PAPPA2 cDNA ORF clone, Felis catus(Domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	PAPPA2
Entrez Gene ID	101098353
Full Name	pappalysin 2
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023248030.1	XP_023103798.1	pappalysin-2
XM_019821662.1	XP_019677221.1	pappalysin-2 isoform X2
XM_019821661.1	XP_019677220.1	pappalysin-2 isoform X1
XM_019821663.1	XP_019677222.1	pappalysin-2 isoform X3
XM_003999221.4	XP_003999270.1	pappalysin-2 isoform X1

Pathways from BioSystems
Gene Ontology

The following PAPPA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAPPA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb33824	XM_019821661.1 Latest version!	Felis catus pappalysin 2 (PAPPA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb33824	XM_003999221.4 Latest version!	Felis catus pappalysin 2 (PAPPA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb56298	XM_019821662.1 Latest version!	Felis catus pappalysin 2 (PAPPA2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb56299	XM_019821663.1 Latest version!	Felis catus pappalysin 2 (PAPPA2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb68435	XM_023248030.1 Latest version!	Felis catus pappalysin 2 (PAPPA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PAPPA2 ( XM_019821661.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019677220.1 Felis catus pappalysin 2 (PAPPA2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OFb33824
Clone ID Related Accession (Same CDS sequence)	XM_019821661.1 , XM_003999221.4
Accession Version	XM_019821661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	pappalysin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGCC CAAAGATCCT GAGAATAAGC CTGGTGATTC TGGCCGGGTG GGCGCTCGGC 
ACTGCCGGCT CTGAGCCGGG CTGGACACGC AAGAGGTCCC TGGCTCAGAG GGGACGCCTG 
AACCAGGTGC TGTCGGAAGG AGAACGCTGT TGGCTGGGGG CCAGGCCGCC AAGACCCAGA 
GCCGCTCCCC AGCATCATCT TTTTGGGGTC TACCCCAGCA GGCCTGGGGA CTACCTGAGG 
CCCTACCCCA TGGGGGGGCA GGGAACCCGC CCCGCGGGAC GCAGTGAGCC AGCTGCCACG 
GGGGCTGCGA GCCCAGCTGG AGGTGTGGGG CTGAGGGGCA CAGCTGAGCA GCCAGCCGCC 
TCGTGGGACA GGGATGGTCC CGCTGGGCGG TCTGAGCTGC GGGGAGACGA TGACACTTAT 
CTTGGTGATG GAGGATCCAA GGAGTCTCTA GGTGAGGCTG GGACTCGTGA ACGCTCGGCC 
ACGGCTGCCA CGGCTGCTAC CACCTTCGTG GGCCCAAAGG AACCCAAACC AGAGACCCAA 
AGCAAGGGCC GGGCCAAGAC CAGGCGTCGT CGCCAGGTGG TGAAGGGGCA GGTAGGGGCC 
GTGCAGGGAG AGCCCGGTGT CTCTCCCCAG CGTCTCTGGC CGCGGCCGAA GCATCCCCTT 
GTGCACGGGG TCAGAATGAG TCTGTCGGAG GACGGCATCC AGAAGGGTGG AGGGGACCCC 
TCCTGGGAAG CAGAGTCCCA GGGAGGACTG CCTGTCTTGT ATTTCTCCGG GAGGCGGGGG 
CGGCTCCTGC TGCGTCCAGA AGTGCTGGCT GAGATTCCCC GGGAGGCGTT CACAGTGGAA 
GCCTGGGTGA GACCGGAGGG AGGACAGAAC AACCCAGCCA TCATTGCAGG TGTGTTTGAT 
AACTGCTCCC ACACTGTCAG TGACAAGGGC TGGGCCCTAG GGATCCGCTC AGGGAAGGAC 
ATAGGAAGGC GAGACGCTCG CTTCTTCTTC TCCCTTCGCA CCGACCGTGT GAAGAAAGCC 
ACCATTGTGA CTGGCAACAG TCGCTACCAA CCAGGCACAT GGACACACGT GGCAGCCACC 
TATGATGGAC ATCGTATGGC TCTGTATGTG GATGGCACTC AGGTGGCCAG TAGCCCAGAG 
CAATCTGGTC CCCTGAACAG CCCCTTCATG GCATCCTGTC GCTCTTTGCT TCTGGGGGGG 
GATAGCTCTG AGGACGGCCG CTATTTTCGT GGACACCTGG GCACCCTGGT CTTCTGGTCG 
ACTGCCCTCC CGCAAAGCCA TCTCCGGCAC AGTCCTCAGC GTCCAGCTGG AGTGGAAGAA 
TTGGCCTCCC TCATCCTGAC AGCCAGTTTT GAGCCCTTGG AAGAACAGTG GGCCCCCTTT 
AGAGATGGGG AGTTCCCACG GCGTGAGGTT CTGCATGGCT TTGAGCCTGA GCCTGAGATT 
GTGTCACCTC TGAAGCCCCC ACTCTGTGGA CGGACGGCTT GTGACAACGT GGAACTGATC 
TCCCACTACA ACGGGCACCG GCCCCTCCAG GGAGAGAAGG TGATCCGCTA CCGAGTGGTA 
AACATCTGTG ACGATGAGGG CCTGAACCCC ACTGTGAGCG AGGAGCAGAT CAGCCGGCAG 
CACAAGGCGC TGAACCATGC CTTCAGCCGC TACAACATCA GCTGGCAGCT GAGCGTCCAC 
CAGGTCCACA ACTCCACCCT GCGCCACCGG GTTGTGCTTG TGAACTGCGA GCCCAGCAAG 
ATTGGCAATG ACCACTGTGA CCCTGAGTGT GAGCACCCAC TCACAGGCTA TGACGGGGGC 
GACTGCCGCC TACATGGCCG GTGCTACTCC TGGAACCGCA GGGACGGGCT CTGCCACGTG 
GAGTGCAACA ACATGCTGAA CGACTTTGAC GACGGGGACT GCTGCGACCC CGAGGTGGCC 
GATGTGCACA AGACCTGCTT TGACCCCGAC TCACCCAAGA GGGCATACAT GAGTGTGAAG 
GAGCTGAAGG AGGCACTGCA GCTCAACAGT ACTCACTTCC TCAATGTCTA CTTTGCCAGC 
TCCGTGCGGG AAGACCTTGC AGGCGCTGCC ACCTGGCCTT GGGACAAGGA AGCCATCAGT 
CACCTGGGTG GCGTTGTCCT CAACCCAGCC TATTATGGCA TGCCTGGCCA TACCAACATT 
ATGATCCACG AGGTAGGGCA TGTCCTGGGA CTCTACCATG TCTTCAAAGG GGTCAGTGAG 
AGGGAATCCT GTGATGACCC CTGTAGGGAG ACAGTGCCAT CCATGGATAC GGGAGACCTC 
TGTGCCGACA CCGCCCCTAC TCCCAAGAGT AAGCTGTGCC AGGACCCAGA GCCTACCAAC 
GATACCTGTG GCTTCACCCA CTTCCCGGGG GCTCCGTTCA GCAACTACAT GAGCTATACA 
GATGATGATT GCACCGACAG CTTCACCCCT AACCAGGTGG CCCGGATGCA TTGCTATTTG 
GACCTTGTGT ATCAGCAGTG GAGTCAAAGC CGGAAGCCCA CCCCCATTCC CATCCCACCC 
ATGGTCATCG GGCAGACCCA CACCTCCCTC ACCATCCACT GGCTTCCTCC AATCAGTGGG 
GTTGTACACG ACAGGGTCCC AGGCAGCGTG TGTGGTGCCT GCACTGAAGA TGGGACATTC 
CGTCAGTACG TGCACGCAGC TTCCTCCCGG CGTGTGTGTG ACTCCTCAGG TTACTGGACT 
CCAGAGGAGG CTGTGGGGCC TCCAGACGTG GACCAGCCCT GTGAGCCGAG CTTGCAGGCC 
TGGAGTCCCG AGCTCCACCT GTACCATATG AACATGACGG TCCCCTGCCC TTCGGAAGGC 
TGTAGCCTGG AGCTGCTCTT CCAGCACCCG GTCCAAGCTG ACACCCTTAC CCTATGGGTC 
ACCTACCTCT CCATGGACTC CTCCCAGGCA CTCTTTGACA CGGAGATCTT GCTGGAACAC 
CAGGAGTCTG TGCACCTGGG CCCCTTAGAC ACTTTCTGTG ACACCCCACT CACTATCAAA 
CTGCATGTGG ATGGGAAGGT CTCTGGGGTG AAGGTCTACA CCTTTGATGA GCGGATGGAG 
ATTGACGCAG CTCTGCTAAC TTCCCAGCCC CATAGTCCCC TGTGCTCTGG CTGCAGGCCT 
GTGAGGTACC AGGTTCTTCG TGAGCCCCCA TTTGCCAATG GCTTGCCCAT GGTGGTGACA 
AATCCTCACA GGAAGTTCAC GGACGTGGAG GTCACACCTG GGCAGCTGTA TCGGTACCAA 
GTTCAAGCTG GAGCTGGAAC AGAACTAGGA GAAGCCTCGC CTCCGCTGAG CCACATCCAT 
GGAGCTCCTT ACTGTGGAGA CGGGAAGGTG GCAGGGAGCC TGGGAGAAGA ATGTGATGAT 
GGGGACCTTC TGAGCGGAGA TGGCTGCTCA AGGGCGTGCC AGCTGGAGGA AGGCTTCCAC 
TGCGCAGGGG AGCCAAGCCT ATGTTACATA TATGAGGGAG ATGGGATATG TGAACCTTTT 
GAGAAGGAAA CCAGCATCAT AGACTGTGGC CTCTATACTC CCAAAGGATA TGTGGATCAG 
TGGGCCACCC AAGCTTATTC CTCTCATGAA GACAAGAAGT GTCCTGCTTC CTTGGTAACT 
GGAGAACCTC ATTCCATGAT TTGCACGTCA TACCATCCAG ACTTACCAGA GCACCGTCCT 
CTCACTGGCT GGTTTCCCTG TGTCACCAAT GGAAACAGAC CTCAGGATAA AAGGAGTGAG 
CAGACAGAAG GTAGCCTGGA GAGGGAGGAG GAGGTTTGGC TCAAAGTGTG TTTCAGTAGA 
CCAGGAGTGG CCACGACAAT TTTCATTTTC TTGACATCTG ATGGCCCGGC ACTTGGAGAA 
CATCGGCGGC CAACAGTGAC TGTTCACCTG ACTGATGTCA GTGGGAACAA CCACTCTCTT 
GGAACCTATG AACTGTCATG CCAGCATAAC CCGCTGGTTA TCAATGTGAG CCATCACCCG 
AATGTCCTCT CCCACCATAC CTCTTCAGTG CTGCTGAATT TCTCATCCCC ATTGGTGGGC 
ATCTCAGCGG TGGCTCTAAG GACATCCTCC CACATAAGTT CATCAACTCT CAATAACTGC 
ATCCCAGAGC ATGAGGGACA AAATCATCAG GGACAGAGCT GTGTCCACCG GCCCTGTGGG 
GAGCAGGGTA GCTGTGCGCC ATTGCTGCTT GATCATGCGG ATATGGTGAA CTGTACCTCT 
GGTGGCCCAG GTCACATGAA GTGTGCTATC ACCTGTCAAA GGGGGTTTGC CCTTCAGGCC 
AGCAGCGGGC AATACCTCAG GCCCGTGCAG AAGGAAATTC TGCTCACCTG TTCCTCTGGG 
CACTGGGACC GGGCTGTGAG CTGCATGCCC CTTGATTGTG GCGTTCCCGA CCCATCTTTG 
GTGAACTACG CAAACTTCTC CTGCTCAGAG GGAACAGACT TTCTGAAGCG CTGTTCAATC 
TCCTGTGTCC CACCAGCCAA GCTCCAAGGA CTGAACCCAT GGCTGACTTG TCTTGAAGAC 
GGGCTCTGGT CCCTCCCGGA AGCCTACTGC AAGTTGGAGT GTGACGCCCC CCCTGTAATC 
CCGAATGCCA GCCTGCTCTT GCCTCACTGC CTCCAGGGCA ACCATGACGT GGGCTCCGTC 
TGTAGATACG AATGCAAACC AGGCTACTAT GCCGAGCCAA GTGCCGAGGG TAAAGTCAGG 
AGCAATTTCC TGAAGATACA GTGCCTGGAG GGTGGCATCT GGGAGCAAGG CAGCTGCGTT 
CCAGTGGTGT GTGAGCCCCC TTCCCCTGTC TTTGAAGGCA TGTATGAATG TACCAATGGC 
TTTGAACTCA ACAGCCGGTG TGTGCTCAGC TGTAACCAGG AAAGCGGAAG GGTTCCTATC 
CTCTGCACTA AGGAGGGACT GTGGACTGAG GAGTTCAAGT TGTGTGAGAA CCTGCAAGGG 
GAGTGCCCGC CACCCCCGTC GGAGCTGAAT TTTGTGGAGT ACAAGTGTGA ACAGGGATAC 
GGGATTGGTG CTGTGTGTTC CCCATCATGT GTGATTCCCC CTAGTGACCC CGTAGTTCTG 
CCTGAGAACG TCACTGCTGA CACGCTGGAG CACTGGATGG AACCTGTCAA AGTCCAGAGC 
ATTGTGTGCA CTGGACAGCG TCAATGGCAC CCACCCCCCT TCCTCATCCA CTGCATCCAG 
TCGTGTGAGC CTTTCCAAGC CGATGGGTGG TGCGATACCA TCAACAACCG GGCCTACTGC 
CACTATGATG GGGGAGACTG CTGCTCTTCT ACTCTGTCCT CCAAGAAGGT CATTCCATTT 
GCTGCTGACT GTGACCTGGA TGAATGCACC TGCCGGGACC CCAAGGCTGA AGAAAATCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019677220.1
CDS	1007..6349
Translation	MMGPKILRISLVILAGWALGTAGSEPGWTRKRSLAQRGRLNQVLSEGERCWLGARPPRPRAAPQHHLFGVYPSRPGDYLRPYPMGGQGTRPAGRSEPAATGAASPAGGVGLRGTAEQPAASWDRDGPAGRSELRGDDDTYLGDGGSKESLGEAGTRERSATAATAATTFVGPKEPKPETQSKGRAKTRRRRQVVKGQVGAVQGEPGVSPQRLWPRPKHPLVHGVRMSLSEDGIQKGGGDPSWEAESQGGLPVLYFSGRRGRLLLRPEVLAEIPREAFTVEAWVRPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDIGRRDARFFFSLRTDRVKKATIVTGNSRYQPGTWTHVAATYDGHRMALYVDGTQVASSPEQSGPLNSPFMASCRSLLLGGDSSEDGRYFRGHLGTLVFWSTALPQSHLRHSPQRPAGVEELASLILTASFEPLEEQWAPFRDGEFPRREVLHGFEPEPEIVSPLKPPLCGRTACDNVELISHYNGHRPLQGEKVIRYRVVNICDDEGLNPTVSEEQISRQHKALNHAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLHGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVADVHKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGAATWPWDKEAISHLGGVVLNPAYYGMPGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMDTGDLCADTAPTPKSKLCQDPEPTNDTCGFTHFPGAPFSNYMSYTDDDCTDSFTPNQVARMHCYLDLVYQQWSQSRKPTPIPIPPMVIGQTHTSLTIHWLPPISGVVHDRVPGSVCGACTEDGTFRQYVHAASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLYHMNMTVPCPSEGCSLELLFQHPVQADTLTLWVTYLSMDSSQALFDTEILLEHQESVHLGPLDTFCDTPLTIKLHVDGKVSGVKVYTFDERMEIDAALLTSQPHSPLCSGCRPVRYQVLREPPFANGLPMVVTNPHRKFTDVEVTPGQLYRYQVQAGAGTELGEASPPLSHIHGAPYCGDGKVAGSLGEECDDGDLLSGDGCSRACQLEEGFHCAGEPSLCYIYEGDGICEPFEKETSIIDCGLYTPKGYVDQWATQAYSSHEDKKCPASLVTGEPHSMICTSYHPDLPEHRPLTGWFPCVTNGNRPQDKRSEQTEGSLEREEEVWLKVCFSRPGVATTIFIFLTSDGPALGEHRRPTVTVHLTDVSGNNHSLGTYELSCQHNPLVINVSHHPNVLSHHTSSVLLNFSSPLVGISAVALRTSSHISSSTLNNCIPEHEGQNHQGQSCVHRPCGEQGSCAPLLLDHADMVNCTSGGPGHMKCAITCQRGFALQASSGQYLRPVQKEILLTCSSGHWDRAVSCMPLDCGVPDPSLVNYANFSCSEGTDFLKRCSISCVPPAKLQGLNPWLTCLEDGLWSLPEAYCKLECDAPPVIPNASLLLPHCLQGNHDVGSVCRYECKPGYYAEPSAEGKVRSNFLKIQCLEGGIWEQGSCVPVVCEPPSPVFEGMYECTNGFELNSRCVLSCNQESGRVPILCTKEGLWTEEFKLCENLQGECPPPPSELNFVEYKCEQGYGIGAVCSPSCVIPPSDPVVLPENVTADTLEHWMEPVKVQSIVCTGQRQWHPPPFLIHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_019821661.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus pappalysin 2 (PAPPA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019821661.1

ORF Insert Sequence:

ATGATGGGCC CAAAGATCCT GAGAATAAGC CTGGTGATTC TGGCCGGGTG GGCGCTCGGC 
ACTGCCGGCT CTGAGCCGGG CTGGACACGC AAGAGGTCCC TGGCTCAGAG GGGACGCCTG 
AACCAGGTGC TGTCGGAAGG AGAACGCTGT TGGCTGGGGG CCAGGCCGCC AAGACCCAGA 
GCCGCTCCCC AGCATCATCT TTTTGGGGTC TACCCCAGCA GGCCTGGGGA CTACCTGAGG 
CCCTACCCCA TGGGGGGGCA GGGAACCCGC CCCGCGGGAC GCAGTGAGCC AGCTGCCACG 
GGGGCTGCGA GCCCAGCTGG AGGTGTGGGG CTGAGGGGCA CAGCTGAGCA GCCAGCCGCC 
TCGTGGGACA GGGATGGTCC CGCTGGGCGG TCTGAGCTGC GGGGAGACGA TGACACTTAT 
CTTGGTGATG GAGGATCCAA GGAGTCTCTA GGTGAGGCTG GGACTCGTGA ACGCTCGGCC 
ACGGCTGCCA CGGCTGCTAC CACCTTCGTG GGCCCAAAGG AACCCAAACC AGAGACCCAA 
AGCAAGGGCC GGGCCAAGAC CAGGCGTCGT CGCCAGGTGG TGAAGGGGCA GGTAGGGGCC 
GTGCAGGGAG AGCCCGGTGT CTCTCCCCAG CGTCTCTGGC CGCGGCCGAA GCATCCCCTT 
GTGCACGGGG TCAGAATGAG TCTGTCGGAG GACGGCATCC AGAAGGGTGG AGGGGACCCC 
TCCTGGGAAG CAGAGTCCCA GGGAGGACTG CCTGTCTTGT ATTTCTCCGG GAGGCGGGGG 
CGGCTCCTGC TGCGTCCAGA AGTGCTGGCT GAGATTCCCC GGGAGGCGTT CACAGTGGAA 
GCCTGGGTGA GACCGGAGGG AGGACAGAAC AACCCAGCCA TCATTGCAGG TGTGTTTGAT 
AACTGCTCCC ACACTGTCAG TGACAAGGGC TGGGCCCTAG GGATCCGCTC AGGGAAGGAC 
ATAGGAAGGC GAGACGCTCG CTTCTTCTTC TCCCTTCGCA CCGACCGTGT GAAGAAAGCC 
ACCATTGTGA CTGGCAACAG TCGCTACCAA CCAGGCACAT GGACACACGT GGCAGCCACC 
TATGATGGAC ATCGTATGGC TCTGTATGTG GATGGCACTC AGGTGGCCAG TAGCCCAGAG 
CAATCTGGTC CCCTGAACAG CCCCTTCATG GCATCCTGTC GCTCTTTGCT TCTGGGGGGG 
GATAGCTCTG AGGACGGCCG CTATTTTCGT GGACACCTGG GCACCCTGGT CTTCTGGTCG 
ACTGCCCTCC CGCAAAGCCA TCTCCGGCAC AGTCCTCAGC GTCCAGCTGG AGTGGAAGAA 
TTGGCCTCCC TCATCCTGAC AGCCAGTTTT GAGCCCTTGG AAGAACAGTG GGCCCCCTTT 
AGAGATGGGG AGTTCCCACG GCGTGAGGTT CTGCATGGCT TTGAGCCTGA GCCTGAGATT 
GTGTCACCTC TGAAGCCCCC ACTCTGTGGA CGGACGGCTT GTGACAACGT GGAACTGATC 
TCCCACTACA ACGGGCACCG GCCCCTCCAG GGAGAGAAGG TGATCCGCTA CCGAGTGGTA 
AACATCTGTG ACGATGAGGG CCTGAACCCC ACTGTGAGCG AGGAGCAGAT CAGCCGGCAG 
CACAAGGCGC TGAACCATGC CTTCAGCCGC TACAACATCA GCTGGCAGCT GAGCGTCCAC 
CAGGTCCACA ACTCCACCCT GCGCCACCGG GTTGTGCTTG TGAACTGCGA GCCCAGCAAG 
ATTGGCAATG ACCACTGTGA CCCTGAGTGT GAGCACCCAC TCACAGGCTA TGACGGGGGC 
GACTGCCGCC TACATGGCCG GTGCTACTCC TGGAACCGCA GGGACGGGCT CTGCCACGTG 
GAGTGCAACA ACATGCTGAA CGACTTTGAC GACGGGGACT GCTGCGACCC CGAGGTGGCC 
GATGTGCACA AGACCTGCTT TGACCCCGAC TCACCCAAGA GGGCATACAT GAGTGTGAAG 
GAGCTGAAGG AGGCACTGCA GCTCAACAGT ACTCACTTCC TCAATGTCTA CTTTGCCAGC 
TCCGTGCGGG AAGACCTTGC AGGCGCTGCC ACCTGGCCTT GGGACAAGGA AGCCATCAGT 
CACCTGGGTG GCGTTGTCCT CAACCCAGCC TATTATGGCA TGCCTGGCCA TACCAACATT 
ATGATCCACG AGGTAGGGCA TGTCCTGGGA CTCTACCATG TCTTCAAAGG GGTCAGTGAG 
AGGGAATCCT GTGATGACCC CTGTAGGGAG ACAGTGCCAT CCATGGATAC GGGAGACCTC 
TGTGCCGACA CCGCCCCTAC TCCCAAGAGT AAGCTGTGCC AGGACCCAGA GCCTACCAAC 
GATACCTGTG GCTTCACCCA CTTCCCGGGG GCTCCGTTCA GCAACTACAT GAGCTATACA 
GATGATGATT GCACCGACAG CTTCACCCCT AACCAGGTGG CCCGGATGCA TTGCTATTTG 
GACCTTGTGT ATCAGCAGTG GAGTCAAAGC CGGAAGCCCA CCCCCATTCC CATCCCACCC 
ATGGTCATCG GGCAGACCCA CACCTCCCTC ACCATCCACT GGCTTCCTCC AATCAGTGGG 
GTTGTACACG ACAGGGTCCC AGGCAGCGTG TGTGGTGCCT GCACTGAAGA TGGGACATTC 
CGTCAGTACG TGCACGCAGC TTCCTCCCGG CGTGTGTGTG ACTCCTCAGG TTACTGGACT 
CCAGAGGAGG CTGTGGGGCC TCCAGACGTG GACCAGCCCT GTGAGCCGAG CTTGCAGGCC 
TGGAGTCCCG AGCTCCACCT GTACCATATG AACATGACGG TCCCCTGCCC TTCGGAAGGC 
TGTAGCCTGG AGCTGCTCTT CCAGCACCCG GTCCAAGCTG ACACCCTTAC CCTATGGGTC 
ACCTACCTCT CCATGGACTC CTCCCAGGCA CTCTTTGACA CGGAGATCTT GCTGGAACAC 
CAGGAGTCTG TGCACCTGGG CCCCTTAGAC ACTTTCTGTG ACACCCCACT CACTATCAAA 
CTGCATGTGG ATGGGAAGGT CTCTGGGGTG AAGGTCTACA CCTTTGATGA GCGGATGGAG 
ATTGACGCAG CTCTGCTAAC TTCCCAGCCC CATAGTCCCC TGTGCTCTGG CTGCAGGCCT 
GTGAGGTACC AGGTTCTTCG TGAGCCCCCA TTTGCCAATG GCTTGCCCAT GGTGGTGACA 
AATCCTCACA GGAAGTTCAC GGACGTGGAG GTCACACCTG GGCAGCTGTA TCGGTACCAA 
GTTCAAGCTG GAGCTGGAAC AGAACTAGGA GAAGCCTCGC CTCCGCTGAG CCACATCCAT 
GGAGCTCCTT ACTGTGGAGA CGGGAAGGTG GCAGGGAGCC TGGGAGAAGA ATGTGATGAT 
GGGGACCTTC TGAGCGGAGA TGGCTGCTCA AGGGCGTGCC AGCTGGAGGA AGGCTTCCAC 
TGCGCAGGGG AGCCAAGCCT ATGTTACATA TATGAGGGAG ATGGGATATG TGAACCTTTT 
GAGAAGGAAA CCAGCATCAT AGACTGTGGC CTCTATACTC CCAAAGGATA TGTGGATCAG 
TGGGCCACCC AAGCTTATTC CTCTCATGAA GACAAGAAGT GTCCTGCTTC CTTGGTAACT 
GGAGAACCTC ATTCCATGAT TTGCACGTCA TACCATCCAG ACTTACCAGA GCACCGTCCT 
CTCACTGGCT GGTTTCCCTG TGTCACCAAT GGAAACAGAC CTCAGGATAA AAGGAGTGAG 
CAGACAGAAG GTAGCCTGGA GAGGGAGGAG GAGGTTTGGC TCAAAGTGTG TTTCAGTAGA 
CCAGGAGTGG CCACGACAAT TTTCATTTTC TTGACATCTG ATGGCCCGGC ACTTGGAGAA 
CATCGGCGGC CAACAGTGAC TGTTCACCTG ACTGATGTCA GTGGGAACAA CCACTCTCTT 
GGAACCTATG AACTGTCATG CCAGCATAAC CCGCTGGTTA TCAATGTGAG CCATCACCCG 
AATGTCCTCT CCCACCATAC CTCTTCAGTG CTGCTGAATT TCTCATCCCC ATTGGTGGGC 
ATCTCAGCGG TGGCTCTAAG GACATCCTCC CACATAAGTT CATCAACTCT CAATAACTGC 
ATCCCAGAGC ATGAGGGACA AAATCATCAG GGACAGAGCT GTGTCCACCG GCCCTGTGGG 
GAGCAGGGTA GCTGTGCGCC ATTGCTGCTT GATCATGCGG ATATGGTGAA CTGTACCTCT 
GGTGGCCCAG GTCACATGAA GTGTGCTATC ACCTGTCAAA GGGGGTTTGC CCTTCAGGCC 
AGCAGCGGGC AATACCTCAG GCCCGTGCAG AAGGAAATTC TGCTCACCTG TTCCTCTGGG 
CACTGGGACC GGGCTGTGAG CTGCATGCCC CTTGATTGTG GCGTTCCCGA CCCATCTTTG 
GTGAACTACG CAAACTTCTC CTGCTCAGAG GGAACAGACT TTCTGAAGCG CTGTTCAATC 
TCCTGTGTCC CACCAGCCAA GCTCCAAGGA CTGAACCCAT GGCTGACTTG TCTTGAAGAC 
GGGCTCTGGT CCCTCCCGGA AGCCTACTGC AAGTTGGAGT GTGACGCCCC CCCTGTAATC 
CCGAATGCCA GCCTGCTCTT GCCTCACTGC CTCCAGGGCA ACCATGACGT GGGCTCCGTC 
TGTAGATACG AATGCAAACC AGGCTACTAT GCCGAGCCAA GTGCCGAGGG TAAAGTCAGG 
AGCAATTTCC TGAAGATACA GTGCCTGGAG GGTGGCATCT GGGAGCAAGG CAGCTGCGTT 
CCAGTGGTGT GTGAGCCCCC TTCCCCTGTC TTTGAAGGCA TGTATGAATG TACCAATGGC 
TTTGAACTCA ACAGCCGGTG TGTGCTCAGC TGTAACCAGG AAAGCGGAAG GGTTCCTATC 
CTCTGCACTA AGGAGGGACT GTGGACTGAG GAGTTCAAGT TGTGTGAGAA CCTGCAAGGG 
GAGTGCCCGC CACCCCCGTC GGAGCTGAAT TTTGTGGAGT ACAAGTGTGA ACAGGGATAC 
GGGATTGGTG CTGTGTGTTC CCCATCATGT GTGATTCCCC CTAGTGACCC CGTAGTTCTG 
CCTGAGAACG TCACTGCTGA CACGCTGGAG CACTGGATGG AACCTGTCAA AGTCCAGAGC 
ATTGTGTGCA CTGGACAGCG TCAATGGCAC CCACCCCCCT TCCTCATCCA CTGCATCCAG 
TCGTGTGAGC CTTTCCAAGC CGATGGGTGG TGCGATACCA TCAACAACCG GGCCTACTGC 
CACTATGATG GGGGAGACTG CTGCTCTTCT ACTCTGTCCT CCAAGAAGGT CATTCCATTT 
GCTGCTGACT GTGACCTGGA TGAATGCACC TGCCGGGACC CCAAGGCTGA AGAAAATCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_003999221.4 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003999270.1 Felis catus pappalysin 2 (PAPPA2), transcript variant X1, mRNA.

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CloneID	OFb33824
Clone ID Related Accession (Same CDS sequence)	XM_019821661.1 , XM_003999221.4
Accession Version	XM_003999221.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	pappalysin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003999221.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGCC CAAAGATCCT GAGAATAAGC CTGGTGATTC TGGCCGGGTG GGCGCTCGGC 
ACTGCCGGCT CTGAGCCGGG CTGGACACGC AAGAGGTCCC TGGCTCAGAG GGGACGCCTG 
AACCAGGTGC TGTCGGAAGG AGAACGCTGT TGGCTGGGGG CCAGGCCGCC AAGACCCAGA 
GCCGCTCCCC AGCATCATCT TTTTGGGGTC TACCCCAGCA GGCCTGGGGA CTACCTGAGG 
CCCTACCCCA TGGGGGGGCA GGGAACCCGC CCCGCGGGAC GCAGTGAGCC AGCTGCCACG 
GGGGCTGCGA GCCCAGCTGG AGGTGTGGGG CTGAGGGGCA CAGCTGAGCA GCCAGCCGCC 
TCGTGGGACA GGGATGGTCC CGCTGGGCGG TCTGAGCTGC GGGGAGACGA TGACACTTAT 
CTTGGTGATG GAGGATCCAA GGAGTCTCTA GGTGAGGCTG GGACTCGTGA ACGCTCGGCC 
ACGGCTGCCA CGGCTGCTAC CACCTTCGTG GGCCCAAAGG AACCCAAACC AGAGACCCAA 
AGCAAGGGCC GGGCCAAGAC CAGGCGTCGT CGCCAGGTGG TGAAGGGGCA GGTAGGGGCC 
GTGCAGGGAG AGCCCGGTGT CTCTCCCCAG CGTCTCTGGC CGCGGCCGAA GCATCCCCTT 
GTGCACGGGG TCAGAATGAG TCTGTCGGAG GACGGCATCC AGAAGGGTGG AGGGGACCCC 
TCCTGGGAAG CAGAGTCCCA GGGAGGACTG CCTGTCTTGT ATTTCTCCGG GAGGCGGGGG 
CGGCTCCTGC TGCGTCCAGA AGTGCTGGCT GAGATTCCCC GGGAGGCGTT CACAGTGGAA 
GCCTGGGTGA GACCGGAGGG AGGACAGAAC AACCCAGCCA TCATTGCAGG TGTGTTTGAT 
AACTGCTCCC ACACTGTCAG TGACAAGGGC TGGGCCCTAG GGATCCGCTC AGGGAAGGAC 
ATAGGAAGGC GAGACGCTCG CTTCTTCTTC TCCCTTCGCA CCGACCGTGT GAAGAAAGCC 
ACCATTGTGA CTGGCAACAG TCGCTACCAA CCAGGCACAT GGACACACGT GGCAGCCACC 
TATGATGGAC ATCGTATGGC TCTGTATGTG GATGGCACTC AGGTGGCCAG TAGCCCAGAG 
CAATCTGGTC CCCTGAACAG CCCCTTCATG GCATCCTGTC GCTCTTTGCT TCTGGGGGGG 
GATAGCTCTG AGGACGGCCG CTATTTTCGT GGACACCTGG GCACCCTGGT CTTCTGGTCG 
ACTGCCCTCC CGCAAAGCCA TCTCCGGCAC AGTCCTCAGC GTCCAGCTGG AGTGGAAGAA 
TTGGCCTCCC TCATCCTGAC AGCCAGTTTT GAGCCCTTGG AAGAACAGTG GGCCCCCTTT 
AGAGATGGGG AGTTCCCACG GCGTGAGGTT CTGCATGGCT TTGAGCCTGA GCCTGAGATT 
GTGTCACCTC TGAAGCCCCC ACTCTGTGGA CGGACGGCTT GTGACAACGT GGAACTGATC 
TCCCACTACA ACGGGCACCG GCCCCTCCAG GGAGAGAAGG TGATCCGCTA CCGAGTGGTA 
AACATCTGTG ACGATGAGGG CCTGAACCCC ACTGTGAGCG AGGAGCAGAT CAGCCGGCAG 
CACAAGGCGC TGAACCATGC CTTCAGCCGC TACAACATCA GCTGGCAGCT GAGCGTCCAC 
CAGGTCCACA ACTCCACCCT GCGCCACCGG GTTGTGCTTG TGAACTGCGA GCCCAGCAAG 
ATTGGCAATG ACCACTGTGA CCCTGAGTGT GAGCACCCAC TCACAGGCTA TGACGGGGGC 
GACTGCCGCC TACATGGCCG GTGCTACTCC TGGAACCGCA GGGACGGGCT CTGCCACGTG 
GAGTGCAACA ACATGCTGAA CGACTTTGAC GACGGGGACT GCTGCGACCC CGAGGTGGCC 
GATGTGCACA AGACCTGCTT TGACCCCGAC TCACCCAAGA GGGCATACAT GAGTGTGAAG 
GAGCTGAAGG AGGCACTGCA GCTCAACAGT ACTCACTTCC TCAATGTCTA CTTTGCCAGC 
TCCGTGCGGG AAGACCTTGC AGGCGCTGCC ACCTGGCCTT GGGACAAGGA AGCCATCAGT 
CACCTGGGTG GCGTTGTCCT CAACCCAGCC TATTATGGCA TGCCTGGCCA TACCAACATT 
ATGATCCACG AGGTAGGGCA TGTCCTGGGA CTCTACCATG TCTTCAAAGG GGTCAGTGAG 
AGGGAATCCT GTGATGACCC CTGTAGGGAG ACAGTGCCAT CCATGGATAC GGGAGACCTC 
TGTGCCGACA CCGCCCCTAC TCCCAAGAGT AAGCTGTGCC AGGACCCAGA GCCTACCAAC 
GATACCTGTG GCTTCACCCA CTTCCCGGGG GCTCCGTTCA GCAACTACAT GAGCTATACA 
GATGATGATT GCACCGACAG CTTCACCCCT AACCAGGTGG CCCGGATGCA TTGCTATTTG 
GACCTTGTGT ATCAGCAGTG GAGTCAAAGC CGGAAGCCCA CCCCCATTCC CATCCCACCC 
ATGGTCATCG GGCAGACCCA CACCTCCCTC ACCATCCACT GGCTTCCTCC AATCAGTGGG 
GTTGTACACG ACAGGGTCCC AGGCAGCGTG TGTGGTGCCT GCACTGAAGA TGGGACATTC 
CGTCAGTACG TGCACGCAGC TTCCTCCCGG CGTGTGTGTG ACTCCTCAGG TTACTGGACT 
CCAGAGGAGG CTGTGGGGCC TCCAGACGTG GACCAGCCCT GTGAGCCGAG CTTGCAGGCC 
TGGAGTCCCG AGCTCCACCT GTACCATATG AACATGACGG TCCCCTGCCC TTCGGAAGGC 
TGTAGCCTGG AGCTGCTCTT CCAGCACCCG GTCCAAGCTG ACACCCTTAC CCTATGGGTC 
ACCTACCTCT CCATGGACTC CTCCCAGGCA CTCTTTGACA CGGAGATCTT GCTGGAACAC 
CAGGAGTCTG TGCACCTGGG CCCCTTAGAC ACTTTCTGTG ACACCCCACT CACTATCAAA 
CTGCATGTGG ATGGGAAGGT CTCTGGGGTG AAGGTCTACA CCTTTGATGA GCGGATGGAG 
ATTGACGCAG CTCTGCTAAC TTCCCAGCCC CATAGTCCCC TGTGCTCTGG CTGCAGGCCT 
GTGAGGTACC AGGTTCTTCG TGAGCCCCCA TTTGCCAATG GCTTGCCCAT GGTGGTGACA 
AATCCTCACA GGAAGTTCAC GGACGTGGAG GTCACACCTG GGCAGCTGTA TCGGTACCAA 
GTTCAAGCTG GAGCTGGAAC AGAACTAGGA GAAGCCTCGC CTCCGCTGAG CCACATCCAT 
GGAGCTCCTT ACTGTGGAGA CGGGAAGGTG GCAGGGAGCC TGGGAGAAGA ATGTGATGAT 
GGGGACCTTC TGAGCGGAGA TGGCTGCTCA AGGGCGTGCC AGCTGGAGGA AGGCTTCCAC 
TGCGCAGGGG AGCCAAGCCT ATGTTACATA TATGAGGGAG ATGGGATATG TGAACCTTTT 
GAGAAGGAAA CCAGCATCAT AGACTGTGGC CTCTATACTC CCAAAGGATA TGTGGATCAG 
TGGGCCACCC AAGCTTATTC CTCTCATGAA GACAAGAAGT GTCCTGCTTC CTTGGTAACT 
GGAGAACCTC ATTCCATGAT TTGCACGTCA TACCATCCAG ACTTACCAGA GCACCGTCCT 
CTCACTGGCT GGTTTCCCTG TGTCACCAAT GGAAACAGAC CTCAGGATAA AAGGAGTGAG 
CAGACAGAAG GTAGCCTGGA GAGGGAGGAG GAGGTTTGGC TCAAAGTGTG TTTCAGTAGA 
CCAGGAGTGG CCACGACAAT TTTCATTTTC TTGACATCTG ATGGCCCGGC ACTTGGAGAA 
CATCGGCGGC CAACAGTGAC TGTTCACCTG ACTGATGTCA GTGGGAACAA CCACTCTCTT 
GGAACCTATG AACTGTCATG CCAGCATAAC CCGCTGGTTA TCAATGTGAG CCATCACCCG 
AATGTCCTCT CCCACCATAC CTCTTCAGTG CTGCTGAATT TCTCATCCCC ATTGGTGGGC 
ATCTCAGCGG TGGCTCTAAG GACATCCTCC CACATAAGTT CATCAACTCT CAATAACTGC 
ATCCCAGAGC ATGAGGGACA AAATCATCAG GGACAGAGCT GTGTCCACCG GCCCTGTGGG 
GAGCAGGGTA GCTGTGCGCC ATTGCTGCTT GATCATGCGG ATATGGTGAA CTGTACCTCT 
GGTGGCCCAG GTCACATGAA GTGTGCTATC ACCTGTCAAA GGGGGTTTGC CCTTCAGGCC 
AGCAGCGGGC AATACCTCAG GCCCGTGCAG AAGGAAATTC TGCTCACCTG TTCCTCTGGG 
CACTGGGACC GGGCTGTGAG CTGCATGCCC CTTGATTGTG GCGTTCCCGA CCCATCTTTG 
GTGAACTACG CAAACTTCTC CTGCTCAGAG GGAACAGACT TTCTGAAGCG CTGTTCAATC 
TCCTGTGTCC CACCAGCCAA GCTCCAAGGA CTGAACCCAT GGCTGACTTG TCTTGAAGAC 
GGGCTCTGGT CCCTCCCGGA AGCCTACTGC AAGTTGGAGT GTGACGCCCC CCCTGTAATC 
CCGAATGCCA GCCTGCTCTT GCCTCACTGC CTCCAGGGCA ACCATGACGT GGGCTCCGTC 
TGTAGATACG AATGCAAACC AGGCTACTAT GCCGAGCCAA GTGCCGAGGG TAAAGTCAGG 
AGCAATTTCC TGAAGATACA GTGCCTGGAG GGTGGCATCT GGGAGCAAGG CAGCTGCGTT 
CCAGTGGTGT GTGAGCCCCC TTCCCCTGTC TTTGAAGGCA TGTATGAATG TACCAATGGC 
TTTGAACTCA ACAGCCGGTG TGTGCTCAGC TGTAACCAGG AAAGCGGAAG GGTTCCTATC 
CTCTGCACTA AGGAGGGACT GTGGACTGAG GAGTTCAAGT TGTGTGAGAA CCTGCAAGGG 
GAGTGCCCGC CACCCCCGTC GGAGCTGAAT TTTGTGGAGT ACAAGTGTGA ACAGGGATAC 
GGGATTGGTG CTGTGTGTTC CCCATCATGT GTGATTCCCC CTAGTGACCC CGTAGTTCTG 
CCTGAGAACG TCACTGCTGA CACGCTGGAG CACTGGATGG AACCTGTCAA AGTCCAGAGC 
ATTGTGTGCA CTGGACAGCG TCAATGGCAC CCACCCCCCT TCCTCATCCA CTGCATCCAG 
TCGTGTGAGC CTTTCCAAGC CGATGGGTGG TGCGATACCA TCAACAACCG GGCCTACTGC 
CACTATGATG GGGGAGACTG CTGCTCTTCT ACTCTGTCCT CCAAGAAGGT CATTCCATTT 
GCTGCTGACT GTGACCTGGA TGAATGCACC TGCCGGGACC CCAAGGCTGA AGAAAATCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003999270.1
CDS	1175..6517
Translation	MMGPKILRISLVILAGWALGTAGSEPGWTRKRSLAQRGRLNQVLSEGERCWLGARPPRPRAAPQHHLFGVYPSRPGDYLRPYPMGGQGTRPAGRSEPAATGAASPAGGVGLRGTAEQPAASWDRDGPAGRSELRGDDDTYLGDGGSKESLGEAGTRERSATAATAATTFVGPKEPKPETQSKGRAKTRRRRQVVKGQVGAVQGEPGVSPQRLWPRPKHPLVHGVRMSLSEDGIQKGGGDPSWEAESQGGLPVLYFSGRRGRLLLRPEVLAEIPREAFTVEAWVRPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDIGRRDARFFFSLRTDRVKKATIVTGNSRYQPGTWTHVAATYDGHRMALYVDGTQVASSPEQSGPLNSPFMASCRSLLLGGDSSEDGRYFRGHLGTLVFWSTALPQSHLRHSPQRPAGVEELASLILTASFEPLEEQWAPFRDGEFPRREVLHGFEPEPEIVSPLKPPLCGRTACDNVELISHYNGHRPLQGEKVIRYRVVNICDDEGLNPTVSEEQISRQHKALNHAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLHGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVADVHKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGAATWPWDKEAISHLGGVVLNPAYYGMPGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMDTGDLCADTAPTPKSKLCQDPEPTNDTCGFTHFPGAPFSNYMSYTDDDCTDSFTPNQVARMHCYLDLVYQQWSQSRKPTPIPIPPMVIGQTHTSLTIHWLPPISGVVHDRVPGSVCGACTEDGTFRQYVHAASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLYHMNMTVPCPSEGCSLELLFQHPVQADTLTLWVTYLSMDSSQALFDTEILLEHQESVHLGPLDTFCDTPLTIKLHVDGKVSGVKVYTFDERMEIDAALLTSQPHSPLCSGCRPVRYQVLREPPFANGLPMVVTNPHRKFTDVEVTPGQLYRYQVQAGAGTELGEASPPLSHIHGAPYCGDGKVAGSLGEECDDGDLLSGDGCSRACQLEEGFHCAGEPSLCYIYEGDGICEPFEKETSIIDCGLYTPKGYVDQWATQAYSSHEDKKCPASLVTGEPHSMICTSYHPDLPEHRPLTGWFPCVTNGNRPQDKRSEQTEGSLEREEEVWLKVCFSRPGVATTIFIFLTSDGPALGEHRRPTVTVHLTDVSGNNHSLGTYELSCQHNPLVINVSHHPNVLSHHTSSVLLNFSSPLVGISAVALRTSSHISSSTLNNCIPEHEGQNHQGQSCVHRPCGEQGSCAPLLLDHADMVNCTSGGPGHMKCAITCQRGFALQASSGQYLRPVQKEILLTCSSGHWDRAVSCMPLDCGVPDPSLVNYANFSCSEGTDFLKRCSISCVPPAKLQGLNPWLTCLEDGLWSLPEAYCKLECDAPPVIPNASLLLPHCLQGNHDVGSVCRYECKPGYYAEPSAEGKVRSNFLKIQCLEGGIWEQGSCVPVVCEPPSPVFEGMYECTNGFELNSRCVLSCNQESGRVPILCTKEGLWTEEFKLCENLQGECPPPPSELNFVEYKCEQGYGIGAVCSPSCVIPPSDPVVLPENVTADTLEHWMEPVKVQSIVCTGQRQWHPPPFLIHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_003999221.4
Organism	Felis catus(Domestic cat)
Definition	Felis catus pappalysin 2 (PAPPA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003999221.4

ORF Insert Sequence:

ATGATGGGCC CAAAGATCCT GAGAATAAGC CTGGTGATTC TGGCCGGGTG GGCGCTCGGC 
ACTGCCGGCT CTGAGCCGGG CTGGACACGC AAGAGGTCCC TGGCTCAGAG GGGACGCCTG 
AACCAGGTGC TGTCGGAAGG AGAACGCTGT TGGCTGGGGG CCAGGCCGCC AAGACCCAGA 
GCCGCTCCCC AGCATCATCT TTTTGGGGTC TACCCCAGCA GGCCTGGGGA CTACCTGAGG 
CCCTACCCCA TGGGGGGGCA GGGAACCCGC CCCGCGGGAC GCAGTGAGCC AGCTGCCACG 
GGGGCTGCGA GCCCAGCTGG AGGTGTGGGG CTGAGGGGCA CAGCTGAGCA GCCAGCCGCC 
TCGTGGGACA GGGATGGTCC CGCTGGGCGG TCTGAGCTGC GGGGAGACGA TGACACTTAT 
CTTGGTGATG GAGGATCCAA GGAGTCTCTA GGTGAGGCTG GGACTCGTGA ACGCTCGGCC 
ACGGCTGCCA CGGCTGCTAC CACCTTCGTG GGCCCAAAGG AACCCAAACC AGAGACCCAA 
AGCAAGGGCC GGGCCAAGAC CAGGCGTCGT CGCCAGGTGG TGAAGGGGCA GGTAGGGGCC 
GTGCAGGGAG AGCCCGGTGT CTCTCCCCAG CGTCTCTGGC CGCGGCCGAA GCATCCCCTT 
GTGCACGGGG TCAGAATGAG TCTGTCGGAG GACGGCATCC AGAAGGGTGG AGGGGACCCC 
TCCTGGGAAG CAGAGTCCCA GGGAGGACTG CCTGTCTTGT ATTTCTCCGG GAGGCGGGGG 
CGGCTCCTGC TGCGTCCAGA AGTGCTGGCT GAGATTCCCC GGGAGGCGTT CACAGTGGAA 
GCCTGGGTGA GACCGGAGGG AGGACAGAAC AACCCAGCCA TCATTGCAGG TGTGTTTGAT 
AACTGCTCCC ACACTGTCAG TGACAAGGGC TGGGCCCTAG GGATCCGCTC AGGGAAGGAC 
ATAGGAAGGC GAGACGCTCG CTTCTTCTTC TCCCTTCGCA CCGACCGTGT GAAGAAAGCC 
ACCATTGTGA CTGGCAACAG TCGCTACCAA CCAGGCACAT GGACACACGT GGCAGCCACC 
TATGATGGAC ATCGTATGGC TCTGTATGTG GATGGCACTC AGGTGGCCAG TAGCCCAGAG 
CAATCTGGTC CCCTGAACAG CCCCTTCATG GCATCCTGTC GCTCTTTGCT TCTGGGGGGG 
GATAGCTCTG AGGACGGCCG CTATTTTCGT GGACACCTGG GCACCCTGGT CTTCTGGTCG 
ACTGCCCTCC CGCAAAGCCA TCTCCGGCAC AGTCCTCAGC GTCCAGCTGG AGTGGAAGAA 
TTGGCCTCCC TCATCCTGAC AGCCAGTTTT GAGCCCTTGG AAGAACAGTG GGCCCCCTTT 
AGAGATGGGG AGTTCCCACG GCGTGAGGTT CTGCATGGCT TTGAGCCTGA GCCTGAGATT 
GTGTCACCTC TGAAGCCCCC ACTCTGTGGA CGGACGGCTT GTGACAACGT GGAACTGATC 
TCCCACTACA ACGGGCACCG GCCCCTCCAG GGAGAGAAGG TGATCCGCTA CCGAGTGGTA 
AACATCTGTG ACGATGAGGG CCTGAACCCC ACTGTGAGCG AGGAGCAGAT CAGCCGGCAG 
CACAAGGCGC TGAACCATGC CTTCAGCCGC TACAACATCA GCTGGCAGCT GAGCGTCCAC 
CAGGTCCACA ACTCCACCCT GCGCCACCGG GTTGTGCTTG TGAACTGCGA GCCCAGCAAG 
ATTGGCAATG ACCACTGTGA CCCTGAGTGT GAGCACCCAC TCACAGGCTA TGACGGGGGC 
GACTGCCGCC TACATGGCCG GTGCTACTCC TGGAACCGCA GGGACGGGCT CTGCCACGTG 
GAGTGCAACA ACATGCTGAA CGACTTTGAC GACGGGGACT GCTGCGACCC CGAGGTGGCC 
GATGTGCACA AGACCTGCTT TGACCCCGAC TCACCCAAGA GGGCATACAT GAGTGTGAAG 
GAGCTGAAGG AGGCACTGCA GCTCAACAGT ACTCACTTCC TCAATGTCTA CTTTGCCAGC 
TCCGTGCGGG AAGACCTTGC AGGCGCTGCC ACCTGGCCTT GGGACAAGGA AGCCATCAGT 
CACCTGGGTG GCGTTGTCCT CAACCCAGCC TATTATGGCA TGCCTGGCCA TACCAACATT 
ATGATCCACG AGGTAGGGCA TGTCCTGGGA CTCTACCATG TCTTCAAAGG GGTCAGTGAG 
AGGGAATCCT GTGATGACCC CTGTAGGGAG ACAGTGCCAT CCATGGATAC GGGAGACCTC 
TGTGCCGACA CCGCCCCTAC TCCCAAGAGT AAGCTGTGCC AGGACCCAGA GCCTACCAAC 
GATACCTGTG GCTTCACCCA CTTCCCGGGG GCTCCGTTCA GCAACTACAT GAGCTATACA 
GATGATGATT GCACCGACAG CTTCACCCCT AACCAGGTGG CCCGGATGCA TTGCTATTTG 
GACCTTGTGT ATCAGCAGTG GAGTCAAAGC CGGAAGCCCA CCCCCATTCC CATCCCACCC 
ATGGTCATCG GGCAGACCCA CACCTCCCTC ACCATCCACT GGCTTCCTCC AATCAGTGGG 
GTTGTACACG ACAGGGTCCC AGGCAGCGTG TGTGGTGCCT GCACTGAAGA TGGGACATTC 
CGTCAGTACG TGCACGCAGC TTCCTCCCGG CGTGTGTGTG ACTCCTCAGG TTACTGGACT 
CCAGAGGAGG CTGTGGGGCC TCCAGACGTG GACCAGCCCT GTGAGCCGAG CTTGCAGGCC 
TGGAGTCCCG AGCTCCACCT GTACCATATG AACATGACGG TCCCCTGCCC TTCGGAAGGC 
TGTAGCCTGG AGCTGCTCTT CCAGCACCCG GTCCAAGCTG ACACCCTTAC CCTATGGGTC 
ACCTACCTCT CCATGGACTC CTCCCAGGCA CTCTTTGACA CGGAGATCTT GCTGGAACAC 
CAGGAGTCTG TGCACCTGGG CCCCTTAGAC ACTTTCTGTG ACACCCCACT CACTATCAAA 
CTGCATGTGG ATGGGAAGGT CTCTGGGGTG AAGGTCTACA CCTTTGATGA GCGGATGGAG 
ATTGACGCAG CTCTGCTAAC TTCCCAGCCC CATAGTCCCC TGTGCTCTGG CTGCAGGCCT 
GTGAGGTACC AGGTTCTTCG TGAGCCCCCA TTTGCCAATG GCTTGCCCAT GGTGGTGACA 
AATCCTCACA GGAAGTTCAC GGACGTGGAG GTCACACCTG GGCAGCTGTA TCGGTACCAA 
GTTCAAGCTG GAGCTGGAAC AGAACTAGGA GAAGCCTCGC CTCCGCTGAG CCACATCCAT 
GGAGCTCCTT ACTGTGGAGA CGGGAAGGTG GCAGGGAGCC TGGGAGAAGA ATGTGATGAT 
GGGGACCTTC TGAGCGGAGA TGGCTGCTCA AGGGCGTGCC AGCTGGAGGA AGGCTTCCAC 
TGCGCAGGGG AGCCAAGCCT ATGTTACATA TATGAGGGAG ATGGGATATG TGAACCTTTT 
GAGAAGGAAA CCAGCATCAT AGACTGTGGC CTCTATACTC CCAAAGGATA TGTGGATCAG 
TGGGCCACCC AAGCTTATTC CTCTCATGAA GACAAGAAGT GTCCTGCTTC CTTGGTAACT 
GGAGAACCTC ATTCCATGAT TTGCACGTCA TACCATCCAG ACTTACCAGA GCACCGTCCT 
CTCACTGGCT GGTTTCCCTG TGTCACCAAT GGAAACAGAC CTCAGGATAA AAGGAGTGAG 
CAGACAGAAG GTAGCCTGGA GAGGGAGGAG GAGGTTTGGC TCAAAGTGTG TTTCAGTAGA 
CCAGGAGTGG CCACGACAAT TTTCATTTTC TTGACATCTG ATGGCCCGGC ACTTGGAGAA 
CATCGGCGGC CAACAGTGAC TGTTCACCTG ACTGATGTCA GTGGGAACAA CCACTCTCTT 
GGAACCTATG AACTGTCATG CCAGCATAAC CCGCTGGTTA TCAATGTGAG CCATCACCCG 
AATGTCCTCT CCCACCATAC CTCTTCAGTG CTGCTGAATT TCTCATCCCC ATTGGTGGGC 
ATCTCAGCGG TGGCTCTAAG GACATCCTCC CACATAAGTT CATCAACTCT CAATAACTGC 
ATCCCAGAGC ATGAGGGACA AAATCATCAG GGACAGAGCT GTGTCCACCG GCCCTGTGGG 
GAGCAGGGTA GCTGTGCGCC ATTGCTGCTT GATCATGCGG ATATGGTGAA CTGTACCTCT 
GGTGGCCCAG GTCACATGAA GTGTGCTATC ACCTGTCAAA GGGGGTTTGC CCTTCAGGCC 
AGCAGCGGGC AATACCTCAG GCCCGTGCAG AAGGAAATTC TGCTCACCTG TTCCTCTGGG 
CACTGGGACC GGGCTGTGAG CTGCATGCCC CTTGATTGTG GCGTTCCCGA CCCATCTTTG 
GTGAACTACG CAAACTTCTC CTGCTCAGAG GGAACAGACT TTCTGAAGCG CTGTTCAATC 
TCCTGTGTCC CACCAGCCAA GCTCCAAGGA CTGAACCCAT GGCTGACTTG TCTTGAAGAC 
GGGCTCTGGT CCCTCCCGGA AGCCTACTGC AAGTTGGAGT GTGACGCCCC CCCTGTAATC 
CCGAATGCCA GCCTGCTCTT GCCTCACTGC CTCCAGGGCA ACCATGACGT GGGCTCCGTC 
TGTAGATACG AATGCAAACC AGGCTACTAT GCCGAGCCAA GTGCCGAGGG TAAAGTCAGG 
AGCAATTTCC TGAAGATACA GTGCCTGGAG GGTGGCATCT GGGAGCAAGG CAGCTGCGTT 
CCAGTGGTGT GTGAGCCCCC TTCCCCTGTC TTTGAAGGCA TGTATGAATG TACCAATGGC 
TTTGAACTCA ACAGCCGGTG TGTGCTCAGC TGTAACCAGG AAAGCGGAAG GGTTCCTATC 
CTCTGCACTA AGGAGGGACT GTGGACTGAG GAGTTCAAGT TGTGTGAGAA CCTGCAAGGG 
GAGTGCCCGC CACCCCCGTC GGAGCTGAAT TTTGTGGAGT ACAAGTGTGA ACAGGGATAC 
GGGATTGGTG CTGTGTGTTC CCCATCATGT GTGATTCCCC CTAGTGACCC CGTAGTTCTG 
CCTGAGAACG TCACTGCTGA CACGCTGGAG CACTGGATGG AACCTGTCAA AGTCCAGAGC 
ATTGTGTGCA CTGGACAGCG TCAATGGCAC CCACCCCCCT TCCTCATCCA CTGCATCCAG 
TCGTGTGAGC CTTTCCAAGC CGATGGGTGG TGCGATACCA TCAACAACCG GGCCTACTGC 
CACTATGATG GGGGAGACTG CTGCTCTTCT ACTCTGTCCT CCAAGAAGGT CATTCCATTT 
GCTGCTGACT GTGACCTGGA TGAATGCACC TGCCGGGACC CCAAGGCTGA AGAAAATCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_019821662.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019677221.1 Felis catus pappalysin 2 (PAPPA2), transcript variant X3, mRNA.

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CloneID	OFb56298
Clone ID Related Accession (Same CDS sequence)	XM_019821662.1
Accession Version	XM_019821662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	pappalysin-2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGCC CAAAGATCCT GAGAATAAGC CTGGTGATTC TGGCCGGGTG GGCGCTCGGC 
ACTGCCGGCT CTGAGCCGGG CTGGACACGC AAGAGGTCCC TGGCTCAGAG GGGACGCCTG 
AACCAGGTGC TGTCGGAAGG AGAACGCTGT TGGCTGGGGG CCAGGCCGCC AAGACCCAGA 
GCCGCTCCCC AGCATCATCT TTTTGGGGTC TACCCCAGCA GGCCTGGGGA CTACCTGAGG 
CCCTACCCCA TGGGGGGGCA GGGAACCCGC CCCGCGGGAC GCAGTGAGCC AGCTGCCACG 
GGGGCTGCGA GCCCAGCTGG AGGTGTGGGG CTGAGGGGCA CAGCTGAGCA GCCAGCCGCC 
TCGTGGGACA GGGATGGTCC CGCTGGGCGG TCTGAGCTGC GGGGAGACGA TGACACTTAT 
CTTGGTGATG GAGGATCCAA GGAGTCTCTA GGTGAGGCTG GGACTCGTGA ACGCTCGGCC 
ACGGCTGCCA CGGCTGCTAC CACCTTCGTG GGCCCAAAGG AACCCAAACC AGAGACCCAA 
AGCAAGGGCC GGGCCAAGAC CAGGCGTCGT CGCCAGGTGG TGAAGGGGCA GGTAGGGGCC 
GTGCAGGGAG AGCCCGGTGT CTCTCCCCAG CGTCTCTGGC CGCGGCCGAA GCATCCCCTT 
GTGCACGGGG TCAGAATGAG TCTGTCGGAG GACGGCATCC AGAAGGGTGG AGGGGACCCC 
TCCTGGGAAG CAGAGTCCCA GGGAGGACTG CCTGTCTTGT ATTTCTCCGG GAGGCGGGGG 
CGGCTCCTGC TGCGTCCAGA AGTGCTGGCT GAGATTCCCC GGGAGGCGTT CACAGTGGAA 
GCCTGGGTGA GACCGGAGGG AGGACAGAAC AACCCAGCCA TCATTGCAGG TGTGTTTGAT 
AACTGCTCCC ACACTGTCAG TGACAAGGGC TGGGCCCTAG GGATCCGCTC AGGGAAGGAC 
ATAGGAAGGC GAGACGCTCG CTTCTTCTTC TCCCTTCGCA CCGACCGTGT GAAGAAAGCC 
ACCATTGTGA CTGGCAACAG TCGCTACCAA CCAGGCACAT GGACACACGT GGCAGCCACC 
TATGATGGAC ATCGTATGGC TCTGTATGTG GATGGCACTC AGGTGGCCAG TAGCCCAGAG 
CAATCTGGTC CCCTGAACAG CCCCTTCATG GCATCCTGTC GCTCTTTGCT TCTGGGGGGG 
GATAGCTCTG AGGACGGCCG CTATTTTCGT GGACACCTGG GCACCCTGGT CTTCTGGTCG 
ACTGCCCTCC CGCAAAGCCA TCTCCGGCAC AGTCCTCAGC GTCCAGCTGG AGTGGAAGAA 
TTGGCCTCCC TCATCCTGAC AGCCAGTTTT GAGCCCTTGG AAGAACAGTG GGCCCCCTTT 
AGAGATGGGG AGTTCCCACG GCGTGAGGTT CTGCATGGCT TTGAGCCTGA GCCTGAGATT 
GTGTCACCTC TGAAGCCCCC ACTCTGTGGA CGGACGGCTT GTGACAACGT GGAACTGATC 
TCCCACTACA ACGGGCACCG GCCCCTCCAG GGAGAGAAGG TGATCCGCTA CCGAGTGGTA 
AACATCTGTG ACGATGAGGG CCTGAACCCC ACTGTGAGCG AGGAGCAGAT CAGCCGGCAG 
CACAAGGCGC TGAACCATGC CTTCAGCCGC TACAACATCA GCTGGCAGCT GAGCGTCCAC 
CAGGTCCACA ACTCCACCCT GCGCCACCGG GTTGTGCTTG TGAACTGCGA GCCCAGCAAG 
ATTGGCAATG ACCACTGTGA CCCTGAGTGT GAGCACCCAC TCACAGGCTA TGACGGGGGC 
GACTGCCGCC TACATGGCCG GTGCTACTCC TGGAACCGCA GGGACGGGCT CTGCCACGTG 
GAGTGCAACA ACATGCTGAA CGACTTTGAC GACGGGGACT GCTGCGACCC CGAGGTGGCC 
GATGTGCACA AGACCTGCTT TGACCCCGAC TCACCCAAGA GCTCCGTGCG GGAAGACCTT 
GCAGGCGCTG CCACCTGGCC TTGGGACAAG GAAGCCATCA GTCACCTGGG TGGCGTTGTC 
CTCAACCCAG CCTATTATGG CATGCCTGGC CATACCAACA TTATGATCCA CGAGGTAGGG 
CATGTCCTGG GACTCTACCA TGTCTTCAAA GGGGTCAGTG AGAGGGAATC CTGTGATGAC 
CCCTGTAGGG AGACAGTGCC ATCCATGGAT ACGGGAGACC TCTGTGCCGA CACCGCCCCT 
ACTCCCAAGA GTAAGCTGTG CCAGGACCCA GAGCCTACCA ACGATACCTG TGGCTTCACC 
CACTTCCCGG GGGCTCCGTT CAGCAACTAC ATGAGCTATA CAGATGATGA TTGCACCGAC 
AGCTTCACCC CTAACCAGGT GGCCCGGATG CATTGCTATT TGGACCTTGT GTATCAGCAG 
TGGAGTCAAA GCCGGAAGCC CACCCCCATT CCCATCCCAC CCATGGTCAT CGGGCAGACC 
CACACCTCCC TCACCATCCA CTGGCTTCCT CCAATCAGTG GGGTTGTACA CGACAGGGTC 
CCAGGCAGCG TGTGTGGTGC CTGCACTGAA GATGGGACAT TCCGTCAGTA CGTGCACGCA 
GCTTCCTCCC GGCGTGTGTG TGACTCCTCA GGTTACTGGA CTCCAGAGGA GGCTGTGGGG 
CCTCCAGACG TGGACCAGCC CTGTGAGCCG AGCTTGCAGG CCTGGAGTCC CGAGCTCCAC 
CTGTACCATA TGAACATGAC GGTCCCCTGC CCTTCGGAAG GCTGTAGCCT GGAGCTGCTC 
TTCCAGCACC CGGTCCAAGC TGACACCCTT ACCCTATGGG TCACCTACCT CTCCATGGAC 
TCCTCCCAGG CACTCTTTGA CACGGAGATC TTGCTGGAAC ACCAGGAGTC TGTGCACCTG 
GGCCCCTTAG ACACTTTCTG TGACACCCCA CTCACTATCA AACTGCATGT GGATGGGAAG 
GTCTCTGGGG TGAAGGTCTA CACCTTTGAT GAGCGGATGG AGATTGACGC AGCTCTGCTA 
ACTTCCCAGC CCCATAGTCC CCTGTGCTCT GGCTGCAGGC CTGTGAGGTA CCAGGTTCTT 
CGTGAGCCCC CATTTGCCAA TGGCTTGCCC ATGGTGGTGA CAAATCCTCA CAGGAAGTTC 
ACGGACGTGG AGGTCACACC TGGGCAGCTG TATCGGTACC AAGTTCAAGC TGGAGCTGGA 
ACAGAACTAG GAGAAGCCTC GCCTCCGCTG AGCCACATCC ATGGAGCTCC TTACTGTGGA 
GACGGGAAGG TGGCAGGGAG CCTGGGAGAA GAATGTGATG ATGGGGACCT TCTGAGCGGA 
GATGGCTGCT CAAGGGCGTG CCAGCTGGAG GAAGGCTTCC ACTGCGCAGG GGAGCCAAGC 
CTATGTTACA TATATGAGGG AGATGGGATA TGTGAACCTT TTGAGAAGGA AACCAGCATC 
ATAGACTGTG GCCTCTATAC TCCCAAAGGA TATGTGGATC AGTGGGCCAC CCAAGCTTAT 
TCCTCTCATG AAGACAAGAA GTGTCCTGCT TCCTTGGTAA CTGGAGAACC TCATTCCATG 
ATTTGCACGT CATACCATCC AGACTTACCA GAGCACCGTC CTCTCACTGG CTGGTTTCCC 
TGTGTCACCA ATGGAAACAG ACCTCAGGAT AAAAGGAGTG AGCAGACAGA AGGTAGCCTG 
GAGAGGGAGG AGGAGGTTTG GCTCAAAGTG TGTTTCAGTA GACCAGGAGT GGCCACGACA 
ATTTTCATTT TCTTGACATC TGATGGCCCG GCACTTGGAG AACATCGGCG GCCAACAGTG 
ACTGTTCACC TGACTGATGT CAGTGGGAAC AACCACTCTC TTGGAACCTA TGAACTGTCA 
TGCCAGCATA ACCCGCTGGT TATCAATGTG AGCCATCACC CGAATGTCCT CTCCCACCAT 
ACCTCTTCAG TGCTGCTGAA TTTCTCATCC CCATTGGTGG GCATCTCAGC GGTGGCTCTA 
AGGACATCCT CCCACATAAG TTCATCAACT CTCAATAACT GCATCCCAGA GCATGAGGGA 
CAAAATCATC AGGGACAGAG CTGTGTCCAC CGGCCCTGTG GGGAGCAGGG TAGCTGTGCG 
CCATTGCTGC TTGATCATGC GGATATGGTG AACTGTACCT CTGGTGGCCC AGGTCACATG 
AAGTGTGCTA TCACCTGTCA AAGGGGGTTT GCCCTTCAGG CCAGCAGCGG GCAATACCTC 
AGGCCCGTGC AGAAGGAAAT TCTGCTCACC TGTTCCTCTG GGCACTGGGA CCGGGCTGTG 
AGCTGCATGC CCCTTGATTG TGGCGTTCCC GACCCATCTT TGGTGAACTA CGCAAACTTC 
TCCTGCTCAG AGGGAACAGA CTTTCTGAAG CGCTGTTCAA TCTCCTGTGT CCCACCAGCC 
AAGCTCCAAG GACTGAACCC ATGGCTGACT TGTCTTGAAG ACGGGCTCTG GTCCCTCCCG 
GAAGCCTACT GCAAGTTGGA GTGTGACGCC CCCCCTGTAA TCCCGAATGC CAGCCTGCTC 
TTGCCTCACT GCCTCCAGGG CAACCATGAC GTGGGCTCCG TCTGTAGATA CGAATGCAAA 
CCAGGCTACT ATGCCGAGCC AAGTGCCGAG GGTAAAGTCA GGAGCAATTT CCTGAAGATA 
CAGTGCCTGG AGGGTGGCAT CTGGGAGCAA GGCAGCTGCG TTCCAGTGGT GTGTGAGCCC 
CCTTCCCCTG TCTTTGAAGG CATGTATGAA TGTACCAATG GCTTTGAACT CAACAGCCGG 
TGTGTGCTCA GCTGTAACCA GGAAAGCGGA AGGGTTCCTA TCCTCTGCAC TAAGGAGGGA 
CTGTGGACTG AGGAGTTCAA GTTGTGTGAG AACCTGCAAG GGGAGTGCCC GCCACCCCCG 
TCGGAGCTGA ATTTTGTGGA GTACAAGTGT GAACAGGGAT ACGGGATTGG TGCTGTGTGT 
TCCCCATCAT GTGTGATTCC CCCTAGTGAC CCCGTAGTTC TGCCTGAGAA CGTCACTGCT 
GACACGCTGG AGCACTGGAT GGAACCTGTC AAAGTCCAGA GCATTGTGTG CACTGGACAG 
CGTCAATGGC ACCCACCCCC CTTCCTCATC CACTGCATCC AGTCGTGTGA GCCTTTCCAA 
GCCGATGGGT GGTGCGATAC CATCAACAAC CGGGCCTACT GCCACTATGA TGGGGGAGAC 
TGCTGCTCTT CTACTCTGTC CTCCAAGAAG GTCATTCCAT TTGCTGCTGA CTGTGACCTG 
GATGAATGCA CCTGCCGGGA CCCCAAGGCT GAAGAAAATC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019677221.1
CDS	1176..6440
Translation	MMGPKILRISLVILAGWALGTAGSEPGWTRKRSLAQRGRLNQVLSEGERCWLGARPPRPRAAPQHHLFGVYPSRPGDYLRPYPMGGQGTRPAGRSEPAATGAASPAGGVGLRGTAEQPAASWDRDGPAGRSELRGDDDTYLGDGGSKESLGEAGTRERSATAATAATTFVGPKEPKPETQSKGRAKTRRRRQVVKGQVGAVQGEPGVSPQRLWPRPKHPLVHGVRMSLSEDGIQKGGGDPSWEAESQGGLPVLYFSGRRGRLLLRPEVLAEIPREAFTVEAWVRPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDIGRRDARFFFSLRTDRVKKATIVTGNSRYQPGTWTHVAATYDGHRMALYVDGTQVASSPEQSGPLNSPFMASCRSLLLGGDSSEDGRYFRGHLGTLVFWSTALPQSHLRHSPQRPAGVEELASLILTASFEPLEEQWAPFRDGEFPRREVLHGFEPEPEIVSPLKPPLCGRTACDNVELISHYNGHRPLQGEKVIRYRVVNICDDEGLNPTVSEEQISRQHKALNHAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLHGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVADVHKTCFDPDSPKSSVREDLAGAATWPWDKEAISHLGGVVLNPAYYGMPGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMDTGDLCADTAPTPKSKLCQDPEPTNDTCGFTHFPGAPFSNYMSYTDDDCTDSFTPNQVARMHCYLDLVYQQWSQSRKPTPIPIPPMVIGQTHTSLTIHWLPPISGVVHDRVPGSVCGACTEDGTFRQYVHAASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLYHMNMTVPCPSEGCSLELLFQHPVQADTLTLWVTYLSMDSSQALFDTEILLEHQESVHLGPLDTFCDTPLTIKLHVDGKVSGVKVYTFDERMEIDAALLTSQPHSPLCSGCRPVRYQVLREPPFANGLPMVVTNPHRKFTDVEVTPGQLYRYQVQAGAGTELGEASPPLSHIHGAPYCGDGKVAGSLGEECDDGDLLSGDGCSRACQLEEGFHCAGEPSLCYIYEGDGICEPFEKETSIIDCGLYTPKGYVDQWATQAYSSHEDKKCPASLVTGEPHSMICTSYHPDLPEHRPLTGWFPCVTNGNRPQDKRSEQTEGSLEREEEVWLKVCFSRPGVATTIFIFLTSDGPALGEHRRPTVTVHLTDVSGNNHSLGTYELSCQHNPLVINVSHHPNVLSHHTSSVLLNFSSPLVGISAVALRTSSHISSSTLNNCIPEHEGQNHQGQSCVHRPCGEQGSCAPLLLDHADMVNCTSGGPGHMKCAITCQRGFALQASSGQYLRPVQKEILLTCSSGHWDRAVSCMPLDCGVPDPSLVNYANFSCSEGTDFLKRCSISCVPPAKLQGLNPWLTCLEDGLWSLPEAYCKLECDAPPVIPNASLLLPHCLQGNHDVGSVCRYECKPGYYAEPSAEGKVRSNFLKIQCLEGGIWEQGSCVPVVCEPPSPVFEGMYECTNGFELNSRCVLSCNQESGRVPILCTKEGLWTEEFKLCENLQGECPPPPSELNFVEYKCEQGYGIGAVCSPSCVIPPSDPVVLPENVTADTLEHWMEPVKVQSIVCTGQRQWHPPPFLIHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_019821662.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus pappalysin 2 (PAPPA2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019821662.1

ORF Insert Sequence:

ATGATGGGCC CAAAGATCCT GAGAATAAGC CTGGTGATTC TGGCCGGGTG GGCGCTCGGC 
ACTGCCGGCT CTGAGCCGGG CTGGACACGC AAGAGGTCCC TGGCTCAGAG GGGACGCCTG 
AACCAGGTGC TGTCGGAAGG AGAACGCTGT TGGCTGGGGG CCAGGCCGCC AAGACCCAGA 
GCCGCTCCCC AGCATCATCT TTTTGGGGTC TACCCCAGCA GGCCTGGGGA CTACCTGAGG 
CCCTACCCCA TGGGGGGGCA GGGAACCCGC CCCGCGGGAC GCAGTGAGCC AGCTGCCACG 
GGGGCTGCGA GCCCAGCTGG AGGTGTGGGG CTGAGGGGCA CAGCTGAGCA GCCAGCCGCC 
TCGTGGGACA GGGATGGTCC CGCTGGGCGG TCTGAGCTGC GGGGAGACGA TGACACTTAT 
CTTGGTGATG GAGGATCCAA GGAGTCTCTA GGTGAGGCTG GGACTCGTGA ACGCTCGGCC 
ACGGCTGCCA CGGCTGCTAC CACCTTCGTG GGCCCAAAGG AACCCAAACC AGAGACCCAA 
AGCAAGGGCC GGGCCAAGAC CAGGCGTCGT CGCCAGGTGG TGAAGGGGCA GGTAGGGGCC 
GTGCAGGGAG AGCCCGGTGT CTCTCCCCAG CGTCTCTGGC CGCGGCCGAA GCATCCCCTT 
GTGCACGGGG TCAGAATGAG TCTGTCGGAG GACGGCATCC AGAAGGGTGG AGGGGACCCC 
TCCTGGGAAG CAGAGTCCCA GGGAGGACTG CCTGTCTTGT ATTTCTCCGG GAGGCGGGGG 
CGGCTCCTGC TGCGTCCAGA AGTGCTGGCT GAGATTCCCC GGGAGGCGTT CACAGTGGAA 
GCCTGGGTGA GACCGGAGGG AGGACAGAAC AACCCAGCCA TCATTGCAGG TGTGTTTGAT 
AACTGCTCCC ACACTGTCAG TGACAAGGGC TGGGCCCTAG GGATCCGCTC AGGGAAGGAC 
ATAGGAAGGC GAGACGCTCG CTTCTTCTTC TCCCTTCGCA CCGACCGTGT GAAGAAAGCC 
ACCATTGTGA CTGGCAACAG TCGCTACCAA CCAGGCACAT GGACACACGT GGCAGCCACC 
TATGATGGAC ATCGTATGGC TCTGTATGTG GATGGCACTC AGGTGGCCAG TAGCCCAGAG 
CAATCTGGTC CCCTGAACAG CCCCTTCATG GCATCCTGTC GCTCTTTGCT TCTGGGGGGG 
GATAGCTCTG AGGACGGCCG CTATTTTCGT GGACACCTGG GCACCCTGGT CTTCTGGTCG 
ACTGCCCTCC CGCAAAGCCA TCTCCGGCAC AGTCCTCAGC GTCCAGCTGG AGTGGAAGAA 
TTGGCCTCCC TCATCCTGAC AGCCAGTTTT GAGCCCTTGG AAGAACAGTG GGCCCCCTTT 
AGAGATGGGG AGTTCCCACG GCGTGAGGTT CTGCATGGCT TTGAGCCTGA GCCTGAGATT 
GTGTCACCTC TGAAGCCCCC ACTCTGTGGA CGGACGGCTT GTGACAACGT GGAACTGATC 
TCCCACTACA ACGGGCACCG GCCCCTCCAG GGAGAGAAGG TGATCCGCTA CCGAGTGGTA 
AACATCTGTG ACGATGAGGG CCTGAACCCC ACTGTGAGCG AGGAGCAGAT CAGCCGGCAG 
CACAAGGCGC TGAACCATGC CTTCAGCCGC TACAACATCA GCTGGCAGCT GAGCGTCCAC 
CAGGTCCACA ACTCCACCCT GCGCCACCGG GTTGTGCTTG TGAACTGCGA GCCCAGCAAG 
ATTGGCAATG ACCACTGTGA CCCTGAGTGT GAGCACCCAC TCACAGGCTA TGACGGGGGC 
GACTGCCGCC TACATGGCCG GTGCTACTCC TGGAACCGCA GGGACGGGCT CTGCCACGTG 
GAGTGCAACA ACATGCTGAA CGACTTTGAC GACGGGGACT GCTGCGACCC CGAGGTGGCC 
GATGTGCACA AGACCTGCTT TGACCCCGAC TCACCCAAGA GCTCCGTGCG GGAAGACCTT 
GCAGGCGCTG CCACCTGGCC TTGGGACAAG GAAGCCATCA GTCACCTGGG TGGCGTTGTC 
CTCAACCCAG CCTATTATGG CATGCCTGGC CATACCAACA TTATGATCCA CGAGGTAGGG 
CATGTCCTGG GACTCTACCA TGTCTTCAAA GGGGTCAGTG AGAGGGAATC CTGTGATGAC 
CCCTGTAGGG AGACAGTGCC ATCCATGGAT ACGGGAGACC TCTGTGCCGA CACCGCCCCT 
ACTCCCAAGA GTAAGCTGTG CCAGGACCCA GAGCCTACCA ACGATACCTG TGGCTTCACC 
CACTTCCCGG GGGCTCCGTT CAGCAACTAC ATGAGCTATA CAGATGATGA TTGCACCGAC 
AGCTTCACCC CTAACCAGGT GGCCCGGATG CATTGCTATT TGGACCTTGT GTATCAGCAG 
TGGAGTCAAA GCCGGAAGCC CACCCCCATT CCCATCCCAC CCATGGTCAT CGGGCAGACC 
CACACCTCCC TCACCATCCA CTGGCTTCCT CCAATCAGTG GGGTTGTACA CGACAGGGTC 
CCAGGCAGCG TGTGTGGTGC CTGCACTGAA GATGGGACAT TCCGTCAGTA CGTGCACGCA 
GCTTCCTCCC GGCGTGTGTG TGACTCCTCA GGTTACTGGA CTCCAGAGGA GGCTGTGGGG 
CCTCCAGACG TGGACCAGCC CTGTGAGCCG AGCTTGCAGG CCTGGAGTCC CGAGCTCCAC 
CTGTACCATA TGAACATGAC GGTCCCCTGC CCTTCGGAAG GCTGTAGCCT GGAGCTGCTC 
TTCCAGCACC CGGTCCAAGC TGACACCCTT ACCCTATGGG TCACCTACCT CTCCATGGAC 
TCCTCCCAGG CACTCTTTGA CACGGAGATC TTGCTGGAAC ACCAGGAGTC TGTGCACCTG 
GGCCCCTTAG ACACTTTCTG TGACACCCCA CTCACTATCA AACTGCATGT GGATGGGAAG 
GTCTCTGGGG TGAAGGTCTA CACCTTTGAT GAGCGGATGG AGATTGACGC AGCTCTGCTA 
ACTTCCCAGC CCCATAGTCC CCTGTGCTCT GGCTGCAGGC CTGTGAGGTA CCAGGTTCTT 
CGTGAGCCCC CATTTGCCAA TGGCTTGCCC ATGGTGGTGA CAAATCCTCA CAGGAAGTTC 
ACGGACGTGG AGGTCACACC TGGGCAGCTG TATCGGTACC AAGTTCAAGC TGGAGCTGGA 
ACAGAACTAG GAGAAGCCTC GCCTCCGCTG AGCCACATCC ATGGAGCTCC TTACTGTGGA 
GACGGGAAGG TGGCAGGGAG CCTGGGAGAA GAATGTGATG ATGGGGACCT TCTGAGCGGA 
GATGGCTGCT CAAGGGCGTG CCAGCTGGAG GAAGGCTTCC ACTGCGCAGG GGAGCCAAGC 
CTATGTTACA TATATGAGGG AGATGGGATA TGTGAACCTT TTGAGAAGGA AACCAGCATC 
ATAGACTGTG GCCTCTATAC TCCCAAAGGA TATGTGGATC AGTGGGCCAC CCAAGCTTAT 
TCCTCTCATG AAGACAAGAA GTGTCCTGCT TCCTTGGTAA CTGGAGAACC TCATTCCATG 
ATTTGCACGT CATACCATCC AGACTTACCA GAGCACCGTC CTCTCACTGG CTGGTTTCCC 
TGTGTCACCA ATGGAAACAG ACCTCAGGAT AAAAGGAGTG AGCAGACAGA AGGTAGCCTG 
GAGAGGGAGG AGGAGGTTTG GCTCAAAGTG TGTTTCAGTA GACCAGGAGT GGCCACGACA 
ATTTTCATTT TCTTGACATC TGATGGCCCG GCACTTGGAG AACATCGGCG GCCAACAGTG 
ACTGTTCACC TGACTGATGT CAGTGGGAAC AACCACTCTC TTGGAACCTA TGAACTGTCA 
TGCCAGCATA ACCCGCTGGT TATCAATGTG AGCCATCACC CGAATGTCCT CTCCCACCAT 
ACCTCTTCAG TGCTGCTGAA TTTCTCATCC CCATTGGTGG GCATCTCAGC GGTGGCTCTA 
AGGACATCCT CCCACATAAG TTCATCAACT CTCAATAACT GCATCCCAGA GCATGAGGGA 
CAAAATCATC AGGGACAGAG CTGTGTCCAC CGGCCCTGTG GGGAGCAGGG TAGCTGTGCG 
CCATTGCTGC TTGATCATGC GGATATGGTG AACTGTACCT CTGGTGGCCC AGGTCACATG 
AAGTGTGCTA TCACCTGTCA AAGGGGGTTT GCCCTTCAGG CCAGCAGCGG GCAATACCTC 
AGGCCCGTGC AGAAGGAAAT TCTGCTCACC TGTTCCTCTG GGCACTGGGA CCGGGCTGTG 
AGCTGCATGC CCCTTGATTG TGGCGTTCCC GACCCATCTT TGGTGAACTA CGCAAACTTC 
TCCTGCTCAG AGGGAACAGA CTTTCTGAAG CGCTGTTCAA TCTCCTGTGT CCCACCAGCC 
AAGCTCCAAG GACTGAACCC ATGGCTGACT TGTCTTGAAG ACGGGCTCTG GTCCCTCCCG 
GAAGCCTACT GCAAGTTGGA GTGTGACGCC CCCCCTGTAA TCCCGAATGC CAGCCTGCTC 
TTGCCTCACT GCCTCCAGGG CAACCATGAC GTGGGCTCCG TCTGTAGATA CGAATGCAAA 
CCAGGCTACT ATGCCGAGCC AAGTGCCGAG GGTAAAGTCA GGAGCAATTT CCTGAAGATA 
CAGTGCCTGG AGGGTGGCAT CTGGGAGCAA GGCAGCTGCG TTCCAGTGGT GTGTGAGCCC 
CCTTCCCCTG TCTTTGAAGG CATGTATGAA TGTACCAATG GCTTTGAACT CAACAGCCGG 
TGTGTGCTCA GCTGTAACCA GGAAAGCGGA AGGGTTCCTA TCCTCTGCAC TAAGGAGGGA 
CTGTGGACTG AGGAGTTCAA GTTGTGTGAG AACCTGCAAG GGGAGTGCCC GCCACCCCCG 
TCGGAGCTGA ATTTTGTGGA GTACAAGTGT GAACAGGGAT ACGGGATTGG TGCTGTGTGT 
TCCCCATCAT GTGTGATTCC CCCTAGTGAC CCCGTAGTTC TGCCTGAGAA CGTCACTGCT 
GACACGCTGG AGCACTGGAT GGAACCTGTC AAAGTCCAGA GCATTGTGTG CACTGGACAG 
CGTCAATGGC ACCCACCCCC CTTCCTCATC CACTGCATCC AGTCGTGTGA GCCTTTCCAA 
GCCGATGGGT GGTGCGATAC CATCAACAAC CGGGCCTACT GCCACTATGA TGGGGGAGAC 
TGCTGCTCTT CTACTCTGTC CTCCAAGAAG GTCATTCCAT TTGCTGCTGA CTGTGACCTG 
GATGAATGCA CCTGCCGGGA CCCCAAGGCT GAAGAAAATC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_019821663.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019677222.1 Felis catus pappalysin 2 (PAPPA2), transcript variant X4, mRNA.

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CloneID	OFb56299
Clone ID Related Accession (Same CDS sequence)	XM_019821663.1
Accession Version	XM_019821663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	pappalysin-2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGCC CAAAGATCCT GAGAATAAGC CTGGTGATTC TGGCCGGGTG GGCGCTCGGC 
ACTGCCGGCT CTGAGCCGGG CTGGACACGC AAGAGGTCCC TGGCTCAGAG GGGACGCCTG 
AACCAGGTGC TGTCGGAAGG AGAACGCTGT TGGCTGGGGG CCAGGCCGCC AAGACCCAGA 
GCCGCTCCCC AGCATCATCT TTTTGGGGTC TACCCCAGCA GGCCTGGGGA CTACCTGAGG 
CCCTACCCCA TGGGGGGGCA GGGAACCCGC CCCGCGGGAC GCAGTGAGCC AGCTGCCACG 
GGGGCTGCGA GCCCAGCTGG AGGTGTGGGG CTGAGGGGCA CAGCTGAGCA GCCAGCCGCC 
TCGTGGGACA GGGATGGTCC CGCTGGGCGG TCTGAGCTGC GGGGAGACGA TGACACTTAT 
CTTGGTGATG GAGGATCCAA GGAGTCTCTA GGTGAGGCTG GGACTCGTGA ACGCTCGGCC 
ACGGCTGCCA CGGCTGCTAC CACCTTCGTG GGCCCAAAGG AACCCAAACC AGAGACCCAA 
AGCAAGGGCC GGGCCAAGAC CAGGCGTCGT CGCCAGGTGG TGAAGGGGCA GGTAGGGGCC 
GTGCAGGGAG AGCCCGGTGT CTCTCCCCAG CGTCTCTGGC CGCGGCCGAA GCATCCCCTT 
GTGCACGGGG TCAGAATGAG TCTGTCGGAG GACGGCATCC AGAAGGGTGG AGGGGACCCC 
TCCTGGGAAG CAGAGTCCCA GGGAGGACTG CCTGTCTTGT ATTTCTCCGG GAGGCGGGGG 
CGGCTCCTGC TGCGTCCAGA AGTGCTGGCT GAGATTCCCC GGGAGGCGTT CACAGTGGAA 
GCCTGGGTGA GACCGGAGGG AGGACAGAAC AACCCAGCCA TCATTGCAGG TGTGTTTGAT 
AACTGCTCCC ACACTGTCAG TGACAAGGGC TGGGCCCTAG GGATCCGCTC AGGGAAGGAC 
ATAGGAAGGC GAGACGCTCG CTTCTTCTTC TCCCTTCGCA CCGACCGTGT GAAGAAAGCC 
ACCATTGTGA CTGGCAACAG TCGCTACCAA CCAGGCACAT GGACACACGT GGCAGCCACC 
TATGATGGAC ATCGTATGGC TCTGTATGTG GATGGCACTC AGGTGGCCAG TAGCCCAGAG 
CAATCTGGTC CCCTGAACAG CCCCTTCATG GCATCCTGTC GCTCTTTGCT TCTGGGGGGG 
GATAGCTCTG AGGACGGCCG CTATTTTCGT GGACACCTGG GCACCCTGGT CTTCTGGTCG 
ACTGCCCTCC CGCAAAGCCA TCTCCGGCAC AGTCCTCAGC GTCCAGCTGG AGTGGAAGAA 
TTGGCCTCCC TCATCCTGAC AGCCAGTTTT GAGCCCTTGG AAGAACAGTG GGCCCCCTTT 
AGAGATGGGG AGTTCCCACG GCGTGAGGTT CTGCATGGCT TTGAGCCTGA GCCTGAGATT 
GTGTCACCTC TGAAGCCCCC ACTCTGTGGA CGGACGGCTT GTGACAACGT GGAACTGATC 
TCCCACTACA ACGGGCACCG GCCCCTCCAG GGAGAGAAGG TGATCCGCTA CCGAGTGGTA 
AACATCTGTG ACGATGAGGG CCTGAACCCC ACTGTGAGCG AGGAGCAGAT CAGCCGGCAG 
CACAAGGCGC TGAACCATGC CTTCAGCCGC TACAACATCA GCTGGCAGCT GAGCGTCCAC 
CAGGTCCACA ACTCCACCCT GCGCCACCGG GTTGTGCTTG TGAACTGCGA GCCCAGCAAG 
ATTGGCAATG ACCACTGTGA CCCTGAGTGT GAGCACCCAC TCACAGGCTA TGACGGGGGC 
GACTGCCGCC TACATGGCCG GTGCTACTCC TGGAACCGCA GGGACGGGCT CTGCCACGTG 
GAGTGCAACA ACATGCTGAA CGACTTTGAC GACGGGGACT GCTGCGACCC CGAGGTGGCC 
GATGTGCACA AGACCTGCTT TGACCCCGAC TCACCCAAGA GGGCATACAT GAGTGTGAAG 
GAGCTGAAGG AGGCACTGCA GCTCAACAGT ACTCACTTCC TCAATGTCTA CTTTGCCAGC 
TCCGTGCGGG AAGACCTTGC AGGCGCTGCC ACCTGGCCTT GGGACAAGGA AGCCATCAGT 
CACCTGGGTG GCGTTGTCCT CAACCCAGCC TATTATGGCA TGCCTGGCCA TACCAACATT 
ATGATCCACG AGGTAGGGCA TGTCCTGGGA CTCTACCATG TCTTCAAAGG GGTCAGTGAG 
AGGGAATCCT GTGATGACCC CTGTAGGGAG ACAGTGCCAT CCATGGATAC GGGAGACCTC 
TGTGCCGACA CCGCCCCTAC TCCCAAGAGT AAGCTGTGCC AGGACCCAGA GCCTACCAAC 
GATACCTGTG GCTTCACCCA CTTCCCGGGG GCTCCGTTCA GCAACTACAT GAGCTATACA 
GATGATGATT GCACCGACAG CTTCACCCCT AACCAGGTGG CCCGGATGCA TTGCTATTTG 
GACCTTGTGT ATCAGCAGTG GAGTCAAAGC CGGAAGCCCA CCCCCATTCC CATCCCACCC 
ATGGTCATCG GGCAGACCCA CACCTCCCTC ACCATCCACT GGCTTCCTCC AATCAGTGGG 
GTTGTACACG ACAGGGTCCC AGGCAGCGTG TGTGGTGCCT GCACTGAAGA TGGGACATTC 
CGTCAGTACG TGCACGCAGC TTCCTCCCGG CGTGTGTGTG ACTCCTCAGG TTACTGGACT 
CCAGAGGAGG CTGTGGGGCC TCCAGACGTG GACCAGCCCT GTGAGCCGAG CTTGCAGGCC 
TGGAGTCCCG AGCTCCACCT GTACCATATG AACATGACGG TCCCCTGCCC TTCGGAAGGC 
TGTAGCCTGG AGCTGCTCTT CCAGCACCCG GTCCAAGCTG ACACCCTTAC CCTATGGGTC 
ACCTACCTCT CCATGGACTC CTCCCAGGCA CTCTTTGACA CGGAGATCTT GCTGGAACAC 
CAGGAGTCTG TGCACCTGGG CCCCTTAGAC ACTTTCTGTG ACACCCCACT CACTATCAAA 
CTGCATGTGG ATGGGAAGGT CTCTGGGGTG AAGGTCTACA CCTTTGATGA GCGGATGGAG 
ATTGACGCAG CTCTGCTAAC TTCCCAGCCC CATAGTCCCC TGTGCTCTGG CTGCAGGCCT 
GTGAGGGAGG TCACACCTGG GCAGCTGTAT CGGTACCAAG TTCAAGCTGG AGCTGGAACA 
GAACTAGGAG AAGCCTCGCC TCCGCTGAGC CACATCCATG GAGCTCCTTA CTGTGGAGAC 
GGGAAGGTGG CAGGGAGCCT GGGAGAAGAA TGTGATGATG GGGACCTTCT GAGCGGAGAT 
GGCTGCTCAA GGGCGTGCCA GCTGGAGGAA GGCTTCCACT GCGCAGGGGA GCCAAGCCTA 
TGTTACATAT ATGAGGGAGA TGGGATATGT GAACCTTTTG AGAAGGAAAC CAGCATCATA 
GACTGTGGCC TCTATACTCC CAAAGGATAT GTGGATCAGT GGGCCACCCA AGCTTATTCC 
TCTCATGAAG ACAAGAAGTG TCCTGCTTCC TTGGTAACTG GAGAACCTCA TTCCATGATT 
TGCACGTCAT ACCATCCAGA CTTACCAGAG CACCGTCCTC TCACTGGCTG GTTTCCCTGT 
GTCACCAATG GAAACAGACC TCAGGATAAA AGGAGTGAGC AGACAGAAGG TAGCCTGGAG 
AGGGAGGAGG AGGTTTGGCT CAAAGTGTGT TTCAGTAGAC CAGGAGTGGC CACGACAATT 
TTCATTTTCT TGACATCTGA TGGCCCGGCA CTTGGAGAAC ATCGGCGGCC AACAGTGACT 
GTTCACCTGA CTGATGTCAG TGGGAACAAC CACTCTCTTG GAACCTATGA ACTGTCATGC 
CAGCATAACC CGCTGGTTAT CAATGTGAGC CATCACCCGA ATGTCCTCTC CCACCATACC 
TCTTCAGTGC TGCTGAATTT CTCATCCCCA TTGGTGGGCA TCTCAGCGGT GGCTCTAAGG 
ACATCCTCCC ACATAAGTTC ATCAACTCTC AATAACTGCA TCCCAGAGCA TGAGGGACAA 
AATCATCAGG GACAGAGCTG TGTCCACCGG CCCTGTGGGG AGCAGGGTAG CTGTGCGCCA 
TTGCTGCTTG ATCATGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCAGG TCACATGAAG 
TGTGCTATCA CCTGTCAAAG GGGGTTTGCC CTTCAGGCCA GCAGCGGGCA ATACCTCAGG 
CCCGTGCAGA AGGAAATTCT GCTCACCTGT TCCTCTGGGC ACTGGGACCG GGCTGTGAGC 
TGCATGCCCC TTGATTGTGG CGTTCCCGAC CCATCTTTGG TGAACTACGC AAACTTCTCC 
TGCTCAGAGG GAACAGACTT TCTGAAGCGC TGTTCAATCT CCTGTGTCCC ACCAGCCAAG 
CTCCAAGGAC TGAACCCATG GCTGACTTGT CTTGAAGACG GGCTCTGGTC CCTCCCGGAA 
GCCTACTGCA AGTTGGAGTG TGACGCCCCC CCTGTAATCC CGAATGCCAG CCTGCTCTTG 
CCTCACTGCC TCCAGGGCAA CCATGACGTG GGCTCCGTCT GTAGATACGA ATGCAAACCA 
GGCTACTATG CCGAGCCAAG TGCCGAGGGT AAAGTCAGGA GCAATTTCCT GAAGATACAG 
TGCCTGGAGG GTGGCATCTG GGAGCAAGGC AGCTGCGTTC CAGTGGTGTG TGAGCCCCCT 
TCCCCTGTCT TTGAAGGCAT GTATGAATGT ACCAATGGCT TTGAACTCAA CAGCCGGTGT 
GTGCTCAGCT GTAACCAGGA AAGCGGAAGG GTTCCTATCC TCTGCACTAA GGAGGGACTG 
TGGACTGAGG AGTTCAAGTT GTGTGAGAAC CTGCAAGGGG AGTGCCCGCC ACCCCCGTCG 
GAGCTGAATT TTGTGGAGTA CAAGTGTGAA CAGGGATACG GGATTGGTGC TGTGTGTTCC 
CCATCATGTG TGATTCCCCC TAGTGACCCC GTAGTTCTGC CTGAGAACGT CACTGCTGAC 
ACGCTGGAGC ACTGGATGGA ACCTGTCAAA GTCCAGAGCA TTGTGTGCAC TGGACAGCGT 
CAATGGCACC CACCCCCCTT CCTCATCCAC TGCATCCAGT CGTGTGAGCC TTTCCAAGCC 
GATGGGTGGT GCGATACCAT CAACAACCGG GCCTACTGCC ACTATGATGG GGGAGACTGC 
TGCTCTTCTA CTCTGTCCTC CAAGAAGGTC ATTCCATTTG CTGCTGACTG TGACCTGGAT 
GAATGCACCT GCCGGGACCC CAAGGCTGAA GAAAATCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019677222.1
CDS	1176..6437
Translation	MMGPKILRISLVILAGWALGTAGSEPGWTRKRSLAQRGRLNQVLSEGERCWLGARPPRPRAAPQHHLFGVYPSRPGDYLRPYPMGGQGTRPAGRSEPAATGAASPAGGVGLRGTAEQPAASWDRDGPAGRSELRGDDDTYLGDGGSKESLGEAGTRERSATAATAATTFVGPKEPKPETQSKGRAKTRRRRQVVKGQVGAVQGEPGVSPQRLWPRPKHPLVHGVRMSLSEDGIQKGGGDPSWEAESQGGLPVLYFSGRRGRLLLRPEVLAEIPREAFTVEAWVRPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDIGRRDARFFFSLRTDRVKKATIVTGNSRYQPGTWTHVAATYDGHRMALYVDGTQVASSPEQSGPLNSPFMASCRSLLLGGDSSEDGRYFRGHLGTLVFWSTALPQSHLRHSPQRPAGVEELASLILTASFEPLEEQWAPFRDGEFPRREVLHGFEPEPEIVSPLKPPLCGRTACDNVELISHYNGHRPLQGEKVIRYRVVNICDDEGLNPTVSEEQISRQHKALNHAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLHGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVADVHKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGAATWPWDKEAISHLGGVVLNPAYYGMPGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMDTGDLCADTAPTPKSKLCQDPEPTNDTCGFTHFPGAPFSNYMSYTDDDCTDSFTPNQVARMHCYLDLVYQQWSQSRKPTPIPIPPMVIGQTHTSLTIHWLPPISGVVHDRVPGSVCGACTEDGTFRQYVHAASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLYHMNMTVPCPSEGCSLELLFQHPVQADTLTLWVTYLSMDSSQALFDTEILLEHQESVHLGPLDTFCDTPLTIKLHVDGKVSGVKVYTFDERMEIDAALLTSQPHSPLCSGCRPVREVTPGQLYRYQVQAGAGTELGEASPPLSHIHGAPYCGDGKVAGSLGEECDDGDLLSGDGCSRACQLEEGFHCAGEPSLCYIYEGDGICEPFEKETSIIDCGLYTPKGYVDQWATQAYSSHEDKKCPASLVTGEPHSMICTSYHPDLPEHRPLTGWFPCVTNGNRPQDKRSEQTEGSLEREEEVWLKVCFSRPGVATTIFIFLTSDGPALGEHRRPTVTVHLTDVSGNNHSLGTYELSCQHNPLVINVSHHPNVLSHHTSSVLLNFSSPLVGISAVALRTSSHISSSTLNNCIPEHEGQNHQGQSCVHRPCGEQGSCAPLLLDHADMVNCTSGGPGHMKCAITCQRGFALQASSGQYLRPVQKEILLTCSSGHWDRAVSCMPLDCGVPDPSLVNYANFSCSEGTDFLKRCSISCVPPAKLQGLNPWLTCLEDGLWSLPEAYCKLECDAPPVIPNASLLLPHCLQGNHDVGSVCRYECKPGYYAEPSAEGKVRSNFLKIQCLEGGIWEQGSCVPVVCEPPSPVFEGMYECTNGFELNSRCVLSCNQESGRVPILCTKEGLWTEEFKLCENLQGECPPPPSELNFVEYKCEQGYGIGAVCSPSCVIPPSDPVVLPENVTADTLEHWMEPVKVQSIVCTGQRQWHPPPFLIHCIQSCEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ

Target ORF information:

RefSeq Version	XM_019821663.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus pappalysin 2 (PAPPA2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019821663.1

ORF Insert Sequence:

ATGATGGGCC CAAAGATCCT GAGAATAAGC CTGGTGATTC TGGCCGGGTG GGCGCTCGGC 
ACTGCCGGCT CTGAGCCGGG CTGGACACGC AAGAGGTCCC TGGCTCAGAG GGGACGCCTG 
AACCAGGTGC TGTCGGAAGG AGAACGCTGT TGGCTGGGGG CCAGGCCGCC AAGACCCAGA 
GCCGCTCCCC AGCATCATCT TTTTGGGGTC TACCCCAGCA GGCCTGGGGA CTACCTGAGG 
CCCTACCCCA TGGGGGGGCA GGGAACCCGC CCCGCGGGAC GCAGTGAGCC AGCTGCCACG 
GGGGCTGCGA GCCCAGCTGG AGGTGTGGGG CTGAGGGGCA CAGCTGAGCA GCCAGCCGCC 
TCGTGGGACA GGGATGGTCC CGCTGGGCGG TCTGAGCTGC GGGGAGACGA TGACACTTAT 
CTTGGTGATG GAGGATCCAA GGAGTCTCTA GGTGAGGCTG GGACTCGTGA ACGCTCGGCC 
ACGGCTGCCA CGGCTGCTAC CACCTTCGTG GGCCCAAAGG AACCCAAACC AGAGACCCAA 
AGCAAGGGCC GGGCCAAGAC CAGGCGTCGT CGCCAGGTGG TGAAGGGGCA GGTAGGGGCC 
GTGCAGGGAG AGCCCGGTGT CTCTCCCCAG CGTCTCTGGC CGCGGCCGAA GCATCCCCTT 
GTGCACGGGG TCAGAATGAG TCTGTCGGAG GACGGCATCC AGAAGGGTGG AGGGGACCCC 
TCCTGGGAAG CAGAGTCCCA GGGAGGACTG CCTGTCTTGT ATTTCTCCGG GAGGCGGGGG 
CGGCTCCTGC TGCGTCCAGA AGTGCTGGCT GAGATTCCCC GGGAGGCGTT CACAGTGGAA 
GCCTGGGTGA GACCGGAGGG AGGACAGAAC AACCCAGCCA TCATTGCAGG TGTGTTTGAT 
AACTGCTCCC ACACTGTCAG TGACAAGGGC TGGGCCCTAG GGATCCGCTC AGGGAAGGAC 
ATAGGAAGGC GAGACGCTCG CTTCTTCTTC TCCCTTCGCA CCGACCGTGT GAAGAAAGCC 
ACCATTGTGA CTGGCAACAG TCGCTACCAA CCAGGCACAT GGACACACGT GGCAGCCACC 
TATGATGGAC ATCGTATGGC TCTGTATGTG GATGGCACTC AGGTGGCCAG TAGCCCAGAG 
CAATCTGGTC CCCTGAACAG CCCCTTCATG GCATCCTGTC GCTCTTTGCT TCTGGGGGGG 
GATAGCTCTG AGGACGGCCG CTATTTTCGT GGACACCTGG GCACCCTGGT CTTCTGGTCG 
ACTGCCCTCC CGCAAAGCCA TCTCCGGCAC AGTCCTCAGC GTCCAGCTGG AGTGGAAGAA 
TTGGCCTCCC TCATCCTGAC AGCCAGTTTT GAGCCCTTGG AAGAACAGTG GGCCCCCTTT 
AGAGATGGGG AGTTCCCACG GCGTGAGGTT CTGCATGGCT TTGAGCCTGA GCCTGAGATT 
GTGTCACCTC TGAAGCCCCC ACTCTGTGGA CGGACGGCTT GTGACAACGT GGAACTGATC 
TCCCACTACA ACGGGCACCG GCCCCTCCAG GGAGAGAAGG TGATCCGCTA CCGAGTGGTA 
AACATCTGTG ACGATGAGGG CCTGAACCCC ACTGTGAGCG AGGAGCAGAT CAGCCGGCAG 
CACAAGGCGC TGAACCATGC CTTCAGCCGC TACAACATCA GCTGGCAGCT GAGCGTCCAC 
CAGGTCCACA ACTCCACCCT GCGCCACCGG GTTGTGCTTG TGAACTGCGA GCCCAGCAAG 
ATTGGCAATG ACCACTGTGA CCCTGAGTGT GAGCACCCAC TCACAGGCTA TGACGGGGGC 
GACTGCCGCC TACATGGCCG GTGCTACTCC TGGAACCGCA GGGACGGGCT CTGCCACGTG 
GAGTGCAACA ACATGCTGAA CGACTTTGAC GACGGGGACT GCTGCGACCC CGAGGTGGCC 
GATGTGCACA AGACCTGCTT TGACCCCGAC TCACCCAAGA GGGCATACAT GAGTGTGAAG 
GAGCTGAAGG AGGCACTGCA GCTCAACAGT ACTCACTTCC TCAATGTCTA CTTTGCCAGC 
TCCGTGCGGG AAGACCTTGC AGGCGCTGCC ACCTGGCCTT GGGACAAGGA AGCCATCAGT 
CACCTGGGTG GCGTTGTCCT CAACCCAGCC TATTATGGCA TGCCTGGCCA TACCAACATT 
ATGATCCACG AGGTAGGGCA TGTCCTGGGA CTCTACCATG TCTTCAAAGG GGTCAGTGAG 
AGGGAATCCT GTGATGACCC CTGTAGGGAG ACAGTGCCAT CCATGGATAC GGGAGACCTC 
TGTGCCGACA CCGCCCCTAC TCCCAAGAGT AAGCTGTGCC AGGACCCAGA GCCTACCAAC 
GATACCTGTG GCTTCACCCA CTTCCCGGGG GCTCCGTTCA GCAACTACAT GAGCTATACA 
GATGATGATT GCACCGACAG CTTCACCCCT AACCAGGTGG CCCGGATGCA TTGCTATTTG 
GACCTTGTGT ATCAGCAGTG GAGTCAAAGC CGGAAGCCCA CCCCCATTCC CATCCCACCC 
ATGGTCATCG GGCAGACCCA CACCTCCCTC ACCATCCACT GGCTTCCTCC AATCAGTGGG 
GTTGTACACG ACAGGGTCCC AGGCAGCGTG TGTGGTGCCT GCACTGAAGA TGGGACATTC 
CGTCAGTACG TGCACGCAGC TTCCTCCCGG CGTGTGTGTG ACTCCTCAGG TTACTGGACT 
CCAGAGGAGG CTGTGGGGCC TCCAGACGTG GACCAGCCCT GTGAGCCGAG CTTGCAGGCC 
TGGAGTCCCG AGCTCCACCT GTACCATATG AACATGACGG TCCCCTGCCC TTCGGAAGGC 
TGTAGCCTGG AGCTGCTCTT CCAGCACCCG GTCCAAGCTG ACACCCTTAC CCTATGGGTC 
ACCTACCTCT CCATGGACTC CTCCCAGGCA CTCTTTGACA CGGAGATCTT GCTGGAACAC 
CAGGAGTCTG TGCACCTGGG CCCCTTAGAC ACTTTCTGTG ACACCCCACT CACTATCAAA 
CTGCATGTGG ATGGGAAGGT CTCTGGGGTG AAGGTCTACA CCTTTGATGA GCGGATGGAG 
ATTGACGCAG CTCTGCTAAC TTCCCAGCCC CATAGTCCCC TGTGCTCTGG CTGCAGGCCT 
GTGAGGGAGG TCACACCTGG GCAGCTGTAT CGGTACCAAG TTCAAGCTGG AGCTGGAACA 
GAACTAGGAG AAGCCTCGCC TCCGCTGAGC CACATCCATG GAGCTCCTTA CTGTGGAGAC 
GGGAAGGTGG CAGGGAGCCT GGGAGAAGAA TGTGATGATG GGGACCTTCT GAGCGGAGAT 
GGCTGCTCAA GGGCGTGCCA GCTGGAGGAA GGCTTCCACT GCGCAGGGGA GCCAAGCCTA 
TGTTACATAT ATGAGGGAGA TGGGATATGT GAACCTTTTG AGAAGGAAAC CAGCATCATA 
GACTGTGGCC TCTATACTCC CAAAGGATAT GTGGATCAGT GGGCCACCCA AGCTTATTCC 
TCTCATGAAG ACAAGAAGTG TCCTGCTTCC TTGGTAACTG GAGAACCTCA TTCCATGATT 
TGCACGTCAT ACCATCCAGA CTTACCAGAG CACCGTCCTC TCACTGGCTG GTTTCCCTGT 
GTCACCAATG GAAACAGACC TCAGGATAAA AGGAGTGAGC AGACAGAAGG TAGCCTGGAG 
AGGGAGGAGG AGGTTTGGCT CAAAGTGTGT TTCAGTAGAC CAGGAGTGGC CACGACAATT 
TTCATTTTCT TGACATCTGA TGGCCCGGCA CTTGGAGAAC ATCGGCGGCC AACAGTGACT 
GTTCACCTGA CTGATGTCAG TGGGAACAAC CACTCTCTTG GAACCTATGA ACTGTCATGC 
CAGCATAACC CGCTGGTTAT CAATGTGAGC CATCACCCGA ATGTCCTCTC CCACCATACC 
TCTTCAGTGC TGCTGAATTT CTCATCCCCA TTGGTGGGCA TCTCAGCGGT GGCTCTAAGG 
ACATCCTCCC ACATAAGTTC ATCAACTCTC AATAACTGCA TCCCAGAGCA TGAGGGACAA 
AATCATCAGG GACAGAGCTG TGTCCACCGG CCCTGTGGGG AGCAGGGTAG CTGTGCGCCA 
TTGCTGCTTG ATCATGCGGA TATGGTGAAC TGTACCTCTG GTGGCCCAGG TCACATGAAG 
TGTGCTATCA CCTGTCAAAG GGGGTTTGCC CTTCAGGCCA GCAGCGGGCA ATACCTCAGG 
CCCGTGCAGA AGGAAATTCT GCTCACCTGT TCCTCTGGGC ACTGGGACCG GGCTGTGAGC 
TGCATGCCCC TTGATTGTGG CGTTCCCGAC CCATCTTTGG TGAACTACGC AAACTTCTCC 
TGCTCAGAGG GAACAGACTT TCTGAAGCGC TGTTCAATCT CCTGTGTCCC ACCAGCCAAG 
CTCCAAGGAC TGAACCCATG GCTGACTTGT CTTGAAGACG GGCTCTGGTC CCTCCCGGAA 
GCCTACTGCA AGTTGGAGTG TGACGCCCCC CCTGTAATCC CGAATGCCAG CCTGCTCTTG 
CCTCACTGCC TCCAGGGCAA CCATGACGTG GGCTCCGTCT GTAGATACGA ATGCAAACCA 
GGCTACTATG CCGAGCCAAG TGCCGAGGGT AAAGTCAGGA GCAATTTCCT GAAGATACAG 
TGCCTGGAGG GTGGCATCTG GGAGCAAGGC AGCTGCGTTC CAGTGGTGTG TGAGCCCCCT 
TCCCCTGTCT TTGAAGGCAT GTATGAATGT ACCAATGGCT TTGAACTCAA CAGCCGGTGT 
GTGCTCAGCT GTAACCAGGA AAGCGGAAGG GTTCCTATCC TCTGCACTAA GGAGGGACTG 
TGGACTGAGG AGTTCAAGTT GTGTGAGAAC CTGCAAGGGG AGTGCCCGCC ACCCCCGTCG 
GAGCTGAATT TTGTGGAGTA CAAGTGTGAA CAGGGATACG GGATTGGTGC TGTGTGTTCC 
CCATCATGTG TGATTCCCCC TAGTGACCCC GTAGTTCTGC CTGAGAACGT CACTGCTGAC 
ACGCTGGAGC ACTGGATGGA ACCTGTCAAA GTCCAGAGCA TTGTGTGCAC TGGACAGCGT 
CAATGGCACC CACCCCCCTT CCTCATCCAC TGCATCCAGT CGTGTGAGCC TTTCCAAGCC 
GATGGGTGGT GCGATACCAT CAACAACCGG GCCTACTGCC ACTATGATGG GGGAGACTGC 
TGCTCTTCTA CTCTGTCCTC CAAGAAGGTC ATTCCATTTG CTGCTGACTG TGACCTGGAT 
GAATGCACCT GCCGGGACCC CAAGGCTGAA GAAAATCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPPA2 ( XM_023248030.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023103798.1 Felis catus pappalysin 2 (PAPPA2), mRNA.

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CloneID	OFb68435
Clone ID Related Accession (Same CDS sequence)	XM_023248030.1
Accession Version	XM_023248030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	pappalysin-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018739.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGATGGGCC CAAAGATCCT GAGAATAAGC CTGGTGATTC TGGCCGGGTG GGCGCTCGGC 
ACTGCCGGCT CTGAGCCGGG CTGGACACGC AAGAGGTCCC TGGCTCAGAG GGGACGCCTG 
AACCAGGTGC TGTCGGAAGG AGAACGCTGT TGGCTGGGGG CCAGGCCGCC AAGACCCAGA 
GCCGCTCCCC AGCATCATCT TTTTGGGGTC TACCCCAGCA GGCCTGGGGA CTACCTGAGG 
CCCTACCCCA TGGGGGGGCA GGGAACCCGC CCCGCGGGAC GCAGTGAGCC AGCTGCCACG 
GGGGCTGCGA GCCCAGCTGG AGGTGTGGGG CTGAGGGGTA CAGCTGAGCA GCCAGCCGCC 
TCGTGGGACA GGGATGGTCC CGCTGGGCGG TCTGAGCTGC GGGGAGACGA TGACACTTAT 
CTTGGTGATG GAGGATCCAA GGAGTCTCTA GGTGAGGCTG GGACTCGTGA ACGCTCGGCC 
ACGGCTGCCA CGGCTGCTAC CACCTTCGTG GGCCCAAAGG AACCCAAACC AGAGACCCAA 
AGCAAGGGCC GGGCCAAGAC CAGGCGTCGT CGCCAGGTGG TGAAGGGGCA GGTAGGGGCC 
GTGCAGGGAG AGCCCGGTGT CTCTCCCCAG CGTCTCTGGC CGCGGCCGAA GCATCCCCTT 
GTGCACGGGG TCAGAACGAG TCCGTCGGAG GACGGCATCC AGAAGGGTGG AGGGGACCCC 
TCCTGGGAAG CAGAGTCCCA GGGAGGACTG CCTGTCTTGT ATTTCTCCGG GAGGCGGGGG 
CGGCTCCTGC TGCGTCCAGA AGTGCTGGCT GAGATTCCCC GGGAGGCGTT CACAGTGGAA 
GCCTGGGTGA GACCGGAGGG AGGACAGAAC AACCCAGCCA TCATTGCAGG TGTGTTTGAT 
AACTGCTCCC ACACTGTCAG TGACAAGGGC TGGGCCCTAG GGATCCGCTC AGGGAAGGAC 
ATAGGAAGGC GAGACGCTCG CTTCTTCTTC TCCCTTCGCA CCGACCGTGT GAAGAAAGCC 
ACCATTGTGA CTGGCAACAG TCGCTACCAA CCAGGCACAT GGACACACGT GGCAGCCACC 
TATGATGGAC ATCGTATGGC TCTGTATGTG GATGGCACTC AGGTGGCCAG TAGCCCAGAG 
CAATCTGGTC CCCTGAACAG CCCCTTCATG GCATCCTGTC GCTCTTTGCT TCTGGGGGGG 
GATAGCTCTG AGGACGGCCG CTATTTTCGT GGACACCTGG GCACCCTGGT CTTCTGGTCG 
ACCGCCCTCC CGCAAAGCCA TCTCCGGCAC AGTCCTCAGC GTCCAGCTGG AGTGGAAGAA 
TTGGCCTCCC TCATCCTGAC AGCCAGTTTT GAGCCCTTGG AAGAACAGTG GGCCCCCTTT 
AGAGATGGGG AGTTCCCACG GCGTGAGGTT CTGCATGGCT TTGAGCCTGA GCCTGAGATT 
GTGTCACCTC TGAAGCCCCC ACTCTGTGGA CGGACGGCTT GTGACAACGT GGAACTGATC 
TCCCACTACA ACGGGCACCG GCCCCTCCAG GGAGAGAAGG TGATCCGCTA CCGAGTGGTA 
AACATCTGTG ACGATGAGGG CCTGAACCCC ACCGTGAGCG AGGAGCAGAT CAGCCGGCAG 
CACAAGGCGC TGAACCATGC CTTCAGCCGC TACAACATCA GCTGGCAGCT GAGCGTCCAC 
CAGGTCCACA ACTCCACCCT GCGCCACCGG GTTGTGCTTG TGAACTGCGA GCCCAGCAAG 
ATTGGCAATG ACCACTGTGA CCCTGAGTGT GAGCACCCAC TCACAGGCTA TGACGGTGGC 
GACTGCCGCC TACATGGCCG GTGCTACTCC TGGAACCGCA GGGACGGGCT CTGCCACGTG 
GAGTGCAACA ACATGCTGAA CGACTTTGAC GACGGGGACT GCTGCGACCC CGAGGTGGCC 
GATGTGCACA AGACCTGCTT TGACCCCGAC TCACCCAAGA GGGCATACAT GAGTGTGAAG 
GAGCTGAAGG AGGCACTGCA GCTCAACAGT ACTCACTTCC TCAATGTCTA CTTTGCCAGC 
TCCGTGCGGG AAGACCTTGC AGGCGCTGCC ACCTGGCCTT GGGACAAGGA AGCCATCAGT 
CACCTGGGTG GCGTTGTCCT CAACCCAGCC TATTATGGCA TGCCTGGCCA TACCAACATT 
ATGATCCACG AGGTAGGGCA TGTCCTGGGA CTCTACCATG TCTTCAAAGG GGTCAGTGAG 
AGGGAATCCT GTGATGACCC CTGTAGGGAG ACAGTGCCAT CCATGGATAC GGGAGACCTC 
TGTGCCGACA CCGCCCCTAC TCCCAAGAGT AAGCTGTGCC AGGACCCAGA GCCTACCAAC 
GATACCTGTG GCTTCACCCA CTTCCCGGGG GCTCCGTTCA GCAACTACAT GAGCTATACA 
GATGATGATT GCACCGACAG CTTCACCCCT AACCAGGTGG CCCGGATGCA TTGCTATTTG 
GACCTTGTGT ATCAGCAGTG GAGTCAAAGC CGGAAGCCCA CCCCCATTCC CATCCCACCC 
ATGGTCATCG GGCAGACCCA CACCTCCCTC ACCATCCACT GGCTTCCTCC AATCAGTGGG 
GTTGTACACG ACAGGGTCCC AGGCAGCGTG TGTGGTGCCT GCACTGAAGA TGGGACATTC 
CGTCAGTACG TGCACGCAGC TTCCTCCCGG CGTGTGTGTG ACTCCTCAGG TTACTGGACT 
CCAGAGGAGG CTGTGGGGCC TCCAGACGTG GACCAGCCCT GTGAGCCGAG CTTGCAGGCC 
TGGAGTCCCG AGCTCCACCT GTACCATATG AACATGACGG TCCCCTGCCC TTCGGAAGGC 
TGTAGCCTGG AGCTGCTCTT CCAGCACCCG GTCCAAGCTG ACACCCTTAC CCTATGGGTC 
ACCTACCTCT CCATGGACTC CTCCCAGGCA CTCTTTGACA CGGAGATCTT GCTGGAACAC 
CAGGAGTCTG TGCACCTGGG CCCCTTAGAC ACTTTCTGTG ACACCCCACT CACTATCAAA 
CTGCATGTGG ATGGGAAGGT CTCTGGGGTG AAGGTCTACA CCTTTGATGA GCGGATGGAG 
ATTGACGCAG CTCTGCTAAC TTCCCAGCCC CATAGTCCCC TGTGCTCTGG CTGCAGGCCT 
GTGAGGTACC AGGTTCTTCG TGAGCCCCCA TTTGCCAATG GCTTGCCCAT GGTGGTGACA 
AATCCTCACA GGAAGTTCAC GGACGTGGAG GTCACACCTG GGCAGCTGTA TCGGTACCAA 
GTTCAAGCTG GAGCTGGAAC AGAACTAGGA GAAGCCTCGC CTCCGCTGAG CCACATCCAT 
GGAGCTCCTT ACTGTGGAGA CGGGAAGGTG GCAGGGAGCC TGGGAGAAGA ATGTGATGAT 
GGGGACCTTC TGAGCGGAGA TGGCTGCTCA AGGGCGTGCC AGCTGGAGGA AGGCTTCCAC 
TGCGCAGGGG AGCCAAGCCT ATGTTACATA TATGAGGGAG ATGGGATATG TGAACCTTTT 
GAGAAGGAAA CCAGCATCAT AGACTGTGGC CTCTATACTC CCAAAGGATA TGTGGATCAG 
TGGGCCACCC GAGCTTATTC CTCTCATGAA GACAAGAAGT GTCCTGCTTC CTTGGTAACT 
GGAGAACCTC ATTCCATGAT TTGCACGTCA TACCATCCAG ACTTACCAGA GCACCGTCCT 
CTCACTGGCT GGTTTCCCTG TGTCACCAAT GGAAACAGAC CTCAGGATAA AAGGAGTGAG 
CAGACAGAAG GTAGCCTGGA GAGGGAGGAG GAGGTTTGGC TCAAAGTGTG TTTCAGTAGA 
CCAGGAGTGG CCACGACAAT TTTCATTTTC TTGACATCTG ATGGCCCGGC ACTTGGAGAA 
CATCGGCGGC CAACAGTGAC TGTTCACCTG ACTGATGTCA GTGGGAACAA CCACTCTCTT 
GGAACCTATG AACTGTCATG CCAGCATAAC CCGCTGGTTA TCAATGTGAG CCATCACCCG 
AATGTCCTCT CCCACCATAC CTCTTCAGTG CTGCTGAATT TCTCATCCCC ATTGGTGGGC 
ATCTCAGCGG TGGCTCTAAG GACATCCTCC CACATAAGTT CATCAACTCT CAATAACTGC 
ATCCCAGAGC ATGAGGGACA AAATCATCAG GGACAGAGCT GTGTCCACCG GCCCTGTGGG 
GAGCAGGGTA GCTGTGCGCC ATTGCTGCTT GATCATGCGG ATATGGTGAA CTGTACCTCT 
GGTGGCCCAG GTCACATGAA GTGTGCTATC ACCTGTCAAA GGGGGTTTGC CCTTCAGGCC 
AGCAGCGGGC AATACCTCAG GCCCGTGCAG AAGGAAATTC TGCTCACCTG TTCCTCTGGG 
CACTGGGACC GGGCTGTGAG CTGCATGCCC CTTGATTGTG GCGTTCCCGA CCCATCTTTG 
GTGAACTACG CAAACTTCTC CTGCTCAGAG GGAACAACAG ACTTTCTGAA GCGCTGTTCA 
ATCTCCTGTG TCCCACCAGC CAAGCTCCAA GGACTGAACC CATGGCTGAC TTGTCTTGAA 
GACGGGCTCT GGTCCCTCCC GGAAGCCTAC TGCAAGTTGG AGTGTGACGC CCCCCCTGTA 
ATCCCGAATG CCAGCCTGCT CTTGCCTCAC TGCCTCCAGG GCAACCATGA CGTGGGCTCC 
GTCTGTAGAT ACGAATGCAA ACCAGGCTAC TATGCCGAGC CAAGTGCCGA GGGTAAAGTC 
AGGAGCAATT TCCTGAAGAT ACAGTGCCTG GAGGGTGGCA TCTGGGAGCA AGGCAGCTGC 
GTTCCAGTGG TGTGTGAGCC CCCTTCCCCT GTCTTTGAAG GCATGTATGA ATGTACCAAT 
GGCTTTGAAC TCAACAGCCG GTGTGTGCTC AGCTGTAACC AGGAAAGCGG AAGGGTTCCT 
ATCCTCTGCA CTAAGGAGGG ACTGTGGACT GAGGAGTTCA AGTTGTGTGA GAACCTGCAA 
GGGGAGTGCC CGCCACCCCC GTCGGAGCTG AATTTTGTGG AGTACAAGTG TGAACAGGGA 
TACGGGATTG GTGCTGTGTG TTCCCCATCA TGTGTGATTC CCCCTAGTGA CCCCGTAGTT 
CTGCCTGAGA ACGTCACTGC TGACACGCTG GAGCACTGGA TGGAACCTGT CAAAGTCCAG 
AGCATTGTGT GCACTGGACA GCGTCAATGG CACCCACCCC CCTTCCTCAC CACTGAATCC 
AGTCGTGTGA GATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023103798.1
CDS	1007..6184
Translation	MMGPKILRISLVILAGWALGTAGSEPGWTRKRSLAQRGRLNQVLSEGERCWLGARPPRPRAAPQHHLFGVYPSRPGDYLRPYPMGGQGTRPAGRSEPAATGAASPAGGVGLRGTAEQPAASWDRDGPAGRSELRGDDDTYLGDGGSKESLGEAGTRERSATAATAATTFVGPKEPKPETQSKGRAKTRRRRQVVKGQVGAVQGEPGVSPQRLWPRPKHPLVHGVRTSPSEDGIQKGGGDPSWEAESQGGLPVLYFSGRRGRLLLRPEVLAEIPREAFTVEAWVRPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDIGRRDARFFFSLRTDRVKKATIVTGNSRYQPGTWTHVAATYDGHRMALYVDGTQVASSPEQSGPLNSPFMASCRSLLLGGDSSEDGRYFRGHLGTLVFWSTALPQSHLRHSPQRPAGVEELASLILTASFEPLEEQWAPFRDGEFPRREVLHGFEPEPEIVSPLKPPLCGRTACDNVELISHYNGHRPLQGEKVIRYRVVNICDDEGLNPTVSEEQISRQHKALNHAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLHGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVADVHKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGAATWPWDKEAISHLGGVVLNPAYYGMPGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMDTGDLCADTAPTPKSKLCQDPEPTNDTCGFTHFPGAPFSNYMSYTDDDCTDSFTPNQVARMHCYLDLVYQQWSQSRKPTPIPIPPMVIGQTHTSLTIHWLPPISGVVHDRVPGSVCGACTEDGTFRQYVHAASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLYHMNMTVPCPSEGCSLELLFQHPVQADTLTLWVTYLSMDSSQALFDTEILLEHQESVHLGPLDTFCDTPLTIKLHVDGKVSGVKVYTFDERMEIDAALLTSQPHSPLCSGCRPVRYQVLREPPFANGLPMVVTNPHRKFTDVEVTPGQLYRYQVQAGAGTELGEASPPLSHIHGAPYCGDGKVAGSLGEECDDGDLLSGDGCSRACQLEEGFHCAGEPSLCYIYEGDGICEPFEKETSIIDCGLYTPKGYVDQWATRAYSSHEDKKCPASLVTGEPHSMICTSYHPDLPEHRPLTGWFPCVTNGNRPQDKRSEQTEGSLEREEEVWLKVCFSRPGVATTIFIFLTSDGPALGEHRRPTVTVHLTDVSGNNHSLGTYELSCQHNPLVINVSHHPNVLSHHTSSVLLNFSSPLVGISAVALRTSSHISSSTLNNCIPEHEGQNHQGQSCVHRPCGEQGSCAPLLLDHADMVNCTSGGPGHMKCAITCQRGFALQASSGQYLRPVQKEILLTCSSGHWDRAVSCMPLDCGVPDPSLVNYANFSCSEGTTDFLKRCSISCVPPAKLQGLNPWLTCLEDGLWSLPEAYCKLECDAPPVIPNASLLLPHCLQGNHDVGSVCRYECKPGYYAEPSAEGKVRSNFLKIQCLEGGIWEQGSCVPVVCEPPSPVFEGMYECTNGFELNSRCVLSCNQESGRVPILCTKEGLWTEEFKLCENLQGECPPPPSELNFVEYKCEQGYGIGAVCSPSCVIPPSDPVVLPENVTADTLEHWMEPVKVQSIVCTGQRQWHPPPFLTTESSRVRY

Target ORF information:

RefSeq Version	XM_023248030.1
Organism	Felis catus(domestic cat)
Definition	Felis catus pappalysin 2 (PAPPA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023248030.1

ORF Insert Sequence:

ATGATGGGCC CAAAGATCCT GAGAATAAGC CTGGTGATTC TGGCCGGGTG GGCGCTCGGC 
ACTGCCGGCT CTGAGCCGGG CTGGACACGC AAGAGGTCCC TGGCTCAGAG GGGACGCCTG 
AACCAGGTGC TGTCGGAAGG AGAACGCTGT TGGCTGGGGG CCAGGCCGCC AAGACCCAGA 
GCCGCTCCCC AGCATCATCT TTTTGGGGTC TACCCCAGCA GGCCTGGGGA CTACCTGAGG 
CCCTACCCCA TGGGGGGGCA GGGAACCCGC CCCGCGGGAC GCAGTGAGCC AGCTGCCACG 
GGGGCTGCGA GCCCAGCTGG AGGTGTGGGG CTGAGGGGTA CAGCTGAGCA GCCAGCCGCC 
TCGTGGGACA GGGATGGTCC CGCTGGGCGG TCTGAGCTGC GGGGAGACGA TGACACTTAT 
CTTGGTGATG GAGGATCCAA GGAGTCTCTA GGTGAGGCTG GGACTCGTGA ACGCTCGGCC 
ACGGCTGCCA CGGCTGCTAC CACCTTCGTG GGCCCAAAGG AACCCAAACC AGAGACCCAA 
AGCAAGGGCC GGGCCAAGAC CAGGCGTCGT CGCCAGGTGG TGAAGGGGCA GGTAGGGGCC 
GTGCAGGGAG AGCCCGGTGT CTCTCCCCAG CGTCTCTGGC CGCGGCCGAA GCATCCCCTT 
GTGCACGGGG TCAGAACGAG TCCGTCGGAG GACGGCATCC AGAAGGGTGG AGGGGACCCC 
TCCTGGGAAG CAGAGTCCCA GGGAGGACTG CCTGTCTTGT ATTTCTCCGG GAGGCGGGGG 
CGGCTCCTGC TGCGTCCAGA AGTGCTGGCT GAGATTCCCC GGGAGGCGTT CACAGTGGAA 
GCCTGGGTGA GACCGGAGGG AGGACAGAAC AACCCAGCCA TCATTGCAGG TGTGTTTGAT 
AACTGCTCCC ACACTGTCAG TGACAAGGGC TGGGCCCTAG GGATCCGCTC AGGGAAGGAC 
ATAGGAAGGC GAGACGCTCG CTTCTTCTTC TCCCTTCGCA CCGACCGTGT GAAGAAAGCC 
ACCATTGTGA CTGGCAACAG TCGCTACCAA CCAGGCACAT GGACACACGT GGCAGCCACC 
TATGATGGAC ATCGTATGGC TCTGTATGTG GATGGCACTC AGGTGGCCAG TAGCCCAGAG 
CAATCTGGTC CCCTGAACAG CCCCTTCATG GCATCCTGTC GCTCTTTGCT TCTGGGGGGG 
GATAGCTCTG AGGACGGCCG CTATTTTCGT GGACACCTGG GCACCCTGGT CTTCTGGTCG 
ACCGCCCTCC CGCAAAGCCA TCTCCGGCAC AGTCCTCAGC GTCCAGCTGG AGTGGAAGAA 
TTGGCCTCCC TCATCCTGAC AGCCAGTTTT GAGCCCTTGG AAGAACAGTG GGCCCCCTTT 
AGAGATGGGG AGTTCCCACG GCGTGAGGTT CTGCATGGCT TTGAGCCTGA GCCTGAGATT 
GTGTCACCTC TGAAGCCCCC ACTCTGTGGA CGGACGGCTT GTGACAACGT GGAACTGATC 
TCCCACTACA ACGGGCACCG GCCCCTCCAG GGAGAGAAGG TGATCCGCTA CCGAGTGGTA 
AACATCTGTG ACGATGAGGG CCTGAACCCC ACCGTGAGCG AGGAGCAGAT CAGCCGGCAG 
CACAAGGCGC TGAACCATGC CTTCAGCCGC TACAACATCA GCTGGCAGCT GAGCGTCCAC 
CAGGTCCACA ACTCCACCCT GCGCCACCGG GTTGTGCTTG TGAACTGCGA GCCCAGCAAG 
ATTGGCAATG ACCACTGTGA CCCTGAGTGT GAGCACCCAC TCACAGGCTA TGACGGTGGC 
GACTGCCGCC TACATGGCCG GTGCTACTCC TGGAACCGCA GGGACGGGCT CTGCCACGTG 
GAGTGCAACA ACATGCTGAA CGACTTTGAC GACGGGGACT GCTGCGACCC CGAGGTGGCC 
GATGTGCACA AGACCTGCTT TGACCCCGAC TCACCCAAGA GGGCATACAT GAGTGTGAAG 
GAGCTGAAGG AGGCACTGCA GCTCAACAGT ACTCACTTCC TCAATGTCTA CTTTGCCAGC 
TCCGTGCGGG AAGACCTTGC AGGCGCTGCC ACCTGGCCTT GGGACAAGGA AGCCATCAGT 
CACCTGGGTG GCGTTGTCCT CAACCCAGCC TATTATGGCA TGCCTGGCCA TACCAACATT 
ATGATCCACG AGGTAGGGCA TGTCCTGGGA CTCTACCATG TCTTCAAAGG GGTCAGTGAG 
AGGGAATCCT GTGATGACCC CTGTAGGGAG ACAGTGCCAT CCATGGATAC GGGAGACCTC 
TGTGCCGACA CCGCCCCTAC TCCCAAGAGT AAGCTGTGCC AGGACCCAGA GCCTACCAAC 
GATACCTGTG GCTTCACCCA CTTCCCGGGG GCTCCGTTCA GCAACTACAT GAGCTATACA 
GATGATGATT GCACCGACAG CTTCACCCCT AACCAGGTGG CCCGGATGCA TTGCTATTTG 
GACCTTGTGT ATCAGCAGTG GAGTCAAAGC CGGAAGCCCA CCCCCATTCC CATCCCACCC 
ATGGTCATCG GGCAGACCCA CACCTCCCTC ACCATCCACT GGCTTCCTCC AATCAGTGGG 
GTTGTACACG ACAGGGTCCC AGGCAGCGTG TGTGGTGCCT GCACTGAAGA TGGGACATTC 
CGTCAGTACG TGCACGCAGC TTCCTCCCGG CGTGTGTGTG ACTCCTCAGG TTACTGGACT 
CCAGAGGAGG CTGTGGGGCC TCCAGACGTG GACCAGCCCT GTGAGCCGAG CTTGCAGGCC 
TGGAGTCCCG AGCTCCACCT GTACCATATG AACATGACGG TCCCCTGCCC TTCGGAAGGC 
TGTAGCCTGG AGCTGCTCTT CCAGCACCCG GTCCAAGCTG ACACCCTTAC CCTATGGGTC 
ACCTACCTCT CCATGGACTC CTCCCAGGCA CTCTTTGACA CGGAGATCTT GCTGGAACAC 
CAGGAGTCTG TGCACCTGGG CCCCTTAGAC ACTTTCTGTG ACACCCCACT CACTATCAAA 
CTGCATGTGG ATGGGAAGGT CTCTGGGGTG AAGGTCTACA CCTTTGATGA GCGGATGGAG 
ATTGACGCAG CTCTGCTAAC TTCCCAGCCC CATAGTCCCC TGTGCTCTGG CTGCAGGCCT 
GTGAGGTACC AGGTTCTTCG TGAGCCCCCA TTTGCCAATG GCTTGCCCAT GGTGGTGACA 
AATCCTCACA GGAAGTTCAC GGACGTGGAG GTCACACCTG GGCAGCTGTA TCGGTACCAA 
GTTCAAGCTG GAGCTGGAAC AGAACTAGGA GAAGCCTCGC CTCCGCTGAG CCACATCCAT 
GGAGCTCCTT ACTGTGGAGA CGGGAAGGTG GCAGGGAGCC TGGGAGAAGA ATGTGATGAT 
GGGGACCTTC TGAGCGGAGA TGGCTGCTCA AGGGCGTGCC AGCTGGAGGA AGGCTTCCAC 
TGCGCAGGGG AGCCAAGCCT ATGTTACATA TATGAGGGAG ATGGGATATG TGAACCTTTT 
GAGAAGGAAA CCAGCATCAT AGACTGTGGC CTCTATACTC CCAAAGGATA TGTGGATCAG 
TGGGCCACCC GAGCTTATTC CTCTCATGAA GACAAGAAGT GTCCTGCTTC CTTGGTAACT 
GGAGAACCTC ATTCCATGAT TTGCACGTCA TACCATCCAG ACTTACCAGA GCACCGTCCT 
CTCACTGGCT GGTTTCCCTG TGTCACCAAT GGAAACAGAC CTCAGGATAA AAGGAGTGAG 
CAGACAGAAG GTAGCCTGGA GAGGGAGGAG GAGGTTTGGC TCAAAGTGTG TTTCAGTAGA 
CCAGGAGTGG CCACGACAAT TTTCATTTTC TTGACATCTG ATGGCCCGGC ACTTGGAGAA 
CATCGGCGGC CAACAGTGAC TGTTCACCTG ACTGATGTCA GTGGGAACAA CCACTCTCTT 
GGAACCTATG AACTGTCATG CCAGCATAAC CCGCTGGTTA TCAATGTGAG CCATCACCCG 
AATGTCCTCT CCCACCATAC CTCTTCAGTG CTGCTGAATT TCTCATCCCC ATTGGTGGGC 
ATCTCAGCGG TGGCTCTAAG GACATCCTCC CACATAAGTT CATCAACTCT CAATAACTGC 
ATCCCAGAGC ATGAGGGACA AAATCATCAG GGACAGAGCT GTGTCCACCG GCCCTGTGGG 
GAGCAGGGTA GCTGTGCGCC ATTGCTGCTT GATCATGCGG ATATGGTGAA CTGTACCTCT 
GGTGGCCCAG GTCACATGAA GTGTGCTATC ACCTGTCAAA GGGGGTTTGC CCTTCAGGCC 
AGCAGCGGGC AATACCTCAG GCCCGTGCAG AAGGAAATTC TGCTCACCTG TTCCTCTGGG 
CACTGGGACC GGGCTGTGAG CTGCATGCCC CTTGATTGTG GCGTTCCCGA CCCATCTTTG 
GTGAACTACG CAAACTTCTC CTGCTCAGAG GGAACAACAG ACTTTCTGAA GCGCTGTTCA 
ATCTCCTGTG TCCCACCAGC CAAGCTCCAA GGACTGAACC CATGGCTGAC TTGTCTTGAA 
GACGGGCTCT GGTCCCTCCC GGAAGCCTAC TGCAAGTTGG AGTGTGACGC CCCCCCTGTA 
ATCCCGAATG CCAGCCTGCT CTTGCCTCAC TGCCTCCAGG GCAACCATGA CGTGGGCTCC 
GTCTGTAGAT ACGAATGCAA ACCAGGCTAC TATGCCGAGC CAAGTGCCGA GGGTAAAGTC 
AGGAGCAATT TCCTGAAGAT ACAGTGCCTG GAGGGTGGCA TCTGGGAGCA AGGCAGCTGC 
GTTCCAGTGG TGTGTGAGCC CCCTTCCCCT GTCTTTGAAG GCATGTATGA ATGTACCAAT 
GGCTTTGAAC TCAACAGCCG GTGTGTGCTC AGCTGTAACC AGGAAAGCGG AAGGGTTCCT 
ATCCTCTGCA CTAAGGAGGG ACTGTGGACT GAGGAGTTCA AGTTGTGTGA GAACCTGCAA 
GGGGAGTGCC CGCCACCCCC GTCGGAGCTG AATTTTGTGG AGTACAAGTG TGAACAGGGA 
TACGGGATTG GTGCTGTGTG TTCCCCATCA TGTGTGATTC CCCCTAGTGA CCCCGTAGTT 
CTGCCTGAGA ACGTCACTGC TGACACGCTG GAGCACTGGA TGGAACCTGT CAAAGTCCAG 
AGCATTGTGT GCACTGGACA GCGTCAATGG CACCCACCCC CCTTCCTCAC CACTGAATCC 
AGTCGTGTGA GATATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PAPPA2 cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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