DENND5A cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	DENND5A
Entrez Gene ID	100916230
Full Name	DENN domain containing 5A
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023506255.1	XP_023362023.1	DENN domain-containing protein 5A
XM_031941995.1	XP_031797855.1	DENN domain-containing protein 5A isoform X1
XM_031941996.1	XP_031797856.1	DENN domain-containing protein 5A isoform X2
XM_031941998.1	XP_031797858.1	DENN domain-containing protein 5A isoform X4
XM_012552482.1	XP_012407936.1	DENN domain-containing protein 5A
XM_031941997.1	XP_031797857.1	DENN domain-containing protein 5A isoform X3

Pathways from BioSystems
Gene Ontology

The following DENND5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa135839	XM_012552482.1 Latest version!	Sarcophilus harrisii DENN/MADD domain containing 5A (DENND5A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa157434	XM_023506255.1 Latest version!	Sarcophilus harrisii DENN domain containing 5A (DENND5A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa239247	XM_031941995.1 Latest version!	Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa239248	XM_031941996.1 Latest version!	Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa239249	XM_031941997.1 Latest version!	Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa239250	XM_031941998.1 Latest version!	Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DENND5A ( XM_012552482.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012407936.1 Sarcophilus harrisii DENN/MADD domain containing 5A (DENND5A), mRNA.

Price & Availability↑

CloneID	OSa135839
Clone ID Related Accession (Same CDS sequence)	XM_012552482.1
Accession Version	XM_012552482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DENN domain-containing protein 5A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003846890.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGACCCTCC TGTACTGCCT GGATGTGAAG CCGCCTTTGT CCAGGATGGG GGACACTGGT 
CTGACCAAGT GGGTGGGCTG GGGAGGGAGA GCTGGGTCCT TCCGGAGAGG TGAGAGGCCT 
AGGAACCCCG TGGCTGGCCC AGAGTCGGAC GGCAGTCTGG CTGTGTTTGG CTGTCACCCC 
TCGTCCCTCT ATGCTCTTGT AACATTCTGT TGTTTCATTT GTCACCTAGC TTTATGCCAG 
TACATACGGG CTTCTAAAGC CAGGGATGGC AGCAGCCCTT TCATTGCCAG TACAGCAGAA 
GGAGAAAATT TTGAGCAGAC ACCCCTGAGA CGGACATTCA AATCTAAGGT TCTTGCCCGA 
TACCCTGAGA ATGTAGAATG GAATCCTTTT GACCAAGATG CGGTGGGAAT GTTGTGCATG 
CCCAAAGGGC TGGCGTTCAA GACGCAGGCT GATCCCAGGA GCCCCCAGTT CCACGCCTTC 
ATCATCACGC GGGAGGACGG CTCCCGGACG TTCGGGTTTG CCCTCACCTT TTACGAGGAG 
GTGACCAGCA AGCAGATCTG CAGTGCGATG CAGACGCTCT ACCACATGCA CAACGCCGAG 
TACGACGGGC TCCGCGCGCC CGCTGCCAAC GGCCGCGAGG GCGGCGCGCC CCAGGACGGC 
TGTGACGGTG CGCCCGGCTC CAAGCTGCAG CGCTTCAACT CGTACGACAT CAGCCGGGAC 
ACGCTCTACG TCTCCAAGTG CATCTGCCTC ATTGCGCCCA TGTCTTTCAT GAAGGCGTGC 
CGGCGGGCAC TGGAGCAGCT CCACCAGGCC GTGACCTCCC CGCAGCCCCC GCCGCTGCCC 
CTCGAGAGCT ACATCTACAA CATCCTCTAC GAGGTGCCCC TGCCCCCGCC GGGCAGGTCC 
CTGAAGTTCT CCGGCGTCTA CGGGCCCATC GTCTGCCAGA GGCCCAGCAC CAGTGAGCTG 
CCGCTCTTCG ACTTCCCCGT GCGCGAGGTC TTCGAGCTGC TTGGCGTGGA GAATGTGCTC 
CAGCTCTTCA CCTGCGCCCT GCTGGAGTTT CAGATCCTGC TCTACTCCCA GCATTACCAG 
AGACTGATGA CTGTGGCCGA GACCATCACA GCGCTCATGT TCCCTTTCCA GTGGCAGCAC 
GTCTACGTCC CCATCCTCCC CGCCTCTCTC CTGCACTTCC TAGATGCTCC TGTTCCGTAC 
CTGATGGGCT TGCACTCCAA CGGCCTGGAT GACCGCTCCA AACTAGAGTT GCCTCAAGAG 
GCCAATCTCT GCTTTGTGGA CATTGACAAC CATTTCATTG AGCTGCCCGA GGACCTGCCT 
CAGTTTCCCA ACAAGCTAGA GTTTGTCCAG GAGATCTCTG AGATTCTTAT GGCCTTTGGA 
ATTCCCCCAG AAGGGAACCT GCACTGCAGC GAGAGTGCCT CCAAATTGAA GAGCCTCAGG 
GCCTGTGACA TTGCCTCAGA CAAAAGAAAT GGGAACATTG CCAGCTCCCC TCTGCACACC 
TATGGACTCC TCAAGGAGAA TGAGACCATT GCCAGGCTGC AAGCCCTGGT CCAAAGGACT 
GGGGTAAGCT TGGAAAAGCT GGACATGCGA GAAGAGAGCA GCAGCAATAA GGATCTCAAA 
GTTCAGTGTG AGGAAGAGGA GCTCAGGGTT TACCAGCTGA ACATCCAGAT CCGAGAAGTC 
TTTGCCAACC GCTTCACCCA GATGTTTGCC GACTATGAAG TGTTTGTCAT CCAGCCCAGC 
CAGGATAAAG AGTCCTGGTT CACCAATAGG GAGCAGATGC AGAACTTTGA TAAGGCCTCT 
TTTTTATCAG ATCAGCCTGA GCCCTACCTG CCATTCCTCT CAAGGTTCCT GGAGACCCAG 
ATGTTTGCAT CCTTTGTTGA CAATAAGATC ATGTGCCACG ATGATGATGA CAAAGACCCC 
ATTCTGAGGG TGTTTGATAC TCGAGTGGAC AAGATAAGGT TACTGAACGT GAGGACGCCG 
ACCCTGCGAA CCTCAATGTA CCAGAAGTGC ACAACTGTGG ATGAAGCAGA GAAAGCGATT 
GAGCTGAGAC TGGCAAAAAT CGACCACACT GCTATTCACC CCCACTTATT GGACATGAAG 
ATTGGACAGG GGAAGTATGA GCCGGGATTC TTCCCCAAAC TGCAGGCTGA TGTGCTTTCA 
ACCGGGCCAG CCAGCAACAA GTGGACCAAG AGGAATGCAC CTGCCCAGTG GAGGCGGAAG 
GATAGACAGA AGCAGCACAC AGAACATCTA CGCCTAGATA ATGACCAGAG GGAGAAGTAC 
ATCCAGGAAG CCAGGAACAT GGGCAGCACG ATCCGGCAGC CCAAGCTGTC CAATCTCTCG 
CCCTCCGTGA TCGCCCAGAC CAACTGGAAG TTCGTGGAGG GCTTGCTGAA GGAGTGCCGC 
AATAAGACCA AGAGGATGCT AGTGGAAAAG ATGGGCCGAG AGGCTGTGGA GCTGGGCCAT 
GGAGAAGTCA ACATCACCGG TGTGGAGGAG AACACTTTGA TTGCTAGCCT TTGTGACCTC 
CTAGAAAGGA TCTGGAGCCA CGGGCTTCAG GTCAAACAGG GGAAGTCTGC CTTGTGGTCC 
CACTTGTTGC ACTATCAGGA AAATCGGCAG AGGAAGCTGA CGGCGGGAAG CTTGAGTACC 
TCAGGGATAC TGCTGGACTC GGAGCGGAGG AAGAGTGACG CCAGCTCGGC CATGCCGCCC 
CTGAGGATCT CTCTCATCCA GGACATGAGG CACATCCAGA ACATTGGGGA GATCAAAACG 
GACGTTGGCA AGGCCAGGGC CTGGGTCCGG CTGAGCATGG AGAAGAAGTT ACTTTCCCGG 
CACCTGAAGC AGTTGCTCTC AGATCACGAG CTCACCAAGA AGCTGTACAA GCGCTATGCT 
TTCCTGCGCT GCGACGACGA GAAGGAGCAG TTCCTCTATC ACCTCCTGTC CTTCAACGCT 
GTGGACTACT TCTGCTTTAC CAACGTCTTC ACGACAATCA TGATCCCGTA CCACATCCTG 
ATTGTCCCCA GCAAGAAGTT GGGCGGCTCC ATGTTCACGG CCAACCCTTG GATCTGCATC 
TCAGGAGAGC TGGGGGAGAC GCAGATCCTA CAGGTCCCCA GGAACGTGCT GGAGATGACC 
TTTGAGTACC AGAACCTGGG CAAGCTCACC ACGGTCCAGA TCGGCCATGA TAACTCGGGG 
CTGTACGCCA AGTGGCTGGT GGAGCACGTG ATGGTCAGGA ACGAGATCAC GGGACACACA 
TACAAGTTCC CCTGTGGCCG GTGGCTGGGG AAGGGCATGG ACGATGGCAG CCTGGAGAGG 
GTCCTGGTTG GGGAGCTGCT GACCTCTCAC CCCGAAGCTG ACGAGAGGCA GTGCCGAACG 
CCGCCCCTGC AGCAGTCTCC CAGCATGATC CGGAGGCTGG TCACCATCTC CCCCAGTAAC 
AAGCCCAAGT TAAACACGGG GCAGATCCAG GAGTCCATTG GGGAGGCCGT CAATGGCATT 
GTCAAGCACT TCCATAAGCC AGAGAAAGAG CGTGGCAGCC TGACGCTCCT GCTCTGTGGC 
GAGTGTGGCC TGGTATCTGC CCTGGAGCAA GTTTTTCAGC ATGGATTTAA ATCTCCCCGG 
CTCTTCAAGA ACGTCTTCAT CTGGGATTTT CTGGAAAAAG CACAAACCTA CTACGAGACC 
CTCGACCAGA GTGACCTCGT ACCTGAGGAG AACTGGCAGA CGAGGGCCCG AAACTTCTGC 
CGCTTTGTCA CCGCCATCAA CAACACCCCC CGCAACATCG GCAAAGATGG CAAGTTCCAG 
ATGCTGGTGT GTCTGGGAGC CAGAGATCAC CTCTTGCACC ACTGGATTGC CCTGCTGGCC 
GACTGTCCGA TCACAGCACA GATGTATGAA GATGTGGCGC TCCTCAAGGA CCACACGCTT 
GTGAATTCCT TGATCCGGGT CCTGCAGACT TTGCAGGAGT TCAATATCAC ACTGGAGACG 
TCCCTGGTCA AAGGGATAGA CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012407936.1
CDS	1..3987
Translation	MTLLYCLDVKPPLSRMGDTGLTKWVGWGGRAGSFRRGERPRNPVAGPESDGSLAVFGCHPSSLYALVTFCCFICHLALCQYIRASKARDGSSPFIASTAEGENFEQTPLRRTFKSKVLARYPENVEWNPFDQDAVGMLCMPKGLAFKTQADPRSPQFHAFIITREDGSRTFGFALTFYEEVTSKQICSAMQTLYHMHNAEYDGLRAPAANGREGGAPQDGCDGAPGSKLQRFNSYDISRDTLYVSKCICLIAPMSFMKACRRALEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPPGRSLKFSGVYGPIVCQRPSTSELPLFDFPVREVFELLGVENVLQLFTCALLEFQILLYSQHYQRLMTVAETITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFVQEISEILMAFGIPPEGNLHCSESASKLKSLRACDIASDKRNGNIASSPLHTYGLLKENETIARLQALVQRTGVSLEKLDMREESSSNKDLKVQCEEEELRVYQLNIQIREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFVDNKIMCHDDDDKDPILRVFDTRVDKIRLLNVRTPTLRTSMYQKCTTVDEAEKAIELRLAKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQADVLSTGPASNKWTKRNAPAQWRRKDRQKQHTEHLRLDNDQREKYIQEARNMGSTIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVNITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQENRQRKLTAGSLSTSGILLDSERRKSDASSAMPPLRISLIQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETQILQVPRNVLEMTFEYQNLGKLTTVQIGHDNSGLYAKWLVEHVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGELLTSHPEADERQCRTPPLQQSPSMIRRLVTISPSNKPKLNTGQIQESIGEAVNGIVKHFHKPEKERGSLTLLLCGECGLVSALEQVFQHGFKSPRLFKNVFIWDFLEKAQTYYETLDQSDLVPEENWQTRARNFCRFVTAINNTPRNIGKDGKFQMLVCLGARDHLLHHWIALLADCPITAQMYEDVALLKDHTLVNSLIRVLQTLQEFNITLETSLVKGIDI

Target ORF information:

RefSeq Version	XM_012552482.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii DENN/MADD domain containing 5A (DENND5A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012552482.1

ORF Insert Sequence:

ATGACCCTCC TGTACTGCCT GGATGTGAAG CCGCCTTTGT CCAGGATGGG GGACACTGGT 
CTGACCAAGT GGGTGGGCTG GGGAGGGAGA GCTGGGTCCT TCCGGAGAGG TGAGAGGCCT 
AGGAACCCCG TGGCTGGCCC AGAGTCGGAC GGCAGTCTGG CTGTGTTTGG CTGTCACCCC 
TCGTCCCTCT ATGCTCTTGT AACATTCTGT TGTTTCATTT GTCACCTAGC TTTATGCCAG 
TACATACGGG CTTCTAAAGC CAGGGATGGC AGCAGCCCTT TCATTGCCAG TACAGCAGAA 
GGAGAAAATT TTGAGCAGAC ACCCCTGAGA CGGACATTCA AATCTAAGGT TCTTGCCCGA 
TACCCTGAGA ATGTAGAATG GAATCCTTTT GACCAAGATG CGGTGGGAAT GTTGTGCATG 
CCCAAAGGGC TGGCGTTCAA GACGCAGGCT GATCCCAGGA GCCCCCAGTT CCACGCCTTC 
ATCATCACGC GGGAGGACGG CTCCCGGACG TTCGGGTTTG CCCTCACCTT TTACGAGGAG 
GTGACCAGCA AGCAGATCTG CAGTGCGATG CAGACGCTCT ACCACATGCA CAACGCCGAG 
TACGACGGGC TCCGCGCGCC CGCTGCCAAC GGCCGCGAGG GCGGCGCGCC CCAGGACGGC 
TGTGACGGTG CGCCCGGCTC CAAGCTGCAG CGCTTCAACT CGTACGACAT CAGCCGGGAC 
ACGCTCTACG TCTCCAAGTG CATCTGCCTC ATTGCGCCCA TGTCTTTCAT GAAGGCGTGC 
CGGCGGGCAC TGGAGCAGCT CCACCAGGCC GTGACCTCCC CGCAGCCCCC GCCGCTGCCC 
CTCGAGAGCT ACATCTACAA CATCCTCTAC GAGGTGCCCC TGCCCCCGCC GGGCAGGTCC 
CTGAAGTTCT CCGGCGTCTA CGGGCCCATC GTCTGCCAGA GGCCCAGCAC CAGTGAGCTG 
CCGCTCTTCG ACTTCCCCGT GCGCGAGGTC TTCGAGCTGC TTGGCGTGGA GAATGTGCTC 
CAGCTCTTCA CCTGCGCCCT GCTGGAGTTT CAGATCCTGC TCTACTCCCA GCATTACCAG 
AGACTGATGA CTGTGGCCGA GACCATCACA GCGCTCATGT TCCCTTTCCA GTGGCAGCAC 
GTCTACGTCC CCATCCTCCC CGCCTCTCTC CTGCACTTCC TAGATGCTCC TGTTCCGTAC 
CTGATGGGCT TGCACTCCAA CGGCCTGGAT GACCGCTCCA AACTAGAGTT GCCTCAAGAG 
GCCAATCTCT GCTTTGTGGA CATTGACAAC CATTTCATTG AGCTGCCCGA GGACCTGCCT 
CAGTTTCCCA ACAAGCTAGA GTTTGTCCAG GAGATCTCTG AGATTCTTAT GGCCTTTGGA 
ATTCCCCCAG AAGGGAACCT GCACTGCAGC GAGAGTGCCT CCAAATTGAA GAGCCTCAGG 
GCCTGTGACA TTGCCTCAGA CAAAAGAAAT GGGAACATTG CCAGCTCCCC TCTGCACACC 
TATGGACTCC TCAAGGAGAA TGAGACCATT GCCAGGCTGC AAGCCCTGGT CCAAAGGACT 
GGGGTAAGCT TGGAAAAGCT GGACATGCGA GAAGAGAGCA GCAGCAATAA GGATCTCAAA 
GTTCAGTGTG AGGAAGAGGA GCTCAGGGTT TACCAGCTGA ACATCCAGAT CCGAGAAGTC 
TTTGCCAACC GCTTCACCCA GATGTTTGCC GACTATGAAG TGTTTGTCAT CCAGCCCAGC 
CAGGATAAAG AGTCCTGGTT CACCAATAGG GAGCAGATGC AGAACTTTGA TAAGGCCTCT 
TTTTTATCAG ATCAGCCTGA GCCCTACCTG CCATTCCTCT CAAGGTTCCT GGAGACCCAG 
ATGTTTGCAT CCTTTGTTGA CAATAAGATC ATGTGCCACG ATGATGATGA CAAAGACCCC 
ATTCTGAGGG TGTTTGATAC TCGAGTGGAC AAGATAAGGT TACTGAACGT GAGGACGCCG 
ACCCTGCGAA CCTCAATGTA CCAGAAGTGC ACAACTGTGG ATGAAGCAGA GAAAGCGATT 
GAGCTGAGAC TGGCAAAAAT CGACCACACT GCTATTCACC CCCACTTATT GGACATGAAG 
ATTGGACAGG GGAAGTATGA GCCGGGATTC TTCCCCAAAC TGCAGGCTGA TGTGCTTTCA 
ACCGGGCCAG CCAGCAACAA GTGGACCAAG AGGAATGCAC CTGCCCAGTG GAGGCGGAAG 
GATAGACAGA AGCAGCACAC AGAACATCTA CGCCTAGATA ATGACCAGAG GGAGAAGTAC 
ATCCAGGAAG CCAGGAACAT GGGCAGCACG ATCCGGCAGC CCAAGCTGTC CAATCTCTCG 
CCCTCCGTGA TCGCCCAGAC CAACTGGAAG TTCGTGGAGG GCTTGCTGAA GGAGTGCCGC 
AATAAGACCA AGAGGATGCT AGTGGAAAAG ATGGGCCGAG AGGCTGTGGA GCTGGGCCAT 
GGAGAAGTCA ACATCACCGG TGTGGAGGAG AACACTTTGA TTGCTAGCCT TTGTGACCTC 
CTAGAAAGGA TCTGGAGCCA CGGGCTTCAG GTCAAACAGG GGAAGTCTGC CTTGTGGTCC 
CACTTGTTGC ACTATCAGGA AAATCGGCAG AGGAAGCTGA CGGCGGGAAG CTTGAGTACC 
TCAGGGATAC TGCTGGACTC GGAGCGGAGG AAGAGTGACG CCAGCTCGGC CATGCCGCCC 
CTGAGGATCT CTCTCATCCA GGACATGAGG CACATCCAGA ACATTGGGGA GATCAAAACG 
GACGTTGGCA AGGCCAGGGC CTGGGTCCGG CTGAGCATGG AGAAGAAGTT ACTTTCCCGG 
CACCTGAAGC AGTTGCTCTC AGATCACGAG CTCACCAAGA AGCTGTACAA GCGCTATGCT 
TTCCTGCGCT GCGACGACGA GAAGGAGCAG TTCCTCTATC ACCTCCTGTC CTTCAACGCT 
GTGGACTACT TCTGCTTTAC CAACGTCTTC ACGACAATCA TGATCCCGTA CCACATCCTG 
ATTGTCCCCA GCAAGAAGTT GGGCGGCTCC ATGTTCACGG CCAACCCTTG GATCTGCATC 
TCAGGAGAGC TGGGGGAGAC GCAGATCCTA CAGGTCCCCA GGAACGTGCT GGAGATGACC 
TTTGAGTACC AGAACCTGGG CAAGCTCACC ACGGTCCAGA TCGGCCATGA TAACTCGGGG 
CTGTACGCCA AGTGGCTGGT GGAGCACGTG ATGGTCAGGA ACGAGATCAC GGGACACACA 
TACAAGTTCC CCTGTGGCCG GTGGCTGGGG AAGGGCATGG ACGATGGCAG CCTGGAGAGG 
GTCCTGGTTG GGGAGCTGCT GACCTCTCAC CCCGAAGCTG ACGAGAGGCA GTGCCGAACG 
CCGCCCCTGC AGCAGTCTCC CAGCATGATC CGGAGGCTGG TCACCATCTC CCCCAGTAAC 
AAGCCCAAGT TAAACACGGG GCAGATCCAG GAGTCCATTG GGGAGGCCGT CAATGGCATT 
GTCAAGCACT TCCATAAGCC AGAGAAAGAG CGTGGCAGCC TGACGCTCCT GCTCTGTGGC 
GAGTGTGGCC TGGTATCTGC CCTGGAGCAA GTTTTTCAGC ATGGATTTAA ATCTCCCCGG 
CTCTTCAAGA ACGTCTTCAT CTGGGATTTT CTGGAAAAAG CACAAACCTA CTACGAGACC 
CTCGACCAGA GTGACCTCGT ACCTGAGGAG AACTGGCAGA CGAGGGCCCG AAACTTCTGC 
CGCTTTGTCA CCGCCATCAA CAACACCCCC CGCAACATCG GCAAAGATGG CAAGTTCCAG 
ATGCTGGTGT GTCTGGGAGC CAGAGATCAC CTCTTGCACC ACTGGATTGC CCTGCTGGCC 
GACTGTCCGA TCACAGCACA GATGTATGAA GATGTGGCGC TCCTCAAGGA CCACACGCTT 
GTGAATTCCT TGATCCGGGT CCTGCAGACT TTGCAGGAGT TCAATATCAC ACTGGAGACG 
TCCCTGGTCA AAGGGATAGA CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND5A ( XM_023506255.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023362023.1 Sarcophilus harrisii DENN domain containing 5A (DENND5A), mRNA.

Price & Availability↑

CloneID	OSa157434
Clone ID Related Accession (Same CDS sequence)	XM_023506255.1
Accession Version	XM_023506255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DENN domain-containing protein 5A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003846890.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGCTGAGG ATATCTCTGT GGATAGCCAA GCGGATGGCA TTCCCCTTCA GGCTCCTGTT 
TTTGTGGTTA TTGGCTGTGG GTCTATGACC CTCCTGTACT GCCTGGATGT GAAGCCGCCT 
TTGTCCAGGA TGGGGGACAC TGGTCTGACC AAGTGGGTTT GTTTCAGCTC TAAATCCTCG 
GGCCTGGGAC TTGGACATTT AGTAAAACAC TCTTTATGCC AGTACATACG GGCTTCTAAA 
GCCAGGGATG GCAGCAGCCC TTTCATTGCC AGTACAGCAG AAGGAGAAAA TTTTGAGCAG 
ACACCCCTGA GACGGACATT CAAATCTAAG GTTCTTGCCC GATACCCTGA GAATGTAGAA 
TGGAATCCTT TTGACCAAGA TGCGGTGGGA ATGTTGTGCA TGCCCAAAGG GCTGGCGTTC 
AAGACGCAGG CTGATCCCAG GAGCCCCCAG TTCCACGCCT TCATCATCAC GCGGGAGGAC 
GGCTCCCGGA CGTTCGGGTT TGCCCTCACC TTTTACGAGG AGGTGACCAG CAAGCAGATC 
TGCAGTGCGA TGCAGACGCT CTACCACATG CACAACGCCG AGTACGACGG GCTCCGCGCG 
CCCGCTGCCA ACGGCCGCGA GGGCGGCGCG CCCCAGGACG GCTGTGACGG TGCGCCCGGC 
TCCAAGCTGC AGCGCTTCAA CTCGTACGAC ATCAGCCGGG ACACGCTCTA CGTCTCCAAG 
TGCATCTGCC TCATTGCGCC CATGTCTTTC ATGAAGGCGT GCCGGCGGGC ACTGGAGCAG 
CTCCACCAGG CCGTGACCTC CCCGCAGCCC CCGCCGCTGC CCCTCGAGAG CTACATCTAC 
AACATCCTCT ACGAGGTGCC CCTGCCCCCG CCGGGCAGGT CCCTGAAGTT CTCCGGCGTC 
TACGGGCCCA TCGTCTGCCA GAGGCCCAGC ACCAGTGAGC TGCCGCTCTT CGACTTCCCC 
GTGCGCGAGG TCTTCGAGCT GCTTGGCGTG GAGAATGTGC TCCAGCTCTT CACCTGCGCC 
CTGCTGGAGT TTCAGATCCT GCTCTACTCC CAGCATTACC AGAGACTGAT GACTGTGGCC 
GAGACCATCA CAGCGCTCAT GTTCCCTTTC CAGTGGCAGC ACGTCTACGT CCCCATCCTC 
CCCGCCTCTC TCCTGCACTT CCTAGATGCT CCTGTTCCGT ACCTGATGGG CTTGCACTCC 
AACGGCCTGG ATGACCGCTC CAAACTAGAG TTGCCTCAAG AGGCCAATCT CTGCTTTGTG 
GACATTGACA ACCATTTCAT TGAGCTGCCC GAGGACCTGC CTCAGTTTCC CAACAAGCTA 
GAGTTTGTCC AGGAGATCTC TGAGATTCTT ATGGCCTTTG GAATTCCCCC AGAAGGGAAC 
CTGCACTGCA GCGAGAGTGC CTCCAAATTG AAGAGCCTCA GGGCCTGTGA CATTGCCTCA 
GACAAAAGAA ATGGGAACAT TGCCAGCTCC CCTCTGCACA CCTATGGACT CCTCAAGGAG 
AATGAGACCA TTGCCAGGCT GCAAGCCCTG GTCCAAAGGA CTGGGGTAAG CTTGGAAAAG 
CTGGACATGC GAGAAGAGAG CAGCAGCAAT AAGGATCTCA AAGTTCAGTG TGAGGAAGAG 
GAGCTCAGGG TTTACCAGCT GAACATCCAG ATCCGAGAAG TCTTTGCCAA CCGCTTCACC 
CAGATGTTTG CCGACTATGA AGTGTTTGTC ATCCAGCCCA GCCAGGATAA AGAGTCCTGG 
TTCACCAATA GGGAGCAGAT GCAGAACTTT GATAAGGCCT CTTTTTTATC AGATCAGCCT 
GAGCCCTACC TGCCATTCCT CTCAAGGTTC CTGGAGACCC AGATGTTTGC ATCCTTTGTT 
GACAATAAGA TCATGTGCCA CGATGATGAT GACAAAGACC CCATTCTGAG GGTGTTTGAT 
ACTCGAGTGG ACAAGATAAG GTTACTGAAC GTGAGGACGC CGACCCTGCG AACCTCAATG 
TACCAGAAGT GCACAACTGT GGATGAAGCA GAGAAAGCGA TTGAGCTGAG ACTGGCAAAA 
ATCGACCACA CTGCTATTCA CCCCCACTTA TTGGACATGA AGATTGGACA GGGGAAGTAT 
GAGCCGGGAT TCTTCCCCAA ACTGCAGGCT GATGTGCTTT CAACCGGGCC AGCCAGCAAC 
AAGTGGACCA AGAGGAATGC ACCTGCCCAG TGGAGGCGGA AGGATAGACA GAAGCAGCAC 
ACAGAACATC TACGCCTAGA TAATGACCAG AGGGAGAAGT ACATCCAGGA AGCCAGGAAC 
ATGGGCAGCA CGATCCGGCA GCCCAAGCTG TCCAATCTCT CGCCCTCCGT GATCGCCCAG 
ACCAACTGGA AGTTCGTGGA GGGCTTGCTG AAGGAGTGCC GCAATAAGAC CAAGAGGATG 
CTAGTGGAAA AGATGGGCCG AGAGGCTGTG GAGCTGGGCC ATGGAGAAGT CAACATCACC 
GGTGTGGAGG AGAACACTTT GATTGCTAGC CTTTGTGACC TCCTAGAAAG GATCTGGAGC 
CACGGGCTTC AGGTCAAACA GGGGAAGTCT GCCTTGTGGT CCCACTTGTT GCACTATCAG 
GAAAATCGGC AGAGGAAGCT GACGGCGGGA AGCTTGAGTA CCTCAGGGAT ACTGCTGGAC 
TCGGAGCGGA GGAAGAGTGA CGCCAGCTCG GCCATGCCGC CCCTGAGGAT CTCTCTCATC 
CAGGACATGA GGCACATCCA GAACATTGGG GAGATCAAAA CGGACGTTGG CAAGGCCAGG 
GCCTGGGTCC GGCTGAGCAT GGAGAAGAAG TTACTTTCCC GGCACCTGAA GCAGTTGCTC 
TCAGATCACG AGCTCACCAA GAAGCTGTAC AAGCGCTATG CTTTCCTGCG CTGCGACGAC 
GAGAAGGAGC AGTTCCTCTA TCACCTCCTG TCCTTCAACG CTGTGGACTA CTTCTGCTTT 
ACCAACGTCT TCACGACAAT CATGATCCCG TACCACATCC TGATTGTCCC CAGCAAGAAG 
TTGGGCGGCT CCATGTTCAC GGCCAACCCT TGGATCTGCA TCTCAGGAGA GCTGGGGGAG 
ACGCAGATCC TACAGGTCCC CAGGAACGTG CTGGAGATGA CCTTTGAGTA CCAGAACCTG 
GGCAAGCTCA CCACGGTCCA GATCGGCCAT GATAACTCGG GGCTGTACGC CAAGTGGCTG 
GTGGAGCACG TGATGGTCAG GAACGAGATC ACGGGACACA CATACAAGTT CCCCTGTGGC 
CGGTGGCTGG GGAAGGGCAT GGACGATGGC AGCCTGGAGA GGGTCCTGGT TGGGGAGCTG 
CTGACCTCTC ACCCCGAAGC TGACGAGAGG CAGTGCCGAA CGCCGCCCCT GCAGCAGTCT 
CCCAGCATGA TCCGGAGGCT GGTCACCATC TCCCCCAGTA ACAAGCCCAA GTTAAACACG 
GGGCAGATCC AGGAGTCCAT TGGGGAGGCC GTCAATGGCA TTGTCAAGCA CTTCCATAAG 
CCAGAGAAAG AGCGTGGCAG CCTGACGCTC CTGCTCTGTG GCGAGTGTGG CCTGGTATCT 
GCCCTGGAGC AAGTTTTTCA GCATGGATTT AAATCTCCCC GGCTCTTCAA GAACGTCTTC 
ATCTGGGATT TTCTGGAAAA AGCACAAACC TACTACGAGA CCCTCGACCA GAGTGACCTC 
GTACCTGAGG AGAACTGGCA GACGAGGGCC CGAAACTTCT GCCGCTTTGT CACCGCCATC 
AACAACACCC CCCGCAACAT CGGCAAAGAT GGCAAGTTCC AGATGCTGGT GTGTCTGGGA 
GCCAGAGATC ACCTCTTGCA CCACTGGATT GCCCTGCTGG CCGACTGTCC GATCACAGCA 
CAGATGTATG AAGATGTGGC GCTCCTCAAG GACCACACGC TTGTGAATTC CTTGATCCGG 
GTCCTGCAGA CTTTGCAGGA GTTCAATATC ACACTGGAGA CGTCCCTGGT CAAAGGGATA 
GACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023362023.1
CDS	1..3969
Translation	MAEDISVDSQADGIPLQAPVFVVIGCGSMTLLYCLDVKPPLSRMGDTGLTKWVCFSSKSSGLGLGHLVKHSLCQYIRASKARDGSSPFIASTAEGENFEQTPLRRTFKSKVLARYPENVEWNPFDQDAVGMLCMPKGLAFKTQADPRSPQFHAFIITREDGSRTFGFALTFYEEVTSKQICSAMQTLYHMHNAEYDGLRAPAANGREGGAPQDGCDGAPGSKLQRFNSYDISRDTLYVSKCICLIAPMSFMKACRRALEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPPGRSLKFSGVYGPIVCQRPSTSELPLFDFPVREVFELLGVENVLQLFTCALLEFQILLYSQHYQRLMTVAETITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFVQEISEILMAFGIPPEGNLHCSESASKLKSLRACDIASDKRNGNIASSPLHTYGLLKENETIARLQALVQRTGVSLEKLDMREESSSNKDLKVQCEEEELRVYQLNIQIREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFVDNKIMCHDDDDKDPILRVFDTRVDKIRLLNVRTPTLRTSMYQKCTTVDEAEKAIELRLAKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQADVLSTGPASNKWTKRNAPAQWRRKDRQKQHTEHLRLDNDQREKYIQEARNMGSTIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVNITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQENRQRKLTAGSLSTSGILLDSERRKSDASSAMPPLRISLIQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETQILQVPRNVLEMTFEYQNLGKLTTVQIGHDNSGLYAKWLVEHVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGELLTSHPEADERQCRTPPLQQSPSMIRRLVTISPSNKPKLNTGQIQESIGEAVNGIVKHFHKPEKERGSLTLLLCGECGLVSALEQVFQHGFKSPRLFKNVFIWDFLEKAQTYYETLDQSDLVPEENWQTRARNFCRFVTAINNTPRNIGKDGKFQMLVCLGARDHLLHHWIALLADCPITAQMYEDVALLKDHTLVNSLIRVLQTLQEFNITLETSLVKGIDI

Target ORF information:

RefSeq Version	XM_023506255.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii DENN domain containing 5A (DENND5A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023506255.1

ORF Insert Sequence:

ATGGCTGAGG ATATCTCTGT GGATAGCCAA GCGGATGGCA TTCCCCTTCA GGCTCCTGTT 
TTTGTGGTTA TTGGCTGTGG GTCTATGACC CTCCTGTACT GCCTGGATGT GAAGCCGCCT 
TTGTCCAGGA TGGGGGACAC TGGTCTGACC AAGTGGGTTT GTTTCAGCTC TAAATCCTCG 
GGCCTGGGAC TTGGACATTT AGTAAAACAC TCTTTATGCC AGTACATACG GGCTTCTAAA 
GCCAGGGATG GCAGCAGCCC TTTCATTGCC AGTACAGCAG AAGGAGAAAA TTTTGAGCAG 
ACACCCCTGA GACGGACATT CAAATCTAAG GTTCTTGCCC GATACCCTGA GAATGTAGAA 
TGGAATCCTT TTGACCAAGA TGCGGTGGGA ATGTTGTGCA TGCCCAAAGG GCTGGCGTTC 
AAGACGCAGG CTGATCCCAG GAGCCCCCAG TTCCACGCCT TCATCATCAC GCGGGAGGAC 
GGCTCCCGGA CGTTCGGGTT TGCCCTCACC TTTTACGAGG AGGTGACCAG CAAGCAGATC 
TGCAGTGCGA TGCAGACGCT CTACCACATG CACAACGCCG AGTACGACGG GCTCCGCGCG 
CCCGCTGCCA ACGGCCGCGA GGGCGGCGCG CCCCAGGACG GCTGTGACGG TGCGCCCGGC 
TCCAAGCTGC AGCGCTTCAA CTCGTACGAC ATCAGCCGGG ACACGCTCTA CGTCTCCAAG 
TGCATCTGCC TCATTGCGCC CATGTCTTTC ATGAAGGCGT GCCGGCGGGC ACTGGAGCAG 
CTCCACCAGG CCGTGACCTC CCCGCAGCCC CCGCCGCTGC CCCTCGAGAG CTACATCTAC 
AACATCCTCT ACGAGGTGCC CCTGCCCCCG CCGGGCAGGT CCCTGAAGTT CTCCGGCGTC 
TACGGGCCCA TCGTCTGCCA GAGGCCCAGC ACCAGTGAGC TGCCGCTCTT CGACTTCCCC 
GTGCGCGAGG TCTTCGAGCT GCTTGGCGTG GAGAATGTGC TCCAGCTCTT CACCTGCGCC 
CTGCTGGAGT TTCAGATCCT GCTCTACTCC CAGCATTACC AGAGACTGAT GACTGTGGCC 
GAGACCATCA CAGCGCTCAT GTTCCCTTTC CAGTGGCAGC ACGTCTACGT CCCCATCCTC 
CCCGCCTCTC TCCTGCACTT CCTAGATGCT CCTGTTCCGT ACCTGATGGG CTTGCACTCC 
AACGGCCTGG ATGACCGCTC CAAACTAGAG TTGCCTCAAG AGGCCAATCT CTGCTTTGTG 
GACATTGACA ACCATTTCAT TGAGCTGCCC GAGGACCTGC CTCAGTTTCC CAACAAGCTA 
GAGTTTGTCC AGGAGATCTC TGAGATTCTT ATGGCCTTTG GAATTCCCCC AGAAGGGAAC 
CTGCACTGCA GCGAGAGTGC CTCCAAATTG AAGAGCCTCA GGGCCTGTGA CATTGCCTCA 
GACAAAAGAA ATGGGAACAT TGCCAGCTCC CCTCTGCACA CCTATGGACT CCTCAAGGAG 
AATGAGACCA TTGCCAGGCT GCAAGCCCTG GTCCAAAGGA CTGGGGTAAG CTTGGAAAAG 
CTGGACATGC GAGAAGAGAG CAGCAGCAAT AAGGATCTCA AAGTTCAGTG TGAGGAAGAG 
GAGCTCAGGG TTTACCAGCT GAACATCCAG ATCCGAGAAG TCTTTGCCAA CCGCTTCACC 
CAGATGTTTG CCGACTATGA AGTGTTTGTC ATCCAGCCCA GCCAGGATAA AGAGTCCTGG 
TTCACCAATA GGGAGCAGAT GCAGAACTTT GATAAGGCCT CTTTTTTATC AGATCAGCCT 
GAGCCCTACC TGCCATTCCT CTCAAGGTTC CTGGAGACCC AGATGTTTGC ATCCTTTGTT 
GACAATAAGA TCATGTGCCA CGATGATGAT GACAAAGACC CCATTCTGAG GGTGTTTGAT 
ACTCGAGTGG ACAAGATAAG GTTACTGAAC GTGAGGACGC CGACCCTGCG AACCTCAATG 
TACCAGAAGT GCACAACTGT GGATGAAGCA GAGAAAGCGA TTGAGCTGAG ACTGGCAAAA 
ATCGACCACA CTGCTATTCA CCCCCACTTA TTGGACATGA AGATTGGACA GGGGAAGTAT 
GAGCCGGGAT TCTTCCCCAA ACTGCAGGCT GATGTGCTTT CAACCGGGCC AGCCAGCAAC 
AAGTGGACCA AGAGGAATGC ACCTGCCCAG TGGAGGCGGA AGGATAGACA GAAGCAGCAC 
ACAGAACATC TACGCCTAGA TAATGACCAG AGGGAGAAGT ACATCCAGGA AGCCAGGAAC 
ATGGGCAGCA CGATCCGGCA GCCCAAGCTG TCCAATCTCT CGCCCTCCGT GATCGCCCAG 
ACCAACTGGA AGTTCGTGGA GGGCTTGCTG AAGGAGTGCC GCAATAAGAC CAAGAGGATG 
CTAGTGGAAA AGATGGGCCG AGAGGCTGTG GAGCTGGGCC ATGGAGAAGT CAACATCACC 
GGTGTGGAGG AGAACACTTT GATTGCTAGC CTTTGTGACC TCCTAGAAAG GATCTGGAGC 
CACGGGCTTC AGGTCAAACA GGGGAAGTCT GCCTTGTGGT CCCACTTGTT GCACTATCAG 
GAAAATCGGC AGAGGAAGCT GACGGCGGGA AGCTTGAGTA CCTCAGGGAT ACTGCTGGAC 
TCGGAGCGGA GGAAGAGTGA CGCCAGCTCG GCCATGCCGC CCCTGAGGAT CTCTCTCATC 
CAGGACATGA GGCACATCCA GAACATTGGG GAGATCAAAA CGGACGTTGG CAAGGCCAGG 
GCCTGGGTCC GGCTGAGCAT GGAGAAGAAG TTACTTTCCC GGCACCTGAA GCAGTTGCTC 
TCAGATCACG AGCTCACCAA GAAGCTGTAC AAGCGCTATG CTTTCCTGCG CTGCGACGAC 
GAGAAGGAGC AGTTCCTCTA TCACCTCCTG TCCTTCAACG CTGTGGACTA CTTCTGCTTT 
ACCAACGTCT TCACGACAAT CATGATCCCG TACCACATCC TGATTGTCCC CAGCAAGAAG 
TTGGGCGGCT CCATGTTCAC GGCCAACCCT TGGATCTGCA TCTCAGGAGA GCTGGGGGAG 
ACGCAGATCC TACAGGTCCC CAGGAACGTG CTGGAGATGA CCTTTGAGTA CCAGAACCTG 
GGCAAGCTCA CCACGGTCCA GATCGGCCAT GATAACTCGG GGCTGTACGC CAAGTGGCTG 
GTGGAGCACG TGATGGTCAG GAACGAGATC ACGGGACACA CATACAAGTT CCCCTGTGGC 
CGGTGGCTGG GGAAGGGCAT GGACGATGGC AGCCTGGAGA GGGTCCTGGT TGGGGAGCTG 
CTGACCTCTC ACCCCGAAGC TGACGAGAGG CAGTGCCGAA CGCCGCCCCT GCAGCAGTCT 
CCCAGCATGA TCCGGAGGCT GGTCACCATC TCCCCCAGTA ACAAGCCCAA GTTAAACACG 
GGGCAGATCC AGGAGTCCAT TGGGGAGGCC GTCAATGGCA TTGTCAAGCA CTTCCATAAG 
CCAGAGAAAG AGCGTGGCAG CCTGACGCTC CTGCTCTGTG GCGAGTGTGG CCTGGTATCT 
GCCCTGGAGC AAGTTTTTCA GCATGGATTT AAATCTCCCC GGCTCTTCAA GAACGTCTTC 
ATCTGGGATT TTCTGGAAAA AGCACAAACC TACTACGAGA CCCTCGACCA GAGTGACCTC 
GTACCTGAGG AGAACTGGCA GACGAGGGCC CGAAACTTCT GCCGCTTTGT CACCGCCATC 
AACAACACCC CCCGCAACAT CGGCAAAGAT GGCAAGTTCC AGATGCTGGT GTGTCTGGGA 
GCCAGAGATC ACCTCTTGCA CCACTGGATT GCCCTGCTGG CCGACTGTCC GATCACAGCA 
CAGATGTATG AAGATGTGGC GCTCCTCAAG GACCACACGC TTGTGAATTC CTTGATCCGG 
GTCCTGCAGA CTTTGCAGGA GTTCAATATC ACACTGGAGA CGTCCCTGGT CAAAGGGATA 
GACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND5A ( XM_031941995.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031797855.1 Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X1, mRNA.

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CloneID	OSa239247
Clone ID Related Accession (Same CDS sequence)	XM_031941995.1
Accession Version	XM_031941995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DENN domain-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045431.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCGGCGGCGG CGGCGGCGGG GGCTCGCTTC CCAGCCGCTT CGCCGACTAC 
TTTGTCATCT GCGGGCTGGA CACGGAGACG GGGCTGGAGC CGGACGAGCT GTCGGCTTTA 
TGCCAGTACA TACGGGCTTC TAAAGCCAGG GATGGCAGCA GCCCTTTCAT TGCCAGTACA 
GCAGAAGGAG AAAATTTTGA GCAGACACCC CTGAGACGGA CATTCAAATC TAAGGTTCTT 
GCCCGATACC CTGAGAATGT AGAATGGAAT CCTTTTGACC AAGATGCGGT GGGAATGTTG 
TGCATGCCCA AAGGGCTGGC GTTCAAGACG CAGGCTGATC CCAGGAGCCC CCAGTTCCAC 
GCCTTCATCA TCACGCGGGA GGACGGCTCC CGGACGTTCG GGTTTGCCCT CACCTTTTAC 
GAGGAGGTGA CCAGCAAGCA GATCTGCAGT GCGATGCAGA CGCTCTACCA CATGCACAAC 
GCCGAGTACG ACGGGCTCCG CGCGCCCGCT GCCAACGGCC GCGAGGGCGG CGCGCCCCAG 
GACGGCTGTG ACGGTGCGCC CGGCTCCAAG CTGCAGCGCT TCAACTCGTA CGACATCAGC 
CGGGACACGC TCTACGTCTC CAAGTGCATC TGCCTCATTG CGCCCATGTC TTTCATGAAG 
GCGTGCCGGC GGGCACTGGA GCAGCTCCAC CAGGCCGTGA CCTCCCCGCA GCCCCCGCCG 
CTGCCCCTCG AGAGCTACAT CTACAACATC CTCTACGAGG TGCCCCTGCC CCCGCCGGGC 
AGGTCCCTGA AGTTCTCCGG CGTCTACGGG CCCATCGTCT GCCAGAGGCC CAGCACCAGT 
GAGCTGCCGC TCTTCGACTT CCCCGTGCGC GAGGTCTTCG AGCTGCTTGG CGTGGAGAAT 
GTGCTCCAGC TCTTCACCTG CGCCCTGCTG GAGTTTCAGA TCCTGCTCTA CTCCCAGCAT 
TACCAGAGAC TGATGACTGT GGCCGAGACC ATCACAGCGC TCATGTTCCC TTTCCAGTGG 
CAGCACGTCT ACGTCCCCAT CCTCCCCGCC TCTCTCCTGC ACTTCCTAGA TGCTCCTGTT 
CCGTACCTGA TGGGCTTGCA CTCCAACGGC CTGGATGACC GCTCCAAACT AGAGTTGCCT 
CAAGAGGCCA ATCTCTGCTT TGTGGACATT GACAACCATT TCATTGAGCT GCCCGAGGAC 
CTGCCTCAGT TTCCCAACAA GCTAGAGTTT GTCCAGGAGA TCTCTGAGAT TCTTATGGCC 
TTTGGAATTC CCCCAGAAGG GAACCTGCAC TGCAGCGAGA GTGCCTCCAA ATTGAAGAGC 
CTCAGGGCCT GTGACATTGC CTCAGACAAA AGAAATGGGA ACATTGCCAG CTCCCCTCTG 
CACACCTATG GACTCCTCAA GGAGAATGAG ACCATTGCCA GGCTGCAAGC CCTGGTCCAA 
AGGACTGGGG TAAGCTTGGA AAAGCTGGAC ATGCGAGAAG AGAGCAGCAG CAATAAGGAT 
CTCAAAGTTC AGTGTGAGGA AGAGGAGCTC AGGGTTTACC AGCTGAACAT CCAGATCCGA 
GAAGTCTTTG CCAACCGCTT CACCCAGATG TTTGCCGACT ATGAAGTGTT TGTCATCCAG 
CCCAGCCAGG ATAAAGAGTC CTGGTTCACC AATAGGGAGC AGATGCAGAA CTTTGATAAG 
GCCTCTTTTT TATCAGATCA GCCTGAGCCC TACCTGCCAT TCCTCTCAAG GTTCCTGGAG 
ACCCAGATGT TTGCATCCTT TGTTGACAAT AAGATCATGT GCCACGATGA TGATGACAAA 
GACCCCATTC TGAGGGTGTT TGATACTCGA GTGGACAAGA TAAGGTTACT GAACGTGAGG 
ACGCCGACCC TGCGAACCTC AATGTACCAG AAGTGCACAA CTGTGGATGA AGCAGAGAAA 
GCGATTGAGC TGAGACTGGC AAAAATCGAC CACACTGCTA TTCACCCCCA CTTATTGGAC 
ATGAAGATTG GACAGGGGAA GTATGAGCCG GGATTCTTCC CCAAACTGCA GGCTGATGTG 
CTTTCAACCG GGCCAGCCAG CAACAAGTGG ACCAAGAGGA ATGCACCTGC CCAGTGGAGG 
CGGAAGGATA GACAGAAGCA GCACACAGAA CATCTACGCC TAGATAATGA CCAGAGGGAG 
AAGTACATCC AGGAAGCCAG GAACATGGGC AGCACGATCC GGCAGCCCAA GCTGTCCAAT 
CTCTCGCCCT CCGTGATCGC CCAGACCAAC TGGAAGTTCG TGGAGGGCTT GCTGAAGGAG 
TGCCGCAATA AGACCAAGAG GATGCTAGTG GAAAAGATGG GCCGAGAGGC TGTGGAGCTG 
GGCCATGGAG AAGTCAACAT CACCGGTGTG GAGGAGAACA CTTTGATTGC TAGCCTTTGT 
GACCTCCTAG AAAGGATCTG GAGCCACGGG CTTCAGGTCA AACAGGGGAA GTCTGCCTTG 
TGGTCCCACT TGTTGCACTA TCAGGAAAAT CGGCAGAGGA AGCTGACGGC GGGAAGCTTG 
AGTACCTCAG GGATACTGCT GGACTCGGAG CGGAGGAAGA GTGACGCCAG CTCGGCCATG 
CCGCCCCTGA GGATCTCTCT CATCCAGGAC ATGAGGCACA TCCAGAACAT TGGGGAGATC 
AAAACGGACG TTGGCAAGGC CAGGGCCTGG GTCCGGCTGA GCATGGAGAA GAAGTTACTT 
TCCCGGCACC TGAAGCAGTT GCTCTCAGAT CACGAGCTCA CCAAGAAGCT GTACAAGCGC 
TATGCTTTCC TGCGCTGCGA CGACGAGAAG GAGCAGTTCC TCTATCACCT CCTGTCCTTC 
AACGCTGTGG ACTACTTCTG CTTTACCAAC GTCTTCACGA CAATCATGAT CCCGTACCAC 
ATCCTGATTG TCCCCAGCAA GAAGTTGGGC GGCTCCATGT TCACGGCCAA CCCTTGGATC 
TGCATCTCAG GAGAGCTGGG GGAGACGCAG ATCCTACAGG TCCCCAGGAA CGTGCTGGAG 
ATGACCTTTG AGTACCAGAA CCTGGGCAAG CTCACCACGG TCCAGATCGG CCATGATAAC 
TCGGGGCTGT ACGCCAAGTG GCTGGTGGAG CACGTGATGG TCAGGAACGA GATCACGGGA 
CACACATACA AGTTCCCCTG TGGCCGGTGG CTGGGGAAGG GCATGGACGA TGGCAGCCTG 
GAGAGGGTCC TGGTTGGGGA GCTGCTGACC TCTCACCCCG AAGCTGACGA GAGGCAGTGC 
CGAACGCCGC CCCTGCAGCA GTCTCCCAGC ATGATCCGGA GGCTGGTCAC CATCTCCCCC 
AGTAACAAGC CCAAGTTAAA CACGGGGCAG ATCCAGGAGT CCATTGGGGA GGCCGTCAAT 
GGCATTGTCA AGCACTTCCA TAAGCCAGAG AAAGAGCGTG GCAGCCTGAC GCTCCTGCTC 
TGTGGCGAGT GTGGCCTGGT ATCTGCCCTG GAGCAAGTTT TTCAGCATGG ATTTAAATCT 
CCCCGGCTCT TCAAGAACGT CTTCATCTGG GATTTTCTGG AAAAAGCACA AACCTACTAC 
GAGACCCTCG ACCAGAGTGA CCTCGTACCT GAGGAGAACT GGCAGACGAG GGCCCGAAAC 
TTCTGCCGCT TTGTCACCGC CATCAACAAC ACCCCCCGCA ACATCGGCAA AGATGGCAAG 
TTCCAGATGC TGGTGTGTCT GGGAGCCAGA GATCACCTCT TGCACCACTG GATTGCCCTG 
CTGGCCGACT GTCCGATCAC AGCACAGATG TATGAAGATG TGGCGCTCCT CAAGGACCAC 
ACGCTTGTGA ATTCCTTGAT CCGGGTCCTG CAGACTTTGC AGGAGTTCAA TATCACACTG 
GAGACGTCCC TGGTCAAAGG GATAGACATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031797855.1
CDS	16..3888
Translation	MSGGGGGGGGGSLPSRFADYFVICGLDTETGLEPDELSALCQYIRASKARDGSSPFIASTAEGENFEQTPLRRTFKSKVLARYPENVEWNPFDQDAVGMLCMPKGLAFKTQADPRSPQFHAFIITREDGSRTFGFALTFYEEVTSKQICSAMQTLYHMHNAEYDGLRAPAANGREGGAPQDGCDGAPGSKLQRFNSYDISRDTLYVSKCICLIAPMSFMKACRRALEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPPGRSLKFSGVYGPIVCQRPSTSELPLFDFPVREVFELLGVENVLQLFTCALLEFQILLYSQHYQRLMTVAETITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFVQEISEILMAFGIPPEGNLHCSESASKLKSLRACDIASDKRNGNIASSPLHTYGLLKENETIARLQALVQRTGVSLEKLDMREESSSNKDLKVQCEEEELRVYQLNIQIREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFVDNKIMCHDDDDKDPILRVFDTRVDKIRLLNVRTPTLRTSMYQKCTTVDEAEKAIELRLAKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQADVLSTGPASNKWTKRNAPAQWRRKDRQKQHTEHLRLDNDQREKYIQEARNMGSTIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVNITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQENRQRKLTAGSLSTSGILLDSERRKSDASSAMPPLRISLIQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETQILQVPRNVLEMTFEYQNLGKLTTVQIGHDNSGLYAKWLVEHVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGELLTSHPEADERQCRTPPLQQSPSMIRRLVTISPSNKPKLNTGQIQESIGEAVNGIVKHFHKPEKERGSLTLLLCGECGLVSALEQVFQHGFKSPRLFKNVFIWDFLEKAQTYYETLDQSDLVPEENWQTRARNFCRFVTAINNTPRNIGKDGKFQMLVCLGARDHLLHHWIALLADCPITAQMYEDVALLKDHTLVNSLIRVLQTLQEFNITLETSLVKGIDI

Target ORF information:

RefSeq Version	XM_031941995.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031941995.1

ORF Insert Sequence:

ATGAGCGGCG GCGGCGGCGG CGGCGGCGGG GGCTCGCTTC CCAGCCGCTT CGCCGACTAC 
TTTGTCATCT GCGGGCTGGA CACGGAGACG GGGCTGGAGC CGGACGAGCT GTCGGCTTTA 
TGCCAGTACA TACGGGCTTC TAAAGCCAGG GATGGCAGCA GCCCTTTCAT TGCCAGTACA 
GCAGAAGGAG AAAATTTTGA GCAGACACCC CTGAGACGGA CATTCAAATC TAAGGTTCTT 
GCCCGATACC CTGAGAATGT AGAATGGAAT CCTTTTGACC AAGATGCGGT GGGAATGTTG 
TGCATGCCCA AAGGGCTGGC GTTCAAGACG CAGGCTGATC CCAGGAGCCC CCAGTTCCAC 
GCCTTCATCA TCACGCGGGA GGACGGCTCC CGGACGTTCG GGTTTGCCCT CACCTTTTAC 
GAGGAGGTGA CCAGCAAGCA GATCTGCAGT GCGATGCAGA CGCTCTACCA CATGCACAAC 
GCCGAGTACG ACGGGCTCCG CGCGCCCGCT GCCAACGGCC GCGAGGGCGG CGCGCCCCAG 
GACGGCTGTG ACGGTGCGCC CGGCTCCAAG CTGCAGCGCT TCAACTCGTA CGACATCAGC 
CGGGACACGC TCTACGTCTC CAAGTGCATC TGCCTCATTG CGCCCATGTC TTTCATGAAG 
GCGTGCCGGC GGGCACTGGA GCAGCTCCAC CAGGCCGTGA CCTCCCCGCA GCCCCCGCCG 
CTGCCCCTCG AGAGCTACAT CTACAACATC CTCTACGAGG TGCCCCTGCC CCCGCCGGGC 
AGGTCCCTGA AGTTCTCCGG CGTCTACGGG CCCATCGTCT GCCAGAGGCC CAGCACCAGT 
GAGCTGCCGC TCTTCGACTT CCCCGTGCGC GAGGTCTTCG AGCTGCTTGG CGTGGAGAAT 
GTGCTCCAGC TCTTCACCTG CGCCCTGCTG GAGTTTCAGA TCCTGCTCTA CTCCCAGCAT 
TACCAGAGAC TGATGACTGT GGCCGAGACC ATCACAGCGC TCATGTTCCC TTTCCAGTGG 
CAGCACGTCT ACGTCCCCAT CCTCCCCGCC TCTCTCCTGC ACTTCCTAGA TGCTCCTGTT 
CCGTACCTGA TGGGCTTGCA CTCCAACGGC CTGGATGACC GCTCCAAACT AGAGTTGCCT 
CAAGAGGCCA ATCTCTGCTT TGTGGACATT GACAACCATT TCATTGAGCT GCCCGAGGAC 
CTGCCTCAGT TTCCCAACAA GCTAGAGTTT GTCCAGGAGA TCTCTGAGAT TCTTATGGCC 
TTTGGAATTC CCCCAGAAGG GAACCTGCAC TGCAGCGAGA GTGCCTCCAA ATTGAAGAGC 
CTCAGGGCCT GTGACATTGC CTCAGACAAA AGAAATGGGA ACATTGCCAG CTCCCCTCTG 
CACACCTATG GACTCCTCAA GGAGAATGAG ACCATTGCCA GGCTGCAAGC CCTGGTCCAA 
AGGACTGGGG TAAGCTTGGA AAAGCTGGAC ATGCGAGAAG AGAGCAGCAG CAATAAGGAT 
CTCAAAGTTC AGTGTGAGGA AGAGGAGCTC AGGGTTTACC AGCTGAACAT CCAGATCCGA 
GAAGTCTTTG CCAACCGCTT CACCCAGATG TTTGCCGACT ATGAAGTGTT TGTCATCCAG 
CCCAGCCAGG ATAAAGAGTC CTGGTTCACC AATAGGGAGC AGATGCAGAA CTTTGATAAG 
GCCTCTTTTT TATCAGATCA GCCTGAGCCC TACCTGCCAT TCCTCTCAAG GTTCCTGGAG 
ACCCAGATGT TTGCATCCTT TGTTGACAAT AAGATCATGT GCCACGATGA TGATGACAAA 
GACCCCATTC TGAGGGTGTT TGATACTCGA GTGGACAAGA TAAGGTTACT GAACGTGAGG 
ACGCCGACCC TGCGAACCTC AATGTACCAG AAGTGCACAA CTGTGGATGA AGCAGAGAAA 
GCGATTGAGC TGAGACTGGC AAAAATCGAC CACACTGCTA TTCACCCCCA CTTATTGGAC 
ATGAAGATTG GACAGGGGAA GTATGAGCCG GGATTCTTCC CCAAACTGCA GGCTGATGTG 
CTTTCAACCG GGCCAGCCAG CAACAAGTGG ACCAAGAGGA ATGCACCTGC CCAGTGGAGG 
CGGAAGGATA GACAGAAGCA GCACACAGAA CATCTACGCC TAGATAATGA CCAGAGGGAG 
AAGTACATCC AGGAAGCCAG GAACATGGGC AGCACGATCC GGCAGCCCAA GCTGTCCAAT 
CTCTCGCCCT CCGTGATCGC CCAGACCAAC TGGAAGTTCG TGGAGGGCTT GCTGAAGGAG 
TGCCGCAATA AGACCAAGAG GATGCTAGTG GAAAAGATGG GCCGAGAGGC TGTGGAGCTG 
GGCCATGGAG AAGTCAACAT CACCGGTGTG GAGGAGAACA CTTTGATTGC TAGCCTTTGT 
GACCTCCTAG AAAGGATCTG GAGCCACGGG CTTCAGGTCA AACAGGGGAA GTCTGCCTTG 
TGGTCCCACT TGTTGCACTA TCAGGAAAAT CGGCAGAGGA AGCTGACGGC GGGAAGCTTG 
AGTACCTCAG GGATACTGCT GGACTCGGAG CGGAGGAAGA GTGACGCCAG CTCGGCCATG 
CCGCCCCTGA GGATCTCTCT CATCCAGGAC ATGAGGCACA TCCAGAACAT TGGGGAGATC 
AAAACGGACG TTGGCAAGGC CAGGGCCTGG GTCCGGCTGA GCATGGAGAA GAAGTTACTT 
TCCCGGCACC TGAAGCAGTT GCTCTCAGAT CACGAGCTCA CCAAGAAGCT GTACAAGCGC 
TATGCTTTCC TGCGCTGCGA CGACGAGAAG GAGCAGTTCC TCTATCACCT CCTGTCCTTC 
AACGCTGTGG ACTACTTCTG CTTTACCAAC GTCTTCACGA CAATCATGAT CCCGTACCAC 
ATCCTGATTG TCCCCAGCAA GAAGTTGGGC GGCTCCATGT TCACGGCCAA CCCTTGGATC 
TGCATCTCAG GAGAGCTGGG GGAGACGCAG ATCCTACAGG TCCCCAGGAA CGTGCTGGAG 
ATGACCTTTG AGTACCAGAA CCTGGGCAAG CTCACCACGG TCCAGATCGG CCATGATAAC 
TCGGGGCTGT ACGCCAAGTG GCTGGTGGAG CACGTGATGG TCAGGAACGA GATCACGGGA 
CACACATACA AGTTCCCCTG TGGCCGGTGG CTGGGGAAGG GCATGGACGA TGGCAGCCTG 
GAGAGGGTCC TGGTTGGGGA GCTGCTGACC TCTCACCCCG AAGCTGACGA GAGGCAGTGC 
CGAACGCCGC CCCTGCAGCA GTCTCCCAGC ATGATCCGGA GGCTGGTCAC CATCTCCCCC 
AGTAACAAGC CCAAGTTAAA CACGGGGCAG ATCCAGGAGT CCATTGGGGA GGCCGTCAAT 
GGCATTGTCA AGCACTTCCA TAAGCCAGAG AAAGAGCGTG GCAGCCTGAC GCTCCTGCTC 
TGTGGCGAGT GTGGCCTGGT ATCTGCCCTG GAGCAAGTTT TTCAGCATGG ATTTAAATCT 
CCCCGGCTCT TCAAGAACGT CTTCATCTGG GATTTTCTGG AAAAAGCACA AACCTACTAC 
GAGACCCTCG ACCAGAGTGA CCTCGTACCT GAGGAGAACT GGCAGACGAG GGCCCGAAAC 
TTCTGCCGCT TTGTCACCGC CATCAACAAC ACCCCCCGCA ACATCGGCAA AGATGGCAAG 
TTCCAGATGC TGGTGTGTCT GGGAGCCAGA GATCACCTCT TGCACCACTG GATTGCCCTG 
CTGGCCGACT GTCCGATCAC AGCACAGATG TATGAAGATG TGGCGCTCCT CAAGGACCAC 
ACGCTTGTGA ATTCCTTGAT CCGGGTCCTG CAGACTTTGC AGGAGTTCAA TATCACACTG 
GAGACGTCCC TGGTCAAAGG GATAGACATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND5A ( XM_031941996.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031797856.1 Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa239248
Clone ID Related Accession (Same CDS sequence)	XM_031941996.1
Accession Version	XM_031941996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DENN domain-containing protein 5A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045431.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCGGCGGCGG CGGCGGCGGG GGCTCGCTTC CCAGCCGCTT CGCCGACTAC 
TTTGTCATCT GCGGGCTGGA CACGGAGACG GGGCTGGAGC CGGACGAGCT GTCGGCTTTA 
TGCCAGTACA TACGGGCTTC TAAAGCCAGG GATGGCAGCA GCCCTTTCAT TGCCAGTACA 
GCAGAAGGAG AAAATTTTGA GCAGACACCC CTGAGACGGA CATTCAAATC TAAGGTTCTT 
GCCCGATACC CTGAGAATGT AGAATGGAAT CCTTTTGACC AAGATGCGGT GGGAATGTTG 
TGCATGCCCA AAGGGCTGGC GTTCAAGACG CAGGCTGATC CCAGGAGCCC CCAGTTCCAC 
GCCTTCATCA TCACGCGGGA GGACGGCTCC CGGACGTTCG GGTTTGCCCT CACCTTTTAC 
GAGGAGGTGA CCAGCAAGCA GATCTGCAGT GCGATGCAGA CGCTCTACCA CATGCACAAC 
GCCGAGTACG ACGGGCTCCG CGCGCCCGCT GCCAACGGCC GCGAGGGCGG CGCGCCCCAG 
GACGGCTGTG ACGGTGCGCC CGGCTCCAAG CTGCAGCGCT TCAACTCGTA CGACATCAGC 
CGGGACACGC TCTACGTCTC CAAGTGCATC TGCCTCATTG CGCCCATGTC TTTCATGAAG 
GCGTGCCGGC GGGCACTGGA GCAGCTCCAC CAGGCCGTGA CCTCCCCGCA GCCCCCGCCG 
CTGCCCCTCG AGAGCTACAT CTACAACATC CTCTACGAGG TGCCCCTGCC CCCGCCGGGC 
AGGTCCCTGA AGTTCTCCGG CGTCTACGGG CCCATCGTCT GCCAGAGGCC CAGCACCAGT 
GAGCTGCCGC TCTTCGACTT CCCCGTGCGC GAGGTCTTCG AGCTGCTTGG CGTGGAGAAT 
GTGCTCCAGC TCTTCACCTG CGCCCTGCTG GAGTTTCAGA TCCTGCTCTA CTCCCAGCAT 
TACCAGAGAC TGATGACTGT GGCCGAGACC ATCACAGCGC TCATGTTCCC TTTCCAGTGG 
CAGCACGTCT ACGTCCCCAT CCTCCCCGCC TCTCTCCTGC ACTTCCTAGA TGCTCCTGTT 
CCGTACCTGA TGGGCTTGCA CTCCAACGGC CTGGATGACC GCTCCAAACT AGAGTTGCCT 
CAAGAGGCCA ATCTCTGCTT TGTGGACATT GACAACCATT TCATTGAGCT GCCCGAGGAC 
CTGCCTCAGT TTCCCAACAA GCTAGAGTTT GTCCAGGAGA TCTCTGAGAT TCTTATGGCC 
TTTGGAATTC CCCCAGAAGG GAACCTGCAC TGCAGCGAGA GTGCCTCCAA ATTGAAGAGC 
CTCAGGGCCT GTGACATTGC CTCAGACAAA AGAAATGGGA ACATTGCCAG CTCCCCTCTG 
CACACCTATG GACTCCTCAA GGAGAATGAG ACCATTGCCA GGCTGCAAGC CCTGGTCCAA 
AGGACTGGGC TGGACATGCG AGAAGAGAGC AGCAGCAATA AGGATCTCAA AGTTCAGTGT 
GAGGAAGAGG AGCTCAGGGT TTACCAGCTG AACATCCAGA TCCGAGAAGT CTTTGCCAAC 
CGCTTCACCC AGATGTTTGC CGACTATGAA GTGTTTGTCA TCCAGCCCAG CCAGGATAAA 
GAGTCCTGGT TCACCAATAG GGAGCAGATG CAGAACTTTG ATAAGGCCTC TTTTTTATCA 
GATCAGCCTG AGCCCTACCT GCCATTCCTC TCAAGGTTCC TGGAGACCCA GATGTTTGCA 
TCCTTTGTTG ACAATAAGAT CATGTGCCAC GATGATGATG ACAAAGACCC CATTCTGAGG 
GTGTTTGATA CTCGAGTGGA CAAGATAAGG TTACTGAACG TGAGGACGCC GACCCTGCGA 
ACCTCAATGT ACCAGAAGTG CACAACTGTG GATGAAGCAG AGAAAGCGAT TGAGCTGAGA 
CTGGCAAAAA TCGACCACAC TGCTATTCAC CCCCACTTAT TGGACATGAA GATTGGACAG 
GGGAAGTATG AGCCGGGATT CTTCCCCAAA CTGCAGGCTG ATGTGCTTTC AACCGGGCCA 
GCCAGCAACA AGTGGACCAA GAGGAATGCA CCTGCCCAGT GGAGGCGGAA GGATAGACAG 
AAGCAGCACA CAGAACATCT ACGCCTAGAT AATGACCAGA GGGAGAAGTA CATCCAGGAA 
GCCAGGAACA TGGGCAGCAC GATCCGGCAG CCCAAGCTGT CCAATCTCTC GCCCTCCGTG 
ATCGCCCAGA CCAACTGGAA GTTCGTGGAG GGCTTGCTGA AGGAGTGCCG CAATAAGACC 
AAGAGGATGC TAGTGGAAAA GATGGGCCGA GAGGCTGTGG AGCTGGGCCA TGGAGAAGTC 
AACATCACCG GTGTGGAGGA GAACACTTTG ATTGCTAGCC TTTGTGACCT CCTAGAAAGG 
ATCTGGAGCC ACGGGCTTCA GGTCAAACAG GGGAAGTCTG CCTTGTGGTC CCACTTGTTG 
CACTATCAGG AAAATCGGCA GAGGAAGCTG ACGGCGGGAA GCTTGAGTAC CTCAGGGATA 
CTGCTGGACT CGGAGCGGAG GAAGAGTGAC GCCAGCTCGG CCATGCCGCC CCTGAGGATC 
TCTCTCATCC AGGACATGAG GCACATCCAG AACATTGGGG AGATCAAAAC GGACGTTGGC 
AAGGCCAGGG CCTGGGTCCG GCTGAGCATG GAGAAGAAGT TACTTTCCCG GCACCTGAAG 
CAGTTGCTCT CAGATCACGA GCTCACCAAG AAGCTGTACA AGCGCTATGC TTTCCTGCGC 
TGCGACGACG AGAAGGAGCA GTTCCTCTAT CACCTCCTGT CCTTCAACGC TGTGGACTAC 
TTCTGCTTTA CCAACGTCTT CACGACAATC ATGATCCCGT ACCACATCCT GATTGTCCCC 
AGCAAGAAGT TGGGCGGCTC CATGTTCACG GCCAACCCTT GGATCTGCAT CTCAGGAGAG 
CTGGGGGAGA CGCAGATCCT ACAGGTCCCC AGGAACGTGC TGGAGATGAC CTTTGAGTAC 
CAGAACCTGG GCAAGCTCAC CACGGTCCAG ATCGGCCATG ATAACTCGGG GCTGTACGCC 
AAGTGGCTGG TGGAGCACGT GATGGTCAGG AACGAGATCA CGGGACACAC ATACAAGTTC 
CCCTGTGGCC GGTGGCTGGG GAAGGGCATG GACGATGGCA GCCTGGAGAG GGTCCTGGTT 
GGGGAGCTGC TGACCTCTCA CCCCGAAGCT GACGAGAGGC AGTGCCGAAC GCCGCCCCTG 
CAGCAGTCTC CCAGCATGAT CCGGAGGCTG GTCACCATCT CCCCCAGTAA CAAGCCCAAG 
TTAAACACGG GGCAGATCCA GGAGTCCATT GGGGAGGCCG TCAATGGCAT TGTCAAGCAC 
TTCCATAAGC CAGAGAAAGA GCGTGGCAGC CTGACGCTCC TGCTCTGTGG CGAGTGTGGC 
CTGGTATCTG CCCTGGAGCA AGTTTTTCAG CATGGATTTA AATCTCCCCG GCTCTTCAAG 
AACGTCTTCA TCTGGGATTT TCTGGAAAAA GCACAAACCT ACTACGAGAC CCTCGACCAG 
AGTGACCTCG TACCTGAGGA GAACTGGCAG ACGAGGGCCC GAAACTTCTG CCGCTTTGTC 
ACCGCCATCA ACAACACCCC CCGCAACATC GGCAAAGATG GCAAGTTCCA GATGCTGGTG 
TGTCTGGGAG CCAGAGATCA CCTCTTGCAC CACTGGATTG CCCTGCTGGC CGACTGTCCG 
ATCACAGCAC AGATGTATGA AGATGTGGCG CTCCTCAAGG ACCACACGCT TGTGAATTCC 
TTGATCCGGG TCCTGCAGAC TTTGCAGGAG TTCAATATCA CACTGGAGAC GTCCCTGGTC 
AAAGGGATAG ACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031797856.1
CDS	16..3873
Translation	MSGGGGGGGGGSLPSRFADYFVICGLDTETGLEPDELSALCQYIRASKARDGSSPFIASTAEGENFEQTPLRRTFKSKVLARYPENVEWNPFDQDAVGMLCMPKGLAFKTQADPRSPQFHAFIITREDGSRTFGFALTFYEEVTSKQICSAMQTLYHMHNAEYDGLRAPAANGREGGAPQDGCDGAPGSKLQRFNSYDISRDTLYVSKCICLIAPMSFMKACRRALEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPPGRSLKFSGVYGPIVCQRPSTSELPLFDFPVREVFELLGVENVLQLFTCALLEFQILLYSQHYQRLMTVAETITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFVQEISEILMAFGIPPEGNLHCSESASKLKSLRACDIASDKRNGNIASSPLHTYGLLKENETIARLQALVQRTGLDMREESSSNKDLKVQCEEEELRVYQLNIQIREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFVDNKIMCHDDDDKDPILRVFDTRVDKIRLLNVRTPTLRTSMYQKCTTVDEAEKAIELRLAKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQADVLSTGPASNKWTKRNAPAQWRRKDRQKQHTEHLRLDNDQREKYIQEARNMGSTIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVNITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQENRQRKLTAGSLSTSGILLDSERRKSDASSAMPPLRISLIQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETQILQVPRNVLEMTFEYQNLGKLTTVQIGHDNSGLYAKWLVEHVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGELLTSHPEADERQCRTPPLQQSPSMIRRLVTISPSNKPKLNTGQIQESIGEAVNGIVKHFHKPEKERGSLTLLLCGECGLVSALEQVFQHGFKSPRLFKNVFIWDFLEKAQTYYETLDQSDLVPEENWQTRARNFCRFVTAINNTPRNIGKDGKFQMLVCLGARDHLLHHWIALLADCPITAQMYEDVALLKDHTLVNSLIRVLQTLQEFNITLETSLVKGIDI

Target ORF information:

RefSeq Version	XM_031941996.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031941996.1

ORF Insert Sequence:

ATGAGCGGCG GCGGCGGCGG CGGCGGCGGG GGCTCGCTTC CCAGCCGCTT CGCCGACTAC 
TTTGTCATCT GCGGGCTGGA CACGGAGACG GGGCTGGAGC CGGACGAGCT GTCGGCTTTA 
TGCCAGTACA TACGGGCTTC TAAAGCCAGG GATGGCAGCA GCCCTTTCAT TGCCAGTACA 
GCAGAAGGAG AAAATTTTGA GCAGACACCC CTGAGACGGA CATTCAAATC TAAGGTTCTT 
GCCCGATACC CTGAGAATGT AGAATGGAAT CCTTTTGACC AAGATGCGGT GGGAATGTTG 
TGCATGCCCA AAGGGCTGGC GTTCAAGACG CAGGCTGATC CCAGGAGCCC CCAGTTCCAC 
GCCTTCATCA TCACGCGGGA GGACGGCTCC CGGACGTTCG GGTTTGCCCT CACCTTTTAC 
GAGGAGGTGA CCAGCAAGCA GATCTGCAGT GCGATGCAGA CGCTCTACCA CATGCACAAC 
GCCGAGTACG ACGGGCTCCG CGCGCCCGCT GCCAACGGCC GCGAGGGCGG CGCGCCCCAG 
GACGGCTGTG ACGGTGCGCC CGGCTCCAAG CTGCAGCGCT TCAACTCGTA CGACATCAGC 
CGGGACACGC TCTACGTCTC CAAGTGCATC TGCCTCATTG CGCCCATGTC TTTCATGAAG 
GCGTGCCGGC GGGCACTGGA GCAGCTCCAC CAGGCCGTGA CCTCCCCGCA GCCCCCGCCG 
CTGCCCCTCG AGAGCTACAT CTACAACATC CTCTACGAGG TGCCCCTGCC CCCGCCGGGC 
AGGTCCCTGA AGTTCTCCGG CGTCTACGGG CCCATCGTCT GCCAGAGGCC CAGCACCAGT 
GAGCTGCCGC TCTTCGACTT CCCCGTGCGC GAGGTCTTCG AGCTGCTTGG CGTGGAGAAT 
GTGCTCCAGC TCTTCACCTG CGCCCTGCTG GAGTTTCAGA TCCTGCTCTA CTCCCAGCAT 
TACCAGAGAC TGATGACTGT GGCCGAGACC ATCACAGCGC TCATGTTCCC TTTCCAGTGG 
CAGCACGTCT ACGTCCCCAT CCTCCCCGCC TCTCTCCTGC ACTTCCTAGA TGCTCCTGTT 
CCGTACCTGA TGGGCTTGCA CTCCAACGGC CTGGATGACC GCTCCAAACT AGAGTTGCCT 
CAAGAGGCCA ATCTCTGCTT TGTGGACATT GACAACCATT TCATTGAGCT GCCCGAGGAC 
CTGCCTCAGT TTCCCAACAA GCTAGAGTTT GTCCAGGAGA TCTCTGAGAT TCTTATGGCC 
TTTGGAATTC CCCCAGAAGG GAACCTGCAC TGCAGCGAGA GTGCCTCCAA ATTGAAGAGC 
CTCAGGGCCT GTGACATTGC CTCAGACAAA AGAAATGGGA ACATTGCCAG CTCCCCTCTG 
CACACCTATG GACTCCTCAA GGAGAATGAG ACCATTGCCA GGCTGCAAGC CCTGGTCCAA 
AGGACTGGGC TGGACATGCG AGAAGAGAGC AGCAGCAATA AGGATCTCAA AGTTCAGTGT 
GAGGAAGAGG AGCTCAGGGT TTACCAGCTG AACATCCAGA TCCGAGAAGT CTTTGCCAAC 
CGCTTCACCC AGATGTTTGC CGACTATGAA GTGTTTGTCA TCCAGCCCAG CCAGGATAAA 
GAGTCCTGGT TCACCAATAG GGAGCAGATG CAGAACTTTG ATAAGGCCTC TTTTTTATCA 
GATCAGCCTG AGCCCTACCT GCCATTCCTC TCAAGGTTCC TGGAGACCCA GATGTTTGCA 
TCCTTTGTTG ACAATAAGAT CATGTGCCAC GATGATGATG ACAAAGACCC CATTCTGAGG 
GTGTTTGATA CTCGAGTGGA CAAGATAAGG TTACTGAACG TGAGGACGCC GACCCTGCGA 
ACCTCAATGT ACCAGAAGTG CACAACTGTG GATGAAGCAG AGAAAGCGAT TGAGCTGAGA 
CTGGCAAAAA TCGACCACAC TGCTATTCAC CCCCACTTAT TGGACATGAA GATTGGACAG 
GGGAAGTATG AGCCGGGATT CTTCCCCAAA CTGCAGGCTG ATGTGCTTTC AACCGGGCCA 
GCCAGCAACA AGTGGACCAA GAGGAATGCA CCTGCCCAGT GGAGGCGGAA GGATAGACAG 
AAGCAGCACA CAGAACATCT ACGCCTAGAT AATGACCAGA GGGAGAAGTA CATCCAGGAA 
GCCAGGAACA TGGGCAGCAC GATCCGGCAG CCCAAGCTGT CCAATCTCTC GCCCTCCGTG 
ATCGCCCAGA CCAACTGGAA GTTCGTGGAG GGCTTGCTGA AGGAGTGCCG CAATAAGACC 
AAGAGGATGC TAGTGGAAAA GATGGGCCGA GAGGCTGTGG AGCTGGGCCA TGGAGAAGTC 
AACATCACCG GTGTGGAGGA GAACACTTTG ATTGCTAGCC TTTGTGACCT CCTAGAAAGG 
ATCTGGAGCC ACGGGCTTCA GGTCAAACAG GGGAAGTCTG CCTTGTGGTC CCACTTGTTG 
CACTATCAGG AAAATCGGCA GAGGAAGCTG ACGGCGGGAA GCTTGAGTAC CTCAGGGATA 
CTGCTGGACT CGGAGCGGAG GAAGAGTGAC GCCAGCTCGG CCATGCCGCC CCTGAGGATC 
TCTCTCATCC AGGACATGAG GCACATCCAG AACATTGGGG AGATCAAAAC GGACGTTGGC 
AAGGCCAGGG CCTGGGTCCG GCTGAGCATG GAGAAGAAGT TACTTTCCCG GCACCTGAAG 
CAGTTGCTCT CAGATCACGA GCTCACCAAG AAGCTGTACA AGCGCTATGC TTTCCTGCGC 
TGCGACGACG AGAAGGAGCA GTTCCTCTAT CACCTCCTGT CCTTCAACGC TGTGGACTAC 
TTCTGCTTTA CCAACGTCTT CACGACAATC ATGATCCCGT ACCACATCCT GATTGTCCCC 
AGCAAGAAGT TGGGCGGCTC CATGTTCACG GCCAACCCTT GGATCTGCAT CTCAGGAGAG 
CTGGGGGAGA CGCAGATCCT ACAGGTCCCC AGGAACGTGC TGGAGATGAC CTTTGAGTAC 
CAGAACCTGG GCAAGCTCAC CACGGTCCAG ATCGGCCATG ATAACTCGGG GCTGTACGCC 
AAGTGGCTGG TGGAGCACGT GATGGTCAGG AACGAGATCA CGGGACACAC ATACAAGTTC 
CCCTGTGGCC GGTGGCTGGG GAAGGGCATG GACGATGGCA GCCTGGAGAG GGTCCTGGTT 
GGGGAGCTGC TGACCTCTCA CCCCGAAGCT GACGAGAGGC AGTGCCGAAC GCCGCCCCTG 
CAGCAGTCTC CCAGCATGAT CCGGAGGCTG GTCACCATCT CCCCCAGTAA CAAGCCCAAG 
TTAAACACGG GGCAGATCCA GGAGTCCATT GGGGAGGCCG TCAATGGCAT TGTCAAGCAC 
TTCCATAAGC CAGAGAAAGA GCGTGGCAGC CTGACGCTCC TGCTCTGTGG CGAGTGTGGC 
CTGGTATCTG CCCTGGAGCA AGTTTTTCAG CATGGATTTA AATCTCCCCG GCTCTTCAAG 
AACGTCTTCA TCTGGGATTT TCTGGAAAAA GCACAAACCT ACTACGAGAC CCTCGACCAG 
AGTGACCTCG TACCTGAGGA GAACTGGCAG ACGAGGGCCC GAAACTTCTG CCGCTTTGTC 
ACCGCCATCA ACAACACCCC CCGCAACATC GGCAAAGATG GCAAGTTCCA GATGCTGGTG 
TGTCTGGGAG CCAGAGATCA CCTCTTGCAC CACTGGATTG CCCTGCTGGC CGACTGTCCG 
ATCACAGCAC AGATGTATGA AGATGTGGCG CTCCTCAAGG ACCACACGCT TGTGAATTCC 
TTGATCCGGG TCCTGCAGAC TTTGCAGGAG TTCAATATCA CACTGGAGAC GTCCCTGGTC 
AAAGGGATAG ACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND5A ( XM_031941997.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031797857.1 Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSa239249
Clone ID Related Accession (Same CDS sequence)	XM_031941997.1
Accession Version	XM_031941997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DENN domain-containing protein 5A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045431.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCG GCGGCGGCGG CGGCGGCGGG GGCTCGCTTC CCAGCCGCTT CGCCGACTAC 
TTTGTCATCT GCGGGCTGGA CACGGAGACG GGGCTGGAGC CGGACGAGCT GTCGGGAGAA 
AATTTTGAGC AGACACCCCT GAGACGGACA TTCAAATCTA AGGTTCTTGC CCGATACCCT 
GAGAATGTAG AATGGAATCC TTTTGACCAA GATGCGGTGG GAATGTTGTG CATGCCCAAA 
GGGCTGGCGT TCAAGACGCA GGCTGATCCC AGGAGCCCCC AGTTCCACGC CTTCATCATC 
ACGCGGGAGG ACGGCTCCCG GACGTTCGGG TTTGCCCTCA CCTTTTACGA GGAGGTGACC 
AGCAAGCAGA TCTGCAGTGC GATGCAGACG CTCTACCACA TGCACAACGC CGAGTACGAC 
GGGCTCCGCG CGCCCGCTGC CAACGGCCGC GAGGGCGGCG CGCCCCAGGA CGGCTGTGAC 
GGTGCGCCCG GCTCCAAGCT GCAGCGCTTC AACTCGTACG ACATCAGCCG GGACACGCTC 
TACGTCTCCA AGTGCATCTG CCTCATTGCG CCCATGTCTT TCATGAAGGC GTGCCGGCGG 
GCACTGGAGC AGCTCCACCA GGCCGTGACC TCCCCGCAGC CCCCGCCGCT GCCCCTCGAG 
AGCTACATCT ACAACATCCT CTACGAGGTG CCCCTGCCCC CGCCGGGCAG GTCCCTGAAG 
TTCTCCGGCG TCTACGGGCC CATCGTCTGC CAGAGGCCCA GCACCAGTGA GCTGCCGCTC 
TTCGACTTCC CCGTGCGCGA GGTCTTCGAG CTGCTTGGCG TGGAGAATGT GCTCCAGCTC 
TTCACCTGCG CCCTGCTGGA GTTTCAGATC CTGCTCTACT CCCAGCATTA CCAGAGACTG 
ATGACTGTGG CCGAGACCAT CACAGCGCTC ATGTTCCCTT TCCAGTGGCA GCACGTCTAC 
GTCCCCATCC TCCCCGCCTC TCTCCTGCAC TTCCTAGATG CTCCTGTTCC GTACCTGATG 
GGCTTGCACT CCAACGGCCT GGATGACCGC TCCAAACTAG AGTTGCCTCA AGAGGCCAAT 
CTCTGCTTTG TGGACATTGA CAACCATTTC ATTGAGCTGC CCGAGGACCT GCCTCAGTTT 
CCCAACAAGC TAGAGTTTGT CCAGGAGATC TCTGAGATTC TTATGGCCTT TGGAATTCCC 
CCAGAAGGGA ACCTGCACTG CAGCGAGAGT GCCTCCAAAT TGAAGAGCCT CAGGGCCTGT 
GACATTGCCT CAGACAAAAG AAATGGGAAC ATTGCCAGCT CCCCTCTGCA CACCTATGGA 
CTCCTCAAGG AGAATGAGAC CATTGCCAGG CTGCAAGCCC TGGTCCAAAG GACTGGGGTA 
AGCTTGGAAA AGCTGGACAT GCGAGAAGAG AGCAGCAGCA ATAAGGATCT CAAAGTTCAG 
TGTGAGGAAG AGGAGCTCAG GGTTTACCAG CTGAACATCC AGATCCGAGA AGTCTTTGCC 
AACCGCTTCA CCCAGATGTT TGCCGACTAT GAAGTGTTTG TCATCCAGCC CAGCCAGGAT 
AAAGAGTCCT GGTTCACCAA TAGGGAGCAG ATGCAGAACT TTGATAAGGC CTCTTTTTTA 
TCAGATCAGC CTGAGCCCTA CCTGCCATTC CTCTCAAGGT TCCTGGAGAC CCAGATGTTT 
GCATCCTTTG TTGACAATAA GATCATGTGC CACGATGATG ATGACAAAGA CCCCATTCTG 
AGGGTGTTTG ATACTCGAGT GGACAAGATA AGGTTACTGA ACGTGAGGAC GCCGACCCTG 
CGAACCTCAA TGTACCAGAA GTGCACAACT GTGGATGAAG CAGAGAAAGC GATTGAGCTG 
AGACTGGCAA AAATCGACCA CACTGCTATT CACCCCCACT TATTGGACAT GAAGATTGGA 
CAGGGGAAGT ATGAGCCGGG ATTCTTCCCC AAACTGCAGG CTGATGTGCT TTCAACCGGG 
CCAGCCAGCA ACAAGTGGAC CAAGAGGAAT GCACCTGCCC AGTGGAGGCG GAAGGATAGA 
CAGAAGCAGC ACACAGAACA TCTACGCCTA GATAATGACC AGAGGGAGAA GTACATCCAG 
GAAGCCAGGA ACATGGGCAG CACGATCCGG CAGCCCAAGC TGTCCAATCT CTCGCCCTCC 
GTGATCGCCC AGACCAACTG GAAGTTCGTG GAGGGCTTGC TGAAGGAGTG CCGCAATAAG 
ACCAAGAGGA TGCTAGTGGA AAAGATGGGC CGAGAGGCTG TGGAGCTGGG CCATGGAGAA 
GTCAACATCA CCGGTGTGGA GGAGAACACT TTGATTGCTA GCCTTTGTGA CCTCCTAGAA 
AGGATCTGGA GCCACGGGCT TCAGGTCAAA CAGGGGAAGT CTGCCTTGTG GTCCCACTTG 
TTGCACTATC AGGAAAATCG GCAGAGGAAG CTGACGGCGG GAAGCTTGAG TACCTCAGGG 
ATACTGCTGG ACTCGGAGCG GAGGAAGAGT GACGCCAGCT CGGCCATGCC GCCCCTGAGG 
ATCTCTCTCA TCCAGGACAT GAGGCACATC CAGAACATTG GGGAGATCAA AACGGACGTT 
GGCAAGGCCA GGGCCTGGGT CCGGCTGAGC ATGGAGAAGA AGTTACTTTC CCGGCACCTG 
AAGCAGTTGC TCTCAGATCA CGAGCTCACC AAGAAGCTGT ACAAGCGCTA TGCTTTCCTG 
CGCTGCGACG ACGAGAAGGA GCAGTTCCTC TATCACCTCC TGTCCTTCAA CGCTGTGGAC 
TACTTCTGCT TTACCAACGT CTTCACGACA ATCATGATCC CGTACCACAT CCTGATTGTC 
CCCAGCAAGA AGTTGGGCGG CTCCATGTTC ACGGCCAACC CTTGGATCTG CATCTCAGGA 
GAGCTGGGGG AGACGCAGAT CCTACAGGTC CCCAGGAACG TGCTGGAGAT GACCTTTGAG 
TACCAGAACC TGGGCAAGCT CACCACGGTC CAGATCGGCC ATGATAACTC GGGGCTGTAC 
GCCAAGTGGC TGGTGGAGCA CGTGATGGTC AGGAACGAGA TCACGGGACA CACATACAAG 
TTCCCCTGTG GCCGGTGGCT GGGGAAGGGC ATGGACGATG GCAGCCTGGA GAGGGTCCTG 
GTTGGGGAGC TGCTGACCTC TCACCCCGAA GCTGACGAGA GGCAGTGCCG AACGCCGCCC 
CTGCAGCAGT CTCCCAGCAT GATCCGGAGG CTGGTCACCA TCTCCCCCAG TAACAAGCCC 
AAGTTAAACA CGGGGCAGAT CCAGGAGTCC ATTGGGGAGG CCGTCAATGG CATTGTCAAG 
CACTTCCATA AGCCAGAGAA AGAGCGTGGC AGCCTGACGC TCCTGCTCTG TGGCGAGTGT 
GGCCTGGTAT CTGCCCTGGA GCAAGTTTTT CAGCATGGAT TTAAATCTCC CCGGCTCTTC 
AAGAACGTCT TCATCTGGGA TTTTCTGGAA AAAGCACAAA CCTACTACGA GACCCTCGAC 
CAGAGTGACC TCGTACCTGA GGAGAACTGG CAGACGAGGG CCCGAAACTT CTGCCGCTTT 
GTCACCGCCA TCAACAACAC CCCCCGCAAC ATCGGCAAAG ATGGCAAGTT CCAGATGCTG 
GTGTGTCTGG GAGCCAGAGA TCACCTCTTG CACCACTGGA TTGCCCTGCT GGCCGACTGT 
CCGATCACAG CACAGATGTA TGAAGATGTG GCGCTCCTCA AGGACCACAC GCTTGTGAAT 
TCCTTGATCC GGGTCCTGCA GACTTTGCAG GAGTTCAATA TCACACTGGA GACGTCCCTG 
GTCAAAGGGA TAGACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031797857.1
CDS	16..3816
Translation	MSGGGGGGGGGSLPSRFADYFVICGLDTETGLEPDELSGENFEQTPLRRTFKSKVLARYPENVEWNPFDQDAVGMLCMPKGLAFKTQADPRSPQFHAFIITREDGSRTFGFALTFYEEVTSKQICSAMQTLYHMHNAEYDGLRAPAANGREGGAPQDGCDGAPGSKLQRFNSYDISRDTLYVSKCICLIAPMSFMKACRRALEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPPGRSLKFSGVYGPIVCQRPSTSELPLFDFPVREVFELLGVENVLQLFTCALLEFQILLYSQHYQRLMTVAETITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFVQEISEILMAFGIPPEGNLHCSESASKLKSLRACDIASDKRNGNIASSPLHTYGLLKENETIARLQALVQRTGVSLEKLDMREESSSNKDLKVQCEEEELRVYQLNIQIREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFVDNKIMCHDDDDKDPILRVFDTRVDKIRLLNVRTPTLRTSMYQKCTTVDEAEKAIELRLAKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQADVLSTGPASNKWTKRNAPAQWRRKDRQKQHTEHLRLDNDQREKYIQEARNMGSTIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVNITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQENRQRKLTAGSLSTSGILLDSERRKSDASSAMPPLRISLIQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETQILQVPRNVLEMTFEYQNLGKLTTVQIGHDNSGLYAKWLVEHVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGELLTSHPEADERQCRTPPLQQSPSMIRRLVTISPSNKPKLNTGQIQESIGEAVNGIVKHFHKPEKERGSLTLLLCGECGLVSALEQVFQHGFKSPRLFKNVFIWDFLEKAQTYYETLDQSDLVPEENWQTRARNFCRFVTAINNTPRNIGKDGKFQMLVCLGARDHLLHHWIALLADCPITAQMYEDVALLKDHTLVNSLIRVLQTLQEFNITLETSLVKGIDI

Target ORF information:

RefSeq Version	XM_031941997.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031941997.1

ORF Insert Sequence:

ATGAGCGGCG GCGGCGGCGG CGGCGGCGGG GGCTCGCTTC CCAGCCGCTT CGCCGACTAC 
TTTGTCATCT GCGGGCTGGA CACGGAGACG GGGCTGGAGC CGGACGAGCT GTCGGGAGAA 
AATTTTGAGC AGACACCCCT GAGACGGACA TTCAAATCTA AGGTTCTTGC CCGATACCCT 
GAGAATGTAG AATGGAATCC TTTTGACCAA GATGCGGTGG GAATGTTGTG CATGCCCAAA 
GGGCTGGCGT TCAAGACGCA GGCTGATCCC AGGAGCCCCC AGTTCCACGC CTTCATCATC 
ACGCGGGAGG ACGGCTCCCG GACGTTCGGG TTTGCCCTCA CCTTTTACGA GGAGGTGACC 
AGCAAGCAGA TCTGCAGTGC GATGCAGACG CTCTACCACA TGCACAACGC CGAGTACGAC 
GGGCTCCGCG CGCCCGCTGC CAACGGCCGC GAGGGCGGCG CGCCCCAGGA CGGCTGTGAC 
GGTGCGCCCG GCTCCAAGCT GCAGCGCTTC AACTCGTACG ACATCAGCCG GGACACGCTC 
TACGTCTCCA AGTGCATCTG CCTCATTGCG CCCATGTCTT TCATGAAGGC GTGCCGGCGG 
GCACTGGAGC AGCTCCACCA GGCCGTGACC TCCCCGCAGC CCCCGCCGCT GCCCCTCGAG 
AGCTACATCT ACAACATCCT CTACGAGGTG CCCCTGCCCC CGCCGGGCAG GTCCCTGAAG 
TTCTCCGGCG TCTACGGGCC CATCGTCTGC CAGAGGCCCA GCACCAGTGA GCTGCCGCTC 
TTCGACTTCC CCGTGCGCGA GGTCTTCGAG CTGCTTGGCG TGGAGAATGT GCTCCAGCTC 
TTCACCTGCG CCCTGCTGGA GTTTCAGATC CTGCTCTACT CCCAGCATTA CCAGAGACTG 
ATGACTGTGG CCGAGACCAT CACAGCGCTC ATGTTCCCTT TCCAGTGGCA GCACGTCTAC 
GTCCCCATCC TCCCCGCCTC TCTCCTGCAC TTCCTAGATG CTCCTGTTCC GTACCTGATG 
GGCTTGCACT CCAACGGCCT GGATGACCGC TCCAAACTAG AGTTGCCTCA AGAGGCCAAT 
CTCTGCTTTG TGGACATTGA CAACCATTTC ATTGAGCTGC CCGAGGACCT GCCTCAGTTT 
CCCAACAAGC TAGAGTTTGT CCAGGAGATC TCTGAGATTC TTATGGCCTT TGGAATTCCC 
CCAGAAGGGA ACCTGCACTG CAGCGAGAGT GCCTCCAAAT TGAAGAGCCT CAGGGCCTGT 
GACATTGCCT CAGACAAAAG AAATGGGAAC ATTGCCAGCT CCCCTCTGCA CACCTATGGA 
CTCCTCAAGG AGAATGAGAC CATTGCCAGG CTGCAAGCCC TGGTCCAAAG GACTGGGGTA 
AGCTTGGAAA AGCTGGACAT GCGAGAAGAG AGCAGCAGCA ATAAGGATCT CAAAGTTCAG 
TGTGAGGAAG AGGAGCTCAG GGTTTACCAG CTGAACATCC AGATCCGAGA AGTCTTTGCC 
AACCGCTTCA CCCAGATGTT TGCCGACTAT GAAGTGTTTG TCATCCAGCC CAGCCAGGAT 
AAAGAGTCCT GGTTCACCAA TAGGGAGCAG ATGCAGAACT TTGATAAGGC CTCTTTTTTA 
TCAGATCAGC CTGAGCCCTA CCTGCCATTC CTCTCAAGGT TCCTGGAGAC CCAGATGTTT 
GCATCCTTTG TTGACAATAA GATCATGTGC CACGATGATG ATGACAAAGA CCCCATTCTG 
AGGGTGTTTG ATACTCGAGT GGACAAGATA AGGTTACTGA ACGTGAGGAC GCCGACCCTG 
CGAACCTCAA TGTACCAGAA GTGCACAACT GTGGATGAAG CAGAGAAAGC GATTGAGCTG 
AGACTGGCAA AAATCGACCA CACTGCTATT CACCCCCACT TATTGGACAT GAAGATTGGA 
CAGGGGAAGT ATGAGCCGGG ATTCTTCCCC AAACTGCAGG CTGATGTGCT TTCAACCGGG 
CCAGCCAGCA ACAAGTGGAC CAAGAGGAAT GCACCTGCCC AGTGGAGGCG GAAGGATAGA 
CAGAAGCAGC ACACAGAACA TCTACGCCTA GATAATGACC AGAGGGAGAA GTACATCCAG 
GAAGCCAGGA ACATGGGCAG CACGATCCGG CAGCCCAAGC TGTCCAATCT CTCGCCCTCC 
GTGATCGCCC AGACCAACTG GAAGTTCGTG GAGGGCTTGC TGAAGGAGTG CCGCAATAAG 
ACCAAGAGGA TGCTAGTGGA AAAGATGGGC CGAGAGGCTG TGGAGCTGGG CCATGGAGAA 
GTCAACATCA CCGGTGTGGA GGAGAACACT TTGATTGCTA GCCTTTGTGA CCTCCTAGAA 
AGGATCTGGA GCCACGGGCT TCAGGTCAAA CAGGGGAAGT CTGCCTTGTG GTCCCACTTG 
TTGCACTATC AGGAAAATCG GCAGAGGAAG CTGACGGCGG GAAGCTTGAG TACCTCAGGG 
ATACTGCTGG ACTCGGAGCG GAGGAAGAGT GACGCCAGCT CGGCCATGCC GCCCCTGAGG 
ATCTCTCTCA TCCAGGACAT GAGGCACATC CAGAACATTG GGGAGATCAA AACGGACGTT 
GGCAAGGCCA GGGCCTGGGT CCGGCTGAGC ATGGAGAAGA AGTTACTTTC CCGGCACCTG 
AAGCAGTTGC TCTCAGATCA CGAGCTCACC AAGAAGCTGT ACAAGCGCTA TGCTTTCCTG 
CGCTGCGACG ACGAGAAGGA GCAGTTCCTC TATCACCTCC TGTCCTTCAA CGCTGTGGAC 
TACTTCTGCT TTACCAACGT CTTCACGACA ATCATGATCC CGTACCACAT CCTGATTGTC 
CCCAGCAAGA AGTTGGGCGG CTCCATGTTC ACGGCCAACC CTTGGATCTG CATCTCAGGA 
GAGCTGGGGG AGACGCAGAT CCTACAGGTC CCCAGGAACG TGCTGGAGAT GACCTTTGAG 
TACCAGAACC TGGGCAAGCT CACCACGGTC CAGATCGGCC ATGATAACTC GGGGCTGTAC 
GCCAAGTGGC TGGTGGAGCA CGTGATGGTC AGGAACGAGA TCACGGGACA CACATACAAG 
TTCCCCTGTG GCCGGTGGCT GGGGAAGGGC ATGGACGATG GCAGCCTGGA GAGGGTCCTG 
GTTGGGGAGC TGCTGACCTC TCACCCCGAA GCTGACGAGA GGCAGTGCCG AACGCCGCCC 
CTGCAGCAGT CTCCCAGCAT GATCCGGAGG CTGGTCACCA TCTCCCCCAG TAACAAGCCC 
AAGTTAAACA CGGGGCAGAT CCAGGAGTCC ATTGGGGAGG CCGTCAATGG CATTGTCAAG 
CACTTCCATA AGCCAGAGAA AGAGCGTGGC AGCCTGACGC TCCTGCTCTG TGGCGAGTGT 
GGCCTGGTAT CTGCCCTGGA GCAAGTTTTT CAGCATGGAT TTAAATCTCC CCGGCTCTTC 
AAGAACGTCT TCATCTGGGA TTTTCTGGAA AAAGCACAAA CCTACTACGA GACCCTCGAC 
CAGAGTGACC TCGTACCTGA GGAGAACTGG CAGACGAGGG CCCGAAACTT CTGCCGCTTT 
GTCACCGCCA TCAACAACAC CCCCCGCAAC ATCGGCAAAG ATGGCAAGTT CCAGATGCTG 
GTGTGTCTGG GAGCCAGAGA TCACCTCTTG CACCACTGGA TTGCCCTGCT GGCCGACTGT 
CCGATCACAG CACAGATGTA TGAAGATGTG GCGCTCCTCA AGGACCACAC GCTTGTGAAT 
TCCTTGATCC GGGTCCTGCA GACTTTGCAG GAGTTCAATA TCACACTGGA GACGTCCCTG 
GTCAAAGGGA TAGACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND5A ( XM_031941998.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031797858.1 Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X4, mRNA.

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CloneID	OSa239250
Clone ID Related Accession (Same CDS sequence)	XM_031941998.1
Accession Version	XM_031941998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	DENN domain-containing protein 5A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045431.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCA TGCCCAAAGG GCTGGCGTTC AAGACGCAGG CTGATCCCAG GAGCCCCCAG 
TTCCACGCCT TCATCATCAC GCGGGAGGAC GGCTCCCGGA CGTTCGGGTT TGCCCTCACC 
TTTTACGAGG AGGTGACCAG CAAGCAGATC TGCAGTGCGA TGCAGACGCT CTACCACATG 
CACAACGCCG AGTACGACGG GCTCCGCGCG CCCGCTGCCA ACGGCCGCGA GGGCGGCGCG 
CCCCAGGACG GCTGTGACGG TGCGCCCGGC TCCAAGCTGC AGCGCTTCAA CTCGTACGAC 
ATCAGCCGGG ACACGCTCTA CGTCTCCAAG TGCATCTGCC TCATTGCGCC CATGTCTTTC 
ATGAAGGCGT GCCGGCGGGC ACTGGAGCAG CTCCACCAGG CCGTGACCTC CCCGCAGCCC 
CCGCCGCTGC CCCTCGAGAG CTACATCTAC AACATCCTCT ACGAGGTGCC CCTGCCCCCG 
CCGGGCAGGT CCCTGAAGTT CTCCGGCGTC TACGGGCCCA TCGTCTGCCA GAGGCCCAGC 
ACCAGTGAGC TGCCGCTCTT CGACTTCCCC GTGCGCGAGG TCTTCGAGCT GCTTGGCGTG 
GAGAATGTGC TCCAGCTCTT CACCTGCGCC CTGCTGGAGT TTCAGATCCT GCTCTACTCC 
CAGCATTACC AGAGACTGAT GACTGTGGCC GAGACCATCA CAGCGCTCAT GTTCCCTTTC 
CAGTGGCAGC ACGTCTACGT CCCCATCCTC CCCGCCTCTC TCCTGCACTT CCTAGATGCT 
CCTGTTCCGT ACCTGATGGG CTTGCACTCC AACGGCCTGG ATGACCGCTC CAAACTAGAG 
TTGCCTCAAG AGGCCAATCT CTGCTTTGTG GACATTGACA ACCATTTCAT TGAGCTGCCC 
GAGGACCTGC CTCAGTTTCC CAACAAGCTA GAGTTTGTCC AGGAGATCTC TGAGATTCTT 
ATGGCCTTTG GAATTCCCCC AGAAGGGAAC CTGCACTGCA GCGAGAGTGC CTCCAAATTG 
AAGAGCCTCA GGGCCTGTGA CATTGCCTCA GACAAAAGAA ATGGGAACAT TGCCAGCTCC 
CCTCTGCACA CCTATGGACT CCTCAAGGAG AATGAGACCA TTGCCAGGCT GCAAGCCCTG 
GTCCAAAGGA CTGGGGTAAG CTTGGAAAAG CTGGACATGC GAGAAGAGAG CAGCAGCAAT 
AAGGATCTCA AAGTTCAGTG TGAGGAAGAG GAGCTCAGGG TTTACCAGCT GAACATCCAG 
ATCCGAGAAG TCTTTGCCAA CCGCTTCACC CAGATGTTTG CCGACTATGA AGTGTTTGTC 
ATCCAGCCCA GCCAGGATAA AGAGTCCTGG TTCACCAATA GGGAGCAGAT GCAGAACTTT 
GATAAGGCCT CTTTTTTATC AGATCAGCCT GAGCCCTACC TGCCATTCCT CTCAAGGTTC 
CTGGAGACCC AGATGTTTGC ATCCTTTGTT GACAATAAGA TCATGTGCCA CGATGATGAT 
GACAAAGACC CCATTCTGAG GGTGTTTGAT ACTCGAGTGG ACAAGATAAG GTTACTGAAC 
GTGAGGACGC CGACCCTGCG AACCTCAATG TACCAGAAGT GCACAACTGT GGATGAAGCA 
GAGAAAGCGA TTGAGCTGAG ACTGGCAAAA ATCGACCACA CTGCTATTCA CCCCCACTTA 
TTGGACATGA AGATTGGACA GGGGAAGTAT GAGCCGGGAT TCTTCCCCAA ACTGCAGGCT 
GATGTGCTTT CAACCGGGCC AGCCAGCAAC AAGTGGACCA AGAGGAATGC ACCTGCCCAG 
TGGAGGCGGA AGGATAGACA GAAGCAGCAC ACAGAACATC TACGCCTAGA TAATGACCAG 
AGGGAGAAGT ACATCCAGGA AGCCAGGAAC ATGGGCAGCA CGATCCGGCA GCCCAAGCTG 
TCCAATCTCT CGCCCTCCGT GATCGCCCAG ACCAACTGGA AGTTCGTGGA GGGCTTGCTG 
AAGGAGTGCC GCAATAAGAC CAAGAGGATG CTAGTGGAAA AGATGGGCCG AGAGGCTGTG 
GAGCTGGGCC ATGGAGAAGT CAACATCACC GGTGTGGAGG AGAACACTTT GATTGCTAGC 
CTTTGTGACC TCCTAGAAAG GATCTGGAGC CACGGGCTTC AGGTCAAACA GGGGAAGTCT 
GCCTTGTGGT CCCACTTGTT GCACTATCAG GAAAATCGGC AGAGGAAGCT GACGGCGGGA 
AGCTTGAGTA CCTCAGGGAT ACTGCTGGAC TCGGAGCGGA GGAAGAGTGA CGCCAGCTCG 
GCCATGCCGC CCCTGAGGAT CTCTCTCATC CAGGACATGA GGCACATCCA GAACATTGGG 
GAGATCAAAA CGGACGTTGG CAAGGCCAGG GCCTGGGTCC GGCTGAGCAT GGAGAAGAAG 
TTACTTTCCC GGCACCTGAA GCAGTTGCTC TCAGATCACG AGCTCACCAA GAAGCTGTAC 
AAGCGCTATG CTTTCCTGCG CTGCGACGAC GAGAAGGAGC AGTTCCTCTA TCACCTCCTG 
TCCTTCAACG CTGTGGACTA CTTCTGCTTT ACCAACGTCT TCACGACAAT CATGATCCCG 
TACCACATCC TGATTGTCCC CAGCAAGAAG TTGGGCGGCT CCATGTTCAC GGCCAACCCT 
TGGATCTGCA TCTCAGGAGA GCTGGGGGAG ACGCAGATCC TACAGGTCCC CAGGAACGTG 
CTGGAGATGA CCTTTGAGTA CCAGAACCTG GGCAAGCTCA CCACGGTCCA GATCGGCCAT 
GATAACTCGG GGCTGTACGC CAAGTGGCTG GTGGAGCACG TGATGGTCAG GAACGAGATC 
ACGGGACACA CATACAAGTT CCCCTGTGGC CGGTGGCTGG GGAAGGGCAT GGACGATGGC 
AGCCTGGAGA GGGTCCTGGT TGGGGAGCTG CTGACCTCTC ACCCCGAAGC TGACGAGAGG 
CAGTGCCGAA CGCCGCCCCT GCAGCAGTCT CCCAGCATGA TCCGGAGGCT GGTCACCATC 
TCCCCCAGTA ACAAGCCCAA GTTAAACACG GGGCAGATCC AGGAGTCCAT TGGGGAGGCC 
GTCAATGGCA TTGTCAAGCA CTTCCATAAG CCAGAGAAAG AGCGTGGCAG CCTGACGCTC 
CTGCTCTGTG GCGAGTGTGG CCTGGTATCT GCCCTGGAGC AAGTTTTTCA GCATGGATTT 
AAATCTCCCC GGCTCTTCAA GAACGTCTTC ATCTGGGATT TTCTGGAAAA AGCACAAACC 
TACTACGAGA CCCTCGACCA GAGTGACCTC GTACCTGAGG AGAACTGGCA GACGAGGGCC 
CGAAACTTCT GCCGCTTTGT CACCGCCATC AACAACACCC CCCGCAACAT CGGCAAAGAT 
GGCAAGTTCC AGATGCTGGT GTGTCTGGGA GCCAGAGATC ACCTCTTGCA CCACTGGATT 
GCCCTGCTGG CCGACTGTCC GATCACAGCA CAGATGTATG AAGATGTGGC GCTCCTCAAG 
GACCACACGC TTGTGAATTC CTTGATCCGG GTCCTGCAGA CTTTGCAGGA GTTCAATATC 
ACACTGGAGA CGTCCCTGGT CAAAGGGATA GACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031797858.1
CDS	494..4072
Translation	MLCMPKGLAFKTQADPRSPQFHAFIITREDGSRTFGFALTFYEEVTSKQICSAMQTLYHMHNAEYDGLRAPAANGREGGAPQDGCDGAPGSKLQRFNSYDISRDTLYVSKCICLIAPMSFMKACRRALEQLHQAVTSPQPPPLPLESYIYNILYEVPLPPPGRSLKFSGVYGPIVCQRPSTSELPLFDFPVREVFELLGVENVLQLFTCALLEFQILLYSQHYQRLMTVAETITALMFPFQWQHVYVPILPASLLHFLDAPVPYLMGLHSNGLDDRSKLELPQEANLCFVDIDNHFIELPEDLPQFPNKLEFVQEISEILMAFGIPPEGNLHCSESASKLKSLRACDIASDKRNGNIASSPLHTYGLLKENETIARLQALVQRTGVSLEKLDMREESSSNKDLKVQCEEEELRVYQLNIQIREVFANRFTQMFADYEVFVIQPSQDKESWFTNREQMQNFDKASFLSDQPEPYLPFLSRFLETQMFASFVDNKIMCHDDDDKDPILRVFDTRVDKIRLLNVRTPTLRTSMYQKCTTVDEAEKAIELRLAKIDHTAIHPHLLDMKIGQGKYEPGFFPKLQADVLSTGPASNKWTKRNAPAQWRRKDRQKQHTEHLRLDNDQREKYIQEARNMGSTIRQPKLSNLSPSVIAQTNWKFVEGLLKECRNKTKRMLVEKMGREAVELGHGEVNITGVEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQENRQRKLTAGSLSTSGILLDSERRKSDASSAMPPLRISLIQDMRHIQNIGEIKTDVGKARAWVRLSMEKKLLSRHLKQLLSDHELTKKLYKRYAFLRCDDEKEQFLYHLLSFNAVDYFCFTNVFTTIMIPYHILIVPSKKLGGSMFTANPWICISGELGETQILQVPRNVLEMTFEYQNLGKLTTVQIGHDNSGLYAKWLVEHVMVRNEITGHTYKFPCGRWLGKGMDDGSLERVLVGELLTSHPEADERQCRTPPLQQSPSMIRRLVTISPSNKPKLNTGQIQESIGEAVNGIVKHFHKPEKERGSLTLLLCGECGLVSALEQVFQHGFKSPRLFKNVFIWDFLEKAQTYYETLDQSDLVPEENWQTRARNFCRFVTAINNTPRNIGKDGKFQMLVCLGARDHLLHHWIALLADCPITAQMYEDVALLKDHTLVNSLIRVLQTLQEFNITLETSLVKGIDI

Target ORF information:

RefSeq Version	XM_031941998.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii DENN domain containing 5A (DENND5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031941998.1

ORF Insert Sequence:

ATGTTGTGCA TGCCCAAAGG GCTGGCGTTC AAGACGCAGG CTGATCCCAG GAGCCCCCAG 
TTCCACGCCT TCATCATCAC GCGGGAGGAC GGCTCCCGGA CGTTCGGGTT TGCCCTCACC 
TTTTACGAGG AGGTGACCAG CAAGCAGATC TGCAGTGCGA TGCAGACGCT CTACCACATG 
CACAACGCCG AGTACGACGG GCTCCGCGCG CCCGCTGCCA ACGGCCGCGA GGGCGGCGCG 
CCCCAGGACG GCTGTGACGG TGCGCCCGGC TCCAAGCTGC AGCGCTTCAA CTCGTACGAC 
ATCAGCCGGG ACACGCTCTA CGTCTCCAAG TGCATCTGCC TCATTGCGCC CATGTCTTTC 
ATGAAGGCGT GCCGGCGGGC ACTGGAGCAG CTCCACCAGG CCGTGACCTC CCCGCAGCCC 
CCGCCGCTGC CCCTCGAGAG CTACATCTAC AACATCCTCT ACGAGGTGCC CCTGCCCCCG 
CCGGGCAGGT CCCTGAAGTT CTCCGGCGTC TACGGGCCCA TCGTCTGCCA GAGGCCCAGC 
ACCAGTGAGC TGCCGCTCTT CGACTTCCCC GTGCGCGAGG TCTTCGAGCT GCTTGGCGTG 
GAGAATGTGC TCCAGCTCTT CACCTGCGCC CTGCTGGAGT TTCAGATCCT GCTCTACTCC 
CAGCATTACC AGAGACTGAT GACTGTGGCC GAGACCATCA CAGCGCTCAT GTTCCCTTTC 
CAGTGGCAGC ACGTCTACGT CCCCATCCTC CCCGCCTCTC TCCTGCACTT CCTAGATGCT 
CCTGTTCCGT ACCTGATGGG CTTGCACTCC AACGGCCTGG ATGACCGCTC CAAACTAGAG 
TTGCCTCAAG AGGCCAATCT CTGCTTTGTG GACATTGACA ACCATTTCAT TGAGCTGCCC 
GAGGACCTGC CTCAGTTTCC CAACAAGCTA GAGTTTGTCC AGGAGATCTC TGAGATTCTT 
ATGGCCTTTG GAATTCCCCC AGAAGGGAAC CTGCACTGCA GCGAGAGTGC CTCCAAATTG 
AAGAGCCTCA GGGCCTGTGA CATTGCCTCA GACAAAAGAA ATGGGAACAT TGCCAGCTCC 
CCTCTGCACA CCTATGGACT CCTCAAGGAG AATGAGACCA TTGCCAGGCT GCAAGCCCTG 
GTCCAAAGGA CTGGGGTAAG CTTGGAAAAG CTGGACATGC GAGAAGAGAG CAGCAGCAAT 
AAGGATCTCA AAGTTCAGTG TGAGGAAGAG GAGCTCAGGG TTTACCAGCT GAACATCCAG 
ATCCGAGAAG TCTTTGCCAA CCGCTTCACC CAGATGTTTG CCGACTATGA AGTGTTTGTC 
ATCCAGCCCA GCCAGGATAA AGAGTCCTGG TTCACCAATA GGGAGCAGAT GCAGAACTTT 
GATAAGGCCT CTTTTTTATC AGATCAGCCT GAGCCCTACC TGCCATTCCT CTCAAGGTTC 
CTGGAGACCC AGATGTTTGC ATCCTTTGTT GACAATAAGA TCATGTGCCA CGATGATGAT 
GACAAAGACC CCATTCTGAG GGTGTTTGAT ACTCGAGTGG ACAAGATAAG GTTACTGAAC 
GTGAGGACGC CGACCCTGCG AACCTCAATG TACCAGAAGT GCACAACTGT GGATGAAGCA 
GAGAAAGCGA TTGAGCTGAG ACTGGCAAAA ATCGACCACA CTGCTATTCA CCCCCACTTA 
TTGGACATGA AGATTGGACA GGGGAAGTAT GAGCCGGGAT TCTTCCCCAA ACTGCAGGCT 
GATGTGCTTT CAACCGGGCC AGCCAGCAAC AAGTGGACCA AGAGGAATGC ACCTGCCCAG 
TGGAGGCGGA AGGATAGACA GAAGCAGCAC ACAGAACATC TACGCCTAGA TAATGACCAG 
AGGGAGAAGT ACATCCAGGA AGCCAGGAAC ATGGGCAGCA CGATCCGGCA GCCCAAGCTG 
TCCAATCTCT CGCCCTCCGT GATCGCCCAG ACCAACTGGA AGTTCGTGGA GGGCTTGCTG 
AAGGAGTGCC GCAATAAGAC CAAGAGGATG CTAGTGGAAA AGATGGGCCG AGAGGCTGTG 
GAGCTGGGCC ATGGAGAAGT CAACATCACC GGTGTGGAGG AGAACACTTT GATTGCTAGC 
CTTTGTGACC TCCTAGAAAG GATCTGGAGC CACGGGCTTC AGGTCAAACA GGGGAAGTCT 
GCCTTGTGGT CCCACTTGTT GCACTATCAG GAAAATCGGC AGAGGAAGCT GACGGCGGGA 
AGCTTGAGTA CCTCAGGGAT ACTGCTGGAC TCGGAGCGGA GGAAGAGTGA CGCCAGCTCG 
GCCATGCCGC CCCTGAGGAT CTCTCTCATC CAGGACATGA GGCACATCCA GAACATTGGG 
GAGATCAAAA CGGACGTTGG CAAGGCCAGG GCCTGGGTCC GGCTGAGCAT GGAGAAGAAG 
TTACTTTCCC GGCACCTGAA GCAGTTGCTC TCAGATCACG AGCTCACCAA GAAGCTGTAC 
AAGCGCTATG CTTTCCTGCG CTGCGACGAC GAGAAGGAGC AGTTCCTCTA TCACCTCCTG 
TCCTTCAACG CTGTGGACTA CTTCTGCTTT ACCAACGTCT TCACGACAAT CATGATCCCG 
TACCACATCC TGATTGTCCC CAGCAAGAAG TTGGGCGGCT CCATGTTCAC GGCCAACCCT 
TGGATCTGCA TCTCAGGAGA GCTGGGGGAG ACGCAGATCC TACAGGTCCC CAGGAACGTG 
CTGGAGATGA CCTTTGAGTA CCAGAACCTG GGCAAGCTCA CCACGGTCCA GATCGGCCAT 
GATAACTCGG GGCTGTACGC CAAGTGGCTG GTGGAGCACG TGATGGTCAG GAACGAGATC 
ACGGGACACA CATACAAGTT CCCCTGTGGC CGGTGGCTGG GGAAGGGCAT GGACGATGGC 
AGCCTGGAGA GGGTCCTGGT TGGGGAGCTG CTGACCTCTC ACCCCGAAGC TGACGAGAGG 
CAGTGCCGAA CGCCGCCCCT GCAGCAGTCT CCCAGCATGA TCCGGAGGCT GGTCACCATC 
TCCCCCAGTA ACAAGCCCAA GTTAAACACG GGGCAGATCC AGGAGTCCAT TGGGGAGGCC 
GTCAATGGCA TTGTCAAGCA CTTCCATAAG CCAGAGAAAG AGCGTGGCAG CCTGACGCTC 
CTGCTCTGTG GCGAGTGTGG CCTGGTATCT GCCCTGGAGC AAGTTTTTCA GCATGGATTT 
AAATCTCCCC GGCTCTTCAA GAACGTCTTC ATCTGGGATT TTCTGGAAAA AGCACAAACC 
TACTACGAGA CCCTCGACCA GAGTGACCTC GTACCTGAGG AGAACTGGCA GACGAGGGCC 
CGAAACTTCT GCCGCTTTGT CACCGCCATC AACAACACCC CCCGCAACAT CGGCAAAGAT 
GGCAAGTTCC AGATGCTGGT GTGTCTGGGA GCCAGAGATC ACCTCTTGCA CCACTGGATT 
GCCCTGCTGG CCGACTGTCC GATCACAGCA CAGATGTATG AAGATGTGGC GCTCCTCAAG 
GACCACACGC TTGTGAATTC CTTGATCCGG GTCCTGCAGA CTTTGCAGGA GTTCAATATC 
ACACTGGAGA CGTCCCTGGT CAAAGGGATA GACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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DENND5A cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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