GBF1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	GBF1
Entrez Gene ID	100023096
Full Name	golgi brefeldin A resistant guanine nucleotide exchange factor 1
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007479116.2	XP_007479178.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
XM_007479113.2	XP_007479175.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
XM_007479114.2	XP_007479176.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
XM_007479115.2	XP_007479177.1	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following GBF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GBF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc01219	XM_007479113.2 Latest version!	Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc01220	XM_007479114.2 Latest version!	Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc01221	XM_007479115.2 Latest version!	Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMc01222	XM_007479116.2 Latest version!	Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GBF1 ( XM_007479113.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007479175.1 Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMc01219
Clone ID Related Accession (Same CDS sequence)	XM_007479113.2
Accession Version	XM_007479113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007479113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGCC TAGTGGAAGA GAATTCAGTG GTGGCTCACA GGTTTGCCAG GATGGTGGAT 
AAGAGTATTT ACATTATCCA AGGAGAGATT AACATTGTGG TAGGGGCCAT CAAACGGAAT 
GCAAGATGGA GTACTCATAC CCCTGTGGAT GAAGAACGAG ATCCTTTGCT GCATAGTTTC 
AGTCATCTAA AGGAAGTATT AAATAATATA ACAGATCTCT CCGAGATTGA GCCTAATATA 
TTCCTTCGAC CATTCCTGGA AGTTATTCGC TCTGAAGACA CCACTGGTCC CATCACTGGA 
CTGGCACTCA CCTCCGTCAA CAAATTTCTG TCATACTCCC TCATAGATCC CACTCATGAG 
GGCACAGCAG AAGGAATGGA GAACATGGCA GATGCCGTTA CCCATGCTCG TTTTGTGGGC 
ACAGACCCTG CCAGTGACGA GGTTGTCCTG ATGAAAATCC TTCAGGTTCT TCGGACTCTG 
CTGCTAACCC CAGTAGGTGC CCACCTGACC AACGAATCTG TGTGTGAAAT TATGCAGTCT 
TGCTTTCGGA TTTGCTTTGA GATGAGGCTC AGTGAGTTAT TGAGAAAATC CGCAGAGCAC 
ACTCTCGTCG ACATGGTGCA GCTGCTCTTC ACAAGGTTAC CTCAGTTTAA AGAAGAACCG 
AAGAACTACA TAGGGACAAA CATGAAGAAG CTAAAAATGA GAGCAGGAGG TATGAGTGAT 
TCATCCAAAT GGAAAAAGCA GAAGAGGTCT CCACGTCCAC CACGGCATGT CAGCAAAGTC 
ACAGAGTCAC CCACTCCCAA TGGAGCTACC CTGTCCTCCA ACTTTACAGG TGCTGTGTCC 
TTCATTGAAG CCTCCATGCC TATCTCTTCC ACAAGTTCTG AAGCCACCTC CACAGCTGTT 
AGTCCTTCAA CTGACAGTGG CCTTGACCTC TCCTCCCAGA CCACATCCAA GGAAGACCTC 
ACTGACCTGG AGCAGGCAGG CTCTCTAGGA CCCAACATAG CCATGGTGCC TGTAAACAAT 
GAATTGGGCT CTCCTGAATT GTCTGATCCC CAGCATGAAG TGTCCCAGGT GGAAAAGGTC 
CAGTCAGCAT CTGTGGAGTC CATCCCTGAG GTATTAGAAG AATGTAATTC TCTAGCCGAC 
CATTCAGATT CAGCCTCTGT CCATGACATG GACTATGTCA ACCCTCGGGG TGTTCGTTTT 
ACACAGTCCT CTCAGAAAGA AGGAACAGCA TTGGTCCCCT ATGGCCTCCC TTGTATTCGT 
GAGCTCTTCC GCTTCCTCAT CTCTCTTACC AACCCTCATG ACCGACACAA CTCAGAGGTC 
ATGATCCATA TGGGATTGCA GCTACTGACT GTGGCCCTTG AGTCTGCACC TGTGGCCCAG 
TGCCAGACTT TGTTAGGCCT CATCAAGGAT GAAATGTGCC GTCACCTATT CCAGCTGCTA 
AGTGTGGAGC GACTAAGCCT ATATGCAGCC TCTCTCCGAG TATGCTTCCT GCTCTTTGAG 
AGCATGCGAG AACACCTCAA GTTCCAAATG GAGATGTACA TCAAAAAACT CATGGAGATA 
ATCACTGTGG AGAACCCTAA GATGCCTTAT GAAATGAAGG AGATGGCACT AGAAGCTATA 
GTCCAGCTAT GGAGAATCCC CAGTTTTGTT ACTGAGCTCT ACATAAACTA TGACTGTGAC 
TACTATTGCT CCAACCTCTT TGAAGACCTC ACAAAGCTGC TGTCTAAGAA TGCCTTCCCT 
GTCTCTGGGC AGCTTTATAC AACACACCTG CTCTCTCTTG ATGCACTGTT GACAGTGATT 
GACAGTACGG AGGCCCATTG CCAGGCCAAA GTCTTGAACA ACCTCATCCA GCAGGAGAAG 
AAAGAGGCAG CTAAACCCAG TACTGAGATG ATGGATGGCA CGAAAGAAGT CAGCAGCAGC 
AGTGAACAAG CAGCCAGTGA TGGAAAACAC CCAGGGACAA ACCCCGATCA CCCAGGGCTG 
CATCCACCTG GGGGAGGGCA GTTGCTGACT GAGCAGGGAA AATTGGGCTG CAGTGACCTG 
GAGGAAGGTG GTGATGCAGG TGCTGACAAA AAAATTCCCC GGAAGCCACC TCGGTTTTCC 
TGCCTCCTTC CAAGTCCTCA GGAATTGATT GAGATTAAAA GCAAGAAAAA GCTACTGATC 
ACAGGCACAG AACAGTTCAA CCAGAAACCA AAGAAAGGGA TTCAGTTCTT ACAGGAGAAG 
GGTCTTCTGA CTATCCCCAT GGACAACAGT GAAGTTGCCC AGTGGCTTCG AGAGAACCCT 
AGGCTGGACA AGAAGATGAT TGGAGAGTTT GTGAGTGACC GAAAGAACCT TGATCTGCTG 
GAGAGCTTTG TGGGCACCTT CAGTTTCCAG GGTTTACGAC TAGATGAAGC TCTCCGTCTC 
TATTTGGAGG CCTTTCGCTT GCCAGGAGAA GCACCAGTCA TTCAGAGGTT ACTGGAGGCT 
TTTACAGAAC ACTGGAGGAC ATGTAATGGC TCCCCATTTG CAAATAGTGA TGCCTGCTTT 
GCCCTGGCCT ATGCTGTTAT CATGCTCAAC ACTGACCAGC ATAACCACAA TGTCCGAAAA 
CAGAATGTAC CCATGACCCT GGAGGAATTC CGAAAAAACC TGAAGGGCGT GAACGGAGGA 
AAAGATTTTG AGCAGGACAT CCTAGAGGAC ATGTACCATG CCATCAAGAA TGATGAGATT 
GTGATGCCTG AAGAACAGAC TGGTCTGGTG AGGGAGAACT ATGTATGGAA TGTCCTACTG 
CATCGAGGTG CAACCCCTGA GGGTATATTC CTACTGGTAC CTGCTGGCAG CTATGATCAT 
GACCTCTTCA CCATGACGTG GGGCCCTACT ATTGCTGCCC TCTCCTATGT ATTTGACAAG 
AGCCTAGAGG AAACAATTAT CCAAAAGGCC ATTTCAGGCT TCAGGAAATG TGCCATGATC 
TCCGCCCACT ATGGCCTGAG CGATGTGTTT GACAATCTCA TCATCTCTTT GTGCAAATTT 
ACAGCCCTCA GCAGTGAGTC CATTGAGAAC CTGCCCAGTG TGTTTGGGAG CAACCCTAAA 
GCTCACATTG CAGCCAAGAC TGTTTTCCAT TTGGCCCATC GCCATGGTGA CATCCTTCGA 
GAAGGTTGGA AGAACATCAT GGAAGCCATG CTACAGCTGT TCCGAGCCCA GCTATTGCCC 
AAGGCCATGG TAGAGGTGGA AGACTTTGTG GACCCCAATG GCAAGATCTC TCTGCAGCGG 
GAAGAAACAC CATCTAACCG AGGAGAGTCA ACTGTGTTGA GCTTTGTGAG CTGGTTGACG 
CTGAGTGGTC CTGAGCAATC CAGTATGCGG GGTCCATCCA CTGAGAACCA AGAAGCCAAG 
CGCATGGCCC TGGACTGTAT CAAGCAATGT GACCCAGAAA AGATGATCAC AGAAAGCAAG 
TTCCTCCAGC TGGAGTCATT ACAAGAGTTA ATGAAGGCTC TAATATCAGT GACACCAGAT 
GAAGAGACTT ATGATGAGGA AGATGCAGCA TTCTGCCTAG AGATGCTTCT TCGGATTGTG 
CTAGAGAACA GGGACCGTGT GGGCTGTGTA TGGCAGACAG TGCGTGATCA TCTGTACCAC 
CTCTGTGTCC AGGCGCTGGA TTTCTGCTTC CTGGTGGAAC GGGCTGTGGT GGGGTTACTT 
CGCCTGGCCA TACGGTTGCT AAGGAGAGAG GAAATCAGTG GCCAGGTGCT GCTGTCTCTT 
CGTATCCTGC TACTGATGAA ACCTAGTGTA CTGTCTCGTG TCAGCCACCA GGTTGCCTAT 
GGGCTCCATG AGTTACTCAA GACCAATGCA GCTAACATAC ACTCTGGGGA TGACTGGGCC 
ACACTTTTTA CTTTGCTGGA ATGCATTGGC TCAGGGGTGA AGCCTCCCCC TTCTTTGCAG 
GCCACAGCTA GGACAGAGAC CACTGATACT GGTGCTCAGT CAGACAGTGA ACTCTCTTCC 
TACCATCCCA GCGATGTCAG CTTGGATCGA GGCTACACCT CAGACTCAGA AGTCTATACT 
GATCACGGTA GACCAGGCAA GATGCACCGC TCAGCTACTG ATGCCGACGT GGTCAACAGT 
GGTTGGCTAG TGGTGGGGAA AGATGACATA GACAGCTCCA AGCCAGGACC AGGACCAAAC 
CGGCTTGGCC CTTCCCCTCT TGTGAATCAG TACAGCCTTA CTGTGGGCTT GGACTTGGGA 
CCTCATGACA CAAAGTCCCT CCTCAAATGT GTGGAGTCAC TGTCTTTCAT CGTTCGTGAT 
GCTGCTCATA TTACTCCAGA CAATTTTGAG CTCTGTGTCA AGACCATCCG AATTTTTGTA 
GAGGCCAGTC TGAATGGTGG ATGCAAATCT CAGGAAAAAC GAAGCAAATG CCACAAATAT 
GACAGCAAAG GGAACCGCTT CAAGAAGAAG TCGAAGGATG GCTCAGTTCT TCGTCGACCA 
CGAGTGACCA GCCAGCATAC AATTCGTGGC GGACACAGTG ATGATGAAGA TGATGAAGGA 
GTACCTGCTA GCTACCACAC GGTGTCTTTA CAGGTCAGTC AGGACCTGCT GGACTTGATG 
CACACCCTAC ACACACGGGC AGCAACTATT TACAACTCAT GGGCTGAGGA GCAGCGTCAC 
TTAGAGACAG GGGGCAAGAA GATTGAGGCT GATTCCCGGA CCCTCTGGGC TCATTGCTGG 
TGCCCCTTGC TTCAGGGTAT TGCCTGGCTC TGCTGTGATG CCCGGCGTCA GGTACGGATG 
CAGGCACTCA CCTACCTACA GAGGGCCCTG TTGGTACATG ACCTGCAGAA GCTAGATGCC 
CTGGAATGGG AGTCCTGTTT TAATAAGGTG CTGTTTCCTC TGCTGACCAA GCTTCTAGAG 
AACATCAGTC CTGCAGATGT GGGTGGGATG GAGGAGACAC GGATGAGGGC TTCCACACTG 
CTCTCCAAGG TTTTCCTGCA GCACCTGTCC CCACTGCTGT CACTTCCAAC CTTTGCTGCT 
CTCTGGCTGA CCATCCTGGA CTTCATGGAC AAGTATATGC ATGCAGGTTC CAGTGATTTA 
CTGTCTGAGG CTATCCCTGA GTCATTGAAG AACATGCTGC TAGTGATGGA TACTGCAGAG 
ATCTTCCACA ACGCAGACAC AAGAGGAAGT GGCTCCTCTG CCCTCTGGGA GATCACCTGG 
GAGCGTATTG ACTGCTTCCT GCCCCATCTT CGAGATGAGC TCTTCAAGCA GACAGTCATC 
CCTGATCCCC TGCCACCGGT GCCTACTGAA CCACATTCCC AGAAGACTCT GGCCTCTGCC 
CATTTGTCTC CTGCTGCCAG TGACACAAGG ATGCCCAGCC ACACATATAC TCCAGAAATG 
CCCTCAGAAC TGGGGGCCTA TGACTCAGAG AAGTCAGAGG TCTCTGGAGC AGCTGCCAGT 
GGTTGTTCTG GTTCTTCCAC AGTCTTGACT CCCAGCAAGC TGAGCTCCAG TCCAGAAGGG 
CCTGTGTCAT TGGCCCAGCC CCCACTGATC CTGCAGCCTC TGGCTTCCCC ACTGCAAGTG 
GGTGTGCCAC CCATGACATT ACCTATCATT CTAAATCCAG CCCTCATTGA GGCAACTTCA 
CCTGTGCCTC TGCTAGCTGC CCCTCGCCCC ACTGACTCTA TGCCCACTTC TGAAGTCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007479175.1
CDS	69..5711
Translation	MGGLVEENSVVAHRFARMVDKSIYIIQGEINIVVGAIKRNARWSTHTPVDEERDPLLHSFSHLKEVLNNITDLSEIEPNIFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYSLIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYIGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHVSKVTESPTPNGATLSSNFTGAVSFIEASMPISSTSSEATSTAVSPSTDSGLDLSSQTTSKEDLTDLEQAGSLGPNIAMVPVNNELGSPELSDPQHEVSQVEKVQSASVESIPEVLEECNSLADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLQLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLSLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNNLIQQEKKEAAKPSTEMMDGTKEVSSSSEQAASDGKHPGTNPDHPGLHPPGGGQLLTEQGKLGCSDLEEGGDAGADKKIPRKPPRFSCLLPSPQELIEIKSKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNSEVAQWLRENPRLDKKMIGEFVSDRKNLDLLESFVGTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRTCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNVPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNDEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLLVPAGSYDHDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSMRGPSTENQEAKRMALDCIKQCDPEKMITESKFLQLESLQELMKALISVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQALDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPPSLQATARTETTDTGAQSDSELSSYHPSDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADVVNSGWLVVGKDDIDSSKPGPGPNRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTIRIFVEASLNGGCKSQEKRSKCHKYDSKGNRFKKKSKDGSVLRRPRVTSQHTIRGGHSDDEDDEGVPASYHTVSLQVSQDLLDLMHTLHTRAATIYNSWAEEQRHLETGGKKIEADSRTLWAHCWCPLLQGIAWLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLPTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADTRGSGSSALWEITWERIDCFLPHLRDELFKQTVIPDPLPPVPTEPHSQKTLASAHLSPAASDTRMPSHTYTPEMPSELGAYDSEKSEVSGAAASGCSGSSTVLTPSKLSSSPEGPVSLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLAAPRPTDSMPTSEVN

Target ORF information:

RefSeq Version	XM_007479113.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007479113.2

ORF Insert Sequence:

ATGGGAGGCC TAGTGGAAGA GAATTCAGTG GTGGCTCACA GGTTTGCCAG GATGGTGGAT 
AAGAGTATTT ACATTATCCA AGGAGAGATT AACATTGTGG TAGGGGCCAT CAAACGGAAT 
GCAAGATGGA GTACTCATAC CCCTGTGGAT GAAGAACGAG ATCCTTTGCT GCATAGTTTC 
AGTCATCTAA AGGAAGTATT AAATAATATA ACAGATCTCT CCGAGATTGA GCCTAATATA 
TTCCTTCGAC CATTCCTGGA AGTTATTCGC TCTGAAGACA CCACTGGTCC CATCACTGGA 
CTGGCACTCA CCTCCGTCAA CAAATTTCTG TCATACTCCC TCATAGATCC CACTCATGAG 
GGCACAGCAG AAGGAATGGA GAACATGGCA GATGCCGTTA CCCATGCTCG TTTTGTGGGC 
ACAGACCCTG CCAGTGACGA GGTTGTCCTG ATGAAAATCC TTCAGGTTCT TCGGACTCTG 
CTGCTAACCC CAGTAGGTGC CCACCTGACC AACGAATCTG TGTGTGAAAT TATGCAGTCT 
TGCTTTCGGA TTTGCTTTGA GATGAGGCTC AGTGAGTTAT TGAGAAAATC CGCAGAGCAC 
ACTCTCGTCG ACATGGTGCA GCTGCTCTTC ACAAGGTTAC CTCAGTTTAA AGAAGAACCG 
AAGAACTACA TAGGGACAAA CATGAAGAAG CTAAAAATGA GAGCAGGAGG TATGAGTGAT 
TCATCCAAAT GGAAAAAGCA GAAGAGGTCT CCACGTCCAC CACGGCATGT CAGCAAAGTC 
ACAGAGTCAC CCACTCCCAA TGGAGCTACC CTGTCCTCCA ACTTTACAGG TGCTGTGTCC 
TTCATTGAAG CCTCCATGCC TATCTCTTCC ACAAGTTCTG AAGCCACCTC CACAGCTGTT 
AGTCCTTCAA CTGACAGTGG CCTTGACCTC TCCTCCCAGA CCACATCCAA GGAAGACCTC 
ACTGACCTGG AGCAGGCAGG CTCTCTAGGA CCCAACATAG CCATGGTGCC TGTAAACAAT 
GAATTGGGCT CTCCTGAATT GTCTGATCCC CAGCATGAAG TGTCCCAGGT GGAAAAGGTC 
CAGTCAGCAT CTGTGGAGTC CATCCCTGAG GTATTAGAAG AATGTAATTC TCTAGCCGAC 
CATTCAGATT CAGCCTCTGT CCATGACATG GACTATGTCA ACCCTCGGGG TGTTCGTTTT 
ACACAGTCCT CTCAGAAAGA AGGAACAGCA TTGGTCCCCT ATGGCCTCCC TTGTATTCGT 
GAGCTCTTCC GCTTCCTCAT CTCTCTTACC AACCCTCATG ACCGACACAA CTCAGAGGTC 
ATGATCCATA TGGGATTGCA GCTACTGACT GTGGCCCTTG AGTCTGCACC TGTGGCCCAG 
TGCCAGACTT TGTTAGGCCT CATCAAGGAT GAAATGTGCC GTCACCTATT CCAGCTGCTA 
AGTGTGGAGC GACTAAGCCT ATATGCAGCC TCTCTCCGAG TATGCTTCCT GCTCTTTGAG 
AGCATGCGAG AACACCTCAA GTTCCAAATG GAGATGTACA TCAAAAAACT CATGGAGATA 
ATCACTGTGG AGAACCCTAA GATGCCTTAT GAAATGAAGG AGATGGCACT AGAAGCTATA 
GTCCAGCTAT GGAGAATCCC CAGTTTTGTT ACTGAGCTCT ACATAAACTA TGACTGTGAC 
TACTATTGCT CCAACCTCTT TGAAGACCTC ACAAAGCTGC TGTCTAAGAA TGCCTTCCCT 
GTCTCTGGGC AGCTTTATAC AACACACCTG CTCTCTCTTG ATGCACTGTT GACAGTGATT 
GACAGTACGG AGGCCCATTG CCAGGCCAAA GTCTTGAACA ACCTCATCCA GCAGGAGAAG 
AAAGAGGCAG CTAAACCCAG TACTGAGATG ATGGATGGCA CGAAAGAAGT CAGCAGCAGC 
AGTGAACAAG CAGCCAGTGA TGGAAAACAC CCAGGGACAA ACCCCGATCA CCCAGGGCTG 
CATCCACCTG GGGGAGGGCA GTTGCTGACT GAGCAGGGAA AATTGGGCTG CAGTGACCTG 
GAGGAAGGTG GTGATGCAGG TGCTGACAAA AAAATTCCCC GGAAGCCACC TCGGTTTTCC 
TGCCTCCTTC CAAGTCCTCA GGAATTGATT GAGATTAAAA GCAAGAAAAA GCTACTGATC 
ACAGGCACAG AACAGTTCAA CCAGAAACCA AAGAAAGGGA TTCAGTTCTT ACAGGAGAAG 
GGTCTTCTGA CTATCCCCAT GGACAACAGT GAAGTTGCCC AGTGGCTTCG AGAGAACCCT 
AGGCTGGACA AGAAGATGAT TGGAGAGTTT GTGAGTGACC GAAAGAACCT TGATCTGCTG 
GAGAGCTTTG TGGGCACCTT CAGTTTCCAG GGTTTACGAC TAGATGAAGC TCTCCGTCTC 
TATTTGGAGG CCTTTCGCTT GCCAGGAGAA GCACCAGTCA TTCAGAGGTT ACTGGAGGCT 
TTTACAGAAC ACTGGAGGAC ATGTAATGGC TCCCCATTTG CAAATAGTGA TGCCTGCTTT 
GCCCTGGCCT ATGCTGTTAT CATGCTCAAC ACTGACCAGC ATAACCACAA TGTCCGAAAA 
CAGAATGTAC CCATGACCCT GGAGGAATTC CGAAAAAACC TGAAGGGCGT GAACGGAGGA 
AAAGATTTTG AGCAGGACAT CCTAGAGGAC ATGTACCATG CCATCAAGAA TGATGAGATT 
GTGATGCCTG AAGAACAGAC TGGTCTGGTG AGGGAGAACT ATGTATGGAA TGTCCTACTG 
CATCGAGGTG CAACCCCTGA GGGTATATTC CTACTGGTAC CTGCTGGCAG CTATGATCAT 
GACCTCTTCA CCATGACGTG GGGCCCTACT ATTGCTGCCC TCTCCTATGT ATTTGACAAG 
AGCCTAGAGG AAACAATTAT CCAAAAGGCC ATTTCAGGCT TCAGGAAATG TGCCATGATC 
TCCGCCCACT ATGGCCTGAG CGATGTGTTT GACAATCTCA TCATCTCTTT GTGCAAATTT 
ACAGCCCTCA GCAGTGAGTC CATTGAGAAC CTGCCCAGTG TGTTTGGGAG CAACCCTAAA 
GCTCACATTG CAGCCAAGAC TGTTTTCCAT TTGGCCCATC GCCATGGTGA CATCCTTCGA 
GAAGGTTGGA AGAACATCAT GGAAGCCATG CTACAGCTGT TCCGAGCCCA GCTATTGCCC 
AAGGCCATGG TAGAGGTGGA AGACTTTGTG GACCCCAATG GCAAGATCTC TCTGCAGCGG 
GAAGAAACAC CATCTAACCG AGGAGAGTCA ACTGTGTTGA GCTTTGTGAG CTGGTTGACG 
CTGAGTGGTC CTGAGCAATC CAGTATGCGG GGTCCATCCA CTGAGAACCA AGAAGCCAAG 
CGCATGGCCC TGGACTGTAT CAAGCAATGT GACCCAGAAA AGATGATCAC AGAAAGCAAG 
TTCCTCCAGC TGGAGTCATT ACAAGAGTTA ATGAAGGCTC TAATATCAGT GACACCAGAT 
GAAGAGACTT ATGATGAGGA AGATGCAGCA TTCTGCCTAG AGATGCTTCT TCGGATTGTG 
CTAGAGAACA GGGACCGTGT GGGCTGTGTA TGGCAGACAG TGCGTGATCA TCTGTACCAC 
CTCTGTGTCC AGGCGCTGGA TTTCTGCTTC CTGGTGGAAC GGGCTGTGGT GGGGTTACTT 
CGCCTGGCCA TACGGTTGCT AAGGAGAGAG GAAATCAGTG GCCAGGTGCT GCTGTCTCTT 
CGTATCCTGC TACTGATGAA ACCTAGTGTA CTGTCTCGTG TCAGCCACCA GGTTGCCTAT 
GGGCTCCATG AGTTACTCAA GACCAATGCA GCTAACATAC ACTCTGGGGA TGACTGGGCC 
ACACTTTTTA CTTTGCTGGA ATGCATTGGC TCAGGGGTGA AGCCTCCCCC TTCTTTGCAG 
GCCACAGCTA GGACAGAGAC CACTGATACT GGTGCTCAGT CAGACAGTGA ACTCTCTTCC 
TACCATCCCA GCGATGTCAG CTTGGATCGA GGCTACACCT CAGACTCAGA AGTCTATACT 
GATCACGGTA GACCAGGCAA GATGCACCGC TCAGCTACTG ATGCCGACGT GGTCAACAGT 
GGTTGGCTAG TGGTGGGGAA AGATGACATA GACAGCTCCA AGCCAGGACC AGGACCAAAC 
CGGCTTGGCC CTTCCCCTCT TGTGAATCAG TACAGCCTTA CTGTGGGCTT GGACTTGGGA 
CCTCATGACA CAAAGTCCCT CCTCAAATGT GTGGAGTCAC TGTCTTTCAT CGTTCGTGAT 
GCTGCTCATA TTACTCCAGA CAATTTTGAG CTCTGTGTCA AGACCATCCG AATTTTTGTA 
GAGGCCAGTC TGAATGGTGG ATGCAAATCT CAGGAAAAAC GAAGCAAATG CCACAAATAT 
GACAGCAAAG GGAACCGCTT CAAGAAGAAG TCGAAGGATG GCTCAGTTCT TCGTCGACCA 
CGAGTGACCA GCCAGCATAC AATTCGTGGC GGACACAGTG ATGATGAAGA TGATGAAGGA 
GTACCTGCTA GCTACCACAC GGTGTCTTTA CAGGTCAGTC AGGACCTGCT GGACTTGATG 
CACACCCTAC ACACACGGGC AGCAACTATT TACAACTCAT GGGCTGAGGA GCAGCGTCAC 
TTAGAGACAG GGGGCAAGAA GATTGAGGCT GATTCCCGGA CCCTCTGGGC TCATTGCTGG 
TGCCCCTTGC TTCAGGGTAT TGCCTGGCTC TGCTGTGATG CCCGGCGTCA GGTACGGATG 
CAGGCACTCA CCTACCTACA GAGGGCCCTG TTGGTACATG ACCTGCAGAA GCTAGATGCC 
CTGGAATGGG AGTCCTGTTT TAATAAGGTG CTGTTTCCTC TGCTGACCAA GCTTCTAGAG 
AACATCAGTC CTGCAGATGT GGGTGGGATG GAGGAGACAC GGATGAGGGC TTCCACACTG 
CTCTCCAAGG TTTTCCTGCA GCACCTGTCC CCACTGCTGT CACTTCCAAC CTTTGCTGCT 
CTCTGGCTGA CCATCCTGGA CTTCATGGAC AAGTATATGC ATGCAGGTTC CAGTGATTTA 
CTGTCTGAGG CTATCCCTGA GTCATTGAAG AACATGCTGC TAGTGATGGA TACTGCAGAG 
ATCTTCCACA ACGCAGACAC AAGAGGAAGT GGCTCCTCTG CCCTCTGGGA GATCACCTGG 
GAGCGTATTG ACTGCTTCCT GCCCCATCTT CGAGATGAGC TCTTCAAGCA GACAGTCATC 
CCTGATCCCC TGCCACCGGT GCCTACTGAA CCACATTCCC AGAAGACTCT GGCCTCTGCC 
CATTTGTCTC CTGCTGCCAG TGACACAAGG ATGCCCAGCC ACACATATAC TCCAGAAATG 
CCCTCAGAAC TGGGGGCCTA TGACTCAGAG AAGTCAGAGG TCTCTGGAGC AGCTGCCAGT 
GGTTGTTCTG GTTCTTCCAC AGTCTTGACT CCCAGCAAGC TGAGCTCCAG TCCAGAAGGG 
CCTGTGTCAT TGGCCCAGCC CCCACTGATC CTGCAGCCTC TGGCTTCCCC ACTGCAAGTG 
GGTGTGCCAC CCATGACATT ACCTATCATT CTAAATCCAG CCCTCATTGA GGCAACTTCA 
CCTGTGCCTC TGCTAGCTGC CCCTCGCCCC ACTGACTCTA TGCCCACTTC TGAAGTCAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_007479114.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007479176.1 Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

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CloneID	OMc01220
Clone ID Related Accession (Same CDS sequence)	XM_007479114.2
Accession Version	XM_007479114.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007479114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGCC TAGTGGAAGA GAATTCAGTG GTGGCTCACA GGTTTGCCAG GATGGTGGAT 
AAGAGTATTT ACATTATCCA AGGAGAGATT AACATTGTGG TAGGGGCCAT CAAACGGAAT 
GCAAGATGGA GTACTCATAC CCCTGTGGAT GAAGAACGAG ATCCTTTGCT GCATAGTTTC 
AGTCATCTAA AGGAAGTATT AAATAATATA ACAGATCTCT CCGAGATTGA GCCTAATATA 
TTCCTTCGAC CATTCCTGGA AGTTATTCGC TCTGAAGACA CCACTGGTCC CATCACTGGA 
CTGGCACTCA CCTCCGTCAA CAAATTTCTG TCATACTCCC TCATAGATCC CACTCATGAG 
GGCACAGCAG AAGGAATGGA GAACATGGCA GATGCCGTTA CCCATGCTCG TTTTGTGGGC 
ACAGACCCTG CCAGTGACGA GGTTGTCCTG ATGAAAATCC TTCAGGTTCT TCGGACTCTG 
CTGCTAACCC CAGTAGGTGC CCACCTGACC AACGAATCTG TGTGTGAAAT TATGCAGTCT 
TGCTTTCGGA TTTGCTTTGA GATGAGGCTC AGTGAGTTAT TGAGAAAATC CGCAGAGCAC 
ACTCTCGTCG ACATGGTGCA GCTGCTCTTC ACAAGGTTAC CTCAGTTTAA AGAAGAACCG 
AAGAACTACA TAGGGACAAA CATGAAGAAG CTAAAAATGA GAGCAGGAGG TATGAGTGAT 
TCATCCAAAT GGAAAAAGCA GAAGAGGTCT CCACGTCCAC CACGGCATGT CAGCAAAGTC 
ACAGAGTCAC CCACTCCCAA TGGAGCTACC CTGTCCTCCA ACTTTACAGG TGCTGTGTCC 
TTCATTGAAG CCTCCATGCC TATCTCTTCC ACAAGTTCTG AAGCCACCTC CACAGCTGTT 
AGTCCTTCAA CTGACAGTGG CCTTGACCTC TCCTCCCAGA CCACATCCAA GGAAGACCTC 
ACTGACCTGG AGCAGGCAGG CTCTCTAGGA CCCAACATAG CCATGGTGCC TGTAAACAAT 
GAATTGGGCT CTCCTGAATT GTCTGATCCC CAGCATGAAG TGTCCCAGGT GGAAAAGGTC 
CAGTCAGCAT CTGTGGAGTC CATCCCTGAG GTATTAGAAG AATGTAATTC TCTAGCCGAC 
CATTCAGATT CAGCCTCTGT CCATGACATG GACTATGTCA ACCCTCGGGG TGTTCGTTTT 
ACACAGTCCT CTCAGAAAGA AGGAACAGCA TTGGTCCCCT ATGGCCTCCC TTGTATTCGT 
GAGCTCTTCC GCTTCCTCAT CTCTCTTACC AACCCTCATG ACCGACACAA CTCAGAGGTC 
ATGATCCATA TGGGATTGCA GCTACTGACT GTGGCCCTTG AGTCTGCACC TGTGGCCCAG 
TGCCAGACTT TGTTAGGCCT CATCAAGGAT GAAATGTGCC GTCACCTATT CCAGCTGCTA 
AGTGTGGAGC GACTAAGCCT ATATGCAGCC TCTCTCCGAG TATGCTTCCT GCTCTTTGAG 
AGCATGCGAG AACACCTCAA GTTCCAAATG GAGATGTACA TCAAAAAACT CATGGAGATA 
ATCACTGTGG AGAACCCTAA GATGCCTTAT GAAATGAAGG AGATGGCACT AGAAGCTATA 
GTCCAGCTAT GGAGAATCCC CAGTTTTGTT ACTGAGCTCT ACATAAACTA TGACTGTGAC 
TACTATTGCT CCAACCTCTT TGAAGACCTC ACAAAGCTGC TGTCTAAGAA TGCCTTCCCT 
GTCTCTGGGC AGCTTTATAC AACACACCTG CTCTCTCTTG ATGCACTGTT GACAGTGATT 
GACAGTACGG AGGCCCATTG CCAGGCCAAA GTCTTGAACA ACCTCATCCA GCAGGAGAAG 
AAAGAGGCAG CTAAACCCAG TACTGAGATG ATGGATGGCA CGAAAGAAGT CAGCAGCAGC 
AGTGAACAAG CAGCCAGTGA TGGAAAACAC CCAGGGACAA ACCCCGATCA CCCAGGGCTG 
CATCCACCTG GGGGAGGGCA GTTGCTGACT GAGCAGGGAA AATTGGGCTG CAGTGACCTG 
GAGGAAGGTG GTGATGCAGG TGCTGACAAA AAAATTCCCC GGAAGCCACC TCGGTTTTCC 
TGCCTCCTTC CAAGTCCTCA GGAATTGATT GAGATTAAAA GCAAGAAAAA GCTACTGATC 
ACAGGCACAG AACAGTTCAA CCAGAAACCA AAGAAAGGGA TTCAGTTCTT ACAGGAGAAG 
GGTCTTCTGA CTATCCCCAT GGACAACAGT GAAGTTGCCC AGTGGCTTCG AGAGAACCCT 
AGGCTGGACA AGAAGATGAT TGGAGAGTTT GTGAGTGACC GAAAGAACCT TGATCTGCTG 
GAGAGCTTTG TGGGCACCTT CAGTTTCCAG GGTTTACGAC TAGATGAAGC TCTCCGTCTC 
TATTTGGAGG CCTTTCGCTT GCCAGGAGAA GCACCAGTCA TTCAGAGGTT ACTGGAGGCT 
TTTACAGAAC ACTGGAGGAC ATGTAATGGC TCCCCATTTG CAAATAGTGA TGCCTGCTTT 
GCCCTGGCCT ATGCTGTTAT CATGCTCAAC ACTGACCAGC ATAACCACAA TGTCCGAAAA 
CAGAATGTAC CCATGACCCT GGAGGAATTC CGAAAAAACC TGAAGGGCGT GAACGGAGGA 
AAAGATTTTG AGCAGGACAT CCTAGAGGAC ATGTACCATG CCATCAAGAA TGATGAGATT 
GTGATGCCTG AAGAACAGAC TGGTCTGGTG AGGGAGAACT ATGTATGGAA TGTCCTACTG 
CATCGAGGTG CAACCCCTGA GGGTATATTC CTACTGGTAC CTGCTGGCAG CTATGATCAT 
GACCTCTTCA CCATGACGTG GGGCCCTACT ATTGCTGCCC TCTCCTATGT ATTTGACAAG 
AGCCTAGAGG AAACAATTAT CCAAAAGGCC ATTTCAGGCT TCAGGAAATG TGCCATGATC 
TCCGCCCACT ATGGCCTGAG CGATGTGTTT GACAATCTCA TCATCTCTTT GTGCAAATTT 
ACAGCCCTCA GCAGTGAGTC CATTGAGAAC CTGCCCAGTG TGTTTGGGAG CAACCCTAAA 
GCTCACATTG CAGCCAAGAC TGTTTTCCAT TTGGCCCATC GCCATGGTGA CATCCTTCGA 
GAAGGTTGGA AGAACATCAT GGAAGCCATG CTACAGCTGT TCCGAGCCCA GCTATTGCCC 
AAGGCCATGG TAGAGGTGGA AGACTTTGTG GACCCCAATG GCAAGATCTC TCTGCAGCGG 
GAAGAAACAC CATCTAACCG AGGAGAGTCA ACTGTGTTGA GCTTTGTGAG CTGGTTGACG 
CTGAGTGGTC CTGAGCAATC CAGTATGCGG GGTCCATCCA CTGAGAACCA AGAAGCCAAG 
CGCATGGCCC TGGACTGTAT CAAGCAATGT GACCCAGAAA AGATGATCAC AGAAAGCAAG 
TTCCTCCAGC TGGAGTCATT ACAAGAGTTA ATGAAGGCTC TAATATCAGT GACACCAGAT 
GAAGAGACTT ATGATGAGGA AGATGCAGCA TTCTGCCTAG AGATGCTTCT TCGGATTGTG 
CTAGAGAACA GGGACCGTGT GGGCTGTGTA TGGCAGACAG TGCGTGATCA TCTGTACCAC 
CTCTGTGTCC AGGCGCTGGA TTTCTGCTTC CTGGTGGAAC GGGCTGTGGT GGGGTTACTT 
CGCCTGGCCA TACGGTTGCT AAGGAGAGAG GAAATCAGTG GCCAGGTGCT GCTGTCTCTT 
CGTATCCTGC TACTGATGAA ACCTAGTGTA CTGTCTCGTG TCAGCCACCA GGTTGCCTAT 
GGGCTCCATG AGTTACTCAA GACCAATGCA GCTAACATAC ACTCTGGGGA TGACTGGGCC 
ACACTTTTTA CTTTGCTGGA ATGCATTGGC TCAGGGGTGA AGCCTCCCCC TTCTTTGCAG 
GCCACAGCTA GGACAGAGAC CACTGATACT GGTGCTCAGT CAGACAGTGA ACTCTCTTCC 
TACCATCCCA GCGATGTCAG CTTGGATCGA GGCTACACCT CAGACTCAGA AGTCTATACT 
GATCACGGTA GACCAGGCAA GATGCACCGC TCAGCTACTG ATGCCGACGT GGTCAACAGT 
GGTTGGCTAG TGGTGGGGAA AGATGACATA GACAGCTCCA AGCCAGGACC AGGACCAAAC 
CGGCTTGGCC CTTCCCCTCT TGTGAATCAG TACAGCCTTA CTGTGGGCTT GGACTTGGGA 
CCTCATGACA CAAAGTCCCT CCTCAAATGT GTGGAGTCAC TGTCTTTCAT CGTTCGTGAT 
GCTGCTCATA TTACTCCAGA CAATTTTGAG CTCTGTGTCA AGACCATCCG AATTTTTGTA 
GAGGCCAGTC TGAATGGTGG ATGCAAATCT CAGGAAAAAC GAAGCAAATG CCACAAATAT 
GACAGCAAAG GGAACCGCTT CAAGAAGAAG TCGAAGGATG GCTCAGTTCT TCGTCGACCA 
CGAGTGACCA GCCAGCATAC AATTCGTGGC GGACACAGTG ATGATGAAGA TGATGAAGGA 
GTACCTGCTA GCTACCACAC GGTGTCTTTA CAGCTGCTGG ACTTGATGCA CACCCTACAC 
ACACGGGCAG CAACTATTTA CAACTCATGG GCTGAGGAGC AGCGTCACTT AGAGACAGGG 
GGCAAGAAGA TTGAGGCTGA TTCCCGGACC CTCTGGGCTC ATTGCTGGTG CCCCTTGCTT 
CAGGGTATTG CCTGGCTCTG CTGTGATGCC CGGCGTCAGG TACGGATGCA GGCACTCACC 
TACCTACAGA GGGCCCTGTT GGTACATGAC CTGCAGAAGC TAGATGCCCT GGAATGGGAG 
TCCTGTTTTA ATAAGGTGCT GTTTCCTCTG CTGACCAAGC TTCTAGAGAA CATCAGTCCT 
GCAGATGTGG GTGGGATGGA GGAGACACGG ATGAGGGCTT CCACACTGCT CTCCAAGGTT 
TTCCTGCAGC ACCTGTCCCC ACTGCTGTCA CTTCCAACCT TTGCTGCTCT CTGGCTGACC 
ATCCTGGACT TCATGGACAA GTATATGCAT GCAGGTTCCA GTGATTTACT GTCTGAGGCT 
ATCCCTGAGT CATTGAAGAA CATGCTGCTA GTGATGGATA CTGCAGAGAT CTTCCACAAC 
GCAGACACAA GAGGAAGTGG CTCCTCTGCC CTCTGGGAGA TCACCTGGGA GCGTATTGAC 
TGCTTCCTGC CCCATCTTCG AGATGAGCTC TTCAAGCAGA CAGTCATCCC TGATCCCCTG 
CCACCGGTGC CTACTGAACC ACATTCCCAG AAGACTCTGG CCTCTGCCCA TTTGTCTCCT 
GCTGCCAGTG ACACAAGGAT GCCCAGCCAC ACATATACTC CAGAAATGCC CTCAGAACTG 
GGGGCCTATG ACTCAGAGAA GTCAGAGGTC TCTGGAGCAG CTGCCAGTGG TTGTTCTGGT 
TCTTCCACAG TCTTGACTCC CAGCAAGCTG AGCTCCAGTC CAGAAGGGCC TGTGTCATTG 
GCCCAGCCCC CACTGATCCT GCAGCCTCTG GCTTCCCCAC TGCAAGTGGG TGTGCCACCC 
ATGACATTAC CTATCATTCT AAATCCAGCC CTCATTGAGG CAACTTCACC TGTGCCTCTG 
CTAGCTGCCC CTCGCCCCAC TGACTCTATG CCCACTTCTG AAGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007479176.1
CDS	69..5699
Translation	MGGLVEENSVVAHRFARMVDKSIYIIQGEINIVVGAIKRNARWSTHTPVDEERDPLLHSFSHLKEVLNNITDLSEIEPNIFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYSLIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYIGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHVSKVTESPTPNGATLSSNFTGAVSFIEASMPISSTSSEATSTAVSPSTDSGLDLSSQTTSKEDLTDLEQAGSLGPNIAMVPVNNELGSPELSDPQHEVSQVEKVQSASVESIPEVLEECNSLADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLQLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLSLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNNLIQQEKKEAAKPSTEMMDGTKEVSSSSEQAASDGKHPGTNPDHPGLHPPGGGQLLTEQGKLGCSDLEEGGDAGADKKIPRKPPRFSCLLPSPQELIEIKSKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNSEVAQWLRENPRLDKKMIGEFVSDRKNLDLLESFVGTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRTCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNVPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNDEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLLVPAGSYDHDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSMRGPSTENQEAKRMALDCIKQCDPEKMITESKFLQLESLQELMKALISVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQALDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPPSLQATARTETTDTGAQSDSELSSYHPSDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADVVNSGWLVVGKDDIDSSKPGPGPNRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTIRIFVEASLNGGCKSQEKRSKCHKYDSKGNRFKKKSKDGSVLRRPRVTSQHTIRGGHSDDEDDEGVPASYHTVSLQLLDLMHTLHTRAATIYNSWAEEQRHLETGGKKIEADSRTLWAHCWCPLLQGIAWLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLPTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADTRGSGSSALWEITWERIDCFLPHLRDELFKQTVIPDPLPPVPTEPHSQKTLASAHLSPAASDTRMPSHTYTPEMPSELGAYDSEKSEVSGAAASGCSGSSTVLTPSKLSSSPEGPVSLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLAAPRPTDSMPTSEVN

Target ORF information:

RefSeq Version	XM_007479114.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007479114.2

ORF Insert Sequence:

ATGGGAGGCC TAGTGGAAGA GAATTCAGTG GTGGCTCACA GGTTTGCCAG GATGGTGGAT 
AAGAGTATTT ACATTATCCA AGGAGAGATT AACATTGTGG TAGGGGCCAT CAAACGGAAT 
GCAAGATGGA GTACTCATAC CCCTGTGGAT GAAGAACGAG ATCCTTTGCT GCATAGTTTC 
AGTCATCTAA AGGAAGTATT AAATAATATA ACAGATCTCT CCGAGATTGA GCCTAATATA 
TTCCTTCGAC CATTCCTGGA AGTTATTCGC TCTGAAGACA CCACTGGTCC CATCACTGGA 
CTGGCACTCA CCTCCGTCAA CAAATTTCTG TCATACTCCC TCATAGATCC CACTCATGAG 
GGCACAGCAG AAGGAATGGA GAACATGGCA GATGCCGTTA CCCATGCTCG TTTTGTGGGC 
ACAGACCCTG CCAGTGACGA GGTTGTCCTG ATGAAAATCC TTCAGGTTCT TCGGACTCTG 
CTGCTAACCC CAGTAGGTGC CCACCTGACC AACGAATCTG TGTGTGAAAT TATGCAGTCT 
TGCTTTCGGA TTTGCTTTGA GATGAGGCTC AGTGAGTTAT TGAGAAAATC CGCAGAGCAC 
ACTCTCGTCG ACATGGTGCA GCTGCTCTTC ACAAGGTTAC CTCAGTTTAA AGAAGAACCG 
AAGAACTACA TAGGGACAAA CATGAAGAAG CTAAAAATGA GAGCAGGAGG TATGAGTGAT 
TCATCCAAAT GGAAAAAGCA GAAGAGGTCT CCACGTCCAC CACGGCATGT CAGCAAAGTC 
ACAGAGTCAC CCACTCCCAA TGGAGCTACC CTGTCCTCCA ACTTTACAGG TGCTGTGTCC 
TTCATTGAAG CCTCCATGCC TATCTCTTCC ACAAGTTCTG AAGCCACCTC CACAGCTGTT 
AGTCCTTCAA CTGACAGTGG CCTTGACCTC TCCTCCCAGA CCACATCCAA GGAAGACCTC 
ACTGACCTGG AGCAGGCAGG CTCTCTAGGA CCCAACATAG CCATGGTGCC TGTAAACAAT 
GAATTGGGCT CTCCTGAATT GTCTGATCCC CAGCATGAAG TGTCCCAGGT GGAAAAGGTC 
CAGTCAGCAT CTGTGGAGTC CATCCCTGAG GTATTAGAAG AATGTAATTC TCTAGCCGAC 
CATTCAGATT CAGCCTCTGT CCATGACATG GACTATGTCA ACCCTCGGGG TGTTCGTTTT 
ACACAGTCCT CTCAGAAAGA AGGAACAGCA TTGGTCCCCT ATGGCCTCCC TTGTATTCGT 
GAGCTCTTCC GCTTCCTCAT CTCTCTTACC AACCCTCATG ACCGACACAA CTCAGAGGTC 
ATGATCCATA TGGGATTGCA GCTACTGACT GTGGCCCTTG AGTCTGCACC TGTGGCCCAG 
TGCCAGACTT TGTTAGGCCT CATCAAGGAT GAAATGTGCC GTCACCTATT CCAGCTGCTA 
AGTGTGGAGC GACTAAGCCT ATATGCAGCC TCTCTCCGAG TATGCTTCCT GCTCTTTGAG 
AGCATGCGAG AACACCTCAA GTTCCAAATG GAGATGTACA TCAAAAAACT CATGGAGATA 
ATCACTGTGG AGAACCCTAA GATGCCTTAT GAAATGAAGG AGATGGCACT AGAAGCTATA 
GTCCAGCTAT GGAGAATCCC CAGTTTTGTT ACTGAGCTCT ACATAAACTA TGACTGTGAC 
TACTATTGCT CCAACCTCTT TGAAGACCTC ACAAAGCTGC TGTCTAAGAA TGCCTTCCCT 
GTCTCTGGGC AGCTTTATAC AACACACCTG CTCTCTCTTG ATGCACTGTT GACAGTGATT 
GACAGTACGG AGGCCCATTG CCAGGCCAAA GTCTTGAACA ACCTCATCCA GCAGGAGAAG 
AAAGAGGCAG CTAAACCCAG TACTGAGATG ATGGATGGCA CGAAAGAAGT CAGCAGCAGC 
AGTGAACAAG CAGCCAGTGA TGGAAAACAC CCAGGGACAA ACCCCGATCA CCCAGGGCTG 
CATCCACCTG GGGGAGGGCA GTTGCTGACT GAGCAGGGAA AATTGGGCTG CAGTGACCTG 
GAGGAAGGTG GTGATGCAGG TGCTGACAAA AAAATTCCCC GGAAGCCACC TCGGTTTTCC 
TGCCTCCTTC CAAGTCCTCA GGAATTGATT GAGATTAAAA GCAAGAAAAA GCTACTGATC 
ACAGGCACAG AACAGTTCAA CCAGAAACCA AAGAAAGGGA TTCAGTTCTT ACAGGAGAAG 
GGTCTTCTGA CTATCCCCAT GGACAACAGT GAAGTTGCCC AGTGGCTTCG AGAGAACCCT 
AGGCTGGACA AGAAGATGAT TGGAGAGTTT GTGAGTGACC GAAAGAACCT TGATCTGCTG 
GAGAGCTTTG TGGGCACCTT CAGTTTCCAG GGTTTACGAC TAGATGAAGC TCTCCGTCTC 
TATTTGGAGG CCTTTCGCTT GCCAGGAGAA GCACCAGTCA TTCAGAGGTT ACTGGAGGCT 
TTTACAGAAC ACTGGAGGAC ATGTAATGGC TCCCCATTTG CAAATAGTGA TGCCTGCTTT 
GCCCTGGCCT ATGCTGTTAT CATGCTCAAC ACTGACCAGC ATAACCACAA TGTCCGAAAA 
CAGAATGTAC CCATGACCCT GGAGGAATTC CGAAAAAACC TGAAGGGCGT GAACGGAGGA 
AAAGATTTTG AGCAGGACAT CCTAGAGGAC ATGTACCATG CCATCAAGAA TGATGAGATT 
GTGATGCCTG AAGAACAGAC TGGTCTGGTG AGGGAGAACT ATGTATGGAA TGTCCTACTG 
CATCGAGGTG CAACCCCTGA GGGTATATTC CTACTGGTAC CTGCTGGCAG CTATGATCAT 
GACCTCTTCA CCATGACGTG GGGCCCTACT ATTGCTGCCC TCTCCTATGT ATTTGACAAG 
AGCCTAGAGG AAACAATTAT CCAAAAGGCC ATTTCAGGCT TCAGGAAATG TGCCATGATC 
TCCGCCCACT ATGGCCTGAG CGATGTGTTT GACAATCTCA TCATCTCTTT GTGCAAATTT 
ACAGCCCTCA GCAGTGAGTC CATTGAGAAC CTGCCCAGTG TGTTTGGGAG CAACCCTAAA 
GCTCACATTG CAGCCAAGAC TGTTTTCCAT TTGGCCCATC GCCATGGTGA CATCCTTCGA 
GAAGGTTGGA AGAACATCAT GGAAGCCATG CTACAGCTGT TCCGAGCCCA GCTATTGCCC 
AAGGCCATGG TAGAGGTGGA AGACTTTGTG GACCCCAATG GCAAGATCTC TCTGCAGCGG 
GAAGAAACAC CATCTAACCG AGGAGAGTCA ACTGTGTTGA GCTTTGTGAG CTGGTTGACG 
CTGAGTGGTC CTGAGCAATC CAGTATGCGG GGTCCATCCA CTGAGAACCA AGAAGCCAAG 
CGCATGGCCC TGGACTGTAT CAAGCAATGT GACCCAGAAA AGATGATCAC AGAAAGCAAG 
TTCCTCCAGC TGGAGTCATT ACAAGAGTTA ATGAAGGCTC TAATATCAGT GACACCAGAT 
GAAGAGACTT ATGATGAGGA AGATGCAGCA TTCTGCCTAG AGATGCTTCT TCGGATTGTG 
CTAGAGAACA GGGACCGTGT GGGCTGTGTA TGGCAGACAG TGCGTGATCA TCTGTACCAC 
CTCTGTGTCC AGGCGCTGGA TTTCTGCTTC CTGGTGGAAC GGGCTGTGGT GGGGTTACTT 
CGCCTGGCCA TACGGTTGCT AAGGAGAGAG GAAATCAGTG GCCAGGTGCT GCTGTCTCTT 
CGTATCCTGC TACTGATGAA ACCTAGTGTA CTGTCTCGTG TCAGCCACCA GGTTGCCTAT 
GGGCTCCATG AGTTACTCAA GACCAATGCA GCTAACATAC ACTCTGGGGA TGACTGGGCC 
ACACTTTTTA CTTTGCTGGA ATGCATTGGC TCAGGGGTGA AGCCTCCCCC TTCTTTGCAG 
GCCACAGCTA GGACAGAGAC CACTGATACT GGTGCTCAGT CAGACAGTGA ACTCTCTTCC 
TACCATCCCA GCGATGTCAG CTTGGATCGA GGCTACACCT CAGACTCAGA AGTCTATACT 
GATCACGGTA GACCAGGCAA GATGCACCGC TCAGCTACTG ATGCCGACGT GGTCAACAGT 
GGTTGGCTAG TGGTGGGGAA AGATGACATA GACAGCTCCA AGCCAGGACC AGGACCAAAC 
CGGCTTGGCC CTTCCCCTCT TGTGAATCAG TACAGCCTTA CTGTGGGCTT GGACTTGGGA 
CCTCATGACA CAAAGTCCCT CCTCAAATGT GTGGAGTCAC TGTCTTTCAT CGTTCGTGAT 
GCTGCTCATA TTACTCCAGA CAATTTTGAG CTCTGTGTCA AGACCATCCG AATTTTTGTA 
GAGGCCAGTC TGAATGGTGG ATGCAAATCT CAGGAAAAAC GAAGCAAATG CCACAAATAT 
GACAGCAAAG GGAACCGCTT CAAGAAGAAG TCGAAGGATG GCTCAGTTCT TCGTCGACCA 
CGAGTGACCA GCCAGCATAC AATTCGTGGC GGACACAGTG ATGATGAAGA TGATGAAGGA 
GTACCTGCTA GCTACCACAC GGTGTCTTTA CAGCTGCTGG ACTTGATGCA CACCCTACAC 
ACACGGGCAG CAACTATTTA CAACTCATGG GCTGAGGAGC AGCGTCACTT AGAGACAGGG 
GGCAAGAAGA TTGAGGCTGA TTCCCGGACC CTCTGGGCTC ATTGCTGGTG CCCCTTGCTT 
CAGGGTATTG CCTGGCTCTG CTGTGATGCC CGGCGTCAGG TACGGATGCA GGCACTCACC 
TACCTACAGA GGGCCCTGTT GGTACATGAC CTGCAGAAGC TAGATGCCCT GGAATGGGAG 
TCCTGTTTTA ATAAGGTGCT GTTTCCTCTG CTGACCAAGC TTCTAGAGAA CATCAGTCCT 
GCAGATGTGG GTGGGATGGA GGAGACACGG ATGAGGGCTT CCACACTGCT CTCCAAGGTT 
TTCCTGCAGC ACCTGTCCCC ACTGCTGTCA CTTCCAACCT TTGCTGCTCT CTGGCTGACC 
ATCCTGGACT TCATGGACAA GTATATGCAT GCAGGTTCCA GTGATTTACT GTCTGAGGCT 
ATCCCTGAGT CATTGAAGAA CATGCTGCTA GTGATGGATA CTGCAGAGAT CTTCCACAAC 
GCAGACACAA GAGGAAGTGG CTCCTCTGCC CTCTGGGAGA TCACCTGGGA GCGTATTGAC 
TGCTTCCTGC CCCATCTTCG AGATGAGCTC TTCAAGCAGA CAGTCATCCC TGATCCCCTG 
CCACCGGTGC CTACTGAACC ACATTCCCAG AAGACTCTGG CCTCTGCCCA TTTGTCTCCT 
GCTGCCAGTG ACACAAGGAT GCCCAGCCAC ACATATACTC CAGAAATGCC CTCAGAACTG 
GGGGCCTATG ACTCAGAGAA GTCAGAGGTC TCTGGAGCAG CTGCCAGTGG TTGTTCTGGT 
TCTTCCACAG TCTTGACTCC CAGCAAGCTG AGCTCCAGTC CAGAAGGGCC TGTGTCATTG 
GCCCAGCCCC CACTGATCCT GCAGCCTCTG GCTTCCCCAC TGCAAGTGGG TGTGCCACCC 
ATGACATTAC CTATCATTCT AAATCCAGCC CTCATTGAGG CAACTTCACC TGTGCCTCTG 
CTAGCTGCCC CTCGCCCCAC TGACTCTATG CCCACTTCTG AAGTCAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_007479115.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007479177.1 Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMc01221
Clone ID Related Accession (Same CDS sequence)	XM_007479115.2
Accession Version	XM_007479115.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007479115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGCC TAGTGGAAGA GAATTCAGTG GTGGCTCACA GGTTTGCCAG GATGGTGGAT 
AAGAGTATTT ACATTATCCA AGGAGAGATT AACATTGTGG TAGGGGCCAT CAAACGGAAT 
GCAAGATGGA GTACTCATAC CCCTGTGGAT GAAGAACGAG ATCCTTTGCT GCATAGTTTC 
AGTCATCTAA AGGAAGTATT AAATAATATA ACAGATCTCT CCGAGATTGA GCCTAATATA 
TTCCTTCGAC CATTCCTGGA AGTTATTCGC TCTGAAGACA CCACTGGTCC CATCACTGGA 
CTGGCACTCA CCTCCGTCAA CAAATTTCTG TCATACTCCC TCATAGATCC CACTCATGAG 
GGCACAGCAG AAGGAATGGA GAACATGGCA GATGCCGTTA CCCATGCTCG TTTTGTGGGC 
ACAGACCCTG CCAGTGACGA GGTTGTCCTG ATGAAAATCC TTCAGGTTCT TCGGACTCTG 
CTGCTAACCC CAGTAGGTGC CCACCTGACC AACGAATCTG TGTGTGAAAT TATGCAGTCT 
TGCTTTCGGA TTTGCTTTGA GATGAGGCTC AGTGAGTTAT TGAGAAAATC CGCAGAGCAC 
ACTCTCGTCG ACATGGTGCA GCTGCTCTTC ACAAGGTTAC CTCAGTTTAA AGAAGAACCG 
AAGAACTACA TAGGGACAAA CATGAAGAAG CTAAAAATGA GAGCAGGAGG TATGAGTGAT 
TCATCCAAAT GGAAAAAGCA GAAGAGGTCT CCACGTCCAC CACGGCATGT CAGCAAAGTC 
ACAGAGTCAC CCACTCCCAA TGGAGCTACC CTGTCCTCCA ACTTTACAGG TGCTGTGTCC 
TTCATTGAAG CCTCCATGCC TATCTCTTCC ACAAGTTCTG AAGCCACCTC CACAGCTGTT 
AGTCCTTCAA CTGACAGTGG CCTTGACCTC TCCTCCCAGA CCACATCCAA GGAAGACCTC 
ACTGACCTGG AGCAGGCAGG CTCTCTAGGA CCCAACATAG CCATGGTGCC TGTAAACAAT 
GAATTGGGCT CTCCTGAATT GTCTGATCCC CAGCATGAAG TGTCCCAGGT GGAAAAGGTC 
CAGTCAGCAT CTGTGGAGTC CATCCCTGAG GTATTAGAAG AATGTAATTC TCTAGCCGAC 
CATTCAGATT CAGCCTCTGT CCATGACATG GACTATGTCA ACCCTCGGGG TGTTCGTTTT 
ACACAGTCCT CTCAGAAAGA AGGAACAGCA TTGGTCCCCT ATGGCCTCCC TTGTATTCGT 
GAGCTCTTCC GCTTCCTCAT CTCTCTTACC AACCCTCATG ACCGACACAA CTCAGAGGTC 
ATGATCCATA TGGGATTGCA GCTACTGACT GTGGCCCTTG AGTCTGCACC TGTGGCCCAG 
TGCCAGACTT TGTTAGGCCT CATCAAGGAT GAAATGTGCC GTCACCTATT CCAGCTGCTA 
AGTGTGGAGC GACTAAGCCT ATATGCAGCC TCTCTCCGAG TATGCTTCCT GCTCTTTGAG 
AGCATGCGAG AACACCTCAA GTTCCAAATG GAGATGTACA TCAAAAAACT CATGGAGATA 
ATCACTGTGG AGAACCCTAA GATGCCTTAT GAAATGAAGG AGATGGCACT AGAAGCTATA 
GTCCAGCTAT GGAGAATCCC CAGTTTTGTT ACTGAGCTCT ACATAAACTA TGACTGTGAC 
TACTATTGCT CCAACCTCTT TGAAGACCTC ACAAAGCTGC TGTCTAAGAA TGCCTTCCCT 
GTCTCTGGGC AGCTTTATAC AACACACCTG CTCTCTCTTG ATGCACTGTT GACAGTGATT 
GACAGTACGG AGGCCCATTG CCAGGCCAAA GTCTTGAACA ACCTCATCCA GCAGGAGAAG 
AAAGAGGCAG CTAAACCCAG TACTGAGATG ATGGATGGCA CGAAAGAAGT CAGCAGCAGC 
AGTGAACAAG CAGCCAGTGA TGGAAAACAC CCAGGGACAA ACCCCGATCA CCCAGGGCTG 
CATCCACCTG GGGGAGGGCA GTTGCTGACT GAGCAGGGAA AATTGGGCTG CAGTGACCTG 
GAGGAAGCTG ACAAAAAAAT TCCCCGGAAG CCACCTCGGT TTTCCTGCCT CCTTCCAAGT 
CCTCAGGAAT TGATTGAGAT TAAAAGCAAG AAAAAGCTAC TGATCACAGG CACAGAACAG 
TTCAACCAGA AACCAAAGAA AGGGATTCAG TTCTTACAGG AGAAGGGTCT TCTGACTATC 
CCCATGGACA ACAGTGAAGT TGCCCAGTGG CTTCGAGAGA ACCCTAGGCT GGACAAGAAG 
ATGATTGGAG AGTTTGTGAG TGACCGAAAG AACCTTGATC TGCTGGAGAG CTTTGTGGGC 
ACCTTCAGTT TCCAGGGTTT ACGACTAGAT GAAGCTCTCC GTCTCTATTT GGAGGCCTTT 
CGCTTGCCAG GAGAAGCACC AGTCATTCAG AGGTTACTGG AGGCTTTTAC AGAACACTGG 
AGGACATGTA ATGGCTCCCC ATTTGCAAAT AGTGATGCCT GCTTTGCCCT GGCCTATGCT 
GTTATCATGC TCAACACTGA CCAGCATAAC CACAATGTCC GAAAACAGAA TGTACCCATG 
ACCCTGGAGG AATTCCGAAA AAACCTGAAG GGCGTGAACG GAGGAAAAGA TTTTGAGCAG 
GACATCCTAG AGGACATGTA CCATGCCATC AAGAATGATG AGATTGTGAT GCCTGAAGAA 
CAGACTGGTC TGGTGAGGGA GAACTATGTA TGGAATGTCC TACTGCATCG AGGTGCAACC 
CCTGAGGGTA TATTCCTACT GGTACCTGCT GGCAGCTATG ATCATGACCT CTTCACCATG 
ACGTGGGGCC CTACTATTGC TGCCCTCTCC TATGTATTTG ACAAGAGCCT AGAGGAAACA 
ATTATCCAAA AGGCCATTTC AGGCTTCAGG AAATGTGCCA TGATCTCCGC CCACTATGGC 
CTGAGCGATG TGTTTGACAA TCTCATCATC TCTTTGTGCA AATTTACAGC CCTCAGCAGT 
GAGTCCATTG AGAACCTGCC CAGTGTGTTT GGGAGCAACC CTAAAGCTCA CATTGCAGCC 
AAGACTGTTT TCCATTTGGC CCATCGCCAT GGTGACATCC TTCGAGAAGG TTGGAAGAAC 
ATCATGGAAG CCATGCTACA GCTGTTCCGA GCCCAGCTAT TGCCCAAGGC CATGGTAGAG 
GTGGAAGACT TTGTGGACCC CAATGGCAAG ATCTCTCTGC AGCGGGAAGA AACACCATCT 
AACCGAGGAG AGTCAACTGT GTTGAGCTTT GTGAGCTGGT TGACGCTGAG TGGTCCTGAG 
CAATCCAGTA TGCGGGGTCC ATCCACTGAG AACCAAGAAG CCAAGCGCAT GGCCCTGGAC 
TGTATCAAGC AATGTGACCC AGAAAAGATG ATCACAGAAA GCAAGTTCCT CCAGCTGGAG 
TCATTACAAG AGTTAATGAA GGCTCTAATA TCAGTGACAC CAGATGAAGA GACTTATGAT 
GAGGAAGATG CAGCATTCTG CCTAGAGATG CTTCTTCGGA TTGTGCTAGA GAACAGGGAC 
CGTGTGGGCT GTGTATGGCA GACAGTGCGT GATCATCTGT ACCACCTCTG TGTCCAGGCG 
CTGGATTTCT GCTTCCTGGT GGAACGGGCT GTGGTGGGGT TACTTCGCCT GGCCATACGG 
TTGCTAAGGA GAGAGGAAAT CAGTGGCCAG GTGCTGCTGT CTCTTCGTAT CCTGCTACTG 
ATGAAACCTA GTGTACTGTC TCGTGTCAGC CACCAGGTTG CCTATGGGCT CCATGAGTTA 
CTCAAGACCA ATGCAGCTAA CATACACTCT GGGGATGACT GGGCCACACT TTTTACTTTG 
CTGGAATGCA TTGGCTCAGG GGTGAAGCCT CCCCCTTCTT TGCAGGCCAC AGCTAGGACA 
GAGACCACTG ATACTGGTGC TCAGTCAGAC AGTGAACTCT CTTCCTACCA TCCCAGCGAT 
GTCAGCTTGG ATCGAGGCTA CACCTCAGAC TCAGAAGTCT ATACTGATCA CGGTAGACCA 
GGCAAGATGC ACCGCTCAGC TACTGATGCC GACGTGGTCA ACAGTGGTTG GCTAGTGGTG 
GGGAAAGATG ACATAGACAG CTCCAAGCCA GGACCAGGAC CAAACCGGCT TGGCCCTTCC 
CCTCTTGTGA ATCAGTACAG CCTTACTGTG GGCTTGGACT TGGGACCTCA TGACACAAAG 
TCCCTCCTCA AATGTGTGGA GTCACTGTCT TTCATCGTTC GTGATGCTGC TCATATTACT 
CCAGACAATT TTGAGCTCTG TGTCAAGACC ATCCGAATTT TTGTAGAGGC CAGTCTGAAT 
GGTGGATGCA AATCTCAGGA AAAACGAAGC AAATGCCACA AATATGACAG CAAAGGGAAC 
CGCTTCAAGA AGAAGTCGAA GGATGGCTCA GTTCTTCGTC GACCACGAGT GACCAGCCAG 
CATACAATTC GTGGCGGACA CAGTGATGAT GAAGATGATG AAGGAGTACC TGCTAGCTAC 
CACACGGTGT CTTTACAGGT CAGTCAGGAC CTGCTGGACT TGATGCACAC CCTACACACA 
CGGGCAGCAA CTATTTACAA CTCATGGGCT GAGGAGCAGC GTCACTTAGA GACAGGGGGC 
AAGAAGATTG AGGCTGATTC CCGGACCCTC TGGGCTCATT GCTGGTGCCC CTTGCTTCAG 
GGTATTGCCT GGCTCTGCTG TGATGCCCGG CGTCAGGTAC GGATGCAGGC ACTCACCTAC 
CTACAGAGGG CCCTGTTGGT ACATGACCTG CAGAAGCTAG ATGCCCTGGA ATGGGAGTCC 
TGTTTTAATA AGGTGCTGTT TCCTCTGCTG ACCAAGCTTC TAGAGAACAT CAGTCCTGCA 
GATGTGGGTG GGATGGAGGA GACACGGATG AGGGCTTCCA CACTGCTCTC CAAGGTTTTC 
CTGCAGCACC TGTCCCCACT GCTGTCACTT CCAACCTTTG CTGCTCTCTG GCTGACCATC 
CTGGACTTCA TGGACAAGTA TATGCATGCA GGTTCCAGTG ATTTACTGTC TGAGGCTATC 
CCTGAGTCAT TGAAGAACAT GCTGCTAGTG ATGGATACTG CAGAGATCTT CCACAACGCA 
GACACAAGAG GAAGTGGCTC CTCTGCCCTC TGGGAGATCA CCTGGGAGCG TATTGACTGC 
TTCCTGCCCC ATCTTCGAGA TGAGCTCTTC AAGCAGACAG TCATCCCTGA TCCCCTGCCA 
CCGGTGCCTA CTGAACCACA TTCCCAGAAG ACTCTGGCCT CTGCCCATTT GTCTCCTGCT 
GCCAGTGACA CAAGGATGCC CAGCCACACA TATACTCCAG AAATGCCCTC AGAACTGGGG 
GCCTATGACT CAGAGAAGTC AGAGGTCTCT GGAGCAGCTG CCAGTGGTTG TTCTGGTTCT 
TCCACAGTCT TGACTCCCAG CAAGCTGAGC TCCAGTCCAG AAGGGCCTGT GTCATTGGCC 
CAGCCCCCAC TGATCCTGCA GCCTCTGGCT TCCCCACTGC AAGTGGGTGT GCCACCCATG 
ACATTACCTA TCATTCTAAA TCCAGCCCTC ATTGAGGCAA CTTCACCTGT GCCTCTGCTA 
GCTGCCCCTC GCCCCACTGA CTCTATGCCC ACTTCTGAAG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007479177.1
CDS	69..5696
Translation	MGGLVEENSVVAHRFARMVDKSIYIIQGEINIVVGAIKRNARWSTHTPVDEERDPLLHSFSHLKEVLNNITDLSEIEPNIFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYSLIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYIGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHVSKVTESPTPNGATLSSNFTGAVSFIEASMPISSTSSEATSTAVSPSTDSGLDLSSQTTSKEDLTDLEQAGSLGPNIAMVPVNNELGSPELSDPQHEVSQVEKVQSASVESIPEVLEECNSLADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLQLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLSLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNNLIQQEKKEAAKPSTEMMDGTKEVSSSSEQAASDGKHPGTNPDHPGLHPPGGGQLLTEQGKLGCSDLEEADKKIPRKPPRFSCLLPSPQELIEIKSKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNSEVAQWLRENPRLDKKMIGEFVSDRKNLDLLESFVGTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRTCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNVPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNDEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLLVPAGSYDHDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSMRGPSTENQEAKRMALDCIKQCDPEKMITESKFLQLESLQELMKALISVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQALDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPPSLQATARTETTDTGAQSDSELSSYHPSDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADVVNSGWLVVGKDDIDSSKPGPGPNRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTIRIFVEASLNGGCKSQEKRSKCHKYDSKGNRFKKKSKDGSVLRRPRVTSQHTIRGGHSDDEDDEGVPASYHTVSLQVSQDLLDLMHTLHTRAATIYNSWAEEQRHLETGGKKIEADSRTLWAHCWCPLLQGIAWLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLPTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADTRGSGSSALWEITWERIDCFLPHLRDELFKQTVIPDPLPPVPTEPHSQKTLASAHLSPAASDTRMPSHTYTPEMPSELGAYDSEKSEVSGAAASGCSGSSTVLTPSKLSSSPEGPVSLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLAAPRPTDSMPTSEVN

Target ORF information:

RefSeq Version	XM_007479115.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007479115.2

ORF Insert Sequence:

ATGGGAGGCC TAGTGGAAGA GAATTCAGTG GTGGCTCACA GGTTTGCCAG GATGGTGGAT 
AAGAGTATTT ACATTATCCA AGGAGAGATT AACATTGTGG TAGGGGCCAT CAAACGGAAT 
GCAAGATGGA GTACTCATAC CCCTGTGGAT GAAGAACGAG ATCCTTTGCT GCATAGTTTC 
AGTCATCTAA AGGAAGTATT AAATAATATA ACAGATCTCT CCGAGATTGA GCCTAATATA 
TTCCTTCGAC CATTCCTGGA AGTTATTCGC TCTGAAGACA CCACTGGTCC CATCACTGGA 
CTGGCACTCA CCTCCGTCAA CAAATTTCTG TCATACTCCC TCATAGATCC CACTCATGAG 
GGCACAGCAG AAGGAATGGA GAACATGGCA GATGCCGTTA CCCATGCTCG TTTTGTGGGC 
ACAGACCCTG CCAGTGACGA GGTTGTCCTG ATGAAAATCC TTCAGGTTCT TCGGACTCTG 
CTGCTAACCC CAGTAGGTGC CCACCTGACC AACGAATCTG TGTGTGAAAT TATGCAGTCT 
TGCTTTCGGA TTTGCTTTGA GATGAGGCTC AGTGAGTTAT TGAGAAAATC CGCAGAGCAC 
ACTCTCGTCG ACATGGTGCA GCTGCTCTTC ACAAGGTTAC CTCAGTTTAA AGAAGAACCG 
AAGAACTACA TAGGGACAAA CATGAAGAAG CTAAAAATGA GAGCAGGAGG TATGAGTGAT 
TCATCCAAAT GGAAAAAGCA GAAGAGGTCT CCACGTCCAC CACGGCATGT CAGCAAAGTC 
ACAGAGTCAC CCACTCCCAA TGGAGCTACC CTGTCCTCCA ACTTTACAGG TGCTGTGTCC 
TTCATTGAAG CCTCCATGCC TATCTCTTCC ACAAGTTCTG AAGCCACCTC CACAGCTGTT 
AGTCCTTCAA CTGACAGTGG CCTTGACCTC TCCTCCCAGA CCACATCCAA GGAAGACCTC 
ACTGACCTGG AGCAGGCAGG CTCTCTAGGA CCCAACATAG CCATGGTGCC TGTAAACAAT 
GAATTGGGCT CTCCTGAATT GTCTGATCCC CAGCATGAAG TGTCCCAGGT GGAAAAGGTC 
CAGTCAGCAT CTGTGGAGTC CATCCCTGAG GTATTAGAAG AATGTAATTC TCTAGCCGAC 
CATTCAGATT CAGCCTCTGT CCATGACATG GACTATGTCA ACCCTCGGGG TGTTCGTTTT 
ACACAGTCCT CTCAGAAAGA AGGAACAGCA TTGGTCCCCT ATGGCCTCCC TTGTATTCGT 
GAGCTCTTCC GCTTCCTCAT CTCTCTTACC AACCCTCATG ACCGACACAA CTCAGAGGTC 
ATGATCCATA TGGGATTGCA GCTACTGACT GTGGCCCTTG AGTCTGCACC TGTGGCCCAG 
TGCCAGACTT TGTTAGGCCT CATCAAGGAT GAAATGTGCC GTCACCTATT CCAGCTGCTA 
AGTGTGGAGC GACTAAGCCT ATATGCAGCC TCTCTCCGAG TATGCTTCCT GCTCTTTGAG 
AGCATGCGAG AACACCTCAA GTTCCAAATG GAGATGTACA TCAAAAAACT CATGGAGATA 
ATCACTGTGG AGAACCCTAA GATGCCTTAT GAAATGAAGG AGATGGCACT AGAAGCTATA 
GTCCAGCTAT GGAGAATCCC CAGTTTTGTT ACTGAGCTCT ACATAAACTA TGACTGTGAC 
TACTATTGCT CCAACCTCTT TGAAGACCTC ACAAAGCTGC TGTCTAAGAA TGCCTTCCCT 
GTCTCTGGGC AGCTTTATAC AACACACCTG CTCTCTCTTG ATGCACTGTT GACAGTGATT 
GACAGTACGG AGGCCCATTG CCAGGCCAAA GTCTTGAACA ACCTCATCCA GCAGGAGAAG 
AAAGAGGCAG CTAAACCCAG TACTGAGATG ATGGATGGCA CGAAAGAAGT CAGCAGCAGC 
AGTGAACAAG CAGCCAGTGA TGGAAAACAC CCAGGGACAA ACCCCGATCA CCCAGGGCTG 
CATCCACCTG GGGGAGGGCA GTTGCTGACT GAGCAGGGAA AATTGGGCTG CAGTGACCTG 
GAGGAAGCTG ACAAAAAAAT TCCCCGGAAG CCACCTCGGT TTTCCTGCCT CCTTCCAAGT 
CCTCAGGAAT TGATTGAGAT TAAAAGCAAG AAAAAGCTAC TGATCACAGG CACAGAACAG 
TTCAACCAGA AACCAAAGAA AGGGATTCAG TTCTTACAGG AGAAGGGTCT TCTGACTATC 
CCCATGGACA ACAGTGAAGT TGCCCAGTGG CTTCGAGAGA ACCCTAGGCT GGACAAGAAG 
ATGATTGGAG AGTTTGTGAG TGACCGAAAG AACCTTGATC TGCTGGAGAG CTTTGTGGGC 
ACCTTCAGTT TCCAGGGTTT ACGACTAGAT GAAGCTCTCC GTCTCTATTT GGAGGCCTTT 
CGCTTGCCAG GAGAAGCACC AGTCATTCAG AGGTTACTGG AGGCTTTTAC AGAACACTGG 
AGGACATGTA ATGGCTCCCC ATTTGCAAAT AGTGATGCCT GCTTTGCCCT GGCCTATGCT 
GTTATCATGC TCAACACTGA CCAGCATAAC CACAATGTCC GAAAACAGAA TGTACCCATG 
ACCCTGGAGG AATTCCGAAA AAACCTGAAG GGCGTGAACG GAGGAAAAGA TTTTGAGCAG 
GACATCCTAG AGGACATGTA CCATGCCATC AAGAATGATG AGATTGTGAT GCCTGAAGAA 
CAGACTGGTC TGGTGAGGGA GAACTATGTA TGGAATGTCC TACTGCATCG AGGTGCAACC 
CCTGAGGGTA TATTCCTACT GGTACCTGCT GGCAGCTATG ATCATGACCT CTTCACCATG 
ACGTGGGGCC CTACTATTGC TGCCCTCTCC TATGTATTTG ACAAGAGCCT AGAGGAAACA 
ATTATCCAAA AGGCCATTTC AGGCTTCAGG AAATGTGCCA TGATCTCCGC CCACTATGGC 
CTGAGCGATG TGTTTGACAA TCTCATCATC TCTTTGTGCA AATTTACAGC CCTCAGCAGT 
GAGTCCATTG AGAACCTGCC CAGTGTGTTT GGGAGCAACC CTAAAGCTCA CATTGCAGCC 
AAGACTGTTT TCCATTTGGC CCATCGCCAT GGTGACATCC TTCGAGAAGG TTGGAAGAAC 
ATCATGGAAG CCATGCTACA GCTGTTCCGA GCCCAGCTAT TGCCCAAGGC CATGGTAGAG 
GTGGAAGACT TTGTGGACCC CAATGGCAAG ATCTCTCTGC AGCGGGAAGA AACACCATCT 
AACCGAGGAG AGTCAACTGT GTTGAGCTTT GTGAGCTGGT TGACGCTGAG TGGTCCTGAG 
CAATCCAGTA TGCGGGGTCC ATCCACTGAG AACCAAGAAG CCAAGCGCAT GGCCCTGGAC 
TGTATCAAGC AATGTGACCC AGAAAAGATG ATCACAGAAA GCAAGTTCCT CCAGCTGGAG 
TCATTACAAG AGTTAATGAA GGCTCTAATA TCAGTGACAC CAGATGAAGA GACTTATGAT 
GAGGAAGATG CAGCATTCTG CCTAGAGATG CTTCTTCGGA TTGTGCTAGA GAACAGGGAC 
CGTGTGGGCT GTGTATGGCA GACAGTGCGT GATCATCTGT ACCACCTCTG TGTCCAGGCG 
CTGGATTTCT GCTTCCTGGT GGAACGGGCT GTGGTGGGGT TACTTCGCCT GGCCATACGG 
TTGCTAAGGA GAGAGGAAAT CAGTGGCCAG GTGCTGCTGT CTCTTCGTAT CCTGCTACTG 
ATGAAACCTA GTGTACTGTC TCGTGTCAGC CACCAGGTTG CCTATGGGCT CCATGAGTTA 
CTCAAGACCA ATGCAGCTAA CATACACTCT GGGGATGACT GGGCCACACT TTTTACTTTG 
CTGGAATGCA TTGGCTCAGG GGTGAAGCCT CCCCCTTCTT TGCAGGCCAC AGCTAGGACA 
GAGACCACTG ATACTGGTGC TCAGTCAGAC AGTGAACTCT CTTCCTACCA TCCCAGCGAT 
GTCAGCTTGG ATCGAGGCTA CACCTCAGAC TCAGAAGTCT ATACTGATCA CGGTAGACCA 
GGCAAGATGC ACCGCTCAGC TACTGATGCC GACGTGGTCA ACAGTGGTTG GCTAGTGGTG 
GGGAAAGATG ACATAGACAG CTCCAAGCCA GGACCAGGAC CAAACCGGCT TGGCCCTTCC 
CCTCTTGTGA ATCAGTACAG CCTTACTGTG GGCTTGGACT TGGGACCTCA TGACACAAAG 
TCCCTCCTCA AATGTGTGGA GTCACTGTCT TTCATCGTTC GTGATGCTGC TCATATTACT 
CCAGACAATT TTGAGCTCTG TGTCAAGACC ATCCGAATTT TTGTAGAGGC CAGTCTGAAT 
GGTGGATGCA AATCTCAGGA AAAACGAAGC AAATGCCACA AATATGACAG CAAAGGGAAC 
CGCTTCAAGA AGAAGTCGAA GGATGGCTCA GTTCTTCGTC GACCACGAGT GACCAGCCAG 
CATACAATTC GTGGCGGACA CAGTGATGAT GAAGATGATG AAGGAGTACC TGCTAGCTAC 
CACACGGTGT CTTTACAGGT CAGTCAGGAC CTGCTGGACT TGATGCACAC CCTACACACA 
CGGGCAGCAA CTATTTACAA CTCATGGGCT GAGGAGCAGC GTCACTTAGA GACAGGGGGC 
AAGAAGATTG AGGCTGATTC CCGGACCCTC TGGGCTCATT GCTGGTGCCC CTTGCTTCAG 
GGTATTGCCT GGCTCTGCTG TGATGCCCGG CGTCAGGTAC GGATGCAGGC ACTCACCTAC 
CTACAGAGGG CCCTGTTGGT ACATGACCTG CAGAAGCTAG ATGCCCTGGA ATGGGAGTCC 
TGTTTTAATA AGGTGCTGTT TCCTCTGCTG ACCAAGCTTC TAGAGAACAT CAGTCCTGCA 
GATGTGGGTG GGATGGAGGA GACACGGATG AGGGCTTCCA CACTGCTCTC CAAGGTTTTC 
CTGCAGCACC TGTCCCCACT GCTGTCACTT CCAACCTTTG CTGCTCTCTG GCTGACCATC 
CTGGACTTCA TGGACAAGTA TATGCATGCA GGTTCCAGTG ATTTACTGTC TGAGGCTATC 
CCTGAGTCAT TGAAGAACAT GCTGCTAGTG ATGGATACTG CAGAGATCTT CCACAACGCA 
GACACAAGAG GAAGTGGCTC CTCTGCCCTC TGGGAGATCA CCTGGGAGCG TATTGACTGC 
TTCCTGCCCC ATCTTCGAGA TGAGCTCTTC AAGCAGACAG TCATCCCTGA TCCCCTGCCA 
CCGGTGCCTA CTGAACCACA TTCCCAGAAG ACTCTGGCCT CTGCCCATTT GTCTCCTGCT 
GCCAGTGACA CAAGGATGCC CAGCCACACA TATACTCCAG AAATGCCCTC AGAACTGGGG 
GCCTATGACT CAGAGAAGTC AGAGGTCTCT GGAGCAGCTG CCAGTGGTTG TTCTGGTTCT 
TCCACAGTCT TGACTCCCAG CAAGCTGAGC TCCAGTCCAG AAGGGCCTGT GTCATTGGCC 
CAGCCCCCAC TGATCCTGCA GCCTCTGGCT TCCCCACTGC AAGTGGGTGT GCCACCCATG 
ACATTACCTA TCATTCTAAA TCCAGCCCTC ATTGAGGCAA CTTCACCTGT GCCTCTGCTA 
GCTGCCCCTC GCCCCACTGA CTCTATGCCC ACTTCTGAAG TCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GBF1 ( XM_007479116.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007479178.1 Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

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CloneID	OMc01222
Clone ID Related Accession (Same CDS sequence)	XM_007479116.2
Accession Version	XM_007479116.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007479116.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATA AGAGTATTTA CATTATCCAA GGAGAGATTA ACATTGTGGT AGGGGCCATC 
AAACGGAATG CAAGATGGAG TACTCATACC CCTGTGGATG AAGAACGAGA TCCTTTGCTG 
CATAGTTTCA GTCATCTAAA GGAAGTATTA AATAATATAA CAGATCTCTC CGAGATTGAG 
CCTAATATAT TCCTTCGACC ATTCCTGGAA GTTATTCGCT CTGAAGACAC CACTGGTCCC 
ATCACTGGAC TGGCACTCAC CTCCGTCAAC AAATTTCTGT CATACTCCCT CATAGATCCC 
ACTCATGAGG GCACAGCAGA AGGAATGGAG AACATGGCAG ATGCCGTTAC CCATGCTCGT 
TTTGTGGGCA CAGACCCTGC CAGTGACGAG GTTGTCCTGA TGAAAATCCT TCAGGTTCTT 
CGGACTCTGC TGCTAACCCC AGTAGGTGCC CACCTGACCA ACGAATCTGT GTGTGAAATT 
ATGCAGTCTT GCTTTCGGAT TTGCTTTGAG ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCGA AGAACTACAT AGGGACAAAC ATGAAGAAGC TAAAAATGAG AGCAGGAGGT 
ATGAGTGATT CATCCAAATG GAAAAAGCAG AAGAGGTCTC CACGTCCACC ACGGCATGTC 
AGCAAAGTCA CAGAGTCACC CACTCCCAAT GGAGCTACCC TGTCCTCCAA CTTTACAGGT 
GCTGTGTCCT TCATTGAAGC CTCCATGCCT ATCTCTTCCA CAAGTTCTGA AGCCACCTCC 
ACAGCTGTTA GTCCTTCAAC TGACAGTGGC CTTGACCTCT CCTCCCAGAC CACATCCAAG 
GAAGACCTCA CTGACCTGGA GCAGGCAGGC TCTCTAGGAC CCAACATAGC CATGGTGCCT 
GTAAACAATG AATTGGGCTC TCCTGAATTG TCTGATCCCC AGCATGAAGT GTCCCAGGTG 
GAAAAGGTCC AGTCAGCATC TGTGGAGTCC ATCCCTGAGG TATTAGAAGA ATGTAATTCT 
CTAGCCGACC ATTCAGATTC AGCCTCTGTC CATGACATGG ACTATGTCAA CCCTCGGGGT 
GTTCGTTTTA CACAGTCCTC TCAGAAAGAA GGAACAGCAT TGGTCCCCTA TGGCCTCCCT 
TGTATTCGTG AGCTCTTCCG CTTCCTCATC TCTCTTACCA ACCCTCATGA CCGACACAAC 
TCAGAGGTCA TGATCCATAT GGGATTGCAG CTACTGACTG TGGCCCTTGA GTCTGCACCT 
GTGGCCCAGT GCCAGACTTT GTTAGGCCTC ATCAAGGATG AAATGTGCCG TCACCTATTC 
CAGCTGCTAA GTGTGGAGCG ACTAAGCCTA TATGCAGCCT CTCTCCGAGT ATGCTTCCTG 
CTCTTTGAGA GCATGCGAGA ACACCTCAAG TTCCAAATGG AGATGTACAT CAAAAAACTC 
ATGGAGATAA TCACTGTGGA GAACCCTAAG ATGCCTTATG AAATGAAGGA GATGGCACTA 
GAAGCTATAG TCCAGCTATG GAGAATCCCC AGTTTTGTTA CTGAGCTCTA CATAAACTAT 
GACTGTGACT ACTATTGCTC CAACCTCTTT GAAGACCTCA CAAAGCTGCT GTCTAAGAAT 
GCCTTCCCTG TCTCTGGGCA GCTTTATACA ACACACCTGC TCTCTCTTGA TGCACTGTTG 
ACAGTGATTG ACAGTACGGA GGCCCATTGC CAGGCCAAAG TCTTGAACAA CCTCATCCAG 
CAGGAGAAGA AAGAGGCAGC TAAACCCAGT ACTGAGATGA TGGATGGCAC GAAAGAAGTC 
AGCAGCAGCA GTGAACAAGC AGCCAGTGAT GGAAAACACC CAGGGACAAA CCCCGATCAC 
CCAGGGCTGC ATCCACCTGG GGGAGGGCAG TTGCTGACTG AGCAGGGAAA ATTGGGCTGC 
AGTGACCTGG AGGAAGGTGG TGATGCAGGT GCTGACAAAA AAATTCCCCG GAAGCCACCT 
CGGTTTTCCT GCCTCCTTCC AAGTCCTCAG GAATTGATTG AGATTAAAAG CAAGAAAAAG 
CTACTGATCA CAGGCACAGA ACAGTTCAAC CAGAAACCAA AGAAAGGGAT TCAGTTCTTA 
CAGGAGAAGG GTCTTCTGAC TATCCCCATG GACAACAGTG AAGTTGCCCA GTGGCTTCGA 
GAGAACCCTA GGCTGGACAA GAAGATGATT GGAGAGTTTG TGAGTGACCG AAAGAACCTT 
GATCTGCTGG AGAGCTTTGT GGGCACCTTC AGTTTCCAGG GTTTACGACT AGATGAAGCT 
CTCCGTCTCT ATTTGGAGGC CTTTCGCTTG CCAGGAGAAG CACCAGTCAT TCAGAGGTTA 
CTGGAGGCTT TTACAGAACA CTGGAGGACA TGTAATGGCT CCCCATTTGC AAATAGTGAT 
GCCTGCTTTG CCCTGGCCTA TGCTGTTATC ATGCTCAACA CTGACCAGCA TAACCACAAT 
GTCCGAAAAC AGAATGTACC CATGACCCTG GAGGAATTCC GAAAAAACCT GAAGGGCGTG 
AACGGAGGAA AAGATTTTGA GCAGGACATC CTAGAGGACA TGTACCATGC CATCAAGAAT 
GATGAGATTG TGATGCCTGA AGAACAGACT GGTCTGGTGA GGGAGAACTA TGTATGGAAT 
GTCCTACTGC ATCGAGGTGC AACCCCTGAG GGTATATTCC TACTGGTACC TGCTGGCAGC 
TATGATCATG ACCTCTTCAC CATGACGTGG GGCCCTACTA TTGCTGCCCT CTCCTATGTA 
TTTGACAAGA GCCTAGAGGA AACAATTATC CAAAAGGCCA TTTCAGGCTT CAGGAAATGT 
GCCATGATCT CCGCCCACTA TGGCCTGAGC GATGTGTTTG ACAATCTCAT CATCTCTTTG 
TGCAAATTTA CAGCCCTCAG CAGTGAGTCC ATTGAGAACC TGCCCAGTGT GTTTGGGAGC 
AACCCTAAAG CTCACATTGC AGCCAAGACT GTTTTCCATT TGGCCCATCG CCATGGTGAC 
ATCCTTCGAG AAGGTTGGAA GAACATCATG GAAGCCATGC TACAGCTGTT CCGAGCCCAG 
CTATTGCCCA AGGCCATGGT AGAGGTGGAA GACTTTGTGG ACCCCAATGG CAAGATCTCT 
CTGCAGCGGG AAGAAACACC ATCTAACCGA GGAGAGTCAA CTGTGTTGAG CTTTGTGAGC 
TGGTTGACGC TGAGTGGTCC TGAGCAATCC AGTATGCGGG GTCCATCCAC TGAGAACCAA 
GAAGCCAAGC GCATGGCCCT GGACTGTATC AAGCAATGTG ACCCAGAAAA GATGATCACA 
GAAAGCAAGT TCCTCCAGCT GGAGTCATTA CAAGAGTTAA TGAAGGCTCT AATATCAGTG 
ACACCAGATG AAGAGACTTA TGATGAGGAA GATGCAGCAT TCTGCCTAGA GATGCTTCTT 
CGGATTGTGC TAGAGAACAG GGACCGTGTG GGCTGTGTAT GGCAGACAGT GCGTGATCAT 
CTGTACCACC TCTGTGTCCA GGCGCTGGAT TTCTGCTTCC TGGTGGAACG GGCTGTGGTG 
GGGTTACTTC GCCTGGCCAT ACGGTTGCTA AGGAGAGAGG AAATCAGTGG CCAGGTGCTG 
CTGTCTCTTC GTATCCTGCT ACTGATGAAA CCTAGTGTAC TGTCTCGTGT CAGCCACCAG 
GTTGCCTATG GGCTCCATGA GTTACTCAAG ACCAATGCAG CTAACATACA CTCTGGGGAT 
GACTGGGCCA CACTTTTTAC TTTGCTGGAA TGCATTGGCT CAGGGGTGAA GCCTCCCCCT 
TCTTTGCAGG CCACAGCTAG GACAGAGACC ACTGATACTG GTGCTCAGTC AGACAGTGAA 
CTCTCTTCCT ACCATCCCAG CGATGTCAGC TTGGATCGAG GCTACACCTC AGACTCAGAA 
GTCTATACTG ATCACGGTAG ACCAGGCAAG ATGCACCGCT CAGCTACTGA TGCCGACGTG 
GTCAACAGTG GTTGGCTAGT GGTGGGGAAA GATGACATAG ACAGCTCCAA GCCAGGACCA 
GGACCAAACC GGCTTGGCCC TTCCCCTCTT GTGAATCAGT ACAGCCTTAC TGTGGGCTTG 
GACTTGGGAC CTCATGACAC AAAGTCCCTC CTCAAATGTG TGGAGTCACT GTCTTTCATC 
GTTCGTGATG CTGCTCATAT TACTCCAGAC AATTTTGAGC TCTGTGTCAA GACCATCCGA 
ATTTTTGTAG AGGCCAGTCT GAATGGTGGA TGCAAATCTC AGGAAAAACG AAGCAAATGC 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAGT CGAAGGATGG CTCAGTTCTT 
CGTCGACCAC GAGTGACCAG CCAGCATACA ATTCGTGGCG GACACAGTGA TGATGAAGAT 
GATGAAGGAG TACCTGCTAG CTACCACACG GTGTCTTTAC AGGTCAGTCA GGACCTGCTG 
GACTTGATGC ACACCCTACA CACACGGGCA GCAACTATTT ACAACTCATG GGCTGAGGAG 
CAGCGTCACT TAGAGACAGG GGGCAAGAAG ATTGAGGCTG ATTCCCGGAC CCTCTGGGCT 
CATTGCTGGT GCCCCTTGCT TCAGGGTATT GCCTGGCTCT GCTGTGATGC CCGGCGTCAG 
GTACGGATGC AGGCACTCAC CTACCTACAG AGGGCCCTGT TGGTACATGA CCTGCAGAAG 
CTAGATGCCC TGGAATGGGA GTCCTGTTTT AATAAGGTGC TGTTTCCTCT GCTGACCAAG 
CTTCTAGAGA ACATCAGTCC TGCAGATGTG GGTGGGATGG AGGAGACACG GATGAGGGCT 
TCCACACTGC TCTCCAAGGT TTTCCTGCAG CACCTGTCCC CACTGCTGTC ACTTCCAACC 
TTTGCTGCTC TCTGGCTGAC CATCCTGGAC TTCATGGACA AGTATATGCA TGCAGGTTCC 
AGTGATTTAC TGTCTGAGGC TATCCCTGAG TCATTGAAGA ACATGCTGCT AGTGATGGAT 
ACTGCAGAGA TCTTCCACAA CGCAGACACA AGAGGAAGTG GCTCCTCTGC CCTCTGGGAG 
ATCACCTGGG AGCGTATTGA CTGCTTCCTG CCCCATCTTC GAGATGAGCT CTTCAAGCAG 
ACAGTCATCC CTGATCCCCT GCCACCGGTG CCTACTGAAC CACATTCCCA GAAGACTCTG 
GCCTCTGCCC ATTTGTCTCC TGCTGCCAGT GACACAAGGA TGCCCAGCCA CACATATACT 
CCAGAAATGC CCTCAGAACT GGGGGCCTAT GACTCAGAGA AGTCAGAGGT CTCTGGAGCA 
GCTGCCAGTG GTTGTTCTGG TTCTTCCACA GTCTTGACTC CCAGCAAGCT GAGCTCCAGT 
CCAGAAGGGC CTGTGTCATT GGCCCAGCCC CCACTGATCC TGCAGCCTCT GGCTTCCCCA 
CTGCAAGTGG GTGTGCCACC CATGACATTA CCTATCATTC TAAATCCAGC CCTCATTGAG 
GCAACTTCAC CTGTGCCTCT GCTAGCTGCC CCTCGCCCCA CTGACTCTAT GCCCACTTCT 
GAAGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007479178.1
CDS	298..5889
Translation	MVDKSIYIIQGEINIVVGAIKRNARWSTHTPVDEERDPLLHSFSHLKEVLNNITDLSEIEPNIFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYSLIDPTHEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYIGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHVSKVTESPTPNGATLSSNFTGAVSFIEASMPISSTSSEATSTAVSPSTDSGLDLSSQTTSKEDLTDLEQAGSLGPNIAMVPVNNELGSPELSDPQHEVSQVEKVQSASVESIPEVLEECNSLADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDRHNSEVMIHMGLQLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSVERLSLYAASLRVCFLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYINYDCDYYCSNLFEDLTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNNLIQQEKKEAAKPSTEMMDGTKEVSSSSEQAASDGKHPGTNPDHPGLHPPGGGQLLTEQGKLGCSDLEEGGDAGADKKIPRKPPRFSCLLPSPQELIEIKSKKKLLITGTEQFNQKPKKGIQFLQEKGLLTIPMDNSEVAQWLRENPRLDKKMIGEFVSDRKNLDLLESFVGTFSFQGLRLDEALRLYLEAFRLPGEAPVIQRLLEAFTEHWRTCNGSPFANSDACFALAYAVIMLNTDQHNHNVRKQNVPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNDEIVMPEEQTGLVRENYVWNVLLHRGATPEGIFLLVPAGSYDHDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLPKAMVEVEDFVDPNGKISLQREETPSNRGESTVLSFVSWLTLSGPEQSSMRGPSTENQEAKRMALDCIKQCDPEKMITESKFLQLESLQELMKALISVTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQALDFCFLVERAVVGLLRLAIRLLRREEISGQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSGDDWATLFTLLECIGSGVKPPPSLQATARTETTDTGAQSDSELSSYHPSDVSLDRGYTSDSEVYTDHGRPGKMHRSATDADVVNSGWLVVGKDDIDSSKPGPGPNRLGPSPLVNQYSLTVGLDLGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTIRIFVEASLNGGCKSQEKRSKCHKYDSKGNRFKKKSKDGSVLRRPRVTSQHTIRGGHSDDEDDEGVPASYHTVSLQVSQDLLDLMHTLHTRAATIYNSWAEEQRHLETGGKKIEADSRTLWAHCWCPLLQGIAWLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLPTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHNADTRGSGSSALWEITWERIDCFLPHLRDELFKQTVIPDPLPPVPTEPHSQKTLASAHLSPAASDTRMPSHTYTPEMPSELGAYDSEKSEVSGAAASGCSGSSTVLTPSKLSSSPEGPVSLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLAAPRPTDSMPTSEVN

Target ORF information:

RefSeq Version	XM_007479116.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica golgi brefeldin A resistant guanine nucleotide exchange factor 1 (GBF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007479116.2

ORF Insert Sequence:

ATGGTGGATA AGAGTATTTA CATTATCCAA GGAGAGATTA ACATTGTGGT AGGGGCCATC 
AAACGGAATG CAAGATGGAG TACTCATACC CCTGTGGATG AAGAACGAGA TCCTTTGCTG 
CATAGTTTCA GTCATCTAAA GGAAGTATTA AATAATATAA CAGATCTCTC CGAGATTGAG 
CCTAATATAT TCCTTCGACC ATTCCTGGAA GTTATTCGCT CTGAAGACAC CACTGGTCCC 
ATCACTGGAC TGGCACTCAC CTCCGTCAAC AAATTTCTGT CATACTCCCT CATAGATCCC 
ACTCATGAGG GCACAGCAGA AGGAATGGAG AACATGGCAG ATGCCGTTAC CCATGCTCGT 
TTTGTGGGCA CAGACCCTGC CAGTGACGAG GTTGTCCTGA TGAAAATCCT TCAGGTTCTT 
CGGACTCTGC TGCTAACCCC AGTAGGTGCC CACCTGACCA ACGAATCTGT GTGTGAAATT 
ATGCAGTCTT GCTTTCGGAT TTGCTTTGAG ATGAGGCTCA GTGAGTTATT GAGAAAATCC 
GCAGAGCACA CTCTCGTCGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA 
GAAGAACCGA AGAACTACAT AGGGACAAAC ATGAAGAAGC TAAAAATGAG AGCAGGAGGT 
ATGAGTGATT CATCCAAATG GAAAAAGCAG AAGAGGTCTC CACGTCCACC ACGGCATGTC 
AGCAAAGTCA CAGAGTCACC CACTCCCAAT GGAGCTACCC TGTCCTCCAA CTTTACAGGT 
GCTGTGTCCT TCATTGAAGC CTCCATGCCT ATCTCTTCCA CAAGTTCTGA AGCCACCTCC 
ACAGCTGTTA GTCCTTCAAC TGACAGTGGC CTTGACCTCT CCTCCCAGAC CACATCCAAG 
GAAGACCTCA CTGACCTGGA GCAGGCAGGC TCTCTAGGAC CCAACATAGC CATGGTGCCT 
GTAAACAATG AATTGGGCTC TCCTGAATTG TCTGATCCCC AGCATGAAGT GTCCCAGGTG 
GAAAAGGTCC AGTCAGCATC TGTGGAGTCC ATCCCTGAGG TATTAGAAGA ATGTAATTCT 
CTAGCCGACC ATTCAGATTC AGCCTCTGTC CATGACATGG ACTATGTCAA CCCTCGGGGT 
GTTCGTTTTA CACAGTCCTC TCAGAAAGAA GGAACAGCAT TGGTCCCCTA TGGCCTCCCT 
TGTATTCGTG AGCTCTTCCG CTTCCTCATC TCTCTTACCA ACCCTCATGA CCGACACAAC 
TCAGAGGTCA TGATCCATAT GGGATTGCAG CTACTGACTG TGGCCCTTGA GTCTGCACCT 
GTGGCCCAGT GCCAGACTTT GTTAGGCCTC ATCAAGGATG AAATGTGCCG TCACCTATTC 
CAGCTGCTAA GTGTGGAGCG ACTAAGCCTA TATGCAGCCT CTCTCCGAGT ATGCTTCCTG 
CTCTTTGAGA GCATGCGAGA ACACCTCAAG TTCCAAATGG AGATGTACAT CAAAAAACTC 
ATGGAGATAA TCACTGTGGA GAACCCTAAG ATGCCTTATG AAATGAAGGA GATGGCACTA 
GAAGCTATAG TCCAGCTATG GAGAATCCCC AGTTTTGTTA CTGAGCTCTA CATAAACTAT 
GACTGTGACT ACTATTGCTC CAACCTCTTT GAAGACCTCA CAAAGCTGCT GTCTAAGAAT 
GCCTTCCCTG TCTCTGGGCA GCTTTATACA ACACACCTGC TCTCTCTTGA TGCACTGTTG 
ACAGTGATTG ACAGTACGGA GGCCCATTGC CAGGCCAAAG TCTTGAACAA CCTCATCCAG 
CAGGAGAAGA AAGAGGCAGC TAAACCCAGT ACTGAGATGA TGGATGGCAC GAAAGAAGTC 
AGCAGCAGCA GTGAACAAGC AGCCAGTGAT GGAAAACACC CAGGGACAAA CCCCGATCAC 
CCAGGGCTGC ATCCACCTGG GGGAGGGCAG TTGCTGACTG AGCAGGGAAA ATTGGGCTGC 
AGTGACCTGG AGGAAGGTGG TGATGCAGGT GCTGACAAAA AAATTCCCCG GAAGCCACCT 
CGGTTTTCCT GCCTCCTTCC AAGTCCTCAG GAATTGATTG AGATTAAAAG CAAGAAAAAG 
CTACTGATCA CAGGCACAGA ACAGTTCAAC CAGAAACCAA AGAAAGGGAT TCAGTTCTTA 
CAGGAGAAGG GTCTTCTGAC TATCCCCATG GACAACAGTG AAGTTGCCCA GTGGCTTCGA 
GAGAACCCTA GGCTGGACAA GAAGATGATT GGAGAGTTTG TGAGTGACCG AAAGAACCTT 
GATCTGCTGG AGAGCTTTGT GGGCACCTTC AGTTTCCAGG GTTTACGACT AGATGAAGCT 
CTCCGTCTCT ATTTGGAGGC CTTTCGCTTG CCAGGAGAAG CACCAGTCAT TCAGAGGTTA 
CTGGAGGCTT TTACAGAACA CTGGAGGACA TGTAATGGCT CCCCATTTGC AAATAGTGAT 
GCCTGCTTTG CCCTGGCCTA TGCTGTTATC ATGCTCAACA CTGACCAGCA TAACCACAAT 
GTCCGAAAAC AGAATGTACC CATGACCCTG GAGGAATTCC GAAAAAACCT GAAGGGCGTG 
AACGGAGGAA AAGATTTTGA GCAGGACATC CTAGAGGACA TGTACCATGC CATCAAGAAT 
GATGAGATTG TGATGCCTGA AGAACAGACT GGTCTGGTGA GGGAGAACTA TGTATGGAAT 
GTCCTACTGC ATCGAGGTGC AACCCCTGAG GGTATATTCC TACTGGTACC TGCTGGCAGC 
TATGATCATG ACCTCTTCAC CATGACGTGG GGCCCTACTA TTGCTGCCCT CTCCTATGTA 
TTTGACAAGA GCCTAGAGGA AACAATTATC CAAAAGGCCA TTTCAGGCTT CAGGAAATGT 
GCCATGATCT CCGCCCACTA TGGCCTGAGC GATGTGTTTG ACAATCTCAT CATCTCTTTG 
TGCAAATTTA CAGCCCTCAG CAGTGAGTCC ATTGAGAACC TGCCCAGTGT GTTTGGGAGC 
AACCCTAAAG CTCACATTGC AGCCAAGACT GTTTTCCATT TGGCCCATCG CCATGGTGAC 
ATCCTTCGAG AAGGTTGGAA GAACATCATG GAAGCCATGC TACAGCTGTT CCGAGCCCAG 
CTATTGCCCA AGGCCATGGT AGAGGTGGAA GACTTTGTGG ACCCCAATGG CAAGATCTCT 
CTGCAGCGGG AAGAAACACC ATCTAACCGA GGAGAGTCAA CTGTGTTGAG CTTTGTGAGC 
TGGTTGACGC TGAGTGGTCC TGAGCAATCC AGTATGCGGG GTCCATCCAC TGAGAACCAA 
GAAGCCAAGC GCATGGCCCT GGACTGTATC AAGCAATGTG ACCCAGAAAA GATGATCACA 
GAAAGCAAGT TCCTCCAGCT GGAGTCATTA CAAGAGTTAA TGAAGGCTCT AATATCAGTG 
ACACCAGATG AAGAGACTTA TGATGAGGAA GATGCAGCAT TCTGCCTAGA GATGCTTCTT 
CGGATTGTGC TAGAGAACAG GGACCGTGTG GGCTGTGTAT GGCAGACAGT GCGTGATCAT 
CTGTACCACC TCTGTGTCCA GGCGCTGGAT TTCTGCTTCC TGGTGGAACG GGCTGTGGTG 
GGGTTACTTC GCCTGGCCAT ACGGTTGCTA AGGAGAGAGG AAATCAGTGG CCAGGTGCTG 
CTGTCTCTTC GTATCCTGCT ACTGATGAAA CCTAGTGTAC TGTCTCGTGT CAGCCACCAG 
GTTGCCTATG GGCTCCATGA GTTACTCAAG ACCAATGCAG CTAACATACA CTCTGGGGAT 
GACTGGGCCA CACTTTTTAC TTTGCTGGAA TGCATTGGCT CAGGGGTGAA GCCTCCCCCT 
TCTTTGCAGG CCACAGCTAG GACAGAGACC ACTGATACTG GTGCTCAGTC AGACAGTGAA 
CTCTCTTCCT ACCATCCCAG CGATGTCAGC TTGGATCGAG GCTACACCTC AGACTCAGAA 
GTCTATACTG ATCACGGTAG ACCAGGCAAG ATGCACCGCT CAGCTACTGA TGCCGACGTG 
GTCAACAGTG GTTGGCTAGT GGTGGGGAAA GATGACATAG ACAGCTCCAA GCCAGGACCA 
GGACCAAACC GGCTTGGCCC TTCCCCTCTT GTGAATCAGT ACAGCCTTAC TGTGGGCTTG 
GACTTGGGAC CTCATGACAC AAAGTCCCTC CTCAAATGTG TGGAGTCACT GTCTTTCATC 
GTTCGTGATG CTGCTCATAT TACTCCAGAC AATTTTGAGC TCTGTGTCAA GACCATCCGA 
ATTTTTGTAG AGGCCAGTCT GAATGGTGGA TGCAAATCTC AGGAAAAACG AAGCAAATGC 
CACAAATATG ACAGCAAAGG GAACCGCTTC AAGAAGAAGT CGAAGGATGG CTCAGTTCTT 
CGTCGACCAC GAGTGACCAG CCAGCATACA ATTCGTGGCG GACACAGTGA TGATGAAGAT 
GATGAAGGAG TACCTGCTAG CTACCACACG GTGTCTTTAC AGGTCAGTCA GGACCTGCTG 
GACTTGATGC ACACCCTACA CACACGGGCA GCAACTATTT ACAACTCATG GGCTGAGGAG 
CAGCGTCACT TAGAGACAGG GGGCAAGAAG ATTGAGGCTG ATTCCCGGAC CCTCTGGGCT 
CATTGCTGGT GCCCCTTGCT TCAGGGTATT GCCTGGCTCT GCTGTGATGC CCGGCGTCAG 
GTACGGATGC AGGCACTCAC CTACCTACAG AGGGCCCTGT TGGTACATGA CCTGCAGAAG 
CTAGATGCCC TGGAATGGGA GTCCTGTTTT AATAAGGTGC TGTTTCCTCT GCTGACCAAG 
CTTCTAGAGA ACATCAGTCC TGCAGATGTG GGTGGGATGG AGGAGACACG GATGAGGGCT 
TCCACACTGC TCTCCAAGGT TTTCCTGCAG CACCTGTCCC CACTGCTGTC ACTTCCAACC 
TTTGCTGCTC TCTGGCTGAC CATCCTGGAC TTCATGGACA AGTATATGCA TGCAGGTTCC 
AGTGATTTAC TGTCTGAGGC TATCCCTGAG TCATTGAAGA ACATGCTGCT AGTGATGGAT 
ACTGCAGAGA TCTTCCACAA CGCAGACACA AGAGGAAGTG GCTCCTCTGC CCTCTGGGAG 
ATCACCTGGG AGCGTATTGA CTGCTTCCTG CCCCATCTTC GAGATGAGCT CTTCAAGCAG 
ACAGTCATCC CTGATCCCCT GCCACCGGTG CCTACTGAAC CACATTCCCA GAAGACTCTG 
GCCTCTGCCC ATTTGTCTCC TGCTGCCAGT GACACAAGGA TGCCCAGCCA CACATATACT 
CCAGAAATGC CCTCAGAACT GGGGGCCTAT GACTCAGAGA AGTCAGAGGT CTCTGGAGCA 
GCTGCCAGTG GTTGTTCTGG TTCTTCCACA GTCTTGACTC CCAGCAAGCT GAGCTCCAGT 
CCAGAAGGGC CTGTGTCATT GGCCCAGCCC CCACTGATCC TGCAGCCTCT GGCTTCCCCA 
CTGCAAGTGG GTGTGCCACC CATGACATTA CCTATCATTC TAAATCCAGC CCTCATTGAG 
GCAACTTCAC CTGTGCCTCT GCTAGCTGCC CCTCGCCCCA CTGACTCTAT GCCCACTTCT 
GAAGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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GBF1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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