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MonoRab™ Rabbit Anti-Humanized VHH Antibody, MAb

ELISA binding of MonoRab™ Rabbit Anti-Humanized VHH Antibody, mAb with different humanized VHHs.

Coating antigen: Humanized VHHs, 1 µg/ml.
Primary Antibody: MonoRab™ Rabbit Anti-Humanized VHH Antibody, mAb (GenScript, A02165) dilution started from 1 µg/ml.
Secondary Antibody: Mouse Anti-Rabbit IgG Fc Antibody (14H9H10)[HRP], mAb (GenScript,A01856), 1:20,000

MonoRab™ Rabbit Anti-Humanized VHH Antibody, mAb

The product is specific for Humanized VHH antibody and Camelid VHH antibody (Llama, Alpaca and Camel).
A02165
$209.00

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Overview
Specificity The product is specific for Humanized VHH antibodies and Camelid (Llama, Alpaca, and Camel) heavy chain antibodies (IgG2&3), but does not recognize the camelid conventional antibody (IgG1).
Host Species Rabbit
Immunogen VHH antibody
Species Reactivity It has no cross-reactivity with mouse, rat, rabbit, or goat immunoglobulins.
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 1:5,000-1:10,000
Western Blot 1:1,000-1:5,000
Flow Cytometry 1:100-1:1,000

Properties
Form Liquid
Storage Buffer Supplied in PBS (pH 7.2) with 0.02% sodium azide and 50% glycerol.
Concentration 0.5 mg/ml
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Protein A affinity column
Isotype Rabbit IgG
Clonality Monoclonal
Clone ID 45H8
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • MonoRab™ Rabbit Anti-Humanized VHH Antibody, MAb
    • MonoRab™ Rabbit Anti-Humanized VHH Antibody, MAb

    ELISA binding of MonoRab™ Rabbit Anti-Humanized VHH Antibody, mAb with different humanized VHHs.

    Coating antigen: Humanized VHHs, 1 µg/ml.
    Primary Antibody: MonoRab™ Rabbit Anti-Humanized VHH Antibody, mAb (GenScript, A02165) dilution started from 1 µg/ml.
    Secondary Antibody: Mouse Anti-Rabbit IgG Fc Antibody (14H9H10)[HRP], mAb (GenScript,A01856), 1:20,000


Background
Target Background A camelid single-domain antibody (sdAb, called Nanobody by Ablynx, the developer) is a peptide chain of about 110 amino acids long, comprising one variable domain of a heavy-chain antibody, these are called VHH fragments. Like a whole antibody, it is able to bind selectively to a specific antigen. Single-domain antibodies allow a broad range of applications in biotechnical as well as therapeutic uses due to their small size, simple production, and high affinity. However, their sequences may increase the risk of immunogenicity and anti-drug antibody (ADA) development in humans, and thus, sdAbs are routinely humanized during development.
Synonyms Rabbit anti-Humanized VHH, single-domain antibody monoclonal antibody (Min X Ms, Rt, Rb, Gt).
Reference

1. Immunogenicity and humanization of single-domain antibodies.

2. Properties, production, and applications of camelid single-domain antibody fragments. 

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.