Recql4 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Recql4
Entrez Gene ID	101843183
Full Name	RecQ like helicase 4
Gene Type	protein-coding
Organism	Mesocricetus auratus(golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005086006.2	XP_005086063.1	ATP-dependent DNA helicase Q4
XM_005086006.3	XP_005086063.1	ATP-dependent DNA helicase Q4

Pathways from BioSystems
Gene Ontology

The following Recql4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Recql4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe140554	XM_005086006.3 Latest version!	Mesocricetus auratus RecQ like helicase 4 (Recql4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
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OMe140554	XM_005086006.2	Mesocricetus auratus RecQ protein-like 4 (Recql4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Recql4 ( XM_005086006.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005086063.1 Mesocricetus auratus RecQ protein-like 4 (Recql4), mRNA.

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CloneID	OMe140554
Clone ID Related Accession (Same CDS sequence)	XM_005086006.2 , XM_005086006.3
Accession Version	XM_005086006.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	ATP-dependent DNA helicase Q4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801815.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 13, 2015 this sequence version replaced XM_005086006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAC TGGCGAGCGT TCGCGCGCGG CTGCAGGAGT GGGAACGCGC TTTCGCGCGG 
CTGCACGGGA GACGGCCAGC GCGGGAGGAC GTGGAGGCGG CCCCTGAGGA CACGCGCGCG 
CTCTACCGTG AGTACCGCAA CCTGAAGCAT GCAGCGCGCC AGGCCGGCGA CGGACACCGC 
AGCCTGGAGC AGTCTCTCGC CGAGACAGCT GAGGAGGCAC AGGAACCAAG CTGCTGGGGT 
CCCCACCTGA ATCGAGCCGC AACCCAGAAT ACACAGCCTA CGCCCAGGCA GGGCCCACTG 
GGTTCAGTAC AGGACTATGG GAAGAGGCTC AAAGCCAATC TGGAAAGCAA CCCGAAAACA 
GCAAAGCTGC AGACTAGGCC AGCCCTGAGC CGAAAATGCC AGCCTCGGAA GAGAGCCTTG 
TCCACCGTGC CCACCCCAAG GCCCCCAGGC CCAAGGACCT GTCCAGACGA AACTAATGAT 
GTACTTCCCA GGGTTGCTGA GCCCCAGCCA CGGCTAGGCC ATCTCCAGCA GCTTCAGGCA 
TCCCTGAGTC TGAGGCTGAG TTCCCTAGAT CCTGGCTGGT TAGAGCGATG TCAAGACAGA 
GTTTCAGACC TTCTAGAGGT CCCCAGTGCC TGTGGGCTTG ACCTGGATGG AAAGGAGTCA 
CAGCCCCAGA TATCAGGCAA GGTGAATGTC CTTGATCCTG GTACCCGTTC AGAAGGGTCT 
TCACAGAGCC CAGGGGGCCT AGCCACACAG CCACAGAGCC CCAAACCCAG CAACAAGACA 
GACAGGAAAT GGACGCGGAA TGAGAGTTCG GAGGACCGTG CACAAGCCCA GGACAGTGGC 
CAAGCAGGAC TGCTGTCTGA GGGAGCCCGG GCTAGCGCAC ATGAGAAAGA CCCTCCAGGA 
GAAGCTGCGA GCGCGCCCCG GCCCCACAAC TCCTCCAAGA GGGCCAGGAC AGAGACGGCC 
AAGGACAAAG CCCACGCCTC CACTAGACCC GCTTTCCTGG ACAGAGGGAA CTACATCCGA 
CTCAACATGA AACACAAGTG CTATGTACGA GTTGGCGCCA GTCGAGGCAG GCTTCTCCGG 
AAGCAGGTGT GGAGGCAGAA GTGGCGGAAG AAAGCGGGGC AGTTTGGGGG CGGCAGAGCC 
GGAGCCGAAG CCCAGGACGC CTGTTTCCGG TGTGGGCAGT GTGGTCACTG GGCTTCCCAG 
TGCCCCCAGG CAGGCTCCAC CCGGACCTTC CAGGAAGACA GTGGCAGGGA TGACAAACGG 
CCCGTTCCCA CTTTGGAAGA AGAAGCACAG ATGGCAGGCA CTCCTTCCTG TCACCTGCCT 
GGTCAGGACA CAGACCCTGA TGGGCCAAAG CTGCAAGTCC CCTGTCCTCC CCGCCCAGTG 
CCGCCCCTCT ACCCCCCGGG GCCCTTGGGG CATACAACAG AGACCCCTGC TGAGGTATTC 
CAGGCCCTGC AGCAGCTGGG GCACCAAGCG TTCCGCCCTG GACAAGAACA GGCAATCATG 
CGGATTCTCT CAGGCATCTC CACTCTGTTA GTGCTGCCCA CAGGCGCTGG CAAGTCTCTG 
TGCTACCAGC TGCCAGCACT GCTGTATGCC CAGCGAAGCC CCTGCCTCAC CCTGGTGGTC 
TCCCCTCTCC TGTCACTCAT GGATGACCAG GTGTCTGACC TGCCCGCATG TCTGAAGGCA 
GCCTGCCTCC ACTCGGGTAT GACCAAAAAA CAACGAGAAG CTGTCTTGAA AAAGGTAAAG 
GCGGCCCAGG TGCATGTGCT GCTTGTGTCC CCAGAGGCCT TGGTCGGGTG TGGGGCTAAG 
GGCCCTGCCA GCCTCCCCCA GGCCTCTGAA CTGCCTCCAA TCGCCTTCGC CTGCATCGAC 
GAGGTCCACT GCCTCTCCCA GTGGTCACAC AACTTCCGGC CCTGCTACCT GCGTGTTTGC 
AAAGTACTTC GGGAGCATCT GGGTGTGCAC TGCCTTTTGG GCCTCACAGC CACAGCCACA 
CGAAGCACTG CCCGTGACGT GGCCCAGCAC CTGGGCGTAG CCGAAGAGCT GGAGCTCAGT 
GGGTTAGCCA GCATCCCTGC TAATCTGCAC CTCTCCGTGT CCATGGACAG AGACTCAGAC 
CAGGCTCTGG TGACACTGCT GCAAGGTGAC CGCTTTTGTG CCCTGGACTC TGTCATCATT 
TACTGTACTC GACGAAAAGA TACAGAACGG GTGGCCGCCC TCCTCCGGAC CCGCCTGTCT 
ATGGCGACAG AGCCAAGGCC TAGAGGTTGT GGCCCTGAGG CTCTAGCTGA AGCATACCAT 
GCTGGCATGT GCAGCCAGGA ACGGCGACGC GTGCAACAGG CCTTCATGCA GGGCCACCTG 
CGGGTGGTGG TGGCCACGGT AGCCTTTGGG ATGGGGCTGG ACCGGCCAGA TGTCCGGGCC 
GTGCTGCACC TGGGACTGCC TCCAAACTTC GAGAGCTATG TGCAAGCTAT TGGCCGTGCA 
GGACGTGATG GGAAGCCTGC CCACTGTCAC CTGTTCCTGC AGCCCCAGGG TGAAGACCTT 
TGGGAGCTGC GCAGACATGC CCATGCCGAT GGCACTGACT TCTTGGCTGT GAAGAAGCTG 
GTGCAACGTG TATTTCCATC CTGCACATGC ACCCAGAGGC CTATGTCCAA ACCCCTGCTT 
GAGGAGACCA AAGAGCGCGA CAGCCAACAA ACACACCCTG CATTGCGACA GGCCTGCCCG 
GGCCACACGC GGGCATTCCC AGTGCAGTCT ACAGTACAGG CTCTGGACAT GACAGAGGAG 
GCTATTGAGA CTCTGCTGTG CTATCTGGAA CTCCACCCTC GGCGCTGGCT GAAGCTGCTG 
TCATGCACTT ATGCCCACTG TCACCTACGC TGCCCTGGGG GCAGTGCTCA GCTGCAAGCT 
CTGGCCCACA GGTGTCCCCC ATTGGCTGCA TGTTTGGCCA AGTACCCACC TAAAGACACG 
AGTCGGGGCC GCAGCTCCTT AGACTTTGAC ATGGTGGAGC TGGCAGACTC GATGGGCTGG 
GAGCTGGCTT CTGTACGGCG GGCTCTCCAC CAGCTGCGGT GGGACCAGGA GCCAAAGAAA 
GGTGCACCAC GGGGCACAGG GGTGCTGGTG GAGTTCAGCG AGTTGGCCTT TCATGTGCAC 
AGTCCTGGTG ACCTGACAGA TGAGGAGAAG GACCAGATCT GCGACTTCCT GTACAGCCGT 
GTACAGACTC GTGAACACAA GGCCCTGGCC CATCTACACC AAATATCTAA GGCCTTTCGC 
AGTGTGGCCT TTCCCAGCTG CGGACCCTGT CTAGAGCAGG CTGATGAGGA TCGCAGCAAC 
CAGTTAAAGA CCTTGCTCAG CCACTACTTT GAGGAAGAGG AAGAGGAAGC TGTGAAGGAT 
GAGCAGGGTC CAGAGCCTGG GCAGAGCGAG CTTCAGGACT GGGAGGACCA TATTCGCCGT 
GATATCCACC AGCTCCTGTC CCTGAGGCCA GAGGAGAGGT TTTCAGGACG GGCTGTGGCC 
CGCATCTTCC ATGGCATCGC AAGTCCCTGC TACCCAGCCC AGATATATGG GAAGGACCAG 
CGCTTCTGGA GAAAGTATCT GCATGTGAAC TTCCATGCCG TGATGCGCCT GGCCACAGAA 
GAACTGCTGA GAGGCCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005086063.1
CDS	1..3621
Translation	MERLASVRARLQEWERAFARLHGRRPAREDVEAAPEDTRALYREYRNLKHAARQAGDGHRSLEQSLAETAEEAQEPSCWGPHLNRAATQNTQPTPRQGPLGSVQDYGKRLKANLESNPKTAKLQTRPALSRKCQPRKRALSTVPTPRPPGPRTCPDETNDVLPRVAEPQPRLGHLQQLQASLSLRLSSLDPGWLERCQDRVSDLLEVPSACGLDLDGKESQPQISGKVNVLDPGTRSEGSSQSPGGLATQPQSPKPSNKTDRKWTRNESSEDRAQAQDSGQAGLLSEGARASAHEKDPPGEAASAPRPHNSSKRARTETAKDKAHASTRPAFLDRGNYIRLNMKHKCYVRVGASRGRLLRKQVWRQKWRKKAGQFGGGRAGAEAQDACFRCGQCGHWASQCPQAGSTRTFQEDSGRDDKRPVPTLEEEAQMAGTPSCHLPGQDTDPDGPKLQVPCPPRPVPPLYPPGPLGHTTETPAEVFQALQQLGHQAFRPGQEQAIMRILSGISTLLVLPTGAGKSLCYQLPALLYAQRSPCLTLVVSPLLSLMDDQVSDLPACLKAACLHSGMTKKQREAVLKKVKAAQVHVLLVSPEALVGCGAKGPASLPQASELPPIAFACIDEVHCLSQWSHNFRPCYLRVCKVLREHLGVHCLLGLTATATRSTARDVAQHLGVAEELELSGLASIPANLHLSVSMDRDSDQALVTLLQGDRFCALDSVIIYCTRRKDTERVAALLRTRLSMATEPRPRGCGPEALAEAYHAGMCSQERRRVQQAFMQGHLRVVVATVAFGMGLDRPDVRAVLHLGLPPNFESYVQAIGRAGRDGKPAHCHLFLQPQGEDLWELRRHAHADGTDFLAVKKLVQRVFPSCTCTQRPMSKPLLEETKERDSQQTHPALRQACPGHTRAFPVQSTVQALDMTEEAIETLLCYLELHPRRWLKLLSCTYAHCHLRCPGGSAQLQALAHRCPPLAACLAKYPPKDTSRGRSSLDFDMVELADSMGWELASVRRALHQLRWDQEPKKGAPRGTGVLVEFSELAFHVHSPGDLTDEEKDQICDFLYSRVQTREHKALAHLHQISKAFRSVAFPSCGPCLEQADEDRSNQLKTLLSHYFEEEEEEAVKDEQGPEPGQSELQDWEDHIRRDIHQLLSLRPEERFSGRAVARIFHGIASPCYPAQIYGKDQRFWRKYLHVNFHAVMRLATEELLRGR

Target ORF information:

RefSeq Version	XM_005086006.2
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus RecQ protein-like 4 (Recql4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005086006.2

ORF Insert Sequence:

ATGGAAAGAC TGGCGAGCGT TCGCGCGCGG CTGCAGGAGT GGGAACGCGC TTTCGCGCGG 
CTGCACGGGA GACGGCCAGC GCGGGAGGAC GTGGAGGCGG CCCCTGAGGA CACGCGCGCG 
CTCTACCGTG AGTACCGCAA CCTGAAGCAT GCAGCGCGCC AGGCCGGCGA CGGACACCGC 
AGCCTGGAGC AGTCTCTCGC CGAGACAGCT GAGGAGGCAC AGGAACCAAG CTGCTGGGGT 
CCCCACCTGA ATCGAGCCGC AACCCAGAAT ACACAGCCTA CGCCCAGGCA GGGCCCACTG 
GGTTCAGTAC AGGACTATGG GAAGAGGCTC AAAGCCAATC TGGAAAGCAA CCCGAAAACA 
GCAAAGCTGC AGACTAGGCC AGCCCTGAGC CGAAAATGCC AGCCTCGGAA GAGAGCCTTG 
TCCACCGTGC CCACCCCAAG GCCCCCAGGC CCAAGGACCT GTCCAGACGA AACTAATGAT 
GTACTTCCCA GGGTTGCTGA GCCCCAGCCA CGGCTAGGCC ATCTCCAGCA GCTTCAGGCA 
TCCCTGAGTC TGAGGCTGAG TTCCCTAGAT CCTGGCTGGT TAGAGCGATG TCAAGACAGA 
GTTTCAGACC TTCTAGAGGT CCCCAGTGCC TGTGGGCTTG ACCTGGATGG AAAGGAGTCA 
CAGCCCCAGA TATCAGGCAA GGTGAATGTC CTTGATCCTG GTACCCGTTC AGAAGGGTCT 
TCACAGAGCC CAGGGGGCCT AGCCACACAG CCACAGAGCC CCAAACCCAG CAACAAGACA 
GACAGGAAAT GGACGCGGAA TGAGAGTTCG GAGGACCGTG CACAAGCCCA GGACAGTGGC 
CAAGCAGGAC TGCTGTCTGA GGGAGCCCGG GCTAGCGCAC ATGAGAAAGA CCCTCCAGGA 
GAAGCTGCGA GCGCGCCCCG GCCCCACAAC TCCTCCAAGA GGGCCAGGAC AGAGACGGCC 
AAGGACAAAG CCCACGCCTC CACTAGACCC GCTTTCCTGG ACAGAGGGAA CTACATCCGA 
CTCAACATGA AACACAAGTG CTATGTACGA GTTGGCGCCA GTCGAGGCAG GCTTCTCCGG 
AAGCAGGTGT GGAGGCAGAA GTGGCGGAAG AAAGCGGGGC AGTTTGGGGG CGGCAGAGCC 
GGAGCCGAAG CCCAGGACGC CTGTTTCCGG TGTGGGCAGT GTGGTCACTG GGCTTCCCAG 
TGCCCCCAGG CAGGCTCCAC CCGGACCTTC CAGGAAGACA GTGGCAGGGA TGACAAACGG 
CCCGTTCCCA CTTTGGAAGA AGAAGCACAG ATGGCAGGCA CTCCTTCCTG TCACCTGCCT 
GGTCAGGACA CAGACCCTGA TGGGCCAAAG CTGCAAGTCC CCTGTCCTCC CCGCCCAGTG 
CCGCCCCTCT ACCCCCCGGG GCCCTTGGGG CATACAACAG AGACCCCTGC TGAGGTATTC 
CAGGCCCTGC AGCAGCTGGG GCACCAAGCG TTCCGCCCTG GACAAGAACA GGCAATCATG 
CGGATTCTCT CAGGCATCTC CACTCTGTTA GTGCTGCCCA CAGGCGCTGG CAAGTCTCTG 
TGCTACCAGC TGCCAGCACT GCTGTATGCC CAGCGAAGCC CCTGCCTCAC CCTGGTGGTC 
TCCCCTCTCC TGTCACTCAT GGATGACCAG GTGTCTGACC TGCCCGCATG TCTGAAGGCA 
GCCTGCCTCC ACTCGGGTAT GACCAAAAAA CAACGAGAAG CTGTCTTGAA AAAGGTAAAG 
GCGGCCCAGG TGCATGTGCT GCTTGTGTCC CCAGAGGCCT TGGTCGGGTG TGGGGCTAAG 
GGCCCTGCCA GCCTCCCCCA GGCCTCTGAA CTGCCTCCAA TCGCCTTCGC CTGCATCGAC 
GAGGTCCACT GCCTCTCCCA GTGGTCACAC AACTTCCGGC CCTGCTACCT GCGTGTTTGC 
AAAGTACTTC GGGAGCATCT GGGTGTGCAC TGCCTTTTGG GCCTCACAGC CACAGCCACA 
CGAAGCACTG CCCGTGACGT GGCCCAGCAC CTGGGCGTAG CCGAAGAGCT GGAGCTCAGT 
GGGTTAGCCA GCATCCCTGC TAATCTGCAC CTCTCCGTGT CCATGGACAG AGACTCAGAC 
CAGGCTCTGG TGACACTGCT GCAAGGTGAC CGCTTTTGTG CCCTGGACTC TGTCATCATT 
TACTGTACTC GACGAAAAGA TACAGAACGG GTGGCCGCCC TCCTCCGGAC CCGCCTGTCT 
ATGGCGACAG AGCCAAGGCC TAGAGGTTGT GGCCCTGAGG CTCTAGCTGA AGCATACCAT 
GCTGGCATGT GCAGCCAGGA ACGGCGACGC GTGCAACAGG CCTTCATGCA GGGCCACCTG 
CGGGTGGTGG TGGCCACGGT AGCCTTTGGG ATGGGGCTGG ACCGGCCAGA TGTCCGGGCC 
GTGCTGCACC TGGGACTGCC TCCAAACTTC GAGAGCTATG TGCAAGCTAT TGGCCGTGCA 
GGACGTGATG GGAAGCCTGC CCACTGTCAC CTGTTCCTGC AGCCCCAGGG TGAAGACCTT 
TGGGAGCTGC GCAGACATGC CCATGCCGAT GGCACTGACT TCTTGGCTGT GAAGAAGCTG 
GTGCAACGTG TATTTCCATC CTGCACATGC ACCCAGAGGC CTATGTCCAA ACCCCTGCTT 
GAGGAGACCA AAGAGCGCGA CAGCCAACAA ACACACCCTG CATTGCGACA GGCCTGCCCG 
GGCCACACGC GGGCATTCCC AGTGCAGTCT ACAGTACAGG CTCTGGACAT GACAGAGGAG 
GCTATTGAGA CTCTGCTGTG CTATCTGGAA CTCCACCCTC GGCGCTGGCT GAAGCTGCTG 
TCATGCACTT ATGCCCACTG TCACCTACGC TGCCCTGGGG GCAGTGCTCA GCTGCAAGCT 
CTGGCCCACA GGTGTCCCCC ATTGGCTGCA TGTTTGGCCA AGTACCCACC TAAAGACACG 
AGTCGGGGCC GCAGCTCCTT AGACTTTGAC ATGGTGGAGC TGGCAGACTC GATGGGCTGG 
GAGCTGGCTT CTGTACGGCG GGCTCTCCAC CAGCTGCGGT GGGACCAGGA GCCAAAGAAA 
GGTGCACCAC GGGGCACAGG GGTGCTGGTG GAGTTCAGCG AGTTGGCCTT TCATGTGCAC 
AGTCCTGGTG ACCTGACAGA TGAGGAGAAG GACCAGATCT GCGACTTCCT GTACAGCCGT 
GTACAGACTC GTGAACACAA GGCCCTGGCC CATCTACACC AAATATCTAA GGCCTTTCGC 
AGTGTGGCCT TTCCCAGCTG CGGACCCTGT CTAGAGCAGG CTGATGAGGA TCGCAGCAAC 
CAGTTAAAGA CCTTGCTCAG CCACTACTTT GAGGAAGAGG AAGAGGAAGC TGTGAAGGAT 
GAGCAGGGTC CAGAGCCTGG GCAGAGCGAG CTTCAGGACT GGGAGGACCA TATTCGCCGT 
GATATCCACC AGCTCCTGTC CCTGAGGCCA GAGGAGAGGT TTTCAGGACG GGCTGTGGCC 
CGCATCTTCC ATGGCATCGC AAGTCCCTGC TACCCAGCCC AGATATATGG GAAGGACCAG 
CGCTTCTGGA GAAAGTATCT GCATGTGAAC TTCCATGCCG TGATGCGCCT GGCCACAGAA 
GAACTGCTGA GAGGCCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Recql4 ( XM_005086006.3 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005086063.1 Mesocricetus auratus RecQ like helicase 4 (Recql4), mRNA.

Price & Availability↑

CloneID	OMe140554
Clone ID Related Accession (Same CDS sequence)	XM_005086006.2 , XM_005086006.3
Accession Version	XM_005086006.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	ATP-dependent DNA helicase Q4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801815.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_005086006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAC TGGCGAGCGT TCGCGCGCGG CTGCAGGAGT GGGAACGCGC TTTCGCGCGG 
CTGCACGGGA GACGGCCAGC GCGGGAGGAC GTGGAGGCGG CCCCTGAGGA CACGCGCGCG 
CTCTACCGTG AGTACCGCAA CCTGAAGCAT GCAGCGCGCC AGGCCGGCGA CGGACACCGC 
AGCCTGGAGC AGTCTCTCGC CGAGACAGCT GAGGAGGCAC AGGAACCAAG CTGCTGGGGT 
CCCCACCTGA ATCGAGCCGC AACCCAGAAT ACACAGCCTA CGCCCAGGCA GGGCCCACTG 
GGTTCAGTAC AGGACTATGG GAAGAGGCTC AAAGCCAATC TGGAAAGCAA CCCGAAAACA 
GCAAAGCTGC AGACTAGGCC AGCCCTGAGC CGAAAATGCC AGCCTCGGAA GAGAGCCTTG 
TCCACCGTGC CCACCCCAAG GCCCCCAGGC CCAAGGACCT GTCCAGACGA AACTAATGAT 
GTACTTCCCA GGGTTGCTGA GCCCCAGCCA CGGCTAGGCC ATCTCCAGCA GCTTCAGGCA 
TCCCTGAGTC TGAGGCTGAG TTCCCTAGAT CCTGGCTGGT TAGAGCGATG TCAAGACAGA 
GTTTCAGACC TTCTAGAGGT CCCCAGTGCC TGTGGGCTTG ACCTGGATGG AAAGGAGTCA 
CAGCCCCAGA TATCAGGCAA GGTGAATGTC CTTGATCCTG GTACCCGTTC AGAAGGGTCT 
TCACAGAGCC CAGGGGGCCT AGCCACACAG CCACAGAGCC CCAAACCCAG CAACAAGACA 
GACAGGAAAT GGACGCGGAA TGAGAGTTCG GAGGACCGTG CACAAGCCCA GGACAGTGGC 
CAAGCAGGAC TGCTGTCTGA GGGAGCCCGG GCTAGCGCAC ATGAGAAAGA CCCTCCAGGA 
GAAGCTGCGA GCGCGCCCCG GCCCCACAAC TCCTCCAAGA GGGCCAGGAC AGAGACGGCC 
AAGGACAAAG CCCACGCCTC CACTAGACCC GCTTTCCTGG ACAGAGGGAA CTACATCCGA 
CTCAACATGA AACACAAGTG CTATGTACGA GTTGGCGCCA GTCGAGGCAG GCTTCTCCGG 
AAGCAGGTGT GGAGGCAGAA GTGGCGGAAG AAAGCGGGGC AGTTTGGGGG CGGCAGAGCC 
GGAGCCGAAG CCCAGGACGC CTGTTTCCGG TGTGGGCAGT GTGGTCACTG GGCTTCCCAG 
TGCCCCCAGG CAGGCTCCAC CCGGACCTTC CAGGAAGACA GTGGCAGGGA TGACAAACGG 
CCCGTTCCCA CTTTGGAAGA AGAAGCACAG ATGGCAGGCA CTCCTTCCTG TCACCTGCCT 
GGTCAGGACA CAGACCCTGA TGGGCCAAAG CTGCAAGTCC CCTGTCCTCC CCGCCCAGTG 
CCGCCCCTCT ACCCCCCGGG GCCCTTGGGG CATACAACAG AGACCCCTGC TGAGGTATTC 
CAGGCCCTGC AGCAGCTGGG GCACCAAGCG TTCCGCCCTG GACAAGAACA GGCAATCATG 
CGGATTCTCT CAGGCATCTC CACTCTGTTA GTGCTGCCCA CAGGCGCTGG CAAGTCTCTG 
TGCTACCAGC TGCCAGCACT GCTGTATGCC CAGCGAAGCC CCTGCCTCAC CCTGGTGGTC 
TCCCCTCTCC TGTCACTCAT GGATGACCAG GTGTCTGACC TGCCCGCATG TCTGAAGGCA 
GCCTGCCTCC ACTCGGGTAT GACCAAAAAA CAACGAGAAG CTGTCTTGAA AAAGGTAAAG 
GCGGCCCAGG TGCATGTGCT GCTTGTGTCC CCAGAGGCCT TGGTCGGGTG TGGGGCTAAG 
GGCCCTGCCA GCCTCCCCCA GGCCTCTGAA CTGCCTCCAA TCGCCTTCGC CTGCATCGAC 
GAGGTCCACT GCCTCTCCCA GTGGTCACAC AACTTCCGGC CCTGCTACCT GCGTGTTTGC 
AAAGTACTTC GGGAGCATCT GGGTGTGCAC TGCCTTTTGG GCCTCACAGC CACAGCCACA 
CGAAGCACTG CCCGTGACGT GGCCCAGCAC CTGGGCGTAG CCGAAGAGCT GGAGCTCAGT 
GGGTTAGCCA GCATCCCTGC TAATCTGCAC CTCTCCGTGT CCATGGACAG AGACTCAGAC 
CAGGCTCTGG TGACACTGCT GCAAGGTGAC CGCTTTTGTG CCCTGGACTC TGTCATCATT 
TACTGTACTC GACGAAAAGA TACAGAACGG GTGGCCGCCC TCCTCCGGAC CCGCCTGTCT 
ATGGCGACAG AGCCAAGGCC TAGAGGTTGT GGCCCTGAGG CTCTAGCTGA AGCATACCAT 
GCTGGCATGT GCAGCCAGGA ACGGCGACGC GTGCAACAGG CCTTCATGCA GGGCCACCTG 
CGGGTGGTGG TGGCCACGGT AGCCTTTGGG ATGGGGCTGG ACCGGCCAGA TGTCCGGGCC 
GTGCTGCACC TGGGACTGCC TCCAAACTTC GAGAGCTATG TGCAAGCTAT TGGCCGTGCA 
GGACGTGATG GGAAGCCTGC CCACTGTCAC CTGTTCCTGC AGCCCCAGGG TGAAGACCTT 
TGGGAGCTGC GCAGACATGC CCATGCCGAT GGCACTGACT TCTTGGCTGT GAAGAAGCTG 
GTGCAACGTG TATTTCCATC CTGCACATGC ACCCAGAGGC CTATGTCCAA ACCCCTGCTT 
GAGGAGACCA AAGAGCGCGA CAGCCAACAA ACACACCCTG CATTGCGACA GGCCTGCCCG 
GGCCACACGC GGGCATTCCC AGTGCAGTCT ACAGTACAGG CTCTGGACAT GACAGAGGAG 
GCTATTGAGA CTCTGCTGTG CTATCTGGAA CTCCACCCTC GGCGCTGGCT GAAGCTGCTG 
TCATGCACTT ATGCCCACTG TCACCTACGC TGCCCTGGGG GCAGTGCTCA GCTGCAAGCT 
CTGGCCCACA GGTGTCCCCC ATTGGCTGCA TGTTTGGCCA AGTACCCACC TAAAGACACG 
AGTCGGGGCC GCAGCTCCTT AGACTTTGAC ATGGTGGAGC TGGCAGACTC GATGGGCTGG 
GAGCTGGCTT CTGTACGGCG GGCTCTCCAC CAGCTGCGGT GGGACCAGGA GCCAAAGAAA 
GGTGCACCAC GGGGCACAGG GGTGCTGGTG GAGTTCAGCG AGTTGGCCTT TCATGTGCAC 
AGTCCTGGTG ACCTGACAGA TGAGGAGAAG GACCAGATCT GCGACTTCCT GTACAGCCGT 
GTACAGACTC GTGAACACAA GGCCCTGGCC CATCTACACC AAATATCTAA GGCCTTTCGC 
AGTGTGGCCT TTCCCAGCTG CGGACCCTGT CTAGAGCAGG CTGATGAGGA TCGCAGCAAC 
CAGTTAAAGA CCTTGCTCAG CCACTACTTT GAGGAAGAGG AAGAGGAAGC TGTGAAGGAT 
GAGCAGGGTC CAGAGCCTGG GCAGAGCGAG CTTCAGGACT GGGAGGACCA TATTCGCCGT 
GATATCCACC AGCTCCTGTC CCTGAGGCCA GAGGAGAGGT TTTCAGGACG GGCTGTGGCC 
CGCATCTTCC ATGGCATCGC AAGTCCCTGC TACCCAGCCC AGATATATGG GAAGGACCAG 
CGCTTCTGGA GAAAGTATCT GCATGTGAAC TTCCATGCCG TGATGCGCCT GGCCACAGAA 
GAACTGCTGA GAGGCCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005086063.1
CDS	44..3664
Translation	MERLASVRARLQEWERAFARLHGRRPAREDVEAAPEDTRALYREYRNLKHAARQAGDGHRSLEQSLAETAEEAQEPSCWGPHLNRAATQNTQPTPRQGPLGSVQDYGKRLKANLESNPKTAKLQTRPALSRKCQPRKRALSTVPTPRPPGPRTCPDETNDVLPRVAEPQPRLGHLQQLQASLSLRLSSLDPGWLERCQDRVSDLLEVPSACGLDLDGKESQPQISGKVNVLDPGTRSEGSSQSPGGLATQPQSPKPSNKTDRKWTRNESSEDRAQAQDSGQAGLLSEGARASAHEKDPPGEAASAPRPHNSSKRARTETAKDKAHASTRPAFLDRGNYIRLNMKHKCYVRVGASRGRLLRKQVWRQKWRKKAGQFGGGRAGAEAQDACFRCGQCGHWASQCPQAGSTRTFQEDSGRDDKRPVPTLEEEAQMAGTPSCHLPGQDTDPDGPKLQVPCPPRPVPPLYPPGPLGHTTETPAEVFQALQQLGHQAFRPGQEQAIMRILSGISTLLVLPTGAGKSLCYQLPALLYAQRSPCLTLVVSPLLSLMDDQVSDLPACLKAACLHSGMTKKQREAVLKKVKAAQVHVLLVSPEALVGCGAKGPASLPQASELPPIAFACIDEVHCLSQWSHNFRPCYLRVCKVLREHLGVHCLLGLTATATRSTARDVAQHLGVAEELELSGLASIPANLHLSVSMDRDSDQALVTLLQGDRFCALDSVIIYCTRRKDTERVAALLRTRLSMATEPRPRGCGPEALAEAYHAGMCSQERRRVQQAFMQGHLRVVVATVAFGMGLDRPDVRAVLHLGLPPNFESYVQAIGRAGRDGKPAHCHLFLQPQGEDLWELRRHAHADGTDFLAVKKLVQRVFPSCTCTQRPMSKPLLEETKERDSQQTHPALRQACPGHTRAFPVQSTVQALDMTEEAIETLLCYLELHPRRWLKLLSCTYAHCHLRCPGGSAQLQALAHRCPPLAACLAKYPPKDTSRGRSSLDFDMVELADSMGWELASVRRALHQLRWDQEPKKGAPRGTGVLVEFSELAFHVHSPGDLTDEEKDQICDFLYSRVQTREHKALAHLHQISKAFRSVAFPSCGPCLEQADEDRSNQLKTLLSHYFEEEEEEAVKDEQGPEPGQSELQDWEDHIRRDIHQLLSLRPEERFSGRAVARIFHGIASPCYPAQIYGKDQRFWRKYLHVNFHAVMRLATEELLRGR

Target ORF information:

RefSeq Version	XM_005086006.3
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus RecQ like helicase 4 (Recql4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005086006.3

ORF Insert Sequence:

ATGGAAAGAC TGGCGAGCGT TCGCGCGCGG CTGCAGGAGT GGGAACGCGC TTTCGCGCGG 
CTGCACGGGA GACGGCCAGC GCGGGAGGAC GTGGAGGCGG CCCCTGAGGA CACGCGCGCG 
CTCTACCGTG AGTACCGCAA CCTGAAGCAT GCAGCGCGCC AGGCCGGCGA CGGACACCGC 
AGCCTGGAGC AGTCTCTCGC CGAGACAGCT GAGGAGGCAC AGGAACCAAG CTGCTGGGGT 
CCCCACCTGA ATCGAGCCGC AACCCAGAAT ACACAGCCTA CGCCCAGGCA GGGCCCACTG 
GGTTCAGTAC AGGACTATGG GAAGAGGCTC AAAGCCAATC TGGAAAGCAA CCCGAAAACA 
GCAAAGCTGC AGACTAGGCC AGCCCTGAGC CGAAAATGCC AGCCTCGGAA GAGAGCCTTG 
TCCACCGTGC CCACCCCAAG GCCCCCAGGC CCAAGGACCT GTCCAGACGA AACTAATGAT 
GTACTTCCCA GGGTTGCTGA GCCCCAGCCA CGGCTAGGCC ATCTCCAGCA GCTTCAGGCA 
TCCCTGAGTC TGAGGCTGAG TTCCCTAGAT CCTGGCTGGT TAGAGCGATG TCAAGACAGA 
GTTTCAGACC TTCTAGAGGT CCCCAGTGCC TGTGGGCTTG ACCTGGATGG AAAGGAGTCA 
CAGCCCCAGA TATCAGGCAA GGTGAATGTC CTTGATCCTG GTACCCGTTC AGAAGGGTCT 
TCACAGAGCC CAGGGGGCCT AGCCACACAG CCACAGAGCC CCAAACCCAG CAACAAGACA 
GACAGGAAAT GGACGCGGAA TGAGAGTTCG GAGGACCGTG CACAAGCCCA GGACAGTGGC 
CAAGCAGGAC TGCTGTCTGA GGGAGCCCGG GCTAGCGCAC ATGAGAAAGA CCCTCCAGGA 
GAAGCTGCGA GCGCGCCCCG GCCCCACAAC TCCTCCAAGA GGGCCAGGAC AGAGACGGCC 
AAGGACAAAG CCCACGCCTC CACTAGACCC GCTTTCCTGG ACAGAGGGAA CTACATCCGA 
CTCAACATGA AACACAAGTG CTATGTACGA GTTGGCGCCA GTCGAGGCAG GCTTCTCCGG 
AAGCAGGTGT GGAGGCAGAA GTGGCGGAAG AAAGCGGGGC AGTTTGGGGG CGGCAGAGCC 
GGAGCCGAAG CCCAGGACGC CTGTTTCCGG TGTGGGCAGT GTGGTCACTG GGCTTCCCAG 
TGCCCCCAGG CAGGCTCCAC CCGGACCTTC CAGGAAGACA GTGGCAGGGA TGACAAACGG 
CCCGTTCCCA CTTTGGAAGA AGAAGCACAG ATGGCAGGCA CTCCTTCCTG TCACCTGCCT 
GGTCAGGACA CAGACCCTGA TGGGCCAAAG CTGCAAGTCC CCTGTCCTCC CCGCCCAGTG 
CCGCCCCTCT ACCCCCCGGG GCCCTTGGGG CATACAACAG AGACCCCTGC TGAGGTATTC 
CAGGCCCTGC AGCAGCTGGG GCACCAAGCG TTCCGCCCTG GACAAGAACA GGCAATCATG 
CGGATTCTCT CAGGCATCTC CACTCTGTTA GTGCTGCCCA CAGGCGCTGG CAAGTCTCTG 
TGCTACCAGC TGCCAGCACT GCTGTATGCC CAGCGAAGCC CCTGCCTCAC CCTGGTGGTC 
TCCCCTCTCC TGTCACTCAT GGATGACCAG GTGTCTGACC TGCCCGCATG TCTGAAGGCA 
GCCTGCCTCC ACTCGGGTAT GACCAAAAAA CAACGAGAAG CTGTCTTGAA AAAGGTAAAG 
GCGGCCCAGG TGCATGTGCT GCTTGTGTCC CCAGAGGCCT TGGTCGGGTG TGGGGCTAAG 
GGCCCTGCCA GCCTCCCCCA GGCCTCTGAA CTGCCTCCAA TCGCCTTCGC CTGCATCGAC 
GAGGTCCACT GCCTCTCCCA GTGGTCACAC AACTTCCGGC CCTGCTACCT GCGTGTTTGC 
AAAGTACTTC GGGAGCATCT GGGTGTGCAC TGCCTTTTGG GCCTCACAGC CACAGCCACA 
CGAAGCACTG CCCGTGACGT GGCCCAGCAC CTGGGCGTAG CCGAAGAGCT GGAGCTCAGT 
GGGTTAGCCA GCATCCCTGC TAATCTGCAC CTCTCCGTGT CCATGGACAG AGACTCAGAC 
CAGGCTCTGG TGACACTGCT GCAAGGTGAC CGCTTTTGTG CCCTGGACTC TGTCATCATT 
TACTGTACTC GACGAAAAGA TACAGAACGG GTGGCCGCCC TCCTCCGGAC CCGCCTGTCT 
ATGGCGACAG AGCCAAGGCC TAGAGGTTGT GGCCCTGAGG CTCTAGCTGA AGCATACCAT 
GCTGGCATGT GCAGCCAGGA ACGGCGACGC GTGCAACAGG CCTTCATGCA GGGCCACCTG 
CGGGTGGTGG TGGCCACGGT AGCCTTTGGG ATGGGGCTGG ACCGGCCAGA TGTCCGGGCC 
GTGCTGCACC TGGGACTGCC TCCAAACTTC GAGAGCTATG TGCAAGCTAT TGGCCGTGCA 
GGACGTGATG GGAAGCCTGC CCACTGTCAC CTGTTCCTGC AGCCCCAGGG TGAAGACCTT 
TGGGAGCTGC GCAGACATGC CCATGCCGAT GGCACTGACT TCTTGGCTGT GAAGAAGCTG 
GTGCAACGTG TATTTCCATC CTGCACATGC ACCCAGAGGC CTATGTCCAA ACCCCTGCTT 
GAGGAGACCA AAGAGCGCGA CAGCCAACAA ACACACCCTG CATTGCGACA GGCCTGCCCG 
GGCCACACGC GGGCATTCCC AGTGCAGTCT ACAGTACAGG CTCTGGACAT GACAGAGGAG 
GCTATTGAGA CTCTGCTGTG CTATCTGGAA CTCCACCCTC GGCGCTGGCT GAAGCTGCTG 
TCATGCACTT ATGCCCACTG TCACCTACGC TGCCCTGGGG GCAGTGCTCA GCTGCAAGCT 
CTGGCCCACA GGTGTCCCCC ATTGGCTGCA TGTTTGGCCA AGTACCCACC TAAAGACACG 
AGTCGGGGCC GCAGCTCCTT AGACTTTGAC ATGGTGGAGC TGGCAGACTC GATGGGCTGG 
GAGCTGGCTT CTGTACGGCG GGCTCTCCAC CAGCTGCGGT GGGACCAGGA GCCAAAGAAA 
GGTGCACCAC GGGGCACAGG GGTGCTGGTG GAGTTCAGCG AGTTGGCCTT TCATGTGCAC 
AGTCCTGGTG ACCTGACAGA TGAGGAGAAG GACCAGATCT GCGACTTCCT GTACAGCCGT 
GTACAGACTC GTGAACACAA GGCCCTGGCC CATCTACACC AAATATCTAA GGCCTTTCGC 
AGTGTGGCCT TTCCCAGCTG CGGACCCTGT CTAGAGCAGG CTGATGAGGA TCGCAGCAAC 
CAGTTAAAGA CCTTGCTCAG CCACTACTTT GAGGAAGAGG AAGAGGAAGC TGTGAAGGAT 
GAGCAGGGTC CAGAGCCTGG GCAGAGCGAG CTTCAGGACT GGGAGGACCA TATTCGCCGT 
GATATCCACC AGCTCCTGTC CCTGAGGCCA GAGGAGAGGT TTTCAGGACG GGCTGTGGCC 
CGCATCTTCC ATGGCATCGC AAGTCCCTGC TACCCAGCCC AGATATATGG GAAGGACCAG 
CGCTTCTGGA GAAAGTATCT GCATGTGAAC TTCCATGCCG TGATGCGCCT GGCCACAGAA 
GAACTGCTGA GAGGCCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Recql4 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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