LOC658843 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC658843
Entrez Gene ID	658843
Full Name	histone-lysine N-methyltransferase trithorax
Synonyms	trithorax,trx
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008202486.2	XP_008200708.1	histone-lysine N-methyltransferase trithorax

Pathways from BioSystems
Gene Ontology

The following LOC658843 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC658843 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg00740	XM_008202486.2 Latest version!	Tribolium castaneum histone-lysine N-methyltransferase trithorax (LOC658843), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC658843 ( XM_008202486.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008200708.1 Tribolium castaneum histone-lysine N-methyltransferase trithorax (LOC658843), mRNA.

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CloneID	OTg00740
Clone ID Related Accession (Same CDS sequence)	XM_008202486.2
Accession Version	XM_008202486.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	histone-lysine N-methyltransferase trithorax
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008202486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGTT CAAAATTTCC CGGAAAACCA TCGAAACATG TTAATAGGAA AAGAATTAAT 
GTTCTTCCAC CCACCTCGGA GGGAATTATT GAATCTAGCA GGTCTATGAC AGTTGAAACT 
GTTGCAGAAA ACAAACAGGC CGAAAATGAT GTAGAAGAAA GCAGTGAAAG TGTAAATTTA 
AACAATACTA TAAATACAAA AGTGTCATTG AGAAGAAGAC TGAACCGAAA AATGCAAAGT 
TCGAAAGCAT TGATGAGCAA TAATTCGTCT AAACGTGGTC TAATATCAAA ACTCTGTAAT 
AAAAATAGTG TTTCCTGCAA ACTCTCGGTT AAAAGCAAAT TAAATTGCAA AGCATCAAGG 
AATCGCAAAT CAGTGCATGC ATACTCTCCA ATAAAAAATT TCCGCCAGTG TCGAAGTGGA 
ATAACAAAAG AAAGTAATAA TCTCGTTGGA AAATTCGTAT TACCCACAAG AAGTGTCCAT 
TCATCGCGTG TGATAAAACC AAACAAAAGA TTCATAAACG TGGATTTGAG TGACACATTA 
ACCCTAAAAA AGCGCGTCAT TAACAAAAGA CATTGTATAA AACAAAGCGA AAATCCGTCG 
AAAACCAACA AAAGTGACAG TGCATCAGAA TCGCCAACAA AAACATTCTC AAACGGGCGC 
GTCGTTCTCC GTCAAGCCCG CTTGAAGCTC CACAATCAAA CCCCTAAAGG CTCTGAAGGT 
CCGTTTTCCA GCAATGCAAC AAACGGCTCT CCGCCAGGGA CAGTGACTTG TGGTGTCTGT 
GGTGCCGTCC GTTTCTACCG ATTCGTGAAG CAAGCGCGTA AATTTAACAT CTACAGCTGC 
GAATCATGCC GCAAATTTAT CTCAAAAATG ATAAAACGGC AGTCTTGCGG CAAGAACCTA 
AGCAGTGCCA CTCTGGTCTG CCACAAGGGA CAAGGAATGT GTCACGTACC CCCCATAGTG 
CGCAGCCAAC AAATGAAACT TATACGGTGC GCCTACAAGT CCCGATGCCC TGCCTGTTGG 
CTGAAAATGT GCCTTCGCTC CTTCCAAATG CCCCAATCTT TGAAAAACAG TCTAATCCAA 
CTACTTCCAA TGAACATGCA AGGCTCTGAC GTTATGTTCA ACAACTCGCT GCCCTTGTTA 
TGGCAGGTCA GCGTTCAGAA TATTAACAAA CCTGAACAGA ACAAAACGGT AAATCAGCGA 
CCTGTCAGGT TTAAAAACAC TAAGCCTGTC ACACAACCTA CTCTCGCACC TCCAACGTCT 
GATATTAAGA GGCAAAAAAT TGATTTGAAA GGCCCGCGAG TGAAACACGT TTGTCGCAGT 
GCTTCTATAG TCTTGGGCCA GCCCCTGGCC ATGTTTCCGA GTGAAACCGA GAAAAAGAGA 
ACTGAGGAAT GCCCGAAAAA AGCACAAAAG ACGAGTTCAA CGCATGCTGA AAACGTTGAA 
ACGCCGCCAC AAGTCAAGTC TTTAAAAGAT GTAGAATGCA GCGAAAATGA ATCCGATAAT 
GATAAATCAA TAAAGGTGCC ATTAGTACTA AATGAAAATT TAATTTCTCA AATTAAGAGG 
GACAGGAGCC ATAGAGTGCC TTTTTCATCC GTATCTTCGT CTTCGTCACT TCCGTCTTTC 
CCTTCACTTT CATCGTCATC GTTTACGTCT TTGAGTGAAA GCAAATCCGA CTTTGAGAAG 
AAACCCAATC TACAAGAAGT CCAAAATATG ATCTCAATTA ATTATTGGGA TCATTACGAC 
TTGGATGACG TCAAGCAAAA TGGATTTTGT CTCATTGCCT CTCAACAATT TCCAATGCCT 
GCTATTTGTT TTCTTTGTGG CAGTGCGGGA CGCGAAGCAC TTTTACATTG TTCTCTCTGT 
TGTGAGCCAT ATCACCCCTT TTGTCTTGAA AGAAGTCCTC CATTTAGTAG CACCAGCAAT 
AATAACAGAC AAAATGTGTG GCTCTGTCCA AGATGTACCA CTTGCAACGC TTGTAATCGA 
GCAGACAGGC AAAAAATCCA TTGTCAGAAG TGTTCCAAAG CTTATCATTC GGAATGTCTA 
AAGACAGACT GGACCGGCAA AGGTAAACCC ACGATTTGTT CGAAATGTCT AAGTTGTAAA 
GACTGCGGAG ATTTAAACAC TACAATTTTT TTTGGGAATT CACCATTATG TGTAACCTGC 
TTTAAACTTC GGAGGAAAGG GTCATGTCCT GTTTGTTTGA TACCATTTAG AGAAATCGAT 
ACAAACGAAA AGATGATGGA ATGTACAAAA TGTAAAAAGT GGATTCACGC GGATTGTGAG 
GATTTATCAG AAGAGTATTA TCACATCTTA AGTTATCTCC CCGAGTCTGT TAAATACTTG 
TGTTGTGCGT GTTCAAAACA ATCGCCGCCT TTTTGGCGTA AATCAATAGA GTCCGAACTA 
AAATATAATT TCAATCGAGT GTTACGGTTA CTAAGTAAAA ACAAAACAGC TAAAAATATG 
TTAAAGTTAA GTCTTTTAAA TAATTACGTT ACGTTGACAA GAACTACCGG TAAGAAAAGT 
GATTGCGAGA ATGACGGCGA TGACAATCAG GAGAATTTAG CTACATATTT TAATAGTTTT 
AATAAGAATA GTGACAATGA GGCACCCTTG AAGAGTTTCA GTGATTTTAA AATCGCCCAA 
ACAAGGACAT TAACTGTGTT AGATATTAAA CGTAAAGCGA ACTCGAATGA ATATAGTTCA 
GTGAAAGACT TTCATAAAGA AATGGAAGAA GGGTTAGCTC AGACACAATC GGATGAGTTG 
CTAAATATCT ATCGAATAAT TCTTAAATCA GTATTCCCTT GGTTTGATAT TTCTCAGGAA 
GACTCGAAAT GGAATAATAC GAAACAACGA TTTTTATGTA ATAATAACAT TGATGAAGAC 
ATTTGTGGTC AAATCGAATG CGTGTCAATA GACTCGAGAT ATTGTTCGTT CTGTAAAAGT 
ATAGGTGATG GTTTAAGCCA TCTTGAATCT CGATTACTTT ACTGCGGGCA AAATGAATGG 
GTACACATAA ACTGTGCTTT ATGGTCTTCT GAAGTCTACG AAGAAATCGA TGGTTCCCTC 
CAAAACGTCC ACAGTGCACT TTCAAGAGGT CGTCTAATGC GTTGCTCCTA CTGTAAACAA 
AAAGGAGCAA CGGTTGGTTG TTGTTTCAAG AACTGTTGCG AGATTTATCA CTTCATTTGC 
GCGAGAACTG CGAAATGTCA TTTTATGCAT GACAAGACTG TATTTTGTTG TAGCCACGAA 
GTACCGAAGA CTAGTAGTGT TATAGTCACA CCTCATGAGT TTAATATTCG TAGATCTGTT 
TTTGTGGAAT TGGCTGATTA CAAAAAGGAG CAATATTCAC AAATTGAAAA AGTTAACGTA 
GTGGTTGGGT CACTCTGTGT TACAAATCTT GGAAAAATAA ATCCAGTTAT GTCTGACACT 
GTTGAGGTGA TCATTCCGGT CGGTTATAAT TGTTCTAGGC TCTTTTGGTC AACTGTCGAG 
CCTTGGAAAT TAGTTTCGTA TAAAATATCT ACTTCGATTT TAAATGCTCA TGTTAATAAT 
CACCACGTTG ATAAGAGTTT CACCGTTGAT CATTCCCTTC CAAAGTCAGT GGTTGAACGT 
AAATTGAAGG AAATCCTCCT TTGGCACAAG GATATAGATA AGAAAAAGCT GGATGTTATT 
GAGTTTGACG ATGAGGAGGA ACCTCAAAAC GGGGCAGATC TTTTGTCACC CGAAATCACA 
GATGCAATTT TTGAGGAACT CCCCCACGAG CTGCTTGATG GTATTTCGGT GCAAGACATT 
TTTCCGAAAC TCATGTCTTA CGAGGATTTT ATTAGTATGG ATTCAAAATC GGAAACGACC 
AACTCCGACA ATAACGGTAA TACTGAATTG AAGAAATCAG ACCAGGAAGT TGAAAATGTG 
GAAGAGTTTG ACGATAAGGA GTTTAAATCG AACAAGAACC CACAAAAAAC GTGTAGTCTG 
ACTCTCAGTT GCAAACTGGA TAACAGCTTT TCTCCAGTCA TTAAGAAACG GAAAATAACT 
CCGCGTGAAA ATAACTTATT TTTTCAATTG CTGCAAGTCG ATGGTAACTT TGATGACAGC 
TCGAGTGATT GTGGGTCACC AGTAGGGGTT AATAGTGTAA ACATATGGGA CTCGGATGTG 
TCTGAAGAAC CGGTCACGTG TGAACGTTGC CAATGTACTT ACCGGACACA AGCGAGCTAC 
AAACGACACT TGGACGCATG CGAAGTTATT TGTACAAGCG AAAGCGATTC GGAATTGAAT 
CAAGATCAAG ATGCGAATCT TGAAACAACT CAAATTCACA TCAACGAAAC AACAGTCAAC 
ACCGAACTTC CACAACCTGT TCTCATCACC TCATACGAAT CTTATCAAAA CGAAATTCAT 
ACCTCTGTTT TAAATACTCA AACTTTCGTA GCGTCGAAAT CAAATGCAGA AGTGATCACT 
GTTCCTCAAA ATCCGGAGAT AATTATTCCG CAGACCGAAC AACCAATAAC CGAACCGATT 
GTTAGTAGTG AAGTGTTCAC AGTATCGCAA TCGACTGTGT CAGTAAATGA ACAAACCTCG 
CAACCGTTCT GTCTAAACCC GACTGTCTCT CTTTGTGTTA ATCCACCTCT AACGTTACAA 
CCAAACGCAA CTATTTCGGT TAATCCGACA CAGTCGGTTA ATCCGATTAT AAACCAAACT 
ATCGCTATTA ACCAAGTGAC TCAACCAGCG ACTGTACAAC CGCATCAGTT GGAGTTTCAA 
CAGCCAGGAG TTACAATACA ATCCATGCCG TACTCCACTA ATGTAGCTCC TCTCATTAAT 
GTAACACCCC AGAATCAGAT TACCCTCACC GATAAGTTAT TGAATAATTC AAGTATTATA 
AATTCGATGA TTAATGTACC TGGAAATCGA TGTGTTATAC CGTCGAAAAG CCCAACAAAG 
GTTTTTAAAG GAAGACGATC GATTGCTGCC AAAAGAGGTC ACGAGGAGGA AACCATTGTG 
ATACCAGCAA GTTCGACTCC AGTGATTGTT CAACACTTGC CATCTAGTAA TTTTATTCCG 
ACTTTTGTTG ACACCTTCCA GCAACAATCA GGACAAAATG TACAATACGT TACAACGATT 
GCACCTCAAA TCAACTCAAC GATCAACCCA CAACCGCTGA TGCAGTTACA GGCTGACAGT 
AATTTGATAA GTTTGTTGCC TGGCATTCAA CCGACGATGA TCATTCAACA GCCACGAGTT 
TTGGAAAATC AATTGATTGT GGACGGGAAT GGTTCATTGA GTTGGACACC ACCACAAGTA 
CAACCGGTCT ATTACGGTTT TGAGACGATA GTACAGAACA CTGTAATGCA GTCTCAACAG 
TTTTTACCAA CAACAGTACC AGGAGTCTTA ACTGCTAACA GCAGTTATTC TACAACGACA 
CAAGTATTCC AAACTAGTAA ATTAGAACCA GTATTAGATA TGGGGACGAA TAGCTTTGTC 
CTTCTAAATA CAGGACAATT GGTCAATTCG CAGTCACTCG AAGTACCTCA GAATTCACTA 
ACGAATGTTT TACAACAAAC AGAAATCAAT CAGTGTAAAT ACTCAACACC TTCATTTCAA 
AAACCGAATA GTCAACCAAT TAGTGTCACT AATACAAACA GTATAACACT ACCTACGGCA 
CCCTTTGTCC CAGAACAAGG GATACCAACT AATATTGTTA CGCCAACACC TAAACCGGTA 
CCAACCACTC AGAGTCGGCC AATGAGTCGC GTCCTACCTA TGCAGACGAA AGAACCAAAC 
AAAGTTAAAA CACCAACAGA AAAAGTTGAA GCAATTAGAA AAGAAATTAA AATCCTGGAG 
AAGGCCGTAG AACCAGTAGT CGATTTTGAT AAAAAGAACC CTATTGAGAG TACCGACAAA 
AAACTCATCG AAACTCAATT AGAAAATGAT ATAAATGAAG CCAAGAAAGA CTCTCCTTTG 
AAACTCGTAT TCCAAAAACA GACCCAAGAC GGTTCGTACA AAGTCAGTAA CAACGTCGAG 
GAACCTCTTA TACCTGCAAT GACTAACCAA ATCAGCCCCA AAGTAGCCAG CAACTTCAAA 
TCCACTGAAC CCTCGTATAA ACTAAACAAC AATTTCAACA ATCGCAACTC TCCAGTACAA 
ATAGCACCTT TAAAACCGAT CAAGTCGAAC TTCAACCCCC CAAAACCACT CCACATTCTG 
TCACTGAGTG ACAAACAAAA CCGGCCTGAA AAAATCGAGA AGAGGCTTGC CATCGAACCT 
CCAAAGCACT TCCCGCTCGA GAAAAAGGAG AATGAGAACG TCCCGTCCAT ACTTTACACA 
ATTGAGACAC AGGATGGCTT CCGGTACTCA TCAACGTCCA TTGCGGACTT GTGGACCAAA 
GTTTTCGAAG CTGTGCAAAA CGCACGGGCG GCTCACAACA TGCCGCTTTT GCCCTCAGAC 
TCGACAAATA TTGTAAACAG TTTGCAAATT CTCGGCTTGA AGACTTCCGG ACTTAAGTAT 
CTCATTGAGC AATTGCCAGG TGCCACCAAG TGCTCGAAGT ATAGGCCAAT GTTCAATTTC 
CCATCAAAGT GTATAGAAAT CGAGGACGAG CTTGTCGGGC ATACAAACGG AAGTGCGAGG 
TGTGCGCCAC ACATCAAAAG AAATGAACCC TACGATATGT TCGGGTGGTT AGCGTCGAAA 
CACCGGAAAC CTGAACATAG TCTATTCTGT TCAGATTTAG TGCCAAGAAG GGGCTCTGTA 
AATAATTTAC CAATGGCCAT GCGATTCCGA CAATTGAAAC TTACTAGTAA ATATTCAGTA 
GGAGTATACC GTTCAAAAAT CCACCGACGA GGCTTATTCT GTTTGAGAGA TTTTGAAGCA 
GGAGAAATGG TTATAGAATA TTCAGGAGAA GTTATTAGAT CAGTTTTGAC TGATAAAAGA 
GAAAAGTATT ATAATTCGAA AGGAATTGGT TGTTACATGT TTCGAATTGA CGATAACTTG 
GTTGTTGATG CAACCATGAC CGGAAATGCG GCCAGGTTTA TAAATCATTC TTGTGATCCG 
AATTGTTATT CGAAAGTCGT TGAAATTTTG GGTCACAAGC ACATCATCAT TTTTGCTTTA 
AGAAGAATCA TTTGTGGAGA AGAATTAACA TACGATTACA AATTTCCCAT CGAGGAAGAT 
AAAATTCCAT GCACTTGTGG AACACGACGT TGTAGGAAAT TTCTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008200708.1
CDS	98..7288
Translation	MGRSKFPGKPSKHVNRKRINVLPPTSEGIIESSRSMTVETVAENKQAENDVEESSESVNLNNTINTKVSLRRRLNRKMQSSKALMSNNSSKRGLISKLCNKNSVSCKLSVKSKLNCKASRNRKSVHAYSPIKNFRQCRSGITKESNNLVGKFVLPTRSVHSSRVIKPNKRFINVDLSDTLTLKKRVINKRHCIKQSENPSKTNKSDSASESPTKTFSNGRVVLRQARLKLHNQTPKGSEGPFSSNATNGSPPGTVTCGVCGAVRFYRFVKQARKFNIYSCESCRKFISKMIKRQSCGKNLSSATLVCHKGQGMCHVPPIVRSQQMKLIRCAYKSRCPACWLKMCLRSFQMPQSLKNSLIQLLPMNMQGSDVMFNNSLPLLWQVSVQNINKPEQNKTVNQRPVRFKNTKPVTQPTLAPPTSDIKRQKIDLKGPRVKHVCRSASIVLGQPLAMFPSETEKKRTEECPKKAQKTSSTHAENVETPPQVKSLKDVECSENESDNDKSIKVPLVLNENLISQIKRDRSHRVPFSSVSSSSSLPSFPSLSSSSFTSLSESKSDFEKKPNLQEVQNMISINYWDHYDLDDVKQNGFCLIASQQFPMPAICFLCGSAGREALLHCSLCCEPYHPFCLERSPPFSSTSNNNRQNVWLCPRCTTCNACNRADRQKIHCQKCSKAYHSECLKTDWTGKGKPTICSKCLSCKDCGDLNTTIFFGNSPLCVTCFKLRRKGSCPVCLIPFREIDTNEKMMECTKCKKWIHADCEDLSEEYYHILSYLPESVKYLCCACSKQSPPFWRKSIESELKYNFNRVLRLLSKNKTAKNMLKLSLLNNYVTLTRTTGKKSDCENDGDDNQENLATYFNSFNKNSDNEAPLKSFSDFKIAQTRTLTVLDIKRKANSNEYSSVKDFHKEMEEGLAQTQSDELLNIYRIILKSVFPWFDISQEDSKWNNTKQRFLCNNNIDEDICGQIECVSIDSRYCSFCKSIGDGLSHLESRLLYCGQNEWVHINCALWSSEVYEEIDGSLQNVHSALSRGRLMRCSYCKQKGATVGCCFKNCCEIYHFICARTAKCHFMHDKTVFCCSHEVPKTSSVIVTPHEFNIRRSVFVELADYKKEQYSQIEKVNVVVGSLCVTNLGKINPVMSDTVEVIIPVGYNCSRLFWSTVEPWKLVSYKISTSILNAHVNNHHVDKSFTVDHSLPKSVVERKLKEILLWHKDIDKKKLDVIEFDDEEEPQNGADLLSPEITDAIFEELPHELLDGISVQDIFPKLMSYEDFISMDSKSETTNSDNNGNTELKKSDQEVENVEEFDDKEFKSNKNPQKTCSLTLSCKLDNSFSPVIKKRKITPRENNLFFQLLQVDGNFDDSSSDCGSPVGVNSVNIWDSDVSEEPVTCERCQCTYRTQASYKRHLDACEVICTSESDSELNQDQDANLETTQIHINETTVNTELPQPVLITSYESYQNEIHTSVLNTQTFVASKSNAEVITVPQNPEIIIPQTEQPITEPIVSSEVFTVSQSTVSVNEQTSQPFCLNPTVSLCVNPPLTLQPNATISVNPTQSVNPIINQTIAINQVTQPATVQPHQLEFQQPGVTIQSMPYSTNVAPLINVTPQNQITLTDKLLNNSSIINSMINVPGNRCVIPSKSPTKVFKGRRSIAAKRGHEEETIVIPASSTPVIVQHLPSSNFIPTFVDTFQQQSGQNVQYVTTIAPQINSTINPQPLMQLQADSNLISLLPGIQPTMIIQQPRVLENQLIVDGNGSLSWTPPQVQPVYYGFETIVQNTVMQSQQFLPTTVPGVLTANSSYSTTTQVFQTSKLEPVLDMGTNSFVLLNTGQLVNSQSLEVPQNSLTNVLQQTEINQCKYSTPSFQKPNSQPISVTNTNSITLPTAPFVPEQGIPTNIVTPTPKPVPTTQSRPMSRVLPMQTKEPNKVKTPTEKVEAIRKEIKILEKAVEPVVDFDKKNPIESTDKKLIETQLENDINEAKKDSPLKLVFQKQTQDGSYKVSNNVEEPLIPAMTNQISPKVASNFKSTEPSYKLNNNFNNRNSPVQIAPLKPIKSNFNPPKPLHILSLSDKQNRPEKIEKRLAIEPPKHFPLEKKENENVPSILYTIETQDGFRYSSTSIADLWTKVFEAVQNARAAHNMPLLPSDSTNIVNSLQILGLKTSGLKYLIEQLPGATKCSKYRPMFNFPSKCIEIEDELVGHTNGSARCAPHIKRNEPYDMFGWLASKHRKPEHSLFCSDLVPRRGSVNNLPMAMRFRQLKLTSKYSVGVYRSKIHRRGLFCLRDFEAGEMVIEYSGEVIRSVLTDKREKYYNSKGIGCYMFRIDDNLVVDATMTGNAARFINHSCDPNCYSKVVEILGHKHIIIFALRRIICGEELTYDYKFPIEEDKIPCTCGTRRCRKFLN

Target ORF information:

RefSeq Version	XM_008202486.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum histone-lysine N-methyltransferase trithorax (LOC658843), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008202486.2

ORF Insert Sequence:

ATGGGCCGTT CAAAATTTCC CGGAAAACCA TCGAAACATG TTAATAGGAA AAGAATTAAT 
GTTCTTCCAC CCACCTCGGA GGGAATTATT GAATCTAGCA GGTCTATGAC AGTTGAAACT 
GTTGCAGAAA ACAAACAGGC CGAAAATGAT GTAGAAGAAA GCAGTGAAAG TGTAAATTTA 
AACAATACTA TAAATACAAA AGTGTCATTG AGAAGAAGAC TGAACCGAAA AATGCAAAGT 
TCGAAAGCAT TGATGAGCAA TAATTCGTCT AAACGTGGTC TAATATCAAA ACTCTGTAAT 
AAAAATAGTG TTTCCTGCAA ACTCTCGGTT AAAAGCAAAT TAAATTGCAA AGCATCAAGG 
AATCGCAAAT CAGTGCATGC ATACTCTCCA ATAAAAAATT TCCGCCAGTG TCGAAGTGGA 
ATAACAAAAG AAAGTAATAA TCTCGTTGGA AAATTCGTAT TACCCACAAG AAGTGTCCAT 
TCATCGCGTG TGATAAAACC AAACAAAAGA TTCATAAACG TGGATTTGAG TGACACATTA 
ACCCTAAAAA AGCGCGTCAT TAACAAAAGA CATTGTATAA AACAAAGCGA AAATCCGTCG 
AAAACCAACA AAAGTGACAG TGCATCAGAA TCGCCAACAA AAACATTCTC AAACGGGCGC 
GTCGTTCTCC GTCAAGCCCG CTTGAAGCTC CACAATCAAA CCCCTAAAGG CTCTGAAGGT 
CCGTTTTCCA GCAATGCAAC AAACGGCTCT CCGCCAGGGA CAGTGACTTG TGGTGTCTGT 
GGTGCCGTCC GTTTCTACCG ATTCGTGAAG CAAGCGCGTA AATTTAACAT CTACAGCTGC 
GAATCATGCC GCAAATTTAT CTCAAAAATG ATAAAACGGC AGTCTTGCGG CAAGAACCTA 
AGCAGTGCCA CTCTGGTCTG CCACAAGGGA CAAGGAATGT GTCACGTACC CCCCATAGTG 
CGCAGCCAAC AAATGAAACT TATACGGTGC GCCTACAAGT CCCGATGCCC TGCCTGTTGG 
CTGAAAATGT GCCTTCGCTC CTTCCAAATG CCCCAATCTT TGAAAAACAG TCTAATCCAA 
CTACTTCCAA TGAACATGCA AGGCTCTGAC GTTATGTTCA ACAACTCGCT GCCCTTGTTA 
TGGCAGGTCA GCGTTCAGAA TATTAACAAA CCTGAACAGA ACAAAACGGT AAATCAGCGA 
CCTGTCAGGT TTAAAAACAC TAAGCCTGTC ACACAACCTA CTCTCGCACC TCCAACGTCT 
GATATTAAGA GGCAAAAAAT TGATTTGAAA GGCCCGCGAG TGAAACACGT TTGTCGCAGT 
GCTTCTATAG TCTTGGGCCA GCCCCTGGCC ATGTTTCCGA GTGAAACCGA GAAAAAGAGA 
ACTGAGGAAT GCCCGAAAAA AGCACAAAAG ACGAGTTCAA CGCATGCTGA AAACGTTGAA 
ACGCCGCCAC AAGTCAAGTC TTTAAAAGAT GTAGAATGCA GCGAAAATGA ATCCGATAAT 
GATAAATCAA TAAAGGTGCC ATTAGTACTA AATGAAAATT TAATTTCTCA AATTAAGAGG 
GACAGGAGCC ATAGAGTGCC TTTTTCATCC GTATCTTCGT CTTCGTCACT TCCGTCTTTC 
CCTTCACTTT CATCGTCATC GTTTACGTCT TTGAGTGAAA GCAAATCCGA CTTTGAGAAG 
AAACCCAATC TACAAGAAGT CCAAAATATG ATCTCAATTA ATTATTGGGA TCATTACGAC 
TTGGATGACG TCAAGCAAAA TGGATTTTGT CTCATTGCCT CTCAACAATT TCCAATGCCT 
GCTATTTGTT TTCTTTGTGG CAGTGCGGGA CGCGAAGCAC TTTTACATTG TTCTCTCTGT 
TGTGAGCCAT ATCACCCCTT TTGTCTTGAA AGAAGTCCTC CATTTAGTAG CACCAGCAAT 
AATAACAGAC AAAATGTGTG GCTCTGTCCA AGATGTACCA CTTGCAACGC TTGTAATCGA 
GCAGACAGGC AAAAAATCCA TTGTCAGAAG TGTTCCAAAG CTTATCATTC GGAATGTCTA 
AAGACAGACT GGACCGGCAA AGGTAAACCC ACGATTTGTT CGAAATGTCT AAGTTGTAAA 
GACTGCGGAG ATTTAAACAC TACAATTTTT TTTGGGAATT CACCATTATG TGTAACCTGC 
TTTAAACTTC GGAGGAAAGG GTCATGTCCT GTTTGTTTGA TACCATTTAG AGAAATCGAT 
ACAAACGAAA AGATGATGGA ATGTACAAAA TGTAAAAAGT GGATTCACGC GGATTGTGAG 
GATTTATCAG AAGAGTATTA TCACATCTTA AGTTATCTCC CCGAGTCTGT TAAATACTTG 
TGTTGTGCGT GTTCAAAACA ATCGCCGCCT TTTTGGCGTA AATCAATAGA GTCCGAACTA 
AAATATAATT TCAATCGAGT GTTACGGTTA CTAAGTAAAA ACAAAACAGC TAAAAATATG 
TTAAAGTTAA GTCTTTTAAA TAATTACGTT ACGTTGACAA GAACTACCGG TAAGAAAAGT 
GATTGCGAGA ATGACGGCGA TGACAATCAG GAGAATTTAG CTACATATTT TAATAGTTTT 
AATAAGAATA GTGACAATGA GGCACCCTTG AAGAGTTTCA GTGATTTTAA AATCGCCCAA 
ACAAGGACAT TAACTGTGTT AGATATTAAA CGTAAAGCGA ACTCGAATGA ATATAGTTCA 
GTGAAAGACT TTCATAAAGA AATGGAAGAA GGGTTAGCTC AGACACAATC GGATGAGTTG 
CTAAATATCT ATCGAATAAT TCTTAAATCA GTATTCCCTT GGTTTGATAT TTCTCAGGAA 
GACTCGAAAT GGAATAATAC GAAACAACGA TTTTTATGTA ATAATAACAT TGATGAAGAC 
ATTTGTGGTC AAATCGAATG CGTGTCAATA GACTCGAGAT ATTGTTCGTT CTGTAAAAGT 
ATAGGTGATG GTTTAAGCCA TCTTGAATCT CGATTACTTT ACTGCGGGCA AAATGAATGG 
GTACACATAA ACTGTGCTTT ATGGTCTTCT GAAGTCTACG AAGAAATCGA TGGTTCCCTC 
CAAAACGTCC ACAGTGCACT TTCAAGAGGT CGTCTAATGC GTTGCTCCTA CTGTAAACAA 
AAAGGAGCAA CGGTTGGTTG TTGTTTCAAG AACTGTTGCG AGATTTATCA CTTCATTTGC 
GCGAGAACTG CGAAATGTCA TTTTATGCAT GACAAGACTG TATTTTGTTG TAGCCACGAA 
GTACCGAAGA CTAGTAGTGT TATAGTCACA CCTCATGAGT TTAATATTCG TAGATCTGTT 
TTTGTGGAAT TGGCTGATTA CAAAAAGGAG CAATATTCAC AAATTGAAAA AGTTAACGTA 
GTGGTTGGGT CACTCTGTGT TACAAATCTT GGAAAAATAA ATCCAGTTAT GTCTGACACT 
GTTGAGGTGA TCATTCCGGT CGGTTATAAT TGTTCTAGGC TCTTTTGGTC AACTGTCGAG 
CCTTGGAAAT TAGTTTCGTA TAAAATATCT ACTTCGATTT TAAATGCTCA TGTTAATAAT 
CACCACGTTG ATAAGAGTTT CACCGTTGAT CATTCCCTTC CAAAGTCAGT GGTTGAACGT 
AAATTGAAGG AAATCCTCCT TTGGCACAAG GATATAGATA AGAAAAAGCT GGATGTTATT 
GAGTTTGACG ATGAGGAGGA ACCTCAAAAC GGGGCAGATC TTTTGTCACC CGAAATCACA 
GATGCAATTT TTGAGGAACT CCCCCACGAG CTGCTTGATG GTATTTCGGT GCAAGACATT 
TTTCCGAAAC TCATGTCTTA CGAGGATTTT ATTAGTATGG ATTCAAAATC GGAAACGACC 
AACTCCGACA ATAACGGTAA TACTGAATTG AAGAAATCAG ACCAGGAAGT TGAAAATGTG 
GAAGAGTTTG ACGATAAGGA GTTTAAATCG AACAAGAACC CACAAAAAAC GTGTAGTCTG 
ACTCTCAGTT GCAAACTGGA TAACAGCTTT TCTCCAGTCA TTAAGAAACG GAAAATAACT 
CCGCGTGAAA ATAACTTATT TTTTCAATTG CTGCAAGTCG ATGGTAACTT TGATGACAGC 
TCGAGTGATT GTGGGTCACC AGTAGGGGTT AATAGTGTAA ACATATGGGA CTCGGATGTG 
TCTGAAGAAC CGGTCACGTG TGAACGTTGC CAATGTACTT ACCGGACACA AGCGAGCTAC 
AAACGACACT TGGACGCATG CGAAGTTATT TGTACAAGCG AAAGCGATTC GGAATTGAAT 
CAAGATCAAG ATGCGAATCT TGAAACAACT CAAATTCACA TCAACGAAAC AACAGTCAAC 
ACCGAACTTC CACAACCTGT TCTCATCACC TCATACGAAT CTTATCAAAA CGAAATTCAT 
ACCTCTGTTT TAAATACTCA AACTTTCGTA GCGTCGAAAT CAAATGCAGA AGTGATCACT 
GTTCCTCAAA ATCCGGAGAT AATTATTCCG CAGACCGAAC AACCAATAAC CGAACCGATT 
GTTAGTAGTG AAGTGTTCAC AGTATCGCAA TCGACTGTGT CAGTAAATGA ACAAACCTCG 
CAACCGTTCT GTCTAAACCC GACTGTCTCT CTTTGTGTTA ATCCACCTCT AACGTTACAA 
CCAAACGCAA CTATTTCGGT TAATCCGACA CAGTCGGTTA ATCCGATTAT AAACCAAACT 
ATCGCTATTA ACCAAGTGAC TCAACCAGCG ACTGTACAAC CGCATCAGTT GGAGTTTCAA 
CAGCCAGGAG TTACAATACA ATCCATGCCG TACTCCACTA ATGTAGCTCC TCTCATTAAT 
GTAACACCCC AGAATCAGAT TACCCTCACC GATAAGTTAT TGAATAATTC AAGTATTATA 
AATTCGATGA TTAATGTACC TGGAAATCGA TGTGTTATAC CGTCGAAAAG CCCAACAAAG 
GTTTTTAAAG GAAGACGATC GATTGCTGCC AAAAGAGGTC ACGAGGAGGA AACCATTGTG 
ATACCAGCAA GTTCGACTCC AGTGATTGTT CAACACTTGC CATCTAGTAA TTTTATTCCG 
ACTTTTGTTG ACACCTTCCA GCAACAATCA GGACAAAATG TACAATACGT TACAACGATT 
GCACCTCAAA TCAACTCAAC GATCAACCCA CAACCGCTGA TGCAGTTACA GGCTGACAGT 
AATTTGATAA GTTTGTTGCC TGGCATTCAA CCGACGATGA TCATTCAACA GCCACGAGTT 
TTGGAAAATC AATTGATTGT GGACGGGAAT GGTTCATTGA GTTGGACACC ACCACAAGTA 
CAACCGGTCT ATTACGGTTT TGAGACGATA GTACAGAACA CTGTAATGCA GTCTCAACAG 
TTTTTACCAA CAACAGTACC AGGAGTCTTA ACTGCTAACA GCAGTTATTC TACAACGACA 
CAAGTATTCC AAACTAGTAA ATTAGAACCA GTATTAGATA TGGGGACGAA TAGCTTTGTC 
CTTCTAAATA CAGGACAATT GGTCAATTCG CAGTCACTCG AAGTACCTCA GAATTCACTA 
ACGAATGTTT TACAACAAAC AGAAATCAAT CAGTGTAAAT ACTCAACACC TTCATTTCAA 
AAACCGAATA GTCAACCAAT TAGTGTCACT AATACAAACA GTATAACACT ACCTACGGCA 
CCCTTTGTCC CAGAACAAGG GATACCAACT AATATTGTTA CGCCAACACC TAAACCGGTA 
CCAACCACTC AGAGTCGGCC AATGAGTCGC GTCCTACCTA TGCAGACGAA AGAACCAAAC 
AAAGTTAAAA CACCAACAGA AAAAGTTGAA GCAATTAGAA AAGAAATTAA AATCCTGGAG 
AAGGCCGTAG AACCAGTAGT CGATTTTGAT AAAAAGAACC CTATTGAGAG TACCGACAAA 
AAACTCATCG AAACTCAATT AGAAAATGAT ATAAATGAAG CCAAGAAAGA CTCTCCTTTG 
AAACTCGTAT TCCAAAAACA GACCCAAGAC GGTTCGTACA AAGTCAGTAA CAACGTCGAG 
GAACCTCTTA TACCTGCAAT GACTAACCAA ATCAGCCCCA AAGTAGCCAG CAACTTCAAA 
TCCACTGAAC CCTCGTATAA ACTAAACAAC AATTTCAACA ATCGCAACTC TCCAGTACAA 
ATAGCACCTT TAAAACCGAT CAAGTCGAAC TTCAACCCCC CAAAACCACT CCACATTCTG 
TCACTGAGTG ACAAACAAAA CCGGCCTGAA AAAATCGAGA AGAGGCTTGC CATCGAACCT 
CCAAAGCACT TCCCGCTCGA GAAAAAGGAG AATGAGAACG TCCCGTCCAT ACTTTACACA 
ATTGAGACAC AGGATGGCTT CCGGTACTCA TCAACGTCCA TTGCGGACTT GTGGACCAAA 
GTTTTCGAAG CTGTGCAAAA CGCACGGGCG GCTCACAACA TGCCGCTTTT GCCCTCAGAC 
TCGACAAATA TTGTAAACAG TTTGCAAATT CTCGGCTTGA AGACTTCCGG ACTTAAGTAT 
CTCATTGAGC AATTGCCAGG TGCCACCAAG TGCTCGAAGT ATAGGCCAAT GTTCAATTTC 
CCATCAAAGT GTATAGAAAT CGAGGACGAG CTTGTCGGGC ATACAAACGG AAGTGCGAGG 
TGTGCGCCAC ACATCAAAAG AAATGAACCC TACGATATGT TCGGGTGGTT AGCGTCGAAA 
CACCGGAAAC CTGAACATAG TCTATTCTGT TCAGATTTAG TGCCAAGAAG GGGCTCTGTA 
AATAATTTAC CAATGGCCAT GCGATTCCGA CAATTGAAAC TTACTAGTAA ATATTCAGTA 
GGAGTATACC GTTCAAAAAT CCACCGACGA GGCTTATTCT GTTTGAGAGA TTTTGAAGCA 
GGAGAAATGG TTATAGAATA TTCAGGAGAA GTTATTAGAT CAGTTTTGAC TGATAAAAGA 
GAAAAGTATT ATAATTCGAA AGGAATTGGT TGTTACATGT TTCGAATTGA CGATAACTTG 
GTTGTTGATG CAACCATGAC CGGAAATGCG GCCAGGTTTA TAAATCATTC TTGTGATCCG 
AATTGTTATT CGAAAGTCGT TGAAATTTTG GGTCACAAGC ACATCATCAT TTTTGCTTTA 
AGAAGAATCA TTTGTGGAGA AGAATTAACA TACGATTACA AATTTCCCAT CGAGGAAGAT 
AAAATTCCAT GCACTTGTGG AACACGACGT TGTAGGAAAT TTCTCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC658843 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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