Dcr-2 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dcr-2
Entrez Gene ID	658023
Full Name	Dicer-2
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008203274.2	XP_008201496.1	dicer-2 isoform X1
XM_015984027.1	XP_015839513.1	dicer-2 isoform X1
NM_001114368.1	NP_001107840.1	Dicer-2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium.
Tomoyasu Y, Miller SC, Tomita S, Schoppmeier M, Grossmann D, Bucher G
Genome biology9(1)R10(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dcr-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dcr-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTg01906	NM_001114368.1 Latest version!	Tribolium castaneum Dicer-2 (Dcr-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTg01906	XM_008203274.2 Latest version!	Tribolium castaneum Dicer-2 (Dcr-2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTg01906	XM_015984027.1 Latest version!	Tribolium castaneum Dicer-2 (Dcr-2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dcr-2 ( NM_001114368.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> NP_001107840.1 Tribolium castaneum Dicer-2 (Dcr-2), mRNA.

Price & Availability↑

CloneID	OTg01906
Clone ID Related Accession (Same CDS sequence)	XM_008203274.2 , XM_015984027.1 , NM_001114368.1
Accession Version	NM_001114368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1536595200000
Organism	Tribolium castaneum(red flour beetle)
Product	Dicer-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAJJ02000460.1. On Feb 7, 2008 this sequence version replaced XM_964437.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1523770, SAMEA1523771 [ECO:0000348] ##Evidence-Data-END##

ATGGACGAAG AAGACGAACT TAAACCTCGT AACTACCAAG TCAATTTGAT GGAAATTGCC 
ATTCGGGAAA ACACAATTAT TTATCTACCG ACTGGATCGG GTAAAACTTT TATTGCAATA 
ATGGTTTTGA AGCAGTTGTG CGCCCCCATA TTGAGACCCT ACAGCGATGG TGGAAAGATT 
TCAGTTATAT TGGTTAATTC TGTGGCTTTG GTGGATCAAC ATGGTAAGTA TGTAAGAGAC 
CACGCCACGT TCAGTGTGGG AACTTACACT GGAGAGATGA ATGTGGATTT TTGGAGCGAG 
GCAGAATGGG AACAGCAATT TAATAAGTAT CAAGTTGTTA TAATGACCAG CCAAATCATG 
GTAAATCTCA TCAACAACCG CTTCATAGAT TTGGGAAAAG TAAACTTGAT GATTTTCGAT 
GAATGCCACC ATGGTGTGGA AGACCAGCCA ATGCGCCAGA TAATGAAACA CTTCCACAGC 
TGTACCGACA AGCCTCGAGT TCTTGGTCTT ACAGCCACGT TATTAAATGG CAATTGCAAA 
TTGTCGAAAG TCATGGATGA AATAAGGTCT TTGGAGGTGA CCTTCCACAG TAAAGTGGCC 
ACTGTGGAAG GATTAGACGT AGTTGTTGGA TATTCGACCA ATCCGCAAGA ACTATTTAAA 
GTTTGCCAGC CGGGCGCTTT GTCGCTTGAT GCCAAACAAG TACTGAACAA CTTGCGTCAA 
CTGATAAATG ATTTGGAACA TATTAATATC AAAGATGAGC AAAACAGTGT GAATTTATTA 
CAGTCTGAAA CCTTGAAACC GCTTGAGCCA TCCGATGTGT TGAAAAGTCT GAGAAACTTG 
ATTTCAGACC TAATGATTCA CATTGAAATG TTAGGGGCAT TCGGTGGTCA CATTGCCTGT 
GTGGCGCATA TGATCCAAAT AGAACGTATA AAAAAGCACT GCCAGAACCA CCAACTCTTC 
ATAGTGTTGA ATTACGTCAT GACCATAATG GGGACGACAA AACTACTTCT GGAGGAAACC 
ATGGCCGGTT ATGAGCCGCT CGAAAAAATT CGAAAATTCT CCTCTGACAA AGTACTGAAA 
GTGTTTGAAA TTTTGGACGA ATACAAAACC AAATCGGACG AGGAACTTTG CTGTTTAGTC 
TTCACCAAAA GGCGGTTCAC AGCCAAGGTC CTCCACCACA TTATCGACAA AGCTAGCCAA 
GTTGATCCCA AATTCTATCA TATCAAGTCA AATTTCGTCG TTGGAAATAA GAATAATCCC 
TACAACGACA CCCGAGAGAA TTTATATATA ACAAAAAAGA ACCGAGAGGT GCTCAACAGC 
TTTGTCAGCA AGGAAATCAA CGTTTTGGTC AGCTCGAACG TGTTGGAGGA GGGAGTGGAC 
ATACCTAAGT GCACTCTCGT CATCAAATTC GACAAATCGG AGGACTACCG TTCGTATATC 
CAGTCGAAAG GACGCGCAAG ACATATCAAA AGTTTATACT ACACCATTGT AGAAACGACT 
GACGTAGCAA AGTACGATAA AAAATACTCC GCTTTCAAGG AAATCGAAAA CCTGGTGAAT 
GATCTTCTCA TCGGCAAAAA TTCTGAAAGA GATCATCCAA ACTTGTCGGA AATCCGCAAC 
ATGTATAATG AAGACAAGCT TGAGCCCTAC TACGTAAACG GCCCCAATTC GGCACAAGTA 
AACATGACCT CGGCCGTAGC TCTCTTGTGC CGTTACTGCA GTAATTTAGC AAGTGACAAA 
TACACCACTT ACGCCCCTGA GTGGTATTAT GAAGAGGATT CTAGCAGCGC AAAATTGCGG 
GTTGTGATAT TTTTGCCCGT TGTGTGCCCT CTTATCGACC CCATTGTCGG CCCTTACATG 
CACAACAAAA AAGACGCAAA AAGAGCAGCC GCACTAGTCG CATGTATAAA GCTACACCAG 
TGCGGCGAAC TTGACAATAA TTTGTTGCCA TGGAAGAAGC AGTTGGATGA AGCCGATGTT 
TCCTATCTTT TCACGCACTG GCCACAAGAA AAGGAAAGCG ACGCCGGAAA TAAGAAAAAG 
AAGAGGTTGC ACGATAAAGA GATCGCTCCG AGTGTGAAGA GTGCAATCCA GCCGGACAGA 
GTCCTCTATT TGCACACTAT TAACATAAAT CCGCAATACA AAAGAAGCGA CGATTTGAAA 
AACGCGGTCA CTATTTACGA CCTGTACAAA ACACCGTTAA AGTTTGGTTT GTTATCGCCG 
AAACCGTTGC CCGATTTGTG CAAATTTCCC CTCTTTGATT CAAATGGAAC GTTGGAAATT 
GAAATTAGGA ACAATGTTAG AGAGGTGGAG TTTGCAGCTA ACGAGATGAA GGAAATGAGG 
GAGTTCCATT TTTTGGTATT CAACGACCTT TTGGAGATTC TGAAAGAGTT TTTAATTTTC 
GACAACACTG GGATGAATTC GGAGATGTTG CTTGTTGTAC CGGTTCAGGA TCGGTGTGGG 
GATGTTTGCG TCGATTTTCG AGTGATTCGA GACAATAAAA ATTTGAAAAA TAAGCTCGAA 
CCGGCGGCTA CGGAACGCAT AAATTTGAAC GTAACTGAGG AAACTTACTT ACATAAGATT 
GTCTCTCCGT GGTATAGATC GCCACCAAAG ATGTACGTTG TGACGAAGGT TTGTCCTGAT 
AAGAGCGCCC TGAGCCGGTT TCCTAACCAC GAATATCCCA ATTTCGTGTC GTATTACTCC 
GAAAAACACT CTTTGTCGAT ATTGGACCCT TCGCAACCGC TGCTCTTGGT TAAAGGGCTT 
TCCGAACGGT TGAACGCTTT CAAACCGAGA GGTGCCGGTG GCAAGAGAAA GAAGGAAAAG 
ATGTATGAAG AATTGGAAGA ATATCTAATA CCAGAGCTGG TGATCAAGCA AGAATTTCCG 
TCGTGTTTGT GGATACAAGC GCGATTTTTG CCAAGCATTT TATCCCGCTT GGCTTACCTT 
CTAAAACTGC AACAACTCCA AGTTGACATA GCTCGTGGTA TTGGGGCCAA AGCCGAATAT 
TTGAAAGACT GTCCGCCGCT GGAGTTAAAC CTCCACTTAT TGCATTATGA GCCCAATGAC 
CCGCAACTAA CGCAAGAATC AGACAAGTCC ACTCCTTTGA TTGACAATTG CCTTGCGTTG 
GAGTGCCCGA AAAATTTGCG AACGATTCAA TACAATAAAG ATTTTGCGGC GAAAATGCTG 
GAAGCGGAGT ACTACTGGAA GACTATTGAA GAACCCAAAG ACATAGAGCG CAATATTAAC 
GTGACTGTTA TGGATATAGA GTATTACGAG ACTTTTATTT CGCATCAGCC TTCCAAAACC 
GGGCGTTTGT TGAAGAATGA CAGCCCGGTT AAGCAACAGA ATGTGCCCGC TATTACGTAC 
GATTGCCAAT TTGAAGCGAA ACAATTGCAA ATTCTGGACG TACAATTCGA CAATCAGAGC 
CCGAACTTGT GCCAGATTTA TCAAGCCTTG ACGGCAGCTG AAGCCAACGA TATCGTCAAC 
CTCGAACGGC TTGAAACCCT CGGCGACTCT TTCTTAAAAT TCGTAGCTTC CTTGTACATT 
ATTTTCAAAT TTCCGACCTA CAACGAGGGC AAATCGACCA CTCTCAAAGG GAAGCTGGTC 
AGCAACAAGA ATTTGTACTA CTTGGGAGTG AGGAAAAATC TGGGCGGTAT TTTGAAAAAC 
AGCGACTTGT CCCCGAGCGA TTGGGTCCCT CCGTGCTTTT GCATCCCACA AACAATCAGC 
AAAGCAATCG GCAATAAAGA GTATTCAGTA GTCTCGTTAT TTAACTGTTG CATTTCGCCG 
GAGGAGCAAG TTTCAGGTAA CTTGAACCGA AAGACTTTGA GCGATATGAC AACGGAAGAA 
ATTGCCCCGG ATGAGGAAAA TTCGTACGGC AATATGTGCA ACTTTTTGAA CAAACAATAC 
GTTGGTGATA AGAGCATAGC GGACTCTGTT GAGGCCCTCC TAGGCGCCTA CTTTCTAAGT 
GGCGGGATTC AAGGCGGGAT CAAATTCATG GAATGGATCG GCATTTTGCC CCTTTCTGAA 
CAAATCCAAC GCCTGATCGA AACCACTCAA GTCGACCCCG TCCTCAATAA AAAATCCACG 
AAAACTGATG TCGATTTCCA CATGCCACAG TGGCGCGAAA TCGAGCAACG TCTCGGTTAT 
ACGTTTACCA ACCGGGCGTT TTTACTCCAA GCTTTAACTC ATTCTTCCTA TTCGCCGAAT 
CGCATAACAC TGAGCTACGA ACGGTTGGAG TTTCTTGGTG ATGCTGTGCT TGATTTCCTC 
ATCACGTGTT ATATTTTCGA ACATTGTGGG CATTTGGAGC CGGGTCAAGT GACCGATTTG 
CGGTCATCCC TTGTCAACAA TAACACGTTC GCAAGCCTTG TTGTCCGATG CGGGTTTCAC 
AAGTTTTTGC TCATGATGAA TTCGAATTTG CAAGGTCATA TTGACAAGTT TGCCGATTAC 
CTTGCTTCAA AAAATTACGT GATCGACGAT GAAGTTCTGA TTTTGTTGGA GGAGGATGAG 
ATGAATATCG CCGAGTACGT CGATGTGCCT AAAGTTTTGG GTGATATTTT CGAAGCCCTT 
GCTGGCGCAA TTTATCTAGA TAGCAATAAG GATTTGAAGA CTGTTTGGAG GGTGTTTTAC 
AAGATTATCT GGCGCGAAAT TGATCTTTTT AGCAAGAATG TGCCAAAAAA TGTCATCAGG 
AGATTGTACG AGTGCCATAC TGTTTACCCT CCGCAATTTA GTAAAGCGTT GGAAGTTGGT 
AATCAAAAGA CTATGGTTTC TTTAGACTTT ATGTGTGAAG GTAGAAAGAA ACGAGTTCAT 
GGGTTTGGAA CGAACAAAAT CTTGGCTAAA AGGGCCGCTG CCAAAATTGC TCTTCGTGCT 
TTAAAGTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107840.1
CDS	1..4872
Translation	MDEEDELKPRNYQVNLMEIAIRENTIIYLPTGSGKTFIAIMVLKQLCAPILRPYSDGGKISVILVNSVALVDQHGKYVRDHATFSVGTYTGEMNVDFWSEAEWEQQFNKYQVVIMTSQIMVNLINNRFIDLGKVNLMIFDECHHGVEDQPMRQIMKHFHSCTDKPRVLGLTATLLNGNCKLSKVMDEIRSLEVTFHSKVATVEGLDVVVGYSTNPQELFKVCQPGALSLDAKQVLNNLRQLINDLEHINIKDEQNSVNLLQSETLKPLEPSDVLKSLRNLISDLMIHIEMLGAFGGHIACVAHMIQIERIKKHCQNHQLFIVLNYVMTIMGTTKLLLEETMAGYEPLEKIRKFSSDKVLKVFEILDEYKTKSDEELCCLVFTKRRFTAKVLHHIIDKASQVDPKFYHIKSNFVVGNKNNPYNDTRENLYITKKNREVLNSFVSKEINVLVSSNVLEEGVDIPKCTLVIKFDKSEDYRSYIQSKGRARHIKSLYYTIVETTDVAKYDKKYSAFKEIENLVNDLLIGKNSERDHPNLSEIRNMYNEDKLEPYYVNGPNSAQVNMTSAVALLCRYCSNLASDKYTTYAPEWYYEEDSSSAKLRVVIFLPVVCPLIDPIVGPYMHNKKDAKRAAALVACIKLHQCGELDNNLLPWKKQLDEADVSYLFTHWPQEKESDAGNKKKKRLHDKEIAPSVKSAIQPDRVLYLHTININPQYKRSDDLKNAVTIYDLYKTPLKFGLLSPKPLPDLCKFPLFDSNGTLEIEIRNNVREVEFAANEMKEMREFHFLVFNDLLEILKEFLIFDNTGMNSEMLLVVPVQDRCGDVCVDFRVIRDNKNLKNKLEPAATERINLNVTEETYLHKIVSPWYRSPPKMYVVTKVCPDKSALSRFPNHEYPNFVSYYSEKHSLSILDPSQPLLLVKGLSERLNAFKPRGAGGKRKKEKMYEELEEYLIPELVIKQEFPSCLWIQARFLPSILSRLAYLLKLQQLQVDIARGIGAKAEYLKDCPPLELNLHLLHYEPNDPQLTQESDKSTPLIDNCLALECPKNLRTIQYNKDFAAKMLEAEYYWKTIEEPKDIERNINVTVMDIEYYETFISHQPSKTGRLLKNDSPVKQQNVPAITYDCQFEAKQLQILDVQFDNQSPNLCQIYQALTAAEANDIVNLERLETLGDSFLKFVASLYIIFKFPTYNEGKSTTLKGKLVSNKNLYYLGVRKNLGGILKNSDLSPSDWVPPCFCIPQTISKAIGNKEYSVVSLFNCCISPEEQVSGNLNRKTLSDMTTEEIAPDEENSYGNMCNFLNKQYVGDKSIADSVEALLGAYFLSGGIQGGIKFMEWIGILPLSEQIQRLIETTQVDPVLNKKSTKTDVDFHMPQWREIEQRLGYTFTNRAFLLQALTHSSYSPNRITLSYERLEFLGDAVLDFLITCYIFEHCGHLEPGQVTDLRSSLVNNNTFASLVVRCGFHKFLLMMNSNLQGHIDKFADYLASKNYVIDDEVLILLEEDEMNIAEYVDVPKVLGDIFEALAGAIYLDSNKDLKTVWRVFYKIIWREIDLFSKNVPKNVIRRLYECHTVYPPQFSKALEVGNQKTMVSLDFMCEGRKKRVHGFGTNKILAKRAAAKIALRALKL

Target ORF information:

RefSeq Version	NM_001114368.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum Dicer-2 (Dcr-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114368.1

ORF Insert Sequence:

ATGGACGAAG AAGACGAACT TAAACCTCGT AACTACCAAG TCAATTTGAT GGAAATTGCC 
ATTCGGGAAA ACACAATTAT TTATCTACCG ACTGGATCGG GTAAAACTTT TATTGCAATA 
ATGGTTTTGA AGCAGTTGTG CGCCCCCATA TTGAGACCCT ACAGCGATGG TGGAAAGATT 
TCAGTTATAT TGGTTAATTC TGTGGCTTTG GTGGATCAAC ATGGTAAGTA TGTAAGAGAC 
CACGCCACGT TCAGTGTGGG AACTTACACT GGAGAGATGA ATGTGGATTT TTGGAGCGAG 
GCAGAATGGG AACAGCAATT TAATAAGTAT CAAGTTGTTA TAATGACCAG CCAAATCATG 
GTAAATCTCA TCAACAACCG CTTCATAGAT TTGGGAAAAG TAAACTTGAT GATTTTCGAT 
GAATGCCACC ATGGTGTGGA AGACCAGCCA ATGCGCCAGA TAATGAAACA CTTCCACAGC 
TGTACCGACA AGCCTCGAGT TCTTGGTCTT ACAGCCACGT TATTAAATGG CAATTGCAAA 
TTGTCGAAAG TCATGGATGA AATAAGGTCT TTGGAGGTGA CCTTCCACAG TAAAGTGGCC 
ACTGTGGAAG GATTAGACGT AGTTGTTGGA TATTCGACCA ATCCGCAAGA ACTATTTAAA 
GTTTGCCAGC CGGGCGCTTT GTCGCTTGAT GCCAAACAAG TACTGAACAA CTTGCGTCAA 
CTGATAAATG ATTTGGAACA TATTAATATC AAAGATGAGC AAAACAGTGT GAATTTATTA 
CAGTCTGAAA CCTTGAAACC GCTTGAGCCA TCCGATGTGT TGAAAAGTCT GAGAAACTTG 
ATTTCAGACC TAATGATTCA CATTGAAATG TTAGGGGCAT TCGGTGGTCA CATTGCCTGT 
GTGGCGCATA TGATCCAAAT AGAACGTATA AAAAAGCACT GCCAGAACCA CCAACTCTTC 
ATAGTGTTGA ATTACGTCAT GACCATAATG GGGACGACAA AACTACTTCT GGAGGAAACC 
ATGGCCGGTT ATGAGCCGCT CGAAAAAATT CGAAAATTCT CCTCTGACAA AGTACTGAAA 
GTGTTTGAAA TTTTGGACGA ATACAAAACC AAATCGGACG AGGAACTTTG CTGTTTAGTC 
TTCACCAAAA GGCGGTTCAC AGCCAAGGTC CTCCACCACA TTATCGACAA AGCTAGCCAA 
GTTGATCCCA AATTCTATCA TATCAAGTCA AATTTCGTCG TTGGAAATAA GAATAATCCC 
TACAACGACA CCCGAGAGAA TTTATATATA ACAAAAAAGA ACCGAGAGGT GCTCAACAGC 
TTTGTCAGCA AGGAAATCAA CGTTTTGGTC AGCTCGAACG TGTTGGAGGA GGGAGTGGAC 
ATACCTAAGT GCACTCTCGT CATCAAATTC GACAAATCGG AGGACTACCG TTCGTATATC 
CAGTCGAAAG GACGCGCAAG ACATATCAAA AGTTTATACT ACACCATTGT AGAAACGACT 
GACGTAGCAA AGTACGATAA AAAATACTCC GCTTTCAAGG AAATCGAAAA CCTGGTGAAT 
GATCTTCTCA TCGGCAAAAA TTCTGAAAGA GATCATCCAA ACTTGTCGGA AATCCGCAAC 
ATGTATAATG AAGACAAGCT TGAGCCCTAC TACGTAAACG GCCCCAATTC GGCACAAGTA 
AACATGACCT CGGCCGTAGC TCTCTTGTGC CGTTACTGCA GTAATTTAGC AAGTGACAAA 
TACACCACTT ACGCCCCTGA GTGGTATTAT GAAGAGGATT CTAGCAGCGC AAAATTGCGG 
GTTGTGATAT TTTTGCCCGT TGTGTGCCCT CTTATCGACC CCATTGTCGG CCCTTACATG 
CACAACAAAA AAGACGCAAA AAGAGCAGCC GCACTAGTCG CATGTATAAA GCTACACCAG 
TGCGGCGAAC TTGACAATAA TTTGTTGCCA TGGAAGAAGC AGTTGGATGA AGCCGATGTT 
TCCTATCTTT TCACGCACTG GCCACAAGAA AAGGAAAGCG ACGCCGGAAA TAAGAAAAAG 
AAGAGGTTGC ACGATAAAGA GATCGCTCCG AGTGTGAAGA GTGCAATCCA GCCGGACAGA 
GTCCTCTATT TGCACACTAT TAACATAAAT CCGCAATACA AAAGAAGCGA CGATTTGAAA 
AACGCGGTCA CTATTTACGA CCTGTACAAA ACACCGTTAA AGTTTGGTTT GTTATCGCCG 
AAACCGTTGC CCGATTTGTG CAAATTTCCC CTCTTTGATT CAAATGGAAC GTTGGAAATT 
GAAATTAGGA ACAATGTTAG AGAGGTGGAG TTTGCAGCTA ACGAGATGAA GGAAATGAGG 
GAGTTCCATT TTTTGGTATT CAACGACCTT TTGGAGATTC TGAAAGAGTT TTTAATTTTC 
GACAACACTG GGATGAATTC GGAGATGTTG CTTGTTGTAC CGGTTCAGGA TCGGTGTGGG 
GATGTTTGCG TCGATTTTCG AGTGATTCGA GACAATAAAA ATTTGAAAAA TAAGCTCGAA 
CCGGCGGCTA CGGAACGCAT AAATTTGAAC GTAACTGAGG AAACTTACTT ACATAAGATT 
GTCTCTCCGT GGTATAGATC GCCACCAAAG ATGTACGTTG TGACGAAGGT TTGTCCTGAT 
AAGAGCGCCC TGAGCCGGTT TCCTAACCAC GAATATCCCA ATTTCGTGTC GTATTACTCC 
GAAAAACACT CTTTGTCGAT ATTGGACCCT TCGCAACCGC TGCTCTTGGT TAAAGGGCTT 
TCCGAACGGT TGAACGCTTT CAAACCGAGA GGTGCCGGTG GCAAGAGAAA GAAGGAAAAG 
ATGTATGAAG AATTGGAAGA ATATCTAATA CCAGAGCTGG TGATCAAGCA AGAATTTCCG 
TCGTGTTTGT GGATACAAGC GCGATTTTTG CCAAGCATTT TATCCCGCTT GGCTTACCTT 
CTAAAACTGC AACAACTCCA AGTTGACATA GCTCGTGGTA TTGGGGCCAA AGCCGAATAT 
TTGAAAGACT GTCCGCCGCT GGAGTTAAAC CTCCACTTAT TGCATTATGA GCCCAATGAC 
CCGCAACTAA CGCAAGAATC AGACAAGTCC ACTCCTTTGA TTGACAATTG CCTTGCGTTG 
GAGTGCCCGA AAAATTTGCG AACGATTCAA TACAATAAAG ATTTTGCGGC GAAAATGCTG 
GAAGCGGAGT ACTACTGGAA GACTATTGAA GAACCCAAAG ACATAGAGCG CAATATTAAC 
GTGACTGTTA TGGATATAGA GTATTACGAG ACTTTTATTT CGCATCAGCC TTCCAAAACC 
GGGCGTTTGT TGAAGAATGA CAGCCCGGTT AAGCAACAGA ATGTGCCCGC TATTACGTAC 
GATTGCCAAT TTGAAGCGAA ACAATTGCAA ATTCTGGACG TACAATTCGA CAATCAGAGC 
CCGAACTTGT GCCAGATTTA TCAAGCCTTG ACGGCAGCTG AAGCCAACGA TATCGTCAAC 
CTCGAACGGC TTGAAACCCT CGGCGACTCT TTCTTAAAAT TCGTAGCTTC CTTGTACATT 
ATTTTCAAAT TTCCGACCTA CAACGAGGGC AAATCGACCA CTCTCAAAGG GAAGCTGGTC 
AGCAACAAGA ATTTGTACTA CTTGGGAGTG AGGAAAAATC TGGGCGGTAT TTTGAAAAAC 
AGCGACTTGT CCCCGAGCGA TTGGGTCCCT CCGTGCTTTT GCATCCCACA AACAATCAGC 
AAAGCAATCG GCAATAAAGA GTATTCAGTA GTCTCGTTAT TTAACTGTTG CATTTCGCCG 
GAGGAGCAAG TTTCAGGTAA CTTGAACCGA AAGACTTTGA GCGATATGAC AACGGAAGAA 
ATTGCCCCGG ATGAGGAAAA TTCGTACGGC AATATGTGCA ACTTTTTGAA CAAACAATAC 
GTTGGTGATA AGAGCATAGC GGACTCTGTT GAGGCCCTCC TAGGCGCCTA CTTTCTAAGT 
GGCGGGATTC AAGGCGGGAT CAAATTCATG GAATGGATCG GCATTTTGCC CCTTTCTGAA 
CAAATCCAAC GCCTGATCGA AACCACTCAA GTCGACCCCG TCCTCAATAA AAAATCCACG 
AAAACTGATG TCGATTTCCA CATGCCACAG TGGCGCGAAA TCGAGCAACG TCTCGGTTAT 
ACGTTTACCA ACCGGGCGTT TTTACTCCAA GCTTTAACTC ATTCTTCCTA TTCGCCGAAT 
CGCATAACAC TGAGCTACGA ACGGTTGGAG TTTCTTGGTG ATGCTGTGCT TGATTTCCTC 
ATCACGTGTT ATATTTTCGA ACATTGTGGG CATTTGGAGC CGGGTCAAGT GACCGATTTG 
CGGTCATCCC TTGTCAACAA TAACACGTTC GCAAGCCTTG TTGTCCGATG CGGGTTTCAC 
AAGTTTTTGC TCATGATGAA TTCGAATTTG CAAGGTCATA TTGACAAGTT TGCCGATTAC 
CTTGCTTCAA AAAATTACGT GATCGACGAT GAAGTTCTGA TTTTGTTGGA GGAGGATGAG 
ATGAATATCG CCGAGTACGT CGATGTGCCT AAAGTTTTGG GTGATATTTT CGAAGCCCTT 
GCTGGCGCAA TTTATCTAGA TAGCAATAAG GATTTGAAGA CTGTTTGGAG GGTGTTTTAC 
AAGATTATCT GGCGCGAAAT TGATCTTTTT AGCAAGAATG TGCCAAAAAA TGTCATCAGG 
AGATTGTACG AGTGCCATAC TGTTTACCCT CCGCAATTTA GTAAAGCGTT GGAAGTTGGT 
AATCAAAAGA CTATGGTTTC TTTAGACTTT ATGTGTGAAG GTAGAAAGAA ACGAGTTCAT 
GGGTTTGGAA CGAACAAAAT CTTGGCTAAA AGGGCCGCTG CCAAAATTGC TCTTCGTGCT 
TTAAAGTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcr-2 ( XM_008203274.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008201496.1 Tribolium castaneum Dicer-2 (Dcr-2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTg01906
Clone ID Related Accession (Same CDS sequence)	XM_008203274.2 , XM_015984027.1 , NM_001114368.1
Accession Version	XM_008203274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	dicer-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450135.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008203274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAAG AAGACGAACT TAAACCTCGT AACTACCAAG TCAATTTGAT GGAAATTGCC 
ATTCGGGAAA ACACAATTAT TTATCTACCG ACTGGATCGG GTAAAACTTT TATTGCAATA 
ATGGTTTTGA AGCAGTTGTG CGCCCCCATA TTGAGACCCT ACAGCGATGG TGGAAAGATT 
TCAGTTATAT TGGTTAATTC TGTGGCTTTG GTGGATCAAC ATGGTAAGTA TGTAAGAGAC 
CACGCCACGT TCAGTGTGGG AACTTACACT GGAGAGATGA ATGTGGATTT TTGGAGCGAG 
GCAGAATGGG AACAGCAATT TAATAAGTAT CAAGTTGTTA TAATGACCAG CCAAATCATG 
GTAAATCTCA TCAACAACCG CTTCATAGAT TTGGGAAAAG TAAACTTGAT GATTTTCGAT 
GAATGCCACC ATGGTGTGGA AGACCAGCCA ATGCGCCAGA TAATGAAACA CTTCCACAGC 
TGTACCGACA AGCCTCGAGT TCTTGGTCTT ACAGCCACGT TATTAAATGG CAATTGCAAA 
TTGTCGAAAG TCATGGATGA AATAAGGTCT TTGGAGGTGA CCTTCCACAG TAAAGTGGCC 
ACTGTGGAAG GATTAGACGT AGTTGTTGGA TATTCGACCA ATCCGCAAGA ACTATTTAAA 
GTTTGCCAGC CGGGCGCTTT GTCGCTTGAT GCCAAACAAG TACTGAACAA CTTGCGTCAA 
CTGATAAATG ATTTGGAACA TATTAATATC AAAGATGAGC AAAACAGTGT GAATTTATTA 
CAGTCTGAAA CCTTGAAACC GCTTGAGCCA TCCGATGTGT TGAAAAGTCT GAGAAACTTG 
ATTTCAGACC TAATGATTCA CATTGAAATG TTAGGGGCAT TCGGTGGTCA CATTGCCTGT 
GTGGCGCATA TGATCCAAAT AGAACGTATA AAAAAGCACT GCCAGAACCA CCAACTCTTC 
ATAGTGTTGA ATTACGTCAT GACCATAATG GGGACGACAA AACTACTTCT GGAGGAAACC 
ATGGCCGGTT ATGAGCCGCT CGAAAAAATT CGAAAATTCT CCTCTGACAA AGTACTGAAA 
GTGTTTGAAA TTTTGGACGA ATACAAAACC AAATCGGACG AGGAACTTTG CTGTTTAGTC 
TTCACCAAAA GGCGGTTCAC AGCCAAGGTC CTCCACCACA TTATCGACAA AGCTAGCCAA 
GTTGATCCCA AATTCTATCA TATCAAGTCA AATTTCGTCG TTGGAAATAA GAATAATCCC 
TACAACGACA CCCGAGAGAA TTTATATATA ACAAAAAAGA ACCGAGAGGT GCTCAACAGC 
TTTGTCAGCA AGGAAATCAA CGTTTTGGTC AGCTCGAACG TGTTGGAGGA GGGAGTGGAC 
ATACCTAAGT GCACTCTCGT CATCAAATTC GACAAATCGG AGGACTACCG TTCGTATATC 
CAGTCGAAAG GACGCGCAAG ACATATCAAA AGTTTATACT ACACCATTGT AGAAACGACT 
GACGTAGCAA AGTACGATAA AAAATACTCC GCTTTCAAGG AAATCGAAAA CCTGGTGAAT 
GATCTTCTCA TCGGCAAAAA TTCTGAAAGA GATCATCCAA ACTTGTCGGA AATCCGCAAC 
ATGTATAATG AAGACAAGCT TGAGCCCTAC TACGTAAACG GCCCCAATTC GGCACAAGTA 
AACATGACCT CGGCCGTAGC TCTCTTGTGC CGTTACTGCA GTAATTTAGC AAGTGACAAA 
TACACCACTT ACGCCCCTGA GTGGTATTAT GAAGAGGATT CTAGCAGCGC AAAATTGCGG 
GTTGTGATAT TTTTGCCCGT TGTGTGCCCT CTTATCGACC CCATTGTCGG CCCTTACATG 
CACAACAAAA AAGACGCAAA AAGAGCAGCC GCACTAGTCG CATGTATAAA GCTACACCAG 
TGCGGCGAAC TTGACAATAA TTTGTTGCCA TGGAAGAAGC AGTTGGATGA AGCCGATGTT 
TCCTATCTTT TCACGCACTG GCCACAAGAA AAGGAAAGCG ACGCCGGAAA TAAGAAAAAG 
AAGAGGTTGC ACGATAAAGA GATCGCTCCG AGTGTGAAGA GTGCAATCCA GCCGGACAGA 
GTCCTCTATT TGCACACTAT TAACATAAAT CCGCAATACA AAAGAAGCGA CGATTTGAAA 
AACGCGGTCA CTATTTACGA CCTGTACAAA ACACCGTTAA AGTTTGGTTT GTTATCGCCG 
AAACCGTTGC CCGATTTGTG CAAATTTCCC CTCTTTGATT CAAATGGAAC GTTGGAAATT 
GAAATTAGGA ACAATGTTAG AGAGGTGGAG TTTGCAGCTA ACGAGATGAA GGAAATGAGG 
GAGTTCCATT TTTTGGTATT CAACGACCTT TTGGAGATTC TGAAAGAGTT TTTAATTTTC 
GACAACACTG GGATGAATTC GGAGATGTTG CTTGTTGTAC CGGTTCAGGA TCGGTGTGGG 
GATGTTTGCG TCGATTTTCG AGTGATTCGA GACAATAAAA ATTTGAAAAA TAAGCTCGAA 
CCGGCGGCTA CGGAACGCAT AAATTTGAAC GTAACTGAGG AAACTTACTT ACATAAGATT 
GTCTCTCCGT GGTATAGATC GCCACCAAAG ATGTACGTTG TGACGAAGGT TTGTCCTGAT 
AAGAGCGCCC TGAGCCGGTT TCCTAACCAC GAATATCCCA ATTTCGTGTC GTATTACTCC 
GAAAAACACT CTTTGTCGAT ATTGGACCCT TCGCAACCGC TGCTCTTGGT TAAAGGGCTT 
TCCGAACGGT TGAACGCTTT CAAACCGAGA GGTGCCGGTG GCAAGAGAAA GAAGGAAAAG 
ATGTATGAAG AATTGGAAGA ATATCTAATA CCAGAGCTGG TGATCAAGCA AGAATTTCCG 
TCGTGTTTGT GGATACAAGC GCGATTTTTG CCAAGCATTT TATCCCGCTT GGCTTACCTT 
CTAAAACTGC AACAACTCCA AGTTGACATA GCTCGTGGTA TTGGGGCCAA AGCCGAATAT 
TTGAAAGACT GTCCGCCGCT GGAGTTAAAC CTCCACTTAT TGCATTATGA GCCCAATGAC 
CCGCAACTAA CGCAAGAATC AGACAAGTCC ACTCCTTTGA TTGACAATTG CCTTGCGTTG 
GAGTGCCCGA AAAATTTGCG AACGATTCAA TACAATAAAG ATTTTGCGGC GAAAATGCTG 
GAAGCGGAGT ACTACTGGAA GACTATTGAA GAACCCAAAG ACATAGAGCG CAATATTAAC 
GTGACTGTTA TGGATATAGA GTATTACGAG ACTTTTATTT CGCATCAGCC TTCCAAAACC 
GGGCGTTTGT TGAAGAATGA CAGCCCGGTT AAGCAACAGA ATGTGCCCGC TATTACGTAC 
GATTGCCAAT TTGAAGCGAA ACAATTGCAA ATTCTGGACG TACAATTCGA CAATCAGAGC 
CCGAACTTGT GCCAGATTTA TCAAGCCTTG ACGGCAGCTG AAGCCAACGA TATCGTCAAC 
CTCGAACGGC TTGAAACCCT CGGCGACTCT TTCTTAAAAT TCGTAGCTTC CTTGTACATT 
ATTTTCAAAT TTCCGACCTA CAACGAGGGC AAATCGACCA CTCTCAAAGG GAAGCTGGTC 
AGCAACAAGA ATTTGTACTA CTTGGGAGTG AGGAAAAATC TGGGCGGTAT TTTGAAAAAC 
AGCGACTTGT CCCCGAGCGA TTGGGTCCCT CCGTGCTTTT GCATCCCACA AACAATCAGC 
AAAGCAATCG GCAATAAAGA GTATTCAGTA GTCTCGTTAT TTAACTGTTG CATTTCGCCG 
GAGGAGCAAG TTTCAGGTAA CTTGAACCGA AAGACTTTGA GCGATATGAC AACGGAAGAA 
ATTGCCCCGG ATGAGGAAAA TTCGTACGGC AATATGTGCA ACTTTTTGAA CAAACAATAC 
GTTGGTGATA AGAGCATAGC GGACTCTGTT GAGGCCCTCC TAGGCGCCTA CTTTCTAAGT 
GGCGGGATTC AAGGCGGGAT CAAATTCATG GAATGGATCG GCATTTTGCC CCTTTCTGAA 
CAAATCCAAC GCCTGATCGA AACCACTCAA GTCGACCCCG TCCTCAATAA AAAATCCACG 
AAAACTGATG TCGATTTCCA CATGCCACAG TGGCGCGAAA TCGAGCAACG TCTCGGTTAT 
ACGTTTACCA ACCGGGCGTT TTTACTCCAA GCTTTAACTC ATTCTTCCTA TTCGCCGAAT 
CGCATAACAC TGAGCTACGA ACGGTTGGAG TTTCTTGGTG ATGCTGTGCT TGATTTCCTC 
ATCACGTGTT ATATTTTCGA ACATTGTGGG CATTTGGAGC CGGGTCAAGT GACCGATTTG 
CGGTCATCCC TTGTCAACAA TAACACGTTC GCAAGCCTTG TTGTCCGATG CGGGTTTCAC 
AAGTTTTTGC TCATGATGAA TTCGAATTTG CAAGGTCATA TTGACAAGTT TGCCGATTAC 
CTTGCTTCAA AAAATTACGT GATCGACGAT GAAGTTCTGA TTTTGTTGGA GGAGGATGAG 
ATGAATATCG CCGAGTACGT CGATGTGCCT AAAGTTTTGG GTGATATTTT CGAAGCCCTT 
GCTGGCGCAA TTTATCTAGA TAGCAATAAG GATTTGAAGA CTGTTTGGAG GGTGTTTTAC 
AAGATTATCT GGCGCGAAAT TGATCTTTTT AGCAAGAATG TGCCAAAAAA TGTCATCAGG 
AGATTGTACG AGTGCCATAC TGTTTACCCT CCGCAATTTA GTAAAGCGTT GGAAGTTGGT 
AATCAAAAGA CTATGGTTTC TTTAGACTTT ATGTGTGAAG GTAGAAAGAA ACGAGTTCAT 
GGGTTTGGAA CGAACAAAAT CTTGGCTAAA AGGGCCGCTG CCAAAATTGC TCTTCGTGCT 
TTAAAGTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008201496.1
CDS	183..5054
Translation	MDEEDELKPRNYQVNLMEIAIRENTIIYLPTGSGKTFIAIMVLKQLCAPILRPYSDGGKISVILVNSVALVDQHGKYVRDHATFSVGTYTGEMNVDFWSEAEWEQQFNKYQVVIMTSQIMVNLINNRFIDLGKVNLMIFDECHHGVEDQPMRQIMKHFHSCTDKPRVLGLTATLLNGNCKLSKVMDEIRSLEVTFHSKVATVEGLDVVVGYSTNPQELFKVCQPGALSLDAKQVLNNLRQLINDLEHINIKDEQNSVNLLQSETLKPLEPSDVLKSLRNLISDLMIHIEMLGAFGGHIACVAHMIQIERIKKHCQNHQLFIVLNYVMTIMGTTKLLLEETMAGYEPLEKIRKFSSDKVLKVFEILDEYKTKSDEELCCLVFTKRRFTAKVLHHIIDKASQVDPKFYHIKSNFVVGNKNNPYNDTRENLYITKKNREVLNSFVSKEINVLVSSNVLEEGVDIPKCTLVIKFDKSEDYRSYIQSKGRARHIKSLYYTIVETTDVAKYDKKYSAFKEIENLVNDLLIGKNSERDHPNLSEIRNMYNEDKLEPYYVNGPNSAQVNMTSAVALLCRYCSNLASDKYTTYAPEWYYEEDSSSAKLRVVIFLPVVCPLIDPIVGPYMHNKKDAKRAAALVACIKLHQCGELDNNLLPWKKQLDEADVSYLFTHWPQEKESDAGNKKKKRLHDKEIAPSVKSAIQPDRVLYLHTININPQYKRSDDLKNAVTIYDLYKTPLKFGLLSPKPLPDLCKFPLFDSNGTLEIEIRNNVREVEFAANEMKEMREFHFLVFNDLLEILKEFLIFDNTGMNSEMLLVVPVQDRCGDVCVDFRVIRDNKNLKNKLEPAATERINLNVTEETYLHKIVSPWYRSPPKMYVVTKVCPDKSALSRFPNHEYPNFVSYYSEKHSLSILDPSQPLLLVKGLSERLNAFKPRGAGGKRKKEKMYEELEEYLIPELVIKQEFPSCLWIQARFLPSILSRLAYLLKLQQLQVDIARGIGAKAEYLKDCPPLELNLHLLHYEPNDPQLTQESDKSTPLIDNCLALECPKNLRTIQYNKDFAAKMLEAEYYWKTIEEPKDIERNINVTVMDIEYYETFISHQPSKTGRLLKNDSPVKQQNVPAITYDCQFEAKQLQILDVQFDNQSPNLCQIYQALTAAEANDIVNLERLETLGDSFLKFVASLYIIFKFPTYNEGKSTTLKGKLVSNKNLYYLGVRKNLGGILKNSDLSPSDWVPPCFCIPQTISKAIGNKEYSVVSLFNCCISPEEQVSGNLNRKTLSDMTTEEIAPDEENSYGNMCNFLNKQYVGDKSIADSVEALLGAYFLSGGIQGGIKFMEWIGILPLSEQIQRLIETTQVDPVLNKKSTKTDVDFHMPQWREIEQRLGYTFTNRAFLLQALTHSSYSPNRITLSYERLEFLGDAVLDFLITCYIFEHCGHLEPGQVTDLRSSLVNNNTFASLVVRCGFHKFLLMMNSNLQGHIDKFADYLASKNYVIDDEVLILLEEDEMNIAEYVDVPKVLGDIFEALAGAIYLDSNKDLKTVWRVFYKIIWREIDLFSKNVPKNVIRRLYECHTVYPPQFSKALEVGNQKTMVSLDFMCEGRKKRVHGFGTNKILAKRAAAKIALRALKL

Target ORF information:

RefSeq Version	XM_008203274.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum Dicer-2 (Dcr-2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008203274.2

ORF Insert Sequence:

ATGGACGAAG AAGACGAACT TAAACCTCGT AACTACCAAG TCAATTTGAT GGAAATTGCC 
ATTCGGGAAA ACACAATTAT TTATCTACCG ACTGGATCGG GTAAAACTTT TATTGCAATA 
ATGGTTTTGA AGCAGTTGTG CGCCCCCATA TTGAGACCCT ACAGCGATGG TGGAAAGATT 
TCAGTTATAT TGGTTAATTC TGTGGCTTTG GTGGATCAAC ATGGTAAGTA TGTAAGAGAC 
CACGCCACGT TCAGTGTGGG AACTTACACT GGAGAGATGA ATGTGGATTT TTGGAGCGAG 
GCAGAATGGG AACAGCAATT TAATAAGTAT CAAGTTGTTA TAATGACCAG CCAAATCATG 
GTAAATCTCA TCAACAACCG CTTCATAGAT TTGGGAAAAG TAAACTTGAT GATTTTCGAT 
GAATGCCACC ATGGTGTGGA AGACCAGCCA ATGCGCCAGA TAATGAAACA CTTCCACAGC 
TGTACCGACA AGCCTCGAGT TCTTGGTCTT ACAGCCACGT TATTAAATGG CAATTGCAAA 
TTGTCGAAAG TCATGGATGA AATAAGGTCT TTGGAGGTGA CCTTCCACAG TAAAGTGGCC 
ACTGTGGAAG GATTAGACGT AGTTGTTGGA TATTCGACCA ATCCGCAAGA ACTATTTAAA 
GTTTGCCAGC CGGGCGCTTT GTCGCTTGAT GCCAAACAAG TACTGAACAA CTTGCGTCAA 
CTGATAAATG ATTTGGAACA TATTAATATC AAAGATGAGC AAAACAGTGT GAATTTATTA 
CAGTCTGAAA CCTTGAAACC GCTTGAGCCA TCCGATGTGT TGAAAAGTCT GAGAAACTTG 
ATTTCAGACC TAATGATTCA CATTGAAATG TTAGGGGCAT TCGGTGGTCA CATTGCCTGT 
GTGGCGCATA TGATCCAAAT AGAACGTATA AAAAAGCACT GCCAGAACCA CCAACTCTTC 
ATAGTGTTGA ATTACGTCAT GACCATAATG GGGACGACAA AACTACTTCT GGAGGAAACC 
ATGGCCGGTT ATGAGCCGCT CGAAAAAATT CGAAAATTCT CCTCTGACAA AGTACTGAAA 
GTGTTTGAAA TTTTGGACGA ATACAAAACC AAATCGGACG AGGAACTTTG CTGTTTAGTC 
TTCACCAAAA GGCGGTTCAC AGCCAAGGTC CTCCACCACA TTATCGACAA AGCTAGCCAA 
GTTGATCCCA AATTCTATCA TATCAAGTCA AATTTCGTCG TTGGAAATAA GAATAATCCC 
TACAACGACA CCCGAGAGAA TTTATATATA ACAAAAAAGA ACCGAGAGGT GCTCAACAGC 
TTTGTCAGCA AGGAAATCAA CGTTTTGGTC AGCTCGAACG TGTTGGAGGA GGGAGTGGAC 
ATACCTAAGT GCACTCTCGT CATCAAATTC GACAAATCGG AGGACTACCG TTCGTATATC 
CAGTCGAAAG GACGCGCAAG ACATATCAAA AGTTTATACT ACACCATTGT AGAAACGACT 
GACGTAGCAA AGTACGATAA AAAATACTCC GCTTTCAAGG AAATCGAAAA CCTGGTGAAT 
GATCTTCTCA TCGGCAAAAA TTCTGAAAGA GATCATCCAA ACTTGTCGGA AATCCGCAAC 
ATGTATAATG AAGACAAGCT TGAGCCCTAC TACGTAAACG GCCCCAATTC GGCACAAGTA 
AACATGACCT CGGCCGTAGC TCTCTTGTGC CGTTACTGCA GTAATTTAGC AAGTGACAAA 
TACACCACTT ACGCCCCTGA GTGGTATTAT GAAGAGGATT CTAGCAGCGC AAAATTGCGG 
GTTGTGATAT TTTTGCCCGT TGTGTGCCCT CTTATCGACC CCATTGTCGG CCCTTACATG 
CACAACAAAA AAGACGCAAA AAGAGCAGCC GCACTAGTCG CATGTATAAA GCTACACCAG 
TGCGGCGAAC TTGACAATAA TTTGTTGCCA TGGAAGAAGC AGTTGGATGA AGCCGATGTT 
TCCTATCTTT TCACGCACTG GCCACAAGAA AAGGAAAGCG ACGCCGGAAA TAAGAAAAAG 
AAGAGGTTGC ACGATAAAGA GATCGCTCCG AGTGTGAAGA GTGCAATCCA GCCGGACAGA 
GTCCTCTATT TGCACACTAT TAACATAAAT CCGCAATACA AAAGAAGCGA CGATTTGAAA 
AACGCGGTCA CTATTTACGA CCTGTACAAA ACACCGTTAA AGTTTGGTTT GTTATCGCCG 
AAACCGTTGC CCGATTTGTG CAAATTTCCC CTCTTTGATT CAAATGGAAC GTTGGAAATT 
GAAATTAGGA ACAATGTTAG AGAGGTGGAG TTTGCAGCTA ACGAGATGAA GGAAATGAGG 
GAGTTCCATT TTTTGGTATT CAACGACCTT TTGGAGATTC TGAAAGAGTT TTTAATTTTC 
GACAACACTG GGATGAATTC GGAGATGTTG CTTGTTGTAC CGGTTCAGGA TCGGTGTGGG 
GATGTTTGCG TCGATTTTCG AGTGATTCGA GACAATAAAA ATTTGAAAAA TAAGCTCGAA 
CCGGCGGCTA CGGAACGCAT AAATTTGAAC GTAACTGAGG AAACTTACTT ACATAAGATT 
GTCTCTCCGT GGTATAGATC GCCACCAAAG ATGTACGTTG TGACGAAGGT TTGTCCTGAT 
AAGAGCGCCC TGAGCCGGTT TCCTAACCAC GAATATCCCA ATTTCGTGTC GTATTACTCC 
GAAAAACACT CTTTGTCGAT ATTGGACCCT TCGCAACCGC TGCTCTTGGT TAAAGGGCTT 
TCCGAACGGT TGAACGCTTT CAAACCGAGA GGTGCCGGTG GCAAGAGAAA GAAGGAAAAG 
ATGTATGAAG AATTGGAAGA ATATCTAATA CCAGAGCTGG TGATCAAGCA AGAATTTCCG 
TCGTGTTTGT GGATACAAGC GCGATTTTTG CCAAGCATTT TATCCCGCTT GGCTTACCTT 
CTAAAACTGC AACAACTCCA AGTTGACATA GCTCGTGGTA TTGGGGCCAA AGCCGAATAT 
TTGAAAGACT GTCCGCCGCT GGAGTTAAAC CTCCACTTAT TGCATTATGA GCCCAATGAC 
CCGCAACTAA CGCAAGAATC AGACAAGTCC ACTCCTTTGA TTGACAATTG CCTTGCGTTG 
GAGTGCCCGA AAAATTTGCG AACGATTCAA TACAATAAAG ATTTTGCGGC GAAAATGCTG 
GAAGCGGAGT ACTACTGGAA GACTATTGAA GAACCCAAAG ACATAGAGCG CAATATTAAC 
GTGACTGTTA TGGATATAGA GTATTACGAG ACTTTTATTT CGCATCAGCC TTCCAAAACC 
GGGCGTTTGT TGAAGAATGA CAGCCCGGTT AAGCAACAGA ATGTGCCCGC TATTACGTAC 
GATTGCCAAT TTGAAGCGAA ACAATTGCAA ATTCTGGACG TACAATTCGA CAATCAGAGC 
CCGAACTTGT GCCAGATTTA TCAAGCCTTG ACGGCAGCTG AAGCCAACGA TATCGTCAAC 
CTCGAACGGC TTGAAACCCT CGGCGACTCT TTCTTAAAAT TCGTAGCTTC CTTGTACATT 
ATTTTCAAAT TTCCGACCTA CAACGAGGGC AAATCGACCA CTCTCAAAGG GAAGCTGGTC 
AGCAACAAGA ATTTGTACTA CTTGGGAGTG AGGAAAAATC TGGGCGGTAT TTTGAAAAAC 
AGCGACTTGT CCCCGAGCGA TTGGGTCCCT CCGTGCTTTT GCATCCCACA AACAATCAGC 
AAAGCAATCG GCAATAAAGA GTATTCAGTA GTCTCGTTAT TTAACTGTTG CATTTCGCCG 
GAGGAGCAAG TTTCAGGTAA CTTGAACCGA AAGACTTTGA GCGATATGAC AACGGAAGAA 
ATTGCCCCGG ATGAGGAAAA TTCGTACGGC AATATGTGCA ACTTTTTGAA CAAACAATAC 
GTTGGTGATA AGAGCATAGC GGACTCTGTT GAGGCCCTCC TAGGCGCCTA CTTTCTAAGT 
GGCGGGATTC AAGGCGGGAT CAAATTCATG GAATGGATCG GCATTTTGCC CCTTTCTGAA 
CAAATCCAAC GCCTGATCGA AACCACTCAA GTCGACCCCG TCCTCAATAA AAAATCCACG 
AAAACTGATG TCGATTTCCA CATGCCACAG TGGCGCGAAA TCGAGCAACG TCTCGGTTAT 
ACGTTTACCA ACCGGGCGTT TTTACTCCAA GCTTTAACTC ATTCTTCCTA TTCGCCGAAT 
CGCATAACAC TGAGCTACGA ACGGTTGGAG TTTCTTGGTG ATGCTGTGCT TGATTTCCTC 
ATCACGTGTT ATATTTTCGA ACATTGTGGG CATTTGGAGC CGGGTCAAGT GACCGATTTG 
CGGTCATCCC TTGTCAACAA TAACACGTTC GCAAGCCTTG TTGTCCGATG CGGGTTTCAC 
AAGTTTTTGC TCATGATGAA TTCGAATTTG CAAGGTCATA TTGACAAGTT TGCCGATTAC 
CTTGCTTCAA AAAATTACGT GATCGACGAT GAAGTTCTGA TTTTGTTGGA GGAGGATGAG 
ATGAATATCG CCGAGTACGT CGATGTGCCT AAAGTTTTGG GTGATATTTT CGAAGCCCTT 
GCTGGCGCAA TTTATCTAGA TAGCAATAAG GATTTGAAGA CTGTTTGGAG GGTGTTTTAC 
AAGATTATCT GGCGCGAAAT TGATCTTTTT AGCAAGAATG TGCCAAAAAA TGTCATCAGG 
AGATTGTACG AGTGCCATAC TGTTTACCCT CCGCAATTTA GTAAAGCGTT GGAAGTTGGT 
AATCAAAAGA CTATGGTTTC TTTAGACTTT ATGTGTGAAG GTAGAAAGAA ACGAGTTCAT 
GGGTTTGGAA CGAACAAAAT CTTGGCTAAA AGGGCCGCTG CCAAAATTGC TCTTCGTGCT 
TTAAAGTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dcr-2 ( XM_015984027.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015839513.1 Tribolium castaneum Dicer-2 (Dcr-2), transcript variant X2, mRNA.

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CloneID	OTg01906
Clone ID Related Accession (Same CDS sequence)	XM_008203274.2 , XM_015984027.1 , NM_001114368.1
Accession Version	XM_015984027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	dicer-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450135.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAAG AAGACGAACT TAAACCTCGT AACTACCAAG TCAATTTGAT GGAAATTGCC 
ATTCGGGAAA ACACAATTAT TTATCTACCG ACTGGATCGG GTAAAACTTT TATTGCAATA 
ATGGTTTTGA AGCAGTTGTG CGCCCCCATA TTGAGACCCT ACAGCGATGG TGGAAAGATT 
TCAGTTATAT TGGTTAATTC TGTGGCTTTG GTGGATCAAC ATGGTAAGTA TGTAAGAGAC 
CACGCCACGT TCAGTGTGGG AACTTACACT GGAGAGATGA ATGTGGATTT TTGGAGCGAG 
GCAGAATGGG AACAGCAATT TAATAAGTAT CAAGTTGTTA TAATGACCAG CCAAATCATG 
GTAAATCTCA TCAACAACCG CTTCATAGAT TTGGGAAAAG TAAACTTGAT GATTTTCGAT 
GAATGCCACC ATGGTGTGGA AGACCAGCCA ATGCGCCAGA TAATGAAACA CTTCCACAGC 
TGTACCGACA AGCCTCGAGT TCTTGGTCTT ACAGCCACGT TATTAAATGG CAATTGCAAA 
TTGTCGAAAG TCATGGATGA AATAAGGTCT TTGGAGGTGA CCTTCCACAG TAAAGTGGCC 
ACTGTGGAAG GATTAGACGT AGTTGTTGGA TATTCGACCA ATCCGCAAGA ACTATTTAAA 
GTTTGCCAGC CGGGCGCTTT GTCGCTTGAT GCCAAACAAG TACTGAACAA CTTGCGTCAA 
CTGATAAATG ATTTGGAACA TATTAATATC AAAGATGAGC AAAACAGTGT GAATTTATTA 
CAGTCTGAAA CCTTGAAACC GCTTGAGCCA TCCGATGTGT TGAAAAGTCT GAGAAACTTG 
ATTTCAGACC TAATGATTCA CATTGAAATG TTAGGGGCAT TCGGTGGTCA CATTGCCTGT 
GTGGCGCATA TGATCCAAAT AGAACGTATA AAAAAGCACT GCCAGAACCA CCAACTCTTC 
ATAGTGTTGA ATTACGTCAT GACCATAATG GGGACGACAA AACTACTTCT GGAGGAAACC 
ATGGCCGGTT ATGAGCCGCT CGAAAAAATT CGAAAATTCT CCTCTGACAA AGTACTGAAA 
GTGTTTGAAA TTTTGGACGA ATACAAAACC AAATCGGACG AGGAACTTTG CTGTTTAGTC 
TTCACCAAAA GGCGGTTCAC AGCCAAGGTC CTCCACCACA TTATCGACAA AGCTAGCCAA 
GTTGATCCCA AATTCTATCA TATCAAGTCA AATTTCGTCG TTGGAAATAA GAATAATCCC 
TACAACGACA CCCGAGAGAA TTTATATATA ACAAAAAAGA ACCGAGAGGT GCTCAACAGC 
TTTGTCAGCA AGGAAATCAA CGTTTTGGTC AGCTCGAACG TGTTGGAGGA GGGAGTGGAC 
ATACCTAAGT GCACTCTCGT CATCAAATTC GACAAATCGG AGGACTACCG TTCGTATATC 
CAGTCGAAAG GACGCGCAAG ACATATCAAA AGTTTATACT ACACCATTGT AGAAACGACT 
GACGTAGCAA AGTACGATAA AAAATACTCC GCTTTCAAGG AAATCGAAAA CCTGGTGAAT 
GATCTTCTCA TCGGCAAAAA TTCTGAAAGA GATCATCCAA ACTTGTCGGA AATCCGCAAC 
ATGTATAATG AAGACAAGCT TGAGCCCTAC TACGTAAACG GCCCCAATTC GGCACAAGTA 
AACATGACCT CGGCCGTAGC TCTCTTGTGC CGTTACTGCA GTAATTTAGC AAGTGACAAA 
TACACCACTT ACGCCCCTGA GTGGTATTAT GAAGAGGATT CTAGCAGCGC AAAATTGCGG 
GTTGTGATAT TTTTGCCCGT TGTGTGCCCT CTTATCGACC CCATTGTCGG CCCTTACATG 
CACAACAAAA AAGACGCAAA AAGAGCAGCC GCACTAGTCG CATGTATAAA GCTACACCAG 
TGCGGCGAAC TTGACAATAA TTTGTTGCCA TGGAAGAAGC AGTTGGATGA AGCCGATGTT 
TCCTATCTTT TCACGCACTG GCCACAAGAA AAGGAAAGCG ACGCCGGAAA TAAGAAAAAG 
AAGAGGTTGC ACGATAAAGA GATCGCTCCG AGTGTGAAGA GTGCAATCCA GCCGGACAGA 
GTCCTCTATT TGCACACTAT TAACATAAAT CCGCAATACA AAAGAAGCGA CGATTTGAAA 
AACGCGGTCA CTATTTACGA CCTGTACAAA ACACCGTTAA AGTTTGGTTT GTTATCGCCG 
AAACCGTTGC CCGATTTGTG CAAATTTCCC CTCTTTGATT CAAATGGAAC GTTGGAAATT 
GAAATTAGGA ACAATGTTAG AGAGGTGGAG TTTGCAGCTA ACGAGATGAA GGAAATGAGG 
GAGTTCCATT TTTTGGTATT CAACGACCTT TTGGAGATTC TGAAAGAGTT TTTAATTTTC 
GACAACACTG GGATGAATTC GGAGATGTTG CTTGTTGTAC CGGTTCAGGA TCGGTGTGGG 
GATGTTTGCG TCGATTTTCG AGTGATTCGA GACAATAAAA ATTTGAAAAA TAAGCTCGAA 
CCGGCGGCTA CGGAACGCAT AAATTTGAAC GTAACTGAGG AAACTTACTT ACATAAGATT 
GTCTCTCCGT GGTATAGATC GCCACCAAAG ATGTACGTTG TGACGAAGGT TTGTCCTGAT 
AAGAGCGCCC TGAGCCGGTT TCCTAACCAC GAATATCCCA ATTTCGTGTC GTATTACTCC 
GAAAAACACT CTTTGTCGAT ATTGGACCCT TCGCAACCGC TGCTCTTGGT TAAAGGGCTT 
TCCGAACGGT TGAACGCTTT CAAACCGAGA GGTGCCGGTG GCAAGAGAAA GAAGGAAAAG 
ATGTATGAAG AATTGGAAGA ATATCTAATA CCAGAGCTGG TGATCAAGCA AGAATTTCCG 
TCGTGTTTGT GGATACAAGC GCGATTTTTG CCAAGCATTT TATCCCGCTT GGCTTACCTT 
CTAAAACTGC AACAACTCCA AGTTGACATA GCTCGTGGTA TTGGGGCCAA AGCCGAATAT 
TTGAAAGACT GTCCGCCGCT GGAGTTAAAC CTCCACTTAT TGCATTATGA GCCCAATGAC 
CCGCAACTAA CGCAAGAATC AGACAAGTCC ACTCCTTTGA TTGACAATTG CCTTGCGTTG 
GAGTGCCCGA AAAATTTGCG AACGATTCAA TACAATAAAG ATTTTGCGGC GAAAATGCTG 
GAAGCGGAGT ACTACTGGAA GACTATTGAA GAACCCAAAG ACATAGAGCG CAATATTAAC 
GTGACTGTTA TGGATATAGA GTATTACGAG ACTTTTATTT CGCATCAGCC TTCCAAAACC 
GGGCGTTTGT TGAAGAATGA CAGCCCGGTT AAGCAACAGA ATGTGCCCGC TATTACGTAC 
GATTGCCAAT TTGAAGCGAA ACAATTGCAA ATTCTGGACG TACAATTCGA CAATCAGAGC 
CCGAACTTGT GCCAGATTTA TCAAGCCTTG ACGGCAGCTG AAGCCAACGA TATCGTCAAC 
CTCGAACGGC TTGAAACCCT CGGCGACTCT TTCTTAAAAT TCGTAGCTTC CTTGTACATT 
ATTTTCAAAT TTCCGACCTA CAACGAGGGC AAATCGACCA CTCTCAAAGG GAAGCTGGTC 
AGCAACAAGA ATTTGTACTA CTTGGGAGTG AGGAAAAATC TGGGCGGTAT TTTGAAAAAC 
AGCGACTTGT CCCCGAGCGA TTGGGTCCCT CCGTGCTTTT GCATCCCACA AACAATCAGC 
AAAGCAATCG GCAATAAAGA GTATTCAGTA GTCTCGTTAT TTAACTGTTG CATTTCGCCG 
GAGGAGCAAG TTTCAGGTAA CTTGAACCGA AAGACTTTGA GCGATATGAC AACGGAAGAA 
ATTGCCCCGG ATGAGGAAAA TTCGTACGGC AATATGTGCA ACTTTTTGAA CAAACAATAC 
GTTGGTGATA AGAGCATAGC GGACTCTGTT GAGGCCCTCC TAGGCGCCTA CTTTCTAAGT 
GGCGGGATTC AAGGCGGGAT CAAATTCATG GAATGGATCG GCATTTTGCC CCTTTCTGAA 
CAAATCCAAC GCCTGATCGA AACCACTCAA GTCGACCCCG TCCTCAATAA AAAATCCACG 
AAAACTGATG TCGATTTCCA CATGCCACAG TGGCGCGAAA TCGAGCAACG TCTCGGTTAT 
ACGTTTACCA ACCGGGCGTT TTTACTCCAA GCTTTAACTC ATTCTTCCTA TTCGCCGAAT 
CGCATAACAC TGAGCTACGA ACGGTTGGAG TTTCTTGGTG ATGCTGTGCT TGATTTCCTC 
ATCACGTGTT ATATTTTCGA ACATTGTGGG CATTTGGAGC CGGGTCAAGT GACCGATTTG 
CGGTCATCCC TTGTCAACAA TAACACGTTC GCAAGCCTTG TTGTCCGATG CGGGTTTCAC 
AAGTTTTTGC TCATGATGAA TTCGAATTTG CAAGGTCATA TTGACAAGTT TGCCGATTAC 
CTTGCTTCAA AAAATTACGT GATCGACGAT GAAGTTCTGA TTTTGTTGGA GGAGGATGAG 
ATGAATATCG CCGAGTACGT CGATGTGCCT AAAGTTTTGG GTGATATTTT CGAAGCCCTT 
GCTGGCGCAA TTTATCTAGA TAGCAATAAG GATTTGAAGA CTGTTTGGAG GGTGTTTTAC 
AAGATTATCT GGCGCGAAAT TGATCTTTTT AGCAAGAATG TGCCAAAAAA TGTCATCAGG 
AGATTGTACG AGTGCCATAC TGTTTACCCT CCGCAATTTA GTAAAGCGTT GGAAGTTGGT 
AATCAAAAGA CTATGGTTTC TTTAGACTTT ATGTGTGAAG GTAGAAAGAA ACGAGTTCAT 
GGGTTTGGAA CGAACAAAAT CTTGGCTAAA AGGGCCGCTG CCAAAATTGC TCTTCGTGCT 
TTAAAGTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015839513.1
CDS	82..4953
Translation	MDEEDELKPRNYQVNLMEIAIRENTIIYLPTGSGKTFIAIMVLKQLCAPILRPYSDGGKISVILVNSVALVDQHGKYVRDHATFSVGTYTGEMNVDFWSEAEWEQQFNKYQVVIMTSQIMVNLINNRFIDLGKVNLMIFDECHHGVEDQPMRQIMKHFHSCTDKPRVLGLTATLLNGNCKLSKVMDEIRSLEVTFHSKVATVEGLDVVVGYSTNPQELFKVCQPGALSLDAKQVLNNLRQLINDLEHINIKDEQNSVNLLQSETLKPLEPSDVLKSLRNLISDLMIHIEMLGAFGGHIACVAHMIQIERIKKHCQNHQLFIVLNYVMTIMGTTKLLLEETMAGYEPLEKIRKFSSDKVLKVFEILDEYKTKSDEELCCLVFTKRRFTAKVLHHIIDKASQVDPKFYHIKSNFVVGNKNNPYNDTRENLYITKKNREVLNSFVSKEINVLVSSNVLEEGVDIPKCTLVIKFDKSEDYRSYIQSKGRARHIKSLYYTIVETTDVAKYDKKYSAFKEIENLVNDLLIGKNSERDHPNLSEIRNMYNEDKLEPYYVNGPNSAQVNMTSAVALLCRYCSNLASDKYTTYAPEWYYEEDSSSAKLRVVIFLPVVCPLIDPIVGPYMHNKKDAKRAAALVACIKLHQCGELDNNLLPWKKQLDEADVSYLFTHWPQEKESDAGNKKKKRLHDKEIAPSVKSAIQPDRVLYLHTININPQYKRSDDLKNAVTIYDLYKTPLKFGLLSPKPLPDLCKFPLFDSNGTLEIEIRNNVREVEFAANEMKEMREFHFLVFNDLLEILKEFLIFDNTGMNSEMLLVVPVQDRCGDVCVDFRVIRDNKNLKNKLEPAATERINLNVTEETYLHKIVSPWYRSPPKMYVVTKVCPDKSALSRFPNHEYPNFVSYYSEKHSLSILDPSQPLLLVKGLSERLNAFKPRGAGGKRKKEKMYEELEEYLIPELVIKQEFPSCLWIQARFLPSILSRLAYLLKLQQLQVDIARGIGAKAEYLKDCPPLELNLHLLHYEPNDPQLTQESDKSTPLIDNCLALECPKNLRTIQYNKDFAAKMLEAEYYWKTIEEPKDIERNINVTVMDIEYYETFISHQPSKTGRLLKNDSPVKQQNVPAITYDCQFEAKQLQILDVQFDNQSPNLCQIYQALTAAEANDIVNLERLETLGDSFLKFVASLYIIFKFPTYNEGKSTTLKGKLVSNKNLYYLGVRKNLGGILKNSDLSPSDWVPPCFCIPQTISKAIGNKEYSVVSLFNCCISPEEQVSGNLNRKTLSDMTTEEIAPDEENSYGNMCNFLNKQYVGDKSIADSVEALLGAYFLSGGIQGGIKFMEWIGILPLSEQIQRLIETTQVDPVLNKKSTKTDVDFHMPQWREIEQRLGYTFTNRAFLLQALTHSSYSPNRITLSYERLEFLGDAVLDFLITCYIFEHCGHLEPGQVTDLRSSLVNNNTFASLVVRCGFHKFLLMMNSNLQGHIDKFADYLASKNYVIDDEVLILLEEDEMNIAEYVDVPKVLGDIFEALAGAIYLDSNKDLKTVWRVFYKIIWREIDLFSKNVPKNVIRRLYECHTVYPPQFSKALEVGNQKTMVSLDFMCEGRKKRVHGFGTNKILAKRAAAKIALRALKL

Target ORF information:

RefSeq Version	XM_015984027.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum Dicer-2 (Dcr-2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015984027.1

ORF Insert Sequence:

ATGGACGAAG AAGACGAACT TAAACCTCGT AACTACCAAG TCAATTTGAT GGAAATTGCC 
ATTCGGGAAA ACACAATTAT TTATCTACCG ACTGGATCGG GTAAAACTTT TATTGCAATA 
ATGGTTTTGA AGCAGTTGTG CGCCCCCATA TTGAGACCCT ACAGCGATGG TGGAAAGATT 
TCAGTTATAT TGGTTAATTC TGTGGCTTTG GTGGATCAAC ATGGTAAGTA TGTAAGAGAC 
CACGCCACGT TCAGTGTGGG AACTTACACT GGAGAGATGA ATGTGGATTT TTGGAGCGAG 
GCAGAATGGG AACAGCAATT TAATAAGTAT CAAGTTGTTA TAATGACCAG CCAAATCATG 
GTAAATCTCA TCAACAACCG CTTCATAGAT TTGGGAAAAG TAAACTTGAT GATTTTCGAT 
GAATGCCACC ATGGTGTGGA AGACCAGCCA ATGCGCCAGA TAATGAAACA CTTCCACAGC 
TGTACCGACA AGCCTCGAGT TCTTGGTCTT ACAGCCACGT TATTAAATGG CAATTGCAAA 
TTGTCGAAAG TCATGGATGA AATAAGGTCT TTGGAGGTGA CCTTCCACAG TAAAGTGGCC 
ACTGTGGAAG GATTAGACGT AGTTGTTGGA TATTCGACCA ATCCGCAAGA ACTATTTAAA 
GTTTGCCAGC CGGGCGCTTT GTCGCTTGAT GCCAAACAAG TACTGAACAA CTTGCGTCAA 
CTGATAAATG ATTTGGAACA TATTAATATC AAAGATGAGC AAAACAGTGT GAATTTATTA 
CAGTCTGAAA CCTTGAAACC GCTTGAGCCA TCCGATGTGT TGAAAAGTCT GAGAAACTTG 
ATTTCAGACC TAATGATTCA CATTGAAATG TTAGGGGCAT TCGGTGGTCA CATTGCCTGT 
GTGGCGCATA TGATCCAAAT AGAACGTATA AAAAAGCACT GCCAGAACCA CCAACTCTTC 
ATAGTGTTGA ATTACGTCAT GACCATAATG GGGACGACAA AACTACTTCT GGAGGAAACC 
ATGGCCGGTT ATGAGCCGCT CGAAAAAATT CGAAAATTCT CCTCTGACAA AGTACTGAAA 
GTGTTTGAAA TTTTGGACGA ATACAAAACC AAATCGGACG AGGAACTTTG CTGTTTAGTC 
TTCACCAAAA GGCGGTTCAC AGCCAAGGTC CTCCACCACA TTATCGACAA AGCTAGCCAA 
GTTGATCCCA AATTCTATCA TATCAAGTCA AATTTCGTCG TTGGAAATAA GAATAATCCC 
TACAACGACA CCCGAGAGAA TTTATATATA ACAAAAAAGA ACCGAGAGGT GCTCAACAGC 
TTTGTCAGCA AGGAAATCAA CGTTTTGGTC AGCTCGAACG TGTTGGAGGA GGGAGTGGAC 
ATACCTAAGT GCACTCTCGT CATCAAATTC GACAAATCGG AGGACTACCG TTCGTATATC 
CAGTCGAAAG GACGCGCAAG ACATATCAAA AGTTTATACT ACACCATTGT AGAAACGACT 
GACGTAGCAA AGTACGATAA AAAATACTCC GCTTTCAAGG AAATCGAAAA CCTGGTGAAT 
GATCTTCTCA TCGGCAAAAA TTCTGAAAGA GATCATCCAA ACTTGTCGGA AATCCGCAAC 
ATGTATAATG AAGACAAGCT TGAGCCCTAC TACGTAAACG GCCCCAATTC GGCACAAGTA 
AACATGACCT CGGCCGTAGC TCTCTTGTGC CGTTACTGCA GTAATTTAGC AAGTGACAAA 
TACACCACTT ACGCCCCTGA GTGGTATTAT GAAGAGGATT CTAGCAGCGC AAAATTGCGG 
GTTGTGATAT TTTTGCCCGT TGTGTGCCCT CTTATCGACC CCATTGTCGG CCCTTACATG 
CACAACAAAA AAGACGCAAA AAGAGCAGCC GCACTAGTCG CATGTATAAA GCTACACCAG 
TGCGGCGAAC TTGACAATAA TTTGTTGCCA TGGAAGAAGC AGTTGGATGA AGCCGATGTT 
TCCTATCTTT TCACGCACTG GCCACAAGAA AAGGAAAGCG ACGCCGGAAA TAAGAAAAAG 
AAGAGGTTGC ACGATAAAGA GATCGCTCCG AGTGTGAAGA GTGCAATCCA GCCGGACAGA 
GTCCTCTATT TGCACACTAT TAACATAAAT CCGCAATACA AAAGAAGCGA CGATTTGAAA 
AACGCGGTCA CTATTTACGA CCTGTACAAA ACACCGTTAA AGTTTGGTTT GTTATCGCCG 
AAACCGTTGC CCGATTTGTG CAAATTTCCC CTCTTTGATT CAAATGGAAC GTTGGAAATT 
GAAATTAGGA ACAATGTTAG AGAGGTGGAG TTTGCAGCTA ACGAGATGAA GGAAATGAGG 
GAGTTCCATT TTTTGGTATT CAACGACCTT TTGGAGATTC TGAAAGAGTT TTTAATTTTC 
GACAACACTG GGATGAATTC GGAGATGTTG CTTGTTGTAC CGGTTCAGGA TCGGTGTGGG 
GATGTTTGCG TCGATTTTCG AGTGATTCGA GACAATAAAA ATTTGAAAAA TAAGCTCGAA 
CCGGCGGCTA CGGAACGCAT AAATTTGAAC GTAACTGAGG AAACTTACTT ACATAAGATT 
GTCTCTCCGT GGTATAGATC GCCACCAAAG ATGTACGTTG TGACGAAGGT TTGTCCTGAT 
AAGAGCGCCC TGAGCCGGTT TCCTAACCAC GAATATCCCA ATTTCGTGTC GTATTACTCC 
GAAAAACACT CTTTGTCGAT ATTGGACCCT TCGCAACCGC TGCTCTTGGT TAAAGGGCTT 
TCCGAACGGT TGAACGCTTT CAAACCGAGA GGTGCCGGTG GCAAGAGAAA GAAGGAAAAG 
ATGTATGAAG AATTGGAAGA ATATCTAATA CCAGAGCTGG TGATCAAGCA AGAATTTCCG 
TCGTGTTTGT GGATACAAGC GCGATTTTTG CCAAGCATTT TATCCCGCTT GGCTTACCTT 
CTAAAACTGC AACAACTCCA AGTTGACATA GCTCGTGGTA TTGGGGCCAA AGCCGAATAT 
TTGAAAGACT GTCCGCCGCT GGAGTTAAAC CTCCACTTAT TGCATTATGA GCCCAATGAC 
CCGCAACTAA CGCAAGAATC AGACAAGTCC ACTCCTTTGA TTGACAATTG CCTTGCGTTG 
GAGTGCCCGA AAAATTTGCG AACGATTCAA TACAATAAAG ATTTTGCGGC GAAAATGCTG 
GAAGCGGAGT ACTACTGGAA GACTATTGAA GAACCCAAAG ACATAGAGCG CAATATTAAC 
GTGACTGTTA TGGATATAGA GTATTACGAG ACTTTTATTT CGCATCAGCC TTCCAAAACC 
GGGCGTTTGT TGAAGAATGA CAGCCCGGTT AAGCAACAGA ATGTGCCCGC TATTACGTAC 
GATTGCCAAT TTGAAGCGAA ACAATTGCAA ATTCTGGACG TACAATTCGA CAATCAGAGC 
CCGAACTTGT GCCAGATTTA TCAAGCCTTG ACGGCAGCTG AAGCCAACGA TATCGTCAAC 
CTCGAACGGC TTGAAACCCT CGGCGACTCT TTCTTAAAAT TCGTAGCTTC CTTGTACATT 
ATTTTCAAAT TTCCGACCTA CAACGAGGGC AAATCGACCA CTCTCAAAGG GAAGCTGGTC 
AGCAACAAGA ATTTGTACTA CTTGGGAGTG AGGAAAAATC TGGGCGGTAT TTTGAAAAAC 
AGCGACTTGT CCCCGAGCGA TTGGGTCCCT CCGTGCTTTT GCATCCCACA AACAATCAGC 
AAAGCAATCG GCAATAAAGA GTATTCAGTA GTCTCGTTAT TTAACTGTTG CATTTCGCCG 
GAGGAGCAAG TTTCAGGTAA CTTGAACCGA AAGACTTTGA GCGATATGAC AACGGAAGAA 
ATTGCCCCGG ATGAGGAAAA TTCGTACGGC AATATGTGCA ACTTTTTGAA CAAACAATAC 
GTTGGTGATA AGAGCATAGC GGACTCTGTT GAGGCCCTCC TAGGCGCCTA CTTTCTAAGT 
GGCGGGATTC AAGGCGGGAT CAAATTCATG GAATGGATCG GCATTTTGCC CCTTTCTGAA 
CAAATCCAAC GCCTGATCGA AACCACTCAA GTCGACCCCG TCCTCAATAA AAAATCCACG 
AAAACTGATG TCGATTTCCA CATGCCACAG TGGCGCGAAA TCGAGCAACG TCTCGGTTAT 
ACGTTTACCA ACCGGGCGTT TTTACTCCAA GCTTTAACTC ATTCTTCCTA TTCGCCGAAT 
CGCATAACAC TGAGCTACGA ACGGTTGGAG TTTCTTGGTG ATGCTGTGCT TGATTTCCTC 
ATCACGTGTT ATATTTTCGA ACATTGTGGG CATTTGGAGC CGGGTCAAGT GACCGATTTG 
CGGTCATCCC TTGTCAACAA TAACACGTTC GCAAGCCTTG TTGTCCGATG CGGGTTTCAC 
AAGTTTTTGC TCATGATGAA TTCGAATTTG CAAGGTCATA TTGACAAGTT TGCCGATTAC 
CTTGCTTCAA AAAATTACGT GATCGACGAT GAAGTTCTGA TTTTGTTGGA GGAGGATGAG 
ATGAATATCG CCGAGTACGT CGATGTGCCT AAAGTTTTGG GTGATATTTT CGAAGCCCTT 
GCTGGCGCAA TTTATCTAGA TAGCAATAAG GATTTGAAGA CTGTTTGGAG GGTGTTTTAC 
AAGATTATCT GGCGCGAAAT TGATCTTTTT AGCAAGAATG TGCCAAAAAA TGTCATCAGG 
AGATTGTACG AGTGCCATAC TGTTTACCCT CCGCAATTTA GTAAAGCGTT GGAAGTTGGT 
AATCAAAAGA CTATGGTTTC TTTAGACTTT ATGTGTGAAG GTAGAAAGAA ACGAGTTCAT 
GGGTTTGGAA CGAACAAAAT CTTGGCTAAA AGGGCCGCTG CCAAAATTGC TCTTCGTGCT 
TTAAAGTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium.
Genome biology9(1)R10(2008 Jan)
Tomoyasu Y,Miller SC,Tomita S,Schoppmeier M,Grossmann D,Bucher G

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Dcr-2 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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