AmMag™ Quatro ProAb 1300

Highlights

Fully Automated

From incubation to washing, elution, and regeneration

High-Throughput

Up to 12 samples with yields up to 320 mg per protein

Efficient

Purification in 30 minutes

Flexible

Customizable protocols for mini to midi scale

Traditional Resin vs. ProAb Purification Workflow

Product Offerings

AmMag™ Quatro ProAb Ordering Table

Recommended Magnetic Beads for Optimal Purification

Technical Data

• Updated Full-Auto Protein Purify System

  Featured	AmMag™ Quatro 1300 New	AmMag™ SA Discontinued
  Automation	Full automation with integrated mixing and liquid handling	Semi-automation
  Purification time	30 minutes, fully hands-off	45 minutes with manual sample transfers
  Max sample capacity	Up to 65 mL	Up to 50 mL
  Settled MagBeads volume per sample	Up to 6 mL	Up to 2 mL
  Buffer consumption	Optimized, reduced waste	High buffer consumption
  User interface	Simpler and user-friendly	Less intuitive

  Trade-In Your Old Instrument

Instrument performance

• Methodology Consistency Validation

  Consistent Performance between AmMag™ Quatro 1300 Automation and Conventional Manual Magnetic Bead Process.

  

  

  

  Figure 1. Comparison of purification performance between the AmMag™ Quatro ProAb 1300 Automated System and manual magnetic bead purification. Using 30 mL of CHO cell fermentation broth overexpressing Anti-E2 hIgG (antibody titer 923.3 μg/mL by BLI), samples were purified with 2 mL GenScript Protein A Magnetic Beads (Cat# L00695) via both methods. Analysis of antibody recovery rate, protein purity (SEC-HPLC), and binding activity showed consistent results, with both methods achieving >95% purity and comparable activity to positive controls (PC: protein purification via media; GS-1300: AmMag™ Quatro ProAb 1300; GS-Manual: manual purification).

• Intra-run repeatability Validation

  High Repeatability of AmMag™ Quatro 1300 among 12 samples.

  

  

  

  Figure 2. The AmMag™ Quatro ProAb 1300 Automated Purification System demonstrates high consistency across 12 samples processed simultaneously. Using AmMag™ Quatro Elite Protein A Magnetic Beads 2G (Cat# L01074), 12 identical 30 mL CHO cell supernatant samples overexpressing Anti-N protein hIgG were purified in a single run. Protein recovery rate, purity, and activity were measured and showed consistent results across all samples, validating the system’s within-run repeatability. (PC: Positive control purified by protein purification media; GS-1300: AmMag™ Quatro ProAb 1300; GS-Manual: manual purification with GenScript magnetic beads.)

Application

• Sample Compatibility Validation

  Exhibits broad compatibility with protein samples across low-to-high concentration ranges, rendering it suitable for both laboratory research and development (R&D) as well as early-stage process optimization.

  

  

  

  

  

  Figure 3. The AmMag™ Quatro ProAb 1300 Automated Purification System demonstrates broad compatibility with protein samples across a wide concentration range. Using AmMag™ Quatro Elite Protein A Magnetic Beads 2G (Cat# L01074), 30 mL supernatants from three different CHO cell lines overexpressing hIgG with antibody titers ranging from 23 μg/mL to 923 μg/mL were purified. Protein recovery rate, purity, and activity measurements confirmed the system’s effective performance across low-to-high concentration samples, making it suitable for laboratory research, R&D, and early-stage process optimization. (PC: Positive control purified by protein purification media; GS-1300: AmMag™ Quatro ProAb 1300; GS-Manual: manual purification with GenScript magnetic beads.)