LOC660029 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC660029
Entrez Gene ID	660029
Full Name	methyl-CpG-binding domain protein 5
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015985198.1	XP_015840684.1	methyl-CpG-binding domain protein 5 isoform X1
XM_008202830.2	XP_008201052.1	methyl-CpG-binding domain protein 5 isoform X1
XM_015985199.1	XP_015840685.1	methyl-CpG-binding domain protein 5 isoform X3
XM_008202831.2	XP_008201053.1	methyl-CpG-binding domain protein 5 isoform X4
XM_966293.3	XP_971386.1	methyl-CpG-binding domain protein 5 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC660029 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC660029 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTg01696	XM_008202830.2 Latest version!	Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTg01696	XM_015985198.1 Latest version!	Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTg01697	XM_966293.3 Latest version!	Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTg01698	XM_008202831.2 Latest version!	Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTg17004	XM_015985199.1 Latest version!	Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC660029 ( XM_015985198.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015840684.1 Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTg01696
Clone ID Related Accession (Same CDS sequence)	XM_015985198.1 , XM_008202830.2
Accession Version	XM_015985198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	methyl-CpG-binding domain protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450135.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA AGCCTGAGCT GTCGCCCGCC CCCGGCGTCC AGAATGTGGT AGTGTACGTT 
TCGAACGACA ACTTCGCCCA AGCGTACGCC GTCGGGGGGC AGAATGCGCC CCAGTACGGC 
CCCCCGAAGC CCTACCCCGA GAAGCGGCCC GACTACCCCG AGGGCTACTT CGTCTCGTCC 
AACGGCTACG GATACGAGGA GCGCCCGGCC AAGGAGTGCG ACTACGACCC GGCCTATGTG 
ACGGTCATGG GGGCCGAGGG GCCCCCGCAG AGTGTGCGGT GTGATTCAGT GCGGAGCGAG 
ACGGCCGAGT CGAGCTGCAG CAGCTTGAGC TCGGCCGACG AGGGGATGGT GCTAGTACAG 
AACAATCAAC CAGAGATGGT GGTGTATGAT CCCAGTGTTA GTGTTAGACC CGGTGTCGTG 
TTTGTGGGGG CCGGCGTCCA CCACCACCCC AATGTCGCCG CCACCGCCGC CGCCAACCTA 
CCCGCCGCCC AACAGCCCTC CATCACTGTG CCCTACGGCT GGAAGAGGAT TCTCAACAAC 
GGATCCATCA TGTACATAAG CCCAAGTAAC ACAGCTTTAA GTTCTCCGGA ACAAGTCAAA 
GAATATCTCC TCACGTCGGG CACTTGCAAG TGTGGATTAG AATGTCATTT CAAATATGAC 
AACACTTTTA ATTTCGATCC TAAGGTGGCC AGTAAACCGT GGACGCTATC ACCGGACACC 
AATACAGGGG ACCTGACCAA ATTGTGCAAC CACAAGAGAA AAATCATCGC CATGGCCTCG 
TTGGAGTGCA AGCCCCCGGA CCCCGCCGCC AAAATGCGAA AAGACTGCAA CAATAAGAAA 
ACCAAGCGAA AAGTCGGCAT GCCTTACGCC GGTTGTGTTT CTTTATCGCA AATTTTCACT 
CGTCCGGACA AACTCGCTTT GAGTCAACAG CATTTCAACA AGGAAGGTCT CGGAAATCAA 
GCTCAGGTCG TGTGGCCTCA GTCACCGACT AGCAGCTCTC AAATGGGTAA TCGTCTGCAG 
AATTACCCCG AGAGCCAGCA GATGGTCCGA TATCAAAACC AGGAGCAACA GAATCGAATC 
GTTAACGGTT CGCCCATGGT GGTCGGTGAT AACAGTACCA TGATGGTACC CCAACAACAG 
TCCAACATAT CGCAACAGAT CCACCAGAAC CACAATGGTC AAAATCCCAG CGTTCCTCAG 
CAGCACATTA TCGGGCCCAA CGGCCAAGTG ATAAGTCTCC AAGGGGCCCT TAACTCGAAT 
CAGGTCAATA ATGGAGTGGT CATACAGGGT CAAAACGTCG TTCCTAATCC AAATCAATCC 
CGAGTTTTCA TCAACAGCCA ACCAAGCGAA CCCAGTGGAC CAGGCCGGCC CAACATCCAG 
ATGATTCCCG TAGGAGTCAC GAATCAAAGA GATGCCCAAA ACCGCCTCCA CCACTTCTAC 
AACAACCAAC AATACCCACA GATACAATAC GACGCGTCGA AGCAAATGCG ACCGACATTT 
ATGCCCGGCC ACAACCAAAT GAACGGTTTT CGCCCGCAAA ATAATCAGAT TATGCAAGCG 
CCGGTTATGA TTAACAACCA TCAAAAGATG CCATGGCCTA ATCGCATTCA AAATTCGCCC 
AATGCCTACG AAAGAGTGCC GCCGCTGCAT CCACACACAC CATCGCCCAC TATGTGGCAA 
GACGAGGTCA AACGGAAAAA GGTCAAATTG GGGAAAATCG TTAAAAATCG GCCGTATCAT 
ATGATGGATT CGGCGTTGCA TGCACCCTGT CCCAACATTG ACGTGCGGCA AATACCAGCC 
GAAAACGGCC GACAGGTTAT TATCAACCAG AATTCGGCCA GTCCCTCGTT TATGGAGGAC 
CCCAGTGGGT ATTTAGCCCA GCAGACGGCC CTGCTCAATA ATACCATCAA CAGACAAACA 
GGGGTGAACG CGTGCGCGGG CATGGTGTGC AGCAGCCCGC AGCAGCCCCA CCTAACACCA 
CCGAATTCGC AAACAAACGA GGTGCCTTTA CCCAGCAACG TGGTGATATC TCATATGAAA 
CAAACGCAAA ACATTACAAA TCGAACCAAT CAAACGTTAC ACGTAGTAAA AAGTCAAGTT 
CCTCAAGTAA TGTCCCAGCA GTATAATCAA ATGATGGCGG CTCACGCCGA CAGTAGCGTC 
ACCAACGACC AATGTCAAAA TTGCGACAGT CATAAAGGCC ATTCGAGACC TAACAGTCAA 
CCTGGAACTC CGAGTTCGTC GGCTATTGAC GATCCGGTGA CATCATCGAC TTATTCCGAC 
AAACAAAATA ATCAATCACC GGATTCAAGA CCGATTCAGG GCGGTACTGT TAGTACTAGC 
AATGTATCGC CATTGGATGG ATCACAATCA GATCCACCTA CACCTAACCC ACACACTCCA 
ACGCCTCCTA GGAATGATAA TATGCCATAT GCTGTCTACC AATCGCGTGA GGTTTGCAGT 
ACGAATTACC CACCGAAAAG TGACTGTTGT TCGAAAATGA ACACGCCGTA TATTTCGAGT 
ATAGTTACGA CGATGGCAAG TGGACGAACA GTTGGTAGTA ATACCATAAC GTCGGTTTTG 
GCCGGTCGTG CCAACACTGC CACAGTTTCA GTCAACAGTC CTGCTAACCT CCCAGCATCC 
ACAACCCCAA CAACAGTCAC ACAAACCGTT TCAAAATCCC CACTTGAAAT GGTCCAAAGC 
GTCGTCAGTA GCATACAAGT CCCTCAGTCT CAAACCAATC CCCAAATCTC TCCACCACAT 
ATCGTCAAAC ATTCCCCCAC CGGGTTACCT CCTGGCCATA TCCTTGTCTC AAGCGGCGGC 
CAGTTAATAA TGGCAAACAC CGGAAACAGC CAATCGGGCG TTATGCCACC CCCACCCCCC 
AAACTAGTCT CCAATCAAAC CGCAATGCCG CCAATGTCCG TCTCACCAAT GATCACAAAT 
GTCACTGCAG CGGTCAGTCA GGTCATACCG GCAGTAGCTC AGCAAGTTTT AGGCCAACAA 
ACCGTTTTAG TGAACGCCTT ACCCACACCT TTTGTCTTAC AAGGCGGAGT CACAATGACC 
ATGGACGGGT CCATGGTCGG CCAAAACATG CAATTACCTC AAATCGTCGC CGGAAATGTG 
ATACAACAAC AAATCCAACT GGATAACAGT GACCCACGAC GTACCACCGC GCTACTCTCA 
CCCGAAACCA AGAAAAAAGG AAAGAAACGA AAAATGTCAC AAACAGTGGC CAACATGCTT 
CACATAGCTG CCCAGCAAAA CTCCGGCGTT GTGATGTCAC AACAAGGCTT CCCGCAGCAA 
ATTCAAATGG CTCATAGTCC GCAAGGTCTA GCCACGGGCC CAGTGATGCA AGCCTTGACC 
ATAGTCCCCA GCAAAACAGG GGGACCCCCA CAGATCGTAA TGAACGGGCA ACCCATGACC 
AACACCAGTC CCCAACTCAT TGCAAACTCG CAACCGGCGC AACAAATCAA CCTCCTTCAG 
CCGGTGAATC TCATCAATGG CACCACCGGA GTGGTGCAAA ATTTCCCCAC GATTCAACAA 
TTCATAGTCC CGAATTTAGG CGGCATGGTT ATGAACCCGG ACGGCACCGC CACTATATTA 
CAGGACACTT CAAATATCGG CATGCAGTTA CAACTACAGA ACGTTAACGG ACAAAACGTC 
CTGACTCCGG TCCAAAACAA CGGAATGTTC AACGGGGGTC AGAGTATTCT AGCAGCGAGT 
CCGGCCGGCA TGGTCATTAG GGCCCCCACC TCGCAGGGGA AATTAATCCA GCAGCAGCAG 
CACAGTCCAG GTGCCCAGTT TTTGTCACCC AACGGGGGCC AGTTTGTCGT CAATGGGACA 
CAATTCAGCG GCCAGTTGAG CCCGCTTGTG GCTAGTGTGA GTCCGAGTCA GCAAGTTACC 
TTTGGGACGT CCACGCCGCA GATTAGACCA ACTCAGGGTC AACAAGAATT TATACAGTGT 
GGTCAAATGG GCCAGACTTT GATGGTTCCG TGTGCACCAA CGGTCGCCGT TTCCTCCTCG 
CAAAACACGA CTTTTGTCCA GCAAAACACT ACGATTGTTC AGCAGCAAAC CACGATGGTT 
TCGAATAGTC AACAGTTACA GAATTTCCAG AATAATGGAG GGAATCGAAC GACTGTGAAT 
GTGGATCAGA ATTTTATCAT CGCGAATGAT AATAAGCAAG TGCAGGCGCT TGTTCAGGTT 
CAGAGGGAGA GTCCCGGGAC GAATTATAGA CAGTCGGTGT CGACGCAAAC AGCGGTTAAT 
CAGAATGCAC AGACAGTGAC CACGTTTTGT CAGACGTCCA CGCTGTGTGC GGGGAGTCCC 
CCGGATACTA CAACGCATAG TCCGTTGGCG TCGGGAGGAC AGAGTCCTGC AACGGCCGAT 
ACGACCACAC ATACGGGAAG TACAGACGAT GGGCTGTCGC CAGCGCCGTC GAATTGTTCG 
GTGACGTGCG ATAGTGCAGG GAGACAGCCG GCTTGCACGA TGGCGATGGT GCACTGCATA 
TCCAGCAGCG AGCCCGATTC GGCTGATCTG AACACCCAAG GCTCTGACCA TGACTGGTCC 
CGAGCGTCGT CTATCCCTCA GCAAAAAAGC GACTTGTCGG AGATACATTT CCCCAAAAAA 
CAAAACACTC CGACCCACGT GGCTTACGCC GAATCGTCGA CAATGATGGC CGGTTTGCAT 
TTTATCGGCG ATCAAATGAA GGCCCAAGAG GCCGTCGTCA GTTCTCACAA TTTCGATAAA 
ATCGTACAGA AACGCAAAAG CAGCGATACT TTGCTCGTCA TGGAAACCCA TCACATGCAT 
TCGTCGCTTT TCGACGAGGA GGAAGATAAT GAGAAACGTG AAAAGTCAGA ACATGACTTT 
GTTGTTGGCG ATTTGGTTTG GGGGCCGGCT AAAGCAAGTC CAGCTTGGCC TGGCAAAATA 
ATACAAGTCG ACGGGAACGA TGTTTCGGTT AAATGGTTCG GCAGCGAAAA ATTAATTACA 
AAATTAGACA GAGGTTCCTT GCAGACACTC ACTGAAGGTC TCGATGCTCA CCACCAAGCG 
CGCAAAAAAT GCAGAACATC TCGCAAACTG AATTCTCATC TGGAGAAGGC CATCCAAGAG 
GCCATGGCTG AGCTGGATAA GGACTTGGAG CAAGAGACGA AGGAGGTGAA GCAGCAGAAG 
AAGAAAGCGC ACCGAGTGAG AAGCGGTCAT CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015840684.1
CDS	311..5506
Translation	MAGKPELSPAPGVQNVVVYVSNDNFAQAYAVGGQNAPQYGPPKPYPEKRPDYPEGYFVSSNGYGYEERPAKECDYDPAYVTVMGAEGPPQSVRCDSVRSETAESSCSSLSSADEGMVLVQNNQPEMVVYDPSVSVRPGVVFVGAGVHHHPNVAATAAANLPAAQQPSITVPYGWKRILNNGSIMYISPSNTALSSPEQVKEYLLTSGTCKCGLECHFKYDNTFNFDPKVASKPWTLSPDTNTGDLTKLCNHKRKIIAMASLECKPPDPAAKMRKDCNNKKTKRKVGMPYAGCVSLSQIFTRPDKLALSQQHFNKEGLGNQAQVVWPQSPTSSSQMGNRLQNYPESQQMVRYQNQEQQNRIVNGSPMVVGDNSTMMVPQQQSNISQQIHQNHNGQNPSVPQQHIIGPNGQVISLQGALNSNQVNNGVVIQGQNVVPNPNQSRVFINSQPSEPSGPGRPNIQMIPVGVTNQRDAQNRLHHFYNNQQYPQIQYDASKQMRPTFMPGHNQMNGFRPQNNQIMQAPVMINNHQKMPWPNRIQNSPNAYERVPPLHPHTPSPTMWQDEVKRKKVKLGKIVKNRPYHMMDSALHAPCPNIDVRQIPAENGRQVIINQNSASPSFMEDPSGYLAQQTALLNNTINRQTGVNACAGMVCSSPQQPHLTPPNSQTNEVPLPSNVVISHMKQTQNITNRTNQTLHVVKSQVPQVMSQQYNQMMAAHADSSVTNDQCQNCDSHKGHSRPNSQPGTPSSSAIDDPVTSSTYSDKQNNQSPDSRPIQGGTVSTSNVSPLDGSQSDPPTPNPHTPTPPRNDNMPYAVYQSREVCSTNYPPKSDCCSKMNTPYISSIVTTMASGRTVGSNTITSVLAGRANTATVSVNSPANLPASTTPTTVTQTVSKSPLEMVQSVVSSIQVPQSQTNPQISPPHIVKHSPTGLPPGHILVSSGGQLIMANTGNSQSGVMPPPPPKLVSNQTAMPPMSVSPMITNVTAAVSQVIPAVAQQVLGQQTVLVNALPTPFVLQGGVTMTMDGSMVGQNMQLPQIVAGNVIQQQIQLDNSDPRRTTALLSPETKKKGKKRKMSQTVANMLHIAAQQNSGVVMSQQGFPQQIQMAHSPQGLATGPVMQALTIVPSKTGGPPQIVMNGQPMTNTSPQLIANSQPAQQINLLQPVNLINGTTGVVQNFPTIQQFIVPNLGGMVMNPDGTATILQDTSNIGMQLQLQNVNGQNVLTPVQNNGMFNGGQSILAASPAGMVIRAPTSQGKLIQQQQHSPGAQFLSPNGGQFVVNGTQFSGQLSPLVASVSPSQQVTFGTSTPQIRPTQGQQEFIQCGQMGQTLMVPCAPTVAVSSSQNTTFVQQNTTIVQQQTTMVSNSQQLQNFQNNGGNRTTVNVDQNFIIANDNKQVQALVQVQRESPGTNYRQSVSTQTAVNQNAQTVTTFCQTSTLCAGSPPDTTTHSPLASGGQSPATADTTTHTGSTDDGLSPAPSNCSVTCDSAGRQPACTMAMVHCISSSEPDSADLNTQGSDHDWSRASSIPQQKSDLSEIHFPKKQNTPTHVAYAESSTMMAGLHFIGDQMKAQEAVVSSHNFDKIVQKRKSSDTLLVMETHHMHSSLFDEEEDNEKREKSEHDFVVGDLVWGPAKASPAWPGKIIQVDGNDVSVKWFGSEKLITKLDRGSLQTLTEGLDAHHQARKKCRTSRKLNSHLEKAIQEAMAELDKDLEQETKEVKQQKKKAHRVRSGHR

Target ORF information:

RefSeq Version	XM_015985198.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015985198.1

ORF Insert Sequence:

ATGGCAGGAA AGCCTGAGCT GTCGCCCGCC CCCGGCGTCC AGAATGTGGT AGTGTACGTT 
TCGAACGACA ACTTCGCCCA AGCGTACGCC GTCGGGGGGC AGAATGCGCC CCAGTACGGC 
CCCCCGAAGC CCTACCCCGA GAAGCGGCCC GACTACCCCG AGGGCTACTT CGTCTCGTCC 
AACGGCTACG GATACGAGGA GCGCCCGGCC AAGGAGTGCG ACTACGACCC GGCCTATGTG 
ACGGTCATGG GGGCCGAGGG GCCCCCGCAG AGTGTGCGGT GTGATTCAGT GCGGAGCGAG 
ACGGCCGAGT CGAGCTGCAG CAGCTTGAGC TCGGCCGACG AGGGGATGGT GCTAGTACAG 
AACAATCAAC CAGAGATGGT GGTGTATGAT CCCAGTGTTA GTGTTAGACC CGGTGTCGTG 
TTTGTGGGGG CCGGCGTCCA CCACCACCCC AATGTCGCCG CCACCGCCGC CGCCAACCTA 
CCCGCCGCCC AACAGCCCTC CATCACTGTG CCCTACGGCT GGAAGAGGAT TCTCAACAAC 
GGATCCATCA TGTACATAAG CCCAAGTAAC ACAGCTTTAA GTTCTCCGGA ACAAGTCAAA 
GAATATCTCC TCACGTCGGG CACTTGCAAG TGTGGATTAG AATGTCATTT CAAATATGAC 
AACACTTTTA ATTTCGATCC TAAGGTGGCC AGTAAACCGT GGACGCTATC ACCGGACACC 
AATACAGGGG ACCTGACCAA ATTGTGCAAC CACAAGAGAA AAATCATCGC CATGGCCTCG 
TTGGAGTGCA AGCCCCCGGA CCCCGCCGCC AAAATGCGAA AAGACTGCAA CAATAAGAAA 
ACCAAGCGAA AAGTCGGCAT GCCTTACGCC GGTTGTGTTT CTTTATCGCA AATTTTCACT 
CGTCCGGACA AACTCGCTTT GAGTCAACAG CATTTCAACA AGGAAGGTCT CGGAAATCAA 
GCTCAGGTCG TGTGGCCTCA GTCACCGACT AGCAGCTCTC AAATGGGTAA TCGTCTGCAG 
AATTACCCCG AGAGCCAGCA GATGGTCCGA TATCAAAACC AGGAGCAACA GAATCGAATC 
GTTAACGGTT CGCCCATGGT GGTCGGTGAT AACAGTACCA TGATGGTACC CCAACAACAG 
TCCAACATAT CGCAACAGAT CCACCAGAAC CACAATGGTC AAAATCCCAG CGTTCCTCAG 
CAGCACATTA TCGGGCCCAA CGGCCAAGTG ATAAGTCTCC AAGGGGCCCT TAACTCGAAT 
CAGGTCAATA ATGGAGTGGT CATACAGGGT CAAAACGTCG TTCCTAATCC AAATCAATCC 
CGAGTTTTCA TCAACAGCCA ACCAAGCGAA CCCAGTGGAC CAGGCCGGCC CAACATCCAG 
ATGATTCCCG TAGGAGTCAC GAATCAAAGA GATGCCCAAA ACCGCCTCCA CCACTTCTAC 
AACAACCAAC AATACCCACA GATACAATAC GACGCGTCGA AGCAAATGCG ACCGACATTT 
ATGCCCGGCC ACAACCAAAT GAACGGTTTT CGCCCGCAAA ATAATCAGAT TATGCAAGCG 
CCGGTTATGA TTAACAACCA TCAAAAGATG CCATGGCCTA ATCGCATTCA AAATTCGCCC 
AATGCCTACG AAAGAGTGCC GCCGCTGCAT CCACACACAC CATCGCCCAC TATGTGGCAA 
GACGAGGTCA AACGGAAAAA GGTCAAATTG GGGAAAATCG TTAAAAATCG GCCGTATCAT 
ATGATGGATT CGGCGTTGCA TGCACCCTGT CCCAACATTG ACGTGCGGCA AATACCAGCC 
GAAAACGGCC GACAGGTTAT TATCAACCAG AATTCGGCCA GTCCCTCGTT TATGGAGGAC 
CCCAGTGGGT ATTTAGCCCA GCAGACGGCC CTGCTCAATA ATACCATCAA CAGACAAACA 
GGGGTGAACG CGTGCGCGGG CATGGTGTGC AGCAGCCCGC AGCAGCCCCA CCTAACACCA 
CCGAATTCGC AAACAAACGA GGTGCCTTTA CCCAGCAACG TGGTGATATC TCATATGAAA 
CAAACGCAAA ACATTACAAA TCGAACCAAT CAAACGTTAC ACGTAGTAAA AAGTCAAGTT 
CCTCAAGTAA TGTCCCAGCA GTATAATCAA ATGATGGCGG CTCACGCCGA CAGTAGCGTC 
ACCAACGACC AATGTCAAAA TTGCGACAGT CATAAAGGCC ATTCGAGACC TAACAGTCAA 
CCTGGAACTC CGAGTTCGTC GGCTATTGAC GATCCGGTGA CATCATCGAC TTATTCCGAC 
AAACAAAATA ATCAATCACC GGATTCAAGA CCGATTCAGG GCGGTACTGT TAGTACTAGC 
AATGTATCGC CATTGGATGG ATCACAATCA GATCCACCTA CACCTAACCC ACACACTCCA 
ACGCCTCCTA GGAATGATAA TATGCCATAT GCTGTCTACC AATCGCGTGA GGTTTGCAGT 
ACGAATTACC CACCGAAAAG TGACTGTTGT TCGAAAATGA ACACGCCGTA TATTTCGAGT 
ATAGTTACGA CGATGGCAAG TGGACGAACA GTTGGTAGTA ATACCATAAC GTCGGTTTTG 
GCCGGTCGTG CCAACACTGC CACAGTTTCA GTCAACAGTC CTGCTAACCT CCCAGCATCC 
ACAACCCCAA CAACAGTCAC ACAAACCGTT TCAAAATCCC CACTTGAAAT GGTCCAAAGC 
GTCGTCAGTA GCATACAAGT CCCTCAGTCT CAAACCAATC CCCAAATCTC TCCACCACAT 
ATCGTCAAAC ATTCCCCCAC CGGGTTACCT CCTGGCCATA TCCTTGTCTC AAGCGGCGGC 
CAGTTAATAA TGGCAAACAC CGGAAACAGC CAATCGGGCG TTATGCCACC CCCACCCCCC 
AAACTAGTCT CCAATCAAAC CGCAATGCCG CCAATGTCCG TCTCACCAAT GATCACAAAT 
GTCACTGCAG CGGTCAGTCA GGTCATACCG GCAGTAGCTC AGCAAGTTTT AGGCCAACAA 
ACCGTTTTAG TGAACGCCTT ACCCACACCT TTTGTCTTAC AAGGCGGAGT CACAATGACC 
ATGGACGGGT CCATGGTCGG CCAAAACATG CAATTACCTC AAATCGTCGC CGGAAATGTG 
ATACAACAAC AAATCCAACT GGATAACAGT GACCCACGAC GTACCACCGC GCTACTCTCA 
CCCGAAACCA AGAAAAAAGG AAAGAAACGA AAAATGTCAC AAACAGTGGC CAACATGCTT 
CACATAGCTG CCCAGCAAAA CTCCGGCGTT GTGATGTCAC AACAAGGCTT CCCGCAGCAA 
ATTCAAATGG CTCATAGTCC GCAAGGTCTA GCCACGGGCC CAGTGATGCA AGCCTTGACC 
ATAGTCCCCA GCAAAACAGG GGGACCCCCA CAGATCGTAA TGAACGGGCA ACCCATGACC 
AACACCAGTC CCCAACTCAT TGCAAACTCG CAACCGGCGC AACAAATCAA CCTCCTTCAG 
CCGGTGAATC TCATCAATGG CACCACCGGA GTGGTGCAAA ATTTCCCCAC GATTCAACAA 
TTCATAGTCC CGAATTTAGG CGGCATGGTT ATGAACCCGG ACGGCACCGC CACTATATTA 
CAGGACACTT CAAATATCGG CATGCAGTTA CAACTACAGA ACGTTAACGG ACAAAACGTC 
CTGACTCCGG TCCAAAACAA CGGAATGTTC AACGGGGGTC AGAGTATTCT AGCAGCGAGT 
CCGGCCGGCA TGGTCATTAG GGCCCCCACC TCGCAGGGGA AATTAATCCA GCAGCAGCAG 
CACAGTCCAG GTGCCCAGTT TTTGTCACCC AACGGGGGCC AGTTTGTCGT CAATGGGACA 
CAATTCAGCG GCCAGTTGAG CCCGCTTGTG GCTAGTGTGA GTCCGAGTCA GCAAGTTACC 
TTTGGGACGT CCACGCCGCA GATTAGACCA ACTCAGGGTC AACAAGAATT TATACAGTGT 
GGTCAAATGG GCCAGACTTT GATGGTTCCG TGTGCACCAA CGGTCGCCGT TTCCTCCTCG 
CAAAACACGA CTTTTGTCCA GCAAAACACT ACGATTGTTC AGCAGCAAAC CACGATGGTT 
TCGAATAGTC AACAGTTACA GAATTTCCAG AATAATGGAG GGAATCGAAC GACTGTGAAT 
GTGGATCAGA ATTTTATCAT CGCGAATGAT AATAAGCAAG TGCAGGCGCT TGTTCAGGTT 
CAGAGGGAGA GTCCCGGGAC GAATTATAGA CAGTCGGTGT CGACGCAAAC AGCGGTTAAT 
CAGAATGCAC AGACAGTGAC CACGTTTTGT CAGACGTCCA CGCTGTGTGC GGGGAGTCCC 
CCGGATACTA CAACGCATAG TCCGTTGGCG TCGGGAGGAC AGAGTCCTGC AACGGCCGAT 
ACGACCACAC ATACGGGAAG TACAGACGAT GGGCTGTCGC CAGCGCCGTC GAATTGTTCG 
GTGACGTGCG ATAGTGCAGG GAGACAGCCG GCTTGCACGA TGGCGATGGT GCACTGCATA 
TCCAGCAGCG AGCCCGATTC GGCTGATCTG AACACCCAAG GCTCTGACCA TGACTGGTCC 
CGAGCGTCGT CTATCCCTCA GCAAAAAAGC GACTTGTCGG AGATACATTT CCCCAAAAAA 
CAAAACACTC CGACCCACGT GGCTTACGCC GAATCGTCGA CAATGATGGC CGGTTTGCAT 
TTTATCGGCG ATCAAATGAA GGCCCAAGAG GCCGTCGTCA GTTCTCACAA TTTCGATAAA 
ATCGTACAGA AACGCAAAAG CAGCGATACT TTGCTCGTCA TGGAAACCCA TCACATGCAT 
TCGTCGCTTT TCGACGAGGA GGAAGATAAT GAGAAACGTG AAAAGTCAGA ACATGACTTT 
GTTGTTGGCG ATTTGGTTTG GGGGCCGGCT AAAGCAAGTC CAGCTTGGCC TGGCAAAATA 
ATACAAGTCG ACGGGAACGA TGTTTCGGTT AAATGGTTCG GCAGCGAAAA ATTAATTACA 
AAATTAGACA GAGGTTCCTT GCAGACACTC ACTGAAGGTC TCGATGCTCA CCACCAAGCG 
CGCAAAAAAT GCAGAACATC TCGCAAACTG AATTCTCATC TGGAGAAGGC CATCCAAGAG 
GCCATGGCTG AGCTGGATAA GGACTTGGAG CAAGAGACGA AGGAGGTGAA GCAGCAGAAG 
AAGAAAGCGC ACCGAGTGAG AAGCGGTCAT CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC660029 ( XM_008202830.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008201052.1 Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTg01696
Clone ID Related Accession (Same CDS sequence)	XM_015985198.1 , XM_008202830.2
Accession Version	XM_008202830.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	methyl-CpG-binding domain protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450135.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008202830.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA AGCCTGAGCT GTCGCCCGCC CCCGGCGTCC AGAATGTGGT AGTGTACGTT 
TCGAACGACA ACTTCGCCCA AGCGTACGCC GTCGGGGGGC AGAATGCGCC CCAGTACGGC 
CCCCCGAAGC CCTACCCCGA GAAGCGGCCC GACTACCCCG AGGGCTACTT CGTCTCGTCC 
AACGGCTACG GATACGAGGA GCGCCCGGCC AAGGAGTGCG ACTACGACCC GGCCTATGTG 
ACGGTCATGG GGGCCGAGGG GCCCCCGCAG AGTGTGCGGT GTGATTCAGT GCGGAGCGAG 
ACGGCCGAGT CGAGCTGCAG CAGCTTGAGC TCGGCCGACG AGGGGATGGT GCTAGTACAG 
AACAATCAAC CAGAGATGGT GGTGTATGAT CCCAGTGTTA GTGTTAGACC CGGTGTCGTG 
TTTGTGGGGG CCGGCGTCCA CCACCACCCC AATGTCGCCG CCACCGCCGC CGCCAACCTA 
CCCGCCGCCC AACAGCCCTC CATCACTGTG CCCTACGGCT GGAAGAGGAT TCTCAACAAC 
GGATCCATCA TGTACATAAG CCCAAGTAAC ACAGCTTTAA GTTCTCCGGA ACAAGTCAAA 
GAATATCTCC TCACGTCGGG CACTTGCAAG TGTGGATTAG AATGTCATTT CAAATATGAC 
AACACTTTTA ATTTCGATCC TAAGGTGGCC AGTAAACCGT GGACGCTATC ACCGGACACC 
AATACAGGGG ACCTGACCAA ATTGTGCAAC CACAAGAGAA AAATCATCGC CATGGCCTCG 
TTGGAGTGCA AGCCCCCGGA CCCCGCCGCC AAAATGCGAA AAGACTGCAA CAATAAGAAA 
ACCAAGCGAA AAGTCGGCAT GCCTTACGCC GGTTGTGTTT CTTTATCGCA AATTTTCACT 
CGTCCGGACA AACTCGCTTT GAGTCAACAG CATTTCAACA AGGAAGGTCT CGGAAATCAA 
GCTCAGGTCG TGTGGCCTCA GTCACCGACT AGCAGCTCTC AAATGGGTAA TCGTCTGCAG 
AATTACCCCG AGAGCCAGCA GATGGTCCGA TATCAAAACC AGGAGCAACA GAATCGAATC 
GTTAACGGTT CGCCCATGGT GGTCGGTGAT AACAGTACCA TGATGGTACC CCAACAACAG 
TCCAACATAT CGCAACAGAT CCACCAGAAC CACAATGGTC AAAATCCCAG CGTTCCTCAG 
CAGCACATTA TCGGGCCCAA CGGCCAAGTG ATAAGTCTCC AAGGGGCCCT TAACTCGAAT 
CAGGTCAATA ATGGAGTGGT CATACAGGGT CAAAACGTCG TTCCTAATCC AAATCAATCC 
CGAGTTTTCA TCAACAGCCA ACCAAGCGAA CCCAGTGGAC CAGGCCGGCC CAACATCCAG 
ATGATTCCCG TAGGAGTCAC GAATCAAAGA GATGCCCAAA ACCGCCTCCA CCACTTCTAC 
AACAACCAAC AATACCCACA GATACAATAC GACGCGTCGA AGCAAATGCG ACCGACATTT 
ATGCCCGGCC ACAACCAAAT GAACGGTTTT CGCCCGCAAA ATAATCAGAT TATGCAAGCG 
CCGGTTATGA TTAACAACCA TCAAAAGATG CCATGGCCTA ATCGCATTCA AAATTCGCCC 
AATGCCTACG AAAGAGTGCC GCCGCTGCAT CCACACACAC CATCGCCCAC TATGTGGCAA 
GACGAGGTCA AACGGAAAAA GGTCAAATTG GGGAAAATCG TTAAAAATCG GCCGTATCAT 
ATGATGGATT CGGCGTTGCA TGCACCCTGT CCCAACATTG ACGTGCGGCA AATACCAGCC 
GAAAACGGCC GACAGGTTAT TATCAACCAG AATTCGGCCA GTCCCTCGTT TATGGAGGAC 
CCCAGTGGGT ATTTAGCCCA GCAGACGGCC CTGCTCAATA ATACCATCAA CAGACAAACA 
GGGGTGAACG CGTGCGCGGG CATGGTGTGC AGCAGCCCGC AGCAGCCCCA CCTAACACCA 
CCGAATTCGC AAACAAACGA GGTGCCTTTA CCCAGCAACG TGGTGATATC TCATATGAAA 
CAAACGCAAA ACATTACAAA TCGAACCAAT CAAACGTTAC ACGTAGTAAA AAGTCAAGTT 
CCTCAAGTAA TGTCCCAGCA GTATAATCAA ATGATGGCGG CTCACGCCGA CAGTAGCGTC 
ACCAACGACC AATGTCAAAA TTGCGACAGT CATAAAGGCC ATTCGAGACC TAACAGTCAA 
CCTGGAACTC CGAGTTCGTC GGCTATTGAC GATCCGGTGA CATCATCGAC TTATTCCGAC 
AAACAAAATA ATCAATCACC GGATTCAAGA CCGATTCAGG GCGGTACTGT TAGTACTAGC 
AATGTATCGC CATTGGATGG ATCACAATCA GATCCACCTA CACCTAACCC ACACACTCCA 
ACGCCTCCTA GGAATGATAA TATGCCATAT GCTGTCTACC AATCGCGTGA GGTTTGCAGT 
ACGAATTACC CACCGAAAAG TGACTGTTGT TCGAAAATGA ACACGCCGTA TATTTCGAGT 
ATAGTTACGA CGATGGCAAG TGGACGAACA GTTGGTAGTA ATACCATAAC GTCGGTTTTG 
GCCGGTCGTG CCAACACTGC CACAGTTTCA GTCAACAGTC CTGCTAACCT CCCAGCATCC 
ACAACCCCAA CAACAGTCAC ACAAACCGTT TCAAAATCCC CACTTGAAAT GGTCCAAAGC 
GTCGTCAGTA GCATACAAGT CCCTCAGTCT CAAACCAATC CCCAAATCTC TCCACCACAT 
ATCGTCAAAC ATTCCCCCAC CGGGTTACCT CCTGGCCATA TCCTTGTCTC AAGCGGCGGC 
CAGTTAATAA TGGCAAACAC CGGAAACAGC CAATCGGGCG TTATGCCACC CCCACCCCCC 
AAACTAGTCT CCAATCAAAC CGCAATGCCG CCAATGTCCG TCTCACCAAT GATCACAAAT 
GTCACTGCAG CGGTCAGTCA GGTCATACCG GCAGTAGCTC AGCAAGTTTT AGGCCAACAA 
ACCGTTTTAG TGAACGCCTT ACCCACACCT TTTGTCTTAC AAGGCGGAGT CACAATGACC 
ATGGACGGGT CCATGGTCGG CCAAAACATG CAATTACCTC AAATCGTCGC CGGAAATGTG 
ATACAACAAC AAATCCAACT GGATAACAGT GACCCACGAC GTACCACCGC GCTACTCTCA 
CCCGAAACCA AGAAAAAAGG AAAGAAACGA AAAATGTCAC AAACAGTGGC CAACATGCTT 
CACATAGCTG CCCAGCAAAA CTCCGGCGTT GTGATGTCAC AACAAGGCTT CCCGCAGCAA 
ATTCAAATGG CTCATAGTCC GCAAGGTCTA GCCACGGGCC CAGTGATGCA AGCCTTGACC 
ATAGTCCCCA GCAAAACAGG GGGACCCCCA CAGATCGTAA TGAACGGGCA ACCCATGACC 
AACACCAGTC CCCAACTCAT TGCAAACTCG CAACCGGCGC AACAAATCAA CCTCCTTCAG 
CCGGTGAATC TCATCAATGG CACCACCGGA GTGGTGCAAA ATTTCCCCAC GATTCAACAA 
TTCATAGTCC CGAATTTAGG CGGCATGGTT ATGAACCCGG ACGGCACCGC CACTATATTA 
CAGGACACTT CAAATATCGG CATGCAGTTA CAACTACAGA ACGTTAACGG ACAAAACGTC 
CTGACTCCGG TCCAAAACAA CGGAATGTTC AACGGGGGTC AGAGTATTCT AGCAGCGAGT 
CCGGCCGGCA TGGTCATTAG GGCCCCCACC TCGCAGGGGA AATTAATCCA GCAGCAGCAG 
CACAGTCCAG GTGCCCAGTT TTTGTCACCC AACGGGGGCC AGTTTGTCGT CAATGGGACA 
CAATTCAGCG GCCAGTTGAG CCCGCTTGTG GCTAGTGTGA GTCCGAGTCA GCAAGTTACC 
TTTGGGACGT CCACGCCGCA GATTAGACCA ACTCAGGGTC AACAAGAATT TATACAGTGT 
GGTCAAATGG GCCAGACTTT GATGGTTCCG TGTGCACCAA CGGTCGCCGT TTCCTCCTCG 
CAAAACACGA CTTTTGTCCA GCAAAACACT ACGATTGTTC AGCAGCAAAC CACGATGGTT 
TCGAATAGTC AACAGTTACA GAATTTCCAG AATAATGGAG GGAATCGAAC GACTGTGAAT 
GTGGATCAGA ATTTTATCAT CGCGAATGAT AATAAGCAAG TGCAGGCGCT TGTTCAGGTT 
CAGAGGGAGA GTCCCGGGAC GAATTATAGA CAGTCGGTGT CGACGCAAAC AGCGGTTAAT 
CAGAATGCAC AGACAGTGAC CACGTTTTGT CAGACGTCCA CGCTGTGTGC GGGGAGTCCC 
CCGGATACTA CAACGCATAG TCCGTTGGCG TCGGGAGGAC AGAGTCCTGC AACGGCCGAT 
ACGACCACAC ATACGGGAAG TACAGACGAT GGGCTGTCGC CAGCGCCGTC GAATTGTTCG 
GTGACGTGCG ATAGTGCAGG GAGACAGCCG GCTTGCACGA TGGCGATGGT GCACTGCATA 
TCCAGCAGCG AGCCCGATTC GGCTGATCTG AACACCCAAG GCTCTGACCA TGACTGGTCC 
CGAGCGTCGT CTATCCCTCA GCAAAAAAGC GACTTGTCGG AGATACATTT CCCCAAAAAA 
CAAAACACTC CGACCCACGT GGCTTACGCC GAATCGTCGA CAATGATGGC CGGTTTGCAT 
TTTATCGGCG ATCAAATGAA GGCCCAAGAG GCCGTCGTCA GTTCTCACAA TTTCGATAAA 
ATCGTACAGA AACGCAAAAG CAGCGATACT TTGCTCGTCA TGGAAACCCA TCACATGCAT 
TCGTCGCTTT TCGACGAGGA GGAAGATAAT GAGAAACGTG AAAAGTCAGA ACATGACTTT 
GTTGTTGGCG ATTTGGTTTG GGGGCCGGCT AAAGCAAGTC CAGCTTGGCC TGGCAAAATA 
ATACAAGTCG ACGGGAACGA TGTTTCGGTT AAATGGTTCG GCAGCGAAAA ATTAATTACA 
AAATTAGACA GAGGTTCCTT GCAGACACTC ACTGAAGGTC TCGATGCTCA CCACCAAGCG 
CGCAAAAAAT GCAGAACATC TCGCAAACTG AATTCTCATC TGGAGAAGGC CATCCAAGAG 
GCCATGGCTG AGCTGGATAA GGACTTGGAG CAAGAGACGA AGGAGGTGAA GCAGCAGAAG 
AAGAAAGCGC ACCGAGTGAG AAGCGGTCAT CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008201052.1
CDS	390..5585
Translation	MAGKPELSPAPGVQNVVVYVSNDNFAQAYAVGGQNAPQYGPPKPYPEKRPDYPEGYFVSSNGYGYEERPAKECDYDPAYVTVMGAEGPPQSVRCDSVRSETAESSCSSLSSADEGMVLVQNNQPEMVVYDPSVSVRPGVVFVGAGVHHHPNVAATAAANLPAAQQPSITVPYGWKRILNNGSIMYISPSNTALSSPEQVKEYLLTSGTCKCGLECHFKYDNTFNFDPKVASKPWTLSPDTNTGDLTKLCNHKRKIIAMASLECKPPDPAAKMRKDCNNKKTKRKVGMPYAGCVSLSQIFTRPDKLALSQQHFNKEGLGNQAQVVWPQSPTSSSQMGNRLQNYPESQQMVRYQNQEQQNRIVNGSPMVVGDNSTMMVPQQQSNISQQIHQNHNGQNPSVPQQHIIGPNGQVISLQGALNSNQVNNGVVIQGQNVVPNPNQSRVFINSQPSEPSGPGRPNIQMIPVGVTNQRDAQNRLHHFYNNQQYPQIQYDASKQMRPTFMPGHNQMNGFRPQNNQIMQAPVMINNHQKMPWPNRIQNSPNAYERVPPLHPHTPSPTMWQDEVKRKKVKLGKIVKNRPYHMMDSALHAPCPNIDVRQIPAENGRQVIINQNSASPSFMEDPSGYLAQQTALLNNTINRQTGVNACAGMVCSSPQQPHLTPPNSQTNEVPLPSNVVISHMKQTQNITNRTNQTLHVVKSQVPQVMSQQYNQMMAAHADSSVTNDQCQNCDSHKGHSRPNSQPGTPSSSAIDDPVTSSTYSDKQNNQSPDSRPIQGGTVSTSNVSPLDGSQSDPPTPNPHTPTPPRNDNMPYAVYQSREVCSTNYPPKSDCCSKMNTPYISSIVTTMASGRTVGSNTITSVLAGRANTATVSVNSPANLPASTTPTTVTQTVSKSPLEMVQSVVSSIQVPQSQTNPQISPPHIVKHSPTGLPPGHILVSSGGQLIMANTGNSQSGVMPPPPPKLVSNQTAMPPMSVSPMITNVTAAVSQVIPAVAQQVLGQQTVLVNALPTPFVLQGGVTMTMDGSMVGQNMQLPQIVAGNVIQQQIQLDNSDPRRTTALLSPETKKKGKKRKMSQTVANMLHIAAQQNSGVVMSQQGFPQQIQMAHSPQGLATGPVMQALTIVPSKTGGPPQIVMNGQPMTNTSPQLIANSQPAQQINLLQPVNLINGTTGVVQNFPTIQQFIVPNLGGMVMNPDGTATILQDTSNIGMQLQLQNVNGQNVLTPVQNNGMFNGGQSILAASPAGMVIRAPTSQGKLIQQQQHSPGAQFLSPNGGQFVVNGTQFSGQLSPLVASVSPSQQVTFGTSTPQIRPTQGQQEFIQCGQMGQTLMVPCAPTVAVSSSQNTTFVQQNTTIVQQQTTMVSNSQQLQNFQNNGGNRTTVNVDQNFIIANDNKQVQALVQVQRESPGTNYRQSVSTQTAVNQNAQTVTTFCQTSTLCAGSPPDTTTHSPLASGGQSPATADTTTHTGSTDDGLSPAPSNCSVTCDSAGRQPACTMAMVHCISSSEPDSADLNTQGSDHDWSRASSIPQQKSDLSEIHFPKKQNTPTHVAYAESSTMMAGLHFIGDQMKAQEAVVSSHNFDKIVQKRKSSDTLLVMETHHMHSSLFDEEEDNEKREKSEHDFVVGDLVWGPAKASPAWPGKIIQVDGNDVSVKWFGSEKLITKLDRGSLQTLTEGLDAHHQARKKCRTSRKLNSHLEKAIQEAMAELDKDLEQETKEVKQQKKKAHRVRSGHR

Target ORF information:

RefSeq Version	XM_008202830.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008202830.2

ORF Insert Sequence:

ATGGCAGGAA AGCCTGAGCT GTCGCCCGCC CCCGGCGTCC AGAATGTGGT AGTGTACGTT 
TCGAACGACA ACTTCGCCCA AGCGTACGCC GTCGGGGGGC AGAATGCGCC CCAGTACGGC 
CCCCCGAAGC CCTACCCCGA GAAGCGGCCC GACTACCCCG AGGGCTACTT CGTCTCGTCC 
AACGGCTACG GATACGAGGA GCGCCCGGCC AAGGAGTGCG ACTACGACCC GGCCTATGTG 
ACGGTCATGG GGGCCGAGGG GCCCCCGCAG AGTGTGCGGT GTGATTCAGT GCGGAGCGAG 
ACGGCCGAGT CGAGCTGCAG CAGCTTGAGC TCGGCCGACG AGGGGATGGT GCTAGTACAG 
AACAATCAAC CAGAGATGGT GGTGTATGAT CCCAGTGTTA GTGTTAGACC CGGTGTCGTG 
TTTGTGGGGG CCGGCGTCCA CCACCACCCC AATGTCGCCG CCACCGCCGC CGCCAACCTA 
CCCGCCGCCC AACAGCCCTC CATCACTGTG CCCTACGGCT GGAAGAGGAT TCTCAACAAC 
GGATCCATCA TGTACATAAG CCCAAGTAAC ACAGCTTTAA GTTCTCCGGA ACAAGTCAAA 
GAATATCTCC TCACGTCGGG CACTTGCAAG TGTGGATTAG AATGTCATTT CAAATATGAC 
AACACTTTTA ATTTCGATCC TAAGGTGGCC AGTAAACCGT GGACGCTATC ACCGGACACC 
AATACAGGGG ACCTGACCAA ATTGTGCAAC CACAAGAGAA AAATCATCGC CATGGCCTCG 
TTGGAGTGCA AGCCCCCGGA CCCCGCCGCC AAAATGCGAA AAGACTGCAA CAATAAGAAA 
ACCAAGCGAA AAGTCGGCAT GCCTTACGCC GGTTGTGTTT CTTTATCGCA AATTTTCACT 
CGTCCGGACA AACTCGCTTT GAGTCAACAG CATTTCAACA AGGAAGGTCT CGGAAATCAA 
GCTCAGGTCG TGTGGCCTCA GTCACCGACT AGCAGCTCTC AAATGGGTAA TCGTCTGCAG 
AATTACCCCG AGAGCCAGCA GATGGTCCGA TATCAAAACC AGGAGCAACA GAATCGAATC 
GTTAACGGTT CGCCCATGGT GGTCGGTGAT AACAGTACCA TGATGGTACC CCAACAACAG 
TCCAACATAT CGCAACAGAT CCACCAGAAC CACAATGGTC AAAATCCCAG CGTTCCTCAG 
CAGCACATTA TCGGGCCCAA CGGCCAAGTG ATAAGTCTCC AAGGGGCCCT TAACTCGAAT 
CAGGTCAATA ATGGAGTGGT CATACAGGGT CAAAACGTCG TTCCTAATCC AAATCAATCC 
CGAGTTTTCA TCAACAGCCA ACCAAGCGAA CCCAGTGGAC CAGGCCGGCC CAACATCCAG 
ATGATTCCCG TAGGAGTCAC GAATCAAAGA GATGCCCAAA ACCGCCTCCA CCACTTCTAC 
AACAACCAAC AATACCCACA GATACAATAC GACGCGTCGA AGCAAATGCG ACCGACATTT 
ATGCCCGGCC ACAACCAAAT GAACGGTTTT CGCCCGCAAA ATAATCAGAT TATGCAAGCG 
CCGGTTATGA TTAACAACCA TCAAAAGATG CCATGGCCTA ATCGCATTCA AAATTCGCCC 
AATGCCTACG AAAGAGTGCC GCCGCTGCAT CCACACACAC CATCGCCCAC TATGTGGCAA 
GACGAGGTCA AACGGAAAAA GGTCAAATTG GGGAAAATCG TTAAAAATCG GCCGTATCAT 
ATGATGGATT CGGCGTTGCA TGCACCCTGT CCCAACATTG ACGTGCGGCA AATACCAGCC 
GAAAACGGCC GACAGGTTAT TATCAACCAG AATTCGGCCA GTCCCTCGTT TATGGAGGAC 
CCCAGTGGGT ATTTAGCCCA GCAGACGGCC CTGCTCAATA ATACCATCAA CAGACAAACA 
GGGGTGAACG CGTGCGCGGG CATGGTGTGC AGCAGCCCGC AGCAGCCCCA CCTAACACCA 
CCGAATTCGC AAACAAACGA GGTGCCTTTA CCCAGCAACG TGGTGATATC TCATATGAAA 
CAAACGCAAA ACATTACAAA TCGAACCAAT CAAACGTTAC ACGTAGTAAA AAGTCAAGTT 
CCTCAAGTAA TGTCCCAGCA GTATAATCAA ATGATGGCGG CTCACGCCGA CAGTAGCGTC 
ACCAACGACC AATGTCAAAA TTGCGACAGT CATAAAGGCC ATTCGAGACC TAACAGTCAA 
CCTGGAACTC CGAGTTCGTC GGCTATTGAC GATCCGGTGA CATCATCGAC TTATTCCGAC 
AAACAAAATA ATCAATCACC GGATTCAAGA CCGATTCAGG GCGGTACTGT TAGTACTAGC 
AATGTATCGC CATTGGATGG ATCACAATCA GATCCACCTA CACCTAACCC ACACACTCCA 
ACGCCTCCTA GGAATGATAA TATGCCATAT GCTGTCTACC AATCGCGTGA GGTTTGCAGT 
ACGAATTACC CACCGAAAAG TGACTGTTGT TCGAAAATGA ACACGCCGTA TATTTCGAGT 
ATAGTTACGA CGATGGCAAG TGGACGAACA GTTGGTAGTA ATACCATAAC GTCGGTTTTG 
GCCGGTCGTG CCAACACTGC CACAGTTTCA GTCAACAGTC CTGCTAACCT CCCAGCATCC 
ACAACCCCAA CAACAGTCAC ACAAACCGTT TCAAAATCCC CACTTGAAAT GGTCCAAAGC 
GTCGTCAGTA GCATACAAGT CCCTCAGTCT CAAACCAATC CCCAAATCTC TCCACCACAT 
ATCGTCAAAC ATTCCCCCAC CGGGTTACCT CCTGGCCATA TCCTTGTCTC AAGCGGCGGC 
CAGTTAATAA TGGCAAACAC CGGAAACAGC CAATCGGGCG TTATGCCACC CCCACCCCCC 
AAACTAGTCT CCAATCAAAC CGCAATGCCG CCAATGTCCG TCTCACCAAT GATCACAAAT 
GTCACTGCAG CGGTCAGTCA GGTCATACCG GCAGTAGCTC AGCAAGTTTT AGGCCAACAA 
ACCGTTTTAG TGAACGCCTT ACCCACACCT TTTGTCTTAC AAGGCGGAGT CACAATGACC 
ATGGACGGGT CCATGGTCGG CCAAAACATG CAATTACCTC AAATCGTCGC CGGAAATGTG 
ATACAACAAC AAATCCAACT GGATAACAGT GACCCACGAC GTACCACCGC GCTACTCTCA 
CCCGAAACCA AGAAAAAAGG AAAGAAACGA AAAATGTCAC AAACAGTGGC CAACATGCTT 
CACATAGCTG CCCAGCAAAA CTCCGGCGTT GTGATGTCAC AACAAGGCTT CCCGCAGCAA 
ATTCAAATGG CTCATAGTCC GCAAGGTCTA GCCACGGGCC CAGTGATGCA AGCCTTGACC 
ATAGTCCCCA GCAAAACAGG GGGACCCCCA CAGATCGTAA TGAACGGGCA ACCCATGACC 
AACACCAGTC CCCAACTCAT TGCAAACTCG CAACCGGCGC AACAAATCAA CCTCCTTCAG 
CCGGTGAATC TCATCAATGG CACCACCGGA GTGGTGCAAA ATTTCCCCAC GATTCAACAA 
TTCATAGTCC CGAATTTAGG CGGCATGGTT ATGAACCCGG ACGGCACCGC CACTATATTA 
CAGGACACTT CAAATATCGG CATGCAGTTA CAACTACAGA ACGTTAACGG ACAAAACGTC 
CTGACTCCGG TCCAAAACAA CGGAATGTTC AACGGGGGTC AGAGTATTCT AGCAGCGAGT 
CCGGCCGGCA TGGTCATTAG GGCCCCCACC TCGCAGGGGA AATTAATCCA GCAGCAGCAG 
CACAGTCCAG GTGCCCAGTT TTTGTCACCC AACGGGGGCC AGTTTGTCGT CAATGGGACA 
CAATTCAGCG GCCAGTTGAG CCCGCTTGTG GCTAGTGTGA GTCCGAGTCA GCAAGTTACC 
TTTGGGACGT CCACGCCGCA GATTAGACCA ACTCAGGGTC AACAAGAATT TATACAGTGT 
GGTCAAATGG GCCAGACTTT GATGGTTCCG TGTGCACCAA CGGTCGCCGT TTCCTCCTCG 
CAAAACACGA CTTTTGTCCA GCAAAACACT ACGATTGTTC AGCAGCAAAC CACGATGGTT 
TCGAATAGTC AACAGTTACA GAATTTCCAG AATAATGGAG GGAATCGAAC GACTGTGAAT 
GTGGATCAGA ATTTTATCAT CGCGAATGAT AATAAGCAAG TGCAGGCGCT TGTTCAGGTT 
CAGAGGGAGA GTCCCGGGAC GAATTATAGA CAGTCGGTGT CGACGCAAAC AGCGGTTAAT 
CAGAATGCAC AGACAGTGAC CACGTTTTGT CAGACGTCCA CGCTGTGTGC GGGGAGTCCC 
CCGGATACTA CAACGCATAG TCCGTTGGCG TCGGGAGGAC AGAGTCCTGC AACGGCCGAT 
ACGACCACAC ATACGGGAAG TACAGACGAT GGGCTGTCGC CAGCGCCGTC GAATTGTTCG 
GTGACGTGCG ATAGTGCAGG GAGACAGCCG GCTTGCACGA TGGCGATGGT GCACTGCATA 
TCCAGCAGCG AGCCCGATTC GGCTGATCTG AACACCCAAG GCTCTGACCA TGACTGGTCC 
CGAGCGTCGT CTATCCCTCA GCAAAAAAGC GACTTGTCGG AGATACATTT CCCCAAAAAA 
CAAAACACTC CGACCCACGT GGCTTACGCC GAATCGTCGA CAATGATGGC CGGTTTGCAT 
TTTATCGGCG ATCAAATGAA GGCCCAAGAG GCCGTCGTCA GTTCTCACAA TTTCGATAAA 
ATCGTACAGA AACGCAAAAG CAGCGATACT TTGCTCGTCA TGGAAACCCA TCACATGCAT 
TCGTCGCTTT TCGACGAGGA GGAAGATAAT GAGAAACGTG AAAAGTCAGA ACATGACTTT 
GTTGTTGGCG ATTTGGTTTG GGGGCCGGCT AAAGCAAGTC CAGCTTGGCC TGGCAAAATA 
ATACAAGTCG ACGGGAACGA TGTTTCGGTT AAATGGTTCG GCAGCGAAAA ATTAATTACA 
AAATTAGACA GAGGTTCCTT GCAGACACTC ACTGAAGGTC TCGATGCTCA CCACCAAGCG 
CGCAAAAAAT GCAGAACATC TCGCAAACTG AATTCTCATC TGGAGAAGGC CATCCAAGAG 
GCCATGGCTG AGCTGGATAA GGACTTGGAG CAAGAGACGA AGGAGGTGAA GCAGCAGAAG 
AAGAAAGCGC ACCGAGTGAG AAGCGGTCAT CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC660029 ( XM_966293.3 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_971386.1 Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X3, mRNA.

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CloneID	OTg01697
Clone ID Related Accession (Same CDS sequence)	XM_966293.3
Accession Version	XM_966293.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	methyl-CpG-binding domain protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450135.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_966293.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA AGCCTGAGCT GTCGCCCGCC CCCGGCGTCC AGAATGTGGT AGTGTACGTT 
TCGAACGACA ACTTCGCCCA AGCGTACGCC GTCGGGGGGC AGAATGCGCC CCAGTACGGC 
CCCCCGAAGC CCTACCCCGA GAAGCGGCCC GACTACCCCG AGGGCTACTT CGTCTCGTCC 
AACGGCTACG GATACGAGGA GCGCCCGGCC AAGGAGTGCG ACTACGACCC GGCCTATGTG 
ACGGTCATGG GGGCCGAGGG GCCCCCGCAG AGTGTGCGGT GTGATTCAGT GCGGAGCGAG 
ACGGCCGAGT CGAGCTGCAG CAGCTTGAGC TCGGCCGACG AGGGGATGGT GCTAGTACAG 
AACAATCAAC CAGAGATGGT GGTGTATGAT CCCAGTGTTA GTGTTAGACC CGGTGTCGTG 
TTTGTGGGGG CCGGCGTCCA CCACCACCCC AATGTCGCCG CCACCGCCGC CGCCAACCTA 
CCCGCCGCCC AACAGCCCTC CATCACTGTG CCCTACGGCT GGAAGAGGAT TCTCAACAAC 
GGATCCATCA TGTACATAAG CCCAAGTAAC ACAGCTTTAA GTTCTCCGGA ACAAGTCAAA 
GAATATCTCC TCACGTCGGG CACTTGCAAG TGTGGATTAG AATGTCATTT CAAATATGAC 
AACACTTTTA ATTTCGATCC TAAGGTGGCC AGTAAACCGT GGACGCTATC ACCGGACACC 
AATACAGGGG ACCTGACCAA ATTGTGCAAC CACAAGAGAA AAATCATCGC CATGGCCTCG 
TTGGAGTGCA AGCCCCCGGA CCCCGCCGCC AAAATGCGAA AAGACTGCAA CAATAAGAAA 
ACCAAGCGAA AAGTCGGCAT GCCTTACGCC GGTTGTGTTT CTTTATCGCA AATTTTCACT 
CGTCCGGACA AACTCGCTTT GAGTCAACAG CATTTCAACA AGGAAGGTCT CGGAAATCAA 
GCTCAGGTGT GGCCTCAGTC ACCGACTAGC AGCTCTCAAA TGGGTAATCG TCTGCAGAAT 
TACCCCGAGA GCCAGCAGAT GGTCCGATAT CAAAACCAGG AGCAACAGAA TCGAATCGTT 
AACGGTTCGC CCATGGTGGT CGGTGATAAC AGTACCATGA TGGTACCCCA ACAACAGTCC 
AACATATCGC AACAGATCCA CCAGAACCAC AATGGTCAAA ATCCCAGCGT TCCTCAGCAG 
CACATTATCG GGCCCAACGG CCAAGTGATA AGTCTCCAAG GGGCCCTTAA CTCGAATCAG 
GTCAATAATG GAGTGGTCAT ACAGGGTCAA AACGTCGTTC CTAATCCAAA TCAATCCCGA 
GTTTTCATCA ACAGCCAACC AAGCGAACCC AGTGGACCAG GCCGGCCCAA CATCCAGATG 
ATTCCCGTAG GAGTCACGAA TCAAAGAGAT GCCCAAAACC GCCTCCACCA CTTCTACAAC 
AACCAACAAT ACCCACAGAT ACAATACGAC GCGTCGAAGC AAATGCGACC GACATTTATG 
CCCGGCCACA ACCAAATGAA CGGTTTTCGC CCGCAAAATA ATCAGATTAT GCAAGCGCCG 
GTTATGATTA ACAACCATCA AAAGATGCCA TGGCCTAATC GCATTCAAAA TTCGCCCAAT 
GCCTACGAAA GAGTGCCGCC GCTGCATCCA CACACACCAT CGCCCACTAT GTGGCAAGAC 
GAGGTCAAAC GGAAAAAGGT CAAATTGGGG AAAATCGTTA AAAATCGGCC GTATCATATG 
ATGGATTCGG CGTTGCATGC ACCCTGTCCC AACATTGACG TGCGGCAAAT ACCAGCCGAA 
AACGGCCGAC AGGTTATTAT CAACCAGAAT TCGGCCAGTC CCTCGTTTAT GGAGGACCCC 
AGTGGGTATT TAGCCCAGCA GACGGCCCTG CTCAATAATA CCATCAACAG ACAAACAGGG 
GTGAACGCGT GCGCGGGCAT GGTGTGCAGC AGCCCGCAGC AGCCCCACCT AACACCACCG 
AATTCGCAAA CAAACGAGGT GCCTTTACCC AGCAACGTGG TGATATCTCA TATGAAACAA 
ACGCAAAACA TTACAAATCG AACCAATCAA ACGTTACACG TAGTAAAAAG TCAAGTTCCT 
CAAGTAATGT CCCAGCAGTA TAATCAAATG ATGGCGGCTC ACGCCGACAG TAGCGTCACC 
AACGACCAAT GTCAAAATTG CGACAGTCAT AAAGGCCATT CGAGACCTAA CAGTCAACCT 
GGAACTCCGA GTTCGTCGGC TATTGACGAT CCGGTGACAT CATCGACTTA TTCCGACAAA 
CAAAATAATC AATCACCGGA TTCAAGACCG ATTCAGGGCG GTACTGTTAG TACTAGCAAT 
GTATCGCCAT TGGATGGATC ACAATCAGAT CCACCTACAC CTAACCCACA CACTCCAACG 
CCTCCTAGGA ATGATAATAT GCCATATGCT GTCTACCAAT CGCGTGAGGT TTGCAGTACG 
AATTACCCAC CGAAAAGTGA CTGTTGTTCG AAAATGAACA CGCCGTATAT TTCGAGTATA 
GTTACGACGA TGGCAAGTGG ACGAACAGTT GGTAGTAATA CCATAACGTC GGTTTTGGCC 
GGTCGTGCCA ACACTGCCAC AGTTTCAGTC AACAGTCCTG CTAACCTCCC AGCATCCACA 
ACCCCAACAA CAGTCACACA AACCGTTTCA AAATCCCCAC TTGAAATGGT CCAAAGCGTC 
GTCAGTAGCA TACAAGTCCC TCAGTCTCAA ACCAATCCCC AAATCTCTCC ACCACATATC 
GTCAAACATT CCCCCACCGG GTTACCTCCT GGCCATATCC TTGTCTCAAG CGGCGGCCAG 
TTAATAATGG CAAACACCGG AAACAGCCAA TCGGGCGTTA TGCCACCCCC ACCCCCCAAA 
CTAGTCTCCA ATCAAACCGC AATGCCGCCA ATGTCCGTCT CACCAATGAT CACAAATGTC 
ACTGCAGCGG TCAGTCAGGT CATACCGGCA GTAGCTCAGC AAGTTTTAGG CCAACAAACC 
GTTTTAGTGA ACGCCTTACC CACACCTTTT GTCTTACAAG GCGGAGTCAC AATGACCATG 
GACGGGTCCA TGGTCGGCCA AAACATGCAA TTACCTCAAA TCGTCGCCGG AAATGTGATA 
CAACAACAAA TCCAACTGGA TAACAGTGAC CCACGACGTA CCACCGCGCT ACTCTCACCC 
GAAACCAAGA AAAAAGGAAA GAAACGAAAA ATGTCACAAA CAGTGGCCAA CATGCTTCAC 
ATAGCTGCCC AGCAAAACTC CGGCGTTGTG ATGTCACAAC AAGGCTTCCC GCAGCAAATT 
CAAATGGCTC ATAGTCCGCA AGGTCTAGCC ACGGGCCCAG TGATGCAAGC CTTGACCATA 
GTCCCCAGCA AAACAGGGGG ACCCCCACAG ATCGTAATGA ACGGGCAACC CATGACCAAC 
ACCAGTCCCC AACTCATTGC AAACTCGCAA CCGGCGCAAC AAATCAACCT CCTTCAGCCG 
GTGAATCTCA TCAATGGCAC CACCGGAGTG GTGCAAAATT TCCCCACGAT TCAACAATTC 
ATAGTCCCGA ATTTAGGCGG CATGGTTATG AACCCGGACG GCACCGCCAC TATATTACAG 
GACACTTCAA ATATCGGCAT GCAGTTACAA CTACAGAACG TTAACGGACA AAACGTCCTG 
ACTCCGGTCC AAAACAACGG AATGTTCAAC GGGGGTCAGA GTATTCTAGC AGCGAGTCCG 
GCCGGCATGG TCATTAGGGC CCCCACCTCG CAGGGGAAAT TAATCCAGCA GCAGCAGCAC 
AGTCCAGGTG CCCAGTTTTT GTCACCCAAC GGGGGCCAGT TTGTCGTCAA TGGGACACAA 
TTCAGCGGCC AGTTGAGCCC GCTTGTGGCT AGTGTGAGTC CGAGTCAGCA AGTTACCTTT 
GGGACGTCCA CGCCGCAGAT TAGACCAACT CAGGGTCAAC AAGAATTTAT ACAGTGTGGT 
CAAATGGGCC AGACTTTGAT GGTTCCGTGT GCACCAACGG TCGCCGTTTC CTCCTCGCAA 
AACACGACTT TTGTCCAGCA AAACACTACG ATTGTTCAGC AGCAAACCAC GATGGTTTCG 
AATAGTCAAC AGTTACAGAA TTTCCAGAAT AATGGAGGGA ATCGAACGAC TGTGAATGTG 
GATCAGAATT TTATCATCGC GAATGATAAT AAGCAAGTGC AGGCGCTTGT TCAGGTTCAG 
AGGGAGAGTC CCGGGACGAA TTATAGACAG TCGGTGTCGA CGCAAACAGC GGTTAATCAG 
AATGCACAGA CAGTGACCAC GTTTTGTCAG ACGTCCACGC TGTGTGCGGG GAGTCCCCCG 
GATACTACAA CGCATAGTCC GTTGGCGTCG GGAGGACAGA GTCCTGCAAC GGCCGATACG 
ACCACACATA CGGGAAGTAC AGACGATGGG CTGTCGCCAG CGCCGTCGAA TTGTTCGGTG 
ACGTGCGATA GTGCAGGGAG ACAGCCGGCT TGCACGATGG CGATGGTGCA CTGCATATCC 
AGCAGCGAGC CCGATTCGGC TGATCTGAAC ACCCAAGGCT CTGACCATGA CTGGTCCCGA 
GCGTCGTCTA TCCCTCAGCA AAAAAGCGAC TTGTCGGAGA TACATTTCCC CAAAAAACAA 
AACACTCCGA CCCACGTGGC TTACGCCGAA TCGTCGACAA TGATGGCCGG TTTGCATTTT 
ATCGGCGATC AAATGAAGGC CCAAGAGGCC GTCGTCAGTT CTCACAATTT CGATAAAATC 
GTACAGAAAC GCAAAAGCAG CGATACTTTG CTCGTCATGG AAACCCATCA CATGCATTCG 
TCGCTTTTCG ACGAGGAGGA AGATAATGAG AAACGTGAAA AGTCAGAACA TGACTTTGTT 
GTTGGCGATT TGGTTTGGGG GCCGGCTAAA GCAAGTCCAG CTTGGCCTGG CAAAATAATA 
CAAGTCGACG GGAACGATGT TTCGGTTAAA TGGTTCGGCA GCGAAAAATT AATTACAAAA 
TTAGACAGAG GTTCCTTGCA GACACTCACT GAAGGTCTCG ATGCTCACCA CCAAGCGCGC 
AAAAAATGCA GAACATCTCG CAAACTGAAT TCTCATCTGG AGAAGGCCAT CCAAGAGGCC 
ATGGCTGAGC TGGATAAGGA CTTGGAGCAA GAGACGAAGG AGGTGAAGCA GCAGAAGAAG 
AAAGCGCACC GAGTGAGAAG CGGTCATCGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_971386.1
CDS	388..5580
Translation	MAGKPELSPAPGVQNVVVYVSNDNFAQAYAVGGQNAPQYGPPKPYPEKRPDYPEGYFVSSNGYGYEERPAKECDYDPAYVTVMGAEGPPQSVRCDSVRSETAESSCSSLSSADEGMVLVQNNQPEMVVYDPSVSVRPGVVFVGAGVHHHPNVAATAAANLPAAQQPSITVPYGWKRILNNGSIMYISPSNTALSSPEQVKEYLLTSGTCKCGLECHFKYDNTFNFDPKVASKPWTLSPDTNTGDLTKLCNHKRKIIAMASLECKPPDPAAKMRKDCNNKKTKRKVGMPYAGCVSLSQIFTRPDKLALSQQHFNKEGLGNQAQVWPQSPTSSSQMGNRLQNYPESQQMVRYQNQEQQNRIVNGSPMVVGDNSTMMVPQQQSNISQQIHQNHNGQNPSVPQQHIIGPNGQVISLQGALNSNQVNNGVVIQGQNVVPNPNQSRVFINSQPSEPSGPGRPNIQMIPVGVTNQRDAQNRLHHFYNNQQYPQIQYDASKQMRPTFMPGHNQMNGFRPQNNQIMQAPVMINNHQKMPWPNRIQNSPNAYERVPPLHPHTPSPTMWQDEVKRKKVKLGKIVKNRPYHMMDSALHAPCPNIDVRQIPAENGRQVIINQNSASPSFMEDPSGYLAQQTALLNNTINRQTGVNACAGMVCSSPQQPHLTPPNSQTNEVPLPSNVVISHMKQTQNITNRTNQTLHVVKSQVPQVMSQQYNQMMAAHADSSVTNDQCQNCDSHKGHSRPNSQPGTPSSSAIDDPVTSSTYSDKQNNQSPDSRPIQGGTVSTSNVSPLDGSQSDPPTPNPHTPTPPRNDNMPYAVYQSREVCSTNYPPKSDCCSKMNTPYISSIVTTMASGRTVGSNTITSVLAGRANTATVSVNSPANLPASTTPTTVTQTVSKSPLEMVQSVVSSIQVPQSQTNPQISPPHIVKHSPTGLPPGHILVSSGGQLIMANTGNSQSGVMPPPPPKLVSNQTAMPPMSVSPMITNVTAAVSQVIPAVAQQVLGQQTVLVNALPTPFVLQGGVTMTMDGSMVGQNMQLPQIVAGNVIQQQIQLDNSDPRRTTALLSPETKKKGKKRKMSQTVANMLHIAAQQNSGVVMSQQGFPQQIQMAHSPQGLATGPVMQALTIVPSKTGGPPQIVMNGQPMTNTSPQLIANSQPAQQINLLQPVNLINGTTGVVQNFPTIQQFIVPNLGGMVMNPDGTATILQDTSNIGMQLQLQNVNGQNVLTPVQNNGMFNGGQSILAASPAGMVIRAPTSQGKLIQQQQHSPGAQFLSPNGGQFVVNGTQFSGQLSPLVASVSPSQQVTFGTSTPQIRPTQGQQEFIQCGQMGQTLMVPCAPTVAVSSSQNTTFVQQNTTIVQQQTTMVSNSQQLQNFQNNGGNRTTVNVDQNFIIANDNKQVQALVQVQRESPGTNYRQSVSTQTAVNQNAQTVTTFCQTSTLCAGSPPDTTTHSPLASGGQSPATADTTTHTGSTDDGLSPAPSNCSVTCDSAGRQPACTMAMVHCISSSEPDSADLNTQGSDHDWSRASSIPQQKSDLSEIHFPKKQNTPTHVAYAESSTMMAGLHFIGDQMKAQEAVVSSHNFDKIVQKRKSSDTLLVMETHHMHSSLFDEEEDNEKREKSEHDFVVGDLVWGPAKASPAWPGKIIQVDGNDVSVKWFGSEKLITKLDRGSLQTLTEGLDAHHQARKKCRTSRKLNSHLEKAIQEAMAELDKDLEQETKEVKQQKKKAHRVRSGHR

Target ORF information:

RefSeq Version	XM_966293.3
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_966293.3

ORF Insert Sequence:

ATGGCAGGAA AGCCTGAGCT GTCGCCCGCC CCCGGCGTCC AGAATGTGGT AGTGTACGTT 
TCGAACGACA ACTTCGCCCA AGCGTACGCC GTCGGGGGGC AGAATGCGCC CCAGTACGGC 
CCCCCGAAGC CCTACCCCGA GAAGCGGCCC GACTACCCCG AGGGCTACTT CGTCTCGTCC 
AACGGCTACG GATACGAGGA GCGCCCGGCC AAGGAGTGCG ACTACGACCC GGCCTATGTG 
ACGGTCATGG GGGCCGAGGG GCCCCCGCAG AGTGTGCGGT GTGATTCAGT GCGGAGCGAG 
ACGGCCGAGT CGAGCTGCAG CAGCTTGAGC TCGGCCGACG AGGGGATGGT GCTAGTACAG 
AACAATCAAC CAGAGATGGT GGTGTATGAT CCCAGTGTTA GTGTTAGACC CGGTGTCGTG 
TTTGTGGGGG CCGGCGTCCA CCACCACCCC AATGTCGCCG CCACCGCCGC CGCCAACCTA 
CCCGCCGCCC AACAGCCCTC CATCACTGTG CCCTACGGCT GGAAGAGGAT TCTCAACAAC 
GGATCCATCA TGTACATAAG CCCAAGTAAC ACAGCTTTAA GTTCTCCGGA ACAAGTCAAA 
GAATATCTCC TCACGTCGGG CACTTGCAAG TGTGGATTAG AATGTCATTT CAAATATGAC 
AACACTTTTA ATTTCGATCC TAAGGTGGCC AGTAAACCGT GGACGCTATC ACCGGACACC 
AATACAGGGG ACCTGACCAA ATTGTGCAAC CACAAGAGAA AAATCATCGC CATGGCCTCG 
TTGGAGTGCA AGCCCCCGGA CCCCGCCGCC AAAATGCGAA AAGACTGCAA CAATAAGAAA 
ACCAAGCGAA AAGTCGGCAT GCCTTACGCC GGTTGTGTTT CTTTATCGCA AATTTTCACT 
CGTCCGGACA AACTCGCTTT GAGTCAACAG CATTTCAACA AGGAAGGTCT CGGAAATCAA 
GCTCAGGTGT GGCCTCAGTC ACCGACTAGC AGCTCTCAAA TGGGTAATCG TCTGCAGAAT 
TACCCCGAGA GCCAGCAGAT GGTCCGATAT CAAAACCAGG AGCAACAGAA TCGAATCGTT 
AACGGTTCGC CCATGGTGGT CGGTGATAAC AGTACCATGA TGGTACCCCA ACAACAGTCC 
AACATATCGC AACAGATCCA CCAGAACCAC AATGGTCAAA ATCCCAGCGT TCCTCAGCAG 
CACATTATCG GGCCCAACGG CCAAGTGATA AGTCTCCAAG GGGCCCTTAA CTCGAATCAG 
GTCAATAATG GAGTGGTCAT ACAGGGTCAA AACGTCGTTC CTAATCCAAA TCAATCCCGA 
GTTTTCATCA ACAGCCAACC AAGCGAACCC AGTGGACCAG GCCGGCCCAA CATCCAGATG 
ATTCCCGTAG GAGTCACGAA TCAAAGAGAT GCCCAAAACC GCCTCCACCA CTTCTACAAC 
AACCAACAAT ACCCACAGAT ACAATACGAC GCGTCGAAGC AAATGCGACC GACATTTATG 
CCCGGCCACA ACCAAATGAA CGGTTTTCGC CCGCAAAATA ATCAGATTAT GCAAGCGCCG 
GTTATGATTA ACAACCATCA AAAGATGCCA TGGCCTAATC GCATTCAAAA TTCGCCCAAT 
GCCTACGAAA GAGTGCCGCC GCTGCATCCA CACACACCAT CGCCCACTAT GTGGCAAGAC 
GAGGTCAAAC GGAAAAAGGT CAAATTGGGG AAAATCGTTA AAAATCGGCC GTATCATATG 
ATGGATTCGG CGTTGCATGC ACCCTGTCCC AACATTGACG TGCGGCAAAT ACCAGCCGAA 
AACGGCCGAC AGGTTATTAT CAACCAGAAT TCGGCCAGTC CCTCGTTTAT GGAGGACCCC 
AGTGGGTATT TAGCCCAGCA GACGGCCCTG CTCAATAATA CCATCAACAG ACAAACAGGG 
GTGAACGCGT GCGCGGGCAT GGTGTGCAGC AGCCCGCAGC AGCCCCACCT AACACCACCG 
AATTCGCAAA CAAACGAGGT GCCTTTACCC AGCAACGTGG TGATATCTCA TATGAAACAA 
ACGCAAAACA TTACAAATCG AACCAATCAA ACGTTACACG TAGTAAAAAG TCAAGTTCCT 
CAAGTAATGT CCCAGCAGTA TAATCAAATG ATGGCGGCTC ACGCCGACAG TAGCGTCACC 
AACGACCAAT GTCAAAATTG CGACAGTCAT AAAGGCCATT CGAGACCTAA CAGTCAACCT 
GGAACTCCGA GTTCGTCGGC TATTGACGAT CCGGTGACAT CATCGACTTA TTCCGACAAA 
CAAAATAATC AATCACCGGA TTCAAGACCG ATTCAGGGCG GTACTGTTAG TACTAGCAAT 
GTATCGCCAT TGGATGGATC ACAATCAGAT CCACCTACAC CTAACCCACA CACTCCAACG 
CCTCCTAGGA ATGATAATAT GCCATATGCT GTCTACCAAT CGCGTGAGGT TTGCAGTACG 
AATTACCCAC CGAAAAGTGA CTGTTGTTCG AAAATGAACA CGCCGTATAT TTCGAGTATA 
GTTACGACGA TGGCAAGTGG ACGAACAGTT GGTAGTAATA CCATAACGTC GGTTTTGGCC 
GGTCGTGCCA ACACTGCCAC AGTTTCAGTC AACAGTCCTG CTAACCTCCC AGCATCCACA 
ACCCCAACAA CAGTCACACA AACCGTTTCA AAATCCCCAC TTGAAATGGT CCAAAGCGTC 
GTCAGTAGCA TACAAGTCCC TCAGTCTCAA ACCAATCCCC AAATCTCTCC ACCACATATC 
GTCAAACATT CCCCCACCGG GTTACCTCCT GGCCATATCC TTGTCTCAAG CGGCGGCCAG 
TTAATAATGG CAAACACCGG AAACAGCCAA TCGGGCGTTA TGCCACCCCC ACCCCCCAAA 
CTAGTCTCCA ATCAAACCGC AATGCCGCCA ATGTCCGTCT CACCAATGAT CACAAATGTC 
ACTGCAGCGG TCAGTCAGGT CATACCGGCA GTAGCTCAGC AAGTTTTAGG CCAACAAACC 
GTTTTAGTGA ACGCCTTACC CACACCTTTT GTCTTACAAG GCGGAGTCAC AATGACCATG 
GACGGGTCCA TGGTCGGCCA AAACATGCAA TTACCTCAAA TCGTCGCCGG AAATGTGATA 
CAACAACAAA TCCAACTGGA TAACAGTGAC CCACGACGTA CCACCGCGCT ACTCTCACCC 
GAAACCAAGA AAAAAGGAAA GAAACGAAAA ATGTCACAAA CAGTGGCCAA CATGCTTCAC 
ATAGCTGCCC AGCAAAACTC CGGCGTTGTG ATGTCACAAC AAGGCTTCCC GCAGCAAATT 
CAAATGGCTC ATAGTCCGCA AGGTCTAGCC ACGGGCCCAG TGATGCAAGC CTTGACCATA 
GTCCCCAGCA AAACAGGGGG ACCCCCACAG ATCGTAATGA ACGGGCAACC CATGACCAAC 
ACCAGTCCCC AACTCATTGC AAACTCGCAA CCGGCGCAAC AAATCAACCT CCTTCAGCCG 
GTGAATCTCA TCAATGGCAC CACCGGAGTG GTGCAAAATT TCCCCACGAT TCAACAATTC 
ATAGTCCCGA ATTTAGGCGG CATGGTTATG AACCCGGACG GCACCGCCAC TATATTACAG 
GACACTTCAA ATATCGGCAT GCAGTTACAA CTACAGAACG TTAACGGACA AAACGTCCTG 
ACTCCGGTCC AAAACAACGG AATGTTCAAC GGGGGTCAGA GTATTCTAGC AGCGAGTCCG 
GCCGGCATGG TCATTAGGGC CCCCACCTCG CAGGGGAAAT TAATCCAGCA GCAGCAGCAC 
AGTCCAGGTG CCCAGTTTTT GTCACCCAAC GGGGGCCAGT TTGTCGTCAA TGGGACACAA 
TTCAGCGGCC AGTTGAGCCC GCTTGTGGCT AGTGTGAGTC CGAGTCAGCA AGTTACCTTT 
GGGACGTCCA CGCCGCAGAT TAGACCAACT CAGGGTCAAC AAGAATTTAT ACAGTGTGGT 
CAAATGGGCC AGACTTTGAT GGTTCCGTGT GCACCAACGG TCGCCGTTTC CTCCTCGCAA 
AACACGACTT TTGTCCAGCA AAACACTACG ATTGTTCAGC AGCAAACCAC GATGGTTTCG 
AATAGTCAAC AGTTACAGAA TTTCCAGAAT AATGGAGGGA ATCGAACGAC TGTGAATGTG 
GATCAGAATT TTATCATCGC GAATGATAAT AAGCAAGTGC AGGCGCTTGT TCAGGTTCAG 
AGGGAGAGTC CCGGGACGAA TTATAGACAG TCGGTGTCGA CGCAAACAGC GGTTAATCAG 
AATGCACAGA CAGTGACCAC GTTTTGTCAG ACGTCCACGC TGTGTGCGGG GAGTCCCCCG 
GATACTACAA CGCATAGTCC GTTGGCGTCG GGAGGACAGA GTCCTGCAAC GGCCGATACG 
ACCACACATA CGGGAAGTAC AGACGATGGG CTGTCGCCAG CGCCGTCGAA TTGTTCGGTG 
ACGTGCGATA GTGCAGGGAG ACAGCCGGCT TGCACGATGG CGATGGTGCA CTGCATATCC 
AGCAGCGAGC CCGATTCGGC TGATCTGAAC ACCCAAGGCT CTGACCATGA CTGGTCCCGA 
GCGTCGTCTA TCCCTCAGCA AAAAAGCGAC TTGTCGGAGA TACATTTCCC CAAAAAACAA 
AACACTCCGA CCCACGTGGC TTACGCCGAA TCGTCGACAA TGATGGCCGG TTTGCATTTT 
ATCGGCGATC AAATGAAGGC CCAAGAGGCC GTCGTCAGTT CTCACAATTT CGATAAAATC 
GTACAGAAAC GCAAAAGCAG CGATACTTTG CTCGTCATGG AAACCCATCA CATGCATTCG 
TCGCTTTTCG ACGAGGAGGA AGATAATGAG AAACGTGAAA AGTCAGAACA TGACTTTGTT 
GTTGGCGATT TGGTTTGGGG GCCGGCTAAA GCAAGTCCAG CTTGGCCTGG CAAAATAATA 
CAAGTCGACG GGAACGATGT TTCGGTTAAA TGGTTCGGCA GCGAAAAATT AATTACAAAA 
TTAGACAGAG GTTCCTTGCA GACACTCACT GAAGGTCTCG ATGCTCACCA CCAAGCGCGC 
AAAAAATGCA GAACATCTCG CAAACTGAAT TCTCATCTGG AGAAGGCCAT CCAAGAGGCC 
ATGGCTGAGC TGGATAAGGA CTTGGAGCAA GAGACGAAGG AGGTGAAGCA GCAGAAGAAG 
AAAGCGCACC GAGTGAGAAG CGGTCATCGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC660029 ( XM_008202831.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008201053.1 Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X5, mRNA.

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CloneID	OTg01698
Clone ID Related Accession (Same CDS sequence)	XM_008202831.2
Accession Version	XM_008202831.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	methyl-CpG-binding domain protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450135.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008202831.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA AGCCTGAGCT GTCGCCCGCC CCCGGCGTCC AGAATGTGGT AGTGTACGTT 
TCGAACGACA ACTTCGCCCA AGCGTACGCC GTCGGGGGGC AGAATGCGCC CCAGTACGGC 
CCCCCGAAGC CCTACCCCGA GAAGCGGCCC GACTACCCCG AGGGCTACTT CGTCTCGTCC 
AACGGCTACG GATACGAGGA GCGCCCGGCC AAGGAGTGCG ACTACGACCC GGCCTATGTG 
ACGGTCATGG GGGCCGAGGG GCCCCCGCAG AGTGTGCGGT GTGATTCAGT GCGGAGCGAG 
ACGGCCGAGT CGAGCTGCAG CAGCTTGAGC TCGGCCGACG AGGGGATGGT GCTAGTACAG 
AACAATCAAC CAGAGATGGT GGTGTATGAT CCCAGTGTTA GTGTTAGACC CGGTGTCGTG 
TTTGTGGGGG CCGGCGTCCA CCACCACCCC AATGTCGCCG CCACCGCCGC CGCCAACCTA 
CCCGCCGCCC AACAGCCCTC CATCACTGTG CCCTACGGCT GGAAGAGGAT TCTCAACAAC 
GGATCCATCA TGTACATAAG CCCAAGTAAC ACAGCTTTAA GTTCTCCGGA ACAAGTCAAA 
GAATATCTCC TCACGTCGGG CACTTGCAAG TGTGGATTAG AATGTCATTT CAAATATGAC 
AACACTTTTA ATTTCGATCC TAAGGTGGCC AGTAAACCGT GGACGCTATC ACCGGACACC 
AATACAGGGG ACCTGACCAA ATTGTGCAAC CACAAGAGAA AAATCATCGC CATGGCCTCG 
TTGGAGTGCA AGCCCCCGGA CCCCGCCGCC AAAATGCGAA AAGACTGCAA CAATAAGAAA 
ACCAAGCGAA AAGTCGGCAT GCCTTACGCC GGTTGTGTTT CTTTATCGCA AATTTTCACT 
CGTCCGGACA AACTCGCTTT GAGTCAACAG CATTTCAACA AGGAAGGTCT CGGAAATCAA 
GCTCAGGTCG TGTGGCCTCA GTCACCGACT AGCAGCTCTC AAATGGGTAA TCGTCTGCAG 
AATTACCCCG AGAGCCAGCA GATGGTCCGA TATCAAAACC AGGAGCAACA GAATCGAATC 
GTTAACGGTT CGCCCATGGT GGTCGGTGAT AACAGTACCA TGATGGTACC CCAACAACAG 
TCCAACATAT CGCAACAGAT CCACCAGAAC CACAATGGTC AAAATCCCAG CGTTCCTCAG 
CAGCACATTA TCGGGCCCAA CGGCCAAGTG ATAAGTCTCC AAGGGGCCCT TAACTCGAAT 
CAGGTCAATA ATGGAGTGGT CATACAGGGT CAAAACGTCG TTCCTAATCC AAATCAATCC 
CGAGTTTTCA TCAACAGCCA ACCAAGCGAA CCCAGTGGAC CAGGCCGGCC CAACATCCAG 
ATGATTCCCG TAGGAGTCAC GAATCAAAGA GATGCCCAAA ACCGCCTCCA CCACTTCTAC 
AACAACCAAC AATACCCACA GATACAATAC GACGCGTCGA AGCAAATGCG ACCGACATTT 
ATGCCCGGCC ACAACCAAAT GAACGGTTTT CGCCCGCAAA ATAATCAGAT TATGCAAGCG 
CCGGTTATGA TTAACAACCA TCAAAAGATG CCATGGCCTA ATCGCATTCA AAATTCGCCC 
AATGCCTACG AAAGAGTGCC GCCGCTGCAT CCACACACAC CATCGCCCAC TATGTGGCAA 
GACGAGGTCA AACGGAAAAA GGTCAAATTG GGGAAAATCG TTAAAAATCG GCCGTATCAT 
ATGATGGATT CGGCGTTGCA TGCACCCTGT CCCAACATTG ACGTGCGGCA AATACCAGCC 
GAAAACGGCC GACAGGTTAT TATCAACCAG AATTCGGCCA GTCCCTCGTT TATGGAGGAC 
CCCAGTGGGT ATTTAGCCCA GCAGACGGCC CTGCTCAATA ATACCATCAA CAGACAAACA 
GGGGTGAACG CGTGCGCGGG CATGGTGTGC AGCAGCCCGC AGCAGCCCCA CCTAACACCA 
CCGAATTCGC AAACAAACGA GGTGCCTTTA CCCAGCAACG TGGTGATATC TCATATGAAA 
CAAACGCAAA ACATTACAAA TCGAACCAAT CAAACGTTAC ACGTAGTAAA AAGTCAAGTT 
CCTCAAGTAA TGTCCCAGCA GTATAATCAA ATGATGGCGG CTCACGCCGA CAGTAGCGTC 
ACCAACGACC AATGTCAAAA TTGCGACAGT CATAAAGGCC ATTCGAGACC TAACAGTCAA 
CCTGGAACTC CGAGTTCGTC GGCTATTGAC GATCCGGTGA CATCATCGAC TTATTCCGAC 
AAACAAAATA ATCAATCACC GGATTCAAGA CCGATTCAGG GCGGTACTGT TAGTACTAGC 
AATGTATCGC CATTGGATGG ATCACAATCA GATCCACCTA CACCTAACCC ACACACTCCA 
ACGCCTCCTA GGAATGATAA TATGCCATAT GCTGTCTACC AATCGCGTGA GGTTTGCAGT 
ACGAATTACC CACCGAAAAG TGACTGTTGT TCGAAAATGA ACACGCCGTA TATTTCGAGT 
ATAGTTACGA CGATGGCAAG TGGACGAACA GTTGGTAGTA ATACCATAAC GTCGGTTTTG 
GCCGGTCGTG CCAACACTGC CACAGTTTCA GTCAACAGTC CTGCTAACCT CCCAGCATCC 
ACAACCCCAA CAACAGTCAC ACAAACCGTT TCAAAATCCC CACTTGAAAT GGTCCAAAGC 
GTCGTCAGTA GCATACAAGT CCCTCAGTCT CAAACCAATC CCCAAATCTC TCCACCACAT 
ATCGTCAAAC ATTCCCCCAC CGGGTTACCT CCTGGCCATA TCCTTGTCTC AAGCGGCGGC 
CAGTTAATAA TGGCAAACAC CGGAAACAGC CAATCGGGCG TTATGCCACC CCCACCCCCC 
AAACTAGTCT CCAATCAAAC CGCAATGCCG CCAATGTCCG TCTCACCAAT GATCACAAAT 
GTCACTGCAG CGGTCAGTCA GGTCATACCG GCAGTAGCTC AGCAAGTTTT AGGCCAACAA 
ACCGTTTTAG TGAACGCCTT ACCCACACCT TTTGTCTTAC AAGGCGGAGT CACAATGACC 
ATGGACGGGT CCATGGTCGG CCAAAACATG CAATTACCTC AAATCGTCGC CGGAAATGTG 
ATACAACAAC AAATCCAACT GGATAACAGT GACCCACGAC GTACCACCGC GCTACTCTCA 
CCCGAAACCA AGAAAAAAGG AAAGAAACGA AAAATGTCAC AAACAGTGGC CAACATGCTT 
CACATAGCTG CCCAGCAAAA CTCCGGCGTT GTGATGTCAC AACAAGGCTT CCCGCAGCAA 
ATTCAAATGG CTCATAGTCC GCAAGGTCTA GCCACGGGCC CAGTGATGCA AGCCTTGACC 
ATAGTCCCCA GCAAAACAGG GGGACCCCCA CAGATCGTAA TGAACGGGCA ACCCATGACC 
AACACCAGTC CCCAACTCAT TGCAAACTCG CAACCGGCGC AACAAATCAA CCTCCTTCAG 
CCGGTGAATC TCATCAATGG CACCACCGGA GTGGTGCAAA ATTTCCCCAC GATTCAACAA 
TTCATAGTCC CGAATTTAGG CGGCATGGTT ATGAACCCGG ACGGCACCGC CACTATATTA 
CAGGACACTT CAAATATCGG CATGCAGTTA CAACTACAGA ACGTTAACGG ACAAAACGTC 
CTGACTCCGG TCCAAAACAA CGGAATGTTC AACGGGGGTC AGAGTATTCT AGCAGCGAGT 
CCGGCCGGCA TGGTCATTAG GGCCCCCACC TCGCAGGGGA AATTAATCCA GCAGCAGCAG 
CACAGTCCAG GTGCCCAGTT TTTGTCACCC AACGGGGGCC AGTTTGTCGT CAATGGGACA 
CAATTCAGCG GCCAGTTGAG CCCGCTTGTG GCTAGTGTGA GTCCGAGTCA GCAAGTTACC 
TTTGGGACGT CCACGCCGCA GATTAGACCA ACTCAGGGTC AACAAGAATT TATACAGTGT 
GGTCAAATGG GCCAGACTTT GATGGTTCCG TGTGCACCAA CGGTCGCCGT TTCCTCCTCG 
CAAAACACGA CTTTTGTCCA GCAAAACACT ACGATTGTTC AGCAGCAAAC CACGATGGTT 
TCGAATAGTC AACAGTTACA GAATTTCCAG AATAATGGAG GGAATCGAAC GACTGTGAAT 
GTGGATCAGA ATTTTATCAT CGCGAATGAT AATAAGCAAG TGCAGGCGCT TGTTCAGGTT 
CAGAGGGAGA GTCCCGGGAC GAATTATAGA CAGTCGGTGT CGACGCAAAC AGCGGTTAAT 
CAGAATGCAC AGACAGTGAC CACGTTTTGT CAGACGTCCA CGCTGTGTGC GGGGAGTCCC 
CCGGATACTA CAACGCATAG TCCGTTGGCG TCGGGAGGAC AGAGTCCTGC AACGGCCGAT 
ACGACCACAC ATACGGGAAG TACAGACGAT GGGCTGTCGC CAGCGCCGTC GAATTGTTCG 
GTGACGTGCG ATAGTGCAGG GAGACAGCCG GCTTGCACGA TGGCGATGGT GCACTGCATA 
TCCAGCAGCG AGCCCGATTC GGCTGATCTG AACACCCAAG GCTCTGACCA TGACTGGTCC 
CGAGCGTCGT CTATCCCTCA GCAAAAAAGC GACTTGTCGG AGATACATTT CCCCAAAAAA 
CAAAACACTC CGACCCACGT GGCTTACGCC GAATCGTCGA CAATGATGGC CGGTTTGCAT 
TTTATCGGCG ATCAAATGAA GGCCCAAGAG GCCGTCGTCA GTTCTCACAA TTTCGATAAA 
ATCGTACAGA AACGCAAAAG CAGCGATACT TTGCTCGTCA TGGAAACCCA TCACATGCAT 
TCGTCGCTTT TCGACGAGGA GGAAGATCTC GCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008201053.1
CDS	388..5226
Translation	MAGKPELSPAPGVQNVVVYVSNDNFAQAYAVGGQNAPQYGPPKPYPEKRPDYPEGYFVSSNGYGYEERPAKECDYDPAYVTVMGAEGPPQSVRCDSVRSETAESSCSSLSSADEGMVLVQNNQPEMVVYDPSVSVRPGVVFVGAGVHHHPNVAATAAANLPAAQQPSITVPYGWKRILNNGSIMYISPSNTALSSPEQVKEYLLTSGTCKCGLECHFKYDNTFNFDPKVASKPWTLSPDTNTGDLTKLCNHKRKIIAMASLECKPPDPAAKMRKDCNNKKTKRKVGMPYAGCVSLSQIFTRPDKLALSQQHFNKEGLGNQAQVVWPQSPTSSSQMGNRLQNYPESQQMVRYQNQEQQNRIVNGSPMVVGDNSTMMVPQQQSNISQQIHQNHNGQNPSVPQQHIIGPNGQVISLQGALNSNQVNNGVVIQGQNVVPNPNQSRVFINSQPSEPSGPGRPNIQMIPVGVTNQRDAQNRLHHFYNNQQYPQIQYDASKQMRPTFMPGHNQMNGFRPQNNQIMQAPVMINNHQKMPWPNRIQNSPNAYERVPPLHPHTPSPTMWQDEVKRKKVKLGKIVKNRPYHMMDSALHAPCPNIDVRQIPAENGRQVIINQNSASPSFMEDPSGYLAQQTALLNNTINRQTGVNACAGMVCSSPQQPHLTPPNSQTNEVPLPSNVVISHMKQTQNITNRTNQTLHVVKSQVPQVMSQQYNQMMAAHADSSVTNDQCQNCDSHKGHSRPNSQPGTPSSSAIDDPVTSSTYSDKQNNQSPDSRPIQGGTVSTSNVSPLDGSQSDPPTPNPHTPTPPRNDNMPYAVYQSREVCSTNYPPKSDCCSKMNTPYISSIVTTMASGRTVGSNTITSVLAGRANTATVSVNSPANLPASTTPTTVTQTVSKSPLEMVQSVVSSIQVPQSQTNPQISPPHIVKHSPTGLPPGHILVSSGGQLIMANTGNSQSGVMPPPPPKLVSNQTAMPPMSVSPMITNVTAAVSQVIPAVAQQVLGQQTVLVNALPTPFVLQGGVTMTMDGSMVGQNMQLPQIVAGNVIQQQIQLDNSDPRRTTALLSPETKKKGKKRKMSQTVANMLHIAAQQNSGVVMSQQGFPQQIQMAHSPQGLATGPVMQALTIVPSKTGGPPQIVMNGQPMTNTSPQLIANSQPAQQINLLQPVNLINGTTGVVQNFPTIQQFIVPNLGGMVMNPDGTATILQDTSNIGMQLQLQNVNGQNVLTPVQNNGMFNGGQSILAASPAGMVIRAPTSQGKLIQQQQHSPGAQFLSPNGGQFVVNGTQFSGQLSPLVASVSPSQQVTFGTSTPQIRPTQGQQEFIQCGQMGQTLMVPCAPTVAVSSSQNTTFVQQNTTIVQQQTTMVSNSQQLQNFQNNGGNRTTVNVDQNFIIANDNKQVQALVQVQRESPGTNYRQSVSTQTAVNQNAQTVTTFCQTSTLCAGSPPDTTTHSPLASGGQSPATADTTTHTGSTDDGLSPAPSNCSVTCDSAGRQPACTMAMVHCISSSEPDSADLNTQGSDHDWSRASSIPQQKSDLSEIHFPKKQNTPTHVAYAESSTMMAGLHFIGDQMKAQEAVVSSHNFDKIVQKRKSSDTLLVMETHHMHSSLFDEEEDLAN

Target ORF information:

RefSeq Version	XM_008202831.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008202831.2

ORF Insert Sequence:

ATGGCAGGAA AGCCTGAGCT GTCGCCCGCC CCCGGCGTCC AGAATGTGGT AGTGTACGTT 
TCGAACGACA ACTTCGCCCA AGCGTACGCC GTCGGGGGGC AGAATGCGCC CCAGTACGGC 
CCCCCGAAGC CCTACCCCGA GAAGCGGCCC GACTACCCCG AGGGCTACTT CGTCTCGTCC 
AACGGCTACG GATACGAGGA GCGCCCGGCC AAGGAGTGCG ACTACGACCC GGCCTATGTG 
ACGGTCATGG GGGCCGAGGG GCCCCCGCAG AGTGTGCGGT GTGATTCAGT GCGGAGCGAG 
ACGGCCGAGT CGAGCTGCAG CAGCTTGAGC TCGGCCGACG AGGGGATGGT GCTAGTACAG 
AACAATCAAC CAGAGATGGT GGTGTATGAT CCCAGTGTTA GTGTTAGACC CGGTGTCGTG 
TTTGTGGGGG CCGGCGTCCA CCACCACCCC AATGTCGCCG CCACCGCCGC CGCCAACCTA 
CCCGCCGCCC AACAGCCCTC CATCACTGTG CCCTACGGCT GGAAGAGGAT TCTCAACAAC 
GGATCCATCA TGTACATAAG CCCAAGTAAC ACAGCTTTAA GTTCTCCGGA ACAAGTCAAA 
GAATATCTCC TCACGTCGGG CACTTGCAAG TGTGGATTAG AATGTCATTT CAAATATGAC 
AACACTTTTA ATTTCGATCC TAAGGTGGCC AGTAAACCGT GGACGCTATC ACCGGACACC 
AATACAGGGG ACCTGACCAA ATTGTGCAAC CACAAGAGAA AAATCATCGC CATGGCCTCG 
TTGGAGTGCA AGCCCCCGGA CCCCGCCGCC AAAATGCGAA AAGACTGCAA CAATAAGAAA 
ACCAAGCGAA AAGTCGGCAT GCCTTACGCC GGTTGTGTTT CTTTATCGCA AATTTTCACT 
CGTCCGGACA AACTCGCTTT GAGTCAACAG CATTTCAACA AGGAAGGTCT CGGAAATCAA 
GCTCAGGTCG TGTGGCCTCA GTCACCGACT AGCAGCTCTC AAATGGGTAA TCGTCTGCAG 
AATTACCCCG AGAGCCAGCA GATGGTCCGA TATCAAAACC AGGAGCAACA GAATCGAATC 
GTTAACGGTT CGCCCATGGT GGTCGGTGAT AACAGTACCA TGATGGTACC CCAACAACAG 
TCCAACATAT CGCAACAGAT CCACCAGAAC CACAATGGTC AAAATCCCAG CGTTCCTCAG 
CAGCACATTA TCGGGCCCAA CGGCCAAGTG ATAAGTCTCC AAGGGGCCCT TAACTCGAAT 
CAGGTCAATA ATGGAGTGGT CATACAGGGT CAAAACGTCG TTCCTAATCC AAATCAATCC 
CGAGTTTTCA TCAACAGCCA ACCAAGCGAA CCCAGTGGAC CAGGCCGGCC CAACATCCAG 
ATGATTCCCG TAGGAGTCAC GAATCAAAGA GATGCCCAAA ACCGCCTCCA CCACTTCTAC 
AACAACCAAC AATACCCACA GATACAATAC GACGCGTCGA AGCAAATGCG ACCGACATTT 
ATGCCCGGCC ACAACCAAAT GAACGGTTTT CGCCCGCAAA ATAATCAGAT TATGCAAGCG 
CCGGTTATGA TTAACAACCA TCAAAAGATG CCATGGCCTA ATCGCATTCA AAATTCGCCC 
AATGCCTACG AAAGAGTGCC GCCGCTGCAT CCACACACAC CATCGCCCAC TATGTGGCAA 
GACGAGGTCA AACGGAAAAA GGTCAAATTG GGGAAAATCG TTAAAAATCG GCCGTATCAT 
ATGATGGATT CGGCGTTGCA TGCACCCTGT CCCAACATTG ACGTGCGGCA AATACCAGCC 
GAAAACGGCC GACAGGTTAT TATCAACCAG AATTCGGCCA GTCCCTCGTT TATGGAGGAC 
CCCAGTGGGT ATTTAGCCCA GCAGACGGCC CTGCTCAATA ATACCATCAA CAGACAAACA 
GGGGTGAACG CGTGCGCGGG CATGGTGTGC AGCAGCCCGC AGCAGCCCCA CCTAACACCA 
CCGAATTCGC AAACAAACGA GGTGCCTTTA CCCAGCAACG TGGTGATATC TCATATGAAA 
CAAACGCAAA ACATTACAAA TCGAACCAAT CAAACGTTAC ACGTAGTAAA AAGTCAAGTT 
CCTCAAGTAA TGTCCCAGCA GTATAATCAA ATGATGGCGG CTCACGCCGA CAGTAGCGTC 
ACCAACGACC AATGTCAAAA TTGCGACAGT CATAAAGGCC ATTCGAGACC TAACAGTCAA 
CCTGGAACTC CGAGTTCGTC GGCTATTGAC GATCCGGTGA CATCATCGAC TTATTCCGAC 
AAACAAAATA ATCAATCACC GGATTCAAGA CCGATTCAGG GCGGTACTGT TAGTACTAGC 
AATGTATCGC CATTGGATGG ATCACAATCA GATCCACCTA CACCTAACCC ACACACTCCA 
ACGCCTCCTA GGAATGATAA TATGCCATAT GCTGTCTACC AATCGCGTGA GGTTTGCAGT 
ACGAATTACC CACCGAAAAG TGACTGTTGT TCGAAAATGA ACACGCCGTA TATTTCGAGT 
ATAGTTACGA CGATGGCAAG TGGACGAACA GTTGGTAGTA ATACCATAAC GTCGGTTTTG 
GCCGGTCGTG CCAACACTGC CACAGTTTCA GTCAACAGTC CTGCTAACCT CCCAGCATCC 
ACAACCCCAA CAACAGTCAC ACAAACCGTT TCAAAATCCC CACTTGAAAT GGTCCAAAGC 
GTCGTCAGTA GCATACAAGT CCCTCAGTCT CAAACCAATC CCCAAATCTC TCCACCACAT 
ATCGTCAAAC ATTCCCCCAC CGGGTTACCT CCTGGCCATA TCCTTGTCTC AAGCGGCGGC 
CAGTTAATAA TGGCAAACAC CGGAAACAGC CAATCGGGCG TTATGCCACC CCCACCCCCC 
AAACTAGTCT CCAATCAAAC CGCAATGCCG CCAATGTCCG TCTCACCAAT GATCACAAAT 
GTCACTGCAG CGGTCAGTCA GGTCATACCG GCAGTAGCTC AGCAAGTTTT AGGCCAACAA 
ACCGTTTTAG TGAACGCCTT ACCCACACCT TTTGTCTTAC AAGGCGGAGT CACAATGACC 
ATGGACGGGT CCATGGTCGG CCAAAACATG CAATTACCTC AAATCGTCGC CGGAAATGTG 
ATACAACAAC AAATCCAACT GGATAACAGT GACCCACGAC GTACCACCGC GCTACTCTCA 
CCCGAAACCA AGAAAAAAGG AAAGAAACGA AAAATGTCAC AAACAGTGGC CAACATGCTT 
CACATAGCTG CCCAGCAAAA CTCCGGCGTT GTGATGTCAC AACAAGGCTT CCCGCAGCAA 
ATTCAAATGG CTCATAGTCC GCAAGGTCTA GCCACGGGCC CAGTGATGCA AGCCTTGACC 
ATAGTCCCCA GCAAAACAGG GGGACCCCCA CAGATCGTAA TGAACGGGCA ACCCATGACC 
AACACCAGTC CCCAACTCAT TGCAAACTCG CAACCGGCGC AACAAATCAA CCTCCTTCAG 
CCGGTGAATC TCATCAATGG CACCACCGGA GTGGTGCAAA ATTTCCCCAC GATTCAACAA 
TTCATAGTCC CGAATTTAGG CGGCATGGTT ATGAACCCGG ACGGCACCGC CACTATATTA 
CAGGACACTT CAAATATCGG CATGCAGTTA CAACTACAGA ACGTTAACGG ACAAAACGTC 
CTGACTCCGG TCCAAAACAA CGGAATGTTC AACGGGGGTC AGAGTATTCT AGCAGCGAGT 
CCGGCCGGCA TGGTCATTAG GGCCCCCACC TCGCAGGGGA AATTAATCCA GCAGCAGCAG 
CACAGTCCAG GTGCCCAGTT TTTGTCACCC AACGGGGGCC AGTTTGTCGT CAATGGGACA 
CAATTCAGCG GCCAGTTGAG CCCGCTTGTG GCTAGTGTGA GTCCGAGTCA GCAAGTTACC 
TTTGGGACGT CCACGCCGCA GATTAGACCA ACTCAGGGTC AACAAGAATT TATACAGTGT 
GGTCAAATGG GCCAGACTTT GATGGTTCCG TGTGCACCAA CGGTCGCCGT TTCCTCCTCG 
CAAAACACGA CTTTTGTCCA GCAAAACACT ACGATTGTTC AGCAGCAAAC CACGATGGTT 
TCGAATAGTC AACAGTTACA GAATTTCCAG AATAATGGAG GGAATCGAAC GACTGTGAAT 
GTGGATCAGA ATTTTATCAT CGCGAATGAT AATAAGCAAG TGCAGGCGCT TGTTCAGGTT 
CAGAGGGAGA GTCCCGGGAC GAATTATAGA CAGTCGGTGT CGACGCAAAC AGCGGTTAAT 
CAGAATGCAC AGACAGTGAC CACGTTTTGT CAGACGTCCA CGCTGTGTGC GGGGAGTCCC 
CCGGATACTA CAACGCATAG TCCGTTGGCG TCGGGAGGAC AGAGTCCTGC AACGGCCGAT 
ACGACCACAC ATACGGGAAG TACAGACGAT GGGCTGTCGC CAGCGCCGTC GAATTGTTCG 
GTGACGTGCG ATAGTGCAGG GAGACAGCCG GCTTGCACGA TGGCGATGGT GCACTGCATA 
TCCAGCAGCG AGCCCGATTC GGCTGATCTG AACACCCAAG GCTCTGACCA TGACTGGTCC 
CGAGCGTCGT CTATCCCTCA GCAAAAAAGC GACTTGTCGG AGATACATTT CCCCAAAAAA 
CAAAACACTC CGACCCACGT GGCTTACGCC GAATCGTCGA CAATGATGGC CGGTTTGCAT 
TTTATCGGCG ATCAAATGAA GGCCCAAGAG GCCGTCGTCA GTTCTCACAA TTTCGATAAA 
ATCGTACAGA AACGCAAAAG CAGCGATACT TTGCTCGTCA TGGAAACCCA TCACATGCAT 
TCGTCGCTTT TCGACGAGGA GGAAGATCTC GCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC660029 ( XM_015985199.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015840685.1 Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X4, mRNA.

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CloneID	OTg17004
Clone ID Related Accession (Same CDS sequence)	XM_015985199.1
Accession Version	XM_015985199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	methyl-CpG-binding domain protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450135.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAA AGCCTGAGCT GTCGCCCGCC CCCGGCGTCC AGAATGTGGT AGTGTACGTT 
TCGAACGACA ACTTCGCCCA AGCGTACGCC GTCGGGGGGC AGAATGCGCC CCAGTACGGC 
CCCCCGAAGC CCTACCCCGA GAAGCGGCCC GACTACCCCG AGGGCTACTT CGTCTCGTCC 
AACGGCTACG GATACGAGGA GCGCCCGGCC AAGGAGTGCG ACTACGACCC GGCCTATGTG 
ACGGTCATGG GGGCCGAGGG GCCCCCGCAG AGTGTGCGGT GTGATTCAGT GCGGAGCGAG 
ACGGCCGAGT CGAGCTGCAG CAGCTTGAGC TCGGCCGACG AGGGGATGGT GCTAGTACAG 
AACAATCAAC CAGAGATGGT GGTGTATGAT CCCAGTGTTA GTGTTAGACC CGGTGTCGTG 
TTTGTGGGGG CCGGCGTCCA CCACCACCCC AATGTCGCCG CCACCGCCGC CGCCAACCTA 
CCCGCCGCCC AACAGCCCTC CATCACTGTG CCCTACGGCT GGAAGAGGAT TCTCAACAAC 
GGATCCATCA TGTACATAAG CCCAAGTAAC ACAGCTTTAA GTTCTCCGGA ACAAGTCAAA 
GAATATCTCC TCACGTCGGG CACTTGCAAG TGTGGATTAG AATGTCATTT CAAATATGAC 
AACACTTTTA ATTTCGATCC TAAGGTGGCC AGTAAACCGT GGACGCTATC ACCGGACACC 
AATACAGGGG ACCTGACCAA ATTGTGCAAC CACAAGAGAA AAATCATCGC CATGGCCTCG 
TTGGAGTGCA AGCCCCCGGA CCCCGCCGCC AAAATGCGAA AAGACTGCAA CAATAAGAAA 
ACCAAGCGAA AAGTCGGCAT GCCTTACGCC GGTTGTGTTT CTTTATCGCA AATTTTCACT 
CGTCCGGACA AACTCGCTTT GAGTCAACAG CATTTCAACA AGGAAGGTCT CGGAAATCAA 
GCTCAGGTCG TGTGGCCTCA GTCACCGACT AGCAGCTCTC AAATGGGTAA TCGTCTGCAG 
AATTACCCCG AGAGCCAGCA GATGGTCCGA TATCAAAACC AGGAGCAACA GAATCGAATC 
GTTAACGGTT CGCCCATGGT GGTCGGTGAT AACAGTACCA TGATGGTACC CCAACAACAG 
TCCAACATAT CGCAACAGAT CCACCAGAAC CACAATGGTC AAAATCCCAG CGTTCCTCAG 
CAGCACATTA TCGGGCCCAA CGGCCAAGTG ATAAGTCTCC AAGGGGCCCT TAACTCGAAT 
CAGGTCAATA ATGGAGTGGT CATACAGGGT CAAAACGTCG TTCCTAATCC AAATCAATCC 
CGAGTTTTCA TCAACAGCCA ACCAAGCGAA CCCAGTGGAC CAGGCCGGCC CAACATCCAG 
ATGATTCCCG TAGGAGTCAC GAATCAAAGA GATGCCCAAA ACCGCCTCCA CCACTTCTAC 
AACAACCAAC AATACCCACA GATACAATAC GACGCGTCGA AGCAAATGCG ACCGACATTT 
ATGCCCGGCC ACAACCAAAT GAACGGTTTT CGCCCGCAAA ATAATCAGAT TATGCAAGCG 
CCGGTTATGA TTAACAACCA TCAAAAGATG CCATGGCCTA ATCGCATTCA AAATTCGCCC 
AATGCCTACG AAAGAGTGCC GCCGCTGCAT CCACACACAC CATCGCCCAC TATGTGGCAA 
GACGAGGTCA AACGGAAAAA GGTCAAATTG GGGAAAATCG TTAAAAATCG GCCGTATCAT 
ATGATGGATT CGGCGTTGCA TGCACCCTGT CCCAACATTG ACGTGCGGCA AATACCAGCC 
GAAAACGGCC GACAGGTTAT TATCAACCAG AATTCGGCCA GTCCCTCGTT TATGGAGGAC 
CCCAGTGGGT ATTTAGCCCA GCAGACGGCC CTGCTCAATA ATACCATCAA CAGACAAACA 
GGGGTGAACG CGTGCGCGGG CATGGTGTGC AGCAGCCCGC AGCAGCCCCA CCTAACACCA 
CCGAATTCGC AAACAAACGA GGTGCCTTTA CCCAGCAACG TGGTGATATC TCATATGAAA 
CAAACGCAAA ACATTACAAA TCGAACCAAT CAAACGTTAC ACGTAGTAAA AAGTCAAGTT 
CCTCAAGTAA TGTCCCAGCA GTATAATCAA ATGATGGCGG CTCACGCCGA CAGTAGCGTC 
ACCAACGACC AATGTCAAAA TTGCGACAGT CATAAAGGCC ATTCGAGACC TAACAGTCAA 
CCTGGAACTC CGAGTTCGTC GGCTATTGAC GATCCGGTGA CATCATCGAC TTATTCCGAC 
AAACAAAATA ATCAATCACC GGATTCAAGA CCGATTCAGG GCGGTACTGT TAGTACTAGC 
AATGTATCGC CATTGGATGG ATCACAATCA GATCCACCTA CACCTAACCC ACACACTCCA 
ACGCCTCCTA GGAATGATAA TATGCCATAT GCTGTCTACC AATCGCGTGA GGTTTGCAGT 
ACGAATTACC CACCGAAAAG TGACTGTTGT TCGAAAATGA ACACGCCGTA TATTTCGAGT 
ATAGTTACGA CGATGGCAAG TGGACGAACA GTTGGTAGTA ATACCATAAC GTCGGTTTTG 
GCCGGTCGTG CCAACACTGC CACAGTTTCA GTCAACAGTC CTGCTAACCT CCCAGCATCC 
ACAACCCCAA CAACAGTCAC ACAAACCGTT TCAAAATCCC CACTTGAAAT GGTCCAAAGC 
GTCGTCAGTA GCATACAAGT CCCTCAGTCT CAAACCAATC CCCAAATCTC TCCACCACAT 
ATCGTCAAAC ATTCCCCCAC CGGGTTACCT CCTGGCCATA TCCTTGTCTC AAGCGGCGGC 
CAGTTAATAA TGGCAAACAC CGGAAACAGC CAATCGGGCG TTATGCCACC CCCACCCCCC 
AAACTAGTCT CCAATCAAAC CGCAATGCCG CCAATGTCCG TCTCACCAAT GATCACAAAT 
GTCACTGCAG CGGTCAGTCA GGTCATACCG GCAGTAGCTC AGCAAGTTTT AGGCCAACAA 
ACCGTTTTAG TGAACGCCTT ACCCACACCT TTTGTCTTAC AAGGCGGAGT CACAATGACC 
ATGGACGGGT CCATGGTCGG CCAAAACATG CAATTACCTC AAATCGTCGC CGGAAATGTG 
ATACAACAAC AAATCCAACT GGATAACAGT GACCCACGAC GTACCACCGC GCTACTCTCA 
CCCGAAACCA AGAAAAAAGG AAAGAAACGA AAAATGTCAC AAACAGTGGC CAACATGCTT 
CACATAGCTG CCCAGCAAAA CTCCGGCGTT GTGATGTCAC AACAAGGCTT CCCGCAGCAA 
ATTCAAATGG CTCATAGTCC GCAAGGTCTA GCCACGGGCC CAGTGATGCA AGCCTTGACC 
ATAGTCCCCA GCAAAACAGG GGGACCCCCA CAGATCGTAA TGAACGGGCA ACCCATGACC 
AACACCAGTC CCCAACTCAT TGCAAACTCG CAACCGGCGC AACAAATCAA CCTCCTTCAG 
CCGGTGAATC TCATCAATGG CACCACCGGA GTGGTGCAAA ATTTCCCCAC GATTCAACAA 
TTCATAGTCC CGAATTTAGG CGGCATGGTT ATGAACCCGG ACGGCACCGC CACTATATTA 
CAGGACACTT CAAATATCGG CATGCAGTTA CAACTACAGA ACGTTAACGG ACAAAACGTC 
CTGACTCCGG TCCAAAACAA CGGAATGTTC AACGGGGGTC AGAGTATTCT AGCAGCGAGT 
CCGGCCGGCA TGGTCATTAG GGCCCCCACC TCGCAGGGGA AATTAATCCA GCAGCAGCAG 
CACAGTCCAG GTGCCCAGTT TTTGTCACCC AACGGGGGCC AGTTTGTCGT CAATGGGACA 
CAATTCAGCG GCCAGTTGAG CCCGCTTGTG GCTAGTGTGA GTCCGAGTCA GCAAGTTACC 
TTTGGGACGT CCACGCCGCA GATTAGACCA ACTCAGGGTC AACAAGAATT TATACAGTGT 
GGTCAAATGG GCCAGACTTT GATGGTTCCG TGTGCACCAA CGGTCGCCGT TTCCTCCTCG 
CAAAACACGA CTTTTGTCCA GCAAAACACT ACGATTGTTC AGCAGCAAAC CACGATGGTT 
TCGAATAGTC AACAGTTACA GAATTTCCAG AATAATGGAG GGAATCGAAC GACTGTGAAT 
GTGGATCAGA ATTTTATCAT CGCGAATGAT AATAAGCAAG TGCAGGCGCT TGTTCAGGTT 
CAGAGGGAGA GTCCCGGGAC GAATTATAGA CAGTCGGTGT CGACGCAAAC AGCGGTTAAT 
CAGAATGCAC AGACAGTGAC CACGTTTTGT CAGACGTCCA CGCTGTGTGC GGGGAGTCCC 
CCGGATACTA CAACGCATAG TCCGTTGGCG TCGGGAGGAC AGAGTCCTGC AACGGCCGAT 
ACGACCACAC ATACGGGAAG TACAGACGAT GGGCTGTCGC CAGCGCCGTC GAATTGTTCG 
GTGACGTGCG ATAGTGCAGG GAGACAGCCG GCTTGCACGA TGGCGATGGT GCACTGCATA 
TCCAGCAGCG AGCCCGATTC GGCTGATCTG AACACCCAAG GCTCTGACCA TGACTGGTCC 
CGAGCGTCGT CTATCCCTCA GCAAAAAAGC GACTTGTCGG AGATACATTT CCCCAAAAAA 
CAAAACACTC CGACCCACGT GGCTTACGCC GAATCGTCGA CAATGATGGC CGGTTTGCAT 
TTTATCGGCG ATCAAATGAA GGCCCAAGAG GCCGTCGTCA GTTCTCACAA TTTCGATAAA 
ATCGTACAGA AACGCAAAAG CAGCGATACT TTGCTCGTCA TGGAAACCCA TCACATGCAT 
TCGTCGCTTT TCGACGAGGA GGAAGATAAT GAGAAACGTG AAAAGTCAGA ACATGACTTT 
GTTGTTGGCG ATTTGGTTTG GGGGCCGGCT AAAGCAAGTC CAGCTTGGCC TGGCAAAATA 
ATACAAGTCG ACGGGAACGA TGTTTCGGTT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015840685.1
CDS	388..5343
Translation	MAGKPELSPAPGVQNVVVYVSNDNFAQAYAVGGQNAPQYGPPKPYPEKRPDYPEGYFVSSNGYGYEERPAKECDYDPAYVTVMGAEGPPQSVRCDSVRSETAESSCSSLSSADEGMVLVQNNQPEMVVYDPSVSVRPGVVFVGAGVHHHPNVAATAAANLPAAQQPSITVPYGWKRILNNGSIMYISPSNTALSSPEQVKEYLLTSGTCKCGLECHFKYDNTFNFDPKVASKPWTLSPDTNTGDLTKLCNHKRKIIAMASLECKPPDPAAKMRKDCNNKKTKRKVGMPYAGCVSLSQIFTRPDKLALSQQHFNKEGLGNQAQVVWPQSPTSSSQMGNRLQNYPESQQMVRYQNQEQQNRIVNGSPMVVGDNSTMMVPQQQSNISQQIHQNHNGQNPSVPQQHIIGPNGQVISLQGALNSNQVNNGVVIQGQNVVPNPNQSRVFINSQPSEPSGPGRPNIQMIPVGVTNQRDAQNRLHHFYNNQQYPQIQYDASKQMRPTFMPGHNQMNGFRPQNNQIMQAPVMINNHQKMPWPNRIQNSPNAYERVPPLHPHTPSPTMWQDEVKRKKVKLGKIVKNRPYHMMDSALHAPCPNIDVRQIPAENGRQVIINQNSASPSFMEDPSGYLAQQTALLNNTINRQTGVNACAGMVCSSPQQPHLTPPNSQTNEVPLPSNVVISHMKQTQNITNRTNQTLHVVKSQVPQVMSQQYNQMMAAHADSSVTNDQCQNCDSHKGHSRPNSQPGTPSSSAIDDPVTSSTYSDKQNNQSPDSRPIQGGTVSTSNVSPLDGSQSDPPTPNPHTPTPPRNDNMPYAVYQSREVCSTNYPPKSDCCSKMNTPYISSIVTTMASGRTVGSNTITSVLAGRANTATVSVNSPANLPASTTPTTVTQTVSKSPLEMVQSVVSSIQVPQSQTNPQISPPHIVKHSPTGLPPGHILVSSGGQLIMANTGNSQSGVMPPPPPKLVSNQTAMPPMSVSPMITNVTAAVSQVIPAVAQQVLGQQTVLVNALPTPFVLQGGVTMTMDGSMVGQNMQLPQIVAGNVIQQQIQLDNSDPRRTTALLSPETKKKGKKRKMSQTVANMLHIAAQQNSGVVMSQQGFPQQIQMAHSPQGLATGPVMQALTIVPSKTGGPPQIVMNGQPMTNTSPQLIANSQPAQQINLLQPVNLINGTTGVVQNFPTIQQFIVPNLGGMVMNPDGTATILQDTSNIGMQLQLQNVNGQNVLTPVQNNGMFNGGQSILAASPAGMVIRAPTSQGKLIQQQQHSPGAQFLSPNGGQFVVNGTQFSGQLSPLVASVSPSQQVTFGTSTPQIRPTQGQQEFIQCGQMGQTLMVPCAPTVAVSSSQNTTFVQQNTTIVQQQTTMVSNSQQLQNFQNNGGNRTTVNVDQNFIIANDNKQVQALVQVQRESPGTNYRQSVSTQTAVNQNAQTVTTFCQTSTLCAGSPPDTTTHSPLASGGQSPATADTTTHTGSTDDGLSPAPSNCSVTCDSAGRQPACTMAMVHCISSSEPDSADLNTQGSDHDWSRASSIPQQKSDLSEIHFPKKQNTPTHVAYAESSTMMAGLHFIGDQMKAQEAVVSSHNFDKIVQKRKSSDTLLVMETHHMHSSLFDEEEDNEKREKSEHDFVVGDLVWGPAKASPAWPGKIIQVDGNDVSVK

Target ORF information:

RefSeq Version	XM_015985199.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum methyl-CpG-binding domain protein 5 (LOC660029), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015985199.1

ORF Insert Sequence:

ATGGCAGGAA AGCCTGAGCT GTCGCCCGCC CCCGGCGTCC AGAATGTGGT AGTGTACGTT 
TCGAACGACA ACTTCGCCCA AGCGTACGCC GTCGGGGGGC AGAATGCGCC CCAGTACGGC 
CCCCCGAAGC CCTACCCCGA GAAGCGGCCC GACTACCCCG AGGGCTACTT CGTCTCGTCC 
AACGGCTACG GATACGAGGA GCGCCCGGCC AAGGAGTGCG ACTACGACCC GGCCTATGTG 
ACGGTCATGG GGGCCGAGGG GCCCCCGCAG AGTGTGCGGT GTGATTCAGT GCGGAGCGAG 
ACGGCCGAGT CGAGCTGCAG CAGCTTGAGC TCGGCCGACG AGGGGATGGT GCTAGTACAG 
AACAATCAAC CAGAGATGGT GGTGTATGAT CCCAGTGTTA GTGTTAGACC CGGTGTCGTG 
TTTGTGGGGG CCGGCGTCCA CCACCACCCC AATGTCGCCG CCACCGCCGC CGCCAACCTA 
CCCGCCGCCC AACAGCCCTC CATCACTGTG CCCTACGGCT GGAAGAGGAT TCTCAACAAC 
GGATCCATCA TGTACATAAG CCCAAGTAAC ACAGCTTTAA GTTCTCCGGA ACAAGTCAAA 
GAATATCTCC TCACGTCGGG CACTTGCAAG TGTGGATTAG AATGTCATTT CAAATATGAC 
AACACTTTTA ATTTCGATCC TAAGGTGGCC AGTAAACCGT GGACGCTATC ACCGGACACC 
AATACAGGGG ACCTGACCAA ATTGTGCAAC CACAAGAGAA AAATCATCGC CATGGCCTCG 
TTGGAGTGCA AGCCCCCGGA CCCCGCCGCC AAAATGCGAA AAGACTGCAA CAATAAGAAA 
ACCAAGCGAA AAGTCGGCAT GCCTTACGCC GGTTGTGTTT CTTTATCGCA AATTTTCACT 
CGTCCGGACA AACTCGCTTT GAGTCAACAG CATTTCAACA AGGAAGGTCT CGGAAATCAA 
GCTCAGGTCG TGTGGCCTCA GTCACCGACT AGCAGCTCTC AAATGGGTAA TCGTCTGCAG 
AATTACCCCG AGAGCCAGCA GATGGTCCGA TATCAAAACC AGGAGCAACA GAATCGAATC 
GTTAACGGTT CGCCCATGGT GGTCGGTGAT AACAGTACCA TGATGGTACC CCAACAACAG 
TCCAACATAT CGCAACAGAT CCACCAGAAC CACAATGGTC AAAATCCCAG CGTTCCTCAG 
CAGCACATTA TCGGGCCCAA CGGCCAAGTG ATAAGTCTCC AAGGGGCCCT TAACTCGAAT 
CAGGTCAATA ATGGAGTGGT CATACAGGGT CAAAACGTCG TTCCTAATCC AAATCAATCC 
CGAGTTTTCA TCAACAGCCA ACCAAGCGAA CCCAGTGGAC CAGGCCGGCC CAACATCCAG 
ATGATTCCCG TAGGAGTCAC GAATCAAAGA GATGCCCAAA ACCGCCTCCA CCACTTCTAC 
AACAACCAAC AATACCCACA GATACAATAC GACGCGTCGA AGCAAATGCG ACCGACATTT 
ATGCCCGGCC ACAACCAAAT GAACGGTTTT CGCCCGCAAA ATAATCAGAT TATGCAAGCG 
CCGGTTATGA TTAACAACCA TCAAAAGATG CCATGGCCTA ATCGCATTCA AAATTCGCCC 
AATGCCTACG AAAGAGTGCC GCCGCTGCAT CCACACACAC CATCGCCCAC TATGTGGCAA 
GACGAGGTCA AACGGAAAAA GGTCAAATTG GGGAAAATCG TTAAAAATCG GCCGTATCAT 
ATGATGGATT CGGCGTTGCA TGCACCCTGT CCCAACATTG ACGTGCGGCA AATACCAGCC 
GAAAACGGCC GACAGGTTAT TATCAACCAG AATTCGGCCA GTCCCTCGTT TATGGAGGAC 
CCCAGTGGGT ATTTAGCCCA GCAGACGGCC CTGCTCAATA ATACCATCAA CAGACAAACA 
GGGGTGAACG CGTGCGCGGG CATGGTGTGC AGCAGCCCGC AGCAGCCCCA CCTAACACCA 
CCGAATTCGC AAACAAACGA GGTGCCTTTA CCCAGCAACG TGGTGATATC TCATATGAAA 
CAAACGCAAA ACATTACAAA TCGAACCAAT CAAACGTTAC ACGTAGTAAA AAGTCAAGTT 
CCTCAAGTAA TGTCCCAGCA GTATAATCAA ATGATGGCGG CTCACGCCGA CAGTAGCGTC 
ACCAACGACC AATGTCAAAA TTGCGACAGT CATAAAGGCC ATTCGAGACC TAACAGTCAA 
CCTGGAACTC CGAGTTCGTC GGCTATTGAC GATCCGGTGA CATCATCGAC TTATTCCGAC 
AAACAAAATA ATCAATCACC GGATTCAAGA CCGATTCAGG GCGGTACTGT TAGTACTAGC 
AATGTATCGC CATTGGATGG ATCACAATCA GATCCACCTA CACCTAACCC ACACACTCCA 
ACGCCTCCTA GGAATGATAA TATGCCATAT GCTGTCTACC AATCGCGTGA GGTTTGCAGT 
ACGAATTACC CACCGAAAAG TGACTGTTGT TCGAAAATGA ACACGCCGTA TATTTCGAGT 
ATAGTTACGA CGATGGCAAG TGGACGAACA GTTGGTAGTA ATACCATAAC GTCGGTTTTG 
GCCGGTCGTG CCAACACTGC CACAGTTTCA GTCAACAGTC CTGCTAACCT CCCAGCATCC 
ACAACCCCAA CAACAGTCAC ACAAACCGTT TCAAAATCCC CACTTGAAAT GGTCCAAAGC 
GTCGTCAGTA GCATACAAGT CCCTCAGTCT CAAACCAATC CCCAAATCTC TCCACCACAT 
ATCGTCAAAC ATTCCCCCAC CGGGTTACCT CCTGGCCATA TCCTTGTCTC AAGCGGCGGC 
CAGTTAATAA TGGCAAACAC CGGAAACAGC CAATCGGGCG TTATGCCACC CCCACCCCCC 
AAACTAGTCT CCAATCAAAC CGCAATGCCG CCAATGTCCG TCTCACCAAT GATCACAAAT 
GTCACTGCAG CGGTCAGTCA GGTCATACCG GCAGTAGCTC AGCAAGTTTT AGGCCAACAA 
ACCGTTTTAG TGAACGCCTT ACCCACACCT TTTGTCTTAC AAGGCGGAGT CACAATGACC 
ATGGACGGGT CCATGGTCGG CCAAAACATG CAATTACCTC AAATCGTCGC CGGAAATGTG 
ATACAACAAC AAATCCAACT GGATAACAGT GACCCACGAC GTACCACCGC GCTACTCTCA 
CCCGAAACCA AGAAAAAAGG AAAGAAACGA AAAATGTCAC AAACAGTGGC CAACATGCTT 
CACATAGCTG CCCAGCAAAA CTCCGGCGTT GTGATGTCAC AACAAGGCTT CCCGCAGCAA 
ATTCAAATGG CTCATAGTCC GCAAGGTCTA GCCACGGGCC CAGTGATGCA AGCCTTGACC 
ATAGTCCCCA GCAAAACAGG GGGACCCCCA CAGATCGTAA TGAACGGGCA ACCCATGACC 
AACACCAGTC CCCAACTCAT TGCAAACTCG CAACCGGCGC AACAAATCAA CCTCCTTCAG 
CCGGTGAATC TCATCAATGG CACCACCGGA GTGGTGCAAA ATTTCCCCAC GATTCAACAA 
TTCATAGTCC CGAATTTAGG CGGCATGGTT ATGAACCCGG ACGGCACCGC CACTATATTA 
CAGGACACTT CAAATATCGG CATGCAGTTA CAACTACAGA ACGTTAACGG ACAAAACGTC 
CTGACTCCGG TCCAAAACAA CGGAATGTTC AACGGGGGTC AGAGTATTCT AGCAGCGAGT 
CCGGCCGGCA TGGTCATTAG GGCCCCCACC TCGCAGGGGA AATTAATCCA GCAGCAGCAG 
CACAGTCCAG GTGCCCAGTT TTTGTCACCC AACGGGGGCC AGTTTGTCGT CAATGGGACA 
CAATTCAGCG GCCAGTTGAG CCCGCTTGTG GCTAGTGTGA GTCCGAGTCA GCAAGTTACC 
TTTGGGACGT CCACGCCGCA GATTAGACCA ACTCAGGGTC AACAAGAATT TATACAGTGT 
GGTCAAATGG GCCAGACTTT GATGGTTCCG TGTGCACCAA CGGTCGCCGT TTCCTCCTCG 
CAAAACACGA CTTTTGTCCA GCAAAACACT ACGATTGTTC AGCAGCAAAC CACGATGGTT 
TCGAATAGTC AACAGTTACA GAATTTCCAG AATAATGGAG GGAATCGAAC GACTGTGAAT 
GTGGATCAGA ATTTTATCAT CGCGAATGAT AATAAGCAAG TGCAGGCGCT TGTTCAGGTT 
CAGAGGGAGA GTCCCGGGAC GAATTATAGA CAGTCGGTGT CGACGCAAAC AGCGGTTAAT 
CAGAATGCAC AGACAGTGAC CACGTTTTGT CAGACGTCCA CGCTGTGTGC GGGGAGTCCC 
CCGGATACTA CAACGCATAG TCCGTTGGCG TCGGGAGGAC AGAGTCCTGC AACGGCCGAT 
ACGACCACAC ATACGGGAAG TACAGACGAT GGGCTGTCGC CAGCGCCGTC GAATTGTTCG 
GTGACGTGCG ATAGTGCAGG GAGACAGCCG GCTTGCACGA TGGCGATGGT GCACTGCATA 
TCCAGCAGCG AGCCCGATTC GGCTGATCTG AACACCCAAG GCTCTGACCA TGACTGGTCC 
CGAGCGTCGT CTATCCCTCA GCAAAAAAGC GACTTGTCGG AGATACATTT CCCCAAAAAA 
CAAAACACTC CGACCCACGT GGCTTACGCC GAATCGTCGA CAATGATGGC CGGTTTGCAT 
TTTATCGGCG ATCAAATGAA GGCCCAAGAG GCCGTCGTCA GTTCTCACAA TTTCGATAAA 
ATCGTACAGA AACGCAAAAG CAGCGATACT TTGCTCGTCA TGGAAACCCA TCACATGCAT 
TCGTCGCTTT TCGACGAGGA GGAAGATAAT GAGAAACGTG AAAAGTCAGA ACATGACTTT 
GTTGTTGGCG ATTTGGTTTG GGGGCCGGCT AAAGCAAGTC CAGCTTGGCC TGGCAAAATA 
ATACAAGTCG ACGGGAACGA TGTTTCGGTT AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC660029 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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