LINJ_25_1790 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_25_1790
Entrez Gene ID	5069645
Full Name	putative pyruvate dehydrogenase E1 beta subunit
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001466173.1	XP_001466210.1	putative pyruvate dehydrogenase E1 beta subunit

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_25_1790 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_25_1790 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc03275	XM_001466173.1 Latest version!	Leishmania infantum JPCM5 putative pyruvate dehydrogenase E1 beta subunit partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_25_1790 ( XM_001466173.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001466210.1 Leishmania infantum JPCM5 putative pyruvate dehydrogenase E1 beta subunit partial mRNA.

Price & Availability↑

CloneID	OLc03275
Clone ID Related Accession (Same CDS sequence)	XM_001466173.1
Accession Version	XM_001466173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative pyruvate dehydrogenase E1 beta subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009409). COMPLETENESS: incomplete on both ends.

ATGCGCCGCT TCGCCTCCAG AGCTCTGCTC TCTGCGTCCG CCGCCATGGC GGCGCGCTGC 
GCCACAACCA ACATGACGGT GCGCGATGCG ATCCACTCGG CCCTCGACGA GGAGCTCGCC 
CGCGAAGAGA AGGTCTTCGT GATTGGTGAG GAGGTCGGTC AATACCAGGG TGCCTACAAG 
GTGACCAAGG GCCTTGTGGA CAAGTATGGC AAGGACCGCA TCATCGACAT GCCCATCACA 
GAGCACGGCT TCGCCGGCAT GGCCGTTGGC GCCGCTCTCA GTGGCCTGCG CCCCGTGTGC 
GAGTTCATGA CCTTCAACTT CGCCATGCAG GCTATTGATC AGATTGTCAA CTCGGCCGGC 
AAGAGCCTGT ATATGTCTGG TGGCCAGATG AAGTGCCCCA TAGTCTTTCG CGGTCCCAAC 
GGTGCGTCGG CTGGTGTAGG TGCCCAGCAT AGTCAGTGCT TCGGCCCGTG GTACGCGTCC 
GTGCCCGGCC TGAAGGTAAT TGCGCCGTAC AACTGCGAGG ATGCCCGCGG CATGATCAAG 
GCGGCCATCC GCGACGACAA CGCCGTGGTG GTGCTCGAGC ACGAGCTGCT GTACAGCGAG 
TCCTTCCCCG TCACAGACGA GGTAGCGGAC AAGAACTTTG TGATCCCGTT CGGCAAGGCG 
AAGATTGAGC GCGAGGGCAA GGACATTACC CTCATCGGCT TCTCTCGCGG TGTGGATCTG 
TGCTTGAAGG CGGCTGAGAA GCTCGCCGCA GAGGGTGTGC AGGCGGAGGT GATCAACCTT 
CGCTCCCTGC GCCCGCTCGA CCGTCACACC ATCCTCAGCT CCATCAAGAA GACGCACCGT 
GCAGTCACAG TCGATGAGTC CTTCCCTGTG TGCAACATTG GTGCTGAGAT CTGCGCCTGT 
GTCATGGAGA GCGACACGTT CGACTACCTC GATGCCCCGA TCGAGCGCGT GTCGTGCGCC 
GACTGTCCGA CACCGTACAG CAAGGATATC GAGATGGCCT CGCAGCCGCA GGTGGCCGAC 
GTCATGGCCG CTGCCAAGCG CGTACTCAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001466210.1
CDS	1..1053
Translation	MRRFASRALLSASAAMAARCATTNMTVRDAIHSALDEELAREEKVFVIGEEVGQYQGAYKVTKGLVDKYGKDRIIDMPITEHGFAGMAVGAALSGLRPVCEFMTFNFAMQAIDQIVNSAGKSLYMSGGQMKCPIVFRGPNGASAGVGAQHSQCFGPWYASVPGLKVIAPYNCEDARGMIKAAIRDDNAVVVLEHELLYSESFPVTDEVADKNFVIPFGKAKIEREGKDITLIGFSRGVDLCLKAAEKLAAEGVQAEVINLRSLRPLDRHTILSSIKKTHRAVTVDESFPVCNIGAEICACVMESDTFDYLDAPIERVSCADCPTPYSKDIEMASQPQVADVMAAAKRVLS

Target ORF information:

RefSeq Version	XM_001466173.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative pyruvate dehydrogenase E1 beta subunit partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001466173.1

ORF Insert Sequence:

ATGCGCCGCT TCGCCTCCAG AGCTCTGCTC TCTGCGTCCG CCGCCATGGC GGCGCGCTGC 
GCCACAACCA ACATGACGGT GCGCGATGCG ATCCACTCGG CCCTCGACGA GGAGCTCGCC 
CGCGAAGAGA AGGTCTTCGT GATTGGTGAG GAGGTCGGTC AATACCAGGG TGCCTACAAG 
GTGACCAAGG GCCTTGTGGA CAAGTATGGC AAGGACCGCA TCATCGACAT GCCCATCACA 
GAGCACGGCT TCGCCGGCAT GGCCGTTGGC GCCGCTCTCA GTGGCCTGCG CCCCGTGTGC 
GAGTTCATGA CCTTCAACTT CGCCATGCAG GCTATTGATC AGATTGTCAA CTCGGCCGGC 
AAGAGCCTGT ATATGTCTGG TGGCCAGATG AAGTGCCCCA TAGTCTTTCG CGGTCCCAAC 
GGTGCGTCGG CTGGTGTAGG TGCCCAGCAT AGTCAGTGCT TCGGCCCGTG GTACGCGTCC 
GTGCCCGGCC TGAAGGTAAT TGCGCCGTAC AACTGCGAGG ATGCCCGCGG CATGATCAAG 
GCGGCCATCC GCGACGACAA CGCCGTGGTG GTGCTCGAGC ACGAGCTGCT GTACAGCGAG 
TCCTTCCCCG TCACAGACGA GGTAGCGGAC AAGAACTTTG TGATCCCGTT CGGCAAGGCG 
AAGATTGAGC GCGAGGGCAA GGACATTACC CTCATCGGCT TCTCTCGCGG TGTGGATCTG 
TGCTTGAAGG CGGCTGAGAA GCTCGCCGCA GAGGGTGTGC AGGCGGAGGT GATCAACCTT 
CGCTCCCTGC GCCCGCTCGA CCGTCACACC ATCCTCAGCT CCATCAAGAA GACGCACCGT 
GCAGTCACAG TCGATGAGTC CTTCCCTGTG TGCAACATTG GTGCTGAGAT CTGCGCCTGT 
GTCATGGAGA GCGACACGTT CGACTACCTC GATGCCCCGA TCGAGCGCGT GTCGTGCGCC 
GACTGTCCGA CACCGTACAG CAAGGATATC GAGATGGCCT CGCAGCCGCA GGTGGCCGAC 
GTCATGGCCG CTGCCAAGCG CGTACTCAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_25_1790 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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