Oligo Pool Application Notes

CRISPR gRNA libraries provide a great tool for genome-wide and gene-focused applications, such as studying molecular targets or therapeutic target screening. However, to have a successful CRISPR gRNA library, the quality of the gRNAs is important and requires ultimate control over the synthesis process. This application note will demonstrate how using an arrayed, semiconductor-based oligonucleotide synthesis platform for synthesis of gRNAs creates a reliable, pooled CRISPR gRNA library for rapid high-throughput screening of molecular targets.

The key to excellent target enrichment relies on the use of high quality capture probes and creating optimized target enrichment probes improves the efficiency of targeted NGS and reduces the number of sequencing reads needed to obtain high-confidence data. In this application note, learn how array-synthesized, double stranded DNA capture probes can provide improved and consistent target capture in NGS targeted sequencing.

Mutant libraries are used to identify critical residues within a protein or to optimize protein function. Traditional methods for mutant library construction, such as using error-prone PCR or degenerate oligos, introduce mutations across the sequence space, however, have limited control over the codons introduced and variants can incur significant codon bias. The result is a large library with poor variant representation and a large screening burden to capture the entire variation of the library.