LOC659567 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC659567
Entrez Gene ID	659567
Full Name	proto-oncogene tyrosine-protein kinase ROS
Synonyms	sev,sevenless
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015985218.1	XP_015840704.1	LOW QUALITY PROTEIN: proto-oncogene tyrosine-protein kinase ROS

Pathways from BioSystems
Gene Ontology

The following LOC659567 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC659567 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg17020	XM_015985218.1 Latest version!	Tribolium castaneum proto-oncogene tyrosine-protein kinase ROS (LOC659567), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC659567 ( XM_015985218.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015840704.1 Tribolium castaneum proto-oncogene tyrosine-protein kinase ROS (LOC659567), mRNA.

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CloneID	OTg17020
Clone ID Related Accession (Same CDS sequence)	XM_015985218.1
Accession Version	XM_015985218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	LOW QUALITY PROTEIN: proto-oncogene tyrosine-protein kinase ROS
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450135.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCGATTAT TCTCAACTCC ACAAAGTGAA CATGTGATTT TTGGAAAAAT GCTAGTTTTG 
TGGCTTTTCG TGGTCATCGC CATCGGCCGG ACTCGTGCGA ATTTCGACCA CCAAGTGGAG 
TATCAGTGCA TCCAACACTG CCCTCTCCAG AATCGGACGG ATGCAGGCCA TTACGACTAC 
GCCTGTGATT ACGGATGTAA CATCAACCAG TGCAACAAAG GCTGCAAATT GTGGCGGAGA 
GCTTTGAACT CGTCGTGCCC CAAAGTCTGC AACGGTCCCA TGGACCGGCT CACCCCCAAA 
GCCCTCTACT GCGTAATGGG GTGTAACGAC GCCATGACCA AATACTTCAA CCAGTTGAAG 
TCGAAACTGC TGACCCCTCC GGCCCCAGCT CTGGTGGCTG ACACCCTCAG CGCCACTTCC 
CTCAGACTCG AGTGGAACTT CCCCGAGGCT AAGAGAGTCG GGCTGACTTA CCACGTCCAG 
TGGAAGTACG AAGAACTGAC AGCCACGTGG CACTACTGTC GTAATGTTAC GTGGTTTGAC 
AACGACACGG TTTTAATTGA AAACTTGCAA CCCTACACGA AATACAGATT TCGCATCGTC 
CTGATTTTGG GCCACCAGGA GAACCAGATC GTGTCGAACC CCAGCGTGGT CATAAGTACC 
CTGGCTTCGG GAATCCCAGT TTCGCCACCA AGTAGTGTAC GAGTGGCTCC CATTGATTCC 
ACACGTATAA GTGTGAGCTG GGAACCTGGC CCTTTCCCGC ACGGTCCCCT CCTCTCCTAT 
GTGCTCCAAA TCACTGATAA TCATCCAGAT GCCGAAGTAC CACCGGAAGT CAAGGATATT 
CCGGCCGAAA ATAACTTCTA CATGTTTAGA AACTTACGGC CAGCTCGTAA TTACACCATT 
TCCATCAAAA TGAGAAATGG GGTCGGGTCA GGGCCTTCGG CCAAAGTCAC AGTTTCTACA 
CCTTCCGAAT CTTTAGTAAA AGACACCCAA CAACCAGTTC TAATCCTTGG TACTGAGCAT 
GGAGTCGTAG AGGAGGGAAC TGACATCCTT GACGAGCCTG CCATCTTATA CAGCAGCAAA 
TTAAACATAA CAGGCATTGG GATACACATC AGCAAAAAGC TCATTTTCGT GTCAGATTCC 
AAAGGCCACA TTATCAAAAT GCCGCTATCT CGCAACAACA CGAACAATAT CACGTACATT 
TTGCCGCCGT TTCGCATCAA CCAGACGAAT TTCATGCCTC TAGACCTATC AATTGACTGG 
TTGAACGACC AGTTGTACAT TTTGGGCAAA ACGCAAAATG TTAAACACCC AATGTGGTTG 
ATAGCACGGT GCGATCTGGA TGGCCGTGGG CTTACCATCG CAGTGGCGGG TTTGACCACA 
CAACCGTACA ACATTGAAGT GGACCCATAC AATGGTTATC TTTTCTGGGG CATCCACGGC 
ACCGGTCTTT ACCGCTTAGA CATTTCCGAT TTTTCCAACG GAATCAGACA CGAAGTACAA 
CCGGTACTAA TCCTCAGCGA GAAGAAACTA AACATGAGTG CTTTCACAGT GGACCACACA 
AATTTCCGGC TTTTTGTCGC AAACGAGCGC GAAAAAACCA TAAATACAGT CTCGCTGGAT 
GGGAAGGAAA TAATCAACGT GCGGCCAAAC GTTATCAAGT CGAAACTCGA AAACGTCGTG 
TCTTTAGCCA CCGCTAACAA AAAATTCTAT TGGACGAACG GTGACGATAT TTTTTACGAA 
GAGTACCACA ATTCCGTCTA CTTCCACAAC TCGTACCCCG ACCTATCATT GTCCACAAAA 
TCGTACAAGA AAGTTATAAT TAACTTGCAA AGTTCGCAAC CTATCCCCAG CCCTGTGAAC 
CCCCCAACCA ACGTCCAGGC AATTTTTGGG AGCAACATAG CCAAAACAAC GTGGCTGCCC 
CCTCATTTGT TGGGGATTCA GGGCAAGGGT GCTTGGCAGA ACTGGTCGTA CGAAGTCGCG 
ATTAAAGAAG TGGGTTCGCA AAAGTTCATC TCGCACCGAA ATATCAACAC CACGTCTTAC 
AAAATCTCAG GCTTGAGGGA AAACACGGAG TATGTTATCA AAGCTGCGGC TTACACCAGG 
TCGGGGCAAG GGCCCTGGTC GTCCGAATTC CGGGGATTCA CCTTAAGTCG CTCGAAAAAC 
CCCATGATTT ATTGGTCGGC TGCCAAAGGC CTGCTGCGGA CTGACGCCGC TGGGGAAAAT 
GTCGAGACGT TGATTCACAA AAGCAGGATT AAAGGCTCGG TTTTTATCGA CATGACGTGG 
TACCAAGACT TGGTTTATTT GGTCACGAAT GACTCGCATG TCTATTGGTA CGACACCAGG 
AGTCATAGTC AGGGACAGTT GATTGGTATT GACTCAGTTG GCAGTATTGC TGTTGACTGG 
ATCGGGAAAA AACTCTACTG GTCGAACCCT AAACGACAAA TGATTATCAG AGGGAACTTG 
AATGGTACCC AGCAGGAACC ACTGCCAATT CTCACACTGG CCAAGGAACT GAACATTGAT 
TCAGTCAAAG CTTATCTGTA CTGGTCCACA GGGTTTGCAG TTCGCTGTGC ACACCTTAAC 
GGGGAAAACT TGATAGAGTA CCACCCGGTA CAGCTATTTT CCGGAAGACA AGTGATGGGC 
CTAACCCTCG ACATGCACAA CAAATTCGTC TACTGGATCG TCCGAGGTCT GGAAGGCTCG 
AATTTATTCC GGGCCCCGAT GGCCGGCTAT TCCGAAAAAA TCACCGAAAA TTTCAAAATT 
ACGAGTTTGC AAAAACCCAA CATGCAGGGC CCACTTTGCT ACTTCCACAA GCGTCTCTTG 
TGGCTCCAAG ACAACAAAAA CGCGGCTATT AGTGACCTCC AAGGCAACAA CATTGCCACA 
ATCAGCGGAA AAAGCATGTC TGATTTAACC CTAGTCTACG TGGTTGATTC CTCATTGCAC 
GTTATGCCCG ACATTCCCGA AATTGTGGTA ATCCCCGAAA CGGTAGACAA AAATTCGGTC 
CGAGTGATCG GTTCTTTAAA TTCGTTCAAT GTGACTTGGA ACCCAGTCAA GAACGTCAAC 
TACGGGCAAG TGTTTTACGA AGTCAGTATT GACAGTCTTT TACGGAACGA TTCTGCAATT 
GTTACGAATT CGACTTCAGT GAGGTATTGG CAACAAGTGG CCCCTTTTAC CAAACTCCAA 
GTGGCAATCC GGGCGTTCAC TTATTGGGGC ACTTCGCCCC AAATCCGCGC CAAAGTCTAC 
TCCCCGGCCT CGACCCCTTC AGTCCCCACA AATCTCCGAG CTTACGTAAC GCACCACAGC 
AGCGGCACCA ACGTCACTAT CACCGTCCGC TGGGGCCCTC CCTTGTTCCC CAACGGGGTG 
CTCAAAGGCT ACAAGATACA CTGCTGGTAC TTGAACGCCG CGCAACGAAG AGACGTGTGC 
CGGGACTTGG TCAAAAAACC GGAGGAGTTC CAGATTGATT TCAAGCCACC ACACGACCAC 
ATCTACTACT TCAACGTGCA AGCTTTCAGC GAAATGGGCG ACGGGGATTT GTCCGACCCC 
ATCCAGGTCA ACTCCAGCCA AGAACTACCA TTGCCACGGC TCTTGGTGGC GTCGGAAGAC 
TCGATTTTCA TCCAAGACAT TGACTCGGAT CGGAACGAGT CGCTCTTACA CGGGATTGTA 
ACACCGACGG AGATCGGCTA TCTGATCAAA GAATCGAAAC TGTTCTGGAT CGACCAGTTG 
CAAAACCTCA TGATGTACGA TATGTTGAAA GGGAATAAGA CTAAAATCAC GAGCTTGCGG 
AACAATGTCA CGGGCGTGAC CGTTGATTGG CTGGAGCGGA GCATTTACTA CGCCCAGAGC 
GAGAATTGGG ACTCGGGCTC GAGCGTTTTC AAGCTTGATT TAAACTATGT TGATCGGGGG 
ATTGTCAAAA TGAGCAAAGT TTTCTTCACG CCGGCTAGTA TCAGTAAAAT CGAGGTTTCG 
CCCTACACCA GGAAGCTCTA CTGGGTGGAA AAATCCAAGT TGATGGTTTG CAACACCGAT 
GGGAGCGACA AAAGCGAGTT TTTTAACAAG AACAAGTACA CCAAACGATC CACACTTGGT 
GGGGGTTGCA ATTGCCCGGT GGACGTCAAT ATTGAGAAAA CCTACACTTT GGACCACTCG 
ACTGAGACTA AACCATTGCT AGTTTTCGTA GAGTCTGACA CTCAGAATGT GGTGTCGGCC 
GACAAAAACG GCTGTTCCTG CAGTGTTATT GCAAACAACT CACTTGGTGT CACACTCCCG 
CTTGATCGTT TGAAATCGGA CTTTGGTACT TTGTATTGGA CAAATTCGCG CCTTCTTCAC 
GCACTGAGGA AGAAAGAGGC GAATTTGGTC ACGAAGGAGG TGAACGGTCG CGATATCCTC 
ATTTTCGGGC CGCATATGCA ACCCTTCCCC CCTAGCGAGT GTCTGAGACC ACGGCAGTTA 
GCCAACATTA GAGTGACTCT AAAGGAAAAA ACTGCAACTT CCTTGACTTT GGTTATGCCA 
GAGGTTGGGC AAATCTGCGC CGACACTTCG ATGCCCACGG TTGAGTACCG AATTTATTAC 
GCGGAGAAAA TCGACAACAA CACGTGTGAC CTCACTTGCA ACTTCTCCGC AACTTTCGAT 
AAAGAATTCA CGATCAAAAG TTTGAAACCG TTCACTCACT ACGTGGTTGC GGTCGCTGTT 
AGCAATTACT ACACCGAGGA AGAGGAAATA GTCCTCGGAC CCTCTACAGT TTTTCAGACC 
GCAGTCGGAG CACCTTCGCC CCCACAAAAC GTCACAGTTA AAGTGCTGAA CCCGACAATG 
GCCCAAGTGT CGTGGTCGCC CCCTCAGCAC CCCAACGGTG TGACAGTTCA CTACGAAATC 
TACTGCCAGA CCGAACGGAC CCTTTCGGGT GTTCGCCAAA AAGAACAGTC GAATGTTGAG 
CAACAATTTA CCCCAAACTC CCACTTTCTC ACGATCGTCC TCAACAAACT CTCCCCTAAT 
GAGACTTACA CGATCTGGGT GAAGGCATAC AGTGAGACGA ACGAGACGTC CAGTGACAGT 
GACAAAGTGC AAATAAACAC GTACCCCGAA CCCAGTGACT TAATTCTAGT GAACCGGACC 
GCGTACATGT TGACTGTGAC ATGGGACGTG TGTGCCAACA TCGACCACTA CAACGTGCAA 
ATGTCCCCAG TCACGTCCAA CGACTGGCGG AACGTGAGCG AATCTGGCGA AGGTATTTTC 
GCCAGTGAGG AGAATTTGCA ACCAAAGACA CAGTACAAGT TCCGGTTGTC CCTCTCCTAT 
TACAATTACC CCGAGGAGTA CATTTGGCCC CAAGACTCTC GCTTTGTTTA CGAAACGCTT 
GGAGACAAGC CCTCGCCGCC TGGCACCCCC AAAATCCAGT ACGTCAAGCC CAACATTTAC 
AAAGTCTGGT GGGAGGAAGC CAAGGATAAC GGAGCACCGA TCGAATTGTA CAAACTCGAA 
GGTTTGGAAC TCCCGAATTA CCGCACGAAG AGAAGTACAA ACCGTACCGC TTTCTTCTAC 
ACCGCGCCTT CGGTCGAGGA GGAAGAGCGG GAATGGCAGC AGTTTTACAA CGGGACTGAA 
ACGTCCTGGA TTATCAACGG CTTGAGCGAG AAATACAAGT ATGAGTTTAG GGTTTTGGCC 
TTGAATGCGT ACGGGTGGAG CGACCCGAGT GACTCTAGCA CGGCTTTTGA CCTGAACGAA 
GCCGCCAGAA TGGCCGAGAA GCAAAGCCCG ATGACGCTGA TTGCCATTGC TACTTTTATC 
CCAGTCTCGG TTTTCCTGAT CATTTTGTTG TGCATTGTGT GCCGGAAATA CGGGAAGCCG 
AAGAAGGTGC AAGTCGTGTC AATACCACGG GGGCCTGACG TCGAGTTGGC GACACTGAGG 
GAACTCCCCC GGAGGGGCAT CCACAACACC AACGTGTTGT ACGTCTCCAC ACAGCCCACG 
AGTGAGGAGA TGACGCTTTT GCCACATATT CGGAGGGACC AAATTACTTT GATGAAGTTT 
CTGGGAAGTG GGGCTTTTGG GGAGGTTTTT GAAGGGAAAG CCAGGGGGAT TTCTAACAGT 
TCAGGGGATA CTAAAGTAGC AGTTAAGACT TTGAGGAAGT GTGCCTCCGA TCAGGAGAAA 
GCCGAGTTCT TGCAAGAAGC GCAGCTTATG AGTAACTTCA AGCACGAACA TATTTTGCAG 
CTTTTGGGGG TTTGTTTAGA TAATGATCCG CATTTTATCA TCATGGAGTT GATGCAAGGG 
GGCGATTTGT TGACTTATCT GCGCTCCTCA AGGAACCCCA ACACCGACAC CCCTTCCCTC 
TCCTTGATCG AGCTCCTGAA AATGTGCGTG GACGTGGCTA AGGGCTGCCG CTACCTGGAA 
GAGATGCACT TCGTGCACAG AGACTTGGCC TGTCGTAACT GTCTCGTTTC GTCAAAAGAC 
CCCGACCGAA TTGTGAAAAT CGGCGACTTC GGCCTGGCCC GGGACATCTA CAAGAACGAC 
TACTACAGGA AAGAGGGCGA AGGCCTGCTG CCGGTCCGCT GGATGGCCCC CGAGTCGCTG 
GTCGACGGAG TCTTCACGAG CCAGTCGGAC GTCTGGGCCT TCGGGGTCCT CCTGTGGGAA 
ATAATGACCT TGGGGCAACA GCCGTACCCC GCCCGAAACA ACCTCGAAGT GCTGCATTAC 
GTACGGCGCG GGGGCCGGCT GGGGAAACCC ACAGATTGTC CAGAAACTTT GCACAATTTG 
ATGCTGAAGT GTTGGGAATT CGAGGCCGAT AAGAGGCCCA CTTTCAAGTA CTGCTTGGAC 
GTGCTGGACA ACCTGCACTT GCAGAACCTG CGGAGCCCCA CGACGGGGGC CCACGAGGGC 
CAGTACATAA GCACCGTGCA GGAATATTTT ATTCAAGGTA TTTCCAATGA GGCGTATTTT 
CGCGATGAGA ACTTTTCAGG TGACATTGAA GATGATTCCA ATAAGGAGAA GACACCGTTT 
CTGGCCTCGG AAGCCGCTTC TGCAATTCCC AAGTATCTGG AATTGTTGTA TGAACCAGAG 
ACGTGCCCGC CAAATGACGG CTATGAAATA CCGAACCAAA TGCTGCAAAA TGAAAGTACA 
ACTAAACGAA GTTCCTCAGT AGTGTCCATA TCGAGTAATG GGAATGTGCC AGACAAGAAA 
ACACAAAACG GCTCTATTTG TAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015840704.1
CDS	81..7247
Translation	MRLFSTPQSEHVIFGKMLVLWLFVVIAIGRTRANFDHQVEYQCIQHCPLQNRTDAGHYDYACDYGCNINQCNKGCKLWRRALNSSCPKVCNGPMDRLTPKALYCVMGCNDAMTKYFNQLKSKLLTPPAPALVADTLSATSLRLEWNFPEAKRVGLTYHVQWKYEELTATWHYCRNVTWFDNDTVLIENLQPYTKYRFRIVLILGHQENQIVSNPSVVISTLASGIPVSPPSSVRVAPIDSTRISVSWEPGPFPHGPLLSYVLQITDNHPDAEVPPEVKDIPAENNFYMFRNLRPARNYTISIKMRNGVGSGPSAKVTVSTPSESLVKDTQQPVLILGTEHGVVEEGTDILDEPAILYSSKLNITGIGIHISKKLIFVSDSKGHIIKMPLSRNNTNNITYILPPFRINQTNFMPLDLSIDWLNDQLYILGKTQNVKHPMWLIARCDLDGRGLTIAVAGLTTQPYNIEVDPYNGYLFWGIHGTGLYRLDISDFSNGIRHEVQPVLILSEKKLNMSAFTVDHTNFRLFVANEREKTINTVSLDGKEIINVRPNVIKSKLENVVSLATANKKFYWTNGDDIFYEEYHNSVYFHNSYPDLSLSTKSYKKVIINLQSSQPIPSPVNPPTNVQAIFGSNIAKTTWLPPHLLGIQGKGAWQNWSYEVAIKEVGSQKFISHRNINTTSYKISGLRENTEYVIKAAAYTRSGQGPWSSEFRGFTLSRSKNPMIYWSAAKGLLRTDAAGENVETLIHKSRIKGSVFIDMTWYQDLVYLVTNDSHVYWYDTRSHSQGQLIGIDSVGSIAVDWIGKKLYWSNPKRQMIIRGNLNGTQQEPLPILTLAKELNIDSVKAYLYWSTGFAVRCAHLNGENLIEYHPVQLFSGRQVMGLTLDMHNKFVYWIVRGLEGSNLFRAPMAGYSEKITENFKITSLQKPNMQGPLCYFHKRLLWLQDNKNAAISDLQGNNIATISGKSMSDLTLVYVVDSSLHVMPDIPEIVVIPETVDKNSVRVIGSLNSFNVTWNPVKNVNYGQVFYEVSIDSLLRNDSAIVTNSTSVRYWQQVAPFTKLQVAIRAFTYWGTSPQIRAKVYSPASTPSVPTNLRAYVTHHSSGTNVTITVRWGPPLFPNGVLKGYKIHCWYLNAAQRRDVCRDLVKKPEEFQIDFKPPHDHIYYFNVQAFSEMGDGDLSDPIQVNSSQELPLPRLLVASEDSIFIQDIDSDRNESLLHGIVTPTEIGYLIKESKLFWIDQLQNLMMYDMLKGNKTKITSLRNNVTGVTVDWLERSIYYAQSENWDSGSSVFKLDLNYVDRGIVKMSKVFFTPASISKIEVSPYTRKLYWVEKSKLMVCNTDGSDKSEFFNKNKYTKRSTLGGGCNCPVDVNIEKTYTLDHSTETKPLLVFVESDTQNVVSADKNGCSCSVIANNSLGVTLPLDRLKSDFGTLYWTNSRLLHALRKKEANLVTKEVNGRDILIFGPHMQPFPPSECLRPRQLANIRVTLKEKTATSLTLVMPEVGQICADTSMPTVEYRIYYAEKIDNNTCDLTCNFSATFDKEFTIKSLKPFTHYVVAVAVSNYYTEEEEIVLGPSTVFQTAVGAPSPPQNVTVKVLNPTMAQVSWSPPQHPNGVTVHYEIYCQTERTLSGVRQKEQSNVEQQFTPNSHFLTIVLNKLSPNETYTIWVKAYSETNETSSDSDKVQINTYPEPSDLILVNRTAYMLTVTWDVCANIDHYNVQMSPVTSNDWRNVSESGEGIFASEENLQPKTQYKFRLSLSYYNYPEEYIWPQDSRFVYETLGDKPSPPGTPKIQYVKPNIYKVWWEEAKDNGAPIELYKLEGLELPNYRTKRSTNRTAFFYTAPSVEEEEREWQQFYNGTETSWIINGLSEKYKYEFRVLALNAYGWSDPSDSSTAFDLNEAARMAEKQSPMTLIAIATFIPVSVFLIILLCIVCRKYGKPKKVQVVSIPRGPDVELATLRELPRRGIHNTNVLYVSTQPTSEEMTLLPHIRRDQITLMKFLGSGAFGEVFEGKARGISNSSGDTKVAVKTLRKCASDQEKAEFLQEAQLMSNFKHEHILQLLGVCLDNDPHFIIMELMQGGDLLTYLRSSRNPNTDTPSLSLIELLKMCVDVAKGCRYLEEMHFVHRDLACRNCLVSSKDPDRIVKIGDFGLARDIYKNDYYRKEGEGLLPVRWMAPESLVDGVFTSQSDVWAFGVLLWEIMTLGQQPYPARNNLEVLHYVRRGGRLGKPTDCPETLHNLMLKCWEFEADKRPTFKYCLDVLDNLHLQNLRSPTTGAHEGQYISTVQEYFIQGISNEAYFRDENFSGDIEDDSNKEKTPFLASEAASAIPKYLELLYEPETCPPNDGYEIPNQMLQNESTTKRSSSVVSISSNGNVPDKKTQNGSICK

Target ORF information:

RefSeq Version	XM_015985218.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum proto-oncogene tyrosine-protein kinase ROS (LOC659567), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015985218.1

ORF Insert Sequence:

ATGCGATTAT TCTCAACTCC ACAAAGTGAA CATGTGATTT TTGGAAAAAT GCTAGTTTTG 
TGGCTTTTCG TGGTCATCGC CATCGGCCGG ACTCGTGCGA ATTTCGACCA CCAAGTGGAG 
TATCAGTGCA TCCAACACTG CCCTCTCCAG AATCGGACGG ATGCAGGCCA TTACGACTAC 
GCCTGTGATT ACGGATGTAA CATCAACCAG TGCAACAAAG GCTGCAAATT GTGGCGGAGA 
GCTTTGAACT CGTCGTGCCC CAAAGTCTGC AACGGTCCCA TGGACCGGCT CACCCCCAAA 
GCCCTCTACT GCGTAATGGG GTGTAACGAC GCCATGACCA AATACTTCAA CCAGTTGAAG 
TCGAAACTGC TGACCCCTCC GGCCCCAGCT CTGGTGGCTG ACACCCTCAG CGCCACTTCC 
CTCAGACTCG AGTGGAACTT CCCCGAGGCT AAGAGAGTCG GGCTGACTTA CCACGTCCAG 
TGGAAGTACG AAGAACTGAC AGCCACGTGG CACTACTGTC GTAATGTTAC GTGGTTTGAC 
AACGACACGG TTTTAATTGA AAACTTGCAA CCCTACACGA AATACAGATT TCGCATCGTC 
CTGATTTTGG GCCACCAGGA GAACCAGATC GTGTCGAACC CCAGCGTGGT CATAAGTACC 
CTGGCTTCGG GAATCCCAGT TTCGCCACCA AGTAGTGTAC GAGTGGCTCC CATTGATTCC 
ACACGTATAA GTGTGAGCTG GGAACCTGGC CCTTTCCCGC ACGGTCCCCT CCTCTCCTAT 
GTGCTCCAAA TCACTGATAA TCATCCAGAT GCCGAAGTAC CACCGGAAGT CAAGGATATT 
CCGGCCGAAA ATAACTTCTA CATGTTTAGA AACTTACGGC CAGCTCGTAA TTACACCATT 
TCCATCAAAA TGAGAAATGG GGTCGGGTCA GGGCCTTCGG CCAAAGTCAC AGTTTCTACA 
CCTTCCGAAT CTTTAGTAAA AGACACCCAA CAACCAGTTC TAATCCTTGG TACTGAGCAT 
GGAGTCGTAG AGGAGGGAAC TGACATCCTT GACGAGCCTG CCATCTTATA CAGCAGCAAA 
TTAAACATAA CAGGCATTGG GATACACATC AGCAAAAAGC TCATTTTCGT GTCAGATTCC 
AAAGGCCACA TTATCAAAAT GCCGCTATCT CGCAACAACA CGAACAATAT CACGTACATT 
TTGCCGCCGT TTCGCATCAA CCAGACGAAT TTCATGCCTC TAGACCTATC AATTGACTGG 
TTGAACGACC AGTTGTACAT TTTGGGCAAA ACGCAAAATG TTAAACACCC AATGTGGTTG 
ATAGCACGGT GCGATCTGGA TGGCCGTGGG CTTACCATCG CAGTGGCGGG TTTGACCACA 
CAACCGTACA ACATTGAAGT GGACCCATAC AATGGTTATC TTTTCTGGGG CATCCACGGC 
ACCGGTCTTT ACCGCTTAGA CATTTCCGAT TTTTCCAACG GAATCAGACA CGAAGTACAA 
CCGGTACTAA TCCTCAGCGA GAAGAAACTA AACATGAGTG CTTTCACAGT GGACCACACA 
AATTTCCGGC TTTTTGTCGC AAACGAGCGC GAAAAAACCA TAAATACAGT CTCGCTGGAT 
GGGAAGGAAA TAATCAACGT GCGGCCAAAC GTTATCAAGT CGAAACTCGA AAACGTCGTG 
TCTTTAGCCA CCGCTAACAA AAAATTCTAT TGGACGAACG GTGACGATAT TTTTTACGAA 
GAGTACCACA ATTCCGTCTA CTTCCACAAC TCGTACCCCG ACCTATCATT GTCCACAAAA 
TCGTACAAGA AAGTTATAAT TAACTTGCAA AGTTCGCAAC CTATCCCCAG CCCTGTGAAC 
CCCCCAACCA ACGTCCAGGC AATTTTTGGG AGCAACATAG CCAAAACAAC GTGGCTGCCC 
CCTCATTTGT TGGGGATTCA GGGCAAGGGT GCTTGGCAGA ACTGGTCGTA CGAAGTCGCG 
ATTAAAGAAG TGGGTTCGCA AAAGTTCATC TCGCACCGAA ATATCAACAC CACGTCTTAC 
AAAATCTCAG GCTTGAGGGA AAACACGGAG TATGTTATCA AAGCTGCGGC TTACACCAGG 
TCGGGGCAAG GGCCCTGGTC GTCCGAATTC CGGGGATTCA CCTTAAGTCG CTCGAAAAAC 
CCCATGATTT ATTGGTCGGC TGCCAAAGGC CTGCTGCGGA CTGACGCCGC TGGGGAAAAT 
GTCGAGACGT TGATTCACAA AAGCAGGATT AAAGGCTCGG TTTTTATCGA CATGACGTGG 
TACCAAGACT TGGTTTATTT GGTCACGAAT GACTCGCATG TCTATTGGTA CGACACCAGG 
AGTCATAGTC AGGGACAGTT GATTGGTATT GACTCAGTTG GCAGTATTGC TGTTGACTGG 
ATCGGGAAAA AACTCTACTG GTCGAACCCT AAACGACAAA TGATTATCAG AGGGAACTTG 
AATGGTACCC AGCAGGAACC ACTGCCAATT CTCACACTGG CCAAGGAACT GAACATTGAT 
TCAGTCAAAG CTTATCTGTA CTGGTCCACA GGGTTTGCAG TTCGCTGTGC ACACCTTAAC 
GGGGAAAACT TGATAGAGTA CCACCCGGTA CAGCTATTTT CCGGAAGACA AGTGATGGGC 
CTAACCCTCG ACATGCACAA CAAATTCGTC TACTGGATCG TCCGAGGTCT GGAAGGCTCG 
AATTTATTCC GGGCCCCGAT GGCCGGCTAT TCCGAAAAAA TCACCGAAAA TTTCAAAATT 
ACGAGTTTGC AAAAACCCAA CATGCAGGGC CCACTTTGCT ACTTCCACAA GCGTCTCTTG 
TGGCTCCAAG ACAACAAAAA CGCGGCTATT AGTGACCTCC AAGGCAACAA CATTGCCACA 
ATCAGCGGAA AAAGCATGTC TGATTTAACC CTAGTCTACG TGGTTGATTC CTCATTGCAC 
GTTATGCCCG ACATTCCCGA AATTGTGGTA ATCCCCGAAA CGGTAGACAA AAATTCGGTC 
CGAGTGATCG GTTCTTTAAA TTCGTTCAAT GTGACTTGGA ACCCAGTCAA GAACGTCAAC 
TACGGGCAAG TGTTTTACGA AGTCAGTATT GACAGTCTTT TACGGAACGA TTCTGCAATT 
GTTACGAATT CGACTTCAGT GAGGTATTGG CAACAAGTGG CCCCTTTTAC CAAACTCCAA 
GTGGCAATCC GGGCGTTCAC TTATTGGGGC ACTTCGCCCC AAATCCGCGC CAAAGTCTAC 
TCCCCGGCCT CGACCCCTTC AGTCCCCACA AATCTCCGAG CTTACGTAAC GCACCACAGC 
AGCGGCACCA ACGTCACTAT CACCGTCCGC TGGGGCCCTC CCTTGTTCCC CAACGGGGTG 
CTCAAAGGCT ACAAGATACA CTGCTGGTAC TTGAACGCCG CGCAACGAAG AGACGTGTGC 
CGGGACTTGG TCAAAAAACC GGAGGAGTTC CAGATTGATT TCAAGCCACC ACACGACCAC 
ATCTACTACT TCAACGTGCA AGCTTTCAGC GAAATGGGCG ACGGGGATTT GTCCGACCCC 
ATCCAGGTCA ACTCCAGCCA AGAACTACCA TTGCCACGGC TCTTGGTGGC GTCGGAAGAC 
TCGATTTTCA TCCAAGACAT TGACTCGGAT CGGAACGAGT CGCTCTTACA CGGGATTGTA 
ACACCGACGG AGATCGGCTA TCTGATCAAA GAATCGAAAC TGTTCTGGAT CGACCAGTTG 
CAAAACCTCA TGATGTACGA TATGTTGAAA GGGAATAAGA CTAAAATCAC GAGCTTGCGG 
AACAATGTCA CGGGCGTGAC CGTTGATTGG CTGGAGCGGA GCATTTACTA CGCCCAGAGC 
GAGAATTGGG ACTCGGGCTC GAGCGTTTTC AAGCTTGATT TAAACTATGT TGATCGGGGG 
ATTGTCAAAA TGAGCAAAGT TTTCTTCACG CCGGCTAGTA TCAGTAAAAT CGAGGTTTCG 
CCCTACACCA GGAAGCTCTA CTGGGTGGAA AAATCCAAGT TGATGGTTTG CAACACCGAT 
GGGAGCGACA AAAGCGAGTT TTTTAACAAG AACAAGTACA CCAAACGATC CACACTTGGT 
GGGGGTTGCA ATTGCCCGGT GGACGTCAAT ATTGAGAAAA CCTACACTTT GGACCACTCG 
ACTGAGACTA AACCATTGCT AGTTTTCGTA GAGTCTGACA CTCAGAATGT GGTGTCGGCC 
GACAAAAACG GCTGTTCCTG CAGTGTTATT GCAAACAACT CACTTGGTGT CACACTCCCG 
CTTGATCGTT TGAAATCGGA CTTTGGTACT TTGTATTGGA CAAATTCGCG CCTTCTTCAC 
GCACTGAGGA AGAAAGAGGC GAATTTGGTC ACGAAGGAGG TGAACGGTCG CGATATCCTC 
ATTTTCGGGC CGCATATGCA ACCCTTCCCC CCTAGCGAGT GTCTGAGACC ACGGCAGTTA 
GCCAACATTA GAGTGACTCT AAAGGAAAAA ACTGCAACTT CCTTGACTTT GGTTATGCCA 
GAGGTTGGGC AAATCTGCGC CGACACTTCG ATGCCCACGG TTGAGTACCG AATTTATTAC 
GCGGAGAAAA TCGACAACAA CACGTGTGAC CTCACTTGCA ACTTCTCCGC AACTTTCGAT 
AAAGAATTCA CGATCAAAAG TTTGAAACCG TTCACTCACT ACGTGGTTGC GGTCGCTGTT 
AGCAATTACT ACACCGAGGA AGAGGAAATA GTCCTCGGAC CCTCTACAGT TTTTCAGACC 
GCAGTCGGAG CACCTTCGCC CCCACAAAAC GTCACAGTTA AAGTGCTGAA CCCGACAATG 
GCCCAAGTGT CGTGGTCGCC CCCTCAGCAC CCCAACGGTG TGACAGTTCA CTACGAAATC 
TACTGCCAGA CCGAACGGAC CCTTTCGGGT GTTCGCCAAA AAGAACAGTC GAATGTTGAG 
CAACAATTTA CCCCAAACTC CCACTTTCTC ACGATCGTCC TCAACAAACT CTCCCCTAAT 
GAGACTTACA CGATCTGGGT GAAGGCATAC AGTGAGACGA ACGAGACGTC CAGTGACAGT 
GACAAAGTGC AAATAAACAC GTACCCCGAA CCCAGTGACT TAATTCTAGT GAACCGGACC 
GCGTACATGT TGACTGTGAC ATGGGACGTG TGTGCCAACA TCGACCACTA CAACGTGCAA 
ATGTCCCCAG TCACGTCCAA CGACTGGCGG AACGTGAGCG AATCTGGCGA AGGTATTTTC 
GCCAGTGAGG AGAATTTGCA ACCAAAGACA CAGTACAAGT TCCGGTTGTC CCTCTCCTAT 
TACAATTACC CCGAGGAGTA CATTTGGCCC CAAGACTCTC GCTTTGTTTA CGAAACGCTT 
GGAGACAAGC CCTCGCCGCC TGGCACCCCC AAAATCCAGT ACGTCAAGCC CAACATTTAC 
AAAGTCTGGT GGGAGGAAGC CAAGGATAAC GGAGCACCGA TCGAATTGTA CAAACTCGAA 
GGTTTGGAAC TCCCGAATTA CCGCACGAAG AGAAGTACAA ACCGTACCGC TTTCTTCTAC 
ACCGCGCCTT CGGTCGAGGA GGAAGAGCGG GAATGGCAGC AGTTTTACAA CGGGACTGAA 
ACGTCCTGGA TTATCAACGG CTTGAGCGAG AAATACAAGT ATGAGTTTAG GGTTTTGGCC 
TTGAATGCGT ACGGGTGGAG CGACCCGAGT GACTCTAGCA CGGCTTTTGA CCTGAACGAA 
GCCGCCAGAA TGGCCGAGAA GCAAAGCCCG ATGACGCTGA TTGCCATTGC TACTTTTATC 
CCAGTCTCGG TTTTCCTGAT CATTTTGTTG TGCATTGTGT GCCGGAAATA CGGGAAGCCG 
AAGAAGGTGC AAGTCGTGTC AATACCACGG GGGCCTGACG TCGAGTTGGC GACACTGAGG 
GAACTCCCCC GGAGGGGCAT CCACAACACC AACGTGTTGT ACGTCTCCAC ACAGCCCACG 
AGTGAGGAGA TGACGCTTTT GCCACATATT CGGAGGGACC AAATTACTTT GATGAAGTTT 
CTGGGAAGTG GGGCTTTTGG GGAGGTTTTT GAAGGGAAAG CCAGGGGGAT TTCTAACAGT 
TCAGGGGATA CTAAAGTAGC AGTTAAGACT TTGAGGAAGT GTGCCTCCGA TCAGGAGAAA 
GCCGAGTTCT TGCAAGAAGC GCAGCTTATG AGTAACTTCA AGCACGAACA TATTTTGCAG 
CTTTTGGGGG TTTGTTTAGA TAATGATCCG CATTTTATCA TCATGGAGTT GATGCAAGGG 
GGCGATTTGT TGACTTATCT GCGCTCCTCA AGGAACCCCA ACACCGACAC CCCTTCCCTC 
TCCTTGATCG AGCTCCTGAA AATGTGCGTG GACGTGGCTA AGGGCTGCCG CTACCTGGAA 
GAGATGCACT TCGTGCACAG AGACTTGGCC TGTCGTAACT GTCTCGTTTC GTCAAAAGAC 
CCCGACCGAA TTGTGAAAAT CGGCGACTTC GGCCTGGCCC GGGACATCTA CAAGAACGAC 
TACTACAGGA AAGAGGGCGA AGGCCTGCTG CCGGTCCGCT GGATGGCCCC CGAGTCGCTG 
GTCGACGGAG TCTTCACGAG CCAGTCGGAC GTCTGGGCCT TCGGGGTCCT CCTGTGGGAA 
ATAATGACCT TGGGGCAACA GCCGTACCCC GCCCGAAACA ACCTCGAAGT GCTGCATTAC 
GTACGGCGCG GGGGCCGGCT GGGGAAACCC ACAGATTGTC CAGAAACTTT GCACAATTTG 
ATGCTGAAGT GTTGGGAATT CGAGGCCGAT AAGAGGCCCA CTTTCAAGTA CTGCTTGGAC 
GTGCTGGACA ACCTGCACTT GCAGAACCTG CGGAGCCCCA CGACGGGGGC CCACGAGGGC 
CAGTACATAA GCACCGTGCA GGAATATTTT ATTCAAGGTA TTTCCAATGA GGCGTATTTT 
CGCGATGAGA ACTTTTCAGG TGACATTGAA GATGATTCCA ATAAGGAGAA GACACCGTTT 
CTGGCCTCGG AAGCCGCTTC TGCAATTCCC AAGTATCTGG AATTGTTGTA TGAACCAGAG 
ACGTGCCCGC CAAATGACGG CTATGAAATA CCGAACCAAA TGCTGCAAAA TGAAAGTACA 
ACTAAACGAA GTTCCTCAGT AGTGTCCATA TCGAGTAATG GGAATGTGCC AGACAAGAAA 
ACACAAAACG GCTCTATTTG TAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC659567 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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