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Home » Peptide Services » Custom Peptide Synthesis » Recommended Peptide Purity Levels & Applications

Overview

Peptide purity, usually determined by reversed-phase HPLC, refers to the percentage of the target peptide compared to impurities that absorb at 220 nm (peptide bond absorption wavelength). Impurities are usually deletion peptide sequences generated during peptide synthesis. Peptide purity will affect experimental results since the lot-to-lot variability becomes even greater the lower the peptide purity is (especially < 80% purity).

  • >98% Purity

    UU8 IDE Ge-puntesty8icptioptty

  • >75% Purity

    UU5 IDE Ge-puntesty5icptioptty

Why higher purity peptide is necessary?

  • results icon

    Better Results

    Higher success rate in obtaining real results

  • Smooth Project Progress icon

    Smooth Project Progress

    Less unnecessary repeat caused by impurity

  • needed icon

    Most Needed

    90%+ scientists prefer peptides over >75% purity

Peptide Services

Recommended Peptide Purity Based on Application

GenScript offers reliable peptides with purities ranging from crude to as high as ≥98%, at 100% guaranteed quantity with fast delivery to support your peptide-related projects.

An appropriate peptide purity is a critical factor for the success of a scientific experiment. In general, the higher the peptide purity level, the more favorable the experimental outcome. Impurities in lower purity peptides may result in toxicity or unwanted side reactions, especially for some applications such as animal studies where exceedingly pure peptides (>98% purity) are required. Considering budget, there are minimum acceptable purity levels recommended for various applications as follows:

Minimum Purity Application
Crude
preferably higher purity
  • First pass non-sensitive screening
  • Mutation screening
  • Sequence optimization
  • Protein-protein interactions
  • Receptor-ligand interactions
Immuno grade purity
≥75%
  • ELISA testing
  • Peptide arrays
  • Antigens for polyclonal antibody production or affinity purification
Biochemistry Grade purity
≥85%
  • In-vitro bioassays
  • Epitope mapping
  • Polyclonal antibody production
  • Phosphorylation studies
  • Cell attachment studies
  • Protein electrophoresis applications
  • Semi-quantitative enzyme-substrate studies
  • Peptide blocking studies for Western Blot (non-quantitative)
  • Coupling to chromatography resins for affinity purifications
High Purity Grade Peptide purity
≥95%
  • In vitro bioassays
  • In vitro studies
  • NMR studies
  • Quantitative receptor-ligand interactions studies
  • Quantitative blocking and competitive inhibition studies
  • Quantitative phosphorylation studies
  • Quantitative proteolysis studies
Industrial Grade purity
≥98%
  • Crystallography
  • GMP peptides for drug studies
  • Cosmetic peptides for cosmeceuticals industry
  • Clinical trials
  • SAR studies

Still not sure which purity to select? Contact our experienced technical account managers at peptide@genscript.com to decide.

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