NOS1 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS1
Entrez Gene ID	100520934
Full Name	nitric oxide synthase 1
Synonyms	nNOS
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021072698.1	XP_020928357.1	nitric oxide synthase, brain isoform X1
XM_021072696.1	XP_020928355.1	nitric oxide synthase, brain isoform X1
XM_021072699.1	XP_020928358.1	nitric oxide synthase, brain isoform X2
XM_021072697.1	XP_020928356.1	nitric oxide synthase, brain isoform X1
XM_013990334.1	XP_013845788.1	nitric oxide synthase, brain
XM_021072695.1	XP_020928354.1	nitric oxide synthase, brain isoform X1
XM_021072700.1	XP_020928359.1	nitric oxide synthase, brain isoform X2

Pathways from BioSystems
Gene Ontology

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe237687	XM_021072696.1 Latest version!	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSe237687	XM_021072698.1 Latest version!	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSe237687	XM_021072695.1 Latest version!	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSe237687	XM_021072697.1 Latest version!	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSe237688	XM_021072700.1 Latest version!	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSe237688	XM_021072699.1 Latest version!	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OSe42808	XM_013990334.1 Latest version!	Sus scrofa nitric oxide synthase 1 (neuronal) (NOS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS1 ( XM_021072698.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020928357.1 Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSe237687
Clone ID Related Accession (Same CDS sequence)	XM_021072698.1 , XM_021072696.1 , XM_021072697.1 , XM_021072695.1
Accession Version	XM_021072698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TTCCCTCGCC 
CGAGGTTACA CTGAACTGCG CGGTCTGGCT GCAGCTCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTGCGAT TCAATAGTGT TTCCTCATAC TCCGACTCCC GCAAATCATC AGGGGATGGT 
CCAGACCTCA GAGACAACTT TGAGAGCGCC GGGCCCCTGG CAAATGTGAG GTTCTCTGTG 
TTCGGCCTTG GCTCCCGGGC ATACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC 
CTCCTGGAAG AGCTAGGGGG AGAGAGGATC CTAAAGATGA GGGAGGGGGA CGAGCTCTGT 
GGACAGGAGG AGGCTTTCAG GACCTGGGCC AAGAAAGTCT TCAAGGCAGC CTGTGATGTG 
TTCTGCGTGG GAGACGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC 
CGCAGCTGGA AGAGGAACAA GTTCCGCCTC AGCTATGTGG CCGAAGCTCC AGAACTGACC 
CAAGGTCTCT CCAATGTCCA CAAAAAGCGG GTCTCGGCCG CTCGACTCCT CAGCCGTCAA 
AATCTTCAGA GTCCTAAATC CAGCCGATCA ACTATCTTCG TGCGTCTTCA CACCAATGGG 
AATCAGGAGC TTCAGTACCA GCCTGGGGAC CACCTGGGCG TCTTCCCTGG CAACCATGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTCTCAA CCAGCTAGTG 
AAGGTGGAGC TGCTGGAGGA ACGGAACACG GCTTTGGGAG TTATCAGTAA CTGGACAGAC 
GAGCATCGCA TCCCACCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG 
AAGCAGCGCC TGCTCGTCCT TAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATTGTGGA GGTGCTGGAG GAGTTCCCAT CCATCCAGAT GCCCTCTACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTCCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCG 
GACATGTACC CCGACGAGGT GCACCTGACT GTGGCCGTTG TCTCCTACCG CACCCGAGAT 
GGAGAAGGAC CAATTCACCA TGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC 
GAGGTGGTGC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG AAATCCCCAA 
GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG 
CAACGGCAAT TCGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTTTTC 
GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTGCA GGCCAAGAGC 
AAGGGGGTCT TCCGAGAACT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCCGAGGCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATTTACGT CTGTGGGGAC GTCACCATGG CCGCCGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCCGTAG AGGACGCTGG CGTGTTCATC 
AGCAGGCTGA GGGATGACAA CCGGTACCAT GAAGATATTT TCGGAGTCAC CCTGCGCACG 
TATGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATTGAGGA GAGCAAAAAA 
GACACAGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020928357.1
CDS	465..4871
Translation	MENHMFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTAAVDLSHQSAAGKEQTVVVDGATGPGNGPQHIPDHGQEAGSLSHTNGLAPRPPSQDPAKKSAAVTLQGSGENNKLLKEIEPVLNLLSSGSKGINRGEPAKAETKDVEIQVDRDMDGKSHKTLPLGVENDRVFNDLWGKGNMPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLSDTLHLKSTLGTGCTEHICMGSVMLPSQHIRRPEDVHTKEQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIETTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVAFTEICIQQGWKPPRSRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDSSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVHEERKYPEPLRFFPRKGPSLARGYTELRGLAAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLSYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPLNQLVKVELLEERNTALGVISNWTDEHRIPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPSTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQADEVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYKEETLQAKSKGVFRELYTAYSREPDKPKKYVQDILQEQLAEAVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSVEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_021072698.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021072698.1

ORF Insert Sequence:

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TTCCCTCGCC 
CGAGGTTACA CTGAACTGCG CGGTCTGGCT GCAGCTCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTGCGAT TCAATAGTGT TTCCTCATAC TCCGACTCCC GCAAATCATC AGGGGATGGT 
CCAGACCTCA GAGACAACTT TGAGAGCGCC GGGCCCCTGG CAAATGTGAG GTTCTCTGTG 
TTCGGCCTTG GCTCCCGGGC ATACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC 
CTCCTGGAAG AGCTAGGGGG AGAGAGGATC CTAAAGATGA GGGAGGGGGA CGAGCTCTGT 
GGACAGGAGG AGGCTTTCAG GACCTGGGCC AAGAAAGTCT TCAAGGCAGC CTGTGATGTG 
TTCTGCGTGG GAGACGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC 
CGCAGCTGGA AGAGGAACAA GTTCCGCCTC AGCTATGTGG CCGAAGCTCC AGAACTGACC 
CAAGGTCTCT CCAATGTCCA CAAAAAGCGG GTCTCGGCCG CTCGACTCCT CAGCCGTCAA 
AATCTTCAGA GTCCTAAATC CAGCCGATCA ACTATCTTCG TGCGTCTTCA CACCAATGGG 
AATCAGGAGC TTCAGTACCA GCCTGGGGAC CACCTGGGCG TCTTCCCTGG CAACCATGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTCTCAA CCAGCTAGTG 
AAGGTGGAGC TGCTGGAGGA ACGGAACACG GCTTTGGGAG TTATCAGTAA CTGGACAGAC 
GAGCATCGCA TCCCACCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG 
AAGCAGCGCC TGCTCGTCCT TAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATTGTGGA GGTGCTGGAG GAGTTCCCAT CCATCCAGAT GCCCTCTACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTCCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCG 
GACATGTACC CCGACGAGGT GCACCTGACT GTGGCCGTTG TCTCCTACCG CACCCGAGAT 
GGAGAAGGAC CAATTCACCA TGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC 
GAGGTGGTGC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG AAATCCCCAA 
GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG 
CAACGGCAAT TCGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTTTTC 
GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTGCA GGCCAAGAGC 
AAGGGGGTCT TCCGAGAACT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCCGAGGCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATTTACGT CTGTGGGGAC GTCACCATGG CCGCCGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCCGTAG AGGACGCTGG CGTGTTCATC 
AGCAGGCTGA GGGATGACAA CCGGTACCAT GAAGATATTT TCGGAGTCAC CCTGCGCACG 
TATGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATTGAGGA GAGCAAAAAA 
GACACAGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_021072696.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020928355.1 Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe237687
Clone ID Related Accession (Same CDS sequence)	XM_021072698.1 , XM_021072696.1 , XM_021072697.1 , XM_021072695.1
Accession Version	XM_021072696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TTCCCTCGCC 
CGAGGTTACA CTGAACTGCG CGGTCTGGCT GCAGCTCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTGCGAT TCAATAGTGT TTCCTCATAC TCCGACTCCC GCAAATCATC AGGGGATGGT 
CCAGACCTCA GAGACAACTT TGAGAGCGCC GGGCCCCTGG CAAATGTGAG GTTCTCTGTG 
TTCGGCCTTG GCTCCCGGGC ATACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC 
CTCCTGGAAG AGCTAGGGGG AGAGAGGATC CTAAAGATGA GGGAGGGGGA CGAGCTCTGT 
GGACAGGAGG AGGCTTTCAG GACCTGGGCC AAGAAAGTCT TCAAGGCAGC CTGTGATGTG 
TTCTGCGTGG GAGACGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC 
CGCAGCTGGA AGAGGAACAA GTTCCGCCTC AGCTATGTGG CCGAAGCTCC AGAACTGACC 
CAAGGTCTCT CCAATGTCCA CAAAAAGCGG GTCTCGGCCG CTCGACTCCT CAGCCGTCAA 
AATCTTCAGA GTCCTAAATC CAGCCGATCA ACTATCTTCG TGCGTCTTCA CACCAATGGG 
AATCAGGAGC TTCAGTACCA GCCTGGGGAC CACCTGGGCG TCTTCCCTGG CAACCATGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTCTCAA CCAGCTAGTG 
AAGGTGGAGC TGCTGGAGGA ACGGAACACG GCTTTGGGAG TTATCAGTAA CTGGACAGAC 
GAGCATCGCA TCCCACCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG 
AAGCAGCGCC TGCTCGTCCT TAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATTGTGGA GGTGCTGGAG GAGTTCCCAT CCATCCAGAT GCCCTCTACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTCCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCG 
GACATGTACC CCGACGAGGT GCACCTGACT GTGGCCGTTG TCTCCTACCG CACCCGAGAT 
GGAGAAGGAC CAATTCACCA TGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC 
GAGGTGGTGC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG AAATCCCCAA 
GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG 
CAACGGCAAT TCGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTTTTC 
GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTGCA GGCCAAGAGC 
AAGGGGGTCT TCCGAGAACT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCCGAGGCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATTTACGT CTGTGGGGAC GTCACCATGG CCGCCGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCCGTAG AGGACGCTGG CGTGTTCATC 
AGCAGGCTGA GGGATGACAA CCGGTACCAT GAAGATATTT TCGGAGTCAC CCTGCGCACG 
TATGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATTGAGGA GAGCAAAAAA 
GACACAGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020928355.1
CDS	663..5069
Translation	MENHMFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTAAVDLSHQSAAGKEQTVVVDGATGPGNGPQHIPDHGQEAGSLSHTNGLAPRPPSQDPAKKSAAVTLQGSGENNKLLKEIEPVLNLLSSGSKGINRGEPAKAETKDVEIQVDRDMDGKSHKTLPLGVENDRVFNDLWGKGNMPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLSDTLHLKSTLGTGCTEHICMGSVMLPSQHIRRPEDVHTKEQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIETTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVAFTEICIQQGWKPPRSRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDSSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVHEERKYPEPLRFFPRKGPSLARGYTELRGLAAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLSYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPLNQLVKVELLEERNTALGVISNWTDEHRIPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPSTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQADEVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYKEETLQAKSKGVFRELYTAYSREPDKPKKYVQDILQEQLAEAVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSVEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_021072696.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021072696.1

ORF Insert Sequence:

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TTCCCTCGCC 
CGAGGTTACA CTGAACTGCG CGGTCTGGCT GCAGCTCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTGCGAT TCAATAGTGT TTCCTCATAC TCCGACTCCC GCAAATCATC AGGGGATGGT 
CCAGACCTCA GAGACAACTT TGAGAGCGCC GGGCCCCTGG CAAATGTGAG GTTCTCTGTG 
TTCGGCCTTG GCTCCCGGGC ATACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC 
CTCCTGGAAG AGCTAGGGGG AGAGAGGATC CTAAAGATGA GGGAGGGGGA CGAGCTCTGT 
GGACAGGAGG AGGCTTTCAG GACCTGGGCC AAGAAAGTCT TCAAGGCAGC CTGTGATGTG 
TTCTGCGTGG GAGACGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC 
CGCAGCTGGA AGAGGAACAA GTTCCGCCTC AGCTATGTGG CCGAAGCTCC AGAACTGACC 
CAAGGTCTCT CCAATGTCCA CAAAAAGCGG GTCTCGGCCG CTCGACTCCT CAGCCGTCAA 
AATCTTCAGA GTCCTAAATC CAGCCGATCA ACTATCTTCG TGCGTCTTCA CACCAATGGG 
AATCAGGAGC TTCAGTACCA GCCTGGGGAC CACCTGGGCG TCTTCCCTGG CAACCATGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTCTCAA CCAGCTAGTG 
AAGGTGGAGC TGCTGGAGGA ACGGAACACG GCTTTGGGAG TTATCAGTAA CTGGACAGAC 
GAGCATCGCA TCCCACCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG 
AAGCAGCGCC TGCTCGTCCT TAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATTGTGGA GGTGCTGGAG GAGTTCCCAT CCATCCAGAT GCCCTCTACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTCCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCG 
GACATGTACC CCGACGAGGT GCACCTGACT GTGGCCGTTG TCTCCTACCG CACCCGAGAT 
GGAGAAGGAC CAATTCACCA TGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC 
GAGGTGGTGC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG AAATCCCCAA 
GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG 
CAACGGCAAT TCGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTTTTC 
GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTGCA GGCCAAGAGC 
AAGGGGGTCT TCCGAGAACT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCCGAGGCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATTTACGT CTGTGGGGAC GTCACCATGG CCGCCGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCCGTAG AGGACGCTGG CGTGTTCATC 
AGCAGGCTGA GGGATGACAA CCGGTACCAT GAAGATATTT TCGGAGTCAC CCTGCGCACG 
TATGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATTGAGGA GAGCAAAAAA 
GACACAGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_021072697.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020928356.1 Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

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CloneID	OSe237687
Clone ID Related Accession (Same CDS sequence)	XM_021072698.1 , XM_021072696.1 , XM_021072697.1 , XM_021072695.1
Accession Version	XM_021072697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TTCCCTCGCC 
CGAGGTTACA CTGAACTGCG CGGTCTGGCT GCAGCTCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTGCGAT TCAATAGTGT TTCCTCATAC TCCGACTCCC GCAAATCATC AGGGGATGGT 
CCAGACCTCA GAGACAACTT TGAGAGCGCC GGGCCCCTGG CAAATGTGAG GTTCTCTGTG 
TTCGGCCTTG GCTCCCGGGC ATACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC 
CTCCTGGAAG AGCTAGGGGG AGAGAGGATC CTAAAGATGA GGGAGGGGGA CGAGCTCTGT 
GGACAGGAGG AGGCTTTCAG GACCTGGGCC AAGAAAGTCT TCAAGGCAGC CTGTGATGTG 
TTCTGCGTGG GAGACGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC 
CGCAGCTGGA AGAGGAACAA GTTCCGCCTC AGCTATGTGG CCGAAGCTCC AGAACTGACC 
CAAGGTCTCT CCAATGTCCA CAAAAAGCGG GTCTCGGCCG CTCGACTCCT CAGCCGTCAA 
AATCTTCAGA GTCCTAAATC CAGCCGATCA ACTATCTTCG TGCGTCTTCA CACCAATGGG 
AATCAGGAGC TTCAGTACCA GCCTGGGGAC CACCTGGGCG TCTTCCCTGG CAACCATGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTCTCAA CCAGCTAGTG 
AAGGTGGAGC TGCTGGAGGA ACGGAACACG GCTTTGGGAG TTATCAGTAA CTGGACAGAC 
GAGCATCGCA TCCCACCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG 
AAGCAGCGCC TGCTCGTCCT TAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATTGTGGA GGTGCTGGAG GAGTTCCCAT CCATCCAGAT GCCCTCTACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTCCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCG 
GACATGTACC CCGACGAGGT GCACCTGACT GTGGCCGTTG TCTCCTACCG CACCCGAGAT 
GGAGAAGGAC CAATTCACCA TGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC 
GAGGTGGTGC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG AAATCCCCAA 
GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG 
CAACGGCAAT TCGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTTTTC 
GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTGCA GGCCAAGAGC 
AAGGGGGTCT TCCGAGAACT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCCGAGGCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATTTACGT CTGTGGGGAC GTCACCATGG CCGCCGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCCGTAG AGGACGCTGG CGTGTTCATC 
AGCAGGCTGA GGGATGACAA CCGGTACCAT GAAGATATTT TCGGAGTCAC CCTGCGCACG 
TATGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATTGAGGA GAGCAAAAAA 
GACACAGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020928356.1
CDS	333..4739
Translation	MENHMFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTAAVDLSHQSAAGKEQTVVVDGATGPGNGPQHIPDHGQEAGSLSHTNGLAPRPPSQDPAKKSAAVTLQGSGENNKLLKEIEPVLNLLSSGSKGINRGEPAKAETKDVEIQVDRDMDGKSHKTLPLGVENDRVFNDLWGKGNMPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLSDTLHLKSTLGTGCTEHICMGSVMLPSQHIRRPEDVHTKEQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIETTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVAFTEICIQQGWKPPRSRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDSSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVHEERKYPEPLRFFPRKGPSLARGYTELRGLAAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLSYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPLNQLVKVELLEERNTALGVISNWTDEHRIPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPSTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQADEVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYKEETLQAKSKGVFRELYTAYSREPDKPKKYVQDILQEQLAEAVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSVEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_021072697.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021072697.1

ORF Insert Sequence:

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TTCCCTCGCC 
CGAGGTTACA CTGAACTGCG CGGTCTGGCT GCAGCTCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTGCGAT TCAATAGTGT TTCCTCATAC TCCGACTCCC GCAAATCATC AGGGGATGGT 
CCAGACCTCA GAGACAACTT TGAGAGCGCC GGGCCCCTGG CAAATGTGAG GTTCTCTGTG 
TTCGGCCTTG GCTCCCGGGC ATACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC 
CTCCTGGAAG AGCTAGGGGG AGAGAGGATC CTAAAGATGA GGGAGGGGGA CGAGCTCTGT 
GGACAGGAGG AGGCTTTCAG GACCTGGGCC AAGAAAGTCT TCAAGGCAGC CTGTGATGTG 
TTCTGCGTGG GAGACGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC 
CGCAGCTGGA AGAGGAACAA GTTCCGCCTC AGCTATGTGG CCGAAGCTCC AGAACTGACC 
CAAGGTCTCT CCAATGTCCA CAAAAAGCGG GTCTCGGCCG CTCGACTCCT CAGCCGTCAA 
AATCTTCAGA GTCCTAAATC CAGCCGATCA ACTATCTTCG TGCGTCTTCA CACCAATGGG 
AATCAGGAGC TTCAGTACCA GCCTGGGGAC CACCTGGGCG TCTTCCCTGG CAACCATGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTCTCAA CCAGCTAGTG 
AAGGTGGAGC TGCTGGAGGA ACGGAACACG GCTTTGGGAG TTATCAGTAA CTGGACAGAC 
GAGCATCGCA TCCCACCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG 
AAGCAGCGCC TGCTCGTCCT TAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATTGTGGA GGTGCTGGAG GAGTTCCCAT CCATCCAGAT GCCCTCTACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTCCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCG 
GACATGTACC CCGACGAGGT GCACCTGACT GTGGCCGTTG TCTCCTACCG CACCCGAGAT 
GGAGAAGGAC CAATTCACCA TGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC 
GAGGTGGTGC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG AAATCCCCAA 
GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG 
CAACGGCAAT TCGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTTTTC 
GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTGCA GGCCAAGAGC 
AAGGGGGTCT TCCGAGAACT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCCGAGGCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATTTACGT CTGTGGGGAC GTCACCATGG CCGCCGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCCGTAG AGGACGCTGG CGTGTTCATC 
AGCAGGCTGA GGGATGACAA CCGGTACCAT GAAGATATTT TCGGAGTCAC CCTGCGCACG 
TATGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATTGAGGA GAGCAAAAAA 
GACACAGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_021072695.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020928354.1 Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

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CloneID	OSe237687
Clone ID Related Accession (Same CDS sequence)	XM_021072698.1 , XM_021072696.1 , XM_021072697.1 , XM_021072695.1
Accession Version	XM_021072695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TTCCCTCGCC 
CGAGGTTACA CTGAACTGCG CGGTCTGGCT GCAGCTCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTGCGAT TCAATAGTGT TTCCTCATAC TCCGACTCCC GCAAATCATC AGGGGATGGT 
CCAGACCTCA GAGACAACTT TGAGAGCGCC GGGCCCCTGG CAAATGTGAG GTTCTCTGTG 
TTCGGCCTTG GCTCCCGGGC ATACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC 
CTCCTGGAAG AGCTAGGGGG AGAGAGGATC CTAAAGATGA GGGAGGGGGA CGAGCTCTGT 
GGACAGGAGG AGGCTTTCAG GACCTGGGCC AAGAAAGTCT TCAAGGCAGC CTGTGATGTG 
TTCTGCGTGG GAGACGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC 
CGCAGCTGGA AGAGGAACAA GTTCCGCCTC AGCTATGTGG CCGAAGCTCC AGAACTGACC 
CAAGGTCTCT CCAATGTCCA CAAAAAGCGG GTCTCGGCCG CTCGACTCCT CAGCCGTCAA 
AATCTTCAGA GTCCTAAATC CAGCCGATCA ACTATCTTCG TGCGTCTTCA CACCAATGGG 
AATCAGGAGC TTCAGTACCA GCCTGGGGAC CACCTGGGCG TCTTCCCTGG CAACCATGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTCTCAA CCAGCTAGTG 
AAGGTGGAGC TGCTGGAGGA ACGGAACACG GCTTTGGGAG TTATCAGTAA CTGGACAGAC 
GAGCATCGCA TCCCACCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG 
AAGCAGCGCC TGCTCGTCCT TAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATTGTGGA GGTGCTGGAG GAGTTCCCAT CCATCCAGAT GCCCTCTACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTCCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCG 
GACATGTACC CCGACGAGGT GCACCTGACT GTGGCCGTTG TCTCCTACCG CACCCGAGAT 
GGAGAAGGAC CAATTCACCA TGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC 
GAGGTGGTGC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG AAATCCCCAA 
GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG 
CAACGGCAAT TCGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTTTTC 
GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTGCA GGCCAAGAGC 
AAGGGGGTCT TCCGAGAACT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCCGAGGCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATTTACGT CTGTGGGGAC GTCACCATGG CCGCCGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCCGTAG AGGACGCTGG CGTGTTCATC 
AGCAGGCTGA GGGATGACAA CCGGTACCAT GAAGATATTT TCGGAGTCAC CCTGCGCACG 
TATGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATTGAGGA GAGCAAAAAA 
GACACAGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020928354.1
CDS	817..5223
Translation	MENHMFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTAAVDLSHQSAAGKEQTVVVDGATGPGNGPQHIPDHGQEAGSLSHTNGLAPRPPSQDPAKKSAAVTLQGSGENNKLLKEIEPVLNLLSSGSKGINRGEPAKAETKDVEIQVDRDMDGKSHKTLPLGVENDRVFNDLWGKGNMPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLSDTLHLKSTLGTGCTEHICMGSVMLPSQHIRRPEDVHTKEQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIETTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVAFTEICIQQGWKPPRSRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDSSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVHEERKYPEPLRFFPRKGPSLARGYTELRGLAAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLSYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPLNQLVKVELLEERNTALGVISNWTDEHRIPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPSTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQADEVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYKEETLQAKSKGVFRELYTAYSREPDKPKKYVQDILQEQLAEAVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSVEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_021072695.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021072695.1

ORF Insert Sequence:

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TTCCCTCGCC 
CGAGGTTACA CTGAACTGCG CGGTCTGGCT GCAGCTCGTG ACAGCCAGCA CAGGAGCTAC 
AAGGTGCGAT TCAATAGTGT TTCCTCATAC TCCGACTCCC GCAAATCATC AGGGGATGGT 
CCAGACCTCA GAGACAACTT TGAGAGCGCC GGGCCCCTGG CAAATGTGAG GTTCTCTGTG 
TTCGGCCTTG GCTCCCGGGC ATACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC 
CTCCTGGAAG AGCTAGGGGG AGAGAGGATC CTAAAGATGA GGGAGGGGGA CGAGCTCTGT 
GGACAGGAGG AGGCTTTCAG GACCTGGGCC AAGAAAGTCT TCAAGGCAGC CTGTGATGTG 
TTCTGCGTGG GAGACGACGT CAACATTGAG AAGGCGAACA ACTCCCTCAT CAGCAATGAC 
CGCAGCTGGA AGAGGAACAA GTTCCGCCTC AGCTATGTGG CCGAAGCTCC AGAACTGACC 
CAAGGTCTCT CCAATGTCCA CAAAAAGCGG GTCTCGGCCG CTCGACTCCT CAGCCGTCAA 
AATCTTCAGA GTCCTAAATC CAGCCGATCA ACTATCTTCG TGCGTCTTCA CACCAATGGG 
AATCAGGAGC TTCAGTACCA GCCTGGGGAC CACCTGGGCG TCTTCCCTGG CAACCATGAG 
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTCTCAA CCAGCTAGTG 
AAGGTGGAGC TGCTGGAGGA ACGGAACACG GCTTTGGGAG TTATCAGTAA CTGGACAGAC 
GAGCATCGCA TCCCACCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC 
ACGCCTCCAA CGCCACTGCA GCTGCAGCAG TTTGCCTCCC TGGCCACCAG TGAGAAAGAG 
AAGCAGCGCC TGCTCGTCCT TAGCAAGGGT CTGCAGGAGT ACGAGGAATG GAAATGGGGC 
AAGAACCCCA CCATTGTGGA GGTGCTGGAG GAGTTCCCAT CCATCCAGAT GCCCTCTACC 
CTGCTCCTGA CCCAGCTGTC CCTGCTCCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCG 
GACATGTACC CCGACGAGGT GCACCTGACT GTGGCCGTTG TCTCCTACCG CACCCGAGAT 
GGAGAAGGAC CAATTCACCA TGGCGTGTGC TCCTCCTGGC TCAACCGGAT CCAGGCTGAC 
GAGGTGGTGC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG AAATCCCCAA 
GTGCCCTGTA TCCTGGTTGG CCCAGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG 
CAACGGCAAT TCGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTTTTC 
GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTGCA GGCCAAGAGC 
AAGGGGGTCT TCCGAGAACT GTACACAGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG 
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCCGAGGCTG TGTACCGAGC CCTGAAGGAG 
CAAGGGGGCC ACATTTACGT CTGTGGGGAC GTCACCATGG CCGCCGATGT CCTCAAAGCC 
ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCCGTAG AGGACGCTGG CGTGTTCATC 
AGCAGGCTGA GGGATGACAA CCGGTACCAT GAAGATATTT TCGGAGTCAC CCTGCGCACG 
TATGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATTGAGGA GAGCAAAAAA 
GACACAGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_021072699.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020928358.1 Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSe237688
Clone ID Related Accession (Same CDS sequence)	XM_021072699.1 , XM_021072700.1
Accession Version	XM_021072699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	nitric oxide synthase, brain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGAGCTACAA GGTGCGATTC AATAGTGTTT CCTCATACTC CGACTCCCGC 
AAATCATCAG GGGATGGTCC AGACCTCAGA GACAACTTTG AGAGCGCCGG GCCCCTGGCA 
AATGTGAGGT TCTCTGTGTT CGGCCTTGGC TCCCGGGCAT ACCCTCACTT CTGCGCCTTT 
GGACACGCCG TGGACACCCT CCTGGAAGAG CTAGGGGGAG AGAGGATCCT AAAGATGAGG 
GAGGGGGACG AGCTCTGTGG ACAGGAGGAG GCTTTCAGGA CCTGGGCCAA GAAAGTCTTC 
AAGGCAGCCT GTGATGTGTT CTGCGTGGGA GACGACGTCA ACATTGAGAA GGCGAACAAC 
TCCCTCATCA GCAATGACCG CAGCTGGAAG AGGAACAAGT TCCGCCTCAG CTATGTGGCC 
GAAGCTCCAG AACTGACCCA AGGTCTCTCC AATGTCCACA AAAAGCGGGT CTCGGCCGCT 
CGACTCCTCA GCCGTCAAAA TCTTCAGAGT CCTAAATCCA GCCGATCAAC TATCTTCGTG 
CGTCTTCACA CCAATGGGAA TCAGGAGCTT CAGTACCAGC CTGGGGACCA CCTGGGCGTC 
TTCCCTGGCA ACCATGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCACCC 
CCTCTCAACC AGCTAGTGAA GGTGGAGCTG CTGGAGGAAC GGAACACGGC TTTGGGAGTT 
ATCAGTAACT GGACAGACGA GCATCGCATC CCACCCTGCA CCATCTTCCA GGCCTTCAAG 
TACTACCTGG ACATCACCAC GCCTCCAACG CCACTGCAGC TGCAGCAGTT TGCCTCCCTG 
GCCACCAGTG AGAAAGAGAA GCAGCGCCTG CTCGTCCTTA GCAAGGGTCT GCAGGAGTAC 
GAGGAATGGA AATGGGGCAA GAACCCCACC ATTGTGGAGG TGCTGGAGGA GTTCCCATCC 
ATCCAGATGC CCTCTACCCT GCTCCTGACC CAGCTGTCCC TGCTCCAGCC CCGCTACTAT 
TCCATCAGCT CCTCCCCGGA CATGTACCCC GACGAGGTGC ACCTGACTGT GGCCGTTGTC 
TCCTACCGCA CCCGAGATGG AGAAGGACCA ATTCACCATG GCGTGTGCTC CTCCTGGCTC 
AACCGGATCC AGGCTGACGA GGTGGTGCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC 
CTGCCCCGAA ATCCCCAAGT GCCCTGTATC CTGGTTGGCC CAGGCACTGG CATTGCCCCT 
TTCCGAAGCT TCTGGCAGCA ACGGCAATTC GATATCCAAC ACAAAGGAAT GAGTCCCTGC 
CCCATGGTCC TGGTTTTCGG GTGCCGGCAG TCCAAGATAG ACCACATCTA CAAGGAGGAG 
ACCCTGCAGG CCAAGAGCAA GGGGGTCTTC CGAGAACTGT ACACAGCCTA CTCCCGGGAG 
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC CGAGGCTGTG 
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATTTACGTCT GTGGGGACGT CACCATGGCC 
GCCGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAACT CTCCGTAGAG 
GACGCTGGCG TGTTCATCAG CAGGCTGAGG GATGACAACC GGTACCATGA AGATATTTTC 
GGAGTCACCC TGCGCACGTA TGAAGTGACC AACCGCCTTA GATCTGAATC CATTGCCTTC 
ATTGAGGAGA GCAAAAAAGA CACAGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020928358.1
CDS	473..4777
Translation	MENHMFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTAAVDLSHQSAAGKEQTVVVDGATGPGNGPQHIPDHGQEAGSLSHTNGLAPRPPSQDPAKKSAAVTLQGSGENNKLLKEIEPVLNLLSSGSKGINRGEPAKAETKDVEIQVDRDMDGKSHKTLPLGVENDRVFNDLWGKGNMPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLSDTLHLKSTLGTGCTEHICMGSVMLPSQHIRRPEDVHTKEQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIETTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVAFTEICIQQGWKPPRSRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDSSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVHEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLSYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPLNQLVKVELLEERNTALGVISNWTDEHRIPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPSTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQADEVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYKEETLQAKSKGVFRELYTAYSREPDKPKKYVQDILQEQLAEAVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSVEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_021072699.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021072699.1

ORF Insert Sequence:

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGAGCTACAA GGTGCGATTC AATAGTGTTT CCTCATACTC CGACTCCCGC 
AAATCATCAG GGGATGGTCC AGACCTCAGA GACAACTTTG AGAGCGCCGG GCCCCTGGCA 
AATGTGAGGT TCTCTGTGTT CGGCCTTGGC TCCCGGGCAT ACCCTCACTT CTGCGCCTTT 
GGACACGCCG TGGACACCCT CCTGGAAGAG CTAGGGGGAG AGAGGATCCT AAAGATGAGG 
GAGGGGGACG AGCTCTGTGG ACAGGAGGAG GCTTTCAGGA CCTGGGCCAA GAAAGTCTTC 
AAGGCAGCCT GTGATGTGTT CTGCGTGGGA GACGACGTCA ACATTGAGAA GGCGAACAAC 
TCCCTCATCA GCAATGACCG CAGCTGGAAG AGGAACAAGT TCCGCCTCAG CTATGTGGCC 
GAAGCTCCAG AACTGACCCA AGGTCTCTCC AATGTCCACA AAAAGCGGGT CTCGGCCGCT 
CGACTCCTCA GCCGTCAAAA TCTTCAGAGT CCTAAATCCA GCCGATCAAC TATCTTCGTG 
CGTCTTCACA CCAATGGGAA TCAGGAGCTT CAGTACCAGC CTGGGGACCA CCTGGGCGTC 
TTCCCTGGCA ACCATGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCACCC 
CCTCTCAACC AGCTAGTGAA GGTGGAGCTG CTGGAGGAAC GGAACACGGC TTTGGGAGTT 
ATCAGTAACT GGACAGACGA GCATCGCATC CCACCCTGCA CCATCTTCCA GGCCTTCAAG 
TACTACCTGG ACATCACCAC GCCTCCAACG CCACTGCAGC TGCAGCAGTT TGCCTCCCTG 
GCCACCAGTG AGAAAGAGAA GCAGCGCCTG CTCGTCCTTA GCAAGGGTCT GCAGGAGTAC 
GAGGAATGGA AATGGGGCAA GAACCCCACC ATTGTGGAGG TGCTGGAGGA GTTCCCATCC 
ATCCAGATGC CCTCTACCCT GCTCCTGACC CAGCTGTCCC TGCTCCAGCC CCGCTACTAT 
TCCATCAGCT CCTCCCCGGA CATGTACCCC GACGAGGTGC ACCTGACTGT GGCCGTTGTC 
TCCTACCGCA CCCGAGATGG AGAAGGACCA ATTCACCATG GCGTGTGCTC CTCCTGGCTC 
AACCGGATCC AGGCTGACGA GGTGGTGCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC 
CTGCCCCGAA ATCCCCAAGT GCCCTGTATC CTGGTTGGCC CAGGCACTGG CATTGCCCCT 
TTCCGAAGCT TCTGGCAGCA ACGGCAATTC GATATCCAAC ACAAAGGAAT GAGTCCCTGC 
CCCATGGTCC TGGTTTTCGG GTGCCGGCAG TCCAAGATAG ACCACATCTA CAAGGAGGAG 
ACCCTGCAGG CCAAGAGCAA GGGGGTCTTC CGAGAACTGT ACACAGCCTA CTCCCGGGAG 
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC CGAGGCTGTG 
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATTTACGTCT GTGGGGACGT CACCATGGCC 
GCCGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAACT CTCCGTAGAG 
GACGCTGGCG TGTTCATCAG CAGGCTGAGG GATGACAACC GGTACCATGA AGATATTTTC 
GGAGTCACCC TGCGCACGTA TGAAGTGACC AACCGCCTTA GATCTGAATC CATTGCCTTC 
ATTGAGGAGA GCAAAAAAGA CACAGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_021072700.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020928359.1 Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OSe237688
Clone ID Related Accession (Same CDS sequence)	XM_021072699.1 , XM_021072700.1
Accession Version	XM_021072700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	nitric oxide synthase, brain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGAGCTACAA GGTGCGATTC AATAGTGTTT CCTCATACTC CGACTCCCGC 
AAATCATCAG GGGATGGTCC AGACCTCAGA GACAACTTTG AGAGCGCCGG GCCCCTGGCA 
AATGTGAGGT TCTCTGTGTT CGGCCTTGGC TCCCGGGCAT ACCCTCACTT CTGCGCCTTT 
GGACACGCCG TGGACACCCT CCTGGAAGAG CTAGGGGGAG AGAGGATCCT AAAGATGAGG 
GAGGGGGACG AGCTCTGTGG ACAGGAGGAG GCTTTCAGGA CCTGGGCCAA GAAAGTCTTC 
AAGGCAGCCT GTGATGTGTT CTGCGTGGGA GACGACGTCA ACATTGAGAA GGCGAACAAC 
TCCCTCATCA GCAATGACCG CAGCTGGAAG AGGAACAAGT TCCGCCTCAG CTATGTGGCC 
GAAGCTCCAG AACTGACCCA AGGTCTCTCC AATGTCCACA AAAAGCGGGT CTCGGCCGCT 
CGACTCCTCA GCCGTCAAAA TCTTCAGAGT CCTAAATCCA GCCGATCAAC TATCTTCGTG 
CGTCTTCACA CCAATGGGAA TCAGGAGCTT CAGTACCAGC CTGGGGACCA CCTGGGCGTC 
TTCCCTGGCA ACCATGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCACCC 
CCTCTCAACC AGCTAGTGAA GGTGGAGCTG CTGGAGGAAC GGAACACGGC TTTGGGAGTT 
ATCAGTAACT GGACAGACGA GCATCGCATC CCACCCTGCA CCATCTTCCA GGCCTTCAAG 
TACTACCTGG ACATCACCAC GCCTCCAACG CCACTGCAGC TGCAGCAGTT TGCCTCCCTG 
GCCACCAGTG AGAAAGAGAA GCAGCGCCTG CTCGTCCTTA GCAAGGGTCT GCAGGAGTAC 
GAGGAATGGA AATGGGGCAA GAACCCCACC ATTGTGGAGG TGCTGGAGGA GTTCCCATCC 
ATCCAGATGC CCTCTACCCT GCTCCTGACC CAGCTGTCCC TGCTCCAGCC CCGCTACTAT 
TCCATCAGCT CCTCCCCGGA CATGTACCCC GACGAGGTGC ACCTGACTGT GGCCGTTGTC 
TCCTACCGCA CCCGAGATGG AGAAGGACCA ATTCACCATG GCGTGTGCTC CTCCTGGCTC 
AACCGGATCC AGGCTGACGA GGTGGTGCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC 
CTGCCCCGAA ATCCCCAAGT GCCCTGTATC CTGGTTGGCC CAGGCACTGG CATTGCCCCT 
TTCCGAAGCT TCTGGCAGCA ACGGCAATTC GATATCCAAC ACAAAGGAAT GAGTCCCTGC 
CCCATGGTCC TGGTTTTCGG GTGCCGGCAG TCCAAGATAG ACCACATCTA CAAGGAGGAG 
ACCCTGCAGG CCAAGAGCAA GGGGGTCTTC CGAGAACTGT ACACAGCCTA CTCCCGGGAG 
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC CGAGGCTGTG 
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATTTACGTCT GTGGGGACGT CACCATGGCC 
GCCGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAACT CTCCGTAGAG 
GACGCTGGCG TGTTCATCAG CAGGCTGAGG GATGACAACC GGTACCATGA AGATATTTTC 
GGAGTCACCC TGCGCACGTA TGAAGTGACC AACCGCCTTA GATCTGAATC CATTGCCTTC 
ATTGAGGAGA GCAAAAAAGA CACAGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020928359.1
CDS	359..4663
Translation	MENHMFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTAAVDLSHQSAAGKEQTVVVDGATGPGNGPQHIPDHGQEAGSLSHTNGLAPRPPSQDPAKKSAAVTLQGSGENNKLLKEIEPVLNLLSSGSKGINRGEPAKAETKDVEIQVDRDMDGKSHKTLPLGVENDRVFNDLWGKGNMPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLSDTLHLKSTLGTGCTEHICMGSVMLPSQHIRRPEDVHTKEQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIETTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVAFTEICIQQGWKPPRSRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDSSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVHEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLSYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPLNQLVKVELLEERNTALGVISNWTDEHRIPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPSTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQADEVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYKEETLQAKSKGVFRELYTAYSREPDKPKKYVQDILQEQLAEAVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSVEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_021072700.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa nitric oxide synthase 1 (NOS1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021072700.1

ORF Insert Sequence:

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGATT CGGCTCCAAA 
GCCCACATGG AGAGGCTGGA GGAGGTGAAC AAAGAGATTG AAACCACCAG CACCTACCAG 
CTCAAGGACA CAGAGCTCAT CTACGGGGCC AAGCACGCCT GGCGGAATGC CTCCCGCTGC 
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG TACCACGGCT 
CACGGGATGT TCAACTACAT CTGTAACCAC ATCAAGTATG CCACCAACAA AGGGAACCTC 
AGATCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCATGACTT CCGAGTCTGG 
AACTCCCAGC TCATCCGCTA CGCCGGCTAC AAGCAGCCCG ATGGCTCCAT CCTGGGGGAT 
CCAGCCAATG TGGCATTCAC GGAGATCTGT ATCCAGCAGG GCTGGAAGCC CCCAAGGAGC 
CGCTTCGATG TCCTGCCACT CCTGCTCCAG GCCAATGGCA ACGACCCTGA GCTCTTCCAG 
ATCCCTCCAG AGCTGGTGTT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG 
GACCTTGGGC TGAAGTGGTA CGGCCTCCCT GCTGTGTCCA ACATGCTACT GGAGATCGGC 
GGCCTGGAGT TCAGTGCCTG CCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC 
CGTGACTACT GTGACAGCTC TCGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC 
TTGGACATGA GGAAGACATC CTCCCTGTGG AAGGACCAAG CACTGGTGGA GATCAACATT 
GCGGTTCTGT ACAGCTTCCA GAGTGACAAA GTGACCATCG TGGACCACCA CTCTGCCACC 
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGCGGCTG CCCTGCCGAC 
TGGGTGTGGA TCGTGCCCCC CATGTCCGGC AGCATCACCC CTGTCTTCCA CCAGGAGATG 
CTCAACTACC GGCTTACCCC CTCCTTTGAA TACCAGCCTG ACCCCTGGAA CACCCACGTC 
TGGAAAGGCA CCAACGGGAC CCCCACAAAA CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA 
GAAGCCGTCA AGTTCTCAGC CAAGCTGATG GGACAGGCTA TGGCCAAGAG GGTCAAGGCT 
ACCATCCTTT ATGCCACAGA GACGGGCAAA TCACAGGCTT ATGCTAAAAC CTTGTGTGAG 
ATCTTCAAAC ATGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATCGTACAC 
CTGGAACATG AAACTCTGGT CCTAGTGGTT ACCAGCACCT TTGGCAATGG AGACCCCCCT 
GAGAATGGGG AGAAGTTTGG CTGCGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAC 
GAGGAAAGGA AGAGCTACAA GGTGCGATTC AATAGTGTTT CCTCATACTC CGACTCCCGC 
AAATCATCAG GGGATGGTCC AGACCTCAGA GACAACTTTG AGAGCGCCGG GCCCCTGGCA 
AATGTGAGGT TCTCTGTGTT CGGCCTTGGC TCCCGGGCAT ACCCTCACTT CTGCGCCTTT 
GGACACGCCG TGGACACCCT CCTGGAAGAG CTAGGGGGAG AGAGGATCCT AAAGATGAGG 
GAGGGGGACG AGCTCTGTGG ACAGGAGGAG GCTTTCAGGA CCTGGGCCAA GAAAGTCTTC 
AAGGCAGCCT GTGATGTGTT CTGCGTGGGA GACGACGTCA ACATTGAGAA GGCGAACAAC 
TCCCTCATCA GCAATGACCG CAGCTGGAAG AGGAACAAGT TCCGCCTCAG CTATGTGGCC 
GAAGCTCCAG AACTGACCCA AGGTCTCTCC AATGTCCACA AAAAGCGGGT CTCGGCCGCT 
CGACTCCTCA GCCGTCAAAA TCTTCAGAGT CCTAAATCCA GCCGATCAAC TATCTTCGTG 
CGTCTTCACA CCAATGGGAA TCAGGAGCTT CAGTACCAGC CTGGGGACCA CCTGGGCGTC 
TTCCCTGGCA ACCATGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCACCC 
CCTCTCAACC AGCTAGTGAA GGTGGAGCTG CTGGAGGAAC GGAACACGGC TTTGGGAGTT 
ATCAGTAACT GGACAGACGA GCATCGCATC CCACCCTGCA CCATCTTCCA GGCCTTCAAG 
TACTACCTGG ACATCACCAC GCCTCCAACG CCACTGCAGC TGCAGCAGTT TGCCTCCCTG 
GCCACCAGTG AGAAAGAGAA GCAGCGCCTG CTCGTCCTTA GCAAGGGTCT GCAGGAGTAC 
GAGGAATGGA AATGGGGCAA GAACCCCACC ATTGTGGAGG TGCTGGAGGA GTTCCCATCC 
ATCCAGATGC CCTCTACCCT GCTCCTGACC CAGCTGTCCC TGCTCCAGCC CCGCTACTAT 
TCCATCAGCT CCTCCCCGGA CATGTACCCC GACGAGGTGC ACCTGACTGT GGCCGTTGTC 
TCCTACCGCA CCCGAGATGG AGAAGGACCA ATTCACCATG GCGTGTGCTC CTCCTGGCTC 
AACCGGATCC AGGCTGACGA GGTGGTGCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC 
CTGCCCCGAA ATCCCCAAGT GCCCTGTATC CTGGTTGGCC CAGGCACTGG CATTGCCCCT 
TTCCGAAGCT TCTGGCAGCA ACGGCAATTC GATATCCAAC ACAAAGGAAT GAGTCCCTGC 
CCCATGGTCC TGGTTTTCGG GTGCCGGCAG TCCAAGATAG ACCACATCTA CAAGGAGGAG 
ACCCTGCAGG CCAAGAGCAA GGGGGTCTTC CGAGAACTGT ACACAGCCTA CTCCCGGGAG 
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC CGAGGCTGTG 
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATTTACGTCT GTGGGGACGT CACCATGGCC 
GCCGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAACT CTCCGTAGAG 
GACGCTGGCG TGTTCATCAG CAGGCTGAGG GATGACAACC GGTACCATGA AGATATTTTC 
GGAGTCACCC TGCGCACGTA TGAAGTGACC AACCGCCTTA GATCTGAATC CATTGCCTTC 
ATTGAGGAGA GCAAAAAAGA CACAGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_013990334.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013845788.1 Sus scrofa nitric oxide synthase 1 (neuronal) (NOS1), mRNA.

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CloneID	OSe42808
Clone ID Related Accession (Same CDS sequence)	XM_013990334.1
Accession Version	XM_013990334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	nitric oxide synthase, brain
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003536215.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGACA GAGGACAGAG 
CAGGGTCCCT CCCCTTCCAA CAGCTGCTCC ATGGGCCCTG GGGACCTGGG CACAGATGTG 
ATCCATTCTG AACCTCATGC ATTCATTGGC CTGGGTGATA CTTTCTCCAG GAGGCTCCCA 
AAGTGCCAGC CCCATCCTGA CGTGTGCCCC TCTCTCCACC GCAGATCTGC CATCACCATA 
TTTCCCCAGA GGACTGACGG CAAGCATGAC TTCCGAGTCT GGAACTCCCA GCTCATCCGC 
TACGCCGGCT ACAAGCAGCC CGATGGCTCC ATCCTGGGGG ATCCAGCCAA TGTGGCATTC 
ACGGAGATCT GTATCCAGCA GGGCTGGAAG CCCCCAAGGA GCCGCTTCGA TGTCCTGCCA 
CTCCTGCTCC AGGCCAATGG CAACGACCCT GAGCTCTTCC AGATCCCTCC AGAGCTGGTG 
TTGGAAGTGC CCATCAGACA CCCCAAGTTT GAGTGGTTCA AGGACCTTGG GCTGAAGTGG 
TACGGCCTCC CTGCTGTGTC CAACATGCTA CTGGAGATCG GCGGCCTGGA GTTCAGTGCC 
TGCCCCTTCA GCGGCTGGTA CATGGGCACG GAGATTGGCG TCCGTGACTA CTGTGACAGC 
TCTCGATACA ACATCCTGGA GGAAGTGGCC AAGAAGATGA ACTTGGACAT GAGGAAGACA 
TCCTCCCTGT GGAAGGACCA AGCACTGGTG GAGATCAACA TTGCGGTTCT GTACAGCTTC 
CAGAGTGACA AAGTGACCAT CGTGGACCAC CACTCTGCCA CCGAGTCCTT CATTAAGCAC 
ATGGAGAATG AGTACCGCTG CCGGGGCGGC TGCCCTGCCG ACTGGGTGTG GATCGTGCCC 
CCCATGTCCG GCAGCATCAC CCCTGTCTTC CACCAGGAGA TGCTCAACTA CCGGCTTACC 
CCCTCCTTTG AATACCAGCC TGACCCCTGG AACACCCACG TCTGGAAAGG CACCAACGGG 
ACCCCCACAA AACGGCGAGC CATCGGCTTC AAGAAGCTGG CAGAAGCCGT CAAGTTCTCA 
GCCAAGCTGA TGGGACAGGC TATGGCCAAG AGGGTCAAGG CTACCATCCT TTATGCCACA 
GAGACGGGCA AATCACAGGC TTATGCTAAA ACCTTGTGTG AGATCTTCAA ACATGCCTTT 
GATGCCAAGG TGATGTCCAT GGAAGAATAT GACATCGTAC ACCTGGAACA TGAAACTCTG 
GTCCTAGTGG TTACCAGCAC CTTTGGCAAT GGAGACCCCC CTGAGAATGG GGAGAAGTTT 
GGCTGCGCTT TGATGGAAAT GAGGCACCCC AACTCTGTGC ACGAGGAAAG GAAGTACCCG 
GAACCCTTGC GTTTCTTTCC CCGTAAAGGG CCTTCCCTCG CCCGAGGTTA CACTGAACTG 
CGCGGTCTGG CTGCAGCTCG TGACAGCCAG CACAGGAGCT ACAAGGTGCG ATTCAATAGT 
GTTTCCTCAT ACTCCGACTC CCGCAAATCA TCAGGGGATG GTCCAGACCT CAGAGACAAC 
TTTGAGAGCG CCGGGCCCCT GGCAAATGTG AGGCTCTTCC AGGAGGGTGT CCACGGCGTG 
TCCAAAGGCG CAGAAGTGAG GAGGTCACCA AAAGCCCCGT GTGCGCCACA GGCAGCCTGT 
GATGTGTTCT GCGTGGGAGA CGACGTCAAC ATTGAGAAGG CGAACAACTC CCTCATCAGC 
AATGACCGCA GCTGGAAGAG GAACAAGTTC CGCCTCAGCT ATGTGGCCGA AGCTCCAGAA 
CTGACCCAAG GTCTCTCCAA TGTCCACAAA AAGCGGGTCT CGGCCGCTCG ACTCCTCAGC 
CGTCAAAATC TTCAGAGTCC TAAATCCAGT CAATATGTCA AATGGCGATA CCTCTACAGC 
CTTAATCCCA CTCAGAGGAG TAAGGAGAGA CACCACGAGA GACAAAATAA ACACATAGCT 
TACGCTCAGT TGGTGGCTCA CTGTACAGAA TGTCAAGAGG CTGTCTCGTG CTATTTTAAT 
ATGTGTTTAT GCTTGATTTT TTGTACCAGT TTGCTCAATA GATTTTGGAC ATGCTCCCTG 
CATATGAGAT GGTCACACAA TTCCCAGGAC CCTGGGTGTA GAGGGAGATG GAGAGGTTGC 
TTGGTTCTGG GGCATTCCAG CTCTCCAGGC CCTGTCCTCC ACCCCATGAG CAGGTCTGGC 
CTCCTTGTCT TGGACCCAAG CCCTCAGAGC CCAGACTCCC CAACCCCCGA CCCCCACCAA 
CCCATCTCTC ACCTCCACTG CACCTTCACA GGAGTTATCA GTAACTGGAC AGACGAGCAT 
CGCATCCCAC CCTGCACCAT CTTCCAGGCC TTCAAGTACT ACCTGGACAT CACCACGCCT 
CCAACGCCAC TGCAGCTGCA GCAGTTTGCC TCCCTGGCCA CCAGTGAGAA AGAGAAGCAG 
CGCCTGCTCG TCCTTAGCAA GGGTCTGCAG GAGTACGAGG AATGGAAATG GGGCAAGAAC 
CCCACCATTG TGGAGGTGCT GGAGGAGTTC CCATCCATCC AGATGCCCTC TACCCTGCTC 
CTGACCCAGC TGTCCCTGCT CCAGCCCCGC TACTATTCCA TCAGCTCCTC CCCGGACATG 
TACCCCGACG AGGTGCACCT GACTGTGGCC GTTGTCTCCT ACCGCACCCG AGATGGAGAA 
GGACCAATTC ACCATGGCGT GTGCTCCTCC TGGCTCAACC GGATCCAGGC TGACGAGGTG 
GTGCCCTGTT TCGTGAGAGG AGCACCCAGC TTCCACCTGC CCCGAAATCC CCAAGTGCCC 
TGTATCCTGG TTGGCCCAGG CACTGGCATT GCCCCTTTCC GAAGCTTCTG GCAGCAACGG 
CAATTCGATA TCCAACACAA AGGAATGAGT CCCTGCCCCA TGGTCCTGGT TTTCGGGTGC 
CGGCAGTCCA AGATAGACCA CATCTACAAG GAGGAGACCC TGCAGGCCAA GAGCAAGGGG 
GTCTTCCGAG AACTGTACAC AGCCTACTCC CGGGAGCCAG ACAAACCAAA GAAGTACGTG 
CAGGACATCC TGCAGGAGCA GCTGGCCGAG GCTGTGTACC GAGCCCTGAA GGAGCAAGGG 
GGCCACATTT ACGTCTGTGG GGACGTCACC ATGGCCGCCG ATGTCCTCAA AGCCATCCAG 
CGCATCATGA CCCAGCAGGG GAAACTCTCC GTAGAGGACG CTGGCGTGTT CATCAGCAGG 
CTGAGGGATG ACAACCGGTA CCATGAAGAT ATTTTCGGAG TCACCCTGCG CACGTATGAA 
GTGACCAACC GCCTTAGATC TGAATCCATT GCCTTCATTG AGGAGAGCAA AAAAGACACA 
GATGAGGTTT TCAGCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013845788.1
CDS	663..5123
Translation	MENHMFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTAAVDLSHQSAAGKEQTVVVDGATGPGNGPQHIPDHGQEAGSLSHTNGLAPRPPSQDPAKKSAAVTLQGSGENNKLLKEIEPVLNLLSSGSKGINRGEPAKAETKDVEIQVDRDMDGKSHKTLPLGVENDRVFNDLWGKGNMPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLSDTLHLKSTLGTGCTEHICMGSVMLPSQHIRRPEDVHTKEQLFPLAKEFIDQYYSSIKRQRTEQGPSPSNSCSMGPGDLGTDVIHSEPHAFIGLGDTFSRRLPKCQPHPDVCPSLHRRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVAFTEICIQQGWKPPRSRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDSSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVHEERKYPEPLRFFPRKGPSLARGYTELRGLAAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESAGPLANVRLFQEGVHGVSKGAEVRRSPKAPCAPQAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLSYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSQYVKWRYLYSLNPTQRSKERHHERQNKHIAYAQLVAHCTECQEAVSCYFNMCLCLIFCTSLLNRFWTCSLHMRWSHNSQDPGCRGRWRGCLVLGHSSSPGPVLHPMSRSGLLVLDPSPQSPDSPTPDPHQPISHLHCTFTGVISNWTDEHRIPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPSTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQADEVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYKEETLQAKSKGVFRELYTAYSREPDKPKKYVQDILQEQLAEAVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSVEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_013990334.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa nitric oxide synthase 1 (neuronal) (NOS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013990334.1

ORF Insert Sequence:

ATGGAGAACC ACATGTTCAG CGTTCAGCAG ATCCAGCCCA ATGTCATTTC TGTCCGCCTC 
TTCAAGCGCA AAGTGGGGGG CCTGGGATTC CTGGTGAAGG AAAGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACTTGAT TCGAGGGGGT GCCGCAGAGC AGAGTGGCCT CATCCAGGCT 
GGGGACATCA TTCTTGCGGT CAACGGCCAG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGAGGTAT CGCCTCCGAG ACCCATGTGG TCCTTATTCT GAGGGGCCCT 
GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACCGGGG ATGGGACCCC CAAGACCATC 
CGGGTGACAC GGCCCCTGGC TCCCCCCACC GCAGCAGTTG ATTTGTCCCA CCAATCAGCA 
GCAGGCAAAG AGCAGACCGT GGTGGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG 
CACATACCAG ACCATGGGCA GGAAGCTGGC TCCCTCTCCC ACACCAATGG CTTGGCTCCC 
AGGCCCCCAA GCCAGGACCC TGCCAAGAAA AGTGCTGCGG TCACCCTCCA GGGCAGCGGG 
GAGAACAATA AACTGCTCAA AGAGATAGAG CCCGTGCTGA ATCTTCTCAG CAGTGGGAGC 
AAAGGGATCA ACCGAGGGGA ACCTGCCAAG GCGGAGACAA AGGACGTGGA AATACAGGTG 
GACAGAGATA TGGATGGCAA ATCTCACAAA ACTCTGCCCC TCGGTGTAGA GAACGACCGA 
GTCTTCAATG ACCTATGGGG GAAGGGCAAC ATGCCTGTCG TCCTCAACAA CCCATATTCA 
GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCTCCCA CCAAGAATGG CAGCCCGTCC 
AAGTGCCCCC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG ACGTGGTTCT GTCTGACACC 
CTCCACCTTA AGAGCACATT GGGAACAGGA TGCACTGAGC ACATCTGTAT GGGGTCCGTC 
ATGCTTCCTT CTCAGCACAT ACGAAGGCCC GAAGATGTCC ACACAAAAGA ACAGCTCTTT 
CCTCTCGCCA AAGAGTTCAT TGATCAGTAT TACTCGTCAA TTAAAAGACA GAGGACAGAG 
CAGGGTCCCT CCCCTTCCAA CAGCTGCTCC ATGGGCCCTG GGGACCTGGG CACAGATGTG 
ATCCATTCTG AACCTCATGC ATTCATTGGC CTGGGTGATA CTTTCTCCAG GAGGCTCCCA 
AAGTGCCAGC CCCATCCTGA CGTGTGCCCC TCTCTCCACC GCAGATCTGC CATCACCATA 
TTTCCCCAGA GGACTGACGG CAAGCATGAC TTCCGAGTCT GGAACTCCCA GCTCATCCGC 
TACGCCGGCT ACAAGCAGCC CGATGGCTCC ATCCTGGGGG ATCCAGCCAA TGTGGCATTC 
ACGGAGATCT GTATCCAGCA GGGCTGGAAG CCCCCAAGGA GCCGCTTCGA TGTCCTGCCA 
CTCCTGCTCC AGGCCAATGG CAACGACCCT GAGCTCTTCC AGATCCCTCC AGAGCTGGTG 
TTGGAAGTGC CCATCAGACA CCCCAAGTTT GAGTGGTTCA AGGACCTTGG GCTGAAGTGG 
TACGGCCTCC CTGCTGTGTC CAACATGCTA CTGGAGATCG GCGGCCTGGA GTTCAGTGCC 
TGCCCCTTCA GCGGCTGGTA CATGGGCACG GAGATTGGCG TCCGTGACTA CTGTGACAGC 
TCTCGATACA ACATCCTGGA GGAAGTGGCC AAGAAGATGA ACTTGGACAT GAGGAAGACA 
TCCTCCCTGT GGAAGGACCA AGCACTGGTG GAGATCAACA TTGCGGTTCT GTACAGCTTC 
CAGAGTGACA AAGTGACCAT CGTGGACCAC CACTCTGCCA CCGAGTCCTT CATTAAGCAC 
ATGGAGAATG AGTACCGCTG CCGGGGCGGC TGCCCTGCCG ACTGGGTGTG GATCGTGCCC 
CCCATGTCCG GCAGCATCAC CCCTGTCTTC CACCAGGAGA TGCTCAACTA CCGGCTTACC 
CCCTCCTTTG AATACCAGCC TGACCCCTGG AACACCCACG TCTGGAAAGG CACCAACGGG 
ACCCCCACAA AACGGCGAGC CATCGGCTTC AAGAAGCTGG CAGAAGCCGT CAAGTTCTCA 
GCCAAGCTGA TGGGACAGGC TATGGCCAAG AGGGTCAAGG CTACCATCCT TTATGCCACA 
GAGACGGGCA AATCACAGGC TTATGCTAAA ACCTTGTGTG AGATCTTCAA ACATGCCTTT 
GATGCCAAGG TGATGTCCAT GGAAGAATAT GACATCGTAC ACCTGGAACA TGAAACTCTG 
GTCCTAGTGG TTACCAGCAC CTTTGGCAAT GGAGACCCCC CTGAGAATGG GGAGAAGTTT 
GGCTGCGCTT TGATGGAAAT GAGGCACCCC AACTCTGTGC ACGAGGAAAG GAAGTACCCG 
GAACCCTTGC GTTTCTTTCC CCGTAAAGGG CCTTCCCTCG CCCGAGGTTA CACTGAACTG 
CGCGGTCTGG CTGCAGCTCG TGACAGCCAG CACAGGAGCT ACAAGGTGCG ATTCAATAGT 
GTTTCCTCAT ACTCCGACTC CCGCAAATCA TCAGGGGATG GTCCAGACCT CAGAGACAAC 
TTTGAGAGCG CCGGGCCCCT GGCAAATGTG AGGCTCTTCC AGGAGGGTGT CCACGGCGTG 
TCCAAAGGCG CAGAAGTGAG GAGGTCACCA AAAGCCCCGT GTGCGCCACA GGCAGCCTGT 
GATGTGTTCT GCGTGGGAGA CGACGTCAAC ATTGAGAAGG CGAACAACTC CCTCATCAGC 
AATGACCGCA GCTGGAAGAG GAACAAGTTC CGCCTCAGCT ATGTGGCCGA AGCTCCAGAA 
CTGACCCAAG GTCTCTCCAA TGTCCACAAA AAGCGGGTCT CGGCCGCTCG ACTCCTCAGC 
CGTCAAAATC TTCAGAGTCC TAAATCCAGT CAATATGTCA AATGGCGATA CCTCTACAGC 
CTTAATCCCA CTCAGAGGAG TAAGGAGAGA CACCACGAGA GACAAAATAA ACACATAGCT 
TACGCTCAGT TGGTGGCTCA CTGTACAGAA TGTCAAGAGG CTGTCTCGTG CTATTTTAAT 
ATGTGTTTAT GCTTGATTTT TTGTACCAGT TTGCTCAATA GATTTTGGAC ATGCTCCCTG 
CATATGAGAT GGTCACACAA TTCCCAGGAC CCTGGGTGTA GAGGGAGATG GAGAGGTTGC 
TTGGTTCTGG GGCATTCCAG CTCTCCAGGC CCTGTCCTCC ACCCCATGAG CAGGTCTGGC 
CTCCTTGTCT TGGACCCAAG CCCTCAGAGC CCAGACTCCC CAACCCCCGA CCCCCACCAA 
CCCATCTCTC ACCTCCACTG CACCTTCACA GGAGTTATCA GTAACTGGAC AGACGAGCAT 
CGCATCCCAC CCTGCACCAT CTTCCAGGCC TTCAAGTACT ACCTGGACAT CACCACGCCT 
CCAACGCCAC TGCAGCTGCA GCAGTTTGCC TCCCTGGCCA CCAGTGAGAA AGAGAAGCAG 
CGCCTGCTCG TCCTTAGCAA GGGTCTGCAG GAGTACGAGG AATGGAAATG GGGCAAGAAC 
CCCACCATTG TGGAGGTGCT GGAGGAGTTC CCATCCATCC AGATGCCCTC TACCCTGCTC 
CTGACCCAGC TGTCCCTGCT CCAGCCCCGC TACTATTCCA TCAGCTCCTC CCCGGACATG 
TACCCCGACG AGGTGCACCT GACTGTGGCC GTTGTCTCCT ACCGCACCCG AGATGGAGAA 
GGACCAATTC ACCATGGCGT GTGCTCCTCC TGGCTCAACC GGATCCAGGC TGACGAGGTG 
GTGCCCTGTT TCGTGAGAGG AGCACCCAGC TTCCACCTGC CCCGAAATCC CCAAGTGCCC 
TGTATCCTGG TTGGCCCAGG CACTGGCATT GCCCCTTTCC GAAGCTTCTG GCAGCAACGG 
CAATTCGATA TCCAACACAA AGGAATGAGT CCCTGCCCCA TGGTCCTGGT TTTCGGGTGC 
CGGCAGTCCA AGATAGACCA CATCTACAAG GAGGAGACCC TGCAGGCCAA GAGCAAGGGG 
GTCTTCCGAG AACTGTACAC AGCCTACTCC CGGGAGCCAG ACAAACCAAA GAAGTACGTG 
CAGGACATCC TGCAGGAGCA GCTGGCCGAG GCTGTGTACC GAGCCCTGAA GGAGCAAGGG 
GGCCACATTT ACGTCTGTGG GGACGTCACC ATGGCCGCCG ATGTCCTCAA AGCCATCCAG 
CGCATCATGA CCCAGCAGGG GAAACTCTCC GTAGAGGACG CTGGCGTGTT CATCAGCAGG 
CTGAGGGATG ACAACCGGTA CCATGAAGAT ATTTTCGGAG TCACCCTGCG CACGTATGAA 
GTGACCAACC GCCTTAGATC TGAATCCATT GCCTTCATTG AGGAGAGCAA AAAAGACACA 
GATGAGGTTT TCAGCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NOS1 cDNA ORF clone, Sus scrofa(pig)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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