Rapid assembly of gene variant libraries to engineer proteins for user defined goals

Mutant libraries can be used to optimize protein function, such as enhancing expression levels, protein stability and activity, antibody affinity maturation and removal of hot spot degradation. Traditional methods for creating these libraries suffer from limited control over the codons introduced and variants can incur significant codon bias and early termination. The result is a large library with poor representation of variants causing a significant screening effort on the part of the researcher to ensure they are capturing the entire variation of the library. In this webinar, we will discuss a novel method for creating mutant libraries using a semiconductor-based oligonucleotide synthesis platform.

Speaker Bio

Charlie Zou, Ph.D.

Project Manager, GenScript

Charlie Zou received his B.S. degree in Biotechnology at Nanjing Normal University, M.S. degree in Biology at St Mary’s University and completed his Ph.D. in Microbiology & Immunology at Saskatchewan University. He is currently a project manager in the R&D department at GenScript. His previous research focused on studying the Neisseria gonorrhoeae cell division interactome and rhizobacteria of legume crops and mechanisms of rotation benefit which resulted in numerous publications.

Related Services

Speaker-related Webinars

Identify the optimal protein purification strategy for your recombinant protein production

Are you challenged with obtaining sufficient amount of high purity proteins for your applications? Almost unavoidably, every protein purification step results in some degree of protein loss.

Learn More

Recombinant protein expression & purification

Are you struggling with the amount and/or purity of your recombinant protein? Attend this online seminar for solutions to the challenges in recombinant protein expression & purification.

Learn More

Accelerating Lead Identification Through Paramagnetic Bead Purification Technology

High throughput purification is a critical step in antibody engineering and lead identification. Paramagnetic bead technology allows a single step purification of targets by simply adding the beads directly

Learn More

Efficient stable cell pool/cell line development for large-scale antibody and protein production

Interested in rapid and efficient stable cell pool/cell line development for large-scale production of your recombinant antibodies and proteins? If so, this webinar may be for you.

Learn More

Chaperone co expression strategies for recombinant soluble protein production in E. Coli

Recombinant protein expression in E. coli often results in the formation of insoluble aggregates called inclusion bodies.

Learn More