LOC108699444 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108699444
Entrez Gene ID	108699444
Full Name	E3 ubiquitin-protein ligase RNF213-like
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018231527.1	XP_018087016.1	E3 ubiquitin-protein ligase RNF213-like isoform X1
XM_018231528.1	XP_018087017.1	E3 ubiquitin-protein ligase RNF213-like isoform X2
XM_018231529.1	XP_018087018.1	E3 ubiquitin-protein ligase RNF213-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC108699444 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108699444 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAf43052	XM_018231527.1 Latest version!	Xenopus laevis E3 ubiquitin-protein ligase RNF213-like (LOC108699444), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAf43053	XM_018231528.1 Latest version!	Xenopus laevis E3 ubiquitin-protein ligase RNF213-like (LOC108699444), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAf43054	XM_018231529.1 Latest version!	Xenopus laevis E3 ubiquitin-protein ligase RNF213-like (LOC108699444), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108699444 ( XM_018231527.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018087016.1 Xenopus laevis E3 ubiquitin-protein ligase RNF213-like (LOC108699444), transcript variant X1, mRNA.

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CloneID	OAf43052
Clone ID Related Accession (Same CDS sequence)	XM_018231527.1
Accession Version	XM_018231527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase RNF213-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGTTT GTTTGTTTTT CTTAAAACAG TTGATTAATT TAATAATGGG AAAACTCTAC 
CAACATCTTA TCAACAAGAA TTTATCAGCA GTGGATGCCA TTATAAAAGA CAGGCTGATG 
GCTGGATTAC TCTGCGCCAA ACTGATAGAA ACCTACTCAC TTCCTGCTGA CAAGGATTCT 
CTTGTAAAGG TTTGCAGACT CCTCTCTTTG GAGCAGATGC TTAACAAAAA CCTACCCATC 
CAACTTGAGG AACTGAAAAG AATCTTCTCA GTGTACGAGG GAATAGAGTA TTCCCTGAAA 
CTATTCTGCA TGAGATGCAT TGATGGTAAA ATTGAAGACT GGGTGCAGAT ATTGCCTGTT 
TATCACACAT TTAACACTCC GGCTAAACCA GAGGATTCCA CACAACTAAA ACCAGAGGAA 
AACTGGGCAG GTTTGGACGG GCTGCCATAT CAAACCATTG TGATACGGTC TAAGTTTTAC 
AATAGAGATG TCCAAAAATA CATGAGGAGT AAGGAGAGTT TACAACAGGC AGGCAGAACT 
TTACACAGAT CTTGGCTGTG TCTATTACCA CTGGAAAACT TGTTCACGTT TTTGGATACA 
ATTCCCACCG ATTTCCGAGA TGCTTTTACC GCTGCCTGGT ACAAATTTCA TACACAATCC 
TCAAGCAGCA AAGATAAGCA CATTGAGAAG ATTCTAACAA AACTTCTGAA TATGGCAAAA 
GGACCGAGCC AGGGGTCACT GCAGAAGGAC TCCATATACC TGTGTAATAT CAGTTTGAAA 
CTTCATCATT TAATCTGCAC TCAAACAGGG CTGAATAAAA ACAGTCAGCT CCCACTTTTG 
TCTTTCAAGA TCGCACTAAA GCTTTTTGGA GCAGCACAGA AACAGGAGCC AATGAAAGGG 
AGGGAGACCA GTATCAATGA AGTGCAAAGC AATTTAAACA AAGCTGTTGA GATGCTGAAG 
ACATGGCTAC AGGACTTCTT TGGACATAGA TTTAATTTTA GACGCTATGG ATATCCAACA 
TTTGGGGATG AACTTGAGTT TTGGAATAAC CTTCTTATGA ATGACGACAA TATGGACCTG 
AAGTGGAAAG AACTGCTTCT CTCAGATCTG GCCAGGAAAA TAAAAGAGGA AAGCCCTGTG 
GAACAGATTA AAATCTTTTG CAGAGAACAT TCACTGTTGC AACGGCTTCA TATCTCCATA 
GAAGAATGCT TTGAGGAATG CGCCATTAAT GCTGTTCATA ATGCATATAA GTCTCATAAC 
AATATTCTCA ACCAAATCTC AATAAACAAT CTACAACATT TTAGAAAACT GGTATCAACA 
ATAATAAGAA ACTCTTGGCC AAAGGATGTG CAGGGAAATC CAACTGAGAA TAATAATGAG 
ATTTTAAAGC ACATGCTGCA CTGGAAGGAT TTCACTCATA TATTCAAACT ATATGATCCT 
AACCAACCAA TCACCTCTCA GTTTATAGAA GAAACTCAAA AGCTTGTTAC TTTGTCAGAT 
TCGGTCCTTC TTGACACCGT TTGTCGCCTA ATGCATGGAG ATATTCTGTA TGAACACCTC 
AAGTTGGTAC TGGGCAATAA AAACCAGTTC TTGGATATAT GTAGACTGAG AGAAACCATA 
AAGGTGGAAG ATGTTAAGAA AGTTCTGGAG TGGAGGAATG AAGAGCTTAA AACAGTGAAC 
TGTAAGAAAA CATGGGTGGA GAACTTGCTG AAAACGTGCC AGAACATCAG CAAGTCTGTC 
CGAGTGAATA CTGAGGAAAT TCAACAAATC CTTTCTGTAA ATCTGGGAGC ACAAAAGTTA 
AATGATTTAT TACCTGTTAA GACACTTGGT GCTACTGAAC CTCCTGAAGA CAAGGTGACA 
TTGTTTAACA GTTTCACTAA TTCAATAATG TCAATGGCCG AGAGTCTTCA CACCCACCGG 
CACAGTCATG TTTTCCGCAT GTTGTGGAAT GAGGCTGTTA GCTCTCTTTC AAAGAGAGAT 
GAAAATTTAG AAAATGAAGA AGATGATGAA CTTACTTTGG ATGAGATGCA GGAATCCTTG 
TTCACACCAT GTTTTGATAA ATACACCAAG ATATACGAGG ACATCAGATC TGAAGAAGTG 
ACCTTTGAAG TCCTGGACAC ATATTTCAAG ACTTTCAAAA ACCATTACCA AGCCCTACAT 
AAAGAACTAC AAACCATACG CCAGTTAGAT CCAGAGGATA ATGGTTCCTG GGTCAGTGGC 
CGAATAAGGA AAATTGAGCA GTATCATCAG TTACGTGAAG CCTTTGAATC AGCCCAGATT 
GTTGATGACA TCAGAGATTT CTGTAAACTT TTGGGAGATT TTAAGACCCT GAATACTCTG 
CAACAATTTG GTGCTGACTT TAGTGATTAC AAGGAAAAAC CTTTATCTTT TATCACTGAA 
GACATTGTAA AGACAAAGGA AGCGCTTTGT GAGATAACAC AAGAACGTGC TGAGGCTCTG 
AAGGAGGCTT TAATGAGAAA GGAATTTATA AAGTGGGTTC GAAACTCACT GGAAGATGTC 
AATGAACTGA AAGTTTTTGT GGATCTGGCC TCCATTTCGG CTGGTGAGAA TGACATGGAT 
GTTGACCGTG TTGCTCAGTT CCATGATGCT GTTTTGGGCT ACGCCCCTAT TTTGTATGAA 
CTCAAAGAGG AATTTGGATT TAATGAATTA ATGTTATGCT TGAAAAAACT TTGGAAAGCC 
CTGGAAAGTG ACCCAAATCT GCAGAAAAAA CTGAGAGACT CTGCCCGTCA CATAGAGTGG 
CTGAAAACTG TAAAAGAAAG TCATGGATCT GTGGAACTCT CATCACTGTC TCTGGCATCT 
GTTATAAACC AAAGAGGCAT CTACATTATT CAAGAGCCCA ATGAGAACCT AAAGATAAAC 
CCAGACAGTG TCCTGAGACT AGAAATTCCT GATGATGATC TGGAACAGAA CCAGGTGCGC 
ACCTTTAATT TAGAAGAACT GAAGGAGCTG TTGAGCAAGC TCATGCTTAT GTCTGGGAAA 
GGTGACCAGG GTAATGCTGA AGTTGAAAAG TTTTCAGAGA TTTTCTCCAA TGTTCAGAGA 
CTTGCCAAGG CCTACATTGA TCTCTGCTTG TCTGGAGACA TTTTATTCAA AACTTGGGAG 
GCCACTATCT ACTGCTCAGA TGAGATTGAT ATTGCTATTA GGATGAAATT TAAAGTAACA 
GTAACAGCCA TTGGGGAGAT AGGAGCAACA GGAAATATTT TTGAACTGCT TCCCAAAATC 
TGCCAAACCA TGGAAGACAT ATTAGAAAAA AGGCAAACTT TTATGGATGA AAAACGTTCT 
CAGCATTACT TTCTAAATTA CTACACCGCA GAGCAGTTGG TCTTCCTTAG CCAGCAGATT 
CAGAAGCCTG ATGTTAGTGA AGAGCTGCTT GTTATGTTAT CCTTTATCAA GCCTGAATGT 
ACAAAATCTG ACACCCAGCA AGCTTTATTA GCTGCACGGA GGAAAGGAAC ATCAAAGCTC 
CTTGCGACTA CTAATAATTC TGCTTTGTTT AACTTTAAAA AATCTACCAA TGTTCAAGAG 
AAACTACAGA TTGCTTGGAA ATACTCCATG GACTGCATGA ACAATTTGTT TCCTGGATGT 
CTGGATATAA ATACACTTGG AAGGTCCTTA ACTGAACTGG CAAAAAAGGA AAATAGCCCA 
GTGACCAGAA GTCTACATCC CAGTCTAAAA CCGGGATGTC CCAATTTGGT ACTCTGTGCA 
TCATCAGAGA TTCTATCTTC AGCTCTTGCT GTATATATGC AGAATTTAAA TCAGCCACTT 
CCATCTTATG ATGAAGTTCT TCTCTGTTCA CCTAAAACTA CTTACGAGGA GGTGGCATTG 
TTTTTGAGAA GATGTTTCAC ACCAGGCTAC AGTGGGAAGA AGATTTACAG TTTGCTGTAT 
GCAGAAGATC TCAGCTATGA TACAGCCTAT AGAGCTGAAC AACTGTTTTA CCAACTGCAA 
AAGACAAAAG GCCTAGACTA CCACCTTGTG ATTATCTGTG ACCGTGAGAG ACATCATTTG 
CATATACCAT CTACCTTTAG CCAATACAAA GTGCAAGTTG TTCCTCAGAG ACTTTTTGTT 
GAAGTTCAAA AATATTTATT GCAACATTTT ACTATTGATC CAAATGTAGT CTCAGCTGCC 
TCCGTTTTTA AAGGCGTGAG TGTGGGAATA GTGACCTCCA AACGAGCAGG AGTTGGAAAG 
TCGCTCTATG TGAAGAGACT ATATGAAAGG CTGAAACTGG AATTTCCAAA TGAAAAGCCT 
CTCCAAAAAA TAATCCGAAT GTTTCAGCCC CAGGTGGACG AAAACAAAGT TCTGCAAAAG 
CTTCTTCCAT TTCTAACCAA GGAAAATAAA AATGTGCCCA TAATATTTCA TATTGATATC 
ACCTCTTCTG TTAACAGGGG AATTTCAGAA TTTCTTTTCA AACTTTTGAT TCTTCAATAT 
CTAAAAGATG ATGAAGGGAG AATGTGGAAG CGCCAGTCTT GTCAGCTTTA CCTTATTGAA 
ATCTTAGAAT CTTCTTGCAT AATTTCTGAG AAGCAGAAAA AACCTGCACC TCAGATGGCG 
CAAAATATTT TTATGGACTT TTTCCCTAAA ATATCATGCT GTTCTCCAAA TGAGGTGCTG 
ACAAAAGTCA CAGAAAATCA GACTGGACAA AATGAAGATC CTGGAATGGA TAGAAAGGAA 
TTTGAGAGTG AATGCTTTCA GCGGCCTTTT CAGTACTTGA TACGTTTTGA AGGGAAACAA 
AGTCTGGATA AATTTACATA CAAGGAAGGT TCCGTAGAAG GAAATGAGGC AAAATGTCTG 
CAGTTGTTCC TTTTGCACTG TGGGGTTATG GACCCATCAT GGGCAGAACT TCGAAATTTT 
GCATGGTTCT TAAATGTTCA GTTAAAAGAC AGTGAGAATT CTGTTTTCTG CAAACCAGAA 
TTTGTGGAAG ATGCTATGCA GGGCTTCAAG AATTTTGTTG TAGACTTTAT GATATTGATG 
GCAAAAGATT TTGCAACTCC TTCATTACAG ATCACTGATG AGAGTCCAGG CAGACAAACT 
TGTGAAGAAG TCTGTACTAA TGATGGAGAC CTTGCTCCAT TTCAGATACG TAAAAAATGG 
GAGGCTGAGC CACATCCCTA CATTTTCTTC AATGATGATC GCATGACAAT AACATTTATC 
GGCTTTCACT TGTGCGTGAA CAGTGCTGGA AATGTTGATG CGATCAATCC TAGAGATAAG 
AGCATTATTA AGAAAAATAT TATGACAAGA CAACTTTATC GAGACTTAAT GCTTCAGAAA 
GTTCAGTTCG ATATAGACTT TGACACTCTA CCTAGGGAGG AAAAGATTAA TATGTTATCC 
AGAGTTTTGG GGATTAATAA ACCGCAGGAT CCAGATGAAA CATATGAGTT GACTATGGAT 
AACACTCTGA AGATCCTGGC TATTACAATG AGGTTCCGGT GTGGAATTCC TGTTGTCATT 
ATGGGAGAAA CAGGTTGTGG AAAAACAAGA CTCATAAAGT TTATTTGTAA ATTATGGAAA 
GGAAACCTGA ATGCAGAAAA TACTACATCT GATGCAGAAA ATATGAAACT TGTAAAGGTT 
CATGGCGGTA CAAGTGCTGA GAGTATTAAC AAAAATGTGC TGGAGACACA AGCCATTGCC 
CTCACTAACA AACAGAACAA ATGTGATACA GTGTTGTTTT TTGATGAAGC AAACACAACT 
CAAGCCATAA GCAGTATCAA AGAAGTCCTG TGTGACCACA CTGTGGATGG CTTGCCTTTA 
ATTGAAAACT CAGGTTTGCA AGTCATTGCT GCATGTAACC CCTACAGGAG GCATACAGAA 
GAAATGATCA GACGATTGGA ATCTGCTGGT TTAGGGTACA GAGTAAAAGC AGATGAAACT 
AATGAACGAC TTGGGTCCAT CCCATTAAGA CAATTAGTCT ATCGTGTTCA TCCATTGCCT 
CCAAGTATAA TGCCACTGGT TTGGGATTTT GGTCAACTAA ATAATGAAAC CGAAAAGAAA 
TACATTCAAC AGATAGTACA GCATTTGGCA AATGAGATTG AAATGCATCA GAGCGATACA 
CAGATATTGA CCAATGTGCT TTCAGCTTCC CAGTCATATA TGAGAAATAG AAATGACGAA 
TGCAGCTTTG TAAGCCTTCG AGATGTTGAA CGTTGTGTAG AAGTGTTCAG GTGGTTCTAC 
CGCAATAGTG ACCTCTTGCA GGCAAATATA GAAAATAAAA AGGCGTGGTC TCTTGTTTTG 
GCCATTGGGG TATGTTACCA TGCCTCATTA GAAAAGAAAG ATGCATACAG AAAGGCAATT 
TGCCAATATT TTCCTGAACC CTACAGAAAC CAAAGAATAA TTCTTGAGAA AATAACAAGT 
ATCCAAGACC TCTTTCTAGA TCGTATACAA ATAAGAGACA ACATTGCCAG GAATCTTGCT 
TTGAAGGAAA ATCTATTCAT GATGGTGATT TGCATTGAGC TCAGAATTCC ATTATTTCTT 
GTTGGAAAGC CTGGAAGCTC AAAATCCCTT TCAAAGACCA TTGTTGCCGA TGCAATGCAA 
GGCCAAGCAT CACACACAGA ACTCTACAAA CATCTTAAGC AAATACACCT TGTATCTTTT 
CAGTGTAGTC CTCACTCAAC TCCAGAAGGC ATCATTGGCA CTTTCAGGCA CTGTGCTCGC 
TTCCAGAAAG ACAAGAACTT AAATGAGTAT GCTTCTGTGG TAGTGCTAGA TGAAATAGGG 
CTGGCAGAAG ACTCTCCTAA AATGCCTTTA AAAACATTGC ACCCTTTGCT GGAGGATGGT 
TGTATAGATG ATGAGACATC ACCTTACAAA AAAGTTGGCT TCATAGGAAT TTCTAACTGG 
GCGCTAGATC CTGCAAAAAT GAATAGAGGC TTATTTGTAT CCCGTGGAGA CCCTGATCAA 
AATGAGCTTA TTGAAAGTGC AAAGGGAATC TGCTCGTCTG ATCTCTTGAC CATTAAGGAA 
ATTGACCATC ATTTCAAAAG TTTTTCCGAT GCTTATCTGA AACTGTGTGA AAAACAAGAT 
AAAGAATTCT TTGGTTTACG AGATTTTTAC AGTTTGATAA AAATGGTGTT TACCTATGCG 
AAACACTTAA AAAATGAGCC AACACAGCTT GAGATTGCAA CAGCAATACT GAGGAATTTC 
AGTGGGAAGG ATGACTTGGA TGCCTTGAGT ATTTTTATAA CAAATCAAGA AGCAAACACA 
TATAGGGAGG AAATTAAAAT AATGGATTTG GTAATGGAGA ACATAAGAAA TGAGAGTGCT 
GATATAGAGG CTCGATACCT GTTGTTATTA ACTAGAAATT ACGCAGCACT GCAGATTCTA 
CAACAGACAC TGGCTAAAGA AAATCAACAT CCTGAGATTA TATTTGGGTC CAGCTTTCCA 
AAAGACCAAG AATATACTCA GATCTGCAGA AACATCAACC GTGTTAAAAT CTGTATGGAA 
ACAGGACAGA TGGTTGTCCT TCTTAATCTC CAAAATCTTT ATGAGAGCCT CTATGATGCA 
TTAAATCAAT ATTATGTCTA TCTAGCTGGA CAAAAATATG TTGACTTGGG TTTAGGCACA 
CATCGGGTAA AATGTAGAGT CCACCCAAAG TTTAGACTGA TAGTCATTGA AGAGAAAGAA 
ATTGTGTACA GAGATTTTCC AATTCCTCTT ATTAACAGAC TTGAGAAACA TTATCTGGAT 
CTAAACACAG TTCTAGAGAG GGGACAAAGA GCCGCTGTAG AGGAATTGAA TGACTGGGTT 
AAAGATTTCA CTGCAGGCGA CAGAGAACAA CTAATTGGCC AGAAACAGGA CTATAAACCT 
GCAGATGTCC TTATTGGTTA CCATTCTGAC ACCTGTGCAT CAGTGTTGTT ACAAATAACA 
GGAAAACTGA AAGAGGAATG TGGTGACAGT GACCTTTACA TGGCAGAAGT GAAAGAGGAG 
GCAAAGGCTA CTCTGCTGAA CTGTGCAACA CCCGATGCTG TCATTCGACT GGGCAGAAAA 
GAGTTGGTCG ATGAGTACTT CAACAAACAG AAACATGGAT CTCTGGTTGA CTTTGTTAAG 
AGCCACATCC ATTCAGGATC TAAACATCAA ATTGCATTTA CAGAGATCAC TACATTTTCA 
CGCCTTTTGA TGTCTATTGA CAAAAGAATT TTGGAAGCCG AGACAAAGGA TGATGTCAAA 
AGTGTAGAGG TGCTGTCATT GCAACAGTTT GATACGGAAC ATTCTTTCCT GAAAAAAATC 
AGGAGTTTTC TAAAAGATTC CACTGGTAAT AAAATACTGA TCATACAGTC AGACTTTGAA 
GATGGATCTC AAGGAGCCCA TTTGGTTGCA TCTGCCAAGT ACGCCACTGT AAATGAAATA 
AACAACTTAA AGAAAGACAA CACTGAGAAG ACATCAACCT TTGTCTATTT TATAACTAAA 
CTTCCTAGAA TACAAGGGGG GTCATCATAT GTGGGATTCC ATGGAGGACT TTGGCAATCT 
GTTCATATTG ATGACTTAAA GGAACCAAGC GATAAGTTGC CAGATGTAAC TGCCCTTCAA 
AACATGACAA TAAGCCAATT ATTTTATGGA GCCACAGCTG AATCACAGTT AAGATATGTT 
GATGGCATGG AAGGTGGCAC AGACGCTAGT TATGACACTG AGCCAGACCA GATACCAGAG 
GTAGTAAACA CCACCTTTCT CATTCGGAGC TGCATACAGA GTGCAGTGGG CATGATTACA 
GATGAAGAGA ATACTCCCAG CCGCAGCACA AGGAGGATTG AAATTCTAGT TAATCTACTG 
AGCACCGAGG ACAATCTAAG AGCTTCCCTT CTACAAACAC TGAAAGTTCG TGTTTATGAA 
ATTCTGAAGA ACCAGGAAGA GAATTCATAT AACCCAAATG AGTGGCTTGT AAGAGCTGCC 
TCCAACCTTG ATGCATTGCA AGAAGCTGGC ACATTCCGAT ACACTCTTTG GAAACGTATT 
CAGGCTGCTG TGATCCCAAT ATTTGCACAT CTCATTTATC TGATGGATTG TGATGCCAAC 
CTGGAGATTC TGGTGAACCC AGAAACTGAG GACTTTCTGA AATCTTTATG GATTTACATT 
TTCAAAAATA AAAATCTTCT AAATATTTCC AGCAGCCTTG GTAATAATAG TTCTCAGACC 
ATAAAGGCAA CTAATTACAT GAACCTGTTG TTTTGTGAGG GGAATTCAGT TCCGTTCAGC 
TGGAGAATTA AAGATTATTT GGAAGAAATT ACAGCACAAG CTCAGTACAT TGATAGTGTA 
GAAGGTCCAG AGATGAAGTT TGTAAATATG TTTAGCAATG TGCCTTTGGG ACAATATATA 
AATAACATGG AGGAAAATGA TCAACAAAAG CTGTTCCAGT ACTACAAACG GGACTTTTTA 
CTTCTTACTG TGAATATATC CTCCAGGGAT GAACTCAAGT TTTTAGAGAT TTCGCTTTCC 
GCTTGTATTG AAGAATTTAG AAGAAGTAAA GAAGGAGAAG AGCTGACACT TCCCTGGGTG 
CATCTCGCTT ATCACCATTT TCAGCACAGG TTCCAAATAT TCTCTAGGAT TGTAGCGATC 
AGTCCAACTG TTCTGGAGAG GATGAATGAA GGTTTCCAAG AGATAGAAGA GATTCTAACA 
AATCAGATGG CTATTGATAT ATGGGCTGGA TTTGAATGCT TAGACATTTT GGAAGCCACC 
ATTCTGGACC CAAACCCACA AGCATGGTTG AATGAAGTAA AAAACCTTCA GCTGACAATT 
GAACTCATTT GCTCAGAAAA ATACTTAGAA GGGGAAAGTG ACTGGTGCAC ACAAACAGTT 
GAGCAGATCC GAACCATGTG GCATTGTATC TACGCTATTG CATTATTTAC AGAACATGTC 
ATTTTGGACG AGACTCTGCA GAACCCTGAG ACCCAACAAA AACTAAAAGA GCAAACTATT 
ATGTTTGGCC AGCATATCAA AGGCTTTCCT GAAATAAAGT CAATTGATCC TTATAAGGCA 
GTGATTGAAG TTATTAAGAT GAGCAGAGAG GCAGTAGCTG AATCACTTTC CAGGGAAGAC 
AATGTTCAGT TCCAGCAAAA CTGCAATGGA TTCTTCATTG ACATTCTATG TACTCGGTTC 
TTTGAAGGCA ACTCTCCCCC AGACAAAGAA GTTGTTGATC AGCTGCTTTC TTCTCTTTTC 
ATAACTGGTT CAGAGAATGA TGGAAACGAT CTGTCACCTT TTACTGCTGG AACTAATGAA 
AACCCTGCTA TCCGATCTGT GATCCTGAAG ATGCTCCTCA AGTACAGCTT TGAAGAAACA 
CAGAAATATA TCCAGGATCA CTTAATCAGA ATACAAGGAA GCAACTTGAC CACAGAAATA 
CACATGCTCT TTATCAACTG CCTTGAGGAC TGTATGTATG AAAGGTTTCA AGCTCTGCGT 
GAGGAAGACA AATTGCATTA CCTCAAAGAC CAAGGTGAAT TTCTGAGAAA CTTCCTCCAA 
AATGGTCATC CCCCTAATTC CCAAGAGACC ATGGTTGGGT TCCTGCAAGA TCTTGCCAAG 
GTTAGGTTTG CACTCAATGC AGTAGCGGAA CTGCTCTCTG AACCAGACAA TGTTGTAAAT 
GAGAAGAGGG AATTTCTCCA AAGTGGACAA CATTTTTGTG AACATTCAGC TAATGACTGG 
TACCGAGTGT ATTTGATCCG CAAGATGGGA AATGAATCCG GAAGTGAATA TGTCCAAAAT 
CTTAGTAAAG ATGAAGAGTT TAGATGGTTA TTCCCACATC AAATCTTTGG GGAAAGGGAG 
AACAACGCTA ACCAGCTTGA CCGATTTTTG GTGCATAAGG ATGGTTATAG GGAGTTAAGA 
GAAGGTGTTG GAGAAGTCAT AGTTTCAAGA AAGGAGGAGA GTCTTCAACA AAGACTAGAG 
TTGGATGAAA GTTCTGCCAA GGAACAAGCT GTTTACCTGC TCCTCGCCAT TTTCTCTGAA 
GTAACAATGA GATACAGAGA AGCAAACAGG GTCGTCACTC AAGAAGAGAC TGATAATTTA 
AGGAATTTCA TTCAAAATAC CAATGTTCTC CAGGAGCAGT CAATTCAGCA ATTCTCCCAG 
TCACTCCTAA CAAACTGCCG ACCCCTAGTG AGAGTTGCTC CAGACATGAC AAGTTCTCAT 
TACACTATAG TGTCCCTGGC CGTACATCTT GGTGCTGTGC TTCTGAGCAC TGATAATGAT 
CTGTTTCAAC CACTGAGGAA TTTAGCCTTT TCCCCTGACA GAATGCAGCA ATCATTCCTA 
CCAACAATGC CACAGGACAT GCTAAACAAA GCTAGAGAAG TATTGGGAAA TGAAACTTGG 
TATGTCTGTG ACAACAATCA TTATGTTCCA GTTCCTGGCT GTGGGATGCC AATGAGCTAC 
GCCAAGTGCC TAGAATGTGG AATTGAACTG GGGGGAGTTG ACCACAAAAC TTATAAAGGA 
ATGAGAAAGC TTGAACAGGA CATTGATGAG ACAGGACATA TTTTGGGAGC ACCTGAGACT 
CCAGTGTCAG TTGCGGCAGA AGACAGAGGC ATGAGTGCTC CTGTATTTAA TGTATTGAGG 
CTGGTGACCC ACCTGGCGAT GTTACTAGGG GCAGAACAAA ATCGAGAGAA TGTGAAGACC 
ATGGTGAAAC CAGGAGTGGA AGATATTGGG ACTTTCCTTT TCCGACATCT AGAGAAAAAT 
TTGGAACAAG TAAAGAACTC CCTAGGGGAA AGTGTGGATA ATACGGCCAC TGTACTCCAT 
CTTGTTTTCT GCAGGATGCT CACTATTTCC AATGATCAAC CTGACCAGCC TCTTTTACCT 
TTCAATCATA ATCTGTCAAC AAAGGACGTG AGAAGCAACT GGGAGAGAAA TATGGCGGCA 
TCAGTCATCG CTCCGGTGCT TACGAATTTA AACCAAGATC TTGATGATGT CAATAACTAC 
ATAAGGAATG ACAAGAGAAT AAGTTCTGAC CCAATAATCA GAATCATATA TGGGGAACCG 
TTGCATAACA CTGATATTGT GGCATTGCCA GAAGATGGAC TCATTCCCTA CTCAAAAATA 
TGGAGCTGCA GGACAAGAAT TACTGTTGGG CATCTTCAGC ATATGGTTGA GCAGAAGGAT 
GGGGTGAATA ATCTGCCGAT GCTGTCAACA TTCCTGAAGG ATGAATGTGA ACTCCGCCTG 
GTTAAGTTCC TACCAGAAAT ATTAAATCTT CAGAAAGAGC TGGTAAAAAG ATTCCAGAAC 
TTCCGAGATG TGAACAAACA AACAATTGGG GAATTTTTAA CCAGCATACC TGTTGATGGT 
GCTCGACGTG TATTTGAGAA GAGAATACAA ACTTTCCTAT TTACATGGAA TTGTCTAAGA 
CATTCTCTTC TTATGAAAGG GGAAATAAAA CTCCCAGAAG AAGCCTGTGC CAGTGACCTT 
ACCCTAGCGA GTGACTTTGC CATTCTCCTT CCGAGTCGAC AAGGACAGGG TCTCTGTGCC 
ACAGCATTGG TCAGCTATCT CATCGCTCTG CACAACAGCC TGGTTTATGC TGTGAGACGC 
TTCACCAGAG ATGAGCAGAC ATACTCTATT GGGGCAGCTG AAGCCATGGA TCTACATGTG 
ATCAGTTATG AGGTGGAAAC AGATCTGATG CCAATAATTC TGTCCAACTG TCAATACAAC 
ACCCAAAGAG ATGGGAAAGC CTTAGAGGAG ATTGATCTGC AAAGGATCCA GCGTCAGATC 
ATCAGCCGAT TTCTCCAAGG CAAACCTCGC ATTACCCTTG TAGGGCTGCC TACCATTGTC 
CACTCCCAAG ATCGGAACTA CGAGAACATA TTTAGAGACG TGAGGGAAAA AGTCTTACAG 
GATCCACTGC CAAATTCAGC AATAGAAAAC ATAAGCAAAG ATCTGGATTT GTACAGCGAT 
GTCTGTGATG CGCTTCAAAT CATTGAGACT ATGTTGGGGT TCTTAGCAAC ATCCGGGGGC 
AATCCAGATC TTCCGCTGAC CACTTATGTA GAAGATGTCC TACAGATGAA GGATCAGAGC 
GATGAGAACA TCCTGGAGGC ATTGAAGCAG TGTCACTTGA AGCACACAAT CTCACTATGG 
CAGCTTCTCA CCACTTTACA GTCTGAACAC CGCATACGCC TTAAAAATGA TCCATTTGCA 
GATCTGGATG AAGGGTATAA GCAGGAATTA GATGAGGAGA GTCAGAAGCT ACTGACTGCG 
TTCTTGGCAC AACATGGACT CAACGTCTTT CTTCTGGAAT TACATGAAAT GATCATCTTA 
AAGCTCAAGA AGCAACATGG GGCCAATGAT TTCCAACCTG CATGGGGGTT AAGAGAAGTT 
CTGGGCCCAT TGCTTGATGG GAAAGAGCAA CCATTCCCGG CGCTGGAGGA GGATTTCCCA 
GAGCAGATCT GTTTGGCTCA CTGTGTGGAG AGCTGGAAAG TGGCGGCCGC CATGAAGTGG 
AAACGACATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018087016.1
CDS	16..13407
Translation	MHVCLFFLKQLINLIMGKLYQHLINKNLSAVDAIIKDRLMAGLLCAKLIETYSLPADKDSLVKVCRLLSLEQMLNKNLPIQLEELKRIFSVYEGIEYSLKLFCMRCIDGKIEDWVQILPVYHTFNTPAKPEDSTQLKPEENWAGLDGLPYQTIVIRSKFYNRDVQKYMRSKESLQQAGRTLHRSWLCLLPLENLFTFLDTIPTDFRDAFTAAWYKFHTQSSSSKDKHIEKILTKLLNMAKGPSQGSLQKDSIYLCNISLKLHHLICTQTGLNKNSQLPLLSFKIALKLFGAAQKQEPMKGRETSINEVQSNLNKAVEMLKTWLQDFFGHRFNFRRYGYPTFGDELEFWNNLLMNDDNMDLKWKELLLSDLARKIKEESPVEQIKIFCREHSLLQRLHISIEECFEECAINAVHNAYKSHNNILNQISINNLQHFRKLVSTIIRNSWPKDVQGNPTENNNEILKHMLHWKDFTHIFKLYDPNQPITSQFIEETQKLVTLSDSVLLDTVCRLMHGDILYEHLKLVLGNKNQFLDICRLRETIKVEDVKKVLEWRNEELKTVNCKKTWVENLLKTCQNISKSVRVNTEEIQQILSVNLGAQKLNDLLPVKTLGATEPPEDKVTLFNSFTNSIMSMAESLHTHRHSHVFRMLWNEAVSSLSKRDENLENEEDDELTLDEMQESLFTPCFDKYTKIYEDIRSEEVTFEVLDTYFKTFKNHYQALHKELQTIRQLDPEDNGSWVSGRIRKIEQYHQLREAFESAQIVDDIRDFCKLLGDFKTLNTLQQFGADFSDYKEKPLSFITEDIVKTKEALCEITQERAEALKEALMRKEFIKWVRNSLEDVNELKVFVDLASISAGENDMDVDRVAQFHDAVLGYAPILYELKEEFGFNELMLCLKKLWKALESDPNLQKKLRDSARHIEWLKTVKESHGSVELSSLSLASVINQRGIYIIQEPNENLKINPDSVLRLEIPDDDLEQNQVRTFNLEELKELLSKLMLMSGKGDQGNAEVEKFSEIFSNVQRLAKAYIDLCLSGDILFKTWEATIYCSDEIDIAIRMKFKVTVTAIGEIGATGNIFELLPKICQTMEDILEKRQTFMDEKRSQHYFLNYYTAEQLVFLSQQIQKPDVSEELLVMLSFIKPECTKSDTQQALLAARRKGTSKLLATTNNSALFNFKKSTNVQEKLQIAWKYSMDCMNNLFPGCLDINTLGRSLTELAKKENSPVTRSLHPSLKPGCPNLVLCASSEILSSALAVYMQNLNQPLPSYDEVLLCSPKTTYEEVALFLRRCFTPGYSGKKIYSLLYAEDLSYDTAYRAEQLFYQLQKTKGLDYHLVIICDRERHHLHIPSTFSQYKVQVVPQRLFVEVQKYLLQHFTIDPNVVSAASVFKGVSVGIVTSKRAGVGKSLYVKRLYERLKLEFPNEKPLQKIIRMFQPQVDENKVLQKLLPFLTKENKNVPIIFHIDITSSVNRGISEFLFKLLILQYLKDDEGRMWKRQSCQLYLIEILESSCIISEKQKKPAPQMAQNIFMDFFPKISCCSPNEVLTKVTENQTGQNEDPGMDRKEFESECFQRPFQYLIRFEGKQSLDKFTYKEGSVEGNEAKCLQLFLLHCGVMDPSWAELRNFAWFLNVQLKDSENSVFCKPEFVEDAMQGFKNFVVDFMILMAKDFATPSLQITDESPGRQTCEEVCTNDGDLAPFQIRKKWEAEPHPYIFFNDDRMTITFIGFHLCVNSAGNVDAINPRDKSIIKKNIMTRQLYRDLMLQKVQFDIDFDTLPREEKINMLSRVLGINKPQDPDETYELTMDNTLKILAITMRFRCGIPVVIMGETGCGKTRLIKFICKLWKGNLNAENTTSDAENMKLVKVHGGTSAESINKNVLETQAIALTNKQNKCDTVLFFDEANTTQAISSIKEVLCDHTVDGLPLIENSGLQVIAACNPYRRHTEEMIRRLESAGLGYRVKADETNERLGSIPLRQLVYRVHPLPPSIMPLVWDFGQLNNETEKKYIQQIVQHLANEIEMHQSDTQILTNVLSASQSYMRNRNDECSFVSLRDVERCVEVFRWFYRNSDLLQANIENKKAWSLVLAIGVCYHASLEKKDAYRKAICQYFPEPYRNQRIILEKITSIQDLFLDRIQIRDNIARNLALKENLFMMVICIELRIPLFLVGKPGSSKSLSKTIVADAMQGQASHTELYKHLKQIHLVSFQCSPHSTPEGIIGTFRHCARFQKDKNLNEYASVVVLDEIGLAEDSPKMPLKTLHPLLEDGCIDDETSPYKKVGFIGISNWALDPAKMNRGLFVSRGDPDQNELIESAKGICSSDLLTIKEIDHHFKSFSDAYLKLCEKQDKEFFGLRDFYSLIKMVFTYAKHLKNEPTQLEIATAILRNFSGKDDLDALSIFITNQEANTYREEIKIMDLVMENIRNESADIEARYLLLLTRNYAALQILQQTLAKENQHPEIIFGSSFPKDQEYTQICRNINRVKICMETGQMVVLLNLQNLYESLYDALNQYYVYLAGQKYVDLGLGTHRVKCRVHPKFRLIVIEEKEIVYRDFPIPLINRLEKHYLDLNTVLERGQRAAVEELNDWVKDFTAGDREQLIGQKQDYKPADVLIGYHSDTCASVLLQITGKLKEECGDSDLYMAEVKEEAKATLLNCATPDAVIRLGRKELVDEYFNKQKHGSLVDFVKSHIHSGSKHQIAFTEITTFSRLLMSIDKRILEAETKDDVKSVEVLSLQQFDTEHSFLKKIRSFLKDSTGNKILIIQSDFEDGSQGAHLVASAKYATVNEINNLKKDNTEKTSTFVYFITKLPRIQGGSSYVGFHGGLWQSVHIDDLKEPSDKLPDVTALQNMTISQLFYGATAESQLRYVDGMEGGTDASYDTEPDQIPEVVNTTFLIRSCIQSAVGMITDEENTPSRSTRRIEILVNLLSTEDNLRASLLQTLKVRVYEILKNQEENSYNPNEWLVRAASNLDALQEAGTFRYTLWKRIQAAVIPIFAHLIYLMDCDANLEILVNPETEDFLKSLWIYIFKNKNLLNISSSLGNNSSQTIKATNYMNLLFCEGNSVPFSWRIKDYLEEITAQAQYIDSVEGPEMKFVNMFSNVPLGQYINNMEENDQQKLFQYYKRDFLLLTVNISSRDELKFLEISLSACIEEFRRSKEGEELTLPWVHLAYHHFQHRFQIFSRIVAISPTVLERMNEGFQEIEEILTNQMAIDIWAGFECLDILEATILDPNPQAWLNEVKNLQLTIELICSEKYLEGESDWCTQTVEQIRTMWHCIYAIALFTEHVILDETLQNPETQQKLKEQTIMFGQHIKGFPEIKSIDPYKAVIEVIKMSREAVAESLSREDNVQFQQNCNGFFIDILCTRFFEGNSPPDKEVVDQLLSSLFITGSENDGNDLSPFTAGTNENPAIRSVILKMLLKYSFEETQKYIQDHLIRIQGSNLTTEIHMLFINCLEDCMYERFQALREEDKLHYLKDQGEFLRNFLQNGHPPNSQETMVGFLQDLAKVRFALNAVAELLSEPDNVVNEKREFLQSGQHFCEHSANDWYRVYLIRKMGNESGSEYVQNLSKDEEFRWLFPHQIFGERENNANQLDRFLVHKDGYRELREGVGEVIVSRKEESLQQRLELDESSAKEQAVYLLLAIFSEVTMRYREANRVVTQEETDNLRNFIQNTNVLQEQSIQQFSQSLLTNCRPLVRVAPDMTSSHYTIVSLAVHLGAVLLSTDNDLFQPLRNLAFSPDRMQQSFLPTMPQDMLNKAREVLGNETWYVCDNNHYVPVPGCGMPMSYAKCLECGIELGGVDHKTYKGMRKLEQDIDETGHILGAPETPVSVAAEDRGMSAPVFNVLRLVTHLAMLLGAEQNRENVKTMVKPGVEDIGTFLFRHLEKNLEQVKNSLGESVDNTATVLHLVFCRMLTISNDQPDQPLLPFNHNLSTKDVRSNWERNMAASVIAPVLTNLNQDLDDVNNYIRNDKRISSDPIIRIIYGEPLHNTDIVALPEDGLIPYSKIWSCRTRITVGHLQHMVEQKDGVNNLPMLSTFLKDECELRLVKFLPEILNLQKELVKRFQNFRDVNKQTIGEFLTSIPVDGARRVFEKRIQTFLFTWNCLRHSLLMKGEIKLPEEACASDLTLASDFAILLPSRQGQGLCATALVSYLIALHNSLVYAVRRFTRDEQTYSIGAAEAMDLHVISYEVETDLMPIILSNCQYNTQRDGKALEEIDLQRIQRQIISRFLQGKPRITLVGLPTIVHSQDRNYENIFRDVREKVLQDPLPNSAIENISKDLDLYSDVCDALQIIETMLGFLATSGGNPDLPLTTYVEDVLQMKDQSDENILEALKQCHLKHTISLWQLLTTLQSEHRIRLKNDPFADLDEGYKQELDEESQKLLTAFLAQHGLNVFLLELHEMIILKLKKQHGANDFQPAWGLREVLGPLLDGKEQPFPALEEDFPEQICLAHCVESWKVAAAMKWKRH

Target ORF information:

RefSeq Version	XM_018231527.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin-protein ligase RNF213-like (LOC108699444), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018231527.1

ORF Insert Sequence:

ATGCATGTTT GTTTGTTTTT CTTAAAACAG TTGATTAATT TAATAATGGG AAAACTCTAC 
CAACATCTTA TCAACAAGAA TTTATCAGCA GTGGATGCCA TTATAAAAGA CAGGCTGATG 
GCTGGATTAC TCTGCGCCAA ACTGATAGAA ACCTACTCAC TTCCTGCTGA CAAGGATTCT 
CTTGTAAAGG TTTGCAGACT CCTCTCTTTG GAGCAGATGC TTAACAAAAA CCTACCCATC 
CAACTTGAGG AACTGAAAAG AATCTTCTCA GTGTACGAGG GAATAGAGTA TTCCCTGAAA 
CTATTCTGCA TGAGATGCAT TGATGGTAAA ATTGAAGACT GGGTGCAGAT ATTGCCTGTT 
TATCACACAT TTAACACTCC GGCTAAACCA GAGGATTCCA CACAACTAAA ACCAGAGGAA 
AACTGGGCAG GTTTGGACGG GCTGCCATAT CAAACCATTG TGATACGGTC TAAGTTTTAC 
AATAGAGATG TCCAAAAATA CATGAGGAGT AAGGAGAGTT TACAACAGGC AGGCAGAACT 
TTACACAGAT CTTGGCTGTG TCTATTACCA CTGGAAAACT TGTTCACGTT TTTGGATACA 
ATTCCCACCG ATTTCCGAGA TGCTTTTACC GCTGCCTGGT ACAAATTTCA TACACAATCC 
TCAAGCAGCA AAGATAAGCA CATTGAGAAG ATTCTAACAA AACTTCTGAA TATGGCAAAA 
GGACCGAGCC AGGGGTCACT GCAGAAGGAC TCCATATACC TGTGTAATAT CAGTTTGAAA 
CTTCATCATT TAATCTGCAC TCAAACAGGG CTGAATAAAA ACAGTCAGCT CCCACTTTTG 
TCTTTCAAGA TCGCACTAAA GCTTTTTGGA GCAGCACAGA AACAGGAGCC AATGAAAGGG 
AGGGAGACCA GTATCAATGA AGTGCAAAGC AATTTAAACA AAGCTGTTGA GATGCTGAAG 
ACATGGCTAC AGGACTTCTT TGGACATAGA TTTAATTTTA GACGCTATGG ATATCCAACA 
TTTGGGGATG AACTTGAGTT TTGGAATAAC CTTCTTATGA ATGACGACAA TATGGACCTG 
AAGTGGAAAG AACTGCTTCT CTCAGATCTG GCCAGGAAAA TAAAAGAGGA AAGCCCTGTG 
GAACAGATTA AAATCTTTTG CAGAGAACAT TCACTGTTGC AACGGCTTCA TATCTCCATA 
GAAGAATGCT TTGAGGAATG CGCCATTAAT GCTGTTCATA ATGCATATAA GTCTCATAAC 
AATATTCTCA ACCAAATCTC AATAAACAAT CTACAACATT TTAGAAAACT GGTATCAACA 
ATAATAAGAA ACTCTTGGCC AAAGGATGTG CAGGGAAATC CAACTGAGAA TAATAATGAG 
ATTTTAAAGC ACATGCTGCA CTGGAAGGAT TTCACTCATA TATTCAAACT ATATGATCCT 
AACCAACCAA TCACCTCTCA GTTTATAGAA GAAACTCAAA AGCTTGTTAC TTTGTCAGAT 
TCGGTCCTTC TTGACACCGT TTGTCGCCTA ATGCATGGAG ATATTCTGTA TGAACACCTC 
AAGTTGGTAC TGGGCAATAA AAACCAGTTC TTGGATATAT GTAGACTGAG AGAAACCATA 
AAGGTGGAAG ATGTTAAGAA AGTTCTGGAG TGGAGGAATG AAGAGCTTAA AACAGTGAAC 
TGTAAGAAAA CATGGGTGGA GAACTTGCTG AAAACGTGCC AGAACATCAG CAAGTCTGTC 
CGAGTGAATA CTGAGGAAAT TCAACAAATC CTTTCTGTAA ATCTGGGAGC ACAAAAGTTA 
AATGATTTAT TACCTGTTAA GACACTTGGT GCTACTGAAC CTCCTGAAGA CAAGGTGACA 
TTGTTTAACA GTTTCACTAA TTCAATAATG TCAATGGCCG AGAGTCTTCA CACCCACCGG 
CACAGTCATG TTTTCCGCAT GTTGTGGAAT GAGGCTGTTA GCTCTCTTTC AAAGAGAGAT 
GAAAATTTAG AAAATGAAGA AGATGATGAA CTTACTTTGG ATGAGATGCA GGAATCCTTG 
TTCACACCAT GTTTTGATAA ATACACCAAG ATATACGAGG ACATCAGATC TGAAGAAGTG 
ACCTTTGAAG TCCTGGACAC ATATTTCAAG ACTTTCAAAA ACCATTACCA AGCCCTACAT 
AAAGAACTAC AAACCATACG CCAGTTAGAT CCAGAGGATA ATGGTTCCTG GGTCAGTGGC 
CGAATAAGGA AAATTGAGCA GTATCATCAG TTACGTGAAG CCTTTGAATC AGCCCAGATT 
GTTGATGACA TCAGAGATTT CTGTAAACTT TTGGGAGATT TTAAGACCCT GAATACTCTG 
CAACAATTTG GTGCTGACTT TAGTGATTAC AAGGAAAAAC CTTTATCTTT TATCACTGAA 
GACATTGTAA AGACAAAGGA AGCGCTTTGT GAGATAACAC AAGAACGTGC TGAGGCTCTG 
AAGGAGGCTT TAATGAGAAA GGAATTTATA AAGTGGGTTC GAAACTCACT GGAAGATGTC 
AATGAACTGA AAGTTTTTGT GGATCTGGCC TCCATTTCGG CTGGTGAGAA TGACATGGAT 
GTTGACCGTG TTGCTCAGTT CCATGATGCT GTTTTGGGCT ACGCCCCTAT TTTGTATGAA 
CTCAAAGAGG AATTTGGATT TAATGAATTA ATGTTATGCT TGAAAAAACT TTGGAAAGCC 
CTGGAAAGTG ACCCAAATCT GCAGAAAAAA CTGAGAGACT CTGCCCGTCA CATAGAGTGG 
CTGAAAACTG TAAAAGAAAG TCATGGATCT GTGGAACTCT CATCACTGTC TCTGGCATCT 
GTTATAAACC AAAGAGGCAT CTACATTATT CAAGAGCCCA ATGAGAACCT AAAGATAAAC 
CCAGACAGTG TCCTGAGACT AGAAATTCCT GATGATGATC TGGAACAGAA CCAGGTGCGC 
ACCTTTAATT TAGAAGAACT GAAGGAGCTG TTGAGCAAGC TCATGCTTAT GTCTGGGAAA 
GGTGACCAGG GTAATGCTGA AGTTGAAAAG TTTTCAGAGA TTTTCTCCAA TGTTCAGAGA 
CTTGCCAAGG CCTACATTGA TCTCTGCTTG TCTGGAGACA TTTTATTCAA AACTTGGGAG 
GCCACTATCT ACTGCTCAGA TGAGATTGAT ATTGCTATTA GGATGAAATT TAAAGTAACA 
GTAACAGCCA TTGGGGAGAT AGGAGCAACA GGAAATATTT TTGAACTGCT TCCCAAAATC 
TGCCAAACCA TGGAAGACAT ATTAGAAAAA AGGCAAACTT TTATGGATGA AAAACGTTCT 
CAGCATTACT TTCTAAATTA CTACACCGCA GAGCAGTTGG TCTTCCTTAG CCAGCAGATT 
CAGAAGCCTG ATGTTAGTGA AGAGCTGCTT GTTATGTTAT CCTTTATCAA GCCTGAATGT 
ACAAAATCTG ACACCCAGCA AGCTTTATTA GCTGCACGGA GGAAAGGAAC ATCAAAGCTC 
CTTGCGACTA CTAATAATTC TGCTTTGTTT AACTTTAAAA AATCTACCAA TGTTCAAGAG 
AAACTACAGA TTGCTTGGAA ATACTCCATG GACTGCATGA ACAATTTGTT TCCTGGATGT 
CTGGATATAA ATACACTTGG AAGGTCCTTA ACTGAACTGG CAAAAAAGGA AAATAGCCCA 
GTGACCAGAA GTCTACATCC CAGTCTAAAA CCGGGATGTC CCAATTTGGT ACTCTGTGCA 
TCATCAGAGA TTCTATCTTC AGCTCTTGCT GTATATATGC AGAATTTAAA TCAGCCACTT 
CCATCTTATG ATGAAGTTCT TCTCTGTTCA CCTAAAACTA CTTACGAGGA GGTGGCATTG 
TTTTTGAGAA GATGTTTCAC ACCAGGCTAC AGTGGGAAGA AGATTTACAG TTTGCTGTAT 
GCAGAAGATC TCAGCTATGA TACAGCCTAT AGAGCTGAAC AACTGTTTTA CCAACTGCAA 
AAGACAAAAG GCCTAGACTA CCACCTTGTG ATTATCTGTG ACCGTGAGAG ACATCATTTG 
CATATACCAT CTACCTTTAG CCAATACAAA GTGCAAGTTG TTCCTCAGAG ACTTTTTGTT 
GAAGTTCAAA AATATTTATT GCAACATTTT ACTATTGATC CAAATGTAGT CTCAGCTGCC 
TCCGTTTTTA AAGGCGTGAG TGTGGGAATA GTGACCTCCA AACGAGCAGG AGTTGGAAAG 
TCGCTCTATG TGAAGAGACT ATATGAAAGG CTGAAACTGG AATTTCCAAA TGAAAAGCCT 
CTCCAAAAAA TAATCCGAAT GTTTCAGCCC CAGGTGGACG AAAACAAAGT TCTGCAAAAG 
CTTCTTCCAT TTCTAACCAA GGAAAATAAA AATGTGCCCA TAATATTTCA TATTGATATC 
ACCTCTTCTG TTAACAGGGG AATTTCAGAA TTTCTTTTCA AACTTTTGAT TCTTCAATAT 
CTAAAAGATG ATGAAGGGAG AATGTGGAAG CGCCAGTCTT GTCAGCTTTA CCTTATTGAA 
ATCTTAGAAT CTTCTTGCAT AATTTCTGAG AAGCAGAAAA AACCTGCACC TCAGATGGCG 
CAAAATATTT TTATGGACTT TTTCCCTAAA ATATCATGCT GTTCTCCAAA TGAGGTGCTG 
ACAAAAGTCA CAGAAAATCA GACTGGACAA AATGAAGATC CTGGAATGGA TAGAAAGGAA 
TTTGAGAGTG AATGCTTTCA GCGGCCTTTT CAGTACTTGA TACGTTTTGA AGGGAAACAA 
AGTCTGGATA AATTTACATA CAAGGAAGGT TCCGTAGAAG GAAATGAGGC AAAATGTCTG 
CAGTTGTTCC TTTTGCACTG TGGGGTTATG GACCCATCAT GGGCAGAACT TCGAAATTTT 
GCATGGTTCT TAAATGTTCA GTTAAAAGAC AGTGAGAATT CTGTTTTCTG CAAACCAGAA 
TTTGTGGAAG ATGCTATGCA GGGCTTCAAG AATTTTGTTG TAGACTTTAT GATATTGATG 
GCAAAAGATT TTGCAACTCC TTCATTACAG ATCACTGATG AGAGTCCAGG CAGACAAACT 
TGTGAAGAAG TCTGTACTAA TGATGGAGAC CTTGCTCCAT TTCAGATACG TAAAAAATGG 
GAGGCTGAGC CACATCCCTA CATTTTCTTC AATGATGATC GCATGACAAT AACATTTATC 
GGCTTTCACT TGTGCGTGAA CAGTGCTGGA AATGTTGATG CGATCAATCC TAGAGATAAG 
AGCATTATTA AGAAAAATAT TATGACAAGA CAACTTTATC GAGACTTAAT GCTTCAGAAA 
GTTCAGTTCG ATATAGACTT TGACACTCTA CCTAGGGAGG AAAAGATTAA TATGTTATCC 
AGAGTTTTGG GGATTAATAA ACCGCAGGAT CCAGATGAAA CATATGAGTT GACTATGGAT 
AACACTCTGA AGATCCTGGC TATTACAATG AGGTTCCGGT GTGGAATTCC TGTTGTCATT 
ATGGGAGAAA CAGGTTGTGG AAAAACAAGA CTCATAAAGT TTATTTGTAA ATTATGGAAA 
GGAAACCTGA ATGCAGAAAA TACTACATCT GATGCAGAAA ATATGAAACT TGTAAAGGTT 
CATGGCGGTA CAAGTGCTGA GAGTATTAAC AAAAATGTGC TGGAGACACA AGCCATTGCC 
CTCACTAACA AACAGAACAA ATGTGATACA GTGTTGTTTT TTGATGAAGC AAACACAACT 
CAAGCCATAA GCAGTATCAA AGAAGTCCTG TGTGACCACA CTGTGGATGG CTTGCCTTTA 
ATTGAAAACT CAGGTTTGCA AGTCATTGCT GCATGTAACC CCTACAGGAG GCATACAGAA 
GAAATGATCA GACGATTGGA ATCTGCTGGT TTAGGGTACA GAGTAAAAGC AGATGAAACT 
AATGAACGAC TTGGGTCCAT CCCATTAAGA CAATTAGTCT ATCGTGTTCA TCCATTGCCT 
CCAAGTATAA TGCCACTGGT TTGGGATTTT GGTCAACTAA ATAATGAAAC CGAAAAGAAA 
TACATTCAAC AGATAGTACA GCATTTGGCA AATGAGATTG AAATGCATCA GAGCGATACA 
CAGATATTGA CCAATGTGCT TTCAGCTTCC CAGTCATATA TGAGAAATAG AAATGACGAA 
TGCAGCTTTG TAAGCCTTCG AGATGTTGAA CGTTGTGTAG AAGTGTTCAG GTGGTTCTAC 
CGCAATAGTG ACCTCTTGCA GGCAAATATA GAAAATAAAA AGGCGTGGTC TCTTGTTTTG 
GCCATTGGGG TATGTTACCA TGCCTCATTA GAAAAGAAAG ATGCATACAG AAAGGCAATT 
TGCCAATATT TTCCTGAACC CTACAGAAAC CAAAGAATAA TTCTTGAGAA AATAACAAGT 
ATCCAAGACC TCTTTCTAGA TCGTATACAA ATAAGAGACA ACATTGCCAG GAATCTTGCT 
TTGAAGGAAA ATCTATTCAT GATGGTGATT TGCATTGAGC TCAGAATTCC ATTATTTCTT 
GTTGGAAAGC CTGGAAGCTC AAAATCCCTT TCAAAGACCA TTGTTGCCGA TGCAATGCAA 
GGCCAAGCAT CACACACAGA ACTCTACAAA CATCTTAAGC AAATACACCT TGTATCTTTT 
CAGTGTAGTC CTCACTCAAC TCCAGAAGGC ATCATTGGCA CTTTCAGGCA CTGTGCTCGC 
TTCCAGAAAG ACAAGAACTT AAATGAGTAT GCTTCTGTGG TAGTGCTAGA TGAAATAGGG 
CTGGCAGAAG ACTCTCCTAA AATGCCTTTA AAAACATTGC ACCCTTTGCT GGAGGATGGT 
TGTATAGATG ATGAGACATC ACCTTACAAA AAAGTTGGCT TCATAGGAAT TTCTAACTGG 
GCGCTAGATC CTGCAAAAAT GAATAGAGGC TTATTTGTAT CCCGTGGAGA CCCTGATCAA 
AATGAGCTTA TTGAAAGTGC AAAGGGAATC TGCTCGTCTG ATCTCTTGAC CATTAAGGAA 
ATTGACCATC ATTTCAAAAG TTTTTCCGAT GCTTATCTGA AACTGTGTGA AAAACAAGAT 
AAAGAATTCT TTGGTTTACG AGATTTTTAC AGTTTGATAA AAATGGTGTT TACCTATGCG 
AAACACTTAA AAAATGAGCC AACACAGCTT GAGATTGCAA CAGCAATACT GAGGAATTTC 
AGTGGGAAGG ATGACTTGGA TGCCTTGAGT ATTTTTATAA CAAATCAAGA AGCAAACACA 
TATAGGGAGG AAATTAAAAT AATGGATTTG GTAATGGAGA ACATAAGAAA TGAGAGTGCT 
GATATAGAGG CTCGATACCT GTTGTTATTA ACTAGAAATT ACGCAGCACT GCAGATTCTA 
CAACAGACAC TGGCTAAAGA AAATCAACAT CCTGAGATTA TATTTGGGTC CAGCTTTCCA 
AAAGACCAAG AATATACTCA GATCTGCAGA AACATCAACC GTGTTAAAAT CTGTATGGAA 
ACAGGACAGA TGGTTGTCCT TCTTAATCTC CAAAATCTTT ATGAGAGCCT CTATGATGCA 
TTAAATCAAT ATTATGTCTA TCTAGCTGGA CAAAAATATG TTGACTTGGG TTTAGGCACA 
CATCGGGTAA AATGTAGAGT CCACCCAAAG TTTAGACTGA TAGTCATTGA AGAGAAAGAA 
ATTGTGTACA GAGATTTTCC AATTCCTCTT ATTAACAGAC TTGAGAAACA TTATCTGGAT 
CTAAACACAG TTCTAGAGAG GGGACAAAGA GCCGCTGTAG AGGAATTGAA TGACTGGGTT 
AAAGATTTCA CTGCAGGCGA CAGAGAACAA CTAATTGGCC AGAAACAGGA CTATAAACCT 
GCAGATGTCC TTATTGGTTA CCATTCTGAC ACCTGTGCAT CAGTGTTGTT ACAAATAACA 
GGAAAACTGA AAGAGGAATG TGGTGACAGT GACCTTTACA TGGCAGAAGT GAAAGAGGAG 
GCAAAGGCTA CTCTGCTGAA CTGTGCAACA CCCGATGCTG TCATTCGACT GGGCAGAAAA 
GAGTTGGTCG ATGAGTACTT CAACAAACAG AAACATGGAT CTCTGGTTGA CTTTGTTAAG 
AGCCACATCC ATTCAGGATC TAAACATCAA ATTGCATTTA CAGAGATCAC TACATTTTCA 
CGCCTTTTGA TGTCTATTGA CAAAAGAATT TTGGAAGCCG AGACAAAGGA TGATGTCAAA 
AGTGTAGAGG TGCTGTCATT GCAACAGTTT GATACGGAAC ATTCTTTCCT GAAAAAAATC 
AGGAGTTTTC TAAAAGATTC CACTGGTAAT AAAATACTGA TCATACAGTC AGACTTTGAA 
GATGGATCTC AAGGAGCCCA TTTGGTTGCA TCTGCCAAGT ACGCCACTGT AAATGAAATA 
AACAACTTAA AGAAAGACAA CACTGAGAAG ACATCAACCT TTGTCTATTT TATAACTAAA 
CTTCCTAGAA TACAAGGGGG GTCATCATAT GTGGGATTCC ATGGAGGACT TTGGCAATCT 
GTTCATATTG ATGACTTAAA GGAACCAAGC GATAAGTTGC CAGATGTAAC TGCCCTTCAA 
AACATGACAA TAAGCCAATT ATTTTATGGA GCCACAGCTG AATCACAGTT AAGATATGTT 
GATGGCATGG AAGGTGGCAC AGACGCTAGT TATGACACTG AGCCAGACCA GATACCAGAG 
GTAGTAAACA CCACCTTTCT CATTCGGAGC TGCATACAGA GTGCAGTGGG CATGATTACA 
GATGAAGAGA ATACTCCCAG CCGCAGCACA AGGAGGATTG AAATTCTAGT TAATCTACTG 
AGCACCGAGG ACAATCTAAG AGCTTCCCTT CTACAAACAC TGAAAGTTCG TGTTTATGAA 
ATTCTGAAGA ACCAGGAAGA GAATTCATAT AACCCAAATG AGTGGCTTGT AAGAGCTGCC 
TCCAACCTTG ATGCATTGCA AGAAGCTGGC ACATTCCGAT ACACTCTTTG GAAACGTATT 
CAGGCTGCTG TGATCCCAAT ATTTGCACAT CTCATTTATC TGATGGATTG TGATGCCAAC 
CTGGAGATTC TGGTGAACCC AGAAACTGAG GACTTTCTGA AATCTTTATG GATTTACATT 
TTCAAAAATA AAAATCTTCT AAATATTTCC AGCAGCCTTG GTAATAATAG TTCTCAGACC 
ATAAAGGCAA CTAATTACAT GAACCTGTTG TTTTGTGAGG GGAATTCAGT TCCGTTCAGC 
TGGAGAATTA AAGATTATTT GGAAGAAATT ACAGCACAAG CTCAGTACAT TGATAGTGTA 
GAAGGTCCAG AGATGAAGTT TGTAAATATG TTTAGCAATG TGCCTTTGGG ACAATATATA 
AATAACATGG AGGAAAATGA TCAACAAAAG CTGTTCCAGT ACTACAAACG GGACTTTTTA 
CTTCTTACTG TGAATATATC CTCCAGGGAT GAACTCAAGT TTTTAGAGAT TTCGCTTTCC 
GCTTGTATTG AAGAATTTAG AAGAAGTAAA GAAGGAGAAG AGCTGACACT TCCCTGGGTG 
CATCTCGCTT ATCACCATTT TCAGCACAGG TTCCAAATAT TCTCTAGGAT TGTAGCGATC 
AGTCCAACTG TTCTGGAGAG GATGAATGAA GGTTTCCAAG AGATAGAAGA GATTCTAACA 
AATCAGATGG CTATTGATAT ATGGGCTGGA TTTGAATGCT TAGACATTTT GGAAGCCACC 
ATTCTGGACC CAAACCCACA AGCATGGTTG AATGAAGTAA AAAACCTTCA GCTGACAATT 
GAACTCATTT GCTCAGAAAA ATACTTAGAA GGGGAAAGTG ACTGGTGCAC ACAAACAGTT 
GAGCAGATCC GAACCATGTG GCATTGTATC TACGCTATTG CATTATTTAC AGAACATGTC 
ATTTTGGACG AGACTCTGCA GAACCCTGAG ACCCAACAAA AACTAAAAGA GCAAACTATT 
ATGTTTGGCC AGCATATCAA AGGCTTTCCT GAAATAAAGT CAATTGATCC TTATAAGGCA 
GTGATTGAAG TTATTAAGAT GAGCAGAGAG GCAGTAGCTG AATCACTTTC CAGGGAAGAC 
AATGTTCAGT TCCAGCAAAA CTGCAATGGA TTCTTCATTG ACATTCTATG TACTCGGTTC 
TTTGAAGGCA ACTCTCCCCC AGACAAAGAA GTTGTTGATC AGCTGCTTTC TTCTCTTTTC 
ATAACTGGTT CAGAGAATGA TGGAAACGAT CTGTCACCTT TTACTGCTGG AACTAATGAA 
AACCCTGCTA TCCGATCTGT GATCCTGAAG ATGCTCCTCA AGTACAGCTT TGAAGAAACA 
CAGAAATATA TCCAGGATCA CTTAATCAGA ATACAAGGAA GCAACTTGAC CACAGAAATA 
CACATGCTCT TTATCAACTG CCTTGAGGAC TGTATGTATG AAAGGTTTCA AGCTCTGCGT 
GAGGAAGACA AATTGCATTA CCTCAAAGAC CAAGGTGAAT TTCTGAGAAA CTTCCTCCAA 
AATGGTCATC CCCCTAATTC CCAAGAGACC ATGGTTGGGT TCCTGCAAGA TCTTGCCAAG 
GTTAGGTTTG CACTCAATGC AGTAGCGGAA CTGCTCTCTG AACCAGACAA TGTTGTAAAT 
GAGAAGAGGG AATTTCTCCA AAGTGGACAA CATTTTTGTG AACATTCAGC TAATGACTGG 
TACCGAGTGT ATTTGATCCG CAAGATGGGA AATGAATCCG GAAGTGAATA TGTCCAAAAT 
CTTAGTAAAG ATGAAGAGTT TAGATGGTTA TTCCCACATC AAATCTTTGG GGAAAGGGAG 
AACAACGCTA ACCAGCTTGA CCGATTTTTG GTGCATAAGG ATGGTTATAG GGAGTTAAGA 
GAAGGTGTTG GAGAAGTCAT AGTTTCAAGA AAGGAGGAGA GTCTTCAACA AAGACTAGAG 
TTGGATGAAA GTTCTGCCAA GGAACAAGCT GTTTACCTGC TCCTCGCCAT TTTCTCTGAA 
GTAACAATGA GATACAGAGA AGCAAACAGG GTCGTCACTC AAGAAGAGAC TGATAATTTA 
AGGAATTTCA TTCAAAATAC CAATGTTCTC CAGGAGCAGT CAATTCAGCA ATTCTCCCAG 
TCACTCCTAA CAAACTGCCG ACCCCTAGTG AGAGTTGCTC CAGACATGAC AAGTTCTCAT 
TACACTATAG TGTCCCTGGC CGTACATCTT GGTGCTGTGC TTCTGAGCAC TGATAATGAT 
CTGTTTCAAC CACTGAGGAA TTTAGCCTTT TCCCCTGACA GAATGCAGCA ATCATTCCTA 
CCAACAATGC CACAGGACAT GCTAAACAAA GCTAGAGAAG TATTGGGAAA TGAAACTTGG 
TATGTCTGTG ACAACAATCA TTATGTTCCA GTTCCTGGCT GTGGGATGCC AATGAGCTAC 
GCCAAGTGCC TAGAATGTGG AATTGAACTG GGGGGAGTTG ACCACAAAAC TTATAAAGGA 
ATGAGAAAGC TTGAACAGGA CATTGATGAG ACAGGACATA TTTTGGGAGC ACCTGAGACT 
CCAGTGTCAG TTGCGGCAGA AGACAGAGGC ATGAGTGCTC CTGTATTTAA TGTATTGAGG 
CTGGTGACCC ACCTGGCGAT GTTACTAGGG GCAGAACAAA ATCGAGAGAA TGTGAAGACC 
ATGGTGAAAC CAGGAGTGGA AGATATTGGG ACTTTCCTTT TCCGACATCT AGAGAAAAAT 
TTGGAACAAG TAAAGAACTC CCTAGGGGAA AGTGTGGATA ATACGGCCAC TGTACTCCAT 
CTTGTTTTCT GCAGGATGCT CACTATTTCC AATGATCAAC CTGACCAGCC TCTTTTACCT 
TTCAATCATA ATCTGTCAAC AAAGGACGTG AGAAGCAACT GGGAGAGAAA TATGGCGGCA 
TCAGTCATCG CTCCGGTGCT TACGAATTTA AACCAAGATC TTGATGATGT CAATAACTAC 
ATAAGGAATG ACAAGAGAAT AAGTTCTGAC CCAATAATCA GAATCATATA TGGGGAACCG 
TTGCATAACA CTGATATTGT GGCATTGCCA GAAGATGGAC TCATTCCCTA CTCAAAAATA 
TGGAGCTGCA GGACAAGAAT TACTGTTGGG CATCTTCAGC ATATGGTTGA GCAGAAGGAT 
GGGGTGAATA ATCTGCCGAT GCTGTCAACA TTCCTGAAGG ATGAATGTGA ACTCCGCCTG 
GTTAAGTTCC TACCAGAAAT ATTAAATCTT CAGAAAGAGC TGGTAAAAAG ATTCCAGAAC 
TTCCGAGATG TGAACAAACA AACAATTGGG GAATTTTTAA CCAGCATACC TGTTGATGGT 
GCTCGACGTG TATTTGAGAA GAGAATACAA ACTTTCCTAT TTACATGGAA TTGTCTAAGA 
CATTCTCTTC TTATGAAAGG GGAAATAAAA CTCCCAGAAG AAGCCTGTGC CAGTGACCTT 
ACCCTAGCGA GTGACTTTGC CATTCTCCTT CCGAGTCGAC AAGGACAGGG TCTCTGTGCC 
ACAGCATTGG TCAGCTATCT CATCGCTCTG CACAACAGCC TGGTTTATGC TGTGAGACGC 
TTCACCAGAG ATGAGCAGAC ATACTCTATT GGGGCAGCTG AAGCCATGGA TCTACATGTG 
ATCAGTTATG AGGTGGAAAC AGATCTGATG CCAATAATTC TGTCCAACTG TCAATACAAC 
ACCCAAAGAG ATGGGAAAGC CTTAGAGGAG ATTGATCTGC AAAGGATCCA GCGTCAGATC 
ATCAGCCGAT TTCTCCAAGG CAAACCTCGC ATTACCCTTG TAGGGCTGCC TACCATTGTC 
CACTCCCAAG ATCGGAACTA CGAGAACATA TTTAGAGACG TGAGGGAAAA AGTCTTACAG 
GATCCACTGC CAAATTCAGC AATAGAAAAC ATAAGCAAAG ATCTGGATTT GTACAGCGAT 
GTCTGTGATG CGCTTCAAAT CATTGAGACT ATGTTGGGGT TCTTAGCAAC ATCCGGGGGC 
AATCCAGATC TTCCGCTGAC CACTTATGTA GAAGATGTCC TACAGATGAA GGATCAGAGC 
GATGAGAACA TCCTGGAGGC ATTGAAGCAG TGTCACTTGA AGCACACAAT CTCACTATGG 
CAGCTTCTCA CCACTTTACA GTCTGAACAC CGCATACGCC TTAAAAATGA TCCATTTGCA 
GATCTGGATG AAGGGTATAA GCAGGAATTA GATGAGGAGA GTCAGAAGCT ACTGACTGCG 
TTCTTGGCAC AACATGGACT CAACGTCTTT CTTCTGGAAT TACATGAAAT GATCATCTTA 
AAGCTCAAGA AGCAACATGG GGCCAATGAT TTCCAACCTG CATGGGGGTT AAGAGAAGTT 
CTGGGCCCAT TGCTTGATGG GAAAGAGCAA CCATTCCCGG CGCTGGAGGA GGATTTCCCA 
GAGCAGATCT GTTTGGCTCA CTGTGTGGAG AGCTGGAAAG TGGCGGCCGC CATGAAGTGG 
AAACGACATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108699444 ( XM_018231528.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018087017.1 Xenopus laevis E3 ubiquitin-protein ligase RNF213-like (LOC108699444), transcript variant X2, mRNA.

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CloneID	OAf43053
Clone ID Related Accession (Same CDS sequence)	XM_018231528.1
Accession Version	XM_018231528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase RNF213-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAC TCTACCAACA TCTTATCAAC AAGAATTTAT CAGCAGTGGA TGCCATTATA 
AAAGACAGGC TGATGGCTGG ATTACTCTGC GCCAAACTGA TAGAAACCTA CTCACTTCCT 
GCTGACAAGG ATTCTCTTGT AAAGGTTTGC AGACTCCTCT CTTTGGAGCA GATGCTTAAC 
AAAAACCTAC CCATCCAACT TGAGGAACTG AAAAGAATCT TCTCAGTGTA CGAGGGAATA 
GAGTATTCCC TGAAACTATT CTGCATGAGA TGCATTGATG GTAAAATTGA AGACTGGGTG 
CAGATATTGC CTGTTTATCA CACATTTAAC ACTCCGGCTA AACCAGAGGA TTCCACACAA 
CTAAAACCAG AGGAAAACTG GGCAGGTTTG GACGGGCTGC CATATCAAAC CATTGTGATA 
CGGTCTAAGT TTTACAATAG AGATGTCCAA AAATACATGA GGAGTAAGGA GAGTTTACAA 
CAGGCAGGCA GAACTTTACA CAGATCTTGG CTGTGTCTAT TACCACTGGA AAACTTGTTC 
ACGTTTTTGG ATACAATTCC CACCGATTTC CGAGATGCTT TTACCGCTGC CTGGTACAAA 
TTTCATACAC AATCCTCAAG CAGCAAAGAT AAGCACATTG AGAAGATTCT AACAAAACTT 
CTGAATATGG CAAAAGGACC GAGCCAGGGG TCACTGCAGA AGGACTCCAT ATACCTGTGT 
AATATCAGTT TGAAACTTCA TCATTTAATC TGCACTCAAA CAGGGCTGAA TAAAAACAGT 
CAGCTCCCAC TTTTGTCTTT CAAGATCGCA CTAAAGCTTT TTGGAGCAGC ACAGAAACAG 
GAGCCAATGA AAGGGAGGGA GACCAGTATC AATGAAGTGC AAAGCAATTT AAACAAAGCT 
GTTGAGATGC TGAAGACATG GCTACAGGAC TTCTTTGGAC ATAGATTTAA TTTTAGACGC 
TATGGATATC CAACATTTGG GGATGAACTT GAGTTTTGGA ATAACCTTCT TATGAATGAC 
GACAATATGG ACCTGAAGTG GAAAGAACTG CTTCTCTCAG ATCTGGCCAG GAAAATAAAA 
GAGGAAAGCC CTGTGGAACA GATTAAAATC TTTTGCAGAG AACATTCACT GTTGCAACGG 
CTTCATATCT CCATAGAAGA ATGCTTTGAG GAATGCGCCA TTAATGCTGT TCATAATGCA 
TATAAGTCTC ATAACAATAT TCTCAACCAA ATCTCAATAA ACAATCTACA ACATTTTAGA 
AAACTGGTAT CAACAATAAT AAGAAACTCT TGGCCAAAGG ATGTGCAGGG AAATCCAACT 
GAGAATAATA ATGAGATTTT AAAGCACATG CTGCACTGGA AGGATTTCAC TCATATATTC 
AAACTATATG ATCCTAACCA ACCAATCACC TCTCAGTTTA TAGAAGAAAC TCAAAAGCTT 
GTTACTTTGT CAGATTCGGT CCTTCTTGAC ACCGTTTGTC GCCTAATGCA TGGAGATATT 
CTGTATGAAC ACCTCAAGTT GGTACTGGGC AATAAAAACC AGTTCTTGGA TATATGTAGA 
CTGAGAGAAA CCATAAAGGT GGAAGATGTT AAGAAAGTTC TGGAGTGGAG GAATGAAGAG 
CTTAAAACAG TGAACTGTAA GAAAACATGG GTGGAGAACT TGCTGAAAAC GTGCCAGAAC 
ATCAGCAAGT CTGTCCGAGT GAATACTGAG GAAATTCAAC AAATCCTTTC TGTAAATCTG 
GGAGCACAAA AGTTAAATGA TTTATTACCT GTTAAGACAC TTGGTGCTAC TGAACCTCCT 
GAAGACAAGG TGACATTGTT TAACAGTTTC ACTAATTCAA TAATGTCAAT GGCCGAGAGT 
CTTCACACCC ACCGGCACAG TCATGTTTTC CGCATGTTGT GGAATGAGGC TGTTAGCTCT 
CTTTCAAAGA GAGATGAAAA TTTAGAAAAT GAAGAAGATG ATGAACTTAC TTTGGATGAG 
ATGCAGGAAT CCTTGTTCAC ACCATGTTTT GATAAATACA CCAAGATATA CGAGGACATC 
AGATCTGAAG AAGTGACCTT TGAAGTCCTG GACACATATT TCAAGACTTT CAAAAACCAT 
TACCAAGCCC TACATAAAGA ACTACAAACC ATACGCCAGT TAGATCCAGA GGATAATGGT 
TCCTGGGTCA GTGGCCGAAT AAGGAAAATT GAGCAGTATC ATCAGTTACG TGAAGCCTTT 
GAATCAGCCC AGATTGTTGA TGACATCAGA GATTTCTGTA AACTTTTGGG AGATTTTAAG 
ACCCTGAATA CTCTGCAACA ATTTGGTGCT GACTTTAGTG ATTACAAGGA AAAACCTTTA 
TCTTTTATCA CTGAAGACAT TGTAAAGACA AAGGAAGCGC TTTGTGAGAT AACACAAGAA 
CGTGCTGAGG CTCTGAAGGA GGCTTTAATG AGAAAGGAAT TTATAAAGTG GGTTCGAAAC 
TCACTGGAAG ATGTCAATGA ACTGAAAGTT TTTGTGGATC TGGCCTCCAT TTCGGCTGGT 
GAGAATGACA TGGATGTTGA CCGTGTTGCT CAGTTCCATG ATGCTGTTTT GGGCTACGCC 
CCTATTTTGT ATGAACTCAA AGAGGAATTT GGATTTAATG AATTAATGTT ATGCTTGAAA 
AAACTTTGGA AAGCCCTGGA AAGTGACCCA AATCTGCAGA AAAAACTGAG AGACTCTGCC 
CGTCACATAG AGTGGCTGAA AACTGTAAAA GAAAGTCATG GATCTGTGGA ACTCTCATCA 
CTGTCTCTGG CATCTGTTAT AAACCAAAGA GGCATCTACA TTATTCAAGA GCCCAATGAG 
AACCTAAAGA TAAACCCAGA CAGTGTCCTG AGACTAGAAA TTCCTGATGA TGATCTGGAA 
CAGAACCAGG TGCGCACCTT TAATTTAGAA GAACTGAAGG AGCTGTTGAG CAAGCTCATG 
CTTATGTCTG GGAAAGGTGA CCAGGGTAAT GCTGAAGTTG AAAAGTTTTC AGAGATTTTC 
TCCAATGTTC AGAGACTTGC CAAGGCCTAC ATTGATCTCT GCTTGTCTGG AGACATTTTA 
TTCAAAACTT GGGAGGCCAC TATCTACTGC TCAGATGAGA TTGATATTGC TATTAGGATG 
AAATTTAAAG TAACAGTAAC AGCCATTGGG GAGATAGGAG CAACAGGAAA TATTTTTGAA 
CTGCTTCCCA AAATCTGCCA AACCATGGAA GACATATTAG AAAAAAGGCA AACTTTTATG 
GATGAAAAAC GTTCTCAGCA TTACTTTCTA AATTACTACA CCGCAGAGCA GTTGGTCTTC 
CTTAGCCAGC AGATTCAGAA GCCTGATGTT AGTGAAGAGC TGCTTGTTAT GTTATCCTTT 
ATCAAGCCTG AATGTACAAA ATCTGACACC CAGCAAGCTT TATTAGCTGC ACGGAGGAAA 
GGAACATCAA AGCTCCTTGC GACTACTAAT AATTCTGCTT TGTTTAACTT TAAAAAATCT 
ACCAATGTTC AAGAGAAACT ACAGATTGCT TGGAAATACT CCATGGACTG CATGAACAAT 
TTGTTTCCTG GATGTCTGGA TATAAATACA CTTGGAAGGT CCTTAACTGA ACTGGCAAAA 
AAGGAAAATA GCCCAGTGAC CAGAAGTCTA CATCCCAGTC TAAAACCGGG ATGTCCCAAT 
TTGGTACTCT GTGCATCATC AGAGATTCTA TCTTCAGCTC TTGCTGTATA TATGCAGAAT 
TTAAATCAGC CACTTCCATC TTATGATGAA GTTCTTCTCT GTTCACCTAA AACTACTTAC 
GAGGAGGTGG CATTGTTTTT GAGAAGATGT TTCACACCAG GCTACAGTGG GAAGAAGATT 
TACAGTTTGC TGTATGCAGA AGATCTCAGC TATGATACAG CCTATAGAGC TGAACAACTG 
TTTTACCAAC TGCAAAAGAC AAAAGGCCTA GACTACCACC TTGTGATTAT CTGTGACCGT 
GAGAGACATC ATTTGCATAT ACCATCTACC TTTAGCCAAT ACAAAGTGCA AGTTGTTCCT 
CAGAGACTTT TTGTTGAAGT TCAAAAATAT TTATTGCAAC ATTTTACTAT TGATCCAAAT 
GTAGTCTCAG CTGCCTCCGT TTTTAAAGGC GTGAGTGTGG GAATAGTGAC CTCCAAACGA 
GCAGGAGTTG GAAAGTCGCT CTATGTGAAG AGACTATATG AAAGGCTGAA ACTGGAATTT 
CCAAATGAAA AGCCTCTCCA AAAAATAATC CGAATGTTTC AGCCCCAGGT GGACGAAAAC 
AAAGTTCTGC AAAAGCTTCT TCCATTTCTA ACCAAGGAAA ATAAAAATGT GCCCATAATA 
TTTCATATTG ATATCACCTC TTCTGTTAAC AGGGGAATTT CAGAATTTCT TTTCAAACTT 
TTGATTCTTC AATATCTAAA AGATGATGAA GGGAGAATGT GGAAGCGCCA GTCTTGTCAG 
CTTTACCTTA TTGAAATCTT AGAATCTTCT TGCATAATTT CTGAGAAGCA GAAAAAACCT 
GCACCTCAGA TGGCGCAAAA TATTTTTATG GACTTTTTCC CTAAAATATC ATGCTGTTCT 
CCAAATGAGG TGCTGACAAA AGTCACAGAA AATCAGACTG GACAAAATGA AGATCCTGGA 
ATGGATAGAA AGGAATTTGA GAGTGAATGC TTTCAGCGGC CTTTTCAGTA CTTGATACGT 
TTTGAAGGGA AACAAAGTCT GGATAAATTT ACATACAAGG AAGGTTCCGT AGAAGGAAAT 
GAGGCAAAAT GTCTGCAGTT GTTCCTTTTG CACTGTGGGG TTATGGACCC ATCATGGGCA 
GAACTTCGAA ATTTTGCATG GTTCTTAAAT GTTCAGTTAA AAGACAGTGA GAATTCTGTT 
TTCTGCAAAC CAGAATTTGT GGAAGATGCT ATGCAGGGCT TCAAGAATTT TGTTGTAGAC 
TTTATGATAT TGATGGCAAA AGATTTTGCA ACTCCTTCAT TACAGATCAC TGATGAGAGT 
CCAGGCAGAC AAACTTGTGA AGAAGTCTGT ACTAATGATG GAGACCTTGC TCCATTTCAG 
ATACGTAAAA AATGGGAGGC TGAGCCACAT CCCTACATTT TCTTCAATGA TGATCGCATG 
ACAATAACAT TTATCGGCTT TCACTTGTGC GTGAACAGTG CTGGAAATGT TGATGCGATC 
AATCCTAGAG ATAAGAGCAT TATTAAGAAA AATATTATGA CAAGACAACT TTATCGAGAC 
TTAATGCTTC AGAAAGTTCA GTTCGATATA GACTTTGACA CTCTACCTAG GGAGGAAAAG 
ATTAATATGT TATCCAGAGT TTTGGGGATT AATAAACCGC AGGATCCAGA TGAAACATAT 
GAGTTGACTA TGGATAACAC TCTGAAGATC CTGGCTATTA CAATGAGGTT CCGGTGTGGA 
ATTCCTGTTG TCATTATGGG AGAAACAGGT TGTGGAAAAA CAAGACTCAT AAAGTTTATT 
TGTAAATTAT GGAAAGGAAA CCTGAATGCA GAAAATACTA CATCTGATGC AGAAAATATG 
AAACTTGTAA AGGTTCATGG CGGTACAAGT GCTGAGAGTA TTAACAAAAA TGTGCTGGAG 
ACACAAGCCA TTGCCCTCAC TAACAAACAG AACAAATGTG ATACAGTGTT GTTTTTTGAT 
GAAGCAAACA CAACTCAAGC CATAAGCAGT ATCAAAGAAG TCCTGTGTGA CCACACTGTG 
GATGGCTTGC CTTTAATTGA AAACTCAGGT TTGCAAGTCA TTGCTGCATG TAACCCCTAC 
AGGAGGCATA CAGAAGAAAT GATCAGACGA TTGGAATCTG CTGGTTTAGG GTACAGAGTA 
AAAGCAGATG AAACTAATGA ACGACTTGGG TCCATCCCAT TAAGACAATT AGTCTATCGT 
GTTCATCCAT TGCCTCCAAG TATAATGCCA CTGGTTTGGG ATTTTGGTCA ACTAAATAAT 
GAAACCGAAA AGAAATACAT TCAACAGATA GTACAGCATT TGGCAAATGA GATTGAAATG 
CATCAGAGCG ATACACAGAT ATTGACCAAT GTGCTTTCAG CTTCCCAGTC ATATATGAGA 
AATAGAAATG ACGAATGCAG CTTTGTAAGC CTTCGAGATG TTGAACGTTG TGTAGAAGTG 
TTCAGGTGGT TCTACCGCAA TAGTGACCTC TTGCAGGCAA ATATAGAAAA TAAAAAGGCG 
TGGTCTCTTG TTTTGGCCAT TGGGGTATGT TACCATGCCT CATTAGAAAA GAAAGATGCA 
TACAGAAAGG CAATTTGCCA ATATTTTCCT GAACCCTACA GAAACCAAAG AATAATTCTT 
GAGAAAATAA CAAGTATCCA AGACCTCTTT CTAGATCGTA TACAAATAAG AGACAACATT 
GCCAGGAATC TTGCTTTGAA GGAAAATCTA TTCATGATGG TGATTTGCAT TGAGCTCAGA 
ATTCCATTAT TTCTTGTTGG AAAGCCTGGA AGCTCAAAAT CCCTTTCAAA GACCATTGTT 
GCCGATGCAA TGCAAGGCCA AGCATCACAC ACAGAACTCT ACAAACATCT TAAGCAAATA 
CACCTTGTAT CTTTTCAGTG TAGTCCTCAC TCAACTCCAG AAGGCATCAT TGGCACTTTC 
AGGCACTGTG CTCGCTTCCA GAAAGACAAG AACTTAAATG AGTATGCTTC TGTGGTAGTG 
CTAGATGAAA TAGGGCTGGC AGAAGACTCT CCTAAAATGC CTTTAAAAAC ATTGCACCCT 
TTGCTGGAGG ATGGTTGTAT AGATGATGAG ACATCACCTT ACAAAAAAGT TGGCTTCATA 
GGAATTTCTA ACTGGGCGCT AGATCCTGCA AAAATGAATA GAGGCTTATT TGTATCCCGT 
GGAGACCCTG ATCAAAATGA GCTTATTGAA AGTGCAAAGG GAATCTGCTC GTCTGATCTC 
TTGACCATTA AGGAAATTGA CCATCATTTC AAAAGTTTTT CCGATGCTTA TCTGAAACTG 
TGTGAAAAAC AAGATAAAGA ATTCTTTGGT TTACGAGATT TTTACAGTTT GATAAAAATG 
GTGTTTACCT ATGCGAAACA CTTAAAAAAT GAGCCAACAC AGCTTGAGAT TGCAACAGCA 
ATACTGAGGA ATTTCAGTGG GAAGGATGAC TTGGATGCCT TGAGTATTTT TATAACAAAT 
CAAGAAGCAA ACACATATAG GGAGGAAATT AAAATAATGG ATTTGGTAAT GGAGAACATA 
AGAAATGAGA GTGCTGATAT AGAGGCTCGA TACCTGTTGT TATTAACTAG AAATTACGCA 
GCACTGCAGA TTCTACAACA GACACTGGCT AAAGAAAATC AACATCCTGA GATTATATTT 
GGGTCCAGCT TTCCAAAAGA CCAAGAATAT ACTCAGATCT GCAGAAACAT CAACCGTGTT 
AAAATCTGTA TGGAAACAGG ACAGATGGTT GTCCTTCTTA ATCTCCAAAA TCTTTATGAG 
AGCCTCTATG ATGCATTAAA TCAATATTAT GTCTATCTAG CTGGACAAAA ATATGTTGAC 
TTGGGTTTAG GCACACATCG GGTAAAATGT AGAGTCCACC CAAAGTTTAG ACTGATAGTC 
ATTGAAGAGA AAGAAATTGT GTACAGAGAT TTTCCAATTC CTCTTATTAA CAGACTTGAG 
AAACATTATC TGGATCTAAA CACAGTTCTA GAGAGGGGAC AAAGAGCCGC TGTAGAGGAA 
TTGAATGACT GGGTTAAAGA TTTCACTGCA GGCGACAGAG AACAACTAAT TGGCCAGAAA 
CAGGACTATA AACCTGCAGA TGTCCTTATT GGTTACCATT CTGACACCTG TGCATCAGTG 
TTGTTACAAA TAACAGGAAA ACTGAAAGAG GAATGTGGTG ACAGTGACCT TTACATGGCA 
GAAGTGAAAG AGGAGGCAAA GGCTACTCTG CTGAACTGTG CAACACCCGA TGCTGTCATT 
CGACTGGGCA GAAAAGAGTT GGTCGATGAG TACTTCAACA AACAGAAACA TGGATCTCTG 
GTTGACTTTG TTAAGAGCCA CATCCATTCA GGATCTAAAC ATCAAATTGC ATTTACAGAG 
ATCACTACAT TTTCACGCCT TTTGATGTCT ATTGACAAAA GAATTTTGGA AGCCGAGACA 
AAGGATGATG TCAAAAGTGT AGAGGTGCTG TCATTGCAAC AGTTTGATAC GGAACATTCT 
TTCCTGAAAA AAATCAGGAG TTTTCTAAAA GATTCCACTG GTAATAAAAT ACTGATCATA 
CAGTCAGACT TTGAAGATGG ATCTCAAGGA GCCCATTTGG TTGCATCTGC CAAGTACGCC 
ACTGTAAATG AAATAAACAA CTTAAAGAAA GACAACACTG AGAAGACATC AACCTTTGTC 
TATTTTATAA CTAAACTTCC TAGAATACAA GGGGGGTCAT CATATGTGGG ATTCCATGGA 
GGACTTTGGC AATCTGTTCA TATTGATGAC TTAAAGGAAC CAAGCGATAA GTTGCCAGAT 
GTAACTGCCC TTCAAAACAT GACAATAAGC CAATTATTTT ATGGAGCCAC AGCTGAATCA 
CAGTTAAGAT ATGTTGATGG CATGGAAGGT GGCACAGACG CTAGTTATGA CACTGAGCCA 
GACCAGATAC CAGAGGTAGT AAACACCACC TTTCTCATTC GGAGCTGCAT ACAGAGTGCA 
GTGGGCATGA TTACAGATGA AGAGAATACT CCCAGCCGCA GCACAAGGAG GATTGAAATT 
CTAGTTAATC TACTGAGCAC CGAGGACAAT CTAAGAGCTT CCCTTCTACA AACACTGAAA 
GTTCGTGTTT ATGAAATTCT GAAGAACCAG GAAGAGAATT CATATAACCC AAATGAGTGG 
CTTGTAAGAG CTGCCTCCAA CCTTGATGCA TTGCAAGAAG CTGGCACATT CCGATACACT 
CTTTGGAAAC GTATTCAGGC TGCTGTGATC CCAATATTTG CACATCTCAT TTATCTGATG 
GATTGTGATG CCAACCTGGA GATTCTGGTG AACCCAGAAA CTGAGGACTT TCTGAAATCT 
TTATGGATTT ACATTTTCAA AAATAAAAAT CTTCTAAATA TTTCCAGCAG CCTTGGTAAT 
AATAGTTCTC AGACCATAAA GGCAACTAAT TACATGAACC TGTTGTTTTG TGAGGGGAAT 
TCAGTTCCGT TCAGCTGGAG AATTAAAGAT TATTTGGAAG AAATTACAGC ACAAGCTCAG 
TACATTGATA GTGTAGAAGG TCCAGAGATG AAGTTTGTAA ATATGTTTAG CAATGTGCCT 
TTGGGACAAT ATATAAATAA CATGGAGGAA AATGATCAAC AAAAGCTGTT CCAGTACTAC 
AAACGGGACT TTTTACTTCT TACTGTGAAT ATATCCTCCA GGGATGAACT CAAGTTTTTA 
GAGATTTCGC TTTCCGCTTG TATTGAAGAA TTTAGAAGAA GTAAAGAAGG AGAAGAGCTG 
ACACTTCCCT GGGTGCATCT CGCTTATCAC CATTTTCAGC ACAGGTTCCA AATATTCTCT 
AGGATTGTAG CGATCAGTCC AACTGTTCTG GAGAGGATGA ATGAAGGTTT CCAAGAGATA 
GAAGAGATTC TAACAAATCA GATGGCTATT GATATATGGG CTGGATTTGA ATGCTTAGAC 
ATTTTGGAAG CCACCATTCT GGACCCAAAC CCACAAGCAT GGTTGAATGA AGTAAAAAAC 
CTTCAGCTGA CAATTGAACT CATTTGCTCA GAAAAATACT TAGAAGGGGA AAGTGACTGG 
TGCACACAAA CAGTTGAGCA GATCCGAACC ATGTGGCATT GTATCTACGC TATTGCATTA 
TTTACAGAAC ATGTCATTTT GGACGAGACT CTGCAGAACC CTGAGACCCA ACAAAAACTA 
AAAGAGCAAA CTATTATGTT TGGCCAGCAT ATCAAAGGCT TTCCTGAAAT AAAGTCAATT 
GATCCTTATA AGGCAGTGAT TGAAGTTATT AAGATGAGCA GAGAGGCAGT AGCTGAATCA 
CTTTCCAGGG AAGACAATGT TCAGTTCCAG CAAAACTGCA ATGGATTCTT CATTGACATT 
CTATGTACTC GGTTCTTTGA AGGCAACTCT CCCCCAGACA AAGAAGTTGT TGATCAGCTG 
CTTTCTTCTC TTTTCATAAC TGGTTCAGAG AATGATGGAA ACGATCTGTC ACCTTTTACT 
GCTGGAACTA ATGAAAACCC TGCTATCCGA TCTGTGATCC TGAAGATGCT CCTCAAGTAC 
AGCTTTGAAG AAACACAGAA ATATATCCAG GATCACTTAA TCAGAATACA AGGAAGCAAC 
TTGACCACAG AAATACACAT GCTCTTTATC AACTGCCTTG AGGACTGTAT GTATGAAAGG 
TTTCAAGCTC TGCGTGAGGA AGACAAATTG CATTACCTCA AAGACCAAGG TGAATTTCTG 
AGAAACTTCC TCCAAAATGG TCATCCCCCT AATTCCCAAG AGACCATGGT TGGGTTCCTG 
CAAGATCTTG CCAAGGTTAG GTTTGCACTC AATGCAGTAG CGGAACTGCT CTCTGAACCA 
GACAATGTTG TAAATGAGAA GAGGGAATTT CTCCAAAGTG GACAACATTT TTGTGAACAT 
TCAGCTAATG ACTGGTACCG AGTGTATTTG ATCCGCAAGA TGGGAAATGA ATCCGGAAGT 
GAATATGTCC AAAATCTTAG TAAAGATGAA GAGTTTAGAT GGTTATTCCC ACATCAAATC 
TTTGGGGAAA GGGAGAACAA CGCTAACCAG CTTGACCGAT TTTTGGTGCA TAAGGATGGT 
TATAGGGAGT TAAGAGAAGG TGTTGGAGAA GTCATAGTTT CAAGAAAGGA GGAGAGTCTT 
CAACAAAGAC TAGAGTTGGA TGAAAGTTCT GCCAAGGAAC AAGCTGTTTA CCTGCTCCTC 
GCCATTTTCT CTGAAGTAAC AATGAGATAC AGAGAAGCAA ACAGGGTCGT CACTCAAGAA 
GAGACTGATA ATTTAAGGAA TTTCATTCAA AATACCAATG TTCTCCAGGA GCAGTCAATT 
CAGCAATTCT CCCAGTCACT CCTAACAAAC TGCCGACCCC TAGTGAGAGT TGCTCCAGAC 
ATGACAAGTT CTCATTACAC TATAGTGTCC CTGGCCGTAC ATCTTGGTGC TGTGCTTCTG 
AGCACTGATA ATGATCTGTT TCAACCACTG AGGAATTTAG CCTTTTCCCC TGACAGAATG 
CAGCAATCAT TCCTACCAAC AATGCCACAG GACATGCTAA ACAAAGCTAG AGAAGTATTG 
GGAAATGAAA CTTGGTATGT CTGTGACAAC AATCATTATG TTCCAGTTCC TGGCTGTGGG 
ATGCCAATGA GCTACGCCAA GTGCCTAGAA TGTGGAATTG AACTGGGGGG AGTTGACCAC 
AAAACTTATA AAGGAATGAG AAAGCTTGAA CAGGACATTG ATGAGACAGG ACATATTTTG 
GGAGCACCTG AGACTCCAGT GTCAGTTGCG GCAGAAGACA GAGGCATGAG TGCTCCTGTA 
TTTAATGTAT TGAGGCTGGT GACCCACCTG GCGATGTTAC TAGGGGCAGA ACAAAATCGA 
GAGAATGTGA AGACCATGGT GAAACCAGGA GTGGAAGATA TTGGGACTTT CCTTTTCCGA 
CATCTAGAGA AAAATTTGGA ACAAGTAAAG AACTCCCTAG GGGAAAGTGT GGATAATACG 
GCCACTGTAC TCCATCTTGT TTTCTGCAGG ATGCTCACTA TTTCCAATGA TCAACCTGAC 
CAGCCTCTTT TACCTTTCAA TCATAATCTG TCAACAAAGG ACGTGAGAAG CAACTGGGAG 
AGAAATATGG CGGCATCAGT CATCGCTCCG GTGCTTACGA ATTTAAACCA AGATCTTGAT 
GATGTCAATA ACTACATAAG GAATGACAAG AGAATAAGTT CTGACCCAAT AATCAGAATC 
ATATATGGGG AACCGTTGCA TAACACTGAT ATTGTGGCAT TGCCAGAAGA TGGACTCATT 
CCCTACTCAA AAATATGGAG CTGCAGGACA AGAATTACTG TTGGGCATCT TCAGCATATG 
GTTGAGCAGA AGGATGGGGT GAATAATCTG CCGATGCTGT CAACATTCCT GAAGGATGAA 
TGTGAACTCC GCCTGGTTAA GTTCCTACCA GAAATATTAA ATCTTCAGAA AGAGCTGGTA 
AAAAGATTCC AGAACTTCCG AGATGTGAAC AAACAAACAA TTGGGGAATT TTTAACCAGC 
ATACCTGTTG ATGGTGCTCG ACGTGTATTT GAGAAGAGAA TACAAACTTT CCTATTTACA 
TGGAATTGTC TAAGACATTC TCTTCTTATG AAAGGGGAAA TAAAACTCCC AGAAGAAGCC 
TGTGCCAGTG ACCTTACCCT AGCGAGTGAC TTTGCCATTC TCCTTCCGAG TCGACAAGGA 
CAGGGTCTCT GTGCCACAGC ATTGGTCAGC TATCTCATCG CTCTGCACAA CAGCCTGGTT 
TATGCTGTGA GACGCTTCAC CAGAGATGAG CAGACGTATT CTATTGGGGC AGCTGAAGCC 
ATGGATCTAC ATGTGATCAG TTATGAGGTG GAAACAGATC TGATGCCAAT AATTCTGTCC 
AACTGTCAAT ACAACACCCA AAGAGATGGG AAAGCCTTAG AGGAGATTGA TCTGCAAAGG 
ATCCAGCGTC AGATCATCAG CCGATTTCTC CAAGGCAAAC CTCGCATTAC CCTTGTAGGG 
CTGCCTACCA TTGTCCACTC CCAAGATCGG AACTACGAGA ACATATTTAG AGACGTGAGG 
GAAAAAGTCT TACAGGATCC ACTGCCAAAT TCAGCAATAG AAAACATAAG CAAAGATCTG 
GATTTGTACA GCGATGTCTG TGATGCGCTT CAAATCATTG AGACTATGTT GGGGTTCTTA 
GCAACATCCG GGGGCAATCC AGATCTTCCG CTGACCACTT ATGTAGAAGA TGTCCTACAG 
ATGAAGGATC AGAGCGATGA GAACATCCTG GAGGCATTGA AGCAGTGTCA CTTGAAGCAC 
ACAATCTCAC TATGGCAGCT TCTCACCACT TTACAGTCTG AACACCGCAT ACGCCTTAAA 
AATGATCCAT TTGCAGATCT GGATGAAGGG TATAAGCAGG AATTAGATGA GGAGAGTCAG 
AAGCTACTGA CTGCGTTCTT GGCACAACAT GGACTCAACG TCTTTCTTCT GGAATTACAT 
GAAATGATCA TCTTAAAGCT CAAGAAGCAA CATGGGGCCA ATGATTTCCA ACCTGCATGG 
GGGTTAAGAG AAGTTCTGGG CCCATTGCTT GATGGGAAAG AGCAACCATT CCCGGCGCTG 
GAGGAGGATT TCCCAGAGCA GATCTGTTTG GCTCACTGTG TGGAGAGCTG GAAAGTGGCG 
GCCGCCATGA AGTGGAAACG ACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018087017.1
CDS	32..13378
Translation	MGKLYQHLINKNLSAVDAIIKDRLMAGLLCAKLIETYSLPADKDSLVKVCRLLSLEQMLNKNLPIQLEELKRIFSVYEGIEYSLKLFCMRCIDGKIEDWVQILPVYHTFNTPAKPEDSTQLKPEENWAGLDGLPYQTIVIRSKFYNRDVQKYMRSKESLQQAGRTLHRSWLCLLPLENLFTFLDTIPTDFRDAFTAAWYKFHTQSSSSKDKHIEKILTKLLNMAKGPSQGSLQKDSIYLCNISLKLHHLICTQTGLNKNSQLPLLSFKIALKLFGAAQKQEPMKGRETSINEVQSNLNKAVEMLKTWLQDFFGHRFNFRRYGYPTFGDELEFWNNLLMNDDNMDLKWKELLLSDLARKIKEESPVEQIKIFCREHSLLQRLHISIEECFEECAINAVHNAYKSHNNILNQISINNLQHFRKLVSTIIRNSWPKDVQGNPTENNNEILKHMLHWKDFTHIFKLYDPNQPITSQFIEETQKLVTLSDSVLLDTVCRLMHGDILYEHLKLVLGNKNQFLDICRLRETIKVEDVKKVLEWRNEELKTVNCKKTWVENLLKTCQNISKSVRVNTEEIQQILSVNLGAQKLNDLLPVKTLGATEPPEDKVTLFNSFTNSIMSMAESLHTHRHSHVFRMLWNEAVSSLSKRDENLENEEDDELTLDEMQESLFTPCFDKYTKIYEDIRSEEVTFEVLDTYFKTFKNHYQALHKELQTIRQLDPEDNGSWVSGRIRKIEQYHQLREAFESAQIVDDIRDFCKLLGDFKTLNTLQQFGADFSDYKEKPLSFITEDIVKTKEALCEITQERAEALKEALMRKEFIKWVRNSLEDVNELKVFVDLASISAGENDMDVDRVAQFHDAVLGYAPILYELKEEFGFNELMLCLKKLWKALESDPNLQKKLRDSARHIEWLKTVKESHGSVELSSLSLASVINQRGIYIIQEPNENLKINPDSVLRLEIPDDDLEQNQVRTFNLEELKELLSKLMLMSGKGDQGNAEVEKFSEIFSNVQRLAKAYIDLCLSGDILFKTWEATIYCSDEIDIAIRMKFKVTVTAIGEIGATGNIFELLPKICQTMEDILEKRQTFMDEKRSQHYFLNYYTAEQLVFLSQQIQKPDVSEELLVMLSFIKPECTKSDTQQALLAARRKGTSKLLATTNNSALFNFKKSTNVQEKLQIAWKYSMDCMNNLFPGCLDINTLGRSLTELAKKENSPVTRSLHPSLKPGCPNLVLCASSEILSSALAVYMQNLNQPLPSYDEVLLCSPKTTYEEVALFLRRCFTPGYSGKKIYSLLYAEDLSYDTAYRAEQLFYQLQKTKGLDYHLVIICDRERHHLHIPSTFSQYKVQVVPQRLFVEVQKYLLQHFTIDPNVVSAASVFKGVSVGIVTSKRAGVGKSLYVKRLYERLKLEFPNEKPLQKIIRMFQPQVDENKVLQKLLPFLTKENKNVPIIFHIDITSSVNRGISEFLFKLLILQYLKDDEGRMWKRQSCQLYLIEILESSCIISEKQKKPAPQMAQNIFMDFFPKISCCSPNEVLTKVTENQTGQNEDPGMDRKEFESECFQRPFQYLIRFEGKQSLDKFTYKEGSVEGNEAKCLQLFLLHCGVMDPSWAELRNFAWFLNVQLKDSENSVFCKPEFVEDAMQGFKNFVVDFMILMAKDFATPSLQITDESPGRQTCEEVCTNDGDLAPFQIRKKWEAEPHPYIFFNDDRMTITFIGFHLCVNSAGNVDAINPRDKSIIKKNIMTRQLYRDLMLQKVQFDIDFDTLPREEKINMLSRVLGINKPQDPDETYELTMDNTLKILAITMRFRCGIPVVIMGETGCGKTRLIKFICKLWKGNLNAENTTSDAENMKLVKVHGGTSAESINKNVLETQAIALTNKQNKCDTVLFFDEANTTQAISSIKEVLCDHTVDGLPLIENSGLQVIAACNPYRRHTEEMIRRLESAGLGYRVKADETNERLGSIPLRQLVYRVHPLPPSIMPLVWDFGQLNNETEKKYIQQIVQHLANEIEMHQSDTQILTNVLSASQSYMRNRNDECSFVSLRDVERCVEVFRWFYRNSDLLQANIENKKAWSLVLAIGVCYHASLEKKDAYRKAICQYFPEPYRNQRIILEKITSIQDLFLDRIQIRDNIARNLALKENLFMMVICIELRIPLFLVGKPGSSKSLSKTIVADAMQGQASHTELYKHLKQIHLVSFQCSPHSTPEGIIGTFRHCARFQKDKNLNEYASVVVLDEIGLAEDSPKMPLKTLHPLLEDGCIDDETSPYKKVGFIGISNWALDPAKMNRGLFVSRGDPDQNELIESAKGICSSDLLTIKEIDHHFKSFSDAYLKLCEKQDKEFFGLRDFYSLIKMVFTYAKHLKNEPTQLEIATAILRNFSGKDDLDALSIFITNQEANTYREEIKIMDLVMENIRNESADIEARYLLLLTRNYAALQILQQTLAKENQHPEIIFGSSFPKDQEYTQICRNINRVKICMETGQMVVLLNLQNLYESLYDALNQYYVYLAGQKYVDLGLGTHRVKCRVHPKFRLIVIEEKEIVYRDFPIPLINRLEKHYLDLNTVLERGQRAAVEELNDWVKDFTAGDREQLIGQKQDYKPADVLIGYHSDTCASVLLQITGKLKEECGDSDLYMAEVKEEAKATLLNCATPDAVIRLGRKELVDEYFNKQKHGSLVDFVKSHIHSGSKHQIAFTEITTFSRLLMSIDKRILEAETKDDVKSVEVLSLQQFDTEHSFLKKIRSFLKDSTGNKILIIQSDFEDGSQGAHLVASAKYATVNEINNLKKDNTEKTSTFVYFITKLPRIQGGSSYVGFHGGLWQSVHIDDLKEPSDKLPDVTALQNMTISQLFYGATAESQLRYVDGMEGGTDASYDTEPDQIPEVVNTTFLIRSCIQSAVGMITDEENTPSRSTRRIEILVNLLSTEDNLRASLLQTLKVRVYEILKNQEENSYNPNEWLVRAASNLDALQEAGTFRYTLWKRIQAAVIPIFAHLIYLMDCDANLEILVNPETEDFLKSLWIYIFKNKNLLNISSSLGNNSSQTIKATNYMNLLFCEGNSVPFSWRIKDYLEEITAQAQYIDSVEGPEMKFVNMFSNVPLGQYINNMEENDQQKLFQYYKRDFLLLTVNISSRDELKFLEISLSACIEEFRRSKEGEELTLPWVHLAYHHFQHRFQIFSRIVAISPTVLERMNEGFQEIEEILTNQMAIDIWAGFECLDILEATILDPNPQAWLNEVKNLQLTIELICSEKYLEGESDWCTQTVEQIRTMWHCIYAIALFTEHVILDETLQNPETQQKLKEQTIMFGQHIKGFPEIKSIDPYKAVIEVIKMSREAVAESLSREDNVQFQQNCNGFFIDILCTRFFEGNSPPDKEVVDQLLSSLFITGSENDGNDLSPFTAGTNENPAIRSVILKMLLKYSFEETQKYIQDHLIRIQGSNLTTEIHMLFINCLEDCMYERFQALREEDKLHYLKDQGEFLRNFLQNGHPPNSQETMVGFLQDLAKVRFALNAVAELLSEPDNVVNEKREFLQSGQHFCEHSANDWYRVYLIRKMGNESGSEYVQNLSKDEEFRWLFPHQIFGERENNANQLDRFLVHKDGYRELREGVGEVIVSRKEESLQQRLELDESSAKEQAVYLLLAIFSEVTMRYREANRVVTQEETDNLRNFIQNTNVLQEQSIQQFSQSLLTNCRPLVRVAPDMTSSHYTIVSLAVHLGAVLLSTDNDLFQPLRNLAFSPDRMQQSFLPTMPQDMLNKAREVLGNETWYVCDNNHYVPVPGCGMPMSYAKCLECGIELGGVDHKTYKGMRKLEQDIDETGHILGAPETPVSVAAEDRGMSAPVFNVLRLVTHLAMLLGAEQNRENVKTMVKPGVEDIGTFLFRHLEKNLEQVKNSLGESVDNTATVLHLVFCRMLTISNDQPDQPLLPFNHNLSTKDVRSNWERNMAASVIAPVLTNLNQDLDDVNNYIRNDKRISSDPIIRIIYGEPLHNTDIVALPEDGLIPYSKIWSCRTRITVGHLQHMVEQKDGVNNLPMLSTFLKDECELRLVKFLPEILNLQKELVKRFQNFRDVNKQTIGEFLTSIPVDGARRVFEKRIQTFLFTWNCLRHSLLMKGEIKLPEEACASDLTLASDFAILLPSRQGQGLCATALVSYLIALHNSLVYAVRRFTRDEQTYSIGAAEAMDLHVISYEVETDLMPIILSNCQYNTQRDGKALEEIDLQRIQRQIISRFLQGKPRITLVGLPTIVHSQDRNYENIFRDVREKVLQDPLPNSAIENISKDLDLYSDVCDALQIIETMLGFLATSGGNPDLPLTTYVEDVLQMKDQSDENILEALKQCHLKHTISLWQLLTTLQSEHRIRLKNDPFADLDEGYKQELDEESQKLLTAFLAQHGLNVFLLELHEMIILKLKKQHGANDFQPAWGLREVLGPLLDGKEQPFPALEEDFPEQICLAHCVESWKVAAAMKWKRH

Target ORF information:

RefSeq Version	XM_018231528.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin-protein ligase RNF213-like (LOC108699444), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018231528.1

ORF Insert Sequence:

ATGGGAAAAC TCTACCAACA TCTTATCAAC AAGAATTTAT CAGCAGTGGA TGCCATTATA 
AAAGACAGGC TGATGGCTGG ATTACTCTGC GCCAAACTGA TAGAAACCTA CTCACTTCCT 
GCTGACAAGG ATTCTCTTGT AAAGGTTTGC AGACTCCTCT CTTTGGAGCA GATGCTTAAC 
AAAAACCTAC CCATCCAACT TGAGGAACTG AAAAGAATCT TCTCAGTGTA CGAGGGAATA 
GAGTATTCCC TGAAACTATT CTGCATGAGA TGCATTGATG GTAAAATTGA AGACTGGGTG 
CAGATATTGC CTGTTTATCA CACATTTAAC ACTCCGGCTA AACCAGAGGA TTCCACACAA 
CTAAAACCAG AGGAAAACTG GGCAGGTTTG GACGGGCTGC CATATCAAAC CATTGTGATA 
CGGTCTAAGT TTTACAATAG AGATGTCCAA AAATACATGA GGAGTAAGGA GAGTTTACAA 
CAGGCAGGCA GAACTTTACA CAGATCTTGG CTGTGTCTAT TACCACTGGA AAACTTGTTC 
ACGTTTTTGG ATACAATTCC CACCGATTTC CGAGATGCTT TTACCGCTGC CTGGTACAAA 
TTTCATACAC AATCCTCAAG CAGCAAAGAT AAGCACATTG AGAAGATTCT AACAAAACTT 
CTGAATATGG CAAAAGGACC GAGCCAGGGG TCACTGCAGA AGGACTCCAT ATACCTGTGT 
AATATCAGTT TGAAACTTCA TCATTTAATC TGCACTCAAA CAGGGCTGAA TAAAAACAGT 
CAGCTCCCAC TTTTGTCTTT CAAGATCGCA CTAAAGCTTT TTGGAGCAGC ACAGAAACAG 
GAGCCAATGA AAGGGAGGGA GACCAGTATC AATGAAGTGC AAAGCAATTT AAACAAAGCT 
GTTGAGATGC TGAAGACATG GCTACAGGAC TTCTTTGGAC ATAGATTTAA TTTTAGACGC 
TATGGATATC CAACATTTGG GGATGAACTT GAGTTTTGGA ATAACCTTCT TATGAATGAC 
GACAATATGG ACCTGAAGTG GAAAGAACTG CTTCTCTCAG ATCTGGCCAG GAAAATAAAA 
GAGGAAAGCC CTGTGGAACA GATTAAAATC TTTTGCAGAG AACATTCACT GTTGCAACGG 
CTTCATATCT CCATAGAAGA ATGCTTTGAG GAATGCGCCA TTAATGCTGT TCATAATGCA 
TATAAGTCTC ATAACAATAT TCTCAACCAA ATCTCAATAA ACAATCTACA ACATTTTAGA 
AAACTGGTAT CAACAATAAT AAGAAACTCT TGGCCAAAGG ATGTGCAGGG AAATCCAACT 
GAGAATAATA ATGAGATTTT AAAGCACATG CTGCACTGGA AGGATTTCAC TCATATATTC 
AAACTATATG ATCCTAACCA ACCAATCACC TCTCAGTTTA TAGAAGAAAC TCAAAAGCTT 
GTTACTTTGT CAGATTCGGT CCTTCTTGAC ACCGTTTGTC GCCTAATGCA TGGAGATATT 
CTGTATGAAC ACCTCAAGTT GGTACTGGGC AATAAAAACC AGTTCTTGGA TATATGTAGA 
CTGAGAGAAA CCATAAAGGT GGAAGATGTT AAGAAAGTTC TGGAGTGGAG GAATGAAGAG 
CTTAAAACAG TGAACTGTAA GAAAACATGG GTGGAGAACT TGCTGAAAAC GTGCCAGAAC 
ATCAGCAAGT CTGTCCGAGT GAATACTGAG GAAATTCAAC AAATCCTTTC TGTAAATCTG 
GGAGCACAAA AGTTAAATGA TTTATTACCT GTTAAGACAC TTGGTGCTAC TGAACCTCCT 
GAAGACAAGG TGACATTGTT TAACAGTTTC ACTAATTCAA TAATGTCAAT GGCCGAGAGT 
CTTCACACCC ACCGGCACAG TCATGTTTTC CGCATGTTGT GGAATGAGGC TGTTAGCTCT 
CTTTCAAAGA GAGATGAAAA TTTAGAAAAT GAAGAAGATG ATGAACTTAC TTTGGATGAG 
ATGCAGGAAT CCTTGTTCAC ACCATGTTTT GATAAATACA CCAAGATATA CGAGGACATC 
AGATCTGAAG AAGTGACCTT TGAAGTCCTG GACACATATT TCAAGACTTT CAAAAACCAT 
TACCAAGCCC TACATAAAGA ACTACAAACC ATACGCCAGT TAGATCCAGA GGATAATGGT 
TCCTGGGTCA GTGGCCGAAT AAGGAAAATT GAGCAGTATC ATCAGTTACG TGAAGCCTTT 
GAATCAGCCC AGATTGTTGA TGACATCAGA GATTTCTGTA AACTTTTGGG AGATTTTAAG 
ACCCTGAATA CTCTGCAACA ATTTGGTGCT GACTTTAGTG ATTACAAGGA AAAACCTTTA 
TCTTTTATCA CTGAAGACAT TGTAAAGACA AAGGAAGCGC TTTGTGAGAT AACACAAGAA 
CGTGCTGAGG CTCTGAAGGA GGCTTTAATG AGAAAGGAAT TTATAAAGTG GGTTCGAAAC 
TCACTGGAAG ATGTCAATGA ACTGAAAGTT TTTGTGGATC TGGCCTCCAT TTCGGCTGGT 
GAGAATGACA TGGATGTTGA CCGTGTTGCT CAGTTCCATG ATGCTGTTTT GGGCTACGCC 
CCTATTTTGT ATGAACTCAA AGAGGAATTT GGATTTAATG AATTAATGTT ATGCTTGAAA 
AAACTTTGGA AAGCCCTGGA AAGTGACCCA AATCTGCAGA AAAAACTGAG AGACTCTGCC 
CGTCACATAG AGTGGCTGAA AACTGTAAAA GAAAGTCATG GATCTGTGGA ACTCTCATCA 
CTGTCTCTGG CATCTGTTAT AAACCAAAGA GGCATCTACA TTATTCAAGA GCCCAATGAG 
AACCTAAAGA TAAACCCAGA CAGTGTCCTG AGACTAGAAA TTCCTGATGA TGATCTGGAA 
CAGAACCAGG TGCGCACCTT TAATTTAGAA GAACTGAAGG AGCTGTTGAG CAAGCTCATG 
CTTATGTCTG GGAAAGGTGA CCAGGGTAAT GCTGAAGTTG AAAAGTTTTC AGAGATTTTC 
TCCAATGTTC AGAGACTTGC CAAGGCCTAC ATTGATCTCT GCTTGTCTGG AGACATTTTA 
TTCAAAACTT GGGAGGCCAC TATCTACTGC TCAGATGAGA TTGATATTGC TATTAGGATG 
AAATTTAAAG TAACAGTAAC AGCCATTGGG GAGATAGGAG CAACAGGAAA TATTTTTGAA 
CTGCTTCCCA AAATCTGCCA AACCATGGAA GACATATTAG AAAAAAGGCA AACTTTTATG 
GATGAAAAAC GTTCTCAGCA TTACTTTCTA AATTACTACA CCGCAGAGCA GTTGGTCTTC 
CTTAGCCAGC AGATTCAGAA GCCTGATGTT AGTGAAGAGC TGCTTGTTAT GTTATCCTTT 
ATCAAGCCTG AATGTACAAA ATCTGACACC CAGCAAGCTT TATTAGCTGC ACGGAGGAAA 
GGAACATCAA AGCTCCTTGC GACTACTAAT AATTCTGCTT TGTTTAACTT TAAAAAATCT 
ACCAATGTTC AAGAGAAACT ACAGATTGCT TGGAAATACT CCATGGACTG CATGAACAAT 
TTGTTTCCTG GATGTCTGGA TATAAATACA CTTGGAAGGT CCTTAACTGA ACTGGCAAAA 
AAGGAAAATA GCCCAGTGAC CAGAAGTCTA CATCCCAGTC TAAAACCGGG ATGTCCCAAT 
TTGGTACTCT GTGCATCATC AGAGATTCTA TCTTCAGCTC TTGCTGTATA TATGCAGAAT 
TTAAATCAGC CACTTCCATC TTATGATGAA GTTCTTCTCT GTTCACCTAA AACTACTTAC 
GAGGAGGTGG CATTGTTTTT GAGAAGATGT TTCACACCAG GCTACAGTGG GAAGAAGATT 
TACAGTTTGC TGTATGCAGA AGATCTCAGC TATGATACAG CCTATAGAGC TGAACAACTG 
TTTTACCAAC TGCAAAAGAC AAAAGGCCTA GACTACCACC TTGTGATTAT CTGTGACCGT 
GAGAGACATC ATTTGCATAT ACCATCTACC TTTAGCCAAT ACAAAGTGCA AGTTGTTCCT 
CAGAGACTTT TTGTTGAAGT TCAAAAATAT TTATTGCAAC ATTTTACTAT TGATCCAAAT 
GTAGTCTCAG CTGCCTCCGT TTTTAAAGGC GTGAGTGTGG GAATAGTGAC CTCCAAACGA 
GCAGGAGTTG GAAAGTCGCT CTATGTGAAG AGACTATATG AAAGGCTGAA ACTGGAATTT 
CCAAATGAAA AGCCTCTCCA AAAAATAATC CGAATGTTTC AGCCCCAGGT GGACGAAAAC 
AAAGTTCTGC AAAAGCTTCT TCCATTTCTA ACCAAGGAAA ATAAAAATGT GCCCATAATA 
TTTCATATTG ATATCACCTC TTCTGTTAAC AGGGGAATTT CAGAATTTCT TTTCAAACTT 
TTGATTCTTC AATATCTAAA AGATGATGAA GGGAGAATGT GGAAGCGCCA GTCTTGTCAG 
CTTTACCTTA TTGAAATCTT AGAATCTTCT TGCATAATTT CTGAGAAGCA GAAAAAACCT 
GCACCTCAGA TGGCGCAAAA TATTTTTATG GACTTTTTCC CTAAAATATC ATGCTGTTCT 
CCAAATGAGG TGCTGACAAA AGTCACAGAA AATCAGACTG GACAAAATGA AGATCCTGGA 
ATGGATAGAA AGGAATTTGA GAGTGAATGC TTTCAGCGGC CTTTTCAGTA CTTGATACGT 
TTTGAAGGGA AACAAAGTCT GGATAAATTT ACATACAAGG AAGGTTCCGT AGAAGGAAAT 
GAGGCAAAAT GTCTGCAGTT GTTCCTTTTG CACTGTGGGG TTATGGACCC ATCATGGGCA 
GAACTTCGAA ATTTTGCATG GTTCTTAAAT GTTCAGTTAA AAGACAGTGA GAATTCTGTT 
TTCTGCAAAC CAGAATTTGT GGAAGATGCT ATGCAGGGCT TCAAGAATTT TGTTGTAGAC 
TTTATGATAT TGATGGCAAA AGATTTTGCA ACTCCTTCAT TACAGATCAC TGATGAGAGT 
CCAGGCAGAC AAACTTGTGA AGAAGTCTGT ACTAATGATG GAGACCTTGC TCCATTTCAG 
ATACGTAAAA AATGGGAGGC TGAGCCACAT CCCTACATTT TCTTCAATGA TGATCGCATG 
ACAATAACAT TTATCGGCTT TCACTTGTGC GTGAACAGTG CTGGAAATGT TGATGCGATC 
AATCCTAGAG ATAAGAGCAT TATTAAGAAA AATATTATGA CAAGACAACT TTATCGAGAC 
TTAATGCTTC AGAAAGTTCA GTTCGATATA GACTTTGACA CTCTACCTAG GGAGGAAAAG 
ATTAATATGT TATCCAGAGT TTTGGGGATT AATAAACCGC AGGATCCAGA TGAAACATAT 
GAGTTGACTA TGGATAACAC TCTGAAGATC CTGGCTATTA CAATGAGGTT CCGGTGTGGA 
ATTCCTGTTG TCATTATGGG AGAAACAGGT TGTGGAAAAA CAAGACTCAT AAAGTTTATT 
TGTAAATTAT GGAAAGGAAA CCTGAATGCA GAAAATACTA CATCTGATGC AGAAAATATG 
AAACTTGTAA AGGTTCATGG CGGTACAAGT GCTGAGAGTA TTAACAAAAA TGTGCTGGAG 
ACACAAGCCA TTGCCCTCAC TAACAAACAG AACAAATGTG ATACAGTGTT GTTTTTTGAT 
GAAGCAAACA CAACTCAAGC CATAAGCAGT ATCAAAGAAG TCCTGTGTGA CCACACTGTG 
GATGGCTTGC CTTTAATTGA AAACTCAGGT TTGCAAGTCA TTGCTGCATG TAACCCCTAC 
AGGAGGCATA CAGAAGAAAT GATCAGACGA TTGGAATCTG CTGGTTTAGG GTACAGAGTA 
AAAGCAGATG AAACTAATGA ACGACTTGGG TCCATCCCAT TAAGACAATT AGTCTATCGT 
GTTCATCCAT TGCCTCCAAG TATAATGCCA CTGGTTTGGG ATTTTGGTCA ACTAAATAAT 
GAAACCGAAA AGAAATACAT TCAACAGATA GTACAGCATT TGGCAAATGA GATTGAAATG 
CATCAGAGCG ATACACAGAT ATTGACCAAT GTGCTTTCAG CTTCCCAGTC ATATATGAGA 
AATAGAAATG ACGAATGCAG CTTTGTAAGC CTTCGAGATG TTGAACGTTG TGTAGAAGTG 
TTCAGGTGGT TCTACCGCAA TAGTGACCTC TTGCAGGCAA ATATAGAAAA TAAAAAGGCG 
TGGTCTCTTG TTTTGGCCAT TGGGGTATGT TACCATGCCT CATTAGAAAA GAAAGATGCA 
TACAGAAAGG CAATTTGCCA ATATTTTCCT GAACCCTACA GAAACCAAAG AATAATTCTT 
GAGAAAATAA CAAGTATCCA AGACCTCTTT CTAGATCGTA TACAAATAAG AGACAACATT 
GCCAGGAATC TTGCTTTGAA GGAAAATCTA TTCATGATGG TGATTTGCAT TGAGCTCAGA 
ATTCCATTAT TTCTTGTTGG AAAGCCTGGA AGCTCAAAAT CCCTTTCAAA GACCATTGTT 
GCCGATGCAA TGCAAGGCCA AGCATCACAC ACAGAACTCT ACAAACATCT TAAGCAAATA 
CACCTTGTAT CTTTTCAGTG TAGTCCTCAC TCAACTCCAG AAGGCATCAT TGGCACTTTC 
AGGCACTGTG CTCGCTTCCA GAAAGACAAG AACTTAAATG AGTATGCTTC TGTGGTAGTG 
CTAGATGAAA TAGGGCTGGC AGAAGACTCT CCTAAAATGC CTTTAAAAAC ATTGCACCCT 
TTGCTGGAGG ATGGTTGTAT AGATGATGAG ACATCACCTT ACAAAAAAGT TGGCTTCATA 
GGAATTTCTA ACTGGGCGCT AGATCCTGCA AAAATGAATA GAGGCTTATT TGTATCCCGT 
GGAGACCCTG ATCAAAATGA GCTTATTGAA AGTGCAAAGG GAATCTGCTC GTCTGATCTC 
TTGACCATTA AGGAAATTGA CCATCATTTC AAAAGTTTTT CCGATGCTTA TCTGAAACTG 
TGTGAAAAAC AAGATAAAGA ATTCTTTGGT TTACGAGATT TTTACAGTTT GATAAAAATG 
GTGTTTACCT ATGCGAAACA CTTAAAAAAT GAGCCAACAC AGCTTGAGAT TGCAACAGCA 
ATACTGAGGA ATTTCAGTGG GAAGGATGAC TTGGATGCCT TGAGTATTTT TATAACAAAT 
CAAGAAGCAA ACACATATAG GGAGGAAATT AAAATAATGG ATTTGGTAAT GGAGAACATA 
AGAAATGAGA GTGCTGATAT AGAGGCTCGA TACCTGTTGT TATTAACTAG AAATTACGCA 
GCACTGCAGA TTCTACAACA GACACTGGCT AAAGAAAATC AACATCCTGA GATTATATTT 
GGGTCCAGCT TTCCAAAAGA CCAAGAATAT ACTCAGATCT GCAGAAACAT CAACCGTGTT 
AAAATCTGTA TGGAAACAGG ACAGATGGTT GTCCTTCTTA ATCTCCAAAA TCTTTATGAG 
AGCCTCTATG ATGCATTAAA TCAATATTAT GTCTATCTAG CTGGACAAAA ATATGTTGAC 
TTGGGTTTAG GCACACATCG GGTAAAATGT AGAGTCCACC CAAAGTTTAG ACTGATAGTC 
ATTGAAGAGA AAGAAATTGT GTACAGAGAT TTTCCAATTC CTCTTATTAA CAGACTTGAG 
AAACATTATC TGGATCTAAA CACAGTTCTA GAGAGGGGAC AAAGAGCCGC TGTAGAGGAA 
TTGAATGACT GGGTTAAAGA TTTCACTGCA GGCGACAGAG AACAACTAAT TGGCCAGAAA 
CAGGACTATA AACCTGCAGA TGTCCTTATT GGTTACCATT CTGACACCTG TGCATCAGTG 
TTGTTACAAA TAACAGGAAA ACTGAAAGAG GAATGTGGTG ACAGTGACCT TTACATGGCA 
GAAGTGAAAG AGGAGGCAAA GGCTACTCTG CTGAACTGTG CAACACCCGA TGCTGTCATT 
CGACTGGGCA GAAAAGAGTT GGTCGATGAG TACTTCAACA AACAGAAACA TGGATCTCTG 
GTTGACTTTG TTAAGAGCCA CATCCATTCA GGATCTAAAC ATCAAATTGC ATTTACAGAG 
ATCACTACAT TTTCACGCCT TTTGATGTCT ATTGACAAAA GAATTTTGGA AGCCGAGACA 
AAGGATGATG TCAAAAGTGT AGAGGTGCTG TCATTGCAAC AGTTTGATAC GGAACATTCT 
TTCCTGAAAA AAATCAGGAG TTTTCTAAAA GATTCCACTG GTAATAAAAT ACTGATCATA 
CAGTCAGACT TTGAAGATGG ATCTCAAGGA GCCCATTTGG TTGCATCTGC CAAGTACGCC 
ACTGTAAATG AAATAAACAA CTTAAAGAAA GACAACACTG AGAAGACATC AACCTTTGTC 
TATTTTATAA CTAAACTTCC TAGAATACAA GGGGGGTCAT CATATGTGGG ATTCCATGGA 
GGACTTTGGC AATCTGTTCA TATTGATGAC TTAAAGGAAC CAAGCGATAA GTTGCCAGAT 
GTAACTGCCC TTCAAAACAT GACAATAAGC CAATTATTTT ATGGAGCCAC AGCTGAATCA 
CAGTTAAGAT ATGTTGATGG CATGGAAGGT GGCACAGACG CTAGTTATGA CACTGAGCCA 
GACCAGATAC CAGAGGTAGT AAACACCACC TTTCTCATTC GGAGCTGCAT ACAGAGTGCA 
GTGGGCATGA TTACAGATGA AGAGAATACT CCCAGCCGCA GCACAAGGAG GATTGAAATT 
CTAGTTAATC TACTGAGCAC CGAGGACAAT CTAAGAGCTT CCCTTCTACA AACACTGAAA 
GTTCGTGTTT ATGAAATTCT GAAGAACCAG GAAGAGAATT CATATAACCC AAATGAGTGG 
CTTGTAAGAG CTGCCTCCAA CCTTGATGCA TTGCAAGAAG CTGGCACATT CCGATACACT 
CTTTGGAAAC GTATTCAGGC TGCTGTGATC CCAATATTTG CACATCTCAT TTATCTGATG 
GATTGTGATG CCAACCTGGA GATTCTGGTG AACCCAGAAA CTGAGGACTT TCTGAAATCT 
TTATGGATTT ACATTTTCAA AAATAAAAAT CTTCTAAATA TTTCCAGCAG CCTTGGTAAT 
AATAGTTCTC AGACCATAAA GGCAACTAAT TACATGAACC TGTTGTTTTG TGAGGGGAAT 
TCAGTTCCGT TCAGCTGGAG AATTAAAGAT TATTTGGAAG AAATTACAGC ACAAGCTCAG 
TACATTGATA GTGTAGAAGG TCCAGAGATG AAGTTTGTAA ATATGTTTAG CAATGTGCCT 
TTGGGACAAT ATATAAATAA CATGGAGGAA AATGATCAAC AAAAGCTGTT CCAGTACTAC 
AAACGGGACT TTTTACTTCT TACTGTGAAT ATATCCTCCA GGGATGAACT CAAGTTTTTA 
GAGATTTCGC TTTCCGCTTG TATTGAAGAA TTTAGAAGAA GTAAAGAAGG AGAAGAGCTG 
ACACTTCCCT GGGTGCATCT CGCTTATCAC CATTTTCAGC ACAGGTTCCA AATATTCTCT 
AGGATTGTAG CGATCAGTCC AACTGTTCTG GAGAGGATGA ATGAAGGTTT CCAAGAGATA 
GAAGAGATTC TAACAAATCA GATGGCTATT GATATATGGG CTGGATTTGA ATGCTTAGAC 
ATTTTGGAAG CCACCATTCT GGACCCAAAC CCACAAGCAT GGTTGAATGA AGTAAAAAAC 
CTTCAGCTGA CAATTGAACT CATTTGCTCA GAAAAATACT TAGAAGGGGA AAGTGACTGG 
TGCACACAAA CAGTTGAGCA GATCCGAACC ATGTGGCATT GTATCTACGC TATTGCATTA 
TTTACAGAAC ATGTCATTTT GGACGAGACT CTGCAGAACC CTGAGACCCA ACAAAAACTA 
AAAGAGCAAA CTATTATGTT TGGCCAGCAT ATCAAAGGCT TTCCTGAAAT AAAGTCAATT 
GATCCTTATA AGGCAGTGAT TGAAGTTATT AAGATGAGCA GAGAGGCAGT AGCTGAATCA 
CTTTCCAGGG AAGACAATGT TCAGTTCCAG CAAAACTGCA ATGGATTCTT CATTGACATT 
CTATGTACTC GGTTCTTTGA AGGCAACTCT CCCCCAGACA AAGAAGTTGT TGATCAGCTG 
CTTTCTTCTC TTTTCATAAC TGGTTCAGAG AATGATGGAA ACGATCTGTC ACCTTTTACT 
GCTGGAACTA ATGAAAACCC TGCTATCCGA TCTGTGATCC TGAAGATGCT CCTCAAGTAC 
AGCTTTGAAG AAACACAGAA ATATATCCAG GATCACTTAA TCAGAATACA AGGAAGCAAC 
TTGACCACAG AAATACACAT GCTCTTTATC AACTGCCTTG AGGACTGTAT GTATGAAAGG 
TTTCAAGCTC TGCGTGAGGA AGACAAATTG CATTACCTCA AAGACCAAGG TGAATTTCTG 
AGAAACTTCC TCCAAAATGG TCATCCCCCT AATTCCCAAG AGACCATGGT TGGGTTCCTG 
CAAGATCTTG CCAAGGTTAG GTTTGCACTC AATGCAGTAG CGGAACTGCT CTCTGAACCA 
GACAATGTTG TAAATGAGAA GAGGGAATTT CTCCAAAGTG GACAACATTT TTGTGAACAT 
TCAGCTAATG ACTGGTACCG AGTGTATTTG ATCCGCAAGA TGGGAAATGA ATCCGGAAGT 
GAATATGTCC AAAATCTTAG TAAAGATGAA GAGTTTAGAT GGTTATTCCC ACATCAAATC 
TTTGGGGAAA GGGAGAACAA CGCTAACCAG CTTGACCGAT TTTTGGTGCA TAAGGATGGT 
TATAGGGAGT TAAGAGAAGG TGTTGGAGAA GTCATAGTTT CAAGAAAGGA GGAGAGTCTT 
CAACAAAGAC TAGAGTTGGA TGAAAGTTCT GCCAAGGAAC AAGCTGTTTA CCTGCTCCTC 
GCCATTTTCT CTGAAGTAAC AATGAGATAC AGAGAAGCAA ACAGGGTCGT CACTCAAGAA 
GAGACTGATA ATTTAAGGAA TTTCATTCAA AATACCAATG TTCTCCAGGA GCAGTCAATT 
CAGCAATTCT CCCAGTCACT CCTAACAAAC TGCCGACCCC TAGTGAGAGT TGCTCCAGAC 
ATGACAAGTT CTCATTACAC TATAGTGTCC CTGGCCGTAC ATCTTGGTGC TGTGCTTCTG 
AGCACTGATA ATGATCTGTT TCAACCACTG AGGAATTTAG CCTTTTCCCC TGACAGAATG 
CAGCAATCAT TCCTACCAAC AATGCCACAG GACATGCTAA ACAAAGCTAG AGAAGTATTG 
GGAAATGAAA CTTGGTATGT CTGTGACAAC AATCATTATG TTCCAGTTCC TGGCTGTGGG 
ATGCCAATGA GCTACGCCAA GTGCCTAGAA TGTGGAATTG AACTGGGGGG AGTTGACCAC 
AAAACTTATA AAGGAATGAG AAAGCTTGAA CAGGACATTG ATGAGACAGG ACATATTTTG 
GGAGCACCTG AGACTCCAGT GTCAGTTGCG GCAGAAGACA GAGGCATGAG TGCTCCTGTA 
TTTAATGTAT TGAGGCTGGT GACCCACCTG GCGATGTTAC TAGGGGCAGA ACAAAATCGA 
GAGAATGTGA AGACCATGGT GAAACCAGGA GTGGAAGATA TTGGGACTTT CCTTTTCCGA 
CATCTAGAGA AAAATTTGGA ACAAGTAAAG AACTCCCTAG GGGAAAGTGT GGATAATACG 
GCCACTGTAC TCCATCTTGT TTTCTGCAGG ATGCTCACTA TTTCCAATGA TCAACCTGAC 
CAGCCTCTTT TACCTTTCAA TCATAATCTG TCAACAAAGG ACGTGAGAAG CAACTGGGAG 
AGAAATATGG CGGCATCAGT CATCGCTCCG GTGCTTACGA ATTTAAACCA AGATCTTGAT 
GATGTCAATA ACTACATAAG GAATGACAAG AGAATAAGTT CTGACCCAAT AATCAGAATC 
ATATATGGGG AACCGTTGCA TAACACTGAT ATTGTGGCAT TGCCAGAAGA TGGACTCATT 
CCCTACTCAA AAATATGGAG CTGCAGGACA AGAATTACTG TTGGGCATCT TCAGCATATG 
GTTGAGCAGA AGGATGGGGT GAATAATCTG CCGATGCTGT CAACATTCCT GAAGGATGAA 
TGTGAACTCC GCCTGGTTAA GTTCCTACCA GAAATATTAA ATCTTCAGAA AGAGCTGGTA 
AAAAGATTCC AGAACTTCCG AGATGTGAAC AAACAAACAA TTGGGGAATT TTTAACCAGC 
ATACCTGTTG ATGGTGCTCG ACGTGTATTT GAGAAGAGAA TACAAACTTT CCTATTTACA 
TGGAATTGTC TAAGACATTC TCTTCTTATG AAAGGGGAAA TAAAACTCCC AGAAGAAGCC 
TGTGCCAGTG ACCTTACCCT AGCGAGTGAC TTTGCCATTC TCCTTCCGAG TCGACAAGGA 
CAGGGTCTCT GTGCCACAGC ATTGGTCAGC TATCTCATCG CTCTGCACAA CAGCCTGGTT 
TATGCTGTGA GACGCTTCAC CAGAGATGAG CAGACGTATT CTATTGGGGC AGCTGAAGCC 
ATGGATCTAC ATGTGATCAG TTATGAGGTG GAAACAGATC TGATGCCAAT AATTCTGTCC 
AACTGTCAAT ACAACACCCA AAGAGATGGG AAAGCCTTAG AGGAGATTGA TCTGCAAAGG 
ATCCAGCGTC AGATCATCAG CCGATTTCTC CAAGGCAAAC CTCGCATTAC CCTTGTAGGG 
CTGCCTACCA TTGTCCACTC CCAAGATCGG AACTACGAGA ACATATTTAG AGACGTGAGG 
GAAAAAGTCT TACAGGATCC ACTGCCAAAT TCAGCAATAG AAAACATAAG CAAAGATCTG 
GATTTGTACA GCGATGTCTG TGATGCGCTT CAAATCATTG AGACTATGTT GGGGTTCTTA 
GCAACATCCG GGGGCAATCC AGATCTTCCG CTGACCACTT ATGTAGAAGA TGTCCTACAG 
ATGAAGGATC AGAGCGATGA GAACATCCTG GAGGCATTGA AGCAGTGTCA CTTGAAGCAC 
ACAATCTCAC TATGGCAGCT TCTCACCACT TTACAGTCTG AACACCGCAT ACGCCTTAAA 
AATGATCCAT TTGCAGATCT GGATGAAGGG TATAAGCAGG AATTAGATGA GGAGAGTCAG 
AAGCTACTGA CTGCGTTCTT GGCACAACAT GGACTCAACG TCTTTCTTCT GGAATTACAT 
GAAATGATCA TCTTAAAGCT CAAGAAGCAA CATGGGGCCA ATGATTTCCA ACCTGCATGG 
GGGTTAAGAG AAGTTCTGGG CCCATTGCTT GATGGGAAAG AGCAACCATT CCCGGCGCTG 
GAGGAGGATT TCCCAGAGCA GATCTGTTTG GCTCACTGTG TGGAGAGCTG GAAAGTGGCG 
GCCGCCATGA AGTGGAAACG ACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108699444 ( XM_018231529.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018087018.1 Xenopus laevis E3 ubiquitin-protein ligase RNF213-like (LOC108699444), transcript variant X3, mRNA.

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CloneID	OAf43054
Clone ID Related Accession (Same CDS sequence)	XM_018231529.1
Accession Version	XM_018231529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 13143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase RNF213-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAC TCTACCAACA TCTTATCAAC AAGAATTTAT CAGCAGTGGA TGCCATTATA 
AAAGACAGGC TGATGGCTGG ATTACTCTGC GCCAAACTGA TAGAAACCTA CTCACTTCCT 
GCTGACAAGG ATTCTCTTGT AAAGGTTTGC AGACTCCTCT CTTTGGAGCA GATGCTTAAC 
AAAAACCTAC CCATCCAACT TGAGGAACTG AAAAGAATCT TCTCAGTGTA CGAGGGAGAT 
GTCCAAAAAT ACATGAGGAG TAAGGAGAGT TTACAACAGG CAGGCAGAAC TTTACACAGA 
TCTTGGCTGT GTCTATTACC ACTGGAAAAC TTGTTCACGT TTTTGGATAC AATTCCCACC 
GATTTCCGAG ATGCTTTTAC CGCTGCCTGG TACAAATTTC ATACACAATC CTCAAGCAGC 
AAAGATAAGC ACATTGAGAA GATTCTAACA AAACTTCTGA ATATGGCAAA AGGACCGAGC 
CAGGGGTCAC TGCAGAAGGA CTCCATATAC CTGTGTAATA TCAGTTTGAA ACTTCATCAT 
TTAATCTGCA CTCAAACAGG GCTGAATAAA AACAGTCAGC TCCCACTTTT GTCTTTCAAG 
ATCGCACTAA AGCTTTTTGG AGCAGCACAG AAACAGGAGC CAATGAAAGG GAGGGAGACC 
AGTATCAATG AAGTGCAAAG CAATTTAAAC AAAGCTGTTG AGATGCTGAA GACATGGCTA 
CAGGACTTCT TTGGACATAG ATTTAATTTT AGACGCTATG GATATCCAAC ATTTGGGGAT 
GAACTTGAGT TTTGGAATAA CCTTCTTATG AATGACGACA ATATGGACCT GAAGTGGAAA 
GAACTGCTTC TCTCAGATCT GGCCAGGAAA ATAAAAGAGG AAAGCCCTGT GGAACAGATT 
AAAATCTTTT GCAGAGAACA TTCACTGTTG CAACGGCTTC ATATCTCCAT AGAAGAATGC 
TTTGAGGAAT GCGCCATTAA TGCTGTTCAT AATGCATATA AGTCTCATAA CAATATTCTC 
AACCAAATCT CAATAAACAA TCTACAACAT TTTAGAAAAC TGGTATCAAC AATAATAAGA 
AACTCTTGGC CAAAGGATGT GCAGGGAAAT CCAACTGAGA ATAATAATGA GATTTTAAAG 
CACATGCTGC ACTGGAAGGA TTTCACTCAT ATATTCAAAC TATATGATCC TAACCAACCA 
ATCACCTCTC AGTTTATAGA AGAAACTCAA AAGCTTGTTA CTTTGTCAGA TTCGGTCCTT 
CTTGACACCG TTTGTCGCCT AATGCATGGA GATATTCTGT ATGAACACCT CAAGTTGGTA 
CTGGGCAATA AAAACCAGTT CTTGGATATA TGTAGACTGA GAGAAACCAT AAAGGTGGAA 
GATGTTAAGA AAGTTCTGGA GTGGAGGAAT GAAGAGCTTA AAACAGTGAA CTGTAAGAAA 
ACATGGGTGG AGAACTTGCT GAAAACGTGC CAGAACATCA GCAAGTCTGT CCGAGTGAAT 
ACTGAGGAAA TTCAACAAAT CCTTTCTGTA AATCTGGGAG CACAAAAGTT AAATGATTTA 
TTACCTGTTA AGACACTTGG TGCTACTGAA CCTCCTGAAG ACAAGGTGAC ATTGTTTAAC 
AGTTTCACTA ATTCAATAAT GTCAATGGCC GAGAGTCTTC ACACCCACCG GCACAGTCAT 
GTTTTCCGCA TGTTGTGGAA TGAGGCTGTT AGCTCTCTTT CAAAGAGAGA TGAAAATTTA 
GAAAATGAAG AAGATGATGA ACTTACTTTG GATGAGATGC AGGAATCCTT GTTCACACCA 
TGTTTTGATA AATACACCAA GATATACGAG GACATCAGAT CTGAAGAAGT GACCTTTGAA 
GTCCTGGACA CATATTTCAA GACTTTCAAA AACCATTACC AAGCCCTACA TAAAGAACTA 
CAAACCATAC GCCAGTTAGA TCCAGAGGAT AATGGTTCCT GGGTCAGTGG CCGAATAAGG 
AAAATTGAGC AGTATCATCA GTTACGTGAA GCCTTTGAAT CAGCCCAGAT TGTTGATGAC 
ATCAGAGATT TCTGTAAACT TTTGGGAGAT TTTAAGACCC TGAATACTCT GCAACAATTT 
GGTGCTGACT TTAGTGATTA CAAGGAAAAA CCTTTATCTT TTATCACTGA AGACATTGTA 
AAGACAAAGG AAGCGCTTTG TGAGATAACA CAAGAACGTG CTGAGGCTCT GAAGGAGGCT 
TTAATGAGAA AGGAATTTAT AAAGTGGGTT CGAAACTCAC TGGAAGATGT CAATGAACTG 
AAAGTTTTTG TGGATCTGGC CTCCATTTCG GCTGGTGAGA ATGACATGGA TGTTGACCGT 
GTTGCTCAGT TCCATGATGC TGTTTTGGGC TACGCCCCTA TTTTGTATGA ACTCAAAGAG 
GAATTTGGAT TTAATGAATT AATGTTATGC TTGAAAAAAC TTTGGAAAGC CCTGGAAAGT 
GACCCAAATC TGCAGAAAAA ACTGAGAGAC TCTGCCCGTC ACATAGAGTG GCTGAAAACT 
GTAAAAGAAA GTCATGGATC TGTGGAACTC TCATCACTGT CTCTGGCATC TGTTATAAAC 
CAAAGAGGCA TCTACATTAT TCAAGAGCCC AATGAGAACC TAAAGATAAA CCCAGACAGT 
GTCCTGAGAC TAGAAATTCC TGATGATGAT CTGGAACAGA ACCAGGTGCG CACCTTTAAT 
TTAGAAGAAC TGAAGGAGCT GTTGAGCAAG CTCATGCTTA TGTCTGGGAA AGGTGACCAG 
GGTAATGCTG AAGTTGAAAA GTTTTCAGAG ATTTTCTCCA ATGTTCAGAG ACTTGCCAAG 
GCCTACATTG ATCTCTGCTT GTCTGGAGAC ATTTTATTCA AAACTTGGGA GGCCACTATC 
TACTGCTCAG ATGAGATTGA TATTGCTATT AGGATGAAAT TTAAAGTAAC AGTAACAGCC 
ATTGGGGAGA TAGGAGCAAC AGGAAATATT TTTGAACTGC TTCCCAAAAT CTGCCAAACC 
ATGGAAGACA TATTAGAAAA AAGGCAAACT TTTATGGATG AAAAACGTTC TCAGCATTAC 
TTTCTAAATT ACTACACCGC AGAGCAGTTG GTCTTCCTTA GCCAGCAGAT TCAGAAGCCT 
GATGTTAGTG AAGAGCTGCT TGTTATGTTA TCCTTTATCA AGCCTGAATG TACAAAATCT 
GACACCCAGC AAGCTTTATT AGCTGCACGG AGGAAAGGAA CATCAAAGCT CCTTGCGACT 
ACTAATAATT CTGCTTTGTT TAACTTTAAA AAATCTACCA ATGTTCAAGA GAAACTACAG 
ATTGCTTGGA AATACTCCAT GGACTGCATG AACAATTTGT TTCCTGGATG TCTGGATATA 
AATACACTTG GAAGGTCCTT AACTGAACTG GCAAAAAAGG AAAATAGCCC AGTGACCAGA 
AGTCTACATC CCAGTCTAAA ACCGGGATGT CCCAATTTGG TACTCTGTGC ATCATCAGAG 
ATTCTATCTT CAGCTCTTGC TGTATATATG CAGAATTTAA ATCAGCCACT TCCATCTTAT 
GATGAAGTTC TTCTCTGTTC ACCTAAAACT ACTTACGAGG AGGTGGCATT GTTTTTGAGA 
AGATGTTTCA CACCAGGCTA CAGTGGGAAG AAGATTTACA GTTTGCTGTA TGCAGAAGAT 
CTCAGCTATG ATACAGCCTA TAGAGCTGAA CAACTGTTTT ACCAACTGCA AAAGACAAAA 
GGCCTAGACT ACCACCTTGT GATTATCTGT GACCGTGAGA GACATCATTT GCATATACCA 
TCTACCTTTA GCCAATACAA AGTGCAAGTT GTTCCTCAGA GACTTTTTGT TGAAGTTCAA 
AAATATTTAT TGCAACATTT TACTATTGAT CCAAATGTAG TCTCAGCTGC CTCCGTTTTT 
AAAGGCGTGA GTGTGGGAAT AGTGACCTCC AAACGAGCAG GAGTTGGAAA GTCGCTCTAT 
GTGAAGAGAC TATATGAAAG GCTGAAACTG GAATTTCCAA ATGAAAAGCC TCTCCAAAAA 
ATAATCCGAA TGTTTCAGCC CCAGGTGGAC GAAAACAAAG TTCTGCAAAA GCTTCTTCCA 
TTTCTAACCA AGGAAAATAA AAATGTGCCC ATAATATTTC ATATTGATAT CACCTCTTCT 
GTTAACAGGG GAATTTCAGA ATTTCTTTTC AAACTTTTGA TTCTTCAATA TCTAAAAGAT 
GATGAAGGGA GAATGTGGAA GCGCCAGTCT TGTCAGCTTT ACCTTATTGA AATCTTAGAA 
TCTTCTTGCA TAATTTCTGA GAAGCAGAAA AAACCTGCAC CTCAGATGGC GCAAAATATT 
TTTATGGACT TTTTCCCTAA AATATCATGC TGTTCTCCAA ATGAGGTGCT GACAAAAGTC 
ACAGAAAATC AGACTGGACA AAATGAAGAT CCTGGAATGG ATAGAAAGGA ATTTGAGAGT 
GAATGCTTTC AGCGGCCTTT TCAGTACTTG ATACGTTTTG AAGGGAAACA AAGTCTGGAT 
AAATTTACAT ACAAGGAAGG TTCCGTAGAA GGAAATGAGG CAAAATGTCT GCAGTTGTTC 
CTTTTGCACT GTGGGGTTAT GGACCCATCA TGGGCAGAAC TTCGAAATTT TGCATGGTTC 
TTAAATGTTC AGTTAAAAGA CAGTGAGAAT TCTGTTTTCT GCAAACCAGA ATTTGTGGAA 
GATGCTATGC AGGGCTTCAA GAATTTTGTT GTAGACTTTA TGATATTGAT GGCAAAAGAT 
TTTGCAACTC CTTCATTACA GATCACTGAT GAGAGTCCAG GCAGACAAAC TTGTGAAGAA 
GTCTGTACTA ATGATGGAGA CCTTGCTCCA TTTCAGATAC GTAAAAAATG GGAGGCTGAG 
CCACATCCCT ACATTTTCTT CAATGATGAT CGCATGACAA TAACATTTAT CGGCTTTCAC 
TTGTGCGTGA ACAGTGCTGG AAATGTTGAT GCGATCAATC CTAGAGATAA GAGCATTATT 
AAGAAAAATA TTATGACAAG ACAACTTTAT CGAGACTTAA TGCTTCAGAA AGTTCAGTTC 
GATATAGACT TTGACACTCT ACCTAGGGAG GAAAAGATTA ATATGTTATC CAGAGTTTTG 
GGGATTAATA AACCGCAGGA TCCAGATGAA ACATATGAGT TGACTATGGA TAACACTCTG 
AAGATCCTGG CTATTACAAT GAGGTTCCGG TGTGGAATTC CTGTTGTCAT TATGGGAGAA 
ACAGGTTGTG GAAAAACAAG ACTCATAAAG TTTATTTGTA AATTATGGAA AGGAAACCTG 
AATGCAGAAA ATACTACATC TGATGCAGAA AATATGAAAC TTGTAAAGGT TCATGGCGGT 
ACAAGTGCTG AGAGTATTAA CAAAAATGTG CTGGAGACAC AAGCCATTGC CCTCACTAAC 
AAACAGAACA AATGTGATAC AGTGTTGTTT TTTGATGAAG CAAACACAAC TCAAGCCATA 
AGCAGTATCA AAGAAGTCCT GTGTGACCAC ACTGTGGATG GCTTGCCTTT AATTGAAAAC 
TCAGGTTTGC AAGTCATTGC TGCATGTAAC CCCTACAGGA GGCATACAGA AGAAATGATC 
AGACGATTGG AATCTGCTGG TTTAGGGTAC AGAGTAAAAG CAGATGAAAC TAATGAACGA 
CTTGGGTCCA TCCCATTAAG ACAATTAGTC TATCGTGTTC ATCCATTGCC TCCAAGTATA 
ATGCCACTGG TTTGGGATTT TGGTCAACTA AATAATGAAA CCGAAAAGAA ATACATTCAA 
CAGATAGTAC AGCATTTGGC AAATGAGATT GAAATGCATC AGAGCGATAC ACAGATATTG 
ACCAATGTGC TTTCAGCTTC CCAGTCATAT ATGAGAAATA GAAATGACGA ATGCAGCTTT 
GTAAGCCTTC GAGATGTTGA ACGTTGTGTA GAAGTGTTCA GGTGGTTCTA CCGCAATAGT 
GACCTCTTGC AGGCAAATAT AGAAAATAAA AAGGCGTGGT CTCTTGTTTT GGCCATTGGG 
GTATGTTACC ATGCCTCATT AGAAAAGAAA GATGCATACA GAAAGGCAAT TTGCCAATAT 
TTTCCTGAAC CCTACAGAAA CCAAAGAATA ATTCTTGAGA AAATAACAAG TATCCAAGAC 
CTCTTTCTAG ATCGTATACA AATAAGAGAC AACATTGCCA GGAATCTTGC TTTGAAGGAA 
AATCTATTCA TGATGGTGAT TTGCATTGAG CTCAGAATTC CATTATTTCT TGTTGGAAAG 
CCTGGAAGCT CAAAATCCCT TTCAAAGACC ATTGTTGCCG ATGCAATGCA AGGCCAAGCA 
TCACACACAG AACTCTACAA ACATCTTAAG CAAATACACC TTGTATCTTT TCAGTGTAGT 
CCTCACTCAA CTCCAGAAGG CATCATTGGC ACTTTCAGGC ACTGTGCTCG CTTCCAGAAA 
GACAAGAACT TAAATGAGTA TGCTTCTGTG GTAGTGCTAG ATGAAATAGG GCTGGCAGAA 
GACTCTCCTA AAATGCCTTT AAAAACATTG CACCCTTTGC TGGAGGATGG TTGTATAGAT 
GATGAGACAT CACCTTACAA AAAAGTTGGC TTCATAGGAA TTTCTAACTG GGCGCTAGAT 
CCTGCAAAAA TGAATAGAGG CTTATTTGTA TCCCGTGGAG ACCCTGATCA AAATGAGCTT 
ATTGAAAGTG CAAAGGGAAT CTGCTCGTCT GATCTCTTGA CCATTAAGGA AATTGACCAT 
CATTTCAAAA GTTTTTCCGA TGCTTATCTG AAACTGTGTG AAAAACAAGA TAAAGAATTC 
TTTGGTTTAC GAGATTTTTA CAGTTTGATA AAAATGGTGT TTACCTATGC GAAACACTTA 
AAAAATGAGC CAACACAGCT TGAGATTGCA ACAGCAATAC TGAGGAATTT CAGTGGGAAG 
GATGACTTGG ATGCCTTGAG TATTTTTATA ACAAATCAAG AAGCAAACAC ATATAGGGAG 
GAAATTAAAA TAATGGATTT GGTAATGGAG AACATAAGAA ATGAGAGTGC TGATATAGAG 
GCTCGATACC TGTTGTTATT AACTAGAAAT TACGCAGCAC TGCAGATTCT ACAACAGACA 
CTGGCTAAAG AAAATCAACA TCCTGAGATT ATATTTGGGT CCAGCTTTCC AAAAGACCAA 
GAATATACTC AGATCTGCAG AAACATCAAC CGTGTTAAAA TCTGTATGGA AACAGGACAG 
ATGGTTGTCC TTCTTAATCT CCAAAATCTT TATGAGAGCC TCTATGATGC ATTAAATCAA 
TATTATGTCT ATCTAGCTGG ACAAAAATAT GTTGACTTGG GTTTAGGCAC ACATCGGGTA 
AAATGTAGAG TCCACCCAAA GTTTAGACTG ATAGTCATTG AAGAGAAAGA AATTGTGTAC 
AGAGATTTTC CAATTCCTCT TATTAACAGA CTTGAGAAAC ATTATCTGGA TCTAAACACA 
GTTCTAGAGA GGGGACAAAG AGCCGCTGTA GAGGAATTGA ATGACTGGGT TAAAGATTTC 
ACTGCAGGCG ACAGAGAACA ACTAATTGGC CAGAAACAGG ACTATAAACC TGCAGATGTC 
CTTATTGGTT ACCATTCTGA CACCTGTGCA TCAGTGTTGT TACAAATAAC AGGAAAACTG 
AAAGAGGAAT GTGGTGACAG TGACCTTTAC ATGGCAGAAG TGAAAGAGGA GGCAAAGGCT 
ACTCTGCTGA ACTGTGCAAC ACCCGATGCT GTCATTCGAC TGGGCAGAAA AGAGTTGGTC 
GATGAGTACT TCAACAAACA GAAACATGGA TCTCTGGTTG ACTTTGTTAA GAGCCACATC 
CATTCAGGAT CTAAACATCA AATTGCATTT ACAGAGATCA CTACATTTTC ACGCCTTTTG 
ATGTCTATTG ACAAAAGAAT TTTGGAAGCC GAGACAAAGG ATGATGTCAA AAGTGTAGAG 
GTGCTGTCAT TGCAACAGTT TGATACGGAA CATTCTTTCC TGAAAAAAAT CAGGAGTTTT 
CTAAAAGATT CCACTGGTAA TAAAATACTG ATCATACAGT CAGACTTTGA AGATGGATCT 
CAAGGAGCCC ATTTGGTTGC ATCTGCCAAG TACGCCACTG TAAATGAAAT AAACAACTTA 
AAGAAAGACA ACACTGAGAA GACATCAACC TTTGTCTATT TTATAACTAA ACTTCCTAGA 
ATACAAGGGG GGTCATCATA TGTGGGATTC CATGGAGGAC TTTGGCAATC TGTTCATATT 
GATGACTTAA AGGAACCAAG CGATAAGTTG CCAGATGTAA CTGCCCTTCA AAACATGACA 
ATAAGCCAAT TATTTTATGG AGCCACAGCT GAATCACAGT TAAGATATGT TGATGGCATG 
GAAGGTGGCA CAGACGCTAG TTATGACACT GAGCCAGACC AGATACCAGA GGTAGTAAAC 
ACCACCTTTC TCATTCGGAG CTGCATACAG AGTGCAGTGG GCATGATTAC AGATGAAGAG 
AATACTCCCA GCCGCAGCAC AAGGAGGATT GAAATTCTAG TTAATCTACT GAGCACCGAG 
GACAATCTAA GAGCTTCCCT TCTACAAACA CTGAAAGTTC GTGTTTATGA AATTCTGAAG 
AACCAGGAAG AGAATTCATA TAACCCAAAT GAGTGGCTTG TAAGAGCTGC CTCCAACCTT 
GATGCATTGC AAGAAGCTGG CACATTCCGA TACACTCTTT GGAAACGTAT TCAGGCTGCT 
GTGATCCCAA TATTTGCACA TCTCATTTAT CTGATGGATT GTGATGCCAA CCTGGAGATT 
CTGGTGAACC CAGAAACTGA GGACTTTCTG AAATCTTTAT GGATTTACAT TTTCAAAAAT 
AAAAATCTTC TAAATATTTC CAGCAGCCTT GGTAATAATA GTTCTCAGAC CATAAAGGCA 
ACTAATTACA TGAACCTGTT GTTTTGTGAG GGGAATTCAG TTCCGTTCAG CTGGAGAATT 
AAAGATTATT TGGAAGAAAT TACAGCACAA GCTCAGTACA TTGATAGTGT AGAAGGTCCA 
GAGATGAAGT TTGTAAATAT GTTTAGCAAT GTGCCTTTGG GACAATATAT AAATAACATG 
GAGGAAAATG ATCAACAAAA GCTGTTCCAG TACTACAAAC GGGACTTTTT ACTTCTTACT 
GTGAATATAT CCTCCAGGGA TGAACTCAAG TTTTTAGAGA TTTCGCTTTC CGCTTGTATT 
GAAGAATTTA GAAGAAGTAA AGAAGGAGAA GAGCTGACAC TTCCCTGGGT GCATCTCGCT 
TATCACCATT TTCAGCACAG GTTCCAAATA TTCTCTAGGA TTGTAGCGAT CAGTCCAACT 
GTTCTGGAGA GGATGAATGA AGGTTTCCAA GAGATAGAAG AGATTCTAAC AAATCAGATG 
GCTATTGATA TATGGGCTGG ATTTGAATGC TTAGACATTT TGGAAGCCAC CATTCTGGAC 
CCAAACCCAC AAGCATGGTT GAATGAAGTA AAAAACCTTC AGCTGACAAT TGAACTCATT 
TGCTCAGAAA AATACTTAGA AGGGGAAAGT GACTGGTGCA CACAAACAGT TGAGCAGATC 
CGAACCATGT GGCATTGTAT CTACGCTATT GCATTATTTA CAGAACATGT CATTTTGGAC 
GAGACTCTGC AGAACCCTGA GACCCAACAA AAACTAAAAG AGCAAACTAT TATGTTTGGC 
CAGCATATCA AAGGCTTTCC TGAAATAAAG TCAATTGATC CTTATAAGGC AGTGATTGAA 
GTTATTAAGA TGAGCAGAGA GGCAGTAGCT GAATCACTTT CCAGGGAAGA CAATGTTCAG 
TTCCAGCAAA ACTGCAATGG ATTCTTCATT GACATTCTAT GTACTCGGTT CTTTGAAGGC 
AACTCTCCCC CAGACAAAGA AGTTGTTGAT CAGCTGCTTT CTTCTCTTTT CATAACTGGT 
TCAGAGAATG ATGGAAACGA TCTGTCACCT TTTACTGCTG GAACTAATGA AAACCCTGCT 
ATCCGATCTG TGATCCTGAA GATGCTCCTC AAGTACAGCT TTGAAGAAAC ACAGAAATAT 
ATCCAGGATC ACTTAATCAG AATACAAGGA AGCAACTTGA CCACAGAAAT ACACATGCTC 
TTTATCAACT GCCTTGAGGA CTGTATGTAT GAAAGGTTTC AAGCTCTGCG TGAGGAAGAC 
AAATTGCATT ACCTCAAAGA CCAAGGTGAA TTTCTGAGAA ACTTCCTCCA AAATGGTCAT 
CCCCCTAATT CCCAAGAGAC CATGGTTGGG TTCCTGCAAG ATCTTGCCAA GGTTAGGTTT 
GCACTCAATG CAGTAGCGGA ACTGCTCTCT GAACCAGACA ATGTTGTAAA TGAGAAGAGG 
GAATTTCTCC AAAGTGGACA ACATTTTTGT GAACATTCAG CTAATGACTG GTACCGAGTG 
TATTTGATCC GCAAGATGGG AAATGAATCC GGAAGTGAAT ATGTCCAAAA TCTTAGTAAA 
GATGAAGAGT TTAGATGGTT ATTCCCACAT CAAATCTTTG GGGAAAGGGA GAACAACGCT 
AACCAGCTTG ACCGATTTTT GGTGCATAAG GATGGTTATA GGGAGTTAAG AGAAGGTGTT 
GGAGAAGTCA TAGTTTCAAG AAAGGAGGAG AGTCTTCAAC AAAGACTAGA GTTGGATGAA 
AGTTCTGCCA AGGAACAAGC TGTTTACCTG CTCCTCGCCA TTTTCTCTGA AGTAACAATG 
AGATACAGAG AAGCAAACAG GGTCGTCACT CAAGAAGAGA CTGATAATTT AAGGAATTTC 
ATTCAAAATA CCAATGTTCT CCAGGAGCAG TCAATTCAGC AATTCTCCCA GTCACTCCTA 
ACAAACTGCC GACCCCTAGT GAGAGTTGCT CCAGACATGA CAAGTTCTCA TTACACTATA 
GTGTCCCTGG CCGTACATCT TGGTGCTGTG CTTCTGAGCA CTGATAATGA TCTGTTTCAA 
CCACTGAGGA ATTTAGCCTT TTCCCCTGAC AGAATGCAGC AATCATTCCT ACCAACAATG 
CCACAGGACA TGCTAAACAA AGCTAGAGAA GTATTGGGAA ATGAAACTTG GTATGTCTGT 
GACAACAATC ATTATGTTCC AGTTCCTGGC TGTGGGATGC CAATGAGCTA CGCCAAGTGC 
CTAGAATGTG GAATTGAACT GGGGGGAGTT GACCACAAAA CTTATAAAGG AATGAGAAAG 
CTTGAACAGG ACATTGATGA GACAGGACAT ATTTTGGGAG CACCTGAGAC TCCAGTGTCA 
GTTGCGGCAG AAGACAGAGG CATGAGTGCT CCTGTATTTA ATGTATTGAG GCTGGTGACC 
CACCTGGCGA TGTTACTAGG GGCAGAACAA AATCGAGAGA ATGTGAAGAC CATGGTGAAA 
CCAGGAGTGG AAGATATTGG GACTTTCCTT TTCCGACATC TAGAGAAAAA TTTGGAACAA 
GTAAAGAACT CCCTAGGGGA AAGTGTGGAT AATACGGCCA CTGTACTCCA TCTTGTTTTC 
TGCAGGATGC TCACTATTTC CAATGATCAA CCTGACCAGC CTCTTTTACC TTTCAATCAT 
AATCTGTCAA CAAAGGACGT GAGAAGCAAC TGGGAGAGAA ATATGGCGGC ATCAGTCATC 
GCTCCGGTGC TTACGAATTT AAACCAAGAT CTTGATGATG TCAATAACTA CATAAGGAAT 
GACAAGAGAA TAAGTTCTGA CCCAATAATC AGAATCATAT ATGGGGAACC GTTGCATAAC 
ACTGATATTG TGGCATTGCC AGAAGATGGA CTCATTCCCT ACTCAAAAAT ATGGAGCTGC 
AGGACAAGAA TTACTGTTGG GCATCTTCAG CATATGGTTG AGCAGAAGGA TGGGGTGAAT 
AATCTGCCGA TGCTGTCAAC ATTCCTGAAG GATGAATGTG AACTCCGCCT GGTTAAGTTC 
CTACCAGAAA TATTAAATCT TCAGAAAGAG CTGGTAAAAA GATTCCAGAA CTTCCGAGAT 
GTGAACAAAC AAACAATTGG GGAATTTTTA ACCAGCATAC CTGTTGATGG TGCTCGACGT 
GTATTTGAGA AGAGAATACA AACTTTCCTA TTTACATGGA ATTGTCTAAG ACATTCTCTT 
CTTATGAAAG GGGAAATAAA ACTCCCAGAA GAAGCCTGTG CCAGTGACCT TACCCTAGCG 
AGTGACTTTG CCATTCTCCT TCCGAGTCGA CAAGGACAGG GTCTCTGTGC CACAGCATTG 
GTCAGCTATC TCATCGCTCT GCACAACAGC CTGGTTTATG CTGTGAGACG CTTCACCAGA 
GATGAGCAGA CATACTCTAT TGGGGCAGCT GAAGCCATGG ATCTACATGT GATCAGTTAT 
GAGGTGGAAA CAGATCTGAT GCCAATAATT CTGTCCAACT GTCAATACAA CACCCAAAGA 
GATGGGAAAG CCTTAGAGGA GATTGATCTG CAAAGGATCC AGCGTCAGAT CATCAGCCGA 
TTTCTCCAAG GCAAACCTCG CATTACCCTT GTAGGGCTGC CTACCATTGT CCACTCCCAA 
GATCGGAACT ACGAGAACAT ATTTAGAGAC GTGAGGGAAA AAGTCTTACA GGATCCACTG 
CCAAATTCAG CAATAGAAAA CATAAGCAAA GATCTGGATT TGTACAGCGA TGTCTGTGAT 
GCGCTTCAAA TCATTGAGAC TATGTTGGGG TTCTTAGCAA CATCCGGGGG CAATCCAGAT 
CTTCCGCTGA CCACTTATGT AGAAGATGTC CTACAGATGA AGGATCAGAG CGATGAGAAC 
ATCCTGGAGG CATTGAAGCA GTGTCACTTG AAGCACACAA TCTCACTATG GCAGCTTCTC 
ACCACTTTAC AGTCTGAACA CCGCATACGC CTTAAAAATG ATCCATTTGC AGATCTGGAT 
GAAGGGTATA AGCAGGAATT AGATGAGGAG AGTCAGAAGC TACTGACTGC GTTCTTGGCA 
CAACATGGAC TCAACGTCTT TCTTCTGGAA TTACATGAAA TGATCATCTT AAAGCTCAAG 
AAGCAACATG GGGCCAATGA TTTCCAACCT GCATGGGGGT TAAGAGAAGT TCTGGGCCCA 
TTGCTTGATG GGAAAGAGCA ACCATTCCCG GCGCTGGAGG AGGATTTCCC AGAGCAGATC 
TGTTTGGCTC ACTGTGTGGA GAGCTGGAAA GTGGCGGCCG CCATGAAGTG GAAACGACAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018087018.1
CDS	32..13174
Translation	MGKLYQHLINKNLSAVDAIIKDRLMAGLLCAKLIETYSLPADKDSLVKVCRLLSLEQMLNKNLPIQLEELKRIFSVYEGDVQKYMRSKESLQQAGRTLHRSWLCLLPLENLFTFLDTIPTDFRDAFTAAWYKFHTQSSSSKDKHIEKILTKLLNMAKGPSQGSLQKDSIYLCNISLKLHHLICTQTGLNKNSQLPLLSFKIALKLFGAAQKQEPMKGRETSINEVQSNLNKAVEMLKTWLQDFFGHRFNFRRYGYPTFGDELEFWNNLLMNDDNMDLKWKELLLSDLARKIKEESPVEQIKIFCREHSLLQRLHISIEECFEECAINAVHNAYKSHNNILNQISINNLQHFRKLVSTIIRNSWPKDVQGNPTENNNEILKHMLHWKDFTHIFKLYDPNQPITSQFIEETQKLVTLSDSVLLDTVCRLMHGDILYEHLKLVLGNKNQFLDICRLRETIKVEDVKKVLEWRNEELKTVNCKKTWVENLLKTCQNISKSVRVNTEEIQQILSVNLGAQKLNDLLPVKTLGATEPPEDKVTLFNSFTNSIMSMAESLHTHRHSHVFRMLWNEAVSSLSKRDENLENEEDDELTLDEMQESLFTPCFDKYTKIYEDIRSEEVTFEVLDTYFKTFKNHYQALHKELQTIRQLDPEDNGSWVSGRIRKIEQYHQLREAFESAQIVDDIRDFCKLLGDFKTLNTLQQFGADFSDYKEKPLSFITEDIVKTKEALCEITQERAEALKEALMRKEFIKWVRNSLEDVNELKVFVDLASISAGENDMDVDRVAQFHDAVLGYAPILYELKEEFGFNELMLCLKKLWKALESDPNLQKKLRDSARHIEWLKTVKESHGSVELSSLSLASVINQRGIYIIQEPNENLKINPDSVLRLEIPDDDLEQNQVRTFNLEELKELLSKLMLMSGKGDQGNAEVEKFSEIFSNVQRLAKAYIDLCLSGDILFKTWEATIYCSDEIDIAIRMKFKVTVTAIGEIGATGNIFELLPKICQTMEDILEKRQTFMDEKRSQHYFLNYYTAEQLVFLSQQIQKPDVSEELLVMLSFIKPECTKSDTQQALLAARRKGTSKLLATTNNSALFNFKKSTNVQEKLQIAWKYSMDCMNNLFPGCLDINTLGRSLTELAKKENSPVTRSLHPSLKPGCPNLVLCASSEILSSALAVYMQNLNQPLPSYDEVLLCSPKTTYEEVALFLRRCFTPGYSGKKIYSLLYAEDLSYDTAYRAEQLFYQLQKTKGLDYHLVIICDRERHHLHIPSTFSQYKVQVVPQRLFVEVQKYLLQHFTIDPNVVSAASVFKGVSVGIVTSKRAGVGKSLYVKRLYERLKLEFPNEKPLQKIIRMFQPQVDENKVLQKLLPFLTKENKNVPIIFHIDITSSVNRGISEFLFKLLILQYLKDDEGRMWKRQSCQLYLIEILESSCIISEKQKKPAPQMAQNIFMDFFPKISCCSPNEVLTKVTENQTGQNEDPGMDRKEFESECFQRPFQYLIRFEGKQSLDKFTYKEGSVEGNEAKCLQLFLLHCGVMDPSWAELRNFAWFLNVQLKDSENSVFCKPEFVEDAMQGFKNFVVDFMILMAKDFATPSLQITDESPGRQTCEEVCTNDGDLAPFQIRKKWEAEPHPYIFFNDDRMTITFIGFHLCVNSAGNVDAINPRDKSIIKKNIMTRQLYRDLMLQKVQFDIDFDTLPREEKINMLSRVLGINKPQDPDETYELTMDNTLKILAITMRFRCGIPVVIMGETGCGKTRLIKFICKLWKGNLNAENTTSDAENMKLVKVHGGTSAESINKNVLETQAIALTNKQNKCDTVLFFDEANTTQAISSIKEVLCDHTVDGLPLIENSGLQVIAACNPYRRHTEEMIRRLESAGLGYRVKADETNERLGSIPLRQLVYRVHPLPPSIMPLVWDFGQLNNETEKKYIQQIVQHLANEIEMHQSDTQILTNVLSASQSYMRNRNDECSFVSLRDVERCVEVFRWFYRNSDLLQANIENKKAWSLVLAIGVCYHASLEKKDAYRKAICQYFPEPYRNQRIILEKITSIQDLFLDRIQIRDNIARNLALKENLFMMVICIELRIPLFLVGKPGSSKSLSKTIVADAMQGQASHTELYKHLKQIHLVSFQCSPHSTPEGIIGTFRHCARFQKDKNLNEYASVVVLDEIGLAEDSPKMPLKTLHPLLEDGCIDDETSPYKKVGFIGISNWALDPAKMNRGLFVSRGDPDQNELIESAKGICSSDLLTIKEIDHHFKSFSDAYLKLCEKQDKEFFGLRDFYSLIKMVFTYAKHLKNEPTQLEIATAILRNFSGKDDLDALSIFITNQEANTYREEIKIMDLVMENIRNESADIEARYLLLLTRNYAALQILQQTLAKENQHPEIIFGSSFPKDQEYTQICRNINRVKICMETGQMVVLLNLQNLYESLYDALNQYYVYLAGQKYVDLGLGTHRVKCRVHPKFRLIVIEEKEIVYRDFPIPLINRLEKHYLDLNTVLERGQRAAVEELNDWVKDFTAGDREQLIGQKQDYKPADVLIGYHSDTCASVLLQITGKLKEECGDSDLYMAEVKEEAKATLLNCATPDAVIRLGRKELVDEYFNKQKHGSLVDFVKSHIHSGSKHQIAFTEITTFSRLLMSIDKRILEAETKDDVKSVEVLSLQQFDTEHSFLKKIRSFLKDSTGNKILIIQSDFEDGSQGAHLVASAKYATVNEINNLKKDNTEKTSTFVYFITKLPRIQGGSSYVGFHGGLWQSVHIDDLKEPSDKLPDVTALQNMTISQLFYGATAESQLRYVDGMEGGTDASYDTEPDQIPEVVNTTFLIRSCIQSAVGMITDEENTPSRSTRRIEILVNLLSTEDNLRASLLQTLKVRVYEILKNQEENSYNPNEWLVRAASNLDALQEAGTFRYTLWKRIQAAVIPIFAHLIYLMDCDANLEILVNPETEDFLKSLWIYIFKNKNLLNISSSLGNNSSQTIKATNYMNLLFCEGNSVPFSWRIKDYLEEITAQAQYIDSVEGPEMKFVNMFSNVPLGQYINNMEENDQQKLFQYYKRDFLLLTVNISSRDELKFLEISLSACIEEFRRSKEGEELTLPWVHLAYHHFQHRFQIFSRIVAISPTVLERMNEGFQEIEEILTNQMAIDIWAGFECLDILEATILDPNPQAWLNEVKNLQLTIELICSEKYLEGESDWCTQTVEQIRTMWHCIYAIALFTEHVILDETLQNPETQQKLKEQTIMFGQHIKGFPEIKSIDPYKAVIEVIKMSREAVAESLSREDNVQFQQNCNGFFIDILCTRFFEGNSPPDKEVVDQLLSSLFITGSENDGNDLSPFTAGTNENPAIRSVILKMLLKYSFEETQKYIQDHLIRIQGSNLTTEIHMLFINCLEDCMYERFQALREEDKLHYLKDQGEFLRNFLQNGHPPNSQETMVGFLQDLAKVRFALNAVAELLSEPDNVVNEKREFLQSGQHFCEHSANDWYRVYLIRKMGNESGSEYVQNLSKDEEFRWLFPHQIFGERENNANQLDRFLVHKDGYRELREGVGEVIVSRKEESLQQRLELDESSAKEQAVYLLLAIFSEVTMRYREANRVVTQEETDNLRNFIQNTNVLQEQSIQQFSQSLLTNCRPLVRVAPDMTSSHYTIVSLAVHLGAVLLSTDNDLFQPLRNLAFSPDRMQQSFLPTMPQDMLNKAREVLGNETWYVCDNNHYVPVPGCGMPMSYAKCLECGIELGGVDHKTYKGMRKLEQDIDETGHILGAPETPVSVAAEDRGMSAPVFNVLRLVTHLAMLLGAEQNRENVKTMVKPGVEDIGTFLFRHLEKNLEQVKNSLGESVDNTATVLHLVFCRMLTISNDQPDQPLLPFNHNLSTKDVRSNWERNMAASVIAPVLTNLNQDLDDVNNYIRNDKRISSDPIIRIIYGEPLHNTDIVALPEDGLIPYSKIWSCRTRITVGHLQHMVEQKDGVNNLPMLSTFLKDECELRLVKFLPEILNLQKELVKRFQNFRDVNKQTIGEFLTSIPVDGARRVFEKRIQTFLFTWNCLRHSLLMKGEIKLPEEACASDLTLASDFAILLPSRQGQGLCATALVSYLIALHNSLVYAVRRFTRDEQTYSIGAAEAMDLHVISYEVETDLMPIILSNCQYNTQRDGKALEEIDLQRIQRQIISRFLQGKPRITLVGLPTIVHSQDRNYENIFRDVREKVLQDPLPNSAIENISKDLDLYSDVCDALQIIETMLGFLATSGGNPDLPLTTYVEDVLQMKDQSDENILEALKQCHLKHTISLWQLLTTLQSEHRIRLKNDPFADLDEGYKQELDEESQKLLTAFLAQHGLNVFLLELHEMIILKLKKQHGANDFQPAWGLREVLGPLLDGKEQPFPALEEDFPEQICLAHCVESWKVAAAMKWKRH

Target ORF information:

RefSeq Version	XM_018231529.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin-protein ligase RNF213-like (LOC108699444), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018231529.1

ORF Insert Sequence:

ATGGGAAAAC TCTACCAACA TCTTATCAAC AAGAATTTAT CAGCAGTGGA TGCCATTATA 
AAAGACAGGC TGATGGCTGG ATTACTCTGC GCCAAACTGA TAGAAACCTA CTCACTTCCT 
GCTGACAAGG ATTCTCTTGT AAAGGTTTGC AGACTCCTCT CTTTGGAGCA GATGCTTAAC 
AAAAACCTAC CCATCCAACT TGAGGAACTG AAAAGAATCT TCTCAGTGTA CGAGGGAGAT 
GTCCAAAAAT ACATGAGGAG TAAGGAGAGT TTACAACAGG CAGGCAGAAC TTTACACAGA 
TCTTGGCTGT GTCTATTACC ACTGGAAAAC TTGTTCACGT TTTTGGATAC AATTCCCACC 
GATTTCCGAG ATGCTTTTAC CGCTGCCTGG TACAAATTTC ATACACAATC CTCAAGCAGC 
AAAGATAAGC ACATTGAGAA GATTCTAACA AAACTTCTGA ATATGGCAAA AGGACCGAGC 
CAGGGGTCAC TGCAGAAGGA CTCCATATAC CTGTGTAATA TCAGTTTGAA ACTTCATCAT 
TTAATCTGCA CTCAAACAGG GCTGAATAAA AACAGTCAGC TCCCACTTTT GTCTTTCAAG 
ATCGCACTAA AGCTTTTTGG AGCAGCACAG AAACAGGAGC CAATGAAAGG GAGGGAGACC 
AGTATCAATG AAGTGCAAAG CAATTTAAAC AAAGCTGTTG AGATGCTGAA GACATGGCTA 
CAGGACTTCT TTGGACATAG ATTTAATTTT AGACGCTATG GATATCCAAC ATTTGGGGAT 
GAACTTGAGT TTTGGAATAA CCTTCTTATG AATGACGACA ATATGGACCT GAAGTGGAAA 
GAACTGCTTC TCTCAGATCT GGCCAGGAAA ATAAAAGAGG AAAGCCCTGT GGAACAGATT 
AAAATCTTTT GCAGAGAACA TTCACTGTTG CAACGGCTTC ATATCTCCAT AGAAGAATGC 
TTTGAGGAAT GCGCCATTAA TGCTGTTCAT AATGCATATA AGTCTCATAA CAATATTCTC 
AACCAAATCT CAATAAACAA TCTACAACAT TTTAGAAAAC TGGTATCAAC AATAATAAGA 
AACTCTTGGC CAAAGGATGT GCAGGGAAAT CCAACTGAGA ATAATAATGA GATTTTAAAG 
CACATGCTGC ACTGGAAGGA TTTCACTCAT ATATTCAAAC TATATGATCC TAACCAACCA 
ATCACCTCTC AGTTTATAGA AGAAACTCAA AAGCTTGTTA CTTTGTCAGA TTCGGTCCTT 
CTTGACACCG TTTGTCGCCT AATGCATGGA GATATTCTGT ATGAACACCT CAAGTTGGTA 
CTGGGCAATA AAAACCAGTT CTTGGATATA TGTAGACTGA GAGAAACCAT AAAGGTGGAA 
GATGTTAAGA AAGTTCTGGA GTGGAGGAAT GAAGAGCTTA AAACAGTGAA CTGTAAGAAA 
ACATGGGTGG AGAACTTGCT GAAAACGTGC CAGAACATCA GCAAGTCTGT CCGAGTGAAT 
ACTGAGGAAA TTCAACAAAT CCTTTCTGTA AATCTGGGAG CACAAAAGTT AAATGATTTA 
TTACCTGTTA AGACACTTGG TGCTACTGAA CCTCCTGAAG ACAAGGTGAC ATTGTTTAAC 
AGTTTCACTA ATTCAATAAT GTCAATGGCC GAGAGTCTTC ACACCCACCG GCACAGTCAT 
GTTTTCCGCA TGTTGTGGAA TGAGGCTGTT AGCTCTCTTT CAAAGAGAGA TGAAAATTTA 
GAAAATGAAG AAGATGATGA ACTTACTTTG GATGAGATGC AGGAATCCTT GTTCACACCA 
TGTTTTGATA AATACACCAA GATATACGAG GACATCAGAT CTGAAGAAGT GACCTTTGAA 
GTCCTGGACA CATATTTCAA GACTTTCAAA AACCATTACC AAGCCCTACA TAAAGAACTA 
CAAACCATAC GCCAGTTAGA TCCAGAGGAT AATGGTTCCT GGGTCAGTGG CCGAATAAGG 
AAAATTGAGC AGTATCATCA GTTACGTGAA GCCTTTGAAT CAGCCCAGAT TGTTGATGAC 
ATCAGAGATT TCTGTAAACT TTTGGGAGAT TTTAAGACCC TGAATACTCT GCAACAATTT 
GGTGCTGACT TTAGTGATTA CAAGGAAAAA CCTTTATCTT TTATCACTGA AGACATTGTA 
AAGACAAAGG AAGCGCTTTG TGAGATAACA CAAGAACGTG CTGAGGCTCT GAAGGAGGCT 
TTAATGAGAA AGGAATTTAT AAAGTGGGTT CGAAACTCAC TGGAAGATGT CAATGAACTG 
AAAGTTTTTG TGGATCTGGC CTCCATTTCG GCTGGTGAGA ATGACATGGA TGTTGACCGT 
GTTGCTCAGT TCCATGATGC TGTTTTGGGC TACGCCCCTA TTTTGTATGA ACTCAAAGAG 
GAATTTGGAT TTAATGAATT AATGTTATGC TTGAAAAAAC TTTGGAAAGC CCTGGAAAGT 
GACCCAAATC TGCAGAAAAA ACTGAGAGAC TCTGCCCGTC ACATAGAGTG GCTGAAAACT 
GTAAAAGAAA GTCATGGATC TGTGGAACTC TCATCACTGT CTCTGGCATC TGTTATAAAC 
CAAAGAGGCA TCTACATTAT TCAAGAGCCC AATGAGAACC TAAAGATAAA CCCAGACAGT 
GTCCTGAGAC TAGAAATTCC TGATGATGAT CTGGAACAGA ACCAGGTGCG CACCTTTAAT 
TTAGAAGAAC TGAAGGAGCT GTTGAGCAAG CTCATGCTTA TGTCTGGGAA AGGTGACCAG 
GGTAATGCTG AAGTTGAAAA GTTTTCAGAG ATTTTCTCCA ATGTTCAGAG ACTTGCCAAG 
GCCTACATTG ATCTCTGCTT GTCTGGAGAC ATTTTATTCA AAACTTGGGA GGCCACTATC 
TACTGCTCAG ATGAGATTGA TATTGCTATT AGGATGAAAT TTAAAGTAAC AGTAACAGCC 
ATTGGGGAGA TAGGAGCAAC AGGAAATATT TTTGAACTGC TTCCCAAAAT CTGCCAAACC 
ATGGAAGACA TATTAGAAAA AAGGCAAACT TTTATGGATG AAAAACGTTC TCAGCATTAC 
TTTCTAAATT ACTACACCGC AGAGCAGTTG GTCTTCCTTA GCCAGCAGAT TCAGAAGCCT 
GATGTTAGTG AAGAGCTGCT TGTTATGTTA TCCTTTATCA AGCCTGAATG TACAAAATCT 
GACACCCAGC AAGCTTTATT AGCTGCACGG AGGAAAGGAA CATCAAAGCT CCTTGCGACT 
ACTAATAATT CTGCTTTGTT TAACTTTAAA AAATCTACCA ATGTTCAAGA GAAACTACAG 
ATTGCTTGGA AATACTCCAT GGACTGCATG AACAATTTGT TTCCTGGATG TCTGGATATA 
AATACACTTG GAAGGTCCTT AACTGAACTG GCAAAAAAGG AAAATAGCCC AGTGACCAGA 
AGTCTACATC CCAGTCTAAA ACCGGGATGT CCCAATTTGG TACTCTGTGC ATCATCAGAG 
ATTCTATCTT CAGCTCTTGC TGTATATATG CAGAATTTAA ATCAGCCACT TCCATCTTAT 
GATGAAGTTC TTCTCTGTTC ACCTAAAACT ACTTACGAGG AGGTGGCATT GTTTTTGAGA 
AGATGTTTCA CACCAGGCTA CAGTGGGAAG AAGATTTACA GTTTGCTGTA TGCAGAAGAT 
CTCAGCTATG ATACAGCCTA TAGAGCTGAA CAACTGTTTT ACCAACTGCA AAAGACAAAA 
GGCCTAGACT ACCACCTTGT GATTATCTGT GACCGTGAGA GACATCATTT GCATATACCA 
TCTACCTTTA GCCAATACAA AGTGCAAGTT GTTCCTCAGA GACTTTTTGT TGAAGTTCAA 
AAATATTTAT TGCAACATTT TACTATTGAT CCAAATGTAG TCTCAGCTGC CTCCGTTTTT 
AAAGGCGTGA GTGTGGGAAT AGTGACCTCC AAACGAGCAG GAGTTGGAAA GTCGCTCTAT 
GTGAAGAGAC TATATGAAAG GCTGAAACTG GAATTTCCAA ATGAAAAGCC TCTCCAAAAA 
ATAATCCGAA TGTTTCAGCC CCAGGTGGAC GAAAACAAAG TTCTGCAAAA GCTTCTTCCA 
TTTCTAACCA AGGAAAATAA AAATGTGCCC ATAATATTTC ATATTGATAT CACCTCTTCT 
GTTAACAGGG GAATTTCAGA ATTTCTTTTC AAACTTTTGA TTCTTCAATA TCTAAAAGAT 
GATGAAGGGA GAATGTGGAA GCGCCAGTCT TGTCAGCTTT ACCTTATTGA AATCTTAGAA 
TCTTCTTGCA TAATTTCTGA GAAGCAGAAA AAACCTGCAC CTCAGATGGC GCAAAATATT 
TTTATGGACT TTTTCCCTAA AATATCATGC TGTTCTCCAA ATGAGGTGCT GACAAAAGTC 
ACAGAAAATC AGACTGGACA AAATGAAGAT CCTGGAATGG ATAGAAAGGA ATTTGAGAGT 
GAATGCTTTC AGCGGCCTTT TCAGTACTTG ATACGTTTTG AAGGGAAACA AAGTCTGGAT 
AAATTTACAT ACAAGGAAGG TTCCGTAGAA GGAAATGAGG CAAAATGTCT GCAGTTGTTC 
CTTTTGCACT GTGGGGTTAT GGACCCATCA TGGGCAGAAC TTCGAAATTT TGCATGGTTC 
TTAAATGTTC AGTTAAAAGA CAGTGAGAAT TCTGTTTTCT GCAAACCAGA ATTTGTGGAA 
GATGCTATGC AGGGCTTCAA GAATTTTGTT GTAGACTTTA TGATATTGAT GGCAAAAGAT 
TTTGCAACTC CTTCATTACA GATCACTGAT GAGAGTCCAG GCAGACAAAC TTGTGAAGAA 
GTCTGTACTA ATGATGGAGA CCTTGCTCCA TTTCAGATAC GTAAAAAATG GGAGGCTGAG 
CCACATCCCT ACATTTTCTT CAATGATGAT CGCATGACAA TAACATTTAT CGGCTTTCAC 
TTGTGCGTGA ACAGTGCTGG AAATGTTGAT GCGATCAATC CTAGAGATAA GAGCATTATT 
AAGAAAAATA TTATGACAAG ACAACTTTAT CGAGACTTAA TGCTTCAGAA AGTTCAGTTC 
GATATAGACT TTGACACTCT ACCTAGGGAG GAAAAGATTA ATATGTTATC CAGAGTTTTG 
GGGATTAATA AACCGCAGGA TCCAGATGAA ACATATGAGT TGACTATGGA TAACACTCTG 
AAGATCCTGG CTATTACAAT GAGGTTCCGG TGTGGAATTC CTGTTGTCAT TATGGGAGAA 
ACAGGTTGTG GAAAAACAAG ACTCATAAAG TTTATTTGTA AATTATGGAA AGGAAACCTG 
AATGCAGAAA ATACTACATC TGATGCAGAA AATATGAAAC TTGTAAAGGT TCATGGCGGT 
ACAAGTGCTG AGAGTATTAA CAAAAATGTG CTGGAGACAC AAGCCATTGC CCTCACTAAC 
AAACAGAACA AATGTGATAC AGTGTTGTTT TTTGATGAAG CAAACACAAC TCAAGCCATA 
AGCAGTATCA AAGAAGTCCT GTGTGACCAC ACTGTGGATG GCTTGCCTTT AATTGAAAAC 
TCAGGTTTGC AAGTCATTGC TGCATGTAAC CCCTACAGGA GGCATACAGA AGAAATGATC 
AGACGATTGG AATCTGCTGG TTTAGGGTAC AGAGTAAAAG CAGATGAAAC TAATGAACGA 
CTTGGGTCCA TCCCATTAAG ACAATTAGTC TATCGTGTTC ATCCATTGCC TCCAAGTATA 
ATGCCACTGG TTTGGGATTT TGGTCAACTA AATAATGAAA CCGAAAAGAA ATACATTCAA 
CAGATAGTAC AGCATTTGGC AAATGAGATT GAAATGCATC AGAGCGATAC ACAGATATTG 
ACCAATGTGC TTTCAGCTTC CCAGTCATAT ATGAGAAATA GAAATGACGA ATGCAGCTTT 
GTAAGCCTTC GAGATGTTGA ACGTTGTGTA GAAGTGTTCA GGTGGTTCTA CCGCAATAGT 
GACCTCTTGC AGGCAAATAT AGAAAATAAA AAGGCGTGGT CTCTTGTTTT GGCCATTGGG 
GTATGTTACC ATGCCTCATT AGAAAAGAAA GATGCATACA GAAAGGCAAT TTGCCAATAT 
TTTCCTGAAC CCTACAGAAA CCAAAGAATA ATTCTTGAGA AAATAACAAG TATCCAAGAC 
CTCTTTCTAG ATCGTATACA AATAAGAGAC AACATTGCCA GGAATCTTGC TTTGAAGGAA 
AATCTATTCA TGATGGTGAT TTGCATTGAG CTCAGAATTC CATTATTTCT TGTTGGAAAG 
CCTGGAAGCT CAAAATCCCT TTCAAAGACC ATTGTTGCCG ATGCAATGCA AGGCCAAGCA 
TCACACACAG AACTCTACAA ACATCTTAAG CAAATACACC TTGTATCTTT TCAGTGTAGT 
CCTCACTCAA CTCCAGAAGG CATCATTGGC ACTTTCAGGC ACTGTGCTCG CTTCCAGAAA 
GACAAGAACT TAAATGAGTA TGCTTCTGTG GTAGTGCTAG ATGAAATAGG GCTGGCAGAA 
GACTCTCCTA AAATGCCTTT AAAAACATTG CACCCTTTGC TGGAGGATGG TTGTATAGAT 
GATGAGACAT CACCTTACAA AAAAGTTGGC TTCATAGGAA TTTCTAACTG GGCGCTAGAT 
CCTGCAAAAA TGAATAGAGG CTTATTTGTA TCCCGTGGAG ACCCTGATCA AAATGAGCTT 
ATTGAAAGTG CAAAGGGAAT CTGCTCGTCT GATCTCTTGA CCATTAAGGA AATTGACCAT 
CATTTCAAAA GTTTTTCCGA TGCTTATCTG AAACTGTGTG AAAAACAAGA TAAAGAATTC 
TTTGGTTTAC GAGATTTTTA CAGTTTGATA AAAATGGTGT TTACCTATGC GAAACACTTA 
AAAAATGAGC CAACACAGCT TGAGATTGCA ACAGCAATAC TGAGGAATTT CAGTGGGAAG 
GATGACTTGG ATGCCTTGAG TATTTTTATA ACAAATCAAG AAGCAAACAC ATATAGGGAG 
GAAATTAAAA TAATGGATTT GGTAATGGAG AACATAAGAA ATGAGAGTGC TGATATAGAG 
GCTCGATACC TGTTGTTATT AACTAGAAAT TACGCAGCAC TGCAGATTCT ACAACAGACA 
CTGGCTAAAG AAAATCAACA TCCTGAGATT ATATTTGGGT CCAGCTTTCC AAAAGACCAA 
GAATATACTC AGATCTGCAG AAACATCAAC CGTGTTAAAA TCTGTATGGA AACAGGACAG 
ATGGTTGTCC TTCTTAATCT CCAAAATCTT TATGAGAGCC TCTATGATGC ATTAAATCAA 
TATTATGTCT ATCTAGCTGG ACAAAAATAT GTTGACTTGG GTTTAGGCAC ACATCGGGTA 
AAATGTAGAG TCCACCCAAA GTTTAGACTG ATAGTCATTG AAGAGAAAGA AATTGTGTAC 
AGAGATTTTC CAATTCCTCT TATTAACAGA CTTGAGAAAC ATTATCTGGA TCTAAACACA 
GTTCTAGAGA GGGGACAAAG AGCCGCTGTA GAGGAATTGA ATGACTGGGT TAAAGATTTC 
ACTGCAGGCG ACAGAGAACA ACTAATTGGC CAGAAACAGG ACTATAAACC TGCAGATGTC 
CTTATTGGTT ACCATTCTGA CACCTGTGCA TCAGTGTTGT TACAAATAAC AGGAAAACTG 
AAAGAGGAAT GTGGTGACAG TGACCTTTAC ATGGCAGAAG TGAAAGAGGA GGCAAAGGCT 
ACTCTGCTGA ACTGTGCAAC ACCCGATGCT GTCATTCGAC TGGGCAGAAA AGAGTTGGTC 
GATGAGTACT TCAACAAACA GAAACATGGA TCTCTGGTTG ACTTTGTTAA GAGCCACATC 
CATTCAGGAT CTAAACATCA AATTGCATTT ACAGAGATCA CTACATTTTC ACGCCTTTTG 
ATGTCTATTG ACAAAAGAAT TTTGGAAGCC GAGACAAAGG ATGATGTCAA AAGTGTAGAG 
GTGCTGTCAT TGCAACAGTT TGATACGGAA CATTCTTTCC TGAAAAAAAT CAGGAGTTTT 
CTAAAAGATT CCACTGGTAA TAAAATACTG ATCATACAGT CAGACTTTGA AGATGGATCT 
CAAGGAGCCC ATTTGGTTGC ATCTGCCAAG TACGCCACTG TAAATGAAAT AAACAACTTA 
AAGAAAGACA ACACTGAGAA GACATCAACC TTTGTCTATT TTATAACTAA ACTTCCTAGA 
ATACAAGGGG GGTCATCATA TGTGGGATTC CATGGAGGAC TTTGGCAATC TGTTCATATT 
GATGACTTAA AGGAACCAAG CGATAAGTTG CCAGATGTAA CTGCCCTTCA AAACATGACA 
ATAAGCCAAT TATTTTATGG AGCCACAGCT GAATCACAGT TAAGATATGT TGATGGCATG 
GAAGGTGGCA CAGACGCTAG TTATGACACT GAGCCAGACC AGATACCAGA GGTAGTAAAC 
ACCACCTTTC TCATTCGGAG CTGCATACAG AGTGCAGTGG GCATGATTAC AGATGAAGAG 
AATACTCCCA GCCGCAGCAC AAGGAGGATT GAAATTCTAG TTAATCTACT GAGCACCGAG 
GACAATCTAA GAGCTTCCCT TCTACAAACA CTGAAAGTTC GTGTTTATGA AATTCTGAAG 
AACCAGGAAG AGAATTCATA TAACCCAAAT GAGTGGCTTG TAAGAGCTGC CTCCAACCTT 
GATGCATTGC AAGAAGCTGG CACATTCCGA TACACTCTTT GGAAACGTAT TCAGGCTGCT 
GTGATCCCAA TATTTGCACA TCTCATTTAT CTGATGGATT GTGATGCCAA CCTGGAGATT 
CTGGTGAACC CAGAAACTGA GGACTTTCTG AAATCTTTAT GGATTTACAT TTTCAAAAAT 
AAAAATCTTC TAAATATTTC CAGCAGCCTT GGTAATAATA GTTCTCAGAC CATAAAGGCA 
ACTAATTACA TGAACCTGTT GTTTTGTGAG GGGAATTCAG TTCCGTTCAG CTGGAGAATT 
AAAGATTATT TGGAAGAAAT TACAGCACAA GCTCAGTACA TTGATAGTGT AGAAGGTCCA 
GAGATGAAGT TTGTAAATAT GTTTAGCAAT GTGCCTTTGG GACAATATAT AAATAACATG 
GAGGAAAATG ATCAACAAAA GCTGTTCCAG TACTACAAAC GGGACTTTTT ACTTCTTACT 
GTGAATATAT CCTCCAGGGA TGAACTCAAG TTTTTAGAGA TTTCGCTTTC CGCTTGTATT 
GAAGAATTTA GAAGAAGTAA AGAAGGAGAA GAGCTGACAC TTCCCTGGGT GCATCTCGCT 
TATCACCATT TTCAGCACAG GTTCCAAATA TTCTCTAGGA TTGTAGCGAT CAGTCCAACT 
GTTCTGGAGA GGATGAATGA AGGTTTCCAA GAGATAGAAG AGATTCTAAC AAATCAGATG 
GCTATTGATA TATGGGCTGG ATTTGAATGC TTAGACATTT TGGAAGCCAC CATTCTGGAC 
CCAAACCCAC AAGCATGGTT GAATGAAGTA AAAAACCTTC AGCTGACAAT TGAACTCATT 
TGCTCAGAAA AATACTTAGA AGGGGAAAGT GACTGGTGCA CACAAACAGT TGAGCAGATC 
CGAACCATGT GGCATTGTAT CTACGCTATT GCATTATTTA CAGAACATGT CATTTTGGAC 
GAGACTCTGC AGAACCCTGA GACCCAACAA AAACTAAAAG AGCAAACTAT TATGTTTGGC 
CAGCATATCA AAGGCTTTCC TGAAATAAAG TCAATTGATC CTTATAAGGC AGTGATTGAA 
GTTATTAAGA TGAGCAGAGA GGCAGTAGCT GAATCACTTT CCAGGGAAGA CAATGTTCAG 
TTCCAGCAAA ACTGCAATGG ATTCTTCATT GACATTCTAT GTACTCGGTT CTTTGAAGGC 
AACTCTCCCC CAGACAAAGA AGTTGTTGAT CAGCTGCTTT CTTCTCTTTT CATAACTGGT 
TCAGAGAATG ATGGAAACGA TCTGTCACCT TTTACTGCTG GAACTAATGA AAACCCTGCT 
ATCCGATCTG TGATCCTGAA GATGCTCCTC AAGTACAGCT TTGAAGAAAC ACAGAAATAT 
ATCCAGGATC ACTTAATCAG AATACAAGGA AGCAACTTGA CCACAGAAAT ACACATGCTC 
TTTATCAACT GCCTTGAGGA CTGTATGTAT GAAAGGTTTC AAGCTCTGCG TGAGGAAGAC 
AAATTGCATT ACCTCAAAGA CCAAGGTGAA TTTCTGAGAA ACTTCCTCCA AAATGGTCAT 
CCCCCTAATT CCCAAGAGAC CATGGTTGGG TTCCTGCAAG ATCTTGCCAA GGTTAGGTTT 
GCACTCAATG CAGTAGCGGA ACTGCTCTCT GAACCAGACA ATGTTGTAAA TGAGAAGAGG 
GAATTTCTCC AAAGTGGACA ACATTTTTGT GAACATTCAG CTAATGACTG GTACCGAGTG 
TATTTGATCC GCAAGATGGG AAATGAATCC GGAAGTGAAT ATGTCCAAAA TCTTAGTAAA 
GATGAAGAGT TTAGATGGTT ATTCCCACAT CAAATCTTTG GGGAAAGGGA GAACAACGCT 
AACCAGCTTG ACCGATTTTT GGTGCATAAG GATGGTTATA GGGAGTTAAG AGAAGGTGTT 
GGAGAAGTCA TAGTTTCAAG AAAGGAGGAG AGTCTTCAAC AAAGACTAGA GTTGGATGAA 
AGTTCTGCCA AGGAACAAGC TGTTTACCTG CTCCTCGCCA TTTTCTCTGA AGTAACAATG 
AGATACAGAG AAGCAAACAG GGTCGTCACT CAAGAAGAGA CTGATAATTT AAGGAATTTC 
ATTCAAAATA CCAATGTTCT CCAGGAGCAG TCAATTCAGC AATTCTCCCA GTCACTCCTA 
ACAAACTGCC GACCCCTAGT GAGAGTTGCT CCAGACATGA CAAGTTCTCA TTACACTATA 
GTGTCCCTGG CCGTACATCT TGGTGCTGTG CTTCTGAGCA CTGATAATGA TCTGTTTCAA 
CCACTGAGGA ATTTAGCCTT TTCCCCTGAC AGAATGCAGC AATCATTCCT ACCAACAATG 
CCACAGGACA TGCTAAACAA AGCTAGAGAA GTATTGGGAA ATGAAACTTG GTATGTCTGT 
GACAACAATC ATTATGTTCC AGTTCCTGGC TGTGGGATGC CAATGAGCTA CGCCAAGTGC 
CTAGAATGTG GAATTGAACT GGGGGGAGTT GACCACAAAA CTTATAAAGG AATGAGAAAG 
CTTGAACAGG ACATTGATGA GACAGGACAT ATTTTGGGAG CACCTGAGAC TCCAGTGTCA 
GTTGCGGCAG AAGACAGAGG CATGAGTGCT CCTGTATTTA ATGTATTGAG GCTGGTGACC 
CACCTGGCGA TGTTACTAGG GGCAGAACAA AATCGAGAGA ATGTGAAGAC CATGGTGAAA 
CCAGGAGTGG AAGATATTGG GACTTTCCTT TTCCGACATC TAGAGAAAAA TTTGGAACAA 
GTAAAGAACT CCCTAGGGGA AAGTGTGGAT AATACGGCCA CTGTACTCCA TCTTGTTTTC 
TGCAGGATGC TCACTATTTC CAATGATCAA CCTGACCAGC CTCTTTTACC TTTCAATCAT 
AATCTGTCAA CAAAGGACGT GAGAAGCAAC TGGGAGAGAA ATATGGCGGC ATCAGTCATC 
GCTCCGGTGC TTACGAATTT AAACCAAGAT CTTGATGATG TCAATAACTA CATAAGGAAT 
GACAAGAGAA TAAGTTCTGA CCCAATAATC AGAATCATAT ATGGGGAACC GTTGCATAAC 
ACTGATATTG TGGCATTGCC AGAAGATGGA CTCATTCCCT ACTCAAAAAT ATGGAGCTGC 
AGGACAAGAA TTACTGTTGG GCATCTTCAG CATATGGTTG AGCAGAAGGA TGGGGTGAAT 
AATCTGCCGA TGCTGTCAAC ATTCCTGAAG GATGAATGTG AACTCCGCCT GGTTAAGTTC 
CTACCAGAAA TATTAAATCT TCAGAAAGAG CTGGTAAAAA GATTCCAGAA CTTCCGAGAT 
GTGAACAAAC AAACAATTGG GGAATTTTTA ACCAGCATAC CTGTTGATGG TGCTCGACGT 
GTATTTGAGA AGAGAATACA AACTTTCCTA TTTACATGGA ATTGTCTAAG ACATTCTCTT 
CTTATGAAAG GGGAAATAAA ACTCCCAGAA GAAGCCTGTG CCAGTGACCT TACCCTAGCG 
AGTGACTTTG CCATTCTCCT TCCGAGTCGA CAAGGACAGG GTCTCTGTGC CACAGCATTG 
GTCAGCTATC TCATCGCTCT GCACAACAGC CTGGTTTATG CTGTGAGACG CTTCACCAGA 
GATGAGCAGA CATACTCTAT TGGGGCAGCT GAAGCCATGG ATCTACATGT GATCAGTTAT 
GAGGTGGAAA CAGATCTGAT GCCAATAATT CTGTCCAACT GTCAATACAA CACCCAAAGA 
GATGGGAAAG CCTTAGAGGA GATTGATCTG CAAAGGATCC AGCGTCAGAT CATCAGCCGA 
TTTCTCCAAG GCAAACCTCG CATTACCCTT GTAGGGCTGC CTACCATTGT CCACTCCCAA 
GATCGGAACT ACGAGAACAT ATTTAGAGAC GTGAGGGAAA AAGTCTTACA GGATCCACTG 
CCAAATTCAG CAATAGAAAA CATAAGCAAA GATCTGGATT TGTACAGCGA TGTCTGTGAT 
GCGCTTCAAA TCATTGAGAC TATGTTGGGG TTCTTAGCAA CATCCGGGGG CAATCCAGAT 
CTTCCGCTGA CCACTTATGT AGAAGATGTC CTACAGATGA AGGATCAGAG CGATGAGAAC 
ATCCTGGAGG CATTGAAGCA GTGTCACTTG AAGCACACAA TCTCACTATG GCAGCTTCTC 
ACCACTTTAC AGTCTGAACA CCGCATACGC CTTAAAAATG ATCCATTTGC AGATCTGGAT 
GAAGGGTATA AGCAGGAATT AGATGAGGAG AGTCAGAAGC TACTGACTGC GTTCTTGGCA 
CAACATGGAC TCAACGTCTT TCTTCTGGAA TTACATGAAA TGATCATCTT AAAGCTCAAG 
AAGCAACATG GGGCCAATGA TTTCCAACCT GCATGGGGGT TAAGAGAAGT TCTGGGCCCA 
TTGCTTGATG GGAAAGAGCA ACCATTCCCG GCGCTGGAGG AGGATTTCCC AGAGCAGATC 
TGTTTGGCTC ACTGTGTGGA GAGCTGGAAA GTGGCGGCCG CCATGAAGTG GAAACGACAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC108699444 cDNA ORF clone, Xenopus laevis(African clawed frog)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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