Nup214 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nup214
Entrez Gene ID	101827647
Full Name	nucleoporin 214
Gene Type	protein-coding
Organism	Mesocricetus auratus(golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013126431.1	XP_012981885.1	nuclear pore complex protein Nup214
XM_021223885.1	XP_021079544.1	nuclear pore complex protein Nup214 isoform X2
XM_013126431.2	XP_012981885.1	nuclear pore complex protein Nup214 isoform X1

Pathways from BioSystems
Gene Ontology

The following Nup214 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup214 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe142310	XM_013126431.2 Latest version!	Mesocricetus auratus nucleoporin 214 (Nup214), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMe255878	XM_021223885.1 Latest version!	Mesocricetus auratus nucleoporin 214 (Nup214), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OMe142310	XM_013126431.1	Mesocricetus auratus nucleoporin 214kDa (Nup214), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nup214 ( XM_013126431.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012981885.1 Mesocricetus auratus nucleoporin 214kDa (Nup214), mRNA.

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CloneID	OMe142310
Clone ID Related Accession (Same CDS sequence)	XM_013126431.1 , XM_013126431.2
Accession Version	XM_013126431.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	nuclear pore complex protein Nup214
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801898.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACG ATATGGATGC CATAGTCCCT GAGCGGGAGA TGAAGGACTT TCAGTTTAGA 
GCACTGAAGA AAGTAAGGAT CTTTGACTCT CCTGAAGAGT TGCCCAAGGC GCGCTCGAGT 
ATGCTTGCCA TATCCAATAA GTATGGTATG CTCTTTGCTG GTGGCGCCGG TGGCTTGCAT 
GTTTTTCCTA CTAAAAGTCT TCTTATCCAA AACAGACCTG GAGATGATCC CAATAAAATT 
GTTGAGCCAG TCGACAGCTT GAATGTTGCT CTGAAATTCC CAGTACATCA CCTGGCACTG 
AGCTGTGACA ACCTCACACT CTCTGCATGC ATGGTGTCCA GTGAATACGG CTCCGTTATA 
GCTTTTTTTG ATGTTCGCAC ATTTTCGAAT CAGGCTAAAC CAGTGAAACT CCCATTTACC 
TATCACAAGC TTTCAAAGGA TGTAGGTGGT ATGGTGACTG ATATGAAGTG GAACCCCACT 
GTCCCGTCCA TGGTGGCAGT TTGTCTGGCT GATGGCAGTA TTGCTGTCCT GCAAGTTACA 
GAAGTGGTGA AAGTGTGTGC CACTCTTCCT CCCTCGGCAG CAGTTACATG TGTGTGCTGG 
AGTCCCAAAG GAAAGCAGCT GGCAGTAGGG AAGCAGAATG GAACCGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCA TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTAAGAGTCC TGGATGTGCT GTGGATTGGC ACCTATGTCT TCATGATAGT TTATGCTGGT 
GCAGATGTGA CCATGGAAAC CTGTCCAGAT GTGGTGATGG CTTTACTCCC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GCTACAGCAG CTGCACAGAG 
CGACAGCATC ATTACTACCT CAATTACATC GAGGAGTGGG ATTTGGTGCT GGCAGCATCT 
GCAGCGTCAA CAGAAGTTAG CATCCTTGCT CGACAAAGTG ATCAGACTAA TTGGGAATCT 
TGGCTGCTGG AGGACTCTAG TCGAGCTGAA CTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GCGTTGCCAT AGATTATACT AATGAAGTAG AAGTCGTCAT CAACGATGAA 
AAGACCCTTC CTGCTGCTCC GGTTCTCCTG TTACTTTCAA CAGATGGTGT GCTTTGTCCG 
TTTTATATGA TCAATCAAAA TCCTGGGGTT AGGTCCCTAA TCAAGACCAT GGAGCTGGCC 
ACAATAGAAG GAGAGCGACA GCCCAAGTCC TCAGAAAGTT TTCCTACCAC TCCAACCTCC 
CCTCAAGCCC CGGAGAACCT TGATGTCCCA GAAGAAGCCT CTTTGTCCCT TGCACCGGTG 
TCCTCTGCTG TTCCTACCAC CACCTTCCCT TTGCCCTCTT CTGGGGGGGC CCCTTGTGTG 
TTCTCCTTTG GCCCCTCATC CTTAAAGCCA TCTGTGTCTG TGGCTGGGGA GCCTCCTCTG 
GTTCCCTCTG GCTCCGACAA TCCCAGAGCA GCTCTGGGCT CTGGCACCTC CACCTTCCCC 
TTTGCCCCTC CTTCCAAAGG CCCCCAGGCC CTTAACCCTG CAGCACCCTC CATGGCCACA 
CCTGCTGCTC CCTTCACTTT TGGATCATCA GGTTTTAAGC CCACCTTGGA AAGCACACCA 
ATGTCCAGTG CTCCAAACAC AGGGATGAAG CCTTCGTTCC CACCCTCAGC CTCTTCTGTG 
AAAGTCAACC TGAATGAAAA GTTCACCGCT GTGGCCTCTG CGCCTGTCCA TAGCTCCACG 
AGCACACCCT CCCTGTTGCC ATTCTCTTCC TCCCCCAAGC CAGCTGCTTC TGGACAGCTC 
AGCCATCCCA CGCCTCTGCC GGCATCGTCC AGCTCCATTC CTTTGAAGTC TTCAGTCTCT 
CCCTCGCCAT CAGGCCGATC TACTCCGAGT GTTCCAAGCT CAGTTCCCTC CATGGTACAG 
AAATCACCCA GGATAACCCC TCCAGTGGTA AAGTCAGGCT CTCCTCAGGC TAAGTCCCTT 
CAGCCTCCTG TCACAGAGAA ACAGGGACAT CAGTGGAAGG ATTCAGACCC TGTGATGGCT 
GGCATTGGAG AAGAGATTGC ACATTTCCAG AAAGAGCTAG AGGAGTTAAA GACCCGGACC 
TCCAAAGCCT GTTTCCAGGT TGGCACCTTG GAGGAGATGA AGACCCTGCG GACAGAATCT 
GATGACTTGC ACACCTTCCT CTTCGAGATT AGAGAGGCCA CAGAGTCTCT TCATGGAGAC 
ATAAGTACCC TGAAAACAAC TCTGCTGGAG GGCTTTGCTG GTGTTGAAGA AGCCAGAGAA 
CAAAACGGAA GAAACCATGA CTCTGGGTAT CTGCACCTGC TTTATAAAAG ACCATTGGAT 
CCCAAGAGTG AAGCTCAGCT CCAGGAAATC CGTCGTCTTC ATCAGTATGT GAAGTTTGCT 
GTCCAGGATG TAAATGATGT TTTAGACCTG GAGTGGGATC GGCATCTGGA ACAGAAGAAA 
AGACAGAGGC GTTTGCTTGT GCCAGAACGA GAGACACTTT TCAACACACT AGCCAACAAC 
CGGGAGATCA TCAACCAACA GAGGAAGAGG TTGAATCACC TGGTGGACAG TCTGCAGCAG 
CTCCGTCTCT ATAACCAGAC CCCCCCATGG AATCTGCCCT CAACACTCTC AACTCAGAGC 
AGCACTCACA GTTTTGACAG TGACCTTGAA AGCCTGCTAA AAACAACCGT TGAATCTCAC 
ACCAAGCCCT CTTCCAGAGT GCAAGCTAAA CTGTCCCCCG CTAAACAGGC CCAACTGAGG 
AACTTCTTAG CCAAAAGGAA GACCCCACCA GTGAGGTCCA CTGCTCCAGC CAGCCTGTCT 
CGGTCAGCCT TCCTGTCTCA GAGATATTAT GAAGACTTAG ATGAAGCCAT CTCCACATCC 
TCCATCTGTC AGCCCCTGGA GGGAGAAGAT GCCCGGTCTC CCTGCAAAGA GGAGGAAGCT 
GTGGTCCAGG TCCATAGGCA TGCTCCTGTG GTCCGCACGC CTTCTATCCA ACCCAGTCTC 
CTGCCCCAGG CAATGCCTTT TGCTAAGCCT CATCTGTTTC ATAGTTCTTC ACCTGCTGTG 
ATGAGCTCCT CAGTCTCTAC ATCAGCCAGC AAAGTTATTC CTCCAGGAGC CGACAGCACG 
ATGCTGGCAA CCAAAACTGT TAAGCATGGT GCACCAGGTC CTTCCCACAC TGTCGCAGCT 
TCCCAGGCGG CAGCAGCTGC AGCCATGAGA CGGCAGATGG CCAATCAGGT GCCAGCTGTG 
AGCACTCTCA CCGAATCAAC TTTGAAGAAT GTTCCTCAAG TGGTAAACGT ACAGGAACTG 
AAGAGTAGCC CTGCAGCCCC GTCTGTGGCC CTGGGTTCTT CAGTGCCGCA CTCAACAGCC 
AAAGCACCCC ATGCAGTGTT GGCCCCGGTG GCTGCCAGCC AAGCTAAGCA GGGCTCTCTG 
ATAAATTCCT TCAAGCCGTC AGGGACCACA CCAGCATCCT GTCAATTGTC CTCTGGAGAT 
AAAGCTGCAG GTTCAGGCAC AGCCAAGACG GAATCAACCG TGACCTCGAC TCCATCTGCT 
GTGGGGCAGC CCAACAAACC TTTCTCATTT GCTCCCTCAG GGACTGCCTT TACTTTTGGG 
ACGATCGCAT CGGCGCCCTC TTCTAGTCTC GCTGCCACAC CAGGATCAGT ACCCCCCACG 
AAAGAGTCAA ACCAGCTGGA GGCATTTTCA TTTGGCGGGG GAGGCAAACC TTTCTGTGAG 
GCCATTCCTG GAAGCTCTCC TCCCACAGGA GCCTTGTCAG CAGCACCCAG CACTTCTGTA 
ACGACTGCCT CTCCAGGAGA CTCTGTCCCA CCCGGCAGCA GACCTGCAGC TTCCTCTGGA 
ATTACTGTGT CCACTGCCTC TAGCAAATTG GAAACTCCAA CGTCTAAGCT GGGAGAGTTC 
CTGTTCCCAG GCTCCCTGAC TGGAGAAACG CTAGGGAGCT TTTCAGGACT GCGTGTTGGC 
CAAGCTGAGG ACTCTGCAAA GCCAGTCAGT AAGGCCTCAT CCATGAGCCT AGCTGGATCC 
CCGACACCAA AGCCCCCAGG CTTTAGCTTT CCCGGCACTC CAGTGTTAGG GAAGCCTGTG 
GAGCCCACTG TGACATCCTC TGTAACCCCT GCCCCGGCAG CGCCAGTGTC TTCCCTGGGT 
GTGAGCACTA ACAGTTCGGC TGCGCCAGTC TTTGCCAGTC TGCCCCTTAG CAGTGCCGGA 
CCTTCTGGGC TCATCAGTTT TGGTGGGGCA TCTCTGGCCG GTAGCAAAGC TGGTTTCGCA 
TTTGGAAACC AACAGACTAG TAGTACTGTG TCATCAGCTG CCCCAACATC CACCTCAGTT 
GCCCCTTCCC TTCCAGCGTC TTTCCCCACC TTATCATTTG GTGGCCTCCT AAGTTCAACG 
TCTACTTCTT CCCTTCCTGT GTCCTCTGGT AAGAGCCCAG AGGAGGCTAC ACCATCAGCT 
GTGCCTGATA AGTCAGGCCA CAGTGAAGTC TCAGCATCAG CACCCTCCCT TCCAGTACAG 
CCACAATCCG CCCAGCCTCC CCAGGCTTCT CTGCAAACTT CTGACCCTGT CAAAACAGAG 
CCTGTTCTTG TGCAGGCCAC AGACAGCAGC CCCAACCCAC CATCATCTGC AGCCAGTGTT 
GTGGCATCTA CAGAGGCCAC TCCAGTAGCC CCAGGGGGCC CTGACAGCAA GACAGAGGCT 
ACTGCTCCTG CGTCTACCTT TGCGGGGCCT GGGCAGGCTG CTGTCGCAGC GGCTGTTCTC 
CCAGGGACAG GCCCAGCAGC CAGTGAAGCA TCGGGTACTC CTAGCACCTC CAGCACAGCT 
TCCAGCAGCG TCCCAGCTTC TGCCACAGAG ACAGCCGCGT TTGGGACTGC TACTTCTGGC 
TCCTCGGTCT GCACACAGCC ACCTGCTGCC AGTTCTAGCT CAGCGTTCAG CCAACTCTCC 
AGTAACACAG CCACTGCATC CTCTGCCACC CCCATGTTTG GGCAGGTGGC AGCTAGCAGC 
ACAGCCACTG CCACACCACA GGCCAGCAGC TCAGGGTTTG GCAGCCCTGC GTTTGGTGCT 
TCAGCTCCAG CAGTCTTTGG ACAGACAGCC TTTGGGCAGG CCCCGGCCTT TGGGCAAGCA 
GCAAGCAACC CAGCAAGTGG CTTCTCTTTC AGCCAGCCTG GGTTCAGCTC TGGGCCTGTT 
TTTGGCCAGT CTGTTTCCTC TACCCCCACA TCCACCAGTG CGAATGTCTT CGGAGCCGCC 
TCGAGTGCCA GCAGCCCTGG TTCCTTCTCA TTTGGACAGG CTTCTACCAA CACAGGAGGG 
ACACTGTTTG GCCAGAACAA CCCCCCTGCT TTTGGTCAAA GCCCTGGCTT TGGACAGGGA 
GGCTCTGTGT TTGGTAGCAC CTCGGCCACT ACCTCTACAG CAGCACCTTC AGGGTTTAGC 
TTTTGTCAAG CCTCTGGTTT TGGGTCTAGT AATACTGGTT CTGTGTTTGG TCAGGCATCC 
AGTACTGGCG GATCAGTCTT TGGCCAGCAG TCATCTACTT CCAGTGGTAG TGTGTTTGGG 
TCTGGAAACT CGACAAGAGG GAGCGGTTTC TTCAGTGGCC TTGGAGGAAA ACCCAGCCAG 
GATGCTGCCA ACAAAAACCC ATTCAGCTCA GCCAGTGGGG GCTTTGGATC TACAACCACC 
CCAAATACCG CTAATCTATT TGGAAACAGT GGCGCTAAGA CATTTGGAGG GTTTGGCAGC 
TCATCCTTTG GGGAACAGAA GCCTGCTGGC ACTTTCAGCT CTGGTGGTGG AAGCGTGGCA 
TCCCAAGGCT TTGGATTTTC TACTCCAAAT AAAACAGGTG GCTTTGGTGC TGCTCCAGTG 
TTTGGCAGCC CTCCTACTTT TGGGGGATCC CCTGGGTTTG GAGGGGTGCC AGCATTCGGT 
TCAGCCCCGG CCTTTACAAG CCCTCTGGGC TCGACGGGAG GCAAAGTGTT CGGAGAGGGT 
ACTGCGGCTG CCAGCGCAGG AGGATTCGGG TTTGGGAGCA GCAGCAGCAC TGCATCCTTT 
GGTACCCTCG CCAGTCAGAA CGCGCCAACC TTCGGGTCCT TGTCCCAGCA GACCTCAGGC 
TTTGGGACCC AGAACAGTGG CTTCTCTGGG TTTGGATCGG GCACAGGAGC GTTCGCCTTT 
GGATCATCGA ACTCGTCTGT CCAAGGCTTT GGTGGCTGGA GAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012981885.1
CDS	99..6326
Translation	MGDDMDAIVPEREMKDFQFRALKKVRIFDSPEELPKARSSMLAISNKYGMLFAGGAGGLHVFPTKSLLIQNRPGDDPNKIVEPVDSLNVALKFPVHHLALSCDNLTLSACMVSSEYGSVIAFFDVRTFSNQAKPVKLPFTYHKLSKDVGGMVTDMKWNPTVPSMVAVCLADGSIAVLQVTEVVKVCATLPPSAAVTCVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFMIVYAGADVTMETCPDVVMALLPKKEEKHPEIFVNFMEPCYSSCTERQHHYYLNYIEEWDLVLAASAASTEVSILARQSDQTNWESWLLEDSSRAELPVTDKSDDSLPMGVAIDYTNEVEVVINDEKTLPAAPVLLLLSTDGVLCPFYMINQNPGVRSLIKTMELATIEGERQPKSSESFPTTPTSPQAPENLDVPEEASLSLAPVSSAVPTTTFPLPSSGGAPCVFSFGPSSLKPSVSVAGEPPLVPSGSDNPRAALGSGTSTFPFAPPSKGPQALNPAAPSMATPAAPFTFGSSGFKPTLESTPMSSAPNTGMKPSFPPSASSVKVNLNEKFTAVASAPVHSSTSTPSLLPFSSSPKPAASGQLSHPTPLPASSSSIPLKSSVSPSPSGRSTPSVPSSVPSMVQKSPRITPPVVKSGSPQAKSLQPPVTEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKTRTSKACFQVGTLEEMKTLRTESDDLHTFLFEIREATESLHGDISTLKTTLLEGFAGVEEAREQNGRNHDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKKRQRRLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYNQTPPWNLPSTLSTQSSTHSFDSDLESLLKTTVESHTKPSSRVQAKLSPAKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEAISTSSICQPLEGEDARSPCKEEEAVVQVHRHAPVVRTPSIQPSLLPQAMPFAKPHLFHSSSPAVMSSSVSTSASKVIPPGADSTMLATKTVKHGAPGPSHTVAASQAAAAAAMRRQMANQVPAVSTLTESTLKNVPQVVNVQELKSSPAAPSVALGSSVPHSTAKAPHAVLAPVAASQAKQGSLINSFKPSGTTPASCQLSSGDKAAGSGTAKTESTVTSTPSAVGQPNKPFSFAPSGTAFTFGTIASAPSSSLAATPGSVPPTKESNQLEAFSFGGGGKPFCEAIPGSSPPTGALSAAPSTSVTTASPGDSVPPGSRPAASSGITVSTASSKLETPTSKLGEFLFPGSLTGETLGSFSGLRVGQAEDSAKPVSKASSMSLAGSPTPKPPGFSFPGTPVLGKPVEPTVTSSVTPAPAAPVSSLGVSTNSSAAPVFASLPLSSAGPSGLISFGGASLAGSKAGFAFGNQQTSSTVSSAAPTSTSVAPSLPASFPTLSFGGLLSSTSTSSLPVSSGKSPEEATPSAVPDKSGHSEVSASAPSLPVQPQSAQPPQASLQTSDPVKTEPVLVQATDSSPNPPSSAASVVASTEATPVAPGGPDSKTEATAPASTFAGPGQAAVAAAVLPGTGPAASEASGTPSTSSTASSSVPASATETAAFGTATSGSSVCTQPPAASSSSAFSQLSSNTATASSATPMFGQVAASSTATATPQASSSGFGSPAFGASAPAVFGQTAFGQAPAFGQAASNPASGFSFSQPGFSSGPVFGQSVSSTPTSTSANVFGAASSASSPGSFSFGQASTNTGGTLFGQNNPPAFGQSPGFGQGGSVFGSTSATTSTAAPSGFSFCQASGFGSSNTGSVFGQASSTGGSVFGQQSSTSSGSVFGSGNSTRGSGFFSGLGGKPSQDAANKNPFSSASGGFGSTTTPNTANLFGNSGAKTFGGFGSSSFGEQKPAGTFSSGGGSVASQGFGFSTPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSSTASFGTLASQNAPTFGSLSQQTSGFGTQNSGFSGFGSGTGAFAFGSSNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_013126431.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus nucleoporin 214kDa (Nup214), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013126431.1

ORF Insert Sequence:

ATGGGAGACG ATATGGATGC CATAGTCCCT GAGCGGGAGA TGAAGGACTT TCAGTTTAGA 
GCACTGAAGA AAGTAAGGAT CTTTGACTCT CCTGAAGAGT TGCCCAAGGC GCGCTCGAGT 
ATGCTTGCCA TATCCAATAA GTATGGTATG CTCTTTGCTG GTGGCGCCGG TGGCTTGCAT 
GTTTTTCCTA CTAAAAGTCT TCTTATCCAA AACAGACCTG GAGATGATCC CAATAAAATT 
GTTGAGCCAG TCGACAGCTT GAATGTTGCT CTGAAATTCC CAGTACATCA CCTGGCACTG 
AGCTGTGACA ACCTCACACT CTCTGCATGC ATGGTGTCCA GTGAATACGG CTCCGTTATA 
GCTTTTTTTG ATGTTCGCAC ATTTTCGAAT CAGGCTAAAC CAGTGAAACT CCCATTTACC 
TATCACAAGC TTTCAAAGGA TGTAGGTGGT ATGGTGACTG ATATGAAGTG GAACCCCACT 
GTCCCGTCCA TGGTGGCAGT TTGTCTGGCT GATGGCAGTA TTGCTGTCCT GCAAGTTACA 
GAAGTGGTGA AAGTGTGTGC CACTCTTCCT CCCTCGGCAG CAGTTACATG TGTGTGCTGG 
AGTCCCAAAG GAAAGCAGCT GGCAGTAGGG AAGCAGAATG GAACCGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCA TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTAAGAGTCC TGGATGTGCT GTGGATTGGC ACCTATGTCT TCATGATAGT TTATGCTGGT 
GCAGATGTGA CCATGGAAAC CTGTCCAGAT GTGGTGATGG CTTTACTCCC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GCTACAGCAG CTGCACAGAG 
CGACAGCATC ATTACTACCT CAATTACATC GAGGAGTGGG ATTTGGTGCT GGCAGCATCT 
GCAGCGTCAA CAGAAGTTAG CATCCTTGCT CGACAAAGTG ATCAGACTAA TTGGGAATCT 
TGGCTGCTGG AGGACTCTAG TCGAGCTGAA CTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GCGTTGCCAT AGATTATACT AATGAAGTAG AAGTCGTCAT CAACGATGAA 
AAGACCCTTC CTGCTGCTCC GGTTCTCCTG TTACTTTCAA CAGATGGTGT GCTTTGTCCG 
TTTTATATGA TCAATCAAAA TCCTGGGGTT AGGTCCCTAA TCAAGACCAT GGAGCTGGCC 
ACAATAGAAG GAGAGCGACA GCCCAAGTCC TCAGAAAGTT TTCCTACCAC TCCAACCTCC 
CCTCAAGCCC CGGAGAACCT TGATGTCCCA GAAGAAGCCT CTTTGTCCCT TGCACCGGTG 
TCCTCTGCTG TTCCTACCAC CACCTTCCCT TTGCCCTCTT CTGGGGGGGC CCCTTGTGTG 
TTCTCCTTTG GCCCCTCATC CTTAAAGCCA TCTGTGTCTG TGGCTGGGGA GCCTCCTCTG 
GTTCCCTCTG GCTCCGACAA TCCCAGAGCA GCTCTGGGCT CTGGCACCTC CACCTTCCCC 
TTTGCCCCTC CTTCCAAAGG CCCCCAGGCC CTTAACCCTG CAGCACCCTC CATGGCCACA 
CCTGCTGCTC CCTTCACTTT TGGATCATCA GGTTTTAAGC CCACCTTGGA AAGCACACCA 
ATGTCCAGTG CTCCAAACAC AGGGATGAAG CCTTCGTTCC CACCCTCAGC CTCTTCTGTG 
AAAGTCAACC TGAATGAAAA GTTCACCGCT GTGGCCTCTG CGCCTGTCCA TAGCTCCACG 
AGCACACCCT CCCTGTTGCC ATTCTCTTCC TCCCCCAAGC CAGCTGCTTC TGGACAGCTC 
AGCCATCCCA CGCCTCTGCC GGCATCGTCC AGCTCCATTC CTTTGAAGTC TTCAGTCTCT 
CCCTCGCCAT CAGGCCGATC TACTCCGAGT GTTCCAAGCT CAGTTCCCTC CATGGTACAG 
AAATCACCCA GGATAACCCC TCCAGTGGTA AAGTCAGGCT CTCCTCAGGC TAAGTCCCTT 
CAGCCTCCTG TCACAGAGAA ACAGGGACAT CAGTGGAAGG ATTCAGACCC TGTGATGGCT 
GGCATTGGAG AAGAGATTGC ACATTTCCAG AAAGAGCTAG AGGAGTTAAA GACCCGGACC 
TCCAAAGCCT GTTTCCAGGT TGGCACCTTG GAGGAGATGA AGACCCTGCG GACAGAATCT 
GATGACTTGC ACACCTTCCT CTTCGAGATT AGAGAGGCCA CAGAGTCTCT TCATGGAGAC 
ATAAGTACCC TGAAAACAAC TCTGCTGGAG GGCTTTGCTG GTGTTGAAGA AGCCAGAGAA 
CAAAACGGAA GAAACCATGA CTCTGGGTAT CTGCACCTGC TTTATAAAAG ACCATTGGAT 
CCCAAGAGTG AAGCTCAGCT CCAGGAAATC CGTCGTCTTC ATCAGTATGT GAAGTTTGCT 
GTCCAGGATG TAAATGATGT TTTAGACCTG GAGTGGGATC GGCATCTGGA ACAGAAGAAA 
AGACAGAGGC GTTTGCTTGT GCCAGAACGA GAGACACTTT TCAACACACT AGCCAACAAC 
CGGGAGATCA TCAACCAACA GAGGAAGAGG TTGAATCACC TGGTGGACAG TCTGCAGCAG 
CTCCGTCTCT ATAACCAGAC CCCCCCATGG AATCTGCCCT CAACACTCTC AACTCAGAGC 
AGCACTCACA GTTTTGACAG TGACCTTGAA AGCCTGCTAA AAACAACCGT TGAATCTCAC 
ACCAAGCCCT CTTCCAGAGT GCAAGCTAAA CTGTCCCCCG CTAAACAGGC CCAACTGAGG 
AACTTCTTAG CCAAAAGGAA GACCCCACCA GTGAGGTCCA CTGCTCCAGC CAGCCTGTCT 
CGGTCAGCCT TCCTGTCTCA GAGATATTAT GAAGACTTAG ATGAAGCCAT CTCCACATCC 
TCCATCTGTC AGCCCCTGGA GGGAGAAGAT GCCCGGTCTC CCTGCAAAGA GGAGGAAGCT 
GTGGTCCAGG TCCATAGGCA TGCTCCTGTG GTCCGCACGC CTTCTATCCA ACCCAGTCTC 
CTGCCCCAGG CAATGCCTTT TGCTAAGCCT CATCTGTTTC ATAGTTCTTC ACCTGCTGTG 
ATGAGCTCCT CAGTCTCTAC ATCAGCCAGC AAAGTTATTC CTCCAGGAGC CGACAGCACG 
ATGCTGGCAA CCAAAACTGT TAAGCATGGT GCACCAGGTC CTTCCCACAC TGTCGCAGCT 
TCCCAGGCGG CAGCAGCTGC AGCCATGAGA CGGCAGATGG CCAATCAGGT GCCAGCTGTG 
AGCACTCTCA CCGAATCAAC TTTGAAGAAT GTTCCTCAAG TGGTAAACGT ACAGGAACTG 
AAGAGTAGCC CTGCAGCCCC GTCTGTGGCC CTGGGTTCTT CAGTGCCGCA CTCAACAGCC 
AAAGCACCCC ATGCAGTGTT GGCCCCGGTG GCTGCCAGCC AAGCTAAGCA GGGCTCTCTG 
ATAAATTCCT TCAAGCCGTC AGGGACCACA CCAGCATCCT GTCAATTGTC CTCTGGAGAT 
AAAGCTGCAG GTTCAGGCAC AGCCAAGACG GAATCAACCG TGACCTCGAC TCCATCTGCT 
GTGGGGCAGC CCAACAAACC TTTCTCATTT GCTCCCTCAG GGACTGCCTT TACTTTTGGG 
ACGATCGCAT CGGCGCCCTC TTCTAGTCTC GCTGCCACAC CAGGATCAGT ACCCCCCACG 
AAAGAGTCAA ACCAGCTGGA GGCATTTTCA TTTGGCGGGG GAGGCAAACC TTTCTGTGAG 
GCCATTCCTG GAAGCTCTCC TCCCACAGGA GCCTTGTCAG CAGCACCCAG CACTTCTGTA 
ACGACTGCCT CTCCAGGAGA CTCTGTCCCA CCCGGCAGCA GACCTGCAGC TTCCTCTGGA 
ATTACTGTGT CCACTGCCTC TAGCAAATTG GAAACTCCAA CGTCTAAGCT GGGAGAGTTC 
CTGTTCCCAG GCTCCCTGAC TGGAGAAACG CTAGGGAGCT TTTCAGGACT GCGTGTTGGC 
CAAGCTGAGG ACTCTGCAAA GCCAGTCAGT AAGGCCTCAT CCATGAGCCT AGCTGGATCC 
CCGACACCAA AGCCCCCAGG CTTTAGCTTT CCCGGCACTC CAGTGTTAGG GAAGCCTGTG 
GAGCCCACTG TGACATCCTC TGTAACCCCT GCCCCGGCAG CGCCAGTGTC TTCCCTGGGT 
GTGAGCACTA ACAGTTCGGC TGCGCCAGTC TTTGCCAGTC TGCCCCTTAG CAGTGCCGGA 
CCTTCTGGGC TCATCAGTTT TGGTGGGGCA TCTCTGGCCG GTAGCAAAGC TGGTTTCGCA 
TTTGGAAACC AACAGACTAG TAGTACTGTG TCATCAGCTG CCCCAACATC CACCTCAGTT 
GCCCCTTCCC TTCCAGCGTC TTTCCCCACC TTATCATTTG GTGGCCTCCT AAGTTCAACG 
TCTACTTCTT CCCTTCCTGT GTCCTCTGGT AAGAGCCCAG AGGAGGCTAC ACCATCAGCT 
GTGCCTGATA AGTCAGGCCA CAGTGAAGTC TCAGCATCAG CACCCTCCCT TCCAGTACAG 
CCACAATCCG CCCAGCCTCC CCAGGCTTCT CTGCAAACTT CTGACCCTGT CAAAACAGAG 
CCTGTTCTTG TGCAGGCCAC AGACAGCAGC CCCAACCCAC CATCATCTGC AGCCAGTGTT 
GTGGCATCTA CAGAGGCCAC TCCAGTAGCC CCAGGGGGCC CTGACAGCAA GACAGAGGCT 
ACTGCTCCTG CGTCTACCTT TGCGGGGCCT GGGCAGGCTG CTGTCGCAGC GGCTGTTCTC 
CCAGGGACAG GCCCAGCAGC CAGTGAAGCA TCGGGTACTC CTAGCACCTC CAGCACAGCT 
TCCAGCAGCG TCCCAGCTTC TGCCACAGAG ACAGCCGCGT TTGGGACTGC TACTTCTGGC 
TCCTCGGTCT GCACACAGCC ACCTGCTGCC AGTTCTAGCT CAGCGTTCAG CCAACTCTCC 
AGTAACACAG CCACTGCATC CTCTGCCACC CCCATGTTTG GGCAGGTGGC AGCTAGCAGC 
ACAGCCACTG CCACACCACA GGCCAGCAGC TCAGGGTTTG GCAGCCCTGC GTTTGGTGCT 
TCAGCTCCAG CAGTCTTTGG ACAGACAGCC TTTGGGCAGG CCCCGGCCTT TGGGCAAGCA 
GCAAGCAACC CAGCAAGTGG CTTCTCTTTC AGCCAGCCTG GGTTCAGCTC TGGGCCTGTT 
TTTGGCCAGT CTGTTTCCTC TACCCCCACA TCCACCAGTG CGAATGTCTT CGGAGCCGCC 
TCGAGTGCCA GCAGCCCTGG TTCCTTCTCA TTTGGACAGG CTTCTACCAA CACAGGAGGG 
ACACTGTTTG GCCAGAACAA CCCCCCTGCT TTTGGTCAAA GCCCTGGCTT TGGACAGGGA 
GGCTCTGTGT TTGGTAGCAC CTCGGCCACT ACCTCTACAG CAGCACCTTC AGGGTTTAGC 
TTTTGTCAAG CCTCTGGTTT TGGGTCTAGT AATACTGGTT CTGTGTTTGG TCAGGCATCC 
AGTACTGGCG GATCAGTCTT TGGCCAGCAG TCATCTACTT CCAGTGGTAG TGTGTTTGGG 
TCTGGAAACT CGACAAGAGG GAGCGGTTTC TTCAGTGGCC TTGGAGGAAA ACCCAGCCAG 
GATGCTGCCA ACAAAAACCC ATTCAGCTCA GCCAGTGGGG GCTTTGGATC TACAACCACC 
CCAAATACCG CTAATCTATT TGGAAACAGT GGCGCTAAGA CATTTGGAGG GTTTGGCAGC 
TCATCCTTTG GGGAACAGAA GCCTGCTGGC ACTTTCAGCT CTGGTGGTGG AAGCGTGGCA 
TCCCAAGGCT TTGGATTTTC TACTCCAAAT AAAACAGGTG GCTTTGGTGC TGCTCCAGTG 
TTTGGCAGCC CTCCTACTTT TGGGGGATCC CCTGGGTTTG GAGGGGTGCC AGCATTCGGT 
TCAGCCCCGG CCTTTACAAG CCCTCTGGGC TCGACGGGAG GCAAAGTGTT CGGAGAGGGT 
ACTGCGGCTG CCAGCGCAGG AGGATTCGGG TTTGGGAGCA GCAGCAGCAC TGCATCCTTT 
GGTACCCTCG CCAGTCAGAA CGCGCCAACC TTCGGGTCCT TGTCCCAGCA GACCTCAGGC 
TTTGGGACCC AGAACAGTGG CTTCTCTGGG TTTGGATCGG GCACAGGAGC GTTCGCCTTT 
GGATCATCGA ACTCGTCTGT CCAAGGCTTT GGTGGCTGGA GAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup214 ( XM_013126431.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012981885.1 Mesocricetus auratus nucleoporin 214 (Nup214), transcript variant X1, mRNA.

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CloneID	OMe142310
Clone ID Related Accession (Same CDS sequence)	XM_013126431.1 , XM_013126431.2
Accession Version	XM_013126431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	nuclear pore complex protein Nup214 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801898.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_013126431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACG ATATGGATGC CATAGTCCCT GAGCGGGAGA TGAAGGACTT TCAGTTTAGA 
GCACTGAAGA AAGTAAGGAT CTTTGACTCT CCTGAAGAGT TGCCCAAGGC GCGCTCGAGT 
ATGCTTGCCA TATCCAATAA GTATGGTATG CTCTTTGCTG GTGGCGCCGG TGGCTTGCAT 
GTTTTTCCTA CTAAAAGTCT TCTTATCCAA AACAGACCTG GAGATGATCC CAATAAAATT 
GTTGAGCCAG TCGACAGCTT GAATGTTGCT CTGAAATTCC CAGTACATCA CCTGGCACTG 
AGCTGTGACA ACCTCACACT CTCTGCATGC ATGGTGTCCA GTGAATACGG CTCCGTTATA 
GCTTTTTTTG ATGTTCGCAC ATTTTCGAAT CAGGCTAAAC CAGTGAAACT CCCATTTACC 
TATCACAAGC TTTCAAAGGA TGTAGGTGGT ATGGTGACTG ATATGAAGTG GAACCCCACT 
GTCCCGTCCA TGGTGGCAGT TTGTCTGGCT GATGGCAGTA TTGCTGTCCT GCAAGTTACA 
GAAGTGGTGA AAGTGTGTGC CACTCTTCCT CCCTCGGCAG CAGTTACATG TGTGTGCTGG 
AGTCCCAAAG GAAAGCAGCT GGCAGTAGGG AAGCAGAATG GAACCGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCA TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTAAGAGTCC TGGATGTGCT GTGGATTGGC ACCTATGTCT TCATGATAGT TTATGCTGGT 
GCAGATGTGA CCATGGAAAC CTGTCCAGAT GTGGTGATGG CTTTACTCCC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GCTACAGCAG CTGCACAGAG 
CGACAGCATC ATTACTACCT CAATTACATC GAGGAGTGGG ATTTGGTGCT GGCAGCATCT 
GCAGCGTCAA CAGAAGTTAG CATCCTTGCT CGACAAAGTG ATCAGACTAA TTGGGAATCT 
TGGCTGCTGG AGGACTCTAG TCGAGCTGAA CTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GCGTTGCCAT AGATTATACT AATGAAGTAG AAGTCGTCAT CAACGATGAA 
AAGACCCTTC CTGCTGCTCC GGTTCTCCTG TTACTTTCAA CAGATGGTGT GCTTTGTCCG 
TTTTATATGA TCAATCAAAA TCCTGGGGTT AGGTCCCTAA TCAAGACCAT GGAGCTGGCC 
ACAATAGAAG GAGAGCGACA GCCCAAGTCC TCAGAAAGTT TTCCTACCAC TCCAACCTCC 
CCTCAAGCCC CGGAGAACCT TGATGTCCCA GAAGAAGCCT CTTTGTCCCT TGCACCGGTG 
TCCTCTGCTG TTCCTACCAC CACCTTCCCT TTGCCCTCTT CTGGGGGGGC CCCTTGTGTG 
TTCTCCTTTG GCCCCTCATC CTTAAAGCCA TCTGTGTCTG TGGCTGGGGA GCCTCCTCTG 
GTTCCCTCTG GCTCCGACAA TCCCAGAGCA GCTCTGGGCT CTGGCACCTC CACCTTCCCC 
TTTGCCCCTC CTTCCAAAGG CCCCCAGGCC CTTAACCCTG CAGCACCCTC CATGGCCACA 
CCTGCTGCTC CCTTCACTTT TGGATCATCA GGTTTTAAGC CCACCTTGGA AAGCACACCA 
ATGTCCAGTG CTCCAAACAC AGGGATGAAG CCTTCGTTCC CACCCTCAGC CTCTTCTGTG 
AAAGTCAACC TGAATGAAAA GTTCACCGCT GTGGCCTCTG CGCCTGTCCA TAGCTCCACG 
AGCACACCCT CCCTGTTGCC ATTCTCTTCC TCCCCCAAGC CAGCTGCTTC TGGACAGCTC 
AGCCATCCCA CGCCTCTGCC GGCATCGTCC AGCTCCATTC CTTTGAAGTC TTCAGTCTCT 
CCCTCGCCAT CAGGCCGATC TACTCCGAGT GTTCCAAGCT CAGTTCCCTC CATGGTACAG 
AAATCACCCA GGATAACCCC TCCAGTGGTA AAGTCAGGCT CTCCTCAGGC TAAGTCCCTT 
CAGCCTCCTG TCACAGAGAA ACAGGGACAT CAGTGGAAGG ATTCAGACCC TGTGATGGCT 
GGCATTGGAG AAGAGATTGC ACATTTCCAG AAAGAGCTAG AGGAGTTAAA GACCCGGACC 
TCCAAAGCCT GTTTCCAGGT TGGCACCTTG GAGGAGATGA AGACCCTGCG GACAGAATCT 
GATGACTTGC ACACCTTCCT CTTCGAGATT AGAGAGGCCA CAGAGTCTCT TCATGGAGAC 
ATAAGTACCC TGAAAACAAC TCTGCTGGAG GGCTTTGCTG GTGTTGAAGA AGCCAGAGAA 
CAAAACGGAA GAAACCATGA CTCTGGGTAT CTGCACCTGC TTTATAAAAG ACCATTGGAT 
CCCAAGAGTG AAGCTCAGCT CCAGGAAATC CGTCGTCTTC ATCAGTATGT GAAGTTTGCT 
GTCCAGGATG TAAATGATGT TTTAGACCTG GAGTGGGATC GGCATCTGGA ACAGAAGAAA 
AGACAGAGGC GTTTGCTTGT GCCAGAACGA GAGACACTTT TCAACACACT AGCCAACAAC 
CGGGAGATCA TCAACCAACA GAGGAAGAGG TTGAATCACC TGGTGGACAG TCTGCAGCAG 
CTCCGTCTCT ATAACCAGAC CCCCCCATGG AATCTGCCCT CAACACTCTC AACTCAGAGC 
AGCACTCACA GTTTTGACAG TGACCTTGAA AGCCTGCTAA AAACAACCGT TGAATCTCAC 
ACCAAGCCCT CTTCCAGAGT GCAAGCTAAA CTGTCCCCCG CTAAACAGGC CCAACTGAGG 
AACTTCTTAG CCAAAAGGAA GACCCCACCA GTGAGGTCCA CTGCTCCAGC CAGCCTGTCT 
CGGTCAGCCT TCCTGTCTCA GAGATATTAT GAAGACTTAG ATGAAGCCAT CTCCACATCC 
TCCATCTGTC AGCCCCTGGA GGGAGAAGAT GCCCGGTCTC CCTGCAAAGA GGAGGAAGCT 
GTGGTCCAGG TCCATAGGCA TGCTCCTGTG GTCCGCACGC CTTCTATCCA ACCCAGTCTC 
CTGCCCCAGG CAATGCCTTT TGCTAAGCCT CATCTGTTTC ATAGTTCTTC ACCTGCTGTG 
ATGAGCTCCT CAGTCTCTAC ATCAGCCAGC AAAGTTATTC CTCCAGGAGC CGACAGCACG 
ATGCTGGCAA CCAAAACTGT TAAGCATGGT GCACCAGGTC CTTCCCACAC TGTCGCAGCT 
TCCCAGGCGG CAGCAGCTGC AGCCATGAGA CGGCAGATGG CCAATCAGGT GCCAGCTGTG 
AGCACTCTCA CCGAATCAAC TTTGAAGAAT GTTCCTCAAG TGGTAAACGT ACAGGAACTG 
AAGAGTAGCC CTGCAGCCCC GTCTGTGGCC CTGGGTTCTT CAGTGCCGCA CTCAACAGCC 
AAAGCACCCC ATGCAGTGTT GGCCCCGGTG GCTGCCAGCC AAGCTAAGCA GGGCTCTCTG 
ATAAATTCCT TCAAGCCGTC AGGGACCACA CCAGCATCCT GTCAATTGTC CTCTGGAGAT 
AAAGCTGCAG GTTCAGGCAC AGCCAAGACG GAATCAACCG TGACCTCGAC TCCATCTGCT 
GTGGGGCAGC CCAACAAACC TTTCTCATTT GCTCCCTCAG GGACTGCCTT TACTTTTGGG 
ACGATCGCAT CGGCGCCCTC TTCTAGTCTC GCTGCCACAC CAGGATCAGT ACCCCCCACG 
AAAGAGTCAA ACCAGCTGGA GGCATTTTCA TTTGGCGGGG GAGGCAAACC TTTCTGTGAG 
GCCATTCCTG GAAGCTCTCC TCCCACAGGA GCCTTGTCAG CAGCACCCAG CACTTCTGTA 
ACGACTGCCT CTCCAGGAGA CTCTGTCCCA CCCGGCAGCA GACCTGCAGC TTCCTCTGGA 
ATTACTGTGT CCACTGCCTC TAGCAAATTG GAAACTCCAA CGTCTAAGCT GGGAGAGTTC 
CTGTTCCCAG GCTCCCTGAC TGGAGAAACG CTAGGGAGCT TTTCAGGACT GCGTGTTGGC 
CAAGCTGAGG ACTCTGCAAA GCCAGTCAGT AAGGCCTCAT CCATGAGCCT AGCTGGATCC 
CCGACACCAA AGCCCCCAGG CTTTAGCTTT CCCGGCACTC CAGTGTTAGG GAAGCCTGTG 
GAGCCCACTG TGACATCCTC TGTAACCCCT GCCCCGGCAG CGCCAGTGTC TTCCCTGGGT 
GTGAGCACTA ACAGTTCGGC TGCGCCAGTC TTTGCCAGTC TGCCCCTTAG CAGTGCCGGA 
CCTTCTGGGC TCATCAGTTT TGGTGGGGCA TCTCTGGCCG GTAGCAAAGC TGGTTTCGCA 
TTTGGAAACC AACAGACTAG TAGTACTGTG TCATCAGCTG CCCCAACATC CACCTCAGTT 
GCCCCTTCCC TTCCAGCGTC TTTCCCCACC TTATCATTTG GTGGCCTCCT AAGTTCAACG 
TCTACTTCTT CCCTTCCTGT GTCCTCTGGT AAGAGCCCAG AGGAGGCTAC ACCATCAGCT 
GTGCCTGATA AGTCAGGCCA CAGTGAAGTC TCAGCATCAG CACCCTCCCT TCCAGTACAG 
CCACAATCCG CCCAGCCTCC CCAGGCTTCT CTGCAAACTT CTGACCCTGT CAAAACAGAG 
CCTGTTCTTG TGCAGGCCAC AGACAGCAGC CCCAACCCAC CATCATCTGC AGCCAGTGTT 
GTGGCATCTA CAGAGGCCAC TCCAGTAGCC CCAGGGGGCC CTGACAGCAA GACAGAGGCT 
ACTGCTCCTG CGTCTACCTT TGCGGGGCCT GGGCAGGCTG CTGTCGCAGC GGCTGTTCTC 
CCAGGGACAG GCCCAGCAGC CAGTGAAGCA TCGGGTACTC CTAGCACCTC CAGCACAGCT 
TCCAGCAGCG TCCCAGCTTC TGCCACAGAG ACAGCCGCGT TTGGGACTGC TACTTCTGGC 
TCCTCGGTCT GCACACAGCC ACCTGCTGCC AGTTCTAGCT CAGCGTTCAG CCAACTCTCC 
AGTAACACAG CCACTGCATC CTCTGCCACC CCCATGTTTG GGCAGGTGGC AGCTAGCAGC 
ACAGCCACTG CCACACCACA GGCCAGCAGC TCAGGGTTTG GCAGCCCTGC GTTTGGTGCT 
TCAGCTCCAG CAGTCTTTGG ACAGACAGCC TTTGGGCAGG CCCCGGCCTT TGGGCAAGCA 
GCAAGCAACC CAGCAAGTGG CTTCTCTTTC AGCCAGCCTG GGTTCAGCTC TGGGCCTGTT 
TTTGGCCAGT CTGTTTCCTC TACCCCCACA TCCACCAGTG CGAATGTCTT CGGAGCCGCC 
TCGAGTGCCA GCAGCCCTGG TTCCTTCTCA TTTGGACAGG CTTCTACCAA CACAGGAGGG 
ACACTGTTTG GCCAGAACAA CCCCCCTGCT TTTGGTCAAA GCCCTGGCTT TGGACAGGGA 
GGCTCTGTGT TTGGTAGCAC CTCGGCCACT ACCTCTACAG CAGCACCTTC AGGGTTTAGC 
TTTTGTCAAG CCTCTGGTTT TGGGTCTAGT AATACTGGTT CTGTGTTTGG TCAGGCATCC 
AGTACTGGCG GATCAGTCTT TGGCCAGCAG TCATCTACTT CCAGTGGTAG TGTGTTTGGG 
TCTGGAAACT CGACAAGAGG GAGCGGTTTC TTCAGTGGCC TTGGAGGAAA ACCCAGCCAG 
GATGCTGCCA ACAAAAACCC ATTCAGCTCA GCCAGTGGGG GCTTTGGATC TACAACCACC 
CCAAATACCG CTAATCTATT TGGAAACAGT GGCGCTAAGA CATTTGGAGG GTTTGGCAGC 
TCATCCTTTG GGGAACAGAA GCCTGCTGGC ACTTTCAGCT CTGGTGGTGG AAGCGTGGCA 
TCCCAAGGCT TTGGATTTTC TACTCCAAAT AAAACAGGTG GCTTTGGTGC TGCTCCAGTG 
TTTGGCAGCC CTCCTACTTT TGGGGGATCC CCTGGGTTTG GAGGGGTGCC AGCATTCGGT 
TCAGCCCCGG CCTTTACAAG CCCTCTGGGC TCGACGGGAG GCAAAGTGTT CGGAGAGGGT 
ACTGCGGCTG CCAGCGCAGG AGGATTCGGG TTTGGGAGCA GCAGCAGCAC TGCATCCTTT 
GGTACCCTCG CCAGTCAGAA CGCGCCAACC TTCGGGTCCT TGTCCCAGCA GACCTCAGGC 
TTTGGGACCC AGAACAGTGG CTTCTCTGGG TTTGGATCGG GCACAGGAGC GTTCGCCTTT 
GGATCATCGA ACTCGTCTGT CCAAGGCTTT GGTGGCTGGA GAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012981885.1
CDS	122..6349
Translation	MGDDMDAIVPEREMKDFQFRALKKVRIFDSPEELPKARSSMLAISNKYGMLFAGGAGGLHVFPTKSLLIQNRPGDDPNKIVEPVDSLNVALKFPVHHLALSCDNLTLSACMVSSEYGSVIAFFDVRTFSNQAKPVKLPFTYHKLSKDVGGMVTDMKWNPTVPSMVAVCLADGSIAVLQVTEVVKVCATLPPSAAVTCVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFMIVYAGADVTMETCPDVVMALLPKKEEKHPEIFVNFMEPCYSSCTERQHHYYLNYIEEWDLVLAASAASTEVSILARQSDQTNWESWLLEDSSRAELPVTDKSDDSLPMGVAIDYTNEVEVVINDEKTLPAAPVLLLLSTDGVLCPFYMINQNPGVRSLIKTMELATIEGERQPKSSESFPTTPTSPQAPENLDVPEEASLSLAPVSSAVPTTTFPLPSSGGAPCVFSFGPSSLKPSVSVAGEPPLVPSGSDNPRAALGSGTSTFPFAPPSKGPQALNPAAPSMATPAAPFTFGSSGFKPTLESTPMSSAPNTGMKPSFPPSASSVKVNLNEKFTAVASAPVHSSTSTPSLLPFSSSPKPAASGQLSHPTPLPASSSSIPLKSSVSPSPSGRSTPSVPSSVPSMVQKSPRITPPVVKSGSPQAKSLQPPVTEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKTRTSKACFQVGTLEEMKTLRTESDDLHTFLFEIREATESLHGDISTLKTTLLEGFAGVEEAREQNGRNHDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKKRQRRLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYNQTPPWNLPSTLSTQSSTHSFDSDLESLLKTTVESHTKPSSRVQAKLSPAKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEAISTSSICQPLEGEDARSPCKEEEAVVQVHRHAPVVRTPSIQPSLLPQAMPFAKPHLFHSSSPAVMSSSVSTSASKVIPPGADSTMLATKTVKHGAPGPSHTVAASQAAAAAAMRRQMANQVPAVSTLTESTLKNVPQVVNVQELKSSPAAPSVALGSSVPHSTAKAPHAVLAPVAASQAKQGSLINSFKPSGTTPASCQLSSGDKAAGSGTAKTESTVTSTPSAVGQPNKPFSFAPSGTAFTFGTIASAPSSSLAATPGSVPPTKESNQLEAFSFGGGGKPFCEAIPGSSPPTGALSAAPSTSVTTASPGDSVPPGSRPAASSGITVSTASSKLETPTSKLGEFLFPGSLTGETLGSFSGLRVGQAEDSAKPVSKASSMSLAGSPTPKPPGFSFPGTPVLGKPVEPTVTSSVTPAPAAPVSSLGVSTNSSAAPVFASLPLSSAGPSGLISFGGASLAGSKAGFAFGNQQTSSTVSSAAPTSTSVAPSLPASFPTLSFGGLLSSTSTSSLPVSSGKSPEEATPSAVPDKSGHSEVSASAPSLPVQPQSAQPPQASLQTSDPVKTEPVLVQATDSSPNPPSSAASVVASTEATPVAPGGPDSKTEATAPASTFAGPGQAAVAAAVLPGTGPAASEASGTPSTSSTASSSVPASATETAAFGTATSGSSVCTQPPAASSSSAFSQLSSNTATASSATPMFGQVAASSTATATPQASSSGFGSPAFGASAPAVFGQTAFGQAPAFGQAASNPASGFSFSQPGFSSGPVFGQSVSSTPTSTSANVFGAASSASSPGSFSFGQASTNTGGTLFGQNNPPAFGQSPGFGQGGSVFGSTSATTSTAAPSGFSFCQASGFGSSNTGSVFGQASSTGGSVFGQQSSTSSGSVFGSGNSTRGSGFFSGLGGKPSQDAANKNPFSSASGGFGSTTTPNTANLFGNSGAKTFGGFGSSSFGEQKPAGTFSSGGGSVASQGFGFSTPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSSTASFGTLASQNAPTFGSLSQQTSGFGTQNSGFSGFGSGTGAFAFGSSNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_013126431.2
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus nucleoporin 214 (Nup214), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013126431.2

ORF Insert Sequence:

ATGGGAGACG ATATGGATGC CATAGTCCCT GAGCGGGAGA TGAAGGACTT TCAGTTTAGA 
GCACTGAAGA AAGTAAGGAT CTTTGACTCT CCTGAAGAGT TGCCCAAGGC GCGCTCGAGT 
ATGCTTGCCA TATCCAATAA GTATGGTATG CTCTTTGCTG GTGGCGCCGG TGGCTTGCAT 
GTTTTTCCTA CTAAAAGTCT TCTTATCCAA AACAGACCTG GAGATGATCC CAATAAAATT 
GTTGAGCCAG TCGACAGCTT GAATGTTGCT CTGAAATTCC CAGTACATCA CCTGGCACTG 
AGCTGTGACA ACCTCACACT CTCTGCATGC ATGGTGTCCA GTGAATACGG CTCCGTTATA 
GCTTTTTTTG ATGTTCGCAC ATTTTCGAAT CAGGCTAAAC CAGTGAAACT CCCATTTACC 
TATCACAAGC TTTCAAAGGA TGTAGGTGGT ATGGTGACTG ATATGAAGTG GAACCCCACT 
GTCCCGTCCA TGGTGGCAGT TTGTCTGGCT GATGGCAGTA TTGCTGTCCT GCAAGTTACA 
GAAGTGGTGA AAGTGTGTGC CACTCTTCCT CCCTCGGCAG CAGTTACATG TGTGTGCTGG 
AGTCCCAAAG GAAAGCAGCT GGCAGTAGGG AAGCAGAATG GAACCGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCA TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTAAGAGTCC TGGATGTGCT GTGGATTGGC ACCTATGTCT TCATGATAGT TTATGCTGGT 
GCAGATGTGA CCATGGAAAC CTGTCCAGAT GTGGTGATGG CTTTACTCCC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GCTACAGCAG CTGCACAGAG 
CGACAGCATC ATTACTACCT CAATTACATC GAGGAGTGGG ATTTGGTGCT GGCAGCATCT 
GCAGCGTCAA CAGAAGTTAG CATCCTTGCT CGACAAAGTG ATCAGACTAA TTGGGAATCT 
TGGCTGCTGG AGGACTCTAG TCGAGCTGAA CTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GCGTTGCCAT AGATTATACT AATGAAGTAG AAGTCGTCAT CAACGATGAA 
AAGACCCTTC CTGCTGCTCC GGTTCTCCTG TTACTTTCAA CAGATGGTGT GCTTTGTCCG 
TTTTATATGA TCAATCAAAA TCCTGGGGTT AGGTCCCTAA TCAAGACCAT GGAGCTGGCC 
ACAATAGAAG GAGAGCGACA GCCCAAGTCC TCAGAAAGTT TTCCTACCAC TCCAACCTCC 
CCTCAAGCCC CGGAGAACCT TGATGTCCCA GAAGAAGCCT CTTTGTCCCT TGCACCGGTG 
TCCTCTGCTG TTCCTACCAC CACCTTCCCT TTGCCCTCTT CTGGGGGGGC CCCTTGTGTG 
TTCTCCTTTG GCCCCTCATC CTTAAAGCCA TCTGTGTCTG TGGCTGGGGA GCCTCCTCTG 
GTTCCCTCTG GCTCCGACAA TCCCAGAGCA GCTCTGGGCT CTGGCACCTC CACCTTCCCC 
TTTGCCCCTC CTTCCAAAGG CCCCCAGGCC CTTAACCCTG CAGCACCCTC CATGGCCACA 
CCTGCTGCTC CCTTCACTTT TGGATCATCA GGTTTTAAGC CCACCTTGGA AAGCACACCA 
ATGTCCAGTG CTCCAAACAC AGGGATGAAG CCTTCGTTCC CACCCTCAGC CTCTTCTGTG 
AAAGTCAACC TGAATGAAAA GTTCACCGCT GTGGCCTCTG CGCCTGTCCA TAGCTCCACG 
AGCACACCCT CCCTGTTGCC ATTCTCTTCC TCCCCCAAGC CAGCTGCTTC TGGACAGCTC 
AGCCATCCCA CGCCTCTGCC GGCATCGTCC AGCTCCATTC CTTTGAAGTC TTCAGTCTCT 
CCCTCGCCAT CAGGCCGATC TACTCCGAGT GTTCCAAGCT CAGTTCCCTC CATGGTACAG 
AAATCACCCA GGATAACCCC TCCAGTGGTA AAGTCAGGCT CTCCTCAGGC TAAGTCCCTT 
CAGCCTCCTG TCACAGAGAA ACAGGGACAT CAGTGGAAGG ATTCAGACCC TGTGATGGCT 
GGCATTGGAG AAGAGATTGC ACATTTCCAG AAAGAGCTAG AGGAGTTAAA GACCCGGACC 
TCCAAAGCCT GTTTCCAGGT TGGCACCTTG GAGGAGATGA AGACCCTGCG GACAGAATCT 
GATGACTTGC ACACCTTCCT CTTCGAGATT AGAGAGGCCA CAGAGTCTCT TCATGGAGAC 
ATAAGTACCC TGAAAACAAC TCTGCTGGAG GGCTTTGCTG GTGTTGAAGA AGCCAGAGAA 
CAAAACGGAA GAAACCATGA CTCTGGGTAT CTGCACCTGC TTTATAAAAG ACCATTGGAT 
CCCAAGAGTG AAGCTCAGCT CCAGGAAATC CGTCGTCTTC ATCAGTATGT GAAGTTTGCT 
GTCCAGGATG TAAATGATGT TTTAGACCTG GAGTGGGATC GGCATCTGGA ACAGAAGAAA 
AGACAGAGGC GTTTGCTTGT GCCAGAACGA GAGACACTTT TCAACACACT AGCCAACAAC 
CGGGAGATCA TCAACCAACA GAGGAAGAGG TTGAATCACC TGGTGGACAG TCTGCAGCAG 
CTCCGTCTCT ATAACCAGAC CCCCCCATGG AATCTGCCCT CAACACTCTC AACTCAGAGC 
AGCACTCACA GTTTTGACAG TGACCTTGAA AGCCTGCTAA AAACAACCGT TGAATCTCAC 
ACCAAGCCCT CTTCCAGAGT GCAAGCTAAA CTGTCCCCCG CTAAACAGGC CCAACTGAGG 
AACTTCTTAG CCAAAAGGAA GACCCCACCA GTGAGGTCCA CTGCTCCAGC CAGCCTGTCT 
CGGTCAGCCT TCCTGTCTCA GAGATATTAT GAAGACTTAG ATGAAGCCAT CTCCACATCC 
TCCATCTGTC AGCCCCTGGA GGGAGAAGAT GCCCGGTCTC CCTGCAAAGA GGAGGAAGCT 
GTGGTCCAGG TCCATAGGCA TGCTCCTGTG GTCCGCACGC CTTCTATCCA ACCCAGTCTC 
CTGCCCCAGG CAATGCCTTT TGCTAAGCCT CATCTGTTTC ATAGTTCTTC ACCTGCTGTG 
ATGAGCTCCT CAGTCTCTAC ATCAGCCAGC AAAGTTATTC CTCCAGGAGC CGACAGCACG 
ATGCTGGCAA CCAAAACTGT TAAGCATGGT GCACCAGGTC CTTCCCACAC TGTCGCAGCT 
TCCCAGGCGG CAGCAGCTGC AGCCATGAGA CGGCAGATGG CCAATCAGGT GCCAGCTGTG 
AGCACTCTCA CCGAATCAAC TTTGAAGAAT GTTCCTCAAG TGGTAAACGT ACAGGAACTG 
AAGAGTAGCC CTGCAGCCCC GTCTGTGGCC CTGGGTTCTT CAGTGCCGCA CTCAACAGCC 
AAAGCACCCC ATGCAGTGTT GGCCCCGGTG GCTGCCAGCC AAGCTAAGCA GGGCTCTCTG 
ATAAATTCCT TCAAGCCGTC AGGGACCACA CCAGCATCCT GTCAATTGTC CTCTGGAGAT 
AAAGCTGCAG GTTCAGGCAC AGCCAAGACG GAATCAACCG TGACCTCGAC TCCATCTGCT 
GTGGGGCAGC CCAACAAACC TTTCTCATTT GCTCCCTCAG GGACTGCCTT TACTTTTGGG 
ACGATCGCAT CGGCGCCCTC TTCTAGTCTC GCTGCCACAC CAGGATCAGT ACCCCCCACG 
AAAGAGTCAA ACCAGCTGGA GGCATTTTCA TTTGGCGGGG GAGGCAAACC TTTCTGTGAG 
GCCATTCCTG GAAGCTCTCC TCCCACAGGA GCCTTGTCAG CAGCACCCAG CACTTCTGTA 
ACGACTGCCT CTCCAGGAGA CTCTGTCCCA CCCGGCAGCA GACCTGCAGC TTCCTCTGGA 
ATTACTGTGT CCACTGCCTC TAGCAAATTG GAAACTCCAA CGTCTAAGCT GGGAGAGTTC 
CTGTTCCCAG GCTCCCTGAC TGGAGAAACG CTAGGGAGCT TTTCAGGACT GCGTGTTGGC 
CAAGCTGAGG ACTCTGCAAA GCCAGTCAGT AAGGCCTCAT CCATGAGCCT AGCTGGATCC 
CCGACACCAA AGCCCCCAGG CTTTAGCTTT CCCGGCACTC CAGTGTTAGG GAAGCCTGTG 
GAGCCCACTG TGACATCCTC TGTAACCCCT GCCCCGGCAG CGCCAGTGTC TTCCCTGGGT 
GTGAGCACTA ACAGTTCGGC TGCGCCAGTC TTTGCCAGTC TGCCCCTTAG CAGTGCCGGA 
CCTTCTGGGC TCATCAGTTT TGGTGGGGCA TCTCTGGCCG GTAGCAAAGC TGGTTTCGCA 
TTTGGAAACC AACAGACTAG TAGTACTGTG TCATCAGCTG CCCCAACATC CACCTCAGTT 
GCCCCTTCCC TTCCAGCGTC TTTCCCCACC TTATCATTTG GTGGCCTCCT AAGTTCAACG 
TCTACTTCTT CCCTTCCTGT GTCCTCTGGT AAGAGCCCAG AGGAGGCTAC ACCATCAGCT 
GTGCCTGATA AGTCAGGCCA CAGTGAAGTC TCAGCATCAG CACCCTCCCT TCCAGTACAG 
CCACAATCCG CCCAGCCTCC CCAGGCTTCT CTGCAAACTT CTGACCCTGT CAAAACAGAG 
CCTGTTCTTG TGCAGGCCAC AGACAGCAGC CCCAACCCAC CATCATCTGC AGCCAGTGTT 
GTGGCATCTA CAGAGGCCAC TCCAGTAGCC CCAGGGGGCC CTGACAGCAA GACAGAGGCT 
ACTGCTCCTG CGTCTACCTT TGCGGGGCCT GGGCAGGCTG CTGTCGCAGC GGCTGTTCTC 
CCAGGGACAG GCCCAGCAGC CAGTGAAGCA TCGGGTACTC CTAGCACCTC CAGCACAGCT 
TCCAGCAGCG TCCCAGCTTC TGCCACAGAG ACAGCCGCGT TTGGGACTGC TACTTCTGGC 
TCCTCGGTCT GCACACAGCC ACCTGCTGCC AGTTCTAGCT CAGCGTTCAG CCAACTCTCC 
AGTAACACAG CCACTGCATC CTCTGCCACC CCCATGTTTG GGCAGGTGGC AGCTAGCAGC 
ACAGCCACTG CCACACCACA GGCCAGCAGC TCAGGGTTTG GCAGCCCTGC GTTTGGTGCT 
TCAGCTCCAG CAGTCTTTGG ACAGACAGCC TTTGGGCAGG CCCCGGCCTT TGGGCAAGCA 
GCAAGCAACC CAGCAAGTGG CTTCTCTTTC AGCCAGCCTG GGTTCAGCTC TGGGCCTGTT 
TTTGGCCAGT CTGTTTCCTC TACCCCCACA TCCACCAGTG CGAATGTCTT CGGAGCCGCC 
TCGAGTGCCA GCAGCCCTGG TTCCTTCTCA TTTGGACAGG CTTCTACCAA CACAGGAGGG 
ACACTGTTTG GCCAGAACAA CCCCCCTGCT TTTGGTCAAA GCCCTGGCTT TGGACAGGGA 
GGCTCTGTGT TTGGTAGCAC CTCGGCCACT ACCTCTACAG CAGCACCTTC AGGGTTTAGC 
TTTTGTCAAG CCTCTGGTTT TGGGTCTAGT AATACTGGTT CTGTGTTTGG TCAGGCATCC 
AGTACTGGCG GATCAGTCTT TGGCCAGCAG TCATCTACTT CCAGTGGTAG TGTGTTTGGG 
TCTGGAAACT CGACAAGAGG GAGCGGTTTC TTCAGTGGCC TTGGAGGAAA ACCCAGCCAG 
GATGCTGCCA ACAAAAACCC ATTCAGCTCA GCCAGTGGGG GCTTTGGATC TACAACCACC 
CCAAATACCG CTAATCTATT TGGAAACAGT GGCGCTAAGA CATTTGGAGG GTTTGGCAGC 
TCATCCTTTG GGGAACAGAA GCCTGCTGGC ACTTTCAGCT CTGGTGGTGG AAGCGTGGCA 
TCCCAAGGCT TTGGATTTTC TACTCCAAAT AAAACAGGTG GCTTTGGTGC TGCTCCAGTG 
TTTGGCAGCC CTCCTACTTT TGGGGGATCC CCTGGGTTTG GAGGGGTGCC AGCATTCGGT 
TCAGCCCCGG CCTTTACAAG CCCTCTGGGC TCGACGGGAG GCAAAGTGTT CGGAGAGGGT 
ACTGCGGCTG CCAGCGCAGG AGGATTCGGG TTTGGGAGCA GCAGCAGCAC TGCATCCTTT 
GGTACCCTCG CCAGTCAGAA CGCGCCAACC TTCGGGTCCT TGTCCCAGCA GACCTCAGGC 
TTTGGGACCC AGAACAGTGG CTTCTCTGGG TTTGGATCGG GCACAGGAGC GTTCGCCTTT 
GGATCATCGA ACTCGTCTGT CCAAGGCTTT GGTGGCTGGA GAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup214 ( XM_021223885.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021079544.1 Mesocricetus auratus nucleoporin 214 (Nup214), transcript variant X2, mRNA.

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CloneID	OMe255878
Clone ID Related Accession (Same CDS sequence)	XM_021223885.1
Accession Version	XM_021223885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	nuclear pore complex protein Nup214 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801898.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACG ATATGGATGC CATAGTCCCT GAGCGGGAGA TGAAGGACTT TCAGTTTAGA 
GCACTGAAGA AAGTAAGGAT CTTTGACTCT CCTGAAGAGT TGCCCAAGGC GCGCTCGAGT 
ATGCTTGCCA TATCCAATAA GTATGGTATG CTCTTTGCTG GTGGCGCCGG TGGCTTGCAT 
GTTTTTCCTA CTAAAAGTCT TCTTATCCAA AACAGACCTG GAGATGATCC CAATAAAATT 
GTTGAGCCAG TCGACAGCTT GAATGTTGCT CTGAAATTCC CAGTACATCA CCTGGCACTG 
AGCTGTGACA ACCTCACACT CTCTGCATGC ATGGTGTCCA GTGAATACGG CTCCGTTATA 
GCTTTTTTTG ATGTTCGCAC ATTTTCGAAT CAGGCTAAAC CAGTGAAACT CCCATTTACC 
TATCACAAGC TTTCAAAGGA TGTAGGTGGT ATGGTGACTG ATATGAAGTG GAACCCCACT 
GTCCCGTCCA TGGTGGCAGT TTGTCTGGCT GATGGCAGTA TTGCTGTCCT GCAAGTTACA 
GAAGTGGTGA AAGTGTGTGC CACTCTTCCT CCCTCGGCAG CAGTTACATG TGTGTGCTGG 
AGTCCCAAAG GAAAGCAGCT GGCAGTAGGG AAGCAGAATG GAACCGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCA TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTAAGAGTCC TGGATGTGCT GTGGATTGGC ACCTATGTCT TCATGATAGT TTATGCTGGT 
GCAGATGTGA CCATGGAAAC CTGTCCAGAT GTGGTGATGG CTTTACTCCC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GCTACAGCAG CTGCACAGAG 
CGACAGCATC ATTACTACCT CAATTACATC GAGGAGTGGG ATTTGGTGCT GGCAGCATCT 
GCAGCGTCAA CAGAAGTTAG CATCCTTGCT CGACAAAGTG ATCAGACTAA TTGGGAATCT 
TGGCTGCTGG AGGACTCTAG TCGAGCTGAA CTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GCGTTGCCAT AGATTATACT AATGAAGTAG AAGTCGTCAT CAACGATGAA 
AAGACCCTTC CTGCTGCTCC GGTTCTCCTG TTACTTTCAA CAGATGGTGT GCTTTGTCCG 
TTTTATATGA TCAATCAAAA TCCTGGGGTT AGGTCCCTAA TCAAGACCAT GGAGCTGGCC 
ACAATAGAAG GAGAGCGACA GCCCAAGTCC TCAGAAAGTT TTCCTACCAC TCCAACCTCC 
CCTCAAGCCC CGGAGAACCT TGATGTCCCA GAAGAAGCCT CTTTGTCCCT TGCACCGGTG 
TCCTCTGCTG TTCCTACCAC CACCTTCCCT TTGCCCTCTT CTGGGGGGGC CCCTTGTGTG 
TTCTCCTTTG GCCCCTCATC CTTAAAGCCA TCTGTGTCTG TGGCTGGGGA GCCTCCTCTG 
GTTCCCTCTG GCTCCGACAA TCCCAGAGCA GCTCTGGGCT CTGGCACCTC CACCTTCCCC 
TTTGCCCCTC CTTCCAAAGG CCCCCAGGCC CTTAACCCTG CAGCACCCTC CATGGCCACA 
CCTGCTGCTC CCTTCACTTT TGGATCATCA GGTTTTAAGC CCACCTTGGA AAGCACACCA 
ATGTCCAGTG CTCCAAACAC AGGGATGAAG CCTTCGTTCC CACCCTCAGC CTCTTCTGTG 
AAAGTCAACC TGAATGAAAA GTTCACCGCT GTGGCCTCTG CGCCTGTCCA TAGCTCCACG 
AGCACACCCT CCCTGTTGCC ATTCTCTTCC TCCCCCAAGC CAGCTGCTTC TGGACAGCTC 
AGCCATCCCA CGCCTCTGCC GGCATCGTCC AGCTCCATTC CTTTGAAGTC TTCAGTCTCT 
CCCTCGCCAT CAGGCCGATC TACTCCGAGT GTTCCAAGCT CAGTTCCCTC CATGGTACAG 
AAATCACCCA GGATAACCCC TCCAGTGGTA AAGTCAGGCT CTCCTCAGGC TAAGTCCCTT 
CAGCCTCCTG TCACAGAGAA ACAGGGACAT CAGTGGAAGG ATTCAGACCC TGTGATGGCT 
GGCATTGGAG AAGAGATTGC ACATTTCCAG AAAGAGCTAG AGGAGTTAAA GACCCGGACC 
TCCAAAGCCT GTTTCCAGGT TGGCACCTTG GAGGAGATGA AGACCCTGCG GACAGAATCT 
GATGACTTGC ACACCTTCCT CTTCGAGATT AGAGAGGCCA CAGAGTCTCT TCATGGAGAC 
ATAAGTACCC TGAAAACAAC TCTGCTGGAG GGCTTTGCTG GTGTTGAAGA AGCCAGAGAA 
CAAAACGGAA GAAACCATGA CTCTGGGTAT CTGCACCTGC TTTATAAAAG ACCATTGGAT 
CCCAAGAGTG AAGCTCAGCT CCAGGAAATC CGTCGTCTTC ATCAGTATGT GAAGTTTGCT 
GTCCAGGATG TAAATGATGT TTTAGACCTG GAGTGGGATC GGCATCTGGA ACAGAAGAAA 
AGACAGAGGC GTTTGCTTGT GCCAGAACGA GAGACACTTT TCAACACACT AGCCAACAAC 
CGGGAGATCA TCAACCAACA GAGGAAGAGG TTGAATCACC TGGTGGACAG TCTGCAGCAG 
CTCCGTCTCT ATAACCAGAC CCCCCCATGG AATCTGCCCT CAACACTCTC AACTCAGAGC 
AGCACTCACA GTTTTGACAG TGACCTTGAA AGCCTGCTAA AAACAACCGT TGAATCTCAC 
ACCAAGCCCT CTTCCAGAGT GCAAGCTAAA CTGTCCCCCG CTAAACAGGC CCAACTGAGG 
AACTTCTTAG CCAAAAGGAA GACCCCACCA GTGAGGTCCA CTGCTCCAGC CAGCCTGTCT 
CGGTCAGCCT TCCTGTCTCA GAGATATTAT GAAGACTTAG ATGAAGCCAT CTCCACATCC 
TCCATCTGTC AGCCCCTGGA GGGAGAAGAT GCCCGGTCTC CCTGCAAAGA GGAGGAAGCT 
GTGGTCCAGG TCCATAGGCA TGCTCCTGTG GTCCGCACGC CTTCTATCCA ACCCAGTCTC 
CTGCCCCAGG CAATGCCTTT TGCTAAGCCT CATCTGTTTC ATAGTTCTTC ACCTGCTGTG 
ATGAGCTCCT CAGTCTCTAC ATCAGCCAGC AAAGTTATTC CTCCAGGAGC CGACAGCACG 
ATGCTGGCAA CCAAAACTGT TAAGCATGGT GCACCAGGTC CTTCCCACAC TGTCGCAGCT 
TCCCAGGCGG CAGCAGCTGC AGCCATGAGA CGGCAGATGG CCAATCAGGT GCCAGCTGTG 
AGCACTCTCA CCGAATCAAC TTTGAAGAAT GTTCCTCAAG TGGTAAACGT ACAGGAACTG 
AAGAGTAGCC CTGCAGCCCC GTCTGTGGCC CTGGGTTCTT CAGTGCCGCA CTCAACAGCC 
AAAGCACCCC ATGCAGTGTT GGCCCCGGTG GCTGCCAGCC AAGCTAAGCA GGGCTCTCTG 
ATAAATTCCT TCAAGCCGTC AGGGACCACA CCAGCATCCT GTCAATTGTC CTCTGGAGAT 
AAAGCTGCAG GTTCAGGCAC AGCCAAGACG GAATCAACCG TGACCTCGAC TCCATCTGCT 
GTGGGGCAGC CCAACAAACC TTTCTCATTT GCTCCCTCAG GGACTGCCTT TACTTTTGGG 
ACGATCGCAT CGGCGCCCTC TTCTAGTCTC GCTGCCACAC CAGGATCAGT ACCCCCCACG 
AAAGAGTCAA ACCAGCTGGA GGCATTTTCA TTTGGCGGGG GAGGCAAACC TTTCTGTGAG 
GCCATTCCTG GAAGCTCTCC TCCCACAGGA GCCTTGTCAG CAGCACCCAG CACTTCTGTA 
ACGACTGCCT CTCCAGGAGA CTCTGTCCCA CCCGGCAGCA GACCTGCAGC TTCCTCTGGA 
ATTACTGTGT CCACTGCCTC TAGCAAATTG GAAACTCCAA CGTCTAAGCT GGGAGAGTTC 
CTGTTCCCAG GCTCCCTGAC TGGAGAAACG CTAGGGAGCT TTTCAGGACT GCGTGTTGGC 
CAAGCTGAGG ACTCTGCAAA GCCAGTCAGT AAGGCCTCAT CCATGAGCCT AGCTGGATCC 
CCGACACCAA AGCCCCCAGG CTTTAGCTTT CCCGGCACTC CAGTGTTAGG GAAGCCTGTG 
GAGCCCACTG TGACATCCTC TGTAACCCCT GCCCCGGCAG CGCCAGTGTC TTCCCTGGGT 
GTGAGCACTA ACAGTTCGGC TGCGCCAGTC TTTGCCAGTC TGCCCCTTAG CAGTGCCGGA 
CCTTCTGGGC TCATCAGTTT TGGTGGGGCA TCTCTGGCCG GTAGCAAAGC TGGTTTCGCA 
TTTGGAAACC AACAGACTAG TAGTACTGTG TCATCAGCTG CCCCAACATC CACCTCAGTT 
GCCCCTTCCC TTCCAGCGTC TTTCCCCACC TTATCATTTG GTGGCCTCCT AAGTTCAACG 
TCTACTTCTT CCCTTCCTGT GTCCTCTGGT AAGAGCCCAG AGGAGGCTAC ACCATCAGCT 
GTGCCTGATA AGTCAGGCCA CAGTGAAGTC TCAGCATCAG CACCCTCCCT TCCAGTACAG 
CCACAATCCG CCCAGCCTCC CCAGGCTTCT CTGCAAACTT CTGACCCTGT CAAAACAGAG 
CCTGTTCTTG TGCAGGCCAC AGACAGCAGC CCCAACCCAC CATCATCTGC AGCCAGTGTT 
GTGGCATCTA CAGAGGCCAC TCCAGTAGCC CCAGGGGGCC CTGACAGCAA GACAGAGGCT 
ACTGCTCCTG CGTCTACCTT TGCGGGGCCT GGGCAGGCTG CTGTCGCAGC GGCTGTTCTC 
CCAGGGACAG GCCCAGCAGC CAGTGAAGCA TCGGGTACTC CTAGCACCTC CAGCACAGCT 
TCCAGCAGCG TCCCAGCTTC TGCCACAGAG ACAGCCGCGT TTGGGACTGC TACTTCTGGC 
TCCTCGGTCT GCACACAGCC ACCTGCTGCC AGTTCTAGCT CAGCGTTCAG CCAACTCTCC 
AGTAACACAG CCACTGCATC CTCTGCCACC CCCATGTTTG GGCAGGTGGC AGCTAGCAGC 
ACAGCCACTG CCACACCACA GGCCAGCAGC TCAGGGTTTG GCAGCCCTGC GTTTGGTGCT 
TCAGCTCCAG CAGTCTTTGG ACAGACAGCC TTTGGGCAGG CCCCGGCCTT TGGGCAAGCA 
GCAAGCAACC CAGCAAGTGG CTTCTCTTTC AGCCAGCCTG GGTTCAGCTC TGGGCCTGTT 
TTTGGCCAGT CTGTTTCCTC TACCCCCACA TCCACCAGTG CGAATGTCTT CGGAGCCGCC 
TCGAGTGCCA GCAGCCCTGG TTCCTTCTCA TTTGGACAGG CTTCTACCAA CACAGGAGGG 
ACACTGTTTG GCCAGAACAA CCCCCCTGCT TTTGGTCAAA GCCCTGGCTT TGGACAGGGA 
GGCTCTGTGT TTGGTAGCAC CTCGGCCACT ACCTCTACAG CAGCACCTTC AGGGTTTAGC 
TTTTGTCAAG CCTCTGGTTT TGGGTCTAGT AATACTGGTT CTGTGTTTGG TCAGGCATCC 
AGTACTGGCG GATCAGTCTT TGGCCAGTCA TCTACTTCCA GTGGTAGTGT GTTTGGGTCT 
GGAAACTCGA CAAGAGGGAG CGGTTTCTTC AGTGGCCTTG GAGGAAAACC CAGCCAGGAT 
GCTGCCAACA AAAACCCATT CAGCTCAGCC AGTGGGGGCT TTGGATCTAC AACCACCCCA 
AATACCGCTA ATCTATTTGG AAACAGTGGC GCTAAGACAT TTGGAGGGTT TGGCAGCTCA 
TCCTTTGGGG AACAGAAGCC TGCTGGCACT TTCAGCTCTG GTGGTGGAAG CGTGGCATCC 
CAAGGCTTTG GATTTTCTAC TCCAAATAAA ACAGGTGGCT TTGGTGCTGC TCCAGTGTTT 
GGCAGCCCTC CTACTTTTGG GGGATCCCCT GGGTTTGGAG GGGTGCCAGC ATTCGGTTCA 
GCCCCGGCCT TTACAAGCCC TCTGGGCTCG ACGGGAGGCA AAGTGTTCGG AGAGGGTACT 
GCGGCTGCCA GCGCAGGAGG ATTCGGGTTT GGGAGCAGCA GCAGCACTGC ATCCTTTGGT 
ACCCTCGCCA GTCAGAACGC GCCAACCTTC GGGTCCTTGT CCCAGCAGAC CTCAGGCTTT 
GGGACCCAGA ACAGTGGCTT CTCTGGGTTT GGATCGGGCA CAGGAGCGTT CGCCTTTGGA 
TCATCGAACT CGTCTGTCCA AGGCTTTGGT GGCTGGAGAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021079544.1
CDS	122..6346
Translation	MGDDMDAIVPEREMKDFQFRALKKVRIFDSPEELPKARSSMLAISNKYGMLFAGGAGGLHVFPTKSLLIQNRPGDDPNKIVEPVDSLNVALKFPVHHLALSCDNLTLSACMVSSEYGSVIAFFDVRTFSNQAKPVKLPFTYHKLSKDVGGMVTDMKWNPTVPSMVAVCLADGSIAVLQVTEVVKVCATLPPSAAVTCVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFMIVYAGADVTMETCPDVVMALLPKKEEKHPEIFVNFMEPCYSSCTERQHHYYLNYIEEWDLVLAASAASTEVSILARQSDQTNWESWLLEDSSRAELPVTDKSDDSLPMGVAIDYTNEVEVVINDEKTLPAAPVLLLLSTDGVLCPFYMINQNPGVRSLIKTMELATIEGERQPKSSESFPTTPTSPQAPENLDVPEEASLSLAPVSSAVPTTTFPLPSSGGAPCVFSFGPSSLKPSVSVAGEPPLVPSGSDNPRAALGSGTSTFPFAPPSKGPQALNPAAPSMATPAAPFTFGSSGFKPTLESTPMSSAPNTGMKPSFPPSASSVKVNLNEKFTAVASAPVHSSTSTPSLLPFSSSPKPAASGQLSHPTPLPASSSSIPLKSSVSPSPSGRSTPSVPSSVPSMVQKSPRITPPVVKSGSPQAKSLQPPVTEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKTRTSKACFQVGTLEEMKTLRTESDDLHTFLFEIREATESLHGDISTLKTTLLEGFAGVEEAREQNGRNHDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKKRQRRLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYNQTPPWNLPSTLSTQSSTHSFDSDLESLLKTTVESHTKPSSRVQAKLSPAKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEAISTSSICQPLEGEDARSPCKEEEAVVQVHRHAPVVRTPSIQPSLLPQAMPFAKPHLFHSSSPAVMSSSVSTSASKVIPPGADSTMLATKTVKHGAPGPSHTVAASQAAAAAAMRRQMANQVPAVSTLTESTLKNVPQVVNVQELKSSPAAPSVALGSSVPHSTAKAPHAVLAPVAASQAKQGSLINSFKPSGTTPASCQLSSGDKAAGSGTAKTESTVTSTPSAVGQPNKPFSFAPSGTAFTFGTIASAPSSSLAATPGSVPPTKESNQLEAFSFGGGGKPFCEAIPGSSPPTGALSAAPSTSVTTASPGDSVPPGSRPAASSGITVSTASSKLETPTSKLGEFLFPGSLTGETLGSFSGLRVGQAEDSAKPVSKASSMSLAGSPTPKPPGFSFPGTPVLGKPVEPTVTSSVTPAPAAPVSSLGVSTNSSAAPVFASLPLSSAGPSGLISFGGASLAGSKAGFAFGNQQTSSTVSSAAPTSTSVAPSLPASFPTLSFGGLLSSTSTSSLPVSSGKSPEEATPSAVPDKSGHSEVSASAPSLPVQPQSAQPPQASLQTSDPVKTEPVLVQATDSSPNPPSSAASVVASTEATPVAPGGPDSKTEATAPASTFAGPGQAAVAAAVLPGTGPAASEASGTPSTSSTASSSVPASATETAAFGTATSGSSVCTQPPAASSSSAFSQLSSNTATASSATPMFGQVAASSTATATPQASSSGFGSPAFGASAPAVFGQTAFGQAPAFGQAASNPASGFSFSQPGFSSGPVFGQSVSSTPTSTSANVFGAASSASSPGSFSFGQASTNTGGTLFGQNNPPAFGQSPGFGQGGSVFGSTSATTSTAAPSGFSFCQASGFGSSNTGSVFGQASSTGGSVFGQSSTSSGSVFGSGNSTRGSGFFSGLGGKPSQDAANKNPFSSASGGFGSTTTPNTANLFGNSGAKTFGGFGSSSFGEQKPAGTFSSGGGSVASQGFGFSTPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSSTASFGTLASQNAPTFGSLSQQTSGFGTQNSGFSGFGSGTGAFAFGSSNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_021223885.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus nucleoporin 214 (Nup214), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021223885.1

ORF Insert Sequence:

ATGGGAGACG ATATGGATGC CATAGTCCCT GAGCGGGAGA TGAAGGACTT TCAGTTTAGA 
GCACTGAAGA AAGTAAGGAT CTTTGACTCT CCTGAAGAGT TGCCCAAGGC GCGCTCGAGT 
ATGCTTGCCA TATCCAATAA GTATGGTATG CTCTTTGCTG GTGGCGCCGG TGGCTTGCAT 
GTTTTTCCTA CTAAAAGTCT TCTTATCCAA AACAGACCTG GAGATGATCC CAATAAAATT 
GTTGAGCCAG TCGACAGCTT GAATGTTGCT CTGAAATTCC CAGTACATCA CCTGGCACTG 
AGCTGTGACA ACCTCACACT CTCTGCATGC ATGGTGTCCA GTGAATACGG CTCCGTTATA 
GCTTTTTTTG ATGTTCGCAC ATTTTCGAAT CAGGCTAAAC CAGTGAAACT CCCATTTACC 
TATCACAAGC TTTCAAAGGA TGTAGGTGGT ATGGTGACTG ATATGAAGTG GAACCCCACT 
GTCCCGTCCA TGGTGGCAGT TTGTCTGGCT GATGGCAGTA TTGCTGTCCT GCAAGTTACA 
GAAGTGGTGA AAGTGTGTGC CACTCTTCCT CCCTCGGCAG CAGTTACATG TGTGTGCTGG 
AGTCCCAAAG GAAAGCAGCT GGCAGTAGGG AAGCAGAATG GAACCGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCA TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTAAGAGTCC TGGATGTGCT GTGGATTGGC ACCTATGTCT TCATGATAGT TTATGCTGGT 
GCAGATGTGA CCATGGAAAC CTGTCCAGAT GTGGTGATGG CTTTACTCCC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GCTACAGCAG CTGCACAGAG 
CGACAGCATC ATTACTACCT CAATTACATC GAGGAGTGGG ATTTGGTGCT GGCAGCATCT 
GCAGCGTCAA CAGAAGTTAG CATCCTTGCT CGACAAAGTG ATCAGACTAA TTGGGAATCT 
TGGCTGCTGG AGGACTCTAG TCGAGCTGAA CTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GCGTTGCCAT AGATTATACT AATGAAGTAG AAGTCGTCAT CAACGATGAA 
AAGACCCTTC CTGCTGCTCC GGTTCTCCTG TTACTTTCAA CAGATGGTGT GCTTTGTCCG 
TTTTATATGA TCAATCAAAA TCCTGGGGTT AGGTCCCTAA TCAAGACCAT GGAGCTGGCC 
ACAATAGAAG GAGAGCGACA GCCCAAGTCC TCAGAAAGTT TTCCTACCAC TCCAACCTCC 
CCTCAAGCCC CGGAGAACCT TGATGTCCCA GAAGAAGCCT CTTTGTCCCT TGCACCGGTG 
TCCTCTGCTG TTCCTACCAC CACCTTCCCT TTGCCCTCTT CTGGGGGGGC CCCTTGTGTG 
TTCTCCTTTG GCCCCTCATC CTTAAAGCCA TCTGTGTCTG TGGCTGGGGA GCCTCCTCTG 
GTTCCCTCTG GCTCCGACAA TCCCAGAGCA GCTCTGGGCT CTGGCACCTC CACCTTCCCC 
TTTGCCCCTC CTTCCAAAGG CCCCCAGGCC CTTAACCCTG CAGCACCCTC CATGGCCACA 
CCTGCTGCTC CCTTCACTTT TGGATCATCA GGTTTTAAGC CCACCTTGGA AAGCACACCA 
ATGTCCAGTG CTCCAAACAC AGGGATGAAG CCTTCGTTCC CACCCTCAGC CTCTTCTGTG 
AAAGTCAACC TGAATGAAAA GTTCACCGCT GTGGCCTCTG CGCCTGTCCA TAGCTCCACG 
AGCACACCCT CCCTGTTGCC ATTCTCTTCC TCCCCCAAGC CAGCTGCTTC TGGACAGCTC 
AGCCATCCCA CGCCTCTGCC GGCATCGTCC AGCTCCATTC CTTTGAAGTC TTCAGTCTCT 
CCCTCGCCAT CAGGCCGATC TACTCCGAGT GTTCCAAGCT CAGTTCCCTC CATGGTACAG 
AAATCACCCA GGATAACCCC TCCAGTGGTA AAGTCAGGCT CTCCTCAGGC TAAGTCCCTT 
CAGCCTCCTG TCACAGAGAA ACAGGGACAT CAGTGGAAGG ATTCAGACCC TGTGATGGCT 
GGCATTGGAG AAGAGATTGC ACATTTCCAG AAAGAGCTAG AGGAGTTAAA GACCCGGACC 
TCCAAAGCCT GTTTCCAGGT TGGCACCTTG GAGGAGATGA AGACCCTGCG GACAGAATCT 
GATGACTTGC ACACCTTCCT CTTCGAGATT AGAGAGGCCA CAGAGTCTCT TCATGGAGAC 
ATAAGTACCC TGAAAACAAC TCTGCTGGAG GGCTTTGCTG GTGTTGAAGA AGCCAGAGAA 
CAAAACGGAA GAAACCATGA CTCTGGGTAT CTGCACCTGC TTTATAAAAG ACCATTGGAT 
CCCAAGAGTG AAGCTCAGCT CCAGGAAATC CGTCGTCTTC ATCAGTATGT GAAGTTTGCT 
GTCCAGGATG TAAATGATGT TTTAGACCTG GAGTGGGATC GGCATCTGGA ACAGAAGAAA 
AGACAGAGGC GTTTGCTTGT GCCAGAACGA GAGACACTTT TCAACACACT AGCCAACAAC 
CGGGAGATCA TCAACCAACA GAGGAAGAGG TTGAATCACC TGGTGGACAG TCTGCAGCAG 
CTCCGTCTCT ATAACCAGAC CCCCCCATGG AATCTGCCCT CAACACTCTC AACTCAGAGC 
AGCACTCACA GTTTTGACAG TGACCTTGAA AGCCTGCTAA AAACAACCGT TGAATCTCAC 
ACCAAGCCCT CTTCCAGAGT GCAAGCTAAA CTGTCCCCCG CTAAACAGGC CCAACTGAGG 
AACTTCTTAG CCAAAAGGAA GACCCCACCA GTGAGGTCCA CTGCTCCAGC CAGCCTGTCT 
CGGTCAGCCT TCCTGTCTCA GAGATATTAT GAAGACTTAG ATGAAGCCAT CTCCACATCC 
TCCATCTGTC AGCCCCTGGA GGGAGAAGAT GCCCGGTCTC CCTGCAAAGA GGAGGAAGCT 
GTGGTCCAGG TCCATAGGCA TGCTCCTGTG GTCCGCACGC CTTCTATCCA ACCCAGTCTC 
CTGCCCCAGG CAATGCCTTT TGCTAAGCCT CATCTGTTTC ATAGTTCTTC ACCTGCTGTG 
ATGAGCTCCT CAGTCTCTAC ATCAGCCAGC AAAGTTATTC CTCCAGGAGC CGACAGCACG 
ATGCTGGCAA CCAAAACTGT TAAGCATGGT GCACCAGGTC CTTCCCACAC TGTCGCAGCT 
TCCCAGGCGG CAGCAGCTGC AGCCATGAGA CGGCAGATGG CCAATCAGGT GCCAGCTGTG 
AGCACTCTCA CCGAATCAAC TTTGAAGAAT GTTCCTCAAG TGGTAAACGT ACAGGAACTG 
AAGAGTAGCC CTGCAGCCCC GTCTGTGGCC CTGGGTTCTT CAGTGCCGCA CTCAACAGCC 
AAAGCACCCC ATGCAGTGTT GGCCCCGGTG GCTGCCAGCC AAGCTAAGCA GGGCTCTCTG 
ATAAATTCCT TCAAGCCGTC AGGGACCACA CCAGCATCCT GTCAATTGTC CTCTGGAGAT 
AAAGCTGCAG GTTCAGGCAC AGCCAAGACG GAATCAACCG TGACCTCGAC TCCATCTGCT 
GTGGGGCAGC CCAACAAACC TTTCTCATTT GCTCCCTCAG GGACTGCCTT TACTTTTGGG 
ACGATCGCAT CGGCGCCCTC TTCTAGTCTC GCTGCCACAC CAGGATCAGT ACCCCCCACG 
AAAGAGTCAA ACCAGCTGGA GGCATTTTCA TTTGGCGGGG GAGGCAAACC TTTCTGTGAG 
GCCATTCCTG GAAGCTCTCC TCCCACAGGA GCCTTGTCAG CAGCACCCAG CACTTCTGTA 
ACGACTGCCT CTCCAGGAGA CTCTGTCCCA CCCGGCAGCA GACCTGCAGC TTCCTCTGGA 
ATTACTGTGT CCACTGCCTC TAGCAAATTG GAAACTCCAA CGTCTAAGCT GGGAGAGTTC 
CTGTTCCCAG GCTCCCTGAC TGGAGAAACG CTAGGGAGCT TTTCAGGACT GCGTGTTGGC 
CAAGCTGAGG ACTCTGCAAA GCCAGTCAGT AAGGCCTCAT CCATGAGCCT AGCTGGATCC 
CCGACACCAA AGCCCCCAGG CTTTAGCTTT CCCGGCACTC CAGTGTTAGG GAAGCCTGTG 
GAGCCCACTG TGACATCCTC TGTAACCCCT GCCCCGGCAG CGCCAGTGTC TTCCCTGGGT 
GTGAGCACTA ACAGTTCGGC TGCGCCAGTC TTTGCCAGTC TGCCCCTTAG CAGTGCCGGA 
CCTTCTGGGC TCATCAGTTT TGGTGGGGCA TCTCTGGCCG GTAGCAAAGC TGGTTTCGCA 
TTTGGAAACC AACAGACTAG TAGTACTGTG TCATCAGCTG CCCCAACATC CACCTCAGTT 
GCCCCTTCCC TTCCAGCGTC TTTCCCCACC TTATCATTTG GTGGCCTCCT AAGTTCAACG 
TCTACTTCTT CCCTTCCTGT GTCCTCTGGT AAGAGCCCAG AGGAGGCTAC ACCATCAGCT 
GTGCCTGATA AGTCAGGCCA CAGTGAAGTC TCAGCATCAG CACCCTCCCT TCCAGTACAG 
CCACAATCCG CCCAGCCTCC CCAGGCTTCT CTGCAAACTT CTGACCCTGT CAAAACAGAG 
CCTGTTCTTG TGCAGGCCAC AGACAGCAGC CCCAACCCAC CATCATCTGC AGCCAGTGTT 
GTGGCATCTA CAGAGGCCAC TCCAGTAGCC CCAGGGGGCC CTGACAGCAA GACAGAGGCT 
ACTGCTCCTG CGTCTACCTT TGCGGGGCCT GGGCAGGCTG CTGTCGCAGC GGCTGTTCTC 
CCAGGGACAG GCCCAGCAGC CAGTGAAGCA TCGGGTACTC CTAGCACCTC CAGCACAGCT 
TCCAGCAGCG TCCCAGCTTC TGCCACAGAG ACAGCCGCGT TTGGGACTGC TACTTCTGGC 
TCCTCGGTCT GCACACAGCC ACCTGCTGCC AGTTCTAGCT CAGCGTTCAG CCAACTCTCC 
AGTAACACAG CCACTGCATC CTCTGCCACC CCCATGTTTG GGCAGGTGGC AGCTAGCAGC 
ACAGCCACTG CCACACCACA GGCCAGCAGC TCAGGGTTTG GCAGCCCTGC GTTTGGTGCT 
TCAGCTCCAG CAGTCTTTGG ACAGACAGCC TTTGGGCAGG CCCCGGCCTT TGGGCAAGCA 
GCAAGCAACC CAGCAAGTGG CTTCTCTTTC AGCCAGCCTG GGTTCAGCTC TGGGCCTGTT 
TTTGGCCAGT CTGTTTCCTC TACCCCCACA TCCACCAGTG CGAATGTCTT CGGAGCCGCC 
TCGAGTGCCA GCAGCCCTGG TTCCTTCTCA TTTGGACAGG CTTCTACCAA CACAGGAGGG 
ACACTGTTTG GCCAGAACAA CCCCCCTGCT TTTGGTCAAA GCCCTGGCTT TGGACAGGGA 
GGCTCTGTGT TTGGTAGCAC CTCGGCCACT ACCTCTACAG CAGCACCTTC AGGGTTTAGC 
TTTTGTCAAG CCTCTGGTTT TGGGTCTAGT AATACTGGTT CTGTGTTTGG TCAGGCATCC 
AGTACTGGCG GATCAGTCTT TGGCCAGTCA TCTACTTCCA GTGGTAGTGT GTTTGGGTCT 
GGAAACTCGA CAAGAGGGAG CGGTTTCTTC AGTGGCCTTG GAGGAAAACC CAGCCAGGAT 
GCTGCCAACA AAAACCCATT CAGCTCAGCC AGTGGGGGCT TTGGATCTAC AACCACCCCA 
AATACCGCTA ATCTATTTGG AAACAGTGGC GCTAAGACAT TTGGAGGGTT TGGCAGCTCA 
TCCTTTGGGG AACAGAAGCC TGCTGGCACT TTCAGCTCTG GTGGTGGAAG CGTGGCATCC 
CAAGGCTTTG GATTTTCTAC TCCAAATAAA ACAGGTGGCT TTGGTGCTGC TCCAGTGTTT 
GGCAGCCCTC CTACTTTTGG GGGATCCCCT GGGTTTGGAG GGGTGCCAGC ATTCGGTTCA 
GCCCCGGCCT TTACAAGCCC TCTGGGCTCG ACGGGAGGCA AAGTGTTCGG AGAGGGTACT 
GCGGCTGCCA GCGCAGGAGG ATTCGGGTTT GGGAGCAGCA GCAGCACTGC ATCCTTTGGT 
ACCCTCGCCA GTCAGAACGC GCCAACCTTC GGGTCCTTGT CCCAGCAGAC CTCAGGCTTT 
GGGACCCAGA ACAGTGGCTT CTCTGGGTTT GGATCGGGCA CAGGAGCGTT CGCCTTTGGA 
TCATCGAACT CGTCTGTCCA AGGCTTTGGT GGCTGGAGAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Nup214 cDNA ORF clone, Mesocricetus auratus(golden hamster)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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