PTCH2 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTCH2
Entrez Gene ID	100916643
Full Name	patched 2
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031969231.1	XP_031825091.1	protein patched homolog 2 isoform X1
XM_031969232.1	XP_031825092.1	protein patched homolog 2 isoform X2
XM_012547495.1	XP_012402949.1	protein patched homolog 2
XM_012547495.2	XP_012402949.1	protein patched homolog 2

Pathways from BioSystems
Gene Ontology

The following PTCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa235794	XM_031969232.1 Latest version!	Sarcophilus harrisii patched 2 (PTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OSa235793	XM_031969231.1 Latest version!	Sarcophilus harrisii patched 2 (PTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa129083	XM_012547495.2 Latest version!	Sarcophilus harrisii patched 2 (PTCH2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OSa129083	XM_012547495.1	Sarcophilus harrisii patched 2 (PTCH2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTCH2 ( XM_031969232.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031825092.1 Sarcophilus harrisii patched 2 (PTCH2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa235794
Clone ID Related Accession (Same CDS sequence)	XM_031969232.1
Accession Version	XM_031969232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein patched homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCGG GGGAGCTGCT GACCTCCGGC CCCGCGCCCC CCGCGCAGGG CCGGGCCCCC 
CTCTGGTTGC GAGCCCACGT GGGCGGCTTG CTCTTCAGCT TGGGCTGCGC GATCCAGCGG 
CATTGTGGCA AGGTGCTCTT CGTGGGGCTG CTGGCTTTCG GGGCCCTTGC GCTGGGCCTG 
CGTGGGGCTG CCGTGGAGAC AGACCTGGAG CGCCTCTGGG TGGAAGTGGG CAGCCGGGTG 
AGCCAGGAGC TCCGTTACAC AAAGGAGAAG CTCGGGGAAG AGGCCGTGTA CACCTCTCAG 
ACCCTCATCC AGACCGCCCG TCATCCGGGC GAGAGCGTTC TCACACCCGA GGCTCTGGGC 
CTCCACCTTC AGGCTGCCTT GGCAGCTAGC AAAGTTCAAG TTTCCCTCTA TGGAAAGTCC 
TGGGATCTGA ACAAAATCTG CTACAAGGCA GGAGTCCCTG TTATTGAGAA TGGGATGATT 
GAGCGGATGA TCGAGAAGCT GTTTCCCTGT GTGATCCTCA CCCCGCTGGA CTGCTTTTGG 
GAGGGAGCCA AACTTCAGGG GGGCTCCGCG TACCTACCGG GCCGACCTGA TATTCAGTGG 
ACCAACCTGG ATCCTGAGCA ACTTCTGGAA GAACTCGGCC CCTTTGCCTC CCTGGAGGGC 
TTTAGGGAGC TGCTGGACAA GGCCCAGGTG GGCCAGGCCT ACGTGGGACG GCCCTGCCTG 
CATCCCAGTG ATCCCCATTG CCCAGCCAGT GCCCCTAATC GCCAGAGCAG GCAGGTCCCA 
GATGTTGCCC GGGAGCTGAG CGGCGGCTGC CATGGCTTCT CACGCAAGTT TATGCATTGG 
CAGGAGGAGC TGCTGCTGGG GAGCCCGTGC GGAAGCCCTG CAGAGCACCT TCCTGCTCAT 
GAGCCCCCGG CAGCTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031825092.1
CDS	137..1057
Translation	MAPGELLTSGPAPPAQGRAPLWLRAHVGGLLFSLGCAIQRHCGKVLFVGLLAFGALALGLRGAAVETDLERLWVEVGSRVSQELRYTKEKLGEEAVYTSQTLIQTARHPGESVLTPEALGLHLQAALAASKVQVSLYGKSWDLNKICYKAGVPVIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPSDPHCPASAPNRQSRQVPDVARELSGGCHGFSRKFMHWQEELLLGSPCGSPAEHLPAHEPPAAV

Target ORF information:

RefSeq Version	XM_031969232.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii patched 2 (PTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031969232.1

ORF Insert Sequence:

ATGGCCCCGG GGGAGCTGCT GACCTCCGGC CCCGCGCCCC CCGCGCAGGG CCGGGCCCCC 
CTCTGGTTGC GAGCCCACGT GGGCGGCTTG CTCTTCAGCT TGGGCTGCGC GATCCAGCGG 
CATTGTGGCA AGGTGCTCTT CGTGGGGCTG CTGGCTTTCG GGGCCCTTGC GCTGGGCCTG 
CGTGGGGCTG CCGTGGAGAC AGACCTGGAG CGCCTCTGGG TGGAAGTGGG CAGCCGGGTG 
AGCCAGGAGC TCCGTTACAC AAAGGAGAAG CTCGGGGAAG AGGCCGTGTA CACCTCTCAG 
ACCCTCATCC AGACCGCCCG TCATCCGGGC GAGAGCGTTC TCACACCCGA GGCTCTGGGC 
CTCCACCTTC AGGCTGCCTT GGCAGCTAGC AAAGTTCAAG TTTCCCTCTA TGGAAAGTCC 
TGGGATCTGA ACAAAATCTG CTACAAGGCA GGAGTCCCTG TTATTGAGAA TGGGATGATT 
GAGCGGATGA TCGAGAAGCT GTTTCCCTGT GTGATCCTCA CCCCGCTGGA CTGCTTTTGG 
GAGGGAGCCA AACTTCAGGG GGGCTCCGCG TACCTACCGG GCCGACCTGA TATTCAGTGG 
ACCAACCTGG ATCCTGAGCA ACTTCTGGAA GAACTCGGCC CCTTTGCCTC CCTGGAGGGC 
TTTAGGGAGC TGCTGGACAA GGCCCAGGTG GGCCAGGCCT ACGTGGGACG GCCCTGCCTG 
CATCCCAGTG ATCCCCATTG CCCAGCCAGT GCCCCTAATC GCCAGAGCAG GCAGGTCCCA 
GATGTTGCCC GGGAGCTGAG CGGCGGCTGC CATGGCTTCT CACGCAAGTT TATGCATTGG 
CAGGAGGAGC TGCTGCTGGG GAGCCCGTGC GGAAGCCCTG CAGAGCACCT TCCTGCTCAT 
GAGCCCCCGG CAGCTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_031969231.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031825091.1 Sarcophilus harrisii patched 2 (PTCH2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa235793
Clone ID Related Accession (Same CDS sequence)	XM_031969231.1
Accession Version	XM_031969231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein patched homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCGG GGGAGCTGCT GACCTCCGGC CCCGCGCCCC CCGCGCAGGG CCGGGCCCCC 
CTCTGGTTGC GAGCCCACGT GGGCGGCTTG CTCTTCAGCT TGGGCTGCGC GATCCAGCGG 
CATTGTGGCA AGGTGCTCTT CGTGGGGCTG CTGGCTTTCG GGGCCCTTGC GCTGGGCCTG 
CGTGGGGCTG CCGTGGAGAC AGACCTGGAG CGCCTCTGGG TGGAAGTGGG CAGCCGGGTG 
AGCCAGGAGC TCCGTTACAC AAAGGAGAAG CTCGGGGAAG AGGCCGTGTA CACCTCTCAG 
ACCCTCATCC AGACCGCCCG TCATCCGGGC GAGAGCGTTC TCACACCCGA GGCTCTGGGC 
CTCCACCTTC AGGCTGCCTT GGCAGCTAGC AAAGTTCAAG TTTCCCTCTA TGGAAAGTCC 
TGGGATCTGA ACAAAATCTG CTACAAGGCA GGAGTCCCTG TTATTGAGAA TGGGATGATT 
GAGCGGATGA TCGAGAAGCT GTTTCCCTGT GTGATCCTCA CCCCGCTGGA CTGCTTTTGG 
GAGGGAGCCA AACTTCAGGG GGGCTCCGCG TACCTACCGG GCCGACCTGA TATTCAGTGG 
ACCAACCTGG ATCCTGAGCA ACTTCTGGAA GAACTCGGCC CCTTTGCCTC CCTGGAGGGC 
TTTAGGGAGC TGCTGGACAA GGCCCAGGTG GGCCAGGCCT ACGTGGGACG GCCCTGCCTG 
CATCCCAGTG ATCCCCATTG CCCAGCCAGT GCCCCTAATC GCCAGAGCAG GCAGGTCCCA 
GATGTTGCCC GGGAGCTGAG CGGCGGCTGC CATGGCTTCT CACGCAAGTT TATGCATTGG 
CAGGAGGAGC TGCTGCTGGG GAGCCCGGTG AGGAGCCCTC AGGGCCGGCT GCTCAGTGCG 
GAAGCCCTGC AGAGCACCTT CCTGCTCATG AGCCCCCGGC AGCTGTATGA CCATTACCGA 
GGAGACTATG AAACCCATGA CATCAGCTGG AGCGAGTCCC AGGCAGGCGC TGTGCTGCAG 
GCCTGGCAGA GGCGCTTCGT GGAGCTGGCC CAGCAGGCCA TCCCCCAGAA TGCGTCCCAG 
CAGATCCACG CCTTCTCTGC CACCACCCTG GATGACCTCT TGCGCTCCTT CTCTGACATC 
AGTGCAGTCC GTGTGGCGGG GGGCTACCTC CTCATGCTGG CTTACGCCTG CGTGACCATG 
CTCCGCTGGG ACTGCTCCAA GTCTCAGGGG GCCGTGGGCC TGGCCGGGGT CCTTCTGGTA 
GCCCTGTCTG TGGCCTCTGG CCTCGGGCTC TGCTCGCTGC TGGGTATGAC CTTCAATGCT 
GCTACGACTC AGGTGCTCCC ATTCCTGGCC CTGGGCATCG GGGTGGACGA CATGTTCCTG 
CTGGCCCACG CCTTCACCGA GGCCCCCTCG GGCACCCCCC TGCAGGAGCG GACCGGCGAG 
TGCCTGCAGC GCATGGGCAC CAGCGTGGTG CTCACCTCCG TCAACAACCT GGTCGCCTTC 
TTCATGGCCG CCCTGGTGCC CATCCCCGCC CTTCGCGCCT TCTCCCTCCA GGCTGCGGTG 
GTGGTCGGCT GCAACTTTGC TGCTGTGCTG CTCATCTTCC CGGCCATCCT CAGCTTGGAC 
CTGCACCGGC GGCACCGGCA GCGGCTGGAT GTGCTCTGCT GCTTCTCCAG CCCATGTTCT 
TCCCGGGTGA TTCAGATCCA GCCTCAGGAG CTGGGGGAGG GGCAGGTGCC CAGGAGCGGG 
GCCCACCTGA CAGCCACGGT CCAGGCCTTC GCCCGCTGCG ACACAGCCGG CCAGCACGTC 
GTCACCATCC TGCCCCCCAC CACTCACCTG ACGTCCCTCC CCACCGAGTC TCTGGGCTCC 
CAGCTCTTTG GCCCCATGGG TTCAACCAGA GACTTGCTGG GCCAGGTGCC CGGGCCGGGC 
CGGGGCCAGG TGTGCCGCCC GCTGCCCTGT GCCCGCTGGA ACCTCTCTCG CTTTGCCCGG 
TGCCAGTACG CCCCCCTGCT GTTGCAGCCC CGAACCAAGG GCCTGGTACT GGTGCTTTTC 
GGGGCCCTCC TGGGGCTGAG CCTGTACGGA GCCACGTGGG TGCAGGATGG GCTGACCCTG 
ACGGATGTGG TGCCCCGGGG CACCAAGGAG TACGACTTCC TGGCCGCCCA GATCAAGTAC 
TTCTCCCTGT ACGAGGTGGC CCTTGTGACC CAGGGTGGCT TCGACTACGC CCGCTCCCAG 
CAGGCCCTGC TGGACCTGCA CAAACGCTTC AGCGCCCTCA AATCCGTGCT GGCGCCGCAG 
CCACCGAGAA GCTGGCTACA CCGCTACAGC GCCTGGCTCC AGGGGCTCCA GGCCGCCTTT 
GACCAAGACT GGGCCGCCGG CCGCATCACG CGCCACTCGT GCCGCAACGG CTCCGAGGAT 
GGAGCCCTGG CCTACAGGCT GCTCATCCAG ACGGGGGACG CCAAGGAGCC GCTGGACTAC 
AGCCAGCTGG ACAAGCGGAA ACTGGTGGAT GCTGATGGCC TCATCCTGCC CGAGCTCTTC 
TACGTTGGGC TGACCGTGTG GGTGAGCAGA GACCCTTTGG GCCTGGCAGC CTCCCAGGCC 
AACTTCTACC CCCCGCCCCC CGAGTGGCTC CACGACAAAT ATGACAGCAC TGGGGAGAGC 
TTGCACATCC CTGCGGCCCA GCCTTTGGAG TTTGCCCAGT TCCCCTTCCT GCTGAGCGGG 
CTCCGCCAGA CGGCAGACTT CGTGGAGGCC ATCGAGGGGG CCCGGGCGGC CTGCGCAGAG 
GCGGCCCGCG CTGGGATCCG GGCCTACCCC AGCGGCTCCC CGTTCCTCTT CTGGGAGCAG 
TACTTGGGCC TCCGCCGCTG CTTTCTGCTG GCCGTCTGCG TGCTGCTGGC CTGCACCTTC 
GTGGTCTGTG CCTTGCTGCT GCTCAGCCCC TGGACCGCCG GCCTCATTGT GCTGGTGCTG 
GCCATGATGA CTGTGGAATT ATTCGGCATC ATGGGCTTCC TGGGCATCAA ACTCAGCGCC 
ATCCCCGTGG TGATTCTCGT GGCCTCCGTG GGCATTGGCG TCGAGTTCAC AGCTCACGTG 
GCTCTGGGCT TCCTGACAGC CACCGGCAGC CGGGACGCGC GGTCAGCCCA GGCCTTGGAG 
CACATGTTTG CGCCCGTGAT GGATGGGGCC GTGTCCACGC TGCTGGGCCT GCTCATGCTG 
GCCGGCTCGG ACTTCGACTT TATTGTCAGG TACTTCTTTG TGGTGCTGAC AGTGCTGACC 
GGCCTGGGCC TGCTTCACGG GCTTGTTCTG CTTCCTGTGC TGCTGTCCAT CATAGGACCC 
CCACCCCAGG TACCGCTGCC AGATGGAGGC GGCCGCCCGC CCTGCCCTGA CCCGGTATCG 
CTGCCCCTCA GTCCTCCTCA CTTCTTCCTG GGCCCGAGCC CAGCCTTCAG GGCTCCTGAG 
ACAGGAGCAG GGGACGCCCC AAGCACCTTC ATCCTGCCCC CGGCCCACTC CCACATCTTG 
GTGGAGGCCA GCAAGGACCC CAGCTTCCCG ACCATCACGG TGGTGCAGAC GTACAAGGAT 
GGTTCTCCTG GCTCCGGGCT ACCTCCCCTT ACGGCCACGG CCGGCAGTGA GGCCCGCTGG 
GGGCCCCATG CCAGCCCCGG CGCCTTCACC ACCGTGACGG CCTCCGTCAC CGTGGCCCTG 
CACCCGCCGC CACTGCCGGG TAGCTACGTC CAGGAGGTCC CTGAGGAAGC CAGGCCCCCG 
ATGGCCGGCG AGTCCAAGGG ACCTGGCCCC CGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031825091.1
CDS	136..3894
Translation	MAPGELLTSGPAPPAQGRAPLWLRAHVGGLLFSLGCAIQRHCGKVLFVGLLAFGALALGLRGAAVETDLERLWVEVGSRVSQELRYTKEKLGEEAVYTSQTLIQTARHPGESVLTPEALGLHLQAALAASKVQVSLYGKSWDLNKICYKAGVPVIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPSDPHCPASAPNRQSRQVPDVARELSGGCHGFSRKFMHWQEELLLGSPVRSPQGRLLSAEALQSTFLLMSPRQLYDHYRGDYETHDISWSESQAGAVLQAWQRRFVELAQQAIPQNASQQIHAFSATTLDDLLRSFSDISAVRVAGGYLLMLAYACVTMLRWDCSKSQGAVGLAGVLLVALSVASGLGLCSLLGMTFNAATTQVLPFLALGIGVDDMFLLAHAFTEAPSGTPLQERTGECLQRMGTSVVLTSVNNLVAFFMAALVPIPALRAFSLQAAVVVGCNFAAVLLIFPAILSLDLHRRHRQRLDVLCCFSSPCSSRVIQIQPQELGEGQVPRSGAHLTATVQAFARCDTAGQHVVTILPPTTHLTSLPTESLGSQLFGPMGSTRDLLGQVPGPGRGQVCRPLPCARWNLSRFARCQYAPLLLQPRTKGLVLVLFGALLGLSLYGATWVQDGLTLTDVVPRGTKEYDFLAAQIKYFSLYEVALVTQGGFDYARSQQALLDLHKRFSALKSVLAPQPPRSWLHRYSAWLQGLQAAFDQDWAAGRITRHSCRNGSEDGALAYRLLIQTGDAKEPLDYSQLDKRKLVDADGLILPELFYVGLTVWVSRDPLGLAASQANFYPPPPEWLHDKYDSTGESLHIPAAQPLEFAQFPFLLSGLRQTADFVEAIEGARAACAEAARAGIRAYPSGSPFLFWEQYLGLRRCFLLAVCVLLACTFVVCALLLLSPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTAHVALGFLTATGSRDARSAQALEHMFAPVMDGAVSTLLGLLMLAGSDFDFIVRYFFVVLTVLTGLGLLHGLVLLPVLLSIIGPPPQVPLPDGGGRPPCPDPVSLPLSPPHFFLGPSPAFRAPETGAGDAPSTFILPPAHSHILVEASKDPSFPTITVVQTYKDGSPGSGLPPLTATAGSEARWGPHASPGAFTTVTASVTVALHPPPLPGSYVQEVPEEARPPMAGESKGPGPRC

Target ORF information:

RefSeq Version	XM_031969231.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii patched 2 (PTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031969231.1

ORF Insert Sequence:

ATGGCCCCGG GGGAGCTGCT GACCTCCGGC CCCGCGCCCC CCGCGCAGGG CCGGGCCCCC 
CTCTGGTTGC GAGCCCACGT GGGCGGCTTG CTCTTCAGCT TGGGCTGCGC GATCCAGCGG 
CATTGTGGCA AGGTGCTCTT CGTGGGGCTG CTGGCTTTCG GGGCCCTTGC GCTGGGCCTG 
CGTGGGGCTG CCGTGGAGAC AGACCTGGAG CGCCTCTGGG TGGAAGTGGG CAGCCGGGTG 
AGCCAGGAGC TCCGTTACAC AAAGGAGAAG CTCGGGGAAG AGGCCGTGTA CACCTCTCAG 
ACCCTCATCC AGACCGCCCG TCATCCGGGC GAGAGCGTTC TCACACCCGA GGCTCTGGGC 
CTCCACCTTC AGGCTGCCTT GGCAGCTAGC AAAGTTCAAG TTTCCCTCTA TGGAAAGTCC 
TGGGATCTGA ACAAAATCTG CTACAAGGCA GGAGTCCCTG TTATTGAGAA TGGGATGATT 
GAGCGGATGA TCGAGAAGCT GTTTCCCTGT GTGATCCTCA CCCCGCTGGA CTGCTTTTGG 
GAGGGAGCCA AACTTCAGGG GGGCTCCGCG TACCTACCGG GCCGACCTGA TATTCAGTGG 
ACCAACCTGG ATCCTGAGCA ACTTCTGGAA GAACTCGGCC CCTTTGCCTC CCTGGAGGGC 
TTTAGGGAGC TGCTGGACAA GGCCCAGGTG GGCCAGGCCT ACGTGGGACG GCCCTGCCTG 
CATCCCAGTG ATCCCCATTG CCCAGCCAGT GCCCCTAATC GCCAGAGCAG GCAGGTCCCA 
GATGTTGCCC GGGAGCTGAG CGGCGGCTGC CATGGCTTCT CACGCAAGTT TATGCATTGG 
CAGGAGGAGC TGCTGCTGGG GAGCCCGGTG AGGAGCCCTC AGGGCCGGCT GCTCAGTGCG 
GAAGCCCTGC AGAGCACCTT CCTGCTCATG AGCCCCCGGC AGCTGTATGA CCATTACCGA 
GGAGACTATG AAACCCATGA CATCAGCTGG AGCGAGTCCC AGGCAGGCGC TGTGCTGCAG 
GCCTGGCAGA GGCGCTTCGT GGAGCTGGCC CAGCAGGCCA TCCCCCAGAA TGCGTCCCAG 
CAGATCCACG CCTTCTCTGC CACCACCCTG GATGACCTCT TGCGCTCCTT CTCTGACATC 
AGTGCAGTCC GTGTGGCGGG GGGCTACCTC CTCATGCTGG CTTACGCCTG CGTGACCATG 
CTCCGCTGGG ACTGCTCCAA GTCTCAGGGG GCCGTGGGCC TGGCCGGGGT CCTTCTGGTA 
GCCCTGTCTG TGGCCTCTGG CCTCGGGCTC TGCTCGCTGC TGGGTATGAC CTTCAATGCT 
GCTACGACTC AGGTGCTCCC ATTCCTGGCC CTGGGCATCG GGGTGGACGA CATGTTCCTG 
CTGGCCCACG CCTTCACCGA GGCCCCCTCG GGCACCCCCC TGCAGGAGCG GACCGGCGAG 
TGCCTGCAGC GCATGGGCAC CAGCGTGGTG CTCACCTCCG TCAACAACCT GGTCGCCTTC 
TTCATGGCCG CCCTGGTGCC CATCCCCGCC CTTCGCGCCT TCTCCCTCCA GGCTGCGGTG 
GTGGTCGGCT GCAACTTTGC TGCTGTGCTG CTCATCTTCC CGGCCATCCT CAGCTTGGAC 
CTGCACCGGC GGCACCGGCA GCGGCTGGAT GTGCTCTGCT GCTTCTCCAG CCCATGTTCT 
TCCCGGGTGA TTCAGATCCA GCCTCAGGAG CTGGGGGAGG GGCAGGTGCC CAGGAGCGGG 
GCCCACCTGA CAGCCACGGT CCAGGCCTTC GCCCGCTGCG ACACAGCCGG CCAGCACGTC 
GTCACCATCC TGCCCCCCAC CACTCACCTG ACGTCCCTCC CCACCGAGTC TCTGGGCTCC 
CAGCTCTTTG GCCCCATGGG TTCAACCAGA GACTTGCTGG GCCAGGTGCC CGGGCCGGGC 
CGGGGCCAGG TGTGCCGCCC GCTGCCCTGT GCCCGCTGGA ACCTCTCTCG CTTTGCCCGG 
TGCCAGTACG CCCCCCTGCT GTTGCAGCCC CGAACCAAGG GCCTGGTACT GGTGCTTTTC 
GGGGCCCTCC TGGGGCTGAG CCTGTACGGA GCCACGTGGG TGCAGGATGG GCTGACCCTG 
ACGGATGTGG TGCCCCGGGG CACCAAGGAG TACGACTTCC TGGCCGCCCA GATCAAGTAC 
TTCTCCCTGT ACGAGGTGGC CCTTGTGACC CAGGGTGGCT TCGACTACGC CCGCTCCCAG 
CAGGCCCTGC TGGACCTGCA CAAACGCTTC AGCGCCCTCA AATCCGTGCT GGCGCCGCAG 
CCACCGAGAA GCTGGCTACA CCGCTACAGC GCCTGGCTCC AGGGGCTCCA GGCCGCCTTT 
GACCAAGACT GGGCCGCCGG CCGCATCACG CGCCACTCGT GCCGCAACGG CTCCGAGGAT 
GGAGCCCTGG CCTACAGGCT GCTCATCCAG ACGGGGGACG CCAAGGAGCC GCTGGACTAC 
AGCCAGCTGG ACAAGCGGAA ACTGGTGGAT GCTGATGGCC TCATCCTGCC CGAGCTCTTC 
TACGTTGGGC TGACCGTGTG GGTGAGCAGA GACCCTTTGG GCCTGGCAGC CTCCCAGGCC 
AACTTCTACC CCCCGCCCCC CGAGTGGCTC CACGACAAAT ATGACAGCAC TGGGGAGAGC 
TTGCACATCC CTGCGGCCCA GCCTTTGGAG TTTGCCCAGT TCCCCTTCCT GCTGAGCGGG 
CTCCGCCAGA CGGCAGACTT CGTGGAGGCC ATCGAGGGGG CCCGGGCGGC CTGCGCAGAG 
GCGGCCCGCG CTGGGATCCG GGCCTACCCC AGCGGCTCCC CGTTCCTCTT CTGGGAGCAG 
TACTTGGGCC TCCGCCGCTG CTTTCTGCTG GCCGTCTGCG TGCTGCTGGC CTGCACCTTC 
GTGGTCTGTG CCTTGCTGCT GCTCAGCCCC TGGACCGCCG GCCTCATTGT GCTGGTGCTG 
GCCATGATGA CTGTGGAATT ATTCGGCATC ATGGGCTTCC TGGGCATCAA ACTCAGCGCC 
ATCCCCGTGG TGATTCTCGT GGCCTCCGTG GGCATTGGCG TCGAGTTCAC AGCTCACGTG 
GCTCTGGGCT TCCTGACAGC CACCGGCAGC CGGGACGCGC GGTCAGCCCA GGCCTTGGAG 
CACATGTTTG CGCCCGTGAT GGATGGGGCC GTGTCCACGC TGCTGGGCCT GCTCATGCTG 
GCCGGCTCGG ACTTCGACTT TATTGTCAGG TACTTCTTTG TGGTGCTGAC AGTGCTGACC 
GGCCTGGGCC TGCTTCACGG GCTTGTTCTG CTTCCTGTGC TGCTGTCCAT CATAGGACCC 
CCACCCCAGG TACCGCTGCC AGATGGAGGC GGCCGCCCGC CCTGCCCTGA CCCGGTATCG 
CTGCCCCTCA GTCCTCCTCA CTTCTTCCTG GGCCCGAGCC CAGCCTTCAG GGCTCCTGAG 
ACAGGAGCAG GGGACGCCCC AAGCACCTTC ATCCTGCCCC CGGCCCACTC CCACATCTTG 
GTGGAGGCCA GCAAGGACCC CAGCTTCCCG ACCATCACGG TGGTGCAGAC GTACAAGGAT 
GGTTCTCCTG GCTCCGGGCT ACCTCCCCTT ACGGCCACGG CCGGCAGTGA GGCCCGCTGG 
GGGCCCCATG CCAGCCCCGG CGCCTTCACC ACCGTGACGG CCTCCGTCAC CGTGGCCCTG 
CACCCGCCGC CACTGCCGGG TAGCTACGTC CAGGAGGTCC CTGAGGAAGC CAGGCCCCCG 
ATGGCCGGCG AGTCCAAGGG ACCTGGCCCC CGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_012547495.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012402949.1 Sarcophilus harrisii patched 2 (PTCH2), mRNA.

Price & Availability↑

CloneID	OSa129083
Clone ID Related Accession (Same CDS sequence)	XM_012547495.1 , XM_012547495.2
Accession Version	XM_012547495.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein patched homolog 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 26% of CDS bases ##RefSeq-Attributes-END##

ATGGGGGAGG GATTGGAGCC CACCCCCCAA AATGTGCCTC CGTTCTCCCC ACCGGGCTCC 
AGATGGGGCA GTGGCTCAGC TGGGGCTGCA GCTGCTTCCC TCAGAGCCCT GGCTCGGTTA 
TTGATAACAG GAGAGATGCG GAGCCCTGCT GGTCCTGCCT CCTCCCGGAC TGACCAGCGC 
TTATCGGCTG CCTTTGGCTG GCACTCGGGG CTAGCCTCTG CGGAGCAGGC TCCCTGGGAA 
CTGAACCCTG TAGTCTTCCA GAGCCTGAGG GAGGGGGTTG GGGGAGTAGT GGGTCAGAAG 
TCAGGCCGCA GGGAGGTGAG GAGCGCATGC ATCCCACAGA ACACGGACTT TGCAGCTTCC 
CTGCACACGT TGCCCAGAAC GCTGAAGACA CTCGTGGATC AGCTGACTTT GTGCCTGGTG 
CTGGAGATGG AGACAAAAGA CAAAGGCCGG GCCCCCCTCT GGTTGCGAGC CCACGTGGGC 
GGCTTGCTCT TCAGCTTGGG CTGCGCGATC CAGCGGCATT GTGGCAAGGT GCTCTTCGTG 
GGGCTGCTGG CTTTCGGGGC CCTTGCGCTG GGCCTGCGTG GGGCTGCCGT GGAGACAGAC 
CTGGAGCGCC TCTGGGTGGA AGTGGGCAGC CGGGTGAGCC AGGAGCTCCG TTACACAAAG 
GAGAAGCTCG GGGAAGAGGC CGTGTACACC TCTCAGACCC TCATCCAGAC CGCCCGTCAT 
CCGGGCGAGA GCGTTCTCAC ACCCGAGGCT CTGGGCCTCC ACCTTCAGGC TGCCTTGGCA 
GCTAGCAAAG TTCAAGTTTC CCTCTATGGA AAGTCCTGGG ATCTGAACAA AATCTGCTAC 
AAGGCAGGAG TCCCTGTTAT TGAGAATGGG ATGATTGAGC GGATGATCGA GAAGCTGTTT 
CCCTGTGTGA TCCTCACCCC GCTGGACTGC TTTTGGGAGG GAGCCAAACT TCAGGGGGGC 
TCCGCGTACC TACCGGGCCG ACCTGATATT CAGTGGACCA ACCTGGATCC TGAGCAACTT 
CTGGAAGAAC TCGGCCCCTT TGCCTCCCTG GAGGGCTTTA GGGAGCTGCT GGACAAGGCC 
CAGGTGGGCC AGGCCTACGT GGGACGGCCC TGCCTGCATC CCAGTGATCC CCATTGCCCA 
GCCAGTGCCC CTAATCGCCA GAGCAGGCAG GTCCCAGATG TTGCCCGGGA GCTGAGCGGC 
GGCTGCCATG GCTTCTCACG CAAGTTTATG CATTGGCAGG AGGAGCTGCT GCTGGGGAGC 
CCGGTGAGGA GCCCTCAGGG CCGGCTGCTC AGTGCGGAAG CCCTGCAGAG CACCTTCCTG 
CTCATGAGCC CCCGGCAGCT GTATGACCAT TACCGAGGAG ACTATGAAAC CAATGACATC 
AGCTGGAGCG AGTCCCAGGC AGGCGCTGTG CTGCAGGCCT GGCAGAGGCG CTTCGTGGAG 
CTGGCCCAGC AGGCCATCCC CCAGAATGCG TCCCAGCAGA TCCACGCCTT CTCTGCCACC 
ACCCTGGATG ACCTCTTGCG CTCCTTCTCT GACATCAGTG CAGTCCGTGT GGCGGGGGGC 
TACCTCCTCA TGCTGGCTTA CGCCTGCGTG ACCATGCTCC GCTGGGACTG CTCCAAGTCT 
CAGGGGGCCG TGGGCCTGGC CGGGGTCCTT CTGGTAGCCC TGTCTGTGGC CTCTGGCCTC 
GGGCTCTGCT CGCTGCTGGG TATGACCTTC AATGCTGCTA CGACTCAGGT GCTCCCATTC 
CTGGCCCTGG GCATCGGGGT GGACGACATG TTCCTGCTGG CCCACGCCTT CACCGAGGCC 
CCCTCGGGCA CCCCCCTGCA GGAGCGGACC GGCGAGTGCC TGCAGCGCAT GGGCACCAGC 
GTGGTGCTCA CCTCCGTCAA CAACCTGGTC GCCTTCTTCA TGGCCGCCCT GGTGCCCATC 
CCCGCCCTTC GCGCCTTCTC CCTCCAGGCT GCGGTGGTGG TCGGCTGCAA CTTTGCTGCT 
GTGCTGCTCA TCTTCCCGGC CATCCTCAGC TTGGACCTGC ACCGGCGGCA CCGGCAGCGG 
CTGGATGTGC TCTGCTGCTT CTCCAGCCCA TGTTCTTCCC GGGTGATTCA GATCCAGCCT 
CAGGAGCTGG GGGAGGGGCA GGTGCCCAGG AGCGGGGCCC ACCTGACAGC CACGGTCCAG 
GCCTTCGCCC GCTGCGACAC AGCCGGCCAG CACGTCGTCA CCATCCTGCC CCCCACCACT 
CACCTGACGT CCCTCCCCAC CGAGTCTCTG GGCTCCCAGC TCTTTGGCCC CATGGGTTCA 
ACCAGAGACT TGCTGGGCCA GGTGCCCGGG CCGGGCCGGG GCCAGGTGTG CCGCCCGCTG 
CCCTGTGCCC GCTGGAACCT CTCTCGCTTT GCCCGGTGCC AGTACGCCCC CCTGCTGTTG 
CAGCCCCGAA CCAAGGGCCT GGTACTGGTG CTTTTCGGGG CCCTCCTGGG GCTGAGCCTG 
TACGGAAGCT GCACCAGAGA GTGGGGCCTG GCCAGAGCCT CCTCTCCGGA GGAGCAGCAG 
AGGGGGACGC GCTGCGCCCG AGCGGGATCG CCCGGCCCTG GGACCCTCGG CCTTGGAGGA 
GGCGGGCAGC CAAGGCTTGA AATCCGGGCC GTTGGCAGAG AGCAGAAGAG CAGCCCCGTC 
TGTGCCTTCC TTTATTGGGA TGTAGCCTCA CTTGGGCCTG GAGACACCGC CGTGCCCCAC 
CCCCCCCAGG CCTTTCAGGC CCGGCCCTTT CTCCACCCTC CAGAAGTGGG AGCAGGGGAA 
GAAGGGAGCC GGACAACCGA GGACCCCTTG AGGCCTTGCT GTGGGCCTGG GGGCAGAGAA 
GCCAGCTGCC GGTTCCGATC TGCTCTCCCG TGTGTGCTGG AGCGGGAAGG AGCGGCTCCA 
TCTGCTTCCC AGCCCAGGGC AATGGCCAAC CCTCGAGCTC GTGTTGCAGG GGCCATAGGG 
GGCCGGGCCC CGTGCTCGGC CCCCACAGCG TCCCTGCTTT CGGGACCCTC CCGTTCTAAC 
CACGGGACCT CTGTTAGTGG CTGCCAGGAT TGCATGCAGC GAGCTGTCTC TCTCTTCTCT 
CTCAATGACC CGGCCCTGAG CCCCGGCCTT GCACCAGCAC ACAGCCCGGT CCACTCGAGC 
CTGGAGAGGA CGCCCACTAC CCCCCGCACG ACCCCCACCA TGAGCTGGGG CCTGGGTCCA 
CAGAGCGAGG TCGGGGCCTG CCATCAGCCC TCTTCTCCCC CCCCACCCAA GCTGGACAAG 
CGGAAACTGG TGGATGCTGA TGGCCTCATC CTGCCCGAGC TCTTCTACGT TGGGCTGACC 
GTGTGGGTGA GCAGAGACCC TTTGGGCCTG GCAGCCTCCC AGGCCAACTT CTACCCCCCG 
CCCCCCGAGT GGCTCCACGA CAAATATGAC AGCACTGGGG AGAGCTTGCA CATCCCTGCG 
GCCCAGCCTT TGGAGTTTGC CCAGTTCCCC TTCCTGCTGA GCGGGCTCCG CCAGACGGCA 
GACTTCGTGG AGGCCATCGA GGGGGCCCGG GCGGCCTGCG CAGAGGCGGC CCGCGCTGGG 
ATCCGGGCCT ACCCCAGCGG CTCCCCGTTC CTCTTCTGGG AGCAGTACTT GGGCCTCCGC 
CGCTGCTTTC TGCTGGCCGT CTGCGTGCTG CTGGCCTGCA CCTTCGTGGT CTGTGCCTTG 
CTGCTGCTCA GCCCCTGGAC CGCCGGCCTC ATTGTGCTGG TGCTGGCCAT GATGACTGTG 
GAATTATTCG GCATCATGGG CTTCCTGGGC ATCAAACTCA GCGCCATCCC CGTGGTGATT 
CTCGTGGCCT CCGTGGGCAT TGGCGTCGAG TTCACAGCTC ACGTGGCTCT GGGCTTCCTG 
ACAGCCACCG GCAGCCGGGA CGCGCGGTCA GCCCAGGCCT TGGAGCACAT GTTTGCGCCC 
GTGATGGATG GGGCCGTGTC CACGCTGCTG GGCCTGCTCA TGCTGGCCGG CTCGGACTTC 
GACTTTATTG TCAGGTACTT CTTTGTGGTG CTGACAGTGC TGACCGGCCT GGGCCTGCTT 
CACGGGCTTG TTCTGCTTCC TGTGCTGCTG TCCATCATAG GACCCCCACC CCAGGTACCG 
CTGCCAGATG GAGGCGGCCG CCCGCCCTGC CCTGACCCGG TATCGCTGCC CCTCAGTCCT 
CCTCACTTCT TCCTGGGCCC GAGCCCAGCC TTCAGGGCTC CTGAGACAGG AGCAGGGGAC 
GCCCCAAGCA CCTTCATCCT GCCCCCGGCC CACTCCCACA TCTTGGTGGA GGCCAGCAAG 
GACCCCAGCT TCCCGACCAT CACGGTGGTG CAGACGTACA AGGATGGTTC TCCTGGCTCC 
GGGCTACCTC CCCTTACGGC CACGGCCGGC AGTGAGGCCC GCTGGGGGCC CCATGCCAGC 
CCCGGCGCCT TCACCACCGT GACGGCCTCC GTCACCGTGG CCCTGCACCC GCCGCCACTG 
CCGGGTAGCT ACGTCCAGGA GGTCCCTGAG GAAGCCAGGC CCCCGATGGC CGGCGAGTCC 
AAGGGACCTG GCCCCCGCTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012402949.1
CDS	1..4524
Translation	MGEGLEPTPQNVPPFSPPGSRWGSGSAGAAAASLRALARLLITGEMRSPAGPASSRTDQRLSAAFGWHSGLASAEQAPWELNPVVFQSLREGVGGVVGQKSGRREVRSACIPQNTDFAASLHTLPRTLKTLVDQLTLCLVLEMETKDKGRAPLWLRAHVGGLLFSLGCAIQRHCGKVLFVGLLAFGALALGLRGAAVETDLERLWVEVGSRVSQELRYTKEKLGEEAVYTSQTLIQTARHPGESVLTPEALGLHLQAALAASKVQVSLYGKSWDLNKICYKAGVPVIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPSDPHCPASAPNRQSRQVPDVARELSGGCHGFSRKFMHWQEELLLGSPVRSPQGRLLSAEALQSTFLLMSPRQLYDHYRGDYETNDISWSESQAGAVLQAWQRRFVELAQQAIPQNASQQIHAFSATTLDDLLRSFSDISAVRVAGGYLLMLAYACVTMLRWDCSKSQGAVGLAGVLLVALSVASGLGLCSLLGMTFNAATTQVLPFLALGIGVDDMFLLAHAFTEAPSGTPLQERTGECLQRMGTSVVLTSVNNLVAFFMAALVPIPALRAFSLQAAVVVGCNFAAVLLIFPAILSLDLHRRHRQRLDVLCCFSSPCSSRVIQIQPQELGEGQVPRSGAHLTATVQAFARCDTAGQHVVTILPPTTHLTSLPTESLGSQLFGPMGSTRDLLGQVPGPGRGQVCRPLPCARWNLSRFARCQYAPLLLQPRTKGLVLVLFGALLGLSLYGSCTREWGLARASSPEEQQRGTRCARAGSPGPGTLGLGGGGQPRLEIRAVGREQKSSPVCAFLYWDVASLGPGDTAVPHPPQAFQARPFLHPPEVGAGEEGSRTTEDPLRPCCGPGGREASCRFRSALPCVLEREGAAPSASQPRAMANPRARVAGAIGGRAPCSAPTASLLSGPSRSNHGTSVSGCQDCMQRAVSLFSLNDPALSPGLAPAHSPVHSSLERTPTTPRTTPTMSWGLGPQSEVGACHQPSSPPPPKLDKRKLVDADGLILPELFYVGLTVWVSRDPLGLAASQANFYPPPPEWLHDKYDSTGESLHIPAAQPLEFAQFPFLLSGLRQTADFVEAIEGARAACAEAARAGIRAYPSGSPFLFWEQYLGLRRCFLLAVCVLLACTFVVCALLLLSPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTAHVALGFLTATGSRDARSAQALEHMFAPVMDGAVSTLLGLLMLAGSDFDFIVRYFFVVLTVLTGLGLLHGLVLLPVLLSIIGPPPQVPLPDGGGRPPCPDPVSLPLSPPHFFLGPSPAFRAPETGAGDAPSTFILPPAHSHILVEASKDPSFPTITVVQTYKDGSPGSGLPPLTATAGSEARWGPHASPGAFTTVTASVTVALHPPPLPGSYVQEVPEEARPPMAGESKGPGPRC

Target ORF information:

RefSeq Version	XM_012547495.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii patched 2 (PTCH2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012547495.1

ORF Insert Sequence:

ATGGGGGAGG GATTGGAGCC CACCCCCCAA AATGTGCCTC CGTTCTCCCC ACCGGGCTCC 
AGATGGGGCA GTGGCTCAGC TGGGGCTGCA GCTGCTTCCC TCAGAGCCCT GGCTCGGTTA 
TTGATAACAG GAGAGATGCG GAGCCCTGCT GGTCCTGCCT CCTCCCGGAC TGACCAGCGC 
TTATCGGCTG CCTTTGGCTG GCACTCGGGG CTAGCCTCTG CGGAGCAGGC TCCCTGGGAA 
CTGAACCCTG TAGTCTTCCA GAGCCTGAGG GAGGGGGTTG GGGGAGTAGT GGGTCAGAAG 
TCAGGCCGCA GGGAGGTGAG GAGCGCATGC ATCCCACAGA ACACGGACTT TGCAGCTTCC 
CTGCACACGT TGCCCAGAAC GCTGAAGACA CTCGTGGATC AGCTGACTTT GTGCCTGGTG 
CTGGAGATGG AGACAAAAGA CAAAGGCCGG GCCCCCCTCT GGTTGCGAGC CCACGTGGGC 
GGCTTGCTCT TCAGCTTGGG CTGCGCGATC CAGCGGCATT GTGGCAAGGT GCTCTTCGTG 
GGGCTGCTGG CTTTCGGGGC CCTTGCGCTG GGCCTGCGTG GGGCTGCCGT GGAGACAGAC 
CTGGAGCGCC TCTGGGTGGA AGTGGGCAGC CGGGTGAGCC AGGAGCTCCG TTACACAAAG 
GAGAAGCTCG GGGAAGAGGC CGTGTACACC TCTCAGACCC TCATCCAGAC CGCCCGTCAT 
CCGGGCGAGA GCGTTCTCAC ACCCGAGGCT CTGGGCCTCC ACCTTCAGGC TGCCTTGGCA 
GCTAGCAAAG TTCAAGTTTC CCTCTATGGA AAGTCCTGGG ATCTGAACAA AATCTGCTAC 
AAGGCAGGAG TCCCTGTTAT TGAGAATGGG ATGATTGAGC GGATGATCGA GAAGCTGTTT 
CCCTGTGTGA TCCTCACCCC GCTGGACTGC TTTTGGGAGG GAGCCAAACT TCAGGGGGGC 
TCCGCGTACC TACCGGGCCG ACCTGATATT CAGTGGACCA ACCTGGATCC TGAGCAACTT 
CTGGAAGAAC TCGGCCCCTT TGCCTCCCTG GAGGGCTTTA GGGAGCTGCT GGACAAGGCC 
CAGGTGGGCC AGGCCTACGT GGGACGGCCC TGCCTGCATC CCAGTGATCC CCATTGCCCA 
GCCAGTGCCC CTAATCGCCA GAGCAGGCAG GTCCCAGATG TTGCCCGGGA GCTGAGCGGC 
GGCTGCCATG GCTTCTCACG CAAGTTTATG CATTGGCAGG AGGAGCTGCT GCTGGGGAGC 
CCGGTGAGGA GCCCTCAGGG CCGGCTGCTC AGTGCGGAAG CCCTGCAGAG CACCTTCCTG 
CTCATGAGCC CCCGGCAGCT GTATGACCAT TACCGAGGAG ACTATGAAAC CAATGACATC 
AGCTGGAGCG AGTCCCAGGC AGGCGCTGTG CTGCAGGCCT GGCAGAGGCG CTTCGTGGAG 
CTGGCCCAGC AGGCCATCCC CCAGAATGCG TCCCAGCAGA TCCACGCCTT CTCTGCCACC 
ACCCTGGATG ACCTCTTGCG CTCCTTCTCT GACATCAGTG CAGTCCGTGT GGCGGGGGGC 
TACCTCCTCA TGCTGGCTTA CGCCTGCGTG ACCATGCTCC GCTGGGACTG CTCCAAGTCT 
CAGGGGGCCG TGGGCCTGGC CGGGGTCCTT CTGGTAGCCC TGTCTGTGGC CTCTGGCCTC 
GGGCTCTGCT CGCTGCTGGG TATGACCTTC AATGCTGCTA CGACTCAGGT GCTCCCATTC 
CTGGCCCTGG GCATCGGGGT GGACGACATG TTCCTGCTGG CCCACGCCTT CACCGAGGCC 
CCCTCGGGCA CCCCCCTGCA GGAGCGGACC GGCGAGTGCC TGCAGCGCAT GGGCACCAGC 
GTGGTGCTCA CCTCCGTCAA CAACCTGGTC GCCTTCTTCA TGGCCGCCCT GGTGCCCATC 
CCCGCCCTTC GCGCCTTCTC CCTCCAGGCT GCGGTGGTGG TCGGCTGCAA CTTTGCTGCT 
GTGCTGCTCA TCTTCCCGGC CATCCTCAGC TTGGACCTGC ACCGGCGGCA CCGGCAGCGG 
CTGGATGTGC TCTGCTGCTT CTCCAGCCCA TGTTCTTCCC GGGTGATTCA GATCCAGCCT 
CAGGAGCTGG GGGAGGGGCA GGTGCCCAGG AGCGGGGCCC ACCTGACAGC CACGGTCCAG 
GCCTTCGCCC GCTGCGACAC AGCCGGCCAG CACGTCGTCA CCATCCTGCC CCCCACCACT 
CACCTGACGT CCCTCCCCAC CGAGTCTCTG GGCTCCCAGC TCTTTGGCCC CATGGGTTCA 
ACCAGAGACT TGCTGGGCCA GGTGCCCGGG CCGGGCCGGG GCCAGGTGTG CCGCCCGCTG 
CCCTGTGCCC GCTGGAACCT CTCTCGCTTT GCCCGGTGCC AGTACGCCCC CCTGCTGTTG 
CAGCCCCGAA CCAAGGGCCT GGTACTGGTG CTTTTCGGGG CCCTCCTGGG GCTGAGCCTG 
TACGGAAGCT GCACCAGAGA GTGGGGCCTG GCCAGAGCCT CCTCTCCGGA GGAGCAGCAG 
AGGGGGACGC GCTGCGCCCG AGCGGGATCG CCCGGCCCTG GGACCCTCGG CCTTGGAGGA 
GGCGGGCAGC CAAGGCTTGA AATCCGGGCC GTTGGCAGAG AGCAGAAGAG CAGCCCCGTC 
TGTGCCTTCC TTTATTGGGA TGTAGCCTCA CTTGGGCCTG GAGACACCGC CGTGCCCCAC 
CCCCCCCAGG CCTTTCAGGC CCGGCCCTTT CTCCACCCTC CAGAAGTGGG AGCAGGGGAA 
GAAGGGAGCC GGACAACCGA GGACCCCTTG AGGCCTTGCT GTGGGCCTGG GGGCAGAGAA 
GCCAGCTGCC GGTTCCGATC TGCTCTCCCG TGTGTGCTGG AGCGGGAAGG AGCGGCTCCA 
TCTGCTTCCC AGCCCAGGGC AATGGCCAAC CCTCGAGCTC GTGTTGCAGG GGCCATAGGG 
GGCCGGGCCC CGTGCTCGGC CCCCACAGCG TCCCTGCTTT CGGGACCCTC CCGTTCTAAC 
CACGGGACCT CTGTTAGTGG CTGCCAGGAT TGCATGCAGC GAGCTGTCTC TCTCTTCTCT 
CTCAATGACC CGGCCCTGAG CCCCGGCCTT GCACCAGCAC ACAGCCCGGT CCACTCGAGC 
CTGGAGAGGA CGCCCACTAC CCCCCGCACG ACCCCCACCA TGAGCTGGGG CCTGGGTCCA 
CAGAGCGAGG TCGGGGCCTG CCATCAGCCC TCTTCTCCCC CCCCACCCAA GCTGGACAAG 
CGGAAACTGG TGGATGCTGA TGGCCTCATC CTGCCCGAGC TCTTCTACGT TGGGCTGACC 
GTGTGGGTGA GCAGAGACCC TTTGGGCCTG GCAGCCTCCC AGGCCAACTT CTACCCCCCG 
CCCCCCGAGT GGCTCCACGA CAAATATGAC AGCACTGGGG AGAGCTTGCA CATCCCTGCG 
GCCCAGCCTT TGGAGTTTGC CCAGTTCCCC TTCCTGCTGA GCGGGCTCCG CCAGACGGCA 
GACTTCGTGG AGGCCATCGA GGGGGCCCGG GCGGCCTGCG CAGAGGCGGC CCGCGCTGGG 
ATCCGGGCCT ACCCCAGCGG CTCCCCGTTC CTCTTCTGGG AGCAGTACTT GGGCCTCCGC 
CGCTGCTTTC TGCTGGCCGT CTGCGTGCTG CTGGCCTGCA CCTTCGTGGT CTGTGCCTTG 
CTGCTGCTCA GCCCCTGGAC CGCCGGCCTC ATTGTGCTGG TGCTGGCCAT GATGACTGTG 
GAATTATTCG GCATCATGGG CTTCCTGGGC ATCAAACTCA GCGCCATCCC CGTGGTGATT 
CTCGTGGCCT CCGTGGGCAT TGGCGTCGAG TTCACAGCTC ACGTGGCTCT GGGCTTCCTG 
ACAGCCACCG GCAGCCGGGA CGCGCGGTCA GCCCAGGCCT TGGAGCACAT GTTTGCGCCC 
GTGATGGATG GGGCCGTGTC CACGCTGCTG GGCCTGCTCA TGCTGGCCGG CTCGGACTTC 
GACTTTATTG TCAGGTACTT CTTTGTGGTG CTGACAGTGC TGACCGGCCT GGGCCTGCTT 
CACGGGCTTG TTCTGCTTCC TGTGCTGCTG TCCATCATAG GACCCCCACC CCAGGTACCG 
CTGCCAGATG GAGGCGGCCG CCCGCCCTGC CCTGACCCGG TATCGCTGCC CCTCAGTCCT 
CCTCACTTCT TCCTGGGCCC GAGCCCAGCC TTCAGGGCTC CTGAGACAGG AGCAGGGGAC 
GCCCCAAGCA CCTTCATCCT GCCCCCGGCC CACTCCCACA TCTTGGTGGA GGCCAGCAAG 
GACCCCAGCT TCCCGACCAT CACGGTGGTG CAGACGTACA AGGATGGTTC TCCTGGCTCC 
GGGCTACCTC CCCTTACGGC CACGGCCGGC AGTGAGGCCC GCTGGGGGCC CCATGCCAGC 
CCCGGCGCCT TCACCACCGT GACGGCCTCC GTCACCGTGG CCCTGCACCC GCCGCCACTG 
CCGGGTAGCT ACGTCCAGGA GGTCCCTGAG GAAGCCAGGC CCCCGATGGC CGGCGAGTCC 
AAGGGACCTG GCCCCCGCTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTCH2 ( XM_012547495.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012402949.1 Sarcophilus harrisii patched 2 (PTCH2), mRNA.

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CloneID	OSa129083
Clone ID Related Accession (Same CDS sequence)	XM_012547495.1 , XM_012547495.2
Accession Version	XM_012547495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	protein patched homolog 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003838737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 11, 2018 this sequence version replaced XM_012547495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 26% of CDS bases ##RefSeq-Attributes-END##

ATGGGGGAGG GATTGGAGCC CACCCCCCAA AATGTGCCTC CGTTCTCCCC ACCGGGCTCC 
AGATGGGGCA GTGGCTCAGC TGGGGCTGCA GCTGCTTCCC TCAGAGCCCT GGCTCGGTTA 
TTGATAACAG GAGAGATGCG GAGCCCTGCT GGTCCTGCCT CCTCCCGGAC TGACCAGCGC 
TTATCGGCTG CCTTTGGCTG GCACTCGGGG CTAGCCTCTG CGGAGCAGGC TCCCTGGGAA 
CTGAACCCTG TAGTCTTCCA GAGCCTGAGG GAGGGGGTTG GGGGAGTAGT GGGTCAGAAG 
TCAGGCCGCA GGGAGGTGAG GAGCGCATGC ATCCCACAGA ACACGGACTT TGCAGCTTCC 
CTGCACACGT TGCCCAGAAC GCTGAAGACA CTCGTGGATC AGCTGACTTT GTGCCTGGTG 
CTGGAGATGG AGACAAAAGA CAAAGGCCGG GCCCCCCTCT GGTTGCGAGC CCACGTGGGC 
GGCTTGCTCT TCAGCTTGGG CTGCGCGATC CAGCGGCATT GTGGCAAGGT GCTCTTCGTG 
GGGCTGCTGG CTTTCGGGGC CCTTGCGCTG GGCCTGCGTG GGGCTGCCGT GGAGACAGAC 
CTGGAGCGCC TCTGGGTGGA AGTGGGCAGC CGGGTGAGCC AGGAGCTCCG TTACACAAAG 
GAGAAGCTCG GGGAAGAGGC CGTGTACACC TCTCAGACCC TCATCCAGAC CGCCCGTCAT 
CCGGGCGAGA GCGTTCTCAC ACCCGAGGCT CTGGGCCTCC ACCTTCAGGC TGCCTTGGCA 
GCTAGCAAAG TTCAAGTTTC CCTCTATGGA AAGTCCTGGG ATCTGAACAA AATCTGCTAC 
AAGGCAGGAG TCCCTGTTAT TGAGAATGGG ATGATTGAGC GGATGATCGA GAAGCTGTTT 
CCCTGTGTGA TCCTCACCCC GCTGGACTGC TTTTGGGAGG GAGCCAAACT TCAGGGGGGC 
TCCGCGTACC TACCGGGCCG ACCTGATATT CAGTGGACCA ACCTGGATCC TGAGCAACTT 
CTGGAAGAAC TCGGCCCCTT TGCCTCCCTG GAGGGCTTTA GGGAGCTGCT GGACAAGGCC 
CAGGTGGGCC AGGCCTACGT GGGACGGCCC TGCCTGCATC CCAGTGATCC CCATTGCCCA 
GCCAGTGCCC CTAATCGCCA GAGCAGGCAG GTCCCAGATG TTGCCCGGGA GCTGAGCGGC 
GGCTGCCATG GCTTCTCACG CAAGTTTATG CATTGGCAGG AGGAGCTGCT GCTGGGGAGC 
CCGGTGAGGA GCCCTCAGGG CCGGCTGCTC AGTGCGGAAG CCCTGCAGAG CACCTTCCTG 
CTCATGAGCC CCCGGCAGCT GTATGACCAT TACCGAGGAG ACTATGAAAC CAATGACATC 
AGCTGGAGCG AGTCCCAGGC AGGCGCTGTG CTGCAGGCCT GGCAGAGGCG CTTCGTGGAG 
CTGGCCCAGC AGGCCATCCC CCAGAATGCG TCCCAGCAGA TCCACGCCTT CTCTGCCACC 
ACCCTGGATG ACCTCTTGCG CTCCTTCTCT GACATCAGTG CAGTCCGTGT GGCGGGGGGC 
TACCTCCTCA TGCTGGCTTA CGCCTGCGTG ACCATGCTCC GCTGGGACTG CTCCAAGTCT 
CAGGGGGCCG TGGGCCTGGC CGGGGTCCTT CTGGTAGCCC TGTCTGTGGC CTCTGGCCTC 
GGGCTCTGCT CGCTGCTGGG TATGACCTTC AATGCTGCTA CGACTCAGGT GCTCCCATTC 
CTGGCCCTGG GCATCGGGGT GGACGACATG TTCCTGCTGG CCCACGCCTT CACCGAGGCC 
CCCTCGGGCA CCCCCCTGCA GGAGCGGACC GGCGAGTGCC TGCAGCGCAT GGGCACCAGC 
GTGGTGCTCA CCTCCGTCAA CAACCTGGTC GCCTTCTTCA TGGCCGCCCT GGTGCCCATC 
CCCGCCCTTC GCGCCTTCTC CCTCCAGGCT GCGGTGGTGG TCGGCTGCAA CTTTGCTGCT 
GTGCTGCTCA TCTTCCCGGC CATCCTCAGC TTGGACCTGC ACCGGCGGCA CCGGCAGCGG 
CTGGATGTGC TCTGCTGCTT CTCCAGCCCA TGTTCTTCCC GGGTGATTCA GATCCAGCCT 
CAGGAGCTGG GGGAGGGGCA GGTGCCCAGG AGCGGGGCCC ACCTGACAGC CACGGTCCAG 
GCCTTCGCCC GCTGCGACAC AGCCGGCCAG CACGTCGTCA CCATCCTGCC CCCCACCACT 
CACCTGACGT CCCTCCCCAC CGAGTCTCTG GGCTCCCAGC TCTTTGGCCC CATGGGTTCA 
ACCAGAGACT TGCTGGGCCA GGTGCCCGGG CCGGGCCGGG GCCAGGTGTG CCGCCCGCTG 
CCCTGTGCCC GCTGGAACCT CTCTCGCTTT GCCCGGTGCC AGTACGCCCC CCTGCTGTTG 
CAGCCCCGAA CCAAGGGCCT GGTACTGGTG CTTTTCGGGG CCCTCCTGGG GCTGAGCCTG 
TACGGAAGCT GCACCAGAGA GTGGGGCCTG GCCAGAGCCT CCTCTCCGGA GGAGCAGCAG 
AGGGGGACGC GCTGCGCCCG AGCGGGATCG CCCGGCCCTG GGACCCTCGG CCTTGGAGGA 
GGCGGGCAGC CAAGGCTTGA AATCCGGGCC GTTGGCAGAG AGCAGAAGAG CAGCCCCGTC 
TGTGCCTTCC TTTATTGGGA TGTAGCCTCA CTTGGGCCTG GAGACACCGC CGTGCCCCAC 
CCCCCCCAGG CCTTTCAGGC CCGGCCCTTT CTCCACCCTC CAGAAGTGGG AGCAGGGGAA 
GAAGGGAGCC GGACAACCGA GGACCCCTTG AGGCCTTGCT GTGGGCCTGG GGGCAGAGAA 
GCCAGCTGCC GGTTCCGATC TGCTCTCCCG TGTGTGCTGG AGCGGGAAGG AGCGGCTCCA 
TCTGCTTCCC AGCCCAGGGC AATGGCCAAC CCTCGAGCTC GTGTTGCAGG GGCCATAGGG 
GGCCGGGCCC CGTGCTCGGC CCCCACAGCG TCCCTGCTTT CGGGACCCTC CCGTTCTAAC 
CACGGGACCT CTGTTAGTGG CTGCCAGGAT TGCATGCAGC GAGCTGTCTC TCTCTTCTCT 
CTCAATGACC CGGCCCTGAG CCCCGGCCTT GCACCAGCAC ACAGCCCGGT CCACTCGAGC 
CTGGAGAGGA CGCCCACTAC CCCCCGCACG ACCCCCACCA TGAGCTGGGG CCTGGGTCCA 
CAGAGCGAGG TCGGGGCCTG CCATCAGCCC TCTTCTCCCC CCCCACCCAA GCTGGACAAG 
CGGAAACTGG TGGATGCTGA TGGCCTCATC CTGCCCGAGC TCTTCTACGT TGGGCTGACC 
GTGTGGGTGA GCAGAGACCC TTTGGGCCTG GCAGCCTCCC AGGCCAACTT CTACCCCCCG 
CCCCCCGAGT GGCTCCACGA CAAATATGAC AGCACTGGGG AGAGCTTGCA CATCCCTGCG 
GCCCAGCCTT TGGAGTTTGC CCAGTTCCCC TTCCTGCTGA GCGGGCTCCG CCAGACGGCA 
GACTTCGTGG AGGCCATCGA GGGGGCCCGG GCGGCCTGCG CAGAGGCGGC CCGCGCTGGG 
ATCCGGGCCT ACCCCAGCGG CTCCCCGTTC CTCTTCTGGG AGCAGTACTT GGGCCTCCGC 
CGCTGCTTTC TGCTGGCCGT CTGCGTGCTG CTGGCCTGCA CCTTCGTGGT CTGTGCCTTG 
CTGCTGCTCA GCCCCTGGAC CGCCGGCCTC ATTGTGCTGG TGCTGGCCAT GATGACTGTG 
GAATTATTCG GCATCATGGG CTTCCTGGGC ATCAAACTCA GCGCCATCCC CGTGGTGATT 
CTCGTGGCCT CCGTGGGCAT TGGCGTCGAG TTCACAGCTC ACGTGGCTCT GGGCTTCCTG 
ACAGCCACCG GCAGCCGGGA CGCGCGGTCA GCCCAGGCCT TGGAGCACAT GTTTGCGCCC 
GTGATGGATG GGGCCGTGTC CACGCTGCTG GGCCTGCTCA TGCTGGCCGG CTCGGACTTC 
GACTTTATTG TCAGGTACTT CTTTGTGGTG CTGACAGTGC TGACCGGCCT GGGCCTGCTT 
CACGGGCTTG TTCTGCTTCC TGTGCTGCTG TCCATCATAG GACCCCCACC CCAGGTACCG 
CTGCCAGATG GAGGCGGCCG CCCGCCCTGC CCTGACCCGG TATCGCTGCC CCTCAGTCCT 
CCTCACTTCT TCCTGGGCCC GAGCCCAGCC TTCAGGGCTC CTGAGACAGG AGCAGGGGAC 
GCCCCAAGCA CCTTCATCCT GCCCCCGGCC CACTCCCACA TCTTGGTGGA GGCCAGCAAG 
GACCCCAGCT TCCCGACCAT CACGGTGGTG CAGACGTACA AGGATGGTTC TCCTGGCTCC 
GGGCTACCTC CCCTTACGGC CACGGCCGGC AGTGAGGCCC GCTGGGGGCC CCATGCCAGC 
CCCGGCGCCT TCACCACCGT GACGGCCTCC GTCACCGTGG CCCTGCACCC GCCGCCACTG 
CCGGGTAGCT ACGTCCAGGA GGTCCCTGAG GAAGCCAGGC CCCCGATGGC CGGCGAGTCC 
AAGGGACCTG GCCCCCGCTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012402949.1
CDS	1..4524
Translation	MGEGLEPTPQNVPPFSPPGSRWGSGSAGAAAASLRALARLLITGEMRSPAGPASSRTDQRLSAAFGWHSGLASAEQAPWELNPVVFQSLREGVGGVVGQKSGRREVRSACIPQNTDFAASLHTLPRTLKTLVDQLTLCLVLEMETKDKGRAPLWLRAHVGGLLFSLGCAIQRHCGKVLFVGLLAFGALALGLRGAAVETDLERLWVEVGSRVSQELRYTKEKLGEEAVYTSQTLIQTARHPGESVLTPEALGLHLQAALAASKVQVSLYGKSWDLNKICYKAGVPVIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPDIQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPSDPHCPASAPNRQSRQVPDVARELSGGCHGFSRKFMHWQEELLLGSPVRSPQGRLLSAEALQSTFLLMSPRQLYDHYRGDYETNDISWSESQAGAVLQAWQRRFVELAQQAIPQNASQQIHAFSATTLDDLLRSFSDISAVRVAGGYLLMLAYACVTMLRWDCSKSQGAVGLAGVLLVALSVASGLGLCSLLGMTFNAATTQVLPFLALGIGVDDMFLLAHAFTEAPSGTPLQERTGECLQRMGTSVVLTSVNNLVAFFMAALVPIPALRAFSLQAAVVVGCNFAAVLLIFPAILSLDLHRRHRQRLDVLCCFSSPCSSRVIQIQPQELGEGQVPRSGAHLTATVQAFARCDTAGQHVVTILPPTTHLTSLPTESLGSQLFGPMGSTRDLLGQVPGPGRGQVCRPLPCARWNLSRFARCQYAPLLLQPRTKGLVLVLFGALLGLSLYGSCTREWGLARASSPEEQQRGTRCARAGSPGPGTLGLGGGGQPRLEIRAVGREQKSSPVCAFLYWDVASLGPGDTAVPHPPQAFQARPFLHPPEVGAGEEGSRTTEDPLRPCCGPGGREASCRFRSALPCVLEREGAAPSASQPRAMANPRARVAGAIGGRAPCSAPTASLLSGPSRSNHGTSVSGCQDCMQRAVSLFSLNDPALSPGLAPAHSPVHSSLERTPTTPRTTPTMSWGLGPQSEVGACHQPSSPPPPKLDKRKLVDADGLILPELFYVGLTVWVSRDPLGLAASQANFYPPPPEWLHDKYDSTGESLHIPAAQPLEFAQFPFLLSGLRQTADFVEAIEGARAACAEAARAGIRAYPSGSPFLFWEQYLGLRRCFLLAVCVLLACTFVVCALLLLSPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTAHVALGFLTATGSRDARSAQALEHMFAPVMDGAVSTLLGLLMLAGSDFDFIVRYFFVVLTVLTGLGLLHGLVLLPVLLSIIGPPPQVPLPDGGGRPPCPDPVSLPLSPPHFFLGPSPAFRAPETGAGDAPSTFILPPAHSHILVEASKDPSFPTITVVQTYKDGSPGSGLPPLTATAGSEARWGPHASPGAFTTVTASVTVALHPPPLPGSYVQEVPEEARPPMAGESKGPGPRC

Target ORF information:

RefSeq Version	XM_012547495.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii patched 2 (PTCH2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012547495.2

ORF Insert Sequence:

ATGGGGGAGG GATTGGAGCC CACCCCCCAA AATGTGCCTC CGTTCTCCCC ACCGGGCTCC 
AGATGGGGCA GTGGCTCAGC TGGGGCTGCA GCTGCTTCCC TCAGAGCCCT GGCTCGGTTA 
TTGATAACAG GAGAGATGCG GAGCCCTGCT GGTCCTGCCT CCTCCCGGAC TGACCAGCGC 
TTATCGGCTG CCTTTGGCTG GCACTCGGGG CTAGCCTCTG CGGAGCAGGC TCCCTGGGAA 
CTGAACCCTG TAGTCTTCCA GAGCCTGAGG GAGGGGGTTG GGGGAGTAGT GGGTCAGAAG 
TCAGGCCGCA GGGAGGTGAG GAGCGCATGC ATCCCACAGA ACACGGACTT TGCAGCTTCC 
CTGCACACGT TGCCCAGAAC GCTGAAGACA CTCGTGGATC AGCTGACTTT GTGCCTGGTG 
CTGGAGATGG AGACAAAAGA CAAAGGCCGG GCCCCCCTCT GGTTGCGAGC CCACGTGGGC 
GGCTTGCTCT TCAGCTTGGG CTGCGCGATC CAGCGGCATT GTGGCAAGGT GCTCTTCGTG 
GGGCTGCTGG CTTTCGGGGC CCTTGCGCTG GGCCTGCGTG GGGCTGCCGT GGAGACAGAC 
CTGGAGCGCC TCTGGGTGGA AGTGGGCAGC CGGGTGAGCC AGGAGCTCCG TTACACAAAG 
GAGAAGCTCG GGGAAGAGGC CGTGTACACC TCTCAGACCC TCATCCAGAC CGCCCGTCAT 
CCGGGCGAGA GCGTTCTCAC ACCCGAGGCT CTGGGCCTCC ACCTTCAGGC TGCCTTGGCA 
GCTAGCAAAG TTCAAGTTTC CCTCTATGGA AAGTCCTGGG ATCTGAACAA AATCTGCTAC 
AAGGCAGGAG TCCCTGTTAT TGAGAATGGG ATGATTGAGC GGATGATCGA GAAGCTGTTT 
CCCTGTGTGA TCCTCACCCC GCTGGACTGC TTTTGGGAGG GAGCCAAACT TCAGGGGGGC 
TCCGCGTACC TACCGGGCCG ACCTGATATT CAGTGGACCA ACCTGGATCC TGAGCAACTT 
CTGGAAGAAC TCGGCCCCTT TGCCTCCCTG GAGGGCTTTA GGGAGCTGCT GGACAAGGCC 
CAGGTGGGCC AGGCCTACGT GGGACGGCCC TGCCTGCATC CCAGTGATCC CCATTGCCCA 
GCCAGTGCCC CTAATCGCCA GAGCAGGCAG GTCCCAGATG TTGCCCGGGA GCTGAGCGGC 
GGCTGCCATG GCTTCTCACG CAAGTTTATG CATTGGCAGG AGGAGCTGCT GCTGGGGAGC 
CCGGTGAGGA GCCCTCAGGG CCGGCTGCTC AGTGCGGAAG CCCTGCAGAG CACCTTCCTG 
CTCATGAGCC CCCGGCAGCT GTATGACCAT TACCGAGGAG ACTATGAAAC CAATGACATC 
AGCTGGAGCG AGTCCCAGGC AGGCGCTGTG CTGCAGGCCT GGCAGAGGCG CTTCGTGGAG 
CTGGCCCAGC AGGCCATCCC CCAGAATGCG TCCCAGCAGA TCCACGCCTT CTCTGCCACC 
ACCCTGGATG ACCTCTTGCG CTCCTTCTCT GACATCAGTG CAGTCCGTGT GGCGGGGGGC 
TACCTCCTCA TGCTGGCTTA CGCCTGCGTG ACCATGCTCC GCTGGGACTG CTCCAAGTCT 
CAGGGGGCCG TGGGCCTGGC CGGGGTCCTT CTGGTAGCCC TGTCTGTGGC CTCTGGCCTC 
GGGCTCTGCT CGCTGCTGGG TATGACCTTC AATGCTGCTA CGACTCAGGT GCTCCCATTC 
CTGGCCCTGG GCATCGGGGT GGACGACATG TTCCTGCTGG CCCACGCCTT CACCGAGGCC 
CCCTCGGGCA CCCCCCTGCA GGAGCGGACC GGCGAGTGCC TGCAGCGCAT GGGCACCAGC 
GTGGTGCTCA CCTCCGTCAA CAACCTGGTC GCCTTCTTCA TGGCCGCCCT GGTGCCCATC 
CCCGCCCTTC GCGCCTTCTC CCTCCAGGCT GCGGTGGTGG TCGGCTGCAA CTTTGCTGCT 
GTGCTGCTCA TCTTCCCGGC CATCCTCAGC TTGGACCTGC ACCGGCGGCA CCGGCAGCGG 
CTGGATGTGC TCTGCTGCTT CTCCAGCCCA TGTTCTTCCC GGGTGATTCA GATCCAGCCT 
CAGGAGCTGG GGGAGGGGCA GGTGCCCAGG AGCGGGGCCC ACCTGACAGC CACGGTCCAG 
GCCTTCGCCC GCTGCGACAC AGCCGGCCAG CACGTCGTCA CCATCCTGCC CCCCACCACT 
CACCTGACGT CCCTCCCCAC CGAGTCTCTG GGCTCCCAGC TCTTTGGCCC CATGGGTTCA 
ACCAGAGACT TGCTGGGCCA GGTGCCCGGG CCGGGCCGGG GCCAGGTGTG CCGCCCGCTG 
CCCTGTGCCC GCTGGAACCT CTCTCGCTTT GCCCGGTGCC AGTACGCCCC CCTGCTGTTG 
CAGCCCCGAA CCAAGGGCCT GGTACTGGTG CTTTTCGGGG CCCTCCTGGG GCTGAGCCTG 
TACGGAAGCT GCACCAGAGA GTGGGGCCTG GCCAGAGCCT CCTCTCCGGA GGAGCAGCAG 
AGGGGGACGC GCTGCGCCCG AGCGGGATCG CCCGGCCCTG GGACCCTCGG CCTTGGAGGA 
GGCGGGCAGC CAAGGCTTGA AATCCGGGCC GTTGGCAGAG AGCAGAAGAG CAGCCCCGTC 
TGTGCCTTCC TTTATTGGGA TGTAGCCTCA CTTGGGCCTG GAGACACCGC CGTGCCCCAC 
CCCCCCCAGG CCTTTCAGGC CCGGCCCTTT CTCCACCCTC CAGAAGTGGG AGCAGGGGAA 
GAAGGGAGCC GGACAACCGA GGACCCCTTG AGGCCTTGCT GTGGGCCTGG GGGCAGAGAA 
GCCAGCTGCC GGTTCCGATC TGCTCTCCCG TGTGTGCTGG AGCGGGAAGG AGCGGCTCCA 
TCTGCTTCCC AGCCCAGGGC AATGGCCAAC CCTCGAGCTC GTGTTGCAGG GGCCATAGGG 
GGCCGGGCCC CGTGCTCGGC CCCCACAGCG TCCCTGCTTT CGGGACCCTC CCGTTCTAAC 
CACGGGACCT CTGTTAGTGG CTGCCAGGAT TGCATGCAGC GAGCTGTCTC TCTCTTCTCT 
CTCAATGACC CGGCCCTGAG CCCCGGCCTT GCACCAGCAC ACAGCCCGGT CCACTCGAGC 
CTGGAGAGGA CGCCCACTAC CCCCCGCACG ACCCCCACCA TGAGCTGGGG CCTGGGTCCA 
CAGAGCGAGG TCGGGGCCTG CCATCAGCCC TCTTCTCCCC CCCCACCCAA GCTGGACAAG 
CGGAAACTGG TGGATGCTGA TGGCCTCATC CTGCCCGAGC TCTTCTACGT TGGGCTGACC 
GTGTGGGTGA GCAGAGACCC TTTGGGCCTG GCAGCCTCCC AGGCCAACTT CTACCCCCCG 
CCCCCCGAGT GGCTCCACGA CAAATATGAC AGCACTGGGG AGAGCTTGCA CATCCCTGCG 
GCCCAGCCTT TGGAGTTTGC CCAGTTCCCC TTCCTGCTGA GCGGGCTCCG CCAGACGGCA 
GACTTCGTGG AGGCCATCGA GGGGGCCCGG GCGGCCTGCG CAGAGGCGGC CCGCGCTGGG 
ATCCGGGCCT ACCCCAGCGG CTCCCCGTTC CTCTTCTGGG AGCAGTACTT GGGCCTCCGC 
CGCTGCTTTC TGCTGGCCGT CTGCGTGCTG CTGGCCTGCA CCTTCGTGGT CTGTGCCTTG 
CTGCTGCTCA GCCCCTGGAC CGCCGGCCTC ATTGTGCTGG TGCTGGCCAT GATGACTGTG 
GAATTATTCG GCATCATGGG CTTCCTGGGC ATCAAACTCA GCGCCATCCC CGTGGTGATT 
CTCGTGGCCT CCGTGGGCAT TGGCGTCGAG TTCACAGCTC ACGTGGCTCT GGGCTTCCTG 
ACAGCCACCG GCAGCCGGGA CGCGCGGTCA GCCCAGGCCT TGGAGCACAT GTTTGCGCCC 
GTGATGGATG GGGCCGTGTC CACGCTGCTG GGCCTGCTCA TGCTGGCCGG CTCGGACTTC 
GACTTTATTG TCAGGTACTT CTTTGTGGTG CTGACAGTGC TGACCGGCCT GGGCCTGCTT 
CACGGGCTTG TTCTGCTTCC TGTGCTGCTG TCCATCATAG GACCCCCACC CCAGGTACCG 
CTGCCAGATG GAGGCGGCCG CCCGCCCTGC CCTGACCCGG TATCGCTGCC CCTCAGTCCT 
CCTCACTTCT TCCTGGGCCC GAGCCCAGCC TTCAGGGCTC CTGAGACAGG AGCAGGGGAC 
GCCCCAAGCA CCTTCATCCT GCCCCCGGCC CACTCCCACA TCTTGGTGGA GGCCAGCAAG 
GACCCCAGCT TCCCGACCAT CACGGTGGTG CAGACGTACA AGGATGGTTC TCCTGGCTCC 
GGGCTACCTC CCCTTACGGC CACGGCCGGC AGTGAGGCCC GCTGGGGGCC CCATGCCAGC 
CCCGGCGCCT TCACCACCGT GACGGCCTCC GTCACCGTGG CCCTGCACCC GCCGCCACTG 
CCGGGTAGCT ACGTCCAGGA GGTCCCTGAG GAAGCCAGGC CCCCGATGGC CGGCGAGTCC 
AAGGGACCTG GCCCCCGCTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PTCH2 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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