DNA Mutant Library

Empowering Breakthroughs with Precision, Consistency, and Capacity

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Overview

Utilizing our advanced gene synthesis technology, including GenTitan™ semiconductor-based, trimer, and degenerative oligo synthesis techniques, we create highly customized mutant libraries with exceptional coverage. Our Precision Mutant Library service ensures 100% user-defined codon usage, unbiased variant distribution, and vast theoretical diversity, reaching up to billions of variants. GenScript's Precision Mutant Libraries offer the largest library sizes at competitive prices and industry-leading speeds, enhancing high-throughput screening and accelerating the discovery process.

Highlights

Industry-leading Speed
starting at 2 weeks

Precise Control
over Codon Usage and Amino
Acid Distribution

Guaranteed >95%
Coverage of all
Desired Variants

Industry Leading
Acceptance Rate

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Services

Site-Saturation Mutant Library

Mutate up to 20 amino acids at single sites, either consecutively or scattered throughout the ORF.

Precision

Complete control over codon usage (all 64 codons available)

No premature stop codons and unwanted codons

Superior Quality

High uniformity of mutant representation verified by NGS

Flexibility

One position per well in a plate or all positions pooled in a single tube

Deliverables in dsDNA fragments or cloned plasmids

Saturation Scanning Mutant Library

Mutate up to 20 amino acids at consecutive sites within a defined region of the sequence or the entire protein sequence.

Precision

Complete control over codon usage (all 64 codons available)

No premature stop codons and unwanted codons

Superior Quality

High uniformity of mutant representation verified by NGS

Flexibility

Hundreds of positions are acceptable, with all positions delivered as a single pool

Deliverables in dsDNA fragments or cloned plasmids

Combinatorial Mutant Library

Mutate multiple positions simultaneously to achieve high diversity across specific target regions of the ORF.

Precision

Complete flexibility in codon usage and oligo format

User-defined amino acid composition and distribution

Superior diversity coverage

Superior Quality

High coverage and uniformity mutant representation verified by NGS

Optional third-generation long-read sequencing available

Flexibility

Deliverables in dsDNA fragments or cloned plasmids

All mutants delivered as a single pool

CRISPR gRNA Library

Customized and Off-the-shelf knockout, activation and pathway-specific CRISPR gRNA library.

Precision

Millions of gRNAs synthesized using the GenTitan™ silicon-based platform

Superior Quality

High coverage and uniformity mutant representation verified by NGS

Top-notch Speed

Next-day delivery: Off-the-shelf GeCKO and SAM gRNA libraries

Customized library delivered starting from 2 weeks

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Applications

Antibody Discovery

Enzyme Optimization

CAR/TCR Engineering

AAV Engineering

Overview

DNA mutant libraries are used in antibody engineering to generate diverse antibody variants with targeted mutations. These libraries enable high-throughput screening to identify variants with enhanced binding affinity and stability, accelerating the optimization and development of therapeutic antibodies.

How can GenScript help you from real success stories

Two-Step DNA Mutant Library Strategy to Achieve a 1000-Affinity Boost

Through two rounds of optimization using GenScript’s DNA Mutant Library, significant improvements were achieved in the affinity maturation of a monoclonal antibody. Initially, a saturation scanning library was created with 63 amino acids and 19 non-wild type amino acids per position across 6 CDR regions. The top 5 candidates were selected for a second round of combinatorial optimization. The novel antibody from this mutant library showed a 1000-fold increase in binding affinity compared to the wildtype.

Two-step antibody affinity maturation workflow

SPR Ranking of wildtype versus identified top candidate

Variants	KD (M)	Rmax
Wildtype	5.39E-10	107.2
Top Candidate	1.23E-13	13.7

Resources

Combinatorial Mutant Library facilitates IgG antibody affinity maturation via phage display Free Download

Accelerating Antibody Engineering with DNA Mutant Libraries: A Two Rounds Strategy to Achieve 1000-Fold Affinity Boost Free Download

Accelerating Enzyme Optimization: Strategic Collaboration with GenScript and Allozymes Free Download

How to Order

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Our technical account manager will contact you with a quote within 24 hours

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Customer Testimonials

"I have ordered many products from GenScript (plasmids (more than 15), AAV2 products, and contracted them to produce mutants of plasmids. Their services are amazing and on time. I recommend GenScript for any colleague."

Adnan Dibas

NanoScope Technologies LLC

"I have been using GenScript Molecular Biology services for more than 15 years - always with great results and fastest turn-around times. Over the years, GenScript expanded its services while lowering prices for routine Molecular Biology tasks. What really impressed me with GenScript, it was their willingness to go one step further in order to offer additional validation and characterization data whenever we needed those. For example, GenScript performed optimization of transduction protocols with the viral vectors they were preparing for us, based on which we were able to better estimate our needs for the virus. In order to validate their viral vectors with our targeted cell lines, GenScript performed analysis of the target expression by flow cytometry and Western blot analyses practically free of charge, passing on us only the cost of reagents. All the deliverables and results of additional experiments performed by GenScript were of high quality and great value to us. "

Yuri Bukhtiyarov

Claim Therapeutics

"We have been working with GenScript on our projects for a few years. Their services are reliable, quick, and cheap. We have continuous exchanges with vendors and technical support for our projects. "

Maria Pennuto

Università di Padova

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DNA Mutant Library

Overview

Highlights

Services

Applications

Resources

How to Order