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T7 RNA Polymerase

Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for the T7 phage promoter. The enzyme is widely used for the synthesis of specific transcripts from DNA in the 5'→ 3' direction, as well as being a suitable model for studying the mechanisms of transcription. The RNA produced by T7 RNA Polymerase is suitable for many downstream applications. GenScript is offering T7 RNA Polymerase produced by expression in an E. coli strain carrying a plasmid encoding the T7 RNA Polymerase.
$65.00
E00066-5

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Description

Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for the T7 phage promoter. The enzyme is widely used for the synthesis of specific transcripts from DNA in the 5'→ 3' direction, as well as being a suitable model for studying the mechanisms of transcription. The RNA produced by T7 RNA Polymerase is suitable for many downstream applications.

GenScript is offering T7 RNA Polymerase produced by expression in an E. coli strain carrying a plasmid encoding the T7 RNA Polymerase. 
Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Properties
Source Recombinant T7 RNA Polymerase expressed by E.coli
Species phage T7
Molecular Weight ~100 kDa
Unit Definition One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C.
Optimal active temperature 37 °C.
Formulation Supplied as a solution of 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 50% glycerol, pH 8.0 at 25 °C. 
Storage & Stability This product remains stable for up to 12 months at -20 °C. Avoid repeated freeze-thaw cycles.
Quality Control Specifications
AssaySpecifications
AppearanceClear, colorless liquid
Purity≥ 95% as analyzed by SDS-PAGE
Enzyme Activity≥ 50 U/μl
Endotoxin Level≤ 0.1 EU/µg of protein as analyzed by gel clotting method
Residual EndonucleaseNon-detectable
Residual ExonucleaseNon-detectable
Residual RNaseNon-detectable
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Applications
·   Synthesis of the single-strand RNA
·   Synthesis of highly labeled RNA probes
·   Synthesis of precursors of siRNA
·   Synthesis of precursors for RNA splicing reactions
·   Synthesis of capped mRNA when a cap analog is used as a primer
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T7 RNA Polymerase

In vitro transcription of RNA with the recommended reaction conditions, the transcribed RNA can be up to 10 kb. »

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Background
References 1.      J Kochetkov, S. N., E. E. Rusakova, and V. L. Tunitskaya. "Recent studies of T7 RNA polymerase mechanism." FEBS letters 440.3 (1998): 264-267.
2.      Sousa, Rui, Debabrata Patra, and Eileen M. Lafer. "Model for the mechanism of bacteriophage T7 RNAP transcription initiation and termination." Journal of molecular biology 224.2 (1992): 319-334. 

--> 1.      J Kochetkov, S. N., E. E. Rusakova, and V. L. Tunitskaya. "Recent studies of T7 RNA polymerase mechanism." FEBS letters 440.3 (1998): 264-267.
2.      Sousa, Rui, Debabrata Patra, and Eileen M. Lafer. "Model for the mechanism of bacteriophage T7 RNAP transcription initiation and termination." Journal of molecular biology 224.2 (1992): 319-334. 
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