NWD1 cDNA ORF clone, Felis catus(domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	NWD1
Entrez Gene ID	101095083
Full Name	NACHT and WD repeat domain containing 1
Gene Type	protein-coding
Organism	Felis catus(domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019819124.1	XP_019674683.1	NACHT domain- and WD repeat-containing protein 1 isoform X1
XM_019819120.1	XP_019674679.1	NACHT domain- and WD repeat-containing protein 1 isoform X1
XM_019819129.1	XP_019674688.1	NACHT domain- and WD repeat-containing protein 1 isoform X3
XM_011290359.2	XP_011288661.1	NACHT domain- and WD repeat-containing protein 1 isoform X2
XM_019819134.1	XP_019674693.1	NACHT domain- and WD repeat-containing protein 1 isoform X4
XM_019819120.2	XP_019674679.2	LOW QUALITY PROTEIN: NACHT domain- and WD repeat-containing protein 1

Pathways from BioSystems
Gene Ontology

The following NWD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NWD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb38249	XM_019819134.1 Latest version!	Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFb32181	XM_011290359.2 Latest version!	Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb38247	XM_019819120.1 Latest version!	Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb38247	XM_019819124.1 Latest version!	Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb38248	XM_019819129.1 Latest version!	Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb71700	XM_019819120.2 Latest version!	Felis catus NACHT and WD repeat domain containing 1 (NWD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NWD1 ( XM_019819134.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019674693.1 Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OFb38249
Clone ID Related Accession (Same CDS sequence)	XM_019819134.1
Accession Version	XM_019819134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018724.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACC GGCTCCCAGA TGGCTGCCTG GACACAGACG CCCAGAACCT TCTCCGTGGC 
CTCAAAGGAC GCATTGCTGA CGTGCAACCG GGAGTTCTCA GGGCTCACCA CCTGCCGTGG 
AGCCGAGACC TGGTGAACCC TAAAAACAAG GCTCATGCTC GCTACCTGAA GGAGCTGGGG 
GAACAGTTTG TGGCCAGGGC TAGCCACCAG GTCCTGGAGC ACCTCCGGGA GCTGGAGGCG 
GGCAGGCAGG AGTTGGCGTG GCTCTATCAG GAGATCCGCC ACCACCTGGG GCTGAGTGCC 
GAGGCCAATC GGACTTTCTG TGGGCGCCAG GAGCTCCTGG CCCAGCTGGG GCAGCGGCTC 
CAGCAGAGCG ACAGCCACCC CCACTCTCCC CTGGTGCTCT TTGGGCCCCC CGGCATCGGA 
AAGACAGCTC TGATGTGCAA ACTGGCGGAA CAAATGCCAG GCCTGCTTGG CCGCAAGACG 
GTGACCGTCC TGCGGCTTCT GGGAGCGTCA CAGATGAGCT CCGACGCCCG AGGCCTGCTC 
CAGAGCATCT GCGCCCAGGT GTGCCTGGCC TACGGGTTGC CCCTGCCCCC TGCCCAGGTC 
CTGGAGGCCC ACGCCAGGGT GGTGCAGTTT TTCCACACCC TCCTCCACAC CGTCTCCTGC 
CGCAACTTCG AGTCCCTTGT GATCCTGTTG GATTCCATGG ACGATGTGGA TTCCATCTGC 
CGCGCCCGGA GGGTCCCCTG GCTGCCTCCC AAATGCCCCC CGAGGATCCA CCTTATCCTC 
TCAGCCTGCT CAGGGCGCCA GGGGATTCTA GACACCGTGC GACAGACGCT GACGGACCCG 
GAGGCCTACT GGGAGGTGAA ACCCCTCTCC GGAAACCAAG GGCAAGAGAT GATCCAGCTC 
CTGCTGGCCG CCTCGAGGAG GACGCTGAGC CCAGGGCAGA GGGAACTGCT CTGGGCCAGC 
CTCCCAGAGT GCGGGCACCC AGGGCGGCTG AGGCTGGCCT TCGAGGAGGC CCGGAAATGG 
GCCTCCTTCA CCATACCTGC CCCCCTGGCC TCCACTGCCA AGGAAGCCAT GCACCAACTG 
TGTGCCCGCC TGGAGCAGAC ACACGGGCAG GTCCTGGTGG CCCACGTGCT GGGCTACATC 
GCGTCTTCCC GGCATGGGCT CTCAGAGGCA GAGCTGAAGG ACGTCCTGTC CTTGGATGAC 
GAGGTCCTGC AGGTGGTCTA CCGGGACTGG ACCCCGCCCA GCAAGGAGCT ACTGCGTTTT 
CCACCGCTGC TCTGGGTGAG GCTTCGCCGG GATCTGGGCC ACTGCTTGGC CCGGCGGCCC 
GTGGACGGCT TCACGCTCCT GGCCATTGCC CACAGGCAGC TGGCCGAGGT GGTCCAAGAA 
CGCTACCTGC CCGGGGTCGA GAGAGCCAAG AGGCACGGGG TCTTGGCTGA CTTCTTCTCA 
GGGGCCTGGA GCCAGGGCAC CAAGAAGCTC GTCACCCTGC CGCTCGTGGG GAAGCCCCTG 
AACCTGGACC GCATGGTGGC CCCTCAGCCC CTGTGGTTCT CGGACACGGT CGCAAATCTG 
CGAAAGCTGA AGGAGATGCC TCATCACCTG GTCCACTCAG GCCGCCTTGA AACGCTGAAG 
CAGGAGGTTC TGGGCAGCAT GAACTGGATT TCCTGCCGCG GCATCTCTGG AGGCATCGAG 
AGCCTGTTGG ACGACTTTGA CCTGTATGCC CCTTACCTGG ACTGTCCGGA GGTCGGCCTG 
GTCCGGGAAG CCCTCCAGCT GTGCCGCCCG GCCCTGGAGC TCCGAGGCAT GGAGAGGAGC 
ATCCTATATA CAGAACTCCT GGCCAGACTC CATTTCTTCA CGACCTCACA TCCGGCGCTG 
GTGGGACAGC TGTGCCAACA GGCCCAGAAC TGGTTCCGGA TGTGCCCACA TCCTGTGCTG 
GTACCCCTCA CGGGATTCCT CCAGCCCCCG GGAGGACCCC TCCGGGCAAC CCTCACCGGC 
TGTCACAAGG GCATCACGGC CATGGCGTGG AGCGTGGAAG AGAGGCTGCT GGTTGTCGGC 
ACCCAGGATG GCACCGTGGC TGTGTGGGAC ACGGAAGAAC ATCAGGTGAT CCATATGCAC 
ATGGGACACA CAGGAGAAGT GAGGTATGTG AAGGTGTTTG CCAAAGGGAC ACTTGCCCTC 
TCTGCCTCAA AGGACCATAC ACTGCGCTTG TGGAATCTAC TGACTGGCCA GGAGAAATTC 
ACCATTTGGG ATGGAGGCTC AAAGGATCCC ATGCAACCTG AGACCTGGAG TCTTCACTTG 
GATGAAACAA AGAAGGTTGT GTATTTGGCA TCTGGCTCAA AGGTCAGTGC GTGGCATCTA 
GAAACTGCAG AACTGGTTTT CCAGATCCTG GGAGATCCCT CTGACTGCTG GGTGTATGCA 
GGAGTGCTGG CTTCCCGGGC CACGCTGCTG ACCGTGTCCC AGGGTGGCGT GGCCGGCCTG 
TGGAGCTCAG CCACAGGAAA GCCGCAGGGG AGGCAACGTT TATCCAGCAT CAAGGAAGAA 
ACGCCTACCT GTGGGGTCTC GCTCCAGAAG CAGGGGAAGA TGGTCACCGG GTTCAGCAAG 
GGCTCCGTCT CCTTGATTTC CCCCGAAGGA GACAGCCTGC TGGAGAAGCT TCCGGAAGCT 
GTGAGGTTCC TGGTAGTCTC TGAAGATGAG TCCCTTCTCG CTGCAGGGTT TGGAAGATCC 
GTCCGGATAT TCTTGGCAGA CTCACAGGGG TTTCATCGAT TCATGGCCAC CGACCTTGAA 
CACGAGGACA CCGTGGAGAC AGCCAGTTTT GGTCCTGAGA ACAATCTGAT TGTCACCGGG 
TCCCGGGACG CCCTCATTCA GGTGTGGAGT CTGTCGGAGC AGGGGACCCT GCTGGAAGTT 
CTGGAAGGCG TCGGGGCCCC CGTGAGCCTG TTAGTCTGGG GTGGGGCCTT GGTGGCATCT 
GCTTCCCAGC AGTCCTCGTC TTTCAAAGTC TGGGATCTCA CGTCTGTGCG GCAGCCGAGG 
GCCTCTACCC CGTTTCTGGA TCGCACTGGC CTCACTGCGG TATCACACGA TGGAAGCTAT 
GTTTACTTCC CCAAGATTGG GGACAAGAAC AAAGTCACCA TTTGGGACTT AGCAGAAGGT 
GAGGAGCAGG ACGTCCTGGA TGCCTCCAGT GAGGTCAGAT GTCTGGAGGT GGCCCAGCAG 
GCCAATCTCC TTTTCACTGG CCTCGTTTCG GGGGTCGTCC TGGTGTTCCC CCTGAATTCC 
AGACAGGACG TGATGTGCAT CCCCCCTCCC GAGGCCCGGA AAGCCATCAA CTGCATGGCC 
CTCAGTCAAG GAGAGGAGCG CCTGGCCGTC GCCTATGACA GCATCATCCT GGTGCTGGAC 
ATCAGCCCCG GGGATCCCTG TCCGGTTGTC GACGGGCCAA CATACACCTT CTACACCCAG 
CTGCCTGAGA CCATCTCCAG CGTGGCCGTC CTGGCTGACT ACCGTGTGAT CTACGGCATG 
ACCGACGGTG AGCTCTTCCT TTACGAGTGT GCGAATTCCA AAGTGTTCCC TCTGGAGGCC 
CACGGGAGCC GGGTCACCTG TGTGCAGGTC AGCCACAAGG AGCAGCTGGC GGTCAGCGGA 
TCCGAGGAAG CCCTGCTGTG CCTCTGGGAC CTGCAGGCCT GCAAGTGGAG GTTCGAGACG 
AGCTACACGA ATTCTTACTG TAGAGGAGTC CAGTGTGTCT GCTTCTCCAA GGATGACAAG 
TACGTGTATG CGGGCTTGAA GGACCGCTCC ATCATCGTCT GGAGTGTGCT GGATGGCACC 
CTGCTGGCTG TCCAGTTTGT CCACGCCGTG GTGAACAGAA TCATCCCAAC TTCCAGTGGC 
TTCATCGCCC CCACGAGACA CGGCTATCTC ATCCGGGAGC GTTTCCAGGA CCCTTCGTCA 
AGAGCCTCGC AGCAGGACCC TCTCAAGAAC TTCAAGAAGG CAGTGTGGAA GGTCAAATCC 
AGGCAGAGAG ACGAAGCGGC GGCTGTCGCA GGAGGCCCCC AGGACTCGAG ATCAGCAAGT 
GTCCAGGGAA ATGCGTGCAA ATCGAACAAA CGCTCACAAG TCTGCCTGCT CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019674693.1
CDS	237..4313
Translation	MVDRLPDGCLDTDAQNLLRGLKGRIADVQPGVLRAHHLPWSRDLVNPKNKAHARYLKELGEQFVARASHQVLEHLRELEAGRQELAWLYQEIRHHLGLSAEANRTFCGRQELLAQLGQRLQQSDSHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGASQMSSDARGLLQSICAQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPKCPPRIHLILSACSGRQGILDTVRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAMHQLCARLEQTHGQVLVAHVLGYIASSRHGLSEAELKDVLSLDDEVLQVVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLAEVVQERYLPGVERAKRHGVLADFFSGAWSQGTKKLVTLPLVGKPLNLDRMVAPQPLWFSDTVANLRKLKEMPHHLVHSGRLETLKQEVLGSMNWISCRGISGGIESLLDDFDLYAPYLDCPEVGLVREALQLCRPALELRGMERSILYTELLARLHFFTTSHPALVGQLCQQAQNWFRMCPHPVLVPLTGFLQPPGGPLRATLTGCHKGITAMAWSVEERLLVVGTQDGTVAVWDTEEHQVIHMHMGHTGEVRYVKVFAKGTLALSASKDHTLRLWNLLTGQEKFTIWDGGSKDPMQPETWSLHLDETKKVVYLASGSKVSAWHLETAELVFQILGDPSDCWVYAGVLASRATLLTVSQGGVAGLWSSATGKPQGRQRLSSIKEETPTCGVSLQKQGKMVTGFSKGSVSLISPEGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVETASFGPENNLIVTGSRDALIQVWSLSEQGTLLEVLEGVGAPVSLLVWGGALVASASQQSSSFKVWDLTSVRQPRASTPFLDRTGLTAVSHDGSYVYFPKIGDKNKVTIWDLAEGEEQDVLDASSEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSQGEERLAVAYDSIILVLDISPGDPCPVVDGPTYTFYTQLPETISSVAVLADYRVIYGMTDGELFLYECANSKVFPLEAHGSRVTCVQVSHKEQLAVSGSEEALLCLWDLQACKWRFETSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSSGFIAPTRHGYLIRERFQDPSSRASQQDPLKNFKKAVWKVKSRQRDEAAAVAGGPQDSRSASVQGNACKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_019819134.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019819134.1

ORF Insert Sequence:

ATGGTGGACC GGCTCCCAGA TGGCTGCCTG GACACAGACG CCCAGAACCT TCTCCGTGGC 
CTCAAAGGAC GCATTGCTGA CGTGCAACCG GGAGTTCTCA GGGCTCACCA CCTGCCGTGG 
AGCCGAGACC TGGTGAACCC TAAAAACAAG GCTCATGCTC GCTACCTGAA GGAGCTGGGG 
GAACAGTTTG TGGCCAGGGC TAGCCACCAG GTCCTGGAGC ACCTCCGGGA GCTGGAGGCG 
GGCAGGCAGG AGTTGGCGTG GCTCTATCAG GAGATCCGCC ACCACCTGGG GCTGAGTGCC 
GAGGCCAATC GGACTTTCTG TGGGCGCCAG GAGCTCCTGG CCCAGCTGGG GCAGCGGCTC 
CAGCAGAGCG ACAGCCACCC CCACTCTCCC CTGGTGCTCT TTGGGCCCCC CGGCATCGGA 
AAGACAGCTC TGATGTGCAA ACTGGCGGAA CAAATGCCAG GCCTGCTTGG CCGCAAGACG 
GTGACCGTCC TGCGGCTTCT GGGAGCGTCA CAGATGAGCT CCGACGCCCG AGGCCTGCTC 
CAGAGCATCT GCGCCCAGGT GTGCCTGGCC TACGGGTTGC CCCTGCCCCC TGCCCAGGTC 
CTGGAGGCCC ACGCCAGGGT GGTGCAGTTT TTCCACACCC TCCTCCACAC CGTCTCCTGC 
CGCAACTTCG AGTCCCTTGT GATCCTGTTG GATTCCATGG ACGATGTGGA TTCCATCTGC 
CGCGCCCGGA GGGTCCCCTG GCTGCCTCCC AAATGCCCCC CGAGGATCCA CCTTATCCTC 
TCAGCCTGCT CAGGGCGCCA GGGGATTCTA GACACCGTGC GACAGACGCT GACGGACCCG 
GAGGCCTACT GGGAGGTGAA ACCCCTCTCC GGAAACCAAG GGCAAGAGAT GATCCAGCTC 
CTGCTGGCCG CCTCGAGGAG GACGCTGAGC CCAGGGCAGA GGGAACTGCT CTGGGCCAGC 
CTCCCAGAGT GCGGGCACCC AGGGCGGCTG AGGCTGGCCT TCGAGGAGGC CCGGAAATGG 
GCCTCCTTCA CCATACCTGC CCCCCTGGCC TCCACTGCCA AGGAAGCCAT GCACCAACTG 
TGTGCCCGCC TGGAGCAGAC ACACGGGCAG GTCCTGGTGG CCCACGTGCT GGGCTACATC 
GCGTCTTCCC GGCATGGGCT CTCAGAGGCA GAGCTGAAGG ACGTCCTGTC CTTGGATGAC 
GAGGTCCTGC AGGTGGTCTA CCGGGACTGG ACCCCGCCCA GCAAGGAGCT ACTGCGTTTT 
CCACCGCTGC TCTGGGTGAG GCTTCGCCGG GATCTGGGCC ACTGCTTGGC CCGGCGGCCC 
GTGGACGGCT TCACGCTCCT GGCCATTGCC CACAGGCAGC TGGCCGAGGT GGTCCAAGAA 
CGCTACCTGC CCGGGGTCGA GAGAGCCAAG AGGCACGGGG TCTTGGCTGA CTTCTTCTCA 
GGGGCCTGGA GCCAGGGCAC CAAGAAGCTC GTCACCCTGC CGCTCGTGGG GAAGCCCCTG 
AACCTGGACC GCATGGTGGC CCCTCAGCCC CTGTGGTTCT CGGACACGGT CGCAAATCTG 
CGAAAGCTGA AGGAGATGCC TCATCACCTG GTCCACTCAG GCCGCCTTGA AACGCTGAAG 
CAGGAGGTTC TGGGCAGCAT GAACTGGATT TCCTGCCGCG GCATCTCTGG AGGCATCGAG 
AGCCTGTTGG ACGACTTTGA CCTGTATGCC CCTTACCTGG ACTGTCCGGA GGTCGGCCTG 
GTCCGGGAAG CCCTCCAGCT GTGCCGCCCG GCCCTGGAGC TCCGAGGCAT GGAGAGGAGC 
ATCCTATATA CAGAACTCCT GGCCAGACTC CATTTCTTCA CGACCTCACA TCCGGCGCTG 
GTGGGACAGC TGTGCCAACA GGCCCAGAAC TGGTTCCGGA TGTGCCCACA TCCTGTGCTG 
GTACCCCTCA CGGGATTCCT CCAGCCCCCG GGAGGACCCC TCCGGGCAAC CCTCACCGGC 
TGTCACAAGG GCATCACGGC CATGGCGTGG AGCGTGGAAG AGAGGCTGCT GGTTGTCGGC 
ACCCAGGATG GCACCGTGGC TGTGTGGGAC ACGGAAGAAC ATCAGGTGAT CCATATGCAC 
ATGGGACACA CAGGAGAAGT GAGGTATGTG AAGGTGTTTG CCAAAGGGAC ACTTGCCCTC 
TCTGCCTCAA AGGACCATAC ACTGCGCTTG TGGAATCTAC TGACTGGCCA GGAGAAATTC 
ACCATTTGGG ATGGAGGCTC AAAGGATCCC ATGCAACCTG AGACCTGGAG TCTTCACTTG 
GATGAAACAA AGAAGGTTGT GTATTTGGCA TCTGGCTCAA AGGTCAGTGC GTGGCATCTA 
GAAACTGCAG AACTGGTTTT CCAGATCCTG GGAGATCCCT CTGACTGCTG GGTGTATGCA 
GGAGTGCTGG CTTCCCGGGC CACGCTGCTG ACCGTGTCCC AGGGTGGCGT GGCCGGCCTG 
TGGAGCTCAG CCACAGGAAA GCCGCAGGGG AGGCAACGTT TATCCAGCAT CAAGGAAGAA 
ACGCCTACCT GTGGGGTCTC GCTCCAGAAG CAGGGGAAGA TGGTCACCGG GTTCAGCAAG 
GGCTCCGTCT CCTTGATTTC CCCCGAAGGA GACAGCCTGC TGGAGAAGCT TCCGGAAGCT 
GTGAGGTTCC TGGTAGTCTC TGAAGATGAG TCCCTTCTCG CTGCAGGGTT TGGAAGATCC 
GTCCGGATAT TCTTGGCAGA CTCACAGGGG TTTCATCGAT TCATGGCCAC CGACCTTGAA 
CACGAGGACA CCGTGGAGAC AGCCAGTTTT GGTCCTGAGA ACAATCTGAT TGTCACCGGG 
TCCCGGGACG CCCTCATTCA GGTGTGGAGT CTGTCGGAGC AGGGGACCCT GCTGGAAGTT 
CTGGAAGGCG TCGGGGCCCC CGTGAGCCTG TTAGTCTGGG GTGGGGCCTT GGTGGCATCT 
GCTTCCCAGC AGTCCTCGTC TTTCAAAGTC TGGGATCTCA CGTCTGTGCG GCAGCCGAGG 
GCCTCTACCC CGTTTCTGGA TCGCACTGGC CTCACTGCGG TATCACACGA TGGAAGCTAT 
GTTTACTTCC CCAAGATTGG GGACAAGAAC AAAGTCACCA TTTGGGACTT AGCAGAAGGT 
GAGGAGCAGG ACGTCCTGGA TGCCTCCAGT GAGGTCAGAT GTCTGGAGGT GGCCCAGCAG 
GCCAATCTCC TTTTCACTGG CCTCGTTTCG GGGGTCGTCC TGGTGTTCCC CCTGAATTCC 
AGACAGGACG TGATGTGCAT CCCCCCTCCC GAGGCCCGGA AAGCCATCAA CTGCATGGCC 
CTCAGTCAAG GAGAGGAGCG CCTGGCCGTC GCCTATGACA GCATCATCCT GGTGCTGGAC 
ATCAGCCCCG GGGATCCCTG TCCGGTTGTC GACGGGCCAA CATACACCTT CTACACCCAG 
CTGCCTGAGA CCATCTCCAG CGTGGCCGTC CTGGCTGACT ACCGTGTGAT CTACGGCATG 
ACCGACGGTG AGCTCTTCCT TTACGAGTGT GCGAATTCCA AAGTGTTCCC TCTGGAGGCC 
CACGGGAGCC GGGTCACCTG TGTGCAGGTC AGCCACAAGG AGCAGCTGGC GGTCAGCGGA 
TCCGAGGAAG CCCTGCTGTG CCTCTGGGAC CTGCAGGCCT GCAAGTGGAG GTTCGAGACG 
AGCTACACGA ATTCTTACTG TAGAGGAGTC CAGTGTGTCT GCTTCTCCAA GGATGACAAG 
TACGTGTATG CGGGCTTGAA GGACCGCTCC ATCATCGTCT GGAGTGTGCT GGATGGCACC 
CTGCTGGCTG TCCAGTTTGT CCACGCCGTG GTGAACAGAA TCATCCCAAC TTCCAGTGGC 
TTCATCGCCC CCACGAGACA CGGCTATCTC ATCCGGGAGC GTTTCCAGGA CCCTTCGTCA 
AGAGCCTCGC AGCAGGACCC TCTCAAGAAC TTCAAGAAGG CAGTGTGGAA GGTCAAATCC 
AGGCAGAGAG ACGAAGCGGC GGCTGTCGCA GGAGGCCCCC AGGACTCGAG ATCAGCAAGT 
GTCCAGGGAA ATGCGTGCAA ATCGAACAAA CGCTCACAAG TCTGCCTGCT CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_011290359.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_011288661.1 Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OFb32181
Clone ID Related Accession (Same CDS sequence)	XM_011290359.2
Accession Version	XM_011290359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018724.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_011290359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCG AGAGAGAAGC CCTACAGAGC ACCGCCTACC CCGAAGTGCA GAACTTCTGC 
CAAAAGCACG GTCTGATGTT TGAGGTTGTT GATCTCAGGT GGGGTATTCG GAACACTGAG 
GCCACTGAGC ACATGACCAC AGAACTCTGC TTGGAGGAAC TCGACCGGTG TCGGAAAACA 
TCCACAGGGC CGGCTTTTGT TGCCCTTCTC GGTGACCAGT ACGGCCCCTG TCCAGTCCCC 
ACGCTGATCG AGGAGAAGGA GTGGGAGGCT CTGAGGGCCC AGCTGACCTC CAAGCCACGC 
GACCTGGAGT TGGTAGCTCA ACACTTCCGG AGGGACGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTGCAGG CCCTGGGCAC CTCGGAGGCC TGTGGGCCCG AAGAGGCCAC GCTGACCTCA 
GTGCTACGCT CTGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CATCGTTATC ACTGGTCAGT CATCGAGTGG GAGATAGACC GGGGCCTGTT GAGCTTGACG 
GATGGAGACC GGGGGGCAAC CATCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCCCAG ATGGCTGCCT GGACACAGAC 
GCCCAGAACC TTCTCCGTGG CCTCAAAGGA CGCATTGCTG ACGTGCAACC GGGAGTTCTC 
AGGGCTCACC ACCTGCCGTG GAGCCGAGAC CTGGTGAACC CTAAAAACAA GGCTCATGCT 
CGCTACCTGA AGGAGCTGGG GGAACAGTTT GTGGCCAGGG CTAGCCACCA GGTCCTGGAG 
CACCTCCGGG AGCTGGAGGC GGGCAGGCAG GAGTTGGCGT GGCTCTATCA GGAGATCCGC 
CACCACCTGG GGCTGAGTGC CGAGGCCAAT CGGACTTTCT GTGGGCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCAGCAGAGC GACAGCCACC CCCACTCTCC CCTGGTGCTC 
TTTGGGCCCC CCGGCATCGG AAAGACAGCT CTGATGTGCA AACTGGCGGA ACAAATGCCA 
GGCCTGCTTG GCCGCAAGAC GGTGACCGTC CTGCGGCTTC TGGGAGCGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCGCCCAGG TGTGCCTGGC CTACGGGTTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CACGCCAGGG TGGTGCAGTT TTTCCACACC 
CTCCTCCACA CCGTCTCCTG CCGCAACTTC GAGTCCCTTG TGATCCTGTT GGATTCCATG 
GACGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CAAATGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCAGGGCGCC AGGGGATTCT AGACACCGTG 
CGACAGACGC TGACGGACCC GGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAAGAGA TGATCCAGCT CCTGCTGGCC GCCTCGAGGA GGACGCTGAG CCCAGGGCAG 
AGGGAACTGC TCTGGGCCAG CCTCCCAGAG TGCGGGCACC CAGGGCGGCT GAGGCTGGCC 
TTCGAGGAGG CCCGGAAATG GGCCTCCTTC ACCATACCTG CCCCCCTGGC CTCCACTGCC 
AAGGAAGCCA TGCACCAACT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GGTCCTGGTG 
GCCCACGTGC TGGGCTACAT CGCGTCTTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGATGA CGAGGTCCTG CAGGTGGTCT ACCGGGACTG GACCCCGCCC 
AGCAAGGAGC TACTGCGTTT TCCACCGCTG CTCTGGGTGA GGCTTCGCCG GGATCTGGGC 
CACTGCTTGG CCCGGCGGCC CGTGGACGGC TTCACGCTCC TGGCCATTGC CCACAGGCAG 
CTGGCCGAGG TGGTCCAAGA ACGCTACCTG CCCGGGGTCG AGAGAGCCAA GAGGCACGGG 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CCAAGAAGCT CGTCACCCTG 
CCGCTCGTGG GGAAGCCCCT GAACCTGGAC CGCATGGTGG CCCCTCAGCC CCTGTGGTTC 
TCGGACACGG TCGCAAATCT GCGAAAGCTG AAGGAGATGC CTCATCACCT GGTCCACTCA 
GGCCGCCTTG AAACGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGC 
GGCATCTCTG GAGGCATCGA GAGCCTGTTG GACGACTTTG ACCTGTATGC CCCTTACCTG 
GACTGTCCGG AGGTCGGCCT GGTCCGGGAA GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTATAT ACAGAACTCC TGGCCAGACT CCATTTCTTC 
ACGACCTCAC ATCCGGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAA CTGGTTCCGG 
ATGTGCCCAC ATCCTGTGCT GGTACCCCTC ACGGGATTCC TCCAGCCCCC GGGAGGACCC 
CTCCGGGCAA CCCTCACCGG CTGTCACAAG GGCATCACGG CCATGGCGTG GAGCGTGGAA 
GAGAGGCTGC TGGTTGTCGG CACCCAGGAT GGCACCGTGG CTGTGTGGGA CACGGAAGAA 
CATCAGGTGA TCCATATGCA CATGGGACAC ACAGGAGAAG TGAGGTATGT GAAGGTGTTT 
GCCAAAGGGA CACTTGCCCT CTCTGCCTCA AAGGACCATA CACTGCGCTT GTGGAATCTA 
CTGACTGGCC AGGAGAAATT CACCATTTGG GATGGAGGCT CAAAGGATCC CATGCAACCT 
GAGACCTGGA GTCTTCACTT GGATGAAACA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGGTCAGTG CGTGGCATCT AGAAACTGCA GAACTGGTTT TCCAGATCCT GGGAGATCCC 
TCTGACTGCT GGGTGTATGC AGGAGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCC 
CAGGGTGGCG TGGCCGGCCT GTGGAGCTCA GCCACAGGAA AGCCGCAGGG GAGGCAACGT 
TTATCCAGCA TCAAGGAAGA AACGCCTACC TGTGGGGTCT CGCTCCAGAA GCAGGGGAAG 
ATGGTCACCG GGTTCAGCAA GGGCTCCGTC TCCTTGATTT CCCCCGAAGG AGACAGCCTG 
CTGGAGAAGC TTCCGGAAGC TGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGAAGATC CGTCCGGATA TTCTTGGCAG ACTCACAGGG GTTTCATCGA 
TTCATGGCCA CCGACCTTGA ACACGAGGAC ACCGTGGAGA CAGCCAGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACCGG GTCCCGGGAC GCCCTCATTC AGGTGTGGAG TCTGTCGGAG 
CAGGGGACCC TGCTGGAAGT TCTGGAAGGC GTCGGGGCCC CCGTGAGCCT GTTAGTCTGG 
GGTGGGGCCT TGGTGGCATC TGCTTCCCAG CAGTCCTCGT CTTTCAAAGT CTGGGATCTC 
ACGTCTGTGC GGCAGCCGAG GGCCTCTACC CCGTTTCTGG ATCGCACTGG CCTCACTGCG 
GTATCACACG ATGGAAGCTA TGTTTACTTC CCCAAGATTG GGGACAAGAA CAAAGTCACC 
ATTTGGGACT TAGCAGAAGG TGAGGAGCAG GACGTCCTGG ATGCCTCCAG TGAGGTCAGA 
TGTCTGGAGG TGGCCCAGCA GGCCAATCTC CTTTTCACTG GCCTCGTTTC GGGGGTCGTC 
CTGGTGTTCC CCCTGAATTC CAGACAGGAC GTGATGTGCA TCCCCCCTCC CGAGGCCCGG 
AAAGCCATCA ACTGCATGGC CCTCAGTCAA GGAGAGGAGC GCCTGGCCGT CGCCTATGAC 
AGCATCATCC TGGTGCTGGA CATCAGCCCC GGGGATCCCT GTCCGGTTGT CGACGGGCCA 
ACATACACCT TCTACACCCA GCTGCCTGAG ACCATCTCCA GCGTGGCCGT CCTGGCTGAC 
TACCGTGTGA TCTACGGCAT GACCGACGGT GAGCTCTTCC TTTACGAGTG TGCGAATTCC 
AAAGTGTTCC CTCTGGAGGC CCACGGGAGC CGGGTCACCT GTGTGCAGGT CAGCCACAAG 
GAGCAGCTGG CGGTCAGCGG ATCCGAGGAA GCCCTGCTGT GCCTCTGGGA CCTGCAGGCC 
TGCAAGTGGA GGTTCGAGAC GAGCTACACG AATTCTTACT GTAGAGGAGT CCAGTGTGTC 
TGCTTCTCCA AGGATGACAA GTACGTGTAT GCGGGCTTGA AGGACCGCTC CATCATCGTC 
TGGAGTGTGC TGGATGGCAC CCTGCTGGCT GTCCAGTTTG TCCACGCCGT GGTGAACAGA 
ATCATCCCAA CTTCCAGTGG CTTCATCGCC CCCACGAGAC ACGGCTATCT CATCCGGGAG 
CGTTTCCAGG ACCCTTCGTC AAGAGCCTCG CAGCAGGACC CTCTCAAGAA CTTCAAGAAG 
GCAGTGTGGA AGGTCAAATC CAGGCAGAGA GACGAAGCGG CGGCTGTCGC AGGAGGCCCC 
CAGGACTCGA GATCAGCAAG TGTCCAGGGA AATGCGTGCA AATCGAACAA ACGCTCACAA 
GTCTGCCTGC TCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011288661.1
CDS	631..5328
Translation	MDAEREALQSTAYPEVQNFCQKHGLMFEVVDLRWGIRNTEATEHMTTELCLEELDRCRKTSTGPAFVALLGDQYGPCPVPTLIEEKEWEALRAQLTSKPRDLELVAQHFRRDENAVPPTYLLQALGTSEACGPEEATLTSVLRSGAQEARRLGLITQEQWHRYHWSVIEWEIDRGLLSLTDGDRGATIFLREIQDLNKHILEDCALKMVDRLPDGCLDTDAQNLLRGLKGRIADVQPGVLRAHHLPWSRDLVNPKNKAHARYLKELGEQFVARASHQVLEHLRELEAGRQELAWLYQEIRHHLGLSAEANRTFCGRQELLAQLGQRLQQSDSHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGASQMSSDARGLLQSICAQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPKCPPRIHLILSACSGRQGILDTVRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAMHQLCARLEQTHGQVLVAHVLGYIASSRHGLSEAELKDVLSLDDEVLQVVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLAEVVQERYLPGVERAKRHGVLADFFSGAWSQGTKKLVTLPLVGKPLNLDRMVAPQPLWFSDTVANLRKLKEMPHHLVHSGRLETLKQEVLGSMNWISCRGISGGIESLLDDFDLYAPYLDCPEVGLVREALQLCRPALELRGMERSILYTELLARLHFFTTSHPALVGQLCQQAQNWFRMCPHPVLVPLTGFLQPPGGPLRATLTGCHKGITAMAWSVEERLLVVGTQDGTVAVWDTEEHQVIHMHMGHTGEVRYVKVFAKGTLALSASKDHTLRLWNLLTGQEKFTIWDGGSKDPMQPETWSLHLDETKKVVYLASGSKVSAWHLETAELVFQILGDPSDCWVYAGVLASRATLLTVSQGGVAGLWSSATGKPQGRQRLSSIKEETPTCGVSLQKQGKMVTGFSKGSVSLISPEGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVETASFGPENNLIVTGSRDALIQVWSLSEQGTLLEVLEGVGAPVSLLVWGGALVASASQQSSSFKVWDLTSVRQPRASTPFLDRTGLTAVSHDGSYVYFPKIGDKNKVTIWDLAEGEEQDVLDASSEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSQGEERLAVAYDSIILVLDISPGDPCPVVDGPTYTFYTQLPETISSVAVLADYRVIYGMTDGELFLYECANSKVFPLEAHGSRVTCVQVSHKEQLAVSGSEEALLCLWDLQACKWRFETSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSSGFIAPTRHGYLIRERFQDPSSRASQQDPLKNFKKAVWKVKSRQRDEAAAVAGGPQDSRSASVQGNACKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_011290359.2
Organism	Felis catus(Domestic cat)
Definition	Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011290359.2

ORF Insert Sequence:

ATGGATGCCG AGAGAGAAGC CCTACAGAGC ACCGCCTACC CCGAAGTGCA GAACTTCTGC 
CAAAAGCACG GTCTGATGTT TGAGGTTGTT GATCTCAGGT GGGGTATTCG GAACACTGAG 
GCCACTGAGC ACATGACCAC AGAACTCTGC TTGGAGGAAC TCGACCGGTG TCGGAAAACA 
TCCACAGGGC CGGCTTTTGT TGCCCTTCTC GGTGACCAGT ACGGCCCCTG TCCAGTCCCC 
ACGCTGATCG AGGAGAAGGA GTGGGAGGCT CTGAGGGCCC AGCTGACCTC CAAGCCACGC 
GACCTGGAGT TGGTAGCTCA ACACTTCCGG AGGGACGAGA ACGCCGTGCC CCCCACCTAC 
CTGCTGCAGG CCCTGGGCAC CTCGGAGGCC TGTGGGCCCG AAGAGGCCAC GCTGACCTCA 
GTGCTACGCT CTGGAGCCCA GGAGGCCCGG AGGCTGGGCC TCATCACCCA GGAGCAGTGG 
CATCGTTATC ACTGGTCAGT CATCGAGTGG GAGATAGACC GGGGCCTGTT GAGCTTGACG 
GATGGAGACC GGGGGGCAAC CATCTTCTTG AGAGAGATCC AAGACCTCAA CAAACACATC 
CTGGAAGACT GCGCCCTCAA GATGGTGGAC CGGCTCCCAG ATGGCTGCCT GGACACAGAC 
GCCCAGAACC TTCTCCGTGG CCTCAAAGGA CGCATTGCTG ACGTGCAACC GGGAGTTCTC 
AGGGCTCACC ACCTGCCGTG GAGCCGAGAC CTGGTGAACC CTAAAAACAA GGCTCATGCT 
CGCTACCTGA AGGAGCTGGG GGAACAGTTT GTGGCCAGGG CTAGCCACCA GGTCCTGGAG 
CACCTCCGGG AGCTGGAGGC GGGCAGGCAG GAGTTGGCGT GGCTCTATCA GGAGATCCGC 
CACCACCTGG GGCTGAGTGC CGAGGCCAAT CGGACTTTCT GTGGGCGCCA GGAGCTCCTG 
GCCCAGCTGG GGCAGCGGCT CCAGCAGAGC GACAGCCACC CCCACTCTCC CCTGGTGCTC 
TTTGGGCCCC CCGGCATCGG AAAGACAGCT CTGATGTGCA AACTGGCGGA ACAAATGCCA 
GGCCTGCTTG GCCGCAAGAC GGTGACCGTC CTGCGGCTTC TGGGAGCGTC ACAGATGAGC 
TCCGACGCCC GAGGCCTGCT CCAGAGCATC TGCGCCCAGG TGTGCCTGGC CTACGGGTTG 
CCCCTGCCCC CTGCCCAGGT CCTGGAGGCC CACGCCAGGG TGGTGCAGTT TTTCCACACC 
CTCCTCCACA CCGTCTCCTG CCGCAACTTC GAGTCCCTTG TGATCCTGTT GGATTCCATG 
GACGATGTGG ATTCCATCTG CCGCGCCCGG AGGGTCCCCT GGCTGCCTCC CAAATGCCCC 
CCGAGGATCC ACCTTATCCT CTCAGCCTGC TCAGGGCGCC AGGGGATTCT AGACACCGTG 
CGACAGACGC TGACGGACCC GGAGGCCTAC TGGGAGGTGA AACCCCTCTC CGGAAACCAA 
GGGCAAGAGA TGATCCAGCT CCTGCTGGCC GCCTCGAGGA GGACGCTGAG CCCAGGGCAG 
AGGGAACTGC TCTGGGCCAG CCTCCCAGAG TGCGGGCACC CAGGGCGGCT GAGGCTGGCC 
TTCGAGGAGG CCCGGAAATG GGCCTCCTTC ACCATACCTG CCCCCCTGGC CTCCACTGCC 
AAGGAAGCCA TGCACCAACT GTGTGCCCGC CTGGAGCAGA CACACGGGCA GGTCCTGGTG 
GCCCACGTGC TGGGCTACAT CGCGTCTTCC CGGCATGGGC TCTCAGAGGC AGAGCTGAAG 
GACGTCCTGT CCTTGGATGA CGAGGTCCTG CAGGTGGTCT ACCGGGACTG GACCCCGCCC 
AGCAAGGAGC TACTGCGTTT TCCACCGCTG CTCTGGGTGA GGCTTCGCCG GGATCTGGGC 
CACTGCTTGG CCCGGCGGCC CGTGGACGGC TTCACGCTCC TGGCCATTGC CCACAGGCAG 
CTGGCCGAGG TGGTCCAAGA ACGCTACCTG CCCGGGGTCG AGAGAGCCAA GAGGCACGGG 
GTCTTGGCTG ACTTCTTCTC AGGGGCCTGG AGCCAGGGCA CCAAGAAGCT CGTCACCCTG 
CCGCTCGTGG GGAAGCCCCT GAACCTGGAC CGCATGGTGG CCCCTCAGCC CCTGTGGTTC 
TCGGACACGG TCGCAAATCT GCGAAAGCTG AAGGAGATGC CTCATCACCT GGTCCACTCA 
GGCCGCCTTG AAACGCTGAA GCAGGAGGTT CTGGGCAGCA TGAACTGGAT TTCCTGCCGC 
GGCATCTCTG GAGGCATCGA GAGCCTGTTG GACGACTTTG ACCTGTATGC CCCTTACCTG 
GACTGTCCGG AGGTCGGCCT GGTCCGGGAA GCCCTCCAGC TGTGCCGCCC GGCCCTGGAG 
CTCCGAGGCA TGGAGAGGAG CATCCTATAT ACAGAACTCC TGGCCAGACT CCATTTCTTC 
ACGACCTCAC ATCCGGCGCT GGTGGGACAG CTGTGCCAAC AGGCCCAGAA CTGGTTCCGG 
ATGTGCCCAC ATCCTGTGCT GGTACCCCTC ACGGGATTCC TCCAGCCCCC GGGAGGACCC 
CTCCGGGCAA CCCTCACCGG CTGTCACAAG GGCATCACGG CCATGGCGTG GAGCGTGGAA 
GAGAGGCTGC TGGTTGTCGG CACCCAGGAT GGCACCGTGG CTGTGTGGGA CACGGAAGAA 
CATCAGGTGA TCCATATGCA CATGGGACAC ACAGGAGAAG TGAGGTATGT GAAGGTGTTT 
GCCAAAGGGA CACTTGCCCT CTCTGCCTCA AAGGACCATA CACTGCGCTT GTGGAATCTA 
CTGACTGGCC AGGAGAAATT CACCATTTGG GATGGAGGCT CAAAGGATCC CATGCAACCT 
GAGACCTGGA GTCTTCACTT GGATGAAACA AAGAAGGTTG TGTATTTGGC ATCTGGCTCA 
AAGGTCAGTG CGTGGCATCT AGAAACTGCA GAACTGGTTT TCCAGATCCT GGGAGATCCC 
TCTGACTGCT GGGTGTATGC AGGAGTGCTG GCTTCCCGGG CCACGCTGCT GACCGTGTCC 
CAGGGTGGCG TGGCCGGCCT GTGGAGCTCA GCCACAGGAA AGCCGCAGGG GAGGCAACGT 
TTATCCAGCA TCAAGGAAGA AACGCCTACC TGTGGGGTCT CGCTCCAGAA GCAGGGGAAG 
ATGGTCACCG GGTTCAGCAA GGGCTCCGTC TCCTTGATTT CCCCCGAAGG AGACAGCCTG 
CTGGAGAAGC TTCCGGAAGC TGTGAGGTTC CTGGTAGTCT CTGAAGATGA GTCCCTTCTC 
GCTGCAGGGT TTGGAAGATC CGTCCGGATA TTCTTGGCAG ACTCACAGGG GTTTCATCGA 
TTCATGGCCA CCGACCTTGA ACACGAGGAC ACCGTGGAGA CAGCCAGTTT TGGTCCTGAG 
AACAATCTGA TTGTCACCGG GTCCCGGGAC GCCCTCATTC AGGTGTGGAG TCTGTCGGAG 
CAGGGGACCC TGCTGGAAGT TCTGGAAGGC GTCGGGGCCC CCGTGAGCCT GTTAGTCTGG 
GGTGGGGCCT TGGTGGCATC TGCTTCCCAG CAGTCCTCGT CTTTCAAAGT CTGGGATCTC 
ACGTCTGTGC GGCAGCCGAG GGCCTCTACC CCGTTTCTGG ATCGCACTGG CCTCACTGCG 
GTATCACACG ATGGAAGCTA TGTTTACTTC CCCAAGATTG GGGACAAGAA CAAAGTCACC 
ATTTGGGACT TAGCAGAAGG TGAGGAGCAG GACGTCCTGG ATGCCTCCAG TGAGGTCAGA 
TGTCTGGAGG TGGCCCAGCA GGCCAATCTC CTTTTCACTG GCCTCGTTTC GGGGGTCGTC 
CTGGTGTTCC CCCTGAATTC CAGACAGGAC GTGATGTGCA TCCCCCCTCC CGAGGCCCGG 
AAAGCCATCA ACTGCATGGC CCTCAGTCAA GGAGAGGAGC GCCTGGCCGT CGCCTATGAC 
AGCATCATCC TGGTGCTGGA CATCAGCCCC GGGGATCCCT GTCCGGTTGT CGACGGGCCA 
ACATACACCT TCTACACCCA GCTGCCTGAG ACCATCTCCA GCGTGGCCGT CCTGGCTGAC 
TACCGTGTGA TCTACGGCAT GACCGACGGT GAGCTCTTCC TTTACGAGTG TGCGAATTCC 
AAAGTGTTCC CTCTGGAGGC CCACGGGAGC CGGGTCACCT GTGTGCAGGT CAGCCACAAG 
GAGCAGCTGG CGGTCAGCGG ATCCGAGGAA GCCCTGCTGT GCCTCTGGGA CCTGCAGGCC 
TGCAAGTGGA GGTTCGAGAC GAGCTACACG AATTCTTACT GTAGAGGAGT CCAGTGTGTC 
TGCTTCTCCA AGGATGACAA GTACGTGTAT GCGGGCTTGA AGGACCGCTC CATCATCGTC 
TGGAGTGTGC TGGATGGCAC CCTGCTGGCT GTCCAGTTTG TCCACGCCGT GGTGAACAGA 
ATCATCCCAA CTTCCAGTGG CTTCATCGCC CCCACGAGAC ACGGCTATCT CATCCGGGAG 
CGTTTCCAGG ACCCTTCGTC AAGAGCCTCG CAGCAGGACC CTCTCAAGAA CTTCAAGAAG 
GCAGTGTGGA AGGTCAAATC CAGGCAGAGA GACGAAGCGG CGGCTGTCGC AGGAGGCCCC 
CAGGACTCGA GATCAGCAAG TGTCCAGGGA AATGCGTGCA AATCGAACAA ACGCTCACAA 
GTCTGCCTGC TCGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_019819124.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019674683.1 Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.

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CloneID	OFb38247
Clone ID Related Accession (Same CDS sequence)	XM_019819124.1 , XM_019819120.1
Accession Version	XM_019819124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018724.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCGGC CAGTGTCCCA GGAAACAGGC CACTTGCTTA ACTTCTCCAG GGCAGCAATG 
GACGGACAAG CATTCCAACC AGACCCATGG ACTCCCAGCT TTATTGACAG ATCATCAGCG 
TGTATAACAC CGTATATGGA TGCCGAGAGA GAAGCCCTAC AGAGCACCGC CTACCCCGAA 
GTGCAGAACT TCTGCCAAAA GCACGGTCTG ATGTTTGAGG TTGTTGATCT CAGGTGGGGT 
ATTCGGAACA CTGAGGCCAC TGAGCACATG ACCACAGAAC TCTGCTTGGA GGAACTCGAC 
CGGTGTCGGA AAACATCCAC AGGGCCGGCT TTTGTTGCCC TTCTCGGTGA CCAGTACGGC 
CCCTGTCCAG TCCCCACGCT GATCGAGGAG AAGGAGTGGG AGGCTCTGAG GGCCCAGCTG 
ACCTCCAAGC CACGCGACCT GGAGTTGGTA GCTCAACACT TCCGGAGGGA CGAGAACGCC 
GTGCCCCCCA CCTACCTGCT GCAGGCCCTG GGCACCTCGG AGGCCTGTGG GCCCGAAGAG 
GCCACGCTGA CCTCAGTGCT ACGCTCTGGA GCCCAGGAGG CCCGGAGGCT GGGCCTCATC 
ACCCAGGAGC AGTGGCATCG TTATCACTGG TCAGTCATCG AGTGGGAGAT AGACCGGGGC 
CTGTTGAGCT TGACGGATGG AGACCGGGGG GCAACCATCT TCTTGAGAGA GATCCAAGAC 
CTCAACAAAC ACATCCTGGA AGACTGCGCC CTCAAGATGG TGGACCGGCT CCCAGATGGC 
TGCCTGGACA CAGACGCCCA GAACCTTCTC CGTGGCCTCA AAGGACGCAT TGCTGACGTG 
CAACCGGGAG TTCTCAGGGC TCACCACCTG CCGTGGAGCC GAGACCTGGT GAACCCTAAA 
AACAAGGCTC ATGCTCGCTA CCTGAAGGAG CTGGGGGAAC AGTTTGTGGC CAGGGCTAGC 
CACCAGGTCC TGGAGCACCT CCGGGAGCTG GAGGCGGGCA GGCAGGAGTT GGCGTGGCTC 
TATCAGGAGA TCCGCCACCA CCTGGGGCTG AGTGCCGAGG CCAATCGGAC TTTCTGTGGG 
CGCCAGGAGC TCCTGGCCCA GCTGGGGCAG CGGCTCCAGC AGAGCGACAG CCACCCCCAC 
TCTCCCCTGG TGCTCTTTGG GCCCCCCGGC ATCGGAAAGA CAGCTCTGAT GTGCAAACTG 
GCGGAACAAA TGCCAGGCCT GCTTGGCCGC AAGACGGTGA CCGTCCTGCG GCTTCTGGGA 
GCGTCACAGA TGAGCTCCGA CGCCCGAGGC CTGCTCCAGA GCATCTGCGC CCAGGTGTGC 
CTGGCCTACG GGTTGCCCCT GCCCCCTGCC CAGGTCCTGG AGGCCCACGC CAGGGTGGTG 
CAGTTTTTCC ACACCCTCCT CCACACCGTC TCCTGCCGCA ACTTCGAGTC CCTTGTGATC 
CTGTTGGATT CCATGGACGA TGTGGATTCC ATCTGCCGCG CCCGGAGGGT CCCCTGGCTG 
CCTCCCAAAT GCCCCCCGAG GATCCACCTT ATCCTCTCAG CCTGCTCAGG GCGCCAGGGG 
ATTCTAGACA CCGTGCGACA GACGCTGACG GACCCGGAGG CCTACTGGGA GGTGAAACCC 
CTCTCCGGAA ACCAAGGGCA AGAGATGATC CAGCTCCTGC TGGCCGCCTC GAGGAGGACG 
CTGAGCCCAG GGCAGAGGGA ACTGCTCTGG GCCAGCCTCC CAGAGTGCGG GCACCCAGGG 
CGGCTGAGGC TGGCCTTCGA GGAGGCCCGG AAATGGGCCT CCTTCACCAT ACCTGCCCCC 
CTGGCCTCCA CTGCCAAGGA AGCCATGCAC CAACTGTGTG CCCGCCTGGA GCAGACACAC 
GGGCAGGTCC TGGTGGCCCA CGTGCTGGGC TACATCGCGT CTTCCCGGCA TGGGCTCTCA 
GAGGCAGAGC TGAAGGACGT CCTGTCCTTG GATGACGAGG TCCTGCAGGT GGTCTACCGG 
GACTGGACCC CGCCCAGCAA GGAGCTACTG CGTTTTCCAC CGCTGCTCTG GGTGAGGCTT 
CGCCGGGATC TGGGCCACTG CTTGGCCCGG CGGCCCGTGG ACGGCTTCAC GCTCCTGGCC 
ATTGCCCACA GGCAGCTGGC CGAGGTGGTC CAAGAACGCT ACCTGCCCGG GGTCGAGAGA 
GCCAAGAGGC ACGGGGTCTT GGCTGACTTC TTCTCAGGGG CCTGGAGCCA GGGCACCAAG 
AAGCTCGTCA CCCTGCCGCT CGTGGGGAAG CCCCTGAACC TGGACCGCAT GGTGGCCCCT 
CAGCCCCTGT GGTTCTCGGA CACGGTCGCA AATCTGCGAA AGCTGAAGGA GATGCCTCAT 
CACCTGGTCC ACTCAGGCCG CCTTGAAACG CTGAAGCAGG AGGTTCTGGG CAGCATGAAC 
TGGATTTCCT GCCGCGGCAT CTCTGGAGGC ATCGAGAGCC TGTTGGACGA CTTTGACCTG 
TATGCCCCTT ACCTGGACTG TCCGGAGGTC GGCCTGGTCC GGGAAGCCCT CCAGCTGTGC 
CGCCCGGCCC TGGAGCTCCG AGGCATGGAG AGGAGCATCC TATATACAGA ACTCCTGGCC 
AGACTCCATT TCTTCACGAC CTCACATCCG GCGCTGGTGG GACAGCTGTG CCAACAGGCC 
CAGAACTGGT TCCGGATGTG CCCACATCCT GTGCTGGTAC CCCTCACGGG ATTCCTCCAG 
CCCCCGGGAG GACCCCTCCG GGCAACCCTC ACCGGCTGTC ACAAGGGCAT CACGGCCATG 
GCGTGGAGCG TGGAAGAGAG GCTGCTGGTT GTCGGCACCC AGGATGGCAC CGTGGCTGTG 
TGGGACACGG AAGAACATCA GGTGATCCAT ATGCACATGG GACACACAGG AGAAGTGAGG 
TATGTGAAGG TGTTTGCCAA AGGGACACTT GCCCTCTCTG CCTCAAAGGA CCATACACTG 
CGCTTGTGGA ATCTACTGAC TGGCCAGGAG AAATTCACCA TTTGGGATGG AGGCTCAAAG 
GATCCCATGC AACCTGAGAC CTGGAGTCTT CACTTGGATG AAACAAAGAA GGTTGTGTAT 
TTGGCATCTG GCTCAAAGGT CAGTGCGTGG CATCTAGAAA CTGCAGAACT GGTTTTCCAG 
ATCCTGGGAG ATCCCTCTGA CTGCTGGGTG TATGCAGGAG TGCTGGCTTC CCGGGCCACG 
CTGCTGACCG TGTCCCAGGG TGGCGTGGCC GGCCTGTGGA GCTCAGCCAC AGGAAAGCCG 
CAGGGGAGGC AACGTTTATC CAGCATCAAG GAAGAAACGC CTACCTGTGG GGTCTCGCTC 
CAGAAGCAGG GGAAGATGGT CACCGGGTTC AGCAAGGGCT CCGTCTCCTT GATTTCCCCC 
GAAGGAGACA GCCTGCTGGA GAAGCTTCCG GAAGCTGTGA GGTTCCTGGT AGTCTCTGAA 
GATGAGTCCC TTCTCGCTGC AGGGTTTGGA AGATCCGTCC GGATATTCTT GGCAGACTCA 
CAGGGGTTTC ATCGATTCAT GGCCACCGAC CTTGAACACG AGGACACCGT GGAGACAGCC 
AGTTTTGGTC CTGAGAACAA TCTGATTGTC ACCGGGTCCC GGGACGCCCT CATTCAGGTG 
TGGAGTCTGT CGGAGCAGGG GACCCTGCTG GAAGTTCTGG AAGGCGTCGG GGCCCCCGTG 
AGCCTGTTAG TCTGGGGTGG GGCCTTGGTG GCATCTGCTT CCCAGCAGTC CTCGTCTTTC 
AAAGTCTGGG ATCTCACGTC TGTGCGGCAG CCGAGGGCCT CTACCCCGTT TCTGGATCGC 
ACTGGCCTCA CTGCGGTATC ACACGATGGA AGCTATGTTT ACTTCCCCAA GATTGGGGAC 
AAGAACAAAG TCACCATTTG GGACTTAGCA GAAGGTGAGG AGCAGGACGT CCTGGATGCC 
TCCAGTGAGG TCAGATGTCT GGAGGTGGCC CAGCAGGCCA ATCTCCTTTT CACTGGCCTC 
GTTTCGGGGG TCGTCCTGGT GTTCCCCCTG AATTCCAGAC AGGACGTGAT GTGCATCCCC 
CCTCCCGAGG CCCGGAAAGC CATCAACTGC ATGGCCCTCA GTCAAGGAGA GGAGCGCCTG 
GCCGTCGCCT ATGACAGCAT CATCCTGGTG CTGGACATCA GCCCCGGGGA TCCCTGTCCG 
GTTGTCGACG GGCCAACATA CACCTTCTAC ACCCAGCTGC CTGAGACCAT CTCCAGCGTG 
GCCGTCCTGG CTGACTACCG TGTGATCTAC GGCATGACCG ACGGTGAGCT CTTCCTTTAC 
GAGTGTGCGA ATTCCAAAGT GTTCCCTCTG GAGGCCCACG GGAGCCGGGT CACCTGTGTG 
CAGGTCAGCC ACAAGGAGCA GCTGGCGGTC AGCGGATCCG AGGAAGCCCT GCTGTGCCTC 
TGGGACCTGC AGGCCTGCAA GTGGAGGTTC GAGACGAGCT ACACGAATTC TTACTGTAGA 
GGAGTCCAGT GTGTCTGCTT CTCCAAGGAT GACAAGTACG TGTATGCGGG CTTGAAGGAC 
CGCTCCATCA TCGTCTGGAG TGTGCTGGAT GGCACCCTGC TGGCTGTCCA GTTTGTCCAC 
GCCGTGGTGA ACAGAATCAT CCCAACTTCC AGTGGCTTCA TCGCCCCCAC GAGACACGGC 
TATCTCATCC GGGAGCGTTT CCAGGACCCT TCGTCAAGAG CCTCGCAGCA GGACCCTCTC 
AAGAACTTCA AGAAGGCAGT GTGGAAGGTC AAATCCAGGC AGAGAGACGA AGCGGCGGCT 
GTCGCAGGAG GCCCCCAGGA CTCGAGATCA GCAAGTGTCC AGGGAAATGC GTGCAAATCG 
AACAAACGCT CACAAGTCTG CCTGCTCGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019674683.1
CDS	212..5044
Translation	MVRPVSQETGHLLNFSRAAMDGQAFQPDPWTPSFIDRSSACITPYMDAEREALQSTAYPEVQNFCQKHGLMFEVVDLRWGIRNTEATEHMTTELCLEELDRCRKTSTGPAFVALLGDQYGPCPVPTLIEEKEWEALRAQLTSKPRDLELVAQHFRRDENAVPPTYLLQALGTSEACGPEEATLTSVLRSGAQEARRLGLITQEQWHRYHWSVIEWEIDRGLLSLTDGDRGATIFLREIQDLNKHILEDCALKMVDRLPDGCLDTDAQNLLRGLKGRIADVQPGVLRAHHLPWSRDLVNPKNKAHARYLKELGEQFVARASHQVLEHLRELEAGRQELAWLYQEIRHHLGLSAEANRTFCGRQELLAQLGQRLQQSDSHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGASQMSSDARGLLQSICAQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPKCPPRIHLILSACSGRQGILDTVRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAMHQLCARLEQTHGQVLVAHVLGYIASSRHGLSEAELKDVLSLDDEVLQVVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLAEVVQERYLPGVERAKRHGVLADFFSGAWSQGTKKLVTLPLVGKPLNLDRMVAPQPLWFSDTVANLRKLKEMPHHLVHSGRLETLKQEVLGSMNWISCRGISGGIESLLDDFDLYAPYLDCPEVGLVREALQLCRPALELRGMERSILYTELLARLHFFTTSHPALVGQLCQQAQNWFRMCPHPVLVPLTGFLQPPGGPLRATLTGCHKGITAMAWSVEERLLVVGTQDGTVAVWDTEEHQVIHMHMGHTGEVRYVKVFAKGTLALSASKDHTLRLWNLLTGQEKFTIWDGGSKDPMQPETWSLHLDETKKVVYLASGSKVSAWHLETAELVFQILGDPSDCWVYAGVLASRATLLTVSQGGVAGLWSSATGKPQGRQRLSSIKEETPTCGVSLQKQGKMVTGFSKGSVSLISPEGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVETASFGPENNLIVTGSRDALIQVWSLSEQGTLLEVLEGVGAPVSLLVWGGALVASASQQSSSFKVWDLTSVRQPRASTPFLDRTGLTAVSHDGSYVYFPKIGDKNKVTIWDLAEGEEQDVLDASSEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSQGEERLAVAYDSIILVLDISPGDPCPVVDGPTYTFYTQLPETISSVAVLADYRVIYGMTDGELFLYECANSKVFPLEAHGSRVTCVQVSHKEQLAVSGSEEALLCLWDLQACKWRFETSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSSGFIAPTRHGYLIRERFQDPSSRASQQDPLKNFKKAVWKVKSRQRDEAAAVAGGPQDSRSASVQGNACKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_019819124.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019819124.1

ORF Insert Sequence:

ATGGTGCGGC CAGTGTCCCA GGAAACAGGC CACTTGCTTA ACTTCTCCAG GGCAGCAATG 
GACGGACAAG CATTCCAACC AGACCCATGG ACTCCCAGCT TTATTGACAG ATCATCAGCG 
TGTATAACAC CGTATATGGA TGCCGAGAGA GAAGCCCTAC AGAGCACCGC CTACCCCGAA 
GTGCAGAACT TCTGCCAAAA GCACGGTCTG ATGTTTGAGG TTGTTGATCT CAGGTGGGGT 
ATTCGGAACA CTGAGGCCAC TGAGCACATG ACCACAGAAC TCTGCTTGGA GGAACTCGAC 
CGGTGTCGGA AAACATCCAC AGGGCCGGCT TTTGTTGCCC TTCTCGGTGA CCAGTACGGC 
CCCTGTCCAG TCCCCACGCT GATCGAGGAG AAGGAGTGGG AGGCTCTGAG GGCCCAGCTG 
ACCTCCAAGC CACGCGACCT GGAGTTGGTA GCTCAACACT TCCGGAGGGA CGAGAACGCC 
GTGCCCCCCA CCTACCTGCT GCAGGCCCTG GGCACCTCGG AGGCCTGTGG GCCCGAAGAG 
GCCACGCTGA CCTCAGTGCT ACGCTCTGGA GCCCAGGAGG CCCGGAGGCT GGGCCTCATC 
ACCCAGGAGC AGTGGCATCG TTATCACTGG TCAGTCATCG AGTGGGAGAT AGACCGGGGC 
CTGTTGAGCT TGACGGATGG AGACCGGGGG GCAACCATCT TCTTGAGAGA GATCCAAGAC 
CTCAACAAAC ACATCCTGGA AGACTGCGCC CTCAAGATGG TGGACCGGCT CCCAGATGGC 
TGCCTGGACA CAGACGCCCA GAACCTTCTC CGTGGCCTCA AAGGACGCAT TGCTGACGTG 
CAACCGGGAG TTCTCAGGGC TCACCACCTG CCGTGGAGCC GAGACCTGGT GAACCCTAAA 
AACAAGGCTC ATGCTCGCTA CCTGAAGGAG CTGGGGGAAC AGTTTGTGGC CAGGGCTAGC 
CACCAGGTCC TGGAGCACCT CCGGGAGCTG GAGGCGGGCA GGCAGGAGTT GGCGTGGCTC 
TATCAGGAGA TCCGCCACCA CCTGGGGCTG AGTGCCGAGG CCAATCGGAC TTTCTGTGGG 
CGCCAGGAGC TCCTGGCCCA GCTGGGGCAG CGGCTCCAGC AGAGCGACAG CCACCCCCAC 
TCTCCCCTGG TGCTCTTTGG GCCCCCCGGC ATCGGAAAGA CAGCTCTGAT GTGCAAACTG 
GCGGAACAAA TGCCAGGCCT GCTTGGCCGC AAGACGGTGA CCGTCCTGCG GCTTCTGGGA 
GCGTCACAGA TGAGCTCCGA CGCCCGAGGC CTGCTCCAGA GCATCTGCGC CCAGGTGTGC 
CTGGCCTACG GGTTGCCCCT GCCCCCTGCC CAGGTCCTGG AGGCCCACGC CAGGGTGGTG 
CAGTTTTTCC ACACCCTCCT CCACACCGTC TCCTGCCGCA ACTTCGAGTC CCTTGTGATC 
CTGTTGGATT CCATGGACGA TGTGGATTCC ATCTGCCGCG CCCGGAGGGT CCCCTGGCTG 
CCTCCCAAAT GCCCCCCGAG GATCCACCTT ATCCTCTCAG CCTGCTCAGG GCGCCAGGGG 
ATTCTAGACA CCGTGCGACA GACGCTGACG GACCCGGAGG CCTACTGGGA GGTGAAACCC 
CTCTCCGGAA ACCAAGGGCA AGAGATGATC CAGCTCCTGC TGGCCGCCTC GAGGAGGACG 
CTGAGCCCAG GGCAGAGGGA ACTGCTCTGG GCCAGCCTCC CAGAGTGCGG GCACCCAGGG 
CGGCTGAGGC TGGCCTTCGA GGAGGCCCGG AAATGGGCCT CCTTCACCAT ACCTGCCCCC 
CTGGCCTCCA CTGCCAAGGA AGCCATGCAC CAACTGTGTG CCCGCCTGGA GCAGACACAC 
GGGCAGGTCC TGGTGGCCCA CGTGCTGGGC TACATCGCGT CTTCCCGGCA TGGGCTCTCA 
GAGGCAGAGC TGAAGGACGT CCTGTCCTTG GATGACGAGG TCCTGCAGGT GGTCTACCGG 
GACTGGACCC CGCCCAGCAA GGAGCTACTG CGTTTTCCAC CGCTGCTCTG GGTGAGGCTT 
CGCCGGGATC TGGGCCACTG CTTGGCCCGG CGGCCCGTGG ACGGCTTCAC GCTCCTGGCC 
ATTGCCCACA GGCAGCTGGC CGAGGTGGTC CAAGAACGCT ACCTGCCCGG GGTCGAGAGA 
GCCAAGAGGC ACGGGGTCTT GGCTGACTTC TTCTCAGGGG CCTGGAGCCA GGGCACCAAG 
AAGCTCGTCA CCCTGCCGCT CGTGGGGAAG CCCCTGAACC TGGACCGCAT GGTGGCCCCT 
CAGCCCCTGT GGTTCTCGGA CACGGTCGCA AATCTGCGAA AGCTGAAGGA GATGCCTCAT 
CACCTGGTCC ACTCAGGCCG CCTTGAAACG CTGAAGCAGG AGGTTCTGGG CAGCATGAAC 
TGGATTTCCT GCCGCGGCAT CTCTGGAGGC ATCGAGAGCC TGTTGGACGA CTTTGACCTG 
TATGCCCCTT ACCTGGACTG TCCGGAGGTC GGCCTGGTCC GGGAAGCCCT CCAGCTGTGC 
CGCCCGGCCC TGGAGCTCCG AGGCATGGAG AGGAGCATCC TATATACAGA ACTCCTGGCC 
AGACTCCATT TCTTCACGAC CTCACATCCG GCGCTGGTGG GACAGCTGTG CCAACAGGCC 
CAGAACTGGT TCCGGATGTG CCCACATCCT GTGCTGGTAC CCCTCACGGG ATTCCTCCAG 
CCCCCGGGAG GACCCCTCCG GGCAACCCTC ACCGGCTGTC ACAAGGGCAT CACGGCCATG 
GCGTGGAGCG TGGAAGAGAG GCTGCTGGTT GTCGGCACCC AGGATGGCAC CGTGGCTGTG 
TGGGACACGG AAGAACATCA GGTGATCCAT ATGCACATGG GACACACAGG AGAAGTGAGG 
TATGTGAAGG TGTTTGCCAA AGGGACACTT GCCCTCTCTG CCTCAAAGGA CCATACACTG 
CGCTTGTGGA ATCTACTGAC TGGCCAGGAG AAATTCACCA TTTGGGATGG AGGCTCAAAG 
GATCCCATGC AACCTGAGAC CTGGAGTCTT CACTTGGATG AAACAAAGAA GGTTGTGTAT 
TTGGCATCTG GCTCAAAGGT CAGTGCGTGG CATCTAGAAA CTGCAGAACT GGTTTTCCAG 
ATCCTGGGAG ATCCCTCTGA CTGCTGGGTG TATGCAGGAG TGCTGGCTTC CCGGGCCACG 
CTGCTGACCG TGTCCCAGGG TGGCGTGGCC GGCCTGTGGA GCTCAGCCAC AGGAAAGCCG 
CAGGGGAGGC AACGTTTATC CAGCATCAAG GAAGAAACGC CTACCTGTGG GGTCTCGCTC 
CAGAAGCAGG GGAAGATGGT CACCGGGTTC AGCAAGGGCT CCGTCTCCTT GATTTCCCCC 
GAAGGAGACA GCCTGCTGGA GAAGCTTCCG GAAGCTGTGA GGTTCCTGGT AGTCTCTGAA 
GATGAGTCCC TTCTCGCTGC AGGGTTTGGA AGATCCGTCC GGATATTCTT GGCAGACTCA 
CAGGGGTTTC ATCGATTCAT GGCCACCGAC CTTGAACACG AGGACACCGT GGAGACAGCC 
AGTTTTGGTC CTGAGAACAA TCTGATTGTC ACCGGGTCCC GGGACGCCCT CATTCAGGTG 
TGGAGTCTGT CGGAGCAGGG GACCCTGCTG GAAGTTCTGG AAGGCGTCGG GGCCCCCGTG 
AGCCTGTTAG TCTGGGGTGG GGCCTTGGTG GCATCTGCTT CCCAGCAGTC CTCGTCTTTC 
AAAGTCTGGG ATCTCACGTC TGTGCGGCAG CCGAGGGCCT CTACCCCGTT TCTGGATCGC 
ACTGGCCTCA CTGCGGTATC ACACGATGGA AGCTATGTTT ACTTCCCCAA GATTGGGGAC 
AAGAACAAAG TCACCATTTG GGACTTAGCA GAAGGTGAGG AGCAGGACGT CCTGGATGCC 
TCCAGTGAGG TCAGATGTCT GGAGGTGGCC CAGCAGGCCA ATCTCCTTTT CACTGGCCTC 
GTTTCGGGGG TCGTCCTGGT GTTCCCCCTG AATTCCAGAC AGGACGTGAT GTGCATCCCC 
CCTCCCGAGG CCCGGAAAGC CATCAACTGC ATGGCCCTCA GTCAAGGAGA GGAGCGCCTG 
GCCGTCGCCT ATGACAGCAT CATCCTGGTG CTGGACATCA GCCCCGGGGA TCCCTGTCCG 
GTTGTCGACG GGCCAACATA CACCTTCTAC ACCCAGCTGC CTGAGACCAT CTCCAGCGTG 
GCCGTCCTGG CTGACTACCG TGTGATCTAC GGCATGACCG ACGGTGAGCT CTTCCTTTAC 
GAGTGTGCGA ATTCCAAAGT GTTCCCTCTG GAGGCCCACG GGAGCCGGGT CACCTGTGTG 
CAGGTCAGCC ACAAGGAGCA GCTGGCGGTC AGCGGATCCG AGGAAGCCCT GCTGTGCCTC 
TGGGACCTGC AGGCCTGCAA GTGGAGGTTC GAGACGAGCT ACACGAATTC TTACTGTAGA 
GGAGTCCAGT GTGTCTGCTT CTCCAAGGAT GACAAGTACG TGTATGCGGG CTTGAAGGAC 
CGCTCCATCA TCGTCTGGAG TGTGCTGGAT GGCACCCTGC TGGCTGTCCA GTTTGTCCAC 
GCCGTGGTGA ACAGAATCAT CCCAACTTCC AGTGGCTTCA TCGCCCCCAC GAGACACGGC 
TATCTCATCC GGGAGCGTTT CCAGGACCCT TCGTCAAGAG CCTCGCAGCA GGACCCTCTC 
AAGAACTTCA AGAAGGCAGT GTGGAAGGTC AAATCCAGGC AGAGAGACGA AGCGGCGGCT 
GTCGCAGGAG GCCCCCAGGA CTCGAGATCA GCAAGTGTCC AGGGAAATGC GTGCAAATCG 
AACAAACGCT CACAAGTCTG CCTGCTCGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_019819120.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019674679.1 Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

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CloneID	OFb38247
Clone ID Related Accession (Same CDS sequence)	XM_019819124.1 , XM_019819120.1
Accession Version	XM_019819120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018724.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCGGC CAGTGTCCCA GGAAACAGGC CACTTGCTTA ACTTCTCCAG GGCAGCAATG 
GACGGACAAG CATTCCAACC AGACCCATGG ACTCCCAGCT TTATTGACAG ATCATCAGCG 
TGTATAACAC CGTATATGGA TGCCGAGAGA GAAGCCCTAC AGAGCACCGC CTACCCCGAA 
GTGCAGAACT TCTGCCAAAA GCACGGTCTG ATGTTTGAGG TTGTTGATCT CAGGTGGGGT 
ATTCGGAACA CTGAGGCCAC TGAGCACATG ACCACAGAAC TCTGCTTGGA GGAACTCGAC 
CGGTGTCGGA AAACATCCAC AGGGCCGGCT TTTGTTGCCC TTCTCGGTGA CCAGTACGGC 
CCCTGTCCAG TCCCCACGCT GATCGAGGAG AAGGAGTGGG AGGCTCTGAG GGCCCAGCTG 
ACCTCCAAGC CACGCGACCT GGAGTTGGTA GCTCAACACT TCCGGAGGGA CGAGAACGCC 
GTGCCCCCCA CCTACCTGCT GCAGGCCCTG GGCACCTCGG AGGCCTGTGG GCCCGAAGAG 
GCCACGCTGA CCTCAGTGCT ACGCTCTGGA GCCCAGGAGG CCCGGAGGCT GGGCCTCATC 
ACCCAGGAGC AGTGGCATCG TTATCACTGG TCAGTCATCG AGTGGGAGAT AGACCGGGGC 
CTGTTGAGCT TGACGGATGG AGACCGGGGG GCAACCATCT TCTTGAGAGA GATCCAAGAC 
CTCAACAAAC ACATCCTGGA AGACTGCGCC CTCAAGATGG TGGACCGGCT CCCAGATGGC 
TGCCTGGACA CAGACGCCCA GAACCTTCTC CGTGGCCTCA AAGGACGCAT TGCTGACGTG 
CAACCGGGAG TTCTCAGGGC TCACCACCTG CCGTGGAGCC GAGACCTGGT GAACCCTAAA 
AACAAGGCTC ATGCTCGCTA CCTGAAGGAG CTGGGGGAAC AGTTTGTGGC CAGGGCTAGC 
CACCAGGTCC TGGAGCACCT CCGGGAGCTG GAGGCGGGCA GGCAGGAGTT GGCGTGGCTC 
TATCAGGAGA TCCGCCACCA CCTGGGGCTG AGTGCCGAGG CCAATCGGAC TTTCTGTGGG 
CGCCAGGAGC TCCTGGCCCA GCTGGGGCAG CGGCTCCAGC AGAGCGACAG CCACCCCCAC 
TCTCCCCTGG TGCTCTTTGG GCCCCCCGGC ATCGGAAAGA CAGCTCTGAT GTGCAAACTG 
GCGGAACAAA TGCCAGGCCT GCTTGGCCGC AAGACGGTGA CCGTCCTGCG GCTTCTGGGA 
GCGTCACAGA TGAGCTCCGA CGCCCGAGGC CTGCTCCAGA GCATCTGCGC CCAGGTGTGC 
CTGGCCTACG GGTTGCCCCT GCCCCCTGCC CAGGTCCTGG AGGCCCACGC CAGGGTGGTG 
CAGTTTTTCC ACACCCTCCT CCACACCGTC TCCTGCCGCA ACTTCGAGTC CCTTGTGATC 
CTGTTGGATT CCATGGACGA TGTGGATTCC ATCTGCCGCG CCCGGAGGGT CCCCTGGCTG 
CCTCCCAAAT GCCCCCCGAG GATCCACCTT ATCCTCTCAG CCTGCTCAGG GCGCCAGGGG 
ATTCTAGACA CCGTGCGACA GACGCTGACG GACCCGGAGG CCTACTGGGA GGTGAAACCC 
CTCTCCGGAA ACCAAGGGCA AGAGATGATC CAGCTCCTGC TGGCCGCCTC GAGGAGGACG 
CTGAGCCCAG GGCAGAGGGA ACTGCTCTGG GCCAGCCTCC CAGAGTGCGG GCACCCAGGG 
CGGCTGAGGC TGGCCTTCGA GGAGGCCCGG AAATGGGCCT CCTTCACCAT ACCTGCCCCC 
CTGGCCTCCA CTGCCAAGGA AGCCATGCAC CAACTGTGTG CCCGCCTGGA GCAGACACAC 
GGGCAGGTCC TGGTGGCCCA CGTGCTGGGC TACATCGCGT CTTCCCGGCA TGGGCTCTCA 
GAGGCAGAGC TGAAGGACGT CCTGTCCTTG GATGACGAGG TCCTGCAGGT GGTCTACCGG 
GACTGGACCC CGCCCAGCAA GGAGCTACTG CGTTTTCCAC CGCTGCTCTG GGTGAGGCTT 
CGCCGGGATC TGGGCCACTG CTTGGCCCGG CGGCCCGTGG ACGGCTTCAC GCTCCTGGCC 
ATTGCCCACA GGCAGCTGGC CGAGGTGGTC CAAGAACGCT ACCTGCCCGG GGTCGAGAGA 
GCCAAGAGGC ACGGGGTCTT GGCTGACTTC TTCTCAGGGG CCTGGAGCCA GGGCACCAAG 
AAGCTCGTCA CCCTGCCGCT CGTGGGGAAG CCCCTGAACC TGGACCGCAT GGTGGCCCCT 
CAGCCCCTGT GGTTCTCGGA CACGGTCGCA AATCTGCGAA AGCTGAAGGA GATGCCTCAT 
CACCTGGTCC ACTCAGGCCG CCTTGAAACG CTGAAGCAGG AGGTTCTGGG CAGCATGAAC 
TGGATTTCCT GCCGCGGCAT CTCTGGAGGC ATCGAGAGCC TGTTGGACGA CTTTGACCTG 
TATGCCCCTT ACCTGGACTG TCCGGAGGTC GGCCTGGTCC GGGAAGCCCT CCAGCTGTGC 
CGCCCGGCCC TGGAGCTCCG AGGCATGGAG AGGAGCATCC TATATACAGA ACTCCTGGCC 
AGACTCCATT TCTTCACGAC CTCACATCCG GCGCTGGTGG GACAGCTGTG CCAACAGGCC 
CAGAACTGGT TCCGGATGTG CCCACATCCT GTGCTGGTAC CCCTCACGGG ATTCCTCCAG 
CCCCCGGGAG GACCCCTCCG GGCAACCCTC ACCGGCTGTC ACAAGGGCAT CACGGCCATG 
GCGTGGAGCG TGGAAGAGAG GCTGCTGGTT GTCGGCACCC AGGATGGCAC CGTGGCTGTG 
TGGGACACGG AAGAACATCA GGTGATCCAT ATGCACATGG GACACACAGG AGAAGTGAGG 
TATGTGAAGG TGTTTGCCAA AGGGACACTT GCCCTCTCTG CCTCAAAGGA CCATACACTG 
CGCTTGTGGA ATCTACTGAC TGGCCAGGAG AAATTCACCA TTTGGGATGG AGGCTCAAAG 
GATCCCATGC AACCTGAGAC CTGGAGTCTT CACTTGGATG AAACAAAGAA GGTTGTGTAT 
TTGGCATCTG GCTCAAAGGT CAGTGCGTGG CATCTAGAAA CTGCAGAACT GGTTTTCCAG 
ATCCTGGGAG ATCCCTCTGA CTGCTGGGTG TATGCAGGAG TGCTGGCTTC CCGGGCCACG 
CTGCTGACCG TGTCCCAGGG TGGCGTGGCC GGCCTGTGGA GCTCAGCCAC AGGAAAGCCG 
CAGGGGAGGC AACGTTTATC CAGCATCAAG GAAGAAACGC CTACCTGTGG GGTCTCGCTC 
CAGAAGCAGG GGAAGATGGT CACCGGGTTC AGCAAGGGCT CCGTCTCCTT GATTTCCCCC 
GAAGGAGACA GCCTGCTGGA GAAGCTTCCG GAAGCTGTGA GGTTCCTGGT AGTCTCTGAA 
GATGAGTCCC TTCTCGCTGC AGGGTTTGGA AGATCCGTCC GGATATTCTT GGCAGACTCA 
CAGGGGTTTC ATCGATTCAT GGCCACCGAC CTTGAACACG AGGACACCGT GGAGACAGCC 
AGTTTTGGTC CTGAGAACAA TCTGATTGTC ACCGGGTCCC GGGACGCCCT CATTCAGGTG 
TGGAGTCTGT CGGAGCAGGG GACCCTGCTG GAAGTTCTGG AAGGCGTCGG GGCCCCCGTG 
AGCCTGTTAG TCTGGGGTGG GGCCTTGGTG GCATCTGCTT CCCAGCAGTC CTCGTCTTTC 
AAAGTCTGGG ATCTCACGTC TGTGCGGCAG CCGAGGGCCT CTACCCCGTT TCTGGATCGC 
ACTGGCCTCA CTGCGGTATC ACACGATGGA AGCTATGTTT ACTTCCCCAA GATTGGGGAC 
AAGAACAAAG TCACCATTTG GGACTTAGCA GAAGGTGAGG AGCAGGACGT CCTGGATGCC 
TCCAGTGAGG TCAGATGTCT GGAGGTGGCC CAGCAGGCCA ATCTCCTTTT CACTGGCCTC 
GTTTCGGGGG TCGTCCTGGT GTTCCCCCTG AATTCCAGAC AGGACGTGAT GTGCATCCCC 
CCTCCCGAGG CCCGGAAAGC CATCAACTGC ATGGCCCTCA GTCAAGGAGA GGAGCGCCTG 
GCCGTCGCCT ATGACAGCAT CATCCTGGTG CTGGACATCA GCCCCGGGGA TCCCTGTCCG 
GTTGTCGACG GGCCAACATA CACCTTCTAC ACCCAGCTGC CTGAGACCAT CTCCAGCGTG 
GCCGTCCTGG CTGACTACCG TGTGATCTAC GGCATGACCG ACGGTGAGCT CTTCCTTTAC 
GAGTGTGCGA ATTCCAAAGT GTTCCCTCTG GAGGCCCACG GGAGCCGGGT CACCTGTGTG 
CAGGTCAGCC ACAAGGAGCA GCTGGCGGTC AGCGGATCCG AGGAAGCCCT GCTGTGCCTC 
TGGGACCTGC AGGCCTGCAA GTGGAGGTTC GAGACGAGCT ACACGAATTC TTACTGTAGA 
GGAGTCCAGT GTGTCTGCTT CTCCAAGGAT GACAAGTACG TGTATGCGGG CTTGAAGGAC 
CGCTCCATCA TCGTCTGGAG TGTGCTGGAT GGCACCCTGC TGGCTGTCCA GTTTGTCCAC 
GCCGTGGTGA ACAGAATCAT CCCAACTTCC AGTGGCTTCA TCGCCCCCAC GAGACACGGC 
TATCTCATCC GGGAGCGTTT CCAGGACCCT TCGTCAAGAG CCTCGCAGCA GGACCCTCTC 
AAGAACTTCA AGAAGGCAGT GTGGAAGGTC AAATCCAGGC AGAGAGACGA AGCGGCGGCT 
GTCGCAGGAG GCCCCCAGGA CTCGAGATCA GCAAGTGTCC AGGGAAATGC GTGCAAATCG 
AACAAACGCT CACAAGTCTG CCTGCTCGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019674679.1
CDS	533..5365
Translation	MVRPVSQETGHLLNFSRAAMDGQAFQPDPWTPSFIDRSSACITPYMDAEREALQSTAYPEVQNFCQKHGLMFEVVDLRWGIRNTEATEHMTTELCLEELDRCRKTSTGPAFVALLGDQYGPCPVPTLIEEKEWEALRAQLTSKPRDLELVAQHFRRDENAVPPTYLLQALGTSEACGPEEATLTSVLRSGAQEARRLGLITQEQWHRYHWSVIEWEIDRGLLSLTDGDRGATIFLREIQDLNKHILEDCALKMVDRLPDGCLDTDAQNLLRGLKGRIADVQPGVLRAHHLPWSRDLVNPKNKAHARYLKELGEQFVARASHQVLEHLRELEAGRQELAWLYQEIRHHLGLSAEANRTFCGRQELLAQLGQRLQQSDSHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGASQMSSDARGLLQSICAQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPKCPPRIHLILSACSGRQGILDTVRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAMHQLCARLEQTHGQVLVAHVLGYIASSRHGLSEAELKDVLSLDDEVLQVVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLAEVVQERYLPGVERAKRHGVLADFFSGAWSQGTKKLVTLPLVGKPLNLDRMVAPQPLWFSDTVANLRKLKEMPHHLVHSGRLETLKQEVLGSMNWISCRGISGGIESLLDDFDLYAPYLDCPEVGLVREALQLCRPALELRGMERSILYTELLARLHFFTTSHPALVGQLCQQAQNWFRMCPHPVLVPLTGFLQPPGGPLRATLTGCHKGITAMAWSVEERLLVVGTQDGTVAVWDTEEHQVIHMHMGHTGEVRYVKVFAKGTLALSASKDHTLRLWNLLTGQEKFTIWDGGSKDPMQPETWSLHLDETKKVVYLASGSKVSAWHLETAELVFQILGDPSDCWVYAGVLASRATLLTVSQGGVAGLWSSATGKPQGRQRLSSIKEETPTCGVSLQKQGKMVTGFSKGSVSLISPEGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVETASFGPENNLIVTGSRDALIQVWSLSEQGTLLEVLEGVGAPVSLLVWGGALVASASQQSSSFKVWDLTSVRQPRASTPFLDRTGLTAVSHDGSYVYFPKIGDKNKVTIWDLAEGEEQDVLDASSEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSQGEERLAVAYDSIILVLDISPGDPCPVVDGPTYTFYTQLPETISSVAVLADYRVIYGMTDGELFLYECANSKVFPLEAHGSRVTCVQVSHKEQLAVSGSEEALLCLWDLQACKWRFETSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSSGFIAPTRHGYLIRERFQDPSSRASQQDPLKNFKKAVWKVKSRQRDEAAAVAGGPQDSRSASVQGNACKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_019819120.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019819120.1

ORF Insert Sequence:

ATGGTGCGGC CAGTGTCCCA GGAAACAGGC CACTTGCTTA ACTTCTCCAG GGCAGCAATG 
GACGGACAAG CATTCCAACC AGACCCATGG ACTCCCAGCT TTATTGACAG ATCATCAGCG 
TGTATAACAC CGTATATGGA TGCCGAGAGA GAAGCCCTAC AGAGCACCGC CTACCCCGAA 
GTGCAGAACT TCTGCCAAAA GCACGGTCTG ATGTTTGAGG TTGTTGATCT CAGGTGGGGT 
ATTCGGAACA CTGAGGCCAC TGAGCACATG ACCACAGAAC TCTGCTTGGA GGAACTCGAC 
CGGTGTCGGA AAACATCCAC AGGGCCGGCT TTTGTTGCCC TTCTCGGTGA CCAGTACGGC 
CCCTGTCCAG TCCCCACGCT GATCGAGGAG AAGGAGTGGG AGGCTCTGAG GGCCCAGCTG 
ACCTCCAAGC CACGCGACCT GGAGTTGGTA GCTCAACACT TCCGGAGGGA CGAGAACGCC 
GTGCCCCCCA CCTACCTGCT GCAGGCCCTG GGCACCTCGG AGGCCTGTGG GCCCGAAGAG 
GCCACGCTGA CCTCAGTGCT ACGCTCTGGA GCCCAGGAGG CCCGGAGGCT GGGCCTCATC 
ACCCAGGAGC AGTGGCATCG TTATCACTGG TCAGTCATCG AGTGGGAGAT AGACCGGGGC 
CTGTTGAGCT TGACGGATGG AGACCGGGGG GCAACCATCT TCTTGAGAGA GATCCAAGAC 
CTCAACAAAC ACATCCTGGA AGACTGCGCC CTCAAGATGG TGGACCGGCT CCCAGATGGC 
TGCCTGGACA CAGACGCCCA GAACCTTCTC CGTGGCCTCA AAGGACGCAT TGCTGACGTG 
CAACCGGGAG TTCTCAGGGC TCACCACCTG CCGTGGAGCC GAGACCTGGT GAACCCTAAA 
AACAAGGCTC ATGCTCGCTA CCTGAAGGAG CTGGGGGAAC AGTTTGTGGC CAGGGCTAGC 
CACCAGGTCC TGGAGCACCT CCGGGAGCTG GAGGCGGGCA GGCAGGAGTT GGCGTGGCTC 
TATCAGGAGA TCCGCCACCA CCTGGGGCTG AGTGCCGAGG CCAATCGGAC TTTCTGTGGG 
CGCCAGGAGC TCCTGGCCCA GCTGGGGCAG CGGCTCCAGC AGAGCGACAG CCACCCCCAC 
TCTCCCCTGG TGCTCTTTGG GCCCCCCGGC ATCGGAAAGA CAGCTCTGAT GTGCAAACTG 
GCGGAACAAA TGCCAGGCCT GCTTGGCCGC AAGACGGTGA CCGTCCTGCG GCTTCTGGGA 
GCGTCACAGA TGAGCTCCGA CGCCCGAGGC CTGCTCCAGA GCATCTGCGC CCAGGTGTGC 
CTGGCCTACG GGTTGCCCCT GCCCCCTGCC CAGGTCCTGG AGGCCCACGC CAGGGTGGTG 
CAGTTTTTCC ACACCCTCCT CCACACCGTC TCCTGCCGCA ACTTCGAGTC CCTTGTGATC 
CTGTTGGATT CCATGGACGA TGTGGATTCC ATCTGCCGCG CCCGGAGGGT CCCCTGGCTG 
CCTCCCAAAT GCCCCCCGAG GATCCACCTT ATCCTCTCAG CCTGCTCAGG GCGCCAGGGG 
ATTCTAGACA CCGTGCGACA GACGCTGACG GACCCGGAGG CCTACTGGGA GGTGAAACCC 
CTCTCCGGAA ACCAAGGGCA AGAGATGATC CAGCTCCTGC TGGCCGCCTC GAGGAGGACG 
CTGAGCCCAG GGCAGAGGGA ACTGCTCTGG GCCAGCCTCC CAGAGTGCGG GCACCCAGGG 
CGGCTGAGGC TGGCCTTCGA GGAGGCCCGG AAATGGGCCT CCTTCACCAT ACCTGCCCCC 
CTGGCCTCCA CTGCCAAGGA AGCCATGCAC CAACTGTGTG CCCGCCTGGA GCAGACACAC 
GGGCAGGTCC TGGTGGCCCA CGTGCTGGGC TACATCGCGT CTTCCCGGCA TGGGCTCTCA 
GAGGCAGAGC TGAAGGACGT CCTGTCCTTG GATGACGAGG TCCTGCAGGT GGTCTACCGG 
GACTGGACCC CGCCCAGCAA GGAGCTACTG CGTTTTCCAC CGCTGCTCTG GGTGAGGCTT 
CGCCGGGATC TGGGCCACTG CTTGGCCCGG CGGCCCGTGG ACGGCTTCAC GCTCCTGGCC 
ATTGCCCACA GGCAGCTGGC CGAGGTGGTC CAAGAACGCT ACCTGCCCGG GGTCGAGAGA 
GCCAAGAGGC ACGGGGTCTT GGCTGACTTC TTCTCAGGGG CCTGGAGCCA GGGCACCAAG 
AAGCTCGTCA CCCTGCCGCT CGTGGGGAAG CCCCTGAACC TGGACCGCAT GGTGGCCCCT 
CAGCCCCTGT GGTTCTCGGA CACGGTCGCA AATCTGCGAA AGCTGAAGGA GATGCCTCAT 
CACCTGGTCC ACTCAGGCCG CCTTGAAACG CTGAAGCAGG AGGTTCTGGG CAGCATGAAC 
TGGATTTCCT GCCGCGGCAT CTCTGGAGGC ATCGAGAGCC TGTTGGACGA CTTTGACCTG 
TATGCCCCTT ACCTGGACTG TCCGGAGGTC GGCCTGGTCC GGGAAGCCCT CCAGCTGTGC 
CGCCCGGCCC TGGAGCTCCG AGGCATGGAG AGGAGCATCC TATATACAGA ACTCCTGGCC 
AGACTCCATT TCTTCACGAC CTCACATCCG GCGCTGGTGG GACAGCTGTG CCAACAGGCC 
CAGAACTGGT TCCGGATGTG CCCACATCCT GTGCTGGTAC CCCTCACGGG ATTCCTCCAG 
CCCCCGGGAG GACCCCTCCG GGCAACCCTC ACCGGCTGTC ACAAGGGCAT CACGGCCATG 
GCGTGGAGCG TGGAAGAGAG GCTGCTGGTT GTCGGCACCC AGGATGGCAC CGTGGCTGTG 
TGGGACACGG AAGAACATCA GGTGATCCAT ATGCACATGG GACACACAGG AGAAGTGAGG 
TATGTGAAGG TGTTTGCCAA AGGGACACTT GCCCTCTCTG CCTCAAAGGA CCATACACTG 
CGCTTGTGGA ATCTACTGAC TGGCCAGGAG AAATTCACCA TTTGGGATGG AGGCTCAAAG 
GATCCCATGC AACCTGAGAC CTGGAGTCTT CACTTGGATG AAACAAAGAA GGTTGTGTAT 
TTGGCATCTG GCTCAAAGGT CAGTGCGTGG CATCTAGAAA CTGCAGAACT GGTTTTCCAG 
ATCCTGGGAG ATCCCTCTGA CTGCTGGGTG TATGCAGGAG TGCTGGCTTC CCGGGCCACG 
CTGCTGACCG TGTCCCAGGG TGGCGTGGCC GGCCTGTGGA GCTCAGCCAC AGGAAAGCCG 
CAGGGGAGGC AACGTTTATC CAGCATCAAG GAAGAAACGC CTACCTGTGG GGTCTCGCTC 
CAGAAGCAGG GGAAGATGGT CACCGGGTTC AGCAAGGGCT CCGTCTCCTT GATTTCCCCC 
GAAGGAGACA GCCTGCTGGA GAAGCTTCCG GAAGCTGTGA GGTTCCTGGT AGTCTCTGAA 
GATGAGTCCC TTCTCGCTGC AGGGTTTGGA AGATCCGTCC GGATATTCTT GGCAGACTCA 
CAGGGGTTTC ATCGATTCAT GGCCACCGAC CTTGAACACG AGGACACCGT GGAGACAGCC 
AGTTTTGGTC CTGAGAACAA TCTGATTGTC ACCGGGTCCC GGGACGCCCT CATTCAGGTG 
TGGAGTCTGT CGGAGCAGGG GACCCTGCTG GAAGTTCTGG AAGGCGTCGG GGCCCCCGTG 
AGCCTGTTAG TCTGGGGTGG GGCCTTGGTG GCATCTGCTT CCCAGCAGTC CTCGTCTTTC 
AAAGTCTGGG ATCTCACGTC TGTGCGGCAG CCGAGGGCCT CTACCCCGTT TCTGGATCGC 
ACTGGCCTCA CTGCGGTATC ACACGATGGA AGCTATGTTT ACTTCCCCAA GATTGGGGAC 
AAGAACAAAG TCACCATTTG GGACTTAGCA GAAGGTGAGG AGCAGGACGT CCTGGATGCC 
TCCAGTGAGG TCAGATGTCT GGAGGTGGCC CAGCAGGCCA ATCTCCTTTT CACTGGCCTC 
GTTTCGGGGG TCGTCCTGGT GTTCCCCCTG AATTCCAGAC AGGACGTGAT GTGCATCCCC 
CCTCCCGAGG CCCGGAAAGC CATCAACTGC ATGGCCCTCA GTCAAGGAGA GGAGCGCCTG 
GCCGTCGCCT ATGACAGCAT CATCCTGGTG CTGGACATCA GCCCCGGGGA TCCCTGTCCG 
GTTGTCGACG GGCCAACATA CACCTTCTAC ACCCAGCTGC CTGAGACCAT CTCCAGCGTG 
GCCGTCCTGG CTGACTACCG TGTGATCTAC GGCATGACCG ACGGTGAGCT CTTCCTTTAC 
GAGTGTGCGA ATTCCAAAGT GTTCCCTCTG GAGGCCCACG GGAGCCGGGT CACCTGTGTG 
CAGGTCAGCC ACAAGGAGCA GCTGGCGGTC AGCGGATCCG AGGAAGCCCT GCTGTGCCTC 
TGGGACCTGC AGGCCTGCAA GTGGAGGTTC GAGACGAGCT ACACGAATTC TTACTGTAGA 
GGAGTCCAGT GTGTCTGCTT CTCCAAGGAT GACAAGTACG TGTATGCGGG CTTGAAGGAC 
CGCTCCATCA TCGTCTGGAG TGTGCTGGAT GGCACCCTGC TGGCTGTCCA GTTTGTCCAC 
GCCGTGGTGA ACAGAATCAT CCCAACTTCC AGTGGCTTCA TCGCCCCCAC GAGACACGGC 
TATCTCATCC GGGAGCGTTT CCAGGACCCT TCGTCAAGAG CCTCGCAGCA GGACCCTCTC 
AAGAACTTCA AGAAGGCAGT GTGGAAGGTC AAATCCAGGC AGAGAGACGA AGCGGCGGCT 
GTCGCAGGAG GCCCCCAGGA CTCGAGATCA GCAAGTGTCC AGGGAAATGC GTGCAAATCG 
AACAAACGCT CACAAGTCTG CCTGCTCGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_019819129.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019674688.1 Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X4, mRNA.

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CloneID	OFb38248
Clone ID Related Accession (Same CDS sequence)	XM_019819129.1
Accession Version	XM_019819129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	NACHT domain- and WD repeat-containing protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018724.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCGGC CAGTGTCCCA GGAAACAGGC CACTTGCTTA ACTTCTCCAG GGCAGCAATG 
GACGGACAAG CATTCCAACC AGACCCATGG ACTCCCAGCT TTATTGACAG ATCATCAGCG 
TGTATAACAC CGTATATGGA TGCCGAGAGA GAAGCCCTAC AGAGCACCGC CTACCCCGAA 
GTGCAGAACT TCTGCCAAAA GCACGGTCTG ATGTTTGAGG TTGTTGATCT CAGGTGGGGT 
ATTCGGAACA CTGAGGCCAC TGAGCACATG ACCACAGAAC TCTGCTTGGA GGAACTCGAC 
CGGTGTCGGA AAACATCCAC AGGGCCGGCT TTTGTTGCCC TTCTCGGTGA CCAGTACGGC 
CCCTGTCCAG TCCCCACGCT GATCGAGGAG AAGGAGTGGG AGGCTCTGAG GGCCCAGCTG 
ACCTCCAAGC CACGCGACCT GGAGTTGGTA GCTCAACACT TCCGGAGGGA CGAGAACGCC 
GTGCCCCCCA CCTACCTGCT GCAGGCCCTG GGCACCTCGG AGGCCTGTGG GCCCGAAGAG 
GCCACGCTGA CCTCAGTGCT ACGCTCTGGA GCCCAGGAGG CCCGGAGGCT GGGCCTCATC 
ACCCAGGAGC AGTGGCATCG TTATCACTGG TCAGTCATCG AGTGGGAGAT AGACCGGGGC 
CTGTTGAGCT TGACGGATGG AGACCGGGGG GCAACCATCT TCTTGAGAGA GATCCAAGAC 
CTCAACAAAC ACATCCTGGA AGACTGCGCC CTCAAGATGG TGGACCGGCT CCCAGATGGC 
TGCCTGGACA CAGACGCCCA GAACCTTCTC CGTGGCCTCA AAGGACGCAT TGCTGACGTG 
CAACCGGGAG TTCTCAGGGC TCACCACCTG CCGTGGAGCC GAGACCTGGT GAACCCTAAA 
AACAAGGCTC ATGCTCGCTA CCTGAAGGAG CTGGGGGAAC AGTTTGTGGC CAGGGCTAGC 
CACCAGGTCC TGGAGCACCT CCGGGAGCTG GAGGCGGGCA GGCAGGAGTT GGCGTGGCTC 
TATCAGGAGA TCCGCCACCA CCTGGGGCTG AGTGCCGAGG CCAATCGGAC TTTCTGTGGG 
CGCCAGGAGC TCCTGGCCCA GCTGGGGCAG CGGCTCCAGC AGAGCGACAG CCACCCCCAC 
TCTCCCCTGG TGCTCTTTGG GCCCCCCGGC ATCGGAAAGA CAGCTCTGAT GTGCAAACTG 
GCGGAACAAA TGCCAGGCCT GCTTGGCCGC AAGACGGTGA CCGTCCTGCG GCTTCTGGGA 
GCGTCACAGA TGAGCTCCGA CGCCCGAGGC CTGCTCCAGA GCATCTGCGC CCAGGTGTGC 
CTGGCCTACG GGTTGCCCCT GCCCCCTGCC CAGGTCCTGG AGGCCCACGC CAGGGTGGTG 
CAGTTTTTCC ACACCCTCCT CCACACCGTC TCCTGCCGCA ACTTCGAGTC CCTTGTGATC 
CTGTTGGATT CCATGGACGA TGTGGATTCC ATCTGCCGCG CCCGGAGGGT CCCCTGGCTG 
CCTCCCAAAT GCCCCCCGAG GATCCACCTT ATCCTCTCAG CCTGCTCAGG GCGCCAGGGG 
ATTCTAGACA CCGTGCGACA GACGCTGACG GACCCGGAGG CCTACTGGGA GGTGAAACCC 
CTCTCCGGAA ACCAAGGGCA AGAGATGATC CAGCTCCTGC TGGCCGCCTC GAGGAGGACG 
CTGAGCCCAG GGCAGAGGGA ACTGCTCTGG GCCAGCCTCC CAGAGTGCGG GCACCCAGGG 
CGGCTGAGGC TGGCCTTCGA GGAGGCCCGG AAATGGGCCT CCTTCACCAT ACCTGCCCCC 
CTGGCCTCCA CTGCCAAGGA AGCCATGCAC CAACTGTGTG CCCGCCTGGA GCAGACACAC 
GGGCAGGTCC TGGTGGCCCA CGTGCTGGGC TACATCGCGT CTTCCCGGCA TGGGCTCTCA 
GAGGCAGAGC TGAAGGACGT CCTGTCCTTG GATGACGAGG TCCTGCAGGT GGTCTACCGG 
GACTGGACCC CGCCCAGCAA GGAGCTACTG CGTTTTCCAC CGCTGCTCTG GGTGAGGCTT 
CGCCGGGATC TGGGCCACTG CTTGGCCCGG CGGCCCGTGG ACGGCTTCAC GCTCCTGGCC 
ATTGCCCACA GGCAGCTGGC CGAGGTGGTC CAAGAACGCT ACCTGCCCGG GGTCGAGAGA 
GCCAAGAGGC ACGGGGTCTT GGCTGACTTC TTCTCAGGGG CCTGGAGCCA GGGCACCAAG 
AAGCTCGTCA CCCTGCCGCT CGTGGGGAAG CCCCTGAACC TGGACCGCAT GGTGGCCCCT 
CAGCCCCTGT GGTTCTCGGA CACGGTCGCA AATCTGCGAA AGCTGAAGGA GATGCCTCAT 
CACCTGGTCC ACTCAGGCCG CCTTGAAACG CTGAAGCAGG AGGTTCTGGG CAGCATGAAC 
TGGATTTCCT GCCGCGGCAT CTCTGGAGGC ATCGAGAGCC TGTTGGACGA CTTTGACCTG 
TATGCCCCTT ACCTGGACTG TCCGGAGGTC GGCCTGGTCC GGGAAGCCCT CCAGCTGTGC 
CGCCCGGCCC TGGAGCTCCG AGGCATGGAG AGGAGCATCC TATATACAGA ACTCCTGGCC 
AGACTCCATT TCTTCACGAC CTCACATCCG GCGCTGGTGG GACAGCTGTG CCAACAGGCC 
CAGAACTGGT TCCGGATGTG CCCACATCCT GTGCTGGTAC CCCTCACGGG ATTCCTCCAG 
CCCCCGGGAG GACCCCTCCG GGCAACCCTC ACCGGCTGTC ACAAGGGCAT CACGGCCATG 
GCGTGGAGCG TGGAAGAGAG GCTGCTGGTT GTCGGCACCC AGGATGGCAC CGTGGCTGTG 
TGGGACACGG AAGAACATCA GGTGATCCAT ATGCACATGG GACACACAGG AGAAGTGAGG 
TATGTGAAGG TGTTTGCCAA AGGGACACTT GCCCTCTCTG CCTCAAAGGA CCATACACTG 
CGCTTGTGGA ATCTACTGAC TGGCCAGGAG AAATTCACCA TTTGGGATGG AGGCTCAAAG 
GATCCCATGC AACCTGAGAC CTGGAGTCTT CACTTGGATG AAACAAAGAA GGTTGTGTAT 
TTGGCATCTG GCTCAAAGGT CAGTGCGTGG CATCTAGAAA CTGCAGAACT GGTTTTCCAG 
ATCCTGGGAG ATCCCTCTGA CTGCTGGGTG TATGCAGGAG TGCTGGCTTC CCGGGCCACG 
CTGCTGACCG TGTCCCAGGG TGGCGTGGCC GGCCTGTGGA GCTCAGCCAC AGGAAAGCCG 
CAGGGGAGGC AACGTTTATC CAGCATCAAG GAAGAAACGC CTACCTGTGG GGTCTCGCTC 
CAGAAGCAGG GGAAGATGGT CACCGGGTTC AGCAAGGGCT CCGTCTCCTT GATTTCCCCC 
GAAGGAGACA GCCTGCTGGA GAAGCTTCCG GAAGCTGTGA GGTTCCTGGT AGTCTCTGAA 
GATGAGTCCC TTCTCGCTGC AGGGTTTGGA AGATCCGTCC GGATATTCTT GGCAGACTCA 
CAGGGGTTTC ATCGATTCAT GGCCACCGAC CTTGAACACG AGGACACCGT GGAGACAGCC 
AGTTTTGGTC CTGAGAACAA TCTGATTGTC ACCGGGTCCC GGGACGCCCT CATTCAGGTG 
TGGAGTCTGT CGGAGCAGGG GACCCTGCTG GAAGTTCTGG AAGGCGTCGG GGCCCCCGTG 
AGCCTGTTAG TCTGGGGTGG GGCCTTGGTG GCATCTGCTT CCCAGCAGTC CTCGTCTTTC 
AAAGTCTGGG ATCTCACGTC TGTGCGGCAG CCGAGGGCCT CTACCCCGTT TCTGGATCGC 
ACTGGCCTCA CTGCGGTATC ACACGATGGA AGCTATGTTT ACTTCCCCAA GATTGGGGAC 
AAGAACAAAG TCACCATTTG GGACTTAGCA GAAGGTGAGG AGCAGGACGT CCTGGATGCC 
TCCAGTGAGG TCAGATGTCT GGAGGTGGCC CAGCAGGCCA ATCTCCTTTT CACTGGCCTC 
GTTTCGGGGG TCGTCCTGGT GTTCCCCCTG AATTCCAGAC AGGACGTGAT GTGCATCCCC 
CCTCCCGAGG CCCGGAAAGC CATCAACTGC ATGGCCCTCA GTCAAGGAGA GGAGCGCCTG 
GCCGTCGCCT ATGACAGCAT CATCCTGGTG CTGGACATCA GCCCCGGGGA TCCCTGTCCG 
GTTGTCGACG GGCCAACATA CACCTTCTAC ACCCAGCTGC CTGAGACCAT CTCCAGCGTG 
GCCGTCCTGG CTGACTACCG TGTGATCTAC GGCATGACCG ACGGTGAGCT CTTCCTTTAC 
GAGTGTGCGA ATTCCAAAGT GTTCCCTCTG GAGGCCCACG GGAGCCGGGT CACCTGTGTG 
CAGGTCAGCC ACAAGGAGCA GCTGGCGGTC AGCGGATCCG AGGAAGCCCT GCTGTGCCTC 
TGGGACCTGC AGGCCTGCAA GTGGAGGTTC GAGACGAGCT ACACGAATTC TTACTGTAGA 
GGAGTCCAGT GTGTCTGCTT CTCCAAGGAT GACAAGTACG TGTATGCGGG CTTGAAGGAC 
CGCTCCATCA TCGTCTGGAG TGTGCTGGAT GCTCCCTGGA GCTCCTTTCT GTGTGCTAGA 
CAGGATACCG CCCAATTCTT AAATTGTGGA ATAAAGTCAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019674688.1
CDS	541..5145
Translation	MVRPVSQETGHLLNFSRAAMDGQAFQPDPWTPSFIDRSSACITPYMDAEREALQSTAYPEVQNFCQKHGLMFEVVDLRWGIRNTEATEHMTTELCLEELDRCRKTSTGPAFVALLGDQYGPCPVPTLIEEKEWEALRAQLTSKPRDLELVAQHFRRDENAVPPTYLLQALGTSEACGPEEATLTSVLRSGAQEARRLGLITQEQWHRYHWSVIEWEIDRGLLSLTDGDRGATIFLREIQDLNKHILEDCALKMVDRLPDGCLDTDAQNLLRGLKGRIADVQPGVLRAHHLPWSRDLVNPKNKAHARYLKELGEQFVARASHQVLEHLRELEAGRQELAWLYQEIRHHLGLSAEANRTFCGRQELLAQLGQRLQQSDSHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGASQMSSDARGLLQSICAQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPKCPPRIHLILSACSGRQGILDTVRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAMHQLCARLEQTHGQVLVAHVLGYIASSRHGLSEAELKDVLSLDDEVLQVVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLAEVVQERYLPGVERAKRHGVLADFFSGAWSQGTKKLVTLPLVGKPLNLDRMVAPQPLWFSDTVANLRKLKEMPHHLVHSGRLETLKQEVLGSMNWISCRGISGGIESLLDDFDLYAPYLDCPEVGLVREALQLCRPALELRGMERSILYTELLARLHFFTTSHPALVGQLCQQAQNWFRMCPHPVLVPLTGFLQPPGGPLRATLTGCHKGITAMAWSVEERLLVVGTQDGTVAVWDTEEHQVIHMHMGHTGEVRYVKVFAKGTLALSASKDHTLRLWNLLTGQEKFTIWDGGSKDPMQPETWSLHLDETKKVVYLASGSKVSAWHLETAELVFQILGDPSDCWVYAGVLASRATLLTVSQGGVAGLWSSATGKPQGRQRLSSIKEETPTCGVSLQKQGKMVTGFSKGSVSLISPEGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVETASFGPENNLIVTGSRDALIQVWSLSEQGTLLEVLEGVGAPVSLLVWGGALVASASQQSSSFKVWDLTSVRQPRASTPFLDRTGLTAVSHDGSYVYFPKIGDKNKVTIWDLAEGEEQDVLDASSEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSQGEERLAVAYDSIILVLDISPGDPCPVVDGPTYTFYTQLPETISSVAVLADYRVIYGMTDGELFLYECANSKVFPLEAHGSRVTCVQVSHKEQLAVSGSEEALLCLWDLQACKWRFETSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDAPWSSFLCARQDTAQFLNCGIKSI

Target ORF information:

RefSeq Version	XM_019819129.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus NACHT and WD repeat domain containing 1 (NWD1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019819129.1

ORF Insert Sequence:

ATGGTGCGGC CAGTGTCCCA GGAAACAGGC CACTTGCTTA ACTTCTCCAG GGCAGCAATG 
GACGGACAAG CATTCCAACC AGACCCATGG ACTCCCAGCT TTATTGACAG ATCATCAGCG 
TGTATAACAC CGTATATGGA TGCCGAGAGA GAAGCCCTAC AGAGCACCGC CTACCCCGAA 
GTGCAGAACT TCTGCCAAAA GCACGGTCTG ATGTTTGAGG TTGTTGATCT CAGGTGGGGT 
ATTCGGAACA CTGAGGCCAC TGAGCACATG ACCACAGAAC TCTGCTTGGA GGAACTCGAC 
CGGTGTCGGA AAACATCCAC AGGGCCGGCT TTTGTTGCCC TTCTCGGTGA CCAGTACGGC 
CCCTGTCCAG TCCCCACGCT GATCGAGGAG AAGGAGTGGG AGGCTCTGAG GGCCCAGCTG 
ACCTCCAAGC CACGCGACCT GGAGTTGGTA GCTCAACACT TCCGGAGGGA CGAGAACGCC 
GTGCCCCCCA CCTACCTGCT GCAGGCCCTG GGCACCTCGG AGGCCTGTGG GCCCGAAGAG 
GCCACGCTGA CCTCAGTGCT ACGCTCTGGA GCCCAGGAGG CCCGGAGGCT GGGCCTCATC 
ACCCAGGAGC AGTGGCATCG TTATCACTGG TCAGTCATCG AGTGGGAGAT AGACCGGGGC 
CTGTTGAGCT TGACGGATGG AGACCGGGGG GCAACCATCT TCTTGAGAGA GATCCAAGAC 
CTCAACAAAC ACATCCTGGA AGACTGCGCC CTCAAGATGG TGGACCGGCT CCCAGATGGC 
TGCCTGGACA CAGACGCCCA GAACCTTCTC CGTGGCCTCA AAGGACGCAT TGCTGACGTG 
CAACCGGGAG TTCTCAGGGC TCACCACCTG CCGTGGAGCC GAGACCTGGT GAACCCTAAA 
AACAAGGCTC ATGCTCGCTA CCTGAAGGAG CTGGGGGAAC AGTTTGTGGC CAGGGCTAGC 
CACCAGGTCC TGGAGCACCT CCGGGAGCTG GAGGCGGGCA GGCAGGAGTT GGCGTGGCTC 
TATCAGGAGA TCCGCCACCA CCTGGGGCTG AGTGCCGAGG CCAATCGGAC TTTCTGTGGG 
CGCCAGGAGC TCCTGGCCCA GCTGGGGCAG CGGCTCCAGC AGAGCGACAG CCACCCCCAC 
TCTCCCCTGG TGCTCTTTGG GCCCCCCGGC ATCGGAAAGA CAGCTCTGAT GTGCAAACTG 
GCGGAACAAA TGCCAGGCCT GCTTGGCCGC AAGACGGTGA CCGTCCTGCG GCTTCTGGGA 
GCGTCACAGA TGAGCTCCGA CGCCCGAGGC CTGCTCCAGA GCATCTGCGC CCAGGTGTGC 
CTGGCCTACG GGTTGCCCCT GCCCCCTGCC CAGGTCCTGG AGGCCCACGC CAGGGTGGTG 
CAGTTTTTCC ACACCCTCCT CCACACCGTC TCCTGCCGCA ACTTCGAGTC CCTTGTGATC 
CTGTTGGATT CCATGGACGA TGTGGATTCC ATCTGCCGCG CCCGGAGGGT CCCCTGGCTG 
CCTCCCAAAT GCCCCCCGAG GATCCACCTT ATCCTCTCAG CCTGCTCAGG GCGCCAGGGG 
ATTCTAGACA CCGTGCGACA GACGCTGACG GACCCGGAGG CCTACTGGGA GGTGAAACCC 
CTCTCCGGAA ACCAAGGGCA AGAGATGATC CAGCTCCTGC TGGCCGCCTC GAGGAGGACG 
CTGAGCCCAG GGCAGAGGGA ACTGCTCTGG GCCAGCCTCC CAGAGTGCGG GCACCCAGGG 
CGGCTGAGGC TGGCCTTCGA GGAGGCCCGG AAATGGGCCT CCTTCACCAT ACCTGCCCCC 
CTGGCCTCCA CTGCCAAGGA AGCCATGCAC CAACTGTGTG CCCGCCTGGA GCAGACACAC 
GGGCAGGTCC TGGTGGCCCA CGTGCTGGGC TACATCGCGT CTTCCCGGCA TGGGCTCTCA 
GAGGCAGAGC TGAAGGACGT CCTGTCCTTG GATGACGAGG TCCTGCAGGT GGTCTACCGG 
GACTGGACCC CGCCCAGCAA GGAGCTACTG CGTTTTCCAC CGCTGCTCTG GGTGAGGCTT 
CGCCGGGATC TGGGCCACTG CTTGGCCCGG CGGCCCGTGG ACGGCTTCAC GCTCCTGGCC 
ATTGCCCACA GGCAGCTGGC CGAGGTGGTC CAAGAACGCT ACCTGCCCGG GGTCGAGAGA 
GCCAAGAGGC ACGGGGTCTT GGCTGACTTC TTCTCAGGGG CCTGGAGCCA GGGCACCAAG 
AAGCTCGTCA CCCTGCCGCT CGTGGGGAAG CCCCTGAACC TGGACCGCAT GGTGGCCCCT 
CAGCCCCTGT GGTTCTCGGA CACGGTCGCA AATCTGCGAA AGCTGAAGGA GATGCCTCAT 
CACCTGGTCC ACTCAGGCCG CCTTGAAACG CTGAAGCAGG AGGTTCTGGG CAGCATGAAC 
TGGATTTCCT GCCGCGGCAT CTCTGGAGGC ATCGAGAGCC TGTTGGACGA CTTTGACCTG 
TATGCCCCTT ACCTGGACTG TCCGGAGGTC GGCCTGGTCC GGGAAGCCCT CCAGCTGTGC 
CGCCCGGCCC TGGAGCTCCG AGGCATGGAG AGGAGCATCC TATATACAGA ACTCCTGGCC 
AGACTCCATT TCTTCACGAC CTCACATCCG GCGCTGGTGG GACAGCTGTG CCAACAGGCC 
CAGAACTGGT TCCGGATGTG CCCACATCCT GTGCTGGTAC CCCTCACGGG ATTCCTCCAG 
CCCCCGGGAG GACCCCTCCG GGCAACCCTC ACCGGCTGTC ACAAGGGCAT CACGGCCATG 
GCGTGGAGCG TGGAAGAGAG GCTGCTGGTT GTCGGCACCC AGGATGGCAC CGTGGCTGTG 
TGGGACACGG AAGAACATCA GGTGATCCAT ATGCACATGG GACACACAGG AGAAGTGAGG 
TATGTGAAGG TGTTTGCCAA AGGGACACTT GCCCTCTCTG CCTCAAAGGA CCATACACTG 
CGCTTGTGGA ATCTACTGAC TGGCCAGGAG AAATTCACCA TTTGGGATGG AGGCTCAAAG 
GATCCCATGC AACCTGAGAC CTGGAGTCTT CACTTGGATG AAACAAAGAA GGTTGTGTAT 
TTGGCATCTG GCTCAAAGGT CAGTGCGTGG CATCTAGAAA CTGCAGAACT GGTTTTCCAG 
ATCCTGGGAG ATCCCTCTGA CTGCTGGGTG TATGCAGGAG TGCTGGCTTC CCGGGCCACG 
CTGCTGACCG TGTCCCAGGG TGGCGTGGCC GGCCTGTGGA GCTCAGCCAC AGGAAAGCCG 
CAGGGGAGGC AACGTTTATC CAGCATCAAG GAAGAAACGC CTACCTGTGG GGTCTCGCTC 
CAGAAGCAGG GGAAGATGGT CACCGGGTTC AGCAAGGGCT CCGTCTCCTT GATTTCCCCC 
GAAGGAGACA GCCTGCTGGA GAAGCTTCCG GAAGCTGTGA GGTTCCTGGT AGTCTCTGAA 
GATGAGTCCC TTCTCGCTGC AGGGTTTGGA AGATCCGTCC GGATATTCTT GGCAGACTCA 
CAGGGGTTTC ATCGATTCAT GGCCACCGAC CTTGAACACG AGGACACCGT GGAGACAGCC 
AGTTTTGGTC CTGAGAACAA TCTGATTGTC ACCGGGTCCC GGGACGCCCT CATTCAGGTG 
TGGAGTCTGT CGGAGCAGGG GACCCTGCTG GAAGTTCTGG AAGGCGTCGG GGCCCCCGTG 
AGCCTGTTAG TCTGGGGTGG GGCCTTGGTG GCATCTGCTT CCCAGCAGTC CTCGTCTTTC 
AAAGTCTGGG ATCTCACGTC TGTGCGGCAG CCGAGGGCCT CTACCCCGTT TCTGGATCGC 
ACTGGCCTCA CTGCGGTATC ACACGATGGA AGCTATGTTT ACTTCCCCAA GATTGGGGAC 
AAGAACAAAG TCACCATTTG GGACTTAGCA GAAGGTGAGG AGCAGGACGT CCTGGATGCC 
TCCAGTGAGG TCAGATGTCT GGAGGTGGCC CAGCAGGCCA ATCTCCTTTT CACTGGCCTC 
GTTTCGGGGG TCGTCCTGGT GTTCCCCCTG AATTCCAGAC AGGACGTGAT GTGCATCCCC 
CCTCCCGAGG CCCGGAAAGC CATCAACTGC ATGGCCCTCA GTCAAGGAGA GGAGCGCCTG 
GCCGTCGCCT ATGACAGCAT CATCCTGGTG CTGGACATCA GCCCCGGGGA TCCCTGTCCG 
GTTGTCGACG GGCCAACATA CACCTTCTAC ACCCAGCTGC CTGAGACCAT CTCCAGCGTG 
GCCGTCCTGG CTGACTACCG TGTGATCTAC GGCATGACCG ACGGTGAGCT CTTCCTTTAC 
GAGTGTGCGA ATTCCAAAGT GTTCCCTCTG GAGGCCCACG GGAGCCGGGT CACCTGTGTG 
CAGGTCAGCC ACAAGGAGCA GCTGGCGGTC AGCGGATCCG AGGAAGCCCT GCTGTGCCTC 
TGGGACCTGC AGGCCTGCAA GTGGAGGTTC GAGACGAGCT ACACGAATTC TTACTGTAGA 
GGAGTCCAGT GTGTCTGCTT CTCCAAGGAT GACAAGTACG TGTATGCGGG CTTGAAGGAC 
CGCTCCATCA TCGTCTGGAG TGTGCTGGAT GCTCCCTGGA GCTCCTTTCT GTGTGCTAGA 
CAGGATACCG CCCAATTCTT AAATTGTGGA ATAAAGTCAA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NWD1 ( XM_019819120.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019674679.2 Felis catus NACHT and WD repeat domain containing 1 (NWD1), mRNA.

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CloneID	OFb71700
Clone ID Related Accession (Same CDS sequence)	XM_019819120.2
Accession Version	XM_019819120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	LOW QUALITY PROTEIN: NACHT domain- and WD repeat-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018724.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019819120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGTGCGGC CAGTGTCCCG GGAAACAGGC CACTTGCTTA ACTTCTCCAG GGCAGCAATG 
GACGGACAAG CATTCCAACC AGACCCATGG ACTCCCAGCT TTATTGACAG ATCATCAGCG 
TGTATAACAC CGTATATGGA TGCCGAGAGA GAAGCCCTAC AGAGCACCGC CTACCCCGAA 
GTGCAGAACT TCTGCCAAAA GCACGGTCTG ATGTTTGAGG TTGTCGATCT CAGGTGGGGT 
ATTCGGAACA CTGAGGCCAC TGAGCACATG ACCACAGAAC TCTGCTTGGA GGAACTCGAC 
CGGTGTCGGA AAACATCCAC AGGGCCGGCT TTTGTTGCCC TTCTCGGTGA CCAGTACGGC 
CCCTGTCCAG TCCCCACGCT GATCGAGGAG AAGGAGTGGG AGGCTCTGAG GGCCCAGCTG 
ACCTCCAAGC CACGCGACCT GGAGTTGGTA GCTCAACACT TCCGGAGGGA CGAGAACGCC 
GTGCCCCCCA CCTACCTGCT GCAGGCCCTG GGCACCTCGG AGGCCTGTGG GCCCGAAGAG 
GCCACGCTGA CCTCAGTGCT ACGCTCTGGA GCCCAGGAGG CCCGGAGGCT GGGCCTCATC 
ACCCAGGAGC AGTGGCATCG TTATCACTGG TCAGTCATCG AGTGGGAGAT AGACCGGGGC 
CTGTTGAGCT TGACGGATGG AGACCGGGGG GCAACCATCT TCTTGAGAGA GATCCAAGAC 
CTCAACAAAC ACATCCTGGA AGACTGCGCC CTCAAGATGG TGGACCGGCT CCCAGATGGC 
TGCCTGGACA CAGACGCCCA GAACCTTCTC CGTGGCCTCA AAGGACGCAT TGCTGACGTG 
CAACCGGGAG TTCTCAGGGC TCACCACCTG CCGTGGAGCC GAGACCTGGT GAACCCTAAA 
AACAAGGCTC ATGCTCGCTA CCTGAAGGAG CTGGGGGAAC AGTTTGTGGC CAGGGCTAGC 
CACCAGGTCC TGGAGCACCT CCGGGAGCTG GAGGCGGGCA GGCAGGAGTT GGCGTGGCTC 
TATCAGGAGA TCCGCCACCA CCTGGGGCTG AGTGCCGAGG CCAATCGGAC TTTCTGTGGG 
CGCCAGGAGT TCCTGGCCCA GCTGGGGCAG CGGCTCCAGC AGAGCGACAG CCACCCCCAC 
TCTCCCCTGG TGCTCTTTGG GCCCCCCGGC ATCGGAAAGA CAGCTCTGAT GTGCAAACTG 
GCGGAACAAA TGCCAGGCCT GCTTGGCCGC AAGACGGTGA CCGTCCTGCG GCTTCTGGGA 
GCGTCACAGA TGAGCTCCGA CGCCCGAGGC CTGCTCCAGA GCATCTGCGC CCAGGTGTGC 
CTGGCCTACG GGTTGCCCCT GCCCCCTGCC CAGGTCCTGG AGGCCCACGC CAGGGTGGTG 
CAGTTTTTCC ACACCCTCCT CCACACCGTC TCCTGCCGCA ACTTCGAGTC CCTTGTGATC 
CTGTTGGATT CCATGGACGA TGTGGATTCC ATCTGCCGCG CCCGGAGGGT CCCCTGGCTG 
CCTCCCAAAT GCCCCCCGAG GATCCACCTT ATCCTCTCAG CCTGCTCAGG GCGCCAGGGG 
ATTCTAGACA CCGTGCGACA GACGCTGACG GACCCGGAGG CCTACTGGGA GGTGAAACCC 
CTCTCCGGAA ACCAAGGGCA AGAGATGATC CAGCTCCTGC TGGCCGCCTC GAGGAGGACG 
CTGAGCCCAG GGCAGAGGGA ACTGCTCTGG GCCAGCCTCC CAGAGTGCGG GCACCCAGGG 
CGGCTGAGGC TGGCCTTCGA GGAGGCCCGG AAATGGGCCT CCTTCACCAT ACCTGCCCCC 
CTGGCCTCCA CTGCCAAGGA AGCCATGCAC CAACTGTGTG CCCGCCTGGA GCAGACACAC 
GGGCAGGTCC TGGTGGCCCA CGTGCTGGGC TACATCGCGT CTTCCCGGCA TGGGCTCTCA 
GAGGCAGAGC TGAAGGACGT CCTGTCCTTG GATGACGAGG TCCTGCAGGT GGTCTACCGG 
GACTGGACCC CGCCCAGCAA GGAGCTACTG CGTTTTCCAC CGCTGCTCTG GGTGAGGCTT 
CGCCGGGATC TGGGCCACTG CTTGGCCCGG CGGCCCGTGG ACGGCTTCAC GCTCCTGGCC 
ATTGCCCACA GGCAGCTGGC CGAGGTGGTC CAAGAACGCT ACCTGCCCGG GGTCGAGAGA 
GCCAAGAGGC ACGGGGTCTT GGCTGACTTC TTCTCAGGGG CCTGGAGCCA GGGCACCAAG 
AAGCTCGTCA CCCTGCCGCT CGTGGGGAAG CCCCTGAACC TGGACCGCAT GGTGGCCCCT 
CAGCCCCTGT GGTTCTCGGA CACGGTCGCA AATCTGCGAA AGCTGAAGGA GATGCCTCAT 
CACCTGGTCC ACTCAGGCCG CCTTGAAACG CTGAAGCAGG AGGTTCTGGG CAACATGAAC 
TGGATTTCCT GCCGCGGCAT CTCTGGAGGC ATCGAGAGCC TGTTGGACGA CTTTGACCTG 
TATGCCCCTT ACCTGGACTG TCCGGAGGTC GGCCTGGTCC GGGAAGCCCT CCAGCTGTGC 
CGCCCGGCCC TGGAGCTCCG AGGCATGGAG AGGAGCATCC TATATACAGA ACTCCTGGCC 
AGACTCCATT TCTTCACGAC CTCACATCCG GCGCTGGTGG GACAGCTGTG CCAACAGGCC 
CAGAACTGGT TCCGGATGTG CCCACATCCT GTGCTGGTAC CCCTCACGGG ATTCCTCCAG 
CCCCCGGGAG GACCCCTCCG GGCAACCCTC ACCGGCTGTC ACAAGGGCAT CACGGCCATG 
GCGTGGAGCG TGGAAGAGAG GCTGCTGGTT GTCGGCACCC AGGATGGCAC CGTGGCTGTG 
TGGGACACGG AAGAACATCA GGTGATCCAT ATGCACATGG GACACACAGG AGAAGTGAGG 
TATGTGAAGG TGTTTGCCAA AGGGACACTT GCCCTCTCTG CCTCAAAGGA CCATACACTG 
CGCTTGTGGA ATCTACTGAC TGGCCAGGAG AAATTCACCA TTTGGGATGG AGGCTCAAAG 
GATCCCATGC AACCTGAGAC CTGGAGTCTT CACTTGGATG AAACAAAGAA GGTTGTGTAT 
TTGGCATCTG GCTCAAAGGT CAGTGCGTGG CATCTAGAAA CTGCAGAACT GGTTTTCCAG 
ATCCTGGGAG ATCCCTCTGA CTGCTGGGTG TATGCAGGAG TGCTGGCTTC CCGGGCCACG 
CTGCTGACCG TGTCCCAGGG TGGCGTGGCC GGCCTGTGGA GCTCAGCCAC AGGAAAGCCG 
CAGGGGAGGC AACGTTTATC CAGCATCAAG GAAGAAACGC CTACCTGTGG GGTCTCGCTC 
CAGAAGCAGG GGAAGATGGT CACCGGGTTC AGCAAGGGCT CCGTCTCCTT GATTTCCCCC 
GAAGGAGACA GCCTGCTGGA GAAGCTTCCG GAAGCTGTGA GGTTCCTGGT AGTCTCTGAA 
GATGAGTCCC TTCTCGCTGC AGGGTTTGGA AGATCCGTCC GGATATTCTT GGCAGACTCA 
CAGGGGTTTC ATCGATTCAT GGCCACCGAC CTTGAACACG AGGACACCGT GGAGACAGCC 
AGTTTTGGTC CTGAGAACAA TCTGATTGTC ACCGGGTCCC GGGACGCCCT CATTCAGGTG 
TGGAGTCTGT CGGAGCAGGG GACCCTGCTG GAAGTTCTGG AAGGCGTCGG GGCCCCCGTG 
AGCCTGTTAG TCTGGGGTGG GGCCTTGGTG GCATCTGCTT CCCAGCAGTC CTCGTCTTTC 
AAAGTCTGGG ATCTCACGTC TGTGCGGCAG CCGAGGGCCT CTACCCCGTT TCTGGATCGC 
ACTGGCCTCA CTGCGGTATC ACACGATGGA AGCTATGTTT ACTTCCCCAA GATTGGGGAC 
AAGAACAAAG TCACCATTTG GGACTTAGCA GAAGGTGAGG AGCAGGACGT CCTGGATGCC 
TCCAGTGAGG TCAGATGTCT GGAGGTGGCC CAGCAGGCCA ATCTCCTTTT CACTGGCCTC 
GTTTCGGGGG TCGTCCTGGT GTTCCCCCTG AATTCCAGAC AGGACGTGAT GTGCATCCCC 
CCTCCCGAGG CCCGGAAAGC CATCAACTGC ATGGCCCTCA GTCAAGGAGA GGAGCGCCTG 
GCCGTCGCCT ATGACAGCAT CATCCTGGTG CTGGACATCA GCCCCGGGGA TCCCTGTCCG 
GTTGTCGACG GGCCAACATA CACCTTCTAC ACCCAGCTGC CTGAGACCAT CTCCAGCGTG 
GCCGTCCTGG CTGACTACCG TGTGATCTAC GGCATGACCG ACGGTGAGCT CTTCCTTTAC 
GAGTGTGCGA ATTCCAAAGT GTTCCCTCTG GAGGCCCACG GGAGCCGGGT CACCTGTGTG 
CAGGTCAGCC ACAAGGAGCA GCTGGCGGTC AGCGGATCCG AGGAAGCCCT GCTGTGCCTC 
TGGGACCTGC AGGCCTGCAA GTGGAGGTTC GAGACGAGCT ACACGAATTC TTACTGTAGA 
GGAGTCCAGT GTGTCTGCTT CTCCAAGGAT GACAAGTACG TGTATGCGGG CTTGAAGGAC 
CGCTCCATCA TCGTCTGGAG TGTGCTGGAT GGCACCCTGC TGGCTGTCCA GTTTGTCCAC 
GCCGTGGTGA ACAGAATCAT CCCAACTTCC AGTGGCTTCA TCGCCCCCAC GAGACACGGC 
TATCTCATCC GGGAGCGTTT CCAGGACCCT TCGTCAAGAG CCTCGCAGCA GGACCCTCTC 
AAGAACTTCA AGAAGGCAGT GTGGAAGGTC AAATCCAGGC AGAGAGACGA AGCGGCGGCT 
GTCGCAGGAG GCCCCCAGGA CTCGAGATCA GCAAGTGTCC AGGGAAATGC GTGCAAATCG 
AACAAACGCT CACAAGTCTG CCTGCTCGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019674679.2
CDS	994..5826
Translation	MVRPVSRETGHLLNFSRAAMDGQAFQPDPWTPSFIDRSSACITPYMDAEREALQSTAYPEVQNFCQKHGLMFEVVDLRWGIRNTEATEHMTTELCLEELDRCRKTSTGPAFVALLGDQYGPCPVPTLIEEKEWEALRAQLTSKPRDLELVAQHFRRDENAVPPTYLLQALGTSEACGPEEATLTSVLRSGAQEARRLGLITQEQWHRYHWSVIEWEIDRGLLSLTDGDRGATIFLREIQDLNKHILEDCALKMVDRLPDGCLDTDAQNLLRGLKGRIADVQPGVLRAHHLPWSRDLVNPKNKAHARYLKELGEQFVARASHQVLEHLRELEAGRQELAWLYQEIRHHLGLSAEANRTFCGRQEFLAQLGQRLQQSDSHPHSPLVLFGPPGIGKTALMCKLAEQMPGLLGRKTVTVLRLLGASQMSSDARGLLQSICAQVCLAYGLPLPPAQVLEAHARVVQFFHTLLHTVSCRNFESLVILLDSMDDVDSICRARRVPWLPPKCPPRIHLILSACSGRQGILDTVRQTLTDPEAYWEVKPLSGNQGQEMIQLLLAASRRTLSPGQRELLWASLPECGHPGRLRLAFEEARKWASFTIPAPLASTAKEAMHQLCARLEQTHGQVLVAHVLGYIASSRHGLSEAELKDVLSLDDEVLQVVYRDWTPPSKELLRFPPLLWVRLRRDLGHCLARRPVDGFTLLAIAHRQLAEVVQERYLPGVERAKRHGVLADFFSGAWSQGTKKLVTLPLVGKPLNLDRMVAPQPLWFSDTVANLRKLKEMPHHLVHSGRLETLKQEVLGNMNWISCRGISGGIESLLDDFDLYAPYLDCPEVGLVREALQLCRPALELRGMERSILYTELLARLHFFTTSHPALVGQLCQQAQNWFRMCPHPVLVPLTGFLQPPGGPLRATLTGCHKGITAMAWSVEERLLVVGTQDGTVAVWDTEEHQVIHMHMGHTGEVRYVKVFAKGTLALSASKDHTLRLWNLLTGQEKFTIWDGGSKDPMQPETWSLHLDETKKVVYLASGSKVSAWHLETAELVFQILGDPSDCWVYAGVLASRATLLTVSQGGVAGLWSSATGKPQGRQRLSSIKEETPTCGVSLQKQGKMVTGFSKGSVSLISPEGDSLLEKLPEAVRFLVVSEDESLLAAGFGRSVRIFLADSQGFHRFMATDLEHEDTVETASFGPENNLIVTGSRDALIQVWSLSEQGTLLEVLEGVGAPVSLLVWGGALVASASQQSSSFKVWDLTSVRQPRASTPFLDRTGLTAVSHDGSYVYFPKIGDKNKVTIWDLAEGEEQDVLDASSEVRCLEVAQQANLLFTGLVSGVVLVFPLNSRQDVMCIPPPEARKAINCMALSQGEERLAVAYDSIILVLDISPGDPCPVVDGPTYTFYTQLPETISSVAVLADYRVIYGMTDGELFLYECANSKVFPLEAHGSRVTCVQVSHKEQLAVSGSEEALLCLWDLQACKWRFETSYTNSYCRGVQCVCFSKDDKYVYAGLKDRSIIVWSVLDGTLLAVQFVHAVVNRIIPTSSGFIAPTRHGYLIRERFQDPSSRASQQDPLKNFKKAVWKVKSRQRDEAAAVAGGPQDSRSASVQGNACKSNKRSQVCLLV

Target ORF information:

RefSeq Version	XM_019819120.2
Organism	Felis catus(domestic cat)
Definition	Felis catus NACHT and WD repeat domain containing 1 (NWD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019819120.2

ORF Insert Sequence:

ATGGTGCGGC CAGTGTCCCG GGAAACAGGC CACTTGCTTA ACTTCTCCAG GGCAGCAATG 
GACGGACAAG CATTCCAACC AGACCCATGG ACTCCCAGCT TTATTGACAG ATCATCAGCG 
TGTATAACAC CGTATATGGA TGCCGAGAGA GAAGCCCTAC AGAGCACCGC CTACCCCGAA 
GTGCAGAACT TCTGCCAAAA GCACGGTCTG ATGTTTGAGG TTGTCGATCT CAGGTGGGGT 
ATTCGGAACA CTGAGGCCAC TGAGCACATG ACCACAGAAC TCTGCTTGGA GGAACTCGAC 
CGGTGTCGGA AAACATCCAC AGGGCCGGCT TTTGTTGCCC TTCTCGGTGA CCAGTACGGC 
CCCTGTCCAG TCCCCACGCT GATCGAGGAG AAGGAGTGGG AGGCTCTGAG GGCCCAGCTG 
ACCTCCAAGC CACGCGACCT GGAGTTGGTA GCTCAACACT TCCGGAGGGA CGAGAACGCC 
GTGCCCCCCA CCTACCTGCT GCAGGCCCTG GGCACCTCGG AGGCCTGTGG GCCCGAAGAG 
GCCACGCTGA CCTCAGTGCT ACGCTCTGGA GCCCAGGAGG CCCGGAGGCT GGGCCTCATC 
ACCCAGGAGC AGTGGCATCG TTATCACTGG TCAGTCATCG AGTGGGAGAT AGACCGGGGC 
CTGTTGAGCT TGACGGATGG AGACCGGGGG GCAACCATCT TCTTGAGAGA GATCCAAGAC 
CTCAACAAAC ACATCCTGGA AGACTGCGCC CTCAAGATGG TGGACCGGCT CCCAGATGGC 
TGCCTGGACA CAGACGCCCA GAACCTTCTC CGTGGCCTCA AAGGACGCAT TGCTGACGTG 
CAACCGGGAG TTCTCAGGGC TCACCACCTG CCGTGGAGCC GAGACCTGGT GAACCCTAAA 
AACAAGGCTC ATGCTCGCTA CCTGAAGGAG CTGGGGGAAC AGTTTGTGGC CAGGGCTAGC 
CACCAGGTCC TGGAGCACCT CCGGGAGCTG GAGGCGGGCA GGCAGGAGTT GGCGTGGCTC 
TATCAGGAGA TCCGCCACCA CCTGGGGCTG AGTGCCGAGG CCAATCGGAC TTTCTGTGGG 
CGCCAGGAGT TCCTGGCCCA GCTGGGGCAG CGGCTCCAGC AGAGCGACAG CCACCCCCAC 
TCTCCCCTGG TGCTCTTTGG GCCCCCCGGC ATCGGAAAGA CAGCTCTGAT GTGCAAACTG 
GCGGAACAAA TGCCAGGCCT GCTTGGCCGC AAGACGGTGA CCGTCCTGCG GCTTCTGGGA 
GCGTCACAGA TGAGCTCCGA CGCCCGAGGC CTGCTCCAGA GCATCTGCGC CCAGGTGTGC 
CTGGCCTACG GGTTGCCCCT GCCCCCTGCC CAGGTCCTGG AGGCCCACGC CAGGGTGGTG 
CAGTTTTTCC ACACCCTCCT CCACACCGTC TCCTGCCGCA ACTTCGAGTC CCTTGTGATC 
CTGTTGGATT CCATGGACGA TGTGGATTCC ATCTGCCGCG CCCGGAGGGT CCCCTGGCTG 
CCTCCCAAAT GCCCCCCGAG GATCCACCTT ATCCTCTCAG CCTGCTCAGG GCGCCAGGGG 
ATTCTAGACA CCGTGCGACA GACGCTGACG GACCCGGAGG CCTACTGGGA GGTGAAACCC 
CTCTCCGGAA ACCAAGGGCA AGAGATGATC CAGCTCCTGC TGGCCGCCTC GAGGAGGACG 
CTGAGCCCAG GGCAGAGGGA ACTGCTCTGG GCCAGCCTCC CAGAGTGCGG GCACCCAGGG 
CGGCTGAGGC TGGCCTTCGA GGAGGCCCGG AAATGGGCCT CCTTCACCAT ACCTGCCCCC 
CTGGCCTCCA CTGCCAAGGA AGCCATGCAC CAACTGTGTG CCCGCCTGGA GCAGACACAC 
GGGCAGGTCC TGGTGGCCCA CGTGCTGGGC TACATCGCGT CTTCCCGGCA TGGGCTCTCA 
GAGGCAGAGC TGAAGGACGT CCTGTCCTTG GATGACGAGG TCCTGCAGGT GGTCTACCGG 
GACTGGACCC CGCCCAGCAA GGAGCTACTG CGTTTTCCAC CGCTGCTCTG GGTGAGGCTT 
CGCCGGGATC TGGGCCACTG CTTGGCCCGG CGGCCCGTGG ACGGCTTCAC GCTCCTGGCC 
ATTGCCCACA GGCAGCTGGC CGAGGTGGTC CAAGAACGCT ACCTGCCCGG GGTCGAGAGA 
GCCAAGAGGC ACGGGGTCTT GGCTGACTTC TTCTCAGGGG CCTGGAGCCA GGGCACCAAG 
AAGCTCGTCA CCCTGCCGCT CGTGGGGAAG CCCCTGAACC TGGACCGCAT GGTGGCCCCT 
CAGCCCCTGT GGTTCTCGGA CACGGTCGCA AATCTGCGAA AGCTGAAGGA GATGCCTCAT 
CACCTGGTCC ACTCAGGCCG CCTTGAAACG CTGAAGCAGG AGGTTCTGGG CAACATGAAC 
TGGATTTCCT GCCGCGGCAT CTCTGGAGGC ATCGAGAGCC TGTTGGACGA CTTTGACCTG 
TATGCCCCTT ACCTGGACTG TCCGGAGGTC GGCCTGGTCC GGGAAGCCCT CCAGCTGTGC 
CGCCCGGCCC TGGAGCTCCG AGGCATGGAG AGGAGCATCC TATATACAGA ACTCCTGGCC 
AGACTCCATT TCTTCACGAC CTCACATCCG GCGCTGGTGG GACAGCTGTG CCAACAGGCC 
CAGAACTGGT TCCGGATGTG CCCACATCCT GTGCTGGTAC CCCTCACGGG ATTCCTCCAG 
CCCCCGGGAG GACCCCTCCG GGCAACCCTC ACCGGCTGTC ACAAGGGCAT CACGGCCATG 
GCGTGGAGCG TGGAAGAGAG GCTGCTGGTT GTCGGCACCC AGGATGGCAC CGTGGCTGTG 
TGGGACACGG AAGAACATCA GGTGATCCAT ATGCACATGG GACACACAGG AGAAGTGAGG 
TATGTGAAGG TGTTTGCCAA AGGGACACTT GCCCTCTCTG CCTCAAAGGA CCATACACTG 
CGCTTGTGGA ATCTACTGAC TGGCCAGGAG AAATTCACCA TTTGGGATGG AGGCTCAAAG 
GATCCCATGC AACCTGAGAC CTGGAGTCTT CACTTGGATG AAACAAAGAA GGTTGTGTAT 
TTGGCATCTG GCTCAAAGGT CAGTGCGTGG CATCTAGAAA CTGCAGAACT GGTTTTCCAG 
ATCCTGGGAG ATCCCTCTGA CTGCTGGGTG TATGCAGGAG TGCTGGCTTC CCGGGCCACG 
CTGCTGACCG TGTCCCAGGG TGGCGTGGCC GGCCTGTGGA GCTCAGCCAC AGGAAAGCCG 
CAGGGGAGGC AACGTTTATC CAGCATCAAG GAAGAAACGC CTACCTGTGG GGTCTCGCTC 
CAGAAGCAGG GGAAGATGGT CACCGGGTTC AGCAAGGGCT CCGTCTCCTT GATTTCCCCC 
GAAGGAGACA GCCTGCTGGA GAAGCTTCCG GAAGCTGTGA GGTTCCTGGT AGTCTCTGAA 
GATGAGTCCC TTCTCGCTGC AGGGTTTGGA AGATCCGTCC GGATATTCTT GGCAGACTCA 
CAGGGGTTTC ATCGATTCAT GGCCACCGAC CTTGAACACG AGGACACCGT GGAGACAGCC 
AGTTTTGGTC CTGAGAACAA TCTGATTGTC ACCGGGTCCC GGGACGCCCT CATTCAGGTG 
TGGAGTCTGT CGGAGCAGGG GACCCTGCTG GAAGTTCTGG AAGGCGTCGG GGCCCCCGTG 
AGCCTGTTAG TCTGGGGTGG GGCCTTGGTG GCATCTGCTT CCCAGCAGTC CTCGTCTTTC 
AAAGTCTGGG ATCTCACGTC TGTGCGGCAG CCGAGGGCCT CTACCCCGTT TCTGGATCGC 
ACTGGCCTCA CTGCGGTATC ACACGATGGA AGCTATGTTT ACTTCCCCAA GATTGGGGAC 
AAGAACAAAG TCACCATTTG GGACTTAGCA GAAGGTGAGG AGCAGGACGT CCTGGATGCC 
TCCAGTGAGG TCAGATGTCT GGAGGTGGCC CAGCAGGCCA ATCTCCTTTT CACTGGCCTC 
GTTTCGGGGG TCGTCCTGGT GTTCCCCCTG AATTCCAGAC AGGACGTGAT GTGCATCCCC 
CCTCCCGAGG CCCGGAAAGC CATCAACTGC ATGGCCCTCA GTCAAGGAGA GGAGCGCCTG 
GCCGTCGCCT ATGACAGCAT CATCCTGGTG CTGGACATCA GCCCCGGGGA TCCCTGTCCG 
GTTGTCGACG GGCCAACATA CACCTTCTAC ACCCAGCTGC CTGAGACCAT CTCCAGCGTG 
GCCGTCCTGG CTGACTACCG TGTGATCTAC GGCATGACCG ACGGTGAGCT CTTCCTTTAC 
GAGTGTGCGA ATTCCAAAGT GTTCCCTCTG GAGGCCCACG GGAGCCGGGT CACCTGTGTG 
CAGGTCAGCC ACAAGGAGCA GCTGGCGGTC AGCGGATCCG AGGAAGCCCT GCTGTGCCTC 
TGGGACCTGC AGGCCTGCAA GTGGAGGTTC GAGACGAGCT ACACGAATTC TTACTGTAGA 
GGAGTCCAGT GTGTCTGCTT CTCCAAGGAT GACAAGTACG TGTATGCGGG CTTGAAGGAC 
CGCTCCATCA TCGTCTGGAG TGTGCTGGAT GGCACCCTGC TGGCTGTCCA GTTTGTCCAC 
GCCGTGGTGA ACAGAATCAT CCCAACTTCC AGTGGCTTCA TCGCCCCCAC GAGACACGGC 
TATCTCATCC GGGAGCGTTT CCAGGACCCT TCGTCAAGAG CCTCGCAGCA GGACCCTCTC 
AAGAACTTCA AGAAGGCAGT GTGGAAGGTC AAATCCAGGC AGAGAGACGA AGCGGCGGCT 
GTCGCAGGAG GCCCCCAGGA CTCGAGATCA GCAAGTGTCC AGGGAAATGC GTGCAAATCG 
AACAAACGCT CACAAGTCTG CCTGCTCGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NWD1 cDNA ORF clone, Felis catus(domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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