GenXceed Cell-free mRNA Template

• Step 1: Optimized DNA Input for RCA → Proprietary strains and vectors ensure high Poly(A) integrity and sequence stability, optimizing RCA amplification efficiency.
• Step 2: RCA Amplification → Plasmid-Free, exponentially scalable DNA synthesis with zero bacterial contaminants.
• Step 3: Linearization → 99.9%+ DNA purity, 100% plasmid yield, and zero host-cell contaminants.
• Step 4: DNA Purification → Streamlined process, ensures ultra-low endotoxin & IVT compatibility.
• Step 5: Final Purification & QC → Poly(A) integrity verified, with high uniformity.
• Step 6: IVT-Ready DNA → Optimized for Seamless mRNA Synthesis, No Extra Steps Needed.

Competitive Advantages

Why GenXceed™?

RCA-Powered Speed Redefines Industry Standards

High-yield DNA synthesis in just 8 BDs, no large-scale plasmid expansion.

Poly(A) Guaranteed

Verified integrity for peak IVT efficiency—no uncertainties.

Purity Beyond Limits

Minimal host DNA, ultra-low endotoxin, and enzymatic precision for flawless templates.

Next-Gen Disruption

No plasmid baggage, no fermentation—just scalable, high-yield DNA redefined.

Unmatched Purity – Optimized for High-Performance IVT

GenXceed™ delivers ultra-pure, IVT-ready DNA with stringent quality control, ensuring optimal performance in mRNA synthesis. As shown below, our templates exhibit:

• Exceptional DNA Purity – Verified by AGE and IEC-HPLC analysis, achieving >98% purity, minimizing unwanted contaminants.
• Ultra-Low Endotoxin & Contaminants – Ensuring a clean template for high-efficiency IVT.
• Poly(A) Integrity Verified – Sanger sequencing confirms a complete and stable Poly(A) tail, essential for translation efficiency and mRNA stability.

Fig 1. DNA Template Purity by AGE

Fig 2. DNA Template Purity by IEC-HPLC

Fig 3. PolyA Uniformity by FA5200 Analyzer

Fig 4. Sanger Sequencing for Poly(A) Validation

Consistent IVT Performance

GenXceed™ mRNA templates deliver unmatched IVT performance—exceptional purity, minimal contaminants, and consistently high expression levels. Engineered for efficiency, our RCA-powered DNA templates ensure seamless translation into reliable, high-quality mRNA, driving superior downstream results.

• IVT mRNA Purity by AGE - Gel electrophoresis confirms the high integrity of IVT mRNA, with no visible degradation or unwanted byproducts.
• Ultra-Low Endotoxin & Contaminants – Ensuring a clean template for high-efficiency IVT.
• Poly(A) Integrity Verified – Sanger sequencing confirms a complete and stable Poly(A) tail, essential for translation efficiency and mRNA stability.

FAQ

• What makes GenXceed™ Cell-Free mRNA Template different from traditional plasmid-based DNA production?

  GenXceed™ utilizes Rolling Circle Amplification (RCA), a cell-free, enzymatic amplification process that eliminates the need for large-scale bacterial culture and plasmid extraction. This results in:

  • Higher purity – No bacterial host DNA contamination
  • Minimal endotoxin levels – Avoids endotoxin accumulation from bacterial fermentation
  • Faster turnaround – As fast as 8 business days, compared to 17-22 days for plasmid-based methods
• How does GenXceed™ ensure high purity and IVT compatibility?

  GenXceed™ templates undergo rigorous multi-step purification and quality control, ensuring:

  • Endotoxin levels <0.05 EU/µg – Minimizing immune response risks in downstream applications
  • No host DNA carryover – Eliminating bacterial genome contamination
  • Pre-validated IVT compatibility – Delivering high-yield, clean transcription
• How does GenXceed™ linear DNA compare to traditional linearized plasmid DNA?

  GenXceed™ RCA-based linear DNA offers superior IVT performance while eliminating key drawbacks of plasmid-derived templates:

  • Pre-validated Poly(A) integrity – Ensuring stable and efficient mRNA synthesis
• Can GenXceed™ support customized mRNA template designs?

  Absolutely. Our platform offers flexible design options, including:

  • Custom Poly(A) Tail Lengths – Tailored to enhance IVT efficiency for specific applications
  • Vector Optimization – Ensuring the highest yield and stability for mRNA synthesis
  • Sequence Modifications – Supporting specialized research and therapeutic needs