KDM3B cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	KDM3B
Entrez Gene ID	100924702
Full Name	lysine demethylase 3B
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012550688.1	XP_012406142.1	lysine-specific demethylase 3B
XM_023504799.1	XP_023360567.1	lysine-specific demethylase 3B isoform X2
XM_031953343.1	XP_031809203.1	lysine-specific demethylase 3B isoform X2
XM_012550688.2	XP_012406142.1	lysine-specific demethylase 3B isoform X1
XM_031953342.1	XP_031809202.1	lysine-specific demethylase 3B isoform X1
XM_031953344.1	XP_031809204.1	lysine-specific demethylase 3B isoform X3

Pathways from BioSystems
Gene Ontology

The following KDM3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KDM3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa118100	XM_012550688.2 Latest version!	Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa149910	XM_023504799.1 Latest version!	Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa225682	XM_031953342.1 Latest version!	Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa225683	XM_031953343.1 Latest version!	Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa225684	XM_031953344.1 Latest version!	Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OSa118100	XM_012550688.1	Sarcophilus harrisii lysine (K)-specific demethylase 3B (KDM3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KDM3B ( XM_012550688.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012406142.1 Sarcophilus harrisii lysine (K)-specific demethylase 3B (KDM3B), mRNA.

Price & Availability↑

CloneID	OSa118100
Clone ID Related Accession (Same CDS sequence)	XM_012550688.1 , XM_012550688.2
Accession Version	XM_012550688.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	lysine-specific demethylase 3B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816604.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCTC CAGGCCAGAG ACTAAACCTG GGAACTGGGA TATTTGTGGA ATTTGATGGC 
TGCAACTGGA AGCAACACTC CTGGGTGAAA GTCCATGCTG AAGAGGTTAT TGTGCTGCTC 
CTAGAAGGCT CTTTAGTTTG GGCTCCTCGA AAGGATCCAT TCCTCCTCCA GGGGACCAGG 
GTCTCAATTG CACAGTGGCC AGCCCTGACC TTCACTCCAT TAGTGGATAA ATTGGGTTTG 
GGGTCTGTGG TTCCAGTGGA ATTTCTTCTG GATCGAGATC TCCGATTCTT GTCAGATGCT 
ACTGGATTAC GCCTATTCCA GATGGGAACA GAGAGCCAGA ACCAGATTCT TCTAGAACAT 
GCTGCATTGA GAGATACAGT TAATGCTTTG ATCAGTGACC AAAAGCTCCA GGAAATATTC 
AGTCGAGGTC CCTATAGTGT CAAAGGCCAG AGGGTCAAAG TGTACCAGCC AGAAGGTGAA 
GAAGGTTGGA TCTGTGGTGT TGTAAGCCGA CAAGACTCTC TCACTCGTCT TATGGAGGTA 
TCTTTGTCAG AGAGTGGTGA GATTAAATCA GTGGATCCCA GATTGATACA TGTGATGTTG 
ATGGACAACT CTGCTTCTCA GAATGAGGGA AGCACATTAA AAGCTGTAAA ACCCCCCAAA 
GGAAAGAAGA AAAGAGAGAG CATAGAGGGA AAAGATGGAC GAAGGAGGAA AAGTGCTTCA 
GACTCTGGGT GTGACCCTGC AACAAAGAAA TTAAAAGGAG AAAGGAGTGA AGTAGACAGT 
AATGGGAGTG ATGGAAGTGA AACAAGTCGA GGGCCCTGGA AAGGGAGTTC TAGTGGAGAG 
CCAGGGCTGG ATCAAAGGGC CAAGCAGCTG CCTCCATTTG TTCCCCAGAT CAATCGAAAC 
ATTCGATTTG CCACATACAC CAAAGAGAAT GGCAGGACTC TGGTGGTTCA GGATGAGCCT 
CTAGGTGGGG ACATGCCTGT ACCTTTCACT CCATATTCTT CAGCCACGGG GCAGTCACCT 
TTGGTTACAG AGGTGGGTGG AGCTGGAAAC AAGGAGGCTG GAAAATCACT GGATTCAGGT 
GCTCAGAGTC TGGCAGTTTC AACAGCCGTG ATCACAACCA CCAGCTCAAC ACCAACCACC 
GTGAGAATCT CTGATACAGG CCTTTCAGCA GTGACCCTGC AGGAGAAACA AAAAGTCAAC 
TGGTCTCAGA CTCAGGGAGA GAATTCCAGA AACCCTCTCT TGGCTTCTTC TGGATTTGGA 
GCATCTCTCT CAAGTTCTTC CCAATCCTTG GCTTTTGGAA GTGTTAGAGC CCAGTCCAAT 
GGGGTCTTGG CTACAGAGAG CAAGCCTTTG GGGTTTTCTT TTGCTTCCAG TTCTGCTACA 
GAAACCCAGA AAGATTCTGA TCTTTCAAAG AACTTGTTTT TTCAGTGCAT GTCACAGACT 
TTGCCCTCAA GTAACTACTT CACAGCTATT TCAGAGAGTT TATCTGATGA ATCCTCTGCT 
CGGGAGTCAT TCAAACAGAG CCTAGAGAGC CTGAGCTCTG GGCTTTGTAA AGGCAAACCT 
ACCCTGGCAG CAGATACGAA GGCTGGCAGT GCCCTGGATC GAAAGATGCC TGCAGAGTCC 
ATGCCTACCC TCACTCCAGC CTTCCCAAGG AGCCTCTTGA ATACCCGAGC CCCAGAGAAT 
CATGAAAATC TATTTTTGCA GCCCCCTAAA TTGTCCCGTG AAGAGCCATC CAACCCTTTC 
CTGGCATTTG CAGAGAAAGC TGATCTCAGT CCTTTCAGCA GTTTTACATC TCAGACAGCA 
GGTGGCTCCA CTTCTGCTAC CACTGGAGCA CTCAAAGCAG CTTCTAGCTG GCCAGAGTCT 
CACACCTCTG CTGATTCAGC ATCTTTAGCA AAGAAGAAAT CTCTCTTCAT CACAACTGAT 
TCTTCCAAGC TAGGCTCTAG TGCACCTAGC TCAGCTGTGC CTCCTGGTGT CCCAAGCCTC 
TCTGCCATGG GAAATGGCCG CTCTAATTCA CCCACCAGCA GTCTTTCACA ACCTATTGAG 
ATGCCCACCC TCTCCTCCAG CCCAACAGAG GAAAAGCCAT CAGTTGGACC TGGGCAACAG 
GACAATCCCC TTTTGAAAAC CTTTACTAAT GTCTTTGGCA GGCACCCAAC TGGCTTTCTC 
TCCTCAACAG AGTTTTTACA TGATAACAAA GCTCCTTTTG AAGCTGTAAA AAGGTTTTCA 
CTGGATGAGC GAAGTATGAC ATCTAAACAG GACTCAGATT CCAGCACCAA TAGTGACCTG 
TCAGACCTGA GTGACTCTGA GGAGCAGCTG CAAGCCAAGG CTGGGTTGAA GGGAATCCCC 
GAGCACCTAA TGGGAAAGCT GGGCCCCAAT GGAGAGCGAA ATGCTGAACT ATTACTGGGA 
AAAGGAAGAG GGAAGCAGGC TCCTAAAGGC CGACCTCGAA CAGCCCCTCT GAAAGTTGGC 
CAATCTGTGC TAAAAGACAT GAGCAAGGTA AGAAAGCTCA AACAGTCAGG AGAGCCTTTC 
CTCCAAGATG GATCCTGCAT TAATGTGGCC CCTCACCTCC ATAAGTGCAG GGAGTGCCGC 
CTGGAGCGCT ACCGAAAGTT TAAAGAGCAA GAACAAGATG ATTCCACTGT GGCCTGCCGA 
TTCTTTCACT TCCGCAGGCT TATCTTCACT CGGAAAGGCA TCCTCCGTGT AGAAGGGTTT 
TTGAGTCCGC AGCAAAGTGA TCCAGAAGCC ATGAATCTGT GGATTCCCTC TTCCTCCCTT 
GCAGAAGGAA TCGATCTGGA GACCTCAAAA TACATACTAG CCAATGTCGG GGACCAGTTT 
TGCCAGCTTG TCATGTCAGA AAAGGAGGCC ATGATGATGG TAGAGCCACA CCAAAAAGTG 
GCATGGAAAC GAGCTGTACG TGGTGTCAGG GAAATGTGTG ATGTATGTGA AACAACTCTC 
TTCAACATCC ATTGGGTTTG TCGCAAATGT GGATTTGGAG TCTGTCTGGA CTGTTACCGA 
CTTAGAAAGA GTCGTCCACG TAGTGAGACC GAAGAGATTG GTGATGAAGA GGTTTTCTCT 
TGGTTGAAAT GTGCAAAGGG GCAGTCACAT GAACCAGAAA ATCTCATGCC TACACAAATC 
ATCCCTGGCA CAGCTCTTTA CAATATTGGA GATATGGTAC ATGCAGCCCG GGGCAAGTGG 
GGAATTAAAG CCAATTGTCC TTGCATTAGT CGTCAGAATA AATCTGTATT GAGGCCTGCT 
GTCACTAATG GGATTTCACA GCTTCCCAGT ATAAATCCCA GTGCTTCCTC TTCTGGAAGT 
GAAACTCCCT TCTCCAGTGG AGGAGGGACA ACAGGGGTGA CAACACCAGA GTCTGACCTA 
TTACTCAAAC CTGACACTCT GGACAGCAGG GGTGAGGAGG CACTGAAAAC TGATGCATCA 
AGTAGCAGTA GTGAGACAAA AACCAGCAGG CCACTGTGCC CCGAAACAAC ACCACCATCC 
TCTGCTTTGC ACTGGCTGGC AGATTTAGCA ACACAGAAAG CAAAGGAAGA AACAAAAGAA 
GCTGGATCCC TGAGATCAGT ACTCAGTAAG GAGTCTCATT CCCCCTTTGG GCTGGATTCC 
TTCAACTCTA CAGCAAAGGT TTCTCCATTA ACTCCAAAGC TCTTTAATAG TCTGCTACTG 
GGTCCTGCTG CCTCAAACAA CAAAACAGAA GGCTCCAGCC TTCGAGACCT ACTACACTCT 
GGACCCGGAA AGCTTCCCCA GGCCCCCTTG GACACCAGCA TACCTTTCCC TCCAGTCTTC 
TCAGCATCTT CAACAGGCGT GAAGAATAAG GCCAGTCTTC CCAACTTCCT CGACCACATC 
ATTGCCTCAG TGGTAGAAAA TAAAAAAACT TCTGATGCTG CAAAACGGGC CAGTAACTTG 
ACAGATACCC AAAAAGAAGT GAAGGAGATG GTAATGGGGC TGAATGTGTT GGATCCACAC 
ACCTCCCACT CATGGCTCTG TGATGGGAGG CTCCTATGTC TCCATGACCC CAGCAACAAA 
AACAACTGGA AGATCTTTCG GGAGTGTTGG AAGCAAGGCC AGCCTGTCCT TGTTTCCGGG 
GTGCATAAGA AGCTCAAATC TGAACTTTGG AAGCCAGAAG CATTTAGTCA AGAATTTGGA 
GACCAGGATG TGGACTTAGT AAACTGTAGG AACTGTGCTA TCATTTCTGA TGTGAAAGTG 
CGTGACTTCT GGGATGGTTT CGAAATCATA TGCAAACGGC TAAGGTCAGA AGATGGGCAG 
CCAATGGTAC TGAAACTCAA AGACTGGCCT CCAGGGGAAG ATTTCCGAGA CATGATGCCT 
ACAAGGTTTG AGGATCTGAT GGAGAACCTC CCTCTGCCAG AGTACACGAA GCGAGATGGA 
AGGCTGAATT TGGCTTCTAG ACTACCCAGC TATTTTGTAC GACCCGATTT GGGTCCCAAG 
ATGTACAATG CCTATGGTTT AATCACTGCA GAAGATAGAA GAGTTGGTAC AACAAACTTG 
CACTTAGATG TATCTGATGC AGTTAATGTG ATGGTGTATG TTGGAATCCC CATTGGGGAG 
GGAGCTCATG ATGAAGAGGT GCTAAAGACT ATTGATGAGG GTGATGCTGA TGAGGTAACA 
AAACAGCGGA TTCATGAAGG GAATGAGAAA CCTGGAGCGT TATGGCATAT CTATGCAGCT 
AAGGATGCTG AGAAGATCCG AGAACTTCTC CGAAAGGTTG GAGAAGAGCA AGGTCAAGAA 
AATCCTCCAG ATCATGATCC AATCCATGAC CAAAGCTGGT ATCTGGACCA GACTCTTCGT 
AAACGTCTCT ATGAGGAGTA TGGCGTGCAA GGCTGGGCAA TTGTGCAGTT TCTGGGAGAT 
GCTGTCTTTA TACCTGCAGG AGCCCCTCAC CAAGTTCATA ATCTGTACAG TTGCATAAAA 
GTGGCAGAAG ATTTTGTATC TCCTGAACAT GTAAAGCACT GTTTCCGCTT GACCCAGGAG 
TTCAGACATC TGTCTAACAC CCACACAAAT CATGAGGATA AACTGCAGGT AAAGAATATC 
ATTTACCATG CAGTGAAGGA TGCTGTTGGC ACACTGAAGG CTCATGAATC CAAACTGGCA 
AGATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012406142.1
CDS	26..5134
Translation	MRPPGQRLNLGTGIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPFLLQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEFLLDRDLRFLSDATGLRLFQMGTESQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVKGQRVKVYQPEGEEGWICGVVSRQDSLTRLMEVSLSESGEIKSVDPRLIHVMLMDNSASQNEGSTLKAVKPPKGKKKRESIEGKDGRRRKSASDSGCDPATKKLKGERSEVDSNGSDGSETSRGPWKGSSSGEPGLDQRAKQLPPFVPQINRNIRFATYTKENGRTLVVQDEPLGGDMPVPFTPYSSATGQSPLVTEVGGAGNKEAGKSLDSGAQSLAVSTAVITTTSSTPTTVRISDTGLSAVTLQEKQKVNWSQTQGENSRNPLLASSGFGASLSSSSQSLAFGSVRAQSNGVLATESKPLGFSFASSSATETQKDSDLSKNLFFQCMSQTLPSSNYFTAISESLSDESSARESFKQSLESLSSGLCKGKPTLAADTKAGSALDRKMPAESMPTLTPAFPRSLLNTRAPENHENLFLQPPKLSREEPSNPFLAFAEKADLSPFSSFTSQTAGGSTSATTGALKAASSWPESHTSADSASLAKKKSLFITTDSSKLGSSAPSSAVPPGVPSLSAMGNGRSNSPTSSLSQPIEMPTLSSSPTEEKPSVGPGQQDNPLLKTFTNVFGRHPTGFLSSTEFLHDNKAPFEAVKRFSLDERSMTSKQDSDSSTNSDLSDLSDSEEQLQAKAGLKGIPEHLMGKLGPNGERNAELLLGKGRGKQAPKGRPRTAPLKVGQSVLKDMSKVRKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGILRVEGFLSPQQSDPEAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEIGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGISQLPSINPSASSSGSETPFSSGGGTTGVTTPESDLLLKPDTLDSRGEEALKTDASSSSSETKTSRPLCPETTPPSSALHWLADLATQKAKEETKEAGSLRSVLSKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPAASNNKTEGSSLRDLLHSGPGKLPQAPLDTSIPFPPVFSASSTGVKNKASLPNFLDHIIASVVENKKTSDAAKRASNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHEGNEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_012550688.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii lysine (K)-specific demethylase 3B (KDM3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012550688.1

ORF Insert Sequence:

ATGAGGCCTC CAGGCCAGAG ACTAAACCTG GGAACTGGGA TATTTGTGGA ATTTGATGGC 
TGCAACTGGA AGCAACACTC CTGGGTGAAA GTCCATGCTG AAGAGGTTAT TGTGCTGCTC 
CTAGAAGGCT CTTTAGTTTG GGCTCCTCGA AAGGATCCAT TCCTCCTCCA GGGGACCAGG 
GTCTCAATTG CACAGTGGCC AGCCCTGACC TTCACTCCAT TAGTGGATAA ATTGGGTTTG 
GGGTCTGTGG TTCCAGTGGA ATTTCTTCTG GATCGAGATC TCCGATTCTT GTCAGATGCT 
ACTGGATTAC GCCTATTCCA GATGGGAACA GAGAGCCAGA ACCAGATTCT TCTAGAACAT 
GCTGCATTGA GAGATACAGT TAATGCTTTG ATCAGTGACC AAAAGCTCCA GGAAATATTC 
AGTCGAGGTC CCTATAGTGT CAAAGGCCAG AGGGTCAAAG TGTACCAGCC AGAAGGTGAA 
GAAGGTTGGA TCTGTGGTGT TGTAAGCCGA CAAGACTCTC TCACTCGTCT TATGGAGGTA 
TCTTTGTCAG AGAGTGGTGA GATTAAATCA GTGGATCCCA GATTGATACA TGTGATGTTG 
ATGGACAACT CTGCTTCTCA GAATGAGGGA AGCACATTAA AAGCTGTAAA ACCCCCCAAA 
GGAAAGAAGA AAAGAGAGAG CATAGAGGGA AAAGATGGAC GAAGGAGGAA AAGTGCTTCA 
GACTCTGGGT GTGACCCTGC AACAAAGAAA TTAAAAGGAG AAAGGAGTGA AGTAGACAGT 
AATGGGAGTG ATGGAAGTGA AACAAGTCGA GGGCCCTGGA AAGGGAGTTC TAGTGGAGAG 
CCAGGGCTGG ATCAAAGGGC CAAGCAGCTG CCTCCATTTG TTCCCCAGAT CAATCGAAAC 
ATTCGATTTG CCACATACAC CAAAGAGAAT GGCAGGACTC TGGTGGTTCA GGATGAGCCT 
CTAGGTGGGG ACATGCCTGT ACCTTTCACT CCATATTCTT CAGCCACGGG GCAGTCACCT 
TTGGTTACAG AGGTGGGTGG AGCTGGAAAC AAGGAGGCTG GAAAATCACT GGATTCAGGT 
GCTCAGAGTC TGGCAGTTTC AACAGCCGTG ATCACAACCA CCAGCTCAAC ACCAACCACC 
GTGAGAATCT CTGATACAGG CCTTTCAGCA GTGACCCTGC AGGAGAAACA AAAAGTCAAC 
TGGTCTCAGA CTCAGGGAGA GAATTCCAGA AACCCTCTCT TGGCTTCTTC TGGATTTGGA 
GCATCTCTCT CAAGTTCTTC CCAATCCTTG GCTTTTGGAA GTGTTAGAGC CCAGTCCAAT 
GGGGTCTTGG CTACAGAGAG CAAGCCTTTG GGGTTTTCTT TTGCTTCCAG TTCTGCTACA 
GAAACCCAGA AAGATTCTGA TCTTTCAAAG AACTTGTTTT TTCAGTGCAT GTCACAGACT 
TTGCCCTCAA GTAACTACTT CACAGCTATT TCAGAGAGTT TATCTGATGA ATCCTCTGCT 
CGGGAGTCAT TCAAACAGAG CCTAGAGAGC CTGAGCTCTG GGCTTTGTAA AGGCAAACCT 
ACCCTGGCAG CAGATACGAA GGCTGGCAGT GCCCTGGATC GAAAGATGCC TGCAGAGTCC 
ATGCCTACCC TCACTCCAGC CTTCCCAAGG AGCCTCTTGA ATACCCGAGC CCCAGAGAAT 
CATGAAAATC TATTTTTGCA GCCCCCTAAA TTGTCCCGTG AAGAGCCATC CAACCCTTTC 
CTGGCATTTG CAGAGAAAGC TGATCTCAGT CCTTTCAGCA GTTTTACATC TCAGACAGCA 
GGTGGCTCCA CTTCTGCTAC CACTGGAGCA CTCAAAGCAG CTTCTAGCTG GCCAGAGTCT 
CACACCTCTG CTGATTCAGC ATCTTTAGCA AAGAAGAAAT CTCTCTTCAT CACAACTGAT 
TCTTCCAAGC TAGGCTCTAG TGCACCTAGC TCAGCTGTGC CTCCTGGTGT CCCAAGCCTC 
TCTGCCATGG GAAATGGCCG CTCTAATTCA CCCACCAGCA GTCTTTCACA ACCTATTGAG 
ATGCCCACCC TCTCCTCCAG CCCAACAGAG GAAAAGCCAT CAGTTGGACC TGGGCAACAG 
GACAATCCCC TTTTGAAAAC CTTTACTAAT GTCTTTGGCA GGCACCCAAC TGGCTTTCTC 
TCCTCAACAG AGTTTTTACA TGATAACAAA GCTCCTTTTG AAGCTGTAAA AAGGTTTTCA 
CTGGATGAGC GAAGTATGAC ATCTAAACAG GACTCAGATT CCAGCACCAA TAGTGACCTG 
TCAGACCTGA GTGACTCTGA GGAGCAGCTG CAAGCCAAGG CTGGGTTGAA GGGAATCCCC 
GAGCACCTAA TGGGAAAGCT GGGCCCCAAT GGAGAGCGAA ATGCTGAACT ATTACTGGGA 
AAAGGAAGAG GGAAGCAGGC TCCTAAAGGC CGACCTCGAA CAGCCCCTCT GAAAGTTGGC 
CAATCTGTGC TAAAAGACAT GAGCAAGGTA AGAAAGCTCA AACAGTCAGG AGAGCCTTTC 
CTCCAAGATG GATCCTGCAT TAATGTGGCC CCTCACCTCC ATAAGTGCAG GGAGTGCCGC 
CTGGAGCGCT ACCGAAAGTT TAAAGAGCAA GAACAAGATG ATTCCACTGT GGCCTGCCGA 
TTCTTTCACT TCCGCAGGCT TATCTTCACT CGGAAAGGCA TCCTCCGTGT AGAAGGGTTT 
TTGAGTCCGC AGCAAAGTGA TCCAGAAGCC ATGAATCTGT GGATTCCCTC TTCCTCCCTT 
GCAGAAGGAA TCGATCTGGA GACCTCAAAA TACATACTAG CCAATGTCGG GGACCAGTTT 
TGCCAGCTTG TCATGTCAGA AAAGGAGGCC ATGATGATGG TAGAGCCACA CCAAAAAGTG 
GCATGGAAAC GAGCTGTACG TGGTGTCAGG GAAATGTGTG ATGTATGTGA AACAACTCTC 
TTCAACATCC ATTGGGTTTG TCGCAAATGT GGATTTGGAG TCTGTCTGGA CTGTTACCGA 
CTTAGAAAGA GTCGTCCACG TAGTGAGACC GAAGAGATTG GTGATGAAGA GGTTTTCTCT 
TGGTTGAAAT GTGCAAAGGG GCAGTCACAT GAACCAGAAA ATCTCATGCC TACACAAATC 
ATCCCTGGCA CAGCTCTTTA CAATATTGGA GATATGGTAC ATGCAGCCCG GGGCAAGTGG 
GGAATTAAAG CCAATTGTCC TTGCATTAGT CGTCAGAATA AATCTGTATT GAGGCCTGCT 
GTCACTAATG GGATTTCACA GCTTCCCAGT ATAAATCCCA GTGCTTCCTC TTCTGGAAGT 
GAAACTCCCT TCTCCAGTGG AGGAGGGACA ACAGGGGTGA CAACACCAGA GTCTGACCTA 
TTACTCAAAC CTGACACTCT GGACAGCAGG GGTGAGGAGG CACTGAAAAC TGATGCATCA 
AGTAGCAGTA GTGAGACAAA AACCAGCAGG CCACTGTGCC CCGAAACAAC ACCACCATCC 
TCTGCTTTGC ACTGGCTGGC AGATTTAGCA ACACAGAAAG CAAAGGAAGA AACAAAAGAA 
GCTGGATCCC TGAGATCAGT ACTCAGTAAG GAGTCTCATT CCCCCTTTGG GCTGGATTCC 
TTCAACTCTA CAGCAAAGGT TTCTCCATTA ACTCCAAAGC TCTTTAATAG TCTGCTACTG 
GGTCCTGCTG CCTCAAACAA CAAAACAGAA GGCTCCAGCC TTCGAGACCT ACTACACTCT 
GGACCCGGAA AGCTTCCCCA GGCCCCCTTG GACACCAGCA TACCTTTCCC TCCAGTCTTC 
TCAGCATCTT CAACAGGCGT GAAGAATAAG GCCAGTCTTC CCAACTTCCT CGACCACATC 
ATTGCCTCAG TGGTAGAAAA TAAAAAAACT TCTGATGCTG CAAAACGGGC CAGTAACTTG 
ACAGATACCC AAAAAGAAGT GAAGGAGATG GTAATGGGGC TGAATGTGTT GGATCCACAC 
ACCTCCCACT CATGGCTCTG TGATGGGAGG CTCCTATGTC TCCATGACCC CAGCAACAAA 
AACAACTGGA AGATCTTTCG GGAGTGTTGG AAGCAAGGCC AGCCTGTCCT TGTTTCCGGG 
GTGCATAAGA AGCTCAAATC TGAACTTTGG AAGCCAGAAG CATTTAGTCA AGAATTTGGA 
GACCAGGATG TGGACTTAGT AAACTGTAGG AACTGTGCTA TCATTTCTGA TGTGAAAGTG 
CGTGACTTCT GGGATGGTTT CGAAATCATA TGCAAACGGC TAAGGTCAGA AGATGGGCAG 
CCAATGGTAC TGAAACTCAA AGACTGGCCT CCAGGGGAAG ATTTCCGAGA CATGATGCCT 
ACAAGGTTTG AGGATCTGAT GGAGAACCTC CCTCTGCCAG AGTACACGAA GCGAGATGGA 
AGGCTGAATT TGGCTTCTAG ACTACCCAGC TATTTTGTAC GACCCGATTT GGGTCCCAAG 
ATGTACAATG CCTATGGTTT AATCACTGCA GAAGATAGAA GAGTTGGTAC AACAAACTTG 
CACTTAGATG TATCTGATGC AGTTAATGTG ATGGTGTATG TTGGAATCCC CATTGGGGAG 
GGAGCTCATG ATGAAGAGGT GCTAAAGACT ATTGATGAGG GTGATGCTGA TGAGGTAACA 
AAACAGCGGA TTCATGAAGG GAATGAGAAA CCTGGAGCGT TATGGCATAT CTATGCAGCT 
AAGGATGCTG AGAAGATCCG AGAACTTCTC CGAAAGGTTG GAGAAGAGCA AGGTCAAGAA 
AATCCTCCAG ATCATGATCC AATCCATGAC CAAAGCTGGT ATCTGGACCA GACTCTTCGT 
AAACGTCTCT ATGAGGAGTA TGGCGTGCAA GGCTGGGCAA TTGTGCAGTT TCTGGGAGAT 
GCTGTCTTTA TACCTGCAGG AGCCCCTCAC CAAGTTCATA ATCTGTACAG TTGCATAAAA 
GTGGCAGAAG ATTTTGTATC TCCTGAACAT GTAAAGCACT GTTTCCGCTT GACCCAGGAG 
TTCAGACATC TGTCTAACAC CCACACAAAT CATGAGGATA AACTGCAGGT AAAGAATATC 
ATTTACCATG CAGTGAAGGA TGCTGTTGGC ACACTGAAGG CTCATGAATC CAAACTGGCA 
AGATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_012550688.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012406142.1 Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X1, mRNA.

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CloneID	OSa118100
Clone ID Related Accession (Same CDS sequence)	XM_012550688.1 , XM_012550688.2
Accession Version	XM_012550688.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	lysine-specific demethylase 3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816604.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012550688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCTC CAGGCCAGAG ACTAAACCTG GGAACTGGGA TATTTGTGGA ATTTGATGGC 
TGCAACTGGA AGCAACACTC CTGGGTGAAA GTCCATGCTG AAGAGGTTAT TGTGCTGCTC 
CTAGAAGGCT CTTTAGTTTG GGCTCCTCGA AAGGATCCAT TCCTCCTCCA GGGGACCAGG 
GTCTCAATTG CACAGTGGCC AGCCCTGACC TTCACTCCAT TAGTGGATAA ATTGGGTTTG 
GGGTCTGTGG TTCCAGTGGA ATTTCTTCTG GATCGAGATC TCCGATTCTT GTCAGATGCT 
ACTGGATTAC GCCTATTCCA GATGGGAACA GAGAGCCAGA ACCAGATTCT TCTAGAACAT 
GCTGCATTGA GAGATACAGT TAATGCTTTG ATCAGTGACC AAAAGCTCCA GGAAATATTC 
AGTCGAGGTC CCTATAGTGT CAAAGGCCAG AGGGTCAAAG TGTACCAGCC AGAAGGTGAA 
GAAGGTTGGA TCTGTGGTGT TGTAAGCCGA CAAGACTCTC TCACTCGTCT TATGGAGGTA 
TCTTTGTCAG AGAGTGGTGA GATTAAATCA GTGGATCCCA GATTGATACA TGTGATGTTG 
ATGGACAACT CTGCTTCTCA GAATGAGGGA AGCACATTAA AAGCTGTAAA ACCCCCCAAA 
GGAAAGAAGA AAAGAGAGAG CATAGAGGGA AAAGATGGAC GAAGGAGGAA AAGTGCTTCA 
GACTCTGGGT GTGACCCTGC AACAAAGAAA TTAAAAGGAG AAAGGAGTGA AGTAGACAGT 
AATGGGAGTG ATGGAAGTGA AACAAGTCGA GGGCCCTGGA AAGGGAGTTC TAGTGGAGAG 
CCAGGGCTGG ATCAAAGGGC CAAGCAGCTG CCTCCATTTG TTCCCCAGAT CAATCGAAAC 
ATTCGATTTG CCACATACAC CAAAGAGAAT GGCAGGACTC TGGTGGTTCA GGATGAGCCT 
CTAGGTGGGG ACATGCCTGT ACCTTTCACT CCATATTCTT CAGCCACGGG GCAGTCACCT 
TTGGTTACAG AGGTGGGTGG AGCTGGAAAC AAGGAGGCTG GAAAATCACT GGATTCAGGT 
GCTCAGAGTC TGGCAGTTTC AACAGCCGTG ATCACAACCA CCAGCTCAAC ACCAACCACC 
GTGAGAATCT CTGATACAGG CCTTTCAGCA GTGACCCTGC AGGAGAAACA AAAAGTCAAC 
TGGTCTCAGA CTCAGGGAGA GAATTCCAGA AACCCTCTCT TGGCTTCTTC TGGATTTGGA 
GCATCTCTCT CAAGTTCTTC CCAATCCTTG GCTTTTGGAA GTGTTAGAGC CCAGTCCAAT 
GGGGTCTTGG CTACAGAGAG CAAGCCTTTG GGGTTTTCTT TTGCTTCCAG TTCTGCTACA 
GAAACCCAGA AAGATTCTGA TCTTTCAAAG AACTTGTTTT TTCAGTGCAT GTCACAGACT 
TTGCCCTCAA GTAACTACTT CACAGCTATT TCAGAGAGTT TATCTGATGA ATCCTCTGCT 
CGGGAGTCAT TCAAACAGAG CCTAGAGAGC CTGAGCTCTG GGCTTTGTAA AGGCAAACCT 
ACCCTGGCAG CAGATACGAA GGCTGGCAGT GCCCTGGATC GAAAGATGCC TGCAGAGTCC 
ATGCCTACCC TCACTCCAGC CTTCCCAAGG AGCCTCTTGA ATACCCGAGC CCCAGAGAAT 
CATGAAAATC TATTTTTGCA GCCCCCTAAA TTGTCCCGTG AAGAGCCATC CAACCCTTTC 
CTGGCATTTG CAGAGAAAGC TGATCTCAGT CCTTTCAGCA GTTTTACATC TCAGACAGCA 
GGTGGCTCCA CTTCTGCTAC CACTGGAGCA CTCAAAGCAG CTTCTAGCTG GCCAGAGTCT 
CACACCTCTG CTGATTCAGC ATCTTTAGCA AAGAAGAAAT CTCTCTTCAT CACAACTGAT 
TCTTCCAAGC TAGGCTCTAG TGCACCTAGC TCAGCTGTGC CTCCTGGTGT CCCAAGCCTC 
TCTGCCATGG GAAATGGCCG CTCTAATTCA CCCACCAGCA GTCTTTCACA ACCTATTGAG 
ATGCCCACCC TCTCCTCCAG CCCAACAGAG GAAAAGCCAT CAGTTGGACC TGGGCAACAG 
GACAATCCCC TTTTGAAAAC CTTTACTAAT GTCTTTGGCA GGCACCCAAC TGGCTTTCTC 
TCCTCAACAG AGTTTTTACA TGATAACAAA GCTCCTTTTG AAGCTGTAAA AAGGTTTTCA 
CTGGATGAGC GAAGTATGAC ATCTAAACAG GACTCAGATT CCAGCACCAA TAGTGACCTG 
TCAGACCTGA GTGACTCTGA GGAGCAGCTG CAAGCCAAGG CTGGGTTGAA GGGAATCCCC 
GAGCACCTAA TGGGAAAGCT GGGCCCCAAT GGAGAGCGAA ATGCTGAACT ATTACTGGGA 
AAAGGAAGAG GGAAGCAGGC TCCTAAAGGC CGACCTCGAA CAGCCCCTCT GAAAGTTGGC 
CAATCTGTGC TAAAAGACAT GAGCAAGGTA AGAAAGCTCA AACAGTCAGG AGAGCCTTTC 
CTCCAAGATG GATCCTGCAT TAATGTGGCC CCTCACCTCC ATAAGTGCAG GGAGTGCCGC 
CTGGAGCGCT ACCGAAAGTT TAAAGAGCAA GAACAAGATG ATTCCACTGT GGCCTGCCGA 
TTCTTTCACT TCCGCAGGCT TATCTTCACT CGGAAAGGCA TCCTCCGTGT AGAAGGGTTT 
TTGAGTCCGC AGCAAAGTGA TCCAGAAGCC ATGAATCTGT GGATTCCCTC TTCCTCCCTT 
GCAGAAGGAA TCGATCTGGA GACCTCAAAA TACATACTAG CCAATGTCGG GGACCAGTTT 
TGCCAGCTTG TCATGTCAGA AAAGGAGGCC ATGATGATGG TAGAGCCACA CCAAAAAGTG 
GCATGGAAAC GAGCTGTACG TGGTGTCAGG GAAATGTGTG ATGTATGTGA AACAACTCTC 
TTCAACATCC ATTGGGTTTG TCGCAAATGT GGATTTGGAG TCTGTCTGGA CTGTTACCGA 
CTTAGAAAGA GTCGTCCACG TAGTGAGACC GAAGAGATTG GTGATGAAGA GGTTTTCTCT 
TGGTTGAAAT GTGCAAAGGG GCAGTCACAT GAACCAGAAA ATCTCATGCC TACACAAATC 
ATCCCTGGCA CAGCTCTTTA CAATATTGGA GATATGGTAC ATGCAGCCCG GGGCAAGTGG 
GGAATTAAAG CCAATTGTCC TTGCATTAGT CGTCAGAATA AATCTGTATT GAGGCCTGCT 
GTCACTAATG GGATTTCACA GCTTCCCAGT ATAAATCCCA GTGCTTCCTC TTCTGGAAGT 
GAAACTCCCT TCTCCAGTGG AGGAGGGACA ACAGGGGTGA CAACACCAGA GTCTGACCTA 
TTACTCAAAC CTGACACTCT GGACAGCAGG GGTGAGGAGG CACTGAAAAC TGATGCATCA 
AGTAGCAGTA GTGAGACAAA AACCAGCAGG CCACTGTGCC CCGAAACAAC ACCACCATCC 
TCTGCTTTGC ACTGGCTGGC AGATTTAGCA ACACAGAAAG CAAAGGAAGA AACAAAAGAA 
GCTGGATCCC TGAGATCAGT ACTCAGTAAG GAGTCTCATT CCCCCTTTGG GCTGGATTCC 
TTCAACTCTA CAGCAAAGGT TTCTCCATTA ACTCCAAAGC TCTTTAATAG TCTGCTACTG 
GGTCCTGCTG CCTCAAACAA CAAAACAGAA GGCTCCAGCC TTCGAGACCT ACTACACTCT 
GGACCCGGAA AGCTTCCCCA GGCCCCCTTG GACACCAGCA TACCTTTCCC TCCAGTCTTC 
TCAGCATCTT CAACAGGCGT GAAGAATAAG GCCAGTCTTC CCAACTTCCT CGACCACATC 
ATTGCCTCAG TGGTAGAAAA TAAAAAAACT TCTGATGCTG CAAAACGGGC CAGTAACTTG 
ACAGATACCC AAAAAGAAGT GAAGGAGATG GTAATGGGGC TGAATGTGTT GGATCCACAC 
ACCTCCCACT CATGGCTCTG TGATGGGAGG CTCCTATGTC TCCATGACCC CAGCAACAAA 
AACAACTGGA AGATCTTTCG GGAGTGTTGG AAGCAAGGCC AGCCTGTCCT TGTTTCCGGG 
GTGCATAAGA AGCTCAAATC TGAACTTTGG AAGCCAGAAG CATTTAGTCA AGAATTTGGA 
GACCAGGATG TGGACTTAGT AAACTGTAGG AACTGTGCTA TCATTTCTGA TGTGAAAGTG 
CGTGACTTCT GGGATGGTTT CGAAATCATA TGCAAACGGC TAAGGTCAGA AGATGGGCAG 
CCAATGGTAC TGAAACTCAA AGACTGGCCT CCAGGGGAAG ATTTCCGAGA CATGATGCCT 
ACAAGGTTTG AGGATCTGAT GGAGAACCTC CCTCTGCCAG AGTACACGAA GCGAGATGGA 
AGGCTGAATT TGGCTTCTAG ACTACCCAGC TATTTTGTAC GACCCGATTT GGGTCCCAAG 
ATGTACAATG CCTATGGTTT AATCACTGCA GAAGATAGAA GAGTTGGTAC AACAAACTTG 
CACTTAGATG TATCTGATGC AGTTAATGTG ATGGTGTATG TTGGAATCCC CATTGGGGAG 
GGAGCTCATG ATGAAGAGGT GCTAAAGACT ATTGATGAGG GTGATGCTGA TGAGGTAACA 
AAACAGCGGA TTCATGAAGG GAATGAGAAA CCTGGAGCGT TATGGCATAT CTATGCAGCT 
AAGGATGCTG AGAAGATCCG AGAACTTCTC CGAAAGGTTG GAGAAGAGCA AGGTCAAGAA 
AATCCTCCAG ATCATGATCC AATCCATGAC CAAAGCTGGT ATCTGGACCA GACTCTTCGT 
AAACGTCTCT ATGAGGAGTA TGGCGTGCAA GGCTGGGCAA TTGTGCAGTT TCTGGGAGAT 
GCTGTCTTTA TACCTGCAGG AGCCCCTCAC CAAGTTCATA ATCTGTACAG TTGCATAAAA 
GTGGCAGAAG ATTTTGTATC TCCTGAACAT GTAAAGCACT GTTTCCGCTT GACCCAGGAG 
TTCAGACATC TGTCTAACAC CCACACAAAT CATGAGGATA AACTGCAGGT AAAGAATATC 
ATTTACCATG CAGTGAAGGA TGCTGTTGGC ACACTGAAGG CTCATGAATC CAAACTGGCA 
AGATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012406142.1
CDS	28..5136
Translation	MRPPGQRLNLGTGIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPFLLQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEFLLDRDLRFLSDATGLRLFQMGTESQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVKGQRVKVYQPEGEEGWICGVVSRQDSLTRLMEVSLSESGEIKSVDPRLIHVMLMDNSASQNEGSTLKAVKPPKGKKKRESIEGKDGRRRKSASDSGCDPATKKLKGERSEVDSNGSDGSETSRGPWKGSSSGEPGLDQRAKQLPPFVPQINRNIRFATYTKENGRTLVVQDEPLGGDMPVPFTPYSSATGQSPLVTEVGGAGNKEAGKSLDSGAQSLAVSTAVITTTSSTPTTVRISDTGLSAVTLQEKQKVNWSQTQGENSRNPLLASSGFGASLSSSSQSLAFGSVRAQSNGVLATESKPLGFSFASSSATETQKDSDLSKNLFFQCMSQTLPSSNYFTAISESLSDESSARESFKQSLESLSSGLCKGKPTLAADTKAGSALDRKMPAESMPTLTPAFPRSLLNTRAPENHENLFLQPPKLSREEPSNPFLAFAEKADLSPFSSFTSQTAGGSTSATTGALKAASSWPESHTSADSASLAKKKSLFITTDSSKLGSSAPSSAVPPGVPSLSAMGNGRSNSPTSSLSQPIEMPTLSSSPTEEKPSVGPGQQDNPLLKTFTNVFGRHPTGFLSSTEFLHDNKAPFEAVKRFSLDERSMTSKQDSDSSTNSDLSDLSDSEEQLQAKAGLKGIPEHLMGKLGPNGERNAELLLGKGRGKQAPKGRPRTAPLKVGQSVLKDMSKVRKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGILRVEGFLSPQQSDPEAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEIGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGISQLPSINPSASSSGSETPFSSGGGTTGVTTPESDLLLKPDTLDSRGEEALKTDASSSSSETKTSRPLCPETTPPSSALHWLADLATQKAKEETKEAGSLRSVLSKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPAASNNKTEGSSLRDLLHSGPGKLPQAPLDTSIPFPPVFSASSTGVKNKASLPNFLDHIIASVVENKKTSDAAKRASNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHEGNEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_012550688.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012550688.2

ORF Insert Sequence:

ATGAGGCCTC CAGGCCAGAG ACTAAACCTG GGAACTGGGA TATTTGTGGA ATTTGATGGC 
TGCAACTGGA AGCAACACTC CTGGGTGAAA GTCCATGCTG AAGAGGTTAT TGTGCTGCTC 
CTAGAAGGCT CTTTAGTTTG GGCTCCTCGA AAGGATCCAT TCCTCCTCCA GGGGACCAGG 
GTCTCAATTG CACAGTGGCC AGCCCTGACC TTCACTCCAT TAGTGGATAA ATTGGGTTTG 
GGGTCTGTGG TTCCAGTGGA ATTTCTTCTG GATCGAGATC TCCGATTCTT GTCAGATGCT 
ACTGGATTAC GCCTATTCCA GATGGGAACA GAGAGCCAGA ACCAGATTCT TCTAGAACAT 
GCTGCATTGA GAGATACAGT TAATGCTTTG ATCAGTGACC AAAAGCTCCA GGAAATATTC 
AGTCGAGGTC CCTATAGTGT CAAAGGCCAG AGGGTCAAAG TGTACCAGCC AGAAGGTGAA 
GAAGGTTGGA TCTGTGGTGT TGTAAGCCGA CAAGACTCTC TCACTCGTCT TATGGAGGTA 
TCTTTGTCAG AGAGTGGTGA GATTAAATCA GTGGATCCCA GATTGATACA TGTGATGTTG 
ATGGACAACT CTGCTTCTCA GAATGAGGGA AGCACATTAA AAGCTGTAAA ACCCCCCAAA 
GGAAAGAAGA AAAGAGAGAG CATAGAGGGA AAAGATGGAC GAAGGAGGAA AAGTGCTTCA 
GACTCTGGGT GTGACCCTGC AACAAAGAAA TTAAAAGGAG AAAGGAGTGA AGTAGACAGT 
AATGGGAGTG ATGGAAGTGA AACAAGTCGA GGGCCCTGGA AAGGGAGTTC TAGTGGAGAG 
CCAGGGCTGG ATCAAAGGGC CAAGCAGCTG CCTCCATTTG TTCCCCAGAT CAATCGAAAC 
ATTCGATTTG CCACATACAC CAAAGAGAAT GGCAGGACTC TGGTGGTTCA GGATGAGCCT 
CTAGGTGGGG ACATGCCTGT ACCTTTCACT CCATATTCTT CAGCCACGGG GCAGTCACCT 
TTGGTTACAG AGGTGGGTGG AGCTGGAAAC AAGGAGGCTG GAAAATCACT GGATTCAGGT 
GCTCAGAGTC TGGCAGTTTC AACAGCCGTG ATCACAACCA CCAGCTCAAC ACCAACCACC 
GTGAGAATCT CTGATACAGG CCTTTCAGCA GTGACCCTGC AGGAGAAACA AAAAGTCAAC 
TGGTCTCAGA CTCAGGGAGA GAATTCCAGA AACCCTCTCT TGGCTTCTTC TGGATTTGGA 
GCATCTCTCT CAAGTTCTTC CCAATCCTTG GCTTTTGGAA GTGTTAGAGC CCAGTCCAAT 
GGGGTCTTGG CTACAGAGAG CAAGCCTTTG GGGTTTTCTT TTGCTTCCAG TTCTGCTACA 
GAAACCCAGA AAGATTCTGA TCTTTCAAAG AACTTGTTTT TTCAGTGCAT GTCACAGACT 
TTGCCCTCAA GTAACTACTT CACAGCTATT TCAGAGAGTT TATCTGATGA ATCCTCTGCT 
CGGGAGTCAT TCAAACAGAG CCTAGAGAGC CTGAGCTCTG GGCTTTGTAA AGGCAAACCT 
ACCCTGGCAG CAGATACGAA GGCTGGCAGT GCCCTGGATC GAAAGATGCC TGCAGAGTCC 
ATGCCTACCC TCACTCCAGC CTTCCCAAGG AGCCTCTTGA ATACCCGAGC CCCAGAGAAT 
CATGAAAATC TATTTTTGCA GCCCCCTAAA TTGTCCCGTG AAGAGCCATC CAACCCTTTC 
CTGGCATTTG CAGAGAAAGC TGATCTCAGT CCTTTCAGCA GTTTTACATC TCAGACAGCA 
GGTGGCTCCA CTTCTGCTAC CACTGGAGCA CTCAAAGCAG CTTCTAGCTG GCCAGAGTCT 
CACACCTCTG CTGATTCAGC ATCTTTAGCA AAGAAGAAAT CTCTCTTCAT CACAACTGAT 
TCTTCCAAGC TAGGCTCTAG TGCACCTAGC TCAGCTGTGC CTCCTGGTGT CCCAAGCCTC 
TCTGCCATGG GAAATGGCCG CTCTAATTCA CCCACCAGCA GTCTTTCACA ACCTATTGAG 
ATGCCCACCC TCTCCTCCAG CCCAACAGAG GAAAAGCCAT CAGTTGGACC TGGGCAACAG 
GACAATCCCC TTTTGAAAAC CTTTACTAAT GTCTTTGGCA GGCACCCAAC TGGCTTTCTC 
TCCTCAACAG AGTTTTTACA TGATAACAAA GCTCCTTTTG AAGCTGTAAA AAGGTTTTCA 
CTGGATGAGC GAAGTATGAC ATCTAAACAG GACTCAGATT CCAGCACCAA TAGTGACCTG 
TCAGACCTGA GTGACTCTGA GGAGCAGCTG CAAGCCAAGG CTGGGTTGAA GGGAATCCCC 
GAGCACCTAA TGGGAAAGCT GGGCCCCAAT GGAGAGCGAA ATGCTGAACT ATTACTGGGA 
AAAGGAAGAG GGAAGCAGGC TCCTAAAGGC CGACCTCGAA CAGCCCCTCT GAAAGTTGGC 
CAATCTGTGC TAAAAGACAT GAGCAAGGTA AGAAAGCTCA AACAGTCAGG AGAGCCTTTC 
CTCCAAGATG GATCCTGCAT TAATGTGGCC CCTCACCTCC ATAAGTGCAG GGAGTGCCGC 
CTGGAGCGCT ACCGAAAGTT TAAAGAGCAA GAACAAGATG ATTCCACTGT GGCCTGCCGA 
TTCTTTCACT TCCGCAGGCT TATCTTCACT CGGAAAGGCA TCCTCCGTGT AGAAGGGTTT 
TTGAGTCCGC AGCAAAGTGA TCCAGAAGCC ATGAATCTGT GGATTCCCTC TTCCTCCCTT 
GCAGAAGGAA TCGATCTGGA GACCTCAAAA TACATACTAG CCAATGTCGG GGACCAGTTT 
TGCCAGCTTG TCATGTCAGA AAAGGAGGCC ATGATGATGG TAGAGCCACA CCAAAAAGTG 
GCATGGAAAC GAGCTGTACG TGGTGTCAGG GAAATGTGTG ATGTATGTGA AACAACTCTC 
TTCAACATCC ATTGGGTTTG TCGCAAATGT GGATTTGGAG TCTGTCTGGA CTGTTACCGA 
CTTAGAAAGA GTCGTCCACG TAGTGAGACC GAAGAGATTG GTGATGAAGA GGTTTTCTCT 
TGGTTGAAAT GTGCAAAGGG GCAGTCACAT GAACCAGAAA ATCTCATGCC TACACAAATC 
ATCCCTGGCA CAGCTCTTTA CAATATTGGA GATATGGTAC ATGCAGCCCG GGGCAAGTGG 
GGAATTAAAG CCAATTGTCC TTGCATTAGT CGTCAGAATA AATCTGTATT GAGGCCTGCT 
GTCACTAATG GGATTTCACA GCTTCCCAGT ATAAATCCCA GTGCTTCCTC TTCTGGAAGT 
GAAACTCCCT TCTCCAGTGG AGGAGGGACA ACAGGGGTGA CAACACCAGA GTCTGACCTA 
TTACTCAAAC CTGACACTCT GGACAGCAGG GGTGAGGAGG CACTGAAAAC TGATGCATCA 
AGTAGCAGTA GTGAGACAAA AACCAGCAGG CCACTGTGCC CCGAAACAAC ACCACCATCC 
TCTGCTTTGC ACTGGCTGGC AGATTTAGCA ACACAGAAAG CAAAGGAAGA AACAAAAGAA 
GCTGGATCCC TGAGATCAGT ACTCAGTAAG GAGTCTCATT CCCCCTTTGG GCTGGATTCC 
TTCAACTCTA CAGCAAAGGT TTCTCCATTA ACTCCAAAGC TCTTTAATAG TCTGCTACTG 
GGTCCTGCTG CCTCAAACAA CAAAACAGAA GGCTCCAGCC TTCGAGACCT ACTACACTCT 
GGACCCGGAA AGCTTCCCCA GGCCCCCTTG GACACCAGCA TACCTTTCCC TCCAGTCTTC 
TCAGCATCTT CAACAGGCGT GAAGAATAAG GCCAGTCTTC CCAACTTCCT CGACCACATC 
ATTGCCTCAG TGGTAGAAAA TAAAAAAACT TCTGATGCTG CAAAACGGGC CAGTAACTTG 
ACAGATACCC AAAAAGAAGT GAAGGAGATG GTAATGGGGC TGAATGTGTT GGATCCACAC 
ACCTCCCACT CATGGCTCTG TGATGGGAGG CTCCTATGTC TCCATGACCC CAGCAACAAA 
AACAACTGGA AGATCTTTCG GGAGTGTTGG AAGCAAGGCC AGCCTGTCCT TGTTTCCGGG 
GTGCATAAGA AGCTCAAATC TGAACTTTGG AAGCCAGAAG CATTTAGTCA AGAATTTGGA 
GACCAGGATG TGGACTTAGT AAACTGTAGG AACTGTGCTA TCATTTCTGA TGTGAAAGTG 
CGTGACTTCT GGGATGGTTT CGAAATCATA TGCAAACGGC TAAGGTCAGA AGATGGGCAG 
CCAATGGTAC TGAAACTCAA AGACTGGCCT CCAGGGGAAG ATTTCCGAGA CATGATGCCT 
ACAAGGTTTG AGGATCTGAT GGAGAACCTC CCTCTGCCAG AGTACACGAA GCGAGATGGA 
AGGCTGAATT TGGCTTCTAG ACTACCCAGC TATTTTGTAC GACCCGATTT GGGTCCCAAG 
ATGTACAATG CCTATGGTTT AATCACTGCA GAAGATAGAA GAGTTGGTAC AACAAACTTG 
CACTTAGATG TATCTGATGC AGTTAATGTG ATGGTGTATG TTGGAATCCC CATTGGGGAG 
GGAGCTCATG ATGAAGAGGT GCTAAAGACT ATTGATGAGG GTGATGCTGA TGAGGTAACA 
AAACAGCGGA TTCATGAAGG GAATGAGAAA CCTGGAGCGT TATGGCATAT CTATGCAGCT 
AAGGATGCTG AGAAGATCCG AGAACTTCTC CGAAAGGTTG GAGAAGAGCA AGGTCAAGAA 
AATCCTCCAG ATCATGATCC AATCCATGAC CAAAGCTGGT ATCTGGACCA GACTCTTCGT 
AAACGTCTCT ATGAGGAGTA TGGCGTGCAA GGCTGGGCAA TTGTGCAGTT TCTGGGAGAT 
GCTGTCTTTA TACCTGCAGG AGCCCCTCAC CAAGTTCATA ATCTGTACAG TTGCATAAAA 
GTGGCAGAAG ATTTTGTATC TCCTGAACAT GTAAAGCACT GTTTCCGCTT GACCCAGGAG 
TTCAGACATC TGTCTAACAC CCACACAAAT CATGAGGATA AACTGCAGGT AAAGAATATC 
ATTTACCATG CAGTGAAGGA TGCTGTTGGC ACACTGAAGG CTCATGAATC CAAACTGGCA 
AGATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_023504799.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023360567.1 Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X2, mRNA.

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CloneID	OSa149910
Clone ID Related Accession (Same CDS sequence)	XM_023504799.1
Accession Version	XM_023504799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	lysine-specific demethylase 3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003816604.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGA AATTCCAGAA GATATTTGTG GAATTTGATG GCTGCAACTG GAAGCAACAC 
TCCTGGGTGA AAGTCCATGC TGAAGAGGTT ATTGTGCTGC TCCTAGAAGG CTCTTTAGTT 
TGGGCTCCTC GAAAGGATCC ATTCCTCCTC CAGGGGACCA GGGTCTCAAT TGCACAGTGG 
CCAGCCCTGA CCTTCACTCC ATTAGTGGAT AAATTGGGTT TGGGGTCTGT GGTTCCAGTG 
GAATTTCTTC TGGATCGAGA TCTCCGATTC TTGTCAGATG CTACTGGATT ACGCCTATTC 
CAGATGGGAA CAGAGAGCCA GAACCAGATT CTTCTAGAAC ATGCTGCATT GAGAGATACA 
GTTAATGCTT TGATCAGTGA CCAAAAGCTC CAGGAAATAT TCAGTCGAGG TCCCTATAGT 
GTCAAAGGCC AGAGGGTCAA AGTGTACCAG CCAGAAGGTG AAGAAGGTTG GATCTGTGGT 
GTTGTAAGCC GACAAGACTC TCTCACTCGT CTTATGGAGG TATCTTTGTC AGAGAGTGGT 
GAGATTAAAT CAGTGGATCC CAGATTGATA CATGTGATGT TGATGGACAA CTCTGCTTCT 
CAGAATGAGG GAAGCACATT AAAAGCTGTA AAACCCCCCA AAGGAAAGAA GAAAAGAGAG 
AGCATAGAGG GAAAAGATGG ACGAAGGAGG AAAAGTGCTT CAGACTCTGG GTGTGACCCT 
GCAACAAAGA AATTAAAAGG AGAAAGGAGT GAAGTAGACA GTAATGGGAG TGATGGAAGT 
GAAACAAGTC GAGGGCCCTG GAAAGGGAGT TCTAGTGGAG AGCCAGGGCT GGATCAAAGG 
GCCAAGCAGC TGCCTCCATT TGTTCCCCAG ATCAATCGAA ACATTCGATT TGCCACATAC 
ACCAAAGAGA ATGGCAGGAC TCTGGTGGTT CAGGATGAGC CTCTAGGTGG GGACATGCCT 
GTACCTTTCA CTCCATATTC TTCAGCCACG GGGCAGTCAC CTTTGGTTAC AGAGGTGGGT 
GGAGCTGGAA ACAAGGAGGC TGGAAAATCA CTGGATTCAG GTGCTCAGAG TCTGGCAGTT 
TCAACAGCCG TGATCACAAC CACCAGCTCA ACACCAACCA CCGTGAGAAT CTCTGATACA 
GGCCTTTCAG CAGTGACCCT GCAGGAGAAA CAAAAAGTCA ACTGGTCTCA GACTCAGGGA 
GAGAATTCCA GAAACCCTCT CTTGGCTTCT TCTGGATTTG GAGCATCTCT CTCAAGTTCT 
TCCCAATCCT TGGCTTTTGG AAGTGTTAGA GCCCAGTCCA ATGGGGTCTT GGCTACAGAG 
AGCAAGCCTT TGGGGTTTTC TTTTGCTTCC AGTTCTGCTA CAGAAACCCA GAAAGATTCT 
GATCTTTCAA AGAACTTGTT TTTTCAGTGC ATGTCACAGA CTTTGCCCTC AAGTAACTAC 
TTCACAGCTA TTTCAGAGAG TTTATCTGAT GAATCCTCTG CTCGGGAGTC ATTCAAACAG 
AGCCTAGAGA GCCTGAGCTC TGGGCTTTGT AAAGGCAAAC CTACCCTGGC AGCAGATACG 
AAGGCTGGCA GTGCCCTGGA TCGAAAGATG CCTGCAGAGT CCATGCCTAC CCTCACTCCA 
GCCTTCCCAA GGAGCCTCTT GAATACCCGA GCCCCAGAGA ATCATGAAAA TCTATTTTTG 
CAGCCCCCTA AATTGTCCCG TGAAGAGCCA TCCAACCCTT TCCTGGCATT TGCAGAGAAA 
GCTGATCTCA GTCCTTTCAG CAGTTTTACA TCTCAGACAG CAGGTGGCTC CACTTCTGCT 
ACCACTGGAG CACTCAAAGC AGCTTCTAGC TGGCCAGAGT CTCACACCTC TGCTGATTCA 
GCATCTTTAG CAAAGAAGAA ATCTCTCTTC ATCACAACTG ATTCTTCCAA GCTAGGCTCT 
AGTGCACCTA GCTCAGCTGT GCCTCCTGGT GTCCCAAGCC TCTCTGCCAT GGGAAATGGC 
CGCTCTAATT CACCCACCAG CAGTCTTTCA CAACCTATTG AGATGCCCAC CCTCTCCTCC 
AGCCCAACAG AGGAAAAGCC ATCAGTTGGA CCTGGGCAAC AGGACAATCC CCTTTTGAAA 
ACCTTTACTA ATGTCTTTGG CAGGCACCCA ACTGGCTTTC TCTCCTCAAC AGAGTTTTTA 
CATGATAACA AAGCTCCTTT TGAAGCTGTA AAAAGGTTTT CACTGGATGA GCGAAGTATG 
ACATCTAAAC AGGACTCAGA TTCCAGCACC AATAGTGACC TGTCAGACCT GAGTGACTCT 
GAGGAGCAGC TGCAAGCCAA GGCTGGGTTG AAGGGAATCC CCGAGCACCT AATGGGAAAG 
CTGGGCCCCA ATGGAGAGCG AAATGCTGAA CTATTACTGG GAAAAGGAAG AGGGAAGCAG 
GCTCCTAAAG GCCGACCTCG AACAGCCCCT CTGAAAGTTG GCCAATCTGT GCTAAAAGAC 
ATGAGCAAGG TAAGAAAGCT CAAACAGTCA GGAGAGCCTT TCCTCCAAGA TGGATCCTGC 
ATTAATGTGG CCCCTCACCT CCATAAGTGC AGGGAGTGCC GCCTGGAGCG CTACCGAAAG 
TTTAAAGAGC AAGAACAAGA TGATTCCACT GTGGCCTGCC GATTCTTTCA CTTCCGCAGG 
CTTATCTTCA CTCGGAAAGG CATCCTCCGT GTAGAAGGGT TTTTGAGTCC GCAGCAAAGT 
GATCCAGAAG CCATGAATCT GTGGATTCCC TCTTCCTCCC TTGCAGAAGG AATCGATCTG 
GAGACCTCAA AATACATACT AGCCAATGTC GGGGACCAGT TTTGCCAGCT TGTCATGTCA 
GAAAAGGAGG CCATGATGAT GGTAGAGCCA CACCAAAAAG TGGCATGGAA ACGAGCTGTA 
CGTGGTGTCA GGGAAATGTG TGATGTATGT GAAACAACTC TCTTCAACAT CCATTGGGTT 
TGTCGCAAAT GTGGATTTGG AGTCTGTCTG GACTGTTACC GACTTAGAAA GAGTCGTCCA 
CGTAGTGAGA CCGAAGAGAT TGGTGATGAA GAGGTTTTCT CTTGGTTGAA ATGTGCAAAG 
GGGCAGTCAC ATGAACCAGA AAATCTCATG CCTACACAAA TCATCCCTGG CACAGCTCTT 
TACAATATTG GAGATATGGT ACATGCAGCC CGGGGCAAGT GGGGAATTAA AGCCAATTGT 
CCTTGCATTA GTCGTCAGAA TAAATCTGTA TTGAGGCCTG CTGTCACTAA TGGGATTTCA 
CAGCTTCCCA GTATAAATCC CAGTGCTTCC TCTTCTGGAA GTGAAACTCC CTTCTCCAGT 
GGAGGAGGGA CAACAGGGGT GACAACACCA GAGTCTGACC TATTACTCAA ACCTGACACT 
CTGGACAGCA GGGGTGAGGA GGCACTGAAA ACTGATGCAT CAAGTAGCAG TAGTGAGACA 
AAAACCAGCA GGCCACTGTG CCCCGAAACA ACACCACCAT CCTCTGCTTT GCACTGGCTG 
GCAGATTTAG CAACACAGAA AGCAAAGGAA GAAACAAAAG AAGCTGGATC CCTGAGATCA 
GTACTCAGTA AGGAGTCTCA TTCCCCCTTT GGGCTGGATT CCTTCAACTC TACAGCAAAG 
GTTTCTCCAT TAACTCCAAA GCTCTTTAAT AGTCTGCTAC TGGGTCCTGC TGCCTCAAAC 
AACAAAACAG AAGGCTCCAG CCTTCGAGAC CTACTACACT CTGGACCCGG AAAGCTTCCC 
CAGGCCCCCT TGGACACCAG CATACCTTTC CCTCCAGTCT TCTCAGCATC TTCAACAGGC 
GTGAAGAATA AGGCCAGTCT TCCCAACTTC CTCGACCACA TCATTGCCTC AGTGGTAGAA 
AATAAAAAAA CTTCTGATGC TGCAAAACGG GCCAGTAACT TGACAGATAC CCAAAAAGAA 
GTGAAGGAGA TGGTAATGGG GCTGAATGTG TTGGATCCAC ACACCTCCCA CTCATGGCTC 
TGTGATGGGA GGCTCCTATG TCTCCATGAC CCCAGCAACA AAAACAACTG GAAGATCTTT 
CGGGAGTGTT GGAAGCAAGG CCAGCCTGTC CTTGTTTCCG GGGTGCATAA GAAGCTCAAA 
TCTGAACTTT GGAAGCCAGA AGCATTTAGT CAAGAATTTG GAGACCAGGA TGTGGACTTA 
GTAAACTGTA GGAACTGTGC TATCATTTCT GATGTGAAAG TGCGTGACTT CTGGGATGGT 
TTCGAAATCA TATGCAAACG GCTAAGGTCA GAAGATGGGC AGCCAATGGT ACTGAAACTC 
AAAGACTGGC CTCCAGGGGA AGATTTCCGA GACATGATGC CTACAAGGTT TGAGGATCTG 
ATGGAGAACC TCCCTCTGCC AGAGTACACG AAGCGAGATG GAAGGCTGAA TTTGGCTTCT 
AGACTACCCA GCTATTTTGT ACGACCCGAT TTGGGTCCCA AGATGTACAA TGCCTATGGT 
TTAATCACTG CAGAAGATAG AAGAGTTGGT ACAACAAACT TGCACTTAGA TGTATCTGAT 
GCAGTTAATG TGATGGTGTA TGTTGGAATC CCCATTGGGG AGGGAGCTCA TGATGAAGAG 
GTGCTAAAGA CTATTGATGA GGGTGATGCT GATGAGGTAA CAAAACAGCG GATTCATGAA 
GGGAATGAGA AACCTGGAGC GTTATGGCAT ATCTATGCAG CTAAGGATGC TGAGAAGATC 
CGAGAACTTC TCCGAAAGGT TGGAGAAGAG CAAGGTCAAG AAAATCCTCC AGATCATGAT 
CCAATCCATG ACCAAAGCTG GTATCTGGAC CAGACTCTTC GTAAACGTCT CTATGAGGAG 
TATGGCGTGC AAGGCTGGGC AATTGTGCAG TTTCTGGGAG ATGCTGTCTT TATACCTGCA 
GGAGCCCCTC ACCAAGTTCA TAATCTGTAC AGTTGCATAA AAGTGGCAGA AGATTTTGTA 
TCTCCTGAAC ATGTAAAGCA CTGTTTCCGC TTGACCCAGG AGTTCAGACA TCTGTCTAAC 
ACCCACACAA ATCATGAGGA TAAACTGCAG GTAAAGAATA TCATTTACCA TGCAGTGAAG 
GATGCTGTTG GCACACTGAA GGCTCATGAA TCCAAACTGG CAAGATCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023360567.1
CDS	165..5255
Translation	MKKKFQKIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPFLLQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEFLLDRDLRFLSDATGLRLFQMGTESQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVKGQRVKVYQPEGEEGWICGVVSRQDSLTRLMEVSLSESGEIKSVDPRLIHVMLMDNSASQNEGSTLKAVKPPKGKKKRESIEGKDGRRRKSASDSGCDPATKKLKGERSEVDSNGSDGSETSRGPWKGSSSGEPGLDQRAKQLPPFVPQINRNIRFATYTKENGRTLVVQDEPLGGDMPVPFTPYSSATGQSPLVTEVGGAGNKEAGKSLDSGAQSLAVSTAVITTTSSTPTTVRISDTGLSAVTLQEKQKVNWSQTQGENSRNPLLASSGFGASLSSSSQSLAFGSVRAQSNGVLATESKPLGFSFASSSATETQKDSDLSKNLFFQCMSQTLPSSNYFTAISESLSDESSARESFKQSLESLSSGLCKGKPTLAADTKAGSALDRKMPAESMPTLTPAFPRSLLNTRAPENHENLFLQPPKLSREEPSNPFLAFAEKADLSPFSSFTSQTAGGSTSATTGALKAASSWPESHTSADSASLAKKKSLFITTDSSKLGSSAPSSAVPPGVPSLSAMGNGRSNSPTSSLSQPIEMPTLSSSPTEEKPSVGPGQQDNPLLKTFTNVFGRHPTGFLSSTEFLHDNKAPFEAVKRFSLDERSMTSKQDSDSSTNSDLSDLSDSEEQLQAKAGLKGIPEHLMGKLGPNGERNAELLLGKGRGKQAPKGRPRTAPLKVGQSVLKDMSKVRKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGILRVEGFLSPQQSDPEAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEIGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGISQLPSINPSASSSGSETPFSSGGGTTGVTTPESDLLLKPDTLDSRGEEALKTDASSSSSETKTSRPLCPETTPPSSALHWLADLATQKAKEETKEAGSLRSVLSKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPAASNNKTEGSSLRDLLHSGPGKLPQAPLDTSIPFPPVFSASSTGVKNKASLPNFLDHIIASVVENKKTSDAAKRASNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHEGNEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_023504799.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023504799.1

ORF Insert Sequence:

ATGAAAAAGA AATTCCAGAA GATATTTGTG GAATTTGATG GCTGCAACTG GAAGCAACAC 
TCCTGGGTGA AAGTCCATGC TGAAGAGGTT ATTGTGCTGC TCCTAGAAGG CTCTTTAGTT 
TGGGCTCCTC GAAAGGATCC ATTCCTCCTC CAGGGGACCA GGGTCTCAAT TGCACAGTGG 
CCAGCCCTGA CCTTCACTCC ATTAGTGGAT AAATTGGGTT TGGGGTCTGT GGTTCCAGTG 
GAATTTCTTC TGGATCGAGA TCTCCGATTC TTGTCAGATG CTACTGGATT ACGCCTATTC 
CAGATGGGAA CAGAGAGCCA GAACCAGATT CTTCTAGAAC ATGCTGCATT GAGAGATACA 
GTTAATGCTT TGATCAGTGA CCAAAAGCTC CAGGAAATAT TCAGTCGAGG TCCCTATAGT 
GTCAAAGGCC AGAGGGTCAA AGTGTACCAG CCAGAAGGTG AAGAAGGTTG GATCTGTGGT 
GTTGTAAGCC GACAAGACTC TCTCACTCGT CTTATGGAGG TATCTTTGTC AGAGAGTGGT 
GAGATTAAAT CAGTGGATCC CAGATTGATA CATGTGATGT TGATGGACAA CTCTGCTTCT 
CAGAATGAGG GAAGCACATT AAAAGCTGTA AAACCCCCCA AAGGAAAGAA GAAAAGAGAG 
AGCATAGAGG GAAAAGATGG ACGAAGGAGG AAAAGTGCTT CAGACTCTGG GTGTGACCCT 
GCAACAAAGA AATTAAAAGG AGAAAGGAGT GAAGTAGACA GTAATGGGAG TGATGGAAGT 
GAAACAAGTC GAGGGCCCTG GAAAGGGAGT TCTAGTGGAG AGCCAGGGCT GGATCAAAGG 
GCCAAGCAGC TGCCTCCATT TGTTCCCCAG ATCAATCGAA ACATTCGATT TGCCACATAC 
ACCAAAGAGA ATGGCAGGAC TCTGGTGGTT CAGGATGAGC CTCTAGGTGG GGACATGCCT 
GTACCTTTCA CTCCATATTC TTCAGCCACG GGGCAGTCAC CTTTGGTTAC AGAGGTGGGT 
GGAGCTGGAA ACAAGGAGGC TGGAAAATCA CTGGATTCAG GTGCTCAGAG TCTGGCAGTT 
TCAACAGCCG TGATCACAAC CACCAGCTCA ACACCAACCA CCGTGAGAAT CTCTGATACA 
GGCCTTTCAG CAGTGACCCT GCAGGAGAAA CAAAAAGTCA ACTGGTCTCA GACTCAGGGA 
GAGAATTCCA GAAACCCTCT CTTGGCTTCT TCTGGATTTG GAGCATCTCT CTCAAGTTCT 
TCCCAATCCT TGGCTTTTGG AAGTGTTAGA GCCCAGTCCA ATGGGGTCTT GGCTACAGAG 
AGCAAGCCTT TGGGGTTTTC TTTTGCTTCC AGTTCTGCTA CAGAAACCCA GAAAGATTCT 
GATCTTTCAA AGAACTTGTT TTTTCAGTGC ATGTCACAGA CTTTGCCCTC AAGTAACTAC 
TTCACAGCTA TTTCAGAGAG TTTATCTGAT GAATCCTCTG CTCGGGAGTC ATTCAAACAG 
AGCCTAGAGA GCCTGAGCTC TGGGCTTTGT AAAGGCAAAC CTACCCTGGC AGCAGATACG 
AAGGCTGGCA GTGCCCTGGA TCGAAAGATG CCTGCAGAGT CCATGCCTAC CCTCACTCCA 
GCCTTCCCAA GGAGCCTCTT GAATACCCGA GCCCCAGAGA ATCATGAAAA TCTATTTTTG 
CAGCCCCCTA AATTGTCCCG TGAAGAGCCA TCCAACCCTT TCCTGGCATT TGCAGAGAAA 
GCTGATCTCA GTCCTTTCAG CAGTTTTACA TCTCAGACAG CAGGTGGCTC CACTTCTGCT 
ACCACTGGAG CACTCAAAGC AGCTTCTAGC TGGCCAGAGT CTCACACCTC TGCTGATTCA 
GCATCTTTAG CAAAGAAGAA ATCTCTCTTC ATCACAACTG ATTCTTCCAA GCTAGGCTCT 
AGTGCACCTA GCTCAGCTGT GCCTCCTGGT GTCCCAAGCC TCTCTGCCAT GGGAAATGGC 
CGCTCTAATT CACCCACCAG CAGTCTTTCA CAACCTATTG AGATGCCCAC CCTCTCCTCC 
AGCCCAACAG AGGAAAAGCC ATCAGTTGGA CCTGGGCAAC AGGACAATCC CCTTTTGAAA 
ACCTTTACTA ATGTCTTTGG CAGGCACCCA ACTGGCTTTC TCTCCTCAAC AGAGTTTTTA 
CATGATAACA AAGCTCCTTT TGAAGCTGTA AAAAGGTTTT CACTGGATGA GCGAAGTATG 
ACATCTAAAC AGGACTCAGA TTCCAGCACC AATAGTGACC TGTCAGACCT GAGTGACTCT 
GAGGAGCAGC TGCAAGCCAA GGCTGGGTTG AAGGGAATCC CCGAGCACCT AATGGGAAAG 
CTGGGCCCCA ATGGAGAGCG AAATGCTGAA CTATTACTGG GAAAAGGAAG AGGGAAGCAG 
GCTCCTAAAG GCCGACCTCG AACAGCCCCT CTGAAAGTTG GCCAATCTGT GCTAAAAGAC 
ATGAGCAAGG TAAGAAAGCT CAAACAGTCA GGAGAGCCTT TCCTCCAAGA TGGATCCTGC 
ATTAATGTGG CCCCTCACCT CCATAAGTGC AGGGAGTGCC GCCTGGAGCG CTACCGAAAG 
TTTAAAGAGC AAGAACAAGA TGATTCCACT GTGGCCTGCC GATTCTTTCA CTTCCGCAGG 
CTTATCTTCA CTCGGAAAGG CATCCTCCGT GTAGAAGGGT TTTTGAGTCC GCAGCAAAGT 
GATCCAGAAG CCATGAATCT GTGGATTCCC TCTTCCTCCC TTGCAGAAGG AATCGATCTG 
GAGACCTCAA AATACATACT AGCCAATGTC GGGGACCAGT TTTGCCAGCT TGTCATGTCA 
GAAAAGGAGG CCATGATGAT GGTAGAGCCA CACCAAAAAG TGGCATGGAA ACGAGCTGTA 
CGTGGTGTCA GGGAAATGTG TGATGTATGT GAAACAACTC TCTTCAACAT CCATTGGGTT 
TGTCGCAAAT GTGGATTTGG AGTCTGTCTG GACTGTTACC GACTTAGAAA GAGTCGTCCA 
CGTAGTGAGA CCGAAGAGAT TGGTGATGAA GAGGTTTTCT CTTGGTTGAA ATGTGCAAAG 
GGGCAGTCAC ATGAACCAGA AAATCTCATG CCTACACAAA TCATCCCTGG CACAGCTCTT 
TACAATATTG GAGATATGGT ACATGCAGCC CGGGGCAAGT GGGGAATTAA AGCCAATTGT 
CCTTGCATTA GTCGTCAGAA TAAATCTGTA TTGAGGCCTG CTGTCACTAA TGGGATTTCA 
CAGCTTCCCA GTATAAATCC CAGTGCTTCC TCTTCTGGAA GTGAAACTCC CTTCTCCAGT 
GGAGGAGGGA CAACAGGGGT GACAACACCA GAGTCTGACC TATTACTCAA ACCTGACACT 
CTGGACAGCA GGGGTGAGGA GGCACTGAAA ACTGATGCAT CAAGTAGCAG TAGTGAGACA 
AAAACCAGCA GGCCACTGTG CCCCGAAACA ACACCACCAT CCTCTGCTTT GCACTGGCTG 
GCAGATTTAG CAACACAGAA AGCAAAGGAA GAAACAAAAG AAGCTGGATC CCTGAGATCA 
GTACTCAGTA AGGAGTCTCA TTCCCCCTTT GGGCTGGATT CCTTCAACTC TACAGCAAAG 
GTTTCTCCAT TAACTCCAAA GCTCTTTAAT AGTCTGCTAC TGGGTCCTGC TGCCTCAAAC 
AACAAAACAG AAGGCTCCAG CCTTCGAGAC CTACTACACT CTGGACCCGG AAAGCTTCCC 
CAGGCCCCCT TGGACACCAG CATACCTTTC CCTCCAGTCT TCTCAGCATC TTCAACAGGC 
GTGAAGAATA AGGCCAGTCT TCCCAACTTC CTCGACCACA TCATTGCCTC AGTGGTAGAA 
AATAAAAAAA CTTCTGATGC TGCAAAACGG GCCAGTAACT TGACAGATAC CCAAAAAGAA 
GTGAAGGAGA TGGTAATGGG GCTGAATGTG TTGGATCCAC ACACCTCCCA CTCATGGCTC 
TGTGATGGGA GGCTCCTATG TCTCCATGAC CCCAGCAACA AAAACAACTG GAAGATCTTT 
CGGGAGTGTT GGAAGCAAGG CCAGCCTGTC CTTGTTTCCG GGGTGCATAA GAAGCTCAAA 
TCTGAACTTT GGAAGCCAGA AGCATTTAGT CAAGAATTTG GAGACCAGGA TGTGGACTTA 
GTAAACTGTA GGAACTGTGC TATCATTTCT GATGTGAAAG TGCGTGACTT CTGGGATGGT 
TTCGAAATCA TATGCAAACG GCTAAGGTCA GAAGATGGGC AGCCAATGGT ACTGAAACTC 
AAAGACTGGC CTCCAGGGGA AGATTTCCGA GACATGATGC CTACAAGGTT TGAGGATCTG 
ATGGAGAACC TCCCTCTGCC AGAGTACACG AAGCGAGATG GAAGGCTGAA TTTGGCTTCT 
AGACTACCCA GCTATTTTGT ACGACCCGAT TTGGGTCCCA AGATGTACAA TGCCTATGGT 
TTAATCACTG CAGAAGATAG AAGAGTTGGT ACAACAAACT TGCACTTAGA TGTATCTGAT 
GCAGTTAATG TGATGGTGTA TGTTGGAATC CCCATTGGGG AGGGAGCTCA TGATGAAGAG 
GTGCTAAAGA CTATTGATGA GGGTGATGCT GATGAGGTAA CAAAACAGCG GATTCATGAA 
GGGAATGAGA AACCTGGAGC GTTATGGCAT ATCTATGCAG CTAAGGATGC TGAGAAGATC 
CGAGAACTTC TCCGAAAGGT TGGAGAAGAG CAAGGTCAAG AAAATCCTCC AGATCATGAT 
CCAATCCATG ACCAAAGCTG GTATCTGGAC CAGACTCTTC GTAAACGTCT CTATGAGGAG 
TATGGCGTGC AAGGCTGGGC AATTGTGCAG TTTCTGGGAG ATGCTGTCTT TATACCTGCA 
GGAGCCCCTC ACCAAGTTCA TAATCTGTAC AGTTGCATAA AAGTGGCAGA AGATTTTGTA 
TCTCCTGAAC ATGTAAAGCA CTGTTTCCGC TTGACCCAGG AGTTCAGACA TCTGTCTAAC 
ACCCACACAA ATCATGAGGA TAAACTGCAG GTAAAGAATA TCATTTACCA TGCAGTGAAG 
GATGCTGTTG GCACACTGAA GGCTCATGAA TCCAAACTGG CAAGATCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_031953342.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031809202.1 Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X1, mRNA.

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CloneID	OSa225682
Clone ID Related Accession (Same CDS sequence)	XM_031953342.1
Accession Version	XM_031953342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	lysine-specific demethylase 3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACC ATGACTCCTG CCTCGAGTAC CTTCGTAGAG GGCGCTGTTT ATCAGAGGCC 
AGAGTTGGGG GCAGAAGAAA AAAGGACACC CCACCCCGCC CAGCGTGCAA TTCCCCTCCC 
GACCGCAGGA GGCGCTGCAG CGAGGGGAGA CTTGGGGTGG GGCGCTGTGA GAGGCCCGGG 
GCTGCGGCCC CGCTCCGGAC CGCGGGGGGC GCTGTGAGGA GCCCGGGGCT GCGGCTCCGC 
TCCCGGCCGC GGGGGGCGCT GCACAGCGGC GGTGGTTGGT GGTGGCTTGG GGGTGGGGGG 
GAGGGGGGTA GGGGGTGCGG AGGCCTCGCG GGCGGATCGG GCGCTCAGCG GCGGCAGCTT 
CTGGGCTTGG GGAGGCGGTG GGAGCGGCGG GTCCGGCCGG CCCTGGTCAT GGCGGACGCG 
GCGGCCTCTC CGGTGGGGAA GCGGCTGCTG CTTCTGTTTG CGGATGCGGC GGCGGCCTCG 
GCTCAGGCAC CGGTGGCGGC GGCGGCGGCG GCAGCGGCAG CGGCAGCGGC AGCGGCAGCA 
GAGAGCGGGG ATCCGGGGCC TGCGCTGCGC ACTCGGGCCT GGAGGGCTGG GACAGTGCGG 
GCCATGAGCG GGGCGGTGCC CCAGGATCTA GCGATATTTG TGGAATTTGA TGGCTGCAAC 
TGGAAGCAAC ACTCCTGGGT GAAAGTCCAT GCTGAAGAGG TTATTGTGCT GCTCCTAGAA 
GGCTCTTTAG TTTGGGCTCC TCGAAAGGAT CCATTCCTCC TCCAGGGGAC CAGGGTCTCA 
ATTGCACAGT GGCCAGCCCT GACCTTCACT CCATTAGTGG ATAAATTGGG TTTGGGGTCT 
GTGGTTCCAG TGGAATTTCT TCTGGATCGA GATCTCCGAT TCTTGTCAGA TGCTACTGGA 
TTACGCCTAT TCCAGATGGG AACAGAGAGC CAGAACCAGA TTCTTCTAGA ACATGCTGCA 
TTGAGAGATA CAGTTAATGC TTTGATCAGT GACCAAAAGC TCCAGGAAAT ATTCAGTCGA 
GGTCCCTATA GTGTCAAAGG CCAGAGGGTC AAAGTGTACC AGCCAGAAGG TGAAGAAGGT 
TGGATCTGTG GTGTTGTAAG CCGACAAGAC TCTCTCACTC GTCTTATGGA GGTATCTTTG 
TCAGAGAGTG GTGAGATTAA ATCAGTGGAT CCCAGATTGA TACATGTGAT GTTGATGGAC 
AACTCTGCTT CTCAGAATGA GGGAAGCACA TTAAAAGCTG TAAAACCCCC CAAAGGAAAG 
AAGAAAAGAG AGAGCATAGA GGGAAAAGAT GGACGAAGGA GGAAAAGTGC TTCAGACTCT 
GGGTGTGACC CTGCAACAAA GAAATTAAAA GGAGAAAGGA GTGAAGTAGA CAGTAATGGG 
AGTGATGGAA GTGAAACAAG TCGAGGGCCC TGGAAAGGGA GTTCTAGTGG AGAGCCAGGG 
CTGGATCAAA GGGCCAAGCA GCTGCCTCCA TTTGTTCCCC AGATCAATCG AAACATTCGA 
TTTGCCACAT ACACCAAAGA GAATGGCAGG ACTCTGGTGG TTCAGGATGA GCCTCTAGGT 
GGGGACATGC CTGTACCTTT CACTCCATAT TCTTCAGCCA CGGGGCAGTC ACCTTTGGTT 
ACAGAGGTGG GTGGAGCTGG AAACAAGGAG GCTGGAAAAT CACTGGATTC AGGTGCTCAG 
AGTCTGGCAG TTTCAACAGC CGTGATCACA ACCACCAGCT CAACACCAAC CACCGTGAGA 
ATCTCTGATA CAGGCCTTTC AGCAGTGACC CTGCAGGAGA AACAAAAAGT CAACTGGTCT 
CAGACTCAGG GAGAGAATTC CAGAAACCCT CTCTTGGCTT CTTCTGGATT TGGAGCATCT 
CTCTCAAGTT CTTCCCAATC CTTGGCTTTT GGAAGTGTTA GAGCCCAGTC CAATGGGGTC 
TTGGCTACAG AGAGCAAGCC TTTGGGGTTT TCTTTTGCTT CCAGTTCTGC TACAGAAACC 
CAGAAAGATT CTGATCTTTC AAAGAACTTG TTTTTTCAGT GCATGTCACA GACTTTGCCC 
TCAAGTAACT ACTTCACAGC TATTTCAGAG AGTTTATCTG ATGAATCCTC TGCTCGGGAG 
TCATTCAAAC AGAGCCTAGA GAGCCTGAGC TCTGGGCTTT GTAAAGGCAA ACCTACCCTG 
GCAGCAGATA CGAAGGCTGG CAGTGCCCTG GATCGAAAGA TGCCTGCAGA GTCCATGCCT 
ACCCTCACTC CAGCCTTCCC AAGGAGCCTC TTGAATACCC GAGCCCCAGA GAATCATGAA 
AATCTATTTT TGCAGCCCCC TAAATTGTCC CGTGAAGAGC CATCCAACCC TTTCCTGGCA 
TTTGCAGAGA AAGCTGATCT CAGTCCTTTC AGCAGTTTTA CATCTCAGAC AGCAGGTGGC 
TCCACTTCTG CTACCACTGG AGCACTCAAA GCAGCTTCTA GCTGGCCAGA GTCTCACACC 
TCTGCTGATT CAGCATCTTT AGCAAAGAAG AAATCTCTCT TCATCACAAC TGATTCTTCC 
AAGCTAGGCT CTAGTGCACC TAGCTCAGCT GTGCCTCCTG GTGTCCCAAG CCTCTCTGCC 
ATGGGAAATG GCCGCTCTAA TTCACCCACC AGCAGTCTTT CACAACCTAT TGAGATGCCC 
ACCCTCTCCT CCAGCCCAAC AGAGGAAAAG CCATCAGTTG GACCTGGGCA ACAGGACAAT 
CCCCTTTTGA AAACCTTTAC TAATGTCTTT GGCAGGCACC CAACTGGCTT TCTCTCCTCA 
ACAGAGTTTT TACATGATAA CAAAGCTCCT TTTGAAGCTG TAAAAAGGTT TTCACTGGAT 
GAGCGAAGTA TGACATCTAA ACAGGACTCA GATTCCAGCA CCAATAGTGA CCTGTCAGAC 
CTGAGTGACT CTGAGGAGCA GCTGCAAGCC AAGGCTGGGT TGAAGGGAAT CCCCGAGCAC 
CTAATGGGAA AGCTGGGCCC CAATGGAGAG CGAAATGCTG AACTATTACT GGGAAAAGGA 
AGAGGGAAGC AGGCTCCTAA AGGCCGACCT CGAACAGCCC CTCTGAAAGT TGGCCAATCT 
GTGCTAAAAG ACATGAGCAA GGTAAGAAAG CTCAAACAGT CAGGAGAGCC TTTCCTCCAA 
GATGGATCCT GCATTAATGT GGCCCCTCAC CTCCATAAGT GCAGGGAGTG CCGCCTGGAG 
CGCTACCGAA AGTTTAAAGA GCAAGAACAA GATGATTCCA CTGTGGCCTG CCGATTCTTT 
CACTTCCGCA GGCTTATCTT CACTCGGAAA GGCATCCTCC GTGTAGAAGG GTTTTTGAGT 
CCGCAGCAAA GTGATCCAGA AGCCATGAAT CTGTGGATTC CCTCTTCCTC CCTTGCAGAA 
GGAATCGATC TGGAGACCTC AAAATACATA CTAGCCAATG TCGGGGACCA GTTTTGCCAG 
CTTGTCATGT CAGAAAAGGA GGCCATGATG ATGGTAGAGC CACACCAAAA AGTGGCATGG 
AAACGAGCTG TACGTGGTGT CAGGGAAATG TGTGATGTAT GTGAAACAAC TCTCTTCAAC 
ATCCATTGGG TTTGTCGCAA ATGTGGATTT GGAGTCTGTC TGGACTGTTA CCGACTTAGA 
AAGAGTCGTC CACGTAGTGA GACCGAAGAG ATTGGTGATG AAGAGGTTTT CTCTTGGTTG 
AAATGTGCAA AGGGGCAGTC ACATGAACCA GAAAATCTCA TGCCTACACA AATCATCCCT 
GGCACAGCTC TTTACAATAT TGGAGATATG GTACATGCAG CCCGGGGCAA GTGGGGAATT 
AAAGCCAATT GTCCTTGCAT TAGTCGTCAG AATAAATCTG TATTGAGGCC TGCTGTCACT 
AATGGGATTT CACAGCTTCC CAGTATAAAT CCCAGTGCTT CCTCTTCTGG AAGTGAAACT 
CCCTTCTCCA GTGGAGGAGG GACAACAGGG GTGACAACAC CAGAGTCTGA CCTATTACTC 
AAACCTGACA CTCTGGACAG CAGGGGTGAG GAGGCACTGA AAACTGATGC ATCAAGTAGC 
AGTAGTGAGA CAAAAACCAG CAGGCCACTG TGCCCCGAAA CAACACCACC ATCCTCTGCT 
TTGCACTGGC TGGCAGATTT AGCAACACAG AAAGCAAAGG AAGAAACAAA AGAAGCTGGA 
TCCCTGAGAT CAGTACTCAG TAAGGAGTCT CATTCCCCCT TTGGGCTGGA TTCCTTCAAC 
TCTACAGCAA AGGTTTCTCC ATTAACTCCA AAGCTCTTTA ATAGTCTGCT ACTGGGTCCT 
GCTGCCTCAA ACAACAAAAC AGAAGGCTCC AGCCTTCGAG ACCTACTACA CTCTGGACCC 
GGAAAGCTTC CCCAGGCCCC CTTGGACACC AGCATACCTT TCCCTCCAGT CTTCTCAGCA 
TCTTCAACAG GCGTGAAGAA TAAGGCCAGT CTTCCCAACT TCCTCGACCA CATCATTGCC 
TCAGTGGTAG AAAATAAAAA AACTTCTGAT GCTGCAAAAC GGGCCAGTAA CTTGACAGAT 
ACCCAAAAAG AAGTGAAGGA GATGGTAATG GGGCTGAATG TGTTGGATCC ACACACCTCC 
CACTCATGGC TCTGTGATGG GAGGCTCCTA TGTCTCCATG ACCCCAGCAA CAAAAACAAC 
TGGAAGATCT TTCGGGAGTG TTGGAAGCAA GGCCAGATCC ATAAAGAAGA GAAAGGGTGC 
AGGATTTCTG GAGTTATCCT TTTGAAATAT GAAATGTCAC CAGAAGTTGA TTTTTTTCAG 
TGTTTGGCCT TCCACTTTCT TTACATAGTA CCTGTCCTTG TTTCCGGGGT GCATAAGAAG 
CTCAAATCTG AACTTTGGAA GCCAGAAGCA TTTAGTCAAG AATTTGGAGA CCAGGATGTG 
GACTTAGTAA ACTGTAGGAA CTGTGCTATC ATTTCTGATG TGAAAGTGCG TGACTTCTGG 
GATGGTTTCG AAATCATATG CAAACGGCTA AGGTCAGAAG ATGGGCAGCC AATGGTACTG 
AAACTCAAAG ACTGGCCTCC AGGGGAAGAT TTCCGAGACA TGATGCCTAC AAGGTTTGAG 
GATCTGATGG AGAACCTCCC TCTGCCAGAG TACACGAAGC GAGATGGAAG GCTGAATTTG 
GCTTCTAGAC TACCCAGCTA TTTTGTACGA CCCGATTTGG GTCCCAAGAT GTACAATGCC 
TATGGTTTAA TCACTGCAGA AGATAGAAGA GTTGGTACAA CAAACTTGCA CTTAGATGTA 
TCTGATGCAG TTAATGTGAT GGTGTATGTT GGAATCCCCA TTGGGGAGGG AGCTCATGAT 
GAAGAGGTGC TAAAGACTAT TGATGAGGGT GATGCTGATG AGGTAACAAA ACAGCGGATT 
CATGAAGGGA ATGAGAAACC TGGAGCGTTA TGGCATATCT ATGCAGCTAA GGATGCTGAG 
AAGATCCGAG AACTTCTCCG AAAGGTTGGA GAAGAGCAAG GTCAAGAAAA TCCTCCAGAT 
CATGATCCAA TCCATGACCA AAGCTGGTAT CTGGACCAGA CTCTTCGTAA ACGTCTCTAT 
GAGGAGTATG GCGTGCAAGG CTGGGCAATT GTGCAGTTTC TGGGAGATGC TGTCTTTATA 
CCTGCAGGAG CCCCTCACCA AGTTCATAAT CTGTACAGTT GCATAAAAGT GGCAGAAGAT 
TTTGTATCTC CTGAACATGT AAAGCACTGT TTCCGCTTGA CCCAGGAGTT CAGACATCTG 
TCTAACACCC ACACAAATCA TGAGGATAAA CTGCAGGTAA AGAATATCAT TTACCATGCA 
GTGAAGGATG CTGTTGGCAC ACTGAAGGCT CATGAATCCA AACTGGCAAG ATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031809202.1
CDS	812..6628
Translation	MKNHDSCLEYLRRGRCLSEARVGGRRKKDTPPRPACNSPPDRRRRCSEGRLGVGRCERPGAAAPLRTAGGAVRSPGLRLRSRPRGALHSGGGWWWLGGGGEGGRGCGGLAGGSGAQRRQLLGLGRRWERRVRPALVMADAAASPVGKRLLLLFADAAAASAQAPVAAAAAAAAAAAAAAAESGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPFLLQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEFLLDRDLRFLSDATGLRLFQMGTESQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVKGQRVKVYQPEGEEGWICGVVSRQDSLTRLMEVSLSESGEIKSVDPRLIHVMLMDNSASQNEGSTLKAVKPPKGKKKRESIEGKDGRRRKSASDSGCDPATKKLKGERSEVDSNGSDGSETSRGPWKGSSSGEPGLDQRAKQLPPFVPQINRNIRFATYTKENGRTLVVQDEPLGGDMPVPFTPYSSATGQSPLVTEVGGAGNKEAGKSLDSGAQSLAVSTAVITTTSSTPTTVRISDTGLSAVTLQEKQKVNWSQTQGENSRNPLLASSGFGASLSSSSQSLAFGSVRAQSNGVLATESKPLGFSFASSSATETQKDSDLSKNLFFQCMSQTLPSSNYFTAISESLSDESSARESFKQSLESLSSGLCKGKPTLAADTKAGSALDRKMPAESMPTLTPAFPRSLLNTRAPENHENLFLQPPKLSREEPSNPFLAFAEKADLSPFSSFTSQTAGGSTSATTGALKAASSWPESHTSADSASLAKKKSLFITTDSSKLGSSAPSSAVPPGVPSLSAMGNGRSNSPTSSLSQPIEMPTLSSSPTEEKPSVGPGQQDNPLLKTFTNVFGRHPTGFLSSTEFLHDNKAPFEAVKRFSLDERSMTSKQDSDSSTNSDLSDLSDSEEQLQAKAGLKGIPEHLMGKLGPNGERNAELLLGKGRGKQAPKGRPRTAPLKVGQSVLKDMSKVRKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGILRVEGFLSPQQSDPEAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEIGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGISQLPSINPSASSSGSETPFSSGGGTTGVTTPESDLLLKPDTLDSRGEEALKTDASSSSSETKTSRPLCPETTPPSSALHWLADLATQKAKEETKEAGSLRSVLSKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPAASNNKTEGSSLRDLLHSGPGKLPQAPLDTSIPFPPVFSASSTGVKNKASLPNFLDHIIASVVENKKTSDAAKRASNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQIHKEEKGCRISGVILLKYEMSPEVDFFQCLAFHFLYIVPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHEGNEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_031953342.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031953342.1

ORF Insert Sequence:

ATGAAGAACC ATGACTCCTG CCTCGAGTAC CTTCGTAGAG GGCGCTGTTT ATCAGAGGCC 
AGAGTTGGGG GCAGAAGAAA AAAGGACACC CCACCCCGCC CAGCGTGCAA TTCCCCTCCC 
GACCGCAGGA GGCGCTGCAG CGAGGGGAGA CTTGGGGTGG GGCGCTGTGA GAGGCCCGGG 
GCTGCGGCCC CGCTCCGGAC CGCGGGGGGC GCTGTGAGGA GCCCGGGGCT GCGGCTCCGC 
TCCCGGCCGC GGGGGGCGCT GCACAGCGGC GGTGGTTGGT GGTGGCTTGG GGGTGGGGGG 
GAGGGGGGTA GGGGGTGCGG AGGCCTCGCG GGCGGATCGG GCGCTCAGCG GCGGCAGCTT 
CTGGGCTTGG GGAGGCGGTG GGAGCGGCGG GTCCGGCCGG CCCTGGTCAT GGCGGACGCG 
GCGGCCTCTC CGGTGGGGAA GCGGCTGCTG CTTCTGTTTG CGGATGCGGC GGCGGCCTCG 
GCTCAGGCAC CGGTGGCGGC GGCGGCGGCG GCAGCGGCAG CGGCAGCGGC AGCGGCAGCA 
GAGAGCGGGG ATCCGGGGCC TGCGCTGCGC ACTCGGGCCT GGAGGGCTGG GACAGTGCGG 
GCCATGAGCG GGGCGGTGCC CCAGGATCTA GCGATATTTG TGGAATTTGA TGGCTGCAAC 
TGGAAGCAAC ACTCCTGGGT GAAAGTCCAT GCTGAAGAGG TTATTGTGCT GCTCCTAGAA 
GGCTCTTTAG TTTGGGCTCC TCGAAAGGAT CCATTCCTCC TCCAGGGGAC CAGGGTCTCA 
ATTGCACAGT GGCCAGCCCT GACCTTCACT CCATTAGTGG ATAAATTGGG TTTGGGGTCT 
GTGGTTCCAG TGGAATTTCT TCTGGATCGA GATCTCCGAT TCTTGTCAGA TGCTACTGGA 
TTACGCCTAT TCCAGATGGG AACAGAGAGC CAGAACCAGA TTCTTCTAGA ACATGCTGCA 
TTGAGAGATA CAGTTAATGC TTTGATCAGT GACCAAAAGC TCCAGGAAAT ATTCAGTCGA 
GGTCCCTATA GTGTCAAAGG CCAGAGGGTC AAAGTGTACC AGCCAGAAGG TGAAGAAGGT 
TGGATCTGTG GTGTTGTAAG CCGACAAGAC TCTCTCACTC GTCTTATGGA GGTATCTTTG 
TCAGAGAGTG GTGAGATTAA ATCAGTGGAT CCCAGATTGA TACATGTGAT GTTGATGGAC 
AACTCTGCTT CTCAGAATGA GGGAAGCACA TTAAAAGCTG TAAAACCCCC CAAAGGAAAG 
AAGAAAAGAG AGAGCATAGA GGGAAAAGAT GGACGAAGGA GGAAAAGTGC TTCAGACTCT 
GGGTGTGACC CTGCAACAAA GAAATTAAAA GGAGAAAGGA GTGAAGTAGA CAGTAATGGG 
AGTGATGGAA GTGAAACAAG TCGAGGGCCC TGGAAAGGGA GTTCTAGTGG AGAGCCAGGG 
CTGGATCAAA GGGCCAAGCA GCTGCCTCCA TTTGTTCCCC AGATCAATCG AAACATTCGA 
TTTGCCACAT ACACCAAAGA GAATGGCAGG ACTCTGGTGG TTCAGGATGA GCCTCTAGGT 
GGGGACATGC CTGTACCTTT CACTCCATAT TCTTCAGCCA CGGGGCAGTC ACCTTTGGTT 
ACAGAGGTGG GTGGAGCTGG AAACAAGGAG GCTGGAAAAT CACTGGATTC AGGTGCTCAG 
AGTCTGGCAG TTTCAACAGC CGTGATCACA ACCACCAGCT CAACACCAAC CACCGTGAGA 
ATCTCTGATA CAGGCCTTTC AGCAGTGACC CTGCAGGAGA AACAAAAAGT CAACTGGTCT 
CAGACTCAGG GAGAGAATTC CAGAAACCCT CTCTTGGCTT CTTCTGGATT TGGAGCATCT 
CTCTCAAGTT CTTCCCAATC CTTGGCTTTT GGAAGTGTTA GAGCCCAGTC CAATGGGGTC 
TTGGCTACAG AGAGCAAGCC TTTGGGGTTT TCTTTTGCTT CCAGTTCTGC TACAGAAACC 
CAGAAAGATT CTGATCTTTC AAAGAACTTG TTTTTTCAGT GCATGTCACA GACTTTGCCC 
TCAAGTAACT ACTTCACAGC TATTTCAGAG AGTTTATCTG ATGAATCCTC TGCTCGGGAG 
TCATTCAAAC AGAGCCTAGA GAGCCTGAGC TCTGGGCTTT GTAAAGGCAA ACCTACCCTG 
GCAGCAGATA CGAAGGCTGG CAGTGCCCTG GATCGAAAGA TGCCTGCAGA GTCCATGCCT 
ACCCTCACTC CAGCCTTCCC AAGGAGCCTC TTGAATACCC GAGCCCCAGA GAATCATGAA 
AATCTATTTT TGCAGCCCCC TAAATTGTCC CGTGAAGAGC CATCCAACCC TTTCCTGGCA 
TTTGCAGAGA AAGCTGATCT CAGTCCTTTC AGCAGTTTTA CATCTCAGAC AGCAGGTGGC 
TCCACTTCTG CTACCACTGG AGCACTCAAA GCAGCTTCTA GCTGGCCAGA GTCTCACACC 
TCTGCTGATT CAGCATCTTT AGCAAAGAAG AAATCTCTCT TCATCACAAC TGATTCTTCC 
AAGCTAGGCT CTAGTGCACC TAGCTCAGCT GTGCCTCCTG GTGTCCCAAG CCTCTCTGCC 
ATGGGAAATG GCCGCTCTAA TTCACCCACC AGCAGTCTTT CACAACCTAT TGAGATGCCC 
ACCCTCTCCT CCAGCCCAAC AGAGGAAAAG CCATCAGTTG GACCTGGGCA ACAGGACAAT 
CCCCTTTTGA AAACCTTTAC TAATGTCTTT GGCAGGCACC CAACTGGCTT TCTCTCCTCA 
ACAGAGTTTT TACATGATAA CAAAGCTCCT TTTGAAGCTG TAAAAAGGTT TTCACTGGAT 
GAGCGAAGTA TGACATCTAA ACAGGACTCA GATTCCAGCA CCAATAGTGA CCTGTCAGAC 
CTGAGTGACT CTGAGGAGCA GCTGCAAGCC AAGGCTGGGT TGAAGGGAAT CCCCGAGCAC 
CTAATGGGAA AGCTGGGCCC CAATGGAGAG CGAAATGCTG AACTATTACT GGGAAAAGGA 
AGAGGGAAGC AGGCTCCTAA AGGCCGACCT CGAACAGCCC CTCTGAAAGT TGGCCAATCT 
GTGCTAAAAG ACATGAGCAA GGTAAGAAAG CTCAAACAGT CAGGAGAGCC TTTCCTCCAA 
GATGGATCCT GCATTAATGT GGCCCCTCAC CTCCATAAGT GCAGGGAGTG CCGCCTGGAG 
CGCTACCGAA AGTTTAAAGA GCAAGAACAA GATGATTCCA CTGTGGCCTG CCGATTCTTT 
CACTTCCGCA GGCTTATCTT CACTCGGAAA GGCATCCTCC GTGTAGAAGG GTTTTTGAGT 
CCGCAGCAAA GTGATCCAGA AGCCATGAAT CTGTGGATTC CCTCTTCCTC CCTTGCAGAA 
GGAATCGATC TGGAGACCTC AAAATACATA CTAGCCAATG TCGGGGACCA GTTTTGCCAG 
CTTGTCATGT CAGAAAAGGA GGCCATGATG ATGGTAGAGC CACACCAAAA AGTGGCATGG 
AAACGAGCTG TACGTGGTGT CAGGGAAATG TGTGATGTAT GTGAAACAAC TCTCTTCAAC 
ATCCATTGGG TTTGTCGCAA ATGTGGATTT GGAGTCTGTC TGGACTGTTA CCGACTTAGA 
AAGAGTCGTC CACGTAGTGA GACCGAAGAG ATTGGTGATG AAGAGGTTTT CTCTTGGTTG 
AAATGTGCAA AGGGGCAGTC ACATGAACCA GAAAATCTCA TGCCTACACA AATCATCCCT 
GGCACAGCTC TTTACAATAT TGGAGATATG GTACATGCAG CCCGGGGCAA GTGGGGAATT 
AAAGCCAATT GTCCTTGCAT TAGTCGTCAG AATAAATCTG TATTGAGGCC TGCTGTCACT 
AATGGGATTT CACAGCTTCC CAGTATAAAT CCCAGTGCTT CCTCTTCTGG AAGTGAAACT 
CCCTTCTCCA GTGGAGGAGG GACAACAGGG GTGACAACAC CAGAGTCTGA CCTATTACTC 
AAACCTGACA CTCTGGACAG CAGGGGTGAG GAGGCACTGA AAACTGATGC ATCAAGTAGC 
AGTAGTGAGA CAAAAACCAG CAGGCCACTG TGCCCCGAAA CAACACCACC ATCCTCTGCT 
TTGCACTGGC TGGCAGATTT AGCAACACAG AAAGCAAAGG AAGAAACAAA AGAAGCTGGA 
TCCCTGAGAT CAGTACTCAG TAAGGAGTCT CATTCCCCCT TTGGGCTGGA TTCCTTCAAC 
TCTACAGCAA AGGTTTCTCC ATTAACTCCA AAGCTCTTTA ATAGTCTGCT ACTGGGTCCT 
GCTGCCTCAA ACAACAAAAC AGAAGGCTCC AGCCTTCGAG ACCTACTACA CTCTGGACCC 
GGAAAGCTTC CCCAGGCCCC CTTGGACACC AGCATACCTT TCCCTCCAGT CTTCTCAGCA 
TCTTCAACAG GCGTGAAGAA TAAGGCCAGT CTTCCCAACT TCCTCGACCA CATCATTGCC 
TCAGTGGTAG AAAATAAAAA AACTTCTGAT GCTGCAAAAC GGGCCAGTAA CTTGACAGAT 
ACCCAAAAAG AAGTGAAGGA GATGGTAATG GGGCTGAATG TGTTGGATCC ACACACCTCC 
CACTCATGGC TCTGTGATGG GAGGCTCCTA TGTCTCCATG ACCCCAGCAA CAAAAACAAC 
TGGAAGATCT TTCGGGAGTG TTGGAAGCAA GGCCAGATCC ATAAAGAAGA GAAAGGGTGC 
AGGATTTCTG GAGTTATCCT TTTGAAATAT GAAATGTCAC CAGAAGTTGA TTTTTTTCAG 
TGTTTGGCCT TCCACTTTCT TTACATAGTA CCTGTCCTTG TTTCCGGGGT GCATAAGAAG 
CTCAAATCTG AACTTTGGAA GCCAGAAGCA TTTAGTCAAG AATTTGGAGA CCAGGATGTG 
GACTTAGTAA ACTGTAGGAA CTGTGCTATC ATTTCTGATG TGAAAGTGCG TGACTTCTGG 
GATGGTTTCG AAATCATATG CAAACGGCTA AGGTCAGAAG ATGGGCAGCC AATGGTACTG 
AAACTCAAAG ACTGGCCTCC AGGGGAAGAT TTCCGAGACA TGATGCCTAC AAGGTTTGAG 
GATCTGATGG AGAACCTCCC TCTGCCAGAG TACACGAAGC GAGATGGAAG GCTGAATTTG 
GCTTCTAGAC TACCCAGCTA TTTTGTACGA CCCGATTTGG GTCCCAAGAT GTACAATGCC 
TATGGTTTAA TCACTGCAGA AGATAGAAGA GTTGGTACAA CAAACTTGCA CTTAGATGTA 
TCTGATGCAG TTAATGTGAT GGTGTATGTT GGAATCCCCA TTGGGGAGGG AGCTCATGAT 
GAAGAGGTGC TAAAGACTAT TGATGAGGGT GATGCTGATG AGGTAACAAA ACAGCGGATT 
CATGAAGGGA ATGAGAAACC TGGAGCGTTA TGGCATATCT ATGCAGCTAA GGATGCTGAG 
AAGATCCGAG AACTTCTCCG AAAGGTTGGA GAAGAGCAAG GTCAAGAAAA TCCTCCAGAT 
CATGATCCAA TCCATGACCA AAGCTGGTAT CTGGACCAGA CTCTTCGTAA ACGTCTCTAT 
GAGGAGTATG GCGTGCAAGG CTGGGCAATT GTGCAGTTTC TGGGAGATGC TGTCTTTATA 
CCTGCAGGAG CCCCTCACCA AGTTCATAAT CTGTACAGTT GCATAAAAGT GGCAGAAGAT 
TTTGTATCTC CTGAACATGT AAAGCACTGT TTCCGCTTGA CCCAGGAGTT CAGACATCTG 
TCTAACACCC ACACAAATCA TGAGGATAAA CTGCAGGTAA AGAATATCAT TTACCATGCA 
GTGAAGGATG CTGTTGGCAC ACTGAAGGCT CATGAATCCA AACTGGCAAG ATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_031953343.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031809203.1 Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X2, mRNA.

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CloneID	OSa225683
Clone ID Related Accession (Same CDS sequence)	XM_031953343.1
Accession Version	XM_031953343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	lysine-specific demethylase 3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACC ATGACTCCTG CCTCGAGTAC CTTCGTAGAG GGCGCTGTTT ATCAGAGGCC 
AGAGTTGGGG GCAGAAGAAA AAAGGACACC CCACCCCGCC CAGCGTGCAA TTCCCCTCCC 
GACCGCAGGA GGCGCTGCAG CGAGGGGAGA CTTGGGGTGG GGCGCTGTGA GAGGCCCGGG 
GCTGCGGCCC CGCTCCGGAC CGCGGGGGGC GCTGTGAGGA GCCCGGGGCT GCGGCTCCGC 
TCCCGGCCGC GGGGGGCGCT GCACAGCGGC GGTGGTTGGT GGTGGCTTGG GGGTGGGGGG 
GAGGGGGGTA GGGGGTGCGG AGGCCTCGCG GGCGGATCGG GCGCTCAGCG GCGGCAGCTT 
CTGGGCTTGG GGAGGCGGTG GGAGCGGCGG GTCCGGCCGG CCCTGGTCAT GGCGGACGCG 
GCGGCCTCTC CGGTGGGGAA GCGGCTGCTG CTTCTGTTTG CGGATGCGGC GGCGGCCTCG 
GCTCAGGCAC CGGTGGCGGC GGCGGCGGCG GCAGCGGCAG CGGCAGCGGC AGCGGCAGCA 
GAGAGCGGGG ATCCGGGGCC TGCGCTGCGC ACTCGGGCCT GGAGGGCTGG GACAGTGCGG 
GCCATGAGCG GGGCGGTGCC CCAGGATCTA GCGATATTTG TGGAATTTGA TGGCTGCAAC 
TGGAAGCAAC ACTCCTGGGT GAAAGTCCAT GCTGAAGAGG TTATTGTGCT GCTCCTAGAA 
GGCTCTTTAG TTTGGGCTCC TCGAAAGGAT CCATTCCTCC TCCAGGGGAC CAGGGTCTCA 
ATTGCACAGT GGCCAGCCCT GACCTTCACT CCATTAGTGG ATAAATTGGG TTTGGGGTCT 
GTGGTTCCAG TGGAATTTCT TCTGGATCGA GATCTCCGAT TCTTGTCAGA TGCTACTGGA 
TTACGCCTAT TCCAGATGGG AACAGAGAGC CAGAACCAGA TTCTTCTAGA ACATGCTGCA 
TTGAGAGATA CAGTTAATGC TTTGATCAGT GACCAAAAGC TCCAGGAAAT ATTCAGTCGA 
GGTCCCTATA GTGTCAAAGG CCAGAGGGTC AAAGTGTACC AGCCAGAAGG TGAAGAAGGT 
TGGATCTGTG GTGTTGTAAG CCGACAAGAC TCTCTCACTC GTCTTATGGA GGTATCTTTG 
TCAGAGAGTG GTGAGATTAA ATCAGTGGAT CCCAGATTGA TACATGTGAT GTTGATGGAC 
AACTCTGCTT CTCAGAATGA GGGAAGCACA TTAAAAGCTG TAAAACCCCC CAAAGGAAAG 
AAGAAAAGAG AGAGCATAGA GGGAAAAGAT GGACGAAGGA GGAAAAGTGC TTCAGACTCT 
GGGTGTGACC CTGCAACAAA GAAATTAAAA GGAGAAAGGA GTGAAGTAGA CAGTAATGGG 
AGTGATGGAA GTGAAACAAG TCGAGGGCCC TGGAAAGGGA GTTCTAGTGG AGAGCCAGGG 
CTGGATCAAA GGGCCAAGCA GCTGCCTCCA TTTGTTCCCC AGATCAATCG AAACATTCGA 
TTTGCCACAT ACACCAAAGA GAATGGCAGG ACTCTGGTGG TTCAGGATGA GCCTCTAGGT 
GGGGACATGC CTGTACCTTT CACTCCATAT TCTTCAGCCA CGGGGCAGTC ACCTTTGGTT 
ACAGAGGTGG GTGGAGCTGG AAACAAGGAG GCTGGAAAAT CACTGGATTC AGGTGCTCAG 
AGTCTGGCAG TTTCAACAGC CGTGATCACA ACCACCAGCT CAACACCAAC CACCGTGAGA 
ATCTCTGATA CAGGCCTTTC AGCAGTGACC CTGCAGGAGA AACAAAAAGT CAACTGGTCT 
CAGACTCAGG GAGAGAATTC CAGAAACCCT CTCTTGGCTT CTTCTGGATT TGGAGCATCT 
CTCTCAAGTT CTTCCCAATC CTTGGCTTTT GGAAGTGTTA GAGCCCAGTC CAATGGGGTC 
TTGGCTACAG AGAGCAAGCC TTTGGGGTTT TCTTTTGCTT CCAGTTCTGC TACAGAAACC 
CAGAAAGATT CTGATCTTTC AAAGAACTTG TTTTTTCAGT GCATGTCACA GACTTTGCCC 
TCAAGTAACT ACTTCACAGC TATTTCAGAG AGTTTATCTG ATGAATCCTC TGCTCGGGAG 
TCATTCAAAC AGAGCCTAGA GAGCCTGAGC TCTGGGCTTT GTAAAGGCAA ACCTACCCTG 
GCAGCAGATA CGAAGGCTGG CAGTGCCCTG GATCGAAAGA TGCCTGCAGA GTCCATGCCT 
ACCCTCACTC CAGCCTTCCC AAGGAGCCTC TTGAATACCC GAGCCCCAGA GAATCATGAA 
AATCTATTTT TGCAGCCCCC TAAATTGTCC CGTGAAGAGC CATCCAACCC TTTCCTGGCA 
TTTGCAGAGA AAGCTGATCT CAGTCCTTTC AGCAGTTTTA CATCTCAGAC AGCAGGTGGC 
TCCACTTCTG CTACCACTGG AGCACTCAAA GCAGCTTCTA GCTGGCCAGA GTCTCACACC 
TCTGCTGATT CAGCATCTTT AGCAAAGAAG AAATCTCTCT TCATCACAAC TGATTCTTCC 
AAGCTAGGCT CTAGTGCACC TAGCTCAGCT GTGCCTCCTG GTGTCCCAAG CCTCTCTGCC 
ATGGGAAATG GCCGCTCTAA TTCACCCACC AGCAGTCTTT CACAACCTAT TGAGATGCCC 
ACCCTCTCCT CCAGCCCAAC AGAGGAAAAG CCATCAGTTG GACCTGGGCA ACAGGACAAT 
CCCCTTTTGA AAACCTTTAC TAATGTCTTT GGCAGGCACC CAACTGGCTT TCTCTCCTCA 
ACAGAGTTTT TACATGATAA CAAAGCTCCT TTTGAAGCTG TAAAAAGGTT TTCACTGGAT 
GAGCGAAGTA TGACATCTAA ACAGGACTCA GATTCCAGCA CCAATAGTGA CCTGTCAGAC 
CTGAGTGACT CTGAGGAGCA GCTGCAAGCC AAGGCTGGGT TGAAGGGAAT CCCCGAGCAC 
CTAATGGGAA AGCTGGGCCC CAATGGAGAG CGAAATGCTG AACTATTACT GGGAAAAGGA 
AGAGGGAAGC AGGCTCCTAA AGGCCGACCT CGAACAGCCC CTCTGAAAGT TGGCCAATCT 
GTGCTAAAAG ACATGAGCAA GGTAAGAAAG CTCAAACAGT CAGGAGAGCC TTTCCTCCAA 
GATGGATCCT GCATTAATGT GGCCCCTCAC CTCCATAAGT GCAGGGAGTG CCGCCTGGAG 
CGCTACCGAA AGTTTAAAGA GCAAGAACAA GATGATTCCA CTGTGGCCTG CCGATTCTTT 
CACTTCCGCA GGCTTATCTT CACTCGGAAA GGCATCCTCC GTGTAGAAGG GTTTTTGAGT 
CCGCAGCAAA GTGATCCAGA AGCCATGAAT CTGTGGATTC CCTCTTCCTC CCTTGCAGAA 
GGAATCGATC TGGAGACCTC AAAATACATA CTAGCCAATG TCGGGGACCA GTTTTGCCAG 
CTTGTCATGT CAGAAAAGGA GGCCATGATG ATGGTAGAGC CACACCAAAA AGTGGCATGG 
AAACGAGCTG TACGTGGTGT CAGGGAAATG TGTGATGTAT GTGAAACAAC TCTCTTCAAC 
ATCCATTGGG TTTGTCGCAA ATGTGGATTT GGAGTCTGTC TGGACTGTTA CCGACTTAGA 
AAGAGTCGTC CACGTAGTGA GACCGAAGAG ATTGGTGATG AAGAGGTTTT CTCTTGGTTG 
AAATGTGCAA AGGGGCAGTC ACATGAACCA GAAAATCTCA TGCCTACACA AATCATCCCT 
GGCACAGCTC TTTACAATAT TGGAGATATG GTACATGCAG CCCGGGGCAA GTGGGGAATT 
AAAGCCAATT GTCCTTGCAT TAGTCGTCAG AATAAATCTG TATTGAGGCC TGCTGTCACT 
AATGGGATTT CACAGCTTCC CAGTATAAAT CCCAGTGCTT CCTCTTCTGG AAGTGAAACT 
CCCTTCTCCA GTGGAGGAGG GACAACAGGG GTGACAACAC CAGAGTCTGA CCTATTACTC 
AAACCTGACA CTCTGGACAG CAGGGGTGAG GAGGCACTGA AAACTGATGC ATCAAGTAGC 
AGTAGTGAGA CAAAAACCAG CAGGCCACTG TGCCCCGAAA CAACACCACC ATCCTCTGCT 
TTGCACTGGC TGGCAGATTT AGCAACACAG AAAGCAAAGG AAGAAACAAA AGAAGCTGGA 
TCCCTGAGAT CAGTACTCAG TAAGGAGTCT CATTCCCCCT TTGGGCTGGA TTCCTTCAAC 
TCTACAGCAA AGGTTTCTCC ATTAACTCCA AAGCTCTTTA ATAGTCTGCT ACTGGGTCCT 
GCTGCCTCAA ACAACAAAAC AGAAGGCTCC AGCCTTCGAG ACCTACTACA CTCTGGACCC 
GGAAAGCTTC CCCAGGCCCC CTTGGACACC AGCATACCTT TCCCTCCAGT CTTCTCAGCA 
TCTTCAACAG GCGTGAAGAA TAAGGCCAGT CTTCCCAACT TCCTCGACCA CATCATTGCC 
TCAGTGGTAG AAAATAAAAA AACTTCTGAT GCTGCAAAAC GGGCCAGTAA CTTGACAGAT 
ACCCAAAAAG AAGTGAAGGA GATGGTAATG GGGCTGAATG TGTTGGATCC ACACACCTCC 
CACTCATGGC TCTGTGATGG GAGGCTCCTA TGTCTCCATG ACCCCAGCAA CAAAAACAAC 
TGGAAGATCT TTCGGGAGTG TTGGAAGCAA GGCCAGCCTG TCCTTGTTTC CGGGGTGCAT 
AAGAAGCTCA AATCTGAACT TTGGAAGCCA GAAGCATTTA GTCAAGAATT TGGAGACCAG 
GATGTGGACT TAGTAAACTG TAGGAACTGT GCTATCATTT CTGATGTGAA AGTGCGTGAC 
TTCTGGGATG GTTTCGAAAT CATATGCAAA CGGCTAAGGT CAGAAGATGG GCAGCCAATG 
GTACTGAAAC TCAAAGACTG GCCTCCAGGG GAAGATTTCC GAGACATGAT GCCTACAAGG 
TTTGAGGATC TGATGGAGAA CCTCCCTCTG CCAGAGTACA CGAAGCGAGA TGGAAGGCTG 
AATTTGGCTT CTAGACTACC CAGCTATTTT GTACGACCCG ATTTGGGTCC CAAGATGTAC 
AATGCCTATG GTTTAATCAC TGCAGAAGAT AGAAGAGTTG GTACAACAAA CTTGCACTTA 
GATGTATCTG ATGCAGTTAA TGTGATGGTG TATGTTGGAA TCCCCATTGG GGAGGGAGCT 
CATGATGAAG AGGTGCTAAA GACTATTGAT GAGGGTGATG CTGATGAGGT AACAAAACAG 
CGGATTCATG AAGGGAATGA GAAACCTGGA GCGTTATGGC ATATCTATGC AGCTAAGGAT 
GCTGAGAAGA TCCGAGAACT TCTCCGAAAG GTTGGAGAAG AGCAAGGTCA AGAAAATCCT 
CCAGATCATG ATCCAATCCA TGACCAAAGC TGGTATCTGG ACCAGACTCT TCGTAAACGT 
CTCTATGAGG AGTATGGCGT GCAAGGCTGG GCAATTGTGC AGTTTCTGGG AGATGCTGTC 
TTTATACCTG CAGGAGCCCC TCACCAAGTT CATAATCTGT ACAGTTGCAT AAAAGTGGCA 
GAAGATTTTG TATCTCCTGA ACATGTAAAG CACTGTTTCC GCTTGACCCA GGAGTTCAGA 
CATCTGTCTA ACACCCACAC AAATCATGAG GATAAACTGC AGGTAAAGAA TATCATTTAC 
CATGCAGTGA AGGATGCTGT TGGCACACTG AAGGCTCATG AATCCAAACT GGCAAGATCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031809203.1
CDS	810..6512
Translation	MKNHDSCLEYLRRGRCLSEARVGGRRKKDTPPRPACNSPPDRRRRCSEGRLGVGRCERPGAAAPLRTAGGAVRSPGLRLRSRPRGALHSGGGWWWLGGGGEGGRGCGGLAGGSGAQRRQLLGLGRRWERRVRPALVMADAAASPVGKRLLLLFADAAAASAQAPVAAAAAAAAAAAAAAAESGDPGPALRTRAWRAGTVRAMSGAVPQDLAIFVEFDGCNWKQHSWVKVHAEEVIVLLLEGSLVWAPRKDPFLLQGTRVSIAQWPALTFTPLVDKLGLGSVVPVEFLLDRDLRFLSDATGLRLFQMGTESQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVKGQRVKVYQPEGEEGWICGVVSRQDSLTRLMEVSLSESGEIKSVDPRLIHVMLMDNSASQNEGSTLKAVKPPKGKKKRESIEGKDGRRRKSASDSGCDPATKKLKGERSEVDSNGSDGSETSRGPWKGSSSGEPGLDQRAKQLPPFVPQINRNIRFATYTKENGRTLVVQDEPLGGDMPVPFTPYSSATGQSPLVTEVGGAGNKEAGKSLDSGAQSLAVSTAVITTTSSTPTTVRISDTGLSAVTLQEKQKVNWSQTQGENSRNPLLASSGFGASLSSSSQSLAFGSVRAQSNGVLATESKPLGFSFASSSATETQKDSDLSKNLFFQCMSQTLPSSNYFTAISESLSDESSARESFKQSLESLSSGLCKGKPTLAADTKAGSALDRKMPAESMPTLTPAFPRSLLNTRAPENHENLFLQPPKLSREEPSNPFLAFAEKADLSPFSSFTSQTAGGSTSATTGALKAASSWPESHTSADSASLAKKKSLFITTDSSKLGSSAPSSAVPPGVPSLSAMGNGRSNSPTSSLSQPIEMPTLSSSPTEEKPSVGPGQQDNPLLKTFTNVFGRHPTGFLSSTEFLHDNKAPFEAVKRFSLDERSMTSKQDSDSSTNSDLSDLSDSEEQLQAKAGLKGIPEHLMGKLGPNGERNAELLLGKGRGKQAPKGRPRTAPLKVGQSVLKDMSKVRKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGILRVEGFLSPQQSDPEAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEIGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGISQLPSINPSASSSGSETPFSSGGGTTGVTTPESDLLLKPDTLDSRGEEALKTDASSSSSETKTSRPLCPETTPPSSALHWLADLATQKAKEETKEAGSLRSVLSKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPAASNNKTEGSSLRDLLHSGPGKLPQAPLDTSIPFPPVFSASSTGVKNKASLPNFLDHIIASVVENKKTSDAAKRASNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHEGNEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_031953343.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031953343.1

ORF Insert Sequence:

ATGAAGAACC ATGACTCCTG CCTCGAGTAC CTTCGTAGAG GGCGCTGTTT ATCAGAGGCC 
AGAGTTGGGG GCAGAAGAAA AAAGGACACC CCACCCCGCC CAGCGTGCAA TTCCCCTCCC 
GACCGCAGGA GGCGCTGCAG CGAGGGGAGA CTTGGGGTGG GGCGCTGTGA GAGGCCCGGG 
GCTGCGGCCC CGCTCCGGAC CGCGGGGGGC GCTGTGAGGA GCCCGGGGCT GCGGCTCCGC 
TCCCGGCCGC GGGGGGCGCT GCACAGCGGC GGTGGTTGGT GGTGGCTTGG GGGTGGGGGG 
GAGGGGGGTA GGGGGTGCGG AGGCCTCGCG GGCGGATCGG GCGCTCAGCG GCGGCAGCTT 
CTGGGCTTGG GGAGGCGGTG GGAGCGGCGG GTCCGGCCGG CCCTGGTCAT GGCGGACGCG 
GCGGCCTCTC CGGTGGGGAA GCGGCTGCTG CTTCTGTTTG CGGATGCGGC GGCGGCCTCG 
GCTCAGGCAC CGGTGGCGGC GGCGGCGGCG GCAGCGGCAG CGGCAGCGGC AGCGGCAGCA 
GAGAGCGGGG ATCCGGGGCC TGCGCTGCGC ACTCGGGCCT GGAGGGCTGG GACAGTGCGG 
GCCATGAGCG GGGCGGTGCC CCAGGATCTA GCGATATTTG TGGAATTTGA TGGCTGCAAC 
TGGAAGCAAC ACTCCTGGGT GAAAGTCCAT GCTGAAGAGG TTATTGTGCT GCTCCTAGAA 
GGCTCTTTAG TTTGGGCTCC TCGAAAGGAT CCATTCCTCC TCCAGGGGAC CAGGGTCTCA 
ATTGCACAGT GGCCAGCCCT GACCTTCACT CCATTAGTGG ATAAATTGGG TTTGGGGTCT 
GTGGTTCCAG TGGAATTTCT TCTGGATCGA GATCTCCGAT TCTTGTCAGA TGCTACTGGA 
TTACGCCTAT TCCAGATGGG AACAGAGAGC CAGAACCAGA TTCTTCTAGA ACATGCTGCA 
TTGAGAGATA CAGTTAATGC TTTGATCAGT GACCAAAAGC TCCAGGAAAT ATTCAGTCGA 
GGTCCCTATA GTGTCAAAGG CCAGAGGGTC AAAGTGTACC AGCCAGAAGG TGAAGAAGGT 
TGGATCTGTG GTGTTGTAAG CCGACAAGAC TCTCTCACTC GTCTTATGGA GGTATCTTTG 
TCAGAGAGTG GTGAGATTAA ATCAGTGGAT CCCAGATTGA TACATGTGAT GTTGATGGAC 
AACTCTGCTT CTCAGAATGA GGGAAGCACA TTAAAAGCTG TAAAACCCCC CAAAGGAAAG 
AAGAAAAGAG AGAGCATAGA GGGAAAAGAT GGACGAAGGA GGAAAAGTGC TTCAGACTCT 
GGGTGTGACC CTGCAACAAA GAAATTAAAA GGAGAAAGGA GTGAAGTAGA CAGTAATGGG 
AGTGATGGAA GTGAAACAAG TCGAGGGCCC TGGAAAGGGA GTTCTAGTGG AGAGCCAGGG 
CTGGATCAAA GGGCCAAGCA GCTGCCTCCA TTTGTTCCCC AGATCAATCG AAACATTCGA 
TTTGCCACAT ACACCAAAGA GAATGGCAGG ACTCTGGTGG TTCAGGATGA GCCTCTAGGT 
GGGGACATGC CTGTACCTTT CACTCCATAT TCTTCAGCCA CGGGGCAGTC ACCTTTGGTT 
ACAGAGGTGG GTGGAGCTGG AAACAAGGAG GCTGGAAAAT CACTGGATTC AGGTGCTCAG 
AGTCTGGCAG TTTCAACAGC CGTGATCACA ACCACCAGCT CAACACCAAC CACCGTGAGA 
ATCTCTGATA CAGGCCTTTC AGCAGTGACC CTGCAGGAGA AACAAAAAGT CAACTGGTCT 
CAGACTCAGG GAGAGAATTC CAGAAACCCT CTCTTGGCTT CTTCTGGATT TGGAGCATCT 
CTCTCAAGTT CTTCCCAATC CTTGGCTTTT GGAAGTGTTA GAGCCCAGTC CAATGGGGTC 
TTGGCTACAG AGAGCAAGCC TTTGGGGTTT TCTTTTGCTT CCAGTTCTGC TACAGAAACC 
CAGAAAGATT CTGATCTTTC AAAGAACTTG TTTTTTCAGT GCATGTCACA GACTTTGCCC 
TCAAGTAACT ACTTCACAGC TATTTCAGAG AGTTTATCTG ATGAATCCTC TGCTCGGGAG 
TCATTCAAAC AGAGCCTAGA GAGCCTGAGC TCTGGGCTTT GTAAAGGCAA ACCTACCCTG 
GCAGCAGATA CGAAGGCTGG CAGTGCCCTG GATCGAAAGA TGCCTGCAGA GTCCATGCCT 
ACCCTCACTC CAGCCTTCCC AAGGAGCCTC TTGAATACCC GAGCCCCAGA GAATCATGAA 
AATCTATTTT TGCAGCCCCC TAAATTGTCC CGTGAAGAGC CATCCAACCC TTTCCTGGCA 
TTTGCAGAGA AAGCTGATCT CAGTCCTTTC AGCAGTTTTA CATCTCAGAC AGCAGGTGGC 
TCCACTTCTG CTACCACTGG AGCACTCAAA GCAGCTTCTA GCTGGCCAGA GTCTCACACC 
TCTGCTGATT CAGCATCTTT AGCAAAGAAG AAATCTCTCT TCATCACAAC TGATTCTTCC 
AAGCTAGGCT CTAGTGCACC TAGCTCAGCT GTGCCTCCTG GTGTCCCAAG CCTCTCTGCC 
ATGGGAAATG GCCGCTCTAA TTCACCCACC AGCAGTCTTT CACAACCTAT TGAGATGCCC 
ACCCTCTCCT CCAGCCCAAC AGAGGAAAAG CCATCAGTTG GACCTGGGCA ACAGGACAAT 
CCCCTTTTGA AAACCTTTAC TAATGTCTTT GGCAGGCACC CAACTGGCTT TCTCTCCTCA 
ACAGAGTTTT TACATGATAA CAAAGCTCCT TTTGAAGCTG TAAAAAGGTT TTCACTGGAT 
GAGCGAAGTA TGACATCTAA ACAGGACTCA GATTCCAGCA CCAATAGTGA CCTGTCAGAC 
CTGAGTGACT CTGAGGAGCA GCTGCAAGCC AAGGCTGGGT TGAAGGGAAT CCCCGAGCAC 
CTAATGGGAA AGCTGGGCCC CAATGGAGAG CGAAATGCTG AACTATTACT GGGAAAAGGA 
AGAGGGAAGC AGGCTCCTAA AGGCCGACCT CGAACAGCCC CTCTGAAAGT TGGCCAATCT 
GTGCTAAAAG ACATGAGCAA GGTAAGAAAG CTCAAACAGT CAGGAGAGCC TTTCCTCCAA 
GATGGATCCT GCATTAATGT GGCCCCTCAC CTCCATAAGT GCAGGGAGTG CCGCCTGGAG 
CGCTACCGAA AGTTTAAAGA GCAAGAACAA GATGATTCCA CTGTGGCCTG CCGATTCTTT 
CACTTCCGCA GGCTTATCTT CACTCGGAAA GGCATCCTCC GTGTAGAAGG GTTTTTGAGT 
CCGCAGCAAA GTGATCCAGA AGCCATGAAT CTGTGGATTC CCTCTTCCTC CCTTGCAGAA 
GGAATCGATC TGGAGACCTC AAAATACATA CTAGCCAATG TCGGGGACCA GTTTTGCCAG 
CTTGTCATGT CAGAAAAGGA GGCCATGATG ATGGTAGAGC CACACCAAAA AGTGGCATGG 
AAACGAGCTG TACGTGGTGT CAGGGAAATG TGTGATGTAT GTGAAACAAC TCTCTTCAAC 
ATCCATTGGG TTTGTCGCAA ATGTGGATTT GGAGTCTGTC TGGACTGTTA CCGACTTAGA 
AAGAGTCGTC CACGTAGTGA GACCGAAGAG ATTGGTGATG AAGAGGTTTT CTCTTGGTTG 
AAATGTGCAA AGGGGCAGTC ACATGAACCA GAAAATCTCA TGCCTACACA AATCATCCCT 
GGCACAGCTC TTTACAATAT TGGAGATATG GTACATGCAG CCCGGGGCAA GTGGGGAATT 
AAAGCCAATT GTCCTTGCAT TAGTCGTCAG AATAAATCTG TATTGAGGCC TGCTGTCACT 
AATGGGATTT CACAGCTTCC CAGTATAAAT CCCAGTGCTT CCTCTTCTGG AAGTGAAACT 
CCCTTCTCCA GTGGAGGAGG GACAACAGGG GTGACAACAC CAGAGTCTGA CCTATTACTC 
AAACCTGACA CTCTGGACAG CAGGGGTGAG GAGGCACTGA AAACTGATGC ATCAAGTAGC 
AGTAGTGAGA CAAAAACCAG CAGGCCACTG TGCCCCGAAA CAACACCACC ATCCTCTGCT 
TTGCACTGGC TGGCAGATTT AGCAACACAG AAAGCAAAGG AAGAAACAAA AGAAGCTGGA 
TCCCTGAGAT CAGTACTCAG TAAGGAGTCT CATTCCCCCT TTGGGCTGGA TTCCTTCAAC 
TCTACAGCAA AGGTTTCTCC ATTAACTCCA AAGCTCTTTA ATAGTCTGCT ACTGGGTCCT 
GCTGCCTCAA ACAACAAAAC AGAAGGCTCC AGCCTTCGAG ACCTACTACA CTCTGGACCC 
GGAAAGCTTC CCCAGGCCCC CTTGGACACC AGCATACCTT TCCCTCCAGT CTTCTCAGCA 
TCTTCAACAG GCGTGAAGAA TAAGGCCAGT CTTCCCAACT TCCTCGACCA CATCATTGCC 
TCAGTGGTAG AAAATAAAAA AACTTCTGAT GCTGCAAAAC GGGCCAGTAA CTTGACAGAT 
ACCCAAAAAG AAGTGAAGGA GATGGTAATG GGGCTGAATG TGTTGGATCC ACACACCTCC 
CACTCATGGC TCTGTGATGG GAGGCTCCTA TGTCTCCATG ACCCCAGCAA CAAAAACAAC 
TGGAAGATCT TTCGGGAGTG TTGGAAGCAA GGCCAGCCTG TCCTTGTTTC CGGGGTGCAT 
AAGAAGCTCA AATCTGAACT TTGGAAGCCA GAAGCATTTA GTCAAGAATT TGGAGACCAG 
GATGTGGACT TAGTAAACTG TAGGAACTGT GCTATCATTT CTGATGTGAA AGTGCGTGAC 
TTCTGGGATG GTTTCGAAAT CATATGCAAA CGGCTAAGGT CAGAAGATGG GCAGCCAATG 
GTACTGAAAC TCAAAGACTG GCCTCCAGGG GAAGATTTCC GAGACATGAT GCCTACAAGG 
TTTGAGGATC TGATGGAGAA CCTCCCTCTG CCAGAGTACA CGAAGCGAGA TGGAAGGCTG 
AATTTGGCTT CTAGACTACC CAGCTATTTT GTACGACCCG ATTTGGGTCC CAAGATGTAC 
AATGCCTATG GTTTAATCAC TGCAGAAGAT AGAAGAGTTG GTACAACAAA CTTGCACTTA 
GATGTATCTG ATGCAGTTAA TGTGATGGTG TATGTTGGAA TCCCCATTGG GGAGGGAGCT 
CATGATGAAG AGGTGCTAAA GACTATTGAT GAGGGTGATG CTGATGAGGT AACAAAACAG 
CGGATTCATG AAGGGAATGA GAAACCTGGA GCGTTATGGC ATATCTATGC AGCTAAGGAT 
GCTGAGAAGA TCCGAGAACT TCTCCGAAAG GTTGGAGAAG AGCAAGGTCA AGAAAATCCT 
CCAGATCATG ATCCAATCCA TGACCAAAGC TGGTATCTGG ACCAGACTCT TCGTAAACGT 
CTCTATGAGG AGTATGGCGT GCAAGGCTGG GCAATTGTGC AGTTTCTGGG AGATGCTGTC 
TTTATACCTG CAGGAGCCCC TCACCAAGTT CATAATCTGT ACAGTTGCAT AAAAGTGGCA 
GAAGATTTTG TATCTCCTGA ACATGTAAAG CACTGTTTCC GCTTGACCCA GGAGTTCAGA 
CATCTGTCTA ACACCCACAC AAATCATGAG GATAAACTGC AGGTAAAGAA TATCATTTAC 
CATGCAGTGA AGGATGCTGT TGGCACACTG AAGGCTCATG AATCCAAACT GGCAAGATCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KDM3B ( XM_031953344.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031809204.1 Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X3, mRNA.

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CloneID	OSa225684
Clone ID Related Accession (Same CDS sequence)	XM_031953344.1
Accession Version	XM_031953344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	lysine-specific demethylase 3B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045427.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACAG AGAGCCAGAA CCAGATTCTT CTAGAACATG CTGCATTGAG AGATACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTCCAG GAAATATTCA GTCGAGGTCC CTATAGTGTC 
AAAGGCCAGA GGGTCAAAGT GTACCAGCCA GAAGGTGAAG AAGGTTGGAT CTGTGGTGTT 
GTAAGCCGAC AAGACTCTCT CACTCGTCTT ATGGAGGTAT CTTTGTCAGA GAGTGGTGAG 
ATTAAATCAG TGGATCCCAG ATTGATACAT GTGATGTTGA TGGACAACTC TGCTTCTCAG 
AATGAGGGAA GCACATTAAA AGCTGTAAAA CCCCCCAAAG GAAAGAAGAA AAGAGAGAGC 
ATAGAGGGAA AAGATGGACG AAGGAGGAAA AGTGCTTCAG ACTCTGGGTG TGACCCTGCA 
ACAAAGAAAT TAAAAGGAGA AAGGAGTGAA GTAGACAGTA ATGGGAGTGA TGGAAGTGAA 
ACAAGTCGAG GGCCCTGGAA AGGGAGTTCT AGTGGAGAGC CAGGGCTGGA TCAAAGGGCC 
AAGCAGCTGC CTCCATTTGT TCCCCAGATC AATCGAAACA TTCGATTTGC CACATACACC 
AAAGAGAATG GCAGGACTCT GGTGGTTCAG GATGAGCCTC TAGGTGGGGA CATGCCTGTA 
CCTTTCACTC CATATTCTTC AGCCACGGGG CAGTCACCTT TGGTTACAGA GGTGGGTGGA 
GCTGGAAACA AGGAGGCTGG AAAATCACTG GATTCAGGTG CTCAGAGTCT GGCAGTTTCA 
ACAGCCGTGA TCACAACCAC CAGCTCAACA CCAACCACCG TGAGAATCTC TGATACAGGC 
CTTTCAGCAG TGACCCTGCA GGAGAAACAA AAAGTCAACT GGTCTCAGAC TCAGGGAGAG 
AATTCCAGAA ACCCTCTCTT GGCTTCTTCT GGATTTGGAG CATCTCTCTC AAGTTCTTCC 
CAATCCTTGG CTTTTGGAAG TGTTAGAGCC CAGTCCAATG GGGTCTTGGC TACAGAGAGC 
AAGCCTTTGG GGTTTTCTTT TGCTTCCAGT TCTGCTACAG AAACCCAGAA AGATTCTGAT 
CTTTCAAAGA ACTTGTTTTT TCAGTGCATG TCACAGACTT TGCCCTCAAG TAACTACTTC 
ACAGCTATTT CAGAGAGTTT ATCTGATGAA TCCTCTGCTC GGGAGTCATT CAAACAGAGC 
CTAGAGAGCC TGAGCTCTGG GCTTTGTAAA GGCAAACCTA CCCTGGCAGC AGATACGAAG 
GCTGGCAGTG CCCTGGATCG AAAGATGCCT GCAGAGTCCA TGCCTACCCT CACTCCAGCC 
TTCCCAAGGA GCCTCTTGAA TACCCGAGCC CCAGAGAATC ATGAAAATCT ATTTTTGCAG 
CCCCCTAAAT TGTCCCGTGA AGAGCCATCC AACCCTTTCC TGGCATTTGC AGAGAAAGCT 
GATCTCAGTC CTTTCAGCAG TTTTACATCT CAGACAGCAG GTGGCTCCAC TTCTGCTACC 
ACTGGAGCAC TCAAAGCAGC TTCTAGCTGG CCAGAGTCTC ACACCTCTGC TGATTCAGCA 
TCTTTAGCAA AGAAGAAATC TCTCTTCATC ACAACTGATT CTTCCAAGCT AGGCTCTAGT 
GCACCTAGCT CAGCTGTGCC TCCTGGTGTC CCAAGCCTCT CTGCCATGGG AAATGGCCGC 
TCTAATTCAC CCACCAGCAG TCTTTCACAA CCTATTGAGA TGCCCACCCT CTCCTCCAGC 
CCAACAGAGG AAAAGCCATC AGTTGGACCT GGGCAACAGG ACAATCCCCT TTTGAAAACC 
TTTACTAATG TCTTTGGCAG GCACCCAACT GGCTTTCTCT CCTCAACAGA GTTTTTACAT 
GATAACAAAG CTCCTTTTGA AGCTGTAAAA AGGTTTTCAC TGGATGAGCG AAGTATGACA 
TCTAAACAGG ACTCAGATTC CAGCACCAAT AGTGACCTGT CAGACCTGAG TGACTCTGAG 
GAGCAGCTGC AAGCCAAGGC TGGGTTGAAG GGAATCCCCG AGCACCTAAT GGGAAAGCTG 
GGCCCCAATG GAGAGCGAAA TGCTGAACTA TTACTGGGAA AAGGAAGAGG GAAGCAGGCT 
CCTAAAGGCC GACCTCGAAC AGCCCCTCTG AAAGTTGGCC AATCTGTGCT AAAAGACATG 
AGCAAGGTAA GAAAGCTCAA ACAGTCAGGA GAGCCTTTCC TCCAAGATGG ATCCTGCATT 
AATGTGGCCC CTCACCTCCA TAAGTGCAGG GAGTGCCGCC TGGAGCGCTA CCGAAAGTTT 
AAAGAGCAAG AACAAGATGA TTCCACTGTG GCCTGCCGAT TCTTTCACTT CCGCAGGCTT 
ATCTTCACTC GGAAAGGCAT CCTCCGTGTA GAAGGGTTTT TGAGTCCGCA GCAAAGTGAT 
CCAGAAGCCA TGAATCTGTG GATTCCCTCT TCCTCCCTTG CAGAAGGAAT CGATCTGGAG 
ACCTCAAAAT ACATACTAGC CAATGTCGGG GACCAGTTTT GCCAGCTTGT CATGTCAGAA 
AAGGAGGCCA TGATGATGGT AGAGCCACAC CAAAAAGTGG CATGGAAACG AGCTGTACGT 
GGTGTCAGGG AAATGTGTGA TGTATGTGAA ACAACTCTCT TCAACATCCA TTGGGTTTGT 
CGCAAATGTG GATTTGGAGT CTGTCTGGAC TGTTACCGAC TTAGAAAGAG TCGTCCACGT 
AGTGAGACCG AAGAGATTGG TGATGAAGAG GTTTTCTCTT GGTTGAAATG TGCAAAGGGG 
CAGTCACATG AACCAGAAAA TCTCATGCCT ACACAAATCA TCCCTGGCAC AGCTCTTTAC 
AATATTGGAG ATATGGTACA TGCAGCCCGG GGCAAGTGGG GAATTAAAGC CAATTGTCCT 
TGCATTAGTC GTCAGAATAA ATCTGTATTG AGGCCTGCTG TCACTAATGG GATTTCACAG 
CTTCCCAGTA TAAATCCCAG TGCTTCCTCT TCTGGAAGTG AAACTCCCTT CTCCAGTGGA 
GGAGGGACAA CAGGGGTGAC AACACCAGAG TCTGACCTAT TACTCAAACC TGACACTCTG 
GACAGCAGGG GTGAGGAGGC ACTGAAAACT GATGCATCAA GTAGCAGTAG TGAGACAAAA 
ACCAGCAGGC CACTGTGCCC CGAAACAACA CCACCATCCT CTGCTTTGCA CTGGCTGGCA 
GATTTAGCAA CACAGAAAGC AAAGGAAGAA ACAAAAGAAG CTGGATCCCT GAGATCAGTA 
CTCAGTAAGG AGTCTCATTC CCCCTTTGGG CTGGATTCCT TCAACTCTAC AGCAAAGGTT 
TCTCCATTAA CTCCAAAGCT CTTTAATAGT CTGCTACTGG GTCCTGCTGC CTCAAACAAC 
AAAACAGAAG GCTCCAGCCT TCGAGACCTA CTACACTCTG GACCCGGAAA GCTTCCCCAG 
GCCCCCTTGG ACACCAGCAT ACCTTTCCCT CCAGTCTTCT CAGCATCTTC AACAGGCGTG 
AAGAATAAGG CCAGTCTTCC CAACTTCCTC GACCACATCA TTGCCTCAGT GGTAGAAAAT 
AAAAAAACTT CTGATGCTGC AAAACGGGCC AGTAACTTGA CAGATACCCA AAAAGAAGTG 
AAGGAGATGG TAATGGGGCT GAATGTGTTG GATCCACACA CCTCCCACTC ATGGCTCTGT 
GATGGGAGGC TCCTATGTCT CCATGACCCC AGCAACAAAA ACAACTGGAA GATCTTTCGG 
GAGTGTTGGA AGCAAGGCCA GATCCATAAA GAAGAGAAAG GGTGCAGGAT TTCTGGAGTT 
ATCCTTTTGA AATATGAAAT GTCACCAGAA GTTGATTTTT TTCAGTGTTT GGCCTTCCAC 
TTTCTTTACA TAGTACCTGT CCTTGTTTCC GGGGTGCATA AGAAGCTCAA ATCTGAACTT 
TGGAAGCCAG AAGCATTTAG TCAAGAATTT GGAGACCAGG ATGTGGACTT AGTAAACTGT 
AGGAACTGTG CTATCATTTC TGATGTGAAA GTGCGTGACT TCTGGGATGG TTTCGAAATC 
ATATGCAAAC GGCTAAGGTC AGAAGATGGG CAGCCAATGG TACTGAAACT CAAAGACTGG 
CCTCCAGGGG AAGATTTCCG AGACATGATG CCTACAAGGT TTGAGGATCT GATGGAGAAC 
CTCCCTCTGC CAGAGTACAC GAAGCGAGAT GGAAGGCTGA ATTTGGCTTC TAGACTACCC 
AGCTATTTTG TACGACCCGA TTTGGGTCCC AAGATGTACA ATGCCTATGG TTTAATCACT 
GCAGAAGATA GAAGAGTTGG TACAACAAAC TTGCACTTAG ATGTATCTGA TGCAGTTAAT 
GTGATGGTGT ATGTTGGAAT CCCCATTGGG GAGGGAGCTC ATGATGAAGA GGTGCTAAAG 
ACTATTGATG AGGGTGATGC TGATGAGGTA ACAAAACAGC GGATTCATGA AGGGAATGAG 
AAACCTGGAG CGTTATGGCA TATCTATGCA GCTAAGGATG CTGAGAAGAT CCGAGAACTT 
CTCCGAAAGG TTGGAGAAGA GCAAGGTCAA GAAAATCCTC CAGATCATGA TCCAATCCAT 
GACCAAAGCT GGTATCTGGA CCAGACTCTT CGTAAACGTC TCTATGAGGA GTATGGCGTG 
CAAGGCTGGG CAATTGTGCA GTTTCTGGGA GATGCTGTCT TTATACCTGC AGGAGCCCCT 
CACCAAGTTC ATAATCTGTA CAGTTGCATA AAAGTGGCAG AAGATTTTGT ATCTCCTGAA 
CATGTAAAGC ACTGTTTCCG CTTGACCCAG GAGTTCAGAC ATCTGTCTAA CACCCACACA 
AATCATGAGG ATAAACTGCA GGTAAAGAAT ATCATTTACC ATGCAGTGAA GGATGCTGTT 
GGCACACTGA AGGCTCATGA ATCCAAACTG GCAAGATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031809204.1
CDS	354..5255
Translation	MGTESQNQILLEHAALRDTVNALISDQKLQEIFSRGPYSVKGQRVKVYQPEGEEGWICGVVSRQDSLTRLMEVSLSESGEIKSVDPRLIHVMLMDNSASQNEGSTLKAVKPPKGKKKRESIEGKDGRRRKSASDSGCDPATKKLKGERSEVDSNGSDGSETSRGPWKGSSSGEPGLDQRAKQLPPFVPQINRNIRFATYTKENGRTLVVQDEPLGGDMPVPFTPYSSATGQSPLVTEVGGAGNKEAGKSLDSGAQSLAVSTAVITTTSSTPTTVRISDTGLSAVTLQEKQKVNWSQTQGENSRNPLLASSGFGASLSSSSQSLAFGSVRAQSNGVLATESKPLGFSFASSSATETQKDSDLSKNLFFQCMSQTLPSSNYFTAISESLSDESSARESFKQSLESLSSGLCKGKPTLAADTKAGSALDRKMPAESMPTLTPAFPRSLLNTRAPENHENLFLQPPKLSREEPSNPFLAFAEKADLSPFSSFTSQTAGGSTSATTGALKAASSWPESHTSADSASLAKKKSLFITTDSSKLGSSAPSSAVPPGVPSLSAMGNGRSNSPTSSLSQPIEMPTLSSSPTEEKPSVGPGQQDNPLLKTFTNVFGRHPTGFLSSTEFLHDNKAPFEAVKRFSLDERSMTSKQDSDSSTNSDLSDLSDSEEQLQAKAGLKGIPEHLMGKLGPNGERNAELLLGKGRGKQAPKGRPRTAPLKVGQSVLKDMSKVRKLKQSGEPFLQDGSCINVAPHLHKCRECRLERYRKFKEQEQDDSTVACRFFHFRRLIFTRKGILRVEGFLSPQQSDPEAMNLWIPSSSLAEGIDLETSKYILANVGDQFCQLVMSEKEAMMMVEPHQKVAWKRAVRGVREMCDVCETTLFNIHWVCRKCGFGVCLDCYRLRKSRPRSETEEIGDEEVFSWLKCAKGQSHEPENLMPTQIIPGTALYNIGDMVHAARGKWGIKANCPCISRQNKSVLRPAVTNGISQLPSINPSASSSGSETPFSSGGGTTGVTTPESDLLLKPDTLDSRGEEALKTDASSSSSETKTSRPLCPETTPPSSALHWLADLATQKAKEETKEAGSLRSVLSKESHSPFGLDSFNSTAKVSPLTPKLFNSLLLGPAASNNKTEGSSLRDLLHSGPGKLPQAPLDTSIPFPPVFSASSTGVKNKASLPNFLDHIIASVVENKKTSDAAKRASNLTDTQKEVKEMVMGLNVLDPHTSHSWLCDGRLLCLHDPSNKNNWKIFRECWKQGQIHKEEKGCRISGVILLKYEMSPEVDFFQCLAFHFLYIVPVLVSGVHKKLKSELWKPEAFSQEFGDQDVDLVNCRNCAIISDVKVRDFWDGFEIICKRLRSEDGQPMVLKLKDWPPGEDFRDMMPTRFEDLMENLPLPEYTKRDGRLNLASRLPSYFVRPDLGPKMYNAYGLITAEDRRVGTTNLHLDVSDAVNVMVYVGIPIGEGAHDEEVLKTIDEGDADEVTKQRIHEGNEKPGALWHIYAAKDAEKIRELLRKVGEEQGQENPPDHDPIHDQSWYLDQTLRKRLYEEYGVQGWAIVQFLGDAVFIPAGAPHQVHNLYSCIKVAEDFVSPEHVKHCFRLTQEFRHLSNTHTNHEDKLQVKNIIYHAVKDAVGTLKAHESKLARS

Target ORF information:

RefSeq Version	XM_031953344.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii lysine demethylase 3B (KDM3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031953344.1

ORF Insert Sequence:

ATGGGAACAG AGAGCCAGAA CCAGATTCTT CTAGAACATG CTGCATTGAG AGATACAGTT 
AATGCTTTGA TCAGTGACCA AAAGCTCCAG GAAATATTCA GTCGAGGTCC CTATAGTGTC 
AAAGGCCAGA GGGTCAAAGT GTACCAGCCA GAAGGTGAAG AAGGTTGGAT CTGTGGTGTT 
GTAAGCCGAC AAGACTCTCT CACTCGTCTT ATGGAGGTAT CTTTGTCAGA GAGTGGTGAG 
ATTAAATCAG TGGATCCCAG ATTGATACAT GTGATGTTGA TGGACAACTC TGCTTCTCAG 
AATGAGGGAA GCACATTAAA AGCTGTAAAA CCCCCCAAAG GAAAGAAGAA AAGAGAGAGC 
ATAGAGGGAA AAGATGGACG AAGGAGGAAA AGTGCTTCAG ACTCTGGGTG TGACCCTGCA 
ACAAAGAAAT TAAAAGGAGA AAGGAGTGAA GTAGACAGTA ATGGGAGTGA TGGAAGTGAA 
ACAAGTCGAG GGCCCTGGAA AGGGAGTTCT AGTGGAGAGC CAGGGCTGGA TCAAAGGGCC 
AAGCAGCTGC CTCCATTTGT TCCCCAGATC AATCGAAACA TTCGATTTGC CACATACACC 
AAAGAGAATG GCAGGACTCT GGTGGTTCAG GATGAGCCTC TAGGTGGGGA CATGCCTGTA 
CCTTTCACTC CATATTCTTC AGCCACGGGG CAGTCACCTT TGGTTACAGA GGTGGGTGGA 
GCTGGAAACA AGGAGGCTGG AAAATCACTG GATTCAGGTG CTCAGAGTCT GGCAGTTTCA 
ACAGCCGTGA TCACAACCAC CAGCTCAACA CCAACCACCG TGAGAATCTC TGATACAGGC 
CTTTCAGCAG TGACCCTGCA GGAGAAACAA AAAGTCAACT GGTCTCAGAC TCAGGGAGAG 
AATTCCAGAA ACCCTCTCTT GGCTTCTTCT GGATTTGGAG CATCTCTCTC AAGTTCTTCC 
CAATCCTTGG CTTTTGGAAG TGTTAGAGCC CAGTCCAATG GGGTCTTGGC TACAGAGAGC 
AAGCCTTTGG GGTTTTCTTT TGCTTCCAGT TCTGCTACAG AAACCCAGAA AGATTCTGAT 
CTTTCAAAGA ACTTGTTTTT TCAGTGCATG TCACAGACTT TGCCCTCAAG TAACTACTTC 
ACAGCTATTT CAGAGAGTTT ATCTGATGAA TCCTCTGCTC GGGAGTCATT CAAACAGAGC 
CTAGAGAGCC TGAGCTCTGG GCTTTGTAAA GGCAAACCTA CCCTGGCAGC AGATACGAAG 
GCTGGCAGTG CCCTGGATCG AAAGATGCCT GCAGAGTCCA TGCCTACCCT CACTCCAGCC 
TTCCCAAGGA GCCTCTTGAA TACCCGAGCC CCAGAGAATC ATGAAAATCT ATTTTTGCAG 
CCCCCTAAAT TGTCCCGTGA AGAGCCATCC AACCCTTTCC TGGCATTTGC AGAGAAAGCT 
GATCTCAGTC CTTTCAGCAG TTTTACATCT CAGACAGCAG GTGGCTCCAC TTCTGCTACC 
ACTGGAGCAC TCAAAGCAGC TTCTAGCTGG CCAGAGTCTC ACACCTCTGC TGATTCAGCA 
TCTTTAGCAA AGAAGAAATC TCTCTTCATC ACAACTGATT CTTCCAAGCT AGGCTCTAGT 
GCACCTAGCT CAGCTGTGCC TCCTGGTGTC CCAAGCCTCT CTGCCATGGG AAATGGCCGC 
TCTAATTCAC CCACCAGCAG TCTTTCACAA CCTATTGAGA TGCCCACCCT CTCCTCCAGC 
CCAACAGAGG AAAAGCCATC AGTTGGACCT GGGCAACAGG ACAATCCCCT TTTGAAAACC 
TTTACTAATG TCTTTGGCAG GCACCCAACT GGCTTTCTCT CCTCAACAGA GTTTTTACAT 
GATAACAAAG CTCCTTTTGA AGCTGTAAAA AGGTTTTCAC TGGATGAGCG AAGTATGACA 
TCTAAACAGG ACTCAGATTC CAGCACCAAT AGTGACCTGT CAGACCTGAG TGACTCTGAG 
GAGCAGCTGC AAGCCAAGGC TGGGTTGAAG GGAATCCCCG AGCACCTAAT GGGAAAGCTG 
GGCCCCAATG GAGAGCGAAA TGCTGAACTA TTACTGGGAA AAGGAAGAGG GAAGCAGGCT 
CCTAAAGGCC GACCTCGAAC AGCCCCTCTG AAAGTTGGCC AATCTGTGCT AAAAGACATG 
AGCAAGGTAA GAAAGCTCAA ACAGTCAGGA GAGCCTTTCC TCCAAGATGG ATCCTGCATT 
AATGTGGCCC CTCACCTCCA TAAGTGCAGG GAGTGCCGCC TGGAGCGCTA CCGAAAGTTT 
AAAGAGCAAG AACAAGATGA TTCCACTGTG GCCTGCCGAT TCTTTCACTT CCGCAGGCTT 
ATCTTCACTC GGAAAGGCAT CCTCCGTGTA GAAGGGTTTT TGAGTCCGCA GCAAAGTGAT 
CCAGAAGCCA TGAATCTGTG GATTCCCTCT TCCTCCCTTG CAGAAGGAAT CGATCTGGAG 
ACCTCAAAAT ACATACTAGC CAATGTCGGG GACCAGTTTT GCCAGCTTGT CATGTCAGAA 
AAGGAGGCCA TGATGATGGT AGAGCCACAC CAAAAAGTGG CATGGAAACG AGCTGTACGT 
GGTGTCAGGG AAATGTGTGA TGTATGTGAA ACAACTCTCT TCAACATCCA TTGGGTTTGT 
CGCAAATGTG GATTTGGAGT CTGTCTGGAC TGTTACCGAC TTAGAAAGAG TCGTCCACGT 
AGTGAGACCG AAGAGATTGG TGATGAAGAG GTTTTCTCTT GGTTGAAATG TGCAAAGGGG 
CAGTCACATG AACCAGAAAA TCTCATGCCT ACACAAATCA TCCCTGGCAC AGCTCTTTAC 
AATATTGGAG ATATGGTACA TGCAGCCCGG GGCAAGTGGG GAATTAAAGC CAATTGTCCT 
TGCATTAGTC GTCAGAATAA ATCTGTATTG AGGCCTGCTG TCACTAATGG GATTTCACAG 
CTTCCCAGTA TAAATCCCAG TGCTTCCTCT TCTGGAAGTG AAACTCCCTT CTCCAGTGGA 
GGAGGGACAA CAGGGGTGAC AACACCAGAG TCTGACCTAT TACTCAAACC TGACACTCTG 
GACAGCAGGG GTGAGGAGGC ACTGAAAACT GATGCATCAA GTAGCAGTAG TGAGACAAAA 
ACCAGCAGGC CACTGTGCCC CGAAACAACA CCACCATCCT CTGCTTTGCA CTGGCTGGCA 
GATTTAGCAA CACAGAAAGC AAAGGAAGAA ACAAAAGAAG CTGGATCCCT GAGATCAGTA 
CTCAGTAAGG AGTCTCATTC CCCCTTTGGG CTGGATTCCT TCAACTCTAC AGCAAAGGTT 
TCTCCATTAA CTCCAAAGCT CTTTAATAGT CTGCTACTGG GTCCTGCTGC CTCAAACAAC 
AAAACAGAAG GCTCCAGCCT TCGAGACCTA CTACACTCTG GACCCGGAAA GCTTCCCCAG 
GCCCCCTTGG ACACCAGCAT ACCTTTCCCT CCAGTCTTCT CAGCATCTTC AACAGGCGTG 
AAGAATAAGG CCAGTCTTCC CAACTTCCTC GACCACATCA TTGCCTCAGT GGTAGAAAAT 
AAAAAAACTT CTGATGCTGC AAAACGGGCC AGTAACTTGA CAGATACCCA AAAAGAAGTG 
AAGGAGATGG TAATGGGGCT GAATGTGTTG GATCCACACA CCTCCCACTC ATGGCTCTGT 
GATGGGAGGC TCCTATGTCT CCATGACCCC AGCAACAAAA ACAACTGGAA GATCTTTCGG 
GAGTGTTGGA AGCAAGGCCA GATCCATAAA GAAGAGAAAG GGTGCAGGAT TTCTGGAGTT 
ATCCTTTTGA AATATGAAAT GTCACCAGAA GTTGATTTTT TTCAGTGTTT GGCCTTCCAC 
TTTCTTTACA TAGTACCTGT CCTTGTTTCC GGGGTGCATA AGAAGCTCAA ATCTGAACTT 
TGGAAGCCAG AAGCATTTAG TCAAGAATTT GGAGACCAGG ATGTGGACTT AGTAAACTGT 
AGGAACTGTG CTATCATTTC TGATGTGAAA GTGCGTGACT TCTGGGATGG TTTCGAAATC 
ATATGCAAAC GGCTAAGGTC AGAAGATGGG CAGCCAATGG TACTGAAACT CAAAGACTGG 
CCTCCAGGGG AAGATTTCCG AGACATGATG CCTACAAGGT TTGAGGATCT GATGGAGAAC 
CTCCCTCTGC CAGAGTACAC GAAGCGAGAT GGAAGGCTGA ATTTGGCTTC TAGACTACCC 
AGCTATTTTG TACGACCCGA TTTGGGTCCC AAGATGTACA ATGCCTATGG TTTAATCACT 
GCAGAAGATA GAAGAGTTGG TACAACAAAC TTGCACTTAG ATGTATCTGA TGCAGTTAAT 
GTGATGGTGT ATGTTGGAAT CCCCATTGGG GAGGGAGCTC ATGATGAAGA GGTGCTAAAG 
ACTATTGATG AGGGTGATGC TGATGAGGTA ACAAAACAGC GGATTCATGA AGGGAATGAG 
AAACCTGGAG CGTTATGGCA TATCTATGCA GCTAAGGATG CTGAGAAGAT CCGAGAACTT 
CTCCGAAAGG TTGGAGAAGA GCAAGGTCAA GAAAATCCTC CAGATCATGA TCCAATCCAT 
GACCAAAGCT GGTATCTGGA CCAGACTCTT CGTAAACGTC TCTATGAGGA GTATGGCGTG 
CAAGGCTGGG CAATTGTGCA GTTTCTGGGA GATGCTGTCT TTATACCTGC AGGAGCCCCT 
CACCAAGTTC ATAATCTGTA CAGTTGCATA AAAGTGGCAG AAGATTTTGT ATCTCCTGAA 
CATGTAAAGC ACTGTTTCCG CTTGACCCAG GAGTTCAGAC ATCTGTCTAA CACCCACACA 
AATCATGAGG ATAAACTGCA GGTAAAGAAT ATCATTTACC ATGCAGTGAA GGATGCTGTT 
GGCACACTGA AGGCTCATGA ATCCAAACTG GCAAGATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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KDM3B cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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