ORF » Species Summary » Schizosaccharomyces pombe » bub3 cDNA ORF clone

bub3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following bub3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bub3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc102616 NM_001019227.2
Latest version!		 Schizosaccharomyces pombe mitotic spindle checkpoint protein Bub3 (bub3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    bub3 ( NM_001019227.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593798.1 Schizosaccharomyces pombe mitotic spindle checkpoint protein Bub3 (bub3), mRNA.
    Price & Availability↑
    CloneID OSc102616
    Clone ID Related Accession (Same CDS sequence) NM_001019227.2
    Accession Version NM_001019227.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1522598400000
    Organism Schizosaccharomyces pombe(fission yeast)
    Product mitotic spindle checkpoint protein Bub3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019227.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATTTCT CAAAAACATT ACTAAAAAAT TCAAAAGACG GGATTTCAAG TGTAATATTT 
    TCACCCTCTG TCAAAAACGA ACTAATTGCA GGGTGCTGGG ATGGTAGCTT ACTGCATTAT 
    CAAATCTCGG AAAATCCCGA ACTTTTAGGG AAATACGATT TATCAAGTCC TATTCTTTCT 
    TTAGAATATA CAGATGAAAA AACTGCTTTA GTAGGAAATT TAGATGGGAC AGTTACTACC 
    CTCGATCTTA ATACGCGAAA CCATGAGTTT TTAGGAAACC ACGGTAAGGG GGTATCGTGC 
    ATTTCTAAGT TGCGTTTGGA AAACTGTTTC ATTAGTGGGT CTTGGGACAA ATCATTTCGT 
    GTTTGGGATG TTCGCGTAAA GCAACCTGTC GAGGGACAGG ATATAGGAAA AAAAATTTTT 
    GCAAGTAGCT CGAGAGATAA TATATTGGTT CTAGGCTGTT CAGAAAGAGA AAATTTGGTG 
    TACGATATTC GAAATTTAAA ACTTCCATTT CAGCGACGTC CTTCTAGTTT TAAGTATATG 
    ACTCGAAGTG TTTGCTGTAA CCAAAATTTT GAGGGATTCG TTTCTTCCAG CATTGAGGGA 
    AGGACATCTG TTGAATATAT TAACCCGTCT CAGGAAGCGC AAAGCAAAAA TTTTACCTTT 
    AAATGCCATC GGCAGATACA AAAGGACTAC GATATTGTTT ATCCAGTTAA CGACCTAAAA 
    TTTCATCCGA TTCATCAAAC ATTGGCAACT GCTGGGGGAG ATGGCGTTGT TGCGTTTTGG 
    GATATACAAG TCCGAAAACG ATTAAGAGTG TTAAATCCTT CAAAGATCAA TATATCATCT 
    ATTTCTTTTA ATGTTGACGG AAGCATGCTT GCAATTGCCA CTTGTGCTCA AGAAGAAGCC 
    GCTGGTAATA TCTATGTTCA TGCTTTGGAA TCCAACTTTG CAGCTCCAAA GTTAAAGTCA 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_593798.1
    CDS288..1250
    Translation

    Target ORF information:

    RefSeq Version NM_001019227.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe mitotic spindle checkpoint protein Bub3 (bub3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001019227.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATTTCT CAAAAACATT ACTAAAAAAT TCAAAAGACG GGATTTCAAG TGTAATATTT 
    TCACCCTCTG TCAAAAACGA ACTAATTGCA GGGTGCTGGG ATGGTAGCTT ACTGCATTAT 
    CAAATCTCGG AAAATCCCGA ACTTTTAGGG AAATACGATT TATCAAGTCC TATTCTTTCT 
    TTAGAATATA CAGATGAAAA AACTGCTTTA GTAGGAAATT TAGATGGGAC AGTTACTACC 
    CTCGATCTTA ATACGCGAAA CCATGAGTTT TTAGGAAACC ACGGTAAGGG GGTATCGTGC 
    ATTTCTAAGT TGCGTTTGGA AAACTGTTTC ATTAGTGGGT CTTGGGACAA ATCATTTCGT 
    GTTTGGGATG TTCGCGTAAA GCAACCTGTC GAGGGACAGG ATATAGGAAA AAAAATTTTT 
    GCAAGTAGCT CGAGAGATAA TATATTGGTT CTAGGCTGTT CAGAAAGAGA AAATTTGGTG 
    TACGATATTC GAAATTTAAA ACTTCCATTT CAGCGACGTC CTTCTAGTTT TAAGTATATG 
    ACTCGAAGTG TTTGCTGTAA CCAAAATTTT GAGGGATTCG TTTCTTCCAG CATTGAGGGA 
    AGGACATCTG TTGAATATAT TAACCCGTCT CAGGAAGCGC AAAGCAAAAA TTTTACCTTT 
    AAATGCCATC GGCAGATACA AAAGGACTAC GATATTGTTT ATCCAGTTAA CGACCTAAAA 
    TTTCATCCGA TTCATCAAAC ATTGGCAACT GCTGGGGGAG ATGGCGTTGT TGCGTTTTGG 
    GATATACAAG TCCGAAAACG ATTAAGAGTG TTAAATCCTT CAAAGATCAA TATATCATCT 
    ATTTCTTTTA ATGTTGACGG AAGCATGCTT GCAATTGCCA CTTGTGCTCA AGAAGAAGCC 
    GCTGGTAATA TCTATGTTCA TGCTTTGGAA TCCAACTTTG CAGCTCCAAA GTTAAAGTCA 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Schäfer M,Müller-Auer S,Gabel C,Fuchs M,Düsterhöft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dréano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,Sánchez M,del Rey F,Benito J,Domínguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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