LOC108695408 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108695408
Entrez Gene ID	108695408
Full Name	ubiquitin protein ligase E3 component n-recognin 5 S homeolog
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 5 S homeolog Names E3 ubiquitin-protein ligase UBR5-like
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018223898.1	XP_018079387.1	E3 ubiquitin-protein ligase UBR5-like isoform X4
XM_018223899.1	XP_018079388.1	E3 ubiquitin-protein ligase UBR5-like isoform X5
XM_018223900.1	XP_018079389.1	E3 ubiquitin-protein ligase UBR5-like isoform X6
XM_018223901.1	XP_018079390.1	E3 ubiquitin-protein ligase UBR5-like isoform X7
XM_018223896.1	XP_018079385.1	E3 ubiquitin-protein ligase UBR5-like isoform X2
XM_018223895.1	XP_018079384.1	E3 ubiquitin-protein ligase UBR5-like isoform X1
XM_018223902.1	XP_018079391.1	E3 ubiquitin-protein ligase UBR5-like isoform X8
XM_018223897.1	XP_018079386.1	E3 ubiquitin-protein ligase UBR5-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC108695408 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108695408 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAf37363	XM_018223895.1 Latest version!	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAf37364	XM_018223896.1 Latest version!	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAf37365	XM_018223897.1 Latest version!	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAf37366	XM_018223898.1 Latest version!	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAf37367	XM_018223899.1 Latest version!	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAf37368	XM_018223900.1 Latest version!	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAf37369	XM_018223901.1 Latest version!	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OAf37370	XM_018223902.1 Latest version!	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108695408 ( XM_018223895.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018079384.1 Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X1, mRNA.

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CloneID	OAf37363
Clone ID Related Accession (Same CDS sequence)	XM_018223895.1
Accession Version	XM_018223895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase UBR5-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAAGA TCTTATGTCA CTGCTGGATG CTGACATTCA TTCTGCTCAC 
CCAAGTGTCA TAATTGATGC TGATGCTATG TTTTCAGAAG ATATTAGCTA CTTTGGTTAT 
CCATCTTTTC GGCGCTCTTC ACTTTCCAGG ATAGGCTCAT CCAGAGTTCT CCTTCTTCCC 
TTAGAGAGAG ACTCTGAGCT GTTGCGTGAA CGTGAGTCAG TGTTGCGTTT GCGTGAGCGG 
AGGTGGCTTG ATGGGGCCTC GTTTGATAAT GAAAGAGGCT CCACAAGTAA AGAAGGGGAA 
CCTACACTCG ATAAGAAGAA CACTCCCATT ATGAGCCCAG TGTCACTGGG AGAAGACCTG 
CAATGGTGGC CTGAAAAGGA TGGGACAAAG TTTATTTCTA TTGGAGCACT TTATTCCGAG 
CTGGTTGCTG TAAGCAGTAA GGGAGAACTT TATCAGTGGA AGTGGATCGA GGTGGAGCCT 
TATAGGAATA CACAGAATCC ATCATTACGT CATCCAAGAA CATCCTTTTT AGGCCTAACA 
AATGAGAAAA TAGTCCTGTT GTCAGCAAAC AGTATCCGAG CCACAGTCGC TACAGAAAGC 
AACAAGGTTG CCACTTGGGT CGATGAAACA TTAAGCTCGG TAGCCTCAAA GTTAGAACAC 
ACAGCTCAGA TTTTCCCTGA GCTACAAGGA GACAAGATTG TCTCGTTGCA CTGTTGTGCA 
CTTTATACTT GTGCGCAATT AGAAAATAAC CTTTATTGGT GGGGTGTGGT TCCTTTCAGT 
CAGAGGAAGA AAATGCTTGA AAAAGCTCGT GCAAAGAATA AAAAACCCAA ATCTAGTGCC 
GGAATTTCAT CCATGCCAAA TATCACAGTT GGCACCCAGG TATGCTTAAG AAATAACCCT 
CTCTACCATG CGGGAGCTGT TGCTTTCTCG ATCAGTGCTG GCATTCCCAA AGTAGGAGTA 
TTAATGGAAT CTGTGTGGAA TATGAATGAT AGCTGCAGAT TTCAGCTCCG TTCTCCAGAG 
AGTTTAAAAA ACTTGGAAAA AACAAATAAA ACAATTGAAG CCAAACCTGA GAGCAAACAG 
GAACCTGTAA AAACAGAGAT GGGCCCGCCC CCATCTCCCG CATCTACCTG TAGTGATGCC 
TCTTCAATTG CTAGCAGTGC CTCCATGCCA TACAAACGAA GACGATCAAC TCCTGCTCCT 
AAGGAAGAAG AGAGAGTGAA TGAAGAACAG TGGTCTCTCA GAGAAGTTGT TTTTGTAGAA 
GATGTTAAAA ATGTTCCTGT TGGGAAGGTA CTAAAAGTTG ATGGGGCCTA TGTTGCAGTC 
AAATTTCCTG GCACTTCTAA TAATTCTAAT AATTCTAATA ATTCTAACAA TCCGTGCACC 
CCTGGCTCTG ATCCAGACCC ATCCTCTCTC CTCCAGGATT GTAGATTACT ACGGATTGAT 
GAACTACAGG TAGTGAAAAC TGGGGGGACA CCCAAAGTCC CAGACTGTTT CCAAAGGACA 
CCTAAAAAGC TGTGCATTCC TGAAAAAACT GAGATCCTGG CAGTCAATGT AGATTCCAAA 
GGGGTTCACG CGGTACTGAA AACAGGGACA TGGGTACGGT ACTGTATCTT TGATCTTGCA 
ACTGGAAAAG CAGAACAGGA AAACAACTTC CCAACAAGTA GCATTGCATT TCTTGGCCAG 
AATGAGAGAA ATGTTGCTAT TTTTACAGCT GGGCAGGAAT CTCCCATCAT TCTTAGAGAT 
GGCAATGGTA CCATTTATCC CATGGCAAAA GATTGCATGG GTGGGATAAG AGATCCAGAC 
TGGCTTGACC TTCCTCCAAT AAGCAGTCTT GGAATGGGAG TTCATTCTTT AACCAATCTC 
CCTGCAAATT CAACAATTAA GAAGAAAGCA GCCATTATCA TAATGGCTGT TGAGAAGCAA 
ACACTGATGC AACACATTTT GCGCGGTGAT TATGAGGCCT GCAGGCAGTA TTTGATGAAC 
CTAGAGCAAG GTGTAGTTTT GGATCCAAAC ACTCAAATGC TGCAGACTTT TCTGAGCCAC 
AGATGTGATG GCAATAGGAA CATATTGCAT GCCTGCGTGT CCGTTTGCTT CCCAACAAGC 
AACAAGGAAA CAAAAGAAGA GGAGGAAGCA GAGCGCTCTG AAAGGAATAC ATTTGCAGAA 
AGACTGTCTG CAGTAGAAGC AATTGCCAAT GCTATATCTG TGGTTTCTAG CAATGGTCCC 
GGAAATCGTG CTGGATCGTC CAGCAGTAGA AGCTTGAGAT TGAGGGAAAT GATGAGGAGG 
TCCCTTCGGG CAGCAGGTTT AGGAAGACAT GAGGCTGGTG CTTCTAGTGA TCACCAAGAC 
CCAGTATCTC CTCCCATTGC ACCTCCTAGC TGGGTTCCTG ATCCACCTGC TATGGATCCA 
GATGGTGACA TAGATTTTAT CCTGGCCCCT GCTGTAGGAT CTCTTACCAC TGCATCCACT 
GTACCTGGCC AAGGACCAAA TACTTCTACA ATACCAGGTC CTTCCTCAGA GCCATCAGTT 
GTTGAGTCCA AGGATCGGAA GGCAAATGCT CACTTCATAC TGAAGTTATT ATGCGATAGC 
ATCGTTCTGC AGCCTTACCT ACGTGATCTC CTGTCTGCCA AGGATGCAAG AGGTATGACT 
CCCTTCATGT CTGCTGTTAG TGGAAGAGCC TATCCAGCTG CAATTACCAT ACTGGAAACT 
GCACAGAAAA TCGCAAAAGC TGAAGCAAAT TCAAGTGAGA AAGAGGAGGA TATTTTCAAG 
GGAATGGTAT GTCCACCTGG TACCAATGCA GACGACTCTC CTCTATATGT ACTTTGCTGC 
AATGATACCT GCAGTTTTAC ATGGACAGGA GCTGAGCATA TCAATCAGGA CATATTTGAA 
TGTCGAACAT GTGGCTTGTT GGAATCCCTG TGCTGCTGTA CCGAGTGTGC AAGAGTCTGC 
CACAAAGGCC ATGACTGCAA ATTGAAAAGA ACATCACCTA CTGCTTACTG CGACTGTTGG 
GAGAAATGCA AATGCAAAAC ATTAATTGCC GGGCAAAAGT CTGCACGACT TGATCTGCTC 
TATCGACTGC TGACCATCAC TAATCTTGTT ACAATGCCAA ATAGCAGGGG AGAGCACCTA 
CTTCTTTTCT TAGTGCAGAC TGTAGCCAGA CAGACAGTGG AGCACTGCCA GTACCGACCT 
CCAAGGATTC GAGAAGATCG CAATCGAAAG GCAGCTAACC CTGAAGATTC CGACATGCCA 
GATCATGACC TAGAGCCCCC TAGGTTTGCC CAGCTTGCAC TGGAACGTGT TTTACAAGAT 
TGGAACGCAC TCAAATCCAT GATTATGTTT GGATCCCAAG AAAATAAAGA CCCCCTTAGT 
GCTAGCAGTA GAATCGGGCA TCTTCTTCCA GAGGAACAAG TTTACTTGAA TCAGCAGAGT 
GGTGCCATTC GCTTGGACTG TTTTACTCAT TGCCTAATTG TCAAGTGCAC AGCAGATATT 
TTGCTGTTGG ATACCTTGCT GGGAACCCTT GTAAAAGAAC TCCAAAATAA ATATACCCCT 
GGGCGTAGAG AAGAAGCTGT AGCTGTCACA ATGCGTTTTC TGCGTTCAGT GGCTCGTGTT 
TTTGTCATTC TCAGTGTAGA AATGGCTTCA TCCAAGAAGA AAAACAATTT CATTCCACAG 
CCAATAGGGA AATGCAAGCG AGTGTTCCAA GCTTTACTTC CATATGCAGT GGAGGAGTTG 
TGTAATGTTG CAGAATCCCT CATTGTCCCA GTGAGAATGG GCATTGCACG CCCCACTGCC 
CCCTTCACAT TAGCAAGTAC AAGCATAGAT GCCATGCAGG GCAGCGAGGA GCTGTTCTCT 
GTGGAGCCTC TTCCTCCGAG ACCTTCATCT GATCAGTCTA ACAGTTCGAA TCAGTCCCAG 
TCATCCTATA TCATTAGGAA TCCTCCGCAA AGAAGAATAA GCCAGTCCCA GCCTGTGCGA 
GGCCGAGATG AAGAGCAGGA TGACATTGTA TCAGCAGATG TGGAAGAGGT TGAAGTGGTA 
GAGGGGGTAG CAGGAGAAGA GGACCATCAT GATGAGCAAG AAGAGCATGG GGAGGAGAAT 
ACAGAAGCAG AAGGACAACA TGATGAGCAC GATGAAGATG GTAGTGACAT GGAGTTAGAC 
CTTCTTGCTG CAGCTGAAAC AGAAAGTGAC AGTGAAAGTA ACCACAGCAA TCAGGATAAT 
GCCAGCGGTC GGAGGAGTGT AGTAACAGCA GCTACCGCTG GATCAGAAGC AGGTGCCAGC 
AGCGTTCCGG CCTTTTTCTC TGAAGATGAC TCCCAATCCA ATGACTCCAG TGATTCTGAT 
AGCAGCAGCA GTCAAAGTGA TGACCTGGAC CAGGAGACCT TTATGATTGA TGAGCCTCTA 
GAGAGGTCGT CCAACAGCAA TCATGCAAAT GGGGCTGCCC AGGCTCCAAG GTCAATGCAG 
TGGGCAGTAC GGAACACTCA GAGCCAAAGA GCCCCAAACA CCACACCATC TAATACTTCT 
ACTCCAGCAG CCAGTTCTGG TGCCTTGATT TACATTGAAC CATCCAGTCT GAGGCGCACT 
GGCAACATAA GCTCCAGTGC AGCAGCAGCA GCCGCAGCCC TTGAAGCAAG CAATTCTAGC 
AGTTATCTAA CTTCAGCAAG CAGTCTGGCT AGAGCCTACA GCATAGTGAT ACGGCAGATA 
TCTGACTTGA TGGGTCTAAT CCCCAAGTAC AACCACTTGG TCTATTCACA GATCCCAGCA 
GCCGTCAAAC TGAACTATCA AGATGCCGTT AACTTACAGA ACTATGTTGA AGAAAAACTT 
ATTCCCACAT GGAACTGGAT GGTGAGCATA ATGGACTCCA CTGAAGCCCA GCTCCGTTAT 
GGCTCCGCCT TATCATCCGC TGGAGATCCA GGTCATCCCA ACCATCCTCT TCATGCATCT 
CAGAATTCCA ACCGCAGGGA AAGGATGACT GCCCGTGAGG AAGCTAGTTT GAGGACTCTT 
GAGGGAAGAA GAAGAGCCAC TCTGCTTACT GCTCGACAGG GGATGTCGGC CCGTGGAGAC 
TTCCTGAATT ATGCACTTTC ACTAATGCGT TCGCACAATG ATGAGCACTC TGATGTACTC 
CCTGTTTTGG ATGTTTGCTC CCTGAAACAC GTGGCCTATG TCTTCCAGGC TCTGATATAC 
TGGATTAAGG CAATGAACCA ACAAACAACA TTGGATACTC CACAGCTGGA AAGAAAGAGG 
ACAAGAGAGC TTTTGGAGCT TGGCCTTGAT AATGAAGATT CAGAGCATGA AAATGATGAT 
GACACAAATC AAAGCCTAAT ATTGAATGAC AAAGACGACG ATACACTACC CTCTGAGACC 
GGTCCAAACC ATCCGTTTTT CCGTCGTTCC GATTCAATGA CGTTCCTTGG TTGTATTCCA 
CCAAACCCCT TTGAAGTTCC TCTGGCAGAA GCTATCCCAC TTGCAGACCA GCCCCATCTT 
TTGCAGCCAA ATGCAAGGAA AGAAGACTTA TTTGGTCGAC CGAGTCAAGG TCTCTACTCA 
TCTACTGCTA ACACTGGAAA ATGCCTATTA GAAGTGACAG TTGACAGAAA CTGCCTGGAG 
GTTTTACCAA CCAAAATGTC ATATGCTGCT AATCTAAAAA ATGTAATGAA CATGCAGAGC 
CGTCAGAAAG AAGCAGAGGA ACAGAATGTG CCGGCTGAGG AAAATGACAC ATCAAAGGCT 
GGACCCTCAG CTCATGATCT TGCAGCTCAG CTGAAAAGTA GCTTGTTGGC AGAAATAGGG 
TTAACAGAGA GTGAAGGTCC CCCGCTTACA TCATTCAGGC CACAGTGTAG CTTTATGGGA 
ATGGTCATTT CCCATGATAT GCTGTTAGGA CGTTGGCGAC TATCTCTTGA ATTGTTTGGA 
AGGGTGTTTA TGGAAGATGT CGGAGCAGAG CCTGGATCGA TCTTAACTGA ACTAGGAGGG 
TTTGAAGTAA AGGAATCCAA GTTCCGCAGG GAGATGGAAA AACTTCGAAA TCAGCAGTCC 
AGAGATCTTA CACTAGAGGT GGATCGAGAT AGAGATCTTC TAATACAGCA AACGATGAGG 
CAGCTGAATA ATCATTTTGG GCGCAGATGC GCCACAACTC CTATGGCAGT GCATAGAGTA 
AAGGTCACCT TTAAAGATGA GCCAGGAGAA GGCAGTGGAG TAGCCCGAAG TTTTTATACT 
GCAGTAGCAC AAGCATTTTT GTCCAATGAA AAGTTGCCAA GCTTGGACTG TATCCAAAAT 
GCCAACAAAG GTACTCATAC AAGTTTGATG CAAAGACTCA GAAATCGTGG GGAACGAGAC 
CGGGAAAGGG AGCGGGACAT GAGAAGAAGC AGTGGTTTGC GAACAGGTTC TCGGCGGGAT 
AGAGACAGAG ATTTCAGACG GCAACTTTCC ATTGATACTA GGCCTTTCAG GCCAGCTTCT 
GAGGGCAATC CTAGTGATGA TCCTGATCCT CTTCCTGCTC ACCGACAGGC TCTCGGAGAA 
AGACTATACC CACGTGTACA AGCCATGCAG CCGGCATTTG CAAGTAAAAT TACAGGCATG 
CTGCTTGAGC TATCTCCTGC ACAACTGCTG CTGCTGTTGG CAAGTGAAGA CTCTCTAAGA 
GCCAGAGTGG ATGAAGCTAT GGAACTTATT ATTGCACATG GAAGGGAGAA TGGTGCTGAC 
AGTATCCTCG ATTTAGGTTT ATTAGAAGCA GCTGATAAGG TACAGGAAAA CCGTAAGCGC 
CATGGCTCTA GTCGAAGCGT GGTTGATATG GAGCTTACTG ACACAGACGA TGGGGACGAT 
AATGCTCCTT TATTTTACCA ACCTGGTAAA CGAGGTTTCT ATAGTCCAAG GCCTGGAAAA 
AACACAGAGG CACGTTTAAA CTGTTTCCGC AACATCGGCA GAATTCTTGG TCTGTGCCTA 
CTACAGAATG AATTATGTCC TATCACTTTA AGTAGGCAGG TGATTAAAGT GCTTCTGGGC 
AGAAAGGTGA ACTGGCATGA TTTTGCGTTC TTTGATCACG TGATGTACGA GAGCTTACGG 
CAACTTATAC TCGCCTCCCA AAGCCCGGAT GCTGACGCAG TGTTTGCCTC AATGGACCTA 
GCGTTTGCAA TAGACCTCTG CAAAGAAGAA GGTGGTGGAC AAGTGGAACT TATTCCTAAT 
GGCGTAAATA TCCCTGTAAC CCCTCAAAAT GTGTATGAAT ATGTAAGAAA ATATGCTGAA 
CACAGAATGC TCACTGTAGC AGAGCAACCG CTTAATGCCA TGAGGAAAGG ACTATTGGAT 
GTTCTCCCAA AGAATGCATT AGAAGACTTA ACTGCAGAAG ACTTTAGGCT CTTAGTGAAT 
GGCTGTGGGG AAGTTAATGT TCAGATGCTC ATTAGCTTTA CCTCCTTCAA CGATGAGTCA 
GGAGAAAACG CAGAAAAGCT GTTGCAGTTC AAACGCTGGT TCTGGTCAAT CGTAGAGAAG 
ATGAGTATGA CAGAGCGGCA AGATTTAGTT TATTTTTGGA CCTCAAGCCC ATCATTGCCT 
GCGAGTGAAG AGGGATTCCA GCCCATGCCA TCCATTACCA TCCGGCCCCC CGATGATCAG 
CACCTTCCCA CGGCGAACAC CTGCATCTCT CGCCTCTACG TCCCCCTTTA TTCTTCCAAA 
CAGATCCTGA AACAGAAGTT GTTACTTGCT ATTAAGACCA AGAACTTTGG ATTTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018079384.1
CDS	438..8837
Translation	MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLSSHPPLNVLEQTTIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELSKPENNDGSKLSSGSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGEDLMSLLDADIHSAHPSVIIDADAMFSEDISYFGYPSFRRSSLSRIGSSRVLLLPLERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPTLDKKNTPIMSPVSLGEDLQWWPEKDGTKFISIGALYSELVAVSSKGELYQWKWIEVEPYRNTQNPSLRHPRTSFLGLTNEKIVLLSANSIRATVATESNKVATWVDETLSSVASKLEHTAQIFPELQGDKIVSLHCCALYTCAQLENNLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNLEKTNKTIEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEERVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSNNSNNSNNSNNPCTPGSDPDPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGTWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLTNLPANSTIKKKAAIIIMAVEKQTLMQHILRGDYEACRQYLMNLEQGVVLDPNTQMLQTFLSHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTASTVPGQGPNTSTIPGPSSEPSVVESKDRKANAHFILKLLCDSIVLQPYLRDLLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEANSSEKEEDIFKGMVCPPGTNADDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTITNLVTMPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKAANPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGAIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAVAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSNSSNQSQSSYIIRNPPQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENTEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDLDQETFMIDEPLERSSNSNHANGAAQAPRSMQWAVRNTQSQRAPNTTPSNTSTPAASSGALIYIEPSSLRRTGNISSSAAAAAAALEASNSSSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLNYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALSSAGDPGHPNHPLHASQNSNRRERMTAREEASLRTLEGRRRATLLTARQGMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGLDNEDSEHENDDDTNQSLILNDKDDDTLPSETGPNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSTANTGKCLLEVTVDRNCLEVLPTKMSYAANLKNVMNMQSRQKEAEEQNVPAEENDTSKAGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLTLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAVAQAFLSNEKLPSLDCIQNANKGTHTSLMQRLRNRGERDRERERDMRRSSGLRTGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPDPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLLEAADKVQENRKRHGSSRSVVDMELTDTDDGDDNAPLFYQPGKRGFYSPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLSRQVIKVLLGRKVNWHDFAFFDHVMYESLRQLILASQSPDADAVFASMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLTVAEQPLNAMRKGLLDVLPKNALEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV

Target ORF information:

RefSeq Version	XM_018223895.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018223895.1

ORF Insert Sequence:

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAAGA TCTTATGTCA CTGCTGGATG CTGACATTCA TTCTGCTCAC 
CCAAGTGTCA TAATTGATGC TGATGCTATG TTTTCAGAAG ATATTAGCTA CTTTGGTTAT 
CCATCTTTTC GGCGCTCTTC ACTTTCCAGG ATAGGCTCAT CCAGAGTTCT CCTTCTTCCC 
TTAGAGAGAG ACTCTGAGCT GTTGCGTGAA CGTGAGTCAG TGTTGCGTTT GCGTGAGCGG 
AGGTGGCTTG ATGGGGCCTC GTTTGATAAT GAAAGAGGCT CCACAAGTAA AGAAGGGGAA 
CCTACACTCG ATAAGAAGAA CACTCCCATT ATGAGCCCAG TGTCACTGGG AGAAGACCTG 
CAATGGTGGC CTGAAAAGGA TGGGACAAAG TTTATTTCTA TTGGAGCACT TTATTCCGAG 
CTGGTTGCTG TAAGCAGTAA GGGAGAACTT TATCAGTGGA AGTGGATCGA GGTGGAGCCT 
TATAGGAATA CACAGAATCC ATCATTACGT CATCCAAGAA CATCCTTTTT AGGCCTAACA 
AATGAGAAAA TAGTCCTGTT GTCAGCAAAC AGTATCCGAG CCACAGTCGC TACAGAAAGC 
AACAAGGTTG CCACTTGGGT CGATGAAACA TTAAGCTCGG TAGCCTCAAA GTTAGAACAC 
ACAGCTCAGA TTTTCCCTGA GCTACAAGGA GACAAGATTG TCTCGTTGCA CTGTTGTGCA 
CTTTATACTT GTGCGCAATT AGAAAATAAC CTTTATTGGT GGGGTGTGGT TCCTTTCAGT 
CAGAGGAAGA AAATGCTTGA AAAAGCTCGT GCAAAGAATA AAAAACCCAA ATCTAGTGCC 
GGAATTTCAT CCATGCCAAA TATCACAGTT GGCACCCAGG TATGCTTAAG AAATAACCCT 
CTCTACCATG CGGGAGCTGT TGCTTTCTCG ATCAGTGCTG GCATTCCCAA AGTAGGAGTA 
TTAATGGAAT CTGTGTGGAA TATGAATGAT AGCTGCAGAT TTCAGCTCCG TTCTCCAGAG 
AGTTTAAAAA ACTTGGAAAA AACAAATAAA ACAATTGAAG CCAAACCTGA GAGCAAACAG 
GAACCTGTAA AAACAGAGAT GGGCCCGCCC CCATCTCCCG CATCTACCTG TAGTGATGCC 
TCTTCAATTG CTAGCAGTGC CTCCATGCCA TACAAACGAA GACGATCAAC TCCTGCTCCT 
AAGGAAGAAG AGAGAGTGAA TGAAGAACAG TGGTCTCTCA GAGAAGTTGT TTTTGTAGAA 
GATGTTAAAA ATGTTCCTGT TGGGAAGGTA CTAAAAGTTG ATGGGGCCTA TGTTGCAGTC 
AAATTTCCTG GCACTTCTAA TAATTCTAAT AATTCTAATA ATTCTAACAA TCCGTGCACC 
CCTGGCTCTG ATCCAGACCC ATCCTCTCTC CTCCAGGATT GTAGATTACT ACGGATTGAT 
GAACTACAGG TAGTGAAAAC TGGGGGGACA CCCAAAGTCC CAGACTGTTT CCAAAGGACA 
CCTAAAAAGC TGTGCATTCC TGAAAAAACT GAGATCCTGG CAGTCAATGT AGATTCCAAA 
GGGGTTCACG CGGTACTGAA AACAGGGACA TGGGTACGGT ACTGTATCTT TGATCTTGCA 
ACTGGAAAAG CAGAACAGGA AAACAACTTC CCAACAAGTA GCATTGCATT TCTTGGCCAG 
AATGAGAGAA ATGTTGCTAT TTTTACAGCT GGGCAGGAAT CTCCCATCAT TCTTAGAGAT 
GGCAATGGTA CCATTTATCC CATGGCAAAA GATTGCATGG GTGGGATAAG AGATCCAGAC 
TGGCTTGACC TTCCTCCAAT AAGCAGTCTT GGAATGGGAG TTCATTCTTT AACCAATCTC 
CCTGCAAATT CAACAATTAA GAAGAAAGCA GCCATTATCA TAATGGCTGT TGAGAAGCAA 
ACACTGATGC AACACATTTT GCGCGGTGAT TATGAGGCCT GCAGGCAGTA TTTGATGAAC 
CTAGAGCAAG GTGTAGTTTT GGATCCAAAC ACTCAAATGC TGCAGACTTT TCTGAGCCAC 
AGATGTGATG GCAATAGGAA CATATTGCAT GCCTGCGTGT CCGTTTGCTT CCCAACAAGC 
AACAAGGAAA CAAAAGAAGA GGAGGAAGCA GAGCGCTCTG AAAGGAATAC ATTTGCAGAA 
AGACTGTCTG CAGTAGAAGC AATTGCCAAT GCTATATCTG TGGTTTCTAG CAATGGTCCC 
GGAAATCGTG CTGGATCGTC CAGCAGTAGA AGCTTGAGAT TGAGGGAAAT GATGAGGAGG 
TCCCTTCGGG CAGCAGGTTT AGGAAGACAT GAGGCTGGTG CTTCTAGTGA TCACCAAGAC 
CCAGTATCTC CTCCCATTGC ACCTCCTAGC TGGGTTCCTG ATCCACCTGC TATGGATCCA 
GATGGTGACA TAGATTTTAT CCTGGCCCCT GCTGTAGGAT CTCTTACCAC TGCATCCACT 
GTACCTGGCC AAGGACCAAA TACTTCTACA ATACCAGGTC CTTCCTCAGA GCCATCAGTT 
GTTGAGTCCA AGGATCGGAA GGCAAATGCT CACTTCATAC TGAAGTTATT ATGCGATAGC 
ATCGTTCTGC AGCCTTACCT ACGTGATCTC CTGTCTGCCA AGGATGCAAG AGGTATGACT 
CCCTTCATGT CTGCTGTTAG TGGAAGAGCC TATCCAGCTG CAATTACCAT ACTGGAAACT 
GCACAGAAAA TCGCAAAAGC TGAAGCAAAT TCAAGTGAGA AAGAGGAGGA TATTTTCAAG 
GGAATGGTAT GTCCACCTGG TACCAATGCA GACGACTCTC CTCTATATGT ACTTTGCTGC 
AATGATACCT GCAGTTTTAC ATGGACAGGA GCTGAGCATA TCAATCAGGA CATATTTGAA 
TGTCGAACAT GTGGCTTGTT GGAATCCCTG TGCTGCTGTA CCGAGTGTGC AAGAGTCTGC 
CACAAAGGCC ATGACTGCAA ATTGAAAAGA ACATCACCTA CTGCTTACTG CGACTGTTGG 
GAGAAATGCA AATGCAAAAC ATTAATTGCC GGGCAAAAGT CTGCACGACT TGATCTGCTC 
TATCGACTGC TGACCATCAC TAATCTTGTT ACAATGCCAA ATAGCAGGGG AGAGCACCTA 
CTTCTTTTCT TAGTGCAGAC TGTAGCCAGA CAGACAGTGG AGCACTGCCA GTACCGACCT 
CCAAGGATTC GAGAAGATCG CAATCGAAAG GCAGCTAACC CTGAAGATTC CGACATGCCA 
GATCATGACC TAGAGCCCCC TAGGTTTGCC CAGCTTGCAC TGGAACGTGT TTTACAAGAT 
TGGAACGCAC TCAAATCCAT GATTATGTTT GGATCCCAAG AAAATAAAGA CCCCCTTAGT 
GCTAGCAGTA GAATCGGGCA TCTTCTTCCA GAGGAACAAG TTTACTTGAA TCAGCAGAGT 
GGTGCCATTC GCTTGGACTG TTTTACTCAT TGCCTAATTG TCAAGTGCAC AGCAGATATT 
TTGCTGTTGG ATACCTTGCT GGGAACCCTT GTAAAAGAAC TCCAAAATAA ATATACCCCT 
GGGCGTAGAG AAGAAGCTGT AGCTGTCACA ATGCGTTTTC TGCGTTCAGT GGCTCGTGTT 
TTTGTCATTC TCAGTGTAGA AATGGCTTCA TCCAAGAAGA AAAACAATTT CATTCCACAG 
CCAATAGGGA AATGCAAGCG AGTGTTCCAA GCTTTACTTC CATATGCAGT GGAGGAGTTG 
TGTAATGTTG CAGAATCCCT CATTGTCCCA GTGAGAATGG GCATTGCACG CCCCACTGCC 
CCCTTCACAT TAGCAAGTAC AAGCATAGAT GCCATGCAGG GCAGCGAGGA GCTGTTCTCT 
GTGGAGCCTC TTCCTCCGAG ACCTTCATCT GATCAGTCTA ACAGTTCGAA TCAGTCCCAG 
TCATCCTATA TCATTAGGAA TCCTCCGCAA AGAAGAATAA GCCAGTCCCA GCCTGTGCGA 
GGCCGAGATG AAGAGCAGGA TGACATTGTA TCAGCAGATG TGGAAGAGGT TGAAGTGGTA 
GAGGGGGTAG CAGGAGAAGA GGACCATCAT GATGAGCAAG AAGAGCATGG GGAGGAGAAT 
ACAGAAGCAG AAGGACAACA TGATGAGCAC GATGAAGATG GTAGTGACAT GGAGTTAGAC 
CTTCTTGCTG CAGCTGAAAC AGAAAGTGAC AGTGAAAGTA ACCACAGCAA TCAGGATAAT 
GCCAGCGGTC GGAGGAGTGT AGTAACAGCA GCTACCGCTG GATCAGAAGC AGGTGCCAGC 
AGCGTTCCGG CCTTTTTCTC TGAAGATGAC TCCCAATCCA ATGACTCCAG TGATTCTGAT 
AGCAGCAGCA GTCAAAGTGA TGACCTGGAC CAGGAGACCT TTATGATTGA TGAGCCTCTA 
GAGAGGTCGT CCAACAGCAA TCATGCAAAT GGGGCTGCCC AGGCTCCAAG GTCAATGCAG 
TGGGCAGTAC GGAACACTCA GAGCCAAAGA GCCCCAAACA CCACACCATC TAATACTTCT 
ACTCCAGCAG CCAGTTCTGG TGCCTTGATT TACATTGAAC CATCCAGTCT GAGGCGCACT 
GGCAACATAA GCTCCAGTGC AGCAGCAGCA GCCGCAGCCC TTGAAGCAAG CAATTCTAGC 
AGTTATCTAA CTTCAGCAAG CAGTCTGGCT AGAGCCTACA GCATAGTGAT ACGGCAGATA 
TCTGACTTGA TGGGTCTAAT CCCCAAGTAC AACCACTTGG TCTATTCACA GATCCCAGCA 
GCCGTCAAAC TGAACTATCA AGATGCCGTT AACTTACAGA ACTATGTTGA AGAAAAACTT 
ATTCCCACAT GGAACTGGAT GGTGAGCATA ATGGACTCCA CTGAAGCCCA GCTCCGTTAT 
GGCTCCGCCT TATCATCCGC TGGAGATCCA GGTCATCCCA ACCATCCTCT TCATGCATCT 
CAGAATTCCA ACCGCAGGGA AAGGATGACT GCCCGTGAGG AAGCTAGTTT GAGGACTCTT 
GAGGGAAGAA GAAGAGCCAC TCTGCTTACT GCTCGACAGG GGATGTCGGC CCGTGGAGAC 
TTCCTGAATT ATGCACTTTC ACTAATGCGT TCGCACAATG ATGAGCACTC TGATGTACTC 
CCTGTTTTGG ATGTTTGCTC CCTGAAACAC GTGGCCTATG TCTTCCAGGC TCTGATATAC 
TGGATTAAGG CAATGAACCA ACAAACAACA TTGGATACTC CACAGCTGGA AAGAAAGAGG 
ACAAGAGAGC TTTTGGAGCT TGGCCTTGAT AATGAAGATT CAGAGCATGA AAATGATGAT 
GACACAAATC AAAGCCTAAT ATTGAATGAC AAAGACGACG ATACACTACC CTCTGAGACC 
GGTCCAAACC ATCCGTTTTT CCGTCGTTCC GATTCAATGA CGTTCCTTGG TTGTATTCCA 
CCAAACCCCT TTGAAGTTCC TCTGGCAGAA GCTATCCCAC TTGCAGACCA GCCCCATCTT 
TTGCAGCCAA ATGCAAGGAA AGAAGACTTA TTTGGTCGAC CGAGTCAAGG TCTCTACTCA 
TCTACTGCTA ACACTGGAAA ATGCCTATTA GAAGTGACAG TTGACAGAAA CTGCCTGGAG 
GTTTTACCAA CCAAAATGTC ATATGCTGCT AATCTAAAAA ATGTAATGAA CATGCAGAGC 
CGTCAGAAAG AAGCAGAGGA ACAGAATGTG CCGGCTGAGG AAAATGACAC ATCAAAGGCT 
GGACCCTCAG CTCATGATCT TGCAGCTCAG CTGAAAAGTA GCTTGTTGGC AGAAATAGGG 
TTAACAGAGA GTGAAGGTCC CCCGCTTACA TCATTCAGGC CACAGTGTAG CTTTATGGGA 
ATGGTCATTT CCCATGATAT GCTGTTAGGA CGTTGGCGAC TATCTCTTGA ATTGTTTGGA 
AGGGTGTTTA TGGAAGATGT CGGAGCAGAG CCTGGATCGA TCTTAACTGA ACTAGGAGGG 
TTTGAAGTAA AGGAATCCAA GTTCCGCAGG GAGATGGAAA AACTTCGAAA TCAGCAGTCC 
AGAGATCTTA CACTAGAGGT GGATCGAGAT AGAGATCTTC TAATACAGCA AACGATGAGG 
CAGCTGAATA ATCATTTTGG GCGCAGATGC GCCACAACTC CTATGGCAGT GCATAGAGTA 
AAGGTCACCT TTAAAGATGA GCCAGGAGAA GGCAGTGGAG TAGCCCGAAG TTTTTATACT 
GCAGTAGCAC AAGCATTTTT GTCCAATGAA AAGTTGCCAA GCTTGGACTG TATCCAAAAT 
GCCAACAAAG GTACTCATAC AAGTTTGATG CAAAGACTCA GAAATCGTGG GGAACGAGAC 
CGGGAAAGGG AGCGGGACAT GAGAAGAAGC AGTGGTTTGC GAACAGGTTC TCGGCGGGAT 
AGAGACAGAG ATTTCAGACG GCAACTTTCC ATTGATACTA GGCCTTTCAG GCCAGCTTCT 
GAGGGCAATC CTAGTGATGA TCCTGATCCT CTTCCTGCTC ACCGACAGGC TCTCGGAGAA 
AGACTATACC CACGTGTACA AGCCATGCAG CCGGCATTTG CAAGTAAAAT TACAGGCATG 
CTGCTTGAGC TATCTCCTGC ACAACTGCTG CTGCTGTTGG CAAGTGAAGA CTCTCTAAGA 
GCCAGAGTGG ATGAAGCTAT GGAACTTATT ATTGCACATG GAAGGGAGAA TGGTGCTGAC 
AGTATCCTCG ATTTAGGTTT ATTAGAAGCA GCTGATAAGG TACAGGAAAA CCGTAAGCGC 
CATGGCTCTA GTCGAAGCGT GGTTGATATG GAGCTTACTG ACACAGACGA TGGGGACGAT 
AATGCTCCTT TATTTTACCA ACCTGGTAAA CGAGGTTTCT ATAGTCCAAG GCCTGGAAAA 
AACACAGAGG CACGTTTAAA CTGTTTCCGC AACATCGGCA GAATTCTTGG TCTGTGCCTA 
CTACAGAATG AATTATGTCC TATCACTTTA AGTAGGCAGG TGATTAAAGT GCTTCTGGGC 
AGAAAGGTGA ACTGGCATGA TTTTGCGTTC TTTGATCACG TGATGTACGA GAGCTTACGG 
CAACTTATAC TCGCCTCCCA AAGCCCGGAT GCTGACGCAG TGTTTGCCTC AATGGACCTA 
GCGTTTGCAA TAGACCTCTG CAAAGAAGAA GGTGGTGGAC AAGTGGAACT TATTCCTAAT 
GGCGTAAATA TCCCTGTAAC CCCTCAAAAT GTGTATGAAT ATGTAAGAAA ATATGCTGAA 
CACAGAATGC TCACTGTAGC AGAGCAACCG CTTAATGCCA TGAGGAAAGG ACTATTGGAT 
GTTCTCCCAA AGAATGCATT AGAAGACTTA ACTGCAGAAG ACTTTAGGCT CTTAGTGAAT 
GGCTGTGGGG AAGTTAATGT TCAGATGCTC ATTAGCTTTA CCTCCTTCAA CGATGAGTCA 
GGAGAAAACG CAGAAAAGCT GTTGCAGTTC AAACGCTGGT TCTGGTCAAT CGTAGAGAAG 
ATGAGTATGA CAGAGCGGCA AGATTTAGTT TATTTTTGGA CCTCAAGCCC ATCATTGCCT 
GCGAGTGAAG AGGGATTCCA GCCCATGCCA TCCATTACCA TCCGGCCCCC CGATGATCAG 
CACCTTCCCA CGGCGAACAC CTGCATCTCT CGCCTCTACG TCCCCCTTTA TTCTTCCAAA 
CAGATCCTGA AACAGAAGTT GTTACTTGCT ATTAAGACCA AGAACTTTGG ATTTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108695408 ( XM_018223896.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018079385.1 Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X2, mRNA.

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CloneID	OAf37364
Clone ID Related Accession (Same CDS sequence)	XM_018223896.1
Accession Version	XM_018223896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase UBR5-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAAGA TCTTATGTCA CTGCTGGATG CTGACATTCA TTCTGCTCAC 
CCAAGTGTCA TAATTGATGC TGATGCTATG TTTTCAGAAG ATATTAGCTA CTTTGGTTAT 
CCATCTTTTC GGCGCTCTTC ACTTTCCAGG ATAGGCTCAT CCAGAGTTCT CCTTCTTCCC 
TTAGAGAGAG ACTCTGAGCT GTTGCGTGAA CGTGAGTCAG TGTTGCGTTT GCGTGAGCGG 
AGGTGGCTTG ATGGGGCCTC GTTTGATAAT GAAAGAGGCT CCACAAGTAA AGAAGGGGAA 
CCTACACTCG ATAAGAAGAA CACTCCCATT ATGAGCCCAG TGTCACTGGG AGAAGACCTG 
CAATGGTGGC CTGAAAAGGA TGGGACAAAG TTTATTTCTA TTGGAGCACT TTATTCCGAG 
CTGGTTGCTG TAAGCAGTAA GGGAGAACTT TATCAGTGGA AGTGGATCGA GGTGGAGCCT 
TATAGGAATA CACAGAATCC ATCATTACGT CATCCAAGAA CATCCTTTTT AGGCCTAACA 
AATGAGAAAA TAGTCCTGTT GTCAGCAAAC AGTATCCGAG CCACAGTCGC TACAGAAAGC 
AACAAGGTTG CCACTTGGGT CGATGAAACA TTAAGCTCGG TAGCCTCAAA GTTAGAACAC 
ACAGCTCAGA TTTTCCCTGA GCTACAAGGA GACAAGATTG TCTCGTTGCA CTGTTGTGCA 
CTTTATACTT GTGCGCAATT AGAAAATAAC CTTTATTGGT GGGGTGTGGT TCCTTTCAGT 
CAGAGGAAGA AAATGCTTGA AAAAGCTCGT GCAAAGAATA AAAAACCCAA ATCTAGTGCC 
GGAATTTCAT CCATGCCAAA TATCACAGTT GGCACCCAGG TATGCTTAAG AAATAACCCT 
CTCTACCATG CGGGAGCTGT TGCTTTCTCG ATCAGTGCTG GCATTCCCAA AGTAGGAGTA 
TTAATGGAAT CTGTGTGGAA TATGAATGAT AGCTGCAGAT TTCAGCTCCG TTCTCCAGAG 
AGTTTAAAAA ACTTGGAAAA AACAAATAAA ACAATTGAAG CCAAACCTGA GAGCAAACAG 
GAACCTGTAA AAACAGAGAT GGGCCCGCCC CCATCTCCCG CATCTACCTG TAGTGATGCC 
TCTTCAATTG CTAGCAGTGC CTCCATGCCA TACAAACGAA GACGATCAAC TCCTGCTCCT 
AAGGAAGAAG AGAGAGTGAA TGAAGAACAG TGGTCTCTCA GAGAAGTTGT TTTTGTAGAA 
GATGTTAAAA ATGTTCCTGT TGGGAAGGTA CTAAAAGTTG ATGGGGCCTA TGTTGCAGTC 
AAATTTCCTG GCACTTCTAA TAATTCTAAT AATTCTAATA ATTCTAACAA TCCGTGCACC 
CCTGGCTCTG ATCCAGACCC ATCCTCTCTC CTCCAGGATT GTAGATTACT ACGGATTGAT 
GAACTACAGG TAGTGAAAAC TGGGGGGACA CCCAAAGTCC CAGACTGTTT CCAAAGGACA 
CCTAAAAAGC TGTGCATTCC TGAAAAAACT GAGATCCTGG CAGTCAATGT AGATTCCAAA 
GGGGTTCACG CGGTACTGAA AACAGGGACA TGGGTACGGT ACTGTATCTT TGATCTTGCA 
ACTGGAAAAG CAGAACAGGA AAACAACTTC CCAACAAGTA GCATTGCATT TCTTGGCCAG 
AATGAGAGAA ATGTTGCTAT TTTTACAGCT GGGCAGGAAT CTCCCATCAT TCTTAGAGAT 
GGCAATGGTA CCATTTATCC CATGGCAAAA GATTGCATGG GTGGGATAAG AGATCCAGAC 
TGGCTTGACC TTCCTCCAAT AAGCAGTCTT GGAATGGGAG TTCATTCTTT AACCAATCTC 
CCTGCAAATT CAACAATTAA GAAGAAAGCA GCCATTATCA TAATGGCTGT TGAGAAGCAA 
ACACTGATGC AACACATTTT GCGCGGTGAT TATGAGGCCT GCAGGCAGTA TTTGATGAAC 
CTAGAGCAAG GTGTAGTTTT GGATCCAAAC ACTCAAATGC TGCAGACTTT TCTGAGCCAC 
AGATGTGATG GCAATAGGAA CATATTGCAT GCCTGCGTGT CCGTTTGCTT CCCAACAAGC 
AACAAGGAAA CAAAAGAAGA GGAGGCAGAG CGCTCTGAAA GGAATACATT TGCAGAAAGA 
CTGTCTGCAG TAGAAGCAAT TGCCAATGCT ATATCTGTGG TTTCTAGCAA TGGTCCCGGA 
AATCGTGCTG GATCGTCCAG CAGTAGAAGC TTGAGATTGA GGGAAATGAT GAGGAGGTCC 
CTTCGGGCAG CAGGTTTAGG AAGACATGAG GCTGGTGCTT CTAGTGATCA CCAAGACCCA 
GTATCTCCTC CCATTGCACC TCCTAGCTGG GTTCCTGATC CACCTGCTAT GGATCCAGAT 
GGTGACATAG ATTTTATCCT GGCCCCTGCT GTAGGATCTC TTACCACTGC ATCCACTGTA 
CCTGGCCAAG GACCAAATAC TTCTACAATA CCAGGTCCTT CCTCAGAGCC ATCAGTTGTT 
GAGTCCAAGG ATCGGAAGGC AAATGCTCAC TTCATACTGA AGTTATTATG CGATAGCATC 
GTTCTGCAGC CTTACCTACG TGATCTCCTG TCTGCCAAGG ATGCAAGAGG TATGACTCCC 
TTCATGTCTG CTGTTAGTGG AAGAGCCTAT CCAGCTGCAA TTACCATACT GGAAACTGCA 
CAGAAAATCG CAAAAGCTGA AGCAAATTCA AGTGAGAAAG AGGAGGATAT TTTCAAGGGA 
ATGGTATGTC CACCTGGTAC CAATGCAGAC GACTCTCCTC TATATGTACT TTGCTGCAAT 
GATACCTGCA GTTTTACATG GACAGGAGCT GAGCATATCA ATCAGGACAT ATTTGAATGT 
CGAACATGTG GCTTGTTGGA ATCCCTGTGC TGCTGTACCG AGTGTGCAAG AGTCTGCCAC 
AAAGGCCATG ACTGCAAATT GAAAAGAACA TCACCTACTG CTTACTGCGA CTGTTGGGAG 
AAATGCAAAT GCAAAACATT AATTGCCGGG CAAAAGTCTG CACGACTTGA TCTGCTCTAT 
CGACTGCTGA CCATCACTAA TCTTGTTACA ATGCCAAATA GCAGGGGAGA GCACCTACTT 
CTTTTCTTAG TGCAGACTGT AGCCAGACAG ACAGTGGAGC ACTGCCAGTA CCGACCTCCA 
AGGATTCGAG AAGATCGCAA TCGAAAGGCA GCTAACCCTG AAGATTCCGA CATGCCAGAT 
CATGACCTAG AGCCCCCTAG GTTTGCCCAG CTTGCACTGG AACGTGTTTT ACAAGATTGG 
AACGCACTCA AATCCATGAT TATGTTTGGA TCCCAAGAAA ATAAAGACCC CCTTAGTGCT 
AGCAGTAGAA TCGGGCATCT TCTTCCAGAG GAACAAGTTT ACTTGAATCA GCAGAGTGGT 
GCCATTCGCT TGGACTGTTT TACTCATTGC CTAATTGTCA AGTGCACAGC AGATATTTTG 
CTGTTGGATA CCTTGCTGGG AACCCTTGTA AAAGAACTCC AAAATAAATA TACCCCTGGG 
CGTAGAGAAG AAGCTGTAGC TGTCACAATG CGTTTTCTGC GTTCAGTGGC TCGTGTTTTT 
GTCATTCTCA GTGTAGAAAT GGCTTCATCC AAGAAGAAAA ACAATTTCAT TCCACAGCCA 
ATAGGGAAAT GCAAGCGAGT GTTCCAAGCT TTACTTCCAT ATGCAGTGGA GGAGTTGTGT 
AATGTTGCAG AATCCCTCAT TGTCCCAGTG AGAATGGGCA TTGCACGCCC CACTGCCCCC 
TTCACATTAG CAAGTACAAG CATAGATGCC ATGCAGGGCA GCGAGGAGCT GTTCTCTGTG 
GAGCCTCTTC CTCCGAGACC TTCATCTGAT CAGTCTAACA GTTCGAATCA GTCCCAGTCA 
TCCTATATCA TTAGGAATCC TCCGCAAAGA AGAATAAGCC AGTCCCAGCC TGTGCGAGGC 
CGAGATGAAG AGCAGGATGA CATTGTATCA GCAGATGTGG AAGAGGTTGA AGTGGTAGAG 
GGGGTAGCAG GAGAAGAGGA CCATCATGAT GAGCAAGAAG AGCATGGGGA GGAGAATACA 
GAAGCAGAAG GACAACATGA TGAGCACGAT GAAGATGGTA GTGACATGGA GTTAGACCTT 
CTTGCTGCAG CTGAAACAGA AAGTGACAGT GAAAGTAACC ACAGCAATCA GGATAATGCC 
AGCGGTCGGA GGAGTGTAGT AACAGCAGCT ACCGCTGGAT CAGAAGCAGG TGCCAGCAGC 
GTTCCGGCCT TTTTCTCTGA AGATGACTCC CAATCCAATG ACTCCAGTGA TTCTGATAGC 
AGCAGCAGTC AAAGTGATGA CCTGGACCAG GAGACCTTTA TGATTGATGA GCCTCTAGAG 
AGGTCGTCCA ACAGCAATCA TGCAAATGGG GCTGCCCAGG CTCCAAGGTC AATGCAGTGG 
GCAGTACGGA ACACTCAGAG CCAAAGAGCC CCAAACACCA CACCATCTAA TACTTCTACT 
CCAGCAGCCA GTTCTGGTGC CTTGATTTAC ATTGAACCAT CCAGTCTGAG GCGCACTGGC 
AACATAAGCT CCAGTGCAGC AGCAGCAGCC GCAGCCCTTG AAGCAAGCAA TTCTAGCAGT 
TATCTAACTT CAGCAAGCAG TCTGGCTAGA GCCTACAGCA TAGTGATACG GCAGATATCT 
GACTTGATGG GTCTAATCCC CAAGTACAAC CACTTGGTCT ATTCACAGAT CCCAGCAGCC 
GTCAAACTGA ACTATCAAGA TGCCGTTAAC TTACAGAACT ATGTTGAAGA AAAACTTATT 
CCCACATGGA ACTGGATGGT GAGCATAATG GACTCCACTG AAGCCCAGCT CCGTTATGGC 
TCCGCCTTAT CATCCGCTGG AGATCCAGGT CATCCCAACC ATCCTCTTCA TGCATCTCAG 
AATTCCAACC GCAGGGAAAG GATGACTGCC CGTGAGGAAG CTAGTTTGAG GACTCTTGAG 
GGAAGAAGAA GAGCCACTCT GCTTACTGCT CGACAGGGGA TGTCGGCCCG TGGAGACTTC 
CTGAATTATG CACTTTCACT AATGCGTTCG CACAATGATG AGCACTCTGA TGTACTCCCT 
GTTTTGGATG TTTGCTCCCT GAAACACGTG GCCTATGTCT TCCAGGCTCT GATATACTGG 
ATTAAGGCAA TGAACCAACA AACAACATTG GATACTCCAC AGCTGGAAAG AAAGAGGACA 
AGAGAGCTTT TGGAGCTTGG CCTTGATAAT GAAGATTCAG AGCATGAAAA TGATGATGAC 
ACAAATCAAA GCCTAATATT GAATGACAAA GACGACGATA CACTACCCTC TGAGACCGGT 
CCAAACCATC CGTTTTTCCG TCGTTCCGAT TCAATGACGT TCCTTGGTTG TATTCCACCA 
AACCCCTTTG AAGTTCCTCT GGCAGAAGCT ATCCCACTTG CAGACCAGCC CCATCTTTTG 
CAGCCAAATG CAAGGAAAGA AGACTTATTT GGTCGACCGA GTCAAGGTCT CTACTCATCT 
ACTGCTAACA CTGGAAAATG CCTATTAGAA GTGACAGTTG ACAGAAACTG CCTGGAGGTT 
TTACCAACCA AAATGTCATA TGCTGCTAAT CTAAAAAATG TAATGAACAT GCAGAGCCGT 
CAGAAAGAAG CAGAGGAACA GAATGTGCCG GCTGAGGAAA ATGACACATC AAAGGCTGGA 
CCCTCAGCTC ATGATCTTGC AGCTCAGCTG AAAAGTAGCT TGTTGGCAGA AATAGGGTTA 
ACAGAGAGTG AAGGTCCCCC GCTTACATCA TTCAGGCCAC AGTGTAGCTT TATGGGAATG 
GTCATTTCCC ATGATATGCT GTTAGGACGT TGGCGACTAT CTCTTGAATT GTTTGGAAGG 
GTGTTTATGG AAGATGTCGG AGCAGAGCCT GGATCGATCT TAACTGAACT AGGAGGGTTT 
GAAGTAAAGG AATCCAAGTT CCGCAGGGAG ATGGAAAAAC TTCGAAATCA GCAGTCCAGA 
GATCTTACAC TAGAGGTGGA TCGAGATAGA GATCTTCTAA TACAGCAAAC GATGAGGCAG 
CTGAATAATC ATTTTGGGCG CAGATGCGCC ACAACTCCTA TGGCAGTGCA TAGAGTAAAG 
GTCACCTTTA AAGATGAGCC AGGAGAAGGC AGTGGAGTAG CCCGAAGTTT TTATACTGCA 
GTAGCACAAG CATTTTTGTC CAATGAAAAG TTGCCAAGCT TGGACTGTAT CCAAAATGCC 
AACAAAGGTA CTCATACAAG TTTGATGCAA AGACTCAGAA ATCGTGGGGA ACGAGACCGG 
GAAAGGGAGC GGGACATGAG AAGAAGCAGT GGTTTGCGAA CAGGTTCTCG GCGGGATAGA 
GACAGAGATT TCAGACGGCA ACTTTCCATT GATACTAGGC CTTTCAGGCC AGCTTCTGAG 
GGCAATCCTA GTGATGATCC TGATCCTCTT CCTGCTCACC GACAGGCTCT CGGAGAAAGA 
CTATACCCAC GTGTACAAGC CATGCAGCCG GCATTTGCAA GTAAAATTAC AGGCATGCTG 
CTTGAGCTAT CTCCTGCACA ACTGCTGCTG CTGTTGGCAA GTGAAGACTC TCTAAGAGCC 
AGAGTGGATG AAGCTATGGA ACTTATTATT GCACATGGAA GGGAGAATGG TGCTGACAGT 
ATCCTCGATT TAGGTTTATT AGAAGCAGCT GATAAGGTAC AGGAAAACCG TAAGCGCCAT 
GGCTCTAGTC GAAGCGTGGT TGATATGGAG CTTACTGACA CAGACGATGG GGACGATAAT 
GCTCCTTTAT TTTACCAACC TGGTAAACGA GGTTTCTATA GTCCAAGGCC TGGAAAAAAC 
ACAGAGGCAC GTTTAAACTG TTTCCGCAAC ATCGGCAGAA TTCTTGGTCT GTGCCTACTA 
CAGAATGAAT TATGTCCTAT CACTTTAAGT AGGCAGGTGA TTAAAGTGCT TCTGGGCAGA 
AAGGTGAACT GGCATGATTT TGCGTTCTTT GATCACGTGA TGTACGAGAG CTTACGGCAA 
CTTATACTCG CCTCCCAAAG CCCGGATGCT GACGCAGTGT TTGCCTCAAT GGACCTAGCG 
TTTGCAATAG ACCTCTGCAA AGAAGAAGGT GGTGGACAAG TGGAACTTAT TCCTAATGGC 
GTAAATATCC CTGTAACCCC TCAAAATGTG TATGAATATG TAAGAAAATA TGCTGAACAC 
AGAATGCTCA CTGTAGCAGA GCAACCGCTT AATGCCATGA GGAAAGGACT ATTGGATGTT 
CTCCCAAAGA ATGCATTAGA AGACTTAACT GCAGAAGACT TTAGGCTCTT AGTGAATGGC 
TGTGGGGAAG TTAATGTTCA GATGCTCATT AGCTTTACCT CCTTCAACGA TGAGTCAGGA 
GAAAACGCAG AAAAGCTGTT GCAGTTCAAA CGCTGGTTCT GGTCAATCGT AGAGAAGATG 
AGTATGACAG AGCGGCAAGA TTTAGTTTAT TTTTGGACCT CAAGCCCATC ATTGCCTGCG 
AGTGAAGAGG GATTCCAGCC CATGCCATCC ATTACCATCC GGCCCCCCGA TGATCAGCAC 
CTTCCCACGG CGAACACCTG CATCTCTCGC CTCTACGTCC CCCTTTATTC TTCCAAACAG 
ATCCTGAAAC AGAAGTTGTT ACTTGCTATT AAGACCAAGA ACTTTGGATT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018079385.1
CDS	438..8834
Translation	MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLSSHPPLNVLEQTTIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELSKPENNDGSKLSSGSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGEDLMSLLDADIHSAHPSVIIDADAMFSEDISYFGYPSFRRSSLSRIGSSRVLLLPLERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPTLDKKNTPIMSPVSLGEDLQWWPEKDGTKFISIGALYSELVAVSSKGELYQWKWIEVEPYRNTQNPSLRHPRTSFLGLTNEKIVLLSANSIRATVATESNKVATWVDETLSSVASKLEHTAQIFPELQGDKIVSLHCCALYTCAQLENNLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNLEKTNKTIEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEERVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSNNSNNSNNSNNPCTPGSDPDPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGTWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLTNLPANSTIKKKAAIIIMAVEKQTLMQHILRGDYEACRQYLMNLEQGVVLDPNTQMLQTFLSHRCDGNRNILHACVSVCFPTSNKETKEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTASTVPGQGPNTSTIPGPSSEPSVVESKDRKANAHFILKLLCDSIVLQPYLRDLLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEANSSEKEEDIFKGMVCPPGTNADDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTITNLVTMPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKAANPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGAIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAVAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSNSSNQSQSSYIIRNPPQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENTEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDLDQETFMIDEPLERSSNSNHANGAAQAPRSMQWAVRNTQSQRAPNTTPSNTSTPAASSGALIYIEPSSLRRTGNISSSAAAAAAALEASNSSSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLNYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALSSAGDPGHPNHPLHASQNSNRRERMTAREEASLRTLEGRRRATLLTARQGMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGLDNEDSEHENDDDTNQSLILNDKDDDTLPSETGPNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSTANTGKCLLEVTVDRNCLEVLPTKMSYAANLKNVMNMQSRQKEAEEQNVPAEENDTSKAGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLTLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAVAQAFLSNEKLPSLDCIQNANKGTHTSLMQRLRNRGERDRERERDMRRSSGLRTGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPDPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLLEAADKVQENRKRHGSSRSVVDMELTDTDDGDDNAPLFYQPGKRGFYSPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLSRQVIKVLLGRKVNWHDFAFFDHVMYESLRQLILASQSPDADAVFASMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLTVAEQPLNAMRKGLLDVLPKNALEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV

Target ORF information:

RefSeq Version	XM_018223896.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018223896.1

ORF Insert Sequence:

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAAGA TCTTATGTCA CTGCTGGATG CTGACATTCA TTCTGCTCAC 
CCAAGTGTCA TAATTGATGC TGATGCTATG TTTTCAGAAG ATATTAGCTA CTTTGGTTAT 
CCATCTTTTC GGCGCTCTTC ACTTTCCAGG ATAGGCTCAT CCAGAGTTCT CCTTCTTCCC 
TTAGAGAGAG ACTCTGAGCT GTTGCGTGAA CGTGAGTCAG TGTTGCGTTT GCGTGAGCGG 
AGGTGGCTTG ATGGGGCCTC GTTTGATAAT GAAAGAGGCT CCACAAGTAA AGAAGGGGAA 
CCTACACTCG ATAAGAAGAA CACTCCCATT ATGAGCCCAG TGTCACTGGG AGAAGACCTG 
CAATGGTGGC CTGAAAAGGA TGGGACAAAG TTTATTTCTA TTGGAGCACT TTATTCCGAG 
CTGGTTGCTG TAAGCAGTAA GGGAGAACTT TATCAGTGGA AGTGGATCGA GGTGGAGCCT 
TATAGGAATA CACAGAATCC ATCATTACGT CATCCAAGAA CATCCTTTTT AGGCCTAACA 
AATGAGAAAA TAGTCCTGTT GTCAGCAAAC AGTATCCGAG CCACAGTCGC TACAGAAAGC 
AACAAGGTTG CCACTTGGGT CGATGAAACA TTAAGCTCGG TAGCCTCAAA GTTAGAACAC 
ACAGCTCAGA TTTTCCCTGA GCTACAAGGA GACAAGATTG TCTCGTTGCA CTGTTGTGCA 
CTTTATACTT GTGCGCAATT AGAAAATAAC CTTTATTGGT GGGGTGTGGT TCCTTTCAGT 
CAGAGGAAGA AAATGCTTGA AAAAGCTCGT GCAAAGAATA AAAAACCCAA ATCTAGTGCC 
GGAATTTCAT CCATGCCAAA TATCACAGTT GGCACCCAGG TATGCTTAAG AAATAACCCT 
CTCTACCATG CGGGAGCTGT TGCTTTCTCG ATCAGTGCTG GCATTCCCAA AGTAGGAGTA 
TTAATGGAAT CTGTGTGGAA TATGAATGAT AGCTGCAGAT TTCAGCTCCG TTCTCCAGAG 
AGTTTAAAAA ACTTGGAAAA AACAAATAAA ACAATTGAAG CCAAACCTGA GAGCAAACAG 
GAACCTGTAA AAACAGAGAT GGGCCCGCCC CCATCTCCCG CATCTACCTG TAGTGATGCC 
TCTTCAATTG CTAGCAGTGC CTCCATGCCA TACAAACGAA GACGATCAAC TCCTGCTCCT 
AAGGAAGAAG AGAGAGTGAA TGAAGAACAG TGGTCTCTCA GAGAAGTTGT TTTTGTAGAA 
GATGTTAAAA ATGTTCCTGT TGGGAAGGTA CTAAAAGTTG ATGGGGCCTA TGTTGCAGTC 
AAATTTCCTG GCACTTCTAA TAATTCTAAT AATTCTAATA ATTCTAACAA TCCGTGCACC 
CCTGGCTCTG ATCCAGACCC ATCCTCTCTC CTCCAGGATT GTAGATTACT ACGGATTGAT 
GAACTACAGG TAGTGAAAAC TGGGGGGACA CCCAAAGTCC CAGACTGTTT CCAAAGGACA 
CCTAAAAAGC TGTGCATTCC TGAAAAAACT GAGATCCTGG CAGTCAATGT AGATTCCAAA 
GGGGTTCACG CGGTACTGAA AACAGGGACA TGGGTACGGT ACTGTATCTT TGATCTTGCA 
ACTGGAAAAG CAGAACAGGA AAACAACTTC CCAACAAGTA GCATTGCATT TCTTGGCCAG 
AATGAGAGAA ATGTTGCTAT TTTTACAGCT GGGCAGGAAT CTCCCATCAT TCTTAGAGAT 
GGCAATGGTA CCATTTATCC CATGGCAAAA GATTGCATGG GTGGGATAAG AGATCCAGAC 
TGGCTTGACC TTCCTCCAAT AAGCAGTCTT GGAATGGGAG TTCATTCTTT AACCAATCTC 
CCTGCAAATT CAACAATTAA GAAGAAAGCA GCCATTATCA TAATGGCTGT TGAGAAGCAA 
ACACTGATGC AACACATTTT GCGCGGTGAT TATGAGGCCT GCAGGCAGTA TTTGATGAAC 
CTAGAGCAAG GTGTAGTTTT GGATCCAAAC ACTCAAATGC TGCAGACTTT TCTGAGCCAC 
AGATGTGATG GCAATAGGAA CATATTGCAT GCCTGCGTGT CCGTTTGCTT CCCAACAAGC 
AACAAGGAAA CAAAAGAAGA GGAGGCAGAG CGCTCTGAAA GGAATACATT TGCAGAAAGA 
CTGTCTGCAG TAGAAGCAAT TGCCAATGCT ATATCTGTGG TTTCTAGCAA TGGTCCCGGA 
AATCGTGCTG GATCGTCCAG CAGTAGAAGC TTGAGATTGA GGGAAATGAT GAGGAGGTCC 
CTTCGGGCAG CAGGTTTAGG AAGACATGAG GCTGGTGCTT CTAGTGATCA CCAAGACCCA 
GTATCTCCTC CCATTGCACC TCCTAGCTGG GTTCCTGATC CACCTGCTAT GGATCCAGAT 
GGTGACATAG ATTTTATCCT GGCCCCTGCT GTAGGATCTC TTACCACTGC ATCCACTGTA 
CCTGGCCAAG GACCAAATAC TTCTACAATA CCAGGTCCTT CCTCAGAGCC ATCAGTTGTT 
GAGTCCAAGG ATCGGAAGGC AAATGCTCAC TTCATACTGA AGTTATTATG CGATAGCATC 
GTTCTGCAGC CTTACCTACG TGATCTCCTG TCTGCCAAGG ATGCAAGAGG TATGACTCCC 
TTCATGTCTG CTGTTAGTGG AAGAGCCTAT CCAGCTGCAA TTACCATACT GGAAACTGCA 
CAGAAAATCG CAAAAGCTGA AGCAAATTCA AGTGAGAAAG AGGAGGATAT TTTCAAGGGA 
ATGGTATGTC CACCTGGTAC CAATGCAGAC GACTCTCCTC TATATGTACT TTGCTGCAAT 
GATACCTGCA GTTTTACATG GACAGGAGCT GAGCATATCA ATCAGGACAT ATTTGAATGT 
CGAACATGTG GCTTGTTGGA ATCCCTGTGC TGCTGTACCG AGTGTGCAAG AGTCTGCCAC 
AAAGGCCATG ACTGCAAATT GAAAAGAACA TCACCTACTG CTTACTGCGA CTGTTGGGAG 
AAATGCAAAT GCAAAACATT AATTGCCGGG CAAAAGTCTG CACGACTTGA TCTGCTCTAT 
CGACTGCTGA CCATCACTAA TCTTGTTACA ATGCCAAATA GCAGGGGAGA GCACCTACTT 
CTTTTCTTAG TGCAGACTGT AGCCAGACAG ACAGTGGAGC ACTGCCAGTA CCGACCTCCA 
AGGATTCGAG AAGATCGCAA TCGAAAGGCA GCTAACCCTG AAGATTCCGA CATGCCAGAT 
CATGACCTAG AGCCCCCTAG GTTTGCCCAG CTTGCACTGG AACGTGTTTT ACAAGATTGG 
AACGCACTCA AATCCATGAT TATGTTTGGA TCCCAAGAAA ATAAAGACCC CCTTAGTGCT 
AGCAGTAGAA TCGGGCATCT TCTTCCAGAG GAACAAGTTT ACTTGAATCA GCAGAGTGGT 
GCCATTCGCT TGGACTGTTT TACTCATTGC CTAATTGTCA AGTGCACAGC AGATATTTTG 
CTGTTGGATA CCTTGCTGGG AACCCTTGTA AAAGAACTCC AAAATAAATA TACCCCTGGG 
CGTAGAGAAG AAGCTGTAGC TGTCACAATG CGTTTTCTGC GTTCAGTGGC TCGTGTTTTT 
GTCATTCTCA GTGTAGAAAT GGCTTCATCC AAGAAGAAAA ACAATTTCAT TCCACAGCCA 
ATAGGGAAAT GCAAGCGAGT GTTCCAAGCT TTACTTCCAT ATGCAGTGGA GGAGTTGTGT 
AATGTTGCAG AATCCCTCAT TGTCCCAGTG AGAATGGGCA TTGCACGCCC CACTGCCCCC 
TTCACATTAG CAAGTACAAG CATAGATGCC ATGCAGGGCA GCGAGGAGCT GTTCTCTGTG 
GAGCCTCTTC CTCCGAGACC TTCATCTGAT CAGTCTAACA GTTCGAATCA GTCCCAGTCA 
TCCTATATCA TTAGGAATCC TCCGCAAAGA AGAATAAGCC AGTCCCAGCC TGTGCGAGGC 
CGAGATGAAG AGCAGGATGA CATTGTATCA GCAGATGTGG AAGAGGTTGA AGTGGTAGAG 
GGGGTAGCAG GAGAAGAGGA CCATCATGAT GAGCAAGAAG AGCATGGGGA GGAGAATACA 
GAAGCAGAAG GACAACATGA TGAGCACGAT GAAGATGGTA GTGACATGGA GTTAGACCTT 
CTTGCTGCAG CTGAAACAGA AAGTGACAGT GAAAGTAACC ACAGCAATCA GGATAATGCC 
AGCGGTCGGA GGAGTGTAGT AACAGCAGCT ACCGCTGGAT CAGAAGCAGG TGCCAGCAGC 
GTTCCGGCCT TTTTCTCTGA AGATGACTCC CAATCCAATG ACTCCAGTGA TTCTGATAGC 
AGCAGCAGTC AAAGTGATGA CCTGGACCAG GAGACCTTTA TGATTGATGA GCCTCTAGAG 
AGGTCGTCCA ACAGCAATCA TGCAAATGGG GCTGCCCAGG CTCCAAGGTC AATGCAGTGG 
GCAGTACGGA ACACTCAGAG CCAAAGAGCC CCAAACACCA CACCATCTAA TACTTCTACT 
CCAGCAGCCA GTTCTGGTGC CTTGATTTAC ATTGAACCAT CCAGTCTGAG GCGCACTGGC 
AACATAAGCT CCAGTGCAGC AGCAGCAGCC GCAGCCCTTG AAGCAAGCAA TTCTAGCAGT 
TATCTAACTT CAGCAAGCAG TCTGGCTAGA GCCTACAGCA TAGTGATACG GCAGATATCT 
GACTTGATGG GTCTAATCCC CAAGTACAAC CACTTGGTCT ATTCACAGAT CCCAGCAGCC 
GTCAAACTGA ACTATCAAGA TGCCGTTAAC TTACAGAACT ATGTTGAAGA AAAACTTATT 
CCCACATGGA ACTGGATGGT GAGCATAATG GACTCCACTG AAGCCCAGCT CCGTTATGGC 
TCCGCCTTAT CATCCGCTGG AGATCCAGGT CATCCCAACC ATCCTCTTCA TGCATCTCAG 
AATTCCAACC GCAGGGAAAG GATGACTGCC CGTGAGGAAG CTAGTTTGAG GACTCTTGAG 
GGAAGAAGAA GAGCCACTCT GCTTACTGCT CGACAGGGGA TGTCGGCCCG TGGAGACTTC 
CTGAATTATG CACTTTCACT AATGCGTTCG CACAATGATG AGCACTCTGA TGTACTCCCT 
GTTTTGGATG TTTGCTCCCT GAAACACGTG GCCTATGTCT TCCAGGCTCT GATATACTGG 
ATTAAGGCAA TGAACCAACA AACAACATTG GATACTCCAC AGCTGGAAAG AAAGAGGACA 
AGAGAGCTTT TGGAGCTTGG CCTTGATAAT GAAGATTCAG AGCATGAAAA TGATGATGAC 
ACAAATCAAA GCCTAATATT GAATGACAAA GACGACGATA CACTACCCTC TGAGACCGGT 
CCAAACCATC CGTTTTTCCG TCGTTCCGAT TCAATGACGT TCCTTGGTTG TATTCCACCA 
AACCCCTTTG AAGTTCCTCT GGCAGAAGCT ATCCCACTTG CAGACCAGCC CCATCTTTTG 
CAGCCAAATG CAAGGAAAGA AGACTTATTT GGTCGACCGA GTCAAGGTCT CTACTCATCT 
ACTGCTAACA CTGGAAAATG CCTATTAGAA GTGACAGTTG ACAGAAACTG CCTGGAGGTT 
TTACCAACCA AAATGTCATA TGCTGCTAAT CTAAAAAATG TAATGAACAT GCAGAGCCGT 
CAGAAAGAAG CAGAGGAACA GAATGTGCCG GCTGAGGAAA ATGACACATC AAAGGCTGGA 
CCCTCAGCTC ATGATCTTGC AGCTCAGCTG AAAAGTAGCT TGTTGGCAGA AATAGGGTTA 
ACAGAGAGTG AAGGTCCCCC GCTTACATCA TTCAGGCCAC AGTGTAGCTT TATGGGAATG 
GTCATTTCCC ATGATATGCT GTTAGGACGT TGGCGACTAT CTCTTGAATT GTTTGGAAGG 
GTGTTTATGG AAGATGTCGG AGCAGAGCCT GGATCGATCT TAACTGAACT AGGAGGGTTT 
GAAGTAAAGG AATCCAAGTT CCGCAGGGAG ATGGAAAAAC TTCGAAATCA GCAGTCCAGA 
GATCTTACAC TAGAGGTGGA TCGAGATAGA GATCTTCTAA TACAGCAAAC GATGAGGCAG 
CTGAATAATC ATTTTGGGCG CAGATGCGCC ACAACTCCTA TGGCAGTGCA TAGAGTAAAG 
GTCACCTTTA AAGATGAGCC AGGAGAAGGC AGTGGAGTAG CCCGAAGTTT TTATACTGCA 
GTAGCACAAG CATTTTTGTC CAATGAAAAG TTGCCAAGCT TGGACTGTAT CCAAAATGCC 
AACAAAGGTA CTCATACAAG TTTGATGCAA AGACTCAGAA ATCGTGGGGA ACGAGACCGG 
GAAAGGGAGC GGGACATGAG AAGAAGCAGT GGTTTGCGAA CAGGTTCTCG GCGGGATAGA 
GACAGAGATT TCAGACGGCA ACTTTCCATT GATACTAGGC CTTTCAGGCC AGCTTCTGAG 
GGCAATCCTA GTGATGATCC TGATCCTCTT CCTGCTCACC GACAGGCTCT CGGAGAAAGA 
CTATACCCAC GTGTACAAGC CATGCAGCCG GCATTTGCAA GTAAAATTAC AGGCATGCTG 
CTTGAGCTAT CTCCTGCACA ACTGCTGCTG CTGTTGGCAA GTGAAGACTC TCTAAGAGCC 
AGAGTGGATG AAGCTATGGA ACTTATTATT GCACATGGAA GGGAGAATGG TGCTGACAGT 
ATCCTCGATT TAGGTTTATT AGAAGCAGCT GATAAGGTAC AGGAAAACCG TAAGCGCCAT 
GGCTCTAGTC GAAGCGTGGT TGATATGGAG CTTACTGACA CAGACGATGG GGACGATAAT 
GCTCCTTTAT TTTACCAACC TGGTAAACGA GGTTTCTATA GTCCAAGGCC TGGAAAAAAC 
ACAGAGGCAC GTTTAAACTG TTTCCGCAAC ATCGGCAGAA TTCTTGGTCT GTGCCTACTA 
CAGAATGAAT TATGTCCTAT CACTTTAAGT AGGCAGGTGA TTAAAGTGCT TCTGGGCAGA 
AAGGTGAACT GGCATGATTT TGCGTTCTTT GATCACGTGA TGTACGAGAG CTTACGGCAA 
CTTATACTCG CCTCCCAAAG CCCGGATGCT GACGCAGTGT TTGCCTCAAT GGACCTAGCG 
TTTGCAATAG ACCTCTGCAA AGAAGAAGGT GGTGGACAAG TGGAACTTAT TCCTAATGGC 
GTAAATATCC CTGTAACCCC TCAAAATGTG TATGAATATG TAAGAAAATA TGCTGAACAC 
AGAATGCTCA CTGTAGCAGA GCAACCGCTT AATGCCATGA GGAAAGGACT ATTGGATGTT 
CTCCCAAAGA ATGCATTAGA AGACTTAACT GCAGAAGACT TTAGGCTCTT AGTGAATGGC 
TGTGGGGAAG TTAATGTTCA GATGCTCATT AGCTTTACCT CCTTCAACGA TGAGTCAGGA 
GAAAACGCAG AAAAGCTGTT GCAGTTCAAA CGCTGGTTCT GGTCAATCGT AGAGAAGATG 
AGTATGACAG AGCGGCAAGA TTTAGTTTAT TTTTGGACCT CAAGCCCATC ATTGCCTGCG 
AGTGAAGAGG GATTCCAGCC CATGCCATCC ATTACCATCC GGCCCCCCGA TGATCAGCAC 
CTTCCCACGG CGAACACCTG CATCTCTCGC CTCTACGTCC CCCTTTATTC TTCCAAACAG 
ATCCTGAAAC AGAAGTTGTT ACTTGCTATT AAGACCAAGA ACTTTGGATT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108695408 ( XM_018223897.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018079386.1 Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X3, mRNA.

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CloneID	OAf37365
Clone ID Related Accession (Same CDS sequence)	XM_018223897.1
Accession Version	XM_018223897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase UBR5-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGCAGG TGGAGTTCTG GTGTTGGTGG AAGTGGTGGG 
GGATCCTCTG GGCGGTCATC AGCTGGTGCT AGAGACTCTC GGAGGCAAAC AAGAGTTATA 
CGCACAGGAA GGGACAGGGG CTCTGGTCTC CTGGGCAGTC AACCACAGCC TGTGATTCCA 
GCTTCTGTTA TTCCTGAGGA GCTCATCTCA CAGGCACAAG TTGTTCTTCA AGGAAAATCA 
CGAAGTGTTA TCATTCGAGA GCTGCAGAGA ACAAATCTTG ATGTGAACCT TGCAGTAAAC 
AATCTCTTGA GTCGAGATGA TGAAGATGGG GATGATGGTG ATGACACTGC TAGTGAATCT 
TACTTGCCTG GAGAAGATCT TATGTCACTG CTGGATGCTG ACATTCATTC TGCTCACCCA 
AGTGTCATAA TTGATGCTGA TGCTATGTTT TCAGAAGATA TTAGCTACTT TGGTTATCCA 
TCTTTTCGGC GCTCTTCACT TTCCAGGATA GGCTCATCCA GAGTTCTCCT TCTTCCCTTA 
GAGAGAGACT CTGAGCTGTT GCGTGAACGT GAGTCAGTGT TGCGTTTGCG TGAGCGGAGG 
TGGCTTGATG GGGCCTCGTT TGATAATGAA AGAGGCTCCA CAAGTAAAGA AGGGGAACCT 
ACACTCGATA AGAAGAACAC TCCCATTATG AGCCCAGTGT CACTGGGAGA AGACCTGCAA 
TGGTGGCCTG AAAAGGATGG GACAAAGTTT ATTTCTATTG GAGCACTTTA TTCCGAGCTG 
GTTGCTGTAA GCAGTAAGGG AGAACTTTAT CAGTGGAAGT GGATCGAGGT GGAGCCTTAT 
AGGAATACAC AGAATCCATC ATTACGTCAT CCAAGAACAT CCTTTTTAGG CCTAACAAAT 
GAGAAAATAG TCCTGTTGTC AGCAAACAGT ATCCGAGCCA CAGTCGCTAC AGAAAGCAAC 
AAGGTTGCCA CTTGGGTCGA TGAAACATTA AGCTCGGTAG CCTCAAAGTT AGAACACACA 
GCTCAGATTT TCCCTGAGCT ACAAGGAGAC AAGATTGTCT CGTTGCACTG TTGTGCACTT 
TATACTTGTG CGCAATTAGA AAATAACCTT TATTGGTGGG GTGTGGTTCC TTTCAGTCAG 
AGGAAGAAAA TGCTTGAAAA AGCTCGTGCA AAGAATAAAA AACCCAAATC TAGTGCCGGA 
ATTTCATCCA TGCCAAATAT CACAGTTGGC ACCCAGGTAT GCTTAAGAAA TAACCCTCTC 
TACCATGCGG GAGCTGTTGC TTTCTCGATC AGTGCTGGCA TTCCCAAAGT AGGAGTATTA 
ATGGAATCTG TGTGGAATAT GAATGATAGC TGCAGATTTC AGCTCCGTTC TCCAGAGAGT 
TTAAAAAACT TGGAAAAAAC AAATAAAACA ATTGAAGCCA AACCTGAGAG CAAACAGGAA 
CCTGTAAAAA CAGAGATGGG CCCGCCCCCA TCTCCCGCAT CTACCTGTAG TGATGCCTCT 
TCAATTGCTA GCAGTGCCTC CATGCCATAC AAACGAAGAC GATCAACTCC TGCTCCTAAG 
GAAGAAGAGA GAGTGAATGA AGAACAGTGG TCTCTCAGAG AAGTTGTTTT TGTAGAAGAT 
GTTAAAAATG TTCCTGTTGG GAAGGTACTA AAAGTTGATG GGGCCTATGT TGCAGTCAAA 
TTTCCTGGCA CTTCTAATAA TTCTAATAAT TCTAATAATT CTAACAATCC GTGCACCCCT 
GGCTCTGATC CAGACCCATC CTCTCTCCTC CAGGATTGTA GATTACTACG GATTGATGAA 
CTACAGGTAG TGAAAACTGG GGGGACACCC AAAGTCCCAG ACTGTTTCCA AAGGACACCT 
AAAAAGCTGT GCATTCCTGA AAAAACTGAG ATCCTGGCAG TCAATGTAGA TTCCAAAGGG 
GTTCACGCGG TACTGAAAAC AGGGACATGG GTACGGTACT GTATCTTTGA TCTTGCAACT 
GGAAAAGCAG AACAGGAAAA CAACTTCCCA ACAAGTAGCA TTGCATTTCT TGGCCAGAAT 
GAGAGAAATG TTGCTATTTT TACAGCTGGG CAGGAATCTC CCATCATTCT TAGAGATGGC 
AATGGTACCA TTTATCCCAT GGCAAAAGAT TGCATGGGTG GGATAAGAGA TCCAGACTGG 
CTTGACCTTC CTCCAATAAG CAGTCTTGGA ATGGGAGTTC ATTCTTTAAC CAATCTCCCT 
GCAAATTCAA CAATTAAGAA GAAAGCAGCC ATTATCATAA TGGCTGTTGA GAAGCAAACA 
CTGATGCAAC ACATTTTGCG CGGTGATTAT GAGGCCTGCA GGCAGTATTT GATGAACCTA 
GAGCAAGGTG TAGTTTTGGA TCCAAACACT CAAATGCTGC AGACTTTTCT GAGCCACAGA 
TGTGATGGCA ATAGGAACAT ATTGCATGCC TGCGTGTCCG TTTGCTTCCC AACAAGCAAC 
AAGGAAACAA AAGAAGAGGA GGAAGCAGAG CGCTCTGAAA GGAATACATT TGCAGAAAGA 
CTGTCTGCAG TAGAAGCAAT TGCCAATGCT ATATCTGTGG TTTCTAGCAA TGGTCCCGGA 
AATCGTGCTG GATCGTCCAG CAGTAGAAGC TTGAGATTGA GGGAAATGAT GAGGAGGTCC 
CTTCGGGCAG CAGGTTTAGG AAGACATGAG GCTGGTGCTT CTAGTGATCA CCAAGACCCA 
GTATCTCCTC CCATTGCACC TCCTAGCTGG GTTCCTGATC CACCTGCTAT GGATCCAGAT 
GGTGACATAG ATTTTATCCT GGCCCCTGCT GTAGGATCTC TTACCACTGC ATCCACTGTA 
CCTGGCCAAG GACCAAATAC TTCTACAATA CCAGGTCCTT CCTCAGAGCC ATCAGTTGTT 
GAGTCCAAGG ATCGGAAGGC AAATGCTCAC TTCATACTGA AGTTATTATG CGATAGCATC 
GTTCTGCAGC CTTACCTACG TGATCTCCTG TCTGCCAAGG ATGCAAGAGG TATGACTCCC 
TTCATGTCTG CTGTTAGTGG AAGAGCCTAT CCAGCTGCAA TTACCATACT GGAAACTGCA 
CAGAAAATCG CAAAAGCTGA AGCAAATTCA AGTGAGAAAG AGGAGGATAT TTTCAAGGGA 
ATGGTATGTC CACCTGGTAC CAATGCAGAC GACTCTCCTC TATATGTACT TTGCTGCAAT 
GATACCTGCA GTTTTACATG GACAGGAGCT GAGCATATCA ATCAGGACAT ATTTGAATGT 
CGAACATGTG GCTTGTTGGA ATCCCTGTGC TGCTGTACCG AGTGTGCAAG AGTCTGCCAC 
AAAGGCCATG ACTGCAAATT GAAAAGAACA TCACCTACTG CTTACTGCGA CTGTTGGGAG 
AAATGCAAAT GCAAAACATT AATTGCCGGG CAAAAGTCTG CACGACTTGA TCTGCTCTAT 
CGACTGCTGA CCATCACTAA TCTTGTTACA ATGCCAAATA GCAGGGGAGA GCACCTACTT 
CTTTTCTTAG TGCAGACTGT AGCCAGACAG ACAGTGGAGC ACTGCCAGTA CCGACCTCCA 
AGGATTCGAG AAGATCGCAA TCGAAAGGCA GCTAACCCTG AAGATTCCGA CATGCCAGAT 
CATGACCTAG AGCCCCCTAG GTTTGCCCAG CTTGCACTGG AACGTGTTTT ACAAGATTGG 
AACGCACTCA AATCCATGAT TATGTTTGGA TCCCAAGAAA ATAAAGACCC CCTTAGTGCT 
AGCAGTAGAA TCGGGCATCT TCTTCCAGAG GAACAAGTTT ACTTGAATCA GCAGAGTGGT 
GCCATTCGCT TGGACTGTTT TACTCATTGC CTAATTGTCA AGTGCACAGC AGATATTTTG 
CTGTTGGATA CCTTGCTGGG AACCCTTGTA AAAGAACTCC AAAATAAATA TACCCCTGGG 
CGTAGAGAAG AAGCTGTAGC TGTCACAATG CGTTTTCTGC GTTCAGTGGC TCGTGTTTTT 
GTCATTCTCA GTGTAGAAAT GGCTTCATCC AAGAAGAAAA ACAATTTCAT TCCACAGCCA 
ATAGGGAAAT GCAAGCGAGT GTTCCAAGCT TTACTTCCAT ATGCAGTGGA GGAGTTGTGT 
AATGTTGCAG AATCCCTCAT TGTCCCAGTG AGAATGGGCA TTGCACGCCC CACTGCCCCC 
TTCACATTAG CAAGTACAAG CATAGATGCC ATGCAGGGCA GCGAGGAGCT GTTCTCTGTG 
GAGCCTCTTC CTCCGAGACC TTCATCTGAT CAGTCTAACA GTTCGAATCA GTCCCAGTCA 
TCCTATATCA TTAGGAATCC TCCGCAAAGA AGAATAAGCC AGTCCCAGCC TGTGCGAGGC 
CGAGATGAAG AGCAGGATGA CATTGTATCA GCAGATGTGG AAGAGGTTGA AGTGGTAGAG 
GGGGTAGCAG GAGAAGAGGA CCATCATGAT GAGCAAGAAG AGCATGGGGA GGAGAATACA 
GAAGCAGAAG GACAACATGA TGAGCACGAT GAAGATGGTA GTGACATGGA GTTAGACCTT 
CTTGCTGCAG CTGAAACAGA AAGTGACAGT GAAAGTAACC ACAGCAATCA GGATAATGCC 
AGCGGTCGGA GGAGTGTAGT AACAGCAGCT ACCGCTGGAT CAGAAGCAGG TGCCAGCAGC 
GTTCCGGCCT TTTTCTCTGA AGATGACTCC CAATCCAATG ACTCCAGTGA TTCTGATAGC 
AGCAGCAGTC AAAGTGATGA CCTGGACCAG GAGACCTTTA TGATTGATGA GCCTCTAGAG 
AGGTCGTCCA ACAGCAATCA TGCAAATGGG GCTGCCCAGG CTCCAAGGTC AATGCAGTGG 
GCAGTACGGA ACACTCAGAG CCAAAGAGCC CCAAACACCA CACCATCTAA TACTTCTACT 
CCAGCAGCCA GTTCTGGTGC CTTGATTTAC ATTGAACCAT CCAGTCTGAG GCGCACTGGC 
AACATAAGCT CCAGTGCAGC AGCAGCAGCC GCAGCCCTTG AAGCAAGCAA TTCTAGCAGT 
TATCTAACTT CAGCAAGCAG TCTGGCTAGA GCCTACAGCA TAGTGATACG GCAGATATCT 
GACTTGATGG GTCTAATCCC CAAGTACAAC CACTTGGTCT ATTCACAGAT CCCAGCAGCC 
GTCAAACTGA ACTATCAAGA TGCCGTTAAC TTACAGAACT ATGTTGAAGA AAAACTTATT 
CCCACATGGA ACTGGATGGT GAGCATAATG GACTCCACTG AAGCCCAGCT CCGTTATGGC 
TCCGCCTTAT CATCCGCTGG AGATCCAGGT CATCCCAACC ATCCTCTTCA TGCATCTCAG 
AATTCCAACC GCAGGGAAAG GATGACTGCC CGTGAGGAAG CTAGTTTGAG GACTCTTGAG 
GGAAGAAGAA GAGCCACTCT GCTTACTGCT CGACAGGGGA TGTCGGCCCG TGGAGACTTC 
CTGAATTATG CACTTTCACT AATGCGTTCG CACAATGATG AGCACTCTGA TGTACTCCCT 
GTTTTGGATG TTTGCTCCCT GAAACACGTG GCCTATGTCT TCCAGGCTCT GATATACTGG 
ATTAAGGCAA TGAACCAACA AACAACATTG GATACTCCAC AGCTGGAAAG AAAGAGGACA 
AGAGAGCTTT TGGAGCTTGG CCTTGATAAT GAAGATTCAG AGCATGAAAA TGATGATGAC 
ACAAATCAAA GCCTAATATT GAATGACAAA GACGACGATA CACTACCCTC TGAGACCGGT 
CCAAACCATC CGTTTTTCCG TCGTTCCGAT TCAATGACGT TCCTTGGTTG TATTCCACCA 
AACCCCTTTG AAGTTCCTCT GGCAGAAGCT ATCCCACTTG CAGACCAGCC CCATCTTTTG 
CAGCCAAATG CAAGGAAAGA AGACTTATTT GGTCGACCGA GTCAAGGTCT CTACTCATCT 
ACTGCTAACA CTGGAAAATG CCTATTAGAA GTGACAGTTG ACAGAAACTG CCTGGAGGTT 
TTACCAACCA AAATGTCATA TGCTGCTAAT CTAAAAAATG TAATGAACAT GCAGAGCCGT 
CAGAAAGAAG CAGAGGAACA GAATGTGCCG GCTGAGGAAA ATGACACATC AAAGGCTGGA 
CCCTCAGCTC ATGATCTTGC AGCTCAGCTG AAAAGTAGCT TGTTGGCAGA AATAGGGTTA 
ACAGAGAGTG AAGGTCCCCC GCTTACATCA TTCAGGCCAC AGTGTAGCTT TATGGGAATG 
GTCATTTCCC ATGATATGCT GTTAGGACGT TGGCGACTAT CTCTTGAATT GTTTGGAAGG 
GTGTTTATGG AAGATGTCGG AGCAGAGCCT GGATCGATCT TAACTGAACT AGGAGGGTTT 
GAAGTAAAGG AATCCAAGTT CCGCAGGGAG ATGGAAAAAC TTCGAAATCA GCAGTCCAGA 
GATCTTACAC TAGAGGTGGA TCGAGATAGA GATCTTCTAA TACAGCAAAC GATGAGGCAG 
CTGAATAATC ATTTTGGGCG CAGATGCGCC ACAACTCCTA TGGCAGTGCA TAGAGTAAAG 
GTCACCTTTA AAGATGAGCC AGGAGAAGGC AGTGGAGTAG CCCGAAGTTT TTATACTGCA 
GTAGCACAAG CATTTTTGTC CAATGAAAAG TTGCCAAGCT TGGACTGTAT CCAAAATGCC 
AACAAAGGTA CTCATACAAG TTTGATGCAA AGACTCAGAA ATCGTGGGGA ACGAGACCGG 
GAAAGGGAGC GGGACATGAG AAGAAGCAGT GGTTTGCGAA CAGGTTCTCG GCGGGATAGA 
GACAGAGATT TCAGACGGCA ACTTTCCATT GATACTAGGC CTTTCAGGCC AGCTTCTGAG 
GGCAATCCTA GTGATGATCC TGATCCTCTT CCTGCTCACC GACAGGCTCT CGGAGAAAGA 
CTATACCCAC GTGTACAAGC CATGCAGCCG GCATTTGCAA GTAAAATTAC AGGCATGCTG 
CTTGAGCTAT CTCCTGCACA ACTGCTGCTG CTGTTGGCAA GTGAAGACTC TCTAAGAGCC 
AGAGTGGATG AAGCTATGGA ACTTATTATT GCACATGGAA GGGAGAATGG TGCTGACAGT 
ATCCTCGATT TAGGTTTATT AGAAGCAGCT GATAAGGTAC AGGAAAACCG TAAGCGCCAT 
GGCTCTAGTC GAAGCGTGGT TGATATGGAG CTTACTGACA CAGACGATGG GGACGATAAT 
GCTCCTTTAT TTTACCAACC TGGTAAACGA GGTTTCTATA GTCCAAGGCC TGGAAAAAAC 
ACAGAGGCAC GTTTAAACTG TTTCCGCAAC ATCGGCAGAA TTCTTGGTCT GTGCCTACTA 
CAGAATGAAT TATGTCCTAT CACTTTAAGT AGGCAGGTGA TTAAAGTGCT TCTGGGCAGA 
AAGGTGAACT GGCATGATTT TGCGTTCTTT GATCACGTGA TGTACGAGAG CTTACGGCAA 
CTTATACTCG CCTCCCAAAG CCCGGATGCT GACGCAGTGT TTGCCTCAAT GGACCTAGCG 
TTTGCAATAG ACCTCTGCAA AGAAGAAGGT GGTGGACAAG TGGAACTTAT TCCTAATGGC 
GTAAATATCC CTGTAACCCC TCAAAATGTG TATGAATATG TAAGAAAATA TGCTGAACAC 
AGAATGCTCA CTGTAGCAGA GCAACCGCTT AATGCCATGA GGAAAGGACT ATTGGATGTT 
CTCCCAAAGA ATGCATTAGA AGACTTAACT GCAGAAGACT TTAGGCTCTT AGTGAATGGC 
TGTGGGGAAG TTAATGTTCA GATGCTCATT AGCTTTACCT CCTTCAACGA TGAGTCAGGA 
GAAAACGCAG AAAAGCTGTT GCAGTTCAAA CGCTGGTTCT GGTCAATCGT AGAGAAGATG 
AGTATGACAG AGCGGCAAGA TTTAGTTTAT TTTTGGACCT CAAGCCCATC ATTGCCTGCG 
AGTGAAGAGG GATTCCAGCC CATGCCATCC ATTACCATCC GGCCCCCCGA TGATCAGCAC 
CTTCCCACGG CGAACACCTG CATCTCTCGC CTCTACGTCC CCCTTTATTC TTCCAAACAG 
ATCCTGAAAC AGAAGTTGTT ACTTGCTATT AAGACCAAGA ACTTTGGATT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018079386.1
CDS	438..8834
Translation	MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLSSHPPLNVLEQTTIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELSKPENNDGSKLSSGSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGEDLMSLLDADIHSAHPSVIIDADAMFSEDISYFGYPSFRRSSLSRIGSSRVLLLPLERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPTLDKKNTPIMSPVSLGEDLQWWPEKDGTKFISIGALYSELVAVSSKGELYQWKWIEVEPYRNTQNPSLRHPRTSFLGLTNEKIVLLSANSIRATVATESNKVATWVDETLSSVASKLEHTAQIFPELQGDKIVSLHCCALYTCAQLENNLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNLEKTNKTIEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEERVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSNNSNNSNNSNNPCTPGSDPDPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGTWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLTNLPANSTIKKKAAIIIMAVEKQTLMQHILRGDYEACRQYLMNLEQGVVLDPNTQMLQTFLSHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTASTVPGQGPNTSTIPGPSSEPSVVESKDRKANAHFILKLLCDSIVLQPYLRDLLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEANSSEKEEDIFKGMVCPPGTNADDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTITNLVTMPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKAANPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGAIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAVAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSNSSNQSQSSYIIRNPPQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENTEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDLDQETFMIDEPLERSSNSNHANGAAQAPRSMQWAVRNTQSQRAPNTTPSNTSTPAASSGALIYIEPSSLRRTGNISSSAAAAAAALEASNSSSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLNYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALSSAGDPGHPNHPLHASQNSNRRERMTAREEASLRTLEGRRRATLLTARQGMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGLDNEDSEHENDDDTNQSLILNDKDDDTLPSETGPNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSTANTGKCLLEVTVDRNCLEVLPTKMSYAANLKNVMNMQSRQKEAEEQNVPAEENDTSKAGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLTLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAVAQAFLSNEKLPSLDCIQNANKGTHTSLMQRLRNRGERDRERERDMRRSSGLRTGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPDPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLLEAADKVQENRKRHGSSRSVVDMELTDTDDGDDNAPLFYQPGKRGFYSPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLSRQVIKVLLGRKVNWHDFAFFDHVMYESLRQLILASQSPDADAVFASMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLTVAEQPLNAMRKGLLDVLPKNALEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV

Target ORF information:

RefSeq Version	XM_018223897.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018223897.1

ORF Insert Sequence:

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGCAGG TGGAGTTCTG GTGTTGGTGG AAGTGGTGGG 
GGATCCTCTG GGCGGTCATC AGCTGGTGCT AGAGACTCTC GGAGGCAAAC AAGAGTTATA 
CGCACAGGAA GGGACAGGGG CTCTGGTCTC CTGGGCAGTC AACCACAGCC TGTGATTCCA 
GCTTCTGTTA TTCCTGAGGA GCTCATCTCA CAGGCACAAG TTGTTCTTCA AGGAAAATCA 
CGAAGTGTTA TCATTCGAGA GCTGCAGAGA ACAAATCTTG ATGTGAACCT TGCAGTAAAC 
AATCTCTTGA GTCGAGATGA TGAAGATGGG GATGATGGTG ATGACACTGC TAGTGAATCT 
TACTTGCCTG GAGAAGATCT TATGTCACTG CTGGATGCTG ACATTCATTC TGCTCACCCA 
AGTGTCATAA TTGATGCTGA TGCTATGTTT TCAGAAGATA TTAGCTACTT TGGTTATCCA 
TCTTTTCGGC GCTCTTCACT TTCCAGGATA GGCTCATCCA GAGTTCTCCT TCTTCCCTTA 
GAGAGAGACT CTGAGCTGTT GCGTGAACGT GAGTCAGTGT TGCGTTTGCG TGAGCGGAGG 
TGGCTTGATG GGGCCTCGTT TGATAATGAA AGAGGCTCCA CAAGTAAAGA AGGGGAACCT 
ACACTCGATA AGAAGAACAC TCCCATTATG AGCCCAGTGT CACTGGGAGA AGACCTGCAA 
TGGTGGCCTG AAAAGGATGG GACAAAGTTT ATTTCTATTG GAGCACTTTA TTCCGAGCTG 
GTTGCTGTAA GCAGTAAGGG AGAACTTTAT CAGTGGAAGT GGATCGAGGT GGAGCCTTAT 
AGGAATACAC AGAATCCATC ATTACGTCAT CCAAGAACAT CCTTTTTAGG CCTAACAAAT 
GAGAAAATAG TCCTGTTGTC AGCAAACAGT ATCCGAGCCA CAGTCGCTAC AGAAAGCAAC 
AAGGTTGCCA CTTGGGTCGA TGAAACATTA AGCTCGGTAG CCTCAAAGTT AGAACACACA 
GCTCAGATTT TCCCTGAGCT ACAAGGAGAC AAGATTGTCT CGTTGCACTG TTGTGCACTT 
TATACTTGTG CGCAATTAGA AAATAACCTT TATTGGTGGG GTGTGGTTCC TTTCAGTCAG 
AGGAAGAAAA TGCTTGAAAA AGCTCGTGCA AAGAATAAAA AACCCAAATC TAGTGCCGGA 
ATTTCATCCA TGCCAAATAT CACAGTTGGC ACCCAGGTAT GCTTAAGAAA TAACCCTCTC 
TACCATGCGG GAGCTGTTGC TTTCTCGATC AGTGCTGGCA TTCCCAAAGT AGGAGTATTA 
ATGGAATCTG TGTGGAATAT GAATGATAGC TGCAGATTTC AGCTCCGTTC TCCAGAGAGT 
TTAAAAAACT TGGAAAAAAC AAATAAAACA ATTGAAGCCA AACCTGAGAG CAAACAGGAA 
CCTGTAAAAA CAGAGATGGG CCCGCCCCCA TCTCCCGCAT CTACCTGTAG TGATGCCTCT 
TCAATTGCTA GCAGTGCCTC CATGCCATAC AAACGAAGAC GATCAACTCC TGCTCCTAAG 
GAAGAAGAGA GAGTGAATGA AGAACAGTGG TCTCTCAGAG AAGTTGTTTT TGTAGAAGAT 
GTTAAAAATG TTCCTGTTGG GAAGGTACTA AAAGTTGATG GGGCCTATGT TGCAGTCAAA 
TTTCCTGGCA CTTCTAATAA TTCTAATAAT TCTAATAATT CTAACAATCC GTGCACCCCT 
GGCTCTGATC CAGACCCATC CTCTCTCCTC CAGGATTGTA GATTACTACG GATTGATGAA 
CTACAGGTAG TGAAAACTGG GGGGACACCC AAAGTCCCAG ACTGTTTCCA AAGGACACCT 
AAAAAGCTGT GCATTCCTGA AAAAACTGAG ATCCTGGCAG TCAATGTAGA TTCCAAAGGG 
GTTCACGCGG TACTGAAAAC AGGGACATGG GTACGGTACT GTATCTTTGA TCTTGCAACT 
GGAAAAGCAG AACAGGAAAA CAACTTCCCA ACAAGTAGCA TTGCATTTCT TGGCCAGAAT 
GAGAGAAATG TTGCTATTTT TACAGCTGGG CAGGAATCTC CCATCATTCT TAGAGATGGC 
AATGGTACCA TTTATCCCAT GGCAAAAGAT TGCATGGGTG GGATAAGAGA TCCAGACTGG 
CTTGACCTTC CTCCAATAAG CAGTCTTGGA ATGGGAGTTC ATTCTTTAAC CAATCTCCCT 
GCAAATTCAA CAATTAAGAA GAAAGCAGCC ATTATCATAA TGGCTGTTGA GAAGCAAACA 
CTGATGCAAC ACATTTTGCG CGGTGATTAT GAGGCCTGCA GGCAGTATTT GATGAACCTA 
GAGCAAGGTG TAGTTTTGGA TCCAAACACT CAAATGCTGC AGACTTTTCT GAGCCACAGA 
TGTGATGGCA ATAGGAACAT ATTGCATGCC TGCGTGTCCG TTTGCTTCCC AACAAGCAAC 
AAGGAAACAA AAGAAGAGGA GGAAGCAGAG CGCTCTGAAA GGAATACATT TGCAGAAAGA 
CTGTCTGCAG TAGAAGCAAT TGCCAATGCT ATATCTGTGG TTTCTAGCAA TGGTCCCGGA 
AATCGTGCTG GATCGTCCAG CAGTAGAAGC TTGAGATTGA GGGAAATGAT GAGGAGGTCC 
CTTCGGGCAG CAGGTTTAGG AAGACATGAG GCTGGTGCTT CTAGTGATCA CCAAGACCCA 
GTATCTCCTC CCATTGCACC TCCTAGCTGG GTTCCTGATC CACCTGCTAT GGATCCAGAT 
GGTGACATAG ATTTTATCCT GGCCCCTGCT GTAGGATCTC TTACCACTGC ATCCACTGTA 
CCTGGCCAAG GACCAAATAC TTCTACAATA CCAGGTCCTT CCTCAGAGCC ATCAGTTGTT 
GAGTCCAAGG ATCGGAAGGC AAATGCTCAC TTCATACTGA AGTTATTATG CGATAGCATC 
GTTCTGCAGC CTTACCTACG TGATCTCCTG TCTGCCAAGG ATGCAAGAGG TATGACTCCC 
TTCATGTCTG CTGTTAGTGG AAGAGCCTAT CCAGCTGCAA TTACCATACT GGAAACTGCA 
CAGAAAATCG CAAAAGCTGA AGCAAATTCA AGTGAGAAAG AGGAGGATAT TTTCAAGGGA 
ATGGTATGTC CACCTGGTAC CAATGCAGAC GACTCTCCTC TATATGTACT TTGCTGCAAT 
GATACCTGCA GTTTTACATG GACAGGAGCT GAGCATATCA ATCAGGACAT ATTTGAATGT 
CGAACATGTG GCTTGTTGGA ATCCCTGTGC TGCTGTACCG AGTGTGCAAG AGTCTGCCAC 
AAAGGCCATG ACTGCAAATT GAAAAGAACA TCACCTACTG CTTACTGCGA CTGTTGGGAG 
AAATGCAAAT GCAAAACATT AATTGCCGGG CAAAAGTCTG CACGACTTGA TCTGCTCTAT 
CGACTGCTGA CCATCACTAA TCTTGTTACA ATGCCAAATA GCAGGGGAGA GCACCTACTT 
CTTTTCTTAG TGCAGACTGT AGCCAGACAG ACAGTGGAGC ACTGCCAGTA CCGACCTCCA 
AGGATTCGAG AAGATCGCAA TCGAAAGGCA GCTAACCCTG AAGATTCCGA CATGCCAGAT 
CATGACCTAG AGCCCCCTAG GTTTGCCCAG CTTGCACTGG AACGTGTTTT ACAAGATTGG 
AACGCACTCA AATCCATGAT TATGTTTGGA TCCCAAGAAA ATAAAGACCC CCTTAGTGCT 
AGCAGTAGAA TCGGGCATCT TCTTCCAGAG GAACAAGTTT ACTTGAATCA GCAGAGTGGT 
GCCATTCGCT TGGACTGTTT TACTCATTGC CTAATTGTCA AGTGCACAGC AGATATTTTG 
CTGTTGGATA CCTTGCTGGG AACCCTTGTA AAAGAACTCC AAAATAAATA TACCCCTGGG 
CGTAGAGAAG AAGCTGTAGC TGTCACAATG CGTTTTCTGC GTTCAGTGGC TCGTGTTTTT 
GTCATTCTCA GTGTAGAAAT GGCTTCATCC AAGAAGAAAA ACAATTTCAT TCCACAGCCA 
ATAGGGAAAT GCAAGCGAGT GTTCCAAGCT TTACTTCCAT ATGCAGTGGA GGAGTTGTGT 
AATGTTGCAG AATCCCTCAT TGTCCCAGTG AGAATGGGCA TTGCACGCCC CACTGCCCCC 
TTCACATTAG CAAGTACAAG CATAGATGCC ATGCAGGGCA GCGAGGAGCT GTTCTCTGTG 
GAGCCTCTTC CTCCGAGACC TTCATCTGAT CAGTCTAACA GTTCGAATCA GTCCCAGTCA 
TCCTATATCA TTAGGAATCC TCCGCAAAGA AGAATAAGCC AGTCCCAGCC TGTGCGAGGC 
CGAGATGAAG AGCAGGATGA CATTGTATCA GCAGATGTGG AAGAGGTTGA AGTGGTAGAG 
GGGGTAGCAG GAGAAGAGGA CCATCATGAT GAGCAAGAAG AGCATGGGGA GGAGAATACA 
GAAGCAGAAG GACAACATGA TGAGCACGAT GAAGATGGTA GTGACATGGA GTTAGACCTT 
CTTGCTGCAG CTGAAACAGA AAGTGACAGT GAAAGTAACC ACAGCAATCA GGATAATGCC 
AGCGGTCGGA GGAGTGTAGT AACAGCAGCT ACCGCTGGAT CAGAAGCAGG TGCCAGCAGC 
GTTCCGGCCT TTTTCTCTGA AGATGACTCC CAATCCAATG ACTCCAGTGA TTCTGATAGC 
AGCAGCAGTC AAAGTGATGA CCTGGACCAG GAGACCTTTA TGATTGATGA GCCTCTAGAG 
AGGTCGTCCA ACAGCAATCA TGCAAATGGG GCTGCCCAGG CTCCAAGGTC AATGCAGTGG 
GCAGTACGGA ACACTCAGAG CCAAAGAGCC CCAAACACCA CACCATCTAA TACTTCTACT 
CCAGCAGCCA GTTCTGGTGC CTTGATTTAC ATTGAACCAT CCAGTCTGAG GCGCACTGGC 
AACATAAGCT CCAGTGCAGC AGCAGCAGCC GCAGCCCTTG AAGCAAGCAA TTCTAGCAGT 
TATCTAACTT CAGCAAGCAG TCTGGCTAGA GCCTACAGCA TAGTGATACG GCAGATATCT 
GACTTGATGG GTCTAATCCC CAAGTACAAC CACTTGGTCT ATTCACAGAT CCCAGCAGCC 
GTCAAACTGA ACTATCAAGA TGCCGTTAAC TTACAGAACT ATGTTGAAGA AAAACTTATT 
CCCACATGGA ACTGGATGGT GAGCATAATG GACTCCACTG AAGCCCAGCT CCGTTATGGC 
TCCGCCTTAT CATCCGCTGG AGATCCAGGT CATCCCAACC ATCCTCTTCA TGCATCTCAG 
AATTCCAACC GCAGGGAAAG GATGACTGCC CGTGAGGAAG CTAGTTTGAG GACTCTTGAG 
GGAAGAAGAA GAGCCACTCT GCTTACTGCT CGACAGGGGA TGTCGGCCCG TGGAGACTTC 
CTGAATTATG CACTTTCACT AATGCGTTCG CACAATGATG AGCACTCTGA TGTACTCCCT 
GTTTTGGATG TTTGCTCCCT GAAACACGTG GCCTATGTCT TCCAGGCTCT GATATACTGG 
ATTAAGGCAA TGAACCAACA AACAACATTG GATACTCCAC AGCTGGAAAG AAAGAGGACA 
AGAGAGCTTT TGGAGCTTGG CCTTGATAAT GAAGATTCAG AGCATGAAAA TGATGATGAC 
ACAAATCAAA GCCTAATATT GAATGACAAA GACGACGATA CACTACCCTC TGAGACCGGT 
CCAAACCATC CGTTTTTCCG TCGTTCCGAT TCAATGACGT TCCTTGGTTG TATTCCACCA 
AACCCCTTTG AAGTTCCTCT GGCAGAAGCT ATCCCACTTG CAGACCAGCC CCATCTTTTG 
CAGCCAAATG CAAGGAAAGA AGACTTATTT GGTCGACCGA GTCAAGGTCT CTACTCATCT 
ACTGCTAACA CTGGAAAATG CCTATTAGAA GTGACAGTTG ACAGAAACTG CCTGGAGGTT 
TTACCAACCA AAATGTCATA TGCTGCTAAT CTAAAAAATG TAATGAACAT GCAGAGCCGT 
CAGAAAGAAG CAGAGGAACA GAATGTGCCG GCTGAGGAAA ATGACACATC AAAGGCTGGA 
CCCTCAGCTC ATGATCTTGC AGCTCAGCTG AAAAGTAGCT TGTTGGCAGA AATAGGGTTA 
ACAGAGAGTG AAGGTCCCCC GCTTACATCA TTCAGGCCAC AGTGTAGCTT TATGGGAATG 
GTCATTTCCC ATGATATGCT GTTAGGACGT TGGCGACTAT CTCTTGAATT GTTTGGAAGG 
GTGTTTATGG AAGATGTCGG AGCAGAGCCT GGATCGATCT TAACTGAACT AGGAGGGTTT 
GAAGTAAAGG AATCCAAGTT CCGCAGGGAG ATGGAAAAAC TTCGAAATCA GCAGTCCAGA 
GATCTTACAC TAGAGGTGGA TCGAGATAGA GATCTTCTAA TACAGCAAAC GATGAGGCAG 
CTGAATAATC ATTTTGGGCG CAGATGCGCC ACAACTCCTA TGGCAGTGCA TAGAGTAAAG 
GTCACCTTTA AAGATGAGCC AGGAGAAGGC AGTGGAGTAG CCCGAAGTTT TTATACTGCA 
GTAGCACAAG CATTTTTGTC CAATGAAAAG TTGCCAAGCT TGGACTGTAT CCAAAATGCC 
AACAAAGGTA CTCATACAAG TTTGATGCAA AGACTCAGAA ATCGTGGGGA ACGAGACCGG 
GAAAGGGAGC GGGACATGAG AAGAAGCAGT GGTTTGCGAA CAGGTTCTCG GCGGGATAGA 
GACAGAGATT TCAGACGGCA ACTTTCCATT GATACTAGGC CTTTCAGGCC AGCTTCTGAG 
GGCAATCCTA GTGATGATCC TGATCCTCTT CCTGCTCACC GACAGGCTCT CGGAGAAAGA 
CTATACCCAC GTGTACAAGC CATGCAGCCG GCATTTGCAA GTAAAATTAC AGGCATGCTG 
CTTGAGCTAT CTCCTGCACA ACTGCTGCTG CTGTTGGCAA GTGAAGACTC TCTAAGAGCC 
AGAGTGGATG AAGCTATGGA ACTTATTATT GCACATGGAA GGGAGAATGG TGCTGACAGT 
ATCCTCGATT TAGGTTTATT AGAAGCAGCT GATAAGGTAC AGGAAAACCG TAAGCGCCAT 
GGCTCTAGTC GAAGCGTGGT TGATATGGAG CTTACTGACA CAGACGATGG GGACGATAAT 
GCTCCTTTAT TTTACCAACC TGGTAAACGA GGTTTCTATA GTCCAAGGCC TGGAAAAAAC 
ACAGAGGCAC GTTTAAACTG TTTCCGCAAC ATCGGCAGAA TTCTTGGTCT GTGCCTACTA 
CAGAATGAAT TATGTCCTAT CACTTTAAGT AGGCAGGTGA TTAAAGTGCT TCTGGGCAGA 
AAGGTGAACT GGCATGATTT TGCGTTCTTT GATCACGTGA TGTACGAGAG CTTACGGCAA 
CTTATACTCG CCTCCCAAAG CCCGGATGCT GACGCAGTGT TTGCCTCAAT GGACCTAGCG 
TTTGCAATAG ACCTCTGCAA AGAAGAAGGT GGTGGACAAG TGGAACTTAT TCCTAATGGC 
GTAAATATCC CTGTAACCCC TCAAAATGTG TATGAATATG TAAGAAAATA TGCTGAACAC 
AGAATGCTCA CTGTAGCAGA GCAACCGCTT AATGCCATGA GGAAAGGACT ATTGGATGTT 
CTCCCAAAGA ATGCATTAGA AGACTTAACT GCAGAAGACT TTAGGCTCTT AGTGAATGGC 
TGTGGGGAAG TTAATGTTCA GATGCTCATT AGCTTTACCT CCTTCAACGA TGAGTCAGGA 
GAAAACGCAG AAAAGCTGTT GCAGTTCAAA CGCTGGTTCT GGTCAATCGT AGAGAAGATG 
AGTATGACAG AGCGGCAAGA TTTAGTTTAT TTTTGGACCT CAAGCCCATC ATTGCCTGCG 
AGTGAAGAGG GATTCCAGCC CATGCCATCC ATTACCATCC GGCCCCCCGA TGATCAGCAC 
CTTCCCACGG CGAACACCTG CATCTCTCGC CTCTACGTCC CCCTTTATTC TTCCAAACAG 
ATCCTGAAAC AGAAGTTGTT ACTTGCTATT AAGACCAAGA ACTTTGGATT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108695408 ( XM_018223898.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018079387.1 Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X4, mRNA.

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CloneID	OAf37366
Clone ID Related Accession (Same CDS sequence)	XM_018223898.1
Accession Version	XM_018223898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase UBR5-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAAGA TCTTATGTCA CTGCTGGATG CTGACATTCA TTCTGCTCAC 
CCAAGTGTCA TAATTGATGC TGATGCTATG TTTTCAGAAG ATATTAGCTA CTTTGGTTAT 
CCATCTTTTC GGCGCTCTTC ACTTTCCAGG ATAGGCTCAT CCAGAGTTCT CCTTCTTCCC 
TTAGAGAGAG ACTCTGAGCT GTTGCGTGAA CGTGAGTCAG TGTTGCGTTT GCGTGAGCGG 
AGGTGGCTTG ATGGGGCCTC GTTTGATAAT GAAAGAGGCT CCACAAGTAA AGAAGGGGAA 
CCTACACTCG ATAAGAAGAA CACTCCCATT ATGAGCCCAG TGTCACTGGG AGAAGACCTG 
CAATGGTGGC CTGAAAAGGA TGGGACAAAG TTTATTTCTA TTGGAGCACT TTATTCCGAG 
CTGGTTGCTG TAAGCAGTAA GGGAGAACTT TATCAGTGGA AGTGGATCGA GGTGGAGCCT 
TATAGGAATA CACAGAATCC ATCATTACGT CATCCAAGAA CATCCTTTTT AGGCCTAACA 
AATGAGAAAA TAGTCCTGTT GTCAGCAAAC AGTATCCGAG CCACAGTCGC TACAGAAAGC 
AACAAGGTTG CCACTTGGGT CGATGAAACA TTAAGCTCGG TAGCCTCAAA GTTAGAACAC 
ACAGCTCAGA TTTTCCCTGA GCTACAAGGA GACAAGATTG TCTCGTTGCA CTGTTGTGCA 
CTTTATACTT GTGCGCAATT AGAAAATAAC CTTTATTGGT GGGGTGTGGT TCCTTTCAGT 
CAGAGGAAGA AAATGCTTGA AAAAGCTCGT GCAAAGAATA AAAAACCCAA ATCTAGTGCC 
GGAATTTCAT CCATGCCAAA TATCACAGTT GGCACCCAGG TATGCTTAAG AAATAACCCT 
CTCTACCATG CGGGAGCTGT TGCTTTCTCG ATCAGTGCTG GCATTCCCAA AGTAGGAGTA 
TTAATGGAAT CTGTGTGGAA TATGAATGAT AGCTGCAGAT TTCAGCTCCG TTCTCCAGAG 
AGTTTAAAAA ACTTGGAAAA AACAAATAAA ACAATTGAAG CCAAACCTGA GAGCAAACAG 
GAACCTGTAA AAACAGAGAT GGGCCCGCCC CCATCTCCCG CATCTACCTG TAGTGATGCC 
TCTTCAATTG CTAGCAGTGC CTCCATGCCA TACAAACGAA GACGATCAAC TCCTGCTCCT 
AAGGAAGAAG AGAGAGTGAA TGAAGAACAG TGGTCTCTCA GAGAAGTTGT TTTTGTAGAA 
GATGTTAAAA ATGTTCCTGT TGGGAAGGTA CTAAAAGTTG ATGGGGCCTA TGTTGCAGTC 
AAATTTCCTG GCACTTCTAA TAATTCTAAT AATTCTAATA ATTCTAACAA TCCGTGCACC 
CCTGGCTCTG ATCCAGACCC ATCCTCTCTC CTCCAGGATT GTAGATTACT ACGGATTGAT 
GAACTACAGG TAGTGAAAAC TGGGGGGACA CCCAAAGTCC CAGACTGTTT CCAAAGGACA 
CCTAAAAAGC TGTGCATTCC TGAAAAAACT GAGATCCTGG CAGTCAATGT AGATTCCAAA 
GGGGTTCACG CGGTACTGAA AACAGGGACA TGGGTACGGT ACTGTATCTT TGATCTTGCA 
ACTGGAAAAG CAGAACAGGA AAACAACTTC CCAACAAGTA GCATTGCATT TCTTGGCCAG 
AATGAGAGAA ATGTTGCTAT TTTTACAGCT GGGCAGGAAT CTCCCATCAT TCTTAGAGAT 
GGCAATGGTA CCATTTATCC CATGGCAAAA GATTGCATGG GTGGGATAAG AGATCCAGAC 
TGGCTTGACC TTCCTCCAAT AAGCAGTCTT GGAATGGGAG TTCATTCTTT AACCAATCTC 
CCTGCAAATT CAACAATTAA GAAGAAAGCA GCCATTATCA TAATGGCTGT TGAGAAGCAA 
ACACTGATGC AACACATTTT GCGCGGTGAT TATGAGGCCT GCAGGCAGTA TTTGATGAAC 
CTAGAGCAAG GTGTAGTTTT GGATCCAAAC ACTCAAATGC TGCAGACTTT TCTGAGCCAC 
AGATGTGATG GCAATAGGAA CATATTGCAT GCCTGCGTGT CCGTTTGCTT CCCAACAAGC 
AACAAGGAAA CAAAAGAAGA GGAGGAAGCA GAGCGCTCTG AAAGGAATAC ATTTGCAGAA 
AGACTGTCTG CAGTAGAAGC AATTGCCAAT GCTATATCTG TGGTTTCTAG CAATGGTCCC 
GGAAATCGTG CTGGATCGTC CAGCAGTAGA AGCTTGAGAT TGAGGGAAAT GATGAGGAGG 
TCCCTTCGGG CAGCAGGTTT AGGAAGACAT GAGGCTGGTG CTTCTAGTGA TCACCAAGAC 
CCAGTATCTC CTCCCATTGC ACCTCCTAGC TGGGTTCCTG ATCCACCTGC TATGGATCCA 
GATGGTGACA TAGATTTTAT CCTGGCCCCT GCTGTAGGAT CTCTTACCAC TGCATCCACT 
GTACCTGGCC AAGGACCAAA TACTTCTACA ATACCAGGTC CTTCCTCAGA GCCATCAGTT 
GTTGAGTCCA AGGATCGGAA GGCAAATGCT CACTTCATAC TGAAGTTATT ATGCGATAGC 
ATCGTTCTGC AGCCTTACCT ACGTGATCTC CTGTCTGCCA AGGATGCAAG AGGTATGACT 
CCCTTCATGT CTGCTGTTAG TGGAAGAGCC TATCCAGCTG CAATTACCAT ACTGGAAACT 
GCACAGAAAA TCGCAAAAGC TGAAGCAAAT TCAAGTGAGA AAGAGGAGGA TATTTTCAAG 
GGAATGGTAT GTCCACCTGG TACCAATGCA GACGACTCTC CTCTATATGT ACTTTGCTGC 
AATGATACCT GCAGTTTTAC ATGGACAGGA GCTGAGCATA TCAATCAGGA CATATTTGAA 
TGTCGAACAT GTGGCTTGTT GGAATCCCTG TGCTGCTGTA CCGAGTGTGC AAGAGTCTGC 
CACAAAGGCC ATGACTGCAA ATTGAAAAGA ACATCACCTA CTGCTTACTG CGACTGTTGG 
GAGAAATGCA AATGCAAAAC ATTAATTGCC GGGCAAAAGT CTGCACGACT TGATCTGCTC 
TATCGACTGC TGACCATCAC TAATCTTGTT ACAATGCCAA ATAGCAGGGG AGAGCACCTA 
CTTCTTTTCT TAGTGCAGAC TGTAGCCAGA CAGACAGTGG AGCACTGCCA GTACCGACCT 
CCAAGGATTC GAGAAGATCG CAATCGAAAG GCAGCTAACC CTGAAGATTC CGACATGCCA 
GATCATGACC TAGAGCCCCC TAGGTTTGCC CAGCTTGCAC TGGAACGTGT TTTACAAGAT 
TGGAACGCAC TCAAATCCAT GATTATGTTT GGATCCCAAG AAAATAAAGA CCCTGCTAGC 
AGTAGAATCG GGCATCTTCT TCCAGAGGAA CAAGTTTACT TGAATCAGCA GAGTGGTGCC 
ATTCGCTTGG ACTGTTTTAC TCATTGCCTA ATTGTCAAGT GCACAGCAGA TATTTTGCTG 
TTGGATACCT TGCTGGGAAC CCTTGTAAAA GAACTCCAAA ATAAATATAC CCCTGGGCGT 
AGAGAAGAAG CTGTAGCTGT CACAATGCGT TTTCTGCGTT CAGTGGCTCG TGTTTTTGTC 
ATTCTCAGTG TAGAAATGGC TTCATCCAAG AAGAAAAACA ATTTCATTCC ACAGCCAATA 
GGGAAATGCA AGCGAGTGTT CCAAGCTTTA CTTCCATATG CAGTGGAGGA GTTGTGTAAT 
GTTGCAGAAT CCCTCATTGT CCCAGTGAGA ATGGGCATTG CACGCCCCAC TGCCCCCTTC 
ACATTAGCAA GTACAAGCAT AGATGCCATG CAGGGCAGCG AGGAGCTGTT CTCTGTGGAG 
CCTCTTCCTC CGAGACCTTC ATCTGATCAG TCTAACAGTT CGAATCAGTC CCAGTCATCC 
TATATCATTA GGAATCCTCC GCAAAGAAGA ATAAGCCAGT CCCAGCCTGT GCGAGGCCGA 
GATGAAGAGC AGGATGACAT TGTATCAGCA GATGTGGAAG AGGTTGAAGT GGTAGAGGGG 
GTAGCAGGAG AAGAGGACCA TCATGATGAG CAAGAAGAGC ATGGGGAGGA GAATACAGAA 
GCAGAAGGAC AACATGATGA GCACGATGAA GATGGTAGTG ACATGGAGTT AGACCTTCTT 
GCTGCAGCTG AAACAGAAAG TGACAGTGAA AGTAACCACA GCAATCAGGA TAATGCCAGC 
GGTCGGAGGA GTGTAGTAAC AGCAGCTACC GCTGGATCAG AAGCAGGTGC CAGCAGCGTT 
CCGGCCTTTT TCTCTGAAGA TGACTCCCAA TCCAATGACT CCAGTGATTC TGATAGCAGC 
AGCAGTCAAA GTGATGACCT GGACCAGGAG ACCTTTATGA TTGATGAGCC TCTAGAGAGG 
TCGTCCAACA GCAATCATGC AAATGGGGCT GCCCAGGCTC CAAGGTCAAT GCAGTGGGCA 
GTACGGAACA CTCAGAGCCA AAGAGCCCCA AACACCACAC CATCTAATAC TTCTACTCCA 
GCAGCCAGTT CTGGTGCCTT GATTTACATT GAACCATCCA GTCTGAGGCG CACTGGCAAC 
ATAAGCTCCA GTGCAGCAGC AGCAGCCGCA GCCCTTGAAG CAAGCAATTC TAGCAGTTAT 
CTAACTTCAG CAAGCAGTCT GGCTAGAGCC TACAGCATAG TGATACGGCA GATATCTGAC 
TTGATGGGTC TAATCCCCAA GTACAACCAC TTGGTCTATT CACAGATCCC AGCAGCCGTC 
AAACTGAACT ATCAAGATGC CGTTAACTTA CAGAACTATG TTGAAGAAAA ACTTATTCCC 
ACATGGAACT GGATGGTGAG CATAATGGAC TCCACTGAAG CCCAGCTCCG TTATGGCTCC 
GCCTTATCAT CCGCTGGAGA TCCAGGTCAT CCCAACCATC CTCTTCATGC ATCTCAGAAT 
TCCAACCGCA GGGAAAGGAT GACTGCCCGT GAGGAAGCTA GTTTGAGGAC TCTTGAGGGA 
AGAAGAAGAG CCACTCTGCT TACTGCTCGA CAGGGGATGT CGGCCCGTGG AGACTTCCTG 
AATTATGCAC TTTCACTAAT GCGTTCGCAC AATGATGAGC ACTCTGATGT ACTCCCTGTT 
TTGGATGTTT GCTCCCTGAA ACACGTGGCC TATGTCTTCC AGGCTCTGAT ATACTGGATT 
AAGGCAATGA ACCAACAAAC AACATTGGAT ACTCCACAGC TGGAAAGAAA GAGGACAAGA 
GAGCTTTTGG AGCTTGGCCT TGATAATGAA GATTCAGAGC ATGAAAATGA TGATGACACA 
AATCAAAGCC TAATATTGAA TGACAAAGAC GACGATACAC TACCCTCTGA GACCGGTCCA 
AACCATCCGT TTTTCCGTCG TTCCGATTCA ATGACGTTCC TTGGTTGTAT TCCACCAAAC 
CCCTTTGAAG TTCCTCTGGC AGAAGCTATC CCACTTGCAG ACCAGCCCCA TCTTTTGCAG 
CCAAATGCAA GGAAAGAAGA CTTATTTGGT CGACCGAGTC AAGGTCTCTA CTCATCTACT 
GCTAACACTG GAAAATGCCT ATTAGAAGTG ACAGTTGACA GAAACTGCCT GGAGGTTTTA 
CCAACCAAAA TGTCATATGC TGCTAATCTA AAAAATGTAA TGAACATGCA GAGCCGTCAG 
AAAGAAGCAG AGGAACAGAA TGTGCCGGCT GAGGAAAATG ACACATCAAA GGCTGGACCC 
TCAGCTCATG ATCTTGCAGC TCAGCTGAAA AGTAGCTTGT TGGCAGAAAT AGGGTTAACA 
GAGAGTGAAG GTCCCCCGCT TACATCATTC AGGCCACAGT GTAGCTTTAT GGGAATGGTC 
ATTTCCCATG ATATGCTGTT AGGACGTTGG CGACTATCTC TTGAATTGTT TGGAAGGGTG 
TTTATGGAAG ATGTCGGAGC AGAGCCTGGA TCGATCTTAA CTGAACTAGG AGGGTTTGAA 
GTAAAGGAAT CCAAGTTCCG CAGGGAGATG GAAAAACTTC GAAATCAGCA GTCCAGAGAT 
CTTACACTAG AGGTGGATCG AGATAGAGAT CTTCTAATAC AGCAAACGAT GAGGCAGCTG 
AATAATCATT TTGGGCGCAG ATGCGCCACA ACTCCTATGG CAGTGCATAG AGTAAAGGTC 
ACCTTTAAAG ATGAGCCAGG AGAAGGCAGT GGAGTAGCCC GAAGTTTTTA TACTGCAGTA 
GCACAAGCAT TTTTGTCCAA TGAAAAGTTG CCAAGCTTGG ACTGTATCCA AAATGCCAAC 
AAAGGTACTC ATACAAGTTT GATGCAAAGA CTCAGAAATC GTGGGGAACG AGACCGGGAA 
AGGGAGCGGG ACATGAGAAG AAGCAGTGGT TTGCGAACAG GTTCTCGGCG GGATAGAGAC 
AGAGATTTCA GACGGCAACT TTCCATTGAT ACTAGGCCTT TCAGGCCAGC TTCTGAGGGC 
AATCCTAGTG ATGATCCTGA TCCTCTTCCT GCTCACCGAC AGGCTCTCGG AGAAAGACTA 
TACCCACGTG TACAAGCCAT GCAGCCGGCA TTTGCAAGTA AAATTACAGG CATGCTGCTT 
GAGCTATCTC CTGCACAACT GCTGCTGCTG TTGGCAAGTG AAGACTCTCT AAGAGCCAGA 
GTGGATGAAG CTATGGAACT TATTATTGCA CATGGAAGGG AGAATGGTGC TGACAGTATC 
CTCGATTTAG GTTTATTAGA AGCAGCTGAT AAGGTACAGG AAAACCGTAA GCGCCATGGC 
TCTAGTCGAA GCGTGGTTGA TATGGAGCTT ACTGACACAG ACGATGGGGA CGATAATGCT 
CCTTTATTTT ACCAACCTGG TAAACGAGGT TTCTATAGTC CAAGGCCTGG AAAAAACACA 
GAGGCACGTT TAAACTGTTT CCGCAACATC GGCAGAATTC TTGGTCTGTG CCTACTACAG 
AATGAATTAT GTCCTATCAC TTTAAGTAGG CAGGTGATTA AAGTGCTTCT GGGCAGAAAG 
GTGAACTGGC ATGATTTTGC GTTCTTTGAT CACGTGATGT ACGAGAGCTT ACGGCAACTT 
ATACTCGCCT CCCAAAGCCC GGATGCTGAC GCAGTGTTTG CCTCAATGGA CCTAGCGTTT 
GCAATAGACC TCTGCAAAGA AGAAGGTGGT GGACAAGTGG AACTTATTCC TAATGGCGTA 
AATATCCCTG TAACCCCTCA AAATGTGTAT GAATATGTAA GAAAATATGC TGAACACAGA 
ATGCTCACTG TAGCAGAGCA ACCGCTTAAT GCCATGAGGA AAGGACTATT GGATGTTCTC 
CCAAAGAATG CATTAGAAGA CTTAACTGCA GAAGACTTTA GGCTCTTAGT GAATGGCTGT 
GGGGAAGTTA ATGTTCAGAT GCTCATTAGC TTTACCTCCT TCAACGATGA GTCAGGAGAA 
AACGCAGAAA AGCTGTTGCA GTTCAAACGC TGGTTCTGGT CAATCGTAGA GAAGATGAGT 
ATGACAGAGC GGCAAGATTT AGTTTATTTT TGGACCTCAA GCCCATCATT GCCTGCGAGT 
GAAGAGGGAT TCCAGCCCAT GCCATCCATT ACCATCCGGC CCCCCGATGA TCAGCACCTT 
CCCACGGCGA ACACCTGCAT CTCTCGCCTC TACGTCCCCC TTTATTCTTC CAAACAGATC 
CTGAAACAGA AGTTGTTACT TGCTATTAAG ACCAAGAACT TTGGATTTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018079387.1
CDS	438..8831
Translation	MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLSSHPPLNVLEQTTIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELSKPENNDGSKLSSGSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGEDLMSLLDADIHSAHPSVIIDADAMFSEDISYFGYPSFRRSSLSRIGSSRVLLLPLERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPTLDKKNTPIMSPVSLGEDLQWWPEKDGTKFISIGALYSELVAVSSKGELYQWKWIEVEPYRNTQNPSLRHPRTSFLGLTNEKIVLLSANSIRATVATESNKVATWVDETLSSVASKLEHTAQIFPELQGDKIVSLHCCALYTCAQLENNLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNLEKTNKTIEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEERVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSNNSNNSNNSNNPCTPGSDPDPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGTWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLTNLPANSTIKKKAAIIIMAVEKQTLMQHILRGDYEACRQYLMNLEQGVVLDPNTQMLQTFLSHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTASTVPGQGPNTSTIPGPSSEPSVVESKDRKANAHFILKLLCDSIVLQPYLRDLLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEANSSEKEEDIFKGMVCPPGTNADDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTITNLVTMPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKAANPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPASSRIGHLLPEEQVYLNQQSGAIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAVAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSNSSNQSQSSYIIRNPPQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENTEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDLDQETFMIDEPLERSSNSNHANGAAQAPRSMQWAVRNTQSQRAPNTTPSNTSTPAASSGALIYIEPSSLRRTGNISSSAAAAAAALEASNSSSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLNYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALSSAGDPGHPNHPLHASQNSNRRERMTAREEASLRTLEGRRRATLLTARQGMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGLDNEDSEHENDDDTNQSLILNDKDDDTLPSETGPNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSTANTGKCLLEVTVDRNCLEVLPTKMSYAANLKNVMNMQSRQKEAEEQNVPAEENDTSKAGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLTLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAVAQAFLSNEKLPSLDCIQNANKGTHTSLMQRLRNRGERDRERERDMRRSSGLRTGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPDPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLLEAADKVQENRKRHGSSRSVVDMELTDTDDGDDNAPLFYQPGKRGFYSPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLSRQVIKVLLGRKVNWHDFAFFDHVMYESLRQLILASQSPDADAVFASMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLTVAEQPLNAMRKGLLDVLPKNALEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV

Target ORF information:

RefSeq Version	XM_018223898.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018223898.1

ORF Insert Sequence:

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAAGA TCTTATGTCA CTGCTGGATG CTGACATTCA TTCTGCTCAC 
CCAAGTGTCA TAATTGATGC TGATGCTATG TTTTCAGAAG ATATTAGCTA CTTTGGTTAT 
CCATCTTTTC GGCGCTCTTC ACTTTCCAGG ATAGGCTCAT CCAGAGTTCT CCTTCTTCCC 
TTAGAGAGAG ACTCTGAGCT GTTGCGTGAA CGTGAGTCAG TGTTGCGTTT GCGTGAGCGG 
AGGTGGCTTG ATGGGGCCTC GTTTGATAAT GAAAGAGGCT CCACAAGTAA AGAAGGGGAA 
CCTACACTCG ATAAGAAGAA CACTCCCATT ATGAGCCCAG TGTCACTGGG AGAAGACCTG 
CAATGGTGGC CTGAAAAGGA TGGGACAAAG TTTATTTCTA TTGGAGCACT TTATTCCGAG 
CTGGTTGCTG TAAGCAGTAA GGGAGAACTT TATCAGTGGA AGTGGATCGA GGTGGAGCCT 
TATAGGAATA CACAGAATCC ATCATTACGT CATCCAAGAA CATCCTTTTT AGGCCTAACA 
AATGAGAAAA TAGTCCTGTT GTCAGCAAAC AGTATCCGAG CCACAGTCGC TACAGAAAGC 
AACAAGGTTG CCACTTGGGT CGATGAAACA TTAAGCTCGG TAGCCTCAAA GTTAGAACAC 
ACAGCTCAGA TTTTCCCTGA GCTACAAGGA GACAAGATTG TCTCGTTGCA CTGTTGTGCA 
CTTTATACTT GTGCGCAATT AGAAAATAAC CTTTATTGGT GGGGTGTGGT TCCTTTCAGT 
CAGAGGAAGA AAATGCTTGA AAAAGCTCGT GCAAAGAATA AAAAACCCAA ATCTAGTGCC 
GGAATTTCAT CCATGCCAAA TATCACAGTT GGCACCCAGG TATGCTTAAG AAATAACCCT 
CTCTACCATG CGGGAGCTGT TGCTTTCTCG ATCAGTGCTG GCATTCCCAA AGTAGGAGTA 
TTAATGGAAT CTGTGTGGAA TATGAATGAT AGCTGCAGAT TTCAGCTCCG TTCTCCAGAG 
AGTTTAAAAA ACTTGGAAAA AACAAATAAA ACAATTGAAG CCAAACCTGA GAGCAAACAG 
GAACCTGTAA AAACAGAGAT GGGCCCGCCC CCATCTCCCG CATCTACCTG TAGTGATGCC 
TCTTCAATTG CTAGCAGTGC CTCCATGCCA TACAAACGAA GACGATCAAC TCCTGCTCCT 
AAGGAAGAAG AGAGAGTGAA TGAAGAACAG TGGTCTCTCA GAGAAGTTGT TTTTGTAGAA 
GATGTTAAAA ATGTTCCTGT TGGGAAGGTA CTAAAAGTTG ATGGGGCCTA TGTTGCAGTC 
AAATTTCCTG GCACTTCTAA TAATTCTAAT AATTCTAATA ATTCTAACAA TCCGTGCACC 
CCTGGCTCTG ATCCAGACCC ATCCTCTCTC CTCCAGGATT GTAGATTACT ACGGATTGAT 
GAACTACAGG TAGTGAAAAC TGGGGGGACA CCCAAAGTCC CAGACTGTTT CCAAAGGACA 
CCTAAAAAGC TGTGCATTCC TGAAAAAACT GAGATCCTGG CAGTCAATGT AGATTCCAAA 
GGGGTTCACG CGGTACTGAA AACAGGGACA TGGGTACGGT ACTGTATCTT TGATCTTGCA 
ACTGGAAAAG CAGAACAGGA AAACAACTTC CCAACAAGTA GCATTGCATT TCTTGGCCAG 
AATGAGAGAA ATGTTGCTAT TTTTACAGCT GGGCAGGAAT CTCCCATCAT TCTTAGAGAT 
GGCAATGGTA CCATTTATCC CATGGCAAAA GATTGCATGG GTGGGATAAG AGATCCAGAC 
TGGCTTGACC TTCCTCCAAT AAGCAGTCTT GGAATGGGAG TTCATTCTTT AACCAATCTC 
CCTGCAAATT CAACAATTAA GAAGAAAGCA GCCATTATCA TAATGGCTGT TGAGAAGCAA 
ACACTGATGC AACACATTTT GCGCGGTGAT TATGAGGCCT GCAGGCAGTA TTTGATGAAC 
CTAGAGCAAG GTGTAGTTTT GGATCCAAAC ACTCAAATGC TGCAGACTTT TCTGAGCCAC 
AGATGTGATG GCAATAGGAA CATATTGCAT GCCTGCGTGT CCGTTTGCTT CCCAACAAGC 
AACAAGGAAA CAAAAGAAGA GGAGGAAGCA GAGCGCTCTG AAAGGAATAC ATTTGCAGAA 
AGACTGTCTG CAGTAGAAGC AATTGCCAAT GCTATATCTG TGGTTTCTAG CAATGGTCCC 
GGAAATCGTG CTGGATCGTC CAGCAGTAGA AGCTTGAGAT TGAGGGAAAT GATGAGGAGG 
TCCCTTCGGG CAGCAGGTTT AGGAAGACAT GAGGCTGGTG CTTCTAGTGA TCACCAAGAC 
CCAGTATCTC CTCCCATTGC ACCTCCTAGC TGGGTTCCTG ATCCACCTGC TATGGATCCA 
GATGGTGACA TAGATTTTAT CCTGGCCCCT GCTGTAGGAT CTCTTACCAC TGCATCCACT 
GTACCTGGCC AAGGACCAAA TACTTCTACA ATACCAGGTC CTTCCTCAGA GCCATCAGTT 
GTTGAGTCCA AGGATCGGAA GGCAAATGCT CACTTCATAC TGAAGTTATT ATGCGATAGC 
ATCGTTCTGC AGCCTTACCT ACGTGATCTC CTGTCTGCCA AGGATGCAAG AGGTATGACT 
CCCTTCATGT CTGCTGTTAG TGGAAGAGCC TATCCAGCTG CAATTACCAT ACTGGAAACT 
GCACAGAAAA TCGCAAAAGC TGAAGCAAAT TCAAGTGAGA AAGAGGAGGA TATTTTCAAG 
GGAATGGTAT GTCCACCTGG TACCAATGCA GACGACTCTC CTCTATATGT ACTTTGCTGC 
AATGATACCT GCAGTTTTAC ATGGACAGGA GCTGAGCATA TCAATCAGGA CATATTTGAA 
TGTCGAACAT GTGGCTTGTT GGAATCCCTG TGCTGCTGTA CCGAGTGTGC AAGAGTCTGC 
CACAAAGGCC ATGACTGCAA ATTGAAAAGA ACATCACCTA CTGCTTACTG CGACTGTTGG 
GAGAAATGCA AATGCAAAAC ATTAATTGCC GGGCAAAAGT CTGCACGACT TGATCTGCTC 
TATCGACTGC TGACCATCAC TAATCTTGTT ACAATGCCAA ATAGCAGGGG AGAGCACCTA 
CTTCTTTTCT TAGTGCAGAC TGTAGCCAGA CAGACAGTGG AGCACTGCCA GTACCGACCT 
CCAAGGATTC GAGAAGATCG CAATCGAAAG GCAGCTAACC CTGAAGATTC CGACATGCCA 
GATCATGACC TAGAGCCCCC TAGGTTTGCC CAGCTTGCAC TGGAACGTGT TTTACAAGAT 
TGGAACGCAC TCAAATCCAT GATTATGTTT GGATCCCAAG AAAATAAAGA CCCTGCTAGC 
AGTAGAATCG GGCATCTTCT TCCAGAGGAA CAAGTTTACT TGAATCAGCA GAGTGGTGCC 
ATTCGCTTGG ACTGTTTTAC TCATTGCCTA ATTGTCAAGT GCACAGCAGA TATTTTGCTG 
TTGGATACCT TGCTGGGAAC CCTTGTAAAA GAACTCCAAA ATAAATATAC CCCTGGGCGT 
AGAGAAGAAG CTGTAGCTGT CACAATGCGT TTTCTGCGTT CAGTGGCTCG TGTTTTTGTC 
ATTCTCAGTG TAGAAATGGC TTCATCCAAG AAGAAAAACA ATTTCATTCC ACAGCCAATA 
GGGAAATGCA AGCGAGTGTT CCAAGCTTTA CTTCCATATG CAGTGGAGGA GTTGTGTAAT 
GTTGCAGAAT CCCTCATTGT CCCAGTGAGA ATGGGCATTG CACGCCCCAC TGCCCCCTTC 
ACATTAGCAA GTACAAGCAT AGATGCCATG CAGGGCAGCG AGGAGCTGTT CTCTGTGGAG 
CCTCTTCCTC CGAGACCTTC ATCTGATCAG TCTAACAGTT CGAATCAGTC CCAGTCATCC 
TATATCATTA GGAATCCTCC GCAAAGAAGA ATAAGCCAGT CCCAGCCTGT GCGAGGCCGA 
GATGAAGAGC AGGATGACAT TGTATCAGCA GATGTGGAAG AGGTTGAAGT GGTAGAGGGG 
GTAGCAGGAG AAGAGGACCA TCATGATGAG CAAGAAGAGC ATGGGGAGGA GAATACAGAA 
GCAGAAGGAC AACATGATGA GCACGATGAA GATGGTAGTG ACATGGAGTT AGACCTTCTT 
GCTGCAGCTG AAACAGAAAG TGACAGTGAA AGTAACCACA GCAATCAGGA TAATGCCAGC 
GGTCGGAGGA GTGTAGTAAC AGCAGCTACC GCTGGATCAG AAGCAGGTGC CAGCAGCGTT 
CCGGCCTTTT TCTCTGAAGA TGACTCCCAA TCCAATGACT CCAGTGATTC TGATAGCAGC 
AGCAGTCAAA GTGATGACCT GGACCAGGAG ACCTTTATGA TTGATGAGCC TCTAGAGAGG 
TCGTCCAACA GCAATCATGC AAATGGGGCT GCCCAGGCTC CAAGGTCAAT GCAGTGGGCA 
GTACGGAACA CTCAGAGCCA AAGAGCCCCA AACACCACAC CATCTAATAC TTCTACTCCA 
GCAGCCAGTT CTGGTGCCTT GATTTACATT GAACCATCCA GTCTGAGGCG CACTGGCAAC 
ATAAGCTCCA GTGCAGCAGC AGCAGCCGCA GCCCTTGAAG CAAGCAATTC TAGCAGTTAT 
CTAACTTCAG CAAGCAGTCT GGCTAGAGCC TACAGCATAG TGATACGGCA GATATCTGAC 
TTGATGGGTC TAATCCCCAA GTACAACCAC TTGGTCTATT CACAGATCCC AGCAGCCGTC 
AAACTGAACT ATCAAGATGC CGTTAACTTA CAGAACTATG TTGAAGAAAA ACTTATTCCC 
ACATGGAACT GGATGGTGAG CATAATGGAC TCCACTGAAG CCCAGCTCCG TTATGGCTCC 
GCCTTATCAT CCGCTGGAGA TCCAGGTCAT CCCAACCATC CTCTTCATGC ATCTCAGAAT 
TCCAACCGCA GGGAAAGGAT GACTGCCCGT GAGGAAGCTA GTTTGAGGAC TCTTGAGGGA 
AGAAGAAGAG CCACTCTGCT TACTGCTCGA CAGGGGATGT CGGCCCGTGG AGACTTCCTG 
AATTATGCAC TTTCACTAAT GCGTTCGCAC AATGATGAGC ACTCTGATGT ACTCCCTGTT 
TTGGATGTTT GCTCCCTGAA ACACGTGGCC TATGTCTTCC AGGCTCTGAT ATACTGGATT 
AAGGCAATGA ACCAACAAAC AACATTGGAT ACTCCACAGC TGGAAAGAAA GAGGACAAGA 
GAGCTTTTGG AGCTTGGCCT TGATAATGAA GATTCAGAGC ATGAAAATGA TGATGACACA 
AATCAAAGCC TAATATTGAA TGACAAAGAC GACGATACAC TACCCTCTGA GACCGGTCCA 
AACCATCCGT TTTTCCGTCG TTCCGATTCA ATGACGTTCC TTGGTTGTAT TCCACCAAAC 
CCCTTTGAAG TTCCTCTGGC AGAAGCTATC CCACTTGCAG ACCAGCCCCA TCTTTTGCAG 
CCAAATGCAA GGAAAGAAGA CTTATTTGGT CGACCGAGTC AAGGTCTCTA CTCATCTACT 
GCTAACACTG GAAAATGCCT ATTAGAAGTG ACAGTTGACA GAAACTGCCT GGAGGTTTTA 
CCAACCAAAA TGTCATATGC TGCTAATCTA AAAAATGTAA TGAACATGCA GAGCCGTCAG 
AAAGAAGCAG AGGAACAGAA TGTGCCGGCT GAGGAAAATG ACACATCAAA GGCTGGACCC 
TCAGCTCATG ATCTTGCAGC TCAGCTGAAA AGTAGCTTGT TGGCAGAAAT AGGGTTAACA 
GAGAGTGAAG GTCCCCCGCT TACATCATTC AGGCCACAGT GTAGCTTTAT GGGAATGGTC 
ATTTCCCATG ATATGCTGTT AGGACGTTGG CGACTATCTC TTGAATTGTT TGGAAGGGTG 
TTTATGGAAG ATGTCGGAGC AGAGCCTGGA TCGATCTTAA CTGAACTAGG AGGGTTTGAA 
GTAAAGGAAT CCAAGTTCCG CAGGGAGATG GAAAAACTTC GAAATCAGCA GTCCAGAGAT 
CTTACACTAG AGGTGGATCG AGATAGAGAT CTTCTAATAC AGCAAACGAT GAGGCAGCTG 
AATAATCATT TTGGGCGCAG ATGCGCCACA ACTCCTATGG CAGTGCATAG AGTAAAGGTC 
ACCTTTAAAG ATGAGCCAGG AGAAGGCAGT GGAGTAGCCC GAAGTTTTTA TACTGCAGTA 
GCACAAGCAT TTTTGTCCAA TGAAAAGTTG CCAAGCTTGG ACTGTATCCA AAATGCCAAC 
AAAGGTACTC ATACAAGTTT GATGCAAAGA CTCAGAAATC GTGGGGAACG AGACCGGGAA 
AGGGAGCGGG ACATGAGAAG AAGCAGTGGT TTGCGAACAG GTTCTCGGCG GGATAGAGAC 
AGAGATTTCA GACGGCAACT TTCCATTGAT ACTAGGCCTT TCAGGCCAGC TTCTGAGGGC 
AATCCTAGTG ATGATCCTGA TCCTCTTCCT GCTCACCGAC AGGCTCTCGG AGAAAGACTA 
TACCCACGTG TACAAGCCAT GCAGCCGGCA TTTGCAAGTA AAATTACAGG CATGCTGCTT 
GAGCTATCTC CTGCACAACT GCTGCTGCTG TTGGCAAGTG AAGACTCTCT AAGAGCCAGA 
GTGGATGAAG CTATGGAACT TATTATTGCA CATGGAAGGG AGAATGGTGC TGACAGTATC 
CTCGATTTAG GTTTATTAGA AGCAGCTGAT AAGGTACAGG AAAACCGTAA GCGCCATGGC 
TCTAGTCGAA GCGTGGTTGA TATGGAGCTT ACTGACACAG ACGATGGGGA CGATAATGCT 
CCTTTATTTT ACCAACCTGG TAAACGAGGT TTCTATAGTC CAAGGCCTGG AAAAAACACA 
GAGGCACGTT TAAACTGTTT CCGCAACATC GGCAGAATTC TTGGTCTGTG CCTACTACAG 
AATGAATTAT GTCCTATCAC TTTAAGTAGG CAGGTGATTA AAGTGCTTCT GGGCAGAAAG 
GTGAACTGGC ATGATTTTGC GTTCTTTGAT CACGTGATGT ACGAGAGCTT ACGGCAACTT 
ATACTCGCCT CCCAAAGCCC GGATGCTGAC GCAGTGTTTG CCTCAATGGA CCTAGCGTTT 
GCAATAGACC TCTGCAAAGA AGAAGGTGGT GGACAAGTGG AACTTATTCC TAATGGCGTA 
AATATCCCTG TAACCCCTCA AAATGTGTAT GAATATGTAA GAAAATATGC TGAACACAGA 
ATGCTCACTG TAGCAGAGCA ACCGCTTAAT GCCATGAGGA AAGGACTATT GGATGTTCTC 
CCAAAGAATG CATTAGAAGA CTTAACTGCA GAAGACTTTA GGCTCTTAGT GAATGGCTGT 
GGGGAAGTTA ATGTTCAGAT GCTCATTAGC TTTACCTCCT TCAACGATGA GTCAGGAGAA 
AACGCAGAAA AGCTGTTGCA GTTCAAACGC TGGTTCTGGT CAATCGTAGA GAAGATGAGT 
ATGACAGAGC GGCAAGATTT AGTTTATTTT TGGACCTCAA GCCCATCATT GCCTGCGAGT 
GAAGAGGGAT TCCAGCCCAT GCCATCCATT ACCATCCGGC CCCCCGATGA TCAGCACCTT 
CCCACGGCGA ACACCTGCAT CTCTCGCCTC TACGTCCCCC TTTATTCTTC CAAACAGATC 
CTGAAACAGA AGTTGTTACT TGCTATTAAG ACCAAGAACT TTGGATTTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108695408 ( XM_018223899.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018079388.1 Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X5, mRNA.

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CloneID	OAf37367
Clone ID Related Accession (Same CDS sequence)	XM_018223899.1
Accession Version	XM_018223899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase UBR5-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAAGA TCTTATGTCA CTGCTGGATG CTGACATTCA TTCTGCTCAC 
CCAAGTGTCA TAATTGATGC TGATGCTATG TTTTCAGAAG ATATTAGCTA CTTTGGTTAT 
CCATCTTTTC GGCGCTCTTC ACTTTCCAGG ATAGGCTCAT CCAGAGAGAG AGACTCTGAG 
CTGTTGCGTG AACGTGAGTC AGTGTTGCGT TTGCGTGAGC GGAGGTGGCT TGATGGGGCC 
TCGTTTGATA ATGAAAGAGG CTCCACAAGT AAAGAAGGGG AACCTACACT CGATAAGAAG 
AACACTCCCA TTATGAGCCC AGTGTCACTG GGAGAAGACC TGCAATGGTG GCCTGAAAAG 
GATGGGACAA AGTTTATTTC TATTGGAGCA CTTTATTCCG AGCTGGTTGC TGTAAGCAGT 
AAGGGAGAAC TTTATCAGTG GAAGTGGATC GAGGTGGAGC CTTATAGGAA TACACAGAAT 
CCATCATTAC GTCATCCAAG AACATCCTTT TTAGGCCTAA CAAATGAGAA AATAGTCCTG 
TTGTCAGCAA ACAGTATCCG AGCCACAGTC GCTACAGAAA GCAACAAGGT TGCCACTTGG 
GTCGATGAAA CATTAAGCTC GGTAGCCTCA AAGTTAGAAC ACACAGCTCA GATTTTCCCT 
GAGCTACAAG GAGACAAGAT TGTCTCGTTG CACTGTTGTG CACTTTATAC TTGTGCGCAA 
TTAGAAAATA ACCTTTATTG GTGGGGTGTG GTTCCTTTCA GTCAGAGGAA GAAAATGCTT 
GAAAAAGCTC GTGCAAAGAA TAAAAAACCC AAATCTAGTG CCGGAATTTC ATCCATGCCA 
AATATCACAG TTGGCACCCA GGTATGCTTA AGAAATAACC CTCTCTACCA TGCGGGAGCT 
GTTGCTTTCT CGATCAGTGC TGGCATTCCC AAAGTAGGAG TATTAATGGA ATCTGTGTGG 
AATATGAATG ATAGCTGCAG ATTTCAGCTC CGTTCTCCAG AGAGTTTAAA AAACTTGGAA 
AAAACAAATA AAACAATTGA AGCCAAACCT GAGAGCAAAC AGGAACCTGT AAAAACAGAG 
ATGGGCCCGC CCCCATCTCC CGCATCTACC TGTAGTGATG CCTCTTCAAT TGCTAGCAGT 
GCCTCCATGC CATACAAACG AAGACGATCA ACTCCTGCTC CTAAGGAAGA AGAGAGAGTG 
AATGAAGAAC AGTGGTCTCT CAGAGAAGTT GTTTTTGTAG AAGATGTTAA AAATGTTCCT 
GTTGGGAAGG TACTAAAAGT TGATGGGGCC TATGTTGCAG TCAAATTTCC TGGCACTTCT 
AATAATTCTA ATAATTCTAA TAATTCTAAC AATCCGTGCA CCCCTGGCTC TGATCCAGAC 
CCATCCTCTC TCCTCCAGGA TTGTAGATTA CTACGGATTG ATGAACTACA GGTAGTGAAA 
ACTGGGGGGA CACCCAAAGT CCCAGACTGT TTCCAAAGGA CACCTAAAAA GCTGTGCATT 
CCTGAAAAAA CTGAGATCCT GGCAGTCAAT GTAGATTCCA AAGGGGTTCA CGCGGTACTG 
AAAACAGGGA CATGGGTACG GTACTGTATC TTTGATCTTG CAACTGGAAA AGCAGAACAG 
GAAAACAACT TCCCAACAAG TAGCATTGCA TTTCTTGGCC AGAATGAGAG AAATGTTGCT 
ATTTTTACAG CTGGGCAGGA ATCTCCCATC ATTCTTAGAG ATGGCAATGG TACCATTTAT 
CCCATGGCAA AAGATTGCAT GGGTGGGATA AGAGATCCAG ACTGGCTTGA CCTTCCTCCA 
ATAAGCAGTC TTGGAATGGG AGTTCATTCT TTAACCAATC TCCCTGCAAA TTCAACAATT 
AAGAAGAAAG CAGCCATTAT CATAATGGCT GTTGAGAAGC AAACACTGAT GCAACACATT 
TTGCGCGGTG ATTATGAGGC CTGCAGGCAG TATTTGATGA ACCTAGAGCA AGGTGTAGTT 
TTGGATCCAA ACACTCAAAT GCTGCAGACT TTTCTGAGCC ACAGATGTGA TGGCAATAGG 
AACATATTGC ATGCCTGCGT GTCCGTTTGC TTCCCAACAA GCAACAAGGA AACAAAAGAA 
GAGGAGGAAG CAGAGCGCTC TGAAAGGAAT ACATTTGCAG AAAGACTGTC TGCAGTAGAA 
GCAATTGCCA ATGCTATATC TGTGGTTTCT AGCAATGGTC CCGGAAATCG TGCTGGATCG 
TCCAGCAGTA GAAGCTTGAG ATTGAGGGAA ATGATGAGGA GGTCCCTTCG GGCAGCAGGT 
TTAGGAAGAC ATGAGGCTGG TGCTTCTAGT GATCACCAAG ACCCAGTATC TCCTCCCATT 
GCACCTCCTA GCTGGGTTCC TGATCCACCT GCTATGGATC CAGATGGTGA CATAGATTTT 
ATCCTGGCCC CTGCTGTAGG ATCTCTTACC ACTGCATCCA CTGTACCTGG CCAAGGACCA 
AATACTTCTA CAATACCAGG TCCTTCCTCA GAGCCATCAG TTGTTGAGTC CAAGGATCGG 
AAGGCAAATG CTCACTTCAT ACTGAAGTTA TTATGCGATA GCATCGTTCT GCAGCCTTAC 
CTACGTGATC TCCTGTCTGC CAAGGATGCA AGAGGTATGA CTCCCTTCAT GTCTGCTGTT 
AGTGGAAGAG CCTATCCAGC TGCAATTACC ATACTGGAAA CTGCACAGAA AATCGCAAAA 
GCTGAAGCAA ATTCAAGTGA GAAAGAGGAG GATATTTTCA AGGGAATGGT ATGTCCACCT 
GGTACCAATG CAGACGACTC TCCTCTATAT GTACTTTGCT GCAATGATAC CTGCAGTTTT 
ACATGGACAG GAGCTGAGCA TATCAATCAG GACATATTTG AATGTCGAAC ATGTGGCTTG 
TTGGAATCCC TGTGCTGCTG TACCGAGTGT GCAAGAGTCT GCCACAAAGG CCATGACTGC 
AAATTGAAAA GAACATCACC TACTGCTTAC TGCGACTGTT GGGAGAAATG CAAATGCAAA 
ACATTAATTG CCGGGCAAAA GTCTGCACGA CTTGATCTGC TCTATCGACT GCTGACCATC 
ACTAATCTTG TTACAATGCC AAATAGCAGG GGAGAGCACC TACTTCTTTT CTTAGTGCAG 
ACTGTAGCCA GACAGACAGT GGAGCACTGC CAGTACCGAC CTCCAAGGAT TCGAGAAGAT 
CGCAATCGAA AGGCAGCTAA CCCTGAAGAT TCCGACATGC CAGATCATGA CCTAGAGCCC 
CCTAGGTTTG CCCAGCTTGC ACTGGAACGT GTTTTACAAG ATTGGAACGC ACTCAAATCC 
ATGATTATGT TTGGATCCCA AGAAAATAAA GACCCCCTTA GTGCTAGCAG TAGAATCGGG 
CATCTTCTTC CAGAGGAACA AGTTTACTTG AATCAGCAGA GTGGTGCCAT TCGCTTGGAC 
TGTTTTACTC ATTGCCTAAT TGTCAAGTGC ACAGCAGATA TTTTGCTGTT GGATACCTTG 
CTGGGAACCC TTGTAAAAGA ACTCCAAAAT AAATATACCC CTGGGCGTAG AGAAGAAGCT 
GTAGCTGTCA CAATGCGTTT TCTGCGTTCA GTGGCTCGTG TTTTTGTCAT TCTCAGTGTA 
GAAATGGCTT CATCCAAGAA GAAAAACAAT TTCATTCCAC AGCCAATAGG GAAATGCAAG 
CGAGTGTTCC AAGCTTTACT TCCATATGCA GTGGAGGAGT TGTGTAATGT TGCAGAATCC 
CTCATTGTCC CAGTGAGAAT GGGCATTGCA CGCCCCACTG CCCCCTTCAC ATTAGCAAGT 
ACAAGCATAG ATGCCATGCA GGGCAGCGAG GAGCTGTTCT CTGTGGAGCC TCTTCCTCCG 
AGACCTTCAT CTGATCAGTC TAACAGTTCG AATCAGTCCC AGTCATCCTA TATCATTAGG 
AATCCTCCGC AAAGAAGAAT AAGCCAGTCC CAGCCTGTGC GAGGCCGAGA TGAAGAGCAG 
GATGACATTG TATCAGCAGA TGTGGAAGAG GTTGAAGTGG TAGAGGGGGT AGCAGGAGAA 
GAGGACCATC ATGATGAGCA AGAAGAGCAT GGGGAGGAGA ATACAGAAGC AGAAGGACAA 
CATGATGAGC ACGATGAAGA TGGTAGTGAC ATGGAGTTAG ACCTTCTTGC TGCAGCTGAA 
ACAGAAAGTG ACAGTGAAAG TAACCACAGC AATCAGGATA ATGCCAGCGG TCGGAGGAGT 
GTAGTAACAG CAGCTACCGC TGGATCAGAA GCAGGTGCCA GCAGCGTTCC GGCCTTTTTC 
TCTGAAGATG ACTCCCAATC CAATGACTCC AGTGATTCTG ATAGCAGCAG CAGTCAAAGT 
GATGACCTGG ACCAGGAGAC CTTTATGATT GATGAGCCTC TAGAGAGGTC GTCCAACAGC 
AATCATGCAA ATGGGGCTGC CCAGGCTCCA AGGTCAATGC AGTGGGCAGT ACGGAACACT 
CAGAGCCAAA GAGCCCCAAA CACCACACCA TCTAATACTT CTACTCCAGC AGCCAGTTCT 
GGTGCCTTGA TTTACATTGA ACCATCCAGT CTGAGGCGCA CTGGCAACAT AAGCTCCAGT 
GCAGCAGCAG CAGCCGCAGC CCTTGAAGCA AGCAATTCTA GCAGTTATCT AACTTCAGCA 
AGCAGTCTGG CTAGAGCCTA CAGCATAGTG ATACGGCAGA TATCTGACTT GATGGGTCTA 
ATCCCCAAGT ACAACCACTT GGTCTATTCA CAGATCCCAG CAGCCGTCAA ACTGAACTAT 
CAAGATGCCG TTAACTTACA GAACTATGTT GAAGAAAAAC TTATTCCCAC ATGGAACTGG 
ATGGTGAGCA TAATGGACTC CACTGAAGCC CAGCTCCGTT ATGGCTCCGC CTTATCATCC 
GCTGGAGATC CAGGTCATCC CAACCATCCT CTTCATGCAT CTCAGAATTC CAACCGCAGG 
GAAAGGATGA CTGCCCGTGA GGAAGCTAGT TTGAGGACTC TTGAGGGAAG AAGAAGAGCC 
ACTCTGCTTA CTGCTCGACA GGGGATGTCG GCCCGTGGAG ACTTCCTGAA TTATGCACTT 
TCACTAATGC GTTCGCACAA TGATGAGCAC TCTGATGTAC TCCCTGTTTT GGATGTTTGC 
TCCCTGAAAC ACGTGGCCTA TGTCTTCCAG GCTCTGATAT ACTGGATTAA GGCAATGAAC 
CAACAAACAA CATTGGATAC TCCACAGCTG GAAAGAAAGA GGACAAGAGA GCTTTTGGAG 
CTTGGCCTTG ATAATGAAGA TTCAGAGCAT GAAAATGATG ATGACACAAA TCAAAGCCTA 
ATATTGAATG ACAAAGACGA CGATACACTA CCCTCTGAGA CCGGTCCAAA CCATCCGTTT 
TTCCGTCGTT CCGATTCAAT GACGTTCCTT GGTTGTATTC CACCAAACCC CTTTGAAGTT 
CCTCTGGCAG AAGCTATCCC ACTTGCAGAC CAGCCCCATC TTTTGCAGCC AAATGCAAGG 
AAAGAAGACT TATTTGGTCG ACCGAGTCAA GGTCTCTACT CATCTACTGC TAACACTGGA 
AAATGCCTAT TAGAAGTGAC AGTTGACAGA AACTGCCTGG AGGTTTTACC AACCAAAATG 
TCATATGCTG CTAATCTAAA AAATGTAATG AACATGCAGA GCCGTCAGAA AGAAGCAGAG 
GAACAGAATG TGCCGGCTGA GGAAAATGAC ACATCAAAGG CTGGACCCTC AGCTCATGAT 
CTTGCAGCTC AGCTGAAAAG TAGCTTGTTG GCAGAAATAG GGTTAACAGA GAGTGAAGGT 
CCCCCGCTTA CATCATTCAG GCCACAGTGT AGCTTTATGG GAATGGTCAT TTCCCATGAT 
ATGCTGTTAG GACGTTGGCG ACTATCTCTT GAATTGTTTG GAAGGGTGTT TATGGAAGAT 
GTCGGAGCAG AGCCTGGATC GATCTTAACT GAACTAGGAG GGTTTGAAGT AAAGGAATCC 
AAGTTCCGCA GGGAGATGGA AAAACTTCGA AATCAGCAGT CCAGAGATCT TACACTAGAG 
GTGGATCGAG ATAGAGATCT TCTAATACAG CAAACGATGA GGCAGCTGAA TAATCATTTT 
GGGCGCAGAT GCGCCACAAC TCCTATGGCA GTGCATAGAG TAAAGGTCAC CTTTAAAGAT 
GAGCCAGGAG AAGGCAGTGG AGTAGCCCGA AGTTTTTATA CTGCAGTAGC ACAAGCATTT 
TTGTCCAATG AAAAGTTGCC AAGCTTGGAC TGTATCCAAA ATGCCAACAA AGGTACTCAT 
ACAAGTTTGA TGCAAAGACT CAGAAATCGT GGGGAACGAG ACCGGGAAAG GGAGCGGGAC 
ATGAGAAGAA GCAGTGGTTT GCGAACAGGT TCTCGGCGGG ATAGAGACAG AGATTTCAGA 
CGGCAACTTT CCATTGATAC TAGGCCTTTC AGGCCAGCTT CTGAGGGCAA TCCTAGTGAT 
GATCCTGATC CTCTTCCTGC TCACCGACAG GCTCTCGGAG AAAGACTATA CCCACGTGTA 
CAAGCCATGC AGCCGGCATT TGCAAGTAAA ATTACAGGCA TGCTGCTTGA GCTATCTCCT 
GCACAACTGC TGCTGCTGTT GGCAAGTGAA GACTCTCTAA GAGCCAGAGT GGATGAAGCT 
ATGGAACTTA TTATTGCACA TGGAAGGGAG AATGGTGCTG ACAGTATCCT CGATTTAGGT 
TTATTAGAAG CAGCTGATAA GGTACAGGAA AACCGTAAGC GCCATGGCTC TAGTCGAAGC 
GTGGTTGATA TGGAGCTTAC TGACACAGAC GATGGGGACG ATAATGCTCC TTTATTTTAC 
CAACCTGGTA AACGAGGTTT CTATAGTCCA AGGCCTGGAA AAAACACAGA GGCACGTTTA 
AACTGTTTCC GCAACATCGG CAGAATTCTT GGTCTGTGCC TACTACAGAA TGAATTATGT 
CCTATCACTT TAAGTAGGCA GGTGATTAAA GTGCTTCTGG GCAGAAAGGT GAACTGGCAT 
GATTTTGCGT TCTTTGATCA CGTGATGTAC GAGAGCTTAC GGCAACTTAT ACTCGCCTCC 
CAAAGCCCGG ATGCTGACGC AGTGTTTGCC TCAATGGACC TAGCGTTTGC AATAGACCTC 
TGCAAAGAAG AAGGTGGTGG ACAAGTGGAA CTTATTCCTA ATGGCGTAAA TATCCCTGTA 
ACCCCTCAAA ATGTGTATGA ATATGTAAGA AAATATGCTG AACACAGAAT GCTCACTGTA 
GCAGAGCAAC CGCTTAATGC CATGAGGAAA GGACTATTGG ATGTTCTCCC AAAGAATGCA 
TTAGAAGACT TAACTGCAGA AGACTTTAGG CTCTTAGTGA ATGGCTGTGG GGAAGTTAAT 
GTTCAGATGC TCATTAGCTT TACCTCCTTC AACGATGAGT CAGGAGAAAA CGCAGAAAAG 
CTGTTGCAGT TCAAACGCTG GTTCTGGTCA ATCGTAGAGA AGATGAGTAT GACAGAGCGG 
CAAGATTTAG TTTATTTTTG GACCTCAAGC CCATCATTGC CTGCGAGTGA AGAGGGATTC 
CAGCCCATGC CATCCATTAC CATCCGGCCC CCCGATGATC AGCACCTTCC CACGGCGAAC 
ACCTGCATCT CTCGCCTCTA CGTCCCCCTT TATTCTTCCA AACAGATCCT GAAACAGAAG 
TTGTTACTTG CTATTAAGAC CAAGAACTTT GGATTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018079388.1
CDS	438..8819
Translation	MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLSSHPPLNVLEQTTIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELSKPENNDGSKLSSGSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGEDLMSLLDADIHSAHPSVIIDADAMFSEDISYFGYPSFRRSSLSRIGSSRERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPTLDKKNTPIMSPVSLGEDLQWWPEKDGTKFISIGALYSELVAVSSKGELYQWKWIEVEPYRNTQNPSLRHPRTSFLGLTNEKIVLLSANSIRATVATESNKVATWVDETLSSVASKLEHTAQIFPELQGDKIVSLHCCALYTCAQLENNLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNLEKTNKTIEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEERVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSNNSNNSNNSNNPCTPGSDPDPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGTWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLTNLPANSTIKKKAAIIIMAVEKQTLMQHILRGDYEACRQYLMNLEQGVVLDPNTQMLQTFLSHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTASTVPGQGPNTSTIPGPSSEPSVVESKDRKANAHFILKLLCDSIVLQPYLRDLLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEANSSEKEEDIFKGMVCPPGTNADDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTITNLVTMPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKAANPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGAIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAVAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSNSSNQSQSSYIIRNPPQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENTEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDLDQETFMIDEPLERSSNSNHANGAAQAPRSMQWAVRNTQSQRAPNTTPSNTSTPAASSGALIYIEPSSLRRTGNISSSAAAAAAALEASNSSSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLNYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALSSAGDPGHPNHPLHASQNSNRRERMTAREEASLRTLEGRRRATLLTARQGMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGLDNEDSEHENDDDTNQSLILNDKDDDTLPSETGPNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSTANTGKCLLEVTVDRNCLEVLPTKMSYAANLKNVMNMQSRQKEAEEQNVPAEENDTSKAGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLTLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAVAQAFLSNEKLPSLDCIQNANKGTHTSLMQRLRNRGERDRERERDMRRSSGLRTGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPDPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLLEAADKVQENRKRHGSSRSVVDMELTDTDDGDDNAPLFYQPGKRGFYSPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLSRQVIKVLLGRKVNWHDFAFFDHVMYESLRQLILASQSPDADAVFASMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLTVAEQPLNAMRKGLLDVLPKNALEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV

Target ORF information:

RefSeq Version	XM_018223899.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018223899.1

ORF Insert Sequence:

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAAGA TCTTATGTCA CTGCTGGATG CTGACATTCA TTCTGCTCAC 
CCAAGTGTCA TAATTGATGC TGATGCTATG TTTTCAGAAG ATATTAGCTA CTTTGGTTAT 
CCATCTTTTC GGCGCTCTTC ACTTTCCAGG ATAGGCTCAT CCAGAGAGAG AGACTCTGAG 
CTGTTGCGTG AACGTGAGTC AGTGTTGCGT TTGCGTGAGC GGAGGTGGCT TGATGGGGCC 
TCGTTTGATA ATGAAAGAGG CTCCACAAGT AAAGAAGGGG AACCTACACT CGATAAGAAG 
AACACTCCCA TTATGAGCCC AGTGTCACTG GGAGAAGACC TGCAATGGTG GCCTGAAAAG 
GATGGGACAA AGTTTATTTC TATTGGAGCA CTTTATTCCG AGCTGGTTGC TGTAAGCAGT 
AAGGGAGAAC TTTATCAGTG GAAGTGGATC GAGGTGGAGC CTTATAGGAA TACACAGAAT 
CCATCATTAC GTCATCCAAG AACATCCTTT TTAGGCCTAA CAAATGAGAA AATAGTCCTG 
TTGTCAGCAA ACAGTATCCG AGCCACAGTC GCTACAGAAA GCAACAAGGT TGCCACTTGG 
GTCGATGAAA CATTAAGCTC GGTAGCCTCA AAGTTAGAAC ACACAGCTCA GATTTTCCCT 
GAGCTACAAG GAGACAAGAT TGTCTCGTTG CACTGTTGTG CACTTTATAC TTGTGCGCAA 
TTAGAAAATA ACCTTTATTG GTGGGGTGTG GTTCCTTTCA GTCAGAGGAA GAAAATGCTT 
GAAAAAGCTC GTGCAAAGAA TAAAAAACCC AAATCTAGTG CCGGAATTTC ATCCATGCCA 
AATATCACAG TTGGCACCCA GGTATGCTTA AGAAATAACC CTCTCTACCA TGCGGGAGCT 
GTTGCTTTCT CGATCAGTGC TGGCATTCCC AAAGTAGGAG TATTAATGGA ATCTGTGTGG 
AATATGAATG ATAGCTGCAG ATTTCAGCTC CGTTCTCCAG AGAGTTTAAA AAACTTGGAA 
AAAACAAATA AAACAATTGA AGCCAAACCT GAGAGCAAAC AGGAACCTGT AAAAACAGAG 
ATGGGCCCGC CCCCATCTCC CGCATCTACC TGTAGTGATG CCTCTTCAAT TGCTAGCAGT 
GCCTCCATGC CATACAAACG AAGACGATCA ACTCCTGCTC CTAAGGAAGA AGAGAGAGTG 
AATGAAGAAC AGTGGTCTCT CAGAGAAGTT GTTTTTGTAG AAGATGTTAA AAATGTTCCT 
GTTGGGAAGG TACTAAAAGT TGATGGGGCC TATGTTGCAG TCAAATTTCC TGGCACTTCT 
AATAATTCTA ATAATTCTAA TAATTCTAAC AATCCGTGCA CCCCTGGCTC TGATCCAGAC 
CCATCCTCTC TCCTCCAGGA TTGTAGATTA CTACGGATTG ATGAACTACA GGTAGTGAAA 
ACTGGGGGGA CACCCAAAGT CCCAGACTGT TTCCAAAGGA CACCTAAAAA GCTGTGCATT 
CCTGAAAAAA CTGAGATCCT GGCAGTCAAT GTAGATTCCA AAGGGGTTCA CGCGGTACTG 
AAAACAGGGA CATGGGTACG GTACTGTATC TTTGATCTTG CAACTGGAAA AGCAGAACAG 
GAAAACAACT TCCCAACAAG TAGCATTGCA TTTCTTGGCC AGAATGAGAG AAATGTTGCT 
ATTTTTACAG CTGGGCAGGA ATCTCCCATC ATTCTTAGAG ATGGCAATGG TACCATTTAT 
CCCATGGCAA AAGATTGCAT GGGTGGGATA AGAGATCCAG ACTGGCTTGA CCTTCCTCCA 
ATAAGCAGTC TTGGAATGGG AGTTCATTCT TTAACCAATC TCCCTGCAAA TTCAACAATT 
AAGAAGAAAG CAGCCATTAT CATAATGGCT GTTGAGAAGC AAACACTGAT GCAACACATT 
TTGCGCGGTG ATTATGAGGC CTGCAGGCAG TATTTGATGA ACCTAGAGCA AGGTGTAGTT 
TTGGATCCAA ACACTCAAAT GCTGCAGACT TTTCTGAGCC ACAGATGTGA TGGCAATAGG 
AACATATTGC ATGCCTGCGT GTCCGTTTGC TTCCCAACAA GCAACAAGGA AACAAAAGAA 
GAGGAGGAAG CAGAGCGCTC TGAAAGGAAT ACATTTGCAG AAAGACTGTC TGCAGTAGAA 
GCAATTGCCA ATGCTATATC TGTGGTTTCT AGCAATGGTC CCGGAAATCG TGCTGGATCG 
TCCAGCAGTA GAAGCTTGAG ATTGAGGGAA ATGATGAGGA GGTCCCTTCG GGCAGCAGGT 
TTAGGAAGAC ATGAGGCTGG TGCTTCTAGT GATCACCAAG ACCCAGTATC TCCTCCCATT 
GCACCTCCTA GCTGGGTTCC TGATCCACCT GCTATGGATC CAGATGGTGA CATAGATTTT 
ATCCTGGCCC CTGCTGTAGG ATCTCTTACC ACTGCATCCA CTGTACCTGG CCAAGGACCA 
AATACTTCTA CAATACCAGG TCCTTCCTCA GAGCCATCAG TTGTTGAGTC CAAGGATCGG 
AAGGCAAATG CTCACTTCAT ACTGAAGTTA TTATGCGATA GCATCGTTCT GCAGCCTTAC 
CTACGTGATC TCCTGTCTGC CAAGGATGCA AGAGGTATGA CTCCCTTCAT GTCTGCTGTT 
AGTGGAAGAG CCTATCCAGC TGCAATTACC ATACTGGAAA CTGCACAGAA AATCGCAAAA 
GCTGAAGCAA ATTCAAGTGA GAAAGAGGAG GATATTTTCA AGGGAATGGT ATGTCCACCT 
GGTACCAATG CAGACGACTC TCCTCTATAT GTACTTTGCT GCAATGATAC CTGCAGTTTT 
ACATGGACAG GAGCTGAGCA TATCAATCAG GACATATTTG AATGTCGAAC ATGTGGCTTG 
TTGGAATCCC TGTGCTGCTG TACCGAGTGT GCAAGAGTCT GCCACAAAGG CCATGACTGC 
AAATTGAAAA GAACATCACC TACTGCTTAC TGCGACTGTT GGGAGAAATG CAAATGCAAA 
ACATTAATTG CCGGGCAAAA GTCTGCACGA CTTGATCTGC TCTATCGACT GCTGACCATC 
ACTAATCTTG TTACAATGCC AAATAGCAGG GGAGAGCACC TACTTCTTTT CTTAGTGCAG 
ACTGTAGCCA GACAGACAGT GGAGCACTGC CAGTACCGAC CTCCAAGGAT TCGAGAAGAT 
CGCAATCGAA AGGCAGCTAA CCCTGAAGAT TCCGACATGC CAGATCATGA CCTAGAGCCC 
CCTAGGTTTG CCCAGCTTGC ACTGGAACGT GTTTTACAAG ATTGGAACGC ACTCAAATCC 
ATGATTATGT TTGGATCCCA AGAAAATAAA GACCCCCTTA GTGCTAGCAG TAGAATCGGG 
CATCTTCTTC CAGAGGAACA AGTTTACTTG AATCAGCAGA GTGGTGCCAT TCGCTTGGAC 
TGTTTTACTC ATTGCCTAAT TGTCAAGTGC ACAGCAGATA TTTTGCTGTT GGATACCTTG 
CTGGGAACCC TTGTAAAAGA ACTCCAAAAT AAATATACCC CTGGGCGTAG AGAAGAAGCT 
GTAGCTGTCA CAATGCGTTT TCTGCGTTCA GTGGCTCGTG TTTTTGTCAT TCTCAGTGTA 
GAAATGGCTT CATCCAAGAA GAAAAACAAT TTCATTCCAC AGCCAATAGG GAAATGCAAG 
CGAGTGTTCC AAGCTTTACT TCCATATGCA GTGGAGGAGT TGTGTAATGT TGCAGAATCC 
CTCATTGTCC CAGTGAGAAT GGGCATTGCA CGCCCCACTG CCCCCTTCAC ATTAGCAAGT 
ACAAGCATAG ATGCCATGCA GGGCAGCGAG GAGCTGTTCT CTGTGGAGCC TCTTCCTCCG 
AGACCTTCAT CTGATCAGTC TAACAGTTCG AATCAGTCCC AGTCATCCTA TATCATTAGG 
AATCCTCCGC AAAGAAGAAT AAGCCAGTCC CAGCCTGTGC GAGGCCGAGA TGAAGAGCAG 
GATGACATTG TATCAGCAGA TGTGGAAGAG GTTGAAGTGG TAGAGGGGGT AGCAGGAGAA 
GAGGACCATC ATGATGAGCA AGAAGAGCAT GGGGAGGAGA ATACAGAAGC AGAAGGACAA 
CATGATGAGC ACGATGAAGA TGGTAGTGAC ATGGAGTTAG ACCTTCTTGC TGCAGCTGAA 
ACAGAAAGTG ACAGTGAAAG TAACCACAGC AATCAGGATA ATGCCAGCGG TCGGAGGAGT 
GTAGTAACAG CAGCTACCGC TGGATCAGAA GCAGGTGCCA GCAGCGTTCC GGCCTTTTTC 
TCTGAAGATG ACTCCCAATC CAATGACTCC AGTGATTCTG ATAGCAGCAG CAGTCAAAGT 
GATGACCTGG ACCAGGAGAC CTTTATGATT GATGAGCCTC TAGAGAGGTC GTCCAACAGC 
AATCATGCAA ATGGGGCTGC CCAGGCTCCA AGGTCAATGC AGTGGGCAGT ACGGAACACT 
CAGAGCCAAA GAGCCCCAAA CACCACACCA TCTAATACTT CTACTCCAGC AGCCAGTTCT 
GGTGCCTTGA TTTACATTGA ACCATCCAGT CTGAGGCGCA CTGGCAACAT AAGCTCCAGT 
GCAGCAGCAG CAGCCGCAGC CCTTGAAGCA AGCAATTCTA GCAGTTATCT AACTTCAGCA 
AGCAGTCTGG CTAGAGCCTA CAGCATAGTG ATACGGCAGA TATCTGACTT GATGGGTCTA 
ATCCCCAAGT ACAACCACTT GGTCTATTCA CAGATCCCAG CAGCCGTCAA ACTGAACTAT 
CAAGATGCCG TTAACTTACA GAACTATGTT GAAGAAAAAC TTATTCCCAC ATGGAACTGG 
ATGGTGAGCA TAATGGACTC CACTGAAGCC CAGCTCCGTT ATGGCTCCGC CTTATCATCC 
GCTGGAGATC CAGGTCATCC CAACCATCCT CTTCATGCAT CTCAGAATTC CAACCGCAGG 
GAAAGGATGA CTGCCCGTGA GGAAGCTAGT TTGAGGACTC TTGAGGGAAG AAGAAGAGCC 
ACTCTGCTTA CTGCTCGACA GGGGATGTCG GCCCGTGGAG ACTTCCTGAA TTATGCACTT 
TCACTAATGC GTTCGCACAA TGATGAGCAC TCTGATGTAC TCCCTGTTTT GGATGTTTGC 
TCCCTGAAAC ACGTGGCCTA TGTCTTCCAG GCTCTGATAT ACTGGATTAA GGCAATGAAC 
CAACAAACAA CATTGGATAC TCCACAGCTG GAAAGAAAGA GGACAAGAGA GCTTTTGGAG 
CTTGGCCTTG ATAATGAAGA TTCAGAGCAT GAAAATGATG ATGACACAAA TCAAAGCCTA 
ATATTGAATG ACAAAGACGA CGATACACTA CCCTCTGAGA CCGGTCCAAA CCATCCGTTT 
TTCCGTCGTT CCGATTCAAT GACGTTCCTT GGTTGTATTC CACCAAACCC CTTTGAAGTT 
CCTCTGGCAG AAGCTATCCC ACTTGCAGAC CAGCCCCATC TTTTGCAGCC AAATGCAAGG 
AAAGAAGACT TATTTGGTCG ACCGAGTCAA GGTCTCTACT CATCTACTGC TAACACTGGA 
AAATGCCTAT TAGAAGTGAC AGTTGACAGA AACTGCCTGG AGGTTTTACC AACCAAAATG 
TCATATGCTG CTAATCTAAA AAATGTAATG AACATGCAGA GCCGTCAGAA AGAAGCAGAG 
GAACAGAATG TGCCGGCTGA GGAAAATGAC ACATCAAAGG CTGGACCCTC AGCTCATGAT 
CTTGCAGCTC AGCTGAAAAG TAGCTTGTTG GCAGAAATAG GGTTAACAGA GAGTGAAGGT 
CCCCCGCTTA CATCATTCAG GCCACAGTGT AGCTTTATGG GAATGGTCAT TTCCCATGAT 
ATGCTGTTAG GACGTTGGCG ACTATCTCTT GAATTGTTTG GAAGGGTGTT TATGGAAGAT 
GTCGGAGCAG AGCCTGGATC GATCTTAACT GAACTAGGAG GGTTTGAAGT AAAGGAATCC 
AAGTTCCGCA GGGAGATGGA AAAACTTCGA AATCAGCAGT CCAGAGATCT TACACTAGAG 
GTGGATCGAG ATAGAGATCT TCTAATACAG CAAACGATGA GGCAGCTGAA TAATCATTTT 
GGGCGCAGAT GCGCCACAAC TCCTATGGCA GTGCATAGAG TAAAGGTCAC CTTTAAAGAT 
GAGCCAGGAG AAGGCAGTGG AGTAGCCCGA AGTTTTTATA CTGCAGTAGC ACAAGCATTT 
TTGTCCAATG AAAAGTTGCC AAGCTTGGAC TGTATCCAAA ATGCCAACAA AGGTACTCAT 
ACAAGTTTGA TGCAAAGACT CAGAAATCGT GGGGAACGAG ACCGGGAAAG GGAGCGGGAC 
ATGAGAAGAA GCAGTGGTTT GCGAACAGGT TCTCGGCGGG ATAGAGACAG AGATTTCAGA 
CGGCAACTTT CCATTGATAC TAGGCCTTTC AGGCCAGCTT CTGAGGGCAA TCCTAGTGAT 
GATCCTGATC CTCTTCCTGC TCACCGACAG GCTCTCGGAG AAAGACTATA CCCACGTGTA 
CAAGCCATGC AGCCGGCATT TGCAAGTAAA ATTACAGGCA TGCTGCTTGA GCTATCTCCT 
GCACAACTGC TGCTGCTGTT GGCAAGTGAA GACTCTCTAA GAGCCAGAGT GGATGAAGCT 
ATGGAACTTA TTATTGCACA TGGAAGGGAG AATGGTGCTG ACAGTATCCT CGATTTAGGT 
TTATTAGAAG CAGCTGATAA GGTACAGGAA AACCGTAAGC GCCATGGCTC TAGTCGAAGC 
GTGGTTGATA TGGAGCTTAC TGACACAGAC GATGGGGACG ATAATGCTCC TTTATTTTAC 
CAACCTGGTA AACGAGGTTT CTATAGTCCA AGGCCTGGAA AAAACACAGA GGCACGTTTA 
AACTGTTTCC GCAACATCGG CAGAATTCTT GGTCTGTGCC TACTACAGAA TGAATTATGT 
CCTATCACTT TAAGTAGGCA GGTGATTAAA GTGCTTCTGG GCAGAAAGGT GAACTGGCAT 
GATTTTGCGT TCTTTGATCA CGTGATGTAC GAGAGCTTAC GGCAACTTAT ACTCGCCTCC 
CAAAGCCCGG ATGCTGACGC AGTGTTTGCC TCAATGGACC TAGCGTTTGC AATAGACCTC 
TGCAAAGAAG AAGGTGGTGG ACAAGTGGAA CTTATTCCTA ATGGCGTAAA TATCCCTGTA 
ACCCCTCAAA ATGTGTATGA ATATGTAAGA AAATATGCTG AACACAGAAT GCTCACTGTA 
GCAGAGCAAC CGCTTAATGC CATGAGGAAA GGACTATTGG ATGTTCTCCC AAAGAATGCA 
TTAGAAGACT TAACTGCAGA AGACTTTAGG CTCTTAGTGA ATGGCTGTGG GGAAGTTAAT 
GTTCAGATGC TCATTAGCTT TACCTCCTTC AACGATGAGT CAGGAGAAAA CGCAGAAAAG 
CTGTTGCAGT TCAAACGCTG GTTCTGGTCA ATCGTAGAGA AGATGAGTAT GACAGAGCGG 
CAAGATTTAG TTTATTTTTG GACCTCAAGC CCATCATTGC CTGCGAGTGA AGAGGGATTC 
CAGCCCATGC CATCCATTAC CATCCGGCCC CCCGATGATC AGCACCTTCC CACGGCGAAC 
ACCTGCATCT CTCGCCTCTA CGTCCCCCTT TATTCTTCCA AACAGATCCT GAAACAGAAG 
TTGTTACTTG CTATTAAGAC CAAGAACTTT GGATTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108695408 ( XM_018223900.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018079389.1 Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X6, mRNA.

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CloneID	OAf37368
Clone ID Related Accession (Same CDS sequence)	XM_018223900.1
Accession Version	XM_018223900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase UBR5-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAAGA TCTTATGTCA CTGCTGGATG CTGACATTCA TTCTGCTCAC 
CCAAGTGTCA TAATTGATGC TGATGCTATG TTTTCAGAAG ATATTAGCTA CTTTGGTTAT 
CCATCTTTTC GGCGCTCTTC ACTTTCCAGG ATAGGCTCAT CCAGAGTTCT CCTTCTTCCC 
TTAGAGAGAG ACTCTGAGCT GTTGCGTGAA CGTGAGTCAG TGTTGCGTTT GCGTGAGCGG 
AGGTGGCTTG ATGGGGCCTC GTTTGATAAT GAAAGAGGCT CCACAAGTAA AGAAGGGGAA 
CCTACACTCG ATAAGAAGAA CACTCCCATT ATGAGCCCAG TGTCACTGGG AGAAGACCTG 
CAATGGTGGC CTGAAAAGGA TGGGACAAAG TTTATTTCTA TTGGAGCACT TTATTCCGAG 
CTGGTTGCTG TAAGCAGTAA GGGAGAACTT TATCAGTGGA AGTGGATCGA GGTGGAGCCT 
TATAGGAATA CACAGAATCC ATCATTACGT CATCCAAGAA CATCCTTTTT AGGCCTAACA 
AATGAGAAAA TAGTCCTGTT GTCAGCAAAC AGTATCCGAG CCACAGTCGC TACAGAAAGC 
AACAAGGTTG CCACTTGGGT CGATGAAACA TTAAGCTCGG TAGCCTCAAA GTTAGAACAC 
ACAGCTCAGA TTTTCCCTGA GCTACAAGGA GACAAGATTG TCTCGTTGCA CTGTTGTGCA 
CTTTATACTT GTGCGCAATT AGAAAATAAC CTTTATTGGT GGGGTGTGGT TCCTTTCAGT 
CAGAGGAAGA AAATGCTTGA AAAAGCTCGT GCAAAGAATA AAAAACCCAA ATCTAGTGCC 
GGAATTTCAT CCATGCCAAA TATCACAGTT GGCACCCAGG TATGCTTAAG AAATAACCCT 
CTCTACCATG CGGGAGCTGT TGCTTTCTCG ATCAGTGCTG GCATTCCCAA AGTAGGAGTA 
TTAATGGAAT CTGTGTGGAA TATGAATGAT AGCTGCAGAT TTCAGCTCCG TTCTCCAGAG 
AGTTTAAAAA ACTTGGAAAA AACAAATAAA ACAATTGAAG CCAAACCTGA GAGCAAACAG 
GAACCTGTAA AAACAGAGAT GGGCCCGCCC CCATCTCCCG CATCTACCTG TAGTGATGCC 
TCTTCAATTG CTAGCAGTGC CTCCATGCCA TACAAACGAA GACGATCAAC TCCTGCTCCT 
AAGGAAGAAG AGAGAGTGAA TGAAGAACAG TGGTCTCTCA GAGAAGTTGT TTTTGTAGAA 
GATGTTAAAA ATGTTCCTGT TGGGAAGGTA CTAAAAGTTG ATGGGGCCTA TGTTGCAGTC 
AAATTTCCTG GCACTTCTAA TAATTCTAAT AATTCTAATA ATTCTAACAA TCCGTGCACC 
CCTGGCTCTG ATCCAGACCC ATCCTCTCTC CTCCAGGATT GTAGATTACT ACGGATTGAT 
GAACTACAGG TAGTGAAAAC TGGGGGGACA CCCAAAGTCC CAGACTGTTT CCAAAGGACA 
CCTAAAAAGC TGTGCATTCC TGAAAAAACT GAGATCCTGG CAGTCAATGT AGATTCCAAA 
GGGGTTCACG CGGTACTGAA AACAGGGACA TGGGTACGGT ACTGTATCTT TGATCTTGCA 
ACTGGAAAAG CAGAACAGGA AAACAACTTC CCAACAAGTA GCATTGCATT TCTTGGCCAG 
AATGAGAGAA ATGTTGCTAT TTTTACAGCT GGGCAGGAAT CTCCCATCAT TCTTAGAGAT 
GGCAATGGTA CCATTTATCC CATGGCAAAA GATTGCATGG GTGGGATAAG AGATCCAGAC 
TGGCTTGACC TTCCTCCAAT AAGCAGTCTT GGAATGGGAG TTCATTCTTT AACCAATCTC 
CCTGCAAATT CAACAATTAA GAAGAAAGCA GCCATTATCA TAATGGCTGT TGAGAAGCAA 
ACACTGATGC AACACATTTT GCGCGGTGAT TATGAGGCCT GCAGGCAGTA TTTGATGAAC 
CTAGAGCAAG GTGTAGTTTT GGATCCAAAC ACTCAAATGC TGCAGACTTT TCTGAGCCAC 
AGATGTGATG GCAATAGGAA CATATTGCAT GCCTGCGTGT CCGTTTGCTT CCCAACAAGC 
AACAAGGAAA CAAAAGAAGA GGAGGAAGCA GAGCGCTCTG AAAGGAATAC ATTTGCAGAA 
AGACTGTCTG CAGTAGAAGC AATTGCCAAT GCTATATCTG TGGTTTCTAG CAATGGTCCC 
GGAAATCGTG CTGGATCGTC CAGCAGTAGA AGCTTGAGAT TGAGGGAAAT GATGAGGAGG 
TCCCTTCGGG CAGCAGGTTT AGGAAGACAT GAGGCTGGTG CTTCTAGTGA TCACCAAGAC 
CCAGTATCTC CTCCCATTGC ACCTCCTAGC TGGGTTCCTG ATCCACCTGC TATGGATCCA 
GATGGTGACA TAGATTTTAT CCTGGCCCCT GCTGTAGGAT CTCTTACCAC TGCATCCACT 
GTACCTGGCC AAGGACCAAA TACTTCTACA ATACCAGGTC CTTCCTCAGA GCCATCAGTT 
GTTGAGTCCA AGGATCGGAA GGCAAATGCT CACTTCATAC TGAAGTTATT ATGCGATAGC 
ATCGTTCTGC AGCCTTACCT ACGTGATCTC CTGTCTGCCA AGGATGCAAG AGGTATGACT 
CCCTTCATGT CTGCTGTTAG TGGAAGAGCC TATCCAGCTG CAATTACCAT ACTGGAAACT 
GCACAGAAAA TCGCAAAAGC TGAAGCAAAT TCAAGTGAGA AAGAGGAGGA TATTTTCAAG 
GGAATGGTAT GTCCACCTGG TACCAATGCA GACGACTCTC CTCTATATGT ACTTTGCTGC 
AATGATACCT GCAGTTTTAC ATGGACAGGA GCTGAGCATA TCAATCAGGA CATATTTGAA 
TGTCGAACAT GTGGCTTGTT GGAATCCCTG TGCTGCTGTA CCGAGTGTGC AAGAGTCTGC 
CACAAAGGCC ATGACTGCAA ATTGAAAAGA ACATCACCTA CTGCTTACTG CGACTGTTGG 
GAGAAATGCA AATGCAAAAC ATTAATTGCC GGGCAAAAGT CTGCACGACT TGATCTGCTC 
TATCGACTGC TGACCATCAC TAATCTTGTT ACAATGCCAA ATAGCAGGGG AGAGCACCTA 
CTTCTTTTCT TAGTGCAGAC TGTAGCCAGA CAGACAGTGG AGCACTGCCA GTACCGACCT 
CCAAGGATTC GAGAAGATCG CAATCGAAAG GCAGCTAACC CTGAAGATTC CGACATGCCA 
GATCATGACC TAGAGCCCCC TAGGTTTGCC CAGCTTGCAC TGGAACGTGT TTTACAAGAT 
TGGAACGCAC TCAAATCCAT GATTATGTTT GGATCCCAAG AAAATAAAGA CCCCCTTAGT 
GCTAGCAGTA GAATCGGGCA TCTTCTTCCA GAGGAACAAG TTTACTTGAA TCAGCAGAGT 
GGTGCCATTC GCTTGGACTG TTTTACTCAT TGCCTAATTG TCAAGTGCAC AGCAGATATT 
TTGCTGTTGG ATACCTTGCT GGGAACCCTT GTAAAAGAAC TCCAAAATAA ATATACCCCT 
GGGCGTAGAG AAGAAGCTGT AGCTGTCACA ATGCGTTTTC TGCGTTCAGT GGCTCGTGTT 
TTTGTCATTC TCAGTGTAGA AATGGCTTCA TCCAAGAAGA AAAACAATTT CATTCCACAG 
CCAATAGGGA AATGCAAGCG AGTGTTCCAA GCTTTACTTC CATATGCAGT GGAGGAGTTG 
TGTAATGTTG CAGAATCCCT CATTGTCCCA GTGAGAATGG GCATTGCACG CCCCACTGCC 
CCCTTCACAT TAGCAAGTAC AAGCATAGAT GCCATGCAGG GCAGCGAGGA GCTGTTCTCT 
GTGGAGCCTC TTCCTCCGAG ACCTTCATCT GATCAGTCTA ACAGGAATCC TCCGCAAAGA 
AGAATAAGCC AGTCCCAGCC TGTGCGAGGC CGAGATGAAG AGCAGGATGA CATTGTATCA 
GCAGATGTGG AAGAGGTTGA AGTGGTAGAG GGGGTAGCAG GAGAAGAGGA CCATCATGAT 
GAGCAAGAAG AGCATGGGGA GGAGAATACA GAAGCAGAAG GACAACATGA TGAGCACGAT 
GAAGATGGTA GTGACATGGA GTTAGACCTT CTTGCTGCAG CTGAAACAGA AAGTGACAGT 
GAAAGTAACC ACAGCAATCA GGATAATGCC AGCGGTCGGA GGAGTGTAGT AACAGCAGCT 
ACCGCTGGAT CAGAAGCAGG TGCCAGCAGC GTTCCGGCCT TTTTCTCTGA AGATGACTCC 
CAATCCAATG ACTCCAGTGA TTCTGATAGC AGCAGCAGTC AAAGTGATGA CCTGGACCAG 
GAGACCTTTA TGATTGATGA GCCTCTAGAG AGGTCGTCCA ACAGCAATCA TGCAAATGGG 
GCTGCCCAGG CTCCAAGGTC AATGCAGTGG GCAGTACGGA ACACTCAGAG CCAAAGAGCC 
CCAAACACCA CACCATCTAA TACTTCTACT CCAGCAGCCA GTTCTGGTGC CTTGATTTAC 
ATTGAACCAT CCAGTCTGAG GCGCACTGGC AACATAAGCT CCAGTGCAGC AGCAGCAGCC 
GCAGCCCTTG AAGCAAGCAA TTCTAGCAGT TATCTAACTT CAGCAAGCAG TCTGGCTAGA 
GCCTACAGCA TAGTGATACG GCAGATATCT GACTTGATGG GTCTAATCCC CAAGTACAAC 
CACTTGGTCT ATTCACAGAT CCCAGCAGCC GTCAAACTGA ACTATCAAGA TGCCGTTAAC 
TTACAGAACT ATGTTGAAGA AAAACTTATT CCCACATGGA ACTGGATGGT GAGCATAATG 
GACTCCACTG AAGCCCAGCT CCGTTATGGC TCCGCCTTAT CATCCGCTGG AGATCCAGGT 
CATCCCAACC ATCCTCTTCA TGCATCTCAG AATTCCAACC GCAGGGAAAG GATGACTGCC 
CGTGAGGAAG CTAGTTTGAG GACTCTTGAG GGAAGAAGAA GAGCCACTCT GCTTACTGCT 
CGACAGGGGA TGTCGGCCCG TGGAGACTTC CTGAATTATG CACTTTCACT AATGCGTTCG 
CACAATGATG AGCACTCTGA TGTACTCCCT GTTTTGGATG TTTGCTCCCT GAAACACGTG 
GCCTATGTCT TCCAGGCTCT GATATACTGG ATTAAGGCAA TGAACCAACA AACAACATTG 
GATACTCCAC AGCTGGAAAG AAAGAGGACA AGAGAGCTTT TGGAGCTTGG CCTTGATAAT 
GAAGATTCAG AGCATGAAAA TGATGATGAC ACAAATCAAA GCCTAATATT GAATGACAAA 
GACGACGATA CACTACCCTC TGAGACCGGT CCAAACCATC CGTTTTTCCG TCGTTCCGAT 
TCAATGACGT TCCTTGGTTG TATTCCACCA AACCCCTTTG AAGTTCCTCT GGCAGAAGCT 
ATCCCACTTG CAGACCAGCC CCATCTTTTG CAGCCAAATG CAAGGAAAGA AGACTTATTT 
GGTCGACCGA GTCAAGGTCT CTACTCATCT ACTGCTAACA CTGGAAAATG CCTATTAGAA 
GTGACAGTTG ACAGAAACTG CCTGGAGGTT TTACCAACCA AAATGTCATA TGCTGCTAAT 
CTAAAAAATG TAATGAACAT GCAGAGCCGT CAGAAAGAAG CAGAGGAACA GAATGTGCCG 
GCTGAGGAAA ATGACACATC AAAGGCTGGA CCCTCAGCTC ATGATCTTGC AGCTCAGCTG 
AAAAGTAGCT TGTTGGCAGA AATAGGGTTA ACAGAGAGTG AAGGTCCCCC GCTTACATCA 
TTCAGGCCAC AGTGTAGCTT TATGGGAATG GTCATTTCCC ATGATATGCT GTTAGGACGT 
TGGCGACTAT CTCTTGAATT GTTTGGAAGG GTGTTTATGG AAGATGTCGG AGCAGAGCCT 
GGATCGATCT TAACTGAACT AGGAGGGTTT GAAGTAAAGG AATCCAAGTT CCGCAGGGAG 
ATGGAAAAAC TTCGAAATCA GCAGTCCAGA GATCTTACAC TAGAGGTGGA TCGAGATAGA 
GATCTTCTAA TACAGCAAAC GATGAGGCAG CTGAATAATC ATTTTGGGCG CAGATGCGCC 
ACAACTCCTA TGGCAGTGCA TAGAGTAAAG GTCACCTTTA AAGATGAGCC AGGAGAAGGC 
AGTGGAGTAG CCCGAAGTTT TTATACTGCA GTAGCACAAG CATTTTTGTC CAATGAAAAG 
TTGCCAAGCT TGGACTGTAT CCAAAATGCC AACAAAGGTA CTCATACAAG TTTGATGCAA 
AGACTCAGAA ATCGTGGGGA ACGAGACCGG GAAAGGGAGC GGGACATGAG AAGAAGCAGT 
GGTTTGCGAA CAGGTTCTCG GCGGGATAGA GACAGAGATT TCAGACGGCA ACTTTCCATT 
GATACTAGGC CTTTCAGGCC AGCTTCTGAG GGCAATCCTA GTGATGATCC TGATCCTCTT 
CCTGCTCACC GACAGGCTCT CGGAGAAAGA CTATACCCAC GTGTACAAGC CATGCAGCCG 
GCATTTGCAA GTAAAATTAC AGGCATGCTG CTTGAGCTAT CTCCTGCACA ACTGCTGCTG 
CTGTTGGCAA GTGAAGACTC TCTAAGAGCC AGAGTGGATG AAGCTATGGA ACTTATTATT 
GCACATGGAA GGGAGAATGG TGCTGACAGT ATCCTCGATT TAGGTTTATT AGAAGCAGCT 
GATAAGGTAC AGGAAAACCG TAAGCGCCAT GGCTCTAGTC GAAGCGTGGT TGATATGGAG 
CTTACTGACA CAGACGATGG GGACGATAAT GCTCCTTTAT TTTACCAACC TGGTAAACGA 
GGTTTCTATA GTCCAAGGCC TGGAAAAAAC ACAGAGGCAC GTTTAAACTG TTTCCGCAAC 
ATCGGCAGAA TTCTTGGTCT GTGCCTACTA CAGAATGAAT TATGTCCTAT CACTTTAAGT 
AGGCAGGTGA TTAAAGTGCT TCTGGGCAGA AAGGTGAACT GGCATGATTT TGCGTTCTTT 
GATCACGTGA TGTACGAGAG CTTACGGCAA CTTATACTCG CCTCCCAAAG CCCGGATGCT 
GACGCAGTGT TTGCCTCAAT GGACCTAGCG TTTGCAATAG ACCTCTGCAA AGAAGAAGGT 
GGTGGACAAG TGGAACTTAT TCCTAATGGC GTAAATATCC CTGTAACCCC TCAAAATGTG 
TATGAATATG TAAGAAAATA TGCTGAACAC AGAATGCTCA CTGTAGCAGA GCAACCGCTT 
AATGCCATGA GGAAAGGACT ATTGGATGTT CTCCCAAAGA ATGCATTAGA AGACTTAACT 
GCAGAAGACT TTAGGCTCTT AGTGAATGGC TGTGGGGAAG TTAATGTTCA GATGCTCATT 
AGCTTTACCT CCTTCAACGA TGAGTCAGGA GAAAACGCAG AAAAGCTGTT GCAGTTCAAA 
CGCTGGTTCT GGTCAATCGT AGAGAAGATG AGTATGACAG AGCGGCAAGA TTTAGTTTAT 
TTTTGGACCT CAAGCCCATC ATTGCCTGCG AGTGAAGAGG GATTCCAGCC CATGCCATCC 
ATTACCATCC GGCCCCCCGA TGATCAGCAC CTTCCCACGG CGAACACCTG CATCTCTCGC 
CTCTACGTCC CCCTTTATTC TTCCAAACAG ATCCTGAAAC AGAAGTTGTT ACTTGCTATT 
AAGACCAAGA ACTTTGGATT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018079389.1
CDS	438..8804
Translation	MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLSSHPPLNVLEQTTIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELSKPENNDGSKLSSGSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGEDLMSLLDADIHSAHPSVIIDADAMFSEDISYFGYPSFRRSSLSRIGSSRVLLLPLERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPTLDKKNTPIMSPVSLGEDLQWWPEKDGTKFISIGALYSELVAVSSKGELYQWKWIEVEPYRNTQNPSLRHPRTSFLGLTNEKIVLLSANSIRATVATESNKVATWVDETLSSVASKLEHTAQIFPELQGDKIVSLHCCALYTCAQLENNLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNLEKTNKTIEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEERVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSNNSNNSNNSNNPCTPGSDPDPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGTWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLTNLPANSTIKKKAAIIIMAVEKQTLMQHILRGDYEACRQYLMNLEQGVVLDPNTQMLQTFLSHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTASTVPGQGPNTSTIPGPSSEPSVVESKDRKANAHFILKLLCDSIVLQPYLRDLLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEANSSEKEEDIFKGMVCPPGTNADDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTITNLVTMPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKAANPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGAIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAVAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSNRNPPQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENTEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDLDQETFMIDEPLERSSNSNHANGAAQAPRSMQWAVRNTQSQRAPNTTPSNTSTPAASSGALIYIEPSSLRRTGNISSSAAAAAAALEASNSSSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLNYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALSSAGDPGHPNHPLHASQNSNRRERMTAREEASLRTLEGRRRATLLTARQGMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGLDNEDSEHENDDDTNQSLILNDKDDDTLPSETGPNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSTANTGKCLLEVTVDRNCLEVLPTKMSYAANLKNVMNMQSRQKEAEEQNVPAEENDTSKAGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLTLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAVAQAFLSNEKLPSLDCIQNANKGTHTSLMQRLRNRGERDRERERDMRRSSGLRTGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPDPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLLEAADKVQENRKRHGSSRSVVDMELTDTDDGDDNAPLFYQPGKRGFYSPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLSRQVIKVLLGRKVNWHDFAFFDHVMYESLRQLILASQSPDADAVFASMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLTVAEQPLNAMRKGLLDVLPKNALEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV

Target ORF information:

RefSeq Version	XM_018223900.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018223900.1

ORF Insert Sequence:

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAAGA TCTTATGTCA CTGCTGGATG CTGACATTCA TTCTGCTCAC 
CCAAGTGTCA TAATTGATGC TGATGCTATG TTTTCAGAAG ATATTAGCTA CTTTGGTTAT 
CCATCTTTTC GGCGCTCTTC ACTTTCCAGG ATAGGCTCAT CCAGAGTTCT CCTTCTTCCC 
TTAGAGAGAG ACTCTGAGCT GTTGCGTGAA CGTGAGTCAG TGTTGCGTTT GCGTGAGCGG 
AGGTGGCTTG ATGGGGCCTC GTTTGATAAT GAAAGAGGCT CCACAAGTAA AGAAGGGGAA 
CCTACACTCG ATAAGAAGAA CACTCCCATT ATGAGCCCAG TGTCACTGGG AGAAGACCTG 
CAATGGTGGC CTGAAAAGGA TGGGACAAAG TTTATTTCTA TTGGAGCACT TTATTCCGAG 
CTGGTTGCTG TAAGCAGTAA GGGAGAACTT TATCAGTGGA AGTGGATCGA GGTGGAGCCT 
TATAGGAATA CACAGAATCC ATCATTACGT CATCCAAGAA CATCCTTTTT AGGCCTAACA 
AATGAGAAAA TAGTCCTGTT GTCAGCAAAC AGTATCCGAG CCACAGTCGC TACAGAAAGC 
AACAAGGTTG CCACTTGGGT CGATGAAACA TTAAGCTCGG TAGCCTCAAA GTTAGAACAC 
ACAGCTCAGA TTTTCCCTGA GCTACAAGGA GACAAGATTG TCTCGTTGCA CTGTTGTGCA 
CTTTATACTT GTGCGCAATT AGAAAATAAC CTTTATTGGT GGGGTGTGGT TCCTTTCAGT 
CAGAGGAAGA AAATGCTTGA AAAAGCTCGT GCAAAGAATA AAAAACCCAA ATCTAGTGCC 
GGAATTTCAT CCATGCCAAA TATCACAGTT GGCACCCAGG TATGCTTAAG AAATAACCCT 
CTCTACCATG CGGGAGCTGT TGCTTTCTCG ATCAGTGCTG GCATTCCCAA AGTAGGAGTA 
TTAATGGAAT CTGTGTGGAA TATGAATGAT AGCTGCAGAT TTCAGCTCCG TTCTCCAGAG 
AGTTTAAAAA ACTTGGAAAA AACAAATAAA ACAATTGAAG CCAAACCTGA GAGCAAACAG 
GAACCTGTAA AAACAGAGAT GGGCCCGCCC CCATCTCCCG CATCTACCTG TAGTGATGCC 
TCTTCAATTG CTAGCAGTGC CTCCATGCCA TACAAACGAA GACGATCAAC TCCTGCTCCT 
AAGGAAGAAG AGAGAGTGAA TGAAGAACAG TGGTCTCTCA GAGAAGTTGT TTTTGTAGAA 
GATGTTAAAA ATGTTCCTGT TGGGAAGGTA CTAAAAGTTG ATGGGGCCTA TGTTGCAGTC 
AAATTTCCTG GCACTTCTAA TAATTCTAAT AATTCTAATA ATTCTAACAA TCCGTGCACC 
CCTGGCTCTG ATCCAGACCC ATCCTCTCTC CTCCAGGATT GTAGATTACT ACGGATTGAT 
GAACTACAGG TAGTGAAAAC TGGGGGGACA CCCAAAGTCC CAGACTGTTT CCAAAGGACA 
CCTAAAAAGC TGTGCATTCC TGAAAAAACT GAGATCCTGG CAGTCAATGT AGATTCCAAA 
GGGGTTCACG CGGTACTGAA AACAGGGACA TGGGTACGGT ACTGTATCTT TGATCTTGCA 
ACTGGAAAAG CAGAACAGGA AAACAACTTC CCAACAAGTA GCATTGCATT TCTTGGCCAG 
AATGAGAGAA ATGTTGCTAT TTTTACAGCT GGGCAGGAAT CTCCCATCAT TCTTAGAGAT 
GGCAATGGTA CCATTTATCC CATGGCAAAA GATTGCATGG GTGGGATAAG AGATCCAGAC 
TGGCTTGACC TTCCTCCAAT AAGCAGTCTT GGAATGGGAG TTCATTCTTT AACCAATCTC 
CCTGCAAATT CAACAATTAA GAAGAAAGCA GCCATTATCA TAATGGCTGT TGAGAAGCAA 
ACACTGATGC AACACATTTT GCGCGGTGAT TATGAGGCCT GCAGGCAGTA TTTGATGAAC 
CTAGAGCAAG GTGTAGTTTT GGATCCAAAC ACTCAAATGC TGCAGACTTT TCTGAGCCAC 
AGATGTGATG GCAATAGGAA CATATTGCAT GCCTGCGTGT CCGTTTGCTT CCCAACAAGC 
AACAAGGAAA CAAAAGAAGA GGAGGAAGCA GAGCGCTCTG AAAGGAATAC ATTTGCAGAA 
AGACTGTCTG CAGTAGAAGC AATTGCCAAT GCTATATCTG TGGTTTCTAG CAATGGTCCC 
GGAAATCGTG CTGGATCGTC CAGCAGTAGA AGCTTGAGAT TGAGGGAAAT GATGAGGAGG 
TCCCTTCGGG CAGCAGGTTT AGGAAGACAT GAGGCTGGTG CTTCTAGTGA TCACCAAGAC 
CCAGTATCTC CTCCCATTGC ACCTCCTAGC TGGGTTCCTG ATCCACCTGC TATGGATCCA 
GATGGTGACA TAGATTTTAT CCTGGCCCCT GCTGTAGGAT CTCTTACCAC TGCATCCACT 
GTACCTGGCC AAGGACCAAA TACTTCTACA ATACCAGGTC CTTCCTCAGA GCCATCAGTT 
GTTGAGTCCA AGGATCGGAA GGCAAATGCT CACTTCATAC TGAAGTTATT ATGCGATAGC 
ATCGTTCTGC AGCCTTACCT ACGTGATCTC CTGTCTGCCA AGGATGCAAG AGGTATGACT 
CCCTTCATGT CTGCTGTTAG TGGAAGAGCC TATCCAGCTG CAATTACCAT ACTGGAAACT 
GCACAGAAAA TCGCAAAAGC TGAAGCAAAT TCAAGTGAGA AAGAGGAGGA TATTTTCAAG 
GGAATGGTAT GTCCACCTGG TACCAATGCA GACGACTCTC CTCTATATGT ACTTTGCTGC 
AATGATACCT GCAGTTTTAC ATGGACAGGA GCTGAGCATA TCAATCAGGA CATATTTGAA 
TGTCGAACAT GTGGCTTGTT GGAATCCCTG TGCTGCTGTA CCGAGTGTGC AAGAGTCTGC 
CACAAAGGCC ATGACTGCAA ATTGAAAAGA ACATCACCTA CTGCTTACTG CGACTGTTGG 
GAGAAATGCA AATGCAAAAC ATTAATTGCC GGGCAAAAGT CTGCACGACT TGATCTGCTC 
TATCGACTGC TGACCATCAC TAATCTTGTT ACAATGCCAA ATAGCAGGGG AGAGCACCTA 
CTTCTTTTCT TAGTGCAGAC TGTAGCCAGA CAGACAGTGG AGCACTGCCA GTACCGACCT 
CCAAGGATTC GAGAAGATCG CAATCGAAAG GCAGCTAACC CTGAAGATTC CGACATGCCA 
GATCATGACC TAGAGCCCCC TAGGTTTGCC CAGCTTGCAC TGGAACGTGT TTTACAAGAT 
TGGAACGCAC TCAAATCCAT GATTATGTTT GGATCCCAAG AAAATAAAGA CCCCCTTAGT 
GCTAGCAGTA GAATCGGGCA TCTTCTTCCA GAGGAACAAG TTTACTTGAA TCAGCAGAGT 
GGTGCCATTC GCTTGGACTG TTTTACTCAT TGCCTAATTG TCAAGTGCAC AGCAGATATT 
TTGCTGTTGG ATACCTTGCT GGGAACCCTT GTAAAAGAAC TCCAAAATAA ATATACCCCT 
GGGCGTAGAG AAGAAGCTGT AGCTGTCACA ATGCGTTTTC TGCGTTCAGT GGCTCGTGTT 
TTTGTCATTC TCAGTGTAGA AATGGCTTCA TCCAAGAAGA AAAACAATTT CATTCCACAG 
CCAATAGGGA AATGCAAGCG AGTGTTCCAA GCTTTACTTC CATATGCAGT GGAGGAGTTG 
TGTAATGTTG CAGAATCCCT CATTGTCCCA GTGAGAATGG GCATTGCACG CCCCACTGCC 
CCCTTCACAT TAGCAAGTAC AAGCATAGAT GCCATGCAGG GCAGCGAGGA GCTGTTCTCT 
GTGGAGCCTC TTCCTCCGAG ACCTTCATCT GATCAGTCTA ACAGGAATCC TCCGCAAAGA 
AGAATAAGCC AGTCCCAGCC TGTGCGAGGC CGAGATGAAG AGCAGGATGA CATTGTATCA 
GCAGATGTGG AAGAGGTTGA AGTGGTAGAG GGGGTAGCAG GAGAAGAGGA CCATCATGAT 
GAGCAAGAAG AGCATGGGGA GGAGAATACA GAAGCAGAAG GACAACATGA TGAGCACGAT 
GAAGATGGTA GTGACATGGA GTTAGACCTT CTTGCTGCAG CTGAAACAGA AAGTGACAGT 
GAAAGTAACC ACAGCAATCA GGATAATGCC AGCGGTCGGA GGAGTGTAGT AACAGCAGCT 
ACCGCTGGAT CAGAAGCAGG TGCCAGCAGC GTTCCGGCCT TTTTCTCTGA AGATGACTCC 
CAATCCAATG ACTCCAGTGA TTCTGATAGC AGCAGCAGTC AAAGTGATGA CCTGGACCAG 
GAGACCTTTA TGATTGATGA GCCTCTAGAG AGGTCGTCCA ACAGCAATCA TGCAAATGGG 
GCTGCCCAGG CTCCAAGGTC AATGCAGTGG GCAGTACGGA ACACTCAGAG CCAAAGAGCC 
CCAAACACCA CACCATCTAA TACTTCTACT CCAGCAGCCA GTTCTGGTGC CTTGATTTAC 
ATTGAACCAT CCAGTCTGAG GCGCACTGGC AACATAAGCT CCAGTGCAGC AGCAGCAGCC 
GCAGCCCTTG AAGCAAGCAA TTCTAGCAGT TATCTAACTT CAGCAAGCAG TCTGGCTAGA 
GCCTACAGCA TAGTGATACG GCAGATATCT GACTTGATGG GTCTAATCCC CAAGTACAAC 
CACTTGGTCT ATTCACAGAT CCCAGCAGCC GTCAAACTGA ACTATCAAGA TGCCGTTAAC 
TTACAGAACT ATGTTGAAGA AAAACTTATT CCCACATGGA ACTGGATGGT GAGCATAATG 
GACTCCACTG AAGCCCAGCT CCGTTATGGC TCCGCCTTAT CATCCGCTGG AGATCCAGGT 
CATCCCAACC ATCCTCTTCA TGCATCTCAG AATTCCAACC GCAGGGAAAG GATGACTGCC 
CGTGAGGAAG CTAGTTTGAG GACTCTTGAG GGAAGAAGAA GAGCCACTCT GCTTACTGCT 
CGACAGGGGA TGTCGGCCCG TGGAGACTTC CTGAATTATG CACTTTCACT AATGCGTTCG 
CACAATGATG AGCACTCTGA TGTACTCCCT GTTTTGGATG TTTGCTCCCT GAAACACGTG 
GCCTATGTCT TCCAGGCTCT GATATACTGG ATTAAGGCAA TGAACCAACA AACAACATTG 
GATACTCCAC AGCTGGAAAG AAAGAGGACA AGAGAGCTTT TGGAGCTTGG CCTTGATAAT 
GAAGATTCAG AGCATGAAAA TGATGATGAC ACAAATCAAA GCCTAATATT GAATGACAAA 
GACGACGATA CACTACCCTC TGAGACCGGT CCAAACCATC CGTTTTTCCG TCGTTCCGAT 
TCAATGACGT TCCTTGGTTG TATTCCACCA AACCCCTTTG AAGTTCCTCT GGCAGAAGCT 
ATCCCACTTG CAGACCAGCC CCATCTTTTG CAGCCAAATG CAAGGAAAGA AGACTTATTT 
GGTCGACCGA GTCAAGGTCT CTACTCATCT ACTGCTAACA CTGGAAAATG CCTATTAGAA 
GTGACAGTTG ACAGAAACTG CCTGGAGGTT TTACCAACCA AAATGTCATA TGCTGCTAAT 
CTAAAAAATG TAATGAACAT GCAGAGCCGT CAGAAAGAAG CAGAGGAACA GAATGTGCCG 
GCTGAGGAAA ATGACACATC AAAGGCTGGA CCCTCAGCTC ATGATCTTGC AGCTCAGCTG 
AAAAGTAGCT TGTTGGCAGA AATAGGGTTA ACAGAGAGTG AAGGTCCCCC GCTTACATCA 
TTCAGGCCAC AGTGTAGCTT TATGGGAATG GTCATTTCCC ATGATATGCT GTTAGGACGT 
TGGCGACTAT CTCTTGAATT GTTTGGAAGG GTGTTTATGG AAGATGTCGG AGCAGAGCCT 
GGATCGATCT TAACTGAACT AGGAGGGTTT GAAGTAAAGG AATCCAAGTT CCGCAGGGAG 
ATGGAAAAAC TTCGAAATCA GCAGTCCAGA GATCTTACAC TAGAGGTGGA TCGAGATAGA 
GATCTTCTAA TACAGCAAAC GATGAGGCAG CTGAATAATC ATTTTGGGCG CAGATGCGCC 
ACAACTCCTA TGGCAGTGCA TAGAGTAAAG GTCACCTTTA AAGATGAGCC AGGAGAAGGC 
AGTGGAGTAG CCCGAAGTTT TTATACTGCA GTAGCACAAG CATTTTTGTC CAATGAAAAG 
TTGCCAAGCT TGGACTGTAT CCAAAATGCC AACAAAGGTA CTCATACAAG TTTGATGCAA 
AGACTCAGAA ATCGTGGGGA ACGAGACCGG GAAAGGGAGC GGGACATGAG AAGAAGCAGT 
GGTTTGCGAA CAGGTTCTCG GCGGGATAGA GACAGAGATT TCAGACGGCA ACTTTCCATT 
GATACTAGGC CTTTCAGGCC AGCTTCTGAG GGCAATCCTA GTGATGATCC TGATCCTCTT 
CCTGCTCACC GACAGGCTCT CGGAGAAAGA CTATACCCAC GTGTACAAGC CATGCAGCCG 
GCATTTGCAA GTAAAATTAC AGGCATGCTG CTTGAGCTAT CTCCTGCACA ACTGCTGCTG 
CTGTTGGCAA GTGAAGACTC TCTAAGAGCC AGAGTGGATG AAGCTATGGA ACTTATTATT 
GCACATGGAA GGGAGAATGG TGCTGACAGT ATCCTCGATT TAGGTTTATT AGAAGCAGCT 
GATAAGGTAC AGGAAAACCG TAAGCGCCAT GGCTCTAGTC GAAGCGTGGT TGATATGGAG 
CTTACTGACA CAGACGATGG GGACGATAAT GCTCCTTTAT TTTACCAACC TGGTAAACGA 
GGTTTCTATA GTCCAAGGCC TGGAAAAAAC ACAGAGGCAC GTTTAAACTG TTTCCGCAAC 
ATCGGCAGAA TTCTTGGTCT GTGCCTACTA CAGAATGAAT TATGTCCTAT CACTTTAAGT 
AGGCAGGTGA TTAAAGTGCT TCTGGGCAGA AAGGTGAACT GGCATGATTT TGCGTTCTTT 
GATCACGTGA TGTACGAGAG CTTACGGCAA CTTATACTCG CCTCCCAAAG CCCGGATGCT 
GACGCAGTGT TTGCCTCAAT GGACCTAGCG TTTGCAATAG ACCTCTGCAA AGAAGAAGGT 
GGTGGACAAG TGGAACTTAT TCCTAATGGC GTAAATATCC CTGTAACCCC TCAAAATGTG 
TATGAATATG TAAGAAAATA TGCTGAACAC AGAATGCTCA CTGTAGCAGA GCAACCGCTT 
AATGCCATGA GGAAAGGACT ATTGGATGTT CTCCCAAAGA ATGCATTAGA AGACTTAACT 
GCAGAAGACT TTAGGCTCTT AGTGAATGGC TGTGGGGAAG TTAATGTTCA GATGCTCATT 
AGCTTTACCT CCTTCAACGA TGAGTCAGGA GAAAACGCAG AAAAGCTGTT GCAGTTCAAA 
CGCTGGTTCT GGTCAATCGT AGAGAAGATG AGTATGACAG AGCGGCAAGA TTTAGTTTAT 
TTTTGGACCT CAAGCCCATC ATTGCCTGCG AGTGAAGAGG GATTCCAGCC CATGCCATCC 
ATTACCATCC GGCCCCCCGA TGATCAGCAC CTTCCCACGG CGAACACCTG CATCTCTCGC 
CTCTACGTCC CCCTTTATTC TTCCAAACAG ATCCTGAAAC AGAAGTTGTT ACTTGCTATT 
AAGACCAAGA ACTTTGGATT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108695408 ( XM_018223901.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018079390.1 Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X7, mRNA.

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CloneID	OAf37369
Clone ID Related Accession (Same CDS sequence)	XM_018223901.1
Accession Version	XM_018223901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase UBR5-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGTTCT CCTTCTTCCC TTAGAGAGAG ACTCTGAGCT GTTGCGTGAA 
CGTGAGTCAG TGTTGCGTTT GCGTGAGCGG AGGTGGCTTG ATGGGGCCTC GTTTGATAAT 
GAAAGAGGCT CCACAAGTAA AGAAGGGGAA CCTACACTCG ATAAGAAGAA CACTCCCATT 
ATGAGCCCAG TGTCACTGGG AGAAGACCTG CAATGGTGGC CTGAAAAGGA TGGGACAAAG 
TTTATTTCTA TTGGAGCACT TTATTCCGAG CTGGTTGCTG TAAGCAGTAA GGGAGAACTT 
TATCAGTGGA AGTGGATCGA GGTGGAGCCT TATAGGAATA CACAGAATCC ATCATTACGT 
CATCCAAGAA CATCCTTTTT AGGCCTAACA AATGAGAAAA TAGTCCTGTT GTCAGCAAAC 
AGTATCCGAG CCACAGTCGC TACAGAAAGC AACAAGGTTG CCACTTGGGT CGATGAAACA 
TTAAGCTCGG TAGCCTCAAA GTTAGAACAC ACAGCTCAGA TTTTCCCTGA GCTACAAGGA 
GACAAGATTG TCTCGTTGCA CTGTTGTGCA CTTTATACTT GTGCGCAATT AGAAAATAAC 
CTTTATTGGT GGGGTGTGGT TCCTTTCAGT CAGAGGAAGA AAATGCTTGA AAAAGCTCGT 
GCAAAGAATA AAAAACCCAA ATCTAGTGCC GGAATTTCAT CCATGCCAAA TATCACAGTT 
GGCACCCAGG TATGCTTAAG AAATAACCCT CTCTACCATG CGGGAGCTGT TGCTTTCTCG 
ATCAGTGCTG GCATTCCCAA AGTAGGAGTA TTAATGGAAT CTGTGTGGAA TATGAATGAT 
AGCTGCAGAT TTCAGCTCCG TTCTCCAGAG AGTTTAAAAA ACTTGGAAAA AACAAATAAA 
ACAATTGAAG CCAAACCTGA GAGCAAACAG GAACCTGTAA AAACAGAGAT GGGCCCGCCC 
CCATCTCCCG CATCTACCTG TAGTGATGCC TCTTCAATTG CTAGCAGTGC CTCCATGCCA 
TACAAACGAA GACGATCAAC TCCTGCTCCT AAGGAAGAAG AGAGAGTGAA TGAAGAACAG 
TGGTCTCTCA GAGAAGTTGT TTTTGTAGAA GATGTTAAAA ATGTTCCTGT TGGGAAGGTA 
CTAAAAGTTG ATGGGGCCTA TGTTGCAGTC AAATTTCCTG GCACTTCTAA TAATTCTAAT 
AATTCTAATA ATTCTAACAA TCCGTGCACC CCTGGCTCTG ATCCAGACCC ATCCTCTCTC 
CTCCAGGATT GTAGATTACT ACGGATTGAT GAACTACAGG TAGTGAAAAC TGGGGGGACA 
CCCAAAGTCC CAGACTGTTT CCAAAGGACA CCTAAAAAGC TGTGCATTCC TGAAAAAACT 
GAGATCCTGG CAGTCAATGT AGATTCCAAA GGGGTTCACG CGGTACTGAA AACAGGGACA 
TGGGTACGGT ACTGTATCTT TGATCTTGCA ACTGGAAAAG CAGAACAGGA AAACAACTTC 
CCAACAAGTA GCATTGCATT TCTTGGCCAG AATGAGAGAA ATGTTGCTAT TTTTACAGCT 
GGGCAGGAAT CTCCCATCAT TCTTAGAGAT GGCAATGGTA CCATTTATCC CATGGCAAAA 
GATTGCATGG GTGGGATAAG AGATCCAGAC TGGCTTGACC TTCCTCCAAT AAGCAGTCTT 
GGAATGGGAG TTCATTCTTT AACCAATCTC CCTGCAAATT CAACAATTAA GAAGAAAGCA 
GCCATTATCA TAATGGCTGT TGAGAAGCAA ACACTGATGC AACACATTTT GCGCGGTGAT 
TATGAGGCCT GCAGGCAGTA TTTGATGAAC CTAGAGCAAG GTGTAGTTTT GGATCCAAAC 
ACTCAAATGC TGCAGACTTT TCTGAGCCAC AGATGTGATG GCAATAGGAA CATATTGCAT 
GCCTGCGTGT CCGTTTGCTT CCCAACAAGC AACAAGGAAA CAAAAGAAGA GGAGGAAGCA 
GAGCGCTCTG AAAGGAATAC ATTTGCAGAA AGACTGTCTG CAGTAGAAGC AATTGCCAAT 
GCTATATCTG TGGTTTCTAG CAATGGTCCC GGAAATCGTG CTGGATCGTC CAGCAGTAGA 
AGCTTGAGAT TGAGGGAAAT GATGAGGAGG TCCCTTCGGG CAGCAGGTTT AGGAAGACAT 
GAGGCTGGTG CTTCTAGTGA TCACCAAGAC CCAGTATCTC CTCCCATTGC ACCTCCTAGC 
TGGGTTCCTG ATCCACCTGC TATGGATCCA GATGGTGACA TAGATTTTAT CCTGGCCCCT 
GCTGTAGGAT CTCTTACCAC TGCATCCACT GTACCTGGCC AAGGACCAAA TACTTCTACA 
ATACCAGGTC CTTCCTCAGA GCCATCAGTT GTTGAGTCCA AGGATCGGAA GGCAAATGCT 
CACTTCATAC TGAAGTTATT ATGCGATAGC ATCGTTCTGC AGCCTTACCT ACGTGATCTC 
CTGTCTGCCA AGGATGCAAG AGGTATGACT CCCTTCATGT CTGCTGTTAG TGGAAGAGCC 
TATCCAGCTG CAATTACCAT ACTGGAAACT GCACAGAAAA TCGCAAAAGC TGAAGCAAAT 
TCAAGTGAGA AAGAGGAGGA TATTTTCAAG GGAATGGTAT GTCCACCTGG TACCAATGCA 
GACGACTCTC CTCTATATGT ACTTTGCTGC AATGATACCT GCAGTTTTAC ATGGACAGGA 
GCTGAGCATA TCAATCAGGA CATATTTGAA TGTCGAACAT GTGGCTTGTT GGAATCCCTG 
TGCTGCTGTA CCGAGTGTGC AAGAGTCTGC CACAAAGGCC ATGACTGCAA ATTGAAAAGA 
ACATCACCTA CTGCTTACTG CGACTGTTGG GAGAAATGCA AATGCAAAAC ATTAATTGCC 
GGGCAAAAGT CTGCACGACT TGATCTGCTC TATCGACTGC TGACCATCAC TAATCTTGTT 
ACAATGCCAA ATAGCAGGGG AGAGCACCTA CTTCTTTTCT TAGTGCAGAC TGTAGCCAGA 
CAGACAGTGG AGCACTGCCA GTACCGACCT CCAAGGATTC GAGAAGATCG CAATCGAAAG 
GCAGCTAACC CTGAAGATTC CGACATGCCA GATCATGACC TAGAGCCCCC TAGGTTTGCC 
CAGCTTGCAC TGGAACGTGT TTTACAAGAT TGGAACGCAC TCAAATCCAT GATTATGTTT 
GGATCCCAAG AAAATAAAGA CCCCCTTAGT GCTAGCAGTA GAATCGGGCA TCTTCTTCCA 
GAGGAACAAG TTTACTTGAA TCAGCAGAGT GGTGCCATTC GCTTGGACTG TTTTACTCAT 
TGCCTAATTG TCAAGTGCAC AGCAGATATT TTGCTGTTGG ATACCTTGCT GGGAACCCTT 
GTAAAAGAAC TCCAAAATAA ATATACCCCT GGGCGTAGAG AAGAAGCTGT AGCTGTCACA 
ATGCGTTTTC TGCGTTCAGT GGCTCGTGTT TTTGTCATTC TCAGTGTAGA AATGGCTTCA 
TCCAAGAAGA AAAACAATTT CATTCCACAG CCAATAGGGA AATGCAAGCG AGTGTTCCAA 
GCTTTACTTC CATATGCAGT GGAGGAGTTG TGTAATGTTG CAGAATCCCT CATTGTCCCA 
GTGAGAATGG GCATTGCACG CCCCACTGCC CCCTTCACAT TAGCAAGTAC AAGCATAGAT 
GCCATGCAGG GCAGCGAGGA GCTGTTCTCT GTGGAGCCTC TTCCTCCGAG ACCTTCATCT 
GATCAGTCTA ACAGTTCGAA TCAGTCCCAG TCATCCTATA TCATTAGGAA TCCTCCGCAA 
AGAAGAATAA GCCAGTCCCA GCCTGTGCGA GGCCGAGATG AAGAGCAGGA TGACATTGTA 
TCAGCAGATG TGGAAGAGGT TGAAGTGGTA GAGGGGGTAG CAGGAGAAGA GGACCATCAT 
GATGAGCAAG AAGAGCATGG GGAGGAGAAT ACAGAAGCAG AAGGACAACA TGATGAGCAC 
GATGAAGATG GTAGTGACAT GGAGTTAGAC CTTCTTGCTG CAGCTGAAAC AGAAAGTGAC 
AGTGAAAGTA ACCACAGCAA TCAGGATAAT GCCAGCGGTC GGAGGAGTGT AGTAACAGCA 
GCTACCGCTG GATCAGAAGC AGGTGCCAGC AGCGTTCCGG CCTTTTTCTC TGAAGATGAC 
TCCCAATCCA ATGACTCCAG TGATTCTGAT AGCAGCAGCA GTCAAAGTGA TGACCTGGAC 
CAGGAGACCT TTATGATTGA TGAGCCTCTA GAGAGGTCGT CCAACAGCAA TCATGCAAAT 
GGGGCTGCCC AGGCTCCAAG GTCAATGCAG TGGGCAGTAC GGAACACTCA GAGCCAAAGA 
GCCCCAAACA CCACACCATC TAATACTTCT ACTCCAGCAG CCAGTTCTGG TGCCTTGATT 
TACATTGAAC CATCCAGTCT GAGGCGCACT GGCAACATAA GCTCCAGTGC AGCAGCAGCA 
GCCGCAGCCC TTGAAGCAAG CAATTCTAGC AGTTATCTAA CTTCAGCAAG CAGTCTGGCT 
AGAGCCTACA GCATAGTGAT ACGGCAGATA TCTGACTTGA TGGGTCTAAT CCCCAAGTAC 
AACCACTTGG TCTATTCACA GATCCCAGCA GCCGTCAAAC TGAACTATCA AGATGCCGTT 
AACTTACAGA ACTATGTTGA AGAAAAACTT ATTCCCACAT GGAACTGGAT GGTGAGCATA 
ATGGACTCCA CTGAAGCCCA GCTCCGTTAT GGCTCCGCCT TATCATCCGC TGGAGATCCA 
GGTCATCCCA ACCATCCTCT TCATGCATCT CAGAATTCCA ACCGCAGGGA AAGGATGACT 
GCCCGTGAGG AAGCTAGTTT GAGGACTCTT GAGGGAAGAA GAAGAGCCAC TCTGCTTACT 
GCTCGACAGG GGATGTCGGC CCGTGGAGAC TTCCTGAATT ATGCACTTTC ACTAATGCGT 
TCGCACAATG ATGAGCACTC TGATGTACTC CCTGTTTTGG ATGTTTGCTC CCTGAAACAC 
GTGGCCTATG TCTTCCAGGC TCTGATATAC TGGATTAAGG CAATGAACCA ACAAACAACA 
TTGGATACTC CACAGCTGGA AAGAAAGAGG ACAAGAGAGC TTTTGGAGCT TGGCCTTGAT 
AATGAAGATT CAGAGCATGA AAATGATGAT GACACAAATC AAAGCCTAAT ATTGAATGAC 
AAAGACGACG ATACACTACC CTCTGAGACC GGTCCAAACC ATCCGTTTTT CCGTCGTTCC 
GATTCAATGA CGTTCCTTGG TTGTATTCCA CCAAACCCCT TTGAAGTTCC TCTGGCAGAA 
GCTATCCCAC TTGCAGACCA GCCCCATCTT TTGCAGCCAA ATGCAAGGAA AGAAGACTTA 
TTTGGTCGAC CGAGTCAAGG TCTCTACTCA TCTACTGCTA ACACTGGAAA ATGCCTATTA 
GAAGTGACAG TTGACAGAAA CTGCCTGGAG GTTTTACCAA CCAAAATGTC ATATGCTGCT 
AATCTAAAAA ATGTAATGAA CATGCAGAGC CGTCAGAAAG AAGCAGAGGA ACAGAATGTG 
CCGGCTGAGG AAAATGACAC ATCAAAGGCT GGACCCTCAG CTCATGATCT TGCAGCTCAG 
CTGAAAAGTA GCTTGTTGGC AGAAATAGGG TTAACAGAGA GTGAAGGTCC CCCGCTTACA 
TCATTCAGGC CACAGTGTAG CTTTATGGGA ATGGTCATTT CCCATGATAT GCTGTTAGGA 
CGTTGGCGAC TATCTCTTGA ATTGTTTGGA AGGGTGTTTA TGGAAGATGT CGGAGCAGAG 
CCTGGATCGA TCTTAACTGA ACTAGGAGGG TTTGAAGTAA AGGAATCCAA GTTCCGCAGG 
GAGATGGAAA AACTTCGAAA TCAGCAGTCC AGAGATCTTA CACTAGAGGT GGATCGAGAT 
AGAGATCTTC TAATACAGCA AACGATGAGG CAGCTGAATA ATCATTTTGG GCGCAGATGC 
GCCACAACTC CTATGGCAGT GCATAGAGTA AAGGTCACCT TTAAAGATGA GCCAGGAGAA 
GGCAGTGGAG TAGCCCGAAG TTTTTATACT GCAGTAGCAC AAGCATTTTT GTCCAATGAA 
AAGTTGCCAA GCTTGGACTG TATCCAAAAT GCCAACAAAG GTACTCATAC AAGTTTGATG 
CAAAGACTCA GAAATCGTGG GGAACGAGAC CGGGAAAGGG AGCGGGACAT GAGAAGAAGC 
AGTGGTTTGC GAACAGGTTC TCGGCGGGAT AGAGACAGAG ATTTCAGACG GCAACTTTCC 
ATTGATACTA GGCCTTTCAG GCCAGCTTCT GAGGGCAATC CTAGTGATGA TCCTGATCCT 
CTTCCTGCTC ACCGACAGGC TCTCGGAGAA AGACTATACC CACGTGTACA AGCCATGCAG 
CCGGCATTTG CAAGTAAAAT TACAGGCATG CTGCTTGAGC TATCTCCTGC ACAACTGCTG 
CTGCTGTTGG CAAGTGAAGA CTCTCTAAGA GCCAGAGTGG ATGAAGCTAT GGAACTTATT 
ATTGCACATG GAAGGGAGAA TGGTGCTGAC AGTATCCTCG ATTTAGGTTT ATTAGAAGCA 
GCTGATAAGG TACAGGAAAA CCGTAAGCGC CATGGCTCTA GTCGAAGCGT GGTTGATATG 
GAGCTTACTG ACACAGACGA TGGGGACGAT AATGCTCCTT TATTTTACCA ACCTGGTAAA 
CGAGGTTTCT ATAGTCCAAG GCCTGGAAAA AACACAGAGG CACGTTTAAA CTGTTTCCGC 
AACATCGGCA GAATTCTTGG TCTGTGCCTA CTACAGAATG AATTATGTCC TATCACTTTA 
AGTAGGCAGG TGATTAAAGT GCTTCTGGGC AGAAAGGTGA ACTGGCATGA TTTTGCGTTC 
TTTGATCACG TGATGTACGA GAGCTTACGG CAACTTATAC TCGCCTCCCA AAGCCCGGAT 
GCTGACGCAG TGTTTGCCTC AATGGACCTA GCGTTTGCAA TAGACCTCTG CAAAGAAGAA 
GGTGGTGGAC AAGTGGAACT TATTCCTAAT GGCGTAAATA TCCCTGTAAC CCCTCAAAAT 
GTGTATGAAT ATGTAAGAAA ATATGCTGAA CACAGAATGC TCACTGTAGC AGAGCAACCG 
CTTAATGCCA TGAGGAAAGG ACTATTGGAT GTTCTCCCAA AGAATGCATT AGAAGACTTA 
ACTGCAGAAG ACTTTAGGCT CTTAGTGAAT GGCTGTGGGG AAGTTAATGT TCAGATGCTC 
ATTAGCTTTA CCTCCTTCAA CGATGAGTCA GGAGAAAACG CAGAAAAGCT GTTGCAGTTC 
AAACGCTGGT TCTGGTCAAT CGTAGAGAAG ATGAGTATGA CAGAGCGGCA AGATTTAGTT 
TATTTTTGGA CCTCAAGCCC ATCATTGCCT GCGAGTGAAG AGGGATTCCA GCCCATGCCA 
TCCATTACCA TCCGGCCCCC CGATGATCAG CACCTTCCCA CGGCGAACAC CTGCATCTCT 
CGCCTCTACG TCCCCCTTTA TTCTTCCAAA CAGATCCTGA AACAGAAGTT GTTACTTGCT 
ATTAAGACCA AGAACTTTGG ATTTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018079390.1
CDS	438..8687
Translation	MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLSSHPPLNVLEQTTIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELSKPENNDGSKLSSGSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGVLLLPLERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPTLDKKNTPIMSPVSLGEDLQWWPEKDGTKFISIGALYSELVAVSSKGELYQWKWIEVEPYRNTQNPSLRHPRTSFLGLTNEKIVLLSANSIRATVATESNKVATWVDETLSSVASKLEHTAQIFPELQGDKIVSLHCCALYTCAQLENNLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNLEKTNKTIEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEERVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSNNSNNSNNSNNPCTPGSDPDPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGTWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLTNLPANSTIKKKAAIIIMAVEKQTLMQHILRGDYEACRQYLMNLEQGVVLDPNTQMLQTFLSHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTASTVPGQGPNTSTIPGPSSEPSVVESKDRKANAHFILKLLCDSIVLQPYLRDLLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEANSSEKEEDIFKGMVCPPGTNADDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTITNLVTMPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKAANPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGAIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAVAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSNSSNQSQSSYIIRNPPQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENTEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDLDQETFMIDEPLERSSNSNHANGAAQAPRSMQWAVRNTQSQRAPNTTPSNTSTPAASSGALIYIEPSSLRRTGNISSSAAAAAAALEASNSSSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLNYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALSSAGDPGHPNHPLHASQNSNRRERMTAREEASLRTLEGRRRATLLTARQGMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGLDNEDSEHENDDDTNQSLILNDKDDDTLPSETGPNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSTANTGKCLLEVTVDRNCLEVLPTKMSYAANLKNVMNMQSRQKEAEEQNVPAEENDTSKAGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLTLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAVAQAFLSNEKLPSLDCIQNANKGTHTSLMQRLRNRGERDRERERDMRRSSGLRTGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPDPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLLEAADKVQENRKRHGSSRSVVDMELTDTDDGDDNAPLFYQPGKRGFYSPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLSRQVIKVLLGRKVNWHDFAFFDHVMYESLRQLILASQSPDADAVFASMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLTVAEQPLNAMRKGLLDVLPKNALEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV

Target ORF information:

RefSeq Version	XM_018223901.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018223901.1

ORF Insert Sequence:

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGTTCT CCTTCTTCCC TTAGAGAGAG ACTCTGAGCT GTTGCGTGAA 
CGTGAGTCAG TGTTGCGTTT GCGTGAGCGG AGGTGGCTTG ATGGGGCCTC GTTTGATAAT 
GAAAGAGGCT CCACAAGTAA AGAAGGGGAA CCTACACTCG ATAAGAAGAA CACTCCCATT 
ATGAGCCCAG TGTCACTGGG AGAAGACCTG CAATGGTGGC CTGAAAAGGA TGGGACAAAG 
TTTATTTCTA TTGGAGCACT TTATTCCGAG CTGGTTGCTG TAAGCAGTAA GGGAGAACTT 
TATCAGTGGA AGTGGATCGA GGTGGAGCCT TATAGGAATA CACAGAATCC ATCATTACGT 
CATCCAAGAA CATCCTTTTT AGGCCTAACA AATGAGAAAA TAGTCCTGTT GTCAGCAAAC 
AGTATCCGAG CCACAGTCGC TACAGAAAGC AACAAGGTTG CCACTTGGGT CGATGAAACA 
TTAAGCTCGG TAGCCTCAAA GTTAGAACAC ACAGCTCAGA TTTTCCCTGA GCTACAAGGA 
GACAAGATTG TCTCGTTGCA CTGTTGTGCA CTTTATACTT GTGCGCAATT AGAAAATAAC 
CTTTATTGGT GGGGTGTGGT TCCTTTCAGT CAGAGGAAGA AAATGCTTGA AAAAGCTCGT 
GCAAAGAATA AAAAACCCAA ATCTAGTGCC GGAATTTCAT CCATGCCAAA TATCACAGTT 
GGCACCCAGG TATGCTTAAG AAATAACCCT CTCTACCATG CGGGAGCTGT TGCTTTCTCG 
ATCAGTGCTG GCATTCCCAA AGTAGGAGTA TTAATGGAAT CTGTGTGGAA TATGAATGAT 
AGCTGCAGAT TTCAGCTCCG TTCTCCAGAG AGTTTAAAAA ACTTGGAAAA AACAAATAAA 
ACAATTGAAG CCAAACCTGA GAGCAAACAG GAACCTGTAA AAACAGAGAT GGGCCCGCCC 
CCATCTCCCG CATCTACCTG TAGTGATGCC TCTTCAATTG CTAGCAGTGC CTCCATGCCA 
TACAAACGAA GACGATCAAC TCCTGCTCCT AAGGAAGAAG AGAGAGTGAA TGAAGAACAG 
TGGTCTCTCA GAGAAGTTGT TTTTGTAGAA GATGTTAAAA ATGTTCCTGT TGGGAAGGTA 
CTAAAAGTTG ATGGGGCCTA TGTTGCAGTC AAATTTCCTG GCACTTCTAA TAATTCTAAT 
AATTCTAATA ATTCTAACAA TCCGTGCACC CCTGGCTCTG ATCCAGACCC ATCCTCTCTC 
CTCCAGGATT GTAGATTACT ACGGATTGAT GAACTACAGG TAGTGAAAAC TGGGGGGACA 
CCCAAAGTCC CAGACTGTTT CCAAAGGACA CCTAAAAAGC TGTGCATTCC TGAAAAAACT 
GAGATCCTGG CAGTCAATGT AGATTCCAAA GGGGTTCACG CGGTACTGAA AACAGGGACA 
TGGGTACGGT ACTGTATCTT TGATCTTGCA ACTGGAAAAG CAGAACAGGA AAACAACTTC 
CCAACAAGTA GCATTGCATT TCTTGGCCAG AATGAGAGAA ATGTTGCTAT TTTTACAGCT 
GGGCAGGAAT CTCCCATCAT TCTTAGAGAT GGCAATGGTA CCATTTATCC CATGGCAAAA 
GATTGCATGG GTGGGATAAG AGATCCAGAC TGGCTTGACC TTCCTCCAAT AAGCAGTCTT 
GGAATGGGAG TTCATTCTTT AACCAATCTC CCTGCAAATT CAACAATTAA GAAGAAAGCA 
GCCATTATCA TAATGGCTGT TGAGAAGCAA ACACTGATGC AACACATTTT GCGCGGTGAT 
TATGAGGCCT GCAGGCAGTA TTTGATGAAC CTAGAGCAAG GTGTAGTTTT GGATCCAAAC 
ACTCAAATGC TGCAGACTTT TCTGAGCCAC AGATGTGATG GCAATAGGAA CATATTGCAT 
GCCTGCGTGT CCGTTTGCTT CCCAACAAGC AACAAGGAAA CAAAAGAAGA GGAGGAAGCA 
GAGCGCTCTG AAAGGAATAC ATTTGCAGAA AGACTGTCTG CAGTAGAAGC AATTGCCAAT 
GCTATATCTG TGGTTTCTAG CAATGGTCCC GGAAATCGTG CTGGATCGTC CAGCAGTAGA 
AGCTTGAGAT TGAGGGAAAT GATGAGGAGG TCCCTTCGGG CAGCAGGTTT AGGAAGACAT 
GAGGCTGGTG CTTCTAGTGA TCACCAAGAC CCAGTATCTC CTCCCATTGC ACCTCCTAGC 
TGGGTTCCTG ATCCACCTGC TATGGATCCA GATGGTGACA TAGATTTTAT CCTGGCCCCT 
GCTGTAGGAT CTCTTACCAC TGCATCCACT GTACCTGGCC AAGGACCAAA TACTTCTACA 
ATACCAGGTC CTTCCTCAGA GCCATCAGTT GTTGAGTCCA AGGATCGGAA GGCAAATGCT 
CACTTCATAC TGAAGTTATT ATGCGATAGC ATCGTTCTGC AGCCTTACCT ACGTGATCTC 
CTGTCTGCCA AGGATGCAAG AGGTATGACT CCCTTCATGT CTGCTGTTAG TGGAAGAGCC 
TATCCAGCTG CAATTACCAT ACTGGAAACT GCACAGAAAA TCGCAAAAGC TGAAGCAAAT 
TCAAGTGAGA AAGAGGAGGA TATTTTCAAG GGAATGGTAT GTCCACCTGG TACCAATGCA 
GACGACTCTC CTCTATATGT ACTTTGCTGC AATGATACCT GCAGTTTTAC ATGGACAGGA 
GCTGAGCATA TCAATCAGGA CATATTTGAA TGTCGAACAT GTGGCTTGTT GGAATCCCTG 
TGCTGCTGTA CCGAGTGTGC AAGAGTCTGC CACAAAGGCC ATGACTGCAA ATTGAAAAGA 
ACATCACCTA CTGCTTACTG CGACTGTTGG GAGAAATGCA AATGCAAAAC ATTAATTGCC 
GGGCAAAAGT CTGCACGACT TGATCTGCTC TATCGACTGC TGACCATCAC TAATCTTGTT 
ACAATGCCAA ATAGCAGGGG AGAGCACCTA CTTCTTTTCT TAGTGCAGAC TGTAGCCAGA 
CAGACAGTGG AGCACTGCCA GTACCGACCT CCAAGGATTC GAGAAGATCG CAATCGAAAG 
GCAGCTAACC CTGAAGATTC CGACATGCCA GATCATGACC TAGAGCCCCC TAGGTTTGCC 
CAGCTTGCAC TGGAACGTGT TTTACAAGAT TGGAACGCAC TCAAATCCAT GATTATGTTT 
GGATCCCAAG AAAATAAAGA CCCCCTTAGT GCTAGCAGTA GAATCGGGCA TCTTCTTCCA 
GAGGAACAAG TTTACTTGAA TCAGCAGAGT GGTGCCATTC GCTTGGACTG TTTTACTCAT 
TGCCTAATTG TCAAGTGCAC AGCAGATATT TTGCTGTTGG ATACCTTGCT GGGAACCCTT 
GTAAAAGAAC TCCAAAATAA ATATACCCCT GGGCGTAGAG AAGAAGCTGT AGCTGTCACA 
ATGCGTTTTC TGCGTTCAGT GGCTCGTGTT TTTGTCATTC TCAGTGTAGA AATGGCTTCA 
TCCAAGAAGA AAAACAATTT CATTCCACAG CCAATAGGGA AATGCAAGCG AGTGTTCCAA 
GCTTTACTTC CATATGCAGT GGAGGAGTTG TGTAATGTTG CAGAATCCCT CATTGTCCCA 
GTGAGAATGG GCATTGCACG CCCCACTGCC CCCTTCACAT TAGCAAGTAC AAGCATAGAT 
GCCATGCAGG GCAGCGAGGA GCTGTTCTCT GTGGAGCCTC TTCCTCCGAG ACCTTCATCT 
GATCAGTCTA ACAGTTCGAA TCAGTCCCAG TCATCCTATA TCATTAGGAA TCCTCCGCAA 
AGAAGAATAA GCCAGTCCCA GCCTGTGCGA GGCCGAGATG AAGAGCAGGA TGACATTGTA 
TCAGCAGATG TGGAAGAGGT TGAAGTGGTA GAGGGGGTAG CAGGAGAAGA GGACCATCAT 
GATGAGCAAG AAGAGCATGG GGAGGAGAAT ACAGAAGCAG AAGGACAACA TGATGAGCAC 
GATGAAGATG GTAGTGACAT GGAGTTAGAC CTTCTTGCTG CAGCTGAAAC AGAAAGTGAC 
AGTGAAAGTA ACCACAGCAA TCAGGATAAT GCCAGCGGTC GGAGGAGTGT AGTAACAGCA 
GCTACCGCTG GATCAGAAGC AGGTGCCAGC AGCGTTCCGG CCTTTTTCTC TGAAGATGAC 
TCCCAATCCA ATGACTCCAG TGATTCTGAT AGCAGCAGCA GTCAAAGTGA TGACCTGGAC 
CAGGAGACCT TTATGATTGA TGAGCCTCTA GAGAGGTCGT CCAACAGCAA TCATGCAAAT 
GGGGCTGCCC AGGCTCCAAG GTCAATGCAG TGGGCAGTAC GGAACACTCA GAGCCAAAGA 
GCCCCAAACA CCACACCATC TAATACTTCT ACTCCAGCAG CCAGTTCTGG TGCCTTGATT 
TACATTGAAC CATCCAGTCT GAGGCGCACT GGCAACATAA GCTCCAGTGC AGCAGCAGCA 
GCCGCAGCCC TTGAAGCAAG CAATTCTAGC AGTTATCTAA CTTCAGCAAG CAGTCTGGCT 
AGAGCCTACA GCATAGTGAT ACGGCAGATA TCTGACTTGA TGGGTCTAAT CCCCAAGTAC 
AACCACTTGG TCTATTCACA GATCCCAGCA GCCGTCAAAC TGAACTATCA AGATGCCGTT 
AACTTACAGA ACTATGTTGA AGAAAAACTT ATTCCCACAT GGAACTGGAT GGTGAGCATA 
ATGGACTCCA CTGAAGCCCA GCTCCGTTAT GGCTCCGCCT TATCATCCGC TGGAGATCCA 
GGTCATCCCA ACCATCCTCT TCATGCATCT CAGAATTCCA ACCGCAGGGA AAGGATGACT 
GCCCGTGAGG AAGCTAGTTT GAGGACTCTT GAGGGAAGAA GAAGAGCCAC TCTGCTTACT 
GCTCGACAGG GGATGTCGGC CCGTGGAGAC TTCCTGAATT ATGCACTTTC ACTAATGCGT 
TCGCACAATG ATGAGCACTC TGATGTACTC CCTGTTTTGG ATGTTTGCTC CCTGAAACAC 
GTGGCCTATG TCTTCCAGGC TCTGATATAC TGGATTAAGG CAATGAACCA ACAAACAACA 
TTGGATACTC CACAGCTGGA AAGAAAGAGG ACAAGAGAGC TTTTGGAGCT TGGCCTTGAT 
AATGAAGATT CAGAGCATGA AAATGATGAT GACACAAATC AAAGCCTAAT ATTGAATGAC 
AAAGACGACG ATACACTACC CTCTGAGACC GGTCCAAACC ATCCGTTTTT CCGTCGTTCC 
GATTCAATGA CGTTCCTTGG TTGTATTCCA CCAAACCCCT TTGAAGTTCC TCTGGCAGAA 
GCTATCCCAC TTGCAGACCA GCCCCATCTT TTGCAGCCAA ATGCAAGGAA AGAAGACTTA 
TTTGGTCGAC CGAGTCAAGG TCTCTACTCA TCTACTGCTA ACACTGGAAA ATGCCTATTA 
GAAGTGACAG TTGACAGAAA CTGCCTGGAG GTTTTACCAA CCAAAATGTC ATATGCTGCT 
AATCTAAAAA ATGTAATGAA CATGCAGAGC CGTCAGAAAG AAGCAGAGGA ACAGAATGTG 
CCGGCTGAGG AAAATGACAC ATCAAAGGCT GGACCCTCAG CTCATGATCT TGCAGCTCAG 
CTGAAAAGTA GCTTGTTGGC AGAAATAGGG TTAACAGAGA GTGAAGGTCC CCCGCTTACA 
TCATTCAGGC CACAGTGTAG CTTTATGGGA ATGGTCATTT CCCATGATAT GCTGTTAGGA 
CGTTGGCGAC TATCTCTTGA ATTGTTTGGA AGGGTGTTTA TGGAAGATGT CGGAGCAGAG 
CCTGGATCGA TCTTAACTGA ACTAGGAGGG TTTGAAGTAA AGGAATCCAA GTTCCGCAGG 
GAGATGGAAA AACTTCGAAA TCAGCAGTCC AGAGATCTTA CACTAGAGGT GGATCGAGAT 
AGAGATCTTC TAATACAGCA AACGATGAGG CAGCTGAATA ATCATTTTGG GCGCAGATGC 
GCCACAACTC CTATGGCAGT GCATAGAGTA AAGGTCACCT TTAAAGATGA GCCAGGAGAA 
GGCAGTGGAG TAGCCCGAAG TTTTTATACT GCAGTAGCAC AAGCATTTTT GTCCAATGAA 
AAGTTGCCAA GCTTGGACTG TATCCAAAAT GCCAACAAAG GTACTCATAC AAGTTTGATG 
CAAAGACTCA GAAATCGTGG GGAACGAGAC CGGGAAAGGG AGCGGGACAT GAGAAGAAGC 
AGTGGTTTGC GAACAGGTTC TCGGCGGGAT AGAGACAGAG ATTTCAGACG GCAACTTTCC 
ATTGATACTA GGCCTTTCAG GCCAGCTTCT GAGGGCAATC CTAGTGATGA TCCTGATCCT 
CTTCCTGCTC ACCGACAGGC TCTCGGAGAA AGACTATACC CACGTGTACA AGCCATGCAG 
CCGGCATTTG CAAGTAAAAT TACAGGCATG CTGCTTGAGC TATCTCCTGC ACAACTGCTG 
CTGCTGTTGG CAAGTGAAGA CTCTCTAAGA GCCAGAGTGG ATGAAGCTAT GGAACTTATT 
ATTGCACATG GAAGGGAGAA TGGTGCTGAC AGTATCCTCG ATTTAGGTTT ATTAGAAGCA 
GCTGATAAGG TACAGGAAAA CCGTAAGCGC CATGGCTCTA GTCGAAGCGT GGTTGATATG 
GAGCTTACTG ACACAGACGA TGGGGACGAT AATGCTCCTT TATTTTACCA ACCTGGTAAA 
CGAGGTTTCT ATAGTCCAAG GCCTGGAAAA AACACAGAGG CACGTTTAAA CTGTTTCCGC 
AACATCGGCA GAATTCTTGG TCTGTGCCTA CTACAGAATG AATTATGTCC TATCACTTTA 
AGTAGGCAGG TGATTAAAGT GCTTCTGGGC AGAAAGGTGA ACTGGCATGA TTTTGCGTTC 
TTTGATCACG TGATGTACGA GAGCTTACGG CAACTTATAC TCGCCTCCCA AAGCCCGGAT 
GCTGACGCAG TGTTTGCCTC AATGGACCTA GCGTTTGCAA TAGACCTCTG CAAAGAAGAA 
GGTGGTGGAC AAGTGGAACT TATTCCTAAT GGCGTAAATA TCCCTGTAAC CCCTCAAAAT 
GTGTATGAAT ATGTAAGAAA ATATGCTGAA CACAGAATGC TCACTGTAGC AGAGCAACCG 
CTTAATGCCA TGAGGAAAGG ACTATTGGAT GTTCTCCCAA AGAATGCATT AGAAGACTTA 
ACTGCAGAAG ACTTTAGGCT CTTAGTGAAT GGCTGTGGGG AAGTTAATGT TCAGATGCTC 
ATTAGCTTTA CCTCCTTCAA CGATGAGTCA GGAGAAAACG CAGAAAAGCT GTTGCAGTTC 
AAACGCTGGT TCTGGTCAAT CGTAGAGAAG ATGAGTATGA CAGAGCGGCA AGATTTAGTT 
TATTTTTGGA CCTCAAGCCC ATCATTGCCT GCGAGTGAAG AGGGATTCCA GCCCATGCCA 
TCCATTACCA TCCGGCCCCC CGATGATCAG CACCTTCCCA CGGCGAACAC CTGCATCTCT 
CGCCTCTACG TCCCCCTTTA TTCTTCCAAA CAGATCCTGA AACAGAAGTT GTTACTTGCT 
ATTAAGACCA AGAACTTTGG ATTTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108695408 ( XM_018223902.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018079391.1 Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X8, mRNA.

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CloneID	OAf37370
Clone ID Related Accession (Same CDS sequence)	XM_018223902.1
Accession Version	XM_018223902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase UBR5-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030735.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAGAG AGACTCTGAG CTGTTGCGTG AACGTGAGTC AGTGTTGCGT 
TTGCGTGAGC GGAGGTGGCT TGATGGGGCC TCGTTTGATA ATGAAAGAGG CTCCACAAGT 
AAAGAAGGGG AACCTACACT CGATAAGAAG AACACTCCCA TTATGAGCCC AGTGTCACTG 
GGAGAAGACC TGCAATGGTG GCCTGAAAAG GATGGGACAA AGTTTATTTC TATTGGAGCA 
CTTTATTCCG AGCTGGTTGC TGTAAGCAGT AAGGGAGAAC TTTATCAGTG GAAGTGGATC 
GAGGTGGAGC CTTATAGGAA TACACAGAAT CCATCATTAC GTCATCCAAG AACATCCTTT 
TTAGGCCTAA CAAATGAGAA AATAGTCCTG TTGTCAGCAA ACAGTATCCG AGCCACAGTC 
GCTACAGAAA GCAACAAGGT TGCCACTTGG GTCGATGAAA CATTAAGCTC GGTAGCCTCA 
AAGTTAGAAC ACACAGCTCA GATTTTCCCT GAGCTACAAG GAGACAAGAT TGTCTCGTTG 
CACTGTTGTG CACTTTATAC TTGTGCGCAA TTAGAAAATA ACCTTTATTG GTGGGGTGTG 
GTTCCTTTCA GTCAGAGGAA GAAAATGCTT GAAAAAGCTC GTGCAAAGAA TAAAAAACCC 
AAATCTAGTG CCGGAATTTC ATCCATGCCA AATATCACAG TTGGCACCCA GGTATGCTTA 
AGAAATAACC CTCTCTACCA TGCGGGAGCT GTTGCTTTCT CGATCAGTGC TGGCATTCCC 
AAAGTAGGAG TATTAATGGA ATCTGTGTGG AATATGAATG ATAGCTGCAG ATTTCAGCTC 
CGTTCTCCAG AGAGTTTAAA AAACTTGGAA AAAACAAATA AAACAATTGA AGCCAAACCT 
GAGAGCAAAC AGGAACCTGT AAAAACAGAG ATGGGCCCGC CCCCATCTCC CGCATCTACC 
TGTAGTGATG CCTCTTCAAT TGCTAGCAGT GCCTCCATGC CATACAAACG AAGACGATCA 
ACTCCTGCTC CTAAGGAAGA AGAGAGAGTG AATGAAGAAC AGTGGTCTCT CAGAGAAGTT 
GTTTTTGTAG AAGATGTTAA AAATGTTCCT GTTGGGAAGG TACTAAAAGT TGATGGGGCC 
TATGTTGCAG TCAAATTTCC TGGCACTTCT AATAATTCTA ATAATTCTAA TAATTCTAAC 
AATCCGTGCA CCCCTGGCTC TGATCCAGAC CCATCCTCTC TCCTCCAGGA TTGTAGATTA 
CTACGGATTG ATGAACTACA GGTAGTGAAA ACTGGGGGGA CACCCAAAGT CCCAGACTGT 
TTCCAAAGGA CACCTAAAAA GCTGTGCATT CCTGAAAAAA CTGAGATCCT GGCAGTCAAT 
GTAGATTCCA AAGGGGTTCA CGCGGTACTG AAAACAGGGA CATGGGTACG GTACTGTATC 
TTTGATCTTG CAACTGGAAA AGCAGAACAG GAAAACAACT TCCCAACAAG TAGCATTGCA 
TTTCTTGGCC AGAATGAGAG AAATGTTGCT ATTTTTACAG CTGGGCAGGA ATCTCCCATC 
ATTCTTAGAG ATGGCAATGG TACCATTTAT CCCATGGCAA AAGATTGCAT GGGTGGGATA 
AGAGATCCAG ACTGGCTTGA CCTTCCTCCA ATAAGCAGTC TTGGAATGGG AGTTCATTCT 
TTAACCAATC TCCCTGCAAA TTCAACAATT AAGAAGAAAG CAGCCATTAT CATAATGGCT 
GTTGAGAAGC AAACACTGAT GCAACACATT TTGCGCGGTG ATTATGAGGC CTGCAGGCAG 
TATTTGATGA ACCTAGAGCA AGGTGTAGTT TTGGATCCAA ACACTCAAAT GCTGCAGACT 
TTTCTGAGCC ACAGATGTGA TGGCAATAGG AACATATTGC ATGCCTGCGT GTCCGTTTGC 
TTCCCAACAA GCAACAAGGA AACAAAAGAA GAGGAGGAAG CAGAGCGCTC TGAAAGGAAT 
ACATTTGCAG AAAGACTGTC TGCAGTAGAA GCAATTGCCA ATGCTATATC TGTGGTTTCT 
AGCAATGGTC CCGGAAATCG TGCTGGATCG TCCAGCAGTA GAAGCTTGAG ATTGAGGGAA 
ATGATGAGGA GGTCCCTTCG GGCAGCAGGT TTAGGAAGAC ATGAGGCTGG TGCTTCTAGT 
GATCACCAAG ACCCAGTATC TCCTCCCATT GCACCTCCTA GCTGGGTTCC TGATCCACCT 
GCTATGGATC CAGATGGTGA CATAGATTTT ATCCTGGCCC CTGCTGTAGG ATCTCTTACC 
ACTGCATCCA CTGTACCTGG CCAAGGACCA AATACTTCTA CAATACCAGG TCCTTCCTCA 
GAGCCATCAG TTGTTGAGTC CAAGGATCGG AAGGCAAATG CTCACTTCAT ACTGAAGTTA 
TTATGCGATA GCATCGTTCT GCAGCCTTAC CTACGTGATC TCCTGTCTGC CAAGGATGCA 
AGAGGTATGA CTCCCTTCAT GTCTGCTGTT AGTGGAAGAG CCTATCCAGC TGCAATTACC 
ATACTGGAAA CTGCACAGAA AATCGCAAAA GCTGAAGCAA ATTCAAGTGA GAAAGAGGAG 
GATATTTTCA AGGGAATGGT ATGTCCACCT GGTACCAATG CAGACGACTC TCCTCTATAT 
GTACTTTGCT GCAATGATAC CTGCAGTTTT ACATGGACAG GAGCTGAGCA TATCAATCAG 
GACATATTTG AATGTCGAAC ATGTGGCTTG TTGGAATCCC TGTGCTGCTG TACCGAGTGT 
GCAAGAGTCT GCCACAAAGG CCATGACTGC AAATTGAAAA GAACATCACC TACTGCTTAC 
TGCGACTGTT GGGAGAAATG CAAATGCAAA ACATTAATTG CCGGGCAAAA GTCTGCACGA 
CTTGATCTGC TCTATCGACT GCTGACCATC ACTAATCTTG TTACAATGCC AAATAGCAGG 
GGAGAGCACC TACTTCTTTT CTTAGTGCAG ACTGTAGCCA GACAGACAGT GGAGCACTGC 
CAGTACCGAC CTCCAAGGAT TCGAGAAGAT CGCAATCGAA AGGCAGCTAA CCCTGAAGAT 
TCCGACATGC CAGATCATGA CCTAGAGCCC CCTAGGTTTG CCCAGCTTGC ACTGGAACGT 
GTTTTACAAG ATTGGAACGC ACTCAAATCC ATGATTATGT TTGGATCCCA AGAAAATAAA 
GACCCCCTTA GTGCTAGCAG TAGAATCGGG CATCTTCTTC CAGAGGAACA AGTTTACTTG 
AATCAGCAGA GTGGTGCCAT TCGCTTGGAC TGTTTTACTC ATTGCCTAAT TGTCAAGTGC 
ACAGCAGATA TTTTGCTGTT GGATACCTTG CTGGGAACCC TTGTAAAAGA ACTCCAAAAT 
AAATATACCC CTGGGCGTAG AGAAGAAGCT GTAGCTGTCA CAATGCGTTT TCTGCGTTCA 
GTGGCTCGTG TTTTTGTCAT TCTCAGTGTA GAAATGGCTT CATCCAAGAA GAAAAACAAT 
TTCATTCCAC AGCCAATAGG GAAATGCAAG CGAGTGTTCC AAGCTTTACT TCCATATGCA 
GTGGAGGAGT TGTGTAATGT TGCAGAATCC CTCATTGTCC CAGTGAGAAT GGGCATTGCA 
CGCCCCACTG CCCCCTTCAC ATTAGCAAGT ACAAGCATAG ATGCCATGCA GGGCAGCGAG 
GAGCTGTTCT CTGTGGAGCC TCTTCCTCCG AGACCTTCAT CTGATCAGTC TAACAGTTCG 
AATCAGTCCC AGTCATCCTA TATCATTAGG AATCCTCCGC AAAGAAGAAT AAGCCAGTCC 
CAGCCTGTGC GAGGCCGAGA TGAAGAGCAG GATGACATTG TATCAGCAGA TGTGGAAGAG 
GTTGAAGTGG TAGAGGGGGT AGCAGGAGAA GAGGACCATC ATGATGAGCA AGAAGAGCAT 
GGGGAGGAGA ATACAGAAGC AGAAGGACAA CATGATGAGC ACGATGAAGA TGGTAGTGAC 
ATGGAGTTAG ACCTTCTTGC TGCAGCTGAA ACAGAAAGTG ACAGTGAAAG TAACCACAGC 
AATCAGGATA ATGCCAGCGG TCGGAGGAGT GTAGTAACAG CAGCTACCGC TGGATCAGAA 
GCAGGTGCCA GCAGCGTTCC GGCCTTTTTC TCTGAAGATG ACTCCCAATC CAATGACTCC 
AGTGATTCTG ATAGCAGCAG CAGTCAAAGT GATGACCTGG ACCAGGAGAC CTTTATGATT 
GATGAGCCTC TAGAGAGGTC GTCCAACAGC AATCATGCAA ATGGGGCTGC CCAGGCTCCA 
AGGTCAATGC AGTGGGCAGT ACGGAACACT CAGAGCCAAA GAGCCCCAAA CACCACACCA 
TCTAATACTT CTACTCCAGC AGCCAGTTCT GGTGCCTTGA TTTACATTGA ACCATCCAGT 
CTGAGGCGCA CTGGCAACAT AAGCTCCAGT GCAGCAGCAG CAGCCGCAGC CCTTGAAGCA 
AGCAATTCTA GCAGTTATCT AACTTCAGCA AGCAGTCTGG CTAGAGCCTA CAGCATAGTG 
ATACGGCAGA TATCTGACTT GATGGGTCTA ATCCCCAAGT ACAACCACTT GGTCTATTCA 
CAGATCCCAG CAGCCGTCAA ACTGAACTAT CAAGATGCCG TTAACTTACA GAACTATGTT 
GAAGAAAAAC TTATTCCCAC ATGGAACTGG ATGGTGAGCA TAATGGACTC CACTGAAGCC 
CAGCTCCGTT ATGGCTCCGC CTTATCATCC GCTGGAGATC CAGGTCATCC CAACCATCCT 
CTTCATGCAT CTCAGAATTC CAACCGCAGG GAAAGGATGA CTGCCCGTGA GGAAGCTAGT 
TTGAGGACTC TTGAGGGAAG AAGAAGAGCC ACTCTGCTTA CTGCTCGACA GGGGATGTCG 
GCCCGTGGAG ACTTCCTGAA TTATGCACTT TCACTAATGC GTTCGCACAA TGATGAGCAC 
TCTGATGTAC TCCCTGTTTT GGATGTTTGC TCCCTGAAAC ACGTGGCCTA TGTCTTCCAG 
GCTCTGATAT ACTGGATTAA GGCAATGAAC CAACAAACAA CATTGGATAC TCCACAGCTG 
GAAAGAAAGA GGACAAGAGA GCTTTTGGAG CTTGGCCTTG ATAATGAAGA TTCAGAGCAT 
GAAAATGATG ATGACACAAA TCAAAGCCTA ATATTGAATG ACAAAGACGA CGATACACTA 
CCCTCTGAGA CCGGTCCAAA CCATCCGTTT TTCCGTCGTT CCGATTCAAT GACGTTCCTT 
GGTTGTATTC CACCAAACCC CTTTGAAGTT CCTCTGGCAG AAGCTATCCC ACTTGCAGAC 
CAGCCCCATC TTTTGCAGCC AAATGCAAGG AAAGAAGACT TATTTGGTCG ACCGAGTCAA 
GGTCTCTACT CATCTACTGC TAACACTGGA AAATGCCTAT TAGAAGTGAC AGTTGACAGA 
AACTGCCTGG AGGTTTTACC AACCAAAATG TCATATGCTG CTAATCTAAA AAATGTAATG 
AACATGCAGA GCCGTCAGAA AGAAGCAGAG GAACAGAATG TGCCGGCTGA GGAAAATGAC 
ACATCAAAGG CTGGACCCTC AGCTCATGAT CTTGCAGCTC AGCTGAAAAG TAGCTTGTTG 
GCAGAAATAG GGTTAACAGA GAGTGAAGGT CCCCCGCTTA CATCATTCAG GCCACAGTGT 
AGCTTTATGG GAATGGTCAT TTCCCATGAT ATGCTGTTAG GACGTTGGCG ACTATCTCTT 
GAATTGTTTG GAAGGGTGTT TATGGAAGAT GTCGGAGCAG AGCCTGGATC GATCTTAACT 
GAACTAGGAG GGTTTGAAGT AAAGGAATCC AAGTTCCGCA GGGAGATGGA AAAACTTCGA 
AATCAGCAGT CCAGAGATCT TACACTAGAG GTGGATCGAG ATAGAGATCT TCTAATACAG 
CAAACGATGA GGCAGCTGAA TAATCATTTT GGGCGCAGAT GCGCCACAAC TCCTATGGCA 
GTGCATAGAG TAAAGGTCAC CTTTAAAGAT GAGCCAGGAG AAGGCAGTGG AGTAGCCCGA 
AGTTTTTATA CTGCAGTAGC ACAAGCATTT TTGTCCAATG AAAAGTTGCC AAGCTTGGAC 
TGTATCCAAA ATGCCAACAA AGGTACTCAT ACAAGTTTGA TGCAAAGACT CAGAAATCGT 
GGGGAACGAG ACCGGGAAAG GGAGCGGGAC ATGAGAAGAA GCAGTGGTTT GCGAACAGGT 
TCTCGGCGGG ATAGAGACAG AGATTTCAGA CGGCAACTTT CCATTGATAC TAGGCCTTTC 
AGGCCAGCTT CTGAGGGCAA TCCTAGTGAT GATCCTGATC CTCTTCCTGC TCACCGACAG 
GCTCTCGGAG AAAGACTATA CCCACGTGTA CAAGCCATGC AGCCGGCATT TGCAAGTAAA 
ATTACAGGCA TGCTGCTTGA GCTATCTCCT GCACAACTGC TGCTGCTGTT GGCAAGTGAA 
GACTCTCTAA GAGCCAGAGT GGATGAAGCT ATGGAACTTA TTATTGCACA TGGAAGGGAG 
AATGGTGCTG ACAGTATCCT CGATTTAGGT TTATTAGAAG CAGCTGATAA GGTACAGGAA 
AACCGTAAGC GCCATGGCTC TAGTCGAAGC GTGGTTGATA TGGAGCTTAC TGACACAGAC 
GATGGGGACG ATAATGCTCC TTTATTTTAC CAACCTGGTA AACGAGGTTT CTATAGTCCA 
AGGCCTGGAA AAAACACAGA GGCACGTTTA AACTGTTTCC GCAACATCGG CAGAATTCTT 
GGTCTGTGCC TACTACAGAA TGAATTATGT CCTATCACTT TAAGTAGGCA GGTGATTAAA 
GTGCTTCTGG GCAGAAAGGT GAACTGGCAT GATTTTGCGT TCTTTGATCA CGTGATGTAC 
GAGAGCTTAC GGCAACTTAT ACTCGCCTCC CAAAGCCCGG ATGCTGACGC AGTGTTTGCC 
TCAATGGACC TAGCGTTTGC AATAGACCTC TGCAAAGAAG AAGGTGGTGG ACAAGTGGAA 
CTTATTCCTA ATGGCGTAAA TATCCCTGTA ACCCCTCAAA ATGTGTATGA ATATGTAAGA 
AAATATGCTG AACACAGAAT GCTCACTGTA GCAGAGCAAC CGCTTAATGC CATGAGGAAA 
GGACTATTGG ATGTTCTCCC AAAGAATGCA TTAGAAGACT TAACTGCAGA AGACTTTAGG 
CTCTTAGTGA ATGGCTGTGG GGAAGTTAAT GTTCAGATGC TCATTAGCTT TACCTCCTTC 
AACGATGAGT CAGGAGAAAA CGCAGAAAAG CTGTTGCAGT TCAAACGCTG GTTCTGGTCA 
ATCGTAGAGA AGATGAGTAT GACAGAGCGG CAAGATTTAG TTTATTTTTG GACCTCAAGC 
CCATCATTGC CTGCGAGTGA AGAGGGATTC CAGCCCATGC CATCCATTAC CATCCGGCCC 
CCCGATGATC AGCACCTTCC CACGGCGAAC ACCTGCATCT CTCGCCTCTA CGTCCCCCTT 
TATTCTTCCA AACAGATCCT GAAACAGAAG TTGTTACTTG CTATTAAGAC CAAGAACTTT 
GGATTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018079391.1
CDS	438..8669
Translation	MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLSSHPPLNVLEQTTIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELSKPENNDGSKLSSGSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPTLDKKNTPIMSPVSLGEDLQWWPEKDGTKFISIGALYSELVAVSSKGELYQWKWIEVEPYRNTQNPSLRHPRTSFLGLTNEKIVLLSANSIRATVATESNKVATWVDETLSSVASKLEHTAQIFPELQGDKIVSLHCCALYTCAQLENNLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNLEKTNKTIEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEERVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSNNSNNSNNSNNPCTPGSDPDPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGTWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLTNLPANSTIKKKAAIIIMAVEKQTLMQHILRGDYEACRQYLMNLEQGVVLDPNTQMLQTFLSHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTASTVPGQGPNTSTIPGPSSEPSVVESKDRKANAHFILKLLCDSIVLQPYLRDLLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEANSSEKEEDIFKGMVCPPGTNADDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTITNLVTMPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKAANPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGAIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAVAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSNSSNQSQSSYIIRNPPQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENTEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDLDQETFMIDEPLERSSNSNHANGAAQAPRSMQWAVRNTQSQRAPNTTPSNTSTPAASSGALIYIEPSSLRRTGNISSSAAAAAAALEASNSSSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLNYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALSSAGDPGHPNHPLHASQNSNRRERMTAREEASLRTLEGRRRATLLTARQGMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGLDNEDSEHENDDDTNQSLILNDKDDDTLPSETGPNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSTANTGKCLLEVTVDRNCLEVLPTKMSYAANLKNVMNMQSRQKEAEEQNVPAEENDTSKAGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLTLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAVAQAFLSNEKLPSLDCIQNANKGTHTSLMQRLRNRGERDRERERDMRRSSGLRTGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPDPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLLEAADKVQENRKRHGSSRSVVDMELTDTDDGDDNAPLFYQPGKRGFYSPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLSRQVIKVLLGRKVNWHDFAFFDHVMYESLRQLILASQSPDADAVFASMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLTVAEQPLNAMRKGLLDVLPKNALEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV

Target ORF information:

RefSeq Version	XM_018223902.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin-protein ligase UBR5-like (LOC108695408), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018223902.1

ORF Insert Sequence:

ATGACTTCCA TACACTTTGT GGTGCACCCG CTGCCCGGCA CGGAGGATCA GCTCAATGAT 
CGGCTAAGAG AAGTTTCCGA GAAGCTGAAC AAATACAACT TAAGCAGCCA CCCCCCTCTG 
AACGTACTTG AACAGACCAC TATAAAACAG TGTGTGGTTG GACCCAATCA TGCTGCTTTC 
CTTTTAGAGG ATGGAAGAGT TTGCAGAATT GGTTTCTCCG TGCAGCCAGA CAGACTGGAA 
TTAAGCAAAC CTGAAAATAA TGATGGTTCA AAGTTGAGCA GTGGCTCAGG GGCAGGAAGA 
ACATCAAGGC CAGGCAGGAC TAGTGACTCC CCGTGGTTTC TGTCTGGTTC TGAAACTCTG 
GGCAGACTGG CAGGCAACAC CCTTGGAAGC AGGTGGAGTT CTGGTGTTGG TGGAAGTGGT 
GGGGGATCCT CTGGGCGGTC ATCAGCTGGT GCTAGAGACT CTCGGAGGCA AACAAGAGTT 
ATACGCACAG GAAGGGACAG GGGCTCTGGT CTCCTGGGCA GTCAACCACA GCCTGTGATT 
CCAGCTTCTG TTATTCCTGA GGAGCTCATC TCACAGGCAC AAGTTGTTCT TCAAGGAAAA 
TCACGAAGTG TTATCATTCG AGAGCTGCAG AGAACAAATC TTGATGTGAA CCTTGCAGTA 
AACAATCTCT TGAGTCGAGA TGATGAAGAT GGGGATGATG GTGATGACAC TGCTAGTGAA 
TCTTACTTGC CTGGAGAGAG AGACTCTGAG CTGTTGCGTG AACGTGAGTC AGTGTTGCGT 
TTGCGTGAGC GGAGGTGGCT TGATGGGGCC TCGTTTGATA ATGAAAGAGG CTCCACAAGT 
AAAGAAGGGG AACCTACACT CGATAAGAAG AACACTCCCA TTATGAGCCC AGTGTCACTG 
GGAGAAGACC TGCAATGGTG GCCTGAAAAG GATGGGACAA AGTTTATTTC TATTGGAGCA 
CTTTATTCCG AGCTGGTTGC TGTAAGCAGT AAGGGAGAAC TTTATCAGTG GAAGTGGATC 
GAGGTGGAGC CTTATAGGAA TACACAGAAT CCATCATTAC GTCATCCAAG AACATCCTTT 
TTAGGCCTAA CAAATGAGAA AATAGTCCTG TTGTCAGCAA ACAGTATCCG AGCCACAGTC 
GCTACAGAAA GCAACAAGGT TGCCACTTGG GTCGATGAAA CATTAAGCTC GGTAGCCTCA 
AAGTTAGAAC ACACAGCTCA GATTTTCCCT GAGCTACAAG GAGACAAGAT TGTCTCGTTG 
CACTGTTGTG CACTTTATAC TTGTGCGCAA TTAGAAAATA ACCTTTATTG GTGGGGTGTG 
GTTCCTTTCA GTCAGAGGAA GAAAATGCTT GAAAAAGCTC GTGCAAAGAA TAAAAAACCC 
AAATCTAGTG CCGGAATTTC ATCCATGCCA AATATCACAG TTGGCACCCA GGTATGCTTA 
AGAAATAACC CTCTCTACCA TGCGGGAGCT GTTGCTTTCT CGATCAGTGC TGGCATTCCC 
AAAGTAGGAG TATTAATGGA ATCTGTGTGG AATATGAATG ATAGCTGCAG ATTTCAGCTC 
CGTTCTCCAG AGAGTTTAAA AAACTTGGAA AAAACAAATA AAACAATTGA AGCCAAACCT 
GAGAGCAAAC AGGAACCTGT AAAAACAGAG ATGGGCCCGC CCCCATCTCC CGCATCTACC 
TGTAGTGATG CCTCTTCAAT TGCTAGCAGT GCCTCCATGC CATACAAACG AAGACGATCA 
ACTCCTGCTC CTAAGGAAGA AGAGAGAGTG AATGAAGAAC AGTGGTCTCT CAGAGAAGTT 
GTTTTTGTAG AAGATGTTAA AAATGTTCCT GTTGGGAAGG TACTAAAAGT TGATGGGGCC 
TATGTTGCAG TCAAATTTCC TGGCACTTCT AATAATTCTA ATAATTCTAA TAATTCTAAC 
AATCCGTGCA CCCCTGGCTC TGATCCAGAC CCATCCTCTC TCCTCCAGGA TTGTAGATTA 
CTACGGATTG ATGAACTACA GGTAGTGAAA ACTGGGGGGA CACCCAAAGT CCCAGACTGT 
TTCCAAAGGA CACCTAAAAA GCTGTGCATT CCTGAAAAAA CTGAGATCCT GGCAGTCAAT 
GTAGATTCCA AAGGGGTTCA CGCGGTACTG AAAACAGGGA CATGGGTACG GTACTGTATC 
TTTGATCTTG CAACTGGAAA AGCAGAACAG GAAAACAACT TCCCAACAAG TAGCATTGCA 
TTTCTTGGCC AGAATGAGAG AAATGTTGCT ATTTTTACAG CTGGGCAGGA ATCTCCCATC 
ATTCTTAGAG ATGGCAATGG TACCATTTAT CCCATGGCAA AAGATTGCAT GGGTGGGATA 
AGAGATCCAG ACTGGCTTGA CCTTCCTCCA ATAAGCAGTC TTGGAATGGG AGTTCATTCT 
TTAACCAATC TCCCTGCAAA TTCAACAATT AAGAAGAAAG CAGCCATTAT CATAATGGCT 
GTTGAGAAGC AAACACTGAT GCAACACATT TTGCGCGGTG ATTATGAGGC CTGCAGGCAG 
TATTTGATGA ACCTAGAGCA AGGTGTAGTT TTGGATCCAA ACACTCAAAT GCTGCAGACT 
TTTCTGAGCC ACAGATGTGA TGGCAATAGG AACATATTGC ATGCCTGCGT GTCCGTTTGC 
TTCCCAACAA GCAACAAGGA AACAAAAGAA GAGGAGGAAG CAGAGCGCTC TGAAAGGAAT 
ACATTTGCAG AAAGACTGTC TGCAGTAGAA GCAATTGCCA ATGCTATATC TGTGGTTTCT 
AGCAATGGTC CCGGAAATCG TGCTGGATCG TCCAGCAGTA GAAGCTTGAG ATTGAGGGAA 
ATGATGAGGA GGTCCCTTCG GGCAGCAGGT TTAGGAAGAC ATGAGGCTGG TGCTTCTAGT 
GATCACCAAG ACCCAGTATC TCCTCCCATT GCACCTCCTA GCTGGGTTCC TGATCCACCT 
GCTATGGATC CAGATGGTGA CATAGATTTT ATCCTGGCCC CTGCTGTAGG ATCTCTTACC 
ACTGCATCCA CTGTACCTGG CCAAGGACCA AATACTTCTA CAATACCAGG TCCTTCCTCA 
GAGCCATCAG TTGTTGAGTC CAAGGATCGG AAGGCAAATG CTCACTTCAT ACTGAAGTTA 
TTATGCGATA GCATCGTTCT GCAGCCTTAC CTACGTGATC TCCTGTCTGC CAAGGATGCA 
AGAGGTATGA CTCCCTTCAT GTCTGCTGTT AGTGGAAGAG CCTATCCAGC TGCAATTACC 
ATACTGGAAA CTGCACAGAA AATCGCAAAA GCTGAAGCAA ATTCAAGTGA GAAAGAGGAG 
GATATTTTCA AGGGAATGGT ATGTCCACCT GGTACCAATG CAGACGACTC TCCTCTATAT 
GTACTTTGCT GCAATGATAC CTGCAGTTTT ACATGGACAG GAGCTGAGCA TATCAATCAG 
GACATATTTG AATGTCGAAC ATGTGGCTTG TTGGAATCCC TGTGCTGCTG TACCGAGTGT 
GCAAGAGTCT GCCACAAAGG CCATGACTGC AAATTGAAAA GAACATCACC TACTGCTTAC 
TGCGACTGTT GGGAGAAATG CAAATGCAAA ACATTAATTG CCGGGCAAAA GTCTGCACGA 
CTTGATCTGC TCTATCGACT GCTGACCATC ACTAATCTTG TTACAATGCC AAATAGCAGG 
GGAGAGCACC TACTTCTTTT CTTAGTGCAG ACTGTAGCCA GACAGACAGT GGAGCACTGC 
CAGTACCGAC CTCCAAGGAT TCGAGAAGAT CGCAATCGAA AGGCAGCTAA CCCTGAAGAT 
TCCGACATGC CAGATCATGA CCTAGAGCCC CCTAGGTTTG CCCAGCTTGC ACTGGAACGT 
GTTTTACAAG ATTGGAACGC ACTCAAATCC ATGATTATGT TTGGATCCCA AGAAAATAAA 
GACCCCCTTA GTGCTAGCAG TAGAATCGGG CATCTTCTTC CAGAGGAACA AGTTTACTTG 
AATCAGCAGA GTGGTGCCAT TCGCTTGGAC TGTTTTACTC ATTGCCTAAT TGTCAAGTGC 
ACAGCAGATA TTTTGCTGTT GGATACCTTG CTGGGAACCC TTGTAAAAGA ACTCCAAAAT 
AAATATACCC CTGGGCGTAG AGAAGAAGCT GTAGCTGTCA CAATGCGTTT TCTGCGTTCA 
GTGGCTCGTG TTTTTGTCAT TCTCAGTGTA GAAATGGCTT CATCCAAGAA GAAAAACAAT 
TTCATTCCAC AGCCAATAGG GAAATGCAAG CGAGTGTTCC AAGCTTTACT TCCATATGCA 
GTGGAGGAGT TGTGTAATGT TGCAGAATCC CTCATTGTCC CAGTGAGAAT GGGCATTGCA 
CGCCCCACTG CCCCCTTCAC ATTAGCAAGT ACAAGCATAG ATGCCATGCA GGGCAGCGAG 
GAGCTGTTCT CTGTGGAGCC TCTTCCTCCG AGACCTTCAT CTGATCAGTC TAACAGTTCG 
AATCAGTCCC AGTCATCCTA TATCATTAGG AATCCTCCGC AAAGAAGAAT AAGCCAGTCC 
CAGCCTGTGC GAGGCCGAGA TGAAGAGCAG GATGACATTG TATCAGCAGA TGTGGAAGAG 
GTTGAAGTGG TAGAGGGGGT AGCAGGAGAA GAGGACCATC ATGATGAGCA AGAAGAGCAT 
GGGGAGGAGA ATACAGAAGC AGAAGGACAA CATGATGAGC ACGATGAAGA TGGTAGTGAC 
ATGGAGTTAG ACCTTCTTGC TGCAGCTGAA ACAGAAAGTG ACAGTGAAAG TAACCACAGC 
AATCAGGATA ATGCCAGCGG TCGGAGGAGT GTAGTAACAG CAGCTACCGC TGGATCAGAA 
GCAGGTGCCA GCAGCGTTCC GGCCTTTTTC TCTGAAGATG ACTCCCAATC CAATGACTCC 
AGTGATTCTG ATAGCAGCAG CAGTCAAAGT GATGACCTGG ACCAGGAGAC CTTTATGATT 
GATGAGCCTC TAGAGAGGTC GTCCAACAGC AATCATGCAA ATGGGGCTGC CCAGGCTCCA 
AGGTCAATGC AGTGGGCAGT ACGGAACACT CAGAGCCAAA GAGCCCCAAA CACCACACCA 
TCTAATACTT CTACTCCAGC AGCCAGTTCT GGTGCCTTGA TTTACATTGA ACCATCCAGT 
CTGAGGCGCA CTGGCAACAT AAGCTCCAGT GCAGCAGCAG CAGCCGCAGC CCTTGAAGCA 
AGCAATTCTA GCAGTTATCT AACTTCAGCA AGCAGTCTGG CTAGAGCCTA CAGCATAGTG 
ATACGGCAGA TATCTGACTT GATGGGTCTA ATCCCCAAGT ACAACCACTT GGTCTATTCA 
CAGATCCCAG CAGCCGTCAA ACTGAACTAT CAAGATGCCG TTAACTTACA GAACTATGTT 
GAAGAAAAAC TTATTCCCAC ATGGAACTGG ATGGTGAGCA TAATGGACTC CACTGAAGCC 
CAGCTCCGTT ATGGCTCCGC CTTATCATCC GCTGGAGATC CAGGTCATCC CAACCATCCT 
CTTCATGCAT CTCAGAATTC CAACCGCAGG GAAAGGATGA CTGCCCGTGA GGAAGCTAGT 
TTGAGGACTC TTGAGGGAAG AAGAAGAGCC ACTCTGCTTA CTGCTCGACA GGGGATGTCG 
GCCCGTGGAG ACTTCCTGAA TTATGCACTT TCACTAATGC GTTCGCACAA TGATGAGCAC 
TCTGATGTAC TCCCTGTTTT GGATGTTTGC TCCCTGAAAC ACGTGGCCTA TGTCTTCCAG 
GCTCTGATAT ACTGGATTAA GGCAATGAAC CAACAAACAA CATTGGATAC TCCACAGCTG 
GAAAGAAAGA GGACAAGAGA GCTTTTGGAG CTTGGCCTTG ATAATGAAGA TTCAGAGCAT 
GAAAATGATG ATGACACAAA TCAAAGCCTA ATATTGAATG ACAAAGACGA CGATACACTA 
CCCTCTGAGA CCGGTCCAAA CCATCCGTTT TTCCGTCGTT CCGATTCAAT GACGTTCCTT 
GGTTGTATTC CACCAAACCC CTTTGAAGTT CCTCTGGCAG AAGCTATCCC ACTTGCAGAC 
CAGCCCCATC TTTTGCAGCC AAATGCAAGG AAAGAAGACT TATTTGGTCG ACCGAGTCAA 
GGTCTCTACT CATCTACTGC TAACACTGGA AAATGCCTAT TAGAAGTGAC AGTTGACAGA 
AACTGCCTGG AGGTTTTACC AACCAAAATG TCATATGCTG CTAATCTAAA AAATGTAATG 
AACATGCAGA GCCGTCAGAA AGAAGCAGAG GAACAGAATG TGCCGGCTGA GGAAAATGAC 
ACATCAAAGG CTGGACCCTC AGCTCATGAT CTTGCAGCTC AGCTGAAAAG TAGCTTGTTG 
GCAGAAATAG GGTTAACAGA GAGTGAAGGT CCCCCGCTTA CATCATTCAG GCCACAGTGT 
AGCTTTATGG GAATGGTCAT TTCCCATGAT ATGCTGTTAG GACGTTGGCG ACTATCTCTT 
GAATTGTTTG GAAGGGTGTT TATGGAAGAT GTCGGAGCAG AGCCTGGATC GATCTTAACT 
GAACTAGGAG GGTTTGAAGT AAAGGAATCC AAGTTCCGCA GGGAGATGGA AAAACTTCGA 
AATCAGCAGT CCAGAGATCT TACACTAGAG GTGGATCGAG ATAGAGATCT TCTAATACAG 
CAAACGATGA GGCAGCTGAA TAATCATTTT GGGCGCAGAT GCGCCACAAC TCCTATGGCA 
GTGCATAGAG TAAAGGTCAC CTTTAAAGAT GAGCCAGGAG AAGGCAGTGG AGTAGCCCGA 
AGTTTTTATA CTGCAGTAGC ACAAGCATTT TTGTCCAATG AAAAGTTGCC AAGCTTGGAC 
TGTATCCAAA ATGCCAACAA AGGTACTCAT ACAAGTTTGA TGCAAAGACT CAGAAATCGT 
GGGGAACGAG ACCGGGAAAG GGAGCGGGAC ATGAGAAGAA GCAGTGGTTT GCGAACAGGT 
TCTCGGCGGG ATAGAGACAG AGATTTCAGA CGGCAACTTT CCATTGATAC TAGGCCTTTC 
AGGCCAGCTT CTGAGGGCAA TCCTAGTGAT GATCCTGATC CTCTTCCTGC TCACCGACAG 
GCTCTCGGAG AAAGACTATA CCCACGTGTA CAAGCCATGC AGCCGGCATT TGCAAGTAAA 
ATTACAGGCA TGCTGCTTGA GCTATCTCCT GCACAACTGC TGCTGCTGTT GGCAAGTGAA 
GACTCTCTAA GAGCCAGAGT GGATGAAGCT ATGGAACTTA TTATTGCACA TGGAAGGGAG 
AATGGTGCTG ACAGTATCCT CGATTTAGGT TTATTAGAAG CAGCTGATAA GGTACAGGAA 
AACCGTAAGC GCCATGGCTC TAGTCGAAGC GTGGTTGATA TGGAGCTTAC TGACACAGAC 
GATGGGGACG ATAATGCTCC TTTATTTTAC CAACCTGGTA AACGAGGTTT CTATAGTCCA 
AGGCCTGGAA AAAACACAGA GGCACGTTTA AACTGTTTCC GCAACATCGG CAGAATTCTT 
GGTCTGTGCC TACTACAGAA TGAATTATGT CCTATCACTT TAAGTAGGCA GGTGATTAAA 
GTGCTTCTGG GCAGAAAGGT GAACTGGCAT GATTTTGCGT TCTTTGATCA CGTGATGTAC 
GAGAGCTTAC GGCAACTTAT ACTCGCCTCC CAAAGCCCGG ATGCTGACGC AGTGTTTGCC 
TCAATGGACC TAGCGTTTGC AATAGACCTC TGCAAAGAAG AAGGTGGTGG ACAAGTGGAA 
CTTATTCCTA ATGGCGTAAA TATCCCTGTA ACCCCTCAAA ATGTGTATGA ATATGTAAGA 
AAATATGCTG AACACAGAAT GCTCACTGTA GCAGAGCAAC CGCTTAATGC CATGAGGAAA 
GGACTATTGG ATGTTCTCCC AAAGAATGCA TTAGAAGACT TAACTGCAGA AGACTTTAGG 
CTCTTAGTGA ATGGCTGTGG GGAAGTTAAT GTTCAGATGC TCATTAGCTT TACCTCCTTC 
AACGATGAGT CAGGAGAAAA CGCAGAAAAG CTGTTGCAGT TCAAACGCTG GTTCTGGTCA 
ATCGTAGAGA AGATGAGTAT GACAGAGCGG CAAGATTTAG TTTATTTTTG GACCTCAAGC 
CCATCATTGC CTGCGAGTGA AGAGGGATTC CAGCCCATGC CATCCATTAC CATCCGGCCC 
CCCGATGATC AGCACCTTCC CACGGCGAAC ACCTGCATCT CTCGCCTCTA CGTCCCCCTT 
TATTCTTCCA AACAGATCCT GAAACAGAAG TTGTTACTTG CTATTAAGAC CAAGAACTTT 
GGATTTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC108695408 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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