MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail (A02014) is specific to Camelid IgG and have no cross-reactivity with mouse, rat, rabbit, goat and human immunoglobulins.

MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail (A02014) is specific to camelid heavy chain antibodies (IgG2&3), but not to the camelid conventional antibody (IgG1).

Lane 1: VHH #1 100 ng
Lane 2: VHH #1 50 ng
Lane 3: VHH #1 25 ng
Lane 4: VHH #2 100 ng
Lane 5: VHH #2 50 ng
Lane 6: VHH #2 25 ng

Primary antibody:
MonoRabᵀᴹ Rabbit Anti-Camelid VHH Cocktail (Genscript, A02014, 0.5μg/ml)
Secondary antibody:
Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb (Genscript, A00098, 1:5,000)

Standard curve of VHH Sandwich ELISA. The VHH Sandwich ELISA assay is developed by using MonoRab™ Rabbit Anti-Camelid VHH Antibody, mAb (GenScript, A01860) and MonoRab™ Rabbit Anti-Camelid VHH Cocktail [HRP] (GenScript, A02016) as the capture and detection antibodies, respectively.

In this ELISA assay, the detection antibody was labeled with horseradish peroxidase (HRP), if you needs an unconjugated antibody you can choose MonoRab™ Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014).
GenScript can provide customized conjugation services for this product per the customer's request.

The sensitivity of detecting VHH is up to 39 pg/ml.

Immunohistochemical analysis of VHH-transduced CAR-T cells infiltrated liver tissue of PDX mouse. VHH was labelled with A02014 at 1 μg/ml, followed by incubation of biotinylated anti-rabbit IgG (H+L) (2.5 μg/ml). The final signal was amplified by ABC (Avidin-Biotin Complex) Kit.

Positive staining was shown in the membrane or cytoplasm of VHH-transduced CAR-T cells infiltrated into liver tissue. Counterstained with hematoxylin.