LOC659344 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC659344
Entrez Gene ID	659344
Full Name	ATP-binding cassette sub-family A member 3
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015984873.1	XP_015840359.1	ATP-binding cassette sub-family A member 3 isoform X3
XM_008200926.2	XP_008199148.1	ATP-binding cassette sub-family A member 3 isoform X1
XM_008200928.2	XP_008199150.1	ATP-binding cassette sub-family A member 3 isoform X2
XM_008200931.2	XP_008199153.1	ATP-binding cassette sub-family A member 3 isoform X4
XM_008200929.2	XP_008199151.1	ATP-binding cassette sub-family A member 3 isoform X3
XM_015984872.1	XP_015840358.1	ATP-binding cassette sub-family A member 3 isoform X3
XM_008200930.2	XP_008199152.1	ATP-binding cassette sub-family A member 3 isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC659344 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC659344 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTg14530	XM_008200926.2 Latest version!	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTg14532	XM_008200928.2 Latest version!	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTg14533	XM_015984872.1 Latest version!	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTg14533	XM_008200930.2 Latest version!	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTg14533	XM_008200929.2 Latest version!	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTg14533	XM_015984873.1 Latest version!	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTg14534	XM_008200931.2 Latest version!	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC659344 ( XM_008200926.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008199148.1 Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTg14530
Clone ID Related Accession (Same CDS sequence)	XM_008200926.2
Accession Version	XM_008200926.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	ATP-binding cassette sub-family A member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015451937.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008200926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTA CATCAAGAGA AAATTGTTCA TATTCATTAA TTGGACAGGA AAGAATACAA 
AGTGCTGAAA ATGATGAATT TCGTTCTGTT GGAACAATGG GGAAGAAACT GGACAAGTTC 
CTTCTACTGA TGTGGAAGAA TTGGTTGCTG CAATACCGGA AGCCTCTCCA AACCCTTGTA 
GAAATTCTAG CTCCAGTCCT GTTCTCCATC CTCCTAGTCG TTATTCGAAG TCTGGTTGAT 
CCTGAAACGA ATGATCCAAA GATTTACACG CCCTTCTGTC TACTCCCAAT TTTATGCTCG 
GATGAAAGTA TTAGCACAAA TCCGAAATTT CTAACGAACC TCGGAAATAC GACGTTCAGA 
AACTTTACAC TAGTGTATTC ACCTTACTCA AACAAAGCCG TAAGACGGTC AATGGGAATT 
CTTGAAACTG TTTTGGGTGG CCACGTGGTT GGTTACAACA ACTCTGACCA ACTAGAAGCC 
CATTTCCTCG ATTCGAATTC CTCTCTCACG TTTGCTGCCA TCCAACTTGA TGACTCTTTA 
GCGCACCAAG ACACTCTACC AGACGACTTG GAAGTATCGC TGAGATTTCC AAGCGAACTT 
CGGATTGGGT TCGATTTGTT TGGGCAAAAC TGGCACACAA ATCTTTTGTA TCCTGTGTAT 
CAAGTACCAG GCCCCCGAGC GCCAGAGAAC AACACAGGGG CTCTCCCAGA AAAGTTACGC 
GATTTAGATA GAAGAAAAAG AACGTGGAGA ACTAATCTTT TATTTCCCAT TTATCAATCA 
CCTGGTCCTA GATCCCCAAG ATCAGACATT GGTGGTAAAC CAGGTTACTT CAAAGAAGGA 
TTCTTGACCT TACAACTAGT CATGACGTAC ATTTTTGCAA TTGGCGGACA CCTTGAAGCG 
CAAAATCTAA CACAGTTTCA AAATATTATT ACTACACTTC TGCCACCGCT AATTAAAATG 
CAACGTTTTC CGTATGGAAG TTGGATAGAG GATCCTTTGC TACAAGCTTT AGAAAGTTTC 
GTTGGAGTTA TTATTATGTT GAGTTTCGTT TACACTTGCA TAAACACGGT TAAAGTTATA 
ACAACCGAAA AAGAGAAACA GCTTAAGGAG GCTATGAAAA TTATGGGTTT GCCTAACTGG 
CTCCATTGGA CTGCGTGGTT TATCAAAACA TTTGTCTTTC TTTTAGTTTC AGTAGTTTTA 
ATTATTTTAC TTTTGAAAAT CTCGTGGTAT TCACACACCG AATATAGTGT TTTCACGTAC 
GCAGATCCCA CAGTAATGTT GGTGTTCTTA GTACTGTACG TTTGTGCAAC TATAACTTTT 
TGTTTTGCGA TTAGCGTATT TTTTTCTAAA GCAAATACAG CTGCAACAGT AGCTGGTCTT 
GTATGGTTCC TCTCTTATGC TCCATATTTA TTTTTACAAA AGCAGTATGA CCAACTCAGT 
CTCTCTACTA AACTCATAGC AAGTCTTGGG TCAAACACTG CAATGGCTTA CGGGTTCCAG 
TTAATGTTAA TGTATGAGGG TACCAGTGAA GGAATACAAT GGTCCAATAT AGGAAGACCC 
AATACTCCCG ATGACAGTCT AACGCTCGGT TACATAATGA TGATGCTGGT AATAGACACT 
TTTATCTATC TTTTTGTGGC TCTTTACGTC GAAGCCGTTT TTCCCGGGGA GTACGGAGTG 
CCACTTCCTT GGTACTTCCC GTTTACTTCC GCCTATTGGT GTGGTCATCC TAGCTATGTA 
GGAGTTGAAG ATTTCACTAG TGTCCCATCA CAAGAAGGTG AATTTTTTGA AAAAGAACCT 
AACAATCTTA CACCCGGTAT ACAAATTCGA AATTTGCGGA AAGTTTTTGA CCAAAAAGCA 
GCAGTTCGAA ATCTCTCGCT AAACATGTAC AACGATCAAA TTACGGTCCT TCTTGGACAC 
AATGGTGCCG GAAAAACTAC CACCATGTCC ATGCTGACTG GAATGTTTCC TCCCACAAGT 
GGTACTGCAG TCGTTTGTGG ACACGACATT AAAACGGATA TGGACGGAGT AAGAGAAAGT 
CTGGGTCTAT GTCCTCAACA TAACATCATC TTCGACGAAC TCACCGTCCG AGAGCATCTC 
TACTTTTTCA GTAAACTTAA AGGACTGAAA CGTGAAGCAA TCAATGAAGA AATTGATAAA 
TACGTCGAAC TGTTGGAACT GCAACCTAAA GCGAATGCAA AATCGGCCAC TTTGTCAGGA 
GGAATGAAGA GAAAACTTTG TGTGGGTATG GCATTGTGTG GCAACTCTAA AGTTGTCATG 
CTTGATGAAC CCACTGCCGG AATGGATCCA TCAGCAAGAC GAGCTTTGTG GGATTTGCTT 
CAAAATCAAA AACACGGAAG GACGATGCTT TTAACAACAC ATTTTATGGA CGAAGCTGAT 
TTATTAGGTG ATAGAATCGC TATTATGGCC GGAGGGGAGT TACAGTGTTG CGGCTCAAGT 
TTCTTCCTAA AGAAGAAATA CGGGACGGGA TACCATCTCA TAATGGATAA GGCTTCGAAT 
TGTCAAGAGG AACAAGTAAC ACAACTTTTG AAGCGACATA TTCCTAATAT CCAGGTACAC 
GGTAATGTTG GCTCTGAACT CACGTATCTA CTATCCGAAG ATCACTCTTC AGTTTTTGAA 
GAAATGCTGC GAGACTTAGA AGAAAATAGT GATAGTTTGG GTATTCGTAG TTACGGAATT 
TCACTAACAA CTTTAGAAGA AGTTTTCATG AAAGTCGGTG CTGACCACGG CCAGGAAGAA 
AGCTATAACG GTTTTGTGCA AGAAACAGAT TGTAATAAAA CAATGCTGGG TAAAATAAAA 
TCGAAATTGA AAAGGAATGA AAACTACGAA AATAGCATTA ACGAAACAAC TTTAAACCTT 
GAAACTTCTG CTCTTCTAAC CGGTTTCATG TTAATCAAAA ATCAGTTTTT GGCAATGCTT 
ATGAAAAAAA TTTTATCAAT CGTACGAACC TGGATATTGC AGTTTATTCA AATTTTGATG 
CCAGTTGCGT TTCTTATCAT AGCCATTGTC GTTAGCAGAA ACACTAACAA ATCAGCAGAT 
TTACCCAAAC TGCCCATAAC ATTAGATTCG TATCGCAATC CAGTAACGCT AATTGAAGAT 
AATGCAAATA ACTCATATTC GGCCTTGTAT ATGCAAAGCC TTAAAGGTTA TGAAATTCAA 
AGAGTGGCAA GCATCCACGA TGTAATGCTT AATTTGACCG TAACTGTTCC ATCCACTGTC 
AGACTAAGAT ACATTGTTGG AGCTAGTTTC AACAAAACAT TCGGCGGTGT TTTTACAACT 
ATCACAGCCT GGTTTAATAA TAACCCTTAC CACTCCCCGC CTCTAGCATT AGGTCTTGCC 
TTAAACGTGG CTTTCAAGCA TTTAGCTGGC ACAAATCGTT CAATAAGATG GGCAAATTAT 
CCGTTACCGT TTACAGCCGA TACAAAAGTG CAGCAACTGT TGGAAGGCAA CAGTATGGGT 
TTCCAGCTCG CTTTCAACAT CGGATTTAGT ATGGCTTTTG TATCCTCATT CTACGTCCTA 
TTTTATGTGC GGGAACGAGT TTGCAAGTCA AAACATTTAC AATTCGTGTC CGGAGTGAAA 
GTTTACATAT TCTGGATAAT GTCATTCGTC TGTGATATAA TCACGTTCAT TGTGACCATC 
ATCGCGATCA TCATTACTTT GGCGTGTTTC CAAGAAGATG GCTTCCGGTC GGCTGAGGAC 
TTAGGTCGCA TGTTCCTTCT TTTGTTCTAT TTTGGCGTTG CAATGTTGCC TATGATGTAC 
TTTGCGTCAT ACTTGTTCGA AGTACCTTCG ACAGGTTATA CTAGAATGAC CCTCTTTAGT 
GTTTTCACTG GAGTGGCTGC CTTTTTGGTT GTTCAAGTTT TGGCAACACC TGGATTGGAT 
TTGGAATATG TCGCGAATAC TCTACATTGG ATTTTCCTGG TAGTACCCCA TTATTCGTTA 
GCTACAGGTA TTAGAGACGT TTATACAACA TTTGCCACTA ATAAAATGTG CAAATACGCC 
GTCCAAACGT GCGTCGAGAA TAGGCCAGAT ATGACTGTGG ACAAGTGTTG GGATTTGACT 
TGTCATAATG CAAGTGCTGT GCTACAGGAT TACTGTTGTG TACGCGAGCC GCACTATTAC 
AAATGGGCCA GTCCTGGAAT AGGCCGAAAT TTGCTATACT CATTTTCCGT TGGAATAGTC 
CTATTTATTC TACTCAACAT AAAAGAATAT AAAATATTTT CAAAAACGAT AGACCATATT 
GTTGAGAGCT ATAGAAACAA ATTGCCCCAG CCAGTGGAAA ATGAAGACAG CGATGTCGCG 
GAAGAAAAAC ACAAAATCCA CAACACTTCT GAAACAGAGT TACAAAAAGA CTACATTTTG 
GTGCTTAAAG ATTTGACAAA GTATTACAAC AACTTTTTGG CAGTTAACGG ATTGTGTCTG 
GGTGTTAAGG AACGTGAATG CTTCGGATTA TTGGGTATAA ACGGTGCTGG GAAAACCACA 
ACTTTTAAAA TGATGACCGG TGATGTAAAA ATCACGCACG GAGAAGCTTG GGTGAAAAAA 
TTCAGTATCA AATCGCAACT AAAACAAGTG CAAAAGTTGA TTGGATATTG TCCGCAATTT 
GACGCTCTTC TTGATGACCT GACTGCCAAA GAATCATTGG TAATGTTTGC ACTTTTGAGA 
GGAGTTCCAA TGGACGAGTG CCGATACCTG GTCCAGAAAC TATCGACAGA TTTTGATTTC 
CAAAGACATT TGAATAAAAA GGTTAAGGAA CTTAGTGGTG GTAACAAGAG GAAGTTAAGT 
ACTGCCATTT CGCTGATTGG CGATCCTCCG GTGATATATT TAGACGAACC TACGACAGGA 
ATGGACCCTG CGACGAAACG CTATTTGTGG GATGCCATCT GCAAAGTTAG AGATAACGGA 
AAATGTGTAG TCTTGACCTC TCATAGTATG GAAGAATGTG AAGCTTTGTG CACAAGACTA 
GCCATAATGG TTAATGGCAA TTTCAAATGT TTGGGTTCCA CTCAACATTT GAAAAATAAA 
TTTGCCGAAG GGTATACGTT GACAATTAAA ATTAAGAAAA GCCCTGGCGG GGAAGATGCC 
GATATTAAAC CAATTGAAAA TTTCATCTCG GACCATTTTC CCAAAGCAAT ACAAAGAGAA 
AAACATCAAG AGCTTTTAAC ATATTATATT GTAGATAAAA GCATCCCCTG GTCCAGAATG 
TTCGGGATTT TGGAAAAAGG AAAAAAGCAG CTAAATATTG AAGATTATTC TTTGGGTCAA 
TCAAGTTTGG AACAGGTATT TTTATCTTTC ACAAGGTTGC AACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008199148.1
CDS	111..5438
Translation	MASTSRENCSYSLIGQERIQSAENDEFRSVGTMGKKLDKFLLLMWKNWLLQYRKPLQTLVEILAPVLFSILLVVIRSLVDPETNDPKIYTPFCLLPILCSDESISTNPKFLTNLGNTTFRNFTLVYSPYSNKAVRRSMGILETVLGGHVVGYNNSDQLEAHFLDSNSSLTFAAIQLDDSLAHQDTLPDDLEVSLRFPSELRIGFDLFGQNWHTNLLYPVYQVPGPRAPENNTGALPEKLRDLDRRKRTWRTNLLFPIYQSPGPRSPRSDIGGKPGYFKEGFLTLQLVMTYIFAIGGHLEAQNLTQFQNIITTLLPPLIKMQRFPYGSWIEDPLLQALESFVGVIIMLSFVYTCINTVKVITTEKEKQLKEAMKIMGLPNWLHWTAWFIKTFVFLLVSVVLIILLLKISWYSHTEYSVFTYADPTVMLVFLVLYVCATITFCFAISVFFSKANTAATVAGLVWFLSYAPYLFLQKQYDQLSLSTKLIASLGSNTAMAYGFQLMLMYEGTSEGIQWSNIGRPNTPDDSLTLGYIMMMLVIDTFIYLFVALYVEAVFPGEYGVPLPWYFPFTSAYWCGHPSYVGVEDFTSVPSQEGEFFEKEPNNLTPGIQIRNLRKVFDQKAAVRNLSLNMYNDQITVLLGHNGAGKTTTMSMLTGMFPPTSGTAVVCGHDIKTDMDGVRESLGLCPQHNIIFDELTVREHLYFFSKLKGLKREAINEEIDKYVELLELQPKANAKSATLSGGMKRKLCVGMALCGNSKVVMLDEPTAGMDPSARRALWDLLQNQKHGRTMLLTTHFMDEADLLGDRIAIMAGGELQCCGSSFFLKKKYGTGYHLIMDKASNCQEEQVTQLLKRHIPNIQVHGNVGSELTYLLSEDHSSVFEEMLRDLEENSDSLGIRSYGISLTTLEEVFMKVGADHGQEESYNGFVQETDCNKTMLGKIKSKLKRNENYENSINETTLNLETSALLTGFMLIKNQFLAMLMKKILSIVRTWILQFIQILMPVAFLIIAIVVSRNTNKSADLPKLPITLDSYRNPVTLIEDNANNSYSALYMQSLKGYEIQRVASIHDVMLNLTVTVPSTVRLRYIVGASFNKTFGGVFTTITAWFNNNPYHSPPLALGLALNVAFKHLAGTNRSIRWANYPLPFTADTKVQQLLEGNSMGFQLAFNIGFSMAFVSSFYVLFYVRERVCKSKHLQFVSGVKVYIFWIMSFVCDIITFIVTIIAIIITLACFQEDGFRSAEDLGRMFLLLFYFGVAMLPMMYFASYLFEVPSTGYTRMTLFSVFTGVAAFLVVQVLATPGLDLEYVANTLHWIFLVVPHYSLATGIRDVYTTFATNKMCKYAVQTCVENRPDMTVDKCWDLTCHNASAVLQDYCCVREPHYYKWASPGIGRNLLYSFSVGIVLFILLNIKEYKIFSKTIDHIVESYRNKLPQPVENEDSDVAEEKHKIHNTSETELQKDYILVLKDLTKYYNNFLAVNGLCLGVKERECFGLLGINGAGKTTTFKMMTGDVKITHGEAWVKKFSIKSQLKQVQKLIGYCPQFDALLDDLTAKESLVMFALLRGVPMDECRYLVQKLSTDFDFQRHLNKKVKELSGGNKRKLSTAISLIGDPPVIYLDEPTTGMDPATKRYLWDAICKVRDNGKCVVLTSHSMEECEALCTRLAIMVNGNFKCLGSTQHLKNKFAEGYTLTIKIKKSPGGEDADIKPIENFISDHFPKAIQREKHQELLTYYIVDKSIPWSRMFGILEKGKKQLNIEDYSLGQSSLEQVFLSFTRLQR

Target ORF information:

RefSeq Version	XM_008200926.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008200926.2

ORF Insert Sequence:

ATGGCATCTA CATCAAGAGA AAATTGTTCA TATTCATTAA TTGGACAGGA AAGAATACAA 
AGTGCTGAAA ATGATGAATT TCGTTCTGTT GGAACAATGG GGAAGAAACT GGACAAGTTC 
CTTCTACTGA TGTGGAAGAA TTGGTTGCTG CAATACCGGA AGCCTCTCCA AACCCTTGTA 
GAAATTCTAG CTCCAGTCCT GTTCTCCATC CTCCTAGTCG TTATTCGAAG TCTGGTTGAT 
CCTGAAACGA ATGATCCAAA GATTTACACG CCCTTCTGTC TACTCCCAAT TTTATGCTCG 
GATGAAAGTA TTAGCACAAA TCCGAAATTT CTAACGAACC TCGGAAATAC GACGTTCAGA 
AACTTTACAC TAGTGTATTC ACCTTACTCA AACAAAGCCG TAAGACGGTC AATGGGAATT 
CTTGAAACTG TTTTGGGTGG CCACGTGGTT GGTTACAACA ACTCTGACCA ACTAGAAGCC 
CATTTCCTCG ATTCGAATTC CTCTCTCACG TTTGCTGCCA TCCAACTTGA TGACTCTTTA 
GCGCACCAAG ACACTCTACC AGACGACTTG GAAGTATCGC TGAGATTTCC AAGCGAACTT 
CGGATTGGGT TCGATTTGTT TGGGCAAAAC TGGCACACAA ATCTTTTGTA TCCTGTGTAT 
CAAGTACCAG GCCCCCGAGC GCCAGAGAAC AACACAGGGG CTCTCCCAGA AAAGTTACGC 
GATTTAGATA GAAGAAAAAG AACGTGGAGA ACTAATCTTT TATTTCCCAT TTATCAATCA 
CCTGGTCCTA GATCCCCAAG ATCAGACATT GGTGGTAAAC CAGGTTACTT CAAAGAAGGA 
TTCTTGACCT TACAACTAGT CATGACGTAC ATTTTTGCAA TTGGCGGACA CCTTGAAGCG 
CAAAATCTAA CACAGTTTCA AAATATTATT ACTACACTTC TGCCACCGCT AATTAAAATG 
CAACGTTTTC CGTATGGAAG TTGGATAGAG GATCCTTTGC TACAAGCTTT AGAAAGTTTC 
GTTGGAGTTA TTATTATGTT GAGTTTCGTT TACACTTGCA TAAACACGGT TAAAGTTATA 
ACAACCGAAA AAGAGAAACA GCTTAAGGAG GCTATGAAAA TTATGGGTTT GCCTAACTGG 
CTCCATTGGA CTGCGTGGTT TATCAAAACA TTTGTCTTTC TTTTAGTTTC AGTAGTTTTA 
ATTATTTTAC TTTTGAAAAT CTCGTGGTAT TCACACACCG AATATAGTGT TTTCACGTAC 
GCAGATCCCA CAGTAATGTT GGTGTTCTTA GTACTGTACG TTTGTGCAAC TATAACTTTT 
TGTTTTGCGA TTAGCGTATT TTTTTCTAAA GCAAATACAG CTGCAACAGT AGCTGGTCTT 
GTATGGTTCC TCTCTTATGC TCCATATTTA TTTTTACAAA AGCAGTATGA CCAACTCAGT 
CTCTCTACTA AACTCATAGC AAGTCTTGGG TCAAACACTG CAATGGCTTA CGGGTTCCAG 
TTAATGTTAA TGTATGAGGG TACCAGTGAA GGAATACAAT GGTCCAATAT AGGAAGACCC 
AATACTCCCG ATGACAGTCT AACGCTCGGT TACATAATGA TGATGCTGGT AATAGACACT 
TTTATCTATC TTTTTGTGGC TCTTTACGTC GAAGCCGTTT TTCCCGGGGA GTACGGAGTG 
CCACTTCCTT GGTACTTCCC GTTTACTTCC GCCTATTGGT GTGGTCATCC TAGCTATGTA 
GGAGTTGAAG ATTTCACTAG TGTCCCATCA CAAGAAGGTG AATTTTTTGA AAAAGAACCT 
AACAATCTTA CACCCGGTAT ACAAATTCGA AATTTGCGGA AAGTTTTTGA CCAAAAAGCA 
GCAGTTCGAA ATCTCTCGCT AAACATGTAC AACGATCAAA TTACGGTCCT TCTTGGACAC 
AATGGTGCCG GAAAAACTAC CACCATGTCC ATGCTGACTG GAATGTTTCC TCCCACAAGT 
GGTACTGCAG TCGTTTGTGG ACACGACATT AAAACGGATA TGGACGGAGT AAGAGAAAGT 
CTGGGTCTAT GTCCTCAACA TAACATCATC TTCGACGAAC TCACCGTCCG AGAGCATCTC 
TACTTTTTCA GTAAACTTAA AGGACTGAAA CGTGAAGCAA TCAATGAAGA AATTGATAAA 
TACGTCGAAC TGTTGGAACT GCAACCTAAA GCGAATGCAA AATCGGCCAC TTTGTCAGGA 
GGAATGAAGA GAAAACTTTG TGTGGGTATG GCATTGTGTG GCAACTCTAA AGTTGTCATG 
CTTGATGAAC CCACTGCCGG AATGGATCCA TCAGCAAGAC GAGCTTTGTG GGATTTGCTT 
CAAAATCAAA AACACGGAAG GACGATGCTT TTAACAACAC ATTTTATGGA CGAAGCTGAT 
TTATTAGGTG ATAGAATCGC TATTATGGCC GGAGGGGAGT TACAGTGTTG CGGCTCAAGT 
TTCTTCCTAA AGAAGAAATA CGGGACGGGA TACCATCTCA TAATGGATAA GGCTTCGAAT 
TGTCAAGAGG AACAAGTAAC ACAACTTTTG AAGCGACATA TTCCTAATAT CCAGGTACAC 
GGTAATGTTG GCTCTGAACT CACGTATCTA CTATCCGAAG ATCACTCTTC AGTTTTTGAA 
GAAATGCTGC GAGACTTAGA AGAAAATAGT GATAGTTTGG GTATTCGTAG TTACGGAATT 
TCACTAACAA CTTTAGAAGA AGTTTTCATG AAAGTCGGTG CTGACCACGG CCAGGAAGAA 
AGCTATAACG GTTTTGTGCA AGAAACAGAT TGTAATAAAA CAATGCTGGG TAAAATAAAA 
TCGAAATTGA AAAGGAATGA AAACTACGAA AATAGCATTA ACGAAACAAC TTTAAACCTT 
GAAACTTCTG CTCTTCTAAC CGGTTTCATG TTAATCAAAA ATCAGTTTTT GGCAATGCTT 
ATGAAAAAAA TTTTATCAAT CGTACGAACC TGGATATTGC AGTTTATTCA AATTTTGATG 
CCAGTTGCGT TTCTTATCAT AGCCATTGTC GTTAGCAGAA ACACTAACAA ATCAGCAGAT 
TTACCCAAAC TGCCCATAAC ATTAGATTCG TATCGCAATC CAGTAACGCT AATTGAAGAT 
AATGCAAATA ACTCATATTC GGCCTTGTAT ATGCAAAGCC TTAAAGGTTA TGAAATTCAA 
AGAGTGGCAA GCATCCACGA TGTAATGCTT AATTTGACCG TAACTGTTCC ATCCACTGTC 
AGACTAAGAT ACATTGTTGG AGCTAGTTTC AACAAAACAT TCGGCGGTGT TTTTACAACT 
ATCACAGCCT GGTTTAATAA TAACCCTTAC CACTCCCCGC CTCTAGCATT AGGTCTTGCC 
TTAAACGTGG CTTTCAAGCA TTTAGCTGGC ACAAATCGTT CAATAAGATG GGCAAATTAT 
CCGTTACCGT TTACAGCCGA TACAAAAGTG CAGCAACTGT TGGAAGGCAA CAGTATGGGT 
TTCCAGCTCG CTTTCAACAT CGGATTTAGT ATGGCTTTTG TATCCTCATT CTACGTCCTA 
TTTTATGTGC GGGAACGAGT TTGCAAGTCA AAACATTTAC AATTCGTGTC CGGAGTGAAA 
GTTTACATAT TCTGGATAAT GTCATTCGTC TGTGATATAA TCACGTTCAT TGTGACCATC 
ATCGCGATCA TCATTACTTT GGCGTGTTTC CAAGAAGATG GCTTCCGGTC GGCTGAGGAC 
TTAGGTCGCA TGTTCCTTCT TTTGTTCTAT TTTGGCGTTG CAATGTTGCC TATGATGTAC 
TTTGCGTCAT ACTTGTTCGA AGTACCTTCG ACAGGTTATA CTAGAATGAC CCTCTTTAGT 
GTTTTCACTG GAGTGGCTGC CTTTTTGGTT GTTCAAGTTT TGGCAACACC TGGATTGGAT 
TTGGAATATG TCGCGAATAC TCTACATTGG ATTTTCCTGG TAGTACCCCA TTATTCGTTA 
GCTACAGGTA TTAGAGACGT TTATACAACA TTTGCCACTA ATAAAATGTG CAAATACGCC 
GTCCAAACGT GCGTCGAGAA TAGGCCAGAT ATGACTGTGG ACAAGTGTTG GGATTTGACT 
TGTCATAATG CAAGTGCTGT GCTACAGGAT TACTGTTGTG TACGCGAGCC GCACTATTAC 
AAATGGGCCA GTCCTGGAAT AGGCCGAAAT TTGCTATACT CATTTTCCGT TGGAATAGTC 
CTATTTATTC TACTCAACAT AAAAGAATAT AAAATATTTT CAAAAACGAT AGACCATATT 
GTTGAGAGCT ATAGAAACAA ATTGCCCCAG CCAGTGGAAA ATGAAGACAG CGATGTCGCG 
GAAGAAAAAC ACAAAATCCA CAACACTTCT GAAACAGAGT TACAAAAAGA CTACATTTTG 
GTGCTTAAAG ATTTGACAAA GTATTACAAC AACTTTTTGG CAGTTAACGG ATTGTGTCTG 
GGTGTTAAGG AACGTGAATG CTTCGGATTA TTGGGTATAA ACGGTGCTGG GAAAACCACA 
ACTTTTAAAA TGATGACCGG TGATGTAAAA ATCACGCACG GAGAAGCTTG GGTGAAAAAA 
TTCAGTATCA AATCGCAACT AAAACAAGTG CAAAAGTTGA TTGGATATTG TCCGCAATTT 
GACGCTCTTC TTGATGACCT GACTGCCAAA GAATCATTGG TAATGTTTGC ACTTTTGAGA 
GGAGTTCCAA TGGACGAGTG CCGATACCTG GTCCAGAAAC TATCGACAGA TTTTGATTTC 
CAAAGACATT TGAATAAAAA GGTTAAGGAA CTTAGTGGTG GTAACAAGAG GAAGTTAAGT 
ACTGCCATTT CGCTGATTGG CGATCCTCCG GTGATATATT TAGACGAACC TACGACAGGA 
ATGGACCCTG CGACGAAACG CTATTTGTGG GATGCCATCT GCAAAGTTAG AGATAACGGA 
AAATGTGTAG TCTTGACCTC TCATAGTATG GAAGAATGTG AAGCTTTGTG CACAAGACTA 
GCCATAATGG TTAATGGCAA TTTCAAATGT TTGGGTTCCA CTCAACATTT GAAAAATAAA 
TTTGCCGAAG GGTATACGTT GACAATTAAA ATTAAGAAAA GCCCTGGCGG GGAAGATGCC 
GATATTAAAC CAATTGAAAA TTTCATCTCG GACCATTTTC CCAAAGCAAT ACAAAGAGAA 
AAACATCAAG AGCTTTTAAC ATATTATATT GTAGATAAAA GCATCCCCTG GTCCAGAATG 
TTCGGGATTT TGGAAAAAGG AAAAAAGCAG CTAAATATTG AAGATTATTC TTTGGGTCAA 
TCAAGTTTGG AACAGGTATT TTTATCTTTC ACAAGGTTGC AACGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC659344 ( XM_008200928.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008199150.1 Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X2, mRNA.

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CloneID	OTg14532
Clone ID Related Accession (Same CDS sequence)	XM_008200928.2
Accession Version	XM_008200928.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	ATP-binding cassette sub-family A member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015451937.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008200928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGTCA GTTCGAGAAC AATGGGGAAG AAACTGGACA AGTTCCTTCT ACTGATGTGG 
AAGAATTGGT TGCTGCAATA CCGGAAGCCT CTCCAAACCC TTGTAGAAAT TCTAGCTCCA 
GTCCTGTTCT CCATCCTCCT AGTCGTTATT CGAAGTCTGG TTGATCCTGA AACGAATGAT 
CCAAAGATTT ACACGCCCTT CTGTCTACTC CCAATTTTAT GCTCGGATGA AAGTATTAGC 
ACAAATCCGA AATTTCTAAC GAACCTCGGA AATACGACGT TCAGAAACTT TACACTAGTG 
TATTCACCTT ACTCAAACAA AGCCGTAAGA CGGTCAATGG GAATTCTTGA AACTGTTTTG 
GGTGGCCACG TGGTTGGTTA CAACAACTCT GACCAACTAG AAGCCCATTT CCTCGATTCG 
AATTCCTCTC TCACGTTTGC TGCCATCCAA CTTGATGACT CTTTAGCGCA CCAAGACACT 
CTACCAGACG ACTTGGAAGT ATCGCTGAGA TTTCCAAGCG AACTTCGGAT TGGGTTCGAT 
TTGTTTGGGC AAAACTGGCA CACAAATCTT TTGTATCCTG TGTATCAAGT ACCAGGCCCC 
CGAGCGCCAG AGAACAACAC AGGGGCTCTC CCAGAAAAGT TACGCGATTT AGATAGAAGA 
AAAAGAACGT GGAGAACTAA TCTTTTATTT CCCATTTATC AATCACCTGG TCCTAGATCC 
CCAAGATCAG ACATTGGTGG TAAACCAGGT TACTTCAAAG AAGGATTCTT GACCTTACAA 
CTAGTCATGA CGTACATTTT TGCAATTGGC GGACACCTTG AAGCGCAAAA TCTAACACAG 
TTTCAAAATA TTATTACTAC ACTTCTGCCA CCGCTAATTA AAATGCAACG TTTTCCGTAT 
GGAAGTTGGA TAGAGGATCC TTTGCTACAA GCTTTAGAAA GTTTCGTTGG AGTTATTATT 
ATGTTGAGTT TCGTTTACAC TTGCATAAAC ACGGTTAAAG TTATAACAAC CGAAAAAGAG 
AAACAGCTTA AGGAGGCTAT GAAAATTATG GGTTTGCCTA ACTGGCTCCA TTGGACTGCG 
TGGTTTATCA AAACATTTGT CTTTCTTTTA GTTTCAGTAG TTTTAATTAT TTTACTTTTG 
AAAATCTCGT GGTATTCACA CACCGAATAT AGTGTTTTCA CGTACGCAGA TCCCACAGTA 
ATGTTGGTGT TCTTAGTACT GTACGTTTGT GCAACTATAA CTTTTTGTTT TGCGATTAGC 
GTATTTTTTT CTAAAGCAAA TACAGCTGCA ACAGTAGCTG GTCTTGTATG GTTCCTCTCT 
TATGCTCCAT ATTTATTTTT ACAAAAGCAG TATGACCAAC TCAGTCTCTC TACTAAACTC 
ATAGCAAGTC TTGGGTCAAA CACTGCAATG GCTTACGGGT TCCAGTTAAT GTTAATGTAT 
GAGGGTACCA GTGAAGGAAT ACAATGGTCC AATATAGGAA GACCCAATAC TCCCGATGAC 
AGTCTAACGC TCGGTTACAT AATGATGATG CTGGTAATAG ACACTTTTAT CTATCTTTTT 
GTGGCTCTTT ACGTCGAAGC CGTTTTTCCC GGGGAGTACG GAGTGCCACT TCCTTGGTAC 
TTCCCGTTTA CTTCCGCCTA TTGGTGTGGT CATCCTAGCT ATGTAGGAGT TGAAGATTTC 
ACTAGTGTCC CATCACAAGA AGGTGAATTT TTTGAAAAAG AACCTAACAA TCTTACACCC 
GGTATACAAA TTCGAAATTT GCGGAAAGTT TTTGACCAAA AAGCAGCAGT TCGAAATCTC 
TCGCTAAACA TGTACAACGA TCAAATTACG GTCCTTCTTG GACACAATGG TGCCGGAAAA 
ACTACCACCA TGTCCATGCT GACTGGAATG TTTCCTCCCA CAAGTGGTAC TGCAGTCGTT 
TGTGGACACG ACATTAAAAC GGATATGGAC GGAGTAAGAG AAAGTCTGGG TCTATGTCCT 
CAACATAACA TCATCTTCGA CGAACTCACC GTCCGAGAGC ATCTCTACTT TTTCAGTAAA 
CTTAAAGGAC TGAAACGTGA AGCAATCAAT GAAGAAATTG ATAAATACGT CGAACTGTTG 
GAACTGCAAC CTAAAGCGAA TGCAAAATCG GCCACTTTGT CAGGAGGAAT GAAGAGAAAA 
CTTTGTGTGG GTATGGCATT GTGTGGCAAC TCTAAAGTTG TCATGCTTGA TGAACCCACT 
GCCGGAATGG ATCCATCAGC AAGACGAGCT TTGTGGGATT TGCTTCAAAA TCAAAAACAC 
GGAAGGACGA TGCTTTTAAC AACACATTTT ATGGACGAAG CTGATTTATT AGGTGATAGA 
ATCGCTATTA TGGCCGGAGG GGAGTTACAG TGTTGCGGCT CAAGTTTCTT CCTAAAGAAG 
AAATACGGGA CGGGATACCA TCTCATAATG GATAAGGCTT CGAATTGTCA AGAGGAACAA 
GTAACACAAC TTTTGAAGCG ACATATTCCT AATATCCAGG TACACGGTAA TGTTGGCTCT 
GAACTCACGT ATCTACTATC CGAAGATCAC TCTTCAGTTT TTGAAGAAAT GCTGCGAGAC 
TTAGAAGAAA ATAGTGATAG TTTGGGTATT CGTAGTTACG GAATTTCACT AACAACTTTA 
GAAGAAGTTT TCATGAAAGT CGGTGCTGAC CACGGCCAGG AAGAAAGCTA TAACGGTTTT 
GTGCAAGAAA CAGATTGTAA TAAAACAATG CTGGGTAAAA TAAAATCGAA ATTGAAAAGG 
AATGAAAACT ACGAAAATAG CATTAACGAA ACAACTTTAA ACCTTGAAAC TTCTGCTCTT 
CTAACCGGTT TCATGTTAAT CAAAAATCAG TTTTTGGCAA TGCTTATGAA AAAAATTTTA 
TCAATCGTAC GAACCTGGAT ATTGCAGTTT ATTCAAATTT TGATGCCAGT TGCGTTTCTT 
ATCATAGCCA TTGTCGTTAG CAGAAACACT AACAAATCAG CAGATTTACC CAAACTGCCC 
ATAACATTAG ATTCGTATCG CAATCCAGTA ACGCTAATTG AAGATAATGC AAATAACTCA 
TATTCGGCCT TGTATATGCA AAGCCTTAAA GGTTATGAAA TTCAAAGAGT GGCAAGCATC 
CACGATGTAA TGCTTAATTT GACCGTAACT GTTCCATCCA CTGTCAGACT AAGATACATT 
GTTGGAGCTA GTTTCAACAA AACATTCGGC GGTGTTTTTA CAACTATCAC AGCCTGGTTT 
AATAATAACC CTTACCACTC CCCGCCTCTA GCATTAGGTC TTGCCTTAAA CGTGGCTTTC 
AAGCATTTAG CTGGCACAAA TCGTTCAATA AGATGGGCAA ATTATCCGTT ACCGTTTACA 
GCCGATACAA AAGTGCAGCA ACTGTTGGAA GGCAACAGTA TGGGTTTCCA GCTCGCTTTC 
AACATCGGAT TTAGTATGGC TTTTGTATCC TCATTCTACG TCCTATTTTA TGTGCGGGAA 
CGAGTTTGCA AGTCAAAACA TTTACAATTC GTGTCCGGAG TGAAAGTTTA CATATTCTGG 
ATAATGTCAT TCGTCTGTGA TATAATCACG TTCATTGTGA CCATCATCGC GATCATCATT 
ACTTTGGCGT GTTTCCAAGA AGATGGCTTC CGGTCGGCTG AGGACTTAGG TCGCATGTTC 
CTTCTTTTGT TCTATTTTGG CGTTGCAATG TTGCCTATGA TGTACTTTGC GTCATACTTG 
TTCGAAGTAC CTTCGACAGG TTATACTAGA ATGACCCTCT TTAGTGTTTT CACTGGAGTG 
GCTGCCTTTT TGGTTGTTCA AGTTTTGGCA ACACCTGGAT TGGATTTGGA ATATGTCGCG 
AATACTCTAC ATTGGATTTT CCTGGTAGTA CCCCATTATT CGTTAGCTAC AGGTATTAGA 
GACGTTTATA CAACATTTGC CACTAATAAA ATGTGCAAAT ACGCCGTCCA AACGTGCGTC 
GAGAATAGGC CAGATATGAC TGTGGACAAG TGTTGGGATT TGACTTGTCA TAATGCAAGT 
GCTGTGCTAC AGGATTACTG TTGTGTACGC GAGCCGCACT ATTACAAATG GGCCAGTCCT 
GGAATAGGCC GAAATTTGCT ATACTCATTT TCCGTTGGAA TAGTCCTATT TATTCTACTC 
AACATAAAAG AATATAAAAT ATTTTCAAAA ACGATAGACC ATATTGTTGA GAGCTATAGA 
AACAAATTGC CCCAGCCAGT GGAAAATGAA GACAGCGATG TCGCGGAAGA AAAACACAAA 
ATCCACAACA CTTCTGAAAC AGAGTTACAA AAAGACTACA TTTTGGTGCT TAAAGATTTG 
ACAAAGTATT ACAACAACTT TTTGGCAGTT AACGGATTGT GTCTGGGTGT TAAGGAACGT 
GAATGCTTCG GATTATTGGG TATAAACGGT GCTGGGAAAA CCACAACTTT TAAAATGATG 
ACCGGTGATG TAAAAATCAC GCACGGAGAA GCTTGGGTGA AAAAATTCAG TATCAAATCG 
CAACTAAAAC AAGTGCAAAA GTTGATTGGA TATTGTCCGC AATTTGACGC TCTTCTTGAT 
GACCTGACTG CCAAAGAATC ATTGGTAATG TTTGCACTTT TGAGAGGAGT TCCAATGGAC 
GAGTGCCGAT ACCTGGTCCA GAAACTATCG ACAGATTTTG ATTTCCAAAG ACATTTGAAT 
AAAAAGGTTA AGGAACTTAG TGGTGGTAAC AAGAGGAAGT TAAGTACTGC CATTTCGCTG 
ATTGGCGATC CTCCGGTGAT ATATTTAGAC GAACCTACGA CAGGAATGGA CCCTGCGACG 
AAACGCTATT TGTGGGATGC CATCTGCAAA GTTAGAGATA ACGGAAAATG TGTAGTCTTG 
ACCTCTCATA GTATGGAAGA ATGTGAAGCT TTGTGCACAA GACTAGCCAT AATGGTTAAT 
GGCAATTTCA AATGTTTGGG TTCCACTCAA CATTTGAAAA ATAAATTTGC CGAAGGGTAT 
ACGTTGACAA TTAAAATTAA GAAAAGCCCT GGCGGGGAAG ATGCCGATAT TAAACCAATT 
GAAAATTTCA TCTCGGACCA TTTTCCCAAA GCAATACAAA GAGAAAAACA TCAAGAGCTT 
TTAACATATT ATATTGTAGA TAAAAGCATC CCCTGGTCCA GAATGTTCGG GATTTTGGAA 
AAAGGAAAAA AGCAGCTAAA TATTGAAGAT TATTCTTTGG GTCAATCAAG TTTGGAACAG 
GTATTTTTAT CTTTCACAAG GTTGCAACGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008199150.1
CDS	85..5337
Translation	MYVSSRTMGKKLDKFLLLMWKNWLLQYRKPLQTLVEILAPVLFSILLVVIRSLVDPETNDPKIYTPFCLLPILCSDESISTNPKFLTNLGNTTFRNFTLVYSPYSNKAVRRSMGILETVLGGHVVGYNNSDQLEAHFLDSNSSLTFAAIQLDDSLAHQDTLPDDLEVSLRFPSELRIGFDLFGQNWHTNLLYPVYQVPGPRAPENNTGALPEKLRDLDRRKRTWRTNLLFPIYQSPGPRSPRSDIGGKPGYFKEGFLTLQLVMTYIFAIGGHLEAQNLTQFQNIITTLLPPLIKMQRFPYGSWIEDPLLQALESFVGVIIMLSFVYTCINTVKVITTEKEKQLKEAMKIMGLPNWLHWTAWFIKTFVFLLVSVVLIILLLKISWYSHTEYSVFTYADPTVMLVFLVLYVCATITFCFAISVFFSKANTAATVAGLVWFLSYAPYLFLQKQYDQLSLSTKLIASLGSNTAMAYGFQLMLMYEGTSEGIQWSNIGRPNTPDDSLTLGYIMMMLVIDTFIYLFVALYVEAVFPGEYGVPLPWYFPFTSAYWCGHPSYVGVEDFTSVPSQEGEFFEKEPNNLTPGIQIRNLRKVFDQKAAVRNLSLNMYNDQITVLLGHNGAGKTTTMSMLTGMFPPTSGTAVVCGHDIKTDMDGVRESLGLCPQHNIIFDELTVREHLYFFSKLKGLKREAINEEIDKYVELLELQPKANAKSATLSGGMKRKLCVGMALCGNSKVVMLDEPTAGMDPSARRALWDLLQNQKHGRTMLLTTHFMDEADLLGDRIAIMAGGELQCCGSSFFLKKKYGTGYHLIMDKASNCQEEQVTQLLKRHIPNIQVHGNVGSELTYLLSEDHSSVFEEMLRDLEENSDSLGIRSYGISLTTLEEVFMKVGADHGQEESYNGFVQETDCNKTMLGKIKSKLKRNENYENSINETTLNLETSALLTGFMLIKNQFLAMLMKKILSIVRTWILQFIQILMPVAFLIIAIVVSRNTNKSADLPKLPITLDSYRNPVTLIEDNANNSYSALYMQSLKGYEIQRVASIHDVMLNLTVTVPSTVRLRYIVGASFNKTFGGVFTTITAWFNNNPYHSPPLALGLALNVAFKHLAGTNRSIRWANYPLPFTADTKVQQLLEGNSMGFQLAFNIGFSMAFVSSFYVLFYVRERVCKSKHLQFVSGVKVYIFWIMSFVCDIITFIVTIIAIIITLACFQEDGFRSAEDLGRMFLLLFYFGVAMLPMMYFASYLFEVPSTGYTRMTLFSVFTGVAAFLVVQVLATPGLDLEYVANTLHWIFLVVPHYSLATGIRDVYTTFATNKMCKYAVQTCVENRPDMTVDKCWDLTCHNASAVLQDYCCVREPHYYKWASPGIGRNLLYSFSVGIVLFILLNIKEYKIFSKTIDHIVESYRNKLPQPVENEDSDVAEEKHKIHNTSETELQKDYILVLKDLTKYYNNFLAVNGLCLGVKERECFGLLGINGAGKTTTFKMMTGDVKITHGEAWVKKFSIKSQLKQVQKLIGYCPQFDALLDDLTAKESLVMFALLRGVPMDECRYLVQKLSTDFDFQRHLNKKVKELSGGNKRKLSTAISLIGDPPVIYLDEPTTGMDPATKRYLWDAICKVRDNGKCVVLTSHSMEECEALCTRLAIMVNGNFKCLGSTQHLKNKFAEGYTLTIKIKKSPGGEDADIKPIENFISDHFPKAIQREKHQELLTYYIVDKSIPWSRMFGILEKGKKQLNIEDYSLGQSSLEQVFLSFTRLQR

Target ORF information:

RefSeq Version	XM_008200928.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008200928.2

ORF Insert Sequence:

ATGTATGTCA GTTCGAGAAC AATGGGGAAG AAACTGGACA AGTTCCTTCT ACTGATGTGG 
AAGAATTGGT TGCTGCAATA CCGGAAGCCT CTCCAAACCC TTGTAGAAAT TCTAGCTCCA 
GTCCTGTTCT CCATCCTCCT AGTCGTTATT CGAAGTCTGG TTGATCCTGA AACGAATGAT 
CCAAAGATTT ACACGCCCTT CTGTCTACTC CCAATTTTAT GCTCGGATGA AAGTATTAGC 
ACAAATCCGA AATTTCTAAC GAACCTCGGA AATACGACGT TCAGAAACTT TACACTAGTG 
TATTCACCTT ACTCAAACAA AGCCGTAAGA CGGTCAATGG GAATTCTTGA AACTGTTTTG 
GGTGGCCACG TGGTTGGTTA CAACAACTCT GACCAACTAG AAGCCCATTT CCTCGATTCG 
AATTCCTCTC TCACGTTTGC TGCCATCCAA CTTGATGACT CTTTAGCGCA CCAAGACACT 
CTACCAGACG ACTTGGAAGT ATCGCTGAGA TTTCCAAGCG AACTTCGGAT TGGGTTCGAT 
TTGTTTGGGC AAAACTGGCA CACAAATCTT TTGTATCCTG TGTATCAAGT ACCAGGCCCC 
CGAGCGCCAG AGAACAACAC AGGGGCTCTC CCAGAAAAGT TACGCGATTT AGATAGAAGA 
AAAAGAACGT GGAGAACTAA TCTTTTATTT CCCATTTATC AATCACCTGG TCCTAGATCC 
CCAAGATCAG ACATTGGTGG TAAACCAGGT TACTTCAAAG AAGGATTCTT GACCTTACAA 
CTAGTCATGA CGTACATTTT TGCAATTGGC GGACACCTTG AAGCGCAAAA TCTAACACAG 
TTTCAAAATA TTATTACTAC ACTTCTGCCA CCGCTAATTA AAATGCAACG TTTTCCGTAT 
GGAAGTTGGA TAGAGGATCC TTTGCTACAA GCTTTAGAAA GTTTCGTTGG AGTTATTATT 
ATGTTGAGTT TCGTTTACAC TTGCATAAAC ACGGTTAAAG TTATAACAAC CGAAAAAGAG 
AAACAGCTTA AGGAGGCTAT GAAAATTATG GGTTTGCCTA ACTGGCTCCA TTGGACTGCG 
TGGTTTATCA AAACATTTGT CTTTCTTTTA GTTTCAGTAG TTTTAATTAT TTTACTTTTG 
AAAATCTCGT GGTATTCACA CACCGAATAT AGTGTTTTCA CGTACGCAGA TCCCACAGTA 
ATGTTGGTGT TCTTAGTACT GTACGTTTGT GCAACTATAA CTTTTTGTTT TGCGATTAGC 
GTATTTTTTT CTAAAGCAAA TACAGCTGCA ACAGTAGCTG GTCTTGTATG GTTCCTCTCT 
TATGCTCCAT ATTTATTTTT ACAAAAGCAG TATGACCAAC TCAGTCTCTC TACTAAACTC 
ATAGCAAGTC TTGGGTCAAA CACTGCAATG GCTTACGGGT TCCAGTTAAT GTTAATGTAT 
GAGGGTACCA GTGAAGGAAT ACAATGGTCC AATATAGGAA GACCCAATAC TCCCGATGAC 
AGTCTAACGC TCGGTTACAT AATGATGATG CTGGTAATAG ACACTTTTAT CTATCTTTTT 
GTGGCTCTTT ACGTCGAAGC CGTTTTTCCC GGGGAGTACG GAGTGCCACT TCCTTGGTAC 
TTCCCGTTTA CTTCCGCCTA TTGGTGTGGT CATCCTAGCT ATGTAGGAGT TGAAGATTTC 
ACTAGTGTCC CATCACAAGA AGGTGAATTT TTTGAAAAAG AACCTAACAA TCTTACACCC 
GGTATACAAA TTCGAAATTT GCGGAAAGTT TTTGACCAAA AAGCAGCAGT TCGAAATCTC 
TCGCTAAACA TGTACAACGA TCAAATTACG GTCCTTCTTG GACACAATGG TGCCGGAAAA 
ACTACCACCA TGTCCATGCT GACTGGAATG TTTCCTCCCA CAAGTGGTAC TGCAGTCGTT 
TGTGGACACG ACATTAAAAC GGATATGGAC GGAGTAAGAG AAAGTCTGGG TCTATGTCCT 
CAACATAACA TCATCTTCGA CGAACTCACC GTCCGAGAGC ATCTCTACTT TTTCAGTAAA 
CTTAAAGGAC TGAAACGTGA AGCAATCAAT GAAGAAATTG ATAAATACGT CGAACTGTTG 
GAACTGCAAC CTAAAGCGAA TGCAAAATCG GCCACTTTGT CAGGAGGAAT GAAGAGAAAA 
CTTTGTGTGG GTATGGCATT GTGTGGCAAC TCTAAAGTTG TCATGCTTGA TGAACCCACT 
GCCGGAATGG ATCCATCAGC AAGACGAGCT TTGTGGGATT TGCTTCAAAA TCAAAAACAC 
GGAAGGACGA TGCTTTTAAC AACACATTTT ATGGACGAAG CTGATTTATT AGGTGATAGA 
ATCGCTATTA TGGCCGGAGG GGAGTTACAG TGTTGCGGCT CAAGTTTCTT CCTAAAGAAG 
AAATACGGGA CGGGATACCA TCTCATAATG GATAAGGCTT CGAATTGTCA AGAGGAACAA 
GTAACACAAC TTTTGAAGCG ACATATTCCT AATATCCAGG TACACGGTAA TGTTGGCTCT 
GAACTCACGT ATCTACTATC CGAAGATCAC TCTTCAGTTT TTGAAGAAAT GCTGCGAGAC 
TTAGAAGAAA ATAGTGATAG TTTGGGTATT CGTAGTTACG GAATTTCACT AACAACTTTA 
GAAGAAGTTT TCATGAAAGT CGGTGCTGAC CACGGCCAGG AAGAAAGCTA TAACGGTTTT 
GTGCAAGAAA CAGATTGTAA TAAAACAATG CTGGGTAAAA TAAAATCGAA ATTGAAAAGG 
AATGAAAACT ACGAAAATAG CATTAACGAA ACAACTTTAA ACCTTGAAAC TTCTGCTCTT 
CTAACCGGTT TCATGTTAAT CAAAAATCAG TTTTTGGCAA TGCTTATGAA AAAAATTTTA 
TCAATCGTAC GAACCTGGAT ATTGCAGTTT ATTCAAATTT TGATGCCAGT TGCGTTTCTT 
ATCATAGCCA TTGTCGTTAG CAGAAACACT AACAAATCAG CAGATTTACC CAAACTGCCC 
ATAACATTAG ATTCGTATCG CAATCCAGTA ACGCTAATTG AAGATAATGC AAATAACTCA 
TATTCGGCCT TGTATATGCA AAGCCTTAAA GGTTATGAAA TTCAAAGAGT GGCAAGCATC 
CACGATGTAA TGCTTAATTT GACCGTAACT GTTCCATCCA CTGTCAGACT AAGATACATT 
GTTGGAGCTA GTTTCAACAA AACATTCGGC GGTGTTTTTA CAACTATCAC AGCCTGGTTT 
AATAATAACC CTTACCACTC CCCGCCTCTA GCATTAGGTC TTGCCTTAAA CGTGGCTTTC 
AAGCATTTAG CTGGCACAAA TCGTTCAATA AGATGGGCAA ATTATCCGTT ACCGTTTACA 
GCCGATACAA AAGTGCAGCA ACTGTTGGAA GGCAACAGTA TGGGTTTCCA GCTCGCTTTC 
AACATCGGAT TTAGTATGGC TTTTGTATCC TCATTCTACG TCCTATTTTA TGTGCGGGAA 
CGAGTTTGCA AGTCAAAACA TTTACAATTC GTGTCCGGAG TGAAAGTTTA CATATTCTGG 
ATAATGTCAT TCGTCTGTGA TATAATCACG TTCATTGTGA CCATCATCGC GATCATCATT 
ACTTTGGCGT GTTTCCAAGA AGATGGCTTC CGGTCGGCTG AGGACTTAGG TCGCATGTTC 
CTTCTTTTGT TCTATTTTGG CGTTGCAATG TTGCCTATGA TGTACTTTGC GTCATACTTG 
TTCGAAGTAC CTTCGACAGG TTATACTAGA ATGACCCTCT TTAGTGTTTT CACTGGAGTG 
GCTGCCTTTT TGGTTGTTCA AGTTTTGGCA ACACCTGGAT TGGATTTGGA ATATGTCGCG 
AATACTCTAC ATTGGATTTT CCTGGTAGTA CCCCATTATT CGTTAGCTAC AGGTATTAGA 
GACGTTTATA CAACATTTGC CACTAATAAA ATGTGCAAAT ACGCCGTCCA AACGTGCGTC 
GAGAATAGGC CAGATATGAC TGTGGACAAG TGTTGGGATT TGACTTGTCA TAATGCAAGT 
GCTGTGCTAC AGGATTACTG TTGTGTACGC GAGCCGCACT ATTACAAATG GGCCAGTCCT 
GGAATAGGCC GAAATTTGCT ATACTCATTT TCCGTTGGAA TAGTCCTATT TATTCTACTC 
AACATAAAAG AATATAAAAT ATTTTCAAAA ACGATAGACC ATATTGTTGA GAGCTATAGA 
AACAAATTGC CCCAGCCAGT GGAAAATGAA GACAGCGATG TCGCGGAAGA AAAACACAAA 
ATCCACAACA CTTCTGAAAC AGAGTTACAA AAAGACTACA TTTTGGTGCT TAAAGATTTG 
ACAAAGTATT ACAACAACTT TTTGGCAGTT AACGGATTGT GTCTGGGTGT TAAGGAACGT 
GAATGCTTCG GATTATTGGG TATAAACGGT GCTGGGAAAA CCACAACTTT TAAAATGATG 
ACCGGTGATG TAAAAATCAC GCACGGAGAA GCTTGGGTGA AAAAATTCAG TATCAAATCG 
CAACTAAAAC AAGTGCAAAA GTTGATTGGA TATTGTCCGC AATTTGACGC TCTTCTTGAT 
GACCTGACTG CCAAAGAATC ATTGGTAATG TTTGCACTTT TGAGAGGAGT TCCAATGGAC 
GAGTGCCGAT ACCTGGTCCA GAAACTATCG ACAGATTTTG ATTTCCAAAG ACATTTGAAT 
AAAAAGGTTA AGGAACTTAG TGGTGGTAAC AAGAGGAAGT TAAGTACTGC CATTTCGCTG 
ATTGGCGATC CTCCGGTGAT ATATTTAGAC GAACCTACGA CAGGAATGGA CCCTGCGACG 
AAACGCTATT TGTGGGATGC CATCTGCAAA GTTAGAGATA ACGGAAAATG TGTAGTCTTG 
ACCTCTCATA GTATGGAAGA ATGTGAAGCT TTGTGCACAA GACTAGCCAT AATGGTTAAT 
GGCAATTTCA AATGTTTGGG TTCCACTCAA CATTTGAAAA ATAAATTTGC CGAAGGGTAT 
ACGTTGACAA TTAAAATTAA GAAAAGCCCT GGCGGGGAAG ATGCCGATAT TAAACCAATT 
GAAAATTTCA TCTCGGACCA TTTTCCCAAA GCAATACAAA GAGAAAAACA TCAAGAGCTT 
TTAACATATT ATATTGTAGA TAAAAGCATC CCCTGGTCCA GAATGTTCGG GATTTTGGAA 
AAAGGAAAAA AGCAGCTAAA TATTGAAGAT TATTCTTTGG GTCAATCAAG TTTGGAACAG 
GTATTTTTAT CTTTCACAAG GTTGCAACGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC659344 ( XM_015984873.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015840359.1 Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OTg14533
Clone ID Related Accession (Same CDS sequence)	XM_015984873.1 , XM_008200929.2 , XM_015984872.1 , XM_008200930.2
Accession Version	XM_015984873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	ATP-binding cassette sub-family A member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015451937.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AACTGGACAA GTTCCTTCTA CTGATGTGGA AGAATTGGTT GCTGCAATAC 
CGGAAGCCTC TCCAAACCCT TGTAGAAATT CTAGCTCCAG TCCTGTTCTC CATCCTCCTA 
GTCGTTATTC GAAGTCTGGT TGATCCTGAA ACGAATGATC CAAAGATTTA CACGCCCTTC 
TGTCTACTCC CAATTTTATG CTCGGATGAA AGTATTAGCA CAAATCCGAA ATTTCTAACG 
AACCTCGGAA ATACGACGTT CAGAAACTTT ACACTAGTGT ATTCACCTTA CTCAAACAAA 
GCCGTAAGAC GGTCAATGGG AATTCTTGAA ACTGTTTTGG GTGGCCACGT GGTTGGTTAC 
AACAACTCTG ACCAACTAGA AGCCCATTTC CTCGATTCGA ATTCCTCTCT CACGTTTGCT 
GCCATCCAAC TTGATGACTC TTTAGCGCAC CAAGACACTC TACCAGACGA CTTGGAAGTA 
TCGCTGAGAT TTCCAAGCGA ACTTCGGATT GGGTTCGATT TGTTTGGGCA AAACTGGCAC 
ACAAATCTTT TGTATCCTGT GTATCAAGTA CCAGGCCCCC GAGCGCCAGA GAACAACACA 
GGGGCTCTCC CAGAAAAGTT ACGCGATTTA GATAGAAGAA AAAGAACGTG GAGAACTAAT 
CTTTTATTTC CCATTTATCA ATCACCTGGT CCTAGATCCC CAAGATCAGA CATTGGTGGT 
AAACCAGGTT ACTTCAAAGA AGGATTCTTG ACCTTACAAC TAGTCATGAC GTACATTTTT 
GCAATTGGCG GACACCTTGA AGCGCAAAAT CTAACACAGT TTCAAAATAT TATTACTACA 
CTTCTGCCAC CGCTAATTAA AATGCAACGT TTTCCGTATG GAAGTTGGAT AGAGGATCCT 
TTGCTACAAG CTTTAGAAAG TTTCGTTGGA GTTATTATTA TGTTGAGTTT CGTTTACACT 
TGCATAAACA CGGTTAAAGT TATAACAACC GAAAAAGAGA AACAGCTTAA GGAGGCTATG 
AAAATTATGG GTTTGCCTAA CTGGCTCCAT TGGACTGCGT GGTTTATCAA AACATTTGTC 
TTTCTTTTAG TTTCAGTAGT TTTAATTATT TTACTTTTGA AAATCTCGTG GTATTCACAC 
ACCGAATATA GTGTTTTCAC GTACGCAGAT CCCACAGTAA TGTTGGTGTT CTTAGTACTG 
TACGTTTGTG CAACTATAAC TTTTTGTTTT GCGATTAGCG TATTTTTTTC TAAAGCAAAT 
ACAGCTGCAA CAGTAGCTGG TCTTGTATGG TTCCTCTCTT ATGCTCCATA TTTATTTTTA 
CAAAAGCAGT ATGACCAACT CAGTCTCTCT ACTAAACTCA TAGCAAGTCT TGGGTCAAAC 
ACTGCAATGG CTTACGGGTT CCAGTTAATG TTAATGTATG AGGGTACCAG TGAAGGAATA 
CAATGGTCCA ATATAGGAAG ACCCAATACT CCCGATGACA GTCTAACGCT CGGTTACATA 
ATGATGATGC TGGTAATAGA CACTTTTATC TATCTTTTTG TGGCTCTTTA CGTCGAAGCC 
GTTTTTCCCG GGGAGTACGG AGTGCCACTT CCTTGGTACT TCCCGTTTAC TTCCGCCTAT 
TGGTGTGGTC ATCCTAGCTA TGTAGGAGTT GAAGATTTCA CTAGTGTCCC ATCACAAGAA 
GGTGAATTTT TTGAAAAAGA ACCTAACAAT CTTACACCCG GTATACAAAT TCGAAATTTG 
CGGAAAGTTT TTGACCAAAA AGCAGCAGTT CGAAATCTCT CGCTAAACAT GTACAACGAT 
CAAATTACGG TCCTTCTTGG ACACAATGGT GCCGGAAAAA CTACCACCAT GTCCATGCTG 
ACTGGAATGT TTCCTCCCAC AAGTGGTACT GCAGTCGTTT GTGGACACGA CATTAAAACG 
GATATGGACG GAGTAAGAGA AAGTCTGGGT CTATGTCCTC AACATAACAT CATCTTCGAC 
GAACTCACCG TCCGAGAGCA TCTCTACTTT TTCAGTAAAC TTAAAGGACT GAAACGTGAA 
GCAATCAATG AAGAAATTGA TAAATACGTC GAACTGTTGG AACTGCAACC TAAAGCGAAT 
GCAAAATCGG CCACTTTGTC AGGAGGAATG AAGAGAAAAC TTTGTGTGGG TATGGCATTG 
TGTGGCAACT CTAAAGTTGT CATGCTTGAT GAACCCACTG CCGGAATGGA TCCATCAGCA 
AGACGAGCTT TGTGGGATTT GCTTCAAAAT CAAAAACACG GAAGGACGAT GCTTTTAACA 
ACACATTTTA TGGACGAAGC TGATTTATTA GGTGATAGAA TCGCTATTAT GGCCGGAGGG 
GAGTTACAGT GTTGCGGCTC AAGTTTCTTC CTAAAGAAGA AATACGGGAC GGGATACCAT 
CTCATAATGG ATAAGGCTTC GAATTGTCAA GAGGAACAAG TAACACAACT TTTGAAGCGA 
CATATTCCTA ATATCCAGGT ACACGGTAAT GTTGGCTCTG AACTCACGTA TCTACTATCC 
GAAGATCACT CTTCAGTTTT TGAAGAAATG CTGCGAGACT TAGAAGAAAA TAGTGATAGT 
TTGGGTATTC GTAGTTACGG AATTTCACTA ACAACTTTAG AAGAAGTTTT CATGAAAGTC 
GGTGCTGACC ACGGCCAGGA AGAAAGCTAT AACGGTTTTG TGCAAGAAAC AGATTGTAAT 
AAAACAATGC TGGGTAAAAT AAAATCGAAA TTGAAAAGGA ATGAAAACTA CGAAAATAGC 
ATTAACGAAA CAACTTTAAA CCTTGAAACT TCTGCTCTTC TAACCGGTTT CATGTTAATC 
AAAAATCAGT TTTTGGCAAT GCTTATGAAA AAAATTTTAT CAATCGTACG AACCTGGATA 
TTGCAGTTTA TTCAAATTTT GATGCCAGTT GCGTTTCTTA TCATAGCCAT TGTCGTTAGC 
AGAAACACTA ACAAATCAGC AGATTTACCC AAACTGCCCA TAACATTAGA TTCGTATCGC 
AATCCAGTAA CGCTAATTGA AGATAATGCA AATAACTCAT ATTCGGCCTT GTATATGCAA 
AGCCTTAAAG GTTATGAAAT TCAAAGAGTG GCAAGCATCC ACGATGTAAT GCTTAATTTG 
ACCGTAACTG TTCCATCCAC TGTCAGACTA AGATACATTG TTGGAGCTAG TTTCAACAAA 
ACATTCGGCG GTGTTTTTAC AACTATCACA GCCTGGTTTA ATAATAACCC TTACCACTCC 
CCGCCTCTAG CATTAGGTCT TGCCTTAAAC GTGGCTTTCA AGCATTTAGC TGGCACAAAT 
CGTTCAATAA GATGGGCAAA TTATCCGTTA CCGTTTACAG CCGATACAAA AGTGCAGCAA 
CTGTTGGAAG GCAACAGTAT GGGTTTCCAG CTCGCTTTCA ACATCGGATT TAGTATGGCT 
TTTGTATCCT CATTCTACGT CCTATTTTAT GTGCGGGAAC GAGTTTGCAA GTCAAAACAT 
TTACAATTCG TGTCCGGAGT GAAAGTTTAC ATATTCTGGA TAATGTCATT CGTCTGTGAT 
ATAATCACGT TCATTGTGAC CATCATCGCG ATCATCATTA CTTTGGCGTG TTTCCAAGAA 
GATGGCTTCC GGTCGGCTGA GGACTTAGGT CGCATGTTCC TTCTTTTGTT CTATTTTGGC 
GTTGCAATGT TGCCTATGAT GTACTTTGCG TCATACTTGT TCGAAGTACC TTCGACAGGT 
TATACTAGAA TGACCCTCTT TAGTGTTTTC ACTGGAGTGG CTGCCTTTTT GGTTGTTCAA 
GTTTTGGCAA CACCTGGATT GGATTTGGAA TATGTCGCGA ATACTCTACA TTGGATTTTC 
CTGGTAGTAC CCCATTATTC GTTAGCTACA GGTATTAGAG ACGTTTATAC AACATTTGCC 
ACTAATAAAA TGTGCAAATA CGCCGTCCAA ACGTGCGTCG AGAATAGGCC AGATATGACT 
GTGGACAAGT GTTGGGATTT GACTTGTCAT AATGCAAGTG CTGTGCTACA GGATTACTGT 
TGTGTACGCG AGCCGCACTA TTACAAATGG GCCAGTCCTG GAATAGGCCG AAATTTGCTA 
TACTCATTTT CCGTTGGAAT AGTCCTATTT ATTCTACTCA ACATAAAAGA ATATAAAATA 
TTTTCAAAAA CGATAGACCA TATTGTTGAG AGCTATAGAA ACAAATTGCC CCAGCCAGTG 
GAAAATGAAG ACAGCGATGT CGCGGAAGAA AAACACAAAA TCCACAACAC TTCTGAAACA 
GAGTTACAAA AAGACTACAT TTTGGTGCTT AAAGATTTGA CAAAGTATTA CAACAACTTT 
TTGGCAGTTA ACGGATTGTG TCTGGGTGTT AAGGAACGTG AATGCTTCGG ATTATTGGGT 
ATAAACGGTG CTGGGAAAAC CACAACTTTT AAAATGATGA CCGGTGATGT AAAAATCACG 
CACGGAGAAG CTTGGGTGAA AAAATTCAGT ATCAAATCGC AACTAAAACA AGTGCAAAAG 
TTGATTGGAT ATTGTCCGCA ATTTGACGCT CTTCTTGATG ACCTGACTGC CAAAGAATCA 
TTGGTAATGT TTGCACTTTT GAGAGGAGTT CCAATGGACG AGTGCCGATA CCTGGTCCAG 
AAACTATCGA CAGATTTTGA TTTCCAAAGA CATTTGAATA AAAAGGTTAA GGAACTTAGT 
GGTGGTAACA AGAGGAAGTT AAGTACTGCC ATTTCGCTGA TTGGCGATCC TCCGGTGATA 
TATTTAGACG AACCTACGAC AGGAATGGAC CCTGCGACGA AACGCTATTT GTGGGATGCC 
ATCTGCAAAG TTAGAGATAA CGGAAAATGT GTAGTCTTGA CCTCTCATAG TATGGAAGAA 
TGTGAAGCTT TGTGCACAAG ACTAGCCATA ATGGTTAATG GCAATTTCAA ATGTTTGGGT 
TCCACTCAAC ATTTGAAAAA TAAATTTGCC GAAGGGTATA CGTTGACAAT TAAAATTAAG 
AAAAGCCCTG GCGGGGAAGA TGCCGATATT AAACCAATTG AAAATTTCAT CTCGGACCAT 
TTTCCCAAAG CAATACAAAG AGAAAAACAT CAAGAGCTTT TAACATATTA TATTGTAGAT 
AAAAGCATCC CCTGGTCCAG AATGTTCGGG ATTTTGGAAA AAGGAAAAAA GCAGCTAAAT 
ATTGAAGATT ATTCTTTGGG TCAATCAAGT TTGGAACAGG TATTTTTATC TTTCACAAGG 
TTGCAACGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015840359.1
CDS	159..5390
Translation	MGKKLDKFLLLMWKNWLLQYRKPLQTLVEILAPVLFSILLVVIRSLVDPETNDPKIYTPFCLLPILCSDESISTNPKFLTNLGNTTFRNFTLVYSPYSNKAVRRSMGILETVLGGHVVGYNNSDQLEAHFLDSNSSLTFAAIQLDDSLAHQDTLPDDLEVSLRFPSELRIGFDLFGQNWHTNLLYPVYQVPGPRAPENNTGALPEKLRDLDRRKRTWRTNLLFPIYQSPGPRSPRSDIGGKPGYFKEGFLTLQLVMTYIFAIGGHLEAQNLTQFQNIITTLLPPLIKMQRFPYGSWIEDPLLQALESFVGVIIMLSFVYTCINTVKVITTEKEKQLKEAMKIMGLPNWLHWTAWFIKTFVFLLVSVVLIILLLKISWYSHTEYSVFTYADPTVMLVFLVLYVCATITFCFAISVFFSKANTAATVAGLVWFLSYAPYLFLQKQYDQLSLSTKLIASLGSNTAMAYGFQLMLMYEGTSEGIQWSNIGRPNTPDDSLTLGYIMMMLVIDTFIYLFVALYVEAVFPGEYGVPLPWYFPFTSAYWCGHPSYVGVEDFTSVPSQEGEFFEKEPNNLTPGIQIRNLRKVFDQKAAVRNLSLNMYNDQITVLLGHNGAGKTTTMSMLTGMFPPTSGTAVVCGHDIKTDMDGVRESLGLCPQHNIIFDELTVREHLYFFSKLKGLKREAINEEIDKYVELLELQPKANAKSATLSGGMKRKLCVGMALCGNSKVVMLDEPTAGMDPSARRALWDLLQNQKHGRTMLLTTHFMDEADLLGDRIAIMAGGELQCCGSSFFLKKKYGTGYHLIMDKASNCQEEQVTQLLKRHIPNIQVHGNVGSELTYLLSEDHSSVFEEMLRDLEENSDSLGIRSYGISLTTLEEVFMKVGADHGQEESYNGFVQETDCNKTMLGKIKSKLKRNENYENSINETTLNLETSALLTGFMLIKNQFLAMLMKKILSIVRTWILQFIQILMPVAFLIIAIVVSRNTNKSADLPKLPITLDSYRNPVTLIEDNANNSYSALYMQSLKGYEIQRVASIHDVMLNLTVTVPSTVRLRYIVGASFNKTFGGVFTTITAWFNNNPYHSPPLALGLALNVAFKHLAGTNRSIRWANYPLPFTADTKVQQLLEGNSMGFQLAFNIGFSMAFVSSFYVLFYVRERVCKSKHLQFVSGVKVYIFWIMSFVCDIITFIVTIIAIIITLACFQEDGFRSAEDLGRMFLLLFYFGVAMLPMMYFASYLFEVPSTGYTRMTLFSVFTGVAAFLVVQVLATPGLDLEYVANTLHWIFLVVPHYSLATGIRDVYTTFATNKMCKYAVQTCVENRPDMTVDKCWDLTCHNASAVLQDYCCVREPHYYKWASPGIGRNLLYSFSVGIVLFILLNIKEYKIFSKTIDHIVESYRNKLPQPVENEDSDVAEEKHKIHNTSETELQKDYILVLKDLTKYYNNFLAVNGLCLGVKERECFGLLGINGAGKTTTFKMMTGDVKITHGEAWVKKFSIKSQLKQVQKLIGYCPQFDALLDDLTAKESLVMFALLRGVPMDECRYLVQKLSTDFDFQRHLNKKVKELSGGNKRKLSTAISLIGDPPVIYLDEPTTGMDPATKRYLWDAICKVRDNGKCVVLTSHSMEECEALCTRLAIMVNGNFKCLGSTQHLKNKFAEGYTLTIKIKKSPGGEDADIKPIENFISDHFPKAIQREKHQELLTYYIVDKSIPWSRMFGILEKGKKQLNIEDYSLGQSSLEQVFLSFTRLQR

Target ORF information:

RefSeq Version	XM_015984873.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015984873.1

ORF Insert Sequence:

ATGGGGAAGA AACTGGACAA GTTCCTTCTA CTGATGTGGA AGAATTGGTT GCTGCAATAC 
CGGAAGCCTC TCCAAACCCT TGTAGAAATT CTAGCTCCAG TCCTGTTCTC CATCCTCCTA 
GTCGTTATTC GAAGTCTGGT TGATCCTGAA ACGAATGATC CAAAGATTTA CACGCCCTTC 
TGTCTACTCC CAATTTTATG CTCGGATGAA AGTATTAGCA CAAATCCGAA ATTTCTAACG 
AACCTCGGAA ATACGACGTT CAGAAACTTT ACACTAGTGT ATTCACCTTA CTCAAACAAA 
GCCGTAAGAC GGTCAATGGG AATTCTTGAA ACTGTTTTGG GTGGCCACGT GGTTGGTTAC 
AACAACTCTG ACCAACTAGA AGCCCATTTC CTCGATTCGA ATTCCTCTCT CACGTTTGCT 
GCCATCCAAC TTGATGACTC TTTAGCGCAC CAAGACACTC TACCAGACGA CTTGGAAGTA 
TCGCTGAGAT TTCCAAGCGA ACTTCGGATT GGGTTCGATT TGTTTGGGCA AAACTGGCAC 
ACAAATCTTT TGTATCCTGT GTATCAAGTA CCAGGCCCCC GAGCGCCAGA GAACAACACA 
GGGGCTCTCC CAGAAAAGTT ACGCGATTTA GATAGAAGAA AAAGAACGTG GAGAACTAAT 
CTTTTATTTC CCATTTATCA ATCACCTGGT CCTAGATCCC CAAGATCAGA CATTGGTGGT 
AAACCAGGTT ACTTCAAAGA AGGATTCTTG ACCTTACAAC TAGTCATGAC GTACATTTTT 
GCAATTGGCG GACACCTTGA AGCGCAAAAT CTAACACAGT TTCAAAATAT TATTACTACA 
CTTCTGCCAC CGCTAATTAA AATGCAACGT TTTCCGTATG GAAGTTGGAT AGAGGATCCT 
TTGCTACAAG CTTTAGAAAG TTTCGTTGGA GTTATTATTA TGTTGAGTTT CGTTTACACT 
TGCATAAACA CGGTTAAAGT TATAACAACC GAAAAAGAGA AACAGCTTAA GGAGGCTATG 
AAAATTATGG GTTTGCCTAA CTGGCTCCAT TGGACTGCGT GGTTTATCAA AACATTTGTC 
TTTCTTTTAG TTTCAGTAGT TTTAATTATT TTACTTTTGA AAATCTCGTG GTATTCACAC 
ACCGAATATA GTGTTTTCAC GTACGCAGAT CCCACAGTAA TGTTGGTGTT CTTAGTACTG 
TACGTTTGTG CAACTATAAC TTTTTGTTTT GCGATTAGCG TATTTTTTTC TAAAGCAAAT 
ACAGCTGCAA CAGTAGCTGG TCTTGTATGG TTCCTCTCTT ATGCTCCATA TTTATTTTTA 
CAAAAGCAGT ATGACCAACT CAGTCTCTCT ACTAAACTCA TAGCAAGTCT TGGGTCAAAC 
ACTGCAATGG CTTACGGGTT CCAGTTAATG TTAATGTATG AGGGTACCAG TGAAGGAATA 
CAATGGTCCA ATATAGGAAG ACCCAATACT CCCGATGACA GTCTAACGCT CGGTTACATA 
ATGATGATGC TGGTAATAGA CACTTTTATC TATCTTTTTG TGGCTCTTTA CGTCGAAGCC 
GTTTTTCCCG GGGAGTACGG AGTGCCACTT CCTTGGTACT TCCCGTTTAC TTCCGCCTAT 
TGGTGTGGTC ATCCTAGCTA TGTAGGAGTT GAAGATTTCA CTAGTGTCCC ATCACAAGAA 
GGTGAATTTT TTGAAAAAGA ACCTAACAAT CTTACACCCG GTATACAAAT TCGAAATTTG 
CGGAAAGTTT TTGACCAAAA AGCAGCAGTT CGAAATCTCT CGCTAAACAT GTACAACGAT 
CAAATTACGG TCCTTCTTGG ACACAATGGT GCCGGAAAAA CTACCACCAT GTCCATGCTG 
ACTGGAATGT TTCCTCCCAC AAGTGGTACT GCAGTCGTTT GTGGACACGA CATTAAAACG 
GATATGGACG GAGTAAGAGA AAGTCTGGGT CTATGTCCTC AACATAACAT CATCTTCGAC 
GAACTCACCG TCCGAGAGCA TCTCTACTTT TTCAGTAAAC TTAAAGGACT GAAACGTGAA 
GCAATCAATG AAGAAATTGA TAAATACGTC GAACTGTTGG AACTGCAACC TAAAGCGAAT 
GCAAAATCGG CCACTTTGTC AGGAGGAATG AAGAGAAAAC TTTGTGTGGG TATGGCATTG 
TGTGGCAACT CTAAAGTTGT CATGCTTGAT GAACCCACTG CCGGAATGGA TCCATCAGCA 
AGACGAGCTT TGTGGGATTT GCTTCAAAAT CAAAAACACG GAAGGACGAT GCTTTTAACA 
ACACATTTTA TGGACGAAGC TGATTTATTA GGTGATAGAA TCGCTATTAT GGCCGGAGGG 
GAGTTACAGT GTTGCGGCTC AAGTTTCTTC CTAAAGAAGA AATACGGGAC GGGATACCAT 
CTCATAATGG ATAAGGCTTC GAATTGTCAA GAGGAACAAG TAACACAACT TTTGAAGCGA 
CATATTCCTA ATATCCAGGT ACACGGTAAT GTTGGCTCTG AACTCACGTA TCTACTATCC 
GAAGATCACT CTTCAGTTTT TGAAGAAATG CTGCGAGACT TAGAAGAAAA TAGTGATAGT 
TTGGGTATTC GTAGTTACGG AATTTCACTA ACAACTTTAG AAGAAGTTTT CATGAAAGTC 
GGTGCTGACC ACGGCCAGGA AGAAAGCTAT AACGGTTTTG TGCAAGAAAC AGATTGTAAT 
AAAACAATGC TGGGTAAAAT AAAATCGAAA TTGAAAAGGA ATGAAAACTA CGAAAATAGC 
ATTAACGAAA CAACTTTAAA CCTTGAAACT TCTGCTCTTC TAACCGGTTT CATGTTAATC 
AAAAATCAGT TTTTGGCAAT GCTTATGAAA AAAATTTTAT CAATCGTACG AACCTGGATA 
TTGCAGTTTA TTCAAATTTT GATGCCAGTT GCGTTTCTTA TCATAGCCAT TGTCGTTAGC 
AGAAACACTA ACAAATCAGC AGATTTACCC AAACTGCCCA TAACATTAGA TTCGTATCGC 
AATCCAGTAA CGCTAATTGA AGATAATGCA AATAACTCAT ATTCGGCCTT GTATATGCAA 
AGCCTTAAAG GTTATGAAAT TCAAAGAGTG GCAAGCATCC ACGATGTAAT GCTTAATTTG 
ACCGTAACTG TTCCATCCAC TGTCAGACTA AGATACATTG TTGGAGCTAG TTTCAACAAA 
ACATTCGGCG GTGTTTTTAC AACTATCACA GCCTGGTTTA ATAATAACCC TTACCACTCC 
CCGCCTCTAG CATTAGGTCT TGCCTTAAAC GTGGCTTTCA AGCATTTAGC TGGCACAAAT 
CGTTCAATAA GATGGGCAAA TTATCCGTTA CCGTTTACAG CCGATACAAA AGTGCAGCAA 
CTGTTGGAAG GCAACAGTAT GGGTTTCCAG CTCGCTTTCA ACATCGGATT TAGTATGGCT 
TTTGTATCCT CATTCTACGT CCTATTTTAT GTGCGGGAAC GAGTTTGCAA GTCAAAACAT 
TTACAATTCG TGTCCGGAGT GAAAGTTTAC ATATTCTGGA TAATGTCATT CGTCTGTGAT 
ATAATCACGT TCATTGTGAC CATCATCGCG ATCATCATTA CTTTGGCGTG TTTCCAAGAA 
GATGGCTTCC GGTCGGCTGA GGACTTAGGT CGCATGTTCC TTCTTTTGTT CTATTTTGGC 
GTTGCAATGT TGCCTATGAT GTACTTTGCG TCATACTTGT TCGAAGTACC TTCGACAGGT 
TATACTAGAA TGACCCTCTT TAGTGTTTTC ACTGGAGTGG CTGCCTTTTT GGTTGTTCAA 
GTTTTGGCAA CACCTGGATT GGATTTGGAA TATGTCGCGA ATACTCTACA TTGGATTTTC 
CTGGTAGTAC CCCATTATTC GTTAGCTACA GGTATTAGAG ACGTTTATAC AACATTTGCC 
ACTAATAAAA TGTGCAAATA CGCCGTCCAA ACGTGCGTCG AGAATAGGCC AGATATGACT 
GTGGACAAGT GTTGGGATTT GACTTGTCAT AATGCAAGTG CTGTGCTACA GGATTACTGT 
TGTGTACGCG AGCCGCACTA TTACAAATGG GCCAGTCCTG GAATAGGCCG AAATTTGCTA 
TACTCATTTT CCGTTGGAAT AGTCCTATTT ATTCTACTCA ACATAAAAGA ATATAAAATA 
TTTTCAAAAA CGATAGACCA TATTGTTGAG AGCTATAGAA ACAAATTGCC CCAGCCAGTG 
GAAAATGAAG ACAGCGATGT CGCGGAAGAA AAACACAAAA TCCACAACAC TTCTGAAACA 
GAGTTACAAA AAGACTACAT TTTGGTGCTT AAAGATTTGA CAAAGTATTA CAACAACTTT 
TTGGCAGTTA ACGGATTGTG TCTGGGTGTT AAGGAACGTG AATGCTTCGG ATTATTGGGT 
ATAAACGGTG CTGGGAAAAC CACAACTTTT AAAATGATGA CCGGTGATGT AAAAATCACG 
CACGGAGAAG CTTGGGTGAA AAAATTCAGT ATCAAATCGC AACTAAAACA AGTGCAAAAG 
TTGATTGGAT ATTGTCCGCA ATTTGACGCT CTTCTTGATG ACCTGACTGC CAAAGAATCA 
TTGGTAATGT TTGCACTTTT GAGAGGAGTT CCAATGGACG AGTGCCGATA CCTGGTCCAG 
AAACTATCGA CAGATTTTGA TTTCCAAAGA CATTTGAATA AAAAGGTTAA GGAACTTAGT 
GGTGGTAACA AGAGGAAGTT AAGTACTGCC ATTTCGCTGA TTGGCGATCC TCCGGTGATA 
TATTTAGACG AACCTACGAC AGGAATGGAC CCTGCGACGA AACGCTATTT GTGGGATGCC 
ATCTGCAAAG TTAGAGATAA CGGAAAATGT GTAGTCTTGA CCTCTCATAG TATGGAAGAA 
TGTGAAGCTT TGTGCACAAG ACTAGCCATA ATGGTTAATG GCAATTTCAA ATGTTTGGGT 
TCCACTCAAC ATTTGAAAAA TAAATTTGCC GAAGGGTATA CGTTGACAAT TAAAATTAAG 
AAAAGCCCTG GCGGGGAAGA TGCCGATATT AAACCAATTG AAAATTTCAT CTCGGACCAT 
TTTCCCAAAG CAATACAAAG AGAAAAACAT CAAGAGCTTT TAACATATTA TATTGTAGAT 
AAAAGCATCC CCTGGTCCAG AATGTTCGGG ATTTTGGAAA AAGGAAAAAA GCAGCTAAAT 
ATTGAAGATT ATTCTTTGGG TCAATCAAGT TTGGAACAGG TATTTTTATC TTTCACAAGG 
TTGCAACGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC659344 ( XM_008200929.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008199151.1 Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X4, mRNA.

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CloneID	OTg14533
Clone ID Related Accession (Same CDS sequence)	XM_015984873.1 , XM_008200929.2 , XM_015984872.1 , XM_008200930.2
Accession Version	XM_008200929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	ATP-binding cassette sub-family A member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015451937.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008200929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AACTGGACAA GTTCCTTCTA CTGATGTGGA AGAATTGGTT GCTGCAATAC 
CGGAAGCCTC TCCAAACCCT TGTAGAAATT CTAGCTCCAG TCCTGTTCTC CATCCTCCTA 
GTCGTTATTC GAAGTCTGGT TGATCCTGAA ACGAATGATC CAAAGATTTA CACGCCCTTC 
TGTCTACTCC CAATTTTATG CTCGGATGAA AGTATTAGCA CAAATCCGAA ATTTCTAACG 
AACCTCGGAA ATACGACGTT CAGAAACTTT ACACTAGTGT ATTCACCTTA CTCAAACAAA 
GCCGTAAGAC GGTCAATGGG AATTCTTGAA ACTGTTTTGG GTGGCCACGT GGTTGGTTAC 
AACAACTCTG ACCAACTAGA AGCCCATTTC CTCGATTCGA ATTCCTCTCT CACGTTTGCT 
GCCATCCAAC TTGATGACTC TTTAGCGCAC CAAGACACTC TACCAGACGA CTTGGAAGTA 
TCGCTGAGAT TTCCAAGCGA ACTTCGGATT GGGTTCGATT TGTTTGGGCA AAACTGGCAC 
ACAAATCTTT TGTATCCTGT GTATCAAGTA CCAGGCCCCC GAGCGCCAGA GAACAACACA 
GGGGCTCTCC CAGAAAAGTT ACGCGATTTA GATAGAAGAA AAAGAACGTG GAGAACTAAT 
CTTTTATTTC CCATTTATCA ATCACCTGGT CCTAGATCCC CAAGATCAGA CATTGGTGGT 
AAACCAGGTT ACTTCAAAGA AGGATTCTTG ACCTTACAAC TAGTCATGAC GTACATTTTT 
GCAATTGGCG GACACCTTGA AGCGCAAAAT CTAACACAGT TTCAAAATAT TATTACTACA 
CTTCTGCCAC CGCTAATTAA AATGCAACGT TTTCCGTATG GAAGTTGGAT AGAGGATCCT 
TTGCTACAAG CTTTAGAAAG TTTCGTTGGA GTTATTATTA TGTTGAGTTT CGTTTACACT 
TGCATAAACA CGGTTAAAGT TATAACAACC GAAAAAGAGA AACAGCTTAA GGAGGCTATG 
AAAATTATGG GTTTGCCTAA CTGGCTCCAT TGGACTGCGT GGTTTATCAA AACATTTGTC 
TTTCTTTTAG TTTCAGTAGT TTTAATTATT TTACTTTTGA AAATCTCGTG GTATTCACAC 
ACCGAATATA GTGTTTTCAC GTACGCAGAT CCCACAGTAA TGTTGGTGTT CTTAGTACTG 
TACGTTTGTG CAACTATAAC TTTTTGTTTT GCGATTAGCG TATTTTTTTC TAAAGCAAAT 
ACAGCTGCAA CAGTAGCTGG TCTTGTATGG TTCCTCTCTT ATGCTCCATA TTTATTTTTA 
CAAAAGCAGT ATGACCAACT CAGTCTCTCT ACTAAACTCA TAGCAAGTCT TGGGTCAAAC 
ACTGCAATGG CTTACGGGTT CCAGTTAATG TTAATGTATG AGGGTACCAG TGAAGGAATA 
CAATGGTCCA ATATAGGAAG ACCCAATACT CCCGATGACA GTCTAACGCT CGGTTACATA 
ATGATGATGC TGGTAATAGA CACTTTTATC TATCTTTTTG TGGCTCTTTA CGTCGAAGCC 
GTTTTTCCCG GGGAGTACGG AGTGCCACTT CCTTGGTACT TCCCGTTTAC TTCCGCCTAT 
TGGTGTGGTC ATCCTAGCTA TGTAGGAGTT GAAGATTTCA CTAGTGTCCC ATCACAAGAA 
GGTGAATTTT TTGAAAAAGA ACCTAACAAT CTTACACCCG GTATACAAAT TCGAAATTTG 
CGGAAAGTTT TTGACCAAAA AGCAGCAGTT CGAAATCTCT CGCTAAACAT GTACAACGAT 
CAAATTACGG TCCTTCTTGG ACACAATGGT GCCGGAAAAA CTACCACCAT GTCCATGCTG 
ACTGGAATGT TTCCTCCCAC AAGTGGTACT GCAGTCGTTT GTGGACACGA CATTAAAACG 
GATATGGACG GAGTAAGAGA AAGTCTGGGT CTATGTCCTC AACATAACAT CATCTTCGAC 
GAACTCACCG TCCGAGAGCA TCTCTACTTT TTCAGTAAAC TTAAAGGACT GAAACGTGAA 
GCAATCAATG AAGAAATTGA TAAATACGTC GAACTGTTGG AACTGCAACC TAAAGCGAAT 
GCAAAATCGG CCACTTTGTC AGGAGGAATG AAGAGAAAAC TTTGTGTGGG TATGGCATTG 
TGTGGCAACT CTAAAGTTGT CATGCTTGAT GAACCCACTG CCGGAATGGA TCCATCAGCA 
AGACGAGCTT TGTGGGATTT GCTTCAAAAT CAAAAACACG GAAGGACGAT GCTTTTAACA 
ACACATTTTA TGGACGAAGC TGATTTATTA GGTGATAGAA TCGCTATTAT GGCCGGAGGG 
GAGTTACAGT GTTGCGGCTC AAGTTTCTTC CTAAAGAAGA AATACGGGAC GGGATACCAT 
CTCATAATGG ATAAGGCTTC GAATTGTCAA GAGGAACAAG TAACACAACT TTTGAAGCGA 
CATATTCCTA ATATCCAGGT ACACGGTAAT GTTGGCTCTG AACTCACGTA TCTACTATCC 
GAAGATCACT CTTCAGTTTT TGAAGAAATG CTGCGAGACT TAGAAGAAAA TAGTGATAGT 
TTGGGTATTC GTAGTTACGG AATTTCACTA ACAACTTTAG AAGAAGTTTT CATGAAAGTC 
GGTGCTGACC ACGGCCAGGA AGAAAGCTAT AACGGTTTTG TGCAAGAAAC AGATTGTAAT 
AAAACAATGC TGGGTAAAAT AAAATCGAAA TTGAAAAGGA ATGAAAACTA CGAAAATAGC 
ATTAACGAAA CAACTTTAAA CCTTGAAACT TCTGCTCTTC TAACCGGTTT CATGTTAATC 
AAAAATCAGT TTTTGGCAAT GCTTATGAAA AAAATTTTAT CAATCGTACG AACCTGGATA 
TTGCAGTTTA TTCAAATTTT GATGCCAGTT GCGTTTCTTA TCATAGCCAT TGTCGTTAGC 
AGAAACACTA ACAAATCAGC AGATTTACCC AAACTGCCCA TAACATTAGA TTCGTATCGC 
AATCCAGTAA CGCTAATTGA AGATAATGCA AATAACTCAT ATTCGGCCTT GTATATGCAA 
AGCCTTAAAG GTTATGAAAT TCAAAGAGTG GCAAGCATCC ACGATGTAAT GCTTAATTTG 
ACCGTAACTG TTCCATCCAC TGTCAGACTA AGATACATTG TTGGAGCTAG TTTCAACAAA 
ACATTCGGCG GTGTTTTTAC AACTATCACA GCCTGGTTTA ATAATAACCC TTACCACTCC 
CCGCCTCTAG CATTAGGTCT TGCCTTAAAC GTGGCTTTCA AGCATTTAGC TGGCACAAAT 
CGTTCAATAA GATGGGCAAA TTATCCGTTA CCGTTTACAG CCGATACAAA AGTGCAGCAA 
CTGTTGGAAG GCAACAGTAT GGGTTTCCAG CTCGCTTTCA ACATCGGATT TAGTATGGCT 
TTTGTATCCT CATTCTACGT CCTATTTTAT GTGCGGGAAC GAGTTTGCAA GTCAAAACAT 
TTACAATTCG TGTCCGGAGT GAAAGTTTAC ATATTCTGGA TAATGTCATT CGTCTGTGAT 
ATAATCACGT TCATTGTGAC CATCATCGCG ATCATCATTA CTTTGGCGTG TTTCCAAGAA 
GATGGCTTCC GGTCGGCTGA GGACTTAGGT CGCATGTTCC TTCTTTTGTT CTATTTTGGC 
GTTGCAATGT TGCCTATGAT GTACTTTGCG TCATACTTGT TCGAAGTACC TTCGACAGGT 
TATACTAGAA TGACCCTCTT TAGTGTTTTC ACTGGAGTGG CTGCCTTTTT GGTTGTTCAA 
GTTTTGGCAA CACCTGGATT GGATTTGGAA TATGTCGCGA ATACTCTACA TTGGATTTTC 
CTGGTAGTAC CCCATTATTC GTTAGCTACA GGTATTAGAG ACGTTTATAC AACATTTGCC 
ACTAATAAAA TGTGCAAATA CGCCGTCCAA ACGTGCGTCG AGAATAGGCC AGATATGACT 
GTGGACAAGT GTTGGGATTT GACTTGTCAT AATGCAAGTG CTGTGCTACA GGATTACTGT 
TGTGTACGCG AGCCGCACTA TTACAAATGG GCCAGTCCTG GAATAGGCCG AAATTTGCTA 
TACTCATTTT CCGTTGGAAT AGTCCTATTT ATTCTACTCA ACATAAAAGA ATATAAAATA 
TTTTCAAAAA CGATAGACCA TATTGTTGAG AGCTATAGAA ACAAATTGCC CCAGCCAGTG 
GAAAATGAAG ACAGCGATGT CGCGGAAGAA AAACACAAAA TCCACAACAC TTCTGAAACA 
GAGTTACAAA AAGACTACAT TTTGGTGCTT AAAGATTTGA CAAAGTATTA CAACAACTTT 
TTGGCAGTTA ACGGATTGTG TCTGGGTGTT AAGGAACGTG AATGCTTCGG ATTATTGGGT 
ATAAACGGTG CTGGGAAAAC CACAACTTTT AAAATGATGA CCGGTGATGT AAAAATCACG 
CACGGAGAAG CTTGGGTGAA AAAATTCAGT ATCAAATCGC AACTAAAACA AGTGCAAAAG 
TTGATTGGAT ATTGTCCGCA ATTTGACGCT CTTCTTGATG ACCTGACTGC CAAAGAATCA 
TTGGTAATGT TTGCACTTTT GAGAGGAGTT CCAATGGACG AGTGCCGATA CCTGGTCCAG 
AAACTATCGA CAGATTTTGA TTTCCAAAGA CATTTGAATA AAAAGGTTAA GGAACTTAGT 
GGTGGTAACA AGAGGAAGTT AAGTACTGCC ATTTCGCTGA TTGGCGATCC TCCGGTGATA 
TATTTAGACG AACCTACGAC AGGAATGGAC CCTGCGACGA AACGCTATTT GTGGGATGCC 
ATCTGCAAAG TTAGAGATAA CGGAAAATGT GTAGTCTTGA CCTCTCATAG TATGGAAGAA 
TGTGAAGCTT TGTGCACAAG ACTAGCCATA ATGGTTAATG GCAATTTCAA ATGTTTGGGT 
TCCACTCAAC ATTTGAAAAA TAAATTTGCC GAAGGGTATA CGTTGACAAT TAAAATTAAG 
AAAAGCCCTG GCGGGGAAGA TGCCGATATT AAACCAATTG AAAATTTCAT CTCGGACCAT 
TTTCCCAAAG CAATACAAAG AGAAAAACAT CAAGAGCTTT TAACATATTA TATTGTAGAT 
AAAAGCATCC CCTGGTCCAG AATGTTCGGG ATTTTGGAAA AAGGAAAAAA GCAGCTAAAT 
ATTGAAGATT ATTCTTTGGG TCAATCAAGT TTGGAACAGG TATTTTTATC TTTCACAAGG 
TTGCAACGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008199151.1
CDS	175..5406
Translation	MGKKLDKFLLLMWKNWLLQYRKPLQTLVEILAPVLFSILLVVIRSLVDPETNDPKIYTPFCLLPILCSDESISTNPKFLTNLGNTTFRNFTLVYSPYSNKAVRRSMGILETVLGGHVVGYNNSDQLEAHFLDSNSSLTFAAIQLDDSLAHQDTLPDDLEVSLRFPSELRIGFDLFGQNWHTNLLYPVYQVPGPRAPENNTGALPEKLRDLDRRKRTWRTNLLFPIYQSPGPRSPRSDIGGKPGYFKEGFLTLQLVMTYIFAIGGHLEAQNLTQFQNIITTLLPPLIKMQRFPYGSWIEDPLLQALESFVGVIIMLSFVYTCINTVKVITTEKEKQLKEAMKIMGLPNWLHWTAWFIKTFVFLLVSVVLIILLLKISWYSHTEYSVFTYADPTVMLVFLVLYVCATITFCFAISVFFSKANTAATVAGLVWFLSYAPYLFLQKQYDQLSLSTKLIASLGSNTAMAYGFQLMLMYEGTSEGIQWSNIGRPNTPDDSLTLGYIMMMLVIDTFIYLFVALYVEAVFPGEYGVPLPWYFPFTSAYWCGHPSYVGVEDFTSVPSQEGEFFEKEPNNLTPGIQIRNLRKVFDQKAAVRNLSLNMYNDQITVLLGHNGAGKTTTMSMLTGMFPPTSGTAVVCGHDIKTDMDGVRESLGLCPQHNIIFDELTVREHLYFFSKLKGLKREAINEEIDKYVELLELQPKANAKSATLSGGMKRKLCVGMALCGNSKVVMLDEPTAGMDPSARRALWDLLQNQKHGRTMLLTTHFMDEADLLGDRIAIMAGGELQCCGSSFFLKKKYGTGYHLIMDKASNCQEEQVTQLLKRHIPNIQVHGNVGSELTYLLSEDHSSVFEEMLRDLEENSDSLGIRSYGISLTTLEEVFMKVGADHGQEESYNGFVQETDCNKTMLGKIKSKLKRNENYENSINETTLNLETSALLTGFMLIKNQFLAMLMKKILSIVRTWILQFIQILMPVAFLIIAIVVSRNTNKSADLPKLPITLDSYRNPVTLIEDNANNSYSALYMQSLKGYEIQRVASIHDVMLNLTVTVPSTVRLRYIVGASFNKTFGGVFTTITAWFNNNPYHSPPLALGLALNVAFKHLAGTNRSIRWANYPLPFTADTKVQQLLEGNSMGFQLAFNIGFSMAFVSSFYVLFYVRERVCKSKHLQFVSGVKVYIFWIMSFVCDIITFIVTIIAIIITLACFQEDGFRSAEDLGRMFLLLFYFGVAMLPMMYFASYLFEVPSTGYTRMTLFSVFTGVAAFLVVQVLATPGLDLEYVANTLHWIFLVVPHYSLATGIRDVYTTFATNKMCKYAVQTCVENRPDMTVDKCWDLTCHNASAVLQDYCCVREPHYYKWASPGIGRNLLYSFSVGIVLFILLNIKEYKIFSKTIDHIVESYRNKLPQPVENEDSDVAEEKHKIHNTSETELQKDYILVLKDLTKYYNNFLAVNGLCLGVKERECFGLLGINGAGKTTTFKMMTGDVKITHGEAWVKKFSIKSQLKQVQKLIGYCPQFDALLDDLTAKESLVMFALLRGVPMDECRYLVQKLSTDFDFQRHLNKKVKELSGGNKRKLSTAISLIGDPPVIYLDEPTTGMDPATKRYLWDAICKVRDNGKCVVLTSHSMEECEALCTRLAIMVNGNFKCLGSTQHLKNKFAEGYTLTIKIKKSPGGEDADIKPIENFISDHFPKAIQREKHQELLTYYIVDKSIPWSRMFGILEKGKKQLNIEDYSLGQSSLEQVFLSFTRLQR

Target ORF information:

RefSeq Version	XM_008200929.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008200929.2

ORF Insert Sequence:

ATGGGGAAGA AACTGGACAA GTTCCTTCTA CTGATGTGGA AGAATTGGTT GCTGCAATAC 
CGGAAGCCTC TCCAAACCCT TGTAGAAATT CTAGCTCCAG TCCTGTTCTC CATCCTCCTA 
GTCGTTATTC GAAGTCTGGT TGATCCTGAA ACGAATGATC CAAAGATTTA CACGCCCTTC 
TGTCTACTCC CAATTTTATG CTCGGATGAA AGTATTAGCA CAAATCCGAA ATTTCTAACG 
AACCTCGGAA ATACGACGTT CAGAAACTTT ACACTAGTGT ATTCACCTTA CTCAAACAAA 
GCCGTAAGAC GGTCAATGGG AATTCTTGAA ACTGTTTTGG GTGGCCACGT GGTTGGTTAC 
AACAACTCTG ACCAACTAGA AGCCCATTTC CTCGATTCGA ATTCCTCTCT CACGTTTGCT 
GCCATCCAAC TTGATGACTC TTTAGCGCAC CAAGACACTC TACCAGACGA CTTGGAAGTA 
TCGCTGAGAT TTCCAAGCGA ACTTCGGATT GGGTTCGATT TGTTTGGGCA AAACTGGCAC 
ACAAATCTTT TGTATCCTGT GTATCAAGTA CCAGGCCCCC GAGCGCCAGA GAACAACACA 
GGGGCTCTCC CAGAAAAGTT ACGCGATTTA GATAGAAGAA AAAGAACGTG GAGAACTAAT 
CTTTTATTTC CCATTTATCA ATCACCTGGT CCTAGATCCC CAAGATCAGA CATTGGTGGT 
AAACCAGGTT ACTTCAAAGA AGGATTCTTG ACCTTACAAC TAGTCATGAC GTACATTTTT 
GCAATTGGCG GACACCTTGA AGCGCAAAAT CTAACACAGT TTCAAAATAT TATTACTACA 
CTTCTGCCAC CGCTAATTAA AATGCAACGT TTTCCGTATG GAAGTTGGAT AGAGGATCCT 
TTGCTACAAG CTTTAGAAAG TTTCGTTGGA GTTATTATTA TGTTGAGTTT CGTTTACACT 
TGCATAAACA CGGTTAAAGT TATAACAACC GAAAAAGAGA AACAGCTTAA GGAGGCTATG 
AAAATTATGG GTTTGCCTAA CTGGCTCCAT TGGACTGCGT GGTTTATCAA AACATTTGTC 
TTTCTTTTAG TTTCAGTAGT TTTAATTATT TTACTTTTGA AAATCTCGTG GTATTCACAC 
ACCGAATATA GTGTTTTCAC GTACGCAGAT CCCACAGTAA TGTTGGTGTT CTTAGTACTG 
TACGTTTGTG CAACTATAAC TTTTTGTTTT GCGATTAGCG TATTTTTTTC TAAAGCAAAT 
ACAGCTGCAA CAGTAGCTGG TCTTGTATGG TTCCTCTCTT ATGCTCCATA TTTATTTTTA 
CAAAAGCAGT ATGACCAACT CAGTCTCTCT ACTAAACTCA TAGCAAGTCT TGGGTCAAAC 
ACTGCAATGG CTTACGGGTT CCAGTTAATG TTAATGTATG AGGGTACCAG TGAAGGAATA 
CAATGGTCCA ATATAGGAAG ACCCAATACT CCCGATGACA GTCTAACGCT CGGTTACATA 
ATGATGATGC TGGTAATAGA CACTTTTATC TATCTTTTTG TGGCTCTTTA CGTCGAAGCC 
GTTTTTCCCG GGGAGTACGG AGTGCCACTT CCTTGGTACT TCCCGTTTAC TTCCGCCTAT 
TGGTGTGGTC ATCCTAGCTA TGTAGGAGTT GAAGATTTCA CTAGTGTCCC ATCACAAGAA 
GGTGAATTTT TTGAAAAAGA ACCTAACAAT CTTACACCCG GTATACAAAT TCGAAATTTG 
CGGAAAGTTT TTGACCAAAA AGCAGCAGTT CGAAATCTCT CGCTAAACAT GTACAACGAT 
CAAATTACGG TCCTTCTTGG ACACAATGGT GCCGGAAAAA CTACCACCAT GTCCATGCTG 
ACTGGAATGT TTCCTCCCAC AAGTGGTACT GCAGTCGTTT GTGGACACGA CATTAAAACG 
GATATGGACG GAGTAAGAGA AAGTCTGGGT CTATGTCCTC AACATAACAT CATCTTCGAC 
GAACTCACCG TCCGAGAGCA TCTCTACTTT TTCAGTAAAC TTAAAGGACT GAAACGTGAA 
GCAATCAATG AAGAAATTGA TAAATACGTC GAACTGTTGG AACTGCAACC TAAAGCGAAT 
GCAAAATCGG CCACTTTGTC AGGAGGAATG AAGAGAAAAC TTTGTGTGGG TATGGCATTG 
TGTGGCAACT CTAAAGTTGT CATGCTTGAT GAACCCACTG CCGGAATGGA TCCATCAGCA 
AGACGAGCTT TGTGGGATTT GCTTCAAAAT CAAAAACACG GAAGGACGAT GCTTTTAACA 
ACACATTTTA TGGACGAAGC TGATTTATTA GGTGATAGAA TCGCTATTAT GGCCGGAGGG 
GAGTTACAGT GTTGCGGCTC AAGTTTCTTC CTAAAGAAGA AATACGGGAC GGGATACCAT 
CTCATAATGG ATAAGGCTTC GAATTGTCAA GAGGAACAAG TAACACAACT TTTGAAGCGA 
CATATTCCTA ATATCCAGGT ACACGGTAAT GTTGGCTCTG AACTCACGTA TCTACTATCC 
GAAGATCACT CTTCAGTTTT TGAAGAAATG CTGCGAGACT TAGAAGAAAA TAGTGATAGT 
TTGGGTATTC GTAGTTACGG AATTTCACTA ACAACTTTAG AAGAAGTTTT CATGAAAGTC 
GGTGCTGACC ACGGCCAGGA AGAAAGCTAT AACGGTTTTG TGCAAGAAAC AGATTGTAAT 
AAAACAATGC TGGGTAAAAT AAAATCGAAA TTGAAAAGGA ATGAAAACTA CGAAAATAGC 
ATTAACGAAA CAACTTTAAA CCTTGAAACT TCTGCTCTTC TAACCGGTTT CATGTTAATC 
AAAAATCAGT TTTTGGCAAT GCTTATGAAA AAAATTTTAT CAATCGTACG AACCTGGATA 
TTGCAGTTTA TTCAAATTTT GATGCCAGTT GCGTTTCTTA TCATAGCCAT TGTCGTTAGC 
AGAAACACTA ACAAATCAGC AGATTTACCC AAACTGCCCA TAACATTAGA TTCGTATCGC 
AATCCAGTAA CGCTAATTGA AGATAATGCA AATAACTCAT ATTCGGCCTT GTATATGCAA 
AGCCTTAAAG GTTATGAAAT TCAAAGAGTG GCAAGCATCC ACGATGTAAT GCTTAATTTG 
ACCGTAACTG TTCCATCCAC TGTCAGACTA AGATACATTG TTGGAGCTAG TTTCAACAAA 
ACATTCGGCG GTGTTTTTAC AACTATCACA GCCTGGTTTA ATAATAACCC TTACCACTCC 
CCGCCTCTAG CATTAGGTCT TGCCTTAAAC GTGGCTTTCA AGCATTTAGC TGGCACAAAT 
CGTTCAATAA GATGGGCAAA TTATCCGTTA CCGTTTACAG CCGATACAAA AGTGCAGCAA 
CTGTTGGAAG GCAACAGTAT GGGTTTCCAG CTCGCTTTCA ACATCGGATT TAGTATGGCT 
TTTGTATCCT CATTCTACGT CCTATTTTAT GTGCGGGAAC GAGTTTGCAA GTCAAAACAT 
TTACAATTCG TGTCCGGAGT GAAAGTTTAC ATATTCTGGA TAATGTCATT CGTCTGTGAT 
ATAATCACGT TCATTGTGAC CATCATCGCG ATCATCATTA CTTTGGCGTG TTTCCAAGAA 
GATGGCTTCC GGTCGGCTGA GGACTTAGGT CGCATGTTCC TTCTTTTGTT CTATTTTGGC 
GTTGCAATGT TGCCTATGAT GTACTTTGCG TCATACTTGT TCGAAGTACC TTCGACAGGT 
TATACTAGAA TGACCCTCTT TAGTGTTTTC ACTGGAGTGG CTGCCTTTTT GGTTGTTCAA 
GTTTTGGCAA CACCTGGATT GGATTTGGAA TATGTCGCGA ATACTCTACA TTGGATTTTC 
CTGGTAGTAC CCCATTATTC GTTAGCTACA GGTATTAGAG ACGTTTATAC AACATTTGCC 
ACTAATAAAA TGTGCAAATA CGCCGTCCAA ACGTGCGTCG AGAATAGGCC AGATATGACT 
GTGGACAAGT GTTGGGATTT GACTTGTCAT AATGCAAGTG CTGTGCTACA GGATTACTGT 
TGTGTACGCG AGCCGCACTA TTACAAATGG GCCAGTCCTG GAATAGGCCG AAATTTGCTA 
TACTCATTTT CCGTTGGAAT AGTCCTATTT ATTCTACTCA ACATAAAAGA ATATAAAATA 
TTTTCAAAAA CGATAGACCA TATTGTTGAG AGCTATAGAA ACAAATTGCC CCAGCCAGTG 
GAAAATGAAG ACAGCGATGT CGCGGAAGAA AAACACAAAA TCCACAACAC TTCTGAAACA 
GAGTTACAAA AAGACTACAT TTTGGTGCTT AAAGATTTGA CAAAGTATTA CAACAACTTT 
TTGGCAGTTA ACGGATTGTG TCTGGGTGTT AAGGAACGTG AATGCTTCGG ATTATTGGGT 
ATAAACGGTG CTGGGAAAAC CACAACTTTT AAAATGATGA CCGGTGATGT AAAAATCACG 
CACGGAGAAG CTTGGGTGAA AAAATTCAGT ATCAAATCGC AACTAAAACA AGTGCAAAAG 
TTGATTGGAT ATTGTCCGCA ATTTGACGCT CTTCTTGATG ACCTGACTGC CAAAGAATCA 
TTGGTAATGT TTGCACTTTT GAGAGGAGTT CCAATGGACG AGTGCCGATA CCTGGTCCAG 
AAACTATCGA CAGATTTTGA TTTCCAAAGA CATTTGAATA AAAAGGTTAA GGAACTTAGT 
GGTGGTAACA AGAGGAAGTT AAGTACTGCC ATTTCGCTGA TTGGCGATCC TCCGGTGATA 
TATTTAGACG AACCTACGAC AGGAATGGAC CCTGCGACGA AACGCTATTT GTGGGATGCC 
ATCTGCAAAG TTAGAGATAA CGGAAAATGT GTAGTCTTGA CCTCTCATAG TATGGAAGAA 
TGTGAAGCTT TGTGCACAAG ACTAGCCATA ATGGTTAATG GCAATTTCAA ATGTTTGGGT 
TCCACTCAAC ATTTGAAAAA TAAATTTGCC GAAGGGTATA CGTTGACAAT TAAAATTAAG 
AAAAGCCCTG GCGGGGAAGA TGCCGATATT AAACCAATTG AAAATTTCAT CTCGGACCAT 
TTTCCCAAAG CAATACAAAG AGAAAAACAT CAAGAGCTTT TAACATATTA TATTGTAGAT 
AAAAGCATCC CCTGGTCCAG AATGTTCGGG ATTTTGGAAA AAGGAAAAAA GCAGCTAAAT 
ATTGAAGATT ATTCTTTGGG TCAATCAAGT TTGGAACAGG TATTTTTATC TTTCACAAGG 
TTGCAACGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC659344 ( XM_015984872.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015840358.1 Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X5, mRNA.

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CloneID	OTg14533
Clone ID Related Accession (Same CDS sequence)	XM_015984873.1 , XM_008200929.2 , XM_015984872.1 , XM_008200930.2
Accession Version	XM_015984872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	ATP-binding cassette sub-family A member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015451937.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AACTGGACAA GTTCCTTCTA CTGATGTGGA AGAATTGGTT GCTGCAATAC 
CGGAAGCCTC TCCAAACCCT TGTAGAAATT CTAGCTCCAG TCCTGTTCTC CATCCTCCTA 
GTCGTTATTC GAAGTCTGGT TGATCCTGAA ACGAATGATC CAAAGATTTA CACGCCCTTC 
TGTCTACTCC CAATTTTATG CTCGGATGAA AGTATTAGCA CAAATCCGAA ATTTCTAACG 
AACCTCGGAA ATACGACGTT CAGAAACTTT ACACTAGTGT ATTCACCTTA CTCAAACAAA 
GCCGTAAGAC GGTCAATGGG AATTCTTGAA ACTGTTTTGG GTGGCCACGT GGTTGGTTAC 
AACAACTCTG ACCAACTAGA AGCCCATTTC CTCGATTCGA ATTCCTCTCT CACGTTTGCT 
GCCATCCAAC TTGATGACTC TTTAGCGCAC CAAGACACTC TACCAGACGA CTTGGAAGTA 
TCGCTGAGAT TTCCAAGCGA ACTTCGGATT GGGTTCGATT TGTTTGGGCA AAACTGGCAC 
ACAAATCTTT TGTATCCTGT GTATCAAGTA CCAGGCCCCC GAGCGCCAGA GAACAACACA 
GGGGCTCTCC CAGAAAAGTT ACGCGATTTA GATAGAAGAA AAAGAACGTG GAGAACTAAT 
CTTTTATTTC CCATTTATCA ATCACCTGGT CCTAGATCCC CAAGATCAGA CATTGGTGGT 
AAACCAGGTT ACTTCAAAGA AGGATTCTTG ACCTTACAAC TAGTCATGAC GTACATTTTT 
GCAATTGGCG GACACCTTGA AGCGCAAAAT CTAACACAGT TTCAAAATAT TATTACTACA 
CTTCTGCCAC CGCTAATTAA AATGCAACGT TTTCCGTATG GAAGTTGGAT AGAGGATCCT 
TTGCTACAAG CTTTAGAAAG TTTCGTTGGA GTTATTATTA TGTTGAGTTT CGTTTACACT 
TGCATAAACA CGGTTAAAGT TATAACAACC GAAAAAGAGA AACAGCTTAA GGAGGCTATG 
AAAATTATGG GTTTGCCTAA CTGGCTCCAT TGGACTGCGT GGTTTATCAA AACATTTGTC 
TTTCTTTTAG TTTCAGTAGT TTTAATTATT TTACTTTTGA AAATCTCGTG GTATTCACAC 
ACCGAATATA GTGTTTTCAC GTACGCAGAT CCCACAGTAA TGTTGGTGTT CTTAGTACTG 
TACGTTTGTG CAACTATAAC TTTTTGTTTT GCGATTAGCG TATTTTTTTC TAAAGCAAAT 
ACAGCTGCAA CAGTAGCTGG TCTTGTATGG TTCCTCTCTT ATGCTCCATA TTTATTTTTA 
CAAAAGCAGT ATGACCAACT CAGTCTCTCT ACTAAACTCA TAGCAAGTCT TGGGTCAAAC 
ACTGCAATGG CTTACGGGTT CCAGTTAATG TTAATGTATG AGGGTACCAG TGAAGGAATA 
CAATGGTCCA ATATAGGAAG ACCCAATACT CCCGATGACA GTCTAACGCT CGGTTACATA 
ATGATGATGC TGGTAATAGA CACTTTTATC TATCTTTTTG TGGCTCTTTA CGTCGAAGCC 
GTTTTTCCCG GGGAGTACGG AGTGCCACTT CCTTGGTACT TCCCGTTTAC TTCCGCCTAT 
TGGTGTGGTC ATCCTAGCTA TGTAGGAGTT GAAGATTTCA CTAGTGTCCC ATCACAAGAA 
GGTGAATTTT TTGAAAAAGA ACCTAACAAT CTTACACCCG GTATACAAAT TCGAAATTTG 
CGGAAAGTTT TTGACCAAAA AGCAGCAGTT CGAAATCTCT CGCTAAACAT GTACAACGAT 
CAAATTACGG TCCTTCTTGG ACACAATGGT GCCGGAAAAA CTACCACCAT GTCCATGCTG 
ACTGGAATGT TTCCTCCCAC AAGTGGTACT GCAGTCGTTT GTGGACACGA CATTAAAACG 
GATATGGACG GAGTAAGAGA AAGTCTGGGT CTATGTCCTC AACATAACAT CATCTTCGAC 
GAACTCACCG TCCGAGAGCA TCTCTACTTT TTCAGTAAAC TTAAAGGACT GAAACGTGAA 
GCAATCAATG AAGAAATTGA TAAATACGTC GAACTGTTGG AACTGCAACC TAAAGCGAAT 
GCAAAATCGG CCACTTTGTC AGGAGGAATG AAGAGAAAAC TTTGTGTGGG TATGGCATTG 
TGTGGCAACT CTAAAGTTGT CATGCTTGAT GAACCCACTG CCGGAATGGA TCCATCAGCA 
AGACGAGCTT TGTGGGATTT GCTTCAAAAT CAAAAACACG GAAGGACGAT GCTTTTAACA 
ACACATTTTA TGGACGAAGC TGATTTATTA GGTGATAGAA TCGCTATTAT GGCCGGAGGG 
GAGTTACAGT GTTGCGGCTC AAGTTTCTTC CTAAAGAAGA AATACGGGAC GGGATACCAT 
CTCATAATGG ATAAGGCTTC GAATTGTCAA GAGGAACAAG TAACACAACT TTTGAAGCGA 
CATATTCCTA ATATCCAGGT ACACGGTAAT GTTGGCTCTG AACTCACGTA TCTACTATCC 
GAAGATCACT CTTCAGTTTT TGAAGAAATG CTGCGAGACT TAGAAGAAAA TAGTGATAGT 
TTGGGTATTC GTAGTTACGG AATTTCACTA ACAACTTTAG AAGAAGTTTT CATGAAAGTC 
GGTGCTGACC ACGGCCAGGA AGAAAGCTAT AACGGTTTTG TGCAAGAAAC AGATTGTAAT 
AAAACAATGC TGGGTAAAAT AAAATCGAAA TTGAAAAGGA ATGAAAACTA CGAAAATAGC 
ATTAACGAAA CAACTTTAAA CCTTGAAACT TCTGCTCTTC TAACCGGTTT CATGTTAATC 
AAAAATCAGT TTTTGGCAAT GCTTATGAAA AAAATTTTAT CAATCGTACG AACCTGGATA 
TTGCAGTTTA TTCAAATTTT GATGCCAGTT GCGTTTCTTA TCATAGCCAT TGTCGTTAGC 
AGAAACACTA ACAAATCAGC AGATTTACCC AAACTGCCCA TAACATTAGA TTCGTATCGC 
AATCCAGTAA CGCTAATTGA AGATAATGCA AATAACTCAT ATTCGGCCTT GTATATGCAA 
AGCCTTAAAG GTTATGAAAT TCAAAGAGTG GCAAGCATCC ACGATGTAAT GCTTAATTTG 
ACCGTAACTG TTCCATCCAC TGTCAGACTA AGATACATTG TTGGAGCTAG TTTCAACAAA 
ACATTCGGCG GTGTTTTTAC AACTATCACA GCCTGGTTTA ATAATAACCC TTACCACTCC 
CCGCCTCTAG CATTAGGTCT TGCCTTAAAC GTGGCTTTCA AGCATTTAGC TGGCACAAAT 
CGTTCAATAA GATGGGCAAA TTATCCGTTA CCGTTTACAG CCGATACAAA AGTGCAGCAA 
CTGTTGGAAG GCAACAGTAT GGGTTTCCAG CTCGCTTTCA ACATCGGATT TAGTATGGCT 
TTTGTATCCT CATTCTACGT CCTATTTTAT GTGCGGGAAC GAGTTTGCAA GTCAAAACAT 
TTACAATTCG TGTCCGGAGT GAAAGTTTAC ATATTCTGGA TAATGTCATT CGTCTGTGAT 
ATAATCACGT TCATTGTGAC CATCATCGCG ATCATCATTA CTTTGGCGTG TTTCCAAGAA 
GATGGCTTCC GGTCGGCTGA GGACTTAGGT CGCATGTTCC TTCTTTTGTT CTATTTTGGC 
GTTGCAATGT TGCCTATGAT GTACTTTGCG TCATACTTGT TCGAAGTACC TTCGACAGGT 
TATACTAGAA TGACCCTCTT TAGTGTTTTC ACTGGAGTGG CTGCCTTTTT GGTTGTTCAA 
GTTTTGGCAA CACCTGGATT GGATTTGGAA TATGTCGCGA ATACTCTACA TTGGATTTTC 
CTGGTAGTAC CCCATTATTC GTTAGCTACA GGTATTAGAG ACGTTTATAC AACATTTGCC 
ACTAATAAAA TGTGCAAATA CGCCGTCCAA ACGTGCGTCG AGAATAGGCC AGATATGACT 
GTGGACAAGT GTTGGGATTT GACTTGTCAT AATGCAAGTG CTGTGCTACA GGATTACTGT 
TGTGTACGCG AGCCGCACTA TTACAAATGG GCCAGTCCTG GAATAGGCCG AAATTTGCTA 
TACTCATTTT CCGTTGGAAT AGTCCTATTT ATTCTACTCA ACATAAAAGA ATATAAAATA 
TTTTCAAAAA CGATAGACCA TATTGTTGAG AGCTATAGAA ACAAATTGCC CCAGCCAGTG 
GAAAATGAAG ACAGCGATGT CGCGGAAGAA AAACACAAAA TCCACAACAC TTCTGAAACA 
GAGTTACAAA AAGACTACAT TTTGGTGCTT AAAGATTTGA CAAAGTATTA CAACAACTTT 
TTGGCAGTTA ACGGATTGTG TCTGGGTGTT AAGGAACGTG AATGCTTCGG ATTATTGGGT 
ATAAACGGTG CTGGGAAAAC CACAACTTTT AAAATGATGA CCGGTGATGT AAAAATCACG 
CACGGAGAAG CTTGGGTGAA AAAATTCAGT ATCAAATCGC AACTAAAACA AGTGCAAAAG 
TTGATTGGAT ATTGTCCGCA ATTTGACGCT CTTCTTGATG ACCTGACTGC CAAAGAATCA 
TTGGTAATGT TTGCACTTTT GAGAGGAGTT CCAATGGACG AGTGCCGATA CCTGGTCCAG 
AAACTATCGA CAGATTTTGA TTTCCAAAGA CATTTGAATA AAAAGGTTAA GGAACTTAGT 
GGTGGTAACA AGAGGAAGTT AAGTACTGCC ATTTCGCTGA TTGGCGATCC TCCGGTGATA 
TATTTAGACG AACCTACGAC AGGAATGGAC CCTGCGACGA AACGCTATTT GTGGGATGCC 
ATCTGCAAAG TTAGAGATAA CGGAAAATGT GTAGTCTTGA CCTCTCATAG TATGGAAGAA 
TGTGAAGCTT TGTGCACAAG ACTAGCCATA ATGGTTAATG GCAATTTCAA ATGTTTGGGT 
TCCACTCAAC ATTTGAAAAA TAAATTTGCC GAAGGGTATA CGTTGACAAT TAAAATTAAG 
AAAAGCCCTG GCGGGGAAGA TGCCGATATT AAACCAATTG AAAATTTCAT CTCGGACCAT 
TTTCCCAAAG CAATACAAAG AGAAAAACAT CAAGAGCTTT TAACATATTA TATTGTAGAT 
AAAAGCATCC CCTGGTCCAG AATGTTCGGG ATTTTGGAAA AAGGAAAAAA GCAGCTAAAT 
ATTGAAGATT ATTCTTTGGG TCAATCAAGT TTGGAACAGG TATTTTTATC TTTCACAAGG 
TTGCAACGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015840358.1
CDS	182..5413
Translation	MGKKLDKFLLLMWKNWLLQYRKPLQTLVEILAPVLFSILLVVIRSLVDPETNDPKIYTPFCLLPILCSDESISTNPKFLTNLGNTTFRNFTLVYSPYSNKAVRRSMGILETVLGGHVVGYNNSDQLEAHFLDSNSSLTFAAIQLDDSLAHQDTLPDDLEVSLRFPSELRIGFDLFGQNWHTNLLYPVYQVPGPRAPENNTGALPEKLRDLDRRKRTWRTNLLFPIYQSPGPRSPRSDIGGKPGYFKEGFLTLQLVMTYIFAIGGHLEAQNLTQFQNIITTLLPPLIKMQRFPYGSWIEDPLLQALESFVGVIIMLSFVYTCINTVKVITTEKEKQLKEAMKIMGLPNWLHWTAWFIKTFVFLLVSVVLIILLLKISWYSHTEYSVFTYADPTVMLVFLVLYVCATITFCFAISVFFSKANTAATVAGLVWFLSYAPYLFLQKQYDQLSLSTKLIASLGSNTAMAYGFQLMLMYEGTSEGIQWSNIGRPNTPDDSLTLGYIMMMLVIDTFIYLFVALYVEAVFPGEYGVPLPWYFPFTSAYWCGHPSYVGVEDFTSVPSQEGEFFEKEPNNLTPGIQIRNLRKVFDQKAAVRNLSLNMYNDQITVLLGHNGAGKTTTMSMLTGMFPPTSGTAVVCGHDIKTDMDGVRESLGLCPQHNIIFDELTVREHLYFFSKLKGLKREAINEEIDKYVELLELQPKANAKSATLSGGMKRKLCVGMALCGNSKVVMLDEPTAGMDPSARRALWDLLQNQKHGRTMLLTTHFMDEADLLGDRIAIMAGGELQCCGSSFFLKKKYGTGYHLIMDKASNCQEEQVTQLLKRHIPNIQVHGNVGSELTYLLSEDHSSVFEEMLRDLEENSDSLGIRSYGISLTTLEEVFMKVGADHGQEESYNGFVQETDCNKTMLGKIKSKLKRNENYENSINETTLNLETSALLTGFMLIKNQFLAMLMKKILSIVRTWILQFIQILMPVAFLIIAIVVSRNTNKSADLPKLPITLDSYRNPVTLIEDNANNSYSALYMQSLKGYEIQRVASIHDVMLNLTVTVPSTVRLRYIVGASFNKTFGGVFTTITAWFNNNPYHSPPLALGLALNVAFKHLAGTNRSIRWANYPLPFTADTKVQQLLEGNSMGFQLAFNIGFSMAFVSSFYVLFYVRERVCKSKHLQFVSGVKVYIFWIMSFVCDIITFIVTIIAIIITLACFQEDGFRSAEDLGRMFLLLFYFGVAMLPMMYFASYLFEVPSTGYTRMTLFSVFTGVAAFLVVQVLATPGLDLEYVANTLHWIFLVVPHYSLATGIRDVYTTFATNKMCKYAVQTCVENRPDMTVDKCWDLTCHNASAVLQDYCCVREPHYYKWASPGIGRNLLYSFSVGIVLFILLNIKEYKIFSKTIDHIVESYRNKLPQPVENEDSDVAEEKHKIHNTSETELQKDYILVLKDLTKYYNNFLAVNGLCLGVKERECFGLLGINGAGKTTTFKMMTGDVKITHGEAWVKKFSIKSQLKQVQKLIGYCPQFDALLDDLTAKESLVMFALLRGVPMDECRYLVQKLSTDFDFQRHLNKKVKELSGGNKRKLSTAISLIGDPPVIYLDEPTTGMDPATKRYLWDAICKVRDNGKCVVLTSHSMEECEALCTRLAIMVNGNFKCLGSTQHLKNKFAEGYTLTIKIKKSPGGEDADIKPIENFISDHFPKAIQREKHQELLTYYIVDKSIPWSRMFGILEKGKKQLNIEDYSLGQSSLEQVFLSFTRLQR

Target ORF information:

RefSeq Version	XM_015984872.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015984872.1

ORF Insert Sequence:

ATGGGGAAGA AACTGGACAA GTTCCTTCTA CTGATGTGGA AGAATTGGTT GCTGCAATAC 
CGGAAGCCTC TCCAAACCCT TGTAGAAATT CTAGCTCCAG TCCTGTTCTC CATCCTCCTA 
GTCGTTATTC GAAGTCTGGT TGATCCTGAA ACGAATGATC CAAAGATTTA CACGCCCTTC 
TGTCTACTCC CAATTTTATG CTCGGATGAA AGTATTAGCA CAAATCCGAA ATTTCTAACG 
AACCTCGGAA ATACGACGTT CAGAAACTTT ACACTAGTGT ATTCACCTTA CTCAAACAAA 
GCCGTAAGAC GGTCAATGGG AATTCTTGAA ACTGTTTTGG GTGGCCACGT GGTTGGTTAC 
AACAACTCTG ACCAACTAGA AGCCCATTTC CTCGATTCGA ATTCCTCTCT CACGTTTGCT 
GCCATCCAAC TTGATGACTC TTTAGCGCAC CAAGACACTC TACCAGACGA CTTGGAAGTA 
TCGCTGAGAT TTCCAAGCGA ACTTCGGATT GGGTTCGATT TGTTTGGGCA AAACTGGCAC 
ACAAATCTTT TGTATCCTGT GTATCAAGTA CCAGGCCCCC GAGCGCCAGA GAACAACACA 
GGGGCTCTCC CAGAAAAGTT ACGCGATTTA GATAGAAGAA AAAGAACGTG GAGAACTAAT 
CTTTTATTTC CCATTTATCA ATCACCTGGT CCTAGATCCC CAAGATCAGA CATTGGTGGT 
AAACCAGGTT ACTTCAAAGA AGGATTCTTG ACCTTACAAC TAGTCATGAC GTACATTTTT 
GCAATTGGCG GACACCTTGA AGCGCAAAAT CTAACACAGT TTCAAAATAT TATTACTACA 
CTTCTGCCAC CGCTAATTAA AATGCAACGT TTTCCGTATG GAAGTTGGAT AGAGGATCCT 
TTGCTACAAG CTTTAGAAAG TTTCGTTGGA GTTATTATTA TGTTGAGTTT CGTTTACACT 
TGCATAAACA CGGTTAAAGT TATAACAACC GAAAAAGAGA AACAGCTTAA GGAGGCTATG 
AAAATTATGG GTTTGCCTAA CTGGCTCCAT TGGACTGCGT GGTTTATCAA AACATTTGTC 
TTTCTTTTAG TTTCAGTAGT TTTAATTATT TTACTTTTGA AAATCTCGTG GTATTCACAC 
ACCGAATATA GTGTTTTCAC GTACGCAGAT CCCACAGTAA TGTTGGTGTT CTTAGTACTG 
TACGTTTGTG CAACTATAAC TTTTTGTTTT GCGATTAGCG TATTTTTTTC TAAAGCAAAT 
ACAGCTGCAA CAGTAGCTGG TCTTGTATGG TTCCTCTCTT ATGCTCCATA TTTATTTTTA 
CAAAAGCAGT ATGACCAACT CAGTCTCTCT ACTAAACTCA TAGCAAGTCT TGGGTCAAAC 
ACTGCAATGG CTTACGGGTT CCAGTTAATG TTAATGTATG AGGGTACCAG TGAAGGAATA 
CAATGGTCCA ATATAGGAAG ACCCAATACT CCCGATGACA GTCTAACGCT CGGTTACATA 
ATGATGATGC TGGTAATAGA CACTTTTATC TATCTTTTTG TGGCTCTTTA CGTCGAAGCC 
GTTTTTCCCG GGGAGTACGG AGTGCCACTT CCTTGGTACT TCCCGTTTAC TTCCGCCTAT 
TGGTGTGGTC ATCCTAGCTA TGTAGGAGTT GAAGATTTCA CTAGTGTCCC ATCACAAGAA 
GGTGAATTTT TTGAAAAAGA ACCTAACAAT CTTACACCCG GTATACAAAT TCGAAATTTG 
CGGAAAGTTT TTGACCAAAA AGCAGCAGTT CGAAATCTCT CGCTAAACAT GTACAACGAT 
CAAATTACGG TCCTTCTTGG ACACAATGGT GCCGGAAAAA CTACCACCAT GTCCATGCTG 
ACTGGAATGT TTCCTCCCAC AAGTGGTACT GCAGTCGTTT GTGGACACGA CATTAAAACG 
GATATGGACG GAGTAAGAGA AAGTCTGGGT CTATGTCCTC AACATAACAT CATCTTCGAC 
GAACTCACCG TCCGAGAGCA TCTCTACTTT TTCAGTAAAC TTAAAGGACT GAAACGTGAA 
GCAATCAATG AAGAAATTGA TAAATACGTC GAACTGTTGG AACTGCAACC TAAAGCGAAT 
GCAAAATCGG CCACTTTGTC AGGAGGAATG AAGAGAAAAC TTTGTGTGGG TATGGCATTG 
TGTGGCAACT CTAAAGTTGT CATGCTTGAT GAACCCACTG CCGGAATGGA TCCATCAGCA 
AGACGAGCTT TGTGGGATTT GCTTCAAAAT CAAAAACACG GAAGGACGAT GCTTTTAACA 
ACACATTTTA TGGACGAAGC TGATTTATTA GGTGATAGAA TCGCTATTAT GGCCGGAGGG 
GAGTTACAGT GTTGCGGCTC AAGTTTCTTC CTAAAGAAGA AATACGGGAC GGGATACCAT 
CTCATAATGG ATAAGGCTTC GAATTGTCAA GAGGAACAAG TAACACAACT TTTGAAGCGA 
CATATTCCTA ATATCCAGGT ACACGGTAAT GTTGGCTCTG AACTCACGTA TCTACTATCC 
GAAGATCACT CTTCAGTTTT TGAAGAAATG CTGCGAGACT TAGAAGAAAA TAGTGATAGT 
TTGGGTATTC GTAGTTACGG AATTTCACTA ACAACTTTAG AAGAAGTTTT CATGAAAGTC 
GGTGCTGACC ACGGCCAGGA AGAAAGCTAT AACGGTTTTG TGCAAGAAAC AGATTGTAAT 
AAAACAATGC TGGGTAAAAT AAAATCGAAA TTGAAAAGGA ATGAAAACTA CGAAAATAGC 
ATTAACGAAA CAACTTTAAA CCTTGAAACT TCTGCTCTTC TAACCGGTTT CATGTTAATC 
AAAAATCAGT TTTTGGCAAT GCTTATGAAA AAAATTTTAT CAATCGTACG AACCTGGATA 
TTGCAGTTTA TTCAAATTTT GATGCCAGTT GCGTTTCTTA TCATAGCCAT TGTCGTTAGC 
AGAAACACTA ACAAATCAGC AGATTTACCC AAACTGCCCA TAACATTAGA TTCGTATCGC 
AATCCAGTAA CGCTAATTGA AGATAATGCA AATAACTCAT ATTCGGCCTT GTATATGCAA 
AGCCTTAAAG GTTATGAAAT TCAAAGAGTG GCAAGCATCC ACGATGTAAT GCTTAATTTG 
ACCGTAACTG TTCCATCCAC TGTCAGACTA AGATACATTG TTGGAGCTAG TTTCAACAAA 
ACATTCGGCG GTGTTTTTAC AACTATCACA GCCTGGTTTA ATAATAACCC TTACCACTCC 
CCGCCTCTAG CATTAGGTCT TGCCTTAAAC GTGGCTTTCA AGCATTTAGC TGGCACAAAT 
CGTTCAATAA GATGGGCAAA TTATCCGTTA CCGTTTACAG CCGATACAAA AGTGCAGCAA 
CTGTTGGAAG GCAACAGTAT GGGTTTCCAG CTCGCTTTCA ACATCGGATT TAGTATGGCT 
TTTGTATCCT CATTCTACGT CCTATTTTAT GTGCGGGAAC GAGTTTGCAA GTCAAAACAT 
TTACAATTCG TGTCCGGAGT GAAAGTTTAC ATATTCTGGA TAATGTCATT CGTCTGTGAT 
ATAATCACGT TCATTGTGAC CATCATCGCG ATCATCATTA CTTTGGCGTG TTTCCAAGAA 
GATGGCTTCC GGTCGGCTGA GGACTTAGGT CGCATGTTCC TTCTTTTGTT CTATTTTGGC 
GTTGCAATGT TGCCTATGAT GTACTTTGCG TCATACTTGT TCGAAGTACC TTCGACAGGT 
TATACTAGAA TGACCCTCTT TAGTGTTTTC ACTGGAGTGG CTGCCTTTTT GGTTGTTCAA 
GTTTTGGCAA CACCTGGATT GGATTTGGAA TATGTCGCGA ATACTCTACA TTGGATTTTC 
CTGGTAGTAC CCCATTATTC GTTAGCTACA GGTATTAGAG ACGTTTATAC AACATTTGCC 
ACTAATAAAA TGTGCAAATA CGCCGTCCAA ACGTGCGTCG AGAATAGGCC AGATATGACT 
GTGGACAAGT GTTGGGATTT GACTTGTCAT AATGCAAGTG CTGTGCTACA GGATTACTGT 
TGTGTACGCG AGCCGCACTA TTACAAATGG GCCAGTCCTG GAATAGGCCG AAATTTGCTA 
TACTCATTTT CCGTTGGAAT AGTCCTATTT ATTCTACTCA ACATAAAAGA ATATAAAATA 
TTTTCAAAAA CGATAGACCA TATTGTTGAG AGCTATAGAA ACAAATTGCC CCAGCCAGTG 
GAAAATGAAG ACAGCGATGT CGCGGAAGAA AAACACAAAA TCCACAACAC TTCTGAAACA 
GAGTTACAAA AAGACTACAT TTTGGTGCTT AAAGATTTGA CAAAGTATTA CAACAACTTT 
TTGGCAGTTA ACGGATTGTG TCTGGGTGTT AAGGAACGTG AATGCTTCGG ATTATTGGGT 
ATAAACGGTG CTGGGAAAAC CACAACTTTT AAAATGATGA CCGGTGATGT AAAAATCACG 
CACGGAGAAG CTTGGGTGAA AAAATTCAGT ATCAAATCGC AACTAAAACA AGTGCAAAAG 
TTGATTGGAT ATTGTCCGCA ATTTGACGCT CTTCTTGATG ACCTGACTGC CAAAGAATCA 
TTGGTAATGT TTGCACTTTT GAGAGGAGTT CCAATGGACG AGTGCCGATA CCTGGTCCAG 
AAACTATCGA CAGATTTTGA TTTCCAAAGA CATTTGAATA AAAAGGTTAA GGAACTTAGT 
GGTGGTAACA AGAGGAAGTT AAGTACTGCC ATTTCGCTGA TTGGCGATCC TCCGGTGATA 
TATTTAGACG AACCTACGAC AGGAATGGAC CCTGCGACGA AACGCTATTT GTGGGATGCC 
ATCTGCAAAG TTAGAGATAA CGGAAAATGT GTAGTCTTGA CCTCTCATAG TATGGAAGAA 
TGTGAAGCTT TGTGCACAAG ACTAGCCATA ATGGTTAATG GCAATTTCAA ATGTTTGGGT 
TCCACTCAAC ATTTGAAAAA TAAATTTGCC GAAGGGTATA CGTTGACAAT TAAAATTAAG 
AAAAGCCCTG GCGGGGAAGA TGCCGATATT AAACCAATTG AAAATTTCAT CTCGGACCAT 
TTTCCCAAAG CAATACAAAG AGAAAAACAT CAAGAGCTTT TAACATATTA TATTGTAGAT 
AAAAGCATCC CCTGGTCCAG AATGTTCGGG ATTTTGGAAA AAGGAAAAAA GCAGCTAAAT 
ATTGAAGATT ATTCTTTGGG TCAATCAAGT TTGGAACAGG TATTTTTATC TTTCACAAGG 
TTGCAACGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC659344 ( XM_008200930.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008199152.1 Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X3, mRNA.

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CloneID	OTg14533
Clone ID Related Accession (Same CDS sequence)	XM_015984873.1 , XM_008200929.2 , XM_015984872.1 , XM_008200930.2
Accession Version	XM_008200930.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	ATP-binding cassette sub-family A member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015451937.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008200930.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGA AACTGGACAA GTTCCTTCTA CTGATGTGGA AGAATTGGTT GCTGCAATAC 
CGGAAGCCTC TCCAAACCCT TGTAGAAATT CTAGCTCCAG TCCTGTTCTC CATCCTCCTA 
GTCGTTATTC GAAGTCTGGT TGATCCTGAA ACGAATGATC CAAAGATTTA CACGCCCTTC 
TGTCTACTCC CAATTTTATG CTCGGATGAA AGTATTAGCA CAAATCCGAA ATTTCTAACG 
AACCTCGGAA ATACGACGTT CAGAAACTTT ACACTAGTGT ATTCACCTTA CTCAAACAAA 
GCCGTAAGAC GGTCAATGGG AATTCTTGAA ACTGTTTTGG GTGGCCACGT GGTTGGTTAC 
AACAACTCTG ACCAACTAGA AGCCCATTTC CTCGATTCGA ATTCCTCTCT CACGTTTGCT 
GCCATCCAAC TTGATGACTC TTTAGCGCAC CAAGACACTC TACCAGACGA CTTGGAAGTA 
TCGCTGAGAT TTCCAAGCGA ACTTCGGATT GGGTTCGATT TGTTTGGGCA AAACTGGCAC 
ACAAATCTTT TGTATCCTGT GTATCAAGTA CCAGGCCCCC GAGCGCCAGA GAACAACACA 
GGGGCTCTCC CAGAAAAGTT ACGCGATTTA GATAGAAGAA AAAGAACGTG GAGAACTAAT 
CTTTTATTTC CCATTTATCA ATCACCTGGT CCTAGATCCC CAAGATCAGA CATTGGTGGT 
AAACCAGGTT ACTTCAAAGA AGGATTCTTG ACCTTACAAC TAGTCATGAC GTACATTTTT 
GCAATTGGCG GACACCTTGA AGCGCAAAAT CTAACACAGT TTCAAAATAT TATTACTACA 
CTTCTGCCAC CGCTAATTAA AATGCAACGT TTTCCGTATG GAAGTTGGAT AGAGGATCCT 
TTGCTACAAG CTTTAGAAAG TTTCGTTGGA GTTATTATTA TGTTGAGTTT CGTTTACACT 
TGCATAAACA CGGTTAAAGT TATAACAACC GAAAAAGAGA AACAGCTTAA GGAGGCTATG 
AAAATTATGG GTTTGCCTAA CTGGCTCCAT TGGACTGCGT GGTTTATCAA AACATTTGTC 
TTTCTTTTAG TTTCAGTAGT TTTAATTATT TTACTTTTGA AAATCTCGTG GTATTCACAC 
ACCGAATATA GTGTTTTCAC GTACGCAGAT CCCACAGTAA TGTTGGTGTT CTTAGTACTG 
TACGTTTGTG CAACTATAAC TTTTTGTTTT GCGATTAGCG TATTTTTTTC TAAAGCAAAT 
ACAGCTGCAA CAGTAGCTGG TCTTGTATGG TTCCTCTCTT ATGCTCCATA TTTATTTTTA 
CAAAAGCAGT ATGACCAACT CAGTCTCTCT ACTAAACTCA TAGCAAGTCT TGGGTCAAAC 
ACTGCAATGG CTTACGGGTT CCAGTTAATG TTAATGTATG AGGGTACCAG TGAAGGAATA 
CAATGGTCCA ATATAGGAAG ACCCAATACT CCCGATGACA GTCTAACGCT CGGTTACATA 
ATGATGATGC TGGTAATAGA CACTTTTATC TATCTTTTTG TGGCTCTTTA CGTCGAAGCC 
GTTTTTCCCG GGGAGTACGG AGTGCCACTT CCTTGGTACT TCCCGTTTAC TTCCGCCTAT 
TGGTGTGGTC ATCCTAGCTA TGTAGGAGTT GAAGATTTCA CTAGTGTCCC ATCACAAGAA 
GGTGAATTTT TTGAAAAAGA ACCTAACAAT CTTACACCCG GTATACAAAT TCGAAATTTG 
CGGAAAGTTT TTGACCAAAA AGCAGCAGTT CGAAATCTCT CGCTAAACAT GTACAACGAT 
CAAATTACGG TCCTTCTTGG ACACAATGGT GCCGGAAAAA CTACCACCAT GTCCATGCTG 
ACTGGAATGT TTCCTCCCAC AAGTGGTACT GCAGTCGTTT GTGGACACGA CATTAAAACG 
GATATGGACG GAGTAAGAGA AAGTCTGGGT CTATGTCCTC AACATAACAT CATCTTCGAC 
GAACTCACCG TCCGAGAGCA TCTCTACTTT TTCAGTAAAC TTAAAGGACT GAAACGTGAA 
GCAATCAATG AAGAAATTGA TAAATACGTC GAACTGTTGG AACTGCAACC TAAAGCGAAT 
GCAAAATCGG CCACTTTGTC AGGAGGAATG AAGAGAAAAC TTTGTGTGGG TATGGCATTG 
TGTGGCAACT CTAAAGTTGT CATGCTTGAT GAACCCACTG CCGGAATGGA TCCATCAGCA 
AGACGAGCTT TGTGGGATTT GCTTCAAAAT CAAAAACACG GAAGGACGAT GCTTTTAACA 
ACACATTTTA TGGACGAAGC TGATTTATTA GGTGATAGAA TCGCTATTAT GGCCGGAGGG 
GAGTTACAGT GTTGCGGCTC AAGTTTCTTC CTAAAGAAGA AATACGGGAC GGGATACCAT 
CTCATAATGG ATAAGGCTTC GAATTGTCAA GAGGAACAAG TAACACAACT TTTGAAGCGA 
CATATTCCTA ATATCCAGGT ACACGGTAAT GTTGGCTCTG AACTCACGTA TCTACTATCC 
GAAGATCACT CTTCAGTTTT TGAAGAAATG CTGCGAGACT TAGAAGAAAA TAGTGATAGT 
TTGGGTATTC GTAGTTACGG AATTTCACTA ACAACTTTAG AAGAAGTTTT CATGAAAGTC 
GGTGCTGACC ACGGCCAGGA AGAAAGCTAT AACGGTTTTG TGCAAGAAAC AGATTGTAAT 
AAAACAATGC TGGGTAAAAT AAAATCGAAA TTGAAAAGGA ATGAAAACTA CGAAAATAGC 
ATTAACGAAA CAACTTTAAA CCTTGAAACT TCTGCTCTTC TAACCGGTTT CATGTTAATC 
AAAAATCAGT TTTTGGCAAT GCTTATGAAA AAAATTTTAT CAATCGTACG AACCTGGATA 
TTGCAGTTTA TTCAAATTTT GATGCCAGTT GCGTTTCTTA TCATAGCCAT TGTCGTTAGC 
AGAAACACTA ACAAATCAGC AGATTTACCC AAACTGCCCA TAACATTAGA TTCGTATCGC 
AATCCAGTAA CGCTAATTGA AGATAATGCA AATAACTCAT ATTCGGCCTT GTATATGCAA 
AGCCTTAAAG GTTATGAAAT TCAAAGAGTG GCAAGCATCC ACGATGTAAT GCTTAATTTG 
ACCGTAACTG TTCCATCCAC TGTCAGACTA AGATACATTG TTGGAGCTAG TTTCAACAAA 
ACATTCGGCG GTGTTTTTAC AACTATCACA GCCTGGTTTA ATAATAACCC TTACCACTCC 
CCGCCTCTAG CATTAGGTCT TGCCTTAAAC GTGGCTTTCA AGCATTTAGC TGGCACAAAT 
CGTTCAATAA GATGGGCAAA TTATCCGTTA CCGTTTACAG CCGATACAAA AGTGCAGCAA 
CTGTTGGAAG GCAACAGTAT GGGTTTCCAG CTCGCTTTCA ACATCGGATT TAGTATGGCT 
TTTGTATCCT CATTCTACGT CCTATTTTAT GTGCGGGAAC GAGTTTGCAA GTCAAAACAT 
TTACAATTCG TGTCCGGAGT GAAAGTTTAC ATATTCTGGA TAATGTCATT CGTCTGTGAT 
ATAATCACGT TCATTGTGAC CATCATCGCG ATCATCATTA CTTTGGCGTG TTTCCAAGAA 
GATGGCTTCC GGTCGGCTGA GGACTTAGGT CGCATGTTCC TTCTTTTGTT CTATTTTGGC 
GTTGCAATGT TGCCTATGAT GTACTTTGCG TCATACTTGT TCGAAGTACC TTCGACAGGT 
TATACTAGAA TGACCCTCTT TAGTGTTTTC ACTGGAGTGG CTGCCTTTTT GGTTGTTCAA 
GTTTTGGCAA CACCTGGATT GGATTTGGAA TATGTCGCGA ATACTCTACA TTGGATTTTC 
CTGGTAGTAC CCCATTATTC GTTAGCTACA GGTATTAGAG ACGTTTATAC AACATTTGCC 
ACTAATAAAA TGTGCAAATA CGCCGTCCAA ACGTGCGTCG AGAATAGGCC AGATATGACT 
GTGGACAAGT GTTGGGATTT GACTTGTCAT AATGCAAGTG CTGTGCTACA GGATTACTGT 
TGTGTACGCG AGCCGCACTA TTACAAATGG GCCAGTCCTG GAATAGGCCG AAATTTGCTA 
TACTCATTTT CCGTTGGAAT AGTCCTATTT ATTCTACTCA ACATAAAAGA ATATAAAATA 
TTTTCAAAAA CGATAGACCA TATTGTTGAG AGCTATAGAA ACAAATTGCC CCAGCCAGTG 
GAAAATGAAG ACAGCGATGT CGCGGAAGAA AAACACAAAA TCCACAACAC TTCTGAAACA 
GAGTTACAAA AAGACTACAT TTTGGTGCTT AAAGATTTGA CAAAGTATTA CAACAACTTT 
TTGGCAGTTA ACGGATTGTG TCTGGGTGTT AAGGAACGTG AATGCTTCGG ATTATTGGGT 
ATAAACGGTG CTGGGAAAAC CACAACTTTT AAAATGATGA CCGGTGATGT AAAAATCACG 
CACGGAGAAG CTTGGGTGAA AAAATTCAGT ATCAAATCGC AACTAAAACA AGTGCAAAAG 
TTGATTGGAT ATTGTCCGCA ATTTGACGCT CTTCTTGATG ACCTGACTGC CAAAGAATCA 
TTGGTAATGT TTGCACTTTT GAGAGGAGTT CCAATGGACG AGTGCCGATA CCTGGTCCAG 
AAACTATCGA CAGATTTTGA TTTCCAAAGA CATTTGAATA AAAAGGTTAA GGAACTTAGT 
GGTGGTAACA AGAGGAAGTT AAGTACTGCC ATTTCGCTGA TTGGCGATCC TCCGGTGATA 
TATTTAGACG AACCTACGAC AGGAATGGAC CCTGCGACGA AACGCTATTT GTGGGATGCC 
ATCTGCAAAG TTAGAGATAA CGGAAAATGT GTAGTCTTGA CCTCTCATAG TATGGAAGAA 
TGTGAAGCTT TGTGCACAAG ACTAGCCATA ATGGTTAATG GCAATTTCAA ATGTTTGGGT 
TCCACTCAAC ATTTGAAAAA TAAATTTGCC GAAGGGTATA CGTTGACAAT TAAAATTAAG 
AAAAGCCCTG GCGGGGAAGA TGCCGATATT AAACCAATTG AAAATTTCAT CTCGGACCAT 
TTTCCCAAAG CAATACAAAG AGAAAAACAT CAAGAGCTTT TAACATATTA TATTGTAGAT 
AAAAGCATCC CCTGGTCCAG AATGTTCGGG ATTTTGGAAA AAGGAAAAAA GCAGCTAAAT 
ATTGAAGATT ATTCTTTGGG TCAATCAAGT TTGGAACAGG TATTTTTATC TTTCACAAGG 
TTGCAACGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008199152.1
CDS	202..5433
Translation	MGKKLDKFLLLMWKNWLLQYRKPLQTLVEILAPVLFSILLVVIRSLVDPETNDPKIYTPFCLLPILCSDESISTNPKFLTNLGNTTFRNFTLVYSPYSNKAVRRSMGILETVLGGHVVGYNNSDQLEAHFLDSNSSLTFAAIQLDDSLAHQDTLPDDLEVSLRFPSELRIGFDLFGQNWHTNLLYPVYQVPGPRAPENNTGALPEKLRDLDRRKRTWRTNLLFPIYQSPGPRSPRSDIGGKPGYFKEGFLTLQLVMTYIFAIGGHLEAQNLTQFQNIITTLLPPLIKMQRFPYGSWIEDPLLQALESFVGVIIMLSFVYTCINTVKVITTEKEKQLKEAMKIMGLPNWLHWTAWFIKTFVFLLVSVVLIILLLKISWYSHTEYSVFTYADPTVMLVFLVLYVCATITFCFAISVFFSKANTAATVAGLVWFLSYAPYLFLQKQYDQLSLSTKLIASLGSNTAMAYGFQLMLMYEGTSEGIQWSNIGRPNTPDDSLTLGYIMMMLVIDTFIYLFVALYVEAVFPGEYGVPLPWYFPFTSAYWCGHPSYVGVEDFTSVPSQEGEFFEKEPNNLTPGIQIRNLRKVFDQKAAVRNLSLNMYNDQITVLLGHNGAGKTTTMSMLTGMFPPTSGTAVVCGHDIKTDMDGVRESLGLCPQHNIIFDELTVREHLYFFSKLKGLKREAINEEIDKYVELLELQPKANAKSATLSGGMKRKLCVGMALCGNSKVVMLDEPTAGMDPSARRALWDLLQNQKHGRTMLLTTHFMDEADLLGDRIAIMAGGELQCCGSSFFLKKKYGTGYHLIMDKASNCQEEQVTQLLKRHIPNIQVHGNVGSELTYLLSEDHSSVFEEMLRDLEENSDSLGIRSYGISLTTLEEVFMKVGADHGQEESYNGFVQETDCNKTMLGKIKSKLKRNENYENSINETTLNLETSALLTGFMLIKNQFLAMLMKKILSIVRTWILQFIQILMPVAFLIIAIVVSRNTNKSADLPKLPITLDSYRNPVTLIEDNANNSYSALYMQSLKGYEIQRVASIHDVMLNLTVTVPSTVRLRYIVGASFNKTFGGVFTTITAWFNNNPYHSPPLALGLALNVAFKHLAGTNRSIRWANYPLPFTADTKVQQLLEGNSMGFQLAFNIGFSMAFVSSFYVLFYVRERVCKSKHLQFVSGVKVYIFWIMSFVCDIITFIVTIIAIIITLACFQEDGFRSAEDLGRMFLLLFYFGVAMLPMMYFASYLFEVPSTGYTRMTLFSVFTGVAAFLVVQVLATPGLDLEYVANTLHWIFLVVPHYSLATGIRDVYTTFATNKMCKYAVQTCVENRPDMTVDKCWDLTCHNASAVLQDYCCVREPHYYKWASPGIGRNLLYSFSVGIVLFILLNIKEYKIFSKTIDHIVESYRNKLPQPVENEDSDVAEEKHKIHNTSETELQKDYILVLKDLTKYYNNFLAVNGLCLGVKERECFGLLGINGAGKTTTFKMMTGDVKITHGEAWVKKFSIKSQLKQVQKLIGYCPQFDALLDDLTAKESLVMFALLRGVPMDECRYLVQKLSTDFDFQRHLNKKVKELSGGNKRKLSTAISLIGDPPVIYLDEPTTGMDPATKRYLWDAICKVRDNGKCVVLTSHSMEECEALCTRLAIMVNGNFKCLGSTQHLKNKFAEGYTLTIKIKKSPGGEDADIKPIENFISDHFPKAIQREKHQELLTYYIVDKSIPWSRMFGILEKGKKQLNIEDYSLGQSSLEQVFLSFTRLQR

Target ORF information:

RefSeq Version	XM_008200930.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008200930.2

ORF Insert Sequence:

ATGGGGAAGA AACTGGACAA GTTCCTTCTA CTGATGTGGA AGAATTGGTT GCTGCAATAC 
CGGAAGCCTC TCCAAACCCT TGTAGAAATT CTAGCTCCAG TCCTGTTCTC CATCCTCCTA 
GTCGTTATTC GAAGTCTGGT TGATCCTGAA ACGAATGATC CAAAGATTTA CACGCCCTTC 
TGTCTACTCC CAATTTTATG CTCGGATGAA AGTATTAGCA CAAATCCGAA ATTTCTAACG 
AACCTCGGAA ATACGACGTT CAGAAACTTT ACACTAGTGT ATTCACCTTA CTCAAACAAA 
GCCGTAAGAC GGTCAATGGG AATTCTTGAA ACTGTTTTGG GTGGCCACGT GGTTGGTTAC 
AACAACTCTG ACCAACTAGA AGCCCATTTC CTCGATTCGA ATTCCTCTCT CACGTTTGCT 
GCCATCCAAC TTGATGACTC TTTAGCGCAC CAAGACACTC TACCAGACGA CTTGGAAGTA 
TCGCTGAGAT TTCCAAGCGA ACTTCGGATT GGGTTCGATT TGTTTGGGCA AAACTGGCAC 
ACAAATCTTT TGTATCCTGT GTATCAAGTA CCAGGCCCCC GAGCGCCAGA GAACAACACA 
GGGGCTCTCC CAGAAAAGTT ACGCGATTTA GATAGAAGAA AAAGAACGTG GAGAACTAAT 
CTTTTATTTC CCATTTATCA ATCACCTGGT CCTAGATCCC CAAGATCAGA CATTGGTGGT 
AAACCAGGTT ACTTCAAAGA AGGATTCTTG ACCTTACAAC TAGTCATGAC GTACATTTTT 
GCAATTGGCG GACACCTTGA AGCGCAAAAT CTAACACAGT TTCAAAATAT TATTACTACA 
CTTCTGCCAC CGCTAATTAA AATGCAACGT TTTCCGTATG GAAGTTGGAT AGAGGATCCT 
TTGCTACAAG CTTTAGAAAG TTTCGTTGGA GTTATTATTA TGTTGAGTTT CGTTTACACT 
TGCATAAACA CGGTTAAAGT TATAACAACC GAAAAAGAGA AACAGCTTAA GGAGGCTATG 
AAAATTATGG GTTTGCCTAA CTGGCTCCAT TGGACTGCGT GGTTTATCAA AACATTTGTC 
TTTCTTTTAG TTTCAGTAGT TTTAATTATT TTACTTTTGA AAATCTCGTG GTATTCACAC 
ACCGAATATA GTGTTTTCAC GTACGCAGAT CCCACAGTAA TGTTGGTGTT CTTAGTACTG 
TACGTTTGTG CAACTATAAC TTTTTGTTTT GCGATTAGCG TATTTTTTTC TAAAGCAAAT 
ACAGCTGCAA CAGTAGCTGG TCTTGTATGG TTCCTCTCTT ATGCTCCATA TTTATTTTTA 
CAAAAGCAGT ATGACCAACT CAGTCTCTCT ACTAAACTCA TAGCAAGTCT TGGGTCAAAC 
ACTGCAATGG CTTACGGGTT CCAGTTAATG TTAATGTATG AGGGTACCAG TGAAGGAATA 
CAATGGTCCA ATATAGGAAG ACCCAATACT CCCGATGACA GTCTAACGCT CGGTTACATA 
ATGATGATGC TGGTAATAGA CACTTTTATC TATCTTTTTG TGGCTCTTTA CGTCGAAGCC 
GTTTTTCCCG GGGAGTACGG AGTGCCACTT CCTTGGTACT TCCCGTTTAC TTCCGCCTAT 
TGGTGTGGTC ATCCTAGCTA TGTAGGAGTT GAAGATTTCA CTAGTGTCCC ATCACAAGAA 
GGTGAATTTT TTGAAAAAGA ACCTAACAAT CTTACACCCG GTATACAAAT TCGAAATTTG 
CGGAAAGTTT TTGACCAAAA AGCAGCAGTT CGAAATCTCT CGCTAAACAT GTACAACGAT 
CAAATTACGG TCCTTCTTGG ACACAATGGT GCCGGAAAAA CTACCACCAT GTCCATGCTG 
ACTGGAATGT TTCCTCCCAC AAGTGGTACT GCAGTCGTTT GTGGACACGA CATTAAAACG 
GATATGGACG GAGTAAGAGA AAGTCTGGGT CTATGTCCTC AACATAACAT CATCTTCGAC 
GAACTCACCG TCCGAGAGCA TCTCTACTTT TTCAGTAAAC TTAAAGGACT GAAACGTGAA 
GCAATCAATG AAGAAATTGA TAAATACGTC GAACTGTTGG AACTGCAACC TAAAGCGAAT 
GCAAAATCGG CCACTTTGTC AGGAGGAATG AAGAGAAAAC TTTGTGTGGG TATGGCATTG 
TGTGGCAACT CTAAAGTTGT CATGCTTGAT GAACCCACTG CCGGAATGGA TCCATCAGCA 
AGACGAGCTT TGTGGGATTT GCTTCAAAAT CAAAAACACG GAAGGACGAT GCTTTTAACA 
ACACATTTTA TGGACGAAGC TGATTTATTA GGTGATAGAA TCGCTATTAT GGCCGGAGGG 
GAGTTACAGT GTTGCGGCTC AAGTTTCTTC CTAAAGAAGA AATACGGGAC GGGATACCAT 
CTCATAATGG ATAAGGCTTC GAATTGTCAA GAGGAACAAG TAACACAACT TTTGAAGCGA 
CATATTCCTA ATATCCAGGT ACACGGTAAT GTTGGCTCTG AACTCACGTA TCTACTATCC 
GAAGATCACT CTTCAGTTTT TGAAGAAATG CTGCGAGACT TAGAAGAAAA TAGTGATAGT 
TTGGGTATTC GTAGTTACGG AATTTCACTA ACAACTTTAG AAGAAGTTTT CATGAAAGTC 
GGTGCTGACC ACGGCCAGGA AGAAAGCTAT AACGGTTTTG TGCAAGAAAC AGATTGTAAT 
AAAACAATGC TGGGTAAAAT AAAATCGAAA TTGAAAAGGA ATGAAAACTA CGAAAATAGC 
ATTAACGAAA CAACTTTAAA CCTTGAAACT TCTGCTCTTC TAACCGGTTT CATGTTAATC 
AAAAATCAGT TTTTGGCAAT GCTTATGAAA AAAATTTTAT CAATCGTACG AACCTGGATA 
TTGCAGTTTA TTCAAATTTT GATGCCAGTT GCGTTTCTTA TCATAGCCAT TGTCGTTAGC 
AGAAACACTA ACAAATCAGC AGATTTACCC AAACTGCCCA TAACATTAGA TTCGTATCGC 
AATCCAGTAA CGCTAATTGA AGATAATGCA AATAACTCAT ATTCGGCCTT GTATATGCAA 
AGCCTTAAAG GTTATGAAAT TCAAAGAGTG GCAAGCATCC ACGATGTAAT GCTTAATTTG 
ACCGTAACTG TTCCATCCAC TGTCAGACTA AGATACATTG TTGGAGCTAG TTTCAACAAA 
ACATTCGGCG GTGTTTTTAC AACTATCACA GCCTGGTTTA ATAATAACCC TTACCACTCC 
CCGCCTCTAG CATTAGGTCT TGCCTTAAAC GTGGCTTTCA AGCATTTAGC TGGCACAAAT 
CGTTCAATAA GATGGGCAAA TTATCCGTTA CCGTTTACAG CCGATACAAA AGTGCAGCAA 
CTGTTGGAAG GCAACAGTAT GGGTTTCCAG CTCGCTTTCA ACATCGGATT TAGTATGGCT 
TTTGTATCCT CATTCTACGT CCTATTTTAT GTGCGGGAAC GAGTTTGCAA GTCAAAACAT 
TTACAATTCG TGTCCGGAGT GAAAGTTTAC ATATTCTGGA TAATGTCATT CGTCTGTGAT 
ATAATCACGT TCATTGTGAC CATCATCGCG ATCATCATTA CTTTGGCGTG TTTCCAAGAA 
GATGGCTTCC GGTCGGCTGA GGACTTAGGT CGCATGTTCC TTCTTTTGTT CTATTTTGGC 
GTTGCAATGT TGCCTATGAT GTACTTTGCG TCATACTTGT TCGAAGTACC TTCGACAGGT 
TATACTAGAA TGACCCTCTT TAGTGTTTTC ACTGGAGTGG CTGCCTTTTT GGTTGTTCAA 
GTTTTGGCAA CACCTGGATT GGATTTGGAA TATGTCGCGA ATACTCTACA TTGGATTTTC 
CTGGTAGTAC CCCATTATTC GTTAGCTACA GGTATTAGAG ACGTTTATAC AACATTTGCC 
ACTAATAAAA TGTGCAAATA CGCCGTCCAA ACGTGCGTCG AGAATAGGCC AGATATGACT 
GTGGACAAGT GTTGGGATTT GACTTGTCAT AATGCAAGTG CTGTGCTACA GGATTACTGT 
TGTGTACGCG AGCCGCACTA TTACAAATGG GCCAGTCCTG GAATAGGCCG AAATTTGCTA 
TACTCATTTT CCGTTGGAAT AGTCCTATTT ATTCTACTCA ACATAAAAGA ATATAAAATA 
TTTTCAAAAA CGATAGACCA TATTGTTGAG AGCTATAGAA ACAAATTGCC CCAGCCAGTG 
GAAAATGAAG ACAGCGATGT CGCGGAAGAA AAACACAAAA TCCACAACAC TTCTGAAACA 
GAGTTACAAA AAGACTACAT TTTGGTGCTT AAAGATTTGA CAAAGTATTA CAACAACTTT 
TTGGCAGTTA ACGGATTGTG TCTGGGTGTT AAGGAACGTG AATGCTTCGG ATTATTGGGT 
ATAAACGGTG CTGGGAAAAC CACAACTTTT AAAATGATGA CCGGTGATGT AAAAATCACG 
CACGGAGAAG CTTGGGTGAA AAAATTCAGT ATCAAATCGC AACTAAAACA AGTGCAAAAG 
TTGATTGGAT ATTGTCCGCA ATTTGACGCT CTTCTTGATG ACCTGACTGC CAAAGAATCA 
TTGGTAATGT TTGCACTTTT GAGAGGAGTT CCAATGGACG AGTGCCGATA CCTGGTCCAG 
AAACTATCGA CAGATTTTGA TTTCCAAAGA CATTTGAATA AAAAGGTTAA GGAACTTAGT 
GGTGGTAACA AGAGGAAGTT AAGTACTGCC ATTTCGCTGA TTGGCGATCC TCCGGTGATA 
TATTTAGACG AACCTACGAC AGGAATGGAC CCTGCGACGA AACGCTATTT GTGGGATGCC 
ATCTGCAAAG TTAGAGATAA CGGAAAATGT GTAGTCTTGA CCTCTCATAG TATGGAAGAA 
TGTGAAGCTT TGTGCACAAG ACTAGCCATA ATGGTTAATG GCAATTTCAA ATGTTTGGGT 
TCCACTCAAC ATTTGAAAAA TAAATTTGCC GAAGGGTATA CGTTGACAAT TAAAATTAAG 
AAAAGCCCTG GCGGGGAAGA TGCCGATATT AAACCAATTG AAAATTTCAT CTCGGACCAT 
TTTCCCAAAG CAATACAAAG AGAAAAACAT CAAGAGCTTT TAACATATTA TATTGTAGAT 
AAAAGCATCC CCTGGTCCAG AATGTTCGGG ATTTTGGAAA AAGGAAAAAA GCAGCTAAAT 
ATTGAAGATT ATTCTTTGGG TCAATCAAGT TTGGAACAGG TATTTTTATC TTTCACAAGG 
TTGCAACGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC659344 ( XM_008200931.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008199153.1 Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X7, mRNA.

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CloneID	OTg14534
Clone ID Related Accession (Same CDS sequence)	XM_008200931.2
Accession Version	XM_008200931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	ATP-binding cassette sub-family A member 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015451937.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008200931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTA CATCAAGAGA AAATTGTTCA TATTCATTAA TTGGACAGGA AAGAATACAA 
AGTGCTGAAA ATGATGAATT TCGTTCTGTT GGAACAATGG GGAAGAAACT GGACAAGTTC 
CTTCTACTGA TGTGGAAGAA TTGGTTGCTG CAATACCGGA AGCCTCTCCA AACCCTTGTA 
GAAATTCTAG CTCCAGTCCT GTTCTCCATC CTCCTAGTCG TTATTCGAAG TCTGGTTGAT 
CCTGAAACGA ATGATCCAAA GATTTACACG CCCTTCTGTC TACTCCCAAT TTTATGCTCG 
GATGAAAGTA TTAGCACAAA TCCGAAATTT CTAACGAACC TCGGAAATAC GACGTTCAGA 
AACTTTACAC TAGTGTATTC ACCTTACTCA AACAAAGCCG TAAGACGGTC AATGGGAATT 
CTTGAAACTG TTTTGGGTGG CCACGTGGTT GGTTACAACA ACTCTGACCA ACTAGAAGCC 
CATTTCCTCG ATTCGAATTC CTCTCTCACG TTTGCTGCCA TCCAACTTGA TGACTCTTTA 
GCGCACCAAG ACACTCTACC AGACGACTTG GAAGTATCGC TGAGATTTCC AAGCGAACTT 
CGGATTGGGT TCGATTTGTT TGGGCAAAAC TGGCACACAA ATCTTTTGTA TCCTGTGTAT 
CAAGTACCAG GCCCCCGAGC GCCAGAGAAC AACACAGGGG CTCTCCCAGG TTACTTCAAA 
GAAGGATTCT TGACCTTACA ACTAGTCATG ACGTACATTT TTGCAATTGG CGGACACCTT 
GAAGCGCAAA ATCTAACACA GTTTCAAAAT ATTATTACTA CACTTCTGCC ACCGCTAATT 
AAAATGCAAC GTTTTCCGTA TGGAAGTTGG ATAGAGGATC CTTTGCTACA AGCTTTAGAA 
AGTTTCGTTG GAGTTATTAT TATGTTGAGT TTCGTTTACA CTTGCATAAA CACGGTTAAA 
GTTATAACAA CCGAAAAAGA GAAACAGCTT AAGGAGGCTA TGAAAATTAT GGGTTTGCCT 
AACTGGCTCC ATTGGACTGC GTGGTTTATC AAAACATTTG TCTTTCTTTT AGTTTCAGTA 
GTTTTAATTA TTTTACTTTT GAAAATCTCG TGGTATTCAC ACACCGAATA TAGTGTTTTC 
ACGTACGCAG ATCCCACAGT AATGTTGGTG TTCTTAGTAC TGTACGTTTG TGCAACTATA 
ACTTTTTGTT TTGCGATTAG CGTATTTTTT TCTAAAGCAA ATACAGCTGC AACAGTAGCT 
GGTCTTGTAT GGTTCCTCTC TTATGCTCCA TATTTATTTT TACAAAAGCA GTATGACCAA 
CTCAGTCTCT CTACTAAACT CATAGCAAGT CTTGGGTCAA ACACTGCAAT GGCTTACGGG 
TTCCAGTTAA TGTTAATGTA TGAGGGTACC AGTGAAGGAA TACAATGGTC CAATATAGGA 
AGACCCAATA CTCCCGATGA CAGTCTAACG CTCGGTTACA TAATGATGAT GCTGGTAATA 
GACACTTTTA TCTATCTTTT TGTGGCTCTT TACGTCGAAG CCGTTTTTCC CGGGGAGTAC 
GGAGTGCCAC TTCCTTGGTA CTTCCCGTTT ACTTCCGCCT ATTGGTGTGG TCATCCTAGC 
TATGTAGGAG TTGAAGATTT CACTAGTGTC CCATCACAAG AAGGTGAATT TTTTGAAAAA 
GAACCTAACA ATCTTACACC CGGTATACAA ATTCGAAATT TGCGGAAAGT TTTTGACCAA 
AAAGCAGCAG TTCGAAATCT CTCGCTAAAC ATGTACAACG ATCAAATTAC GGTCCTTCTT 
GGACACAATG GTGCCGGAAA AACTACCACC ATGTCCATGC TGACTGGAAT GTTTCCTCCC 
ACAAGTGGTA CTGCAGTCGT TTGTGGACAC GACATTAAAA CGGATATGGA CGGAGTAAGA 
GAAAGTCTGG GTCTATGTCC TCAACATAAC ATCATCTTCG ACGAACTCAC CGTCCGAGAG 
CATCTCTACT TTTTCAGTAA ACTTAAAGGA CTGAAACGTG AAGCAATCAA TGAAGAAATT 
GATAAATACG TCGAACTGTT GGAACTGCAA CCTAAAGCGA ATGCAAAATC GGCCACTTTG 
TCAGGAGGAA TGAAGAGAAA ACTTTGTGTG GGTATGGCAT TGTGTGGCAA CTCTAAAGTT 
GTCATGCTTG ATGAACCCAC TGCCGGAATG GATCCATCAG CAAGACGAGC TTTGTGGGAT 
TTGCTTCAAA ATCAAAAACA CGGAAGGACG ATGCTTTTAA CAACACATTT TATGGACGAA 
GCTGATTTAT TAGGTGATAG AATCGCTATT ATGGCCGGAG GGGAGTTACA GTGTTGCGGC 
TCAAGTTTCT TCCTAAAGAA GAAATACGGG ACGGGATACC ATCTCATAAT GGATAAGGCT 
TCGAATTGTC AAGAGGAACA AGTAACACAA CTTTTGAAGC GACATATTCC TAATATCCAG 
GTACACGGTA ATGTTGGCTC TGAACTCACG TATCTACTAT CCGAAGATCA CTCTTCAGTT 
TTTGAAGAAA TGCTGCGAGA CTTAGAAGAA AATAGTGATA GTTTGGGTAT TCGTAGTTAC 
GGAATTTCAC TAACAACTTT AGAAGAAGTT TTCATGAAAG TCGGTGCTGA CCACGGCCAG 
GAAGAAAGCT ATAACGGTTT TGTGCAAGAA ACAGATTGTA ATAAAACAAT GCTGGGTAAA 
ATAAAATCGA AATTGAAAAG GAATGAAAAC TACGAAAATA GCATTAACGA AACAACTTTA 
AACCTTGAAA CTTCTGCTCT TCTAACCGGT TTCATGTTAA TCAAAAATCA GTTTTTGGCA 
ATGCTTATGA AAAAAATTTT ATCAATCGTA CGAACCTGGA TATTGCAGTT TATTCAAATT 
TTGATGCCAG TTGCGTTTCT TATCATAGCC ATTGTCGTTA GCAGAAACAC TAACAAATCA 
GCAGATTTAC CCAAACTGCC CATAACATTA GATTCGTATC GCAATCCAGT AACGCTAATT 
GAAGATAATG CAAATAACTC ATATTCGGCC TTGTATATGC AAAGCCTTAA AGGTTATGAA 
ATTCAAAGAG TGGCAAGCAT CCACGATGTA ATGCTTAATT TGACCGTAAC TGTTCCATCC 
ACTGTCAGAC TAAGATACAT TGTTGGAGCT AGTTTCAACA AAACATTCGG CGGTGTTTTT 
ACAACTATCA CAGCCTGGTT TAATAATAAC CCTTACCACT CCCCGCCTCT AGCATTAGGT 
CTTGCCTTAA ACGTGGCTTT CAAGCATTTA GCTGGCACAA ATCGTTCAAT AAGATGGGCA 
AATTATCCGT TACCGTTTAC AGCCGATACA AAAGTGCAGC AACTGTTGGA AGGCAACAGT 
ATGGGTTTCC AGCTCGCTTT CAACATCGGA TTTAGTATGG CTTTTGTATC CTCATTCTAC 
GTCCTATTTT ATGTGCGGGA ACGAGTTTGC AAGTCAAAAC ATTTACAATT CGTGTCCGGA 
GTGAAAGTTT ACATATTCTG GATAATGTCA TTCGTCTGTG ATATAATCAC GTTCATTGTG 
ACCATCATCG CGATCATCAT TACTTTGGCG TGTTTCCAAG AAGATGGCTT CCGGTCGGCT 
GAGGACTTAG GTCGCATGTT CCTTCTTTTG TTCTATTTTG GCGTTGCAAT GTTGCCTATG 
ATGTACTTTG CGTCATACTT GTTCGAAGTA CCTTCGACAG GTTATACTAG AATGACCCTC 
TTTAGTGTTT TCACTGGAGT GGCTGCCTTT TTGGTTGTTC AAGTTTTGGC AACACCTGGA 
TTGGATTTGG AATATGTCGC GAATACTCTA CATTGGATTT TCCTGGTAGT ACCCCATTAT 
TCGTTAGCTA CAGGTATTAG AGACGTTTAT ACAACATTTG CCACTAATAA AATGTGCAAA 
TACGCCGTCC AAACGTGCGT CGAGAATAGG CCAGATATGA CTGTGGACAA GTGTTGGGAT 
TTGACTTGTC ATAATGCAAG TGCTGTGCTA CAGGATTACT GTTGTGTACG CGAGCCGCAC 
TATTACAAAT GGGCCAGTCC TGGAATAGGC CGAAATTTGC TATACTCATT TTCCGTTGGA 
ATAGTCCTAT TTATTCTACT CAACATAAAA GAATATAAAA TATTTTCAAA AACGATAGAC 
CATATTGTTG AGAGCTATAG AAACAAATTG CCCCAGCCAG TGGAAAATGA AGACAGCGAT 
GTCGCGGAAG AAAAACACAA AATCCACAAC ACTTCTGAAA CAGAGTTACA AAAAGACTAC 
ATTTTGGTGC TTAAAGATTT GACAAAGTAT TACAACAACT TTTTGGCAGT TAACGGATTG 
TGTCTGGGTG TTAAGGAACG TGAATGCTTC GGATTATTGG GTATAAACGG TGCTGGGAAA 
ACCACAACTT TTAAAATGAT GACCGGTGAT GTAAAAATCA CGCACGGAGA AGCTTGGGTG 
AAAAAATTCA GTATCAAATC GCAACTAAAA CAAGTGCAAA AGTTGATTGG ATATTGTCCG 
CAATTTGACG CTCTTCTTGA TGACCTGACT GCCAAAGAAT CATTGGTAAT GTTTGCACTT 
TTGAGAGGAG TTCCAATGGA CGAGTGCCGA TACCTGGTCC AGAAACTATC GACAGATTTT 
GATTTCCAAA GACATTTGAA TAAAAAGGTT AAGGAACTTA GTGGTGGTAA CAAGAGGAAG 
TTAAGTACTG CCATTTCGCT GATTGGCGAT CCTCCGGTGA TATATTTAGA CGAACCTACG 
ACAGGAATGG ACCCTGCGAC GAAACGCTAT TTGTGGGATG CCATCTGCAA AGTTAGAGAT 
AACGGAAAAT GTGTAGTCTT GACCTCTCAT AGTATGGAAG AATGTGAAGC TTTGTGCACA 
AGACTAGCCA TAATGGTTAA TGGCAATTTC AAATGTTTGG GTTCCACTCA ACATTTGAAA 
AATAAATTTG CCGAAGGGTA TACGTTGACA ATTAAAATTA AGAAAAGCCC TGGCGGGGAA 
GATGCCGATA TTAAACCAAT TGAAAATTTC ATCTCGGACC ATTTTCCCAA AGCAATACAA 
AGAGAAAAAC ATCAAGAGCT TTTAACATAT TATATTGTAG ATAAAAGCAT CCCCTGGTCC 
AGAATGTTCG GGATTTTGGA AAAAGGAAAA AAGCAGCTAA ATATTGAAGA TTATTCTTTG 
GGTCAATCAA GTTTGGAACA GGTATTTTTA TCTTTCACAA GGTTGCAACG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008199153.1
CDS	111..5324
Translation	MASTSRENCSYSLIGQERIQSAENDEFRSVGTMGKKLDKFLLLMWKNWLLQYRKPLQTLVEILAPVLFSILLVVIRSLVDPETNDPKIYTPFCLLPILCSDESISTNPKFLTNLGNTTFRNFTLVYSPYSNKAVRRSMGILETVLGGHVVGYNNSDQLEAHFLDSNSSLTFAAIQLDDSLAHQDTLPDDLEVSLRFPSELRIGFDLFGQNWHTNLLYPVYQVPGPRAPENNTGALPGYFKEGFLTLQLVMTYIFAIGGHLEAQNLTQFQNIITTLLPPLIKMQRFPYGSWIEDPLLQALESFVGVIIMLSFVYTCINTVKVITTEKEKQLKEAMKIMGLPNWLHWTAWFIKTFVFLLVSVVLIILLLKISWYSHTEYSVFTYADPTVMLVFLVLYVCATITFCFAISVFFSKANTAATVAGLVWFLSYAPYLFLQKQYDQLSLSTKLIASLGSNTAMAYGFQLMLMYEGTSEGIQWSNIGRPNTPDDSLTLGYIMMMLVIDTFIYLFVALYVEAVFPGEYGVPLPWYFPFTSAYWCGHPSYVGVEDFTSVPSQEGEFFEKEPNNLTPGIQIRNLRKVFDQKAAVRNLSLNMYNDQITVLLGHNGAGKTTTMSMLTGMFPPTSGTAVVCGHDIKTDMDGVRESLGLCPQHNIIFDELTVREHLYFFSKLKGLKREAINEEIDKYVELLELQPKANAKSATLSGGMKRKLCVGMALCGNSKVVMLDEPTAGMDPSARRALWDLLQNQKHGRTMLLTTHFMDEADLLGDRIAIMAGGELQCCGSSFFLKKKYGTGYHLIMDKASNCQEEQVTQLLKRHIPNIQVHGNVGSELTYLLSEDHSSVFEEMLRDLEENSDSLGIRSYGISLTTLEEVFMKVGADHGQEESYNGFVQETDCNKTMLGKIKSKLKRNENYENSINETTLNLETSALLTGFMLIKNQFLAMLMKKILSIVRTWILQFIQILMPVAFLIIAIVVSRNTNKSADLPKLPITLDSYRNPVTLIEDNANNSYSALYMQSLKGYEIQRVASIHDVMLNLTVTVPSTVRLRYIVGASFNKTFGGVFTTITAWFNNNPYHSPPLALGLALNVAFKHLAGTNRSIRWANYPLPFTADTKVQQLLEGNSMGFQLAFNIGFSMAFVSSFYVLFYVRERVCKSKHLQFVSGVKVYIFWIMSFVCDIITFIVTIIAIIITLACFQEDGFRSAEDLGRMFLLLFYFGVAMLPMMYFASYLFEVPSTGYTRMTLFSVFTGVAAFLVVQVLATPGLDLEYVANTLHWIFLVVPHYSLATGIRDVYTTFATNKMCKYAVQTCVENRPDMTVDKCWDLTCHNASAVLQDYCCVREPHYYKWASPGIGRNLLYSFSVGIVLFILLNIKEYKIFSKTIDHIVESYRNKLPQPVENEDSDVAEEKHKIHNTSETELQKDYILVLKDLTKYYNNFLAVNGLCLGVKERECFGLLGINGAGKTTTFKMMTGDVKITHGEAWVKKFSIKSQLKQVQKLIGYCPQFDALLDDLTAKESLVMFALLRGVPMDECRYLVQKLSTDFDFQRHLNKKVKELSGGNKRKLSTAISLIGDPPVIYLDEPTTGMDPATKRYLWDAICKVRDNGKCVVLTSHSMEECEALCTRLAIMVNGNFKCLGSTQHLKNKFAEGYTLTIKIKKSPGGEDADIKPIENFISDHFPKAIQREKHQELLTYYIVDKSIPWSRMFGILEKGKKQLNIEDYSLGQSSLEQVFLSFTRLQR

Target ORF information:

RefSeq Version	XM_008200931.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum ATP-binding cassette sub-family A member 3 (LOC659344), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008200931.2

ORF Insert Sequence:

ATGGCATCTA CATCAAGAGA AAATTGTTCA TATTCATTAA TTGGACAGGA AAGAATACAA 
AGTGCTGAAA ATGATGAATT TCGTTCTGTT GGAACAATGG GGAAGAAACT GGACAAGTTC 
CTTCTACTGA TGTGGAAGAA TTGGTTGCTG CAATACCGGA AGCCTCTCCA AACCCTTGTA 
GAAATTCTAG CTCCAGTCCT GTTCTCCATC CTCCTAGTCG TTATTCGAAG TCTGGTTGAT 
CCTGAAACGA ATGATCCAAA GATTTACACG CCCTTCTGTC TACTCCCAAT TTTATGCTCG 
GATGAAAGTA TTAGCACAAA TCCGAAATTT CTAACGAACC TCGGAAATAC GACGTTCAGA 
AACTTTACAC TAGTGTATTC ACCTTACTCA AACAAAGCCG TAAGACGGTC AATGGGAATT 
CTTGAAACTG TTTTGGGTGG CCACGTGGTT GGTTACAACA ACTCTGACCA ACTAGAAGCC 
CATTTCCTCG ATTCGAATTC CTCTCTCACG TTTGCTGCCA TCCAACTTGA TGACTCTTTA 
GCGCACCAAG ACACTCTACC AGACGACTTG GAAGTATCGC TGAGATTTCC AAGCGAACTT 
CGGATTGGGT TCGATTTGTT TGGGCAAAAC TGGCACACAA ATCTTTTGTA TCCTGTGTAT 
CAAGTACCAG GCCCCCGAGC GCCAGAGAAC AACACAGGGG CTCTCCCAGG TTACTTCAAA 
GAAGGATTCT TGACCTTACA ACTAGTCATG ACGTACATTT TTGCAATTGG CGGACACCTT 
GAAGCGCAAA ATCTAACACA GTTTCAAAAT ATTATTACTA CACTTCTGCC ACCGCTAATT 
AAAATGCAAC GTTTTCCGTA TGGAAGTTGG ATAGAGGATC CTTTGCTACA AGCTTTAGAA 
AGTTTCGTTG GAGTTATTAT TATGTTGAGT TTCGTTTACA CTTGCATAAA CACGGTTAAA 
GTTATAACAA CCGAAAAAGA GAAACAGCTT AAGGAGGCTA TGAAAATTAT GGGTTTGCCT 
AACTGGCTCC ATTGGACTGC GTGGTTTATC AAAACATTTG TCTTTCTTTT AGTTTCAGTA 
GTTTTAATTA TTTTACTTTT GAAAATCTCG TGGTATTCAC ACACCGAATA TAGTGTTTTC 
ACGTACGCAG ATCCCACAGT AATGTTGGTG TTCTTAGTAC TGTACGTTTG TGCAACTATA 
ACTTTTTGTT TTGCGATTAG CGTATTTTTT TCTAAAGCAA ATACAGCTGC AACAGTAGCT 
GGTCTTGTAT GGTTCCTCTC TTATGCTCCA TATTTATTTT TACAAAAGCA GTATGACCAA 
CTCAGTCTCT CTACTAAACT CATAGCAAGT CTTGGGTCAA ACACTGCAAT GGCTTACGGG 
TTCCAGTTAA TGTTAATGTA TGAGGGTACC AGTGAAGGAA TACAATGGTC CAATATAGGA 
AGACCCAATA CTCCCGATGA CAGTCTAACG CTCGGTTACA TAATGATGAT GCTGGTAATA 
GACACTTTTA TCTATCTTTT TGTGGCTCTT TACGTCGAAG CCGTTTTTCC CGGGGAGTAC 
GGAGTGCCAC TTCCTTGGTA CTTCCCGTTT ACTTCCGCCT ATTGGTGTGG TCATCCTAGC 
TATGTAGGAG TTGAAGATTT CACTAGTGTC CCATCACAAG AAGGTGAATT TTTTGAAAAA 
GAACCTAACA ATCTTACACC CGGTATACAA ATTCGAAATT TGCGGAAAGT TTTTGACCAA 
AAAGCAGCAG TTCGAAATCT CTCGCTAAAC ATGTACAACG ATCAAATTAC GGTCCTTCTT 
GGACACAATG GTGCCGGAAA AACTACCACC ATGTCCATGC TGACTGGAAT GTTTCCTCCC 
ACAAGTGGTA CTGCAGTCGT TTGTGGACAC GACATTAAAA CGGATATGGA CGGAGTAAGA 
GAAAGTCTGG GTCTATGTCC TCAACATAAC ATCATCTTCG ACGAACTCAC CGTCCGAGAG 
CATCTCTACT TTTTCAGTAA ACTTAAAGGA CTGAAACGTG AAGCAATCAA TGAAGAAATT 
GATAAATACG TCGAACTGTT GGAACTGCAA CCTAAAGCGA ATGCAAAATC GGCCACTTTG 
TCAGGAGGAA TGAAGAGAAA ACTTTGTGTG GGTATGGCAT TGTGTGGCAA CTCTAAAGTT 
GTCATGCTTG ATGAACCCAC TGCCGGAATG GATCCATCAG CAAGACGAGC TTTGTGGGAT 
TTGCTTCAAA ATCAAAAACA CGGAAGGACG ATGCTTTTAA CAACACATTT TATGGACGAA 
GCTGATTTAT TAGGTGATAG AATCGCTATT ATGGCCGGAG GGGAGTTACA GTGTTGCGGC 
TCAAGTTTCT TCCTAAAGAA GAAATACGGG ACGGGATACC ATCTCATAAT GGATAAGGCT 
TCGAATTGTC AAGAGGAACA AGTAACACAA CTTTTGAAGC GACATATTCC TAATATCCAG 
GTACACGGTA ATGTTGGCTC TGAACTCACG TATCTACTAT CCGAAGATCA CTCTTCAGTT 
TTTGAAGAAA TGCTGCGAGA CTTAGAAGAA AATAGTGATA GTTTGGGTAT TCGTAGTTAC 
GGAATTTCAC TAACAACTTT AGAAGAAGTT TTCATGAAAG TCGGTGCTGA CCACGGCCAG 
GAAGAAAGCT ATAACGGTTT TGTGCAAGAA ACAGATTGTA ATAAAACAAT GCTGGGTAAA 
ATAAAATCGA AATTGAAAAG GAATGAAAAC TACGAAAATA GCATTAACGA AACAACTTTA 
AACCTTGAAA CTTCTGCTCT TCTAACCGGT TTCATGTTAA TCAAAAATCA GTTTTTGGCA 
ATGCTTATGA AAAAAATTTT ATCAATCGTA CGAACCTGGA TATTGCAGTT TATTCAAATT 
TTGATGCCAG TTGCGTTTCT TATCATAGCC ATTGTCGTTA GCAGAAACAC TAACAAATCA 
GCAGATTTAC CCAAACTGCC CATAACATTA GATTCGTATC GCAATCCAGT AACGCTAATT 
GAAGATAATG CAAATAACTC ATATTCGGCC TTGTATATGC AAAGCCTTAA AGGTTATGAA 
ATTCAAAGAG TGGCAAGCAT CCACGATGTA ATGCTTAATT TGACCGTAAC TGTTCCATCC 
ACTGTCAGAC TAAGATACAT TGTTGGAGCT AGTTTCAACA AAACATTCGG CGGTGTTTTT 
ACAACTATCA CAGCCTGGTT TAATAATAAC CCTTACCACT CCCCGCCTCT AGCATTAGGT 
CTTGCCTTAA ACGTGGCTTT CAAGCATTTA GCTGGCACAA ATCGTTCAAT AAGATGGGCA 
AATTATCCGT TACCGTTTAC AGCCGATACA AAAGTGCAGC AACTGTTGGA AGGCAACAGT 
ATGGGTTTCC AGCTCGCTTT CAACATCGGA TTTAGTATGG CTTTTGTATC CTCATTCTAC 
GTCCTATTTT ATGTGCGGGA ACGAGTTTGC AAGTCAAAAC ATTTACAATT CGTGTCCGGA 
GTGAAAGTTT ACATATTCTG GATAATGTCA TTCGTCTGTG ATATAATCAC GTTCATTGTG 
ACCATCATCG CGATCATCAT TACTTTGGCG TGTTTCCAAG AAGATGGCTT CCGGTCGGCT 
GAGGACTTAG GTCGCATGTT CCTTCTTTTG TTCTATTTTG GCGTTGCAAT GTTGCCTATG 
ATGTACTTTG CGTCATACTT GTTCGAAGTA CCTTCGACAG GTTATACTAG AATGACCCTC 
TTTAGTGTTT TCACTGGAGT GGCTGCCTTT TTGGTTGTTC AAGTTTTGGC AACACCTGGA 
TTGGATTTGG AATATGTCGC GAATACTCTA CATTGGATTT TCCTGGTAGT ACCCCATTAT 
TCGTTAGCTA CAGGTATTAG AGACGTTTAT ACAACATTTG CCACTAATAA AATGTGCAAA 
TACGCCGTCC AAACGTGCGT CGAGAATAGG CCAGATATGA CTGTGGACAA GTGTTGGGAT 
TTGACTTGTC ATAATGCAAG TGCTGTGCTA CAGGATTACT GTTGTGTACG CGAGCCGCAC 
TATTACAAAT GGGCCAGTCC TGGAATAGGC CGAAATTTGC TATACTCATT TTCCGTTGGA 
ATAGTCCTAT TTATTCTACT CAACATAAAA GAATATAAAA TATTTTCAAA AACGATAGAC 
CATATTGTTG AGAGCTATAG AAACAAATTG CCCCAGCCAG TGGAAAATGA AGACAGCGAT 
GTCGCGGAAG AAAAACACAA AATCCACAAC ACTTCTGAAA CAGAGTTACA AAAAGACTAC 
ATTTTGGTGC TTAAAGATTT GACAAAGTAT TACAACAACT TTTTGGCAGT TAACGGATTG 
TGTCTGGGTG TTAAGGAACG TGAATGCTTC GGATTATTGG GTATAAACGG TGCTGGGAAA 
ACCACAACTT TTAAAATGAT GACCGGTGAT GTAAAAATCA CGCACGGAGA AGCTTGGGTG 
AAAAAATTCA GTATCAAATC GCAACTAAAA CAAGTGCAAA AGTTGATTGG ATATTGTCCG 
CAATTTGACG CTCTTCTTGA TGACCTGACT GCCAAAGAAT CATTGGTAAT GTTTGCACTT 
TTGAGAGGAG TTCCAATGGA CGAGTGCCGA TACCTGGTCC AGAAACTATC GACAGATTTT 
GATTTCCAAA GACATTTGAA TAAAAAGGTT AAGGAACTTA GTGGTGGTAA CAAGAGGAAG 
TTAAGTACTG CCATTTCGCT GATTGGCGAT CCTCCGGTGA TATATTTAGA CGAACCTACG 
ACAGGAATGG ACCCTGCGAC GAAACGCTAT TTGTGGGATG CCATCTGCAA AGTTAGAGAT 
AACGGAAAAT GTGTAGTCTT GACCTCTCAT AGTATGGAAG AATGTGAAGC TTTGTGCACA 
AGACTAGCCA TAATGGTTAA TGGCAATTTC AAATGTTTGG GTTCCACTCA ACATTTGAAA 
AATAAATTTG CCGAAGGGTA TACGTTGACA ATTAAAATTA AGAAAAGCCC TGGCGGGGAA 
GATGCCGATA TTAAACCAAT TGAAAATTTC ATCTCGGACC ATTTTCCCAA AGCAATACAA 
AGAGAAAAAC ATCAAGAGCT TTTAACATAT TATATTGTAG ATAAAAGCAT CCCCTGGTCC 
AGAATGTTCG GGATTTTGGA AAAAGGAAAA AAGCAGCTAA ATATTGAAGA TTATTCTTTG 
GGTCAATCAA GTTTGGAACA GGTATTTTTA TCTTTCACAA GGTTGCAACG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC659344 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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