ORF » Species Summary » Danio rerio » TJP1B cDNA ORF clone

TJP1B cDNA ORF clone, Danio rerio(Zebrafish)

The following TJP1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa44626 XM_021470733.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa44627 XM_009298137.3
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa44628 XM_021470734.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa44629 XM_021470736.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa44630 XM_021470737.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa44631 XM_009298141.3
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa57885 XM_017354175.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa57886 XM_017354176.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa57887 XM_017354177.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa57888 XM_017354178.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa141490 XM_021470724.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa141491 XM_021470731.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44616 XM_021470723.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44617 XM_021470725.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44618 XM_021470726.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44619 XM_021470727.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44620 XM_009298130.3
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44621 XM_021470728.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44622 XM_009298132.3
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44623 XM_021470730.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44624 XM_021470732.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44625 XM_009298135.3
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa57879 XM_017354168.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa57880 XM_017354169.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa57881 XM_017354170.2
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa57882 XM_017354171.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa57883 XM_017354172.2
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa57884 XM_017354173.1
Latest version!		 Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
View Old Accession Versions >>
ODa44620 XM_009298130.2 Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44625 XM_009298135.2 Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa44631 XM_009298141.2 Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa44627 XM_009298137.2 Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
ODa44622 XM_009298132.2 Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa57883 XM_017354172.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
ODa57881 XM_017354170.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    TJP1B ( XM_021470733.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326408.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA.
    Price & Availability↑
    CloneID ODa44626
    Clone ID Related Accession (Same CDS sequence) XM_021470733.1
    Accession Version XM_021470733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5913bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
    ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
    TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
    AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
    GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
    AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
    GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
    GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
    GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
    CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
    ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
    GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
    TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
    GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
    CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
    TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
    TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
    TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
    GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
    CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
    CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
    CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
    AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
    ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
    ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
    GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
    TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
    GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
    AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
    GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
    ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
    CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
    ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
    GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
    TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
    CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
    TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
    AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
    AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
    ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
    CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
    AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
    ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
    GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
    GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
    AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326408.1
    CDS110..6022
    Translation

    Target ORF information:

    RefSeq Version XM_021470733.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
    ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
    TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
    AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
    GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
    AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
    GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
    GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
    GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
    CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
    ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
    GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
    TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
    GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
    CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
    TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
    TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
    TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
    GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
    CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
    CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
    CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
    AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
    ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
    ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
    GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
    TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
    GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
    AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
    GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
    ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
    CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
    ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
    GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
    TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
    CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
    TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
    AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
    AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
    ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
    CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
    AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
    ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
    GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
    GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
    AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_009298137.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296412.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X16, mRNA.
    Price & Availability↑
    CloneID ODa44627
    Clone ID Related Accession (Same CDS sequence) XM_009298137.3 , XM_009298137.2
    Accession Version XM_009298137.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5817bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298137.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
    TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
    GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
    CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
    CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
    ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
    CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
    GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
    TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
    CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
    TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
    GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
    GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
    CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
    GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
    CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
    GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
    AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
    AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
    GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
    GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
    AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
    CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
    GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
    TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
    TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
    AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
    ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
    ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
    GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
    ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
    CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
    TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
    TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
    ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
    GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
    AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
    AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
    AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
    GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
    TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
    CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
    CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
    TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
    CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
    TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
    CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
    CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
    CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
    CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
    ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
    GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
    CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
    AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
    GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
    TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
    CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
    GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
    CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
    CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296412.1
    CDS110..5926
    Translation

    Target ORF information:

    RefSeq Version XM_009298137.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298137.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
    TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
    GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
    CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
    CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
    ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
    CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
    GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
    TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
    CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
    TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
    GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
    GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
    CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
    GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
    CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
    GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
    AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
    AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
    GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
    GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
    AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
    CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
    GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
    TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
    TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
    AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
    ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
    ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
    GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
    ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
    CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
    TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
    TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
    ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
    GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
    AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
    AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
    AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
    GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
    TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
    CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
    CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
    TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
    CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
    TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
    CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
    CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
    CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
    CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
    ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
    GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
    CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
    AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
    GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
    TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
    CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
    GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
    CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
    CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_009298137.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296412.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA.
    Price & Availability↑
    CloneID ODa44627
    Clone ID Related Accession (Same CDS sequence) XM_009298137.3 , XM_009298137.2
    Accession Version XM_009298137.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5817bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
    TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
    GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
    CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
    CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
    ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
    CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
    GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
    TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
    CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
    TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
    GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
    GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
    CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
    GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
    CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
    GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
    AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
    AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
    GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
    GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
    AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
    CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
    GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
    TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
    TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
    AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
    ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
    ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
    GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
    ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
    CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
    TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
    TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
    ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
    GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
    AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
    AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
    AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
    GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
    TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
    CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
    CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
    TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
    CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
    TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
    CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
    CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
    CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
    CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
    ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
    GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
    CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
    AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
    GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
    TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
    CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
    GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
    CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
    CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296412.1
    CDS115..5931
    Translation

    Target ORF information:

    RefSeq Version XM_009298137.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298137.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
    TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
    GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
    CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
    CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
    ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
    CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
    GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
    TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
    CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
    TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
    GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
    GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
    CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
    GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
    CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
    GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
    AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
    AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
    GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
    GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
    AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
    CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
    GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
    TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
    TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
    AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
    ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
    ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
    GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
    ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
    CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
    TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
    TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
    ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
    GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
    AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
    AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
    AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
    GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
    TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
    CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
    CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
    TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
    CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
    TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
    CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
    CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
    CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
    CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
    ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
    GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
    CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
    AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
    GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
    TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
    CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
    GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
    CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
    CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470734.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326409.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X17, mRNA.
    Price & Availability↑
    CloneID ODa44628
    Clone ID Related Accession (Same CDS sequence) XM_021470734.1
    Accession Version XM_021470734.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGAATT ACCAGAAATA CATTACTGTT ATGCAGCTGG CTCTAGGGGT GACTGCCTAC 
    AACAGAGACC GTAGCCTGCC TCCCAGAAAA CATATGTGGG TCTTCCCCAA ACACGACAAA 
    GACAAATACG GCCCTGCAGT TTATTATCCG AAGTTCAACG CCTACATCCC CAACCCAGAC 
    AAAGTCTCTG TGCTGGATGA ATCTGAGGGA TCGGGCCACG GCAAACCATC CCTGCGCAGA 
    ATCAAGGGCC GAATCCACCG CAGCAAGAGC CTGGACAGCA TCGACCTGCT GGACTCCAAC 
    AGTGCAGCAA TGGATGAGAC GGTAATATGG GAACAGCACA CTGTAACTCT ACACAGGGCT 
    CCAGGGTTTG GCTTCGGGAT CGCCATCTCC GGCGGACGGG ACAACCCTCA CTTCCAGAGC 
    GGGGAGACGT CCATCGTCAT ATCAGATGTG CTGAAGGGAG GCCCCGCCGA GGGCCTGCTG 
    CAGGAGAATG ACAGAGTGGT GATGGTCAAC GCCGTCTCCA TGGACAATGT GGATCACGCT 
    TACGCAGTGC AGCAGCTCCG CAAAAGTGGC AAGAATGCAA AAATAACCAT CAGGAGAAAG 
    CGTAAGGTGC AGATCCCGTC AGGCCGGCCG GGTGAACGCG AGACTATGTC AGAGCATGAC 
    GAGGACGACG ACAGCTACGA GGATGAGATC TACGAGTCCC GGAGCGCGCG AAGCGGACCC 
    AGCGCCTACA GTGGAGGTGG AGGCGGCGGC GGAGGTGGAG GAGCCTCGGG ACGGAGGAGC 
    GGCAGAGGCG ACAGGCTGAG TGGGAGGAGG GAGCGGGACA GAGAGCGCAG CGGCTCCAGA 
    GAGAGGAGTC TGTCCCCGCG CTCGGACCGC CACTCTGTGT CCTCCAACCT CCCCCCACGG 
    CCAGCCAAAG TCACACTCGT CAAATCCCGC AAGAATGAAG CATGTATGGC GTCAGGAAGT 
    TCTCAGAAGG TGACATCAGC AGAATACGGG CTTCGCCTGG CCAGCCACAT CTTCGTGAAG 
    GACATTTCCC CCGAGAGCCT GGCAGCCCGA GACGGAAACA TCCAAGAGGG AGACGTGGTG 
    CTTAAGATTA ATGGCACGGT AACAGAGAAT TTGTCCCTGA TCGATGCTAA GAAGCTGATC 
    GAGAGGTCAA AGGGAAAGCT GAAGATGGTG GTGCAGAGAG ACGAGCGGGC GACGCTGCTG 
    AACATCCCCG ATCTGGACGA CAGCATACCT TCTGCTAATG CCTCGGACAG AGATGATATT 
    TCAGACATCC ACTCCGTGGC CTCTGATCAT TCCAACCGAT CCCACGACCG GAAGAGGAGC 
    AGCCGCTCTC GTTCTCCAGA CCGCCGCTCC GAACCCTCAG ACCACTCCAG ACACTCTCCA 
    CCACAGATCA GCAACGGCAG GGCCACAGGG CCGACGGCTC AGAGAACGCG GCCAGTTCAC 
    AGGAGCCGTG ATGAAGACAG GATCTCCAAG GCCGGGCCGC TTCCTCTACC TGCTAAGATG 
    GTGGAGGAGA TGCCGAAAGA GCAGAGCGCA GCCAGAGAAA CAGAGAAACA GCTCCCCCCT 
    CTGCCAGAGC CCAAACCTGT GTACGCTCAG CCGGGCCAGC CGGACGTGGA CCTGCCTGTT 
    AGTCCGGCAG ATGCTCCTGT GCCCAGCGTC ACACATGATG ACAGCATCTT ACGGCCGAGC 
    ATGAAGCTGG TGAAGTTCAA GAAGGGCGAG AGCGTGGGCT TGAGGCTCGC TGGGGGGAAC 
    GACGTTGGGA TATTTGTGGC GGGAGTTCTG GAGGACAGTC CTGCTGCTAA AGAGGGCCTG 
    GAAGAGGGAG ACCAGATTCT CAGGGTAAAT AATGTGGATT TTGCAAATAT CATCCGAGAG 
    GAGGCAGTGC TGTTCCTGCT GGATCTGCCC AAAGGAGAGG AGGTCACCAT CCTGGCCCAG 
    AAGAAGAAAG ACGTGTACCG CCGCATTGTA GAGTCTGACG TGGGAGACTC GTTCTACATC 
    AGGACTCATT TTGAATATGA GAAGGAGTCG CCCTATGGCC TGAGCTTCAA TAAGGGGGAG 
    GTCTTCAGAG TCGTCGACAC CCTTTACAAT GGCAAACTGG GCTCCTGGCT CGCCATCCGC 
    ATCGGCAAAA ACCACCAGGA GGTGGAGCGG GGCATCATCC CCAACAAGAA CAGGGCTGAG 
    CAGTTATCAA GTGTCCAGTA CACTCTCCCA AAGACTCCAG GAGGGGATCG CGCCGACTTC 
    TGGAGATTCA GAGGCCTCCG CAGCTCCAAA CGAAACCTGA GGAAGAGCAG AGAAGACCTG 
    TCTGCTCAGC CGGTCCAAAC CAAATTCCCT GCTTATGAAA GAGTGGTGCT TAGAGAAGCT 
    GGTTTCTTGA GGCCAGTGGT CATTTTCGGA CCGATCGCGG ATGTGGCTAG AGAGAAGCTG 
    TCCAGAGAGG AGACTGACCT CTTCGAACTT GCAAAGAGTG AACCCAGAGA TGCGGGCACA 
    GACCAGCGCA GCTCCGGAAT CATCCGTCTC CATACCATTA AACAGATCAT TGACCGAGAC 
    AAGCACGCGG TGCTGGACAT CACCCCTAAT GCGGTGGACC GTCTGAACTA CGCTCAGTGG 
    TACCCCATCG TGGTGTTTCT GAATCCAGAC AGCAAACAGG GCGTCAAGAA CATGAGGACC 
    AGGCTCTGTC CCGAGTCCAG GAAGAGCGCC CGAAAGCTCT ACGAGCGTGC TCTCAAACTG 
    AGGAAGAACA ACCATCACCT TTTCACCACC ACTATTAACT TGAACAACAT GAATGACGGG 
    TGGTACGGTG CTTTGAAAGA GAGCATCCAG CAGCAGCAGA ACCAGTTGGT CTGGGTCTCA 
    GAGGGCAAGG CCGACGGCGC TCCTGACGAT GACCTTGACA TCCATGACGA CCGCCTCTCC 
    TACCTCTCGG CTCCGGGCAG CGAGTACAGC ATGTACAGCA CCGACAGCCG CCACACGTCC 
    GACTATGAGG ACACCGACAC GGAGGGTGGT GCCTACACCG ACCAGGAGCT GGACGAGACC 
    ATGAACGATG AGGTGGGCCT GCCTAGCGAG CCTGCCATCA CACGCTCATC TGAGCCTGTG 
    CGAGAGGACC CGCCAGTCAT TCAGGACGCA GCTGGATACC CCAGCTACCA GCATGCGGTA 
    CCGCAACCTG AAGCGGTGAG CCGCATTGAC CCGGCCGGGT TTAAGATGGC AGCGCCGCAG 
    CAGCAAGCTG AGGCTGCTCT GGCCTCGCCG CCCCCTCCCT CTGCAGCGGT CCAGCCCACC 
    GCAACACTAG CGGGTACGAA CCCCGAGGAG CCGCCTGGCA CCCCTCAGGC CGACTCCCTT 
    AACAGCCCCG TCCCTGTTCC CGACCCCCAG CCCGAACCTG AGCTCGCTCA GCCCCCAACA 
    CACGACCCCC ACCAGTCGCT TCCTCCTGGC CCAGATCCAA AGATGTATAA AAAGGATCTG 
    TACAGCATGG ACGAGCCGGT GCGAGTGACC CACGGCGTGA AGCCTCCCCC TCCCCAGCAG 
    CAGCAGCAGC CTTTAGGCCC CCCAACCTCG CTCTCCTACA GCCACCAGCC TGTCTACCAG 
    GACCAACAGC CATACCGCCA GTACGACCAC CCGCCTTATG GCTATGACGG TGGCGGCTAC 
    GCACAACCAA AGCCTCACAA CTACGACCCG CACCTGCACT ACGACAACCG TGTGCCTCAC 
    TATAACGAGC AGTGGCCCAC GTACGACCAG CAGCAGCAGC AGCAGACTTC CCCCCAGCCG 
    CCACCTGCGT CCGGCTTCCC TCAGGGCCAC CAGCCTCCGC CTCCACCACC GATGGGATAT 
    GAGCCACGCT CCCCATACGA GGACGGACCT AACAGGGATT TCAGCCCTCC TCAGTCCCAG 
    TATGACGGTG TGTCCCCAAT GGGCTACGAT CACCGGCCGC GGCACCCCAA ACCTGCACCG 
    GTCCGATATG AAGAGCCTCC ACCTCCACCT CCTCCGGTGT CCTACGATGC ACGGTCACCT 
    TTCGAACCCG AGCCGCATGC TTTCCCAGGC AACACACATC GCTCGCCAGA CCCACCGAAA 
    CAGTATTATG GAGACGCCGC TATGAGGCCC TTGTACAACC CTGGACCTCC GAACAGAGTG 
    TACAAACATG AGCCGGTGAA CTCTGAGCCT CCTGTACCGC CTCCCAAACC TGAATCTGTC 
    CTGTCTCCAG GAGAACCGCC CCATTCTGCA GCTTCCAAAC CATTGCCGCC TCCTCCCAGA 
    GATGATGACG ATGATGATGA CGATGATGAT GACCCTGCCA TGAAGCCCCA GTCTGTGCTC 
    AACAGGGTCA AAATGTTCGA AAACAAACGC TCTGTGTCGG TAGACCGAGC CAAGGACGCA 
    CCTGACGTCA CTGGAATTAG GCCCACAGAT CTCCCCAAAC CTGCGAGCAC CCCTGGACCT 
    GTGCTCAAAG CCAACTCTCT CAGCAACCTG GAGCAGGACA AACCCTCCTA CAGAGCTCCA 
    GAGCCGCAGA AACCTCAGAC GCGTGTTGGG GACGATGTGG TTCGCTCCAA CCACTACGAC 
    CCTGATGATG ATGAAGAGTA CTACAGAAAA CAGCTCTCAT TTTTCGACCG CCGCAGTTTC 
    GACAGTAAGG TCATGAATCC ACCCAGCGCT GGAGTAAACC GCTTCCATGA GCCGCCCAAA 
    CCACCCCAGA CACAGATCGC TTATCCTTTC GCTAGGACGG AGTCTGTGGA GAAGGTGAGT 
    CCTGTGGACA AGAGATACGA GCCTCTGCCG CCAGTCAACC CTTCACCTGC TCCATACAGC 
    CAGAACACAC CTGTCGCTCC GCCCACATCC CTGCCCAAAC TCACCAACAT CGAGGTGAAC 
    TCTCTGCCAG ATCCTCACAG CTCTCCCAAA GCCAAACCGG ACCTGTCTGC TCTGAGAGCT 
    CCGACCCGGG ACGAGCCCAT CCAGAGCAGC TACTTGCCCC CCAAGTCCAC CATCAATGGC 
    ATGGATGCTC CTCCCAAAAC ACTCGGCGTG CCGACCAGCT ACAACCGCTA CGTCCCCAAG 
    CCTTACACCA GCTCGGCGCG ACCCTTCGAG CGCAAGTTCG AGAGTCCGAA ATTCAACCAC 
    AACCTGCTTC CCAACGACAC GCAGTCCAAA CCCAGTGTCA ACAACAACCT GAAGCCTCAG 
    ATCTCCCCAC AACCCCAGGA CACGGACAGC GGCGTCGACA CCTTCACTCG CACCATGGAC 
    AACAGGCCAA AATATCAGCA CAACAACATC AACGCCATTC CCAAAGCCGT CCCAGTCAGC 
    CCCAGTGCAC TGGATGATGA TGAAGAAGAT GAGGGACACA CAGTTGTGGC CACGGCGCGG 
    GGCATCTTTA ACTGTAATGG CGGGGTGTTG AGCTCTATAG AGACGGGCGT CAGCATCATT 
    ATCCCACAGG GAGCCATTCC TGATAGTGTG GAGCAGGAGA TCTACTTCAA AGTGTGCCGG 
    GACAACAGCA TCCTGCCTCC TCTGGATAAG GAGAAAGGTG AAACTCTGCT GAGTCCGCTG 
    GTGATGTGTG GTCCTCACGG CCTGAAGTTC CTGAAGCCTG TGGAGCTGCG CTTGCCACAC 
    TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCCGA CTCCTCGTCG 
    GGTGACCCCA AATGCTGGCA AAACAAGTGT CTACCAGGAG ACCCCAATTA CCTGGTGGGC 
    GCAAACTGTG TGTCTGTGCT GATCGACCAC TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326409.1
    CDS427..6102
    Translation

    Target ORF information:

    RefSeq Version XM_021470734.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470734.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGAATT ACCAGAAATA CATTACTGTT ATGCAGCTGG CTCTAGGGGT GACTGCCTAC 
    AACAGAGACC GTAGCCTGCC TCCCAGAAAA CATATGTGGG TCTTCCCCAA ACACGACAAA 
    GACAAATACG GCCCTGCAGT TTATTATCCG AAGTTCAACG CCTACATCCC CAACCCAGAC 
    AAAGTCTCTG TGCTGGATGA ATCTGAGGGA TCGGGCCACG GCAAACCATC CCTGCGCAGA 
    ATCAAGGGCC GAATCCACCG CAGCAAGAGC CTGGACAGCA TCGACCTGCT GGACTCCAAC 
    AGTGCAGCAA TGGATGAGAC GGTAATATGG GAACAGCACA CTGTAACTCT ACACAGGGCT 
    CCAGGGTTTG GCTTCGGGAT CGCCATCTCC GGCGGACGGG ACAACCCTCA CTTCCAGAGC 
    GGGGAGACGT CCATCGTCAT ATCAGATGTG CTGAAGGGAG GCCCCGCCGA GGGCCTGCTG 
    CAGGAGAATG ACAGAGTGGT GATGGTCAAC GCCGTCTCCA TGGACAATGT GGATCACGCT 
    TACGCAGTGC AGCAGCTCCG CAAAAGTGGC AAGAATGCAA AAATAACCAT CAGGAGAAAG 
    CGTAAGGTGC AGATCCCGTC AGGCCGGCCG GGTGAACGCG AGACTATGTC AGAGCATGAC 
    GAGGACGACG ACAGCTACGA GGATGAGATC TACGAGTCCC GGAGCGCGCG AAGCGGACCC 
    AGCGCCTACA GTGGAGGTGG AGGCGGCGGC GGAGGTGGAG GAGCCTCGGG ACGGAGGAGC 
    GGCAGAGGCG ACAGGCTGAG TGGGAGGAGG GAGCGGGACA GAGAGCGCAG CGGCTCCAGA 
    GAGAGGAGTC TGTCCCCGCG CTCGGACCGC CACTCTGTGT CCTCCAACCT CCCCCCACGG 
    CCAGCCAAAG TCACACTCGT CAAATCCCGC AAGAATGAAG CATGTATGGC GTCAGGAAGT 
    TCTCAGAAGG TGACATCAGC AGAATACGGG CTTCGCCTGG CCAGCCACAT CTTCGTGAAG 
    GACATTTCCC CCGAGAGCCT GGCAGCCCGA GACGGAAACA TCCAAGAGGG AGACGTGGTG 
    CTTAAGATTA ATGGCACGGT AACAGAGAAT TTGTCCCTGA TCGATGCTAA GAAGCTGATC 
    GAGAGGTCAA AGGGAAAGCT GAAGATGGTG GTGCAGAGAG ACGAGCGGGC GACGCTGCTG 
    AACATCCCCG ATCTGGACGA CAGCATACCT TCTGCTAATG CCTCGGACAG AGATGATATT 
    TCAGACATCC ACTCCGTGGC CTCTGATCAT TCCAACCGAT CCCACGACCG GAAGAGGAGC 
    AGCCGCTCTC GTTCTCCAGA CCGCCGCTCC GAACCCTCAG ACCACTCCAG ACACTCTCCA 
    CCACAGATCA GCAACGGCAG GGCCACAGGG CCGACGGCTC AGAGAACGCG GCCAGTTCAC 
    AGGAGCCGTG ATGAAGACAG GATCTCCAAG GCCGGGCCGC TTCCTCTACC TGCTAAGATG 
    GTGGAGGAGA TGCCGAAAGA GCAGAGCGCA GCCAGAGAAA CAGAGAAACA GCTCCCCCCT 
    CTGCCAGAGC CCAAACCTGT GTACGCTCAG CCGGGCCAGC CGGACGTGGA CCTGCCTGTT 
    AGTCCGGCAG ATGCTCCTGT GCCCAGCGTC ACACATGATG ACAGCATCTT ACGGCCGAGC 
    ATGAAGCTGG TGAAGTTCAA GAAGGGCGAG AGCGTGGGCT TGAGGCTCGC TGGGGGGAAC 
    GACGTTGGGA TATTTGTGGC GGGAGTTCTG GAGGACAGTC CTGCTGCTAA AGAGGGCCTG 
    GAAGAGGGAG ACCAGATTCT CAGGGTAAAT AATGTGGATT TTGCAAATAT CATCCGAGAG 
    GAGGCAGTGC TGTTCCTGCT GGATCTGCCC AAAGGAGAGG AGGTCACCAT CCTGGCCCAG 
    AAGAAGAAAG ACGTGTACCG CCGCATTGTA GAGTCTGACG TGGGAGACTC GTTCTACATC 
    AGGACTCATT TTGAATATGA GAAGGAGTCG CCCTATGGCC TGAGCTTCAA TAAGGGGGAG 
    GTCTTCAGAG TCGTCGACAC CCTTTACAAT GGCAAACTGG GCTCCTGGCT CGCCATCCGC 
    ATCGGCAAAA ACCACCAGGA GGTGGAGCGG GGCATCATCC CCAACAAGAA CAGGGCTGAG 
    CAGTTATCAA GTGTCCAGTA CACTCTCCCA AAGACTCCAG GAGGGGATCG CGCCGACTTC 
    TGGAGATTCA GAGGCCTCCG CAGCTCCAAA CGAAACCTGA GGAAGAGCAG AGAAGACCTG 
    TCTGCTCAGC CGGTCCAAAC CAAATTCCCT GCTTATGAAA GAGTGGTGCT TAGAGAAGCT 
    GGTTTCTTGA GGCCAGTGGT CATTTTCGGA CCGATCGCGG ATGTGGCTAG AGAGAAGCTG 
    TCCAGAGAGG AGACTGACCT CTTCGAACTT GCAAAGAGTG AACCCAGAGA TGCGGGCACA 
    GACCAGCGCA GCTCCGGAAT CATCCGTCTC CATACCATTA AACAGATCAT TGACCGAGAC 
    AAGCACGCGG TGCTGGACAT CACCCCTAAT GCGGTGGACC GTCTGAACTA CGCTCAGTGG 
    TACCCCATCG TGGTGTTTCT GAATCCAGAC AGCAAACAGG GCGTCAAGAA CATGAGGACC 
    AGGCTCTGTC CCGAGTCCAG GAAGAGCGCC CGAAAGCTCT ACGAGCGTGC TCTCAAACTG 
    AGGAAGAACA ACCATCACCT TTTCACCACC ACTATTAACT TGAACAACAT GAATGACGGG 
    TGGTACGGTG CTTTGAAAGA GAGCATCCAG CAGCAGCAGA ACCAGTTGGT CTGGGTCTCA 
    GAGGGCAAGG CCGACGGCGC TCCTGACGAT GACCTTGACA TCCATGACGA CCGCCTCTCC 
    TACCTCTCGG CTCCGGGCAG CGAGTACAGC ATGTACAGCA CCGACAGCCG CCACACGTCC 
    GACTATGAGG ACACCGACAC GGAGGGTGGT GCCTACACCG ACCAGGAGCT GGACGAGACC 
    ATGAACGATG AGGTGGGCCT GCCTAGCGAG CCTGCCATCA CACGCTCATC TGAGCCTGTG 
    CGAGAGGACC CGCCAGTCAT TCAGGACGCA GCTGGATACC CCAGCTACCA GCATGCGGTA 
    CCGCAACCTG AAGCGGTGAG CCGCATTGAC CCGGCCGGGT TTAAGATGGC AGCGCCGCAG 
    CAGCAAGCTG AGGCTGCTCT GGCCTCGCCG CCCCCTCCCT CTGCAGCGGT CCAGCCCACC 
    GCAACACTAG CGGGTACGAA CCCCGAGGAG CCGCCTGGCA CCCCTCAGGC CGACTCCCTT 
    AACAGCCCCG TCCCTGTTCC CGACCCCCAG CCCGAACCTG AGCTCGCTCA GCCCCCAACA 
    CACGACCCCC ACCAGTCGCT TCCTCCTGGC CCAGATCCAA AGATGTATAA AAAGGATCTG 
    TACAGCATGG ACGAGCCGGT GCGAGTGACC CACGGCGTGA AGCCTCCCCC TCCCCAGCAG 
    CAGCAGCAGC CTTTAGGCCC CCCAACCTCG CTCTCCTACA GCCACCAGCC TGTCTACCAG 
    GACCAACAGC CATACCGCCA GTACGACCAC CCGCCTTATG GCTATGACGG TGGCGGCTAC 
    GCACAACCAA AGCCTCACAA CTACGACCCG CACCTGCACT ACGACAACCG TGTGCCTCAC 
    TATAACGAGC AGTGGCCCAC GTACGACCAG CAGCAGCAGC AGCAGACTTC CCCCCAGCCG 
    CCACCTGCGT CCGGCTTCCC TCAGGGCCAC CAGCCTCCGC CTCCACCACC GATGGGATAT 
    GAGCCACGCT CCCCATACGA GGACGGACCT AACAGGGATT TCAGCCCTCC TCAGTCCCAG 
    TATGACGGTG TGTCCCCAAT GGGCTACGAT CACCGGCCGC GGCACCCCAA ACCTGCACCG 
    GTCCGATATG AAGAGCCTCC ACCTCCACCT CCTCCGGTGT CCTACGATGC ACGGTCACCT 
    TTCGAACCCG AGCCGCATGC TTTCCCAGGC AACACACATC GCTCGCCAGA CCCACCGAAA 
    CAGTATTATG GAGACGCCGC TATGAGGCCC TTGTACAACC CTGGACCTCC GAACAGAGTG 
    TACAAACATG AGCCGGTGAA CTCTGAGCCT CCTGTACCGC CTCCCAAACC TGAATCTGTC 
    CTGTCTCCAG GAGAACCGCC CCATTCTGCA GCTTCCAAAC CATTGCCGCC TCCTCCCAGA 
    GATGATGACG ATGATGATGA CGATGATGAT GACCCTGCCA TGAAGCCCCA GTCTGTGCTC 
    AACAGGGTCA AAATGTTCGA AAACAAACGC TCTGTGTCGG TAGACCGAGC CAAGGACGCA 
    CCTGACGTCA CTGGAATTAG GCCCACAGAT CTCCCCAAAC CTGCGAGCAC CCCTGGACCT 
    GTGCTCAAAG CCAACTCTCT CAGCAACCTG GAGCAGGACA AACCCTCCTA CAGAGCTCCA 
    GAGCCGCAGA AACCTCAGAC GCGTGTTGGG GACGATGTGG TTCGCTCCAA CCACTACGAC 
    CCTGATGATG ATGAAGAGTA CTACAGAAAA CAGCTCTCAT TTTTCGACCG CCGCAGTTTC 
    GACAGTAAGG TCATGAATCC ACCCAGCGCT GGAGTAAACC GCTTCCATGA GCCGCCCAAA 
    CCACCCCAGA CACAGATCGC TTATCCTTTC GCTAGGACGG AGTCTGTGGA GAAGGTGAGT 
    CCTGTGGACA AGAGATACGA GCCTCTGCCG CCAGTCAACC CTTCACCTGC TCCATACAGC 
    CAGAACACAC CTGTCGCTCC GCCCACATCC CTGCCCAAAC TCACCAACAT CGAGGTGAAC 
    TCTCTGCCAG ATCCTCACAG CTCTCCCAAA GCCAAACCGG ACCTGTCTGC TCTGAGAGCT 
    CCGACCCGGG ACGAGCCCAT CCAGAGCAGC TACTTGCCCC CCAAGTCCAC CATCAATGGC 
    ATGGATGCTC CTCCCAAAAC ACTCGGCGTG CCGACCAGCT ACAACCGCTA CGTCCCCAAG 
    CCTTACACCA GCTCGGCGCG ACCCTTCGAG CGCAAGTTCG AGAGTCCGAA ATTCAACCAC 
    AACCTGCTTC CCAACGACAC GCAGTCCAAA CCCAGTGTCA ACAACAACCT GAAGCCTCAG 
    ATCTCCCCAC AACCCCAGGA CACGGACAGC GGCGTCGACA CCTTCACTCG CACCATGGAC 
    AACAGGCCAA AATATCAGCA CAACAACATC AACGCCATTC CCAAAGCCGT CCCAGTCAGC 
    CCCAGTGCAC TGGATGATGA TGAAGAAGAT GAGGGACACA CAGTTGTGGC CACGGCGCGG 
    GGCATCTTTA ACTGTAATGG CGGGGTGTTG AGCTCTATAG AGACGGGCGT CAGCATCATT 
    ATCCCACAGG GAGCCATTCC TGATAGTGTG GAGCAGGAGA TCTACTTCAA AGTGTGCCGG 
    GACAACAGCA TCCTGCCTCC TCTGGATAAG GAGAAAGGTG AAACTCTGCT GAGTCCGCTG 
    GTGATGTGTG GTCCTCACGG CCTGAAGTTC CTGAAGCCTG TGGAGCTGCG CTTGCCACAC 
    TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCCGA CTCCTCGTCG 
    GGTGACCCCA AATGCTGGCA AAACAAGTGT CTACCAGGAG ACCCCAATTA CCTGGTGGGC 
    GCAAACTGTG TGTCTGTGCT GATCGACCAC TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470736.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326411.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X18, mRNA.
    Price & Availability↑
    CloneID ODa44629
    Clone ID Related Accession (Same CDS sequence) XM_021470736.1
    Accession Version XM_021470736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCTGCCC GGGCTGCCTC TAATAAGAGT GCAGCAATGG ATGAGACGGT AATATGGGAA 
    CAGCACACTG TAACTCTACA CAGGGCTCCA GGGTTTGGCT TCGGGATCGC CATCTCCGGC 
    GGACGGGACA ACCCTCACTT CCAGAGCGGG GAGACGTCCA TCGTCATATC AGATGTGCTG 
    AAGGGAGGCC CCGCCGAGGG CCTGCTGCAG GAGAATGACA GAGTGGTGAT GGTCAACGCC 
    GTCTCCATGG ACAATGTGGA TCACGCTTAC GCAGTGCAGC AGCTCCGCAA AAGTGGCAAG 
    AATGCAAAAA TAACCATCAG GAGAAAGCGT AAGGTGCAGA TCCCGTCAGG CCGGCCGGGT 
    GAACGCGAGA CTATGTCAGA GCATGACGAG GACGACGACA GCTACGAGGA TGAGATCTAC 
    GAGTCCCGGA GCGCGCGAAG CGGACCCAGC GCCTACAGTG GAGGTGGAGG CGGCGGCGGA 
    GGTGGAGGAG CCTCGGGACG GAGGAGCGGC AGAGGCGACA GGCTGAGTGG GAGGAGGGAG 
    CGGGACAGAG AGCGCAGCGG CTCCAGAGAG AGGAGTCTGT CCCCGCGCTC GGACCGCCAC 
    TCTGTGTCCT CCAACCTCCC CCCACGGCCA GCCAAAGTCA CACTCGTCAA ATCCCGCAAG 
    AATGAAGCAT GTATGGCGTC AGGAAGTTCT CAGAAGGTGA CATCAGCAGA ATACGGGCTT 
    CGCCTGGCCA GCCACATCTT CGTGAAGGAC ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC 
    GGAAACATCC AAGAGGGAGA CGTGGTGCTT AAGATTAATG GCACGGTAAC AGAGAATTTG 
    TCCCTGATCG ATGCTAAGAA GCTGATCGAG AGGTCAAAGG GAAAGCTGAA GATGGTGGTG 
    CAGAGAGACG AGCGGGCGAC GCTGCTGAAC ATCCCCGATC TGGACGACAG CATACCTTCT 
    GCTAATGCCT CGGACAGAGA TGATATTTCA GACATCCACT CCGTGGCCTC TGATCATTCC 
    AACCGATCCC ACGACCGGAA GAGGAGCAGC CGCTCTCGTT CTCCAGACCG CCGCTCCGAA 
    CCCTCAGACC ACTCCAGACA CTCTCCACCA CAGATCAGCA ACGGCAGGGC CACAGGGCCG 
    ACGGCTCAGA GAACGCGGCC AGTTCACAGG AGCCGTGATG AAGACAGGAT CTCCAAGGCC 
    GGGCCGCTTC CTCTACCTGC TAAGATGGTG GAGGAGATGC CGAAAGAGCA GAGCGCAGCC 
    AGAGAAACAG AGAAACAGCT CCCCCCTCTG CCAGAGCCCA AACCTGTGTA CGCTCAGCCG 
    GGCCAGCCGG ACGTGGACCT GCCTGTTAGT CCGGCAGATG CTCCTGTGCC CAGCGTCACA 
    CATGATGACA GCATCTTACG GCCGAGCATG AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC 
    GTGGGCTTGA GGCTCGCTGG GGGGAACGAC GTTGGGATAT TTGTGGCGGG AGTTCTGGAG 
    GACAGTCCTG CTGCTAAAGA GGGCCTGGAA GAGGGAGACC AGATTCTCAG GGTAAATAAT 
    GTGGATTTTG CAAATATCAT CCGAGAGGAG GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA 
    GGAGAGGAGG TCACCATCCT GGCCCAGAAG AAGAAAGACG TGTACCGCCG CATTGTAGAG 
    TCTGACGTGG GAGACTCGTT CTACATCAGG ACTCATTTTG AATATGAGAA GGAGTCGCCC 
    TATGGCCTGA GCTTCAATAA GGGGGAGGTC TTCAGAGTCG TCGACACCCT TTACAATGGC 
    AAACTGGGCT CCTGGCTCGC CATCCGCATC GGCAAAAACC ACCAGGAGGT GGAGCGGGGC 
    ATCATCCCCA ACAAGAACAG GGCTGAGCAG TTATCAAGTG TCCAGTACAC TCTCCCAAAG 
    ACTCCAGGAG GGGATCGCGC CGACTTCTGG AGATTCAGAG GCCTCCGCAG CTCCAAACGA 
    AACCTGAGGA AGAGCAGAGA AGACCTGTCT GCTCAGCCGG TCCAAACCAA ATTCCCTGCT 
    TATGAAAGAG TGGTGCTTAG AGAAGCTGGT TTCTTGAGGC CAGTGGTCAT TTTCGGACCG 
    ATCGCGGATG TGGCTAGAGA GAAGCTGTCC AGAGAGGAGA CTGACCTCTT CGAACTTGCA 
    AAGAGTGAAC CCAGAGATGC GGGCACAGAC CAGCGCAGCT CCGGAATCAT CCGTCTCCAT 
    ACCATTAAAC AGATCATTGA CCGAGACAAG CACGCGGTGC TGGACATCAC CCCTAATGCG 
    GTGGACCGTC TGAACTACGC TCAGTGGTAC CCCATCGTGG TGTTTCTGAA TCCAGACAGC 
    AAACAGGGCG TCAAGAACAT GAGGACCAGG CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA 
    AAGCTCTACG AGCGTGCTCT CAAACTGAGG AAGAACAACC ATCACCTTTT CACCACCACT 
    ATTAACTTGA ACAACATGAA TGACGGGTGG TACGGTGCTT TGAAAGAGAG CATCCAGCAG 
    CAGCAGAACC AGTTGGTCTG GGTCTCAGAG GGCAAGGCCG ACGGCGCTCC TGACGATGAC 
    CTTGACATCC ATGACGACCG CCTCTCCTAC CTCTCGGCTC CGGGCAGCGA GTACAGCATG 
    TACAGCACCG ACAGCCGCCA CACGTCCGAC TATGAGGACA CCGACACGGA GGGTGGTGCC 
    TACACCGACC AGGAGCTGGA CGAGACCATG AACGATGAGG TGGGCCTGCC TAGCGAGCCT 
    GCCATCACAC GCTCATCTGA GCCTGTGCGA GAGGACCCGC CAGTCATTCA GGACGCAGCT 
    GGATACCCCA GCTACCAGCA TGCGGTACCG CAACCTGAAG CGGTGAGCCG CATTGACCCG 
    GCCGGGTTTA AGATGGCAGC GCCGCAGCAG CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC 
    CCTCCCTCTG CAGCGGTCCA GCCCACCGCA ACACTAGCGG GTACGAACCC CGAGGAGCCG 
    CCTGGCACCC CTCAGGCCGA CTCCCTTAAC AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC 
    GAACCTGAGC TCGCTCAGCC CCCAACACAC GACCCCCACC AGTCGCTTCC TCCTGGCCCA 
    GATCCAAAGA TGTATAAAAA GGATCTGTAC AGCATGGACG AGCCGGTGCG AGTGACCCAC 
    GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG CAGCAGCCTT TAGGCCCCCC AACCTCGCTC 
    TCCTACAGCC ACCAGCCTGT CTACCAGGAC CAACAGCCAT ACCGCCAGTA CGACCACCCG 
    CCTTATGGCT ATGACGGTGG CGGCTACGCA CAACCAAAGC CTCACAACTA CGACCCGCAC 
    CTGCACTACG ACAACCGTGT GCCTCACTAT AACGAGCAGT GGCCCACGTA CGACCAGCAG 
    CAGCAGCAGC AGACTTCCCC CCAGCCGCCA CCTGCGTCCG GCTTCCCTCA GGGCCACCAG 
    CCTCCGCCTC CACCACCGAT GGGATATGAG CCACGCTCCC CATACGAGGA CGGACCTAAC 
    AGGGATTTCA GCCCTCCTCA GTCCCAGTAT GACGGTGTGT CCCCAATGGG CTACGATCAC 
    CGGCCGCGGC ACCCCAAACC TGCACCGGTC CGATATGAAG AGCCTCCACC TCCACCTCCT 
    CCGGTGTCCT ACGATGCACG GTCACCTTTC GAACCCGAGC CGCATGCTTT CCCAGGCAAC 
    ACACATCGCT CGCCAGACCC ACCGAAACAG TATTATGGAG ACGCCGCTAT GAGGCCCTTG 
    TACAACCCTG GACCTCCGAA CAGAGTGTAC AAACATGAGC CGGTGAACTC TGAGCCTCCT 
    GTACCGCCTC CCAAACCTGA ATCTGTCCTG TCTCCAGGAG AACCGCCCCA TTCTGCAGCT 
    TCCAAACCAT TGCCGCCTCC TCCCAGAGAT GATGACGATG ATGATGACGA TGATGATGAC 
    CCTGCCATGA AGCCCCAGTC TGTGCTCAAC AGGGTCAAAA TGTTCGAAAA CAAACGCTCT 
    GTGTCGGTAG ACCGAGCCAA GGACGCACCT GACGTCACTG GAATTAGGCC CACAGATCTC 
    CCCAAACCTG CGAGCACCCC TGGACCTGTG CTCAAAGCCA ACTCTCTCAG CAACCTGGAG 
    CAGGACAAAC CCTCCTACAG AGCTCCAGAG CCGCAGAAAC CTCAGACGCG TGTTGGGGAC 
    GATGTGGTTC GCTCCAACCA CTACGACCCT GATGATGATG AAGAGTACTA CAGAAAACAG 
    CTCTCATTTT TCGACCGCCG CAGTTTCGAC AGTAAGGTCA TGAATCCACC CAGCGCTGGA 
    GTAAACCGCT TCCATGAGCC GCCCAAACCA CCCCAGACAC AGATCGCTTA TCCTTTCGCT 
    AGGACGGAGT CTGTGGAGAA GGTGAGTCCT GTGGACAAGA GATACGAGCC TCTGCCGCCA 
    GTCAACCCTT CACCTGCTCC ATACAGCCAG AACACACCTG TCGCTCCGCC CACATCCCTG 
    CCCAAACTCA CCAACATCGA GGTGAACTCT CTGCCAGATC CTCACAGCTC TCCCAAAGCC 
    AAACCGGACC TGTCTGCTCT GAGAGCTCCG ACCCGGGACG AGCCCATCCA GAGCAGCTAC 
    TTGCCCCCCA AGTCCACCAT CAATGGCATG GATGCTCCTC CCAAAACACT CGGCGTGCCG 
    ACCAGCTACA ACCGCTACGT CCCCAAGCCT TACACCAGCT CGGCGCGACC CTTCGAGCGC 
    AAGTTCGAGA GTCCGAAATT CAACCACAAC CTGCTTCCCA ACGACACGCA GTCCAAACCC 
    AGTGTCAACA ACAACCTGAA GCCTCAGATC TCCCCACAAC CCCAGGACAC GGACAGCGGC 
    GTCGACACCT TCACTCGCAC CATGGACAAC AGGCCAAAAT ATCAGCACAA CAACATCAAC 
    GCCATTCCCA AAGCCGTCCC AGTCAGCCCC AGTGCACTGG ATGATGATGA AGAAGATGAG 
    GGACACACAG TTGTGGCCAC GGCGCGGGGC ATCTTTAACT GTAATGGCGG GGTGTTGAGC 
    TCTATAGAGA CGGGCGTCAG CATCATTATC CCACAGGGAG CCATTCCTGA TAGTGTGGAG 
    CAGGAGATCT ACTTCAAAGT GTGCCGGGAC AACAGCATCC TGCCTCCTCT GGATAAGGAG 
    AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG ATGTGTGGTC CTCACGGCCT GAAGTTCCTG 
    AAGCCTGTGG AGCTGCGCTT GCCACACTGT GCGTCTATGA CCCCTGATGG TTGGTCTTTT 
    GCTCTAAAAT CCTCCGACTC CTCGTCGGGT GACCCCAAAT GCTGGCAAAA CAAGTGTCTA 
    CCAGGAGACC CCAATTACCT GGTGGGCGCA AACTGTGTGT CTGTGCTGAT CGACCACTTC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326411.1
    CDS503..5905
    Translation

    Target ORF information:

    RefSeq Version XM_021470736.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCTGCCC GGGCTGCCTC TAATAAGAGT GCAGCAATGG ATGAGACGGT AATATGGGAA 
    CAGCACACTG TAACTCTACA CAGGGCTCCA GGGTTTGGCT TCGGGATCGC CATCTCCGGC 
    GGACGGGACA ACCCTCACTT CCAGAGCGGG GAGACGTCCA TCGTCATATC AGATGTGCTG 
    AAGGGAGGCC CCGCCGAGGG CCTGCTGCAG GAGAATGACA GAGTGGTGAT GGTCAACGCC 
    GTCTCCATGG ACAATGTGGA TCACGCTTAC GCAGTGCAGC AGCTCCGCAA AAGTGGCAAG 
    AATGCAAAAA TAACCATCAG GAGAAAGCGT AAGGTGCAGA TCCCGTCAGG CCGGCCGGGT 
    GAACGCGAGA CTATGTCAGA GCATGACGAG GACGACGACA GCTACGAGGA TGAGATCTAC 
    GAGTCCCGGA GCGCGCGAAG CGGACCCAGC GCCTACAGTG GAGGTGGAGG CGGCGGCGGA 
    GGTGGAGGAG CCTCGGGACG GAGGAGCGGC AGAGGCGACA GGCTGAGTGG GAGGAGGGAG 
    CGGGACAGAG AGCGCAGCGG CTCCAGAGAG AGGAGTCTGT CCCCGCGCTC GGACCGCCAC 
    TCTGTGTCCT CCAACCTCCC CCCACGGCCA GCCAAAGTCA CACTCGTCAA ATCCCGCAAG 
    AATGAAGCAT GTATGGCGTC AGGAAGTTCT CAGAAGGTGA CATCAGCAGA ATACGGGCTT 
    CGCCTGGCCA GCCACATCTT CGTGAAGGAC ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC 
    GGAAACATCC AAGAGGGAGA CGTGGTGCTT AAGATTAATG GCACGGTAAC AGAGAATTTG 
    TCCCTGATCG ATGCTAAGAA GCTGATCGAG AGGTCAAAGG GAAAGCTGAA GATGGTGGTG 
    CAGAGAGACG AGCGGGCGAC GCTGCTGAAC ATCCCCGATC TGGACGACAG CATACCTTCT 
    GCTAATGCCT CGGACAGAGA TGATATTTCA GACATCCACT CCGTGGCCTC TGATCATTCC 
    AACCGATCCC ACGACCGGAA GAGGAGCAGC CGCTCTCGTT CTCCAGACCG CCGCTCCGAA 
    CCCTCAGACC ACTCCAGACA CTCTCCACCA CAGATCAGCA ACGGCAGGGC CACAGGGCCG 
    ACGGCTCAGA GAACGCGGCC AGTTCACAGG AGCCGTGATG AAGACAGGAT CTCCAAGGCC 
    GGGCCGCTTC CTCTACCTGC TAAGATGGTG GAGGAGATGC CGAAAGAGCA GAGCGCAGCC 
    AGAGAAACAG AGAAACAGCT CCCCCCTCTG CCAGAGCCCA AACCTGTGTA CGCTCAGCCG 
    GGCCAGCCGG ACGTGGACCT GCCTGTTAGT CCGGCAGATG CTCCTGTGCC CAGCGTCACA 
    CATGATGACA GCATCTTACG GCCGAGCATG AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC 
    GTGGGCTTGA GGCTCGCTGG GGGGAACGAC GTTGGGATAT TTGTGGCGGG AGTTCTGGAG 
    GACAGTCCTG CTGCTAAAGA GGGCCTGGAA GAGGGAGACC AGATTCTCAG GGTAAATAAT 
    GTGGATTTTG CAAATATCAT CCGAGAGGAG GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA 
    GGAGAGGAGG TCACCATCCT GGCCCAGAAG AAGAAAGACG TGTACCGCCG CATTGTAGAG 
    TCTGACGTGG GAGACTCGTT CTACATCAGG ACTCATTTTG AATATGAGAA GGAGTCGCCC 
    TATGGCCTGA GCTTCAATAA GGGGGAGGTC TTCAGAGTCG TCGACACCCT TTACAATGGC 
    AAACTGGGCT CCTGGCTCGC CATCCGCATC GGCAAAAACC ACCAGGAGGT GGAGCGGGGC 
    ATCATCCCCA ACAAGAACAG GGCTGAGCAG TTATCAAGTG TCCAGTACAC TCTCCCAAAG 
    ACTCCAGGAG GGGATCGCGC CGACTTCTGG AGATTCAGAG GCCTCCGCAG CTCCAAACGA 
    AACCTGAGGA AGAGCAGAGA AGACCTGTCT GCTCAGCCGG TCCAAACCAA ATTCCCTGCT 
    TATGAAAGAG TGGTGCTTAG AGAAGCTGGT TTCTTGAGGC CAGTGGTCAT TTTCGGACCG 
    ATCGCGGATG TGGCTAGAGA GAAGCTGTCC AGAGAGGAGA CTGACCTCTT CGAACTTGCA 
    AAGAGTGAAC CCAGAGATGC GGGCACAGAC CAGCGCAGCT CCGGAATCAT CCGTCTCCAT 
    ACCATTAAAC AGATCATTGA CCGAGACAAG CACGCGGTGC TGGACATCAC CCCTAATGCG 
    GTGGACCGTC TGAACTACGC TCAGTGGTAC CCCATCGTGG TGTTTCTGAA TCCAGACAGC 
    AAACAGGGCG TCAAGAACAT GAGGACCAGG CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA 
    AAGCTCTACG AGCGTGCTCT CAAACTGAGG AAGAACAACC ATCACCTTTT CACCACCACT 
    ATTAACTTGA ACAACATGAA TGACGGGTGG TACGGTGCTT TGAAAGAGAG CATCCAGCAG 
    CAGCAGAACC AGTTGGTCTG GGTCTCAGAG GGCAAGGCCG ACGGCGCTCC TGACGATGAC 
    CTTGACATCC ATGACGACCG CCTCTCCTAC CTCTCGGCTC CGGGCAGCGA GTACAGCATG 
    TACAGCACCG ACAGCCGCCA CACGTCCGAC TATGAGGACA CCGACACGGA GGGTGGTGCC 
    TACACCGACC AGGAGCTGGA CGAGACCATG AACGATGAGG TGGGCCTGCC TAGCGAGCCT 
    GCCATCACAC GCTCATCTGA GCCTGTGCGA GAGGACCCGC CAGTCATTCA GGACGCAGCT 
    GGATACCCCA GCTACCAGCA TGCGGTACCG CAACCTGAAG CGGTGAGCCG CATTGACCCG 
    GCCGGGTTTA AGATGGCAGC GCCGCAGCAG CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC 
    CCTCCCTCTG CAGCGGTCCA GCCCACCGCA ACACTAGCGG GTACGAACCC CGAGGAGCCG 
    CCTGGCACCC CTCAGGCCGA CTCCCTTAAC AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC 
    GAACCTGAGC TCGCTCAGCC CCCAACACAC GACCCCCACC AGTCGCTTCC TCCTGGCCCA 
    GATCCAAAGA TGTATAAAAA GGATCTGTAC AGCATGGACG AGCCGGTGCG AGTGACCCAC 
    GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG CAGCAGCCTT TAGGCCCCCC AACCTCGCTC 
    TCCTACAGCC ACCAGCCTGT CTACCAGGAC CAACAGCCAT ACCGCCAGTA CGACCACCCG 
    CCTTATGGCT ATGACGGTGG CGGCTACGCA CAACCAAAGC CTCACAACTA CGACCCGCAC 
    CTGCACTACG ACAACCGTGT GCCTCACTAT AACGAGCAGT GGCCCACGTA CGACCAGCAG 
    CAGCAGCAGC AGACTTCCCC CCAGCCGCCA CCTGCGTCCG GCTTCCCTCA GGGCCACCAG 
    CCTCCGCCTC CACCACCGAT GGGATATGAG CCACGCTCCC CATACGAGGA CGGACCTAAC 
    AGGGATTTCA GCCCTCCTCA GTCCCAGTAT GACGGTGTGT CCCCAATGGG CTACGATCAC 
    CGGCCGCGGC ACCCCAAACC TGCACCGGTC CGATATGAAG AGCCTCCACC TCCACCTCCT 
    CCGGTGTCCT ACGATGCACG GTCACCTTTC GAACCCGAGC CGCATGCTTT CCCAGGCAAC 
    ACACATCGCT CGCCAGACCC ACCGAAACAG TATTATGGAG ACGCCGCTAT GAGGCCCTTG 
    TACAACCCTG GACCTCCGAA CAGAGTGTAC AAACATGAGC CGGTGAACTC TGAGCCTCCT 
    GTACCGCCTC CCAAACCTGA ATCTGTCCTG TCTCCAGGAG AACCGCCCCA TTCTGCAGCT 
    TCCAAACCAT TGCCGCCTCC TCCCAGAGAT GATGACGATG ATGATGACGA TGATGATGAC 
    CCTGCCATGA AGCCCCAGTC TGTGCTCAAC AGGGTCAAAA TGTTCGAAAA CAAACGCTCT 
    GTGTCGGTAG ACCGAGCCAA GGACGCACCT GACGTCACTG GAATTAGGCC CACAGATCTC 
    CCCAAACCTG CGAGCACCCC TGGACCTGTG CTCAAAGCCA ACTCTCTCAG CAACCTGGAG 
    CAGGACAAAC CCTCCTACAG AGCTCCAGAG CCGCAGAAAC CTCAGACGCG TGTTGGGGAC 
    GATGTGGTTC GCTCCAACCA CTACGACCCT GATGATGATG AAGAGTACTA CAGAAAACAG 
    CTCTCATTTT TCGACCGCCG CAGTTTCGAC AGTAAGGTCA TGAATCCACC CAGCGCTGGA 
    GTAAACCGCT TCCATGAGCC GCCCAAACCA CCCCAGACAC AGATCGCTTA TCCTTTCGCT 
    AGGACGGAGT CTGTGGAGAA GGTGAGTCCT GTGGACAAGA GATACGAGCC TCTGCCGCCA 
    GTCAACCCTT CACCTGCTCC ATACAGCCAG AACACACCTG TCGCTCCGCC CACATCCCTG 
    CCCAAACTCA CCAACATCGA GGTGAACTCT CTGCCAGATC CTCACAGCTC TCCCAAAGCC 
    AAACCGGACC TGTCTGCTCT GAGAGCTCCG ACCCGGGACG AGCCCATCCA GAGCAGCTAC 
    TTGCCCCCCA AGTCCACCAT CAATGGCATG GATGCTCCTC CCAAAACACT CGGCGTGCCG 
    ACCAGCTACA ACCGCTACGT CCCCAAGCCT TACACCAGCT CGGCGCGACC CTTCGAGCGC 
    AAGTTCGAGA GTCCGAAATT CAACCACAAC CTGCTTCCCA ACGACACGCA GTCCAAACCC 
    AGTGTCAACA ACAACCTGAA GCCTCAGATC TCCCCACAAC CCCAGGACAC GGACAGCGGC 
    GTCGACACCT TCACTCGCAC CATGGACAAC AGGCCAAAAT ATCAGCACAA CAACATCAAC 
    GCCATTCCCA AAGCCGTCCC AGTCAGCCCC AGTGCACTGG ATGATGATGA AGAAGATGAG 
    GGACACACAG TTGTGGCCAC GGCGCGGGGC ATCTTTAACT GTAATGGCGG GGTGTTGAGC 
    TCTATAGAGA CGGGCGTCAG CATCATTATC CCACAGGGAG CCATTCCTGA TAGTGTGGAG 
    CAGGAGATCT ACTTCAAAGT GTGCCGGGAC AACAGCATCC TGCCTCCTCT GGATAAGGAG 
    AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG ATGTGTGGTC CTCACGGCCT GAAGTTCCTG 
    AAGCCTGTGG AGCTGCGCTT GCCACACTGT GCGTCTATGA CCCCTGATGG TTGGTCTTTT 
    GCTCTAAAAT CCTCCGACTC CTCGTCGGGT GACCCCAAAT GCTGGCAAAA CAAGTGTCTA 
    CCAGGAGACC CCAATTACCT GGTGGGCGCA AACTGTGTGT CTGTGCTGAT CGACCACTTC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470737.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326412.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X19, mRNA.
    Price & Availability↑
    CloneID ODa44630
    Clone ID Related Accession (Same CDS sequence) XM_021470737.1
    Accession Version XM_021470737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGATGAGA CGGTAATATG GGAACAGCAC ACTGTAACTC TACACAGGGC TCCAGGGTTT 
    GGCTTCGGGA TCGCCATCTC CGGCGGACGG GACAACCCTC ACTTCCAGAG CGGGGAGACG 
    TCCATCGTCA TATCAGATGT GCTGAAGGGA GGCCCCGCCG AGGGCCTGCT GCAGGAGAAT 
    GACAGAGTGG TGATGGTCAA CGCCGTCTCC ATGGACAATG TGGATCACGC TTACGCAGTG 
    CAGCAGCTCC GCAAAAGTGG CAAGAATGCA AAAATAACCA TCAGGAGAAA GCGTAAGGTG 
    CAGATCCCGT CAGGCCGGCC GGGTGAACGC GAGACTATGT CAGAGCATGA CGAGGACGAC 
    GACAGCTACG AGGATGAGAT CTACGAGTCC CGGAGCGCGC GAAGCGGACC CAGCGCCTAC 
    AGTGGAGGTG GAGGCGGCGG CGGAGGTGGA GGAGCCTCGG GACGGAGGAG CGGCAGAGGC 
    GACAGGCTGA GTGGGAGGAG GGAGCGGGAC AGAGAGCGCA GCGGCTCCAG AGAGAGGAGT 
    CTGTCCCCGC GCTCGGACCG CCACTCTGTG TCCTCCAACC TCCCCCCACG GCCAGCCAAA 
    GTCACACTCG TCAAATCCCG CAAGAATGAA GCATGTATGG CGTCAGGAAG TTCTCAGAAG 
    GTGACATCAG CAGAATACGG GCTTCGCCTG GCCAGCCACA TCTTCGTGAA GGACATTTCC 
    CCCGAGAGCC TGGCAGCCCG AGACGGAAAC ATCCAAGAGG GAGACGTGGT GCTTAAGATT 
    AATGGCACGG TAACAGAGAA TTTGTCCCTG ATCGATGCTA AGAAGCTGAT CGAGAGGTCA 
    AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
    GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGATAT TTCAGACATC 
    CACTCCGTGG CCTCTGATCA TTCCAACCGA TCCCACGACC GGAAGAGGAG CAGCCGCTCT 
    CGTTCTCCAG ACCGCCGCTC CGAACCCTCA GACCACTCCA GACACTCTCC ACCACAGATC 
    AGCAACGGCA GGGCCACAGG GCCGACGGCT CAGAGAACGC GGCCAGTTCA CAGGAGCCGT 
    GATGAAGACA GGATCTCCAA GGCCGGGCCG CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG 
    ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG 
    CCCAAACCTG TGTACGCTCA GCCGGGCCAG CCGGACGTGG ACCTGCCTGT TAGTCCGGCA 
    GATGCTCCTG TGCCCAGCGT CACACATGAT GACAGCATCT TACGGCCGAG CATGAAGCTG 
    GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC TTGAGGCTCG CTGGGGGGAA CGACGTTGGG 
    ATATTTGTGG CGGGAGTTCT GGAGGACAGT CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA 
    GACCAGATTC TCAGGGTAAA TAATGTGGAT TTTGCAAATA TCATCCGAGA GGAGGCAGTG 
    CTGTTCCTGC TGGATCTGCC CAAAGGAGAG GAGGTCACCA TCCTGGCCCA GAAGAAGAAA 
    GACGTGTACC GCCGCATTGT AGAGTCTGAC GTGGGAGACT CGTTCTACAT CAGGACTCAT 
    TTTGAATATG AGAAGGAGTC GCCCTATGGC CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA 
    GTCGTCGACA CCCTTTACAA TGGCAAACTG GGCTCCTGGC TCGCCATCCG CATCGGCAAA 
    AACCACCAGG AGGTGGAGCG GGGCATCATC CCCAACAAGA ACAGGGCTGA GCAGTTATCA 
    AGTGTCCAGT ACACTCTCCC AAAGACTCCA GGAGGGGATC GCGCCGACTT CTGGAGATTC 
    AGAGGCCTCC GCAGCTCCAA ACGAAACCTG AGGAAGAGCA GAGAAGACCT GTCTGCTCAG 
    CCGGTCCAAA CCAAATTCCC TGCTTATGAA AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG 
    AGGCCAGTGG TCATTTTCGG ACCGATCGCG GATGTGGCTA GAGAGAAGCT GTCCAGAGAG 
    GAGACTGACC TCTTCGAACT TGCAAAGAGT GAACCCAGAG ATGCGGGCAC AGACCAGCGC 
    AGCTCCGGAA TCATCCGTCT CCATACCATT AAACAGATCA TTGACCGAGA CAAGCACGCG 
    GTGCTGGACA TCACCCCTAA TGCGGTGGAC CGTCTGAACT ACGCTCAGTG GTACCCCATC 
    GTGGTGTTTC TGAATCCAGA CAGCAAACAG GGCGTCAAGA ACATGAGGAC CAGGCTCTGT 
    CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC TACGAGCGTG CTCTCAAACT GAGGAAGAAC 
    AACCATCACC TTTTCACCAC CACTATTAAC TTGAACAACA TGAATGACGG GTGGTACGGT 
    GCTTTGAAAG AGAGCATCCA GCAGCAGCAG AACCAGTTGG TCTGGGTCTC AGAGGGCAAG 
    GCCGACGGCG CTCCTGACGA TGACCTTGAC ATCCATGACG ACCGCCTCTC CTACCTCTCG 
    GCTCCGGGCA GCGAGTACAG CATGTACAGC ACCGACAGCC GCCACACGTC CGACTATGAG 
    GACACCGACA CGGAGGGTGG TGCCTACACC GACCAGGAGC TGGACGAGAC CATGAACGAT 
    GAGGTGGGCC TGCCTAGCGA GCCTGCCATC ACACGCTCAT CTGAGCCTGT GCGAGAGGAC 
    CCGCCAGTCA TTCAGGACGC AGCTGGATAC CCCAGCTACC AGCATGCGGT ACCGCAACCT 
    GAAGCGGTGA GCCGCATTGA CCCGGCCGGG TTTAAGATGG CAGCGCCGCA GCAGCAAGCT 
    GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC TCTGCAGCGG TCCAGCCCAC CGCAACACTA 
    GCGGGTACGA ACCCCGAGGA GCCGCCTGGC ACCCCTCAGG CCGACTCCCT TAACAGCCCC 
    GTCCCTGTTC CCGACCCCCA GCCCGAACCT GAGCTCGCTC AGCCCCCAAC ACACGACCCC 
    CACCAGTCGC TTCCTCCTGG CCCAGATCCA AAGATGTATA AAAAGGATCT GTACAGCATG 
    GACGAGCCGG TGCGAGTGAC CCACGGCGTG AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG 
    CCTTTAGGCC CCCCAACCTC GCTCTCCTAC AGCCACCAGC CTGTCTACCA GGACCAACAG 
    CCATACCGCC AGTACGACCA CCCGCCTTAT GGCTATGACG GTGGCGGCTA CGCACAACCA 
    AAGCCTCACA ACTACGACCC GCACCTGCAC TACGACAACC GTGTGCCTCA CTATAACGAG 
    CAGTGGCCCA CGTACGACCA GCAGCAGCAG CAGCAGACTT CCCCCCAGCC GCCACCTGCG 
    TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG CCTCCACCAC CGATGGGATA TGAGCCACGC 
    TCCCCATACG AGGACGGACC TAACAGGGAT TTCAGCCCTC CTCAGTCCCA GTATGACGGT 
    GTGTCCCCAA TGGGCTACGA TCACCGGCCG CGGCACCCCA AACCTGCACC GGTCCGATAT 
    GAAGAGCCTC CACCTCCACC TCCTCCGGTG TCCTACGATG CACGGTCACC TTTCGAACCC 
    GAGCCGCATG CTTTCCCAGG CAACACACAT CGCTCGCCAG ACCCACCGAA ACAGTATTAT 
    GGAGACGCCG CTATGAGGCC CTTGTACAAC CCTGGACCTC CGAACAGAGT GTACAAACAT 
    GAGCCGGTGA ACTCTGAGCC TCCTGTACCG CCTCCCAAAC CTGAATCTGT CCTGTCTCCA 
    GGAGAACCGC CCCATTCTGC AGCTTCCAAA CCATTGCCGC CTCCTCCCAG AGATGATGAC 
    GATGATGATG ACGATGATGA TGACCCTGCC ATGAAGCCCC AGTCTGTGCT CAACAGGGTC 
    AAAATGTTCG AAAACAAACG CTCTGTGTCG GTAGACCGAG CCAAGGACGC ACCTGACGTC 
    ACTGGAATTA GGCCCACAGA TCTCCCCAAA CCTGCGAGCA CCCCTGGACC TGTGCTCAAA 
    GCCAACTCTC TCAGCAACCT GGAGCAGGAC AAACCCTCCT ACAGAGCTCC AGAGCCGCAG 
    AAACCTCAGA CGCGTGTTGG GGACGATGTG GTTCGCTCCA ACCACTACGA CCCTGATGAT 
    GATGAAGAGT ACTACAGAAA ACAGCTCTCA TTTTTCGACC GCCGCAGTTT CGACAGTAAG 
    GTCATGAATC CACCCAGCGC TGGAGTAAAC CGCTTCCATG AGCCGCCCAA ACCACCCCAG 
    ACACAGATCG CTTATCCTTT CGCTAGGACG GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC 
    AAGAGATACG AGCCTCTGCC GCCAGTCAAC CCTTCACCTG CTCCATACAG CCAGAACACA 
    CCTGTCGCTC CGCCCACATC CCTGCCCAAA CTCACCAACA TCGAGGTGAA CTCTCTGCCA 
    GATCCTCACA GCTCTCCCAA AGCCAAACCG GACCTGTCTG CTCTGAGAGC TCCGACCCGG 
    GACGAGCCCA TCCAGAGCAG CTACTTGCCC CCCAAGTCCA CCATCAATGG CATGGATGCT 
    CCTCCCAAAA CACTCGGCGT GCCGACCAGC TACAACCGCT ACGTCCCCAA GCCTTACACC 
    AGCTCGGCGC GACCCTTCGA GCGCAAGTTC GAGAGTCCGA AATTCAACCA CAACCTGCTT 
    CCCAACGACA CGCAGTCCAA ACCCAGTGTC AACAACAACC TGAAGCCTCA GATCTCCCCA 
    CAACCCCAGG ACACGGACAG CGGCGTCGAC ACCTTCACTC GCACCATGGA CAACAGGCCA 
    AAATATCAGC ACAACAACAT CAACGCCATT CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA 
    CTGGATGATG ATGAAGAAGA TGAGGGACAC ACAGTTGTGG CCACGGCGCG GGGCATCTTT 
    AACTGTAATG GCGGGGTGTT GAGCTCTATA GAGACGGGCG TCAGCATCAT TATCCCACAG 
    GGAGCCATTC CTGATAGTGT GGAGCAGGAG ATCTACTTCA AAGTGTGCCG GGACAACAGC 
    ATCCTGCCTC CTCTGGATAA GGAGAAAGGT GAAACTCTGC TGAGTCCGCT GGTGATGTGT 
    GGTCCTCACG GCCTGAAGTT CCTGAAGCCT GTGGAGCTGC GCTTGCCACA CTGTGCGTCT 
    ATGACCCCTG ATGGTTGGTC TTTTGCTCTA AAATCCTCCG ACTCCTCGTC GGGTGACCCC 
    AAATGCTGGC AAAACAAGTG TCTACCAGGA GACCCCAATT ACCTGGTGGG CGCAAACTGT 
    GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326412.1
    CDS183..5549
    Translation

    Target ORF information:

    RefSeq Version XM_021470737.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGATGAGA CGGTAATATG GGAACAGCAC ACTGTAACTC TACACAGGGC TCCAGGGTTT 
    GGCTTCGGGA TCGCCATCTC CGGCGGACGG GACAACCCTC ACTTCCAGAG CGGGGAGACG 
    TCCATCGTCA TATCAGATGT GCTGAAGGGA GGCCCCGCCG AGGGCCTGCT GCAGGAGAAT 
    GACAGAGTGG TGATGGTCAA CGCCGTCTCC ATGGACAATG TGGATCACGC TTACGCAGTG 
    CAGCAGCTCC GCAAAAGTGG CAAGAATGCA AAAATAACCA TCAGGAGAAA GCGTAAGGTG 
    CAGATCCCGT CAGGCCGGCC GGGTGAACGC GAGACTATGT CAGAGCATGA CGAGGACGAC 
    GACAGCTACG AGGATGAGAT CTACGAGTCC CGGAGCGCGC GAAGCGGACC CAGCGCCTAC 
    AGTGGAGGTG GAGGCGGCGG CGGAGGTGGA GGAGCCTCGG GACGGAGGAG CGGCAGAGGC 
    GACAGGCTGA GTGGGAGGAG GGAGCGGGAC AGAGAGCGCA GCGGCTCCAG AGAGAGGAGT 
    CTGTCCCCGC GCTCGGACCG CCACTCTGTG TCCTCCAACC TCCCCCCACG GCCAGCCAAA 
    GTCACACTCG TCAAATCCCG CAAGAATGAA GCATGTATGG CGTCAGGAAG TTCTCAGAAG 
    GTGACATCAG CAGAATACGG GCTTCGCCTG GCCAGCCACA TCTTCGTGAA GGACATTTCC 
    CCCGAGAGCC TGGCAGCCCG AGACGGAAAC ATCCAAGAGG GAGACGTGGT GCTTAAGATT 
    AATGGCACGG TAACAGAGAA TTTGTCCCTG ATCGATGCTA AGAAGCTGAT CGAGAGGTCA 
    AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
    GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGATAT TTCAGACATC 
    CACTCCGTGG CCTCTGATCA TTCCAACCGA TCCCACGACC GGAAGAGGAG CAGCCGCTCT 
    CGTTCTCCAG ACCGCCGCTC CGAACCCTCA GACCACTCCA GACACTCTCC ACCACAGATC 
    AGCAACGGCA GGGCCACAGG GCCGACGGCT CAGAGAACGC GGCCAGTTCA CAGGAGCCGT 
    GATGAAGACA GGATCTCCAA GGCCGGGCCG CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG 
    ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG 
    CCCAAACCTG TGTACGCTCA GCCGGGCCAG CCGGACGTGG ACCTGCCTGT TAGTCCGGCA 
    GATGCTCCTG TGCCCAGCGT CACACATGAT GACAGCATCT TACGGCCGAG CATGAAGCTG 
    GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC TTGAGGCTCG CTGGGGGGAA CGACGTTGGG 
    ATATTTGTGG CGGGAGTTCT GGAGGACAGT CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA 
    GACCAGATTC TCAGGGTAAA TAATGTGGAT TTTGCAAATA TCATCCGAGA GGAGGCAGTG 
    CTGTTCCTGC TGGATCTGCC CAAAGGAGAG GAGGTCACCA TCCTGGCCCA GAAGAAGAAA 
    GACGTGTACC GCCGCATTGT AGAGTCTGAC GTGGGAGACT CGTTCTACAT CAGGACTCAT 
    TTTGAATATG AGAAGGAGTC GCCCTATGGC CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA 
    GTCGTCGACA CCCTTTACAA TGGCAAACTG GGCTCCTGGC TCGCCATCCG CATCGGCAAA 
    AACCACCAGG AGGTGGAGCG GGGCATCATC CCCAACAAGA ACAGGGCTGA GCAGTTATCA 
    AGTGTCCAGT ACACTCTCCC AAAGACTCCA GGAGGGGATC GCGCCGACTT CTGGAGATTC 
    AGAGGCCTCC GCAGCTCCAA ACGAAACCTG AGGAAGAGCA GAGAAGACCT GTCTGCTCAG 
    CCGGTCCAAA CCAAATTCCC TGCTTATGAA AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG 
    AGGCCAGTGG TCATTTTCGG ACCGATCGCG GATGTGGCTA GAGAGAAGCT GTCCAGAGAG 
    GAGACTGACC TCTTCGAACT TGCAAAGAGT GAACCCAGAG ATGCGGGCAC AGACCAGCGC 
    AGCTCCGGAA TCATCCGTCT CCATACCATT AAACAGATCA TTGACCGAGA CAAGCACGCG 
    GTGCTGGACA TCACCCCTAA TGCGGTGGAC CGTCTGAACT ACGCTCAGTG GTACCCCATC 
    GTGGTGTTTC TGAATCCAGA CAGCAAACAG GGCGTCAAGA ACATGAGGAC CAGGCTCTGT 
    CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC TACGAGCGTG CTCTCAAACT GAGGAAGAAC 
    AACCATCACC TTTTCACCAC CACTATTAAC TTGAACAACA TGAATGACGG GTGGTACGGT 
    GCTTTGAAAG AGAGCATCCA GCAGCAGCAG AACCAGTTGG TCTGGGTCTC AGAGGGCAAG 
    GCCGACGGCG CTCCTGACGA TGACCTTGAC ATCCATGACG ACCGCCTCTC CTACCTCTCG 
    GCTCCGGGCA GCGAGTACAG CATGTACAGC ACCGACAGCC GCCACACGTC CGACTATGAG 
    GACACCGACA CGGAGGGTGG TGCCTACACC GACCAGGAGC TGGACGAGAC CATGAACGAT 
    GAGGTGGGCC TGCCTAGCGA GCCTGCCATC ACACGCTCAT CTGAGCCTGT GCGAGAGGAC 
    CCGCCAGTCA TTCAGGACGC AGCTGGATAC CCCAGCTACC AGCATGCGGT ACCGCAACCT 
    GAAGCGGTGA GCCGCATTGA CCCGGCCGGG TTTAAGATGG CAGCGCCGCA GCAGCAAGCT 
    GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC TCTGCAGCGG TCCAGCCCAC CGCAACACTA 
    GCGGGTACGA ACCCCGAGGA GCCGCCTGGC ACCCCTCAGG CCGACTCCCT TAACAGCCCC 
    GTCCCTGTTC CCGACCCCCA GCCCGAACCT GAGCTCGCTC AGCCCCCAAC ACACGACCCC 
    CACCAGTCGC TTCCTCCTGG CCCAGATCCA AAGATGTATA AAAAGGATCT GTACAGCATG 
    GACGAGCCGG TGCGAGTGAC CCACGGCGTG AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG 
    CCTTTAGGCC CCCCAACCTC GCTCTCCTAC AGCCACCAGC CTGTCTACCA GGACCAACAG 
    CCATACCGCC AGTACGACCA CCCGCCTTAT GGCTATGACG GTGGCGGCTA CGCACAACCA 
    AAGCCTCACA ACTACGACCC GCACCTGCAC TACGACAACC GTGTGCCTCA CTATAACGAG 
    CAGTGGCCCA CGTACGACCA GCAGCAGCAG CAGCAGACTT CCCCCCAGCC GCCACCTGCG 
    TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG CCTCCACCAC CGATGGGATA TGAGCCACGC 
    TCCCCATACG AGGACGGACC TAACAGGGAT TTCAGCCCTC CTCAGTCCCA GTATGACGGT 
    GTGTCCCCAA TGGGCTACGA TCACCGGCCG CGGCACCCCA AACCTGCACC GGTCCGATAT 
    GAAGAGCCTC CACCTCCACC TCCTCCGGTG TCCTACGATG CACGGTCACC TTTCGAACCC 
    GAGCCGCATG CTTTCCCAGG CAACACACAT CGCTCGCCAG ACCCACCGAA ACAGTATTAT 
    GGAGACGCCG CTATGAGGCC CTTGTACAAC CCTGGACCTC CGAACAGAGT GTACAAACAT 
    GAGCCGGTGA ACTCTGAGCC TCCTGTACCG CCTCCCAAAC CTGAATCTGT CCTGTCTCCA 
    GGAGAACCGC CCCATTCTGC AGCTTCCAAA CCATTGCCGC CTCCTCCCAG AGATGATGAC 
    GATGATGATG ACGATGATGA TGACCCTGCC ATGAAGCCCC AGTCTGTGCT CAACAGGGTC 
    AAAATGTTCG AAAACAAACG CTCTGTGTCG GTAGACCGAG CCAAGGACGC ACCTGACGTC 
    ACTGGAATTA GGCCCACAGA TCTCCCCAAA CCTGCGAGCA CCCCTGGACC TGTGCTCAAA 
    GCCAACTCTC TCAGCAACCT GGAGCAGGAC AAACCCTCCT ACAGAGCTCC AGAGCCGCAG 
    AAACCTCAGA CGCGTGTTGG GGACGATGTG GTTCGCTCCA ACCACTACGA CCCTGATGAT 
    GATGAAGAGT ACTACAGAAA ACAGCTCTCA TTTTTCGACC GCCGCAGTTT CGACAGTAAG 
    GTCATGAATC CACCCAGCGC TGGAGTAAAC CGCTTCCATG AGCCGCCCAA ACCACCCCAG 
    ACACAGATCG CTTATCCTTT CGCTAGGACG GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC 
    AAGAGATACG AGCCTCTGCC GCCAGTCAAC CCTTCACCTG CTCCATACAG CCAGAACACA 
    CCTGTCGCTC CGCCCACATC CCTGCCCAAA CTCACCAACA TCGAGGTGAA CTCTCTGCCA 
    GATCCTCACA GCTCTCCCAA AGCCAAACCG GACCTGTCTG CTCTGAGAGC TCCGACCCGG 
    GACGAGCCCA TCCAGAGCAG CTACTTGCCC CCCAAGTCCA CCATCAATGG CATGGATGCT 
    CCTCCCAAAA CACTCGGCGT GCCGACCAGC TACAACCGCT ACGTCCCCAA GCCTTACACC 
    AGCTCGGCGC GACCCTTCGA GCGCAAGTTC GAGAGTCCGA AATTCAACCA CAACCTGCTT 
    CCCAACGACA CGCAGTCCAA ACCCAGTGTC AACAACAACC TGAAGCCTCA GATCTCCCCA 
    CAACCCCAGG ACACGGACAG CGGCGTCGAC ACCTTCACTC GCACCATGGA CAACAGGCCA 
    AAATATCAGC ACAACAACAT CAACGCCATT CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA 
    CTGGATGATG ATGAAGAAGA TGAGGGACAC ACAGTTGTGG CCACGGCGCG GGGCATCTTT 
    AACTGTAATG GCGGGGTGTT GAGCTCTATA GAGACGGGCG TCAGCATCAT TATCCCACAG 
    GGAGCCATTC CTGATAGTGT GGAGCAGGAG ATCTACTTCA AAGTGTGCCG GGACAACAGC 
    ATCCTGCCTC CTCTGGATAA GGAGAAAGGT GAAACTCTGC TGAGTCCGCT GGTGATGTGT 
    GGTCCTCACG GCCTGAAGTT CCTGAAGCCT GTGGAGCTGC GCTTGCCACA CTGTGCGTCT 
    ATGACCCCTG ATGGTTGGTC TTTTGCTCTA AAATCCTCCG ACTCCTCGTC GGGTGACCCC 
    AAATGCTGGC AAAACAAGTG TCTACCAGGA GACCCCAATT ACCTGGTGGG CGCAAACTGT 
    GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_009298141.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296416.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA.
    Price & Availability↑
    CloneID ODa44631
    Clone ID Related Accession (Same CDS sequence) XM_009298141.2 , XM_009298141.3
    Accession Version XM_009298141.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X15
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
    CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
    GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
    GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
    AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
    AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
    GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
    GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
    GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
    GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
    AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
    TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
    AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
    GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
    AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
    GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
    GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
    CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
    CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
    CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
    GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
    GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
    AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
    CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
    CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
    GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
    CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
    CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
    AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
    AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
    GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
    CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
    CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
    ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
    CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
    CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
    TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
    GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
    CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
    CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
    AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
    CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
    TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
    TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
    GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
    CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
    TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
    AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
    GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
    GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
    CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
    CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
    TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
    TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
    TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
    TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
    ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
    CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
    AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
    AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
    AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
    AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
    TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
    AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
    GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
    ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
    TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
    ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
    GAGCTGCGCT TGCCACACTG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296416.1
    CDS115..6108
    Translation

    Target ORF information:

    RefSeq Version XM_009298141.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298141.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
    CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
    GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
    GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
    AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
    AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
    GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
    GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
    GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
    GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
    AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
    TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
    AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
    GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
    AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
    GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
    GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
    CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
    CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
    CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
    GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
    GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
    AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
    CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
    CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
    GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
    CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
    CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
    AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
    AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
    GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
    CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
    CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
    ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
    CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
    CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
    TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
    GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
    CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
    CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
    AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
    CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
    TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
    TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
    GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
    CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
    TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
    AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
    GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
    GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
    CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
    CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
    TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
    TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
    TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
    TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
    ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
    CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
    AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
    AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
    AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
    AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
    TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
    AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
    GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
    ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
    TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
    ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
    GAGCTGCGCT TGCCACACTG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_009298141.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296416.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X20, mRNA.
    Price & Availability↑
    CloneID ODa44631
    Clone ID Related Accession (Same CDS sequence) XM_009298141.2 , XM_009298141.3
    Accession Version XM_009298141.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
    CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
    GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
    GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
    AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
    AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
    GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
    GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
    GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
    GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
    AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
    TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
    AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
    GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
    AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
    GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
    GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
    CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
    CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
    CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
    GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
    GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
    AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
    CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
    CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
    GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
    CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
    CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
    AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
    AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
    GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
    CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
    CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
    ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
    CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
    CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
    TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
    GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
    CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
    CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
    AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
    CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
    TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
    TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
    GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
    CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
    TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
    AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
    GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
    GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
    CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
    CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
    TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
    TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
    TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
    TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
    ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
    CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
    AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
    AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
    AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
    AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
    TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
    AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
    GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
    ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
    TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
    ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
    GAGCTGCGCT TGCCACACTG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296416.1
    CDS110..6103
    Translation

    Target ORF information:

    RefSeq Version XM_009298141.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298141.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
    CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
    GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
    GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
    AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
    AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
    GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
    GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
    GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
    GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
    AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
    TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
    AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
    GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
    AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
    GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
    GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
    CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
    CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
    CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
    GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
    GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
    AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
    CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
    CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
    GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
    CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
    CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
    AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
    AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
    GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
    CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
    CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
    ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
    CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
    CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
    TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
    GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
    CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
    CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
    AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
    CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
    TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
    TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
    GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
    CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
    TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
    AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
    GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
    GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
    CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
    CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
    TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
    TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
    TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
    TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
    ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
    CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
    AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
    AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
    AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
    AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
    TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
    AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
    GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
    ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
    TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
    ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
    GAGCTGCGCT TGCCACACTG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354175.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209664.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID ODa57885
    Clone ID Related Accession (Same CDS sequence) XM_017354175.1
    Accession Version XM_017354175.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
    CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
    CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
    TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
    GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
    CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
    CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
    AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
    CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
    TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
    TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
    GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
    CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
    TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
    AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
    GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
    GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
    CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
    CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
    TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
    TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
    TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
    TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
    ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
    CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
    AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
    AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
    AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
    AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
    TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
    AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
    GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
    ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
    TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
    ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
    GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
    TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209664.1
    CDS114..5987
    Translation

    Target ORF information:

    RefSeq Version XM_017354175.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
    CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
    CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
    TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
    GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
    CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
    CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
    AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
    CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
    TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
    TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
    GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
    CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
    TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
    AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
    GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
    GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
    CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
    CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
    TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
    TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
    TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
    TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
    ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
    CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
    AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
    AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
    AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
    AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
    TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
    AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
    GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
    ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
    TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
    ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
    GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
    TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354176.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209665.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA.
    Price & Availability↑
    CloneID ODa57886
    Clone ID Related Accession (Same CDS sequence) XM_017354176.1
    Accession Version XM_017354176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGAATT ACCAGAAATA CATTACTGTT ATGCAGCTGG CTCTAGGGGT GACTGCCTAC 
    AACAGAGACC GTAGCCTGCC TCCCAGAAAA CATATGTGGG TCTTCCCCAA ACACGACAAA 
    GACAAATACG GCCCTGCAGT TTATTATCCG AAGTTCAACG CCTACATCCC CAACCCAGAC 
    AAAGTCTCTG TGCTGGATGA ATCTGAGGGA TCGGGCCACG GCAAACCATC CCTGCGCAGA 
    ATCAAGGGCC GAATCCACCG CAGCAAGAGC CTGGACAGCA TCGACCTGCT GGACTCCAAC 
    AGTGCAGCAA TGGATGAGAC GGTAATATGG GAACAGCACA CTGTAACTCT ACACAGGGCT 
    CCAGGGTTTG GCTTCGGGAT CGCCATCTCC GGCGGACGGG ACAACCCTCA CTTCCAGAGC 
    GGGGAGACGT CCATCGTCAT ATCAGATGTG CTGAAGGGAG GCCCCGCCGA GGGCCTGCTG 
    CAGGAGAATG ACAGAGTGGT GATGGTCAAC GCCGTCTCCA TGGACAATGT GGATCACGCT 
    TACGCAGTGC AGCAGCTCCG CAAAAGTGGC AAGAATGCAA AAATAACCAT CAGGAGAAAG 
    CGTAAGGTGC AGATCCCGTC AGGCCGGCCG GGTGAACGCG AGACTATGTC AGAGCATGAC 
    GAGGACGACG ACAGCTACGA GGATGAGATC TACGAGTCCC GGAGCGCGCG AAGCGGACCC 
    AGCGCCTACA GTGGAGGTGG AGGCGGCGGC GGAGGTGGAG GAGCCTCGGG ACGGAGGAGC 
    GGCAGAGGCG ACAGGCTGAG TGGGAGGAGG GAGCGGGACA GAGAGCGCAG CGGCTCCAGA 
    GAGAGGAGTC TGTCCCCGCG CTCGGACCGC CACTCTGTGT CCTCCAACCT CCCCCCACGG 
    CCAGCCAAAG TCACACTCGT CAAATCCCGC AAGAATGAAG CAGAATACGG GCTTCGCCTG 
    GCCAGCCACA TCTTCGTGAA GGACATTTCC CCCGAGAGCC TGGCAGCCCG AGACGGAAAC 
    ATCCAAGAGG GAGACGTGGT GCTTAAGATT AATGGCACGG TAACAGAGAA TTTGTCCCTG 
    ATCGATGCTA AGAAGCTGAT CGAGAGGTCA AAGGGAAAGC TGAAGATGGT GGTGCAGAGA 
    GACGAGCGGG CGACGCTGCT GAACATCCCC GATCTGGACG ACAGCATACC TTCTGCTAAT 
    GCCTCGGACA GAGATGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
    TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
    GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
    CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
    CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
    ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
    CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
    GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
    TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
    CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
    TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
    GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
    GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
    CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
    GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
    CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
    GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
    AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
    AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
    GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
    GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
    AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
    CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
    GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
    TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
    TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
    AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
    ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
    ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
    GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
    ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
    CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
    TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
    TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
    ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
    GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
    AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
    AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
    AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
    GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
    TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
    CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
    CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
    TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
    CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
    TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
    CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
    CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
    CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
    CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
    ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
    GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
    CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
    AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
    GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
    TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
    CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
    GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
    CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
    CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209665.1
    CDS431..6067
    Translation

    Target ORF information:

    RefSeq Version XM_017354176.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGTGAATT ACCAGAAATA CATTACTGTT ATGCAGCTGG CTCTAGGGGT GACTGCCTAC 
    AACAGAGACC GTAGCCTGCC TCCCAGAAAA CATATGTGGG TCTTCCCCAA ACACGACAAA 
    GACAAATACG GCCCTGCAGT TTATTATCCG AAGTTCAACG CCTACATCCC CAACCCAGAC 
    AAAGTCTCTG TGCTGGATGA ATCTGAGGGA TCGGGCCACG GCAAACCATC CCTGCGCAGA 
    ATCAAGGGCC GAATCCACCG CAGCAAGAGC CTGGACAGCA TCGACCTGCT GGACTCCAAC 
    AGTGCAGCAA TGGATGAGAC GGTAATATGG GAACAGCACA CTGTAACTCT ACACAGGGCT 
    CCAGGGTTTG GCTTCGGGAT CGCCATCTCC GGCGGACGGG ACAACCCTCA CTTCCAGAGC 
    GGGGAGACGT CCATCGTCAT ATCAGATGTG CTGAAGGGAG GCCCCGCCGA GGGCCTGCTG 
    CAGGAGAATG ACAGAGTGGT GATGGTCAAC GCCGTCTCCA TGGACAATGT GGATCACGCT 
    TACGCAGTGC AGCAGCTCCG CAAAAGTGGC AAGAATGCAA AAATAACCAT CAGGAGAAAG 
    CGTAAGGTGC AGATCCCGTC AGGCCGGCCG GGTGAACGCG AGACTATGTC AGAGCATGAC 
    GAGGACGACG ACAGCTACGA GGATGAGATC TACGAGTCCC GGAGCGCGCG AAGCGGACCC 
    AGCGCCTACA GTGGAGGTGG AGGCGGCGGC GGAGGTGGAG GAGCCTCGGG ACGGAGGAGC 
    GGCAGAGGCG ACAGGCTGAG TGGGAGGAGG GAGCGGGACA GAGAGCGCAG CGGCTCCAGA 
    GAGAGGAGTC TGTCCCCGCG CTCGGACCGC CACTCTGTGT CCTCCAACCT CCCCCCACGG 
    CCAGCCAAAG TCACACTCGT CAAATCCCGC AAGAATGAAG CAGAATACGG GCTTCGCCTG 
    GCCAGCCACA TCTTCGTGAA GGACATTTCC CCCGAGAGCC TGGCAGCCCG AGACGGAAAC 
    ATCCAAGAGG GAGACGTGGT GCTTAAGATT AATGGCACGG TAACAGAGAA TTTGTCCCTG 
    ATCGATGCTA AGAAGCTGAT CGAGAGGTCA AAGGGAAAGC TGAAGATGGT GGTGCAGAGA 
    GACGAGCGGG CGACGCTGCT GAACATCCCC GATCTGGACG ACAGCATACC TTCTGCTAAT 
    GCCTCGGACA GAGATGATAT TTCAGACATC CACTCCGTGG CCTCTGATCA TTCCAACCGA 
    TCCCACGACC GGAAGAGGAG CAGCCGCTCT CGTTCTCCAG ACCGCCGCTC CGAACCCTCA 
    GACCACTCCA GACACTCTCC ACCACAGATC AGCAACGGCA GGGCCACAGG GCCGACGGCT 
    CAGAGAACGC GGCCAGTTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
    CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
    ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
    CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
    GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
    TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
    CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
    TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
    GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
    GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
    CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
    GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
    CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
    GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
    AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
    AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
    GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
    GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
    AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
    CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
    GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
    TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
    TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
    AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
    ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
    ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
    GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
    ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
    CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
    TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
    TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
    ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
    GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
    AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
    AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
    AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
    GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
    TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
    CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
    CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
    TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
    CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
    TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
    CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
    CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
    CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
    CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
    ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
    GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
    CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
    AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
    GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
    TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
    CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
    GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
    CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
    CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354177.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209666.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA.
    Price & Availability↑
    CloneID ODa57887
    Clone ID Related Accession (Same CDS sequence) XM_017354177.1
    Accession Version XM_017354177.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGCCC GGGCTGCCTC TAATAAGAGT GCAGCAATGG ATGAGACGGT AATATGGGAA 
    CAGCACACTG TAACTCTACA CAGGGCTCCA GGGTTTGGCT TCGGGATCGC CATCTCCGGC 
    GGACGGGACA ACCCTCACTT CCAGAGCGGG GAGACGTCCA TCGTCATATC AGATGTGCTG 
    AAGGGAGGCC CCGCCGAGGG CCTGCTGCAG GAGAATGACA GAGTGGTGAT GGTCAACGCC 
    GTCTCCATGG ACAATGTGGA TCACGCTTAC GCAGTGCAGC AGCTCCGCAA AAGTGGCAAG 
    AATGCAAAAA TAACCATCAG GAGAAAGCGT AAGGTGCAGA TCCCGTCAGG CCGGCCGGGT 
    GAACGCGAGA CTATGTCAGA GCATGACGAG GACGACGACA GCTACGAGGA TGAGATCTAC 
    GAGTCCCGGA GCGCGCGAAG CGGACCCAGC GCCTACAGTG GAGGTGGAGG CGGCGGCGGA 
    GGTGGAGGAG CCTCGGGACG GAGGAGCGGC AGAGGCGACA GGCTGAGTGG GAGGAGGGAG 
    CGGGACAGAG AGCGCAGCGG CTCCAGAGAG AGGAGTCTGT CCCCGCGCTC GGACCGCCAC 
    TCTGTGTCCT CCAACCTCCC CCCACGGCCA GCCAAAGTCA CACTCGTCAA ATCCCGCAAG 
    AATGAAGCAG AATACGGGCT TCGCCTGGCC AGCCACATCT TCGTGAAGGA CATTTCCCCC 
    GAGAGCCTGG CAGCCCGAGA CGGAAACATC CAAGAGGGAG ACGTGGTGCT TAAGATTAAT 
    GGCACGGTAA CAGAGAATTT GTCCCTGATC GATGCTAAGA AGCTGATCGA GAGGTCAAAG 
    GGAAAGCTGA AGATGGTGGT GCAGAGAGAC GAGCGGGCGA CGCTGCTGAA CATCCCCGAT 
    CTGGACGACA GCATACCTTC TGCTAATGCC TCGGACAGAG ATGATATTTC AGACATCCAC 
    TCCGTGGCCT CTGATCATTC CAACCGATCC CACGACCGGA AGAGGAGCAG CCGCTCTCGT 
    TCTCCAGACC GCCGCTCCGA ACCCTCAGAC CACTCCAGAC ACTCTCCACC ACAGATCAGC 
    AACGGCAGGG CCACAGGGCC GACGGCTCAG AGAACGCGGC CAGTTCACAG GAGCCGTGAT 
    GAAGACAGGA TCTCCAAGGC CGGGCCGCTT CCTCTACCTG CTAAGATGGT GGAGGAGATG 
    CCGAAAGAGC AGAGCGCAGC CAGAGAAACA GAGAAACAGC TCCCCCCTCT GCCAGAGCCC 
    AAACCTGTGT ACGCTCAGCC GGGCCAGCCG GACGTGGACC TGCCTGTTAG TCCGGCAGAT 
    GCTCCTGTGC CCAGCGTCAC ACATGATGAC AGCATCTTAC GGCCGAGCAT GAAGCTGGTG 
    AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG AGGCTCGCTG GGGGGAACGA CGTTGGGATA 
    TTTGTGGCGG GAGTTCTGGA GGACAGTCCT GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC 
    CAGATTCTCA GGGTAAATAA TGTGGATTTT GCAAATATCA TCCGAGAGGA GGCAGTGCTG 
    TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG GTCACCATCC TGGCCCAGAA GAAGAAAGAC 
    GTGTACCGCC GCATTGTAGA GTCTGACGTG GGAGACTCGT TCTACATCAG GACTCATTTT 
    GAATATGAGA AGGAGTCGCC CTATGGCCTG AGCTTCAATA AGGGGGAGGT CTTCAGAGTC 
    GTCGACACCC TTTACAATGG CAAACTGGGC TCCTGGCTCG CCATCCGCAT CGGCAAAAAC 
    CACCAGGAGG TGGAGCGGGG CATCATCCCC AACAAGAACA GGGCTGAGCA GTTATCAAGT 
    GTCCAGTACA CTCTCCCAAA GACTCCAGGA GGGGATCGCG CCGACTTCTG GAGATTCAGA 
    GGCCTCCGCA GCTCCAAACG AAACCTGAGG AAGAGCAGAG AAGACCTGTC TGCTCAGCCG 
    GTCCAAACCA AATTCCCTGC TTATGAAAGA GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG 
    CCAGTGGTCA TTTTCGGACC GATCGCGGAT GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG 
    ACTGACCTCT TCGAACTTGC AAAGAGTGAA CCCAGAGATG CGGGCACAGA CCAGCGCAGC 
    TCCGGAATCA TCCGTCTCCA TACCATTAAA CAGATCATTG ACCGAGACAA GCACGCGGTG 
    CTGGACATCA CCCCTAATGC GGTGGACCGT CTGAACTACG CTCAGTGGTA CCCCATCGTG 
    GTGTTTCTGA ATCCAGACAG CAAACAGGGC GTCAAGAACA TGAGGACCAG GCTCTGTCCC 
    GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC GAGCGTGCTC TCAAACTGAG GAAGAACAAC 
    CATCACCTTT TCACCACCAC TATTAACTTG AACAACATGA ATGACGGGTG GTACGGTGCT 
    TTGAAAGAGA GCATCCAGCA GCAGCAGAAC CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC 
    GACGGCGCTC CTGACGATGA CCTTGACATC CATGACGACC GCCTCTCCTA CCTCTCGGCT 
    CCGGGCAGCG AGTACAGCAT GTACAGCACC GACAGCCGCC ACACGTCCGA CTATGAGGAC 
    ACCGACACGG AGGGTGGTGC CTACACCGAC CAGGAGCTGG ACGAGACCAT GAACGATGAG 
    GTGGGCCTGC CTAGCGAGCC TGCCATCACA CGCTCATCTG AGCCTGTGCG AGAGGACCCG 
    CCAGTCATTC AGGACGCAGC TGGATACCCC AGCTACCAGC ATGCGGTACC GCAACCTGAA 
    GCGGTGAGCC GCATTGACCC GGCCGGGTTT AAGATGGCAG CGCCGCAGCA GCAAGCTGAG 
    GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT GCAGCGGTCC AGCCCACCGC AACACTAGCG 
    GGTACGAACC CCGAGGAGCC GCCTGGCACC CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC 
    CCTGTTCCCG ACCCCCAGCC CGAACCTGAG CTCGCTCAGC CCCCAACACA CGACCCCCAC 
    CAGTCGCTTC CTCCTGGCCC AGATCCAAAG ATGTATAAAA AGGATCTGTA CAGCATGGAC 
    GAGCCGGTGC GAGTGACCCA CGGCGTGAAG CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT 
    TTAGGCCCCC CAACCTCGCT CTCCTACAGC CACCAGCCTG TCTACCAGGA CCAACAGCCA 
    TACCGCCAGT ACGACCACCC GCCTTATGGC TATGACGGTG GCGGCTACGC ACAACCAAAG 
    CCTCACAACT ACGACCCGCA CCTGCACTAC GACAACCGTG TGCCTCACTA TAACGAGCAG 
    TGGCCCACGT ACGACCAGCA GCAGCAGCAG CAGACTTCCC CCCAGCCGCC ACCTGCGTCC 
    GGCTTCCCTC AGGGCCACCA GCCTCCGCCT CCACCACCGA TGGGATATGA GCCACGCTCC 
    CCATACGAGG ACGGACCTAA CAGGGATTTC AGCCCTCCTC AGTCCCAGTA TGACGGTGTG 
    TCCCCAATGG GCTACGATCA CCGGCCGCGG CACCCCAAAC CTGCACCGGT CCGATATGAA 
    GAGCCTCCAC CTCCACCTCC TCCGGTGTCC TACGATGCAC GGTCACCTTT CGAACCCGAG 
    CCGCATGCTT TCCCAGGCAA CACACATCGC TCGCCAGACC CACCGAAACA GTATTATGGA 
    GACGCCGCTA TGAGGCCCTT GTACAACCCT GGACCTCCGA ACAGAGTGTA CAAACATGAG 
    CCGGTGAACT CTGAGCCTCC TGTACCGCCT CCCAAACCTG AATCTGTCCT GTCTCCAGGA 
    GAACCGCCCC ATTCTGCAGC TTCCAAACCA TTGCCGCCTC CTCCCAGAGA TGATGACGAT 
    GATGATGACG ATGATGATGA CCCTGCCATG AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA 
    ATGTTCGAAA ACAAACGCTC TGTGTCGGTA GACCGAGCCA AGGACGCACC TGACGTCACT 
    GGAATTAGGC CCACAGATCT CCCCAAACCT GCGAGCACCC CTGGACCTGT GCTCAAAGCC 
    AACTCTCTCA GCAACCTGGA GCAGGACAAA CCCTCCTACA GAGCTCCAGA GCCGCAGAAA 
    CCTCAGACGC GTGTTGGGGA CGATGTGGTT CGCTCCAACC ACTACGACCC TGATGATGAT 
    GAAGAGTACT ACAGAAAACA GCTCTCATTT TTCGACCGCC GCAGTTTCGA CAGTAAGGTC 
    ATGAATCCAC CCAGCGCTGG AGTAAACCGC TTCCATGAGC CGCCCAAACC ACCCCAGACA 
    CAGATCGCTT ATCCTTTCGC TAGGACGGAG TCTGTGGAGA AGGTGAGTCC TGTGGACAAG 
    AGATACGAGC CTCTGCCGCC AGTCAACCCT TCACCTGCTC CATACAGCCA GAACACACCT 
    GTCGCTCCGC CCACATCCCT GCCCAAACTC ACCAACATCG AGGTGAACTC TCTGCCAGAT 
    CCTCACAGCT CTCCCAAAGC CAAACCGGAC CTGTCTGCTC TGAGAGCTCC GACCCGGGAC 
    GAGCCCATCC AGAGCAGCTA CTTGCCCCCC AAGTCCACCA TCAATGGCAT GGATGCTCCT 
    CCCAAAACAC TCGGCGTGCC GACCAGCTAC AACCGCTACG TCCCCAAGCC TTACACCAGC 
    TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG AGTCCGAAAT TCAACCACAA CCTGCTTCCC 
    AACGACACGC AGTCCAAACC CAGTGTCAAC AACAACCTGA AGCCTCAGAT CTCCCCACAA 
    CCCCAGGACA CGGACAGCGG CGTCGACACC TTCACTCGCA CCATGGACAA CAGGCCAAAA 
    TATCAGCACA ACAACATCAA CGCCATTCCC AAAGCCGTCC CAGTCAGCCC CAGTGCACTG 
    GATGATGATG AAGAAGATGA GGGACACACA GTTGTGGCCA CGGCGCGGGG CATCTTTAAC 
    TGTAATGGCG GGGTGTTGAG CTCTATAGAG ACGGGCGTCA GCATCATTAT CCCACAGGGA 
    GCCATTCCTG ATAGTGTGGA GCAGGAGATC TACTTCAAAG TGTGCCGGGA CAACAGCATC 
    CTGCCTCCTC TGGATAAGGA GAAAGGTGAA ACTCTGCTGA GTCCGCTGGT GATGTGTGGT 
    CCTCACGGCC TGAAGTTCCT GAAGCCTGTG GAGCTGCGCT TGCCACACTG TGCGTCTATG 
    ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGACCCCAAA 
    TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC CCCAATTACC TGGTGGGCGC AAACTGTGTG 
    TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209666.1
    CDS524..5887
    Translation

    Target ORF information:

    RefSeq Version XM_017354177.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCTGCCC GGGCTGCCTC TAATAAGAGT GCAGCAATGG ATGAGACGGT AATATGGGAA 
    CAGCACACTG TAACTCTACA CAGGGCTCCA GGGTTTGGCT TCGGGATCGC CATCTCCGGC 
    GGACGGGACA ACCCTCACTT CCAGAGCGGG GAGACGTCCA TCGTCATATC AGATGTGCTG 
    AAGGGAGGCC CCGCCGAGGG CCTGCTGCAG GAGAATGACA GAGTGGTGAT GGTCAACGCC 
    GTCTCCATGG ACAATGTGGA TCACGCTTAC GCAGTGCAGC AGCTCCGCAA AAGTGGCAAG 
    AATGCAAAAA TAACCATCAG GAGAAAGCGT AAGGTGCAGA TCCCGTCAGG CCGGCCGGGT 
    GAACGCGAGA CTATGTCAGA GCATGACGAG GACGACGACA GCTACGAGGA TGAGATCTAC 
    GAGTCCCGGA GCGCGCGAAG CGGACCCAGC GCCTACAGTG GAGGTGGAGG CGGCGGCGGA 
    GGTGGAGGAG CCTCGGGACG GAGGAGCGGC AGAGGCGACA GGCTGAGTGG GAGGAGGGAG 
    CGGGACAGAG AGCGCAGCGG CTCCAGAGAG AGGAGTCTGT CCCCGCGCTC GGACCGCCAC 
    TCTGTGTCCT CCAACCTCCC CCCACGGCCA GCCAAAGTCA CACTCGTCAA ATCCCGCAAG 
    AATGAAGCAG AATACGGGCT TCGCCTGGCC AGCCACATCT TCGTGAAGGA CATTTCCCCC 
    GAGAGCCTGG CAGCCCGAGA CGGAAACATC CAAGAGGGAG ACGTGGTGCT TAAGATTAAT 
    GGCACGGTAA CAGAGAATTT GTCCCTGATC GATGCTAAGA AGCTGATCGA GAGGTCAAAG 
    GGAAAGCTGA AGATGGTGGT GCAGAGAGAC GAGCGGGCGA CGCTGCTGAA CATCCCCGAT 
    CTGGACGACA GCATACCTTC TGCTAATGCC TCGGACAGAG ATGATATTTC AGACATCCAC 
    TCCGTGGCCT CTGATCATTC CAACCGATCC CACGACCGGA AGAGGAGCAG CCGCTCTCGT 
    TCTCCAGACC GCCGCTCCGA ACCCTCAGAC CACTCCAGAC ACTCTCCACC ACAGATCAGC 
    AACGGCAGGG CCACAGGGCC GACGGCTCAG AGAACGCGGC CAGTTCACAG GAGCCGTGAT 
    GAAGACAGGA TCTCCAAGGC CGGGCCGCTT CCTCTACCTG CTAAGATGGT GGAGGAGATG 
    CCGAAAGAGC AGAGCGCAGC CAGAGAAACA GAGAAACAGC TCCCCCCTCT GCCAGAGCCC 
    AAACCTGTGT ACGCTCAGCC GGGCCAGCCG GACGTGGACC TGCCTGTTAG TCCGGCAGAT 
    GCTCCTGTGC CCAGCGTCAC ACATGATGAC AGCATCTTAC GGCCGAGCAT GAAGCTGGTG 
    AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG AGGCTCGCTG GGGGGAACGA CGTTGGGATA 
    TTTGTGGCGG GAGTTCTGGA GGACAGTCCT GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC 
    CAGATTCTCA GGGTAAATAA TGTGGATTTT GCAAATATCA TCCGAGAGGA GGCAGTGCTG 
    TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG GTCACCATCC TGGCCCAGAA GAAGAAAGAC 
    GTGTACCGCC GCATTGTAGA GTCTGACGTG GGAGACTCGT TCTACATCAG GACTCATTTT 
    GAATATGAGA AGGAGTCGCC CTATGGCCTG AGCTTCAATA AGGGGGAGGT CTTCAGAGTC 
    GTCGACACCC TTTACAATGG CAAACTGGGC TCCTGGCTCG CCATCCGCAT CGGCAAAAAC 
    CACCAGGAGG TGGAGCGGGG CATCATCCCC AACAAGAACA GGGCTGAGCA GTTATCAAGT 
    GTCCAGTACA CTCTCCCAAA GACTCCAGGA GGGGATCGCG CCGACTTCTG GAGATTCAGA 
    GGCCTCCGCA GCTCCAAACG AAACCTGAGG AAGAGCAGAG AAGACCTGTC TGCTCAGCCG 
    GTCCAAACCA AATTCCCTGC TTATGAAAGA GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG 
    CCAGTGGTCA TTTTCGGACC GATCGCGGAT GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG 
    ACTGACCTCT TCGAACTTGC AAAGAGTGAA CCCAGAGATG CGGGCACAGA CCAGCGCAGC 
    TCCGGAATCA TCCGTCTCCA TACCATTAAA CAGATCATTG ACCGAGACAA GCACGCGGTG 
    CTGGACATCA CCCCTAATGC GGTGGACCGT CTGAACTACG CTCAGTGGTA CCCCATCGTG 
    GTGTTTCTGA ATCCAGACAG CAAACAGGGC GTCAAGAACA TGAGGACCAG GCTCTGTCCC 
    GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC GAGCGTGCTC TCAAACTGAG GAAGAACAAC 
    CATCACCTTT TCACCACCAC TATTAACTTG AACAACATGA ATGACGGGTG GTACGGTGCT 
    TTGAAAGAGA GCATCCAGCA GCAGCAGAAC CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC 
    GACGGCGCTC CTGACGATGA CCTTGACATC CATGACGACC GCCTCTCCTA CCTCTCGGCT 
    CCGGGCAGCG AGTACAGCAT GTACAGCACC GACAGCCGCC ACACGTCCGA CTATGAGGAC 
    ACCGACACGG AGGGTGGTGC CTACACCGAC CAGGAGCTGG ACGAGACCAT GAACGATGAG 
    GTGGGCCTGC CTAGCGAGCC TGCCATCACA CGCTCATCTG AGCCTGTGCG AGAGGACCCG 
    CCAGTCATTC AGGACGCAGC TGGATACCCC AGCTACCAGC ATGCGGTACC GCAACCTGAA 
    GCGGTGAGCC GCATTGACCC GGCCGGGTTT AAGATGGCAG CGCCGCAGCA GCAAGCTGAG 
    GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT GCAGCGGTCC AGCCCACCGC AACACTAGCG 
    GGTACGAACC CCGAGGAGCC GCCTGGCACC CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC 
    CCTGTTCCCG ACCCCCAGCC CGAACCTGAG CTCGCTCAGC CCCCAACACA CGACCCCCAC 
    CAGTCGCTTC CTCCTGGCCC AGATCCAAAG ATGTATAAAA AGGATCTGTA CAGCATGGAC 
    GAGCCGGTGC GAGTGACCCA CGGCGTGAAG CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT 
    TTAGGCCCCC CAACCTCGCT CTCCTACAGC CACCAGCCTG TCTACCAGGA CCAACAGCCA 
    TACCGCCAGT ACGACCACCC GCCTTATGGC TATGACGGTG GCGGCTACGC ACAACCAAAG 
    CCTCACAACT ACGACCCGCA CCTGCACTAC GACAACCGTG TGCCTCACTA TAACGAGCAG 
    TGGCCCACGT ACGACCAGCA GCAGCAGCAG CAGACTTCCC CCCAGCCGCC ACCTGCGTCC 
    GGCTTCCCTC AGGGCCACCA GCCTCCGCCT CCACCACCGA TGGGATATGA GCCACGCTCC 
    CCATACGAGG ACGGACCTAA CAGGGATTTC AGCCCTCCTC AGTCCCAGTA TGACGGTGTG 
    TCCCCAATGG GCTACGATCA CCGGCCGCGG CACCCCAAAC CTGCACCGGT CCGATATGAA 
    GAGCCTCCAC CTCCACCTCC TCCGGTGTCC TACGATGCAC GGTCACCTTT CGAACCCGAG 
    CCGCATGCTT TCCCAGGCAA CACACATCGC TCGCCAGACC CACCGAAACA GTATTATGGA 
    GACGCCGCTA TGAGGCCCTT GTACAACCCT GGACCTCCGA ACAGAGTGTA CAAACATGAG 
    CCGGTGAACT CTGAGCCTCC TGTACCGCCT CCCAAACCTG AATCTGTCCT GTCTCCAGGA 
    GAACCGCCCC ATTCTGCAGC TTCCAAACCA TTGCCGCCTC CTCCCAGAGA TGATGACGAT 
    GATGATGACG ATGATGATGA CCCTGCCATG AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA 
    ATGTTCGAAA ACAAACGCTC TGTGTCGGTA GACCGAGCCA AGGACGCACC TGACGTCACT 
    GGAATTAGGC CCACAGATCT CCCCAAACCT GCGAGCACCC CTGGACCTGT GCTCAAAGCC 
    AACTCTCTCA GCAACCTGGA GCAGGACAAA CCCTCCTACA GAGCTCCAGA GCCGCAGAAA 
    CCTCAGACGC GTGTTGGGGA CGATGTGGTT CGCTCCAACC ACTACGACCC TGATGATGAT 
    GAAGAGTACT ACAGAAAACA GCTCTCATTT TTCGACCGCC GCAGTTTCGA CAGTAAGGTC 
    ATGAATCCAC CCAGCGCTGG AGTAAACCGC TTCCATGAGC CGCCCAAACC ACCCCAGACA 
    CAGATCGCTT ATCCTTTCGC TAGGACGGAG TCTGTGGAGA AGGTGAGTCC TGTGGACAAG 
    AGATACGAGC CTCTGCCGCC AGTCAACCCT TCACCTGCTC CATACAGCCA GAACACACCT 
    GTCGCTCCGC CCACATCCCT GCCCAAACTC ACCAACATCG AGGTGAACTC TCTGCCAGAT 
    CCTCACAGCT CTCCCAAAGC CAAACCGGAC CTGTCTGCTC TGAGAGCTCC GACCCGGGAC 
    GAGCCCATCC AGAGCAGCTA CTTGCCCCCC AAGTCCACCA TCAATGGCAT GGATGCTCCT 
    CCCAAAACAC TCGGCGTGCC GACCAGCTAC AACCGCTACG TCCCCAAGCC TTACACCAGC 
    TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG AGTCCGAAAT TCAACCACAA CCTGCTTCCC 
    AACGACACGC AGTCCAAACC CAGTGTCAAC AACAACCTGA AGCCTCAGAT CTCCCCACAA 
    CCCCAGGACA CGGACAGCGG CGTCGACACC TTCACTCGCA CCATGGACAA CAGGCCAAAA 
    TATCAGCACA ACAACATCAA CGCCATTCCC AAAGCCGTCC CAGTCAGCCC CAGTGCACTG 
    GATGATGATG AAGAAGATGA GGGACACACA GTTGTGGCCA CGGCGCGGGG CATCTTTAAC 
    TGTAATGGCG GGGTGTTGAG CTCTATAGAG ACGGGCGTCA GCATCATTAT CCCACAGGGA 
    GCCATTCCTG ATAGTGTGGA GCAGGAGATC TACTTCAAAG TGTGCCGGGA CAACAGCATC 
    CTGCCTCCTC TGGATAAGGA GAAAGGTGAA ACTCTGCTGA GTCCGCTGGT GATGTGTGGT 
    CCTCACGGCC TGAAGTTCCT GAAGCCTGTG GAGCTGCGCT TGCCACACTG TGCGTCTATG 
    ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGACCCCAAA 
    TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC CCCAATTACC TGGTGGGCGC AAACTGTGTG 
    TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354178.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209667.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA.
    Price & Availability↑
    CloneID ODa57888
    Clone ID Related Accession (Same CDS sequence) XM_017354178.1
    Accession Version XM_017354178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGATGAGA CGGTAATATG GGAACAGCAC ACTGTAACTC TACACAGGGC TCCAGGGTTT 
    GGCTTCGGGA TCGCCATCTC CGGCGGACGG GACAACCCTC ACTTCCAGAG CGGGGAGACG 
    TCCATCGTCA TATCAGATGT GCTGAAGGGA GGCCCCGCCG AGGGCCTGCT GCAGGAGAAT 
    GACAGAGTGG TGATGGTCAA CGCCGTCTCC ATGGACAATG TGGATCACGC TTACGCAGTG 
    CAGCAGCTCC GCAAAAGTGG CAAGAATGCA AAAATAACCA TCAGGAGAAA GCGTAAGGTG 
    CAGATCCCGT CAGGCCGGCC GGGTGAACGC GAGACTATGT CAGAGCATGA CGAGGACGAC 
    GACAGCTACG AGGATGAGAT CTACGAGTCC CGGAGCGCGC GAAGCGGACC CAGCGCCTAC 
    AGTGGAGGTG GAGGCGGCGG CGGAGGTGGA GGAGCCTCGG GACGGAGGAG CGGCAGAGGC 
    GACAGGCTGA GTGGGAGGAG GGAGCGGGAC AGAGAGCGCA GCGGCTCCAG AGAGAGGAGT 
    CTGTCCCCGC GCTCGGACCG CCACTCTGTG TCCTCCAACC TCCCCCCACG GCCAGCCAAA 
    GTCACACTCG TCAAATCCCG CAAGAATGAA GCAGAATACG GGCTTCGCCT GGCCAGCCAC 
    ATCTTCGTGA AGGACATTTC CCCCGAGAGC CTGGCAGCCC GAGACGGAAA CATCCAAGAG 
    GGAGACGTGG TGCTTAAGAT TAATGGCACG GTAACAGAGA ATTTGTCCCT GATCGATGCT 
    AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
    GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
    AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
    CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
    AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
    CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
    CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
    CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
    GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
    TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
    GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
    AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
    ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
    ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
    TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
    AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
    CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
    AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
    CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
    AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
    CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
    AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
    GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
    ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
    TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
    AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
    GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
    ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
    GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
    GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
    CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
    CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
    TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
    CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
    GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
    GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
    GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
    CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
    AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
    CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
    CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
    GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
    CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
    TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
    CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
    CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
    AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
    GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
    GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
    CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
    CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
    CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
    CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
    GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
    ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
    TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
    AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
    CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
    GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
    GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
    GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
    ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
    GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
    ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
    TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
    AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
    CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
    CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
    GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
    GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
    GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
    AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
    CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
    CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
    GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
    TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209667.1
    CDS191..5518
    Translation

    Target ORF information:

    RefSeq Version XM_017354178.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGATGAGA CGGTAATATG GGAACAGCAC ACTGTAACTC TACACAGGGC TCCAGGGTTT 
    GGCTTCGGGA TCGCCATCTC CGGCGGACGG GACAACCCTC ACTTCCAGAG CGGGGAGACG 
    TCCATCGTCA TATCAGATGT GCTGAAGGGA GGCCCCGCCG AGGGCCTGCT GCAGGAGAAT 
    GACAGAGTGG TGATGGTCAA CGCCGTCTCC ATGGACAATG TGGATCACGC TTACGCAGTG 
    CAGCAGCTCC GCAAAAGTGG CAAGAATGCA AAAATAACCA TCAGGAGAAA GCGTAAGGTG 
    CAGATCCCGT CAGGCCGGCC GGGTGAACGC GAGACTATGT CAGAGCATGA CGAGGACGAC 
    GACAGCTACG AGGATGAGAT CTACGAGTCC CGGAGCGCGC GAAGCGGACC CAGCGCCTAC 
    AGTGGAGGTG GAGGCGGCGG CGGAGGTGGA GGAGCCTCGG GACGGAGGAG CGGCAGAGGC 
    GACAGGCTGA GTGGGAGGAG GGAGCGGGAC AGAGAGCGCA GCGGCTCCAG AGAGAGGAGT 
    CTGTCCCCGC GCTCGGACCG CCACTCTGTG TCCTCCAACC TCCCCCCACG GCCAGCCAAA 
    GTCACACTCG TCAAATCCCG CAAGAATGAA GCAGAATACG GGCTTCGCCT GGCCAGCCAC 
    ATCTTCGTGA AGGACATTTC CCCCGAGAGC CTGGCAGCCC GAGACGGAAA CATCCAAGAG 
    GGAGACGTGG TGCTTAAGAT TAATGGCACG GTAACAGAGA ATTTGTCCCT GATCGATGCT 
    AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
    GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
    AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
    CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
    AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
    CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
    CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
    CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
    GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
    TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
    GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
    AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
    ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
    ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
    TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
    AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
    CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
    AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
    CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
    AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
    CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
    AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
    GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
    ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
    TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
    AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
    GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
    ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
    GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
    GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
    CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
    CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
    TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
    CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
    GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
    GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
    GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
    CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
    AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
    CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
    CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
    GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
    CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
    TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
    CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
    CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
    AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
    GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
    GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
    CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
    CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
    CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
    CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
    GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
    ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
    TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
    AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
    CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
    GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
    GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
    GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
    ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
    GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
    ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
    TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
    AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
    CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
    CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
    GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
    GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
    GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
    AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
    CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
    CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
    GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
    TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470724.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326399.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa141490
    Clone ID Related Accession (Same CDS sequence) XM_021470724.1
    Accession Version XM_021470724.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGAATAC 
    GGGCTTCGCC TGGCCAGCCA CATCTTCGTG AAGGACATTT CCCCCGAGAG CCTGGCAGCC 
    CGAGACGGAA ACATCCAAGA GGGAGACGTG GTGCTTAAGA TTAATGGCAC GGTAACAGAG 
    AATTTGTCCC TGATCGATGC TAAGAAGCTG ATCGAGAGGT CAAAGGGAAA GCTGAAGATG 
    GTGGTGCAGA GAGACGAGCG GGCGACGCTG CTGAACATCC CCGATCTGGA CGACAGCATA 
    CCTTCTGCTA ATGCCTCGGA CAGAGATGAT ATTTCAGACA TCCACTCCGT GGCCTCTGAT 
    CATTCCAACC GATCCCACGA CCGGAAGAGG AGCAGCCGCT CTCGTTCTCC AGACCGCCGC 
    TCCGAACCCT CAGACCACTC CAGACACTCT CCACCACAGA TCAGCAACGG CAGGGCCACA 
    GGGCCGACGG CTCAGAGAAC GCGGCCAGTT CACAGGAGCC GTGATGAAGA CAGGATCTCC 
    AAGGCCGGGC CGCTTCCTCT ACCTGCTAAG ATGGTGGAGG AGATGCCGAA AGAGCAGAGC 
    GCAGCCAGAG AAACAGAGAA ACAGCTCCCC CCTCTGCCAG AGCCCAAACC TGTGTACGCT 
    CAGCCGGGCC AGCCGGACGT GGACCTGCCT GTTAGTCCGG CAGATGCTCC TGTGCCCAGC 
    GTCACACATG ATGACAGCAT CTTACGGCCG AGCATGAAGC TGGTGAAGTT CAAGAAGGGC 
    GAGAGCGTGG GCTTGAGGCT CGCTGGGGGG AACGACGTTG GGATATTTGT GGCGGGAGTT 
    CTGGAGGACA GTCCTGCTGC TAAAGAGGGC CTGGAAGAGG GAGACCAGAT TCTCAGGGTA 
    AATAATGTGG ATTTTGCAAA TATCATCCGA GAGGAGGCAG TGCTGTTCCT GCTGGATCTG 
    CCCAAAGGAG AGGAGGTCAC CATCCTGGCC CAGAAGAAGA AAGACGTGTA CCGCCGCATT 
    GTAGAGTCTG ACGTGGGAGA CTCGTTCTAC ATCAGGACTC ATTTTGAATA TGAGAAGGAG 
    TCGCCCTATG GCCTGAGCTT CAATAAGGGG GAGGTCTTCA GAGTCGTCGA CACCCTTTAC 
    AATGGCAAAC TGGGCTCCTG GCTCGCCATC CGCATCGGCA AAAACCACCA GGAGGTGGAG 
    CGGGGCATCA TCCCCAACAA GAACAGGGCT GAGCAGTTAT CAAGTGTCCA GTACACTCTC 
    CCAAAGACTC CAGGAGGGGA TCGCGCCGAC TTCTGGAGAT TCAGAGGCCT CCGCAGCTCC 
    AAACGAAACC TGAGGAAGAG CAGAGAAGAC CTGTCTGCTC AGCCGGTCCA AACCAAATTC 
    CCTGCTTATG AAAGAGTGGT GCTTAGAGAA GCTGGTTTCT TGAGGCCAGT GGTCATTTTC 
    GGACCGATCG CGGATGTGGC TAGAGAGAAG CTGTCCAGAG AGGAGACTGA CCTCTTCGAA 
    CTTGCAAAGA GTGAACCCAG AGATGCGGGC ACAGACCAGC GCAGCTCCGG AATCATCCGT 
    CTCCATACCA TTAAACAGAT CATTGACCGA GACAAGCACG CGGTGCTGGA CATCACCCCT 
    AATGCGGTGG ACCGTCTGAA CTACGCTCAG TGGTACCCCA TCGTGGTGTT TCTGAATCCA 
    GACAGCAAAC AGGGCGTCAA GAACATGAGG ACCAGGCTCT GTCCCGAGTC CAGGAAGAGC 
    GCCCGAAAGC TCTACGAGCG TGCTCTCAAA CTGAGGAAGA ACAACCATCA CCTTTTCACC 
    ACCACTATTA ACTTGAACAA CATGAATGAC GGGTGGTACG GTGCTTTGAA AGAGAGCATC 
    CAGCAGCAGC AGAACCAGTT GGTCTGGGTC TCAGAGGGCA AGGCCGACGG CGCTCCTGAC 
    GATGACCTTG ACATCCATGA CGACCGCCTC TCCTACCTCT CGGCTCCGGG CAGCGAGTAC 
    AGCATGTACA GCACCGACAG CCGCCACACG TCCGACTATG AGGACACCGA CACGGAGGGT 
    GGTGCCTACA CCGACCAGGA GCTGGACGAG ACCATGAACG ATGAGGTGGG CCTGCCTAGC 
    GAGCCTGCCA TCACACGCTC ATCTGAGCCT GTGCGAGAGG ACCCGCCAGT CATTCAGGAC 
    GCAGCTGGAT ACCCCAGCTA CCAGCATGCG GTACCGCAAC CTGAAGCGGT GAGCCGCATT 
    GACCCGGCCG GGTTTAAGAT GGCAGCGCCG CAGCAGCAAG CTGAGGCTGC TCTGGCCTCG 
    CCGCCCCCTC CCTCTGCAGC GGTCCAGCCC ACCGCAACAC TAGCGGGTAC GAACCCCGAG 
    GAGCCGCCTG GCACCCCTCA GGCCGACTCC CTTAACAGCC CCGTCCCTGT TCCCGACCCC 
    CAGCCCGAAC CTGAGCTCGC TCAGCCCCCA ACACACGACC CCCACCAGTC GCTTCCTCCT 
    GGCCCAGATC CAAAGATGTA TAAAAAGGAT CTGTACAGCA TGGACGAGCC GGTGCGAGTG 
    ACCCACGGCG TGAAGCCTCC CCCTCCCCAG CAGCAGCAGC AGCCTTTAGG CCCCCCAACC 
    TCGCTCTCCT ACAGCCACCA GCCTGTCTAC CAGGACCAAC AGCCATACCG CCAGTACGAC 
    CACCCGCCTT ATGGCTATGA CGGTGGCGGC TACGCACAAC CAAAGCCTCA CAACTACGAC 
    CCGCACCTGC ACTACGACAA CCGTGTGCCT CACTATAACG AGCAGTGGCC CACGTACGAC 
    CAGCAGCAGC AGCAGCAGAC TTCCCCCCAG CCGCCACCTG CGTCCGGCTT CCCTCAGGGC 
    CACCAGCCTC CGCCTCCACC ACCGATGGGA TATGAGCCAC GCTCCCCATA CGAGGACGGA 
    CCTAACAGGG ATTTCAGCCC TCCTCAGTCC CAGTATGACG GTGTGTCCCC AATGGGCTAC 
    GATCACCGGC CGCGGCACCC CAAACCTGCA CCGGTCCGAT ATGAAGAGCC TCCACCTCCA 
    CCTCCTCCGG TGTCCTACGA TGCACGGTCA CCTTTCGAAC CCGAGCCGCA TGCTTTCCCA 
    GGCAACACAC ATCGCTCGCC AGACCCACCG AAACAGTATT ATGGAGACGC CGCTATGAGG 
    CCCTTGTACA ACCCTGGACC TCCGAACAGA GTGTACAAAC ATGAGCCGGT GAACTCTGAG 
    CCTCCTGTAC CGCCTCCCAA ACCTGAATCT GTCCTGTCTC CAGGAGAACC GCCCCATTCT 
    GCAGCTTCCA AACCATTGCC GCCTCCTCCC AGAGATGATG ACGATGATGA TGACGATGAT 
    GATGACCCTG CCATGAAGCC CCAGTCTGTG CTCAACAGGG TCAAAATGTT CGAAAACAAA 
    CGCTCTGTGT CGGTAGACCG AGCCAAGGAC GCACCTGACG TCACTGGAAT TAGGCCCACA 
    GATCTCCCCA AACCTGCGAG CACCCCTGGA CCTGTGCTCA AAGCCAACTC TCTCAGCAAC 
    CTGGAGCAGG ACAAACCCTC CTACAGAGCT CCAGAGCCGC AGAAACCTCA GACGCGTGTT 
    GGGGACGATG TGGTTCGCTC CAACCACTAC GACCCTGATG ATGATGAAGA GTACTACAGA 
    AAACAGCTCT CATTTTTCGA CCGCCGCAGT TTCGACAGTA AGGTCATGAA TCCACCCAGC 
    GCTGGAGTAA ACCGCTTCCA TGAGCCGCCC AAACCACCCC AGACACAGAT CGCTTATCCT 
    TTCGCTAGGA CGGAGTCTGT GGAGAAGGTG AGTCCTGTGG ACAAGAGATA CGAGCCTCTG 
    CCGCCAGTCA ACCCTTCACC TGCTCCATAC AGCCAGAACA CACCTGTCGC TCCGCCCACA 
    TCCCTGCCCA AACTCACCAA CATCGAGGTG AACTCTCTGC CAGATCCTCA CAGCTCTCCC 
    AAAGCCAAAC CGGACCTGTC TGCTCTGAGA GCTCCGACCC GGGACGAGCC CATCCAGAGC 
    AGCTACTTGC CCCCCAAGTC CACCATCAAT GGCATGGATG CTCCTCCCAA AACACTCGGC 
    GTGCCGACCA GCTACAACCG CTACGTCCCC AAGCCTTACA CCAGCTCGGC GCGACCCTTC 
    GAGCGCAAGT TCGAGAGTCC GAAATTCAAC CACAACCTGC TTCCCAACGA CACGCAGTCC 
    AAACCCAGTG TCAACAACAA CCTGAAGCCT CAGATCTCCC CACAACCCCA GGACACGGAC 
    AGCGGCGTCG ACACCTTCAC TCGCACCATG GACAACAGGC CAAAATATCA GCACAACAAC 
    ATCAACGCCA TTCCCAAAGC CGTCCCAGTC AGCCCCAGTG CACTGGATGA TGATGAAGAA 
    GATGAGGGAC ACACAGTTGT GGCCACGGCG CGGGGCATCT TTAACTGTAA TGGCGGGGTG 
    TTGAGCTCTA TAGAGACGGG CGTCAGCATC ATTATCCCAC AGGGAGCCAT TCCTGATAGT 
    GTGGAGCAGG AGATCTACTT CAAAGTGTGC CGGGACAACA GCATCCTGCC TCCTCTGGAT 
    AAGGAGAAAG GTGAAACTCT GCTGAGTCCG CTGGTGATGT GTGGTCCTCA CGGCCTGAAG 
    TTCCTGAAGC CTGTGGAGCT GCGCTTGCCA CACTGTGCGT CTATGACCCC TGATGGTTGG 
    TCTTTTGCTC TAAAATCCTC CGACTCCTCG TCGGGTGACC CCAAATGCTG GCAAAACAAG 
    TGTCTACCAG GAGACCCCAA TTACCTGGTG GGCGCAAACT GTGTGTCTGT GCTGATCGAC 
    CACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326399.1
    CDS110..6238
    Translation

    Target ORF information:

    RefSeq Version XM_021470724.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470724.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGAATAC 
    GGGCTTCGCC TGGCCAGCCA CATCTTCGTG AAGGACATTT CCCCCGAGAG CCTGGCAGCC 
    CGAGACGGAA ACATCCAAGA GGGAGACGTG GTGCTTAAGA TTAATGGCAC GGTAACAGAG 
    AATTTGTCCC TGATCGATGC TAAGAAGCTG ATCGAGAGGT CAAAGGGAAA GCTGAAGATG 
    GTGGTGCAGA GAGACGAGCG GGCGACGCTG CTGAACATCC CCGATCTGGA CGACAGCATA 
    CCTTCTGCTA ATGCCTCGGA CAGAGATGAT ATTTCAGACA TCCACTCCGT GGCCTCTGAT 
    CATTCCAACC GATCCCACGA CCGGAAGAGG AGCAGCCGCT CTCGTTCTCC AGACCGCCGC 
    TCCGAACCCT CAGACCACTC CAGACACTCT CCACCACAGA TCAGCAACGG CAGGGCCACA 
    GGGCCGACGG CTCAGAGAAC GCGGCCAGTT CACAGGAGCC GTGATGAAGA CAGGATCTCC 
    AAGGCCGGGC CGCTTCCTCT ACCTGCTAAG ATGGTGGAGG AGATGCCGAA AGAGCAGAGC 
    GCAGCCAGAG AAACAGAGAA ACAGCTCCCC CCTCTGCCAG AGCCCAAACC TGTGTACGCT 
    CAGCCGGGCC AGCCGGACGT GGACCTGCCT GTTAGTCCGG CAGATGCTCC TGTGCCCAGC 
    GTCACACATG ATGACAGCAT CTTACGGCCG AGCATGAAGC TGGTGAAGTT CAAGAAGGGC 
    GAGAGCGTGG GCTTGAGGCT CGCTGGGGGG AACGACGTTG GGATATTTGT GGCGGGAGTT 
    CTGGAGGACA GTCCTGCTGC TAAAGAGGGC CTGGAAGAGG GAGACCAGAT TCTCAGGGTA 
    AATAATGTGG ATTTTGCAAA TATCATCCGA GAGGAGGCAG TGCTGTTCCT GCTGGATCTG 
    CCCAAAGGAG AGGAGGTCAC CATCCTGGCC CAGAAGAAGA AAGACGTGTA CCGCCGCATT 
    GTAGAGTCTG ACGTGGGAGA CTCGTTCTAC ATCAGGACTC ATTTTGAATA TGAGAAGGAG 
    TCGCCCTATG GCCTGAGCTT CAATAAGGGG GAGGTCTTCA GAGTCGTCGA CACCCTTTAC 
    AATGGCAAAC TGGGCTCCTG GCTCGCCATC CGCATCGGCA AAAACCACCA GGAGGTGGAG 
    CGGGGCATCA TCCCCAACAA GAACAGGGCT GAGCAGTTAT CAAGTGTCCA GTACACTCTC 
    CCAAAGACTC CAGGAGGGGA TCGCGCCGAC TTCTGGAGAT TCAGAGGCCT CCGCAGCTCC 
    AAACGAAACC TGAGGAAGAG CAGAGAAGAC CTGTCTGCTC AGCCGGTCCA AACCAAATTC 
    CCTGCTTATG AAAGAGTGGT GCTTAGAGAA GCTGGTTTCT TGAGGCCAGT GGTCATTTTC 
    GGACCGATCG CGGATGTGGC TAGAGAGAAG CTGTCCAGAG AGGAGACTGA CCTCTTCGAA 
    CTTGCAAAGA GTGAACCCAG AGATGCGGGC ACAGACCAGC GCAGCTCCGG AATCATCCGT 
    CTCCATACCA TTAAACAGAT CATTGACCGA GACAAGCACG CGGTGCTGGA CATCACCCCT 
    AATGCGGTGG ACCGTCTGAA CTACGCTCAG TGGTACCCCA TCGTGGTGTT TCTGAATCCA 
    GACAGCAAAC AGGGCGTCAA GAACATGAGG ACCAGGCTCT GTCCCGAGTC CAGGAAGAGC 
    GCCCGAAAGC TCTACGAGCG TGCTCTCAAA CTGAGGAAGA ACAACCATCA CCTTTTCACC 
    ACCACTATTA ACTTGAACAA CATGAATGAC GGGTGGTACG GTGCTTTGAA AGAGAGCATC 
    CAGCAGCAGC AGAACCAGTT GGTCTGGGTC TCAGAGGGCA AGGCCGACGG CGCTCCTGAC 
    GATGACCTTG ACATCCATGA CGACCGCCTC TCCTACCTCT CGGCTCCGGG CAGCGAGTAC 
    AGCATGTACA GCACCGACAG CCGCCACACG TCCGACTATG AGGACACCGA CACGGAGGGT 
    GGTGCCTACA CCGACCAGGA GCTGGACGAG ACCATGAACG ATGAGGTGGG CCTGCCTAGC 
    GAGCCTGCCA TCACACGCTC ATCTGAGCCT GTGCGAGAGG ACCCGCCAGT CATTCAGGAC 
    GCAGCTGGAT ACCCCAGCTA CCAGCATGCG GTACCGCAAC CTGAAGCGGT GAGCCGCATT 
    GACCCGGCCG GGTTTAAGAT GGCAGCGCCG CAGCAGCAAG CTGAGGCTGC TCTGGCCTCG 
    CCGCCCCCTC CCTCTGCAGC GGTCCAGCCC ACCGCAACAC TAGCGGGTAC GAACCCCGAG 
    GAGCCGCCTG GCACCCCTCA GGCCGACTCC CTTAACAGCC CCGTCCCTGT TCCCGACCCC 
    CAGCCCGAAC CTGAGCTCGC TCAGCCCCCA ACACACGACC CCCACCAGTC GCTTCCTCCT 
    GGCCCAGATC CAAAGATGTA TAAAAAGGAT CTGTACAGCA TGGACGAGCC GGTGCGAGTG 
    ACCCACGGCG TGAAGCCTCC CCCTCCCCAG CAGCAGCAGC AGCCTTTAGG CCCCCCAACC 
    TCGCTCTCCT ACAGCCACCA GCCTGTCTAC CAGGACCAAC AGCCATACCG CCAGTACGAC 
    CACCCGCCTT ATGGCTATGA CGGTGGCGGC TACGCACAAC CAAAGCCTCA CAACTACGAC 
    CCGCACCTGC ACTACGACAA CCGTGTGCCT CACTATAACG AGCAGTGGCC CACGTACGAC 
    CAGCAGCAGC AGCAGCAGAC TTCCCCCCAG CCGCCACCTG CGTCCGGCTT CCCTCAGGGC 
    CACCAGCCTC CGCCTCCACC ACCGATGGGA TATGAGCCAC GCTCCCCATA CGAGGACGGA 
    CCTAACAGGG ATTTCAGCCC TCCTCAGTCC CAGTATGACG GTGTGTCCCC AATGGGCTAC 
    GATCACCGGC CGCGGCACCC CAAACCTGCA CCGGTCCGAT ATGAAGAGCC TCCACCTCCA 
    CCTCCTCCGG TGTCCTACGA TGCACGGTCA CCTTTCGAAC CCGAGCCGCA TGCTTTCCCA 
    GGCAACACAC ATCGCTCGCC AGACCCACCG AAACAGTATT ATGGAGACGC CGCTATGAGG 
    CCCTTGTACA ACCCTGGACC TCCGAACAGA GTGTACAAAC ATGAGCCGGT GAACTCTGAG 
    CCTCCTGTAC CGCCTCCCAA ACCTGAATCT GTCCTGTCTC CAGGAGAACC GCCCCATTCT 
    GCAGCTTCCA AACCATTGCC GCCTCCTCCC AGAGATGATG ACGATGATGA TGACGATGAT 
    GATGACCCTG CCATGAAGCC CCAGTCTGTG CTCAACAGGG TCAAAATGTT CGAAAACAAA 
    CGCTCTGTGT CGGTAGACCG AGCCAAGGAC GCACCTGACG TCACTGGAAT TAGGCCCACA 
    GATCTCCCCA AACCTGCGAG CACCCCTGGA CCTGTGCTCA AAGCCAACTC TCTCAGCAAC 
    CTGGAGCAGG ACAAACCCTC CTACAGAGCT CCAGAGCCGC AGAAACCTCA GACGCGTGTT 
    GGGGACGATG TGGTTCGCTC CAACCACTAC GACCCTGATG ATGATGAAGA GTACTACAGA 
    AAACAGCTCT CATTTTTCGA CCGCCGCAGT TTCGACAGTA AGGTCATGAA TCCACCCAGC 
    GCTGGAGTAA ACCGCTTCCA TGAGCCGCCC AAACCACCCC AGACACAGAT CGCTTATCCT 
    TTCGCTAGGA CGGAGTCTGT GGAGAAGGTG AGTCCTGTGG ACAAGAGATA CGAGCCTCTG 
    CCGCCAGTCA ACCCTTCACC TGCTCCATAC AGCCAGAACA CACCTGTCGC TCCGCCCACA 
    TCCCTGCCCA AACTCACCAA CATCGAGGTG AACTCTCTGC CAGATCCTCA CAGCTCTCCC 
    AAAGCCAAAC CGGACCTGTC TGCTCTGAGA GCTCCGACCC GGGACGAGCC CATCCAGAGC 
    AGCTACTTGC CCCCCAAGTC CACCATCAAT GGCATGGATG CTCCTCCCAA AACACTCGGC 
    GTGCCGACCA GCTACAACCG CTACGTCCCC AAGCCTTACA CCAGCTCGGC GCGACCCTTC 
    GAGCGCAAGT TCGAGAGTCC GAAATTCAAC CACAACCTGC TTCCCAACGA CACGCAGTCC 
    AAACCCAGTG TCAACAACAA CCTGAAGCCT CAGATCTCCC CACAACCCCA GGACACGGAC 
    AGCGGCGTCG ACACCTTCAC TCGCACCATG GACAACAGGC CAAAATATCA GCACAACAAC 
    ATCAACGCCA TTCCCAAAGC CGTCCCAGTC AGCCCCAGTG CACTGGATGA TGATGAAGAA 
    GATGAGGGAC ACACAGTTGT GGCCACGGCG CGGGGCATCT TTAACTGTAA TGGCGGGGTG 
    TTGAGCTCTA TAGAGACGGG CGTCAGCATC ATTATCCCAC AGGGAGCCAT TCCTGATAGT 
    GTGGAGCAGG AGATCTACTT CAAAGTGTGC CGGGACAACA GCATCCTGCC TCCTCTGGAT 
    AAGGAGAAAG GTGAAACTCT GCTGAGTCCG CTGGTGATGT GTGGTCCTCA CGGCCTGAAG 
    TTCCTGAAGC CTGTGGAGCT GCGCTTGCCA CACTGTGCGT CTATGACCCC TGATGGTTGG 
    TCTTTTGCTC TAAAATCCTC CGACTCCTCG TCGGGTGACC CCAAATGCTG GCAAAACAAG 
    TGTCTACCAG GAGACCCCAA TTACCTGGTG GGCGCAAACT GTGTGTCTGT GCTGATCGAC 
    CACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470731.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326406.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID ODa141491
    Clone ID Related Accession (Same CDS sequence) XM_021470731.1
    Accession Version XM_021470731.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGAAGCTGAT CGAGAGGTCA 
    AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
    GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGACAT CCACTCCGTG 
    GCCTCTGATC ATTCCAACCG ATCCCACGAC CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA 
    GACCGCCGCT CCGAACCCTC AGACCACTCC AGACACTCTC CACCACAGAT CAGCAACGGC 
    AGGGCCACAG GGCCGACGGC TCAGAGAACG CGGCCAGTTC ACAGGAGCCG TGATGAAGAC 
    AGGATCTCCA AGGCCGGGCC GCTTCCTCTA CCTGCTAAGA TGGTGGAGGA GATGCCGAAA 
    GAGCAGAGCG CAGCCAGAGA AACAGAGAAA CAGCTCCCCC CTCTGCCAGA GCCCAAACCT 
    GTGTACGCTC AGCCGGGCCA GCCGGACGTG GACCTGCCTG TTAGTCCGGC AGATGCTCCT 
    GTGCCCAGCG TCACACATGA TGACAGCATC TTACGGCCGA GCATGAAGCT GGTGAAGTTC 
    AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC GCTGGGGGGA ACGACGTTGG GATATTTGTG 
    GCGGGAGTTC TGGAGGACAG TCCTGCTGCT AAAGAGGGCC TGGAAGAGGG AGACCAGATT 
    CTCAGGGTAA ATAATGTGGA TTTTGCAAAT ATCATCCGAG AGGAGGCAGT GCTGTTCCTG 
    CTGGATCTGC CCAAAGGAGA GGAGGTCACC ATCCTGGCCC AGAAGAAGAA AGACGTGTAC 
    CGCCGCATTG TAGAGTCTGA CGTGGGAGAC TCGTTCTACA TCAGGACTCA TTTTGAATAT 
    GAGAAGGAGT CGCCCTATGG CCTGAGCTTC AATAAGGGGG AGGTCTTCAG AGTCGTCGAC 
    ACCCTTTACA ATGGCAAACT GGGCTCCTGG CTCGCCATCC GCATCGGCAA AAACCACCAG 
    GAGGTGGAGC GGGGCATCAT CCCCAACAAG AACAGGGCTG AGCAGTTATC AAGTGTCCAG 
    TACACTCTCC CAAAGACTCC AGGAGGGGAT CGCGCCGACT TCTGGAGATT CAGAGGCCTC 
    CGCAGCTCCA AACGAAACCT GAGGAAGAGC AGAGAAGACC TGTCTGCTCA GCCGGTCCAA 
    ACCAAATTCC CTGCTTATGA AAGAGTGGTG CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG 
    GTCATTTTCG GACCGATCGC GGATGTGGCT AGAGAGAAGC TGTCCAGAGA GGAGACTGAC 
    CTCTTCGAAC TTGCAAAGAG TGAACCCAGA GATGCGGGCA CAGACCAGCG CAGCTCCGGA 
    ATCATCCGTC TCCATACCAT TAAACAGATC ATTGACCGAG ACAAGCACGC GGTGCTGGAC 
    ATCACCCCTA ATGCGGTGGA CCGTCTGAAC TACGCTCAGT GGTACCCCAT CGTGGTGTTT 
    CTGAATCCAG ACAGCAAACA GGGCGTCAAG AACATGAGGA CCAGGCTCTG TCCCGAGTCC 
    AGGAAGAGCG CCCGAAAGCT CTACGAGCGT GCTCTCAAAC TGAGGAAGAA CAACCATCAC 
    CTTTTCACCA CCACTATTAA CTTGAACAAC ATGAATGACG GGTGGTACGG TGCTTTGAAA 
    GAGAGCATCC AGCAGCAGCA GAACCAGTTG GTCTGGGTCT CAGAGGGCAA GGCCGACGGC 
    GCTCCTGACG ATGACCTTGA CATCCATGAC GACCGCCTCT CCTACCTCTC GGCTCCGGGC 
    AGCGAGTACA GCATGTACAG CACCGACAGC CGCCACACGT CCGACTATGA GGACACCGAC 
    ACGGAGGGTG GTGCCTACAC CGACCAGGAG CTGGACGAGA CCATGAACGA TGAGGTGGGC 
    CTGCCTAGCG AGCCTGCCAT CACACGCTCA TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC 
    ATTCAGGACG CAGCTGGATA CCCCAGCTAC CAGCATGCGG TACCGCAACC TGAAGCGGTG 
    AGCCGCATTG ACCCGGCCGG GTTTAAGATG GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT 
    CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG GTCCAGCCCA CCGCAACACT AGCGGGTACG 
    AACCCCGAGG AGCCGCCTGG CACCCCTCAG GCCGACTCCC TTAACAGCCC CGTCCCTGTT 
    CCCGACCCCC AGCCCGAACC TGAGCTCGCT CAGCCCCCAA CACACGACCC CCACCAGTCG 
    CTTCCTCCTG GCCCAGATCC AAAGATGTAT AAAAAGGATC TGTACAGCAT GGACGAGCCG 
    GTGCGAGTGA CCCACGGCGT GAAGCCTCCC CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC 
    CCCCCAACCT CGCTCTCCTA CAGCCACCAG CCTGTCTACC AGGACCAACA GCCATACCGC 
    CAGTACGACC ACCCGCCTTA TGGCTATGAC GGTGGCGGCT ACGCACAACC AAAGCCTCAC 
    AACTACGACC CGCACCTGCA CTACGACAAC CGTGTGCCTC ACTATAACGA GCAGTGGCCC 
    ACGTACGACC AGCAGCAGCA GCAGCAGACT TCCCCCCAGC CGCCACCTGC GTCCGGCTTC 
    CCTCAGGGCC ACCAGCCTCC GCCTCCACCA CCGATGGGAT ATGAGCCACG CTCCCCATAC 
    GAGGACGGAC CTAACAGGGA TTTCAGCCCT CCTCAGTCCC AGTATGACGG TGTGTCCCCA 
    ATGGGCTACG ATCACCGGCC GCGGCACCCC AAACCTGCAC CGGTCCGATA TGAAGAGCCT 
    CCACCTCCAC CTCCTCCGGT GTCCTACGAT GCACGGTCAC CTTTCGAACC CGAGCCGCAT 
    GCTTTCCCAG GCAACACACA TCGCTCGCCA GACCCACCGA AACAGTATTA TGGAGACGCC 
    GCTATGAGGC CCTTGTACAA CCCTGGACCT CCGAACAGAG TGTACAAACA TGAGCCGGTG 
    AACTCTGAGC CTCCTGTACC GCCTCCCAAA CCTGAATCTG TCCTGTCTCC AGGAGAACCG 
    CCCCATTCTG CAGCTTCCAA ACCATTGCCG CCTCCTCCCA GAGATGATGA CGATGATGAT 
    GACGATGATG ATGACCCTGC CATGAAGCCC CAGTCTGTGC TCAACAGGGT CAAAATGTTC 
    GAAAACAAAC GCTCTGTGTC GGTAGACCGA GCCAAGGACG CACCTGACGT CACTGGAATT 
    AGGCCCACAG ATCTCCCCAA ACCTGCGAGC ACCCCTGGAC CTGTGCTCAA AGCCAACTCT 
    CTCAGCAACC TGGAGCAGGA CAAACCCTCC TACAGAGCTC CAGAGCCGCA GAAACCTCAG 
    ACGCGTGTTG GGGACGATGT GGTTCGCTCC AACCACTACG ACCCTGATGA TGATGAAGAG 
    TACTACAGAA AACAGCTCTC ATTTTTCGAC CGCCGCAGTT TCGACAGTAA GGTCATGAAT 
    CCACCCAGCG CTGGAGTAAA CCGCTTCCAT GAGCCGCCCA AACCACCCCA GACACAGATC 
    GCTTATCCTT TCGCTAGGAC GGAGTCTGTG GAGAAGGTGA GTCCTGTGGA CAAGAGATAC 
    GAGCCTCTGC CGCCAGTCAA CCCTTCACCT GCTCCATACA GCCAGAACAC ACCTGTCGCT 
    CCGCCCACAT CCCTGCCCAA ACTCACCAAC ATCGAGGTGA ACTCTCTGCC AGATCCTCAC 
    AGCTCTCCCA AAGCCAAACC GGACCTGTCT GCTCTGAGAG CTCCGACCCG GGACGAGCCC 
    ATCCAGAGCA GCTACTTGCC CCCCAAGTCC ACCATCAATG GCATGGATGC TCCTCCCAAA 
    ACACTCGGCG TGCCGACCAG CTACAACCGC TACGTCCCCA AGCCTTACAC CAGCTCGGCG 
    CGACCCTTCG AGCGCAAGTT CGAGAGTCCG AAATTCAACC ACAACCTGCT TCCCAACGAC 
    ACGCAGTCCA AACCCAGTGT CAACAACAAC CTGAAGCCTC AGATCTCCCC ACAACCCCAG 
    GACACGGACA GCGGCGTCGA CACCTTCACT CGCACCATGG ACAACAGGCC AAAATATCAG 
    CACAACAACA TCAACGCCAT TCCCAAAGCC GTCCCAGTCA GCCCCAGTGC ACTGGATGAT 
    GATGAAGAAG ATGAGGGACA CACAGTTGTG GCCACGGCGC GGGGCATCTT TAACTGTAAT 
    GGCGGGGTGT TGAGCTCTAT AGAGACGGGC GTCAGCATCA TTATCCCACA GGGAGCCATT 
    CCTGATAGTG TGGAGCAGGA GATCTACTTC AAAGTGTGCC GGGACAACAG CATCCTGCCT 
    CCTCTGGATA AGGAGAAAGG TGAAACTCTG CTGAGTCCGC TGGTGATGTG TGGTCCTCAC 
    GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG CGCTTGCCAC ACTGTGCGTC TATGACCCCT 
    GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGACCC CAAATGCTGG 
    CAAAACAAGT GTCTACCAGG AGACCCCAAT TACCTGGTGG GCGCAAACTG TGTGTCTGTG 
    CTGATCGACC ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326406.1
    CDS110..6187
    Translation

    Target ORF information:

    RefSeq Version XM_021470731.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470731.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGAAGCTGAT CGAGAGGTCA 
    AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
    GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGACAT CCACTCCGTG 
    GCCTCTGATC ATTCCAACCG ATCCCACGAC CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA 
    GACCGCCGCT CCGAACCCTC AGACCACTCC AGACACTCTC CACCACAGAT CAGCAACGGC 
    AGGGCCACAG GGCCGACGGC TCAGAGAACG CGGCCAGTTC ACAGGAGCCG TGATGAAGAC 
    AGGATCTCCA AGGCCGGGCC GCTTCCTCTA CCTGCTAAGA TGGTGGAGGA GATGCCGAAA 
    GAGCAGAGCG CAGCCAGAGA AACAGAGAAA CAGCTCCCCC CTCTGCCAGA GCCCAAACCT 
    GTGTACGCTC AGCCGGGCCA GCCGGACGTG GACCTGCCTG TTAGTCCGGC AGATGCTCCT 
    GTGCCCAGCG TCACACATGA TGACAGCATC TTACGGCCGA GCATGAAGCT GGTGAAGTTC 
    AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC GCTGGGGGGA ACGACGTTGG GATATTTGTG 
    GCGGGAGTTC TGGAGGACAG TCCTGCTGCT AAAGAGGGCC TGGAAGAGGG AGACCAGATT 
    CTCAGGGTAA ATAATGTGGA TTTTGCAAAT ATCATCCGAG AGGAGGCAGT GCTGTTCCTG 
    CTGGATCTGC CCAAAGGAGA GGAGGTCACC ATCCTGGCCC AGAAGAAGAA AGACGTGTAC 
    CGCCGCATTG TAGAGTCTGA CGTGGGAGAC TCGTTCTACA TCAGGACTCA TTTTGAATAT 
    GAGAAGGAGT CGCCCTATGG CCTGAGCTTC AATAAGGGGG AGGTCTTCAG AGTCGTCGAC 
    ACCCTTTACA ATGGCAAACT GGGCTCCTGG CTCGCCATCC GCATCGGCAA AAACCACCAG 
    GAGGTGGAGC GGGGCATCAT CCCCAACAAG AACAGGGCTG AGCAGTTATC AAGTGTCCAG 
    TACACTCTCC CAAAGACTCC AGGAGGGGAT CGCGCCGACT TCTGGAGATT CAGAGGCCTC 
    CGCAGCTCCA AACGAAACCT GAGGAAGAGC AGAGAAGACC TGTCTGCTCA GCCGGTCCAA 
    ACCAAATTCC CTGCTTATGA AAGAGTGGTG CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG 
    GTCATTTTCG GACCGATCGC GGATGTGGCT AGAGAGAAGC TGTCCAGAGA GGAGACTGAC 
    CTCTTCGAAC TTGCAAAGAG TGAACCCAGA GATGCGGGCA CAGACCAGCG CAGCTCCGGA 
    ATCATCCGTC TCCATACCAT TAAACAGATC ATTGACCGAG ACAAGCACGC GGTGCTGGAC 
    ATCACCCCTA ATGCGGTGGA CCGTCTGAAC TACGCTCAGT GGTACCCCAT CGTGGTGTTT 
    CTGAATCCAG ACAGCAAACA GGGCGTCAAG AACATGAGGA CCAGGCTCTG TCCCGAGTCC 
    AGGAAGAGCG CCCGAAAGCT CTACGAGCGT GCTCTCAAAC TGAGGAAGAA CAACCATCAC 
    CTTTTCACCA CCACTATTAA CTTGAACAAC ATGAATGACG GGTGGTACGG TGCTTTGAAA 
    GAGAGCATCC AGCAGCAGCA GAACCAGTTG GTCTGGGTCT CAGAGGGCAA GGCCGACGGC 
    GCTCCTGACG ATGACCTTGA CATCCATGAC GACCGCCTCT CCTACCTCTC GGCTCCGGGC 
    AGCGAGTACA GCATGTACAG CACCGACAGC CGCCACACGT CCGACTATGA GGACACCGAC 
    ACGGAGGGTG GTGCCTACAC CGACCAGGAG CTGGACGAGA CCATGAACGA TGAGGTGGGC 
    CTGCCTAGCG AGCCTGCCAT CACACGCTCA TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC 
    ATTCAGGACG CAGCTGGATA CCCCAGCTAC CAGCATGCGG TACCGCAACC TGAAGCGGTG 
    AGCCGCATTG ACCCGGCCGG GTTTAAGATG GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT 
    CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG GTCCAGCCCA CCGCAACACT AGCGGGTACG 
    AACCCCGAGG AGCCGCCTGG CACCCCTCAG GCCGACTCCC TTAACAGCCC CGTCCCTGTT 
    CCCGACCCCC AGCCCGAACC TGAGCTCGCT CAGCCCCCAA CACACGACCC CCACCAGTCG 
    CTTCCTCCTG GCCCAGATCC AAAGATGTAT AAAAAGGATC TGTACAGCAT GGACGAGCCG 
    GTGCGAGTGA CCCACGGCGT GAAGCCTCCC CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC 
    CCCCCAACCT CGCTCTCCTA CAGCCACCAG CCTGTCTACC AGGACCAACA GCCATACCGC 
    CAGTACGACC ACCCGCCTTA TGGCTATGAC GGTGGCGGCT ACGCACAACC AAAGCCTCAC 
    AACTACGACC CGCACCTGCA CTACGACAAC CGTGTGCCTC ACTATAACGA GCAGTGGCCC 
    ACGTACGACC AGCAGCAGCA GCAGCAGACT TCCCCCCAGC CGCCACCTGC GTCCGGCTTC 
    CCTCAGGGCC ACCAGCCTCC GCCTCCACCA CCGATGGGAT ATGAGCCACG CTCCCCATAC 
    GAGGACGGAC CTAACAGGGA TTTCAGCCCT CCTCAGTCCC AGTATGACGG TGTGTCCCCA 
    ATGGGCTACG ATCACCGGCC GCGGCACCCC AAACCTGCAC CGGTCCGATA TGAAGAGCCT 
    CCACCTCCAC CTCCTCCGGT GTCCTACGAT GCACGGTCAC CTTTCGAACC CGAGCCGCAT 
    GCTTTCCCAG GCAACACACA TCGCTCGCCA GACCCACCGA AACAGTATTA TGGAGACGCC 
    GCTATGAGGC CCTTGTACAA CCCTGGACCT CCGAACAGAG TGTACAAACA TGAGCCGGTG 
    AACTCTGAGC CTCCTGTACC GCCTCCCAAA CCTGAATCTG TCCTGTCTCC AGGAGAACCG 
    CCCCATTCTG CAGCTTCCAA ACCATTGCCG CCTCCTCCCA GAGATGATGA CGATGATGAT 
    GACGATGATG ATGACCCTGC CATGAAGCCC CAGTCTGTGC TCAACAGGGT CAAAATGTTC 
    GAAAACAAAC GCTCTGTGTC GGTAGACCGA GCCAAGGACG CACCTGACGT CACTGGAATT 
    AGGCCCACAG ATCTCCCCAA ACCTGCGAGC ACCCCTGGAC CTGTGCTCAA AGCCAACTCT 
    CTCAGCAACC TGGAGCAGGA CAAACCCTCC TACAGAGCTC CAGAGCCGCA GAAACCTCAG 
    ACGCGTGTTG GGGACGATGT GGTTCGCTCC AACCACTACG ACCCTGATGA TGATGAAGAG 
    TACTACAGAA AACAGCTCTC ATTTTTCGAC CGCCGCAGTT TCGACAGTAA GGTCATGAAT 
    CCACCCAGCG CTGGAGTAAA CCGCTTCCAT GAGCCGCCCA AACCACCCCA GACACAGATC 
    GCTTATCCTT TCGCTAGGAC GGAGTCTGTG GAGAAGGTGA GTCCTGTGGA CAAGAGATAC 
    GAGCCTCTGC CGCCAGTCAA CCCTTCACCT GCTCCATACA GCCAGAACAC ACCTGTCGCT 
    CCGCCCACAT CCCTGCCCAA ACTCACCAAC ATCGAGGTGA ACTCTCTGCC AGATCCTCAC 
    AGCTCTCCCA AAGCCAAACC GGACCTGTCT GCTCTGAGAG CTCCGACCCG GGACGAGCCC 
    ATCCAGAGCA GCTACTTGCC CCCCAAGTCC ACCATCAATG GCATGGATGC TCCTCCCAAA 
    ACACTCGGCG TGCCGACCAG CTACAACCGC TACGTCCCCA AGCCTTACAC CAGCTCGGCG 
    CGACCCTTCG AGCGCAAGTT CGAGAGTCCG AAATTCAACC ACAACCTGCT TCCCAACGAC 
    ACGCAGTCCA AACCCAGTGT CAACAACAAC CTGAAGCCTC AGATCTCCCC ACAACCCCAG 
    GACACGGACA GCGGCGTCGA CACCTTCACT CGCACCATGG ACAACAGGCC AAAATATCAG 
    CACAACAACA TCAACGCCAT TCCCAAAGCC GTCCCAGTCA GCCCCAGTGC ACTGGATGAT 
    GATGAAGAAG ATGAGGGACA CACAGTTGTG GCCACGGCGC GGGGCATCTT TAACTGTAAT 
    GGCGGGGTGT TGAGCTCTAT AGAGACGGGC GTCAGCATCA TTATCCCACA GGGAGCCATT 
    CCTGATAGTG TGGAGCAGGA GATCTACTTC AAAGTGTGCC GGGACAACAG CATCCTGCCT 
    CCTCTGGATA AGGAGAAAGG TGAAACTCTG CTGAGTCCGC TGGTGATGTG TGGTCCTCAC 
    GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG CGCTTGCCAC ACTGTGCGTC TATGACCCCT 
    GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGACCC CAAATGCTGG 
    CAAAACAAGT GTCTACCAGG AGACCCCAAT TACCTGGTGG GCGCAAACTG TGTGTCTGTG 
    CTGATCGACC ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470723.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326398.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa44616
    Clone ID Related Accession (Same CDS sequence) XM_021470723.1
    Accession Version XM_021470723.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
    ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
    TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
    AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
    GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
    AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
    GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
    GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
    GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
    CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
    ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
    GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
    TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
    GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
    CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
    TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
    TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
    TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
    GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
    CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
    CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
    CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
    AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
    ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
    ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
    GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
    TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
    GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
    AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
    GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
    ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
    TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
    GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
    CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
    CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
    GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
    ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
    GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
    GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
    GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
    GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
    GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
    TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
    CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
    CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
    AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
    GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
    TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
    GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
    AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
    ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
    AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
    GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
    AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
    AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
    CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
    CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
    ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
    CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
    AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
    GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
    TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
    TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
    GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
    AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
    GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
    GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
    AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
    GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
    AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG CGTCTATGAC CCCTGATGGT 
    TGGTCTTTTG CTCTAAAATC CTCCGACTCC TCGTCGGGTG ACCCCAAATG CTGGCAAAAC 
    AAGTGTCTAC CAGGAGACCC CAATTACCTG GTGGGCGCAA ACTGTGTGTC TGTGCTGATC 
    GACCACTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326398.1
    CDS110..6241
    Translation

    Target ORF information:

    RefSeq Version XM_021470723.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470723.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
    ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
    TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
    AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
    GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
    AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
    GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
    GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
    GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
    CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
    ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
    GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
    TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
    GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
    CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
    TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
    TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
    TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
    GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
    CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
    CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
    CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
    AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
    ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
    ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
    GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
    TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
    GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
    AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
    GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
    ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
    TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
    GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
    CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
    CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
    GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
    ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
    GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
    GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
    GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
    GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
    GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
    TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
    CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
    CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
    AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
    GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
    TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
    GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
    AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
    ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
    AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
    GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
    AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
    AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
    CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
    CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
    ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
    CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
    AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
    GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
    TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
    TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
    GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
    AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
    GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
    GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
    AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
    GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
    AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG CGTCTATGAC CCCTGATGGT 
    TGGTCTTTTG CTCTAAAATC CTCCGACTCC TCGTCGGGTG ACCCCAAATG CTGGCAAAAC 
    AAGTGTCTAC CAGGAGACCC CAATTACCTG GTGGGCGCAA ACTGTGTGTC TGTGCTGATC 
    GACCACTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470725.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326400.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa44617
    Clone ID Related Accession (Same CDS sequence) XM_021470725.1
    Accession Version XM_021470725.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6123bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GACATCCACT CCGTGGCCTC TGATCATTCC 
    AACCGATCCC ACGACCGGAA GAGGAGCAGC CGCTCTCGTT CTCCAGACCG CCGCTCCGAA 
    CCCTCAGACC ACTCCAGACA CTCTCCACCA CAGATCAGCA ACGGCAGGGC CACAGGGCCG 
    ACGGCTCAGA GAACGCGGCC AGTTCACAGG AGCCGTGATG AAGACAGGAT CTCCAAGGCC 
    GGGCCGCTTC CTCTACCTGC TAAGATGGTG GAGGAGATGC CGAAAGAGCA GAGCGCAGCC 
    AGAGAAACAG AGAAACAGCT CCCCCCTCTG CCAGAGCCCA AACCTGTGTA CGCTCAGCCG 
    GGCCAGCCGG ACGTGGACCT GCCTGTTAGT CCGGCAGATG CTCCTGTGCC CAGCGTCACA 
    CATGATGACA GCATCTTACG GCCGAGCATG AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC 
    GTGGGCTTGA GGCTCGCTGG GGGGAACGAC GTTGGGATAT TTGTGGCGGG AGTTCTGGAG 
    GACAGTCCTG CTGCTAAAGA GGGCCTGGAA GAGGGAGACC AGATTCTCAG GGTAAATAAT 
    GTGGATTTTG CAAATATCAT CCGAGAGGAG GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA 
    GGAGAGGAGG TCACCATCCT GGCCCAGAAG AAGAAAGACG TGTACCGCCG CATTGTAGAG 
    TCTGACGTGG GAGACTCGTT CTACATCAGG ACTCATTTTG AATATGAGAA GGAGTCGCCC 
    TATGGCCTGA GCTTCAATAA GGGGGAGGTC TTCAGAGTCG TCGACACCCT TTACAATGGC 
    AAACTGGGCT CCTGGCTCGC CATCCGCATC GGCAAAAACC ACCAGGAGGT GGAGCGGGGC 
    ATCATCCCCA ACAAGAACAG GGCTGAGCAG TTATCAAGTG TCCAGTACAC TCTCCCAAAG 
    ACTCCAGGAG GGGATCGCGC CGACTTCTGG AGATTCAGAG GCCTCCGCAG CTCCAAACGA 
    AACCTGAGGA AGAGCAGAGA AGACCTGTCT GCTCAGCCGG TCCAAACCAA ATTCCCTGCT 
    TATGAAAGAG TGGTGCTTAG AGAAGCTGGT TTCTTGAGGC CAGTGGTCAT TTTCGGACCG 
    ATCGCGGATG TGGCTAGAGA GAAGCTGTCC AGAGAGGAGA CTGACCTCTT CGAACTTGCA 
    AAGAGTGAAC CCAGAGATGC GGGCACAGAC CAGCGCAGCT CCGGAATCAT CCGTCTCCAT 
    ACCATTAAAC AGATCATTGA CCGAGACAAG CACGCGGTGC TGGACATCAC CCCTAATGCG 
    GTGGACCGTC TGAACTACGC TCAGTGGTAC CCCATCGTGG TGTTTCTGAA TCCAGACAGC 
    AAACAGGGCG TCAAGAACAT GAGGACCAGG CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA 
    AAGCTCTACG AGCGTGCTCT CAAACTGAGG AAGAACAACC ATCACCTTTT CACCACCACT 
    ATTAACTTGA ACAACATGAA TGACGGGTGG TACGGTGCTT TGAAAGAGAG CATCCAGCAG 
    CAGCAGAACC AGTTGGTCTG GGTCTCAGAG GGCAAGGCCG ACGGCGCTCC TGACGATGAC 
    CTTGACATCC ATGACGACCG CCTCTCCTAC CTCTCGGCTC CGGGCAGCGA GTACAGCATG 
    TACAGCACCG ACAGCCGCCA CACGTCCGAC TATGAGGACA CCGACACGGA GGGTGGTGCC 
    TACACCGACC AGGAGCTGGA CGAGACCATG AACGATGAGG TGGGCCTGCC TAGCGAGCCT 
    GCCATCACAC GCTCATCTGA GCCTGTGCGA GAGGACCCGC CAGTCATTCA GGACGCAGCT 
    GGATACCCCA GCTACCAGCA TGCGGTACCG CAACCTGAAG CGGTGAGCCG CATTGACCCG 
    GCCGGGTTTA AGATGGCAGC GCCGCAGCAG CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC 
    CCTCCCTCTG CAGCGGTCCA GCCCACCGCA ACACTAGCGG GTACGAACCC CGAGGAGCCG 
    CCTGGCACCC CTCAGGCCGA CTCCCTTAAC AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC 
    GAACCTGAGC TCGCTCAGCC CCCAACACAC GACCCCCACC AGTCGCTTCC TCCTGGCCCA 
    GATCCAAAGA TGTATAAAAA GGATCTGTAC AGCATGGACG AGCCGGTGCG AGTGACCCAC 
    GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG CAGCAGCCTT TAGGCCCCCC AACCTCGCTC 
    TCCTACAGCC ACCAGCCTGT CTACCAGGAC CAACAGCCAT ACCGCCAGTA CGACCACCCG 
    CCTTATGGCT ATGACGGTGG CGGCTACGCA CAACCAAAGC CTCACAACTA CGACCCGCAC 
    CTGCACTACG ACAACCGTGT GCCTCACTAT AACGAGCAGT GGCCCACGTA CGACCAGCAG 
    CAGCAGCAGC AGACTTCCCC CCAGCCGCCA CCTGCGTCCG GCTTCCCTCA GGGCCACCAG 
    CCTCCGCCTC CACCACCGAT GGGATATGAG CCACGCTCCC CATACGAGGA CGGACCTAAC 
    AGGGATTTCA GCCCTCCTCA GTCCCAGTAT GACGGTGTGT CCCCAATGGG CTACGATCAC 
    CGGCCGCGGC ACCCCAAACC TGCACCGGTC CGATATGAAG AGCCTCCACC TCCACCTCCT 
    CCGGTGTCCT ACGATGCACG GTCACCTTTC GAACCCGAGC CGCATGCTTT CCCAGGCAAC 
    ACACATCGCT CGCCAGACCC ACCGAAACAG TATTATGGAG ACGCCGCTAT GAGGCCCTTG 
    TACAACCCTG GACCTCCGAA CAGAGTGTAC AAACATGAGC CGGTGAACTC TGAGCCTCCT 
    GTACCGCCTC CCAAACCTGA ATCTGTCCTG TCTCCAGGAG AACCGCCCCA TTCTGCAGCT 
    TCCAAACCAT TGCCGCCTCC TCCCAGAGAT GATGACGATG ATGATGACGA TGATGATGAC 
    CCTGCCATGA AGCCCCAGTC TGTGCTCAAC AGGGTCAAAA TGTTCGAAAA CAAACGCTCT 
    GTGTCGGTAG ACCGAGCCAA GGACGCACCT GACGTCACTG GAATTAGGCC CACAGATCTC 
    CCCAAACCTG CGAGCACCCC TGGACCTGTG CTCAAAGCCA ACTCTCTCAG CAACCTGGAG 
    CAGGACAAAC CCTCCTACAG AGCTCCAGAG CCGCAGAAAC CTCAGACGCG TGTTGGGGAC 
    GATGTGGTTC GCTCCAACCA CTACGACCCT GATGATGATG AAGAGTACTA CAGAAAACAG 
    CTCTCATTTT TCGACCGCCG CAGTTTCGAC AGTAAGGTCA TGAATCCACC CAGCGCTGGA 
    GTAAACCGCT TCCATGAGCC GCCCAAACCA CCCCAGACAC AGATCGCTTA TCCTTTCGCT 
    AGGACGGAGT CTGTGGAGAA GGTGAGTCCT GTGGACAAGA GATACGAGCC TCTGCCGCCA 
    GTCAACCCTT CACCTGCTCC ATACAGCCAG AACACACCTG TCGCTCCGCC CACATCCCTG 
    CCCAAACTCA CCAACATCGA GGTGAACTCT CTGCCAGATC CTCACAGCTC TCCCAAAGCC 
    AAACCGGACC TGTCTGCTCT GAGAGCTCCG ACCCGGGACG AGCCCATCCA GAGCAGCTAC 
    TTGCCCCCCA AGTCCACCAT CAATGGCATG GATGCTCCTC CCAAAACACT CGGCGTGCCG 
    ACCAGCTACA ACCGCTACGT CCCCAAGCCT TACACCAGCT CGGCGCGACC CTTCGAGCGC 
    AAGTTCGAGA GTCCGAAATT CAACCACAAC CTGCTTCCCA ACGACACGCA GTCCAAACCC 
    AGTGTCAACA ACAACCTGAA GCCTCAGATC TCCCCACAAC CCCAGGACAC GGACAGCGGC 
    GTCGACACCT TCACTCGCAC CATGGACAAC AGGCCAAAAT ATCAGCACAA CAACATCAAC 
    GCCATTCCCA AAGCCGTCCC AGTCAGCCCC AGTGCACTGG ATGATGATGA AGAAGATGAG 
    GGACACACAG TTGTGGCCAC GGCGCGGGGC ATCTTTAACT GTAATGGCGG GGTGTTGAGC 
    TCTATAGAGA CGGGCGTCAG CATCATTATC CCACAGGGAG CCATTCCTGA TAGTGTGGAG 
    CAGGAGATCT ACTTCAAAGT GTGCCGGGAC AACAGCATCC TGCCTCCTCT GGATAAGGAG 
    AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG ATGTGTGGTC CTCACGGCCT GAAGTTCCTG 
    AAGCCTGTGG AGCTGCGCTT GCCACACTGT GCGTCTATGA CCCCTGATGG TTGGTCTTTT 
    GCTCTAAAAT CCTCCGACTC CTCGTCGGGT GACCCCAAAT GCTGGCAAAA CAAGTGTCTA 
    CCAGGAGACC CCAATTACCT GGTGGGCGCA AACTGTGTGT CTGTGCTGAT CGACCACTTC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326400.1
    CDS110..6232
    Translation

    Target ORF information:

    RefSeq Version XM_021470725.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470725.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GACATCCACT CCGTGGCCTC TGATCATTCC 
    AACCGATCCC ACGACCGGAA GAGGAGCAGC CGCTCTCGTT CTCCAGACCG CCGCTCCGAA 
    CCCTCAGACC ACTCCAGACA CTCTCCACCA CAGATCAGCA ACGGCAGGGC CACAGGGCCG 
    ACGGCTCAGA GAACGCGGCC AGTTCACAGG AGCCGTGATG AAGACAGGAT CTCCAAGGCC 
    GGGCCGCTTC CTCTACCTGC TAAGATGGTG GAGGAGATGC CGAAAGAGCA GAGCGCAGCC 
    AGAGAAACAG AGAAACAGCT CCCCCCTCTG CCAGAGCCCA AACCTGTGTA CGCTCAGCCG 
    GGCCAGCCGG ACGTGGACCT GCCTGTTAGT CCGGCAGATG CTCCTGTGCC CAGCGTCACA 
    CATGATGACA GCATCTTACG GCCGAGCATG AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC 
    GTGGGCTTGA GGCTCGCTGG GGGGAACGAC GTTGGGATAT TTGTGGCGGG AGTTCTGGAG 
    GACAGTCCTG CTGCTAAAGA GGGCCTGGAA GAGGGAGACC AGATTCTCAG GGTAAATAAT 
    GTGGATTTTG CAAATATCAT CCGAGAGGAG GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA 
    GGAGAGGAGG TCACCATCCT GGCCCAGAAG AAGAAAGACG TGTACCGCCG CATTGTAGAG 
    TCTGACGTGG GAGACTCGTT CTACATCAGG ACTCATTTTG AATATGAGAA GGAGTCGCCC 
    TATGGCCTGA GCTTCAATAA GGGGGAGGTC TTCAGAGTCG TCGACACCCT TTACAATGGC 
    AAACTGGGCT CCTGGCTCGC CATCCGCATC GGCAAAAACC ACCAGGAGGT GGAGCGGGGC 
    ATCATCCCCA ACAAGAACAG GGCTGAGCAG TTATCAAGTG TCCAGTACAC TCTCCCAAAG 
    ACTCCAGGAG GGGATCGCGC CGACTTCTGG AGATTCAGAG GCCTCCGCAG CTCCAAACGA 
    AACCTGAGGA AGAGCAGAGA AGACCTGTCT GCTCAGCCGG TCCAAACCAA ATTCCCTGCT 
    TATGAAAGAG TGGTGCTTAG AGAAGCTGGT TTCTTGAGGC CAGTGGTCAT TTTCGGACCG 
    ATCGCGGATG TGGCTAGAGA GAAGCTGTCC AGAGAGGAGA CTGACCTCTT CGAACTTGCA 
    AAGAGTGAAC CCAGAGATGC GGGCACAGAC CAGCGCAGCT CCGGAATCAT CCGTCTCCAT 
    ACCATTAAAC AGATCATTGA CCGAGACAAG CACGCGGTGC TGGACATCAC CCCTAATGCG 
    GTGGACCGTC TGAACTACGC TCAGTGGTAC CCCATCGTGG TGTTTCTGAA TCCAGACAGC 
    AAACAGGGCG TCAAGAACAT GAGGACCAGG CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA 
    AAGCTCTACG AGCGTGCTCT CAAACTGAGG AAGAACAACC ATCACCTTTT CACCACCACT 
    ATTAACTTGA ACAACATGAA TGACGGGTGG TACGGTGCTT TGAAAGAGAG CATCCAGCAG 
    CAGCAGAACC AGTTGGTCTG GGTCTCAGAG GGCAAGGCCG ACGGCGCTCC TGACGATGAC 
    CTTGACATCC ATGACGACCG CCTCTCCTAC CTCTCGGCTC CGGGCAGCGA GTACAGCATG 
    TACAGCACCG ACAGCCGCCA CACGTCCGAC TATGAGGACA CCGACACGGA GGGTGGTGCC 
    TACACCGACC AGGAGCTGGA CGAGACCATG AACGATGAGG TGGGCCTGCC TAGCGAGCCT 
    GCCATCACAC GCTCATCTGA GCCTGTGCGA GAGGACCCGC CAGTCATTCA GGACGCAGCT 
    GGATACCCCA GCTACCAGCA TGCGGTACCG CAACCTGAAG CGGTGAGCCG CATTGACCCG 
    GCCGGGTTTA AGATGGCAGC GCCGCAGCAG CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC 
    CCTCCCTCTG CAGCGGTCCA GCCCACCGCA ACACTAGCGG GTACGAACCC CGAGGAGCCG 
    CCTGGCACCC CTCAGGCCGA CTCCCTTAAC AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC 
    GAACCTGAGC TCGCTCAGCC CCCAACACAC GACCCCCACC AGTCGCTTCC TCCTGGCCCA 
    GATCCAAAGA TGTATAAAAA GGATCTGTAC AGCATGGACG AGCCGGTGCG AGTGACCCAC 
    GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG CAGCAGCCTT TAGGCCCCCC AACCTCGCTC 
    TCCTACAGCC ACCAGCCTGT CTACCAGGAC CAACAGCCAT ACCGCCAGTA CGACCACCCG 
    CCTTATGGCT ATGACGGTGG CGGCTACGCA CAACCAAAGC CTCACAACTA CGACCCGCAC 
    CTGCACTACG ACAACCGTGT GCCTCACTAT AACGAGCAGT GGCCCACGTA CGACCAGCAG 
    CAGCAGCAGC AGACTTCCCC CCAGCCGCCA CCTGCGTCCG GCTTCCCTCA GGGCCACCAG 
    CCTCCGCCTC CACCACCGAT GGGATATGAG CCACGCTCCC CATACGAGGA CGGACCTAAC 
    AGGGATTTCA GCCCTCCTCA GTCCCAGTAT GACGGTGTGT CCCCAATGGG CTACGATCAC 
    CGGCCGCGGC ACCCCAAACC TGCACCGGTC CGATATGAAG AGCCTCCACC TCCACCTCCT 
    CCGGTGTCCT ACGATGCACG GTCACCTTTC GAACCCGAGC CGCATGCTTT CCCAGGCAAC 
    ACACATCGCT CGCCAGACCC ACCGAAACAG TATTATGGAG ACGCCGCTAT GAGGCCCTTG 
    TACAACCCTG GACCTCCGAA CAGAGTGTAC AAACATGAGC CGGTGAACTC TGAGCCTCCT 
    GTACCGCCTC CCAAACCTGA ATCTGTCCTG TCTCCAGGAG AACCGCCCCA TTCTGCAGCT 
    TCCAAACCAT TGCCGCCTCC TCCCAGAGAT GATGACGATG ATGATGACGA TGATGATGAC 
    CCTGCCATGA AGCCCCAGTC TGTGCTCAAC AGGGTCAAAA TGTTCGAAAA CAAACGCTCT 
    GTGTCGGTAG ACCGAGCCAA GGACGCACCT GACGTCACTG GAATTAGGCC CACAGATCTC 
    CCCAAACCTG CGAGCACCCC TGGACCTGTG CTCAAAGCCA ACTCTCTCAG CAACCTGGAG 
    CAGGACAAAC CCTCCTACAG AGCTCCAGAG CCGCAGAAAC CTCAGACGCG TGTTGGGGAC 
    GATGTGGTTC GCTCCAACCA CTACGACCCT GATGATGATG AAGAGTACTA CAGAAAACAG 
    CTCTCATTTT TCGACCGCCG CAGTTTCGAC AGTAAGGTCA TGAATCCACC CAGCGCTGGA 
    GTAAACCGCT TCCATGAGCC GCCCAAACCA CCCCAGACAC AGATCGCTTA TCCTTTCGCT 
    AGGACGGAGT CTGTGGAGAA GGTGAGTCCT GTGGACAAGA GATACGAGCC TCTGCCGCCA 
    GTCAACCCTT CACCTGCTCC ATACAGCCAG AACACACCTG TCGCTCCGCC CACATCCCTG 
    CCCAAACTCA CCAACATCGA GGTGAACTCT CTGCCAGATC CTCACAGCTC TCCCAAAGCC 
    AAACCGGACC TGTCTGCTCT GAGAGCTCCG ACCCGGGACG AGCCCATCCA GAGCAGCTAC 
    TTGCCCCCCA AGTCCACCAT CAATGGCATG GATGCTCCTC CCAAAACACT CGGCGTGCCG 
    ACCAGCTACA ACCGCTACGT CCCCAAGCCT TACACCAGCT CGGCGCGACC CTTCGAGCGC 
    AAGTTCGAGA GTCCGAAATT CAACCACAAC CTGCTTCCCA ACGACACGCA GTCCAAACCC 
    AGTGTCAACA ACAACCTGAA GCCTCAGATC TCCCCACAAC CCCAGGACAC GGACAGCGGC 
    GTCGACACCT TCACTCGCAC CATGGACAAC AGGCCAAAAT ATCAGCACAA CAACATCAAC 
    GCCATTCCCA AAGCCGTCCC AGTCAGCCCC AGTGCACTGG ATGATGATGA AGAAGATGAG 
    GGACACACAG TTGTGGCCAC GGCGCGGGGC ATCTTTAACT GTAATGGCGG GGTGTTGAGC 
    TCTATAGAGA CGGGCGTCAG CATCATTATC CCACAGGGAG CCATTCCTGA TAGTGTGGAG 
    CAGGAGATCT ACTTCAAAGT GTGCCGGGAC AACAGCATCC TGCCTCCTCT GGATAAGGAG 
    AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG ATGTGTGGTC CTCACGGCCT GAAGTTCCTG 
    AAGCCTGTGG AGCTGCGCTT GCCACACTGT GCGTCTATGA CCCCTGATGG TTGGTCTTTT 
    GCTCTAAAAT CCTCCGACTC CTCGTCGGGT GACCCCAAAT GCTGGCAAAA CAAGTGTCTA 
    CCAGGAGACC CCAATTACCT GGTGGGCGCA AACTGTGTGT CTGTGCTGAT CGACCACTTC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470726.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326401.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa44618
    Clone ID Related Accession (Same CDS sequence) XM_021470726.1
    Accession Version XM_021470726.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
    ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
    TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
    AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
    GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
    AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
    GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
    GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
    GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
    CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
    ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
    GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
    TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
    GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
    CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
    TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
    TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
    TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
    GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
    CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
    CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
    CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
    AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
    ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
    ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
    GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
    TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
    GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
    AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
    GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
    ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
    TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
    GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
    CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
    CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
    GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
    ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
    GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
    GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
    GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
    GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
    GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
    TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
    CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
    CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
    AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
    GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
    TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
    GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
    AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
    ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
    AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
    GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
    AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
    AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
    CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
    CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
    ACATCCCTGC CCAAACTCAC CAACATCGAG GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326401.1
    CDS110..6226
    Translation

    Target ORF information:

    RefSeq Version XM_021470726.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
    ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
    TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
    AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
    GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
    AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
    GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
    GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
    GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
    CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
    ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
    GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
    TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
    GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
    CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
    TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
    TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
    TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
    GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
    CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
    CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
    CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
    AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
    ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
    ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
    GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
    TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
    GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
    AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
    GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
    ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
    TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
    GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
    CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
    CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
    GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
    ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
    GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
    GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
    GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
    GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
    GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
    TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
    CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
    CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
    AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
    GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
    TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
    GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
    AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
    ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
    AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
    GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
    AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
    AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
    CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
    CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
    ACATCCCTGC CCAAACTCAC CAACATCGAG GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470727.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326402.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa44619
    Clone ID Related Accession (Same CDS sequence) XM_021470727.1
    Accession Version XM_021470727.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGTCAC 
    AGGAGCCGTG ATGAAGACAG GATCTCCAAG GCCGGGCCGC TTCCTCTACC TGCTAAGATG 
    GTGGAGGAGA TGCCGAAAGA GCAGAGCGCA GCCAGAGAAA CAGAGAAACA GCTCCCCCCT 
    CTGCCAGAGC CCAAACCTGT GTACGCTCAG CCGGGCCAGC CGGACGTGGA CCTGCCTGTT 
    AGTCCGGCAG ATGCTCCTGT GCCCAGCGTC ACACATGATG ACAGCATCTT ACGGCCGAGC 
    ATGAAGCTGG TGAAGTTCAA GAAGGGCGAG AGCGTGGGCT TGAGGCTCGC TGGGGGGAAC 
    GACGTTGGGA TATTTGTGGC GGGAGTTCTG GAGGACAGTC CTGCTGCTAA AGAGGGCCTG 
    GAAGAGGGAG ACCAGATTCT CAGGGTAAAT AATGTGGATT TTGCAAATAT CATCCGAGAG 
    GAGGCAGTGC TGTTCCTGCT GGATCTGCCC AAAGGAGAGG AGGTCACCAT CCTGGCCCAG 
    AAGAAGAAAG ACGTGTACCG CCGCATTGTA GAGTCTGACG TGGGAGACTC GTTCTACATC 
    AGGACTCATT TTGAATATGA GAAGGAGTCG CCCTATGGCC TGAGCTTCAA TAAGGGGGAG 
    GTCTTCAGAG TCGTCGACAC CCTTTACAAT GGCAAACTGG GCTCCTGGCT CGCCATCCGC 
    ATCGGCAAAA ACCACCAGGA GGTGGAGCGG GGCATCATCC CCAACAAGAA CAGGGCTGAG 
    CAGTTATCAA GTGTCCAGTA CACTCTCCCA AAGACTCCAG GAGGGGATCG CGCCGACTTC 
    TGGAGATTCA GAGGCCTCCG CAGCTCCAAA CGAAACCTGA GGAAGAGCAG AGAAGACCTG 
    TCTGCTCAGC CGGTCCAAAC CAAATTCCCT GCTTATGAAA GAGTGGTGCT TAGAGAAGCT 
    GGTTTCTTGA GGCCAGTGGT CATTTTCGGA CCGATCGCGG ATGTGGCTAG AGAGAAGCTG 
    TCCAGAGAGG AGACTGACCT CTTCGAACTT GCAAAGAGTG AACCCAGAGA TGCGGGCACA 
    GACCAGCGCA GCTCCGGAAT CATCCGTCTC CATACCATTA AACAGATCAT TGACCGAGAC 
    AAGCACGCGG TGCTGGACAT CACCCCTAAT GCGGTGGACC GTCTGAACTA CGCTCAGTGG 
    TACCCCATCG TGGTGTTTCT GAATCCAGAC AGCAAACAGG GCGTCAAGAA CATGAGGACC 
    AGGCTCTGTC CCGAGTCCAG GAAGAGCGCC CGAAAGCTCT ACGAGCGTGC TCTCAAACTG 
    AGGAAGAACA ACCATCACCT TTTCACCACC ACTATTAACT TGAACAACAT GAATGACGGG 
    TGGTACGGTG CTTTGAAAGA GAGCATCCAG CAGCAGCAGA ACCAGTTGGT CTGGGTCTCA 
    GAGGGCAAGG CCGACGGCGC TCCTGACGAT GACCTTGACA TCCATGACGA CCGCCTCTCC 
    TACCTCTCGG CTCCGGGCAG CGAGTACAGC ATGTACAGCA CCGACAGCCG CCACACGTCC 
    GACTATGAGG ACACCGACAC GGAGGGTGGT GCCTACACCG ACCAGGAGCT GGACGAGACC 
    ATGAACGATG AGGTGGGCCT GCCTAGCGAG CCTGCCATCA CACGCTCATC TGAGCCTGTG 
    CGAGAGGACC CGCCAGTCAT TCAGGACGCA GCTGGATACC CCAGCTACCA GCATGCGGTA 
    CCGCAACCTG AAGCGGTGAG CCGCATTGAC CCGGCCGGGT TTAAGATGGC AGCGCCGCAG 
    CAGCAAGCTG AGGCTGCTCT GGCCTCGCCG CCCCCTCCCT CTGCAGCGGT CCAGCCCACC 
    GCAACACTAG CGGGTACGAA CCCCGAGGAG CCGCCTGGCA CCCCTCAGGC CGACTCCCTT 
    AACAGCCCCG TCCCTGTTCC CGACCCCCAG CCCGAACCTG AGCTCGCTCA GCCCCCAACA 
    CACGACCCCC ACCAGTCGCT TCCTCCTGGC CCAGATCCAA AGATGTATAA AAAGGATCTG 
    TACAGCATGG ACGAGCCGGT GCGAGTGACC CACGGCGTGA AGCCTCCCCC TCCCCAGCAG 
    CAGCAGCAGC CTTTAGGCCC CCCAACCTCG CTCTCCTACA GCCACCAGCC TGTCTACCAG 
    GACCAACAGC CATACCGCCA GTACGACCAC CCGCCTTATG GCTATGACGG TGGCGGCTAC 
    GCACAACCAA AGCCTCACAA CTACGACCCG CACCTGCACT ACGACAACCG TGTGCCTCAC 
    TATAACGAGC AGTGGCCCAC GTACGACCAG CAGCAGCAGC AGCAGACTTC CCCCCAGCCG 
    CCACCTGCGT CCGGCTTCCC TCAGGGCCAC CAGCCTCCGC CTCCACCACC GATGGGATAT 
    GAGCCACGCT CCCCATACGA GGACGGACCT AACAGGGATT TCAGCCCTCC TCAGTCCCAG 
    TATGACGGTG TGTCCCCAAT GGGCTACGAT CACCGGCCGC GGCACCCCAA ACCTGCACCG 
    GTCCGATATG AAGAGCCTCC ACCTCCACCT CCTCCGGTGT CCTACGATGC ACGGTCACCT 
    TTCGAACCCG AGCCGCATGC TTTCCCAGGC AACACACATC GCTCGCCAGA CCCACCGAAA 
    CAGTATTATG GAGACGCCGC TATGAGGCCC TTGTACAACC CTGGACCTCC GAACAGAGTG 
    TACAAACATG AGCCGGTGAA CTCTGAGCCT CCTGTACCGC CTCCCAAACC TGAATCTGTC 
    CTGTCTCCAG GAGAACCGCC CCATTCTGCA GCTTCCAAAC CATTGCCGCC TCCTCCCAGA 
    GATGATGACG ATGATGATGA CGATGATGAT GACCCTGCCA TGAAGCCCCA GTCTGTGCTC 
    AACAGGGTCA AAATGTTCGA AAACAAACGC TCTGTGTCGG TAGACCGAGC CAAGGACGCA 
    CCTGACGTCA CTGGAATTAG GCCCACAGAT CTCCCCAAAC CTGCGAGCAC CCCTGGACCT 
    GTGCTCAAAG CCAACTCTCT CAGCAACCTG GAGCAGGACA AACCCTCCTA CAGAGCTCCA 
    GAGCCGCAGA AACCTCAGAC GCGTGTTGGG GACGATGTGG TTCGCTCCAA CCACTACGAC 
    CCTGATGATG ATGAAGAGTA CTACAGAAAA CAGCTCTCAT TTTTCGACCG CCGCAGTTTC 
    GACAGTAAGG TCATGAATCC ACCCAGCGCT GGAGTAAACC GCTTCCATGA GCCGCCCAAA 
    CCACCCCAGA CACAGATCGC TTATCCTTTC GCTAGGACGG AGTCTGTGGA GAAGGTGAGT 
    CCTGTGGACA AGAGATACGA GCCTCTGCCG CCAGTCAACC CTTCACCTGC TCCATACAGC 
    CAGAACACAC CTGTCGCTCC GCCCACATCC CTGCCCAAAC TCACCAACAT CGAGGTGAAC 
    TCTCTGCCAG ATCCTCACAG CTCTCCCAAA GCCAAACCGG ACCTGTCTGC TCTGAGAGCT 
    CCGACCCGGG ACGAGCCCAT CCAGAGCAGC TACTTGCCCC CCAAGTCCAC CATCAATGGC 
    ATGGATGCTC CTCCCAAAAC ACTCGGCGTG CCGACCAGCT ACAACCGCTA CGTCCCCAAG 
    CCTTACACCA GCTCGGCGCG ACCCTTCGAG CGCAAGTTCG AGAGTCCGAA ATTCAACCAC 
    AACCTGCTTC CCAACGACAC GCAGTCCAAA CCCAGTGTCA ACAACAACCT GAAGCCTCAG 
    ATCTCCCCAC AACCCCAGGA CACGGACAGC GGCGTCGACA CCTTCACTCG CACCATGGAC 
    AACAGGCCAA AATATCAGCA CAACAACATC AACGCCATTC CCAAAGCCGT CCCAGTCAGC 
    CCCAGTGCAC TGGATGATGA TGAAGAAGAT GAGGGACACA CAGTTGTGGC CACGGCGCGG 
    GGCATCTTTA ACTGTAATGG CGGGGTGTTG AGCTCTATAG AGACGGGCGT CAGCATCATT 
    ATCCCACAGG GAGCCATTCC TGATAGTGTG GAGCAGGAGA TCTACTTCAA AGTGTGCCGG 
    GACAACAGCA TCCTGCCTCC TCTGGATAAG GAGAAAGGTG AAACTCTGCT GAGTCCGCTG 
    GTGATGTGTG GTCCTCACGG CCTGAAGTTC CTGAAGCCTG TGGAGCTGCG CTTGCCACAC 
    TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCCGA CTCCTCGTCG 
    GGTGACCCCA AATGCTGGCA AAACAAGTGT CTACCAGGAG ACCCCAATTA CCTGGTGGGC 
    GCAAACTGTG TGTCTGTGCT GATCGACCAC TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326402.1
    CDS110..6205
    Translation

    Target ORF information:

    RefSeq Version XM_021470727.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGTCAC 
    AGGAGCCGTG ATGAAGACAG GATCTCCAAG GCCGGGCCGC TTCCTCTACC TGCTAAGATG 
    GTGGAGGAGA TGCCGAAAGA GCAGAGCGCA GCCAGAGAAA CAGAGAAACA GCTCCCCCCT 
    CTGCCAGAGC CCAAACCTGT GTACGCTCAG CCGGGCCAGC CGGACGTGGA CCTGCCTGTT 
    AGTCCGGCAG ATGCTCCTGT GCCCAGCGTC ACACATGATG ACAGCATCTT ACGGCCGAGC 
    ATGAAGCTGG TGAAGTTCAA GAAGGGCGAG AGCGTGGGCT TGAGGCTCGC TGGGGGGAAC 
    GACGTTGGGA TATTTGTGGC GGGAGTTCTG GAGGACAGTC CTGCTGCTAA AGAGGGCCTG 
    GAAGAGGGAG ACCAGATTCT CAGGGTAAAT AATGTGGATT TTGCAAATAT CATCCGAGAG 
    GAGGCAGTGC TGTTCCTGCT GGATCTGCCC AAAGGAGAGG AGGTCACCAT CCTGGCCCAG 
    AAGAAGAAAG ACGTGTACCG CCGCATTGTA GAGTCTGACG TGGGAGACTC GTTCTACATC 
    AGGACTCATT TTGAATATGA GAAGGAGTCG CCCTATGGCC TGAGCTTCAA TAAGGGGGAG 
    GTCTTCAGAG TCGTCGACAC CCTTTACAAT GGCAAACTGG GCTCCTGGCT CGCCATCCGC 
    ATCGGCAAAA ACCACCAGGA GGTGGAGCGG GGCATCATCC CCAACAAGAA CAGGGCTGAG 
    CAGTTATCAA GTGTCCAGTA CACTCTCCCA AAGACTCCAG GAGGGGATCG CGCCGACTTC 
    TGGAGATTCA GAGGCCTCCG CAGCTCCAAA CGAAACCTGA GGAAGAGCAG AGAAGACCTG 
    TCTGCTCAGC CGGTCCAAAC CAAATTCCCT GCTTATGAAA GAGTGGTGCT TAGAGAAGCT 
    GGTTTCTTGA GGCCAGTGGT CATTTTCGGA CCGATCGCGG ATGTGGCTAG AGAGAAGCTG 
    TCCAGAGAGG AGACTGACCT CTTCGAACTT GCAAAGAGTG AACCCAGAGA TGCGGGCACA 
    GACCAGCGCA GCTCCGGAAT CATCCGTCTC CATACCATTA AACAGATCAT TGACCGAGAC 
    AAGCACGCGG TGCTGGACAT CACCCCTAAT GCGGTGGACC GTCTGAACTA CGCTCAGTGG 
    TACCCCATCG TGGTGTTTCT GAATCCAGAC AGCAAACAGG GCGTCAAGAA CATGAGGACC 
    AGGCTCTGTC CCGAGTCCAG GAAGAGCGCC CGAAAGCTCT ACGAGCGTGC TCTCAAACTG 
    AGGAAGAACA ACCATCACCT TTTCACCACC ACTATTAACT TGAACAACAT GAATGACGGG 
    TGGTACGGTG CTTTGAAAGA GAGCATCCAG CAGCAGCAGA ACCAGTTGGT CTGGGTCTCA 
    GAGGGCAAGG CCGACGGCGC TCCTGACGAT GACCTTGACA TCCATGACGA CCGCCTCTCC 
    TACCTCTCGG CTCCGGGCAG CGAGTACAGC ATGTACAGCA CCGACAGCCG CCACACGTCC 
    GACTATGAGG ACACCGACAC GGAGGGTGGT GCCTACACCG ACCAGGAGCT GGACGAGACC 
    ATGAACGATG AGGTGGGCCT GCCTAGCGAG CCTGCCATCA CACGCTCATC TGAGCCTGTG 
    CGAGAGGACC CGCCAGTCAT TCAGGACGCA GCTGGATACC CCAGCTACCA GCATGCGGTA 
    CCGCAACCTG AAGCGGTGAG CCGCATTGAC CCGGCCGGGT TTAAGATGGC AGCGCCGCAG 
    CAGCAAGCTG AGGCTGCTCT GGCCTCGCCG CCCCCTCCCT CTGCAGCGGT CCAGCCCACC 
    GCAACACTAG CGGGTACGAA CCCCGAGGAG CCGCCTGGCA CCCCTCAGGC CGACTCCCTT 
    AACAGCCCCG TCCCTGTTCC CGACCCCCAG CCCGAACCTG AGCTCGCTCA GCCCCCAACA 
    CACGACCCCC ACCAGTCGCT TCCTCCTGGC CCAGATCCAA AGATGTATAA AAAGGATCTG 
    TACAGCATGG ACGAGCCGGT GCGAGTGACC CACGGCGTGA AGCCTCCCCC TCCCCAGCAG 
    CAGCAGCAGC CTTTAGGCCC CCCAACCTCG CTCTCCTACA GCCACCAGCC TGTCTACCAG 
    GACCAACAGC CATACCGCCA GTACGACCAC CCGCCTTATG GCTATGACGG TGGCGGCTAC 
    GCACAACCAA AGCCTCACAA CTACGACCCG CACCTGCACT ACGACAACCG TGTGCCTCAC 
    TATAACGAGC AGTGGCCCAC GTACGACCAG CAGCAGCAGC AGCAGACTTC CCCCCAGCCG 
    CCACCTGCGT CCGGCTTCCC TCAGGGCCAC CAGCCTCCGC CTCCACCACC GATGGGATAT 
    GAGCCACGCT CCCCATACGA GGACGGACCT AACAGGGATT TCAGCCCTCC TCAGTCCCAG 
    TATGACGGTG TGTCCCCAAT GGGCTACGAT CACCGGCCGC GGCACCCCAA ACCTGCACCG 
    GTCCGATATG AAGAGCCTCC ACCTCCACCT CCTCCGGTGT CCTACGATGC ACGGTCACCT 
    TTCGAACCCG AGCCGCATGC TTTCCCAGGC AACACACATC GCTCGCCAGA CCCACCGAAA 
    CAGTATTATG GAGACGCCGC TATGAGGCCC TTGTACAACC CTGGACCTCC GAACAGAGTG 
    TACAAACATG AGCCGGTGAA CTCTGAGCCT CCTGTACCGC CTCCCAAACC TGAATCTGTC 
    CTGTCTCCAG GAGAACCGCC CCATTCTGCA GCTTCCAAAC CATTGCCGCC TCCTCCCAGA 
    GATGATGACG ATGATGATGA CGATGATGAT GACCCTGCCA TGAAGCCCCA GTCTGTGCTC 
    AACAGGGTCA AAATGTTCGA AAACAAACGC TCTGTGTCGG TAGACCGAGC CAAGGACGCA 
    CCTGACGTCA CTGGAATTAG GCCCACAGAT CTCCCCAAAC CTGCGAGCAC CCCTGGACCT 
    GTGCTCAAAG CCAACTCTCT CAGCAACCTG GAGCAGGACA AACCCTCCTA CAGAGCTCCA 
    GAGCCGCAGA AACCTCAGAC GCGTGTTGGG GACGATGTGG TTCGCTCCAA CCACTACGAC 
    CCTGATGATG ATGAAGAGTA CTACAGAAAA CAGCTCTCAT TTTTCGACCG CCGCAGTTTC 
    GACAGTAAGG TCATGAATCC ACCCAGCGCT GGAGTAAACC GCTTCCATGA GCCGCCCAAA 
    CCACCCCAGA CACAGATCGC TTATCCTTTC GCTAGGACGG AGTCTGTGGA GAAGGTGAGT 
    CCTGTGGACA AGAGATACGA GCCTCTGCCG CCAGTCAACC CTTCACCTGC TCCATACAGC 
    CAGAACACAC CTGTCGCTCC GCCCACATCC CTGCCCAAAC TCACCAACAT CGAGGTGAAC 
    TCTCTGCCAG ATCCTCACAG CTCTCCCAAA GCCAAACCGG ACCTGTCTGC TCTGAGAGCT 
    CCGACCCGGG ACGAGCCCAT CCAGAGCAGC TACTTGCCCC CCAAGTCCAC CATCAATGGC 
    ATGGATGCTC CTCCCAAAAC ACTCGGCGTG CCGACCAGCT ACAACCGCTA CGTCCCCAAG 
    CCTTACACCA GCTCGGCGCG ACCCTTCGAG CGCAAGTTCG AGAGTCCGAA ATTCAACCAC 
    AACCTGCTTC CCAACGACAC GCAGTCCAAA CCCAGTGTCA ACAACAACCT GAAGCCTCAG 
    ATCTCCCCAC AACCCCAGGA CACGGACAGC GGCGTCGACA CCTTCACTCG CACCATGGAC 
    AACAGGCCAA AATATCAGCA CAACAACATC AACGCCATTC CCAAAGCCGT CCCAGTCAGC 
    CCCAGTGCAC TGGATGATGA TGAAGAAGAT GAGGGACACA CAGTTGTGGC CACGGCGCGG 
    GGCATCTTTA ACTGTAATGG CGGGGTGTTG AGCTCTATAG AGACGGGCGT CAGCATCATT 
    ATCCCACAGG GAGCCATTCC TGATAGTGTG GAGCAGGAGA TCTACTTCAA AGTGTGCCGG 
    GACAACAGCA TCCTGCCTCC TCTGGATAAG GAGAAAGGTG AAACTCTGCT GAGTCCGCTG 
    GTGATGTGTG GTCCTCACGG CCTGAAGTTC CTGAAGCCTG TGGAGCTGCG CTTGCCACAC 
    TGTGCGTCTA TGACCCCTGA TGGTTGGTCT TTTGCTCTAA AATCCTCCGA CTCCTCGTCG 
    GGTGACCCCA AATGCTGGCA AAACAAGTGT CTACCAGGAG ACCCCAATTA CCTGGTGGGC 
    GCAAACTGTG TGTCTGTGCT GATCGACCAC TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_009298130.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296405.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa44620
    Clone ID Related Accession (Same CDS sequence) XM_009298130.2 , XM_009298130.3
    Accession Version XM_009298130.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
    ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
    AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
    GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
    CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
    ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
    GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
    TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
    CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
    TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
    AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
    AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
    ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
    CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
    AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
    ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
    GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
    GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
    AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296405.1
    CDS115..6207
    Translation

    Target ORF information:

    RefSeq Version XM_009298130.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298130.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
    ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
    AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
    GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
    CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
    ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
    GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
    TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
    CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
    TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
    AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
    AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
    ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
    CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
    AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
    ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
    GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
    GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
    AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_009298130.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296405.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa44620
    Clone ID Related Accession (Same CDS sequence) XM_009298130.2 , XM_009298130.3
    Accession Version XM_009298130.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298130.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
    ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
    AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
    GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
    CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
    ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
    GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
    TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
    CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
    TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
    AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
    AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
    ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
    CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
    AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
    ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
    GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
    GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
    AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296405.1
    CDS110..6202
    Translation

    Target ORF information:

    RefSeq Version XM_009298130.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298130.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
    ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
    AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
    GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
    CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
    ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
    GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
    TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
    CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
    TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
    AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
    AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
    ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
    CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
    AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
    ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
    GCGTCTATGA CCCCTGATGG TTGGTCTTTT GCTCTAAAAT CCTCCGACTC CTCGTCGGGT 
    GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
    AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470728.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326403.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID ODa44621
    Clone ID Related Accession (Same CDS sequence) XM_021470728.1
    Accession Version XM_021470728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
    ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
    TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
    AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
    GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
    AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
    GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
    GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
    GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
    CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
    ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
    GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
    TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
    GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
    CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
    TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
    TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
    TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
    GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
    CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
    CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
    CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
    AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
    ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
    ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
    GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
    TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
    GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
    AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
    GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
    ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
    TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
    GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
    CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
    CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
    GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
    ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
    GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
    GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
    GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
    GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
    GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
    TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
    CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
    CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
    AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
    GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
    TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
    GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
    AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
    ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
    AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
    GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
    AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
    AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
    CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
    CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
    ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
    CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
    AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
    GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
    TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
    TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
    GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
    AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
    GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
    GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
    AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
    GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
    AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG CGTCTATGAC CCCTGATGGT 
    GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
    AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326403.1
    CDS110..6202
    Translation

    Target ORF information:

    RefSeq Version XM_021470728.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
    ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
    TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
    AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
    GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
    AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
    GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
    GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
    GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
    CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
    ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
    GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
    TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
    GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
    CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
    TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
    TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
    TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
    GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
    CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
    CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
    CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
    AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
    ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
    ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
    GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
    TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
    GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
    AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
    GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
    ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
    TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
    GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
    CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
    CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
    GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
    ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
    GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
    GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
    GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
    GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
    GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
    TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
    CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
    CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
    AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
    GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
    TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
    GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
    AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
    ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
    AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
    GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
    AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
    AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
    CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
    CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
    ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
    CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
    AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
    GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
    TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
    TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
    GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
    AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
    GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
    GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
    AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
    GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
    AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG CGTCTATGAC CCCTGATGGT 
    GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
    AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_009298132.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296407.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa44622
    Clone ID Related Accession (Same CDS sequence) XM_009298132.2 , XM_009298132.3
    Accession Version XM_009298132.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298132.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGGGCCAC AGGGCCGACG GCTCAGAGAA CGCGGCCAGT TCACAGGAGC 
    CGTGATGAAG ACAGGATCTC CAAGGCCGGG CCGCTTCCTC TACCTGCTAA GATGGTGGAG 
    GAGATGCCGA AAGAGCAGAG CGCAGCCAGA GAAACAGAGA AACAGCTCCC CCCTCTGCCA 
    GAGCCCAAAC CTGTGTACGC TCAGCCGGGC CAGCCGGACG TGGACCTGCC TGTTAGTCCG 
    GCAGATGCTC CTGTGCCCAG CGTCACACAT GATGACAGCA TCTTACGGCC GAGCATGAAG 
    CTGGTGAAGT TCAAGAAGGG CGAGAGCGTG GGCTTGAGGC TCGCTGGGGG GAACGACGTT 
    GGGATATTTG TGGCGGGAGT TCTGGAGGAC AGTCCTGCTG CTAAAGAGGG CCTGGAAGAG 
    GGAGACCAGA TTCTCAGGGT AAATAATGTG GATTTTGCAA ATATCATCCG AGAGGAGGCA 
    GTGCTGTTCC TGCTGGATCT GCCCAAAGGA GAGGAGGTCA CCATCCTGGC CCAGAAGAAG 
    AAAGACGTGT ACCGCCGCAT TGTAGAGTCT GACGTGGGAG ACTCGTTCTA CATCAGGACT 
    CATTTTGAAT ATGAGAAGGA GTCGCCCTAT GGCCTGAGCT TCAATAAGGG GGAGGTCTTC 
    AGAGTCGTCG ACACCCTTTA CAATGGCAAA CTGGGCTCCT GGCTCGCCAT CCGCATCGGC 
    AAAAACCACC AGGAGGTGGA GCGGGGCATC ATCCCCAACA AGAACAGGGC TGAGCAGTTA 
    TCAAGTGTCC AGTACACTCT CCCAAAGACT CCAGGAGGGG ATCGCGCCGA CTTCTGGAGA 
    TTCAGAGGCC TCCGCAGCTC CAAACGAAAC CTGAGGAAGA GCAGAGAAGA CCTGTCTGCT 
    CAGCCGGTCC AAACCAAATT CCCTGCTTAT GAAAGAGTGG TGCTTAGAGA AGCTGGTTTC 
    TTGAGGCCAG TGGTCATTTT CGGACCGATC GCGGATGTGG CTAGAGAGAA GCTGTCCAGA 
    GAGGAGACTG ACCTCTTCGA ACTTGCAAAG AGTGAACCCA GAGATGCGGG CACAGACCAG 
    CGCAGCTCCG GAATCATCCG TCTCCATACC ATTAAACAGA TCATTGACCG AGACAAGCAC 
    GCGGTGCTGG ACATCACCCC TAATGCGGTG GACCGTCTGA ACTACGCTCA GTGGTACCCC 
    ATCGTGGTGT TTCTGAATCC AGACAGCAAA CAGGGCGTCA AGAACATGAG GACCAGGCTC 
    TGTCCCGAGT CCAGGAAGAG CGCCCGAAAG CTCTACGAGC GTGCTCTCAA ACTGAGGAAG 
    AACAACCATC ACCTTTTCAC CACCACTATT AACTTGAACA ACATGAATGA CGGGTGGTAC 
    GGTGCTTTGA AAGAGAGCAT CCAGCAGCAG CAGAACCAGT TGGTCTGGGT CTCAGAGGGC 
    AAGGCCGACG GCGCTCCTGA CGATGACCTT GACATCCATG ACGACCGCCT CTCCTACCTC 
    TCGGCTCCGG GCAGCGAGTA CAGCATGTAC AGCACCGACA GCCGCCACAC GTCCGACTAT 
    GAGGACACCG ACACGGAGGG TGGTGCCTAC ACCGACCAGG AGCTGGACGA GACCATGAAC 
    GATGAGGTGG GCCTGCCTAG CGAGCCTGCC ATCACACGCT CATCTGAGCC TGTGCGAGAG 
    GACCCGCCAG TCATTCAGGA CGCAGCTGGA TACCCCAGCT ACCAGCATGC GGTACCGCAA 
    CCTGAAGCGG TGAGCCGCAT TGACCCGGCC GGGTTTAAGA TGGCAGCGCC GCAGCAGCAA 
    GCTGAGGCTG CTCTGGCCTC GCCGCCCCCT CCCTCTGCAG CGGTCCAGCC CACCGCAACA 
    CTAGCGGGTA CGAACCCCGA GGAGCCGCCT GGCACCCCTC AGGCCGACTC CCTTAACAGC 
    CCCGTCCCTG TTCCCGACCC CCAGCCCGAA CCTGAGCTCG CTCAGCCCCC AACACACGAC 
    CCCCACCAGT CGCTTCCTCC TGGCCCAGAT CCAAAGATGT ATAAAAAGGA TCTGTACAGC 
    ATGGACGAGC CGGTGCGAGT GACCCACGGC GTGAAGCCTC CCCCTCCCCA GCAGCAGCAG 
    CAGCCTTTAG GCCCCCCAAC CTCGCTCTCC TACAGCCACC AGCCTGTCTA CCAGGACCAA 
    CAGCCATACC GCCAGTACGA CCACCCGCCT TATGGCTATG ACGGTGGCGG CTACGCACAA 
    CCAAAGCCTC ACAACTACGA CCCGCACCTG CACTACGACA ACCGTGTGCC TCACTATAAC 
    GAGCAGTGGC CCACGTACGA CCAGCAGCAG CAGCAGCAGA CTTCCCCCCA GCCGCCACCT 
    GCGTCCGGCT TCCCTCAGGG CCACCAGCCT CCGCCTCCAC CACCGATGGG ATATGAGCCA 
    CGCTCCCCAT ACGAGGACGG ACCTAACAGG GATTTCAGCC CTCCTCAGTC CCAGTATGAC 
    GGTGTGTCCC CAATGGGCTA CGATCACCGG CCGCGGCACC CCAAACCTGC ACCGGTCCGA 
    TATGAAGAGC CTCCACCTCC ACCTCCTCCG GTGTCCTACG ATGCACGGTC ACCTTTCGAA 
    CCCGAGCCGC ATGCTTTCCC AGGCAACACA CATCGCTCGC CAGACCCACC GAAACAGTAT 
    TATGGAGACG CCGCTATGAG GCCCTTGTAC AACCCTGGAC CTCCGAACAG AGTGTACAAA 
    CATGAGCCGG TGAACTCTGA GCCTCCTGTA CCGCCTCCCA AACCTGAATC TGTCCTGTCT 
    CCAGGAGAAC CGCCCCATTC TGCAGCTTCC AAACCATTGC CGCCTCCTCC CAGAGATGAT 
    GACGATGATG ATGACGATGA TGATGACCCT GCCATGAAGC CCCAGTCTGT GCTCAACAGG 
    GTCAAAATGT TCGAAAACAA ACGCTCTGTG TCGGTAGACC GAGCCAAGGA CGCACCTGAC 
    GTCACTGGAA TTAGGCCCAC AGATCTCCCC AAACCTGCGA GCACCCCTGG ACCTGTGCTC 
    AAAGCCAACT CTCTCAGCAA CCTGGAGCAG GACAAACCCT CCTACAGAGC TCCAGAGCCG 
    CAGAAACCTC AGACGCGTGT TGGGGACGAT GTGGTTCGCT CCAACCACTA CGACCCTGAT 
    GATGATGAAG AGTACTACAG AAAACAGCTC TCATTTTTCG ACCGCCGCAG TTTCGACAGT 
    AAGGTCATGA ATCCACCCAG CGCTGGAGTA AACCGCTTCC ATGAGCCGCC CAAACCACCC 
    CAGACACAGA TCGCTTATCC TTTCGCTAGG ACGGAGTCTG TGGAGAAGGT GAGTCCTGTG 
    GACAAGAGAT ACGAGCCTCT GCCGCCAGTC AACCCTTCAC CTGCTCCATA CAGCCAGAAC 
    ACACCTGTCG CTCCGCCCAC ATCCCTGCCC AAACTCACCA ACATCGAGGT GAACTCTCTG 
    CCAGATCCTC ACAGCTCTCC CAAAGCCAAA CCGGACCTGT CTGCTCTGAG AGCTCCGACC 
    CGGGACGAGC CCATCCAGAG CAGCTACTTG CCCCCCAAGT CCACCATCAA TGGCATGGAT 
    GCTCCTCCCA AAACACTCGG CGTGCCGACC AGCTACAACC GCTACGTCCC CAAGCCTTAC 
    ACCAGCTCGG CGCGACCCTT CGAGCGCAAG TTCGAGAGTC CGAAATTCAA CCACAACCTG 
    CTTCCCAACG ACACGCAGTC CAAACCCAGT GTCAACAACA ACCTGAAGCC TCAGATCTCC 
    CCACAACCCC AGGACACGGA CAGCGGCGTC GACACCTTCA CTCGCACCAT GGACAACAGG 
    CCAAAATATC AGCACAACAA CATCAACGCC ATTCCCAAAG CCGTCCCAGT CAGCCCCAGT 
    GCACTGGATG ATGATGAAGA AGATGAGGGA CACACAGTTG TGGCCACGGC GCGGGGCATC 
    TTTAACTGTA ATGGCGGGGT GTTGAGCTCT ATAGAGACGG GCGTCAGCAT CATTATCCCA 
    CAGGGAGCCA TTCCTGATAG TGTGGAGCAG GAGATCTACT TCAAAGTGTG CCGGGACAAC 
    AGCATCCTGC CTCCTCTGGA TAAGGAGAAA GGTGAAACTC TGCTGAGTCC GCTGGTGATG 
    TGTGGTCCTC ACGGCCTGAA GTTCCTGAAG CCTGTGGAGC TGCGCTTGCC ACACTGTGCG 
    TCTATGACCC CTGATGGTTG GTCTTTTGCT CTAAAATCCT CCGACTCCTC GTCGGGTGAC 
    CCCAAATGCT GGCAAAACAA GTGTCTACCA GGAGACCCCA ATTACCTGGT GGGCGCAAAC 
    TGTGTGTCTG TGCTGATCGA CCACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296407.1
    CDS115..6204
    Translation

    Target ORF information:

    RefSeq Version XM_009298132.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298132.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGGGCCAC AGGGCCGACG GCTCAGAGAA CGCGGCCAGT TCACAGGAGC 
    CGTGATGAAG ACAGGATCTC CAAGGCCGGG CCGCTTCCTC TACCTGCTAA GATGGTGGAG 
    GAGATGCCGA AAGAGCAGAG CGCAGCCAGA GAAACAGAGA AACAGCTCCC CCCTCTGCCA 
    GAGCCCAAAC CTGTGTACGC TCAGCCGGGC CAGCCGGACG TGGACCTGCC TGTTAGTCCG 
    GCAGATGCTC CTGTGCCCAG CGTCACACAT GATGACAGCA TCTTACGGCC GAGCATGAAG 
    CTGGTGAAGT TCAAGAAGGG CGAGAGCGTG GGCTTGAGGC TCGCTGGGGG GAACGACGTT 
    GGGATATTTG TGGCGGGAGT TCTGGAGGAC AGTCCTGCTG CTAAAGAGGG CCTGGAAGAG 
    GGAGACCAGA TTCTCAGGGT AAATAATGTG GATTTTGCAA ATATCATCCG AGAGGAGGCA 
    GTGCTGTTCC TGCTGGATCT GCCCAAAGGA GAGGAGGTCA CCATCCTGGC CCAGAAGAAG 
    AAAGACGTGT ACCGCCGCAT TGTAGAGTCT GACGTGGGAG ACTCGTTCTA CATCAGGACT 
    CATTTTGAAT ATGAGAAGGA GTCGCCCTAT GGCCTGAGCT TCAATAAGGG GGAGGTCTTC 
    AGAGTCGTCG ACACCCTTTA CAATGGCAAA CTGGGCTCCT GGCTCGCCAT CCGCATCGGC 
    AAAAACCACC AGGAGGTGGA GCGGGGCATC ATCCCCAACA AGAACAGGGC TGAGCAGTTA 
    TCAAGTGTCC AGTACACTCT CCCAAAGACT CCAGGAGGGG ATCGCGCCGA CTTCTGGAGA 
    TTCAGAGGCC TCCGCAGCTC CAAACGAAAC CTGAGGAAGA GCAGAGAAGA CCTGTCTGCT 
    CAGCCGGTCC AAACCAAATT CCCTGCTTAT GAAAGAGTGG TGCTTAGAGA AGCTGGTTTC 
    TTGAGGCCAG TGGTCATTTT CGGACCGATC GCGGATGTGG CTAGAGAGAA GCTGTCCAGA 
    GAGGAGACTG ACCTCTTCGA ACTTGCAAAG AGTGAACCCA GAGATGCGGG CACAGACCAG 
    CGCAGCTCCG GAATCATCCG TCTCCATACC ATTAAACAGA TCATTGACCG AGACAAGCAC 
    GCGGTGCTGG ACATCACCCC TAATGCGGTG GACCGTCTGA ACTACGCTCA GTGGTACCCC 
    ATCGTGGTGT TTCTGAATCC AGACAGCAAA CAGGGCGTCA AGAACATGAG GACCAGGCTC 
    TGTCCCGAGT CCAGGAAGAG CGCCCGAAAG CTCTACGAGC GTGCTCTCAA ACTGAGGAAG 
    AACAACCATC ACCTTTTCAC CACCACTATT AACTTGAACA ACATGAATGA CGGGTGGTAC 
    GGTGCTTTGA AAGAGAGCAT CCAGCAGCAG CAGAACCAGT TGGTCTGGGT CTCAGAGGGC 
    AAGGCCGACG GCGCTCCTGA CGATGACCTT GACATCCATG ACGACCGCCT CTCCTACCTC 
    TCGGCTCCGG GCAGCGAGTA CAGCATGTAC AGCACCGACA GCCGCCACAC GTCCGACTAT 
    GAGGACACCG ACACGGAGGG TGGTGCCTAC ACCGACCAGG AGCTGGACGA GACCATGAAC 
    GATGAGGTGG GCCTGCCTAG CGAGCCTGCC ATCACACGCT CATCTGAGCC TGTGCGAGAG 
    GACCCGCCAG TCATTCAGGA CGCAGCTGGA TACCCCAGCT ACCAGCATGC GGTACCGCAA 
    CCTGAAGCGG TGAGCCGCAT TGACCCGGCC GGGTTTAAGA TGGCAGCGCC GCAGCAGCAA 
    GCTGAGGCTG CTCTGGCCTC GCCGCCCCCT CCCTCTGCAG CGGTCCAGCC CACCGCAACA 
    CTAGCGGGTA CGAACCCCGA GGAGCCGCCT GGCACCCCTC AGGCCGACTC CCTTAACAGC 
    CCCGTCCCTG TTCCCGACCC CCAGCCCGAA CCTGAGCTCG CTCAGCCCCC AACACACGAC 
    CCCCACCAGT CGCTTCCTCC TGGCCCAGAT CCAAAGATGT ATAAAAAGGA TCTGTACAGC 
    ATGGACGAGC CGGTGCGAGT GACCCACGGC GTGAAGCCTC CCCCTCCCCA GCAGCAGCAG 
    CAGCCTTTAG GCCCCCCAAC CTCGCTCTCC TACAGCCACC AGCCTGTCTA CCAGGACCAA 
    CAGCCATACC GCCAGTACGA CCACCCGCCT TATGGCTATG ACGGTGGCGG CTACGCACAA 
    CCAAAGCCTC ACAACTACGA CCCGCACCTG CACTACGACA ACCGTGTGCC TCACTATAAC 
    GAGCAGTGGC CCACGTACGA CCAGCAGCAG CAGCAGCAGA CTTCCCCCCA GCCGCCACCT 
    GCGTCCGGCT TCCCTCAGGG CCACCAGCCT CCGCCTCCAC CACCGATGGG ATATGAGCCA 
    CGCTCCCCAT ACGAGGACGG ACCTAACAGG GATTTCAGCC CTCCTCAGTC CCAGTATGAC 
    GGTGTGTCCC CAATGGGCTA CGATCACCGG CCGCGGCACC CCAAACCTGC ACCGGTCCGA 
    TATGAAGAGC CTCCACCTCC ACCTCCTCCG GTGTCCTACG ATGCACGGTC ACCTTTCGAA 
    CCCGAGCCGC ATGCTTTCCC AGGCAACACA CATCGCTCGC CAGACCCACC GAAACAGTAT 
    TATGGAGACG CCGCTATGAG GCCCTTGTAC AACCCTGGAC CTCCGAACAG AGTGTACAAA 
    CATGAGCCGG TGAACTCTGA GCCTCCTGTA CCGCCTCCCA AACCTGAATC TGTCCTGTCT 
    CCAGGAGAAC CGCCCCATTC TGCAGCTTCC AAACCATTGC CGCCTCCTCC CAGAGATGAT 
    GACGATGATG ATGACGATGA TGATGACCCT GCCATGAAGC CCCAGTCTGT GCTCAACAGG 
    GTCAAAATGT TCGAAAACAA ACGCTCTGTG TCGGTAGACC GAGCCAAGGA CGCACCTGAC 
    GTCACTGGAA TTAGGCCCAC AGATCTCCCC AAACCTGCGA GCACCCCTGG ACCTGTGCTC 
    AAAGCCAACT CTCTCAGCAA CCTGGAGCAG GACAAACCCT CCTACAGAGC TCCAGAGCCG 
    CAGAAACCTC AGACGCGTGT TGGGGACGAT GTGGTTCGCT CCAACCACTA CGACCCTGAT 
    GATGATGAAG AGTACTACAG AAAACAGCTC TCATTTTTCG ACCGCCGCAG TTTCGACAGT 
    AAGGTCATGA ATCCACCCAG CGCTGGAGTA AACCGCTTCC ATGAGCCGCC CAAACCACCC 
    CAGACACAGA TCGCTTATCC TTTCGCTAGG ACGGAGTCTG TGGAGAAGGT GAGTCCTGTG 
    GACAAGAGAT ACGAGCCTCT GCCGCCAGTC AACCCTTCAC CTGCTCCATA CAGCCAGAAC 
    ACACCTGTCG CTCCGCCCAC ATCCCTGCCC AAACTCACCA ACATCGAGGT GAACTCTCTG 
    CCAGATCCTC ACAGCTCTCC CAAAGCCAAA CCGGACCTGT CTGCTCTGAG AGCTCCGACC 
    CGGGACGAGC CCATCCAGAG CAGCTACTTG CCCCCCAAGT CCACCATCAA TGGCATGGAT 
    GCTCCTCCCA AAACACTCGG CGTGCCGACC AGCTACAACC GCTACGTCCC CAAGCCTTAC 
    ACCAGCTCGG CGCGACCCTT CGAGCGCAAG TTCGAGAGTC CGAAATTCAA CCACAACCTG 
    CTTCCCAACG ACACGCAGTC CAAACCCAGT GTCAACAACA ACCTGAAGCC TCAGATCTCC 
    CCACAACCCC AGGACACGGA CAGCGGCGTC GACACCTTCA CTCGCACCAT GGACAACAGG 
    CCAAAATATC AGCACAACAA CATCAACGCC ATTCCCAAAG CCGTCCCAGT CAGCCCCAGT 
    GCACTGGATG ATGATGAAGA AGATGAGGGA CACACAGTTG TGGCCACGGC GCGGGGCATC 
    TTTAACTGTA ATGGCGGGGT GTTGAGCTCT ATAGAGACGG GCGTCAGCAT CATTATCCCA 
    CAGGGAGCCA TTCCTGATAG TGTGGAGCAG GAGATCTACT TCAAAGTGTG CCGGGACAAC 
    AGCATCCTGC CTCCTCTGGA TAAGGAGAAA GGTGAAACTC TGCTGAGTCC GCTGGTGATG 
    TGTGGTCCTC ACGGCCTGAA GTTCCTGAAG CCTGTGGAGC TGCGCTTGCC ACACTGTGCG 
    TCTATGACCC CTGATGGTTG GTCTTTTGCT CTAAAATCCT CCGACTCCTC GTCGGGTGAC 
    CCCAAATGCT GGCAAAACAA GTGTCTACCA GGAGACCCCA ATTACCTGGT GGGCGCAAAC 
    TGTGTGTCTG TGCTGATCGA CCACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_009298132.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296407.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID ODa44622
    Clone ID Related Accession (Same CDS sequence) XM_009298132.2 , XM_009298132.3
    Accession Version XM_009298132.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298132.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGGGCCAC AGGGCCGACG GCTCAGAGAA CGCGGCCAGT TCACAGGAGC 
    CGTGATGAAG ACAGGATCTC CAAGGCCGGG CCGCTTCCTC TACCTGCTAA GATGGTGGAG 
    GAGATGCCGA AAGAGCAGAG CGCAGCCAGA GAAACAGAGA AACAGCTCCC CCCTCTGCCA 
    GAGCCCAAAC CTGTGTACGC TCAGCCGGGC CAGCCGGACG TGGACCTGCC TGTTAGTCCG 
    GCAGATGCTC CTGTGCCCAG CGTCACACAT GATGACAGCA TCTTACGGCC GAGCATGAAG 
    CTGGTGAAGT TCAAGAAGGG CGAGAGCGTG GGCTTGAGGC TCGCTGGGGG GAACGACGTT 
    GGGATATTTG TGGCGGGAGT TCTGGAGGAC AGTCCTGCTG CTAAAGAGGG CCTGGAAGAG 
    GGAGACCAGA TTCTCAGGGT AAATAATGTG GATTTTGCAA ATATCATCCG AGAGGAGGCA 
    GTGCTGTTCC TGCTGGATCT GCCCAAAGGA GAGGAGGTCA CCATCCTGGC CCAGAAGAAG 
    AAAGACGTGT ACCGCCGCAT TGTAGAGTCT GACGTGGGAG ACTCGTTCTA CATCAGGACT 
    CATTTTGAAT ATGAGAAGGA GTCGCCCTAT GGCCTGAGCT TCAATAAGGG GGAGGTCTTC 
    AGAGTCGTCG ACACCCTTTA CAATGGCAAA CTGGGCTCCT GGCTCGCCAT CCGCATCGGC 
    AAAAACCACC AGGAGGTGGA GCGGGGCATC ATCCCCAACA AGAACAGGGC TGAGCAGTTA 
    TCAAGTGTCC AGTACACTCT CCCAAAGACT CCAGGAGGGG ATCGCGCCGA CTTCTGGAGA 
    TTCAGAGGCC TCCGCAGCTC CAAACGAAAC CTGAGGAAGA GCAGAGAAGA CCTGTCTGCT 
    CAGCCGGTCC AAACCAAATT CCCTGCTTAT GAAAGAGTGG TGCTTAGAGA AGCTGGTTTC 
    TTGAGGCCAG TGGTCATTTT CGGACCGATC GCGGATGTGG CTAGAGAGAA GCTGTCCAGA 
    GAGGAGACTG ACCTCTTCGA ACTTGCAAAG AGTGAACCCA GAGATGCGGG CACAGACCAG 
    CGCAGCTCCG GAATCATCCG TCTCCATACC ATTAAACAGA TCATTGACCG AGACAAGCAC 
    GCGGTGCTGG ACATCACCCC TAATGCGGTG GACCGTCTGA ACTACGCTCA GTGGTACCCC 
    ATCGTGGTGT TTCTGAATCC AGACAGCAAA CAGGGCGTCA AGAACATGAG GACCAGGCTC 
    TGTCCCGAGT CCAGGAAGAG CGCCCGAAAG CTCTACGAGC GTGCTCTCAA ACTGAGGAAG 
    AACAACCATC ACCTTTTCAC CACCACTATT AACTTGAACA ACATGAATGA CGGGTGGTAC 
    GGTGCTTTGA AAGAGAGCAT CCAGCAGCAG CAGAACCAGT TGGTCTGGGT CTCAGAGGGC 
    AAGGCCGACG GCGCTCCTGA CGATGACCTT GACATCCATG ACGACCGCCT CTCCTACCTC 
    TCGGCTCCGG GCAGCGAGTA CAGCATGTAC AGCACCGACA GCCGCCACAC GTCCGACTAT 
    GAGGACACCG ACACGGAGGG TGGTGCCTAC ACCGACCAGG AGCTGGACGA GACCATGAAC 
    GATGAGGTGG GCCTGCCTAG CGAGCCTGCC ATCACACGCT CATCTGAGCC TGTGCGAGAG 
    GACCCGCCAG TCATTCAGGA CGCAGCTGGA TACCCCAGCT ACCAGCATGC GGTACCGCAA 
    CCTGAAGCGG TGAGCCGCAT TGACCCGGCC GGGTTTAAGA TGGCAGCGCC GCAGCAGCAA 
    GCTGAGGCTG CTCTGGCCTC GCCGCCCCCT CCCTCTGCAG CGGTCCAGCC CACCGCAACA 
    CTAGCGGGTA CGAACCCCGA GGAGCCGCCT GGCACCCCTC AGGCCGACTC CCTTAACAGC 
    CCCGTCCCTG TTCCCGACCC CCAGCCCGAA CCTGAGCTCG CTCAGCCCCC AACACACGAC 
    CCCCACCAGT CGCTTCCTCC TGGCCCAGAT CCAAAGATGT ATAAAAAGGA TCTGTACAGC 
    ATGGACGAGC CGGTGCGAGT GACCCACGGC GTGAAGCCTC CCCCTCCCCA GCAGCAGCAG 
    CAGCCTTTAG GCCCCCCAAC CTCGCTCTCC TACAGCCACC AGCCTGTCTA CCAGGACCAA 
    CAGCCATACC GCCAGTACGA CCACCCGCCT TATGGCTATG ACGGTGGCGG CTACGCACAA 
    CCAAAGCCTC ACAACTACGA CCCGCACCTG CACTACGACA ACCGTGTGCC TCACTATAAC 
    GAGCAGTGGC CCACGTACGA CCAGCAGCAG CAGCAGCAGA CTTCCCCCCA GCCGCCACCT 
    GCGTCCGGCT TCCCTCAGGG CCACCAGCCT CCGCCTCCAC CACCGATGGG ATATGAGCCA 
    CGCTCCCCAT ACGAGGACGG ACCTAACAGG GATTTCAGCC CTCCTCAGTC CCAGTATGAC 
    GGTGTGTCCC CAATGGGCTA CGATCACCGG CCGCGGCACC CCAAACCTGC ACCGGTCCGA 
    TATGAAGAGC CTCCACCTCC ACCTCCTCCG GTGTCCTACG ATGCACGGTC ACCTTTCGAA 
    CCCGAGCCGC ATGCTTTCCC AGGCAACACA CATCGCTCGC CAGACCCACC GAAACAGTAT 
    TATGGAGACG CCGCTATGAG GCCCTTGTAC AACCCTGGAC CTCCGAACAG AGTGTACAAA 
    CATGAGCCGG TGAACTCTGA GCCTCCTGTA CCGCCTCCCA AACCTGAATC TGTCCTGTCT 
    CCAGGAGAAC CGCCCCATTC TGCAGCTTCC AAACCATTGC CGCCTCCTCC CAGAGATGAT 
    GACGATGATG ATGACGATGA TGATGACCCT GCCATGAAGC CCCAGTCTGT GCTCAACAGG 
    GTCAAAATGT TCGAAAACAA ACGCTCTGTG TCGGTAGACC GAGCCAAGGA CGCACCTGAC 
    GTCACTGGAA TTAGGCCCAC AGATCTCCCC AAACCTGCGA GCACCCCTGG ACCTGTGCTC 
    AAAGCCAACT CTCTCAGCAA CCTGGAGCAG GACAAACCCT CCTACAGAGC TCCAGAGCCG 
    CAGAAACCTC AGACGCGTGT TGGGGACGAT GTGGTTCGCT CCAACCACTA CGACCCTGAT 
    GATGATGAAG AGTACTACAG AAAACAGCTC TCATTTTTCG ACCGCCGCAG TTTCGACAGT 
    AAGGTCATGA ATCCACCCAG CGCTGGAGTA AACCGCTTCC ATGAGCCGCC CAAACCACCC 
    CAGACACAGA TCGCTTATCC TTTCGCTAGG ACGGAGTCTG TGGAGAAGGT GAGTCCTGTG 
    GACAAGAGAT ACGAGCCTCT GCCGCCAGTC AACCCTTCAC CTGCTCCATA CAGCCAGAAC 
    ACACCTGTCG CTCCGCCCAC ATCCCTGCCC AAACTCACCA ACATCGAGGT GAACTCTCTG 
    CCAGATCCTC ACAGCTCTCC CAAAGCCAAA CCGGACCTGT CTGCTCTGAG AGCTCCGACC 
    CGGGACGAGC CCATCCAGAG CAGCTACTTG CCCCCCAAGT CCACCATCAA TGGCATGGAT 
    GCTCCTCCCA AAACACTCGG CGTGCCGACC AGCTACAACC GCTACGTCCC CAAGCCTTAC 
    ACCAGCTCGG CGCGACCCTT CGAGCGCAAG TTCGAGAGTC CGAAATTCAA CCACAACCTG 
    CTTCCCAACG ACACGCAGTC CAAACCCAGT GTCAACAACA ACCTGAAGCC TCAGATCTCC 
    CCACAACCCC AGGACACGGA CAGCGGCGTC GACACCTTCA CTCGCACCAT GGACAACAGG 
    CCAAAATATC AGCACAACAA CATCAACGCC ATTCCCAAAG CCGTCCCAGT CAGCCCCAGT 
    GCACTGGATG ATGATGAAGA AGATGAGGGA CACACAGTTG TGGCCACGGC GCGGGGCATC 
    TTTAACTGTA ATGGCGGGGT GTTGAGCTCT ATAGAGACGG GCGTCAGCAT CATTATCCCA 
    CAGGGAGCCA TTCCTGATAG TGTGGAGCAG GAGATCTACT TCAAAGTGTG CCGGGACAAC 
    AGCATCCTGC CTCCTCTGGA TAAGGAGAAA GGTGAAACTC TGCTGAGTCC GCTGGTGATG 
    TGTGGTCCTC ACGGCCTGAA GTTCCTGAAG CCTGTGGAGC TGCGCTTGCC ACACTGTGCG 
    TCTATGACCC CTGATGGTTG GTCTTTTGCT CTAAAATCCT CCGACTCCTC GTCGGGTGAC 
    CCCAAATGCT GGCAAAACAA GTGTCTACCA GGAGACCCCA ATTACCTGGT GGGCGCAAAC 
    TGTGTGTCTG TGCTGATCGA CCACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296407.1
    CDS110..6199
    Translation

    Target ORF information:

    RefSeq Version XM_009298132.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298132.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGGGCCAC AGGGCCGACG GCTCAGAGAA CGCGGCCAGT TCACAGGAGC 
    CGTGATGAAG ACAGGATCTC CAAGGCCGGG CCGCTTCCTC TACCTGCTAA GATGGTGGAG 
    GAGATGCCGA AAGAGCAGAG CGCAGCCAGA GAAACAGAGA AACAGCTCCC CCCTCTGCCA 
    GAGCCCAAAC CTGTGTACGC TCAGCCGGGC CAGCCGGACG TGGACCTGCC TGTTAGTCCG 
    GCAGATGCTC CTGTGCCCAG CGTCACACAT GATGACAGCA TCTTACGGCC GAGCATGAAG 
    CTGGTGAAGT TCAAGAAGGG CGAGAGCGTG GGCTTGAGGC TCGCTGGGGG GAACGACGTT 
    GGGATATTTG TGGCGGGAGT TCTGGAGGAC AGTCCTGCTG CTAAAGAGGG CCTGGAAGAG 
    GGAGACCAGA TTCTCAGGGT AAATAATGTG GATTTTGCAA ATATCATCCG AGAGGAGGCA 
    GTGCTGTTCC TGCTGGATCT GCCCAAAGGA GAGGAGGTCA CCATCCTGGC CCAGAAGAAG 
    AAAGACGTGT ACCGCCGCAT TGTAGAGTCT GACGTGGGAG ACTCGTTCTA CATCAGGACT 
    CATTTTGAAT ATGAGAAGGA GTCGCCCTAT GGCCTGAGCT TCAATAAGGG GGAGGTCTTC 
    AGAGTCGTCG ACACCCTTTA CAATGGCAAA CTGGGCTCCT GGCTCGCCAT CCGCATCGGC 
    AAAAACCACC AGGAGGTGGA GCGGGGCATC ATCCCCAACA AGAACAGGGC TGAGCAGTTA 
    TCAAGTGTCC AGTACACTCT CCCAAAGACT CCAGGAGGGG ATCGCGCCGA CTTCTGGAGA 
    TTCAGAGGCC TCCGCAGCTC CAAACGAAAC CTGAGGAAGA GCAGAGAAGA CCTGTCTGCT 
    CAGCCGGTCC AAACCAAATT CCCTGCTTAT GAAAGAGTGG TGCTTAGAGA AGCTGGTTTC 
    TTGAGGCCAG TGGTCATTTT CGGACCGATC GCGGATGTGG CTAGAGAGAA GCTGTCCAGA 
    GAGGAGACTG ACCTCTTCGA ACTTGCAAAG AGTGAACCCA GAGATGCGGG CACAGACCAG 
    CGCAGCTCCG GAATCATCCG TCTCCATACC ATTAAACAGA TCATTGACCG AGACAAGCAC 
    GCGGTGCTGG ACATCACCCC TAATGCGGTG GACCGTCTGA ACTACGCTCA GTGGTACCCC 
    ATCGTGGTGT TTCTGAATCC AGACAGCAAA CAGGGCGTCA AGAACATGAG GACCAGGCTC 
    TGTCCCGAGT CCAGGAAGAG CGCCCGAAAG CTCTACGAGC GTGCTCTCAA ACTGAGGAAG 
    AACAACCATC ACCTTTTCAC CACCACTATT AACTTGAACA ACATGAATGA CGGGTGGTAC 
    GGTGCTTTGA AAGAGAGCAT CCAGCAGCAG CAGAACCAGT TGGTCTGGGT CTCAGAGGGC 
    AAGGCCGACG GCGCTCCTGA CGATGACCTT GACATCCATG ACGACCGCCT CTCCTACCTC 
    TCGGCTCCGG GCAGCGAGTA CAGCATGTAC AGCACCGACA GCCGCCACAC GTCCGACTAT 
    GAGGACACCG ACACGGAGGG TGGTGCCTAC ACCGACCAGG AGCTGGACGA GACCATGAAC 
    GATGAGGTGG GCCTGCCTAG CGAGCCTGCC ATCACACGCT CATCTGAGCC TGTGCGAGAG 
    GACCCGCCAG TCATTCAGGA CGCAGCTGGA TACCCCAGCT ACCAGCATGC GGTACCGCAA 
    CCTGAAGCGG TGAGCCGCAT TGACCCGGCC GGGTTTAAGA TGGCAGCGCC GCAGCAGCAA 
    GCTGAGGCTG CTCTGGCCTC GCCGCCCCCT CCCTCTGCAG CGGTCCAGCC CACCGCAACA 
    CTAGCGGGTA CGAACCCCGA GGAGCCGCCT GGCACCCCTC AGGCCGACTC CCTTAACAGC 
    CCCGTCCCTG TTCCCGACCC CCAGCCCGAA CCTGAGCTCG CTCAGCCCCC AACACACGAC 
    CCCCACCAGT CGCTTCCTCC TGGCCCAGAT CCAAAGATGT ATAAAAAGGA TCTGTACAGC 
    ATGGACGAGC CGGTGCGAGT GACCCACGGC GTGAAGCCTC CCCCTCCCCA GCAGCAGCAG 
    CAGCCTTTAG GCCCCCCAAC CTCGCTCTCC TACAGCCACC AGCCTGTCTA CCAGGACCAA 
    CAGCCATACC GCCAGTACGA CCACCCGCCT TATGGCTATG ACGGTGGCGG CTACGCACAA 
    CCAAAGCCTC ACAACTACGA CCCGCACCTG CACTACGACA ACCGTGTGCC TCACTATAAC 
    GAGCAGTGGC CCACGTACGA CCAGCAGCAG CAGCAGCAGA CTTCCCCCCA GCCGCCACCT 
    GCGTCCGGCT TCCCTCAGGG CCACCAGCCT CCGCCTCCAC CACCGATGGG ATATGAGCCA 
    CGCTCCCCAT ACGAGGACGG ACCTAACAGG GATTTCAGCC CTCCTCAGTC CCAGTATGAC 
    GGTGTGTCCC CAATGGGCTA CGATCACCGG CCGCGGCACC CCAAACCTGC ACCGGTCCGA 
    TATGAAGAGC CTCCACCTCC ACCTCCTCCG GTGTCCTACG ATGCACGGTC ACCTTTCGAA 
    CCCGAGCCGC ATGCTTTCCC AGGCAACACA CATCGCTCGC CAGACCCACC GAAACAGTAT 
    TATGGAGACG CCGCTATGAG GCCCTTGTAC AACCCTGGAC CTCCGAACAG AGTGTACAAA 
    CATGAGCCGG TGAACTCTGA GCCTCCTGTA CCGCCTCCCA AACCTGAATC TGTCCTGTCT 
    CCAGGAGAAC CGCCCCATTC TGCAGCTTCC AAACCATTGC CGCCTCCTCC CAGAGATGAT 
    GACGATGATG ATGACGATGA TGATGACCCT GCCATGAAGC CCCAGTCTGT GCTCAACAGG 
    GTCAAAATGT TCGAAAACAA ACGCTCTGTG TCGGTAGACC GAGCCAAGGA CGCACCTGAC 
    GTCACTGGAA TTAGGCCCAC AGATCTCCCC AAACCTGCGA GCACCCCTGG ACCTGTGCTC 
    AAAGCCAACT CTCTCAGCAA CCTGGAGCAG GACAAACCCT CCTACAGAGC TCCAGAGCCG 
    CAGAAACCTC AGACGCGTGT TGGGGACGAT GTGGTTCGCT CCAACCACTA CGACCCTGAT 
    GATGATGAAG AGTACTACAG AAAACAGCTC TCATTTTTCG ACCGCCGCAG TTTCGACAGT 
    AAGGTCATGA ATCCACCCAG CGCTGGAGTA AACCGCTTCC ATGAGCCGCC CAAACCACCC 
    CAGACACAGA TCGCTTATCC TTTCGCTAGG ACGGAGTCTG TGGAGAAGGT GAGTCCTGTG 
    GACAAGAGAT ACGAGCCTCT GCCGCCAGTC AACCCTTCAC CTGCTCCATA CAGCCAGAAC 
    ACACCTGTCG CTCCGCCCAC ATCCCTGCCC AAACTCACCA ACATCGAGGT GAACTCTCTG 
    CCAGATCCTC ACAGCTCTCC CAAAGCCAAA CCGGACCTGT CTGCTCTGAG AGCTCCGACC 
    CGGGACGAGC CCATCCAGAG CAGCTACTTG CCCCCCAAGT CCACCATCAA TGGCATGGAT 
    GCTCCTCCCA AAACACTCGG CGTGCCGACC AGCTACAACC GCTACGTCCC CAAGCCTTAC 
    ACCAGCTCGG CGCGACCCTT CGAGCGCAAG TTCGAGAGTC CGAAATTCAA CCACAACCTG 
    CTTCCCAACG ACACGCAGTC CAAACCCAGT GTCAACAACA ACCTGAAGCC TCAGATCTCC 
    CCACAACCCC AGGACACGGA CAGCGGCGTC GACACCTTCA CTCGCACCAT GGACAACAGG 
    CCAAAATATC AGCACAACAA CATCAACGCC ATTCCCAAAG CCGTCCCAGT CAGCCCCAGT 
    GCACTGGATG ATGATGAAGA AGATGAGGGA CACACAGTTG TGGCCACGGC GCGGGGCATC 
    TTTAACTGTA ATGGCGGGGT GTTGAGCTCT ATAGAGACGG GCGTCAGCAT CATTATCCCA 
    CAGGGAGCCA TTCCTGATAG TGTGGAGCAG GAGATCTACT TCAAAGTGTG CCGGGACAAC 
    AGCATCCTGC CTCCTCTGGA TAAGGAGAAA GGTGAAACTC TGCTGAGTCC GCTGGTGATG 
    TGTGGTCCTC ACGGCCTGAA GTTCCTGAAG CCTGTGGAGC TGCGCTTGCC ACACTGTGCG 
    TCTATGACCC CTGATGGTTG GTCTTTTGCT CTAAAATCCT CCGACTCCTC GTCGGGTGAC 
    CCCAAATGCT GGCAAAACAA GTGTCTACCA GGAGACCCCA ATTACCTGGT GGGCGCAAAC 
    TGTGTGTCTG TGCTGATCGA CCACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470730.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326405.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID ODa44623
    Clone ID Related Accession (Same CDS sequence) XM_021470730.1
    Accession Version XM_021470730.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6087bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGAAGCTGAT CGAGAGGTCA 
    AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
    GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGATAT TTCAGACATC 
    CACTCCGTGG CCTCTGATCA TTCCAACCGA TCCCACGACC GGAAGAGGAG CAGCCGCTCT 
    CGTTCTCCAG ACCGCCGCTC CGAACCCTCA GACCACTCCA GACACTCTCC ACCACAGATC 
    AGCAACGGCA GGGCCACAGG GCCGACGGCT CAGAGAACGC GGCCAGTTCA CAGGAGCCGT 
    GATGAAGACA GGATCTCCAA GGCCGGGCCG CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG 
    ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG 
    CCCAAACCTG TGTACGCTCA GCCGGGCCAG CCGGACGTGG ACCTGCCTGT TAGTCCGGCA 
    GATGCTCCTG TGCCCAGCGT CACACATGAT GACAGCATCT TACGGCCGAG CATGAAGCTG 
    GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC TTGAGGCTCG CTGGGGGGAA CGACGTTGGG 
    ATATTTGTGG CGGGAGTTCT GGAGGACAGT CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA 
    GACCAGATTC TCAGGGTAAA TAATGTGGAT TTTGCAAATA TCATCCGAGA GGAGGCAGTG 
    CTGTTCCTGC TGGATCTGCC CAAAGGAGAG GAGGTCACCA TCCTGGCCCA GAAGAAGAAA 
    GACGTGTACC GCCGCATTGT AGAGTCTGAC GTGGGAGACT CGTTCTACAT CAGGACTCAT 
    TTTGAATATG AGAAGGAGTC GCCCTATGGC CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA 
    GTCGTCGACA CCCTTTACAA TGGCAAACTG GGCTCCTGGC TCGCCATCCG CATCGGCAAA 
    AACCACCAGG AGGTGGAGCG GGGCATCATC CCCAACAAGA ACAGGGCTGA GCAGTTATCA 
    AGTGTCCAGT ACACTCTCCC AAAGACTCCA GGAGGGGATC GCGCCGACTT CTGGAGATTC 
    AGAGGCCTCC GCAGCTCCAA ACGAAACCTG AGGAAGAGCA GAGAAGACCT GTCTGCTCAG 
    CCGGTCCAAA CCAAATTCCC TGCTTATGAA AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG 
    AGGCCAGTGG TCATTTTCGG ACCGATCGCG GATGTGGCTA GAGAGAAGCT GTCCAGAGAG 
    GAGACTGACC TCTTCGAACT TGCAAAGAGT GAACCCAGAG ATGCGGGCAC AGACCAGCGC 
    AGCTCCGGAA TCATCCGTCT CCATACCATT AAACAGATCA TTGACCGAGA CAAGCACGCG 
    GTGCTGGACA TCACCCCTAA TGCGGTGGAC CGTCTGAACT ACGCTCAGTG GTACCCCATC 
    GTGGTGTTTC TGAATCCAGA CAGCAAACAG GGCGTCAAGA ACATGAGGAC CAGGCTCTGT 
    CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC TACGAGCGTG CTCTCAAACT GAGGAAGAAC 
    AACCATCACC TTTTCACCAC CACTATTAAC TTGAACAACA TGAATGACGG GTGGTACGGT 
    GCTTTGAAAG AGAGCATCCA GCAGCAGCAG AACCAGTTGG TCTGGGTCTC AGAGGGCAAG 
    GCCGACGGCG CTCCTGACGA TGACCTTGAC ATCCATGACG ACCGCCTCTC CTACCTCTCG 
    GCTCCGGGCA GCGAGTACAG CATGTACAGC ACCGACAGCC GCCACACGTC CGACTATGAG 
    GACACCGACA CGGAGGGTGG TGCCTACACC GACCAGGAGC TGGACGAGAC CATGAACGAT 
    GAGGTGGGCC TGCCTAGCGA GCCTGCCATC ACACGCTCAT CTGAGCCTGT GCGAGAGGAC 
    CCGCCAGTCA TTCAGGACGC AGCTGGATAC CCCAGCTACC AGCATGCGGT ACCGCAACCT 
    GAAGCGGTGA GCCGCATTGA CCCGGCCGGG TTTAAGATGG CAGCGCCGCA GCAGCAAGCT 
    GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC TCTGCAGCGG TCCAGCCCAC CGCAACACTA 
    GCGGGTACGA ACCCCGAGGA GCCGCCTGGC ACCCCTCAGG CCGACTCCCT TAACAGCCCC 
    GTCCCTGTTC CCGACCCCCA GCCCGAACCT GAGCTCGCTC AGCCCCCAAC ACACGACCCC 
    CACCAGTCGC TTCCTCCTGG CCCAGATCCA AAGATGTATA AAAAGGATCT GTACAGCATG 
    GACGAGCCGG TGCGAGTGAC CCACGGCGTG AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG 
    CCTTTAGGCC CCCCAACCTC GCTCTCCTAC AGCCACCAGC CTGTCTACCA GGACCAACAG 
    CCATACCGCC AGTACGACCA CCCGCCTTAT GGCTATGACG GTGGCGGCTA CGCACAACCA 
    AAGCCTCACA ACTACGACCC GCACCTGCAC TACGACAACC GTGTGCCTCA CTATAACGAG 
    CAGTGGCCCA CGTACGACCA GCAGCAGCAG CAGCAGACTT CCCCCCAGCC GCCACCTGCG 
    TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG CCTCCACCAC CGATGGGATA TGAGCCACGC 
    TCCCCATACG AGGACGGACC TAACAGGGAT TTCAGCCCTC CTCAGTCCCA GTATGACGGT 
    GTGTCCCCAA TGGGCTACGA TCACCGGCCG CGGCACCCCA AACCTGCACC GGTCCGATAT 
    GAAGAGCCTC CACCTCCACC TCCTCCGGTG TCCTACGATG CACGGTCACC TTTCGAACCC 
    GAGCCGCATG CTTTCCCAGG CAACACACAT CGCTCGCCAG ACCCACCGAA ACAGTATTAT 
    GGAGACGCCG CTATGAGGCC CTTGTACAAC CCTGGACCTC CGAACAGAGT GTACAAACAT 
    GAGCCGGTGA ACTCTGAGCC TCCTGTACCG CCTCCCAAAC CTGAATCTGT CCTGTCTCCA 
    GGAGAACCGC CCCATTCTGC AGCTTCCAAA CCATTGCCGC CTCCTCCCAG AGATGATGAC 
    GATGATGATG ACGATGATGA TGACCCTGCC ATGAAGCCCC AGTCTGTGCT CAACAGGGTC 
    AAAATGTTCG AAAACAAACG CTCTGTGTCG GTAGACCGAG CCAAGGACGC ACCTGACGTC 
    ACTGGAATTA GGCCCACAGA TCTCCCCAAA CCTGCGAGCA CCCCTGGACC TGTGCTCAAA 
    GCCAACTCTC TCAGCAACCT GGAGCAGGAC AAACCCTCCT ACAGAGCTCC AGAGCCGCAG 
    AAACCTCAGA CGCGTGTTGG GGACGATGTG GTTCGCTCCA ACCACTACGA CCCTGATGAT 
    GATGAAGAGT ACTACAGAAA ACAGCTCTCA TTTTTCGACC GCCGCAGTTT CGACAGTAAG 
    GTCATGAATC CACCCAGCGC TGGAGTAAAC CGCTTCCATG AGCCGCCCAA ACCACCCCAG 
    ACACAGATCG CTTATCCTTT CGCTAGGACG GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC 
    AAGAGATACG AGCCTCTGCC GCCAGTCAAC CCTTCACCTG CTCCATACAG CCAGAACACA 
    CCTGTCGCTC CGCCCACATC CCTGCCCAAA CTCACCAACA TCGAGGTGAA CTCTCTGCCA 
    GATCCTCACA GCTCTCCCAA AGCCAAACCG GACCTGTCTG CTCTGAGAGC TCCGACCCGG 
    GACGAGCCCA TCCAGAGCAG CTACTTGCCC CCCAAGTCCA CCATCAATGG CATGGATGCT 
    CCTCCCAAAA CACTCGGCGT GCCGACCAGC TACAACCGCT ACGTCCCCAA GCCTTACACC 
    AGCTCGGCGC GACCCTTCGA GCGCAAGTTC GAGAGTCCGA AATTCAACCA CAACCTGCTT 
    CCCAACGACA CGCAGTCCAA ACCCAGTGTC AACAACAACC TGAAGCCTCA GATCTCCCCA 
    CAACCCCAGG ACACGGACAG CGGCGTCGAC ACCTTCACTC GCACCATGGA CAACAGGCCA 
    AAATATCAGC ACAACAACAT CAACGCCATT CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA 
    CTGGATGATG ATGAAGAAGA TGAGGGACAC ACAGTTGTGG CCACGGCGCG GGGCATCTTT 
    AACTGTAATG GCGGGGTGTT GAGCTCTATA GAGACGGGCG TCAGCATCAT TATCCCACAG 
    GGAGCCATTC CTGATAGTGT GGAGCAGGAG ATCTACTTCA AAGTGTGCCG GGACAACAGC 
    ATCCTGCCTC CTCTGGATAA GGAGAAAGGT GAAACTCTGC TGAGTCCGCT GGTGATGTGT 
    GGTCCTCACG GCCTGAAGTT CCTGAAGCCT GTGGAGCTGC GCTTGCCACA CTGTGCGTCT 
    ATGACCCCTG ATGGTTGGTC TTTTGCTCTA AAATCCTCCG ACTCCTCGTC GGGTGACCCC 
    AAATGCTGGC AAAACAAGTG TCTACCAGGA GACCCCAATT ACCTGGTGGG CGCAAACTGT 
    GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326405.1
    CDS110..6196
    Translation

    Target ORF information:

    RefSeq Version XM_021470730.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470730.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGAAGCTGAT CGAGAGGTCA 
    AAGGGAAAGC TGAAGATGGT GGTGCAGAGA GACGAGCGGG CGACGCTGCT GAACATCCCC 
    GATCTGGACG ACAGCATACC TTCTGCTAAT GCCTCGGACA GAGATGATAT TTCAGACATC 
    CACTCCGTGG CCTCTGATCA TTCCAACCGA TCCCACGACC GGAAGAGGAG CAGCCGCTCT 
    CGTTCTCCAG ACCGCCGCTC CGAACCCTCA GACCACTCCA GACACTCTCC ACCACAGATC 
    AGCAACGGCA GGGCCACAGG GCCGACGGCT CAGAGAACGC GGCCAGTTCA CAGGAGCCGT 
    GATGAAGACA GGATCTCCAA GGCCGGGCCG CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG 
    ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG 
    CCCAAACCTG TGTACGCTCA GCCGGGCCAG CCGGACGTGG ACCTGCCTGT TAGTCCGGCA 
    GATGCTCCTG TGCCCAGCGT CACACATGAT GACAGCATCT TACGGCCGAG CATGAAGCTG 
    GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC TTGAGGCTCG CTGGGGGGAA CGACGTTGGG 
    ATATTTGTGG CGGGAGTTCT GGAGGACAGT CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA 
    GACCAGATTC TCAGGGTAAA TAATGTGGAT TTTGCAAATA TCATCCGAGA GGAGGCAGTG 
    CTGTTCCTGC TGGATCTGCC CAAAGGAGAG GAGGTCACCA TCCTGGCCCA GAAGAAGAAA 
    GACGTGTACC GCCGCATTGT AGAGTCTGAC GTGGGAGACT CGTTCTACAT CAGGACTCAT 
    TTTGAATATG AGAAGGAGTC GCCCTATGGC CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA 
    GTCGTCGACA CCCTTTACAA TGGCAAACTG GGCTCCTGGC TCGCCATCCG CATCGGCAAA 
    AACCACCAGG AGGTGGAGCG GGGCATCATC CCCAACAAGA ACAGGGCTGA GCAGTTATCA 
    AGTGTCCAGT ACACTCTCCC AAAGACTCCA GGAGGGGATC GCGCCGACTT CTGGAGATTC 
    AGAGGCCTCC GCAGCTCCAA ACGAAACCTG AGGAAGAGCA GAGAAGACCT GTCTGCTCAG 
    CCGGTCCAAA CCAAATTCCC TGCTTATGAA AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG 
    AGGCCAGTGG TCATTTTCGG ACCGATCGCG GATGTGGCTA GAGAGAAGCT GTCCAGAGAG 
    GAGACTGACC TCTTCGAACT TGCAAAGAGT GAACCCAGAG ATGCGGGCAC AGACCAGCGC 
    AGCTCCGGAA TCATCCGTCT CCATACCATT AAACAGATCA TTGACCGAGA CAAGCACGCG 
    GTGCTGGACA TCACCCCTAA TGCGGTGGAC CGTCTGAACT ACGCTCAGTG GTACCCCATC 
    GTGGTGTTTC TGAATCCAGA CAGCAAACAG GGCGTCAAGA ACATGAGGAC CAGGCTCTGT 
    CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC TACGAGCGTG CTCTCAAACT GAGGAAGAAC 
    AACCATCACC TTTTCACCAC CACTATTAAC TTGAACAACA TGAATGACGG GTGGTACGGT 
    GCTTTGAAAG AGAGCATCCA GCAGCAGCAG AACCAGTTGG TCTGGGTCTC AGAGGGCAAG 
    GCCGACGGCG CTCCTGACGA TGACCTTGAC ATCCATGACG ACCGCCTCTC CTACCTCTCG 
    GCTCCGGGCA GCGAGTACAG CATGTACAGC ACCGACAGCC GCCACACGTC CGACTATGAG 
    GACACCGACA CGGAGGGTGG TGCCTACACC GACCAGGAGC TGGACGAGAC CATGAACGAT 
    GAGGTGGGCC TGCCTAGCGA GCCTGCCATC ACACGCTCAT CTGAGCCTGT GCGAGAGGAC 
    CCGCCAGTCA TTCAGGACGC AGCTGGATAC CCCAGCTACC AGCATGCGGT ACCGCAACCT 
    GAAGCGGTGA GCCGCATTGA CCCGGCCGGG TTTAAGATGG CAGCGCCGCA GCAGCAAGCT 
    GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC TCTGCAGCGG TCCAGCCCAC CGCAACACTA 
    GCGGGTACGA ACCCCGAGGA GCCGCCTGGC ACCCCTCAGG CCGACTCCCT TAACAGCCCC 
    GTCCCTGTTC CCGACCCCCA GCCCGAACCT GAGCTCGCTC AGCCCCCAAC ACACGACCCC 
    CACCAGTCGC TTCCTCCTGG CCCAGATCCA AAGATGTATA AAAAGGATCT GTACAGCATG 
    GACGAGCCGG TGCGAGTGAC CCACGGCGTG AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG 
    CCTTTAGGCC CCCCAACCTC GCTCTCCTAC AGCCACCAGC CTGTCTACCA GGACCAACAG 
    CCATACCGCC AGTACGACCA CCCGCCTTAT GGCTATGACG GTGGCGGCTA CGCACAACCA 
    AAGCCTCACA ACTACGACCC GCACCTGCAC TACGACAACC GTGTGCCTCA CTATAACGAG 
    CAGTGGCCCA CGTACGACCA GCAGCAGCAG CAGCAGACTT CCCCCCAGCC GCCACCTGCG 
    TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG CCTCCACCAC CGATGGGATA TGAGCCACGC 
    TCCCCATACG AGGACGGACC TAACAGGGAT TTCAGCCCTC CTCAGTCCCA GTATGACGGT 
    GTGTCCCCAA TGGGCTACGA TCACCGGCCG CGGCACCCCA AACCTGCACC GGTCCGATAT 
    GAAGAGCCTC CACCTCCACC TCCTCCGGTG TCCTACGATG CACGGTCACC TTTCGAACCC 
    GAGCCGCATG CTTTCCCAGG CAACACACAT CGCTCGCCAG ACCCACCGAA ACAGTATTAT 
    GGAGACGCCG CTATGAGGCC CTTGTACAAC CCTGGACCTC CGAACAGAGT GTACAAACAT 
    GAGCCGGTGA ACTCTGAGCC TCCTGTACCG CCTCCCAAAC CTGAATCTGT CCTGTCTCCA 
    GGAGAACCGC CCCATTCTGC AGCTTCCAAA CCATTGCCGC CTCCTCCCAG AGATGATGAC 
    GATGATGATG ACGATGATGA TGACCCTGCC ATGAAGCCCC AGTCTGTGCT CAACAGGGTC 
    AAAATGTTCG AAAACAAACG CTCTGTGTCG GTAGACCGAG CCAAGGACGC ACCTGACGTC 
    ACTGGAATTA GGCCCACAGA TCTCCCCAAA CCTGCGAGCA CCCCTGGACC TGTGCTCAAA 
    GCCAACTCTC TCAGCAACCT GGAGCAGGAC AAACCCTCCT ACAGAGCTCC AGAGCCGCAG 
    AAACCTCAGA CGCGTGTTGG GGACGATGTG GTTCGCTCCA ACCACTACGA CCCTGATGAT 
    GATGAAGAGT ACTACAGAAA ACAGCTCTCA TTTTTCGACC GCCGCAGTTT CGACAGTAAG 
    GTCATGAATC CACCCAGCGC TGGAGTAAAC CGCTTCCATG AGCCGCCCAA ACCACCCCAG 
    ACACAGATCG CTTATCCTTT CGCTAGGACG GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC 
    AAGAGATACG AGCCTCTGCC GCCAGTCAAC CCTTCACCTG CTCCATACAG CCAGAACACA 
    CCTGTCGCTC CGCCCACATC CCTGCCCAAA CTCACCAACA TCGAGGTGAA CTCTCTGCCA 
    GATCCTCACA GCTCTCCCAA AGCCAAACCG GACCTGTCTG CTCTGAGAGC TCCGACCCGG 
    GACGAGCCCA TCCAGAGCAG CTACTTGCCC CCCAAGTCCA CCATCAATGG CATGGATGCT 
    CCTCCCAAAA CACTCGGCGT GCCGACCAGC TACAACCGCT ACGTCCCCAA GCCTTACACC 
    AGCTCGGCGC GACCCTTCGA GCGCAAGTTC GAGAGTCCGA AATTCAACCA CAACCTGCTT 
    CCCAACGACA CGCAGTCCAA ACCCAGTGTC AACAACAACC TGAAGCCTCA GATCTCCCCA 
    CAACCCCAGG ACACGGACAG CGGCGTCGAC ACCTTCACTC GCACCATGGA CAACAGGCCA 
    AAATATCAGC ACAACAACAT CAACGCCATT CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA 
    CTGGATGATG ATGAAGAAGA TGAGGGACAC ACAGTTGTGG CCACGGCGCG GGGCATCTTT 
    AACTGTAATG GCGGGGTGTT GAGCTCTATA GAGACGGGCG TCAGCATCAT TATCCCACAG 
    GGAGCCATTC CTGATAGTGT GGAGCAGGAG ATCTACTTCA AAGTGTGCCG GGACAACAGC 
    ATCCTGCCTC CTCTGGATAA GGAGAAAGGT GAAACTCTGC TGAGTCCGCT GGTGATGTGT 
    GGTCCTCACG GCCTGAAGTT CCTGAAGCCT GTGGAGCTGC GCTTGCCACA CTGTGCGTCT 
    ATGACCCCTG ATGGTTGGTC TTTTGCTCTA AAATCCTCCG ACTCCTCGTC GGGTGACCCC 
    AAATGCTGGC AAAACAAGTG TCTACCAGGA GACCCCAATT ACCTGGTGGG CGCAAACTGT 
    GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_021470732.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326407.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA.
    Price & Availability↑
    CloneID ODa44624
    Clone ID Related Accession (Same CDS sequence) XM_021470732.1
    Accession Version XM_021470732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6072bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
    ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
    TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
    AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
    GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
    AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
    GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
    GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
    GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
    CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
    ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
    GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
    TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
    GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
    CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
    TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
    TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
    TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
    GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
    CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
    CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
    CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
    AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
    ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
    ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
    GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
    TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
    GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
    AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
    GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
    ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
    TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
    GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
    CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
    CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
    GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
    ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
    GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
    GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
    GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
    GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
    GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
    TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
    CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
    CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
    AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
    GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
    TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
    GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
    AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
    ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
    AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
    GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
    AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
    AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
    CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
    CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
    ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
    CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
    AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
    GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
    TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
    TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
    GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
    AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
    GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
    GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
    AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
    GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
    AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG ACCCCAAATG CTGGCAAAAC 
    AAGTGTCTAC CAGGAGACCC CAATTACCTG GTGGGCGCAA ACTGTGTGTC TGTGCTGATC 
    GACCACTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326407.1
    CDS110..6181
    Translation

    Target ORF information:

    RefSeq Version XM_021470732.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021470732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCATG TATGGCGTCA GGAAGTTCTC AGAAGGTGAC ATCAGCAGAA 
    TACGGGCTTC GCCTGGCCAG CCACATCTTC GTGAAGGACA TTTCCCCCGA GAGCCTGGCA 
    GCCCGAGACG GAAACATCCA AGAGGGAGAC GTGGTGCTTA AGATTAATGG CACGGTAACA 
    GAGAATTTGT CCCTGATCGA TGCTAAGAAG CTGATCGAGA GGTCAAAGGG AAAGCTGAAG 
    ATGGTGGTGC AGAGAGACGA GCGGGCGACG CTGCTGAACA TCCCCGATCT GGACGACAGC 
    ATACCTTCTG CTAATGCCTC GGACAGAGAT GATATTTCAG ACATCCACTC CGTGGCCTCT 
    GATCATTCCA ACCGATCCCA CGACCGGAAG AGGAGCAGCC GCTCTCGTTC TCCAGACCGC 
    CGCTCCGAAC CCTCAGACCA CTCCAGACAC TCTCCACCAC AGATCAGCAA CGGCAGGGCC 
    ACAGGGCCGA CGGCTCAGAG AACGCGGCCA GTTCACAGGA GCCGTGATGA AGACAGGATC 
    TCCAAGGCCG GGCCGCTTCC TCTACCTGCT AAGATGGTGG AGGAGATGCC GAAAGAGCAG 
    AGCGCAGCCA GAGAAACAGA GAAACAGCTC CCCCCTCTGC CAGAGCCCAA ACCTGTGTAC 
    GCTCAGCCGG GCCAGCCGGA CGTGGACCTG CCTGTTAGTC CGGCAGATGC TCCTGTGCCC 
    AGCGTCACAC ATGATGACAG CATCTTACGG CCGAGCATGA AGCTGGTGAA GTTCAAGAAG 
    GGCGAGAGCG TGGGCTTGAG GCTCGCTGGG GGGAACGACG TTGGGATATT TGTGGCGGGA 
    GTTCTGGAGG ACAGTCCTGC TGCTAAAGAG GGCCTGGAAG AGGGAGACCA GATTCTCAGG 
    GTAAATAATG TGGATTTTGC AAATATCATC CGAGAGGAGG CAGTGCTGTT CCTGCTGGAT 
    CTGCCCAAAG GAGAGGAGGT CACCATCCTG GCCCAGAAGA AGAAAGACGT GTACCGCCGC 
    ATTGTAGAGT CTGACGTGGG AGACTCGTTC TACATCAGGA CTCATTTTGA ATATGAGAAG 
    GAGTCGCCCT ATGGCCTGAG CTTCAATAAG GGGGAGGTCT TCAGAGTCGT CGACACCCTT 
    TACAATGGCA AACTGGGCTC CTGGCTCGCC ATCCGCATCG GCAAAAACCA CCAGGAGGTG 
    GAGCGGGGCA TCATCCCCAA CAAGAACAGG GCTGAGCAGT TATCAAGTGT CCAGTACACT 
    CTCCCAAAGA CTCCAGGAGG GGATCGCGCC GACTTCTGGA GATTCAGAGG CCTCCGCAGC 
    TCCAAACGAA ACCTGAGGAA GAGCAGAGAA GACCTGTCTG CTCAGCCGGT CCAAACCAAA 
    TTCCCTGCTT ATGAAAGAGT GGTGCTTAGA GAAGCTGGTT TCTTGAGGCC AGTGGTCATT 
    TTCGGACCGA TCGCGGATGT GGCTAGAGAG AAGCTGTCCA GAGAGGAGAC TGACCTCTTC 
    GAACTTGCAA AGAGTGAACC CAGAGATGCG GGCACAGACC AGCGCAGCTC CGGAATCATC 
    CGTCTCCATA CCATTAAACA GATCATTGAC CGAGACAAGC ACGCGGTGCT GGACATCACC 
    CCTAATGCGG TGGACCGTCT GAACTACGCT CAGTGGTACC CCATCGTGGT GTTTCTGAAT 
    CCAGACAGCA AACAGGGCGT CAAGAACATG AGGACCAGGC TCTGTCCCGA GTCCAGGAAG 
    AGCGCCCGAA AGCTCTACGA GCGTGCTCTC AAACTGAGGA AGAACAACCA TCACCTTTTC 
    ACCACCACTA TTAACTTGAA CAACATGAAT GACGGGTGGT ACGGTGCTTT GAAAGAGAGC 
    ATCCAGCAGC AGCAGAACCA GTTGGTCTGG GTCTCAGAGG GCAAGGCCGA CGGCGCTCCT 
    GACGATGACC TTGACATCCA TGACGACCGC CTCTCCTACC TCTCGGCTCC GGGCAGCGAG 
    TACAGCATGT ACAGCACCGA CAGCCGCCAC ACGTCCGACT ATGAGGACAC CGACACGGAG 
    GGTGGTGCCT ACACCGACCA GGAGCTGGAC GAGACCATGA ACGATGAGGT GGGCCTGCCT 
    AGCGAGCCTG CCATCACACG CTCATCTGAG CCTGTGCGAG AGGACCCGCC AGTCATTCAG 
    GACGCAGCTG GATACCCCAG CTACCAGCAT GCGGTACCGC AACCTGAAGC GGTGAGCCGC 
    ATTGACCCGG CCGGGTTTAA GATGGCAGCG CCGCAGCAGC AAGCTGAGGC TGCTCTGGCC 
    TCGCCGCCCC CTCCCTCTGC AGCGGTCCAG CCCACCGCAA CACTAGCGGG TACGAACCCC 
    GAGGAGCCGC CTGGCACCCC TCAGGCCGAC TCCCTTAACA GCCCCGTCCC TGTTCCCGAC 
    CCCCAGCCCG AACCTGAGCT CGCTCAGCCC CCAACACACG ACCCCCACCA GTCGCTTCCT 
    CCTGGCCCAG ATCCAAAGAT GTATAAAAAG GATCTGTACA GCATGGACGA GCCGGTGCGA 
    GTGACCCACG GCGTGAAGCC TCCCCCTCCC CAGCAGCAGC AGCAGCCTTT AGGCCCCCCA 
    ACCTCGCTCT CCTACAGCCA CCAGCCTGTC TACCAGGACC AACAGCCATA CCGCCAGTAC 
    GACCACCCGC CTTATGGCTA TGACGGTGGC GGCTACGCAC AACCAAAGCC TCACAACTAC 
    GACCCGCACC TGCACTACGA CAACCGTGTG CCTCACTATA ACGAGCAGTG GCCCACGTAC 
    GACCAGCAGC AGCAGCAGCA GACTTCCCCC CAGCCGCCAC CTGCGTCCGG CTTCCCTCAG 
    GGCCACCAGC CTCCGCCTCC ACCACCGATG GGATATGAGC CACGCTCCCC ATACGAGGAC 
    GGACCTAACA GGGATTTCAG CCCTCCTCAG TCCCAGTATG ACGGTGTGTC CCCAATGGGC 
    TACGATCACC GGCCGCGGCA CCCCAAACCT GCACCGGTCC GATATGAAGA GCCTCCACCT 
    CCACCTCCTC CGGTGTCCTA CGATGCACGG TCACCTTTCG AACCCGAGCC GCATGCTTTC 
    CCAGGCAACA CACATCGCTC GCCAGACCCA CCGAAACAGT ATTATGGAGA CGCCGCTATG 
    AGGCCCTTGT ACAACCCTGG ACCTCCGAAC AGAGTGTACA AACATGAGCC GGTGAACTCT 
    GAGCCTCCTG TACCGCCTCC CAAACCTGAA TCTGTCCTGT CTCCAGGAGA ACCGCCCCAT 
    TCTGCAGCTT CCAAACCATT GCCGCCTCCT CCCAGAGATG ATGACGATGA TGATGACGAT 
    GATGATGACC CTGCCATGAA GCCCCAGTCT GTGCTCAACA GGGTCAAAAT GTTCGAAAAC 
    AAACGCTCTG TGTCGGTAGA CCGAGCCAAG GACGCACCTG ACGTCACTGG AATTAGGCCC 
    ACAGATCTCC CCAAACCTGC GAGCACCCCT GGACCTGTGC TCAAAGCCAA CTCTCTCAGC 
    AACCTGGAGC AGGACAAACC CTCCTACAGA GCTCCAGAGC CGCAGAAACC TCAGACGCGT 
    GTTGGGGACG ATGTGGTTCG CTCCAACCAC TACGACCCTG ATGATGATGA AGAGTACTAC 
    AGAAAACAGC TCTCATTTTT CGACCGCCGC AGTTTCGACA GTAAGGTCAT GAATCCACCC 
    AGCGCTGGAG TAAACCGCTT CCATGAGCCG CCCAAACCAC CCCAGACACA GATCGCTTAT 
    CCTTTCGCTA GGACGGAGTC TGTGGAGAAG GTGAGTCCTG TGGACAAGAG ATACGAGCCT 
    CTGCCGCCAG TCAACCCTTC ACCTGCTCCA TACAGCCAGA ACACACCTGT CGCTCCGCCC 
    ACATCCCTGC CCAAACTCAC CAACATCGAG GTGAACTCTC TGCCAGATCC TCACAGCTCT 
    CCCAAAGCCA AACCGGACCT GTCTGCTCTG AGAGCTCCGA CCCGGGACGA GCCCATCCAG 
    AGCAGCTACT TGCCCCCCAA GTCCACCATC AATGGCATGG ATGCTCCTCC CAAAACACTC 
    GGCGTGCCGA CCAGCTACAA CCGCTACGTC CCCAAGCCTT ACACCAGCTC GGCGCGACCC 
    TTCGAGCGCA AGTTCGAGAG TCCGAAATTC AACCACAACC TGCTTCCCAA CGACACGCAG 
    TCCAAACCCA GTGTCAACAA CAACCTGAAG CCTCAGATCT CCCCACAACC CCAGGACACG 
    GACAGCGGCG TCGACACCTT CACTCGCACC ATGGACAACA GGCCAAAATA TCAGCACAAC 
    AACATCAACG CCATTCCCAA AGCCGTCCCA GTCAGCCCCA GTGCACTGGA TGATGATGAA 
    GAAGATGAGG GACACACAGT TGTGGCCACG GCGCGGGGCA TCTTTAACTG TAATGGCGGG 
    GTGTTGAGCT CTATAGAGAC GGGCGTCAGC ATCATTATCC CACAGGGAGC CATTCCTGAT 
    AGTGTGGAGC AGGAGATCTA CTTCAAAGTG TGCCGGGACA ACAGCATCCT GCCTCCTCTG 
    GATAAGGAGA AAGGTGAAAC TCTGCTGAGT CCGCTGGTGA TGTGTGGTCC TCACGGCCTG 
    AAGTTCCTGA AGCCTGTGGA GCTGCGCTTG CCACACTGTG ACCCCAAATG CTGGCAAAAC 
    AAGTGTCTAC CAGGAGACCC CAATTACCTG GTGGGCGCAA ACTGTGTGTC TGTGCTGATC 
    GACCACTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_009298135.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296410.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA.
    Price & Availability↑
    CloneID ODa44625
    Clone ID Related Accession (Same CDS sequence) XM_009298135.3 , XM_009298135.2
    Accession Version XM_009298135.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
    CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
    GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
    GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
    AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
    AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
    GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
    GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
    GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
    GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
    AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
    TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
    AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
    GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
    AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
    GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
    GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
    CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
    CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
    CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
    GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
    GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
    AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
    CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
    CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
    GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
    CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
    CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
    AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
    AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
    GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
    CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
    CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
    ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
    CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
    CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
    TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
    GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
    CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
    CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
    AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
    CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
    TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
    TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
    GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
    CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
    TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
    AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
    GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
    GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
    CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
    CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
    TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
    TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
    TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
    TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
    ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
    CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
    AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
    AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
    AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
    AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
    TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
    AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
    GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
    ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
    TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
    ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
    GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
    TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296410.1
    CDS110..6163
    Translation

    Target ORF information:

    RefSeq Version XM_009298135.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298135.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
    CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
    GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
    GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
    AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
    AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
    GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
    GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
    GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
    GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
    AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
    TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
    AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
    GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
    AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
    GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
    GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
    CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
    CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
    CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
    GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
    GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
    AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
    CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
    CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
    GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
    CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
    CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
    AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
    AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
    GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
    CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
    CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
    ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
    CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
    CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
    TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
    GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
    CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
    CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
    AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
    CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
    TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
    TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
    GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
    CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
    TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
    AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
    GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
    GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
    CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
    CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
    TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
    TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
    TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
    TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
    ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
    CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
    AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
    AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
    AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
    AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
    TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
    AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
    GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
    ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
    TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
    ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
    GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
    TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_009298135.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296410.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID ODa44625
    Clone ID Related Accession (Same CDS sequence) XM_009298135.3 , XM_009298135.2
    Accession Version XM_009298135.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
    CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
    GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
    GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
    AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
    AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
    GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
    GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
    GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
    GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
    AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
    TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
    AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
    GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
    AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
    GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
    GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
    CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
    CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
    CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
    GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
    GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
    AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
    CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
    CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
    GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
    CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
    CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
    AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
    AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
    GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
    CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
    CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
    ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
    CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
    CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
    TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
    GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
    CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
    CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
    AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
    CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
    TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
    TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
    GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
    CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
    TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
    AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
    GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
    GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
    CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
    CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
    TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
    TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
    TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
    TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
    ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
    CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
    AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
    AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
    AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
    AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
    TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
    AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
    GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
    ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
    TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
    ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
    GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
    TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296410.1
    CDS115..6168
    Translation

    Target ORF information:

    RefSeq Version XM_009298135.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009298135.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGAATA CGGGCTTCGC CTGGCCAGCC ACATCTTCGT GAAGGACATT 
    TCCCCCGAGA GCCTGGCAGC CCGAGACGGA AACATCCAAG AGGGAGACGT GGTGCTTAAG 
    ATTAATGGCA CGGTAACAGA GAATTTGTCC CTGATCGATG CTAAGAAGCT GATCGAGAGG 
    TCAAAGGGAA AGCTGAAGAT GGTGGTGCAG AGAGACGAGC GGGCGACGCT GCTGAACATC 
    CCCGATCTGG ACGACAGCAT ACCTTCTGCT AATGCCTCGG ACAGAGATGA TATTTCAGAC 
    ATCCACTCCG TGGCCTCTGA TCATTCCAAC CGATCCCACG ACCGGAAGAG GAGCAGCCGC 
    TCTCGTTCTC CAGACCGCCG CTCCGAACCC TCAGACCACT CCAGACACTC TCCACCACAG 
    ATCAGCAACG GCAGTCACAG GAGCCGTGAT GAAGACAGGA TCTCCAAGGC CGGGCCGCTT 
    CCTCTACCTG CTAAGATGGT GGAGGAGATG CCGAAAGAGC AGAGCGCAGC CAGAGAAACA 
    GAGAAACAGC TCCCCCCTCT GCCAGAGCCC AAACCTGTGT ACGCTCAGCC GGGCCAGCCG 
    GACGTGGACC TGCCTGTTAG TCCGGCAGAT GCTCCTGTGC CCAGCGTCAC ACATGATGAC 
    AGCATCTTAC GGCCGAGCAT GAAGCTGGTG AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG 
    AGGCTCGCTG GGGGGAACGA CGTTGGGATA TTTGTGGCGG GAGTTCTGGA GGACAGTCCT 
    GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC CAGATTCTCA GGGTAAATAA TGTGGATTTT 
    GCAAATATCA TCCGAGAGGA GGCAGTGCTG TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG 
    GTCACCATCC TGGCCCAGAA GAAGAAAGAC GTGTACCGCC GCATTGTAGA GTCTGACGTG 
    GGAGACTCGT TCTACATCAG GACTCATTTT GAATATGAGA AGGAGTCGCC CTATGGCCTG 
    AGCTTCAATA AGGGGGAGGT CTTCAGAGTC GTCGACACCC TTTACAATGG CAAACTGGGC 
    TCCTGGCTCG CCATCCGCAT CGGCAAAAAC CACCAGGAGG TGGAGCGGGG CATCATCCCC 
    AACAAGAACA GGGCTGAGCA GTTATCAAGT GTCCAGTACA CTCTCCCAAA GACTCCAGGA 
    GGGGATCGCG CCGACTTCTG GAGATTCAGA GGCCTCCGCA GCTCCAAACG AAACCTGAGG 
    AAGAGCAGAG AAGACCTGTC TGCTCAGCCG GTCCAAACCA AATTCCCTGC TTATGAAAGA 
    GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG CCAGTGGTCA TTTTCGGACC GATCGCGGAT 
    GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG ACTGACCTCT TCGAACTTGC AAAGAGTGAA 
    CCCAGAGATG CGGGCACAGA CCAGCGCAGC TCCGGAATCA TCCGTCTCCA TACCATTAAA 
    CAGATCATTG ACCGAGACAA GCACGCGGTG CTGGACATCA CCCCTAATGC GGTGGACCGT 
    CTGAACTACG CTCAGTGGTA CCCCATCGTG GTGTTTCTGA ATCCAGACAG CAAACAGGGC 
    GTCAAGAACA TGAGGACCAG GCTCTGTCCC GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC 
    GAGCGTGCTC TCAAACTGAG GAAGAACAAC CATCACCTTT TCACCACCAC TATTAACTTG 
    AACAACATGA ATGACGGGTG GTACGGTGCT TTGAAAGAGA GCATCCAGCA GCAGCAGAAC 
    CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC GACGGCGCTC CTGACGATGA CCTTGACATC 
    CATGACGACC GCCTCTCCTA CCTCTCGGCT CCGGGCAGCG AGTACAGCAT GTACAGCACC 
    GACAGCCGCC ACACGTCCGA CTATGAGGAC ACCGACACGG AGGGTGGTGC CTACACCGAC 
    CAGGAGCTGG ACGAGACCAT GAACGATGAG GTGGGCCTGC CTAGCGAGCC TGCCATCACA 
    CGCTCATCTG AGCCTGTGCG AGAGGACCCG CCAGTCATTC AGGACGCAGC TGGATACCCC 
    AGCTACCAGC ATGCGGTACC GCAACCTGAA GCGGTGAGCC GCATTGACCC GGCCGGGTTT 
    AAGATGGCAG CGCCGCAGCA GCAAGCTGAG GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT 
    GCAGCGGTCC AGCCCACCGC AACACTAGCG GGTACGAACC CCGAGGAGCC GCCTGGCACC 
    CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC CCTGTTCCCG ACCCCCAGCC CGAACCTGAG 
    CTCGCTCAGC CCCCAACACA CGACCCCCAC CAGTCGCTTC CTCCTGGCCC AGATCCAAAG 
    ATGTATAAAA AGGATCTGTA CAGCATGGAC GAGCCGGTGC GAGTGACCCA CGGCGTGAAG 
    CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT TTAGGCCCCC CAACCTCGCT CTCCTACAGC 
    CACCAGCCTG TCTACCAGGA CCAACAGCCA TACCGCCAGT ACGACCACCC GCCTTATGGC 
    TATGACGGTG GCGGCTACGC ACAACCAAAG CCTCACAACT ACGACCCGCA CCTGCACTAC 
    GACAACCGTG TGCCTCACTA TAACGAGCAG TGGCCCACGT ACGACCAGCA GCAGCAGCAG 
    CAGACTTCCC CCCAGCCGCC ACCTGCGTCC GGCTTCCCTC AGGGCCACCA GCCTCCGCCT 
    CCACCACCGA TGGGATATGA GCCACGCTCC CCATACGAGG ACGGACCTAA CAGGGATTTC 
    AGCCCTCCTC AGTCCCAGTA TGACGGTGTG TCCCCAATGG GCTACGATCA CCGGCCGCGG 
    CACCCCAAAC CTGCACCGGT CCGATATGAA GAGCCTCCAC CTCCACCTCC TCCGGTGTCC 
    TACGATGCAC GGTCACCTTT CGAACCCGAG CCGCATGCTT TCCCAGGCAA CACACATCGC 
    TCGCCAGACC CACCGAAACA GTATTATGGA GACGCCGCTA TGAGGCCCTT GTACAACCCT 
    GGACCTCCGA ACAGAGTGTA CAAACATGAG CCGGTGAACT CTGAGCCTCC TGTACCGCCT 
    CCCAAACCTG AATCTGTCCT GTCTCCAGGA GAACCGCCCC ATTCTGCAGC TTCCAAACCA 
    TTGCCGCCTC CTCCCAGAGA TGATGACGAT GATGATGACG ATGATGATGA CCCTGCCATG 
    AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA ATGTTCGAAA ACAAACGCTC TGTGTCGGTA 
    GACCGAGCCA AGGACGCACC TGACGTCACT GGAATTAGGC CCACAGATCT CCCCAAACCT 
    GCGAGCACCC CTGGACCTGT GCTCAAAGCC AACTCTCTCA GCAACCTGGA GCAGGACAAA 
    CCCTCCTACA GAGCTCCAGA GCCGCAGAAA CCTCAGACGC GTGTTGGGGA CGATGTGGTT 
    CGCTCCAACC ACTACGACCC TGATGATGAT GAAGAGTACT ACAGAAAACA GCTCTCATTT 
    TTCGACCGCC GCAGTTTCGA CAGTAAGGTC ATGAATCCAC CCAGCGCTGG AGTAAACCGC 
    TTCCATGAGC CGCCCAAACC ACCCCAGACA CAGATCGCTT ATCCTTTCGC TAGGACGGAG 
    TCTGTGGAGA AGGTGAGTCC TGTGGACAAG AGATACGAGC CTCTGCCGCC AGTCAACCCT 
    TCACCTGCTC CATACAGCCA GAACACACCT GTCGCTCCGC CCACATCCCT GCCCAAACTC 
    ACCAACATCG AGGTGAACTC TCTGCCAGAT CCTCACAGCT CTCCCAAAGC CAAACCGGAC 
    CTGTCTGCTC TGAGAGCTCC GACCCGGGAC GAGCCCATCC AGAGCAGCTA CTTGCCCCCC 
    AAGTCCACCA TCAATGGCAT GGATGCTCCT CCCAAAACAC TCGGCGTGCC GACCAGCTAC 
    AACCGCTACG TCCCCAAGCC TTACACCAGC TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG 
    AGTCCGAAAT TCAACCACAA CCTGCTTCCC AACGACACGC AGTCCAAACC CAGTGTCAAC 
    AACAACCTGA AGCCTCAGAT CTCCCCACAA CCCCAGGACA CGGACAGCGG CGTCGACACC 
    TTCACTCGCA CCATGGACAA CAGGCCAAAA TATCAGCACA ACAACATCAA CGCCATTCCC 
    AAAGCCGTCC CAGTCAGCCC CAGTGCACTG GATGATGATG AAGAAGATGA GGGACACACA 
    GTTGTGGCCA CGGCGCGGGG CATCTTTAAC TGTAATGGCG GGGTGTTGAG CTCTATAGAG 
    ACGGGCGTCA GCATCATTAT CCCACAGGGA GCCATTCCTG ATAGTGTGGA GCAGGAGATC 
    TACTTCAAAG TGTGCCGGGA CAACAGCATC CTGCCTCCTC TGGATAAGGA GAAAGGTGAA 
    ACTCTGCTGA GTCCGCTGGT GATGTGTGGT CCTCACGGCC TGAAGTTCCT GAAGCCTGTG 
    GAGCTGCGCT TGCCACACTG TGCGTCTATG ACCCCTGATG GTTGGTCTTT TGCTCTAAAA 
    TCCTCCGACT CCTCGTCGGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354168.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209657.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa57879
    Clone ID Related Accession (Same CDS sequence) XM_017354168.1
    Accession Version XM_017354168.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6084bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGACATCCAC 
    TCCGTGGCCT CTGATCATTC CAACCGATCC CACGACCGGA AGAGGAGCAG CCGCTCTCGT 
    TCTCCAGACC GCCGCTCCGA ACCCTCAGAC CACTCCAGAC ACTCTCCACC ACAGATCAGC 
    AACGGCAGGG CCACAGGGCC GACGGCTCAG AGAACGCGGC CAGTTCACAG GAGCCGTGAT 
    GAAGACAGGA TCTCCAAGGC CGGGCCGCTT CCTCTACCTG CTAAGATGGT GGAGGAGATG 
    CCGAAAGAGC AGAGCGCAGC CAGAGAAACA GAGAAACAGC TCCCCCCTCT GCCAGAGCCC 
    AAACCTGTGT ACGCTCAGCC GGGCCAGCCG GACGTGGACC TGCCTGTTAG TCCGGCAGAT 
    GCTCCTGTGC CCAGCGTCAC ACATGATGAC AGCATCTTAC GGCCGAGCAT GAAGCTGGTG 
    AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG AGGCTCGCTG GGGGGAACGA CGTTGGGATA 
    TTTGTGGCGG GAGTTCTGGA GGACAGTCCT GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC 
    CAGATTCTCA GGGTAAATAA TGTGGATTTT GCAAATATCA TCCGAGAGGA GGCAGTGCTG 
    TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG GTCACCATCC TGGCCCAGAA GAAGAAAGAC 
    GTGTACCGCC GCATTGTAGA GTCTGACGTG GGAGACTCGT TCTACATCAG GACTCATTTT 
    GAATATGAGA AGGAGTCGCC CTATGGCCTG AGCTTCAATA AGGGGGAGGT CTTCAGAGTC 
    GTCGACACCC TTTACAATGG CAAACTGGGC TCCTGGCTCG CCATCCGCAT CGGCAAAAAC 
    CACCAGGAGG TGGAGCGGGG CATCATCCCC AACAAGAACA GGGCTGAGCA GTTATCAAGT 
    GTCCAGTACA CTCTCCCAAA GACTCCAGGA GGGGATCGCG CCGACTTCTG GAGATTCAGA 
    GGCCTCCGCA GCTCCAAACG AAACCTGAGG AAGAGCAGAG AAGACCTGTC TGCTCAGCCG 
    GTCCAAACCA AATTCCCTGC TTATGAAAGA GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG 
    CCAGTGGTCA TTTTCGGACC GATCGCGGAT GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG 
    ACTGACCTCT TCGAACTTGC AAAGAGTGAA CCCAGAGATG CGGGCACAGA CCAGCGCAGC 
    TCCGGAATCA TCCGTCTCCA TACCATTAAA CAGATCATTG ACCGAGACAA GCACGCGGTG 
    CTGGACATCA CCCCTAATGC GGTGGACCGT CTGAACTACG CTCAGTGGTA CCCCATCGTG 
    GTGTTTCTGA ATCCAGACAG CAAACAGGGC GTCAAGAACA TGAGGACCAG GCTCTGTCCC 
    GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC GAGCGTGCTC TCAAACTGAG GAAGAACAAC 
    CATCACCTTT TCACCACCAC TATTAACTTG AACAACATGA ATGACGGGTG GTACGGTGCT 
    TTGAAAGAGA GCATCCAGCA GCAGCAGAAC CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC 
    GACGGCGCTC CTGACGATGA CCTTGACATC CATGACGACC GCCTCTCCTA CCTCTCGGCT 
    CCGGGCAGCG AGTACAGCAT GTACAGCACC GACAGCCGCC ACACGTCCGA CTATGAGGAC 
    ACCGACACGG AGGGTGGTGC CTACACCGAC CAGGAGCTGG ACGAGACCAT GAACGATGAG 
    GTGGGCCTGC CTAGCGAGCC TGCCATCACA CGCTCATCTG AGCCTGTGCG AGAGGACCCG 
    CCAGTCATTC AGGACGCAGC TGGATACCCC AGCTACCAGC ATGCGGTACC GCAACCTGAA 
    GCGGTGAGCC GCATTGACCC GGCCGGGTTT AAGATGGCAG CGCCGCAGCA GCAAGCTGAG 
    GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT GCAGCGGTCC AGCCCACCGC AACACTAGCG 
    GGTACGAACC CCGAGGAGCC GCCTGGCACC CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC 
    CCTGTTCCCG ACCCCCAGCC CGAACCTGAG CTCGCTCAGC CCCCAACACA CGACCCCCAC 
    CAGTCGCTTC CTCCTGGCCC AGATCCAAAG ATGTATAAAA AGGATCTGTA CAGCATGGAC 
    GAGCCGGTGC GAGTGACCCA CGGCGTGAAG CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT 
    TTAGGCCCCC CAACCTCGCT CTCCTACAGC CACCAGCCTG TCTACCAGGA CCAACAGCCA 
    TACCGCCAGT ACGACCACCC GCCTTATGGC TATGACGGTG GCGGCTACGC ACAACCAAAG 
    CCTCACAACT ACGACCCGCA CCTGCACTAC GACAACCGTG TGCCTCACTA TAACGAGCAG 
    TGGCCCACGT ACGACCAGCA GCAGCAGCAG CAGACTTCCC CCCAGCCGCC ACCTGCGTCC 
    GGCTTCCCTC AGGGCCACCA GCCTCCGCCT CCACCACCGA TGGGATATGA GCCACGCTCC 
    CCATACGAGG ACGGACCTAA CAGGGATTTC AGCCCTCCTC AGTCCCAGTA TGACGGTGTG 
    TCCCCAATGG GCTACGATCA CCGGCCGCGG CACCCCAAAC CTGCACCGGT CCGATATGAA 
    GAGCCTCCAC CTCCACCTCC TCCGGTGTCC TACGATGCAC GGTCACCTTT CGAACCCGAG 
    CCGCATGCTT TCCCAGGCAA CACACATCGC TCGCCAGACC CACCGAAACA GTATTATGGA 
    GACGCCGCTA TGAGGCCCTT GTACAACCCT GGACCTCCGA ACAGAGTGTA CAAACATGAG 
    CCGGTGAACT CTGAGCCTCC TGTACCGCCT CCCAAACCTG AATCTGTCCT GTCTCCAGGA 
    GAACCGCCCC ATTCTGCAGC TTCCAAACCA TTGCCGCCTC CTCCCAGAGA TGATGACGAT 
    GATGATGACG ATGATGATGA CCCTGCCATG AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA 
    ATGTTCGAAA ACAAACGCTC TGTGTCGGTA GACCGAGCCA AGGACGCACC TGACGTCACT 
    GGAATTAGGC CCACAGATCT CCCCAAACCT GCGAGCACCC CTGGACCTGT GCTCAAAGCC 
    AACTCTCTCA GCAACCTGGA GCAGGACAAA CCCTCCTACA GAGCTCCAGA GCCGCAGAAA 
    CCTCAGACGC GTGTTGGGGA CGATGTGGTT CGCTCCAACC ACTACGACCC TGATGATGAT 
    GAAGAGTACT ACAGAAAACA GCTCTCATTT TTCGACCGCC GCAGTTTCGA CAGTAAGGTC 
    ATGAATCCAC CCAGCGCTGG AGTAAACCGC TTCCATGAGC CGCCCAAACC ACCCCAGACA 
    CAGATCGCTT ATCCTTTCGC TAGGACGGAG TCTGTGGAGA AGGTGAGTCC TGTGGACAAG 
    AGATACGAGC CTCTGCCGCC AGTCAACCCT TCACCTGCTC CATACAGCCA GAACACACCT 
    GTCGCTCCGC CCACATCCCT GCCCAAACTC ACCAACATCG AGGTGAACTC TCTGCCAGAT 
    CCTCACAGCT CTCCCAAAGC CAAACCGGAC CTGTCTGCTC TGAGAGCTCC GACCCGGGAC 
    GAGCCCATCC AGAGCAGCTA CTTGCCCCCC AAGTCCACCA TCAATGGCAT GGATGCTCCT 
    CCCAAAACAC TCGGCGTGCC GACCAGCTAC AACCGCTACG TCCCCAAGCC TTACACCAGC 
    TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG AGTCCGAAAT TCAACCACAA CCTGCTTCCC 
    AACGACACGC AGTCCAAACC CAGTGTCAAC AACAACCTGA AGCCTCAGAT CTCCCCACAA 
    CCCCAGGACA CGGACAGCGG CGTCGACACC TTCACTCGCA CCATGGACAA CAGGCCAAAA 
    TATCAGCACA ACAACATCAA CGCCATTCCC AAAGCCGTCC CAGTCAGCCC CAGTGCACTG 
    GATGATGATG AAGAAGATGA GGGACACACA GTTGTGGCCA CGGCGCGGGG CATCTTTAAC 
    TGTAATGGCG GGGTGTTGAG CTCTATAGAG ACGGGCGTCA GCATCATTAT CCCACAGGGA 
    GCCATTCCTG ATAGTGTGGA GCAGGAGATC TACTTCAAAG TGTGCCGGGA CAACAGCATC 
    CTGCCTCCTC TGGATAAGGA GAAAGGTGAA ACTCTGCTGA GTCCGCTGGT GATGTGTGGT 
    CCTCACGGCC TGAAGTTCCT GAAGCCTGTG GAGCTGCGCT TGCCACACTG TGCGTCTATG 
    ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGACCCCAAA 
    TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC CCCAATTACC TGGTGGGCGC AAACTGTGTG 
    TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209657.1
    CDS115..6198
    Translation

    Target ORF information:

    RefSeq Version XM_017354168.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354168.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGACATCCAC 
    TCCGTGGCCT CTGATCATTC CAACCGATCC CACGACCGGA AGAGGAGCAG CCGCTCTCGT 
    TCTCCAGACC GCCGCTCCGA ACCCTCAGAC CACTCCAGAC ACTCTCCACC ACAGATCAGC 
    AACGGCAGGG CCACAGGGCC GACGGCTCAG AGAACGCGGC CAGTTCACAG GAGCCGTGAT 
    GAAGACAGGA TCTCCAAGGC CGGGCCGCTT CCTCTACCTG CTAAGATGGT GGAGGAGATG 
    CCGAAAGAGC AGAGCGCAGC CAGAGAAACA GAGAAACAGC TCCCCCCTCT GCCAGAGCCC 
    AAACCTGTGT ACGCTCAGCC GGGCCAGCCG GACGTGGACC TGCCTGTTAG TCCGGCAGAT 
    GCTCCTGTGC CCAGCGTCAC ACATGATGAC AGCATCTTAC GGCCGAGCAT GAAGCTGGTG 
    AAGTTCAAGA AGGGCGAGAG CGTGGGCTTG AGGCTCGCTG GGGGGAACGA CGTTGGGATA 
    TTTGTGGCGG GAGTTCTGGA GGACAGTCCT GCTGCTAAAG AGGGCCTGGA AGAGGGAGAC 
    CAGATTCTCA GGGTAAATAA TGTGGATTTT GCAAATATCA TCCGAGAGGA GGCAGTGCTG 
    TTCCTGCTGG ATCTGCCCAA AGGAGAGGAG GTCACCATCC TGGCCCAGAA GAAGAAAGAC 
    GTGTACCGCC GCATTGTAGA GTCTGACGTG GGAGACTCGT TCTACATCAG GACTCATTTT 
    GAATATGAGA AGGAGTCGCC CTATGGCCTG AGCTTCAATA AGGGGGAGGT CTTCAGAGTC 
    GTCGACACCC TTTACAATGG CAAACTGGGC TCCTGGCTCG CCATCCGCAT CGGCAAAAAC 
    CACCAGGAGG TGGAGCGGGG CATCATCCCC AACAAGAACA GGGCTGAGCA GTTATCAAGT 
    GTCCAGTACA CTCTCCCAAA GACTCCAGGA GGGGATCGCG CCGACTTCTG GAGATTCAGA 
    GGCCTCCGCA GCTCCAAACG AAACCTGAGG AAGAGCAGAG AAGACCTGTC TGCTCAGCCG 
    GTCCAAACCA AATTCCCTGC TTATGAAAGA GTGGTGCTTA GAGAAGCTGG TTTCTTGAGG 
    CCAGTGGTCA TTTTCGGACC GATCGCGGAT GTGGCTAGAG AGAAGCTGTC CAGAGAGGAG 
    ACTGACCTCT TCGAACTTGC AAAGAGTGAA CCCAGAGATG CGGGCACAGA CCAGCGCAGC 
    TCCGGAATCA TCCGTCTCCA TACCATTAAA CAGATCATTG ACCGAGACAA GCACGCGGTG 
    CTGGACATCA CCCCTAATGC GGTGGACCGT CTGAACTACG CTCAGTGGTA CCCCATCGTG 
    GTGTTTCTGA ATCCAGACAG CAAACAGGGC GTCAAGAACA TGAGGACCAG GCTCTGTCCC 
    GAGTCCAGGA AGAGCGCCCG AAAGCTCTAC GAGCGTGCTC TCAAACTGAG GAAGAACAAC 
    CATCACCTTT TCACCACCAC TATTAACTTG AACAACATGA ATGACGGGTG GTACGGTGCT 
    TTGAAAGAGA GCATCCAGCA GCAGCAGAAC CAGTTGGTCT GGGTCTCAGA GGGCAAGGCC 
    GACGGCGCTC CTGACGATGA CCTTGACATC CATGACGACC GCCTCTCCTA CCTCTCGGCT 
    CCGGGCAGCG AGTACAGCAT GTACAGCACC GACAGCCGCC ACACGTCCGA CTATGAGGAC 
    ACCGACACGG AGGGTGGTGC CTACACCGAC CAGGAGCTGG ACGAGACCAT GAACGATGAG 
    GTGGGCCTGC CTAGCGAGCC TGCCATCACA CGCTCATCTG AGCCTGTGCG AGAGGACCCG 
    CCAGTCATTC AGGACGCAGC TGGATACCCC AGCTACCAGC ATGCGGTACC GCAACCTGAA 
    GCGGTGAGCC GCATTGACCC GGCCGGGTTT AAGATGGCAG CGCCGCAGCA GCAAGCTGAG 
    GCTGCTCTGG CCTCGCCGCC CCCTCCCTCT GCAGCGGTCC AGCCCACCGC AACACTAGCG 
    GGTACGAACC CCGAGGAGCC GCCTGGCACC CCTCAGGCCG ACTCCCTTAA CAGCCCCGTC 
    CCTGTTCCCG ACCCCCAGCC CGAACCTGAG CTCGCTCAGC CCCCAACACA CGACCCCCAC 
    CAGTCGCTTC CTCCTGGCCC AGATCCAAAG ATGTATAAAA AGGATCTGTA CAGCATGGAC 
    GAGCCGGTGC GAGTGACCCA CGGCGTGAAG CCTCCCCCTC CCCAGCAGCA GCAGCAGCCT 
    TTAGGCCCCC CAACCTCGCT CTCCTACAGC CACCAGCCTG TCTACCAGGA CCAACAGCCA 
    TACCGCCAGT ACGACCACCC GCCTTATGGC TATGACGGTG GCGGCTACGC ACAACCAAAG 
    CCTCACAACT ACGACCCGCA CCTGCACTAC GACAACCGTG TGCCTCACTA TAACGAGCAG 
    TGGCCCACGT ACGACCAGCA GCAGCAGCAG CAGACTTCCC CCCAGCCGCC ACCTGCGTCC 
    GGCTTCCCTC AGGGCCACCA GCCTCCGCCT CCACCACCGA TGGGATATGA GCCACGCTCC 
    CCATACGAGG ACGGACCTAA CAGGGATTTC AGCCCTCCTC AGTCCCAGTA TGACGGTGTG 
    TCCCCAATGG GCTACGATCA CCGGCCGCGG CACCCCAAAC CTGCACCGGT CCGATATGAA 
    GAGCCTCCAC CTCCACCTCC TCCGGTGTCC TACGATGCAC GGTCACCTTT CGAACCCGAG 
    CCGCATGCTT TCCCAGGCAA CACACATCGC TCGCCAGACC CACCGAAACA GTATTATGGA 
    GACGCCGCTA TGAGGCCCTT GTACAACCCT GGACCTCCGA ACAGAGTGTA CAAACATGAG 
    CCGGTGAACT CTGAGCCTCC TGTACCGCCT CCCAAACCTG AATCTGTCCT GTCTCCAGGA 
    GAACCGCCCC ATTCTGCAGC TTCCAAACCA TTGCCGCCTC CTCCCAGAGA TGATGACGAT 
    GATGATGACG ATGATGATGA CCCTGCCATG AAGCCCCAGT CTGTGCTCAA CAGGGTCAAA 
    ATGTTCGAAA ACAAACGCTC TGTGTCGGTA GACCGAGCCA AGGACGCACC TGACGTCACT 
    GGAATTAGGC CCACAGATCT CCCCAAACCT GCGAGCACCC CTGGACCTGT GCTCAAAGCC 
    AACTCTCTCA GCAACCTGGA GCAGGACAAA CCCTCCTACA GAGCTCCAGA GCCGCAGAAA 
    CCTCAGACGC GTGTTGGGGA CGATGTGGTT CGCTCCAACC ACTACGACCC TGATGATGAT 
    GAAGAGTACT ACAGAAAACA GCTCTCATTT TTCGACCGCC GCAGTTTCGA CAGTAAGGTC 
    ATGAATCCAC CCAGCGCTGG AGTAAACCGC TTCCATGAGC CGCCCAAACC ACCCCAGACA 
    CAGATCGCTT ATCCTTTCGC TAGGACGGAG TCTGTGGAGA AGGTGAGTCC TGTGGACAAG 
    AGATACGAGC CTCTGCCGCC AGTCAACCCT TCACCTGCTC CATACAGCCA GAACACACCT 
    GTCGCTCCGC CCACATCCCT GCCCAAACTC ACCAACATCG AGGTGAACTC TCTGCCAGAT 
    CCTCACAGCT CTCCCAAAGC CAAACCGGAC CTGTCTGCTC TGAGAGCTCC GACCCGGGAC 
    GAGCCCATCC AGAGCAGCTA CTTGCCCCCC AAGTCCACCA TCAATGGCAT GGATGCTCCT 
    CCCAAAACAC TCGGCGTGCC GACCAGCTAC AACCGCTACG TCCCCAAGCC TTACACCAGC 
    TCGGCGCGAC CCTTCGAGCG CAAGTTCGAG AGTCCGAAAT TCAACCACAA CCTGCTTCCC 
    AACGACACGC AGTCCAAACC CAGTGTCAAC AACAACCTGA AGCCTCAGAT CTCCCCACAA 
    CCCCAGGACA CGGACAGCGG CGTCGACACC TTCACTCGCA CCATGGACAA CAGGCCAAAA 
    TATCAGCACA ACAACATCAA CGCCATTCCC AAAGCCGTCC CAGTCAGCCC CAGTGCACTG 
    GATGATGATG AAGAAGATGA GGGACACACA GTTGTGGCCA CGGCGCGGGG CATCTTTAAC 
    TGTAATGGCG GGGTGTTGAG CTCTATAGAG ACGGGCGTCA GCATCATTAT CCCACAGGGA 
    GCCATTCCTG ATAGTGTGGA GCAGGAGATC TACTTCAAAG TGTGCCGGGA CAACAGCATC 
    CTGCCTCCTC TGGATAAGGA GAAAGGTGAA ACTCTGCTGA GTCCGCTGGT GATGTGTGGT 
    CCTCACGGCC TGAAGTTCCT GAAGCCTGTG GAGCTGCGCT TGCCACACTG TGCGTCTATG 
    ACCCCTGATG GTTGGTCTTT TGCTCTAAAA TCCTCCGACT CCTCGTCGGG TGACCCCAAA 
    TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC CCCAATTACC TGGTGGGCGC AAACTGTGTG 
    TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354169.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209658.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa57880
    Clone ID Related Accession (Same CDS sequence) XM_017354169.1
    Accession Version XM_017354169.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
    ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
    AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
    GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGATCCTCAC 
    AGCTCTCCCA AAGCCAAACC GGACCTGTCT GCTCTGAGAG CTCCGACCCG GGACGAGCCC 
    ATCCAGAGCA GCTACTTGCC CCCCAAGTCC ACCATCAATG GCATGGATGC TCCTCCCAAA 
    ACACTCGGCG TGCCGACCAG CTACAACCGC TACGTCCCCA AGCCTTACAC CAGCTCGGCG 
    CGACCCTTCG AGCGCAAGTT CGAGAGTCCG AAATTCAACC ACAACCTGCT TCCCAACGAC 
    ACGCAGTCCA AACCCAGTGT CAACAACAAC CTGAAGCCTC AGATCTCCCC ACAACCCCAG 
    GACACGGACA GCGGCGTCGA CACCTTCACT CGCACCATGG ACAACAGGCC AAAATATCAG 
    CACAACAACA TCAACGCCAT TCCCAAAGCC GTCCCAGTCA GCCCCAGTGC ACTGGATGAT 
    GATGAAGAAG ATGAGGGACA CACAGTTGTG GCCACGGCGC GGGGCATCTT TAACTGTAAT 
    GGCGGGGTGT TGAGCTCTAT AGAGACGGGC GTCAGCATCA TTATCCCACA GGGAGCCATT 
    CCTGATAGTG TGGAGCAGGA GATCTACTTC AAAGTGTGCC GGGACAACAG CATCCTGCCT 
    CCTCTGGATA AGGAGAAAGG TGAAACTCTG CTGAGTCCGC TGGTGATGTG TGGTCCTCAC 
    GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG CGCTTGCCAC ACTGTGCGTC TATGACCCCT 
    GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGACCC CAAATGCTGG 
    CAAAACAAGT GTCTACCAGG AGACCCCAAT TACCTGGTGG GCGCAAACTG TGTGTCTGTG 
    CTGATCGACC ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209658.1
    CDS115..6192
    Translation

    Target ORF information:

    RefSeq Version XM_017354169.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
    ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
    AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
    GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGATCCTCAC 
    AGCTCTCCCA AAGCCAAACC GGACCTGTCT GCTCTGAGAG CTCCGACCCG GGACGAGCCC 
    ATCCAGAGCA GCTACTTGCC CCCCAAGTCC ACCATCAATG GCATGGATGC TCCTCCCAAA 
    ACACTCGGCG TGCCGACCAG CTACAACCGC TACGTCCCCA AGCCTTACAC CAGCTCGGCG 
    CGACCCTTCG AGCGCAAGTT CGAGAGTCCG AAATTCAACC ACAACCTGCT TCCCAACGAC 
    ACGCAGTCCA AACCCAGTGT CAACAACAAC CTGAAGCCTC AGATCTCCCC ACAACCCCAG 
    GACACGGACA GCGGCGTCGA CACCTTCACT CGCACCATGG ACAACAGGCC AAAATATCAG 
    CACAACAACA TCAACGCCAT TCCCAAAGCC GTCCCAGTCA GCCCCAGTGC ACTGGATGAT 
    GATGAAGAAG ATGAGGGACA CACAGTTGTG GCCACGGCGC GGGGCATCTT TAACTGTAAT 
    GGCGGGGTGT TGAGCTCTAT AGAGACGGGC GTCAGCATCA TTATCCCACA GGGAGCCATT 
    CCTGATAGTG TGGAGCAGGA GATCTACTTC AAAGTGTGCC GGGACAACAG CATCCTGCCT 
    CCTCTGGATA AGGAGAAAGG TGAAACTCTG CTGAGTCCGC TGGTGATGTG TGGTCCTCAC 
    GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG CGCTTGCCAC ACTGTGCGTC TATGACCCCT 
    GATGGTTGGT CTTTTGCTCT AAAATCCTCC GACTCCTCGT CGGGTGACCC CAAATGCTGG 
    CAAAACAAGT GTCTACCAGG AGACCCCAAT TACCTGGTGG GCGCAAACTG TGTGTCTGTG 
    CTGATCGACC ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354170.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209659.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA.
    Price & Availability↑
    CloneID ODa57881
    Clone ID Related Accession (Same CDS sequence) XM_017354170.2 , XM_017354170.1
    Accession Version XM_017354170.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
    CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
    ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
    CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
    GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
    TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
    CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
    TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
    GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
    GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
    CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
    GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
    CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
    GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
    AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
    AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
    GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
    GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
    AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
    CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
    GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
    TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
    TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
    AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
    ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
    ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
    GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
    ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
    CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
    TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
    TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
    ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
    GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
    AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
    AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
    AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
    GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
    TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
    CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
    CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
    TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
    CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
    TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
    CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
    CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
    CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
    CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
    ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
    GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
    CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
    AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
    GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
    TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
    CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
    GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
    CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
    CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209659.1
    CDS110..6166
    Translation

    Target ORF information:

    RefSeq Version XM_017354170.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354170.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
    CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
    ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
    CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
    GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
    TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
    CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
    TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
    GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
    GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
    CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
    GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
    CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
    GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
    AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
    AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
    GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
    GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
    AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
    CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
    GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
    TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
    TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
    AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
    ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
    ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
    GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
    ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
    CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
    TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
    TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
    ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
    GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
    AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
    AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
    AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
    GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
    TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
    CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
    CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
    TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
    CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
    TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
    CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
    CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
    CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
    CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
    ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
    GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
    CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
    AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
    GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
    TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
    CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
    GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
    CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
    CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354170.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209659.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa57881
    Clone ID Related Accession (Same CDS sequence) XM_017354170.2 , XM_017354170.1
    Accession Version XM_017354170.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
    CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
    ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
    CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
    GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
    TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
    CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
    TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
    GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
    GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
    CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
    GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
    CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
    GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
    AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
    AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
    GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
    GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
    AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
    CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
    GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
    TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
    TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
    AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
    ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
    ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
    GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
    ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
    CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
    TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
    TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
    ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
    GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
    AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
    AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
    AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
    GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
    TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
    CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
    CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
    TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
    CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
    TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
    CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
    CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
    CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
    CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
    ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
    GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
    CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
    AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
    GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
    TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
    CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
    GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
    CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
    CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209659.1
    CDS115..6171
    Translation

    Target ORF information:

    RefSeq Version XM_017354170.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354170.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGTCA CAGGAGCCGT GATGAAGACA GGATCTCCAA GGCCGGGCCG 
    CTTCCTCTAC CTGCTAAGAT GGTGGAGGAG ATGCCGAAAG AGCAGAGCGC AGCCAGAGAA 
    ACAGAGAAAC AGCTCCCCCC TCTGCCAGAG CCCAAACCTG TGTACGCTCA GCCGGGCCAG 
    CCGGACGTGG ACCTGCCTGT TAGTCCGGCA GATGCTCCTG TGCCCAGCGT CACACATGAT 
    GACAGCATCT TACGGCCGAG CATGAAGCTG GTGAAGTTCA AGAAGGGCGA GAGCGTGGGC 
    TTGAGGCTCG CTGGGGGGAA CGACGTTGGG ATATTTGTGG CGGGAGTTCT GGAGGACAGT 
    CCTGCTGCTA AAGAGGGCCT GGAAGAGGGA GACCAGATTC TCAGGGTAAA TAATGTGGAT 
    TTTGCAAATA TCATCCGAGA GGAGGCAGTG CTGTTCCTGC TGGATCTGCC CAAAGGAGAG 
    GAGGTCACCA TCCTGGCCCA GAAGAAGAAA GACGTGTACC GCCGCATTGT AGAGTCTGAC 
    GTGGGAGACT CGTTCTACAT CAGGACTCAT TTTGAATATG AGAAGGAGTC GCCCTATGGC 
    CTGAGCTTCA ATAAGGGGGA GGTCTTCAGA GTCGTCGACA CCCTTTACAA TGGCAAACTG 
    GGCTCCTGGC TCGCCATCCG CATCGGCAAA AACCACCAGG AGGTGGAGCG GGGCATCATC 
    CCCAACAAGA ACAGGGCTGA GCAGTTATCA AGTGTCCAGT ACACTCTCCC AAAGACTCCA 
    GGAGGGGATC GCGCCGACTT CTGGAGATTC AGAGGCCTCC GCAGCTCCAA ACGAAACCTG 
    AGGAAGAGCA GAGAAGACCT GTCTGCTCAG CCGGTCCAAA CCAAATTCCC TGCTTATGAA 
    AGAGTGGTGC TTAGAGAAGC TGGTTTCTTG AGGCCAGTGG TCATTTTCGG ACCGATCGCG 
    GATGTGGCTA GAGAGAAGCT GTCCAGAGAG GAGACTGACC TCTTCGAACT TGCAAAGAGT 
    GAACCCAGAG ATGCGGGCAC AGACCAGCGC AGCTCCGGAA TCATCCGTCT CCATACCATT 
    AAACAGATCA TTGACCGAGA CAAGCACGCG GTGCTGGACA TCACCCCTAA TGCGGTGGAC 
    CGTCTGAACT ACGCTCAGTG GTACCCCATC GTGGTGTTTC TGAATCCAGA CAGCAAACAG 
    GGCGTCAAGA ACATGAGGAC CAGGCTCTGT CCCGAGTCCA GGAAGAGCGC CCGAAAGCTC 
    TACGAGCGTG CTCTCAAACT GAGGAAGAAC AACCATCACC TTTTCACCAC CACTATTAAC 
    TTGAACAACA TGAATGACGG GTGGTACGGT GCTTTGAAAG AGAGCATCCA GCAGCAGCAG 
    AACCAGTTGG TCTGGGTCTC AGAGGGCAAG GCCGACGGCG CTCCTGACGA TGACCTTGAC 
    ATCCATGACG ACCGCCTCTC CTACCTCTCG GCTCCGGGCA GCGAGTACAG CATGTACAGC 
    ACCGACAGCC GCCACACGTC CGACTATGAG GACACCGACA CGGAGGGTGG TGCCTACACC 
    GACCAGGAGC TGGACGAGAC CATGAACGAT GAGGTGGGCC TGCCTAGCGA GCCTGCCATC 
    ACACGCTCAT CTGAGCCTGT GCGAGAGGAC CCGCCAGTCA TTCAGGACGC AGCTGGATAC 
    CCCAGCTACC AGCATGCGGT ACCGCAACCT GAAGCGGTGA GCCGCATTGA CCCGGCCGGG 
    TTTAAGATGG CAGCGCCGCA GCAGCAAGCT GAGGCTGCTC TGGCCTCGCC GCCCCCTCCC 
    TCTGCAGCGG TCCAGCCCAC CGCAACACTA GCGGGTACGA ACCCCGAGGA GCCGCCTGGC 
    ACCCCTCAGG CCGACTCCCT TAACAGCCCC GTCCCTGTTC CCGACCCCCA GCCCGAACCT 
    GAGCTCGCTC AGCCCCCAAC ACACGACCCC CACCAGTCGC TTCCTCCTGG CCCAGATCCA 
    AAGATGTATA AAAAGGATCT GTACAGCATG GACGAGCCGG TGCGAGTGAC CCACGGCGTG 
    AAGCCTCCCC CTCCCCAGCA GCAGCAGCAG CCTTTAGGCC CCCCAACCTC GCTCTCCTAC 
    AGCCACCAGC CTGTCTACCA GGACCAACAG CCATACCGCC AGTACGACCA CCCGCCTTAT 
    GGCTATGACG GTGGCGGCTA CGCACAACCA AAGCCTCACA ACTACGACCC GCACCTGCAC 
    TACGACAACC GTGTGCCTCA CTATAACGAG CAGTGGCCCA CGTACGACCA GCAGCAGCAG 
    CAGCAGACTT CCCCCCAGCC GCCACCTGCG TCCGGCTTCC CTCAGGGCCA CCAGCCTCCG 
    CCTCCACCAC CGATGGGATA TGAGCCACGC TCCCCATACG AGGACGGACC TAACAGGGAT 
    TTCAGCCCTC CTCAGTCCCA GTATGACGGT GTGTCCCCAA TGGGCTACGA TCACCGGCCG 
    CGGCACCCCA AACCTGCACC GGTCCGATAT GAAGAGCCTC CACCTCCACC TCCTCCGGTG 
    TCCTACGATG CACGGTCACC TTTCGAACCC GAGCCGCATG CTTTCCCAGG CAACACACAT 
    CGCTCGCCAG ACCCACCGAA ACAGTATTAT GGAGACGCCG CTATGAGGCC CTTGTACAAC 
    CCTGGACCTC CGAACAGAGT GTACAAACAT GAGCCGGTGA ACTCTGAGCC TCCTGTACCG 
    CCTCCCAAAC CTGAATCTGT CCTGTCTCCA GGAGAACCGC CCCATTCTGC AGCTTCCAAA 
    CCATTGCCGC CTCCTCCCAG AGATGATGAC GATGATGATG ACGATGATGA TGACCCTGCC 
    ATGAAGCCCC AGTCTGTGCT CAACAGGGTC AAAATGTTCG AAAACAAACG CTCTGTGTCG 
    GTAGACCGAG CCAAGGACGC ACCTGACGTC ACTGGAATTA GGCCCACAGA TCTCCCCAAA 
    CCTGCGAGCA CCCCTGGACC TGTGCTCAAA GCCAACTCTC TCAGCAACCT GGAGCAGGAC 
    AAACCCTCCT ACAGAGCTCC AGAGCCGCAG AAACCTCAGA CGCGTGTTGG GGACGATGTG 
    GTTCGCTCCA ACCACTACGA CCCTGATGAT GATGAAGAGT ACTACAGAAA ACAGCTCTCA 
    TTTTTCGACC GCCGCAGTTT CGACAGTAAG GTCATGAATC CACCCAGCGC TGGAGTAAAC 
    CGCTTCCATG AGCCGCCCAA ACCACCCCAG ACACAGATCG CTTATCCTTT CGCTAGGACG 
    GAGTCTGTGG AGAAGGTGAG TCCTGTGGAC AAGAGATACG AGCCTCTGCC GCCAGTCAAC 
    CCTTCACCTG CTCCATACAG CCAGAACACA CCTGTCGCTC CGCCCACATC CCTGCCCAAA 
    CTCACCAACA TCGAGGTGAA CTCTCTGCCA GATCCTCACA GCTCTCCCAA AGCCAAACCG 
    GACCTGTCTG CTCTGAGAGC TCCGACCCGG GACGAGCCCA TCCAGAGCAG CTACTTGCCC 
    CCCAAGTCCA CCATCAATGG CATGGATGCT CCTCCCAAAA CACTCGGCGT GCCGACCAGC 
    TACAACCGCT ACGTCCCCAA GCCTTACACC AGCTCGGCGC GACCCTTCGA GCGCAAGTTC 
    GAGAGTCCGA AATTCAACCA CAACCTGCTT CCCAACGACA CGCAGTCCAA ACCCAGTGTC 
    AACAACAACC TGAAGCCTCA GATCTCCCCA CAACCCCAGG ACACGGACAG CGGCGTCGAC 
    ACCTTCACTC GCACCATGGA CAACAGGCCA AAATATCAGC ACAACAACAT CAACGCCATT 
    CCCAAAGCCG TCCCAGTCAG CCCCAGTGCA CTGGATGATG ATGAAGAAGA TGAGGGACAC 
    ACAGTTGTGG CCACGGCGCG GGGCATCTTT AACTGTAATG GCGGGGTGTT GAGCTCTATA 
    GAGACGGGCG TCAGCATCAT TATCCCACAG GGAGCCATTC CTGATAGTGT GGAGCAGGAG 
    ATCTACTTCA AAGTGTGCCG GGACAACAGC ATCCTGCCTC CTCTGGATAA GGAGAAAGGT 
    GAAACTCTGC TGAGTCCGCT GGTGATGTGT GGTCCTCACG GCCTGAAGTT CCTGAAGCCT 
    GTGGAGCTGC GCTTGCCACA CTGTGCGTCT ATGACCCCTG ATGGTTGGTC TTTTGCTCTA 
    AAATCCTCCG ACTCCTCGTC GGGTGACCCC AAATGCTGGC AAAACAAGTG TCTACCAGGA 
    GACCCCAATT ACCTGGTGGG CGCAAACTGT GTGTCTGTGC TGATCGACCA CTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354171.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209660.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID ODa57882
    Clone ID Related Accession (Same CDS sequence) XM_017354171.1
    Accession Version XM_017354171.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
    ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
    AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
    GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
    CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
    ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
    GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
    TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
    CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
    TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
    AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
    AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
    ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
    CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
    AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
    ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
    GCGTCTATGA CCCCTGATGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209660.1
    CDS115..6168
    Translation

    Target ORF information:

    RefSeq Version XM_017354171.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354171.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
    ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
    AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
    GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
    CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
    ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
    GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
    TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
    CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
    TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
    AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
    AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
    ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
    CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
    AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
    ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
    GCGTCTATGA CCCCTGATGG TGACCCCAAA TGCTGGCAAA ACAAGTGTCT ACCAGGAGAC 
    CCCAATTACC TGGTGGGCGC AAACTGTGTG TCTGTGCTGA TCGACCACTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354172.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209661.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID ODa57883
    Clone ID Related Accession (Same CDS sequence) XM_017354172.1 , XM_017354172.2
    Accession Version XM_017354172.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
    GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
    AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
    CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
    AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
    CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
    CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
    CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
    GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
    TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
    GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
    AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
    ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
    ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
    TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
    AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
    CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
    AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
    CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
    AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
    CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
    AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
    GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
    ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
    TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
    AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
    GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
    ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
    GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
    GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
    CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
    CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
    TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
    CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
    GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
    GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
    GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
    CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
    AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
    CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
    CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
    GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
    CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
    TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
    CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
    CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
    AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
    GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
    GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
    CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
    CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
    CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
    CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
    GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
    ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
    TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
    AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
    CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
    GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
    GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
    GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
    ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
    GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
    ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
    TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
    AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
    CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
    CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
    GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
    GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
    GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
    AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
    CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
    CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
    GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
    TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209661.1
    CDS115..6162
    Translation

    Target ORF information:

    RefSeq Version XM_017354172.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
    GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
    AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
    CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
    AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
    CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
    CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
    CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
    GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
    TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
    GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
    AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
    ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
    ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
    TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
    AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
    CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
    AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
    CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
    AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
    CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
    AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
    GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
    ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
    TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
    AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
    GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
    ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
    GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
    GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
    CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
    CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
    TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
    CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
    GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
    GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
    GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
    CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
    AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
    CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
    CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
    GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
    CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
    TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
    CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
    CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
    AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
    GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
    GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
    CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
    CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
    CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
    CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
    GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
    ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
    TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
    AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
    CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
    GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
    GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
    GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
    ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
    GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
    ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
    TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
    AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
    CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
    CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
    GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
    GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
    GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
    AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
    CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
    CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
    GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
    TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354172.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209661.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA.
    Price & Availability↑
    CloneID ODa57883
    Clone ID Related Accession (Same CDS sequence) XM_017354172.1 , XM_017354172.2
    Accession Version XM_017354172.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497369600000
    Organism Danio rerio(zebrafish)
    Product tight junction protein ZO-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
    GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
    AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
    CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
    AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
    CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
    CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
    CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
    GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
    TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
    GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
    AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
    ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
    ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
    TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
    AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
    CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
    AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
    CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
    AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
    CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
    AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
    GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
    ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
    TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
    AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
    GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
    ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
    GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
    GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
    CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
    CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
    TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
    CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
    GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
    GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
    GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
    CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
    AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
    CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
    CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
    GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
    CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
    TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
    CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
    CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
    AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
    GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
    GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
    CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
    CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
    CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
    CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
    GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
    ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
    TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
    AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
    CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
    GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
    GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
    GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
    ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
    GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
    ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
    TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
    AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
    CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
    CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
    GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
    GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
    GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
    AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
    CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
    CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
    GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
    TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209661.1
    CDS110..6157
    Translation

    Target ORF information:

    RefSeq Version XM_017354172.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354172.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGAAGCTGA TCGAGAGGTC AAAGGGAAAG CTGAAGATGG TGGTGCAGAG AGACGAGCGG 
    GCGACGCTGC TGAACATCCC CGATCTGGAC GACAGCATAC CTTCTGCTAA TGCCTCGGAC 
    AGAGATGATA TTTCAGACAT CCACTCCGTG GCCTCTGATC ATTCCAACCG ATCCCACGAC 
    CGGAAGAGGA GCAGCCGCTC TCGTTCTCCA GACCGCCGCT CCGAACCCTC AGACCACTCC 
    AGACACTCTC CACCACAGAT CAGCAACGGC AGGGCCACAG GGCCGACGGC TCAGAGAACG 
    CGGCCAGTTC ACAGGAGCCG TGATGAAGAC AGGATCTCCA AGGCCGGGCC GCTTCCTCTA 
    CCTGCTAAGA TGGTGGAGGA GATGCCGAAA GAGCAGAGCG CAGCCAGAGA AACAGAGAAA 
    CAGCTCCCCC CTCTGCCAGA GCCCAAACCT GTGTACGCTC AGCCGGGCCA GCCGGACGTG 
    GACCTGCCTG TTAGTCCGGC AGATGCTCCT GTGCCCAGCG TCACACATGA TGACAGCATC 
    TTACGGCCGA GCATGAAGCT GGTGAAGTTC AAGAAGGGCG AGAGCGTGGG CTTGAGGCTC 
    GCTGGGGGGA ACGACGTTGG GATATTTGTG GCGGGAGTTC TGGAGGACAG TCCTGCTGCT 
    AAAGAGGGCC TGGAAGAGGG AGACCAGATT CTCAGGGTAA ATAATGTGGA TTTTGCAAAT 
    ATCATCCGAG AGGAGGCAGT GCTGTTCCTG CTGGATCTGC CCAAAGGAGA GGAGGTCACC 
    ATCCTGGCCC AGAAGAAGAA AGACGTGTAC CGCCGCATTG TAGAGTCTGA CGTGGGAGAC 
    TCGTTCTACA TCAGGACTCA TTTTGAATAT GAGAAGGAGT CGCCCTATGG CCTGAGCTTC 
    AATAAGGGGG AGGTCTTCAG AGTCGTCGAC ACCCTTTACA ATGGCAAACT GGGCTCCTGG 
    CTCGCCATCC GCATCGGCAA AAACCACCAG GAGGTGGAGC GGGGCATCAT CCCCAACAAG 
    AACAGGGCTG AGCAGTTATC AAGTGTCCAG TACACTCTCC CAAAGACTCC AGGAGGGGAT 
    CGCGCCGACT TCTGGAGATT CAGAGGCCTC CGCAGCTCCA AACGAAACCT GAGGAAGAGC 
    AGAGAAGACC TGTCTGCTCA GCCGGTCCAA ACCAAATTCC CTGCTTATGA AAGAGTGGTG 
    CTTAGAGAAG CTGGTTTCTT GAGGCCAGTG GTCATTTTCG GACCGATCGC GGATGTGGCT 
    AGAGAGAAGC TGTCCAGAGA GGAGACTGAC CTCTTCGAAC TTGCAAAGAG TGAACCCAGA 
    GATGCGGGCA CAGACCAGCG CAGCTCCGGA ATCATCCGTC TCCATACCAT TAAACAGATC 
    ATTGACCGAG ACAAGCACGC GGTGCTGGAC ATCACCCCTA ATGCGGTGGA CCGTCTGAAC 
    TACGCTCAGT GGTACCCCAT CGTGGTGTTT CTGAATCCAG ACAGCAAACA GGGCGTCAAG 
    AACATGAGGA CCAGGCTCTG TCCCGAGTCC AGGAAGAGCG CCCGAAAGCT CTACGAGCGT 
    GCTCTCAAAC TGAGGAAGAA CAACCATCAC CTTTTCACCA CCACTATTAA CTTGAACAAC 
    ATGAATGACG GGTGGTACGG TGCTTTGAAA GAGAGCATCC AGCAGCAGCA GAACCAGTTG 
    GTCTGGGTCT CAGAGGGCAA GGCCGACGGC GCTCCTGACG ATGACCTTGA CATCCATGAC 
    GACCGCCTCT CCTACCTCTC GGCTCCGGGC AGCGAGTACA GCATGTACAG CACCGACAGC 
    CGCCACACGT CCGACTATGA GGACACCGAC ACGGAGGGTG GTGCCTACAC CGACCAGGAG 
    CTGGACGAGA CCATGAACGA TGAGGTGGGC CTGCCTAGCG AGCCTGCCAT CACACGCTCA 
    TCTGAGCCTG TGCGAGAGGA CCCGCCAGTC ATTCAGGACG CAGCTGGATA CCCCAGCTAC 
    CAGCATGCGG TACCGCAACC TGAAGCGGTG AGCCGCATTG ACCCGGCCGG GTTTAAGATG 
    GCAGCGCCGC AGCAGCAAGC TGAGGCTGCT CTGGCCTCGC CGCCCCCTCC CTCTGCAGCG 
    GTCCAGCCCA CCGCAACACT AGCGGGTACG AACCCCGAGG AGCCGCCTGG CACCCCTCAG 
    GCCGACTCCC TTAACAGCCC CGTCCCTGTT CCCGACCCCC AGCCCGAACC TGAGCTCGCT 
    CAGCCCCCAA CACACGACCC CCACCAGTCG CTTCCTCCTG GCCCAGATCC AAAGATGTAT 
    AAAAAGGATC TGTACAGCAT GGACGAGCCG GTGCGAGTGA CCCACGGCGT GAAGCCTCCC 
    CCTCCCCAGC AGCAGCAGCA GCCTTTAGGC CCCCCAACCT CGCTCTCCTA CAGCCACCAG 
    CCTGTCTACC AGGACCAACA GCCATACCGC CAGTACGACC ACCCGCCTTA TGGCTATGAC 
    GGTGGCGGCT ACGCACAACC AAAGCCTCAC AACTACGACC CGCACCTGCA CTACGACAAC 
    CGTGTGCCTC ACTATAACGA GCAGTGGCCC ACGTACGACC AGCAGCAGCA GCAGCAGACT 
    TCCCCCCAGC CGCCACCTGC GTCCGGCTTC CCTCAGGGCC ACCAGCCTCC GCCTCCACCA 
    CCGATGGGAT ATGAGCCACG CTCCCCATAC GAGGACGGAC CTAACAGGGA TTTCAGCCCT 
    CCTCAGTCCC AGTATGACGG TGTGTCCCCA ATGGGCTACG ATCACCGGCC GCGGCACCCC 
    AAACCTGCAC CGGTCCGATA TGAAGAGCCT CCACCTCCAC CTCCTCCGGT GTCCTACGAT 
    GCACGGTCAC CTTTCGAACC CGAGCCGCAT GCTTTCCCAG GCAACACACA TCGCTCGCCA 
    GACCCACCGA AACAGTATTA TGGAGACGCC GCTATGAGGC CCTTGTACAA CCCTGGACCT 
    CCGAACAGAG TGTACAAACA TGAGCCGGTG AACTCTGAGC CTCCTGTACC GCCTCCCAAA 
    CCTGAATCTG TCCTGTCTCC AGGAGAACCG CCCCATTCTG CAGCTTCCAA ACCATTGCCG 
    CCTCCTCCCA GAGATGATGA CGATGATGAT GACGATGATG ATGACCCTGC CATGAAGCCC 
    CAGTCTGTGC TCAACAGGGT CAAAATGTTC GAAAACAAAC GCTCTGTGTC GGTAGACCGA 
    GCCAAGGACG CACCTGACGT CACTGGAATT AGGCCCACAG ATCTCCCCAA ACCTGCGAGC 
    ACCCCTGGAC CTGTGCTCAA AGCCAACTCT CTCAGCAACC TGGAGCAGGA CAAACCCTCC 
    TACAGAGCTC CAGAGCCGCA GAAACCTCAG ACGCGTGTTG GGGACGATGT GGTTCGCTCC 
    AACCACTACG ACCCTGATGA TGATGAAGAG TACTACAGAA AACAGCTCTC ATTTTTCGAC 
    CGCCGCAGTT TCGACAGTAA GGTCATGAAT CCACCCAGCG CTGGAGTAAA CCGCTTCCAT 
    GAGCCGCCCA AACCACCCCA GACACAGATC GCTTATCCTT TCGCTAGGAC GGAGTCTGTG 
    GAGAAGGTGA GTCCTGTGGA CAAGAGATAC GAGCCTCTGC CGCCAGTCAA CCCTTCACCT 
    GCTCCATACA GCCAGAACAC ACCTGTCGCT CCGCCCACAT CCCTGCCCAA ACTCACCAAC 
    ATCGAGGTGA ACTCTCTGCC AGATCCTCAC AGCTCTCCCA AAGCCAAACC GGACCTGTCT 
    GCTCTGAGAG CTCCGACCCG GGACGAGCCC ATCCAGAGCA GCTACTTGCC CCCCAAGTCC 
    ACCATCAATG GCATGGATGC TCCTCCCAAA ACACTCGGCG TGCCGACCAG CTACAACCGC 
    TACGTCCCCA AGCCTTACAC CAGCTCGGCG CGACCCTTCG AGCGCAAGTT CGAGAGTCCG 
    AAATTCAACC ACAACCTGCT TCCCAACGAC ACGCAGTCCA AACCCAGTGT CAACAACAAC 
    CTGAAGCCTC AGATCTCCCC ACAACCCCAG GACACGGACA GCGGCGTCGA CACCTTCACT 
    CGCACCATGG ACAACAGGCC AAAATATCAG CACAACAACA TCAACGCCAT TCCCAAAGCC 
    GTCCCAGTCA GCCCCAGTGC ACTGGATGAT GATGAAGAAG ATGAGGGACA CACAGTTGTG 
    GCCACGGCGC GGGGCATCTT TAACTGTAAT GGCGGGGTGT TGAGCTCTAT AGAGACGGGC 
    GTCAGCATCA TTATCCCACA GGGAGCCATT CCTGATAGTG TGGAGCAGGA GATCTACTTC 
    AAAGTGTGCC GGGACAACAG CATCCTGCCT CCTCTGGATA AGGAGAAAGG TGAAACTCTG 
    CTGAGTCCGC TGGTGATGTG TGGTCCTCAC GGCCTGAAGT TCCTGAAGCC TGTGGAGCTG 
    CGCTTGCCAC ACTGTGCGTC TATGACCCCT GATGGTTGGT CTTTTGCTCT AAAATCCTCC 
    GACTCCTCGT CGGGTGACCC CAAATGCTGG CAAAACAAGT GTCTACCAGG AGACCCCAAT 
    TACCTGGTGG GCGCAAACTG TGTGTCTGTG CTGATCGACC ACTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TJP1B ( XM_017354173.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017209662.1 Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID ODa57884
    Clone ID Related Accession (Same CDS sequence) XM_017354173.1
    Accession Version XM_017354173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6033bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1466611200000
    Organism Danio rerio(Zebrafish)
    Product tight junction protein ZO-1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
    ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
    AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
    GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
    CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
    ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
    GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
    TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
    CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
    TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
    AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
    AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
    ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
    CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
    AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
    ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
    GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
    AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209662.1
    CDS115..6147
    Translation

    Target ORF information:

    RefSeq Version XM_017354173.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tight junction protein 1b (tjp1b), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017354173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCTCTTGT CTGTCTTTGG CGGACCTGTT GAAGAAGTGT TAGCAGGACT CCTCAAGCTG 
    AAGATGTTCG GAAGCGGCTC CAAAAAGTGG TTTATCAAGA AGAATAAACG GCAGAGGACT 
    AAAGTGCAGA GAAAACCGGC GAGCGGGAGG AGGAATAAAC GGGTGTCCAG AGTCCTGAAG 
    CGGCACAAGG AGGTGATTGT GAAGCAGCTG GACGTCAGTA AGGTGCTGCC GACTCTGGTG 
    TACGAGCGGG TGTTTACTCT GGGGGAATAT AAGGAGATTT TGGGACAGGA CTCGAGCGGT 
    AAACGTGCTG AGCTCTTCTT GGATTTGCTC TCCCAGAAGG AGCCCGCGGC CTTCAGCGCT 
    TTCTGTGGGG TGCTGGAGGA GGTGTGTCCG CACCTGCTCA CCTGCTTCCT GCTGGACGGC 
    ACAGATGGCT CAGCTGGAGA AAACAAGAAG CAAGGACAAA CTAAACCAGC GCACAGCCAG 
    CAGAACGCTC TGTCCAAGGG ACAAGACACC GTTTGTTTTC CACCGATGTA CACAGACCCA 
    CTTTTTGACA CTAGGGTCTT CCCCAAACAC GACAAAGACA AATACGGCCC TGCAGTTTAT 
    TATCCGAAGT TCAACGCCTA CATCCCCAAC CCAGACAAAG TCTCTGTGCT GGATGAATCT 
    GAGGGATCGG GCCACGGCAA ACCATCCCTG CGCAGAATCA AGGGCCGAAT CCACCGCAGC 
    AAGAGCCTGG ACAGCATCGA CCTGCTGGAC TCCAACAGTG CAGCAATGGA TGAGACGGTA 
    ATATGGGAAC AGCACACTGT AACTCTACAC AGGGCTCCAG GGTTTGGCTT CGGGATCGCC 
    ATCTCCGGCG GACGGGACAA CCCTCACTTC CAGAGCGGGG AGACGTCCAT CGTCATATCA 
    GATGTGCTGA AGGGAGGCCC CGCCGAGGGC CTGCTGCAGG AGAATGACAG AGTGGTGATG 
    GTCAACGCCG TCTCCATGGA CAATGTGGAT CACGCTTACG CAGTGCAGCA GCTCCGCAAA 
    AGTGGCAAGA ATGCAAAAAT AACCATCAGG AGAAAGCGTA AGGTGCAGAT CCCGTCAGGC 
    CGGCCGGGTG AACGCGAGAC TATGTCAGAG CATGACGAGG ACGACGACAG CTACGAGGAT 
    GAGATCTACG AGTCCCGGAG CGCGCGAAGC GGACCCAGCG CCTACAGTGG AGGTGGAGGC 
    GGCGGCGGAG GTGGAGGAGC CTCGGGACGG AGGAGCGGCA GAGGCGACAG GCTGAGTGGG 
    AGGAGGGAGC GGGACAGAGA GCGCAGCGGC TCCAGAGAGA GGAGTCTGTC CCCGCGCTCG 
    GACCGCCACT CTGTGTCCTC CAACCTCCCC CCACGGCCAG CCAAAGTCAC ACTCGTCAAA 
    TCCCGCAAGA ATGAAGCAGA ATACGGGCTT CGCCTGGCCA GCCACATCTT CGTGAAGGAC 
    ATTTCCCCCG AGAGCCTGGC AGCCCGAGAC GGAAACATCC AAGAGGGAGA CGTGGTGCTT 
    AAGATTAATG GCACGGTAAC AGAGAATTTG TCCCTGATCG ATGCTAAGAA GCTGATCGAG 
    AGGTCAAAGG GAAAGCTGAA GATGGTGGTG CAGAGAGACG AGCGGGCGAC GCTGCTGAAC 
    ATCCCCGATC TGGACGACAG CATACCTTCT GCTAATGCCT CGGACAGAGA TGATATTTCA 
    GACATCCACT CCGTGGCCTC TGATCATTCC AACCGATCCC ACGACCGGAA GAGGAGCAGC 
    CGCTCTCGTT CTCCAGACCG CCGCTCCGAA CCCTCAGACC ACTCCAGACA CTCTCCACCA 
    CAGATCAGCA ACGGCAGGGC CACAGGGCCG ACGGCTCAGA GAACGCGGCC AGTTCACAGG 
    AGCCGTGATG AAGACAGGAT CTCCAAGGCC GGGCCGCTTC CTCTACCTGC TAAGATGGTG 
    GAGGAGATGC CGAAAGAGCA GAGCGCAGCC AGAGAAACAG AGAAACAGCT CCCCCCTCTG 
    CCAGAGCCCA AACCTGTGTA CGCTCAGCCG GGCCAGCCGG ACGTGGACCT GCCTGTTAGT 
    CCGGCAGATG CTCCTGTGCC CAGCGTCACA CATGATGACA GCATCTTACG GCCGAGCATG 
    AAGCTGGTGA AGTTCAAGAA GGGCGAGAGC GTGGGCTTGA GGCTCGCTGG GGGGAACGAC 
    GTTGGGATAT TTGTGGCGGG AGTTCTGGAG GACAGTCCTG CTGCTAAAGA GGGCCTGGAA 
    GAGGGAGACC AGATTCTCAG GGTAAATAAT GTGGATTTTG CAAATATCAT CCGAGAGGAG 
    GCAGTGCTGT TCCTGCTGGA TCTGCCCAAA GGAGAGGAGG TCACCATCCT GGCCCAGAAG 
    AAGAAAGACG TGTACCGCCG CATTGTAGAG TCTGACGTGG GAGACTCGTT CTACATCAGG 
    ACTCATTTTG AATATGAGAA GGAGTCGCCC TATGGCCTGA GCTTCAATAA GGGGGAGGTC 
    TTCAGAGTCG TCGACACCCT TTACAATGGC AAACTGGGCT CCTGGCTCGC CATCCGCATC 
    GGCAAAAACC ACCAGGAGGT GGAGCGGGGC ATCATCCCCA ACAAGAACAG GGCTGAGCAG 
    TTATCAAGTG TCCAGTACAC TCTCCCAAAG ACTCCAGGAG GGGATCGCGC CGACTTCTGG 
    AGATTCAGAG GCCTCCGCAG CTCCAAACGA AACCTGAGGA AGAGCAGAGA AGACCTGTCT 
    GCTCAGCCGG TCCAAACCAA ATTCCCTGCT TATGAAAGAG TGGTGCTTAG AGAAGCTGGT 
    TTCTTGAGGC CAGTGGTCAT TTTCGGACCG ATCGCGGATG TGGCTAGAGA GAAGCTGTCC 
    AGAGAGGAGA CTGACCTCTT CGAACTTGCA AAGAGTGAAC CCAGAGATGC GGGCACAGAC 
    CAGCGCAGCT CCGGAATCAT CCGTCTCCAT ACCATTAAAC AGATCATTGA CCGAGACAAG 
    CACGCGGTGC TGGACATCAC CCCTAATGCG GTGGACCGTC TGAACTACGC TCAGTGGTAC 
    CCCATCGTGG TGTTTCTGAA TCCAGACAGC AAACAGGGCG TCAAGAACAT GAGGACCAGG 
    CTCTGTCCCG AGTCCAGGAA GAGCGCCCGA AAGCTCTACG AGCGTGCTCT CAAACTGAGG 
    AAGAACAACC ATCACCTTTT CACCACCACT ATTAACTTGA ACAACATGAA TGACGGGTGG 
    TACGGTGCTT TGAAAGAGAG CATCCAGCAG CAGCAGAACC AGTTGGTCTG GGTCTCAGAG 
    GGCAAGGCCG ACGGCGCTCC TGACGATGAC CTTGACATCC ATGACGACCG CCTCTCCTAC 
    CTCTCGGCTC CGGGCAGCGA GTACAGCATG TACAGCACCG ACAGCCGCCA CACGTCCGAC 
    TATGAGGACA CCGACACGGA GGGTGGTGCC TACACCGACC AGGAGCTGGA CGAGACCATG 
    AACGATGAGG TGGGCCTGCC TAGCGAGCCT GCCATCACAC GCTCATCTGA GCCTGTGCGA 
    GAGGACCCGC CAGTCATTCA GGACGCAGCT GGATACCCCA GCTACCAGCA TGCGGTACCG 
    CAACCTGAAG CGGTGAGCCG CATTGACCCG GCCGGGTTTA AGATGGCAGC GCCGCAGCAG 
    CAAGCTGAGG CTGCTCTGGC CTCGCCGCCC CCTCCCTCTG CAGCGGTCCA GCCCACCGCA 
    ACACTAGCGG GTACGAACCC CGAGGAGCCG CCTGGCACCC CTCAGGCCGA CTCCCTTAAC 
    AGCCCCGTCC CTGTTCCCGA CCCCCAGCCC GAACCTGAGC TCGCTCAGCC CCCAACACAC 
    GACCCCCACC AGTCGCTTCC TCCTGGCCCA GATCCAAAGA TGTATAAAAA GGATCTGTAC 
    AGCATGGACG AGCCGGTGCG AGTGACCCAC GGCGTGAAGC CTCCCCCTCC CCAGCAGCAG 
    CAGCAGCCTT TAGGCCCCCC AACCTCGCTC TCCTACAGCC ACCAGCCTGT CTACCAGGAC 
    CAACAGCCAT ACCGCCAGTA CGACCACCCG CCTTATGGCT ATGACGGTGG CGGCTACGCA 
    CAACCAAAGC CTCACAACTA CGACCCGCAC CTGCACTACG ACAACCGTGT GCCTCACTAT 
    AACGAGCAGT GGCCCACGTA CGACCAGCAG CAGCAGCAGC AGACTTCCCC CCAGCCGCCA 
    CCTGCGTCCG GCTTCCCTCA GGGCCACCAG CCTCCGCCTC CACCACCGAT GGGATATGAG 
    CCACGCTCCC CATACGAGGA CGGACCTAAC AGGGATTTCA GCCCTCCTCA GTCCCAGTAT 
    GACGGTGTGT CCCCAATGGG CTACGATCAC CGGCCGCGGC ACCCCAAACC TGCACCGGTC 
    CGATATGAAG AGCCTCCACC TCCACCTCCT CCGGTGTCCT ACGATGCACG GTCACCTTTC 
    GAACCCGAGC CGCATGCTTT CCCAGGCAAC ACACATCGCT CGCCAGACCC ACCGAAACAG 
    TATTATGGAG ACGCCGCTAT GAGGCCCTTG TACAACCCTG GACCTCCGAA CAGAGTGTAC 
    AAACATGAGC CGGTGAACTC TGAGCCTCCT GTACCGCCTC CCAAACCTGA ATCTGTCCTG 
    TCTCCAGGAG AACCGCCCCA TTCTGCAGCT TCCAAACCAT TGCCGCCTCC TCCCAGAGAT 
    GATGACGATG ATGATGACGA TGATGATGAC CCTGCCATGA AGCCCCAGTC TGTGCTCAAC 
    AGGGTCAAAA TGTTCGAAAA CAAACGCTCT GTGTCGGTAG ACCGAGCCAA GGACGCACCT 
    GACGTCACTG GAATTAGGCC CACAGATCTC CCCAAACCTG CGAGCACCCC TGGACCTGTG 
    CTCAAAGCCA ACTCTCTCAG CAACCTGGAG CAGGACAAAC CCTCCTACAG AGCTCCAGAG 
    CCGCAGAAAC CTCAGACGCG TGTTGGGGAC GATGTGGTTC GCTCCAACCA CTACGACCCT 
    GATGATGATG AAGAGTACTA CAGAAAACAG CTCTCATTTT TCGACCGCCG CAGTTTCGAC 
    AGTAAGGTCA TGAATCCACC CAGCGCTGGA GTAAACCGCT TCCATGAGCC GCCCAAACCA 
    CCCCAGACAC AGATCGCTTA TCCTTTCGCT AGGACGGAGT CTGTGGAGAA GGTGAGTCCT 
    GTGGACAAGA GATACGAGCC TCTGCCGCCA GTCAACCCTT CACCTGCTCC ATACAGCCAG 
    AACACACCTG TCGCTCCGCC CACATCCCTG CCCAAACTCA CCAACATCGA GGTGAACTCT 
    CTGCCAGATC CTCACAGCTC TCCCAAAGCC AAACCGGACC TGTCTGCTCT GAGAGCTCCG 
    ACCCGGGACG AGCCCATCCA GAGCAGCTAC TTGCCCCCCA AGTCCACCAT CAATGGCATG 
    GATGCTCCTC CCAAAACACT CGGCGTGCCG ACCAGCTACA ACCGCTACGT CCCCAAGCCT 
    TACACCAGCT CGGCGCGACC CTTCGAGCGC AAGTTCGAGA GTCCGAAATT CAACCACAAC 
    CTGCTTCCCA ACGACACGCA GTCCAAACCC AGTGTCAACA ACAACCTGAA GCCTCAGATC 
    TCCCCACAAC CCCAGGACAC GGACAGCGGC GTCGACACCT TCACTCGCAC CATGGACAAC 
    AGGCCAAAAT ATCAGCACAA CAACATCAAC GCCATTCCCA AAGCCGTCCC AGTCAGCCCC 
    AGTGCACTGG ATGATGATGA AGAAGATGAG GGACACACAG TTGTGGCCAC GGCGCGGGGC 
    ATCTTTAACT GTAATGGCGG GGTGTTGAGC TCTATAGAGA CGGGCGTCAG CATCATTATC 
    CCACAGGGAG CCATTCCTGA TAGTGTGGAG CAGGAGATCT ACTTCAAAGT GTGCCGGGAC 
    AACAGCATCC TGCCTCCTCT GGATAAGGAG AAAGGTGAAA CTCTGCTGAG TCCGCTGGTG 
    ATGTGTGGTC CTCACGGCCT GAAGTTCCTG AAGCCTGTGG AGCTGCGCTT GCCACACTGT 
    GACCCCAAAT GCTGGCAAAA CAAGTGTCTA CCAGGAGACC CCAATTACCT GGTGGGCGCA 
    AACTGTGTGT CTGTGCTGAT CGACCACTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

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