ACTGACTGCC TTGAGCCCAA GTTGCGACCC 
CAGCTGGAGG ACTGGCATGT GGAGACCCCG GACCGGATCC GAGTGACCTG GTCCTTGCCT 
GCCGTGCCCG GTCCCCTGGT GGGAGACGGT TTCCTCCTAC TCCTGCGGGA CCCGGAACGG 
GGCCACGAGC TGCGTAAGAA CGTCACCTCT GCCCAGGCAC GCTCCACTAC TCTGAGCGGC 
CTTGTGCCCG GCACCTTCTA CCTCCTGGAA GTGCGGCTCT ACCATTGCAC GCGCCTGGGC 
CCCGCTTCCC CGCCAACGCG CGTGCTCCTG CCCCACTCTG GCCCCCCAGC TCCACGGCTG 
CTCCAGGCTG AGGCCCTGTC AGACTCGGCC ATCCGGCTGA CCTGGCAACG TCCAGAGGAC 
GTCGGAGCCA TTGCCAAGTA CATCGTGGAG CTGCAGGTGG TCGGAGGGTC TGGGGAGCCC 
CTGTGGGTGG ACACAGAAGG AGCCGAGGAG AGCACCGTCA TCCACAGCCT CAATGCCAGC 
ACCTGCTACC ACTTCCGGGT TCGAGCCAGC GCTCAGGGCC CGGGAGCCTG GAGCGAAGTA 
GTAGAGGAAA CGACGCTGGG TAATGGCCTG GAGAGCGAGG GCCCAGCCCA GCAGAGCCGG 
ACAGCAAGCC CCAGCCTGGA CCAGCAGCTG GTGCTGGCCA TCGTGGGCTC CGTGTCGGCC 
ACGTGCCTCA CCATCCTGGC CGCGCTGCTG GCCCTGATCT GCATCCGCAA AAGCTGCCTC 
CATCGAAGAC GGACCTTCAC CTACCAGTCG GGCTCGGGAG AAGAGACGGT GCTTCAGTTC 
AGCTCCGGGA CGCTGACCCT GACCCGCCGT CCGAAGCCGC AGCTGGAGCC TTTGAGTTAC 
CCCGTGCTGG AGTGGGAAGA CATCACCTTT GAGGACCTCA TTGGGGAGGG CAACTTCGGC 
CAGGTCCTTA GAGCCATGAT CAAGAAGGAT GGGCTCCAGA TGAATGCGGC CGTCAAGATG 
CTCAAAGGTC CCAGCCCTGT TCCAGCCCTC AGGCCCCGGT GCCCCTCTCC CCCGCCCGGC 
CTGACCTGCC CGTCCCCCCC AGGATACCTG TACATCGCCA TAGAGTACGC CCCCTACGGG 
AACCTGCTGG ACTTCCTTCG GAAGAGCCGC GTGCTGGAGA CGGACCCGGC CTTCGCCCAC 
GAGCACGGCA CGGCCTCCAC GCTCACGTCC CAGCAGCTCC TGCGCTTCGC CAGCGACGTG 
TCCAAGGGCA TGCAGTACCT GAGCGAGAAG CAGTTCATCC ACAGGGATCT GGCCGCCCGG 
AACGTGCTCG TGGGGGAGAA TCTGGCCTCC AAGATTGCAG ACTTCGGCCT GTCCCGGGGG 
GAGGAGGTCT ACGTGAAGAA GACCATGGGC CGGCTCCCTG TGCGCTGGAT GGCCATCGAG 
TCTCTGAACT ACAGTGTCTA CACCACCAAG AGTGACGTTT GGTCCTTCGG GGTTTTGCTC 
TGGGAGATCG TGAGCCTCGG GGGCACGCCG TACTGTGGGA TGACGTGCGC TGAGCTCTAC 
GAGAAGCTGC CCCAGGGTTA CCGCATGGAG CAACCCCGAA ATTGTGATGA CGAAGTGTAT 
GAGCTGATGC GCCAGTGCTG GAGGGACCGT CCCTACGAAA GGCCGCCCTT TGCGCAGATC 
TCCGTGCAGC TGGGCCGGAT GTTAGAGGCC AGGAAGGCCT ATGTGAACAT GGCGCTGTTT 
GAAAACTTCA CCTACGCAGG TATTGATGCG ACTGCTGAGG AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_012550062.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012405516.1 Sarcophilus harrisii tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), mRNA.

Price & Availability↑

CloneID	OSa132620
Clone ID Related Accession (Same CDS sequence)	XM_012550062.2 , XM_012550062.1
Accession Version	XM_012550062.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	tyrosine-protein kinase receptor Tie-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003839047.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGGCCAAG GCGGCCCCCT TCTCCTCCTC CTCTTCCTTC TCAGCTACCA AGTGGGGGCA 
GCCATGGACC TCACGCTGCT GGCAGACCTT CGCCTCACAG ACCCCCAACG CTTTTTTCTG 
ACCTGTGTAT CTGGAGAATC TGGGGCAGGC AGGGGCCCAG ATGTCTGGGA CCCTCCCCTG 
CTCCTGGAAA AGGATGACCG AATCGTTCGG GCCTTCCCAC CTCGCCAGCC CCTCCATTCT 
ACAAGAAATG GATCTCGCCA CATCACGCTT CGGGGCTTCT CCCAGCCCTC TGACCTGGTC 
GGCGTATTTT CCTGCATTGG TGGAACCGGG TCCCGGCGCA CACGTGTGGT CTATGTACAC 
AATAGTCCTG GGGCCCACCT GCTCCCAGAC AAGGTGACTC ACACCGTGAA TGTGGGTGAC 
ACAGCTGTGC TGTCCGCTCA GATCCGCACG GAAAAACAAG TAGATGTGAT CTGGAAGAGT 
AACGGCTCCT ACTTCCACAC CCTGGACTGG CGTGAAGCCC AGGATGGGCG ATTCAGGATA 
GCGCTCCCCA ACCTGAAGGC CTCGGATGGC GGCATCTACA GCGCCACCTA CCTGCAGGGC 
AACCCCCTGG CCAGCGCCTT CTTCAGGGTC ATTGTGCGAG CCTGCAGGGA GGGCCGGTTC 
GGGCCAGGCT GCCAGCAGCA GTGTCCGGGC ACGTCCGGCT GCCGCGGACT CACCTTCTGC 
CTCCCGGACC CCTATGGCTG CTCCTGTGGG GCTGGCTGGG GGGGCGACCA GTGCCAAGAA 
GCGGCCACAT CCACGAGCGT GTGCGCACAT ACCCGAGAAT CTCCACTGCC CCCAGACCGA 
AACCCAGAAA TCGTGAACAT GGCCTCAGAG TTGGACTTCA ACGTGGGAGC CATGCCCGTA 
GTCAGCTGTG CAGCCACAGG GAATCCGCCC CCGGTGCGGG GCAGCATGGA GCTACGAAGG 
CCCGATGGCA CCATCTTCAT GCCGTCCAAG GCCATCATGG AGGTGGACAA GACTACGTCC 
GAGTTCCAGG TGCCGCCCTT GAGCCCCACA GACAGTGGCC TGTGGGAGTG TCGAGTGTCT 
ACGTCCGGGG GTCAGGACAG CAGGCACTTC AAGGTCAACA TCAAAGTTCC CCCCGTGCCC 
CTGGCCCCTC CGAAGCTCCT GGCCCGCCTC AGCCGCCAAC TTGTGGTCTC CCCACTGGTG 
CCGTTCGCAG GGGACGGGCC CATCTCGTCA GTGCGCCTTC GCTACCGGCC TCAGGACAGC 
TCCGCGGCCT GGTCCACCAT TGTGGTGGAC CCCAGCGAAA ATGTGACCCT CATGAACCTC 
CGGCCTCAGA CGGGGTACAG CGTCCTGGTG CAGTTGAGTC GCCCAGGAGA GGGGGGCGAG 
GGAGCCTGGG GGTCACCCTC CATCATGGCC ACTGACTGCC TTGAGCCCAA GTTGCGACCC 
CAGCTGGAGG ACTGGCATGT GGAGACCCCG GACCGGATCC GAGTGACCTG GTCCTTGCCT 
GCCGTGCCCG GTCCCCTGGT GGGAGACGGT TTCCTCCTAC TCCTGCGGGA CCCGGAACGG 
GGCCACGAGC TGCGTAAGAA CGTCACCTCT GCCCAGGCAC GCTCCACTAC TCTGAGCGGC 
CTTGTGCCCG GCACCTTCTA CCTCCTGGAA GTGCGGCTCT ACCATTGCAC GCGCCTGGGC 
CCCGCTTCCC CGCCAACGCG CGTGCTCCTG CCCCACTCTG GCCCCCCAGC TCCACGGCTG 
CTCCAGGCTG AGGCCCTGTC AGACTCGGCC ATCCGGCTGA CCTGGCAACG TCCAGAGGAC 
GTCGGAGCCA TTGCCAAGTA CATCGTGGAG CTGCAGGTGG TCGGAGGGTC TGGGGAGCCC 
CTGTGGGTGG ACACAGAAGG AGCCGAGGAG AGCACCGTCA TCCACAGCCT CAATGCCAGC 
ACCTGCTACC ACTTCCGGGT TCGAGCCAGC GCTCAGGGCC CGGGAGCCTG GAGCGAAGTA 
GTAGAGGAAA CGACGCTGGG TAATGGCCTG GAGAGCGAGG GCCCAGCCCA GCAGAGCCGG 
ACAGCAAGCC CCAGCCTGGA CCAGCAGCTG GTGCTGGCCA TCGTGGGCTC CGTGTCGGCC 
ACGTGCCTCA CCATCCTGGC CGCGCTGCTG GCCCTGATCT GCATCCGCAA AAGCTGCCTC 
CATCGAAGAC GGACCTTCAC CTACCAGTCG GGCTCGGGAG AAGAGACGGT GCTTCAGTTC 
AGCTCCGGGA CGCTGACCCT GACCCGCCGT CCGAAGCCGC AGCTGGAGCC TTTGAGTTAC 
CCCGTGCTGG AGTGGGAAGA CATCACCTTT GAGGACCTCA TTGGGGAGGG CAACTTCGGC 
CAGGTCCTTA GAGCCATGAT CAAGAAGGAT GGGCTCCAGA TGAATGCGGC CGTCAAGATG 
CTCAAAGGTC CCAGCCCTGT TCCAGCCCTC AGGCCCCGGT GCCCCTCTCC CCCGCCCGGC 
CTGACCTGCC CGTCCCCCCC AGGATACCTG TACATCGCCA TAGAGTACGC CCCCTACGGG 
AACCTGCTGG ACTTCCTTCG GAAGAGCCGC GTGCTGGAGA CGGACCCGGC CTTCGCCCAC 
GAGCACGGCA CGGCCTCCAC GCTCACGTCC CAGCAGCTCC TGCGCTTCGC CAGCGACGTG 
TCCAAGGGCA TGCAGTACCT GAGCGAGAAG CAGTTCATCC ACAGGGATCT GGCCGCCCGG 
AACGTGCTCG TGGGGGAGAA TCTGGCCTCC AAGATTGCAG ACTTCGGCCT GTCCCGGGGG 
GAGGAGGTCT ACGTGAAGAA GACCATGGGC CGGCTCCCTG TGCGCTGGAT GGCCATCGAG 
TCTCTGAACT ACAGTGTCTA CACCACCAAG AGTGACGTTT GGTCCTTCGG GGTTTTGCTC 
TGGGAGATCG TGAGCCTCGG GGGCACGCCG TACTGTGGGA TGACGTGCGC TGAGCTCTAC 
GAGAAGCTGC CCCAGGGTTA CCGCATGGAG CAACCCCGAA ATTGTGATGA CGAAGTGTAT 
GAGCTGATGC GCCAGTGCTG GAGGGACCGT CCCTACGAAA GGCCGCCCTT TGCGCAGATC 
TCCGTGCAGC TGGGCCGGAT GTTAGAGGCC AGGAAGGCCT ATGTGAACAT GGCGCTGTTT 
GAAAACTTCA CCTACGCAGG TATTGATGCG ACTGCTGAGG AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012405516.1
CDS	1..3168
Translation	MGQGGPLLLLLFLLSYQVGAAMDLTLLADLRLTDPQRFFLTCVSGESGAGRGPDVWDPPLLLEKDDRIVRAFPPRQPLHSTRNGSRHITLRGFSQPSDLVGVFSCIGGTGSRRTRVVYVHNSPGAHLLPDKVTHTVNVGDTAVLSAQIRTEKQVDVIWKSNGSYFHTLDWREAQDGRFRIALPNLKASDGGIYSATYLQGNPLASAFFRVIVRACREGRFGPGCQQQCPGTSGCRGLTFCLPDPYGCSCGAGWGGDQCQEAATSTSVCAHTRESPLPPDRNPEIVNMASELDFNVGAMPVVSCAATGNPPPVRGSMELRRPDGTIFMPSKAIMEVDKTTSEFQVPPLSPTDSGLWECRVSTSGGQDSRHFKVNIKVPPVPLAPPKLLARLSRQLVVSPLVPFAGDGPISSVRLRYRPQDSSAAWSTIVVDPSENVTLMNLRPQTGYSVLVQLSRPGEGGEGAWGSPSIMATDCLEPKLRPQLEDWHVETPDRIRVTWSLPAVPGPLVGDGFLLLLRDPERGHELRKNVTSAQARSTTLSGLVPGTFYLLEVRLYHCTRLGPASPPTRVLLPHSGPPAPRLLQAEALSDSAIRLTWQRPEDVGAIAKYIVELQVVGGSGEPLWVDTEGAEESTVIHSLNASTCYHFRVRASAQGPGAWSEVVEETTLGNGLESEGPAQQSRTASPSLDQQLVLAIVGSVSATCLTILAALLALICIRKSCLHRRRTFTYQSGSGEETVLQFSSGTLTLTRRPKPQLEPLSYPVLEWEDITFEDLIGEGNFGQVLRAMIKKDGLQMNAAVKMLKGPSPVPALRPRCPSPPPGLTCPSPPGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAHEHGTASTLTSQQLLRFASDVSKGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQISVQLGRMLEARKAYVNMALFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_012550062.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012550062.1

ORF Insert Sequence:

ATGGGCCAAG GCGGCCCCCT TCTCCTCCTC CTCTTCCTTC TCAGCTACCA AGTGGGGGCA 
GCCATGGACC TCACGCTGCT GGCAGACCTT CGCCTCACAG ACCCCCAACG CTTTTTTCTG 
ACCTGTGTAT CTGGAGAATC TGGGGCAGGC AGGGGCCCAG ATGTCTGGGA CCCTCCCCTG 
CTCCTGGAAA AGGATGACCG AATCGTTCGG GCCTTCCCAC CTCGCCAGCC CCTCCATTCT 
ACAAGAAATG GATCTCGCCA CATCACGCTT CGGGGCTTCT CCCAGCCCTC TGACCTGGTC 
GGCGTATTTT CCTGCATTGG TGGAACCGGG TCCCGGCGCA CACGTGTGGT CTATGTACAC 
AATAGTCCTG GGGCCCACCT GCTCCCAGAC AAGGTGACTC ACACCGTGAA TGTGGGTGAC 
ACAGCTGTGC TGTCCGCTCA GATCCGCACG GAAAAACAAG TAGATGTGAT CTGGAAGAGT 
AACGGCTCCT ACTTCCACAC CCTGGACTGG CGTGAAGCCC AGGATGGGCG ATTCAGGATA 
GCGCTCCCCA ACCTGAAGGC CTCGGATGGC GGCATCTACA GCGCCACCTA CCTGCAGGGC 
AACCCCCTGG CCAGCGCCTT CTTCAGGGTC ATTGTGCGAG CCTGCAGGGA GGGCCGGTTC 
GGGCCAGGCT GCCAGCAGCA GTGTCCGGGC ACGTCCGGCT GCCGCGGACT CACCTTCTGC 
CTCCCGGACC CCTATGGCTG CTCCTGTGGG GCTGGCTGGG GGGGCGACCA GTGCCAAGAA 
GCGGCCACAT CCACGAGCGT GTGCGCACAT ACCCGAGAAT CTCCACTGCC CCCAGACCGA 
AACCCAGAAA TCGTGAACAT GGCCTCAGAG TTGGACTTCA ACGTGGGAGC CATGCCCGTA 
GTCAGCTGTG CAGCCACAGG GAATCCGCCC CCGGTGCGGG GCAGCATGGA GCTACGAAGG 
CCCGATGGCA CCATCTTCAT GCCGTCCAAG GCCATCATGG AGGTGGACAA GACTACGTCC 
GAGTTCCAGG TGCCGCCCTT GAGCCCCACA GACAGTGGCC TGTGGGAGTG TCGAGTGTCT 
ACGTCCGGGG GTCAGGACAG CAGGCACTTC AAGGTCAACA TCAAAGTTCC CCCCGTGCCC 
CTGGCCCCTC CGAAGCTCCT GGCCCGCCTC AGCCGCCAAC TTGTGGTCTC CCCACTGGTG 
CCGTTCGCAG GGGACGGGCC CATCTCGTCA GTGCGCCTTC GCTACCGGCC TCAGGACAGC 
TCCGCGGCCT GGTCCACCAT TGTGGTGGAC CCCAGCGAAA ATGTGACCCT CATGAACCTC 
CGGCCTCAGA CGGGGTACAG CGTCCTGGTG CAGTTGAGTC GCCCAGGAGA GGGGGGCGAG 
GGAGCCTGGG GGTCACCCTC CATCATGGCC ACTGACTGCC TTGAGCCCAA GTTGCGACCC 
CAGCTGGAGG ACTGGCATGT GGAGACCCCG GACCGGATCC GAGTGACCTG GTCCTTGCCT 
GCCGTGCCCG GTCCCCTGGT GGGAGACGGT TTCCTCCTAC TCCTGCGGGA CCCGGAACGG 
GGCCACGAGC TGCGTAAGAA CGTCACCTCT GCCCAGGCAC GCTCCACTAC TCTGAGCGGC 
CTTGTGCCCG GCACCTTCTA CCTCCTGGAA GTGCGGCTCT ACCATTGCAC GCGCCTGGGC 
CCCGCTTCCC CGCCAACGCG CGTGCTCCTG CCCCACTCTG GCCCCCCAGC TCCACGGCTG 
CTCCAGGCTG AGGCCCTGTC AGACTCGGCC ATCCGGCTGA CCTGGCAACG TCCAGAGGAC 
GTCGGAGCCA TTGCCAAGTA CATCGTGGAG CTGCAGGTGG TCGGAGGGTC TGGGGAGCCC 
CTGTGGGTGG ACACAGAAGG AGCCGAGGAG AGCACCGTCA TCCACAGCCT CAATGCCAGC 
ACCTGCTACC ACTTCCGGGT TCGAGCCAGC GCTCAGGGCC CGGGAGCCTG GAGCGAAGTA 
GTAGAGGAAA CGACGCTGGG TAATGGCCTG GAGAGCGAGG GCCCAGCCCA GCAGAGCCGG 
ACAGCAAGCC CCAGCCTGGA CCAGCAGCTG GTGCTGGCCA TCGTGGGCTC CGTGTCGGCC 
ACGTGCCTCA CCATCCTGGC CGCGCTGCTG GCCCTGATCT GCATCCGCAA AAGCTGCCTC 
CATCGAAGAC GGACCTTCAC CTACCAGTCG GGCTCGGGAG AAGAGACGGT GCTTCAGTTC 
AGCTCCGGGA CGCTGACCCT GACCCGCCGT CCGAAGCCGC AGCTGGAGCC TTTGAGTTAC 
CCCGTGCTGG AGTGGGAAGA CATCACCTTT GAGGACCTCA TTGGGGAGGG CAACTTCGGC 
CAGGTCCTTA GAGCCATGAT CAAGAAGGAT GGGCTCCAGA TGAATGCGGC CGTCAAGATG 
CTCAAAGGTC CCAGCCCTGT TCCAGCCCTC AGGCCCCGGT GCCCCTCTCC CCCGCCCGGC 
CTGACCTGCC CGTCCCCCCC AGGATACCTG TACATCGCCA TAGAGTACGC CCCCTACGGG 
AACCTGCTGG ACTTCCTTCG GAAGAGCCGC GTGCTGGAGA CGGACCCGGC CTTCGCCCAC 
GAGCACGGCA CGGCCTCCAC GCTCACGTCC CAGCAGCTCC TGCGCTTCGC CAGCGACGTG 
TCCAAGGGCA TGCAGTACCT GAGCGAGAAG CAGTTCATCC ACAGGGATCT GGCCGCCCGG 
AACGTGCTCG TGGGGGAGAA TCTGGCCTCC AAGATTGCAG ACTTCGGCCT GTCCCGGGGG 
GAGGAGGTCT ACGTGAAGAA GACCATGGGC CGGCTCCCTG TGCGCTGGAT GGCCATCGAG 
TCTCTGAACT ACAGTGTCTA CACCACCAAG AGTGACGTTT GGTCCTTCGG GGTTTTGCTC 
TGGGAGATCG TGAGCCTCGG GGGCACGCCG TACTGTGGGA TGACGTGCGC TGAGCTCTAC 
GAGAAGCTGC CCCAGGGTTA CCGCATGGAG CAACCCCGAA ATTGTGATGA CGAAGTGTAT 
GAGCTGATGC GCCAGTGCTG GAGGGACCGT CCCTACGAAA GGCCGCCCTT TGCGCAGATC 
TCCGTGCAGC TGGGCCGGAT GTTAGAGGCC AGGAAGGCCT ATGTGAACAT GGCGCTGTTT 
GAAAACTTCA CCTACGCAGG TATTGATGCG ACTGCTGAGG AGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_031969282.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031825142.1 Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa235827
Clone ID Related Accession (Same CDS sequence)	XM_031969282.1
Accession Version	XM_031969282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	tyrosine-protein kinase receptor Tie-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAAG GCGGCCCCCT TCTCCTCCTC CTCTTCCTTC TCAGCTACCA AGTGGGGGCA 
GCCATGGACC TCACGCTGCT GGCAGACCTT CGCCTCACAG ACCCCCAACG CTTTTTTCTG 
ACCTGTGTAT CTGGAGAATC TGGGGCAGGC AGGGGCCCAG ATGTCTGGGA CCCTCCCCTG 
CTCCTGGAAA AGGATGACCG AATCGTTCGG GCCTTCCCAC CTCGCCAGCC CCTCCATTCT 
ACAAGAAATG GATCTCGCCA CATCACGCTT CGGGGCTTCT CCCAGCCCTC TGACCTGGTC 
GGCGTATTTT CCTGCATTGG TGGAACCGGG TCCCGGCGCA CACGTGTGGT CTATGTACAC 
AATAGTCCTG GGGCCCACCT GCTCCCAGAC AAGGTGACTC ACACCGTGAA TGTGGGTGAC 
ACAGCTGTGC TGTCCGCTCA GATCCGCACG GAAAAACAAG TAGATGTGAT CTGGAAGAGT 
AACGGCTCCT ACTTCCACAC CCTGGACTGG CGTGAAGCCC AGGATGGGCG ATTCAGGATA 
GCGCTCCCCA ACCTGAAGGC CTCGGATGGC GGCATCTACA GCGCCACCTA CCTGCAGGGC 
AACCCCCTGG CCAGCGCCTT CTTCAGGGTC ATTGTGCGAG GCTGCGGTGC GGGGCGATGG 
GGGCCGGGCT GCGCCAAGGA CTGCCCCAGC TGCCTGCATG GAGGCATTTG CCATGACCAA 
AATGGCGAGT GTGTGTGCCC CCCTGGCTTC ACGGGCACCC GCTGCGAGCA GGCCTGCAGG 
GAGGGCCGGT TCGGGCAAGG CTGCCAGCAG CAGTGTCCGG GCACGTCCGG CTGCCGCGGA 
CTCACCTTCT GCCTCCCGGA CCCCTATGGC TGCTCCTGTG GGGCTGGCTG GGGGGGCGAC 
CAGTGCCAAG AAGCCTGTTC CCCTGGCTGG TTTGGGGCCT CCTGTTACCT GCAGTGTCGA 
TGCCAGAACG GAGGCACCTG TGACCGCTTT AGTGGCTGTG TCTGTCCCTC AGGATGGCAC 
GGGGAGCACT GCGAAAAATC AGACCGAAAC CCAGAAATCG TGAACATGGC CTCAGAGTTG 
GACTTCAACG TGGGAGCCAT GCCCGTAGTC AGCTGTGCAG CCACAGGGAA TCCGCCCCCG 
GTGCGGGGCA GCATGGAGCT ACGAAGGCCC GATGGCACCA TCTTCATGCC GTCCAAGGCC 
ATCATGGAGG TGGACAAGAC TACGTCCGAG TTCCAGGTGC CGCCCTTGAG CCCCACAGAC 
AGTGGCCTGT GGGAGTGTCG AGTGTCTACG TCCGGGGGTC AGGACAGCAG GCACTTCAAG 
GTCAACATCA AAGTTCCCCC CGTGCCCCTG GCCCCTCCGA AGCTCCTGGC CCGCCTCAGC 
CGCCAACTTG TGGTCTCCCC ACTGGTGCCG TTCGCAGGGG ACGGGCCCAT CTCGTCAGTG 
CGCCTTCGCT ACCGGCCTCA GGACAGCTCC GCGGCCTGGT CCACCATTGT GGTGGACCCC 
AGCGAAAATG TGACCCTCAT GAACCTCCGG CCTCAGACGG GGTACAGCGT CCTGGTGCAG 
TTGAGTCGCC CAGGAGAGGG GGGCGAGGGA GCCTGGGGGT CACCCTCCAT CATGGCCACT 
GACTGCCTTG AGCCCAAGTT GCGACCCCAG CTGGAGGACT GGCATGTGGA GACCCCGGAC 
CGGATCCGAG TGACCTGGTC CTTGCCTGCC GTGCCCGGTC CCCTGGTGGG AGACGGTTTC 
CTCCTACTCC TGCGGGACCC GGAACGGGGC CACGAGCTGC GTAAGAACGT CACCTCTGCC 
CAGGCACGCT CCACTACTCT GAGCGGCCTT GTGCCCGGCA CCTTCTACCT CCTGGAAGTG 
CGGCTCTACC ATTGCACGCG CCTGGGCCCC GCTTCCCCGC CAACGCGCGT GCTCCTGCCC 
CACTCTGGCC CCCCAGCTCC ACGGCTGCTC CAGGCTGAGG CCCTGTCAGA CTCGGCCATC 
CGGCTGACCT GGCAACGTCC AGAGGACGTC GGAGCCATTG CCAAGTACAT CGTGGAGCTG 
CAGGTGGTCG GAGGGTCTGG GGAGCCCCTG TGGGTGGACA CAGAAGGAGC CGAGGAGAGC 
ACCGTCATCC ACAGCCTCAA TGCCAGCACC TGCTACCACT TCCGGGTTCG AGCCAGCGCT 
CAGGGCCCGG GAGCCTGGAG CGAAGTAGTA GAGGAAACGA CGCTGGGTAA TGGCCTGGAG 
AGCGAGGGCC CAGCCCAGCA GAGCCGGACA GCAAGCCCCA GCCTGGACCA GCAGCTGGTG 
CTGGCCATCG TGGGCTCCGT GTCGGCCACG TGCCTCACCA TCCTGGCCGC GCTGCTGGCC 
CTGGTCTGCA TCCGCAAAAG CTGCCTCCAT CGAAGACGGA CCTTCACCTA CCAGTCGGGC 
TCGGGAGAAG AGACGGTGCT TCAGTTCAGC TCCGGGACGC TGACCCTGAC CCGCCGTCCG 
AAGCCGCAGC TGGAGCCTTT GAGTTACCCC GTGCTGGAGT GGGAAGACAT CACCTTTGAG 
GACCTCATTG GGGAGGGCAA CTTCGGCCAG GTCCTTAGAG CCATGATCAA GAAGGATGGG 
CTCCAGATGA ATGCGGCCGT CAAGATGCTC AAAGAATTCG CCTCCGAGAA CGACCACCGT 
GACTTTGCCG GAGAACTGGA GGTTCTGTGC AAGCTGGGAC ACCACCCGAA CATCATCAAC 
CTGCTGGGAG CCTGCGAGAA CCGAGGATAC CTGTACATCG CCATAGAGTA CGCCCCCTAC 
GGGAACCTGC TGGACTTCCT TCGGAAGAGC CGCGTGCTGG AGACGGACCC GGCCTTCGCC 
CACGAGCACG GCACGGCCTC CACGCTCACG TCCCAGCAGC TCCTGCGCTT CGCCAGCGAC 
GTGTCCAAGG GCATGCAGTA CCTGAGCGAG AAGCAGTTCA TCCACAGGGA TCTGGCCGCC 
CGGAACGTGC TCGTGGGGGA GAATCTGGCC TCCAAGATTG CAGACTTCGG CCTGTCCCGG 
GGGGAGGAGG TCTACGTGAA GAAGACCATG GGCCGGCTCC CTGTGCGCTG GATGGCCATC 
GAGTCTCTGA ACTACAGTGT CTACACCACC AAGAGTGACG TTTGGTCCTT CGGGGTTTTG 
CTCTGGGAGA TCGTGAGCCT CGGGGGCACG CCGTACTGTG GGATGACGTG CGCTGAGCTC 
TACGAGAAGC TGCCCCAGGG TTACCGCATG GAGCAACCCC GAAATTGTGA TGACGAAGTG 
TATGAGCTGA TGCGCCAGTG CTGGAGGGAC CGTCCCTACG AAAGGCCGCC CTTTGCGCAG 
ATCTCCGTGC AGCTGGGCCG GATGTTAGAG GCCAGGAAGG CCTATGTGAA CATGGCGCTG 
TTTGAAAACT TCACCTACGC AGGTATTGAT GCGACTGCTG AGGAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031825142.1
CDS	542..3952
Translation	MGQGGPLLLLLFLLSYQVGAAMDLTLLADLRLTDPQRFFLTCVSGESGAGRGPDVWDPPLLLEKDDRIVRAFPPRQPLHSTRNGSRHITLRGFSQPSDLVGVFSCIGGTGSRRTRVVYVHNSPGAHLLPDKVTHTVNVGDTAVLSAQIRTEKQVDVIWKSNGSYFHTLDWREAQDGRFRIALPNLKASDGGIYSATYLQGNPLASAFFRVIVRGCGAGRWGPGCAKDCPSCLHGGICHDQNGECVCPPGFTGTRCEQACREGRFGQGCQQQCPGTSGCRGLTFCLPDPYGCSCGAGWGGDQCQEACSPGWFGASCYLQCRCQNGGTCDRFSGCVCPSGWHGEHCEKSDRNPEIVNMASELDFNVGAMPVVSCAATGNPPPVRGSMELRRPDGTIFMPSKAIMEVDKTTSEFQVPPLSPTDSGLWECRVSTSGGQDSRHFKVNIKVPPVPLAPPKLLARLSRQLVVSPLVPFAGDGPISSVRLRYRPQDSSAAWSTIVVDPSENVTLMNLRPQTGYSVLVQLSRPGEGGEGAWGSPSIMATDCLEPKLRPQLEDWHVETPDRIRVTWSLPAVPGPLVGDGFLLLLRDPERGHELRKNVTSAQARSTTLSGLVPGTFYLLEVRLYHCTRLGPASPPTRVLLPHSGPPAPRLLQAEALSDSAIRLTWQRPEDVGAIAKYIVELQVVGGSGEPLWVDTEGAEESTVIHSLNASTCYHFRVRASAQGPGAWSEVVEETTLGNGLESEGPAQQSRTASPSLDQQLVLAIVGSVSATCLTILAALLALVCIRKSCLHRRRTFTYQSGSGEETVLQFSSGTLTLTRRPKPQLEPLSYPVLEWEDITFEDLIGEGNFGQVLRAMIKKDGLQMNAAVKMLKEFASENDHRDFAGELEVLCKLGHHPNIINLLGACENRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAHEHGTASTLTSQQLLRFASDVSKGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQISVQLGRMLEARKAYVNMALFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_031969282.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031969282.1

ORF Insert Sequence:

ATGGGCCAAG GCGGCCCCCT TCTCCTCCTC CTCTTCCTTC TCAGCTACCA AGTGGGGGCA 
GCCATGGACC TCACGCTGCT GGCAGACCTT CGCCTCACAG ACCCCCAACG CTTTTTTCTG 
ACCTGTGTAT CTGGAGAATC TGGGGCAGGC AGGGGCCCAG ATGTCTGGGA CCCTCCCCTG 
CTCCTGGAAA AGGATGACCG AATCGTTCGG GCCTTCCCAC CTCGCCAGCC CCTCCATTCT 
ACAAGAAATG GATCTCGCCA CATCACGCTT CGGGGCTTCT CCCAGCCCTC TGACCTGGTC 
GGCGTATTTT CCTGCATTGG TGGAACCGGG TCCCGGCGCA CACGTGTGGT CTATGTACAC 
AATAGTCCTG GGGCCCACCT GCTCCCAGAC AAGGTGACTC ACACCGTGAA TGTGGGTGAC 
ACAGCTGTGC TGTCCGCTCA GATCCGCACG GAAAAACAAG TAGATGTGAT CTGGAAGAGT 
AACGGCTCCT ACTTCCACAC CCTGGACTGG CGTGAAGCCC AGGATGGGCG ATTCAGGATA 
GCGCTCCCCA ACCTGAAGGC CTCGGATGGC GGCATCTACA GCGCCACCTA CCTGCAGGGC 
AACCCCCTGG CCAGCGCCTT CTTCAGGGTC ATTGTGCGAG GCTGCGGTGC GGGGCGATGG 
GGGCCGGGCT GCGCCAAGGA CTGCCCCAGC TGCCTGCATG GAGGCATTTG CCATGACCAA 
AATGGCGAGT GTGTGTGCCC CCCTGGCTTC ACGGGCACCC GCTGCGAGCA GGCCTGCAGG 
GAGGGCCGGT TCGGGCAAGG CTGCCAGCAG CAGTGTCCGG GCACGTCCGG CTGCCGCGGA 
CTCACCTTCT GCCTCCCGGA CCCCTATGGC TGCTCCTGTG GGGCTGGCTG GGGGGGCGAC 
CAGTGCCAAG AAGCCTGTTC CCCTGGCTGG TTTGGGGCCT CCTGTTACCT GCAGTGTCGA 
TGCCAGAACG GAGGCACCTG TGACCGCTTT AGTGGCTGTG TCTGTCCCTC AGGATGGCAC 
GGGGAGCACT GCGAAAAATC AGACCGAAAC CCAGAAATCG TGAACATGGC CTCAGAGTTG 
GACTTCAACG TGGGAGCCAT GCCCGTAGTC AGCTGTGCAG CCACAGGGAA TCCGCCCCCG 
GTGCGGGGCA GCATGGAGCT ACGAAGGCCC GATGGCACCA TCTTCATGCC GTCCAAGGCC 
ATCATGGAGG TGGACAAGAC TACGTCCGAG TTCCAGGTGC CGCCCTTGAG CCCCACAGAC 
AGTGGCCTGT GGGAGTGTCG AGTGTCTACG TCCGGGGGTC AGGACAGCAG GCACTTCAAG 
GTCAACATCA AAGTTCCCCC CGTGCCCCTG GCCCCTCCGA AGCTCCTGGC CCGCCTCAGC 
CGCCAACTTG TGGTCTCCCC ACTGGTGCCG TTCGCAGGGG ACGGGCCCAT CTCGTCAGTG 
CGCCTTCGCT ACCGGCCTCA GGACAGCTCC GCGGCCTGGT CCACCATTGT GGTGGACCCC 
AGCGAAAATG TGACCCTCAT GAACCTCCGG CCTCAGACGG GGTACAGCGT CCTGGTGCAG 
TTGAGTCGCC CAGGAGAGGG GGGCGAGGGA GCCTGGGGGT CACCCTCCAT CATGGCCACT 
GACTGCCTTG AGCCCAAGTT GCGACCCCAG CTGGAGGACT GGCATGTGGA GACCCCGGAC 
CGGATCCGAG TGACCTGGTC CTTGCCTGCC GTGCCCGGTC CCCTGGTGGG AGACGGTTTC 
CTCCTACTCC TGCGGGACCC GGAACGGGGC CACGAGCTGC GTAAGAACGT CACCTCTGCC 
CAGGCACGCT CCACTACTCT GAGCGGCCTT GTGCCCGGCA CCTTCTACCT CCTGGAAGTG 
CGGCTCTACC ATTGCACGCG CCTGGGCCCC GCTTCCCCGC CAACGCGCGT GCTCCTGCCC 
CACTCTGGCC CCCCAGCTCC ACGGCTGCTC CAGGCTGAGG CCCTGTCAGA CTCGGCCATC 
CGGCTGACCT GGCAACGTCC AGAGGACGTC GGAGCCATTG CCAAGTACAT CGTGGAGCTG 
CAGGTGGTCG GAGGGTCTGG GGAGCCCCTG TGGGTGGACA CAGAAGGAGC CGAGGAGAGC 
ACCGTCATCC ACAGCCTCAA TGCCAGCACC TGCTACCACT TCCGGGTTCG AGCCAGCGCT 
CAGGGCCCGG GAGCCTGGAG CGAAGTAGTA GAGGAAACGA CGCTGGGTAA TGGCCTGGAG 
AGCGAGGGCC CAGCCCAGCA GAGCCGGACA GCAAGCCCCA GCCTGGACCA GCAGCTGGTG 
CTGGCCATCG TGGGCTCCGT GTCGGCCACG TGCCTCACCA TCCTGGCCGC GCTGCTGGCC 
CTGGTCTGCA TCCGCAAAAG CTGCCTCCAT CGAAGACGGA CCTTCACCTA CCAGTCGGGC 
TCGGGAGAAG AGACGGTGCT TCAGTTCAGC TCCGGGACGC TGACCCTGAC CCGCCGTCCG 
AAGCCGCAGC TGGAGCCTTT GAGTTACCCC GTGCTGGAGT GGGAAGACAT CACCTTTGAG 
GACCTCATTG GGGAGGGCAA CTTCGGCCAG GTCCTTAGAG CCATGATCAA GAAGGATGGG 
CTCCAGATGA ATGCGGCCGT CAAGATGCTC AAAGAATTCG CCTCCGAGAA CGACCACCGT 
GACTTTGCCG GAGAACTGGA GGTTCTGTGC AAGCTGGGAC ACCACCCGAA CATCATCAAC 
CTGCTGGGAG CCTGCGAGAA CCGAGGATAC CTGTACATCG CCATAGAGTA CGCCCCCTAC 
GGGAACCTGC TGGACTTCCT TCGGAAGAGC CGCGTGCTGG AGACGGACCC GGCCTTCGCC 
CACGAGCACG GCACGGCCTC CACGCTCACG TCCCAGCAGC TCCTGCGCTT CGCCAGCGAC 
GTGTCCAAGG GCATGCAGTA CCTGAGCGAG AAGCAGTTCA TCCACAGGGA TCTGGCCGCC 
CGGAACGTGC TCGTGGGGGA GAATCTGGCC TCCAAGATTG CAGACTTCGG CCTGTCCCGG 
GGGGAGGAGG TCTACGTGAA GAAGACCATG GGCCGGCTCC CTGTGCGCTG GATGGCCATC 
GAGTCTCTGA ACTACAGTGT CTACACCACC AAGAGTGACG TTTGGTCCTT CGGGGTTTTG 
CTCTGGGAGA TCGTGAGCCT CGGGGGCACG CCGTACTGTG GGATGACGTG CGCTGAGCTC 
TACGAGAAGC TGCCCCAGGG TTACCGCATG GAGCAACCCC GAAATTGTGA TGACGAAGTG 
TATGAGCTGA TGCGCCAGTG CTGGAGGGAC CGTCCCTACG AAAGGCCGCC CTTTGCGCAG 
ATCTCCGTGC AGCTGGGCCG GATGTTAGAG GCCAGGAAGG CCTATGTGAA CATGGCGCTG 
TTTGAAAACT TCACCTACGC AGGTATTGAT GCGACTGCTG AGGAGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_031969283.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031825143.1 Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa235828
Clone ID Related Accession (Same CDS sequence)	XM_031969283.1
Accession Version	XM_031969283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	tyrosine-protein kinase receptor Tie-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAAG GCGGCCCCCT TCTCCTCCTC CTCTTCCTTC TCAGCTACCA AGTGGGGGCA 
GCCATGGACC TCACGCTGCT GGCAGACCTT CGCCTCACAG ACCCCCAACG CTTTTTTCTG 
ACCTGTGTAT CTGGAGAATC TGGGGCAGGC AGGGGCCCAG ATGTCTGGGA CCCTCCCCTG 
CTCCTGGAAA AGGATGACCG AATCGTTCGG GCCTTCCCAC CTCGCCAGCC CCTCCATTCT 
ACAAGAAATG GATCTCGCCA CATCACGCTT CGGGGCTTCT CCCAGCCCTC TGACCTGGTC 
GGCGTATTTT CCTGCATTGG TGGAACCGGG TCCCGGCGCA CACGTGTGGT CTATGTACAC 
AATAGTCCTG GGGCCCACCT GCTCCCAGAC AAGGTGACTC ACACCGTGAA TGTGGGTGAC 
ACAGCTGTGC TGTCCGCTCA GATCCGCACG GAAAAACAAG TAGATGTGAT CTGGAAGAGT 
AACGGCTCCT ACTTCCACAC CCTGGACTGG CGTGAAGCCC AGGATGGGCG ATTCAGGATA 
GCGCTCCCCA ACCTGAAGGC CTCGGATGGC GGCATCTACA GCGCCACCTA CCTGCAGGGC 
AACCCCCTGG CCAGCGCCTT CTTCAGGGTC ATTGTGCGAG GCTGCGGTGC GGGGCGATGG 
GGGCCGGGCT GCGCCAAGGA CTGCCCCAGC TGCCTGCATG GAGGCATTTG CCATGACCAA 
AATGGCGAGT GTGTGTGCCC CCCTGGCTTC ACGGGCACCC GCTGCGAGCA GGCCTGCAGG 
GAGGGCCGGT TCGGGCAAGG CTGCCAGCAG CAGTGTCCGG GCACGTCCGG CTGCCGCGGA 
CTCACCTTCT GCCTCCCGGA CCCCTATGGC TGCTCCTGTG GGGCTGGCTG GGGGGGCGAC 
CAGTGCCAAG AAGCCTGTTC CCCTGGCTGG TTTGGGGCCT CCTGTTACCT GCAGTGTCGA 
TGCCAGAACG GAGGCACCTG TGACCGCTTT AGTGGCTGTG TCTGTCCCTC AGGATGGCAC 
GGGGAGCACT GCGAAAAATC AGACCGAAAC CCAGAAATCG TGAACATGGC CTCAGAGTTG 
GACTTCAACG TGGGAGCCAT GCCCGTAGTC AGCTGTGCAG CCACAGGGAA TCCGCCCCCG 
GTGCGGGGCA GCATGGAGCT ACGAAGGCCC GATGGCACCA TCTTCATGCC GTCCAAGGCC 
ATCATGGAGG TGGACAAGAC TACGTCCGAG TTCCAGGTGC CGCCCTTGAG CCCCACAGAC 
AGTGGCCTGT GGGAGTGTCG AGTGTCTACG TCCGGGGGTC AGGACAGCAG GCACTTCAAG 
GTCAACATCA AAGTTCCCCC CGTGCCCCTG GCCCCTCCGA AGCTCCTGGC CCGCCTCAGC 
CGCCAACTTG TGGTCTCCCC ACTGGTGCCG TTCGCAGGGG ACGGGCCCAT CTCGTCAGTG 
CGCCTTCGCT ACCGGCCTCA GGACAGCTCC GCGGCCTGGT CCACCATTGT GGTGGACCCC 
AGCGAAAATG TGACCCTCAT GAACCTCCGG CCTCAGACGG GGTACAGCGT CCTGGTGCAG 
TTGAGTCGCC CAGGAGAGGG GGGCGAGGGA GCCTGGGGGT CACCCTCCAT CATGGCCACT 
GACTGCCTTG AGCCCAAGTT GCGACCCCAG CTGGAGGACT GGCATGTGGA GACCCCGGAC 
CGGATCCGAG TGACCTGGTC CTTGCCTGCC GTGCCCGGTC CCCTGGTGGG AGACGGTTTC 
CTCCTACTCC TGCGGGACCC GGAACGGGGC CACGAGCTGC GTAAGAACGT CACCTCTGCC 
CAGGCACGCT CCACTACTCT GAGCGGCCTT GTGCCCGGCA CCTTCTACCT CCTGGAAGTG 
CGGCTCTACC ATTGCACGCG CCTGGGCCCC GCTTCCCCGC CAACGCGCGT GCTCCTGCCC 
CACTCTGGCC CCCCAGCTCC ACGGCTGCTC CAGGCTGAGG CCCTGTCAGA CTCGGCCATC 
CGGCTGACCT GGCAACGTCC AGAGGACGTC GGAGCCATTG CCAAGTACAT CGTGGAGCTG 
CAGGTGGTCG GAGGGTCTGG GGAGCCCCTG TGGGTGGACA CAGAAGGAGC CGAGGAGAGC 
ACCGTCATCC ACAGCCTCAA TGCCAGCACC TGCTACCACT TCCGGGTTCG AGCCAGCGCT 
CAGGGCCCGG GAGCCTGGAG CGAAGTAGTA GAGGAAACGA CGCTGGGCCT GGAGAGCGAG 
GGCCCAGCCC AGCAGAGCCG GACAGCAAGC CCCAGCCTGG ACCAGCAGCT GGTGCTGGCC 
ATCGTGGGCT CCGTGTCGGC CACGTGCCTC ACCATCCTGG CCGCGCTGCT GGCCCTGGTC 
TGCATCCGCA AAAGCTGCCT CCATCGAAGA CGGACCTTCA CCTACCAGTC GGGCTCGGGA 
GAAGAGACGG TGCTTCAGTT CAGCTCCGGG ACGCTGACCC TGACCCGCCG TCCGAAGCCG 
CAGCTGGAGC CTTTGAGTTA CCCCGTGCTG GAGTGGGAAG ACATCACCTT TGAGGACCTC 
ATTGGGGAGG GCAACTTCGG CCAGGTCCTT AGAGCCATGA TCAAGAAGGA TGGGCTCCAG 
ATGAATGCGG CCGTCAAGAT GCTCAAAGAA TTCGCCTCCG AGAACGACCA CCGTGACTTT 
GCCGGAGAAC TGGAGGTTCT GTGCAAGCTG GGACACCACC CGAACATCAT CAACCTGCTG 
GGAGCCTGCG AGAACCGAGG ATACCTGTAC ATCGCCATAG AGTACGCCCC CTACGGGAAC 
CTGCTGGACT TCCTTCGGAA GAGCCGCGTG CTGGAGACGG ACCCGGCCTT CGCCCACGAG 
CACGGCACGG CCTCCACGCT CACGTCCCAG CAGCTCCTGC GCTTCGCCAG CGACGTGTCC 
AAGGGCATGC AGTACCTGAG CGAGAAGCAG TTCATCCACA GGGATCTGGC CGCCCGGAAC 
GTGCTCGTGG GGGAGAATCT GGCCTCCAAG ATTGCAGACT TCGGCCTGTC CCGGGGGGAG 
GAGGTCTACG TGAAGAAGAC CATGGGCCGG CTCCCTGTGC GCTGGATGGC CATCGAGTCT 
CTGAACTACA GTGTCTACAC CACCAAGAGT GACGTTTGGT CCTTCGGGGT TTTGCTCTGG 
GAGATCGTGA GCCTCGGGGG CACGCCGTAC TGTGGGATGA CGTGCGCTGA GCTCTACGAG 
AAGCTGCCCC AGGGTTACCG CATGGAGCAA CCCCGAAATT GTGATGACGA AGTGTATGAG 
CTGATGCGCC AGTGCTGGAG GGACCGTCCC TACGAAAGGC CGCCCTTTGC GCAGATCTCC 
GTGCAGCTGG GCCGGATGTT AGAGGCCAGG AAGGCCTATG TGAACATGGC GCTGTTTGAA 
AACTTCACCT ACGCAGGTAT TGATGCGACT GCTGAGGAGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031825143.1
CDS	542..3946
Translation	MGQGGPLLLLLFLLSYQVGAAMDLTLLADLRLTDPQRFFLTCVSGESGAGRGPDVWDPPLLLEKDDRIVRAFPPRQPLHSTRNGSRHITLRGFSQPSDLVGVFSCIGGTGSRRTRVVYVHNSPGAHLLPDKVTHTVNVGDTAVLSAQIRTEKQVDVIWKSNGSYFHTLDWREAQDGRFRIALPNLKASDGGIYSATYLQGNPLASAFFRVIVRGCGAGRWGPGCAKDCPSCLHGGICHDQNGECVCPPGFTGTRCEQACREGRFGQGCQQQCPGTSGCRGLTFCLPDPYGCSCGAGWGGDQCQEACSPGWFGASCYLQCRCQNGGTCDRFSGCVCPSGWHGEHCEKSDRNPEIVNMASELDFNVGAMPVVSCAATGNPPPVRGSMELRRPDGTIFMPSKAIMEVDKTTSEFQVPPLSPTDSGLWECRVSTSGGQDSRHFKVNIKVPPVPLAPPKLLARLSRQLVVSPLVPFAGDGPISSVRLRYRPQDSSAAWSTIVVDPSENVTLMNLRPQTGYSVLVQLSRPGEGGEGAWGSPSIMATDCLEPKLRPQLEDWHVETPDRIRVTWSLPAVPGPLVGDGFLLLLRDPERGHELRKNVTSAQARSTTLSGLVPGTFYLLEVRLYHCTRLGPASPPTRVLLPHSGPPAPRLLQAEALSDSAIRLTWQRPEDVGAIAKYIVELQVVGGSGEPLWVDTEGAEESTVIHSLNASTCYHFRVRASAQGPGAWSEVVEETTLGLESEGPAQQSRTASPSLDQQLVLAIVGSVSATCLTILAALLALVCIRKSCLHRRRTFTYQSGSGEETVLQFSSGTLTLTRRPKPQLEPLSYPVLEWEDITFEDLIGEGNFGQVLRAMIKKDGLQMNAAVKMLKEFASENDHRDFAGELEVLCKLGHHPNIINLLGACENRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAHEHGTASTLTSQQLLRFASDVSKGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQISVQLGRMLEARKAYVNMALFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_031969283.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031969283.1

ORF Insert Sequence:

ATGGGCCAAG GCGGCCCCCT TCTCCTCCTC CTCTTCCTTC TCAGCTACCA AGTGGGGGCA 
GCCATGGACC TCACGCTGCT GGCAGACCTT CGCCTCACAG ACCCCCAACG CTTTTTTCTG 
ACCTGTGTAT CTGGAGAATC TGGGGCAGGC AGGGGCCCAG ATGTCTGGGA CCCTCCCCTG 
CTCCTGGAAA AGGATGACCG AATCGTTCGG GCCTTCCCAC CTCGCCAGCC CCTCCATTCT 
ACAAGAAATG GATCTCGCCA CATCACGCTT CGGGGCTTCT CCCAGCCCTC TGACCTGGTC 
GGCGTATTTT CCTGCATTGG TGGAACCGGG TCCCGGCGCA CACGTGTGGT CTATGTACAC 
AATAGTCCTG GGGCCCACCT GCTCCCAGAC AAGGTGACTC ACACCGTGAA TGTGGGTGAC 
ACAGCTGTGC TGTCCGCTCA GATCCGCACG GAAAAACAAG TAGATGTGAT CTGGAAGAGT 
AACGGCTCCT ACTTCCACAC CCTGGACTGG CGTGAAGCCC AGGATGGGCG ATTCAGGATA 
GCGCTCCCCA ACCTGAAGGC CTCGGATGGC GGCATCTACA GCGCCACCTA CCTGCAGGGC 
AACCCCCTGG CCAGCGCCTT CTTCAGGGTC ATTGTGCGAG GCTGCGGTGC GGGGCGATGG 
GGGCCGGGCT GCGCCAAGGA CTGCCCCAGC TGCCTGCATG GAGGCATTTG CCATGACCAA 
AATGGCGAGT GTGTGTGCCC CCCTGGCTTC ACGGGCACCC GCTGCGAGCA GGCCTGCAGG 
GAGGGCCGGT TCGGGCAAGG CTGCCAGCAG CAGTGTCCGG GCACGTCCGG CTGCCGCGGA 
CTCACCTTCT GCCTCCCGGA CCCCTATGGC TGCTCCTGTG GGGCTGGCTG GGGGGGCGAC 
CAGTGCCAAG AAGCCTGTTC CCCTGGCTGG TTTGGGGCCT CCTGTTACCT GCAGTGTCGA 
TGCCAGAACG GAGGCACCTG TGACCGCTTT AGTGGCTGTG TCTGTCCCTC AGGATGGCAC 
GGGGAGCACT GCGAAAAATC AGACCGAAAC CCAGAAATCG TGAACATGGC CTCAGAGTTG 
GACTTCAACG TGGGAGCCAT GCCCGTAGTC AGCTGTGCAG CCACAGGGAA TCCGCCCCCG 
GTGCGGGGCA GCATGGAGCT ACGAAGGCCC GATGGCACCA TCTTCATGCC GTCCAAGGCC 
ATCATGGAGG TGGACAAGAC TACGTCCGAG TTCCAGGTGC CGCCCTTGAG CCCCACAGAC 
AGTGGCCTGT GGGAGTGTCG AGTGTCTACG TCCGGGGGTC AGGACAGCAG GCACTTCAAG 
GTCAACATCA AAGTTCCCCC CGTGCCCCTG GCCCCTCCGA AGCTCCTGGC CCGCCTCAGC 
CGCCAACTTG TGGTCTCCCC ACTGGTGCCG TTCGCAGGGG ACGGGCCCAT CTCGTCAGTG 
CGCCTTCGCT ACCGGCCTCA GGACAGCTCC GCGGCCTGGT CCACCATTGT GGTGGACCCC 
AGCGAAAATG TGACCCTCAT GAACCTCCGG CCTCAGACGG GGTACAGCGT CCTGGTGCAG 
TTGAGTCGCC CAGGAGAGGG GGGCGAGGGA GCCTGGGGGT CACCCTCCAT CATGGCCACT 
GACTGCCTTG AGCCCAAGTT GCGACCCCAG CTGGAGGACT GGCATGTGGA GACCCCGGAC 
CGGATCCGAG TGACCTGGTC CTTGCCTGCC GTGCCCGGTC CCCTGGTGGG AGACGGTTTC 
CTCCTACTCC TGCGGGACCC GGAACGGGGC CACGAGCTGC GTAAGAACGT CACCTCTGCC 
CAGGCACGCT CCACTACTCT GAGCGGCCTT GTGCCCGGCA CCTTCTACCT CCTGGAAGTG 
CGGCTCTACC ATTGCACGCG CCTGGGCCCC GCTTCCCCGC CAACGCGCGT GCTCCTGCCC 
CACTCTGGCC CCCCAGCTCC ACGGCTGCTC CAGGCTGAGG CCCTGTCAGA CTCGGCCATC 
CGGCTGACCT GGCAACGTCC AGAGGACGTC GGAGCCATTG CCAAGTACAT CGTGGAGCTG 
CAGGTGGTCG GAGGGTCTGG GGAGCCCCTG TGGGTGGACA CAGAAGGAGC CGAGGAGAGC 
ACCGTCATCC ACAGCCTCAA TGCCAGCACC TGCTACCACT TCCGGGTTCG AGCCAGCGCT 
CAGGGCCCGG GAGCCTGGAG CGAAGTAGTA GAGGAAACGA CGCTGGGCCT GGAGAGCGAG 
GGCCCAGCCC AGCAGAGCCG GACAGCAAGC CCCAGCCTGG ACCAGCAGCT GGTGCTGGCC 
ATCGTGGGCT CCGTGTCGGC CACGTGCCTC ACCATCCTGG CCGCGCTGCT GGCCCTGGTC 
TGCATCCGCA AAAGCTGCCT CCATCGAAGA CGGACCTTCA CCTACCAGTC GGGCTCGGGA 
GAAGAGACGG TGCTTCAGTT CAGCTCCGGG ACGCTGACCC TGACCCGCCG TCCGAAGCCG 
CAGCTGGAGC CTTTGAGTTA CCCCGTGCTG GAGTGGGAAG ACATCACCTT TGAGGACCTC 
ATTGGGGAGG GCAACTTCGG CCAGGTCCTT AGAGCCATGA TCAAGAAGGA TGGGCTCCAG 
ATGAATGCGG CCGTCAAGAT GCTCAAAGAA TTCGCCTCCG AGAACGACCA CCGTGACTTT 
GCCGGAGAAC TGGAGGTTCT GTGCAAGCTG GGACACCACC CGAACATCAT CAACCTGCTG 
GGAGCCTGCG AGAACCGAGG ATACCTGTAC ATCGCCATAG AGTACGCCCC CTACGGGAAC 
CTGCTGGACT TCCTTCGGAA GAGCCGCGTG CTGGAGACGG ACCCGGCCTT CGCCCACGAG 
CACGGCACGG CCTCCACGCT CACGTCCCAG CAGCTCCTGC GCTTCGCCAG CGACGTGTCC 
AAGGGCATGC AGTACCTGAG CGAGAAGCAG TTCATCCACA GGGATCTGGC CGCCCGGAAC 
GTGCTCGTGG GGGAGAATCT GGCCTCCAAG ATTGCAGACT TCGGCCTGTC CCGGGGGGAG 
GAGGTCTACG TGAAGAAGAC CATGGGCCGG CTCCCTGTGC GCTGGATGGC CATCGAGTCT 
CTGAACTACA GTGTCTACAC CACCAAGAGT GACGTTTGGT CCTTCGGGGT TTTGCTCTGG 
GAGATCGTGA GCCTCGGGGG CACGCCGTAC TGTGGGATGA CGTGCGCTGA GCTCTACGAG 
AAGCTGCCCC AGGGTTACCG CATGGAGCAA CCCCGAAATT GTGATGACGA AGTGTATGAG 
CTGATGCGCC AGTGCTGGAG GGACCGTCCC TACGAAAGGC CGCCCTTTGC GCAGATCTCC 
GTGCAGCTGG GCCGGATGTT AGAGGCCAGG AAGGCCTATG TGAACATGGC GCTGTTTGAA 
AACTTCACCT ACGCAGGTAT TGATGCGACT GCTGAGGAGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIE1 ( XM_031969284.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031825144.1 Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X3, mRNA.

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CloneID	OSa235829
Clone ID Related Accession (Same CDS sequence)	XM_031969284.1
Accession Version	XM_031969284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	tyrosine-protein kinase receptor Tie-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCAAG GCGGCCCCCT TCTCCTCCTC CTCTTCCTTC TCAGCTACCA AGTGGGGGCA 
GCCATGGACC TCACGCTGCT GGCAGACCTT CGCCTCACAG ACCCCCAACG CTTTTTTCTG 
ACCTGTGTAT CTGGAGAATC TGGGGCAGGC AGGGGCCCAG ATGTCTGGGA CCCTCCCCTG 
CTCCTGGAAA AGGATGACCG AATCGTTCGG GCCTTCCCAC CTCGCCAGCC CCTCCATTCT 
ACAAGAAATG GATCTCGCCA CATCACGCTT CGGGGCTTCT CCCAGCCCTC TGACCTGGTC 
GGCGTATTTT CCTGCATTGG TGGAACCGGG TCCCGGCGCA CACGTGTGGT CTATGTACAC 
AATAGTCCTG GGGCCCACCT GCTCCCAGAC AAGGTGACTC ACACCGTGAA TGTGGGTGAC 
ACAGCTGTGC TGTCCGCTCA GATCCGCACG GAAAAACAAG TAGATGTGAT CTGGAAGAGT 
AACGGCTCCT ACTTCCACAC CCTGGACTGG CGTGAAGCCC AGGATGGGCG ATTCAGGATA 
GCGCTCCCCA ACCTGAAGGC CTCGGATGGC GGCATCTACA GCGCCACCTA CCTGCAGGGC 
AACCCCCTGG CCAGCGCCTT CTTCAGGGTC ATTGTGCGAG GCTGCGGTGC GGGGCGATGG 
GGGCCGGGCT GCGCCAAGGA CTGCCCCAGC TGCCTGCATG GAGGCATTTG CCATGACCAA 
AATGGCGAGT GTGTGTGCCC CCCTGGCTTC ACGGGCACCC GCTGCGAGCA GGCCTGCAGG 
GAGGGCCGGT TCGGGCAAGG CTGCCAGCAG CAGTGTCCGG GCACGTCCGG CTGCCGCGGA 
CTCACCTTCT GCCTCCCGGA CCCCTATGGC TGCTCCTGTG GGGCTGGCTG GGGGGGCGAC 
CAGTGCCAAG AAGCCTGTTC CCCTGGCTGG TTTGGGGCCT CCTGTTACCT GCAGTGTCGA 
TGCCAGAACG GAGGCACCTG TGACCGCTTT AGTGGCTGTG TCTGTCCCTC AGGATGGCAC 
GGGGAGCACT GCGAAAAATC AGACCGAAAC CCAGAAATCG TGAACATGGC CTCAGAGTTG 
GACTTCAACG TGGGAGCCAT GCCCGTAGTC AGCTGTGCAG CCACAGGGAA TCCGCCCCCG 
GTGCGGGGCA GCATGGAGCT ACGAAGGCCC GATGGCACCA TCTTCATGCC GTCCAAGGCC 
ATCATGGAGG TGGACAAGAC TACGTCCGAG TTCCAGGTGC CGCCCTTGAG CCCCACAGAC 
AGTGGCCTGT GGGAGTGTCG AGTGTCTACG TCCGGGGGTC AGGACAGCAG GCACTTCAAG 
GTCAACATCA AAGTTCCCCC CGTGCCCCTG GCCCCTCCGA AGCTCCTGGC CCGCCTCAGC 
CGCCAACTTG TGGTCTCCCC ACTGGTGCCG TTCGCAGGGG ACGGGCCCAT CTCGTCAGTG 
CGCCTTCGCT ACCGGCCTCA GGACAGCTCC GCGGCCTGGT CCACCATTGT GGTGGACCCC 
AGCGAAAATG TGACCCTCAT GAACCTCCGG CCTCAGACGG GGTACAGCGT CCTGGTGCAG 
TTGAGTCGCC CAGGAGAGGG GGGCGAGGGA GCCTGGGGGT CACCCTCCAT CATGGCCACT 
GACTGCCTTG AGCCCAAGTT GCGACCCCAG CTGGAGGACT GGCATGTGGA GACCCCGGAC 
CGGATCCGAG TGACCTGGTC CTTGCCTGCC GTGCCCGGTC CCCTGGTGGG AGACGGTTTC 
CTCCTACTCC TGCGGGACCC GGAACGGGGC CACGAGCTGC GTAAGAACGT CACCTCTGCC 
CAGGCACGCT CCACTACTCT GAGCGGCCTT GTGCCCGGCA CCTTCTACCT CCTGGAAGTG 
CGGCTCTACC ATTGCACGCG CCTGGGCCCC GCTTCCCCGC CAACGCGCGT GCTCCTGCCC 
CACTCTGGCC CCCCAGCTCC ACGGCTGCTC CAGGCTGAGG CCCTGTCAGA CTCGGCCATC 
CGGCTGACCT GGCAACGTCC AGAGGACGTC GGAGCCATTG CCAAGTACAT CGTGGAGCTG 
CAGGTGGTCG GAGGGTCTGG GGAGCCCCTG TGGGTGGACA CAGAAGGAGC CGAGGAGAGC 
ACCGTCATCC ACAGCCTCAA TGCCAGCACC TGCTACCACT TCCGGGTTCG AGCCAGCGCT 
CAGGGCCCGG GAGCCTGGAG CGAAGTAGTA GAGGAAACGA CGCTGGGTAA TGGCCTGGAG 
AGCGAGGGCC CAGCCCAGCA GAGCCGGACA GCAAGCCCCA GCCTGGACCA GCAGCTGGTG 
CTGGCCATCG TGGGCTCCGT GTCGGCCACG TGCCTCACCA TCCTGGCCGC GCTGCTGGCC 
CTGGTCTGCA TCCGCAAAAG CTGCCTCCAT CGAAGACGGA CCTTCACCTA CCAGTCGGGC 
TCGGGAGAAG AGACGGTGCT TCAGTTCAGC TCCGGGACGC TGACCCTGAC CCGCCGTCCG 
AAGCCGCAGC TGGAGCCTTT GAGTTACCCC GTGCTGGAGT GGGAAGACAT CACCTTTGAG 
GACCTCATTG GGGAGGGCAA CTTCGGCCAG GTCCTTAGAG CCATGATCAA GAAGGATGGG 
CTCCAGATGA ATGCGGCCGT CAAGATGCTC AAAGAATTCG CCTCCGAGAA CGACCACCGT 
GACTTTGCCG GAGAACTGGA GGTTCTGTGC AAGCTGGGAC ACCACCCGAA CATCATCAAC 
CTGCTGGGAG CCTGCGAGAA CCGAGGATAC CTGTACATCG CCATAGAGTA CGCCCCCTAC 
GGGAACCTGC TGGACTTCCT TCGGAAGAGC CGCGTGCTGG AGACGGACCC GGCCTTCGCC 
CACGAGCACG GCACGGCCTC CACGCTCACG TCCCAGCAGC TCCTGCGCTT CGCCAGCGAC 
GTGTCCAAGG GCATGCAGTA CCTGAGCGAG AAGCAGTTCA TCCACAGGGA TCTGGCCGCC 
CGGAACGTGC TCGTGGGGGA GAATCTGGCC TCCAAGATTG CAGACTTCGG CCTGTCCCGG 
GGGGAGGAGG TCTACGTGAA GAAGACCATG GGCCGGCTCC CTGTGCGCTG GATGGCCATC 
GAGTCTCTGA ACTACAGTGT CTACACCACC AAGAGTGACG TTTGGTCCTT CGGGGTTTTG 
CTCTGGGAGA TCGTGAGCCT CGGTATGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031825144.1
CDS	544..3696
Translation	MGQGGPLLLLLFLLSYQVGAAMDLTLLADLRLTDPQRFFLTCVSGESGAGRGPDVWDPPLLLEKDDRIVRAFPPRQPLHSTRNGSRHITLRGFSQPSDLVGVFSCIGGTGSRRTRVVYVHNSPGAHLLPDKVTHTVNVGDTAVLSAQIRTEKQVDVIWKSNGSYFHTLDWREAQDGRFRIALPNLKASDGGIYSATYLQGNPLASAFFRVIVRGCGAGRWGPGCAKDCPSCLHGGICHDQNGECVCPPGFTGTRCEQACREGRFGQGCQQQCPGTSGCRGLTFCLPDPYGCSCGAGWGGDQCQEACSPGWFGASCYLQCRCQNGGTCDRFSGCVCPSGWHGEHCEKSDRNPEIVNMASELDFNVGAMPVVSCAATGNPPPVRGSMELRRPDGTIFMPSKAIMEVDKTTSEFQVPPLSPTDSGLWECRVSTSGGQDSRHFKVNIKVPPVPLAPPKLLARLSRQLVVSPLVPFAGDGPISSVRLRYRPQDSSAAWSTIVVDPSENVTLMNLRPQTGYSVLVQLSRPGEGGEGAWGSPSIMATDCLEPKLRPQLEDWHVETPDRIRVTWSLPAVPGPLVGDGFLLLLRDPERGHELRKNVTSAQARSTTLSGLVPGTFYLLEVRLYHCTRLGPASPPTRVLLPHSGPPAPRLLQAEALSDSAIRLTWQRPEDVGAIAKYIVELQVVGGSGEPLWVDTEGAEESTVIHSLNASTCYHFRVRASAQGPGAWSEVVEETTLGNGLESEGPAQQSRTASPSLDQQLVLAIVGSVSATCLTILAALLALVCIRKSCLHRRRTFTYQSGSGEETVLQFSSGTLTLTRRPKPQLEPLSYPVLEWEDITFEDLIGEGNFGQVLRAMIKKDGLQMNAAVKMLKEFASENDHRDFAGELEVLCKLGHHPNIINLLGACENRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAHEHGTASTLTSQQLLRFASDVSKGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGMS

Target ORF information:

RefSeq Version	XM_031969284.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii tyrosine kinase with immunoglobulin like and EGF like domains 1 (TIE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031969284.1

ORF Insert Sequence:

ATGGGCCAAG GCGGCCCCCT TCTCCTCCTC CTCTTCCTTC TCAGCTACCA AGTGGGGGCA 
GCCATGGACC TCACGCTGCT GGCAGACCTT CGCCTCACAG ACCCCCAACG CTTTTTTCTG 
ACCTGTGTAT CTGGAGAATC TGGGGCAGGC AGGGGCCCAG ATGTCTGGGA CCCTCCCCTG 
CTCCTGGAAA AGGATGACCG AATCGTTCGG GCCTTCCCAC CTCGCCAGCC CCTCCATTCT 
ACAAGAAATG GATCTCGCCA CATCACGCTT CGGGGCTTCT CCCAGCCCTC TGACCTGGTC 
GGCGTATTTT CCTGCATTGG TGGAACCGGG TCCCGGCGCA CACGTGTGGT CTATGTACAC 
AATAGTCCTG GGGCCCACCT GCTCCCAGAC AAGGTGACTC ACACCGTGAA TGTGGGTGAC 
ACAGCTGTGC TGTCCGCTCA GATCCGCACG GAAAAACAAG TAGATGTGAT CTGGAAGAGT 
AACGGCTCCT ACTTCCACAC CCTGGACTGG CGTGAAGCCC AGGATGGGCG ATTCAGGATA 
GCGCTCCCCA ACCTGAAGGC CTCGGATGGC GGCATCTACA GCGCCACCTA CCTGCAGGGC 
AACCCCCTGG CCAGCGCCTT CTTCAGGGTC ATTGTGCGAG GCTGCGGTGC GGGGCGATGG 
GGGCCGGGCT GCGCCAAGGA CTGCCCCAGC TGCCTGCATG GAGGCATTTG CCATGACCAA 
AATGGCGAGT GTGTGTGCCC CCCTGGCTTC ACGGGCACCC GCTGCGAGCA GGCCTGCAGG 
GAGGGCCGGT TCGGGCAAGG CTGCCAGCAG CAGTGTCCGG GCACGTCCGG CTGCCGCGGA 
CTCACCTTCT GCCTCCCGGA CCCCTATGGC TGCTCCTGTG GGGCTGGCTG GGGGGGCGAC 
CAGTGCCAAG AAGCCTGTTC CCCTGGCTGG TTTGGGGCCT CCTGTTACCT GCAGTGTCGA 
TGCCAGAACG GAGGCACCTG TGACCGCTTT AGTGGCTGTG TCTGTCCCTC AGGATGGCAC 
GGGGAGCACT GCGAAAAATC AGACCGAAAC CCAGAAATCG TGAACATGGC CTCAGAGTTG 
GACTTCAACG TGGGAGCCAT GCCCGTAGTC AGCTGTGCAG CCACAGGGAA TCCGCCCCCG 
GTGCGGGGCA GCATGGAGCT ACGAAGGCCC GATGGCACCA TCTTCATGCC GTCCAAGGCC 
ATCATGGAGG TGGACAAGAC TACGTCCGAG TTCCAGGTGC CGCCCTTGAG CCCCACAGAC 
AGTGGCCTGT GGGAGTGTCG AGTGTCTACG TCCGGGGGTC AGGACAGCAG GCACTTCAAG 
GTCAACATCA AAGTTCCCCC CGTGCCCCTG GCCCCTCCGA AGCTCCTGGC CCGCCTCAGC 
CGCCAACTTG TGGTCTCCCC ACTGGTGCCG TTCGCAGGGG ACGGGCCCAT CTCGTCAGTG 
CGCCTTCGCT ACCGGCCTCA GGACAGCTCC GCGGCCTGGT CCACCATTGT GGTGGACCCC 
AGCGAAAATG TGACCCTCAT GAACCTCCGG CCTCAGACGG GGTACAGCGT CCTGGTGCAG 
TTGAGTCGCC CAGGAGAGGG GGGCGAGGGA GCCTGGGGGT CACCCTCCAT CATGGCCACT 
GACTGCCTTG AGCCCAAGTT GCGACCCCAG CTGGAGGACT GGCATGTGGA GACCCCGGAC 
CGGATCCGAG TGACCTGGTC CTTGCCTGCC GTGCCCGGTC CCCTGGTGGG AGACGGTTTC 
CTCCTACTCC TGCGGGACCC GGAACGGGGC CACGAGCTGC GTAAGAACGT CACCTCTGCC 
CAGGCACGCT CCACTACTCT GAGCGGCCTT GTGCCCGGCA CCTTCTACCT CCTGGAAGTG 
CGGCTCTACC ATTGCACGCG CCTGGGCCCC GCTTCCCCGC CAACGCGCGT GCTCCTGCCC 
CACTCTGGCC CCCCAGCTCC ACGGCTGCTC CAGGCTGAGG CCCTGTCAGA CTCGGCCATC 
CGGCTGACCT GGCAACGTCC AGAGGACGTC GGAGCCATTG CCAAGTACAT CGTGGAGCTG 
CAGGTGGTCG GAGGGTCTGG GGAGCCCCTG TGGGTGGACA CAGAAGGAGC CGAGGAGAGC 
ACCGTCATCC ACAGCCTCAA TGCCAGCACC TGCTACCACT TCCGGGTTCG AGCCAGCGCT 
CAGGGCCCGG GAGCCTGGAG CGAAGTAGTA GAGGAAACGA CGCTGGGTAA TGGCCTGGAG 
AGCGAGGGCC CAGCCCAGCA GAGCCGGACA GCAAGCCCCA GCCTGGACCA GCAGCTGGTG 
CTGGCCATCG TGGGCTCCGT GTCGGCCACG TGCCTCACCA TCCTGGCCGC GCTGCTGGCC 
CTGGTCTGCA TCCGCAAAAG CTGCCTCCAT CGAAGACGGA CCTTCACCTA CCAGTCGGGC 
TCGGGAGAAG AGACGGTGCT TCAGTTCAGC TCCGGGACGC TGACCCTGAC CCGCCGTCCG 
AAGCCGCAGC TGGAGCCTTT GAGTTACCCC GTGCTGGAGT GGGAAGACAT CACCTTTGAG 
GACCTCATTG GGGAGGGCAA CTTCGGCCAG GTCCTTAGAG CCATGATCAA GAAGGATGGG 
CTCCAGATGA ATGCGGCCGT CAAGATGCTC AAAGAATTCG CCTCCGAGAA CGACCACCGT 
GACTTTGCCG GAGAACTGGA GGTTCTGTGC AAGCTGGGAC ACCACCCGAA CATCATCAAC 
CTGCTGGGAG CCTGCGAGAA CCGAGGATAC CTGTACATCG CCATAGAGTA CGCCCCCTAC 
GGGAACCTGC TGGACTTCCT TCGGAAGAGC CGCGTGCTGG AGACGGACCC GGCCTTCGCC 
CACGAGCACG GCACGGCCTC CACGCTCACG TCCCAGCAGC TCCTGCGCTT CGCCAGCGAC 
GTGTCCAAGG GCATGCAGTA CCTGAGCGAG AAGCAGTTCA TCCACAGGGA TCTGGCCGCC 
CGGAACGTGC TCGTGGGGGA GAATCTGGCC TCCAAGATTG CAGACTTCGG CCTGTCCCGG 
GGGGAGGAGG TCTACGTGAA GAAGACCATG GGCCGGCTCC CTGTGCGCTG GATGGCCATC 
GAGTCTCTGA ACTACAGTGT CTACACCACC AAGAGTGACG TTTGGTCCTT CGGGGTTTTG 
CTCTGGGAGA TCGTGAGCCT CGGTATGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TIE1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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