LOC657327 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC657327
Entrez Gene ID	657327
Full Name	protein bark beetle
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008193559.2	XP_008191781.1	protein bark beetle isoform X1
XM_008193560.2	XP_008191782.1	protein bark beetle isoform X2
XM_008193561.2	XP_008191783.1	protein bark beetle isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC657327 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC657327 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTg04644	XM_008193559.2 Latest version!	Tribolium castaneum protein bark beetle (LOC657327), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTg04645	XM_008193560.2 Latest version!	Tribolium castaneum protein bark beetle (LOC657327), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTg04646	XM_008193561.2 Latest version!	Tribolium castaneum protein bark beetle (LOC657327), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC657327 ( XM_008193559.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008191781.1 Tribolium castaneum protein bark beetle (LOC657327), transcript variant X1, mRNA.

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CloneID	OTg04644
Clone ID Related Accession (Same CDS sequence)	XM_008193559.2
Accession Version	XM_008193559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	protein bark beetle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450161.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008193559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGTT TTTTGAGGAC GTTGCTTTTG GCCATCTGTG TTAGTAATGC CGTTCTGCAA 
GAAACGGAAG TGTTTTTTGG GAGTACGGAA GGGTCTAGGG GTGGCAGGAG TGAGCATCCT 
GGAGGGACGA TAGTTAACGA TAAGTTGGTT TTTTCGAAAT TTAAGAGTCC CTACTGGTTA 
AGGAATGACA TCATCGTTGA GAGAAACGCT GAATTAATTA TTGAACCAGG AGTAACCATC 
AACGTTGAAC CGCAGGTTGG AATTACCGTT CGAGGAGTTT TGACTGCTGT GGGTACCGAA 
AATGAACGAA TCGTTCTAAC GACCGCAGAG GAAACCTCTG CGAAGAATAT TAATTTCCCC 
GACATCCGGC TCTCTGACGG CCCCACGATC TTAGCGGGCC GTGTCCAAGT GCGTCACAAT 
GGCCGTTGGC GCAGCGTTTG CACCAACTCC AAAAATTGGA CGCGTGCCGA CATGGAAACG 
GCTTGCCGCC AACTCGGTTT CCAGGGCGGC TCGTTTTTCA ATTGGTTCGA CCGACAAATG 
CCCCTCAAAC CCCGACTTCT TTACGAAGAA CCCAAATGTT CAGGAACGGA AAGCTCTCTG 
TTCGACTGCG ACTGGAACTC GCGCCAAATG GGGGCCGGCG TGTGCGACTA TCACCCGGAC 
TTGGCCATCC AGTGTAGCCC CAGACACGAC AGAAATTTAC CGTTCTGGAG AGGCATCCGT 
TTTGAGGATG CTAAGCATAA TAAAGTCTTG ACACAGTCAA ACACTTTGTA TGTTCCTGTC 
TCGGTGTCGA AGCTACAATA CGTACAGATT TTATACGCGG GGGCCGGACG TGGCCAGAAC 
ACGTCCAGTG CTGTTGAAGT TGAGGGGATT CCCCCAGTTA TGGATAATCT AGAAATTATT 
AACTCGGCCT ACAATGGCAT TAACATAACC AGGCCGGAGG GACCCTTCAG TGTGACCAAC 
TGCACGCTGA GGGGTAACCG CGGTTATGGC ATTTTTGTCA ACTCCAGCCA TGGCTTAACC 
CATATCGATG GGTGCACTAT TAACGAAAAT GGGGGCGATG GGGTTAGATA CGTCCATGGG 
GAGGAACGTC CCGATGAAAG GAGCGATCGC TTCGGTTACG ACGATTTTTG TAACCTTGCG 
ATCGTTTCAA GTCAAACGTT CCCCTTGCAA CTTTTCGCCG AACAAACGCT GTTTCACAAC 
CAAATCAAAT CGTGCGATAA AGTCTTTTCG ACACGTAACC GCAACGTTAT CACCTTATCA 
GTCATTCGGG CTGTCACAAA TTTTAATAAC AGTGCCTCTC TGGAAGTCTA CGACGGCTCC 
ACTTTAAATC ACCGCTTACT TACCTCTTTC CCGATCAGAA ATAACACCAG ACCTCAGTCC 
ATTACAAGCA CTGGACAACA CCTTTTCGTA AAGTTTCGCG CAGCTGCGAG TACCGAAACC 
GTTGTCTTCA TGCGGGTTTT CGCAAGCCCC AGCAAAAGTT ACGACCTAAA TGTTAGTAAT 
TCGGACGTTT CCGAAAACAT GGGGCGCGGG ATCGCCGTTG ATAATTTGCG GTCACAAGTC 
CACGTTTACA AATCTTCGGT TTCGAAAAAC CAACATGTGG CTGGTCTTCA TGTTACGAGC 
GGCGTTGGCG ATGTTAACGT TACCGAAAGT CGCATTTCGT TCAATGAGGG TGATGGGATC 
AACATCACTT ATACTGGCGG TAGCAGGAAC GTCTCCAAGT CGTCAATTAG TTCAAACACT 
GGCTATGGAC TAGCCATTTG GCTGAACGAC ACCAAAGAAA CCGAATTTGT CACAGTGAAC 
CAAACCACAG TCGTCCAGTA TTCAGAGATT TACAAGAATC TGGACACGGG GATTTTACAC 
GGCAATTTCT GCGGCGAGTC TTTTTTTAAC TTTACGGGAA ACCGCTTCCG GAATTGTGCA 
AATGACGCGC TGGAAGTCCT CACCTGTTGG CGGAACACCA ACACTTCGAC GAGACTCCAA 
ATTGGCCATA ACGAATTTAT TGGTAATAAC AGGATCGGGT TGAGGCTAAC CCCAGCTTTG 
AACCTTGAGG CCACAATCGA ATACAACCAC TTCCGTCAAG GGTTATTCGG CGCTCTTTTA 
ATCAAAAACA AGCCTTTGGA GGAGTTTAAT GTCTTGAAAA CCAATATTCA AGTCTTCCAA 
AACTATTTTA TCAATAATAC GGGCACGTTT GTGGCCAACT TGGCCCTGTC ACCTTATGCC 
GAAAACCAAA ACTTGTTGTT TATCCGCAAC TACGTTAAAA ACAACCGGAT CTCGGAACCC 
TTCCAGCCGG AAGACGGCTC AATCTCTAGT CTGAACCCCC GGAGTCGGGT CGCTGCCCCT 
ATAGTAGTGG GGTCGGACAA TGTCGATATT TACCGGAACA TTATTGAAAA CCCTGACTCG 
AAATACGAAA TTGGGAGCCA CCTGGAGGAC CAAAGCAAGT CGCTTAACTG CACGTTTAAT 
TGGCTAGGGT TCGGGCACGA TGAGGAGATT CTACCGCGAC TTTTCCACCG TCTTGACCGT 
TATAATTTGG CCAAAATTGA GTTCACTCCA TTTTTATTGC ACAATACTAA TCCTCTGACC 
AACCGAATTA ACCAATACCA GTACTACATC CCGAAGTTTG TGTCCCCCAG CTCGAATAAA 
GTCGGGGGTG AAGTTGAAGG TGAGGAGACT TTGACTCCCG GTGAATATGT CGTGGAGAAA 
GACATTAATA TACGACCTGG GGGTAAATTG TCAATTGAAC CGGGGGTCAT TCTTAGGTTC 
CCACCTTCGG TGGGAATGAT GGTTGGCGGG AAACTTGAAG CTAGGGGTAT AGGCCCTGAT 
AGTATTAAAC TCACACTGAA GGAAGAGATT TTGCATGTGC CTGATAACGA AACGTACGAA 
ATTGAAACGG AAAAATACGA AGTCGAGACT GAAGTTGTGC AAATCGAGCC TAGAGTCCCG 
ATCCGACTGC TTGGGGGAGC GACGGAGACC GAAGGCCGTC TCCAGGTCAG AATCAACAAC 
GAGTGGGGCA CTGTCTGCAA CTATGGCTGG ACCATGACAA ACGCGGCCCT CGTGTGCCAA 
CAGCTGGGCT TTGTCTTGAA CCCCGAAGAC TGGTTCTTGA AACCTAGCGA GACCCCAGCC 
GCTGGCACGA GTGAAAATAT AATCTTGTCC AACGTCCAGT GTGACGATTT TGACTTGGAC 
GTCACCAAAT GTAAAGCCGA GACCAAAGGC AATTTCGAGA ACGCCTGCAC CCACCAGAAC 
GACGTGGGGA TCAGGTGCTA CAAATCGTCC TGGGCTGGGG TCCGCTTTGG GGCCCTGGCC 
GAACGAAGCA ACATCCAGTA TATCACAGTG GAAATGGCAG GACTCCTCGA TTACGCCACT 
CGTACCTTCA AGCCCGCAGT CCAGATTGAC TTTGCGAGGC AAAATTTCGA AAACATCCGA 
GTTTCCAATA ATTACTACCA CGGGTTGGGC GTCCTCTACG CCGATATTTA CACCGATGAT 
ACAATCAACA TAATTAAAAA CTCGCAGTTT TTGAACAATA AAGGCGCCGG TGTTAGTTTC 
AAGCAACTAG GGTTCAACAT TTTCGGTAGT TTGATTGAGA ACAATTACTT CGGTGTTAAG 
CACAATCCCG TCCTGACCGG GCTCCAGCAA CGCGAGTTCG GGGGTTGGTT CCTCACAAGT 
GACGATAACG TGCGGTACAA ACCGCTAATG ATCCCCGAAA CTTTGGAGGT GAACACCATA 
CAAGTCGACC GCGGGGAAAC GAAGTACTTG GTCACGTCCA AAGTCATTGG GGATGATATC 
TCGCGGGTTT ACCGCTTCCG GAGCGACCCA GGCTGGGTGA TTGGCATGCA ACTTCTGAAC 
CCGATTGAGA ATCGGAGCAC TGAAAGTATC GTCATTCAAG ACGCACTTTC GTACAACAAA 
AACGCCATTT ATTGGGTGCT TAAGCGCGAC CTGACTGTCT TCCCGACAAC GTCGAGTAGT 
TACGGGATTA TTCTGGAGTA CAATAGTGGG AGTTATGCCA TCGGTGGTAC TGTTATTGTG 
ATTAGTACGA TCAGGGCGCC GGTTCAGAAT ATACGCAACC GGATTGTCAA AGGCCCAGTC 
CCGACTCTAC GATTGGTTAA TTCCAAAATC AGGAGTAACC AATTTGGAAT ACACGCCTCG 
TACTATAACA GATATTTGAA CGAGCTGGGT GACCATTTTT TGCGCAAAGC TAACGAAAGT 
TTCCAGCTGG TCAATTGTGA GGTTTCGCAC AACTTGAACG AAGCGATTTT TGTGCATTCG 
CCCCATTGGG ACTTGCATAA GAGTAACCTT TCGGAGGTTG TTATCATGAT TAATAATTCA 
TTGATTACGG ACAATGGCAA GGGTTTTTAC CACTTTTCGC GCGACATGCG CGCCTCTAAC 
AACATTTTCC ATTACATTTT GCAAGACGAT ACAATTGAGA GAAACCGCGC TGGTGGGTTC 
GATGTCAATC TGCCGTACGT TTGGCAGTAC AATGAAAACT TCACCCATTC GGTTTACATG 
GATAACAACA CCTGGACGCA CAACCGTAAC TTCAAATTCA ACATTGACGG CCACTTCGCC 
GTGGTTAACG TCACCCGTAA CGTCTTCACC GATAATAATT GTGCCACCGG TTTGATCACA 
ATCGGCGGAA TGGAGAAAAA ACTATTGATA AGCCGTAACA AGTTCAATAC GAACAACGGC 
AAATTCATCG TAGAGTTTGT CTCAAACAGC CAGTCGGAGA TAATCGGCGA AGTCCCAGCC 
CAGTTCACTT TCAACGAACT CCGCCACAAC AAGTTTGCGT TCGGGGGCCG CAGCTTCGGC 
CTCTTGCAAC TCAAGAAAGA CCCCACGTCG GTGGTCGCCT TCAAAGGCAT CCAAAAAGTC 
AAAATTTTCC GAAACCTATT TTCGCAAAAC CAGCTCGATT ATCAACTGAT CGCGGGGATC 
AAAACCGCCA AAATCGACAC TTTTGTGGAC GTGCGCGAAA ATTGGTGGGG CACGAACGAC 
GAGGTGGAGA TCAAGGAGAA GATTTTCGAT TTCGACGACT GGAACAACTA TGCCATTGCC 
AAGTTCAAGC CCTACTTGGT CAATGACGAT TTCGCGTCGT CTTATTCCGC TGGTTATATT 
AGTGCCACCA CCACTGATTT GAACAAACTT GGGGGGCGGA TCACCCAGGA CTTGGTCTTG 
CATAATGAGA GAAATGCTCC GTATGTCGTC CAGTCCGACA TAACCGTTAT GCCAAAAGTC 
ACCCTGACAA TTGACCCGGG GGTGGTTCTG GAGTTTGCGC CAAACGTTGG TATCCTGGTC 
TTGGGGAGTT TGCAAGCGCG GGGTTACGAA GGCAACGAGA TTATCATGCG GCCGATTTCC 
AAAACGGGTT ATTTGGAGTC GAGTTCGACA GTTCGGGAAA AACGCCAAAT TGAGAATTTG 
GTCGGTGGCG AAAGCATCCG TTTGTGCAAG GGCCGGGGGT GTTACGACGG CGAGGAGAGC 
ACCCCACGGG AGGGTTTCCT CGAATATTTC AATAAAACCA CCCTCCAGTG GATCCCCATG 
TGCGATACAA GGTTTACGGA GCGGAACGCG CAAGTTGTGT GCCGGGAGTT GGGTTTTGAC 
CCACTTAACG TGTTTTTCGA CCATGATGTC AGGATCGAGT TTCATAGTAA CTCGCTTTCG 
CGGATTTGGA CCTGGCCTGA GCCGCTCCAG TGCAAAGGAA CGGAGAAAAA ATACGAGGAC 
TGTCCCATCA GACTGAATGG GCAACAGTTC GGACACCGCC ACCGGTGCAA GTGGAACTCT 
AAGTTTGTGT TCATTCACTG TGGGCGAAGG AATTTGGAGC CGAAGTATGA TTATTGGGGC 
GGTGTTAGGT TTGCCAATCC TGAGTTTGAG CAACAGTTGT ACGCTCATCG CATTCACGAC 
GTGAAAACAC ACTCGACCAT GCAGAGTCAG GAGTCGGTGA TGGAGTATGT TAATATAATC 
GGGGCTGGGA TTTTGCATAA TGAGAAATCG CCGGCGATTC AGAGCATTAT TAAGAGCCCC 
AAGATTAATT TCGTGAGGGT TTACAAAAGT GCCTCACATG GGATTAATTT GATTTCGCCT 
TCGAACACAA TGAATCTGTT GTATAACACG GTCGAGGATT CGCTGGGTGT GGGCATAAAT 
ACGTTGTCAG TGACCGGCGA AGGGAGAGAA TCGCAAGAAT CAAGCTTCAC TCCCTTGAAA 
AGTTTGAACA TTCCCTACAA TCTGTTCAGT CTGGTTGACA TGTGCGACAC CCACAAGGAG 
ATAGAGATCC AGGAGCGCAT CCTGCTGTAC TACAAGTACG ACAATCACCC AGTCAATTGC 
ATCAAAATTT TCAAGAGCGC CTACAACATC AAACCGCTCG GCTTCCGTTT GCTACAGTTC 
AACTTGTTTA ACTCGACCCA AAAATACGGA ATCCCCGATT TTATCTCACT GTACGACGGT 
GATATTTACA ACGTCACCTC GAAACTTATC GACCGCATAA CCATGATGTC CCACAACGAG 
AAAAGACTAG TCCGAACCAA ATTGCCAAGC CTTAGTATCA AACTTTTCGC AAATGGAGCT 
TCGTCCAATC ACGGTTTTAT CGCCGAAGTT GTCACACTTC CAATCTCAGC CATCGGCTTC 
AATCGGGACG TGCAACACAA CATTTCTTAT TCCGTGGTGA ACAACAACAG GGAAGGCGCA 
ATGTTGTACA TGTCAGCGGG CGAAGTCAAC CCGATTGTGA CCATTGACAA GAACCAGTTT 
AGATACAATT GTGAGAAGCT GTACGGGAAT TTCACGACTT GTGATGCCGC CGTGGAGATC 
GATATACAAA ACACCCAGAC TATTTTTTTC AGAAATAATT TGGTTGAAGG TAACCAAGGG 
GGGTTGGCGA TCAAGGCGGA TTCGCGCGGC TCAGCCACCT CTCTCAAGGG TTACATCCAC 
AATAATTTGT TTGTGAATAA TAGCAACTTG CCGGTGTTGT ACGTGGAAGG ACGGCAGTCG 
TCCCCCTATC AAGAAGTGGT GATTTACCGA AACTACTTTA CGAGGAACAA AGCACAATAC 
TACAATAATA TTGTTCTGAA ACAGGTCGTT TCCAACTTTA CGCTAAATTA CGTGAAGAGG 
AATGTCGGTT TGAAAAATTT GGAAGTGTCA GGGTTTGATA AAGTCAGGCT TCCGATTTAC 
CAAACGACGT CTCATAATGG GTTTTATCAT AATTATGCCA TTCATCGCGA TAGCAGATCG 
ACGATTGTGG CGGGGACTGC AGGTCAACAT TACGTGGATA ATATCTTCTT TAACCCAGAT 
AATGATTATG AAATGATAAC AGTAAACAGA TCTCTATTTG AATTCAACAG CACTTTACAA 
TTATGGAACA CAAAAATCGA CGCTGCTTAC AACTACTGGG GCCTTAACAA CTCTCTGGGT 
GTCCGAGGTC GCATCAAGGA CCAAAGCGAC GATCCTCGTC TCCTTGAAGT TATTTACGAG 
CCTTATCACA TGAACAATAA ATCAGTTTTA CTCAATAAAA AATGTCCCCC GGGATGGGAT 
CTCGTCGGAG GGACTTGTTA TATTTATATT GGTGCTCCCA TGACCTTCCA TGAAGCTCGA 
GCTTTTTGTC AGGCTGATAA CGCAACAATG CCGTATTTGT TAGGCAATTT GAATTACATC 
GAACTGTATG AGTTTTTACG ATCGCAACAA CAGTGGTTTT TGTACAATGA TAGGATTTGG 
GTGCAACATA TCGACAAAAT CAACGAGTGC ACCATGTTTG CCTACCAAAG GGTCGAGGTT 
GATAACTGCG ATCAAAGGAG TCCCTTCATC TGCGAAATAG ATCCTAAAGT TTCGATAAAC 
ATCCTGCCAT TGACCGACGA TGTTGTTACA ATAGCCGTTC TGTCAAGCCT GGCTTTGGCC 
TTACTTTTGA TGGCCATAGT CGGTGTTTTT TGGTGGAGCA AATCAAAGTA TCGCCAAACC 
CAAAGGCTGG AAAGACGGAA CAGTATCAGG CAAAGTTTAC ATTCATTGCG CTCCGTTGGC 
CTGGCACCTG GCAGTTTCTC TGACGCTAGT TACCGCCGAA AAGTGGCACA ACTTAGCTCG 
CGCTCAACCG ACACACTAAC AAAAAATTCC GAATACAAGA AAATGATAAC GAATGGATCA 
CTAGACAGTA TGGAAAAATC CACCTACACG TCGTCCCTTG ACGACAACCA GTCGTATGAC 
GTTTACGAGG CCCATAACCC TAACCCTTCC GCTTTTGCTT ATAGCCCTAC AATTGAGTAT 
CACAAATCAC CAAATAGGTT CGATGCCCAG TATGCCAAAC CTAACGGGTT CGAACTGGCG 
TACAAAAACG AGGGTTTTCG CGATAATTCG ACTTTTGCAA CGAATTCAAA TTATCAATCA 
CGGGCCGAAA GCATCCAAGA CAGTACCAAC GACGACATCC CGATTATCCA AGACAACGTT 
GGCGGGAACA GTGTTTCCTA CCCCCCTAGT GAATACTACA CCACTGACAC GCTCCCCCTG 
AGCGGAAAAT CGGACTCAAC CCTTGACCTG AAGAAGGGAA TTGATGATTA TAATAAAAAC 
CGCAACTACG ACCCGATCTA CAGTCGCGAA AGACCAAATG CGAATTTGTT GAACGAGTTG 
AAAAATAAAT TCCCGCAAAC GCCGCCGCCG CCACCAGACC CGGTCCAATC GCCCACTTAT 
TCGGAAAAGT TAGACAGTCT GCAATACTCC CCTGATTATA ACACGGTGGC GTTAGCTGAG 
CCCTTCTCTG CCAATATACT TGAGACCGAC TTTGACGAGC CGCACCCACG GCCGAAAGTT 
CGGGCCAAAA GTGAGGTGCT GCTAGAGACT AACTTTGACT ACACTCCGCC TAGTGGTAGT 
CCGCAGTTGA ATCACCCAAT CACCGAATAT AATAGAAGTA AGAGTCAACC GTTGGAGACA 
GCGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008191781.1
CDS	405..9293
Translation	MCSFLRTLLLAICVSNAVLQETEVFFGSTEGSRGGRSEHPGGTIVNDKLVFSKFKSPYWLRNDIIVERNAELIIEPGVTINVEPQVGITVRGVLTAVGTENERIVLTTAEETSAKNINFPDIRLSDGPTILAGRVQVRHNGRWRSVCTNSKNWTRADMETACRQLGFQGGSFFNWFDRQMPLKPRLLYEEPKCSGTESSLFDCDWNSRQMGAGVCDYHPDLAIQCSPRHDRNLPFWRGIRFEDAKHNKVLTQSNTLYVPVSVSKLQYVQILYAGAGRGQNTSSAVEVEGIPPVMDNLEIINSAYNGINITRPEGPFSVTNCTLRGNRGYGIFVNSSHGLTHIDGCTINENGGDGVRYVHGEERPDERSDRFGYDDFCNLAIVSSQTFPLQLFAEQTLFHNQIKSCDKVFSTRNRNVITLSVIRAVTNFNNSASLEVYDGSTLNHRLLTSFPIRNNTRPQSITSTGQHLFVKFRAAASTETVVFMRVFASPSKSYDLNVSNSDVSENMGRGIAVDNLRSQVHVYKSSVSKNQHVAGLHVTSGVGDVNVTESRISFNEGDGINITYTGGSRNVSKSSISSNTGYGLAIWLNDTKETEFVTVNQTTVVQYSEIYKNLDTGILHGNFCGESFFNFTGNRFRNCANDALEVLTCWRNTNTSTRLQIGHNEFIGNNRIGLRLTPALNLEATIEYNHFRQGLFGALLIKNKPLEEFNVLKTNIQVFQNYFINNTGTFVANLALSPYAENQNLLFIRNYVKNNRISEPFQPEDGSISSLNPRSRVAAPIVVGSDNVDIYRNIIENPDSKYEIGSHLEDQSKSLNCTFNWLGFGHDEEILPRLFHRLDRYNLAKIEFTPFLLHNTNPLTNRINQYQYYIPKFVSPSSNKVGGEVEGEETLTPGEYVVEKDINIRPGGKLSIEPGVILRFPPSVGMMVGGKLEARGIGPDSIKLTLKEEILHVPDNETYEIETEKYEVETEVVQIEPRVPIRLLGGATETEGRLQVRINNEWGTVCNYGWTMTNAALVCQQLGFVLNPEDWFLKPSETPAAGTSENIILSNVQCDDFDLDVTKCKAETKGNFENACTHQNDVGIRCYKSSWAGVRFGALAERSNIQYITVEMAGLLDYATRTFKPAVQIDFARQNFENIRVSNNYYHGLGVLYADIYTDDTINIIKNSQFLNNKGAGVSFKQLGFNIFGSLIENNYFGVKHNPVLTGLQQREFGGWFLTSDDNVRYKPLMIPETLEVNTIQVDRGETKYLVTSKVIGDDISRVYRFRSDPGWVIGMQLLNPIENRSTESIVIQDALSYNKNAIYWVLKRDLTVFPTTSSSYGIILEYNSGSYAIGGTVIVISTIRAPVQNIRNRIVKGPVPTLRLVNSKIRSNQFGIHASYYNRYLNELGDHFLRKANESFQLVNCEVSHNLNEAIFVHSPHWDLHKSNLSEVVIMINNSLITDNGKGFYHFSRDMRASNNIFHYILQDDTIERNRAGGFDVNLPYVWQYNENFTHSVYMDNNTWTHNRNFKFNIDGHFAVVNVTRNVFTDNNCATGLITIGGMEKKLLISRNKFNTNNGKFIVEFVSNSQSEIIGEVPAQFTFNELRHNKFAFGGRSFGLLQLKKDPTSVVAFKGIQKVKIFRNLFSQNQLDYQLIAGIKTAKIDTFVDVRENWWGTNDEVEIKEKIFDFDDWNNYAIAKFKPYLVNDDFASSYSAGYISATTTDLNKLGGRITQDLVLHNERNAPYVVQSDITVMPKVTLTIDPGVVLEFAPNVGILVLGSLQARGYEGNEIIMRPISKTGYLESSSTVREKRQIENLVGGESIRLCKGRGCYDGEESTPREGFLEYFNKTTLQWIPMCDTRFTERNAQVVCRELGFDPLNVFFDHDVRIEFHSNSLSRIWTWPEPLQCKGTEKKYEDCPIRLNGQQFGHRHRCKWNSKFVFIHCGRRNLEPKYDYWGGVRFANPEFEQQLYAHRIHDVKTHSTMQSQESVMEYVNIIGAGILHNEKSPAIQSIIKSPKINFVRVYKSASHGINLISPSNTMNLLYNTVEDSLGVGINTLSVTGEGRESQESSFTPLKSLNIPYNLFSLVDMCDTHKEIEIQERILLYYKYDNHPVNCIKIFKSAYNIKPLGFRLLQFNLFNSTQKYGIPDFISLYDGDIYNVTSKLIDRITMMSHNEKRLVRTKLPSLSIKLFANGASSNHGFIAEVVTLPISAIGFNRDVQHNISYSVVNNNREGAMLYMSAGEVNPIVTIDKNQFRYNCEKLYGNFTTCDAAVEIDIQNTQTIFFRNNLVEGNQGGLAIKADSRGSATSLKGYIHNNLFVNNSNLPVLYVEGRQSSPYQEVVIYRNYFTRNKAQYYNNIVLKQVVSNFTLNYVKRNVGLKNLEVSGFDKVRLPIYQTTSHNGFYHNYAIHRDSRSTIVAGTAGQHYVDNIFFNPDNDYEMITVNRSLFEFNSTLQLWNTKIDAAYNYWGLNNSLGVRGRIKDQSDDPRLLEVIYEPYHMNNKSVLLNKKCPPGWDLVGGTCYIYIGAPMTFHEARAFCQADNATMPYLLGNLNYIELYEFLRSQQQWFLYNDRIWVQHIDKINECTMFAYQRVEVDNCDQRSPFICEIDPKVSINILPLTDDVVTIAVLSSLALALLLMAIVGVFWWSKSKYRQTQRLERRNSIRQSLHSLRSVGLAPGSFSDASYRRKVAQLSSRSTDTLTKNSEYKKMITNGSLDSMEKSTYTSSLDDNQSYDVYEAHNPNPSAFAYSPTIEYHKSPNRFDAQYAKPNGFELAYKNEGFRDNSTFATNSNYQSRAESIQDSTNDDIPIIQDNVGGNSVSYPPSEYYTTDTLPLSGKSDSTLDLKKGIDDYNKNRNYDPIYSRERPNANLLNELKNKFPQTPPPPPDPVQSPTYSEKLDSLQYSPDYNTVALAEPFSANILETDFDEPHPRPKVRAKSEVLLETNFDYTPPSGSPQLNHPITEYNRSKSQPLETAM

Target ORF information:

RefSeq Version	XM_008193559.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum protein bark beetle (LOC657327), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008193559.2

ORF Insert Sequence:

ATGTGTAGTT TTTTGAGGAC GTTGCTTTTG GCCATCTGTG TTAGTAATGC CGTTCTGCAA 
GAAACGGAAG TGTTTTTTGG GAGTACGGAA GGGTCTAGGG GTGGCAGGAG TGAGCATCCT 
GGAGGGACGA TAGTTAACGA TAAGTTGGTT TTTTCGAAAT TTAAGAGTCC CTACTGGTTA 
AGGAATGACA TCATCGTTGA GAGAAACGCT GAATTAATTA TTGAACCAGG AGTAACCATC 
AACGTTGAAC CGCAGGTTGG AATTACCGTT CGAGGAGTTT TGACTGCTGT GGGTACCGAA 
AATGAACGAA TCGTTCTAAC GACCGCAGAG GAAACCTCTG CGAAGAATAT TAATTTCCCC 
GACATCCGGC TCTCTGACGG CCCCACGATC TTAGCGGGCC GTGTCCAAGT GCGTCACAAT 
GGCCGTTGGC GCAGCGTTTG CACCAACTCC AAAAATTGGA CGCGTGCCGA CATGGAAACG 
GCTTGCCGCC AACTCGGTTT CCAGGGCGGC TCGTTTTTCA ATTGGTTCGA CCGACAAATG 
CCCCTCAAAC CCCGACTTCT TTACGAAGAA CCCAAATGTT CAGGAACGGA AAGCTCTCTG 
TTCGACTGCG ACTGGAACTC GCGCCAAATG GGGGCCGGCG TGTGCGACTA TCACCCGGAC 
TTGGCCATCC AGTGTAGCCC CAGACACGAC AGAAATTTAC CGTTCTGGAG AGGCATCCGT 
TTTGAGGATG CTAAGCATAA TAAAGTCTTG ACACAGTCAA ACACTTTGTA TGTTCCTGTC 
TCGGTGTCGA AGCTACAATA CGTACAGATT TTATACGCGG GGGCCGGACG TGGCCAGAAC 
ACGTCCAGTG CTGTTGAAGT TGAGGGGATT CCCCCAGTTA TGGATAATCT AGAAATTATT 
AACTCGGCCT ACAATGGCAT TAACATAACC AGGCCGGAGG GACCCTTCAG TGTGACCAAC 
TGCACGCTGA GGGGTAACCG CGGTTATGGC ATTTTTGTCA ACTCCAGCCA TGGCTTAACC 
CATATCGATG GGTGCACTAT TAACGAAAAT GGGGGCGATG GGGTTAGATA CGTCCATGGG 
GAGGAACGTC CCGATGAAAG GAGCGATCGC TTCGGTTACG ACGATTTTTG TAACCTTGCG 
ATCGTTTCAA GTCAAACGTT CCCCTTGCAA CTTTTCGCCG AACAAACGCT GTTTCACAAC 
CAAATCAAAT CGTGCGATAA AGTCTTTTCG ACACGTAACC GCAACGTTAT CACCTTATCA 
GTCATTCGGG CTGTCACAAA TTTTAATAAC AGTGCCTCTC TGGAAGTCTA CGACGGCTCC 
ACTTTAAATC ACCGCTTACT TACCTCTTTC CCGATCAGAA ATAACACCAG ACCTCAGTCC 
ATTACAAGCA CTGGACAACA CCTTTTCGTA AAGTTTCGCG CAGCTGCGAG TACCGAAACC 
GTTGTCTTCA TGCGGGTTTT CGCAAGCCCC AGCAAAAGTT ACGACCTAAA TGTTAGTAAT 
TCGGACGTTT CCGAAAACAT GGGGCGCGGG ATCGCCGTTG ATAATTTGCG GTCACAAGTC 
CACGTTTACA AATCTTCGGT TTCGAAAAAC CAACATGTGG CTGGTCTTCA TGTTACGAGC 
GGCGTTGGCG ATGTTAACGT TACCGAAAGT CGCATTTCGT TCAATGAGGG TGATGGGATC 
AACATCACTT ATACTGGCGG TAGCAGGAAC GTCTCCAAGT CGTCAATTAG TTCAAACACT 
GGCTATGGAC TAGCCATTTG GCTGAACGAC ACCAAAGAAA CCGAATTTGT CACAGTGAAC 
CAAACCACAG TCGTCCAGTA TTCAGAGATT TACAAGAATC TGGACACGGG GATTTTACAC 
GGCAATTTCT GCGGCGAGTC TTTTTTTAAC TTTACGGGAA ACCGCTTCCG GAATTGTGCA 
AATGACGCGC TGGAAGTCCT CACCTGTTGG CGGAACACCA ACACTTCGAC GAGACTCCAA 
ATTGGCCATA ACGAATTTAT TGGTAATAAC AGGATCGGGT TGAGGCTAAC CCCAGCTTTG 
AACCTTGAGG CCACAATCGA ATACAACCAC TTCCGTCAAG GGTTATTCGG CGCTCTTTTA 
ATCAAAAACA AGCCTTTGGA GGAGTTTAAT GTCTTGAAAA CCAATATTCA AGTCTTCCAA 
AACTATTTTA TCAATAATAC GGGCACGTTT GTGGCCAACT TGGCCCTGTC ACCTTATGCC 
GAAAACCAAA ACTTGTTGTT TATCCGCAAC TACGTTAAAA ACAACCGGAT CTCGGAACCC 
TTCCAGCCGG AAGACGGCTC AATCTCTAGT CTGAACCCCC GGAGTCGGGT CGCTGCCCCT 
ATAGTAGTGG GGTCGGACAA TGTCGATATT TACCGGAACA TTATTGAAAA CCCTGACTCG 
AAATACGAAA TTGGGAGCCA CCTGGAGGAC CAAAGCAAGT CGCTTAACTG CACGTTTAAT 
TGGCTAGGGT TCGGGCACGA TGAGGAGATT CTACCGCGAC TTTTCCACCG TCTTGACCGT 
TATAATTTGG CCAAAATTGA GTTCACTCCA TTTTTATTGC ACAATACTAA TCCTCTGACC 
AACCGAATTA ACCAATACCA GTACTACATC CCGAAGTTTG TGTCCCCCAG CTCGAATAAA 
GTCGGGGGTG AAGTTGAAGG TGAGGAGACT TTGACTCCCG GTGAATATGT CGTGGAGAAA 
GACATTAATA TACGACCTGG GGGTAAATTG TCAATTGAAC CGGGGGTCAT TCTTAGGTTC 
CCACCTTCGG TGGGAATGAT GGTTGGCGGG AAACTTGAAG CTAGGGGTAT AGGCCCTGAT 
AGTATTAAAC TCACACTGAA GGAAGAGATT TTGCATGTGC CTGATAACGA AACGTACGAA 
ATTGAAACGG AAAAATACGA AGTCGAGACT GAAGTTGTGC AAATCGAGCC TAGAGTCCCG 
ATCCGACTGC TTGGGGGAGC GACGGAGACC GAAGGCCGTC TCCAGGTCAG AATCAACAAC 
GAGTGGGGCA CTGTCTGCAA CTATGGCTGG ACCATGACAA ACGCGGCCCT CGTGTGCCAA 
CAGCTGGGCT TTGTCTTGAA CCCCGAAGAC TGGTTCTTGA AACCTAGCGA GACCCCAGCC 
GCTGGCACGA GTGAAAATAT AATCTTGTCC AACGTCCAGT GTGACGATTT TGACTTGGAC 
GTCACCAAAT GTAAAGCCGA GACCAAAGGC AATTTCGAGA ACGCCTGCAC CCACCAGAAC 
GACGTGGGGA TCAGGTGCTA CAAATCGTCC TGGGCTGGGG TCCGCTTTGG GGCCCTGGCC 
GAACGAAGCA ACATCCAGTA TATCACAGTG GAAATGGCAG GACTCCTCGA TTACGCCACT 
CGTACCTTCA AGCCCGCAGT CCAGATTGAC TTTGCGAGGC AAAATTTCGA AAACATCCGA 
GTTTCCAATA ATTACTACCA CGGGTTGGGC GTCCTCTACG CCGATATTTA CACCGATGAT 
ACAATCAACA TAATTAAAAA CTCGCAGTTT TTGAACAATA AAGGCGCCGG TGTTAGTTTC 
AAGCAACTAG GGTTCAACAT TTTCGGTAGT TTGATTGAGA ACAATTACTT CGGTGTTAAG 
CACAATCCCG TCCTGACCGG GCTCCAGCAA CGCGAGTTCG GGGGTTGGTT CCTCACAAGT 
GACGATAACG TGCGGTACAA ACCGCTAATG ATCCCCGAAA CTTTGGAGGT GAACACCATA 
CAAGTCGACC GCGGGGAAAC GAAGTACTTG GTCACGTCCA AAGTCATTGG GGATGATATC 
TCGCGGGTTT ACCGCTTCCG GAGCGACCCA GGCTGGGTGA TTGGCATGCA ACTTCTGAAC 
CCGATTGAGA ATCGGAGCAC TGAAAGTATC GTCATTCAAG ACGCACTTTC GTACAACAAA 
AACGCCATTT ATTGGGTGCT TAAGCGCGAC CTGACTGTCT TCCCGACAAC GTCGAGTAGT 
TACGGGATTA TTCTGGAGTA CAATAGTGGG AGTTATGCCA TCGGTGGTAC TGTTATTGTG 
ATTAGTACGA TCAGGGCGCC GGTTCAGAAT ATACGCAACC GGATTGTCAA AGGCCCAGTC 
CCGACTCTAC GATTGGTTAA TTCCAAAATC AGGAGTAACC AATTTGGAAT ACACGCCTCG 
TACTATAACA GATATTTGAA CGAGCTGGGT GACCATTTTT TGCGCAAAGC TAACGAAAGT 
TTCCAGCTGG TCAATTGTGA GGTTTCGCAC AACTTGAACG AAGCGATTTT TGTGCATTCG 
CCCCATTGGG ACTTGCATAA GAGTAACCTT TCGGAGGTTG TTATCATGAT TAATAATTCA 
TTGATTACGG ACAATGGCAA GGGTTTTTAC CACTTTTCGC GCGACATGCG CGCCTCTAAC 
AACATTTTCC ATTACATTTT GCAAGACGAT ACAATTGAGA GAAACCGCGC TGGTGGGTTC 
GATGTCAATC TGCCGTACGT TTGGCAGTAC AATGAAAACT TCACCCATTC GGTTTACATG 
GATAACAACA CCTGGACGCA CAACCGTAAC TTCAAATTCA ACATTGACGG CCACTTCGCC 
GTGGTTAACG TCACCCGTAA CGTCTTCACC GATAATAATT GTGCCACCGG TTTGATCACA 
ATCGGCGGAA TGGAGAAAAA ACTATTGATA AGCCGTAACA AGTTCAATAC GAACAACGGC 
AAATTCATCG TAGAGTTTGT CTCAAACAGC CAGTCGGAGA TAATCGGCGA AGTCCCAGCC 
CAGTTCACTT TCAACGAACT CCGCCACAAC AAGTTTGCGT TCGGGGGCCG CAGCTTCGGC 
CTCTTGCAAC TCAAGAAAGA CCCCACGTCG GTGGTCGCCT TCAAAGGCAT CCAAAAAGTC 
AAAATTTTCC GAAACCTATT TTCGCAAAAC CAGCTCGATT ATCAACTGAT CGCGGGGATC 
AAAACCGCCA AAATCGACAC TTTTGTGGAC GTGCGCGAAA ATTGGTGGGG CACGAACGAC 
GAGGTGGAGA TCAAGGAGAA GATTTTCGAT TTCGACGACT GGAACAACTA TGCCATTGCC 
AAGTTCAAGC CCTACTTGGT CAATGACGAT TTCGCGTCGT CTTATTCCGC TGGTTATATT 
AGTGCCACCA CCACTGATTT GAACAAACTT GGGGGGCGGA TCACCCAGGA CTTGGTCTTG 
CATAATGAGA GAAATGCTCC GTATGTCGTC CAGTCCGACA TAACCGTTAT GCCAAAAGTC 
ACCCTGACAA TTGACCCGGG GGTGGTTCTG GAGTTTGCGC CAAACGTTGG TATCCTGGTC 
TTGGGGAGTT TGCAAGCGCG GGGTTACGAA GGCAACGAGA TTATCATGCG GCCGATTTCC 
AAAACGGGTT ATTTGGAGTC GAGTTCGACA GTTCGGGAAA AACGCCAAAT TGAGAATTTG 
GTCGGTGGCG AAAGCATCCG TTTGTGCAAG GGCCGGGGGT GTTACGACGG CGAGGAGAGC 
ACCCCACGGG AGGGTTTCCT CGAATATTTC AATAAAACCA CCCTCCAGTG GATCCCCATG 
TGCGATACAA GGTTTACGGA GCGGAACGCG CAAGTTGTGT GCCGGGAGTT GGGTTTTGAC 
CCACTTAACG TGTTTTTCGA CCATGATGTC AGGATCGAGT TTCATAGTAA CTCGCTTTCG 
CGGATTTGGA CCTGGCCTGA GCCGCTCCAG TGCAAAGGAA CGGAGAAAAA ATACGAGGAC 
TGTCCCATCA GACTGAATGG GCAACAGTTC GGACACCGCC ACCGGTGCAA GTGGAACTCT 
AAGTTTGTGT TCATTCACTG TGGGCGAAGG AATTTGGAGC CGAAGTATGA TTATTGGGGC 
GGTGTTAGGT TTGCCAATCC TGAGTTTGAG CAACAGTTGT ACGCTCATCG CATTCACGAC 
GTGAAAACAC ACTCGACCAT GCAGAGTCAG GAGTCGGTGA TGGAGTATGT TAATATAATC 
GGGGCTGGGA TTTTGCATAA TGAGAAATCG CCGGCGATTC AGAGCATTAT TAAGAGCCCC 
AAGATTAATT TCGTGAGGGT TTACAAAAGT GCCTCACATG GGATTAATTT GATTTCGCCT 
TCGAACACAA TGAATCTGTT GTATAACACG GTCGAGGATT CGCTGGGTGT GGGCATAAAT 
ACGTTGTCAG TGACCGGCGA AGGGAGAGAA TCGCAAGAAT CAAGCTTCAC TCCCTTGAAA 
AGTTTGAACA TTCCCTACAA TCTGTTCAGT CTGGTTGACA TGTGCGACAC CCACAAGGAG 
ATAGAGATCC AGGAGCGCAT CCTGCTGTAC TACAAGTACG ACAATCACCC AGTCAATTGC 
ATCAAAATTT TCAAGAGCGC CTACAACATC AAACCGCTCG GCTTCCGTTT GCTACAGTTC 
AACTTGTTTA ACTCGACCCA AAAATACGGA ATCCCCGATT TTATCTCACT GTACGACGGT 
GATATTTACA ACGTCACCTC GAAACTTATC GACCGCATAA CCATGATGTC CCACAACGAG 
AAAAGACTAG TCCGAACCAA ATTGCCAAGC CTTAGTATCA AACTTTTCGC AAATGGAGCT 
TCGTCCAATC ACGGTTTTAT CGCCGAAGTT GTCACACTTC CAATCTCAGC CATCGGCTTC 
AATCGGGACG TGCAACACAA CATTTCTTAT TCCGTGGTGA ACAACAACAG GGAAGGCGCA 
ATGTTGTACA TGTCAGCGGG CGAAGTCAAC CCGATTGTGA CCATTGACAA GAACCAGTTT 
AGATACAATT GTGAGAAGCT GTACGGGAAT TTCACGACTT GTGATGCCGC CGTGGAGATC 
GATATACAAA ACACCCAGAC TATTTTTTTC AGAAATAATT TGGTTGAAGG TAACCAAGGG 
GGGTTGGCGA TCAAGGCGGA TTCGCGCGGC TCAGCCACCT CTCTCAAGGG TTACATCCAC 
AATAATTTGT TTGTGAATAA TAGCAACTTG CCGGTGTTGT ACGTGGAAGG ACGGCAGTCG 
TCCCCCTATC AAGAAGTGGT GATTTACCGA AACTACTTTA CGAGGAACAA AGCACAATAC 
TACAATAATA TTGTTCTGAA ACAGGTCGTT TCCAACTTTA CGCTAAATTA CGTGAAGAGG 
AATGTCGGTT TGAAAAATTT GGAAGTGTCA GGGTTTGATA AAGTCAGGCT TCCGATTTAC 
CAAACGACGT CTCATAATGG GTTTTATCAT AATTATGCCA TTCATCGCGA TAGCAGATCG 
ACGATTGTGG CGGGGACTGC AGGTCAACAT TACGTGGATA ATATCTTCTT TAACCCAGAT 
AATGATTATG AAATGATAAC AGTAAACAGA TCTCTATTTG AATTCAACAG CACTTTACAA 
TTATGGAACA CAAAAATCGA CGCTGCTTAC AACTACTGGG GCCTTAACAA CTCTCTGGGT 
GTCCGAGGTC GCATCAAGGA CCAAAGCGAC GATCCTCGTC TCCTTGAAGT TATTTACGAG 
CCTTATCACA TGAACAATAA ATCAGTTTTA CTCAATAAAA AATGTCCCCC GGGATGGGAT 
CTCGTCGGAG GGACTTGTTA TATTTATATT GGTGCTCCCA TGACCTTCCA TGAAGCTCGA 
GCTTTTTGTC AGGCTGATAA CGCAACAATG CCGTATTTGT TAGGCAATTT GAATTACATC 
GAACTGTATG AGTTTTTACG ATCGCAACAA CAGTGGTTTT TGTACAATGA TAGGATTTGG 
GTGCAACATA TCGACAAAAT CAACGAGTGC ACCATGTTTG CCTACCAAAG GGTCGAGGTT 
GATAACTGCG ATCAAAGGAG TCCCTTCATC TGCGAAATAG ATCCTAAAGT TTCGATAAAC 
ATCCTGCCAT TGACCGACGA TGTTGTTACA ATAGCCGTTC TGTCAAGCCT GGCTTTGGCC 
TTACTTTTGA TGGCCATAGT CGGTGTTTTT TGGTGGAGCA AATCAAAGTA TCGCCAAACC 
CAAAGGCTGG AAAGACGGAA CAGTATCAGG CAAAGTTTAC ATTCATTGCG CTCCGTTGGC 
CTGGCACCTG GCAGTTTCTC TGACGCTAGT TACCGCCGAA AAGTGGCACA ACTTAGCTCG 
CGCTCAACCG ACACACTAAC AAAAAATTCC GAATACAAGA AAATGATAAC GAATGGATCA 
CTAGACAGTA TGGAAAAATC CACCTACACG TCGTCCCTTG ACGACAACCA GTCGTATGAC 
GTTTACGAGG CCCATAACCC TAACCCTTCC GCTTTTGCTT ATAGCCCTAC AATTGAGTAT 
CACAAATCAC CAAATAGGTT CGATGCCCAG TATGCCAAAC CTAACGGGTT CGAACTGGCG 
TACAAAAACG AGGGTTTTCG CGATAATTCG ACTTTTGCAA CGAATTCAAA TTATCAATCA 
CGGGCCGAAA GCATCCAAGA CAGTACCAAC GACGACATCC CGATTATCCA AGACAACGTT 
GGCGGGAACA GTGTTTCCTA CCCCCCTAGT GAATACTACA CCACTGACAC GCTCCCCCTG 
AGCGGAAAAT CGGACTCAAC CCTTGACCTG AAGAAGGGAA TTGATGATTA TAATAAAAAC 
CGCAACTACG ACCCGATCTA CAGTCGCGAA AGACCAAATG CGAATTTGTT GAACGAGTTG 
AAAAATAAAT TCCCGCAAAC GCCGCCGCCG CCACCAGACC CGGTCCAATC GCCCACTTAT 
TCGGAAAAGT TAGACAGTCT GCAATACTCC CCTGATTATA ACACGGTGGC GTTAGCTGAG 
CCCTTCTCTG CCAATATACT TGAGACCGAC TTTGACGAGC CGCACCCACG GCCGAAAGTT 
CGGGCCAAAA GTGAGGTGCT GCTAGAGACT AACTTTGACT ACACTCCGCC TAGTGGTAGT 
CCGCAGTTGA ATCACCCAAT CACCGAATAT AATAGAAGTA AGAGTCAACC GTTGGAGACA 
GCGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC657327 ( XM_008193560.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008191782.1 Tribolium castaneum protein bark beetle (LOC657327), transcript variant X2, mRNA.

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CloneID	OTg04645
Clone ID Related Accession (Same CDS sequence)	XM_008193560.2
Accession Version	XM_008193560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	protein bark beetle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450161.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008193560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGTT TTTTGAGGAC GTTGCTTTTG GCCATCTGTG TTAGTAATGC CGTTCTGCAA 
GAAACGGAAG TGTTTTTTGG GAGTACGGAA GGGTCTAGGG GTGGCAGGAG TGAGCATCCT 
GGAGGGACGA TAGTTAACGA TAAGTTGGTT TTTTCGAAAT TTAAGAGTCC CTACTGGTTA 
AGGAATGACA TCATCGTTGA GAGAAACGCT GAATTAATTA TTGAACCAGG AGTAACCATC 
AACGTTGAAC CGCAGGTTGG AATTACCGTT CGAGGAGTTT TGACTGCTGT GGGTACCGAA 
AATGAACGAA TCGTTCTAAC GACCGCAGAG GAAACCTCTG CGAAGAATAT TAATTTCCCC 
GACATCCGGC TCTCTGACGG CCCCACGATC TTAGCGGGCC GTGTCCAAGT GCGTCACAAT 
GGCCGTTGGC GCAGCGTTTG CACCAACTCC AAAAATTGGA CGCGTGCCGA CATGGAAACG 
GCTTGCCGCC AACTCGGTTT CCAGGGCGGC TCGTTTTTCA ATTGGTTCGA CCGACAAATG 
CCCCTCAAAC CCCGACTTCT TTACGAAGAA CCCAAATGTT CAGGAACGGA AAGCTCTCTG 
TTCGACTGCG ACTGGAACTC GCGCCAAATG GGGGCCGGCG TGTGCGACTA TCACCCGGAC 
TTGGCCATCC AGTGTAGCCC CAGACACGAC AGAAATTTAC CGTTCTGGAG AGGCATCCGT 
TTTGAGGATG CTAAGCATAA TAAAGTCTTG ACACAGTCAA ACACTTTGTA TGTTCCTGTC 
TCGGTGTCGA AGCTACAATA CGTACAGATT TTATACGCGG GGGCCGGACG TGGCCAGAAC 
ACGTCCAGTG CTGTTGAAGT TGAGGGGATT CCCCCAGTTA TGGATAATCT AGAAATTATT 
AACTCGGCCT ACAATGGCAT TAACATAACC AGGCCGGAGG GACCCTTCAG TGTGACCAAC 
TGCACGCTGA GGGGTAACCG CGGTTATGGC ATTTTTGTCA ACTCCAGCCA TGGCTTAACC 
CATATCGATG GGTGCACTAT TAACGAAAAT GGGGGCGATG GGGTTAGATA CGTCCATGGG 
GAGGAACGTC CCGATGAAAG GAGCGATCGC TTCGGTTACG ACGATTTTTG TAACCTTGCG 
ATCGTTTCAA GTCAAACGTT CCCCTTGCAA CTTTTCGCCG AACAAACGCT GTTTCACAAC 
CAAATCAAAT CGTGCGATAA AGTCTTTTCG ACACGTAACC GCAACGTTAT CACCTTATCA 
GTCATTCGGG CTGTCACAAA TTTTAATAAC AGTGCCTCTC TGGAAGTCTA CGACGGCTCC 
ACTTTAAATC ACCGCTTACT TACCTCTTTC CCGATCAGAA ATAACACCAG ACCTCAGTCC 
ATTACAAGCA CTGGACAACA CCTTTTCGTA AAGTTTCGCG CAGCTGCGAG TACCGAAACC 
GTTGTCTTCA TGCGGGTTTT CGCAAGCCCC AGCAAAAGTT ACGACCTAAA TGTTAGTAAT 
TCGGACGTTT CCGAAAACAT GGGGCGCGGG ATCGCCGTTG ATAATTTGCG GTCACAAGTC 
CACGTTTACA AATCTTCGGT TTCGAAAAAC CAACATGTGG CTGGTCTTCA TGTTACGAGC 
GGCGTTGGCG ATGTTAACGT TACCGAAAGT CGCATTTCGT TCAATGAGGG TGATGGGATC 
AACATCACTT ATACTGGCGG TAGCAGGAAC GTCTCCAAGT CGTCAATTAG TTCAAACACT 
GGCTATGGAC TAGCCATTTG GCTGAACGAC ACCAAAGAAA CCGAATTTGT CACAGTGAAC 
CAAACCACAG TCGTCCAGTA TTCAGAGATT TACAAGAATC TGGACACGGG GATTTTACAC 
GGCAATTTCT GCGGCGAGTC TTTTTTTAAC TTTACGGGAA ACCGCTTCCG GAATTGTGCA 
AATGACGCGC TGGAAGTCCT CACCTGTTGG CGGAACACCA ACACTTCGAC GAGACTCCAA 
ATTGGCCATA ACGAATTTAT TGGTAATAAC AGGATCGGGT TGAGGCTAAC CCCAGCTTTG 
AACCTTGAGG CCACAATCGA ATACAACCAC TTCCGTCAAG GGTTATTCGG CGCTCTTTTA 
ATCAAAAACA AGCCTTTGGA GGAGTTTAAT GTCTTGAAAA CCAATATTCA AGTCTTCCAA 
AACTATTTTA TCAATAATAC GGGCACGTTT GTGGCCAACT TGGCCCTGTC ACCTTATGCC 
GAAAACCAAA ACTTGTTGTT TATCCGCAAC TACGTTAAAA ACAACCGGAT CTCGGAACCC 
TTCCAGCCGG AAGACGGCTC AATCTCTAGT CTGAACCCCC GGAGTCGGGT CGCTGCCCCT 
ATAGTAGTGG GGTCGGACAA TGTCGATATT TACCGGAACA TTATTGAAAA CCCTGACTCG 
AAATACGAAA TTGGGAGCCA CCTGGAGGAC CAAAGCAAGT CGCTTAACTG CACGTTTAAT 
TGGCTAGGGT TCGGGCACGA TGAGGAGATT CTACCGCGAC TTTTCCACCG TCTTGACCGT 
TATAATTTGG CCAAAATTGA GTTCACTCCA TTTTTATTGC ACAATACTAA TCCTCTGACC 
AACCGAATTA ACCAATACCA GTACTACATC CCGAAGTTTG TGTCCCCCAG CTCGAATAAA 
GTCGGGGGTG AAGTTGAAGG TGAGGAGACT TTGACTCCCG GTGAATATGT CGTGGAGAAA 
GACATTAATA TACGACCTGG GGGTAAATTG TCAATTGAAC CGGGGGTCAT TCTTAGGTTC 
CCACCTTCGG TGGGAATGAT GGTTGGCGGG AAACTTGAAG CTAGGGGTAT AGGCCCTGAT 
AGTATTAAAC TCACACTGAA GGAAGAGATT TTGCATGTGC CTGATAACGA AACGTACGAA 
ATTGAAACGG AAAAATACGA AGTCGAGACT GAAGTTGTGC AAATCGAGCC TAGAGTCCCG 
ATCCGACTGC TTGGGGGAGC GACGGAGACC GAAGGCCGTC TCCAGGTCAG AATCAACAAC 
GAGTGGGGCA CTGTCTGCAA CTATGGCTGG ACCATGACAA ACGCGGCCCT CGTGTGCCAA 
CAGCTGGGCT TTGTCTTGAA CCCCGAAGAC TGGTTCTTGA AACCTAGCGA GACCCCAGCC 
GCTGGCACGA GTGAAAATAT AATCTTGTCC AACGTCCAGT GTGACGATTT TGACTTGGAC 
GTCACCAAAT GTAAAGCCGA GACCAAAGGC AATTTCGAGA ACGCCTGCAC CCACCAGAAC 
GACGTGGGGA TCAGGTGCTA CAAATCGTCC TGGGCTGGGG TCCGCTTTGG GGCCCTGGCC 
GAACGAAGCA ACATCCAGTA TATCACAGTG GAAATGGCAG GACTCCTCGA TTACGCCACT 
CGTACCTTCA AGCCCGCAGT CCAGATTGAC TTTGCGAGGC AAAATTTCGA AAACATCCGA 
GTTTCCAATA ATTACTACCA CGGGTTGGGC GTCCTCTACG CCGATATTTA CACCGATGAT 
ACAATCAACA TAATTAAAAA CTCGCAGTTT TTGAACAATA AAGGCGCCGG TGTTAGTTTC 
AAGCAACTAG GGTTCAACAT TTTCGGTAGT TTGATTGAGA ACAATTACTT CGGTGTTAAG 
CACAATCCCG TCCTGACCGG GCTCCAGCAA CGCGAGTTCG GGGGTTGGTT CCTCACAAGT 
GACGATAACG TGCGGTACAA ACCGCTAATG ATCCCCGAAA CTTTGGAGGT GAACACCATA 
CAAGTCGACC GCGGGGAAAC GAAGTACTTG GTCACGTCCA AAGTCATTGG GGATGATATC 
TCGCGGGTTT ACCGCTTCCG GAGCGACCCA GGCTGGGTGA TTGGCATGCA ACTTCTGAAC 
CCGATTGAGA ATCGGAGCAC TGAAAGTATC GTCATTCAAG ACGCACTTTC GTACAACAAA 
AACGCCATTT ATTGGGTGCT TAAGCGCGAC CTGACTGTCT TCCCGACAAC GTCGAGTAGT 
TACGGGATTA TTCTGGAGTA CAATAGTGGG AGTTATGCCA TCGGTGGTAC TGTTATTGTG 
ATTAGTACGA TCAGGGCGCC GGTTCAGAAT ATACGCAACC GGATTGTCAA AGGCCCAGTC 
CCGACTCTAC GATTGGTTAA TTCCAAAATC AGGAGTAACC AATTTGGAAT ACACGCCTCG 
TACTATAACA GATATTTGAA CGAGCTGGGT GACCATTTTT TGCGCAAAGC TAACGAAAGT 
TTCCAGCTGG TCAATTGTGA GGTTTCGCAC AACTTGAACG AAGCGATTTT TGTGCATTCG 
CCCCATTGGG ACTTGCATAA GAGTAACCTT TCGGAGGTTG TTATCATGAT TAATAATTCA 
TTGATTACGG ACAATGGCAA GGGTTTTTAC CACTTTTCGC GCGACATGCG CGCCTCTAAC 
AACATTTTCC ATTACATTTT GCAAGACGAT ACAATTGAGA GAAACCGCGC TGGTGGGTTC 
GATGTCAATC TGCCGTACGT TTGGCAGTAC AATGAAAACT TCACCCATTC GGTTTACATG 
GATAACAACA CCTGGACGCA CAACCGTAAC TTCAAATTCA ACATTGACGG CCACTTCGCC 
GTGGTTAACG TCACCCGTAA CGTCTTCACC GATAATAATT GTGCCACCGG TTTGATCACA 
ATCGGCGGAA TGGAGAAAAA ACTATTGATA AGCCGTAACA AGTTCAATAC GAACAACGGC 
AAATTCATCG TAGAGTTTGT CTCAAACAGC CAGTCGGAGA TAATCGGCGA AGTCCCAGCC 
CAGTTCACTT TCAACGAACT CCGCCACAAC AAGTTTGCGT TCGGGGGCCG CAGCTTCGGC 
CTCTTGCAAC TCAAGAAAGA CCCCACGTCG GTGGTCGCCT TCAAAGGCAT CCAAAAAGTC 
AAAATTTTCC GAAACCTATT TTCGCAAAAC CAGCTCGATT ATCAACTGAT CGCGGGGATC 
AAAACCGCCA AAATCGACAC TTTTGTGGAC GTGCGCGAAA ATTGGTGGGG CACGAACGAC 
GAGGTGGAGA TCAAGGAGAA GATTTTCGAT TTCGACGACT GGAACAACTA TGCCATTGCC 
AAGTTCAAGC CCTACTTGGT CAATGACGAT TTCGCGTCGT CTTATTCCGC TGGTTATATT 
AGTGCCACCA CCACTGATTT GAACAAACTT GGGGGGCGGA TCACCCAGGA CTTGGTCTTG 
CATAATGAGA GAAATGCTCC GTATGTCGTC CAGTCCGACA TAACCGTTAT GCCAAAAGTC 
ACCCTGACAA TTGACCCGGG GGTGGTTCTG GAGTTTGCGC CAAACGTTGG TATCCTGGTC 
TTGGGGAGTT TGCAAGCGCG GGGTTACGAA GGCAACGAGA TTATCATGCG GCCGATTTCC 
AAAACGGGTT ATTTGGAGTC GAGTTCGACA GTTCGGGAAA AACGCCAAAT TGAGAATTTG 
GTCGGTGGCG AAAGCATCCG TTTGTGCAAG GGCCGGGGGT GTTACGACGG CGAGGAGAGC 
ACCCCACGGG AGGGTTTCCT CGAATATTTC AATAAAACCA CCCTCCAGTG GATCCCCATG 
TGCGATACAA GGTTTACGGA GCGGAACGCG CAAGTTGTGT GCCGGGAGTT GGGTTTTGAC 
CCACTTAACG TGTTTTTCGA CCATGATGTC AGGATCGAGT TTCATAGTAA CTCGCTTTCG 
CGGATTTGGA CCTGGCCTGA GCCGCTCCAG TGCAAAGGAA CGGAGAAAAA ATACGAGGAC 
TGTCCCATCA GACTGAATGG GCAACAGTTC GGACACCGCC ACCGGTGCAA GTGGAACTCT 
AAGTTTGTGT TCATTCACTG TGGGCGAAGG AATTTGGAGC CGAAGTATGA TTATTGGGGC 
GGTGTTAGGT TTGCCAATCC TGAGTTTGAG CAACAGTTGT ACGCTCATCG CATTCACGAC 
GTGAAAACAC ACTCGACCAT GCAGAGTCAG GAGTCGGTGA TGGAGTATGT TAATATAATC 
GGGGCTGGGA TTTTGCATAA TGAGAAATCG CCGGCGATTC AGAGCATTAT TAAGAGCCCC 
AAGATTAATT TCGTGAGGGT TTACAAAAGT GCCTCACATG GGATTAATTT GATTTCGCCT 
TCGAACACAA TGAATCTGTT GTATAACACG GTCGAGGATT CGCTGGGTGT GGGCATAAAT 
ACGTTGTCAG TGACCGGCGA AGGGAGAGAA TCGCAAGAAT CAAGCTTCAC TCCCTTGAAA 
AGTTTGAACA TTCCCTACAA TCTGTTCAGT CTGGTTGACA TGTGCGACAC CCACAAGGAG 
ATAGAGATCC AGGAGCGCAT CCTGCTGTAC TACAAGTACG ACAATCACCC AGTCAATTGC 
ATCAAAATTT TCAAGAGCGC CTACAACATC AAACCGCTCG GCTTCCGTTT GCTACAGTTC 
AACTTGTTTA ACTCGACCCA AAAATACGGA ATCCCCGATT TTATCTCACT GTACGACGGT 
GATATTTACA ACGTCACCTC GAAACTTATC GACCGCATAA CCATGATGTC CCACAACGAG 
AAAAGACTAG TCCGAACCAA ATTGCCAAGC CTTAGTATCA AACTTTTCGC AAATGGAGCT 
TCGTCCAATC ACGGTTTTAT CGCCGAAGTT GTCACACTTC CAATCTCAGC CATCGGCTTC 
AATCGGGACG TGCAACACAA CATTTCTTAT TCCGTGGTGA ACAACAACAG GGAAGGCGCA 
ATGTTGTACA TGTCAGCGGG CGAAGTCAAC CCGATTGTGA CCATTGACAA GAACCAGTTT 
AGATACAATT GTGAGAAGCT GTACGGGAAT TTCACGACTT GTGATGCCGC CGTGGAGATC 
GATATACAAA ACACCCAGAC TATTTTTTTC AGAAATAATT TGGTTGAAGG TAACCAAGGG 
GGGTTGGCGA TCAAGGCGGA TTCGCGCGGC TCAGCCACCT CTCTCAAGGG TTACATCCAC 
AATAATTTGT TTGTGAATAA TAGCAACTTG CCGGTGTTGT ACGTGGAAGG ACGGCAGTCG 
TCCCCCTATC AAGAAGTGGT GATTTACCGA AACTACTTTA CGAGGAACAA AGCACAATAC 
TACAATAATA TTGTTCTGAA ACAGGTCGTT TCCAACTTTA CGCTAAATTA CGTGAAGAGG 
AATGTCGGTT TGAAAAATTT GGAAGTGTCA GGGTTTGATA AAGTCAGGCT TCCGATTTAC 
CAAACGACGT CTCATAATGG GTTTTATCAT AATTATGCCA TTCATCGCGA TAGCAGATCG 
ACGATTGTGG CGGGGACTGC AGGTCAACAT TACGTGGATA ATATCTTCTT TAACCCAGAT 
AATGATTATG AAATGATAAC AGTAAACAGA TCTCTCACTT TACAATTATG GAACACAAAA 
ATCGACGCTG CTTACAACTA CTGGGGCCTT AACAACTCTC TGGGTGTCCG AGGTCGCATC 
AAGGACCAAA GCGACGATCC TCGTCTCCTT GAAGTTATTT ACGAGCCTTA TCACATGAAC 
AATAAATCAG TTTTACTCAA TAAAAAATGT CCCCCGGGAT GGGATCTCGT CGGAGGGACT 
TGTTATATTT ATATTGGTGC TCCCATGACC TTCCATGAAG CTCGAGCTTT TTGTCAGGCT 
GATAACGCAA CAATGCCGTA TTTGTTAGGC AATTTGAATT ACATCGAACT GTATGAGTTT 
TTACGATCGC AACAACAGTG GTTTTTGTAC AATGATAGGA TTTGGGTGCA ACATATCGAC 
AAAATCAACG AGTGCACCAT GTTTGCCTAC CAAAGGGTCG AGGTTGATAA CTGCGATCAA 
AGGAGTCCCT TCATCTGCGA AATAGATCCT AAAGTTTCGA TAAACATCCT GCCATTGACC 
GACGATGTTG TTACAATAGC CGTTCTGTCA AGCCTGGCTT TGGCCTTACT TTTGATGGCC 
ATAGTCGGTG TTTTTTGGTG GAGCAAATCA AAGTATCGCC AAACCCAAAG GCTGGAAAGA 
CGGAACAGTA TCAGGCAAAG TTTACATTCA TTGCGCTCCG TTGGCCTGGC ACCTGGCAGT 
TTCTCTGACG CTAGTTACCG CCGAAAAGTG GCACAACTTA GCTCGCGCTC AACCGACACA 
CTAACAAAAA ATTCCGAATA CAAGAAAATG ATAACGAATG GATCACTAGA CAGTATGGAA 
AAATCCACCT ACACGTCGTC CCTTGACGAC AACCAGTCGT ATGACGTTTA CGAGGCCCAT 
AACCCTAACC CTTCCGCTTT TGCTTATAGC CCTACAATTG AGTATCACAA ATCACCAAAT 
AGGTTCGATG CCCAGTATGC CAAACCTAAC GGGTTCGAAC TGGCGTACAA AAACGAGGGT 
TTTCGCGATA ATTCGACTTT TGCAACGAAT TCAAATTATC AATCACGGGC CGAAAGCATC 
CAAGACAGTA CCAACGACGA CATCCCGATT ATCCAAGACA ACGTTGGCGG GAACAGTGTT 
TCCTACCCCC CTAGTGAATA CTACACCACT GACACGCTCC CCCTGAGCGG AAAATCGGAC 
TCAACCCTTG ACCTGAAGAA GGGAATTGAT GATTATAATA AAAACCGCAA CTACGACCCG 
ATCTACAGTC GCGAAAGACC AAATGCGAAT TTGTTGAACG AGTTGAAAAA TAAATTCCCG 
CAAACGCCGC CGCCGCCACC AGACCCGGTC CAATCGCCCA CTTATTCGGA AAAGTTAGAC 
AGTCTGCAAT ACTCCCCTGA TTATAACACG GTGGCGTTAG CTGAGCCCTT CTCTGCCAAT 
ATACTTGAGA CCGACTTTGA CGAGCCGCAC CCACGGCCGA AAGTTCGGGC CAAAAGTGAG 
GTGCTGCTAG AGACTAACTT TGACTACACT CCGCCTAGTG GTAGTCCGCA GTTGAATCAC 
CCAATCACCG AATATAATAG AAGTAAGAGT CAACCGTTGG AGACAGCGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008191782.1
CDS	405..9278
Translation	MCSFLRTLLLAICVSNAVLQETEVFFGSTEGSRGGRSEHPGGTIVNDKLVFSKFKSPYWLRNDIIVERNAELIIEPGVTINVEPQVGITVRGVLTAVGTENERIVLTTAEETSAKNINFPDIRLSDGPTILAGRVQVRHNGRWRSVCTNSKNWTRADMETACRQLGFQGGSFFNWFDRQMPLKPRLLYEEPKCSGTESSLFDCDWNSRQMGAGVCDYHPDLAIQCSPRHDRNLPFWRGIRFEDAKHNKVLTQSNTLYVPVSVSKLQYVQILYAGAGRGQNTSSAVEVEGIPPVMDNLEIINSAYNGINITRPEGPFSVTNCTLRGNRGYGIFVNSSHGLTHIDGCTINENGGDGVRYVHGEERPDERSDRFGYDDFCNLAIVSSQTFPLQLFAEQTLFHNQIKSCDKVFSTRNRNVITLSVIRAVTNFNNSASLEVYDGSTLNHRLLTSFPIRNNTRPQSITSTGQHLFVKFRAAASTETVVFMRVFASPSKSYDLNVSNSDVSENMGRGIAVDNLRSQVHVYKSSVSKNQHVAGLHVTSGVGDVNVTESRISFNEGDGINITYTGGSRNVSKSSISSNTGYGLAIWLNDTKETEFVTVNQTTVVQYSEIYKNLDTGILHGNFCGESFFNFTGNRFRNCANDALEVLTCWRNTNTSTRLQIGHNEFIGNNRIGLRLTPALNLEATIEYNHFRQGLFGALLIKNKPLEEFNVLKTNIQVFQNYFINNTGTFVANLALSPYAENQNLLFIRNYVKNNRISEPFQPEDGSISSLNPRSRVAAPIVVGSDNVDIYRNIIENPDSKYEIGSHLEDQSKSLNCTFNWLGFGHDEEILPRLFHRLDRYNLAKIEFTPFLLHNTNPLTNRINQYQYYIPKFVSPSSNKVGGEVEGEETLTPGEYVVEKDINIRPGGKLSIEPGVILRFPPSVGMMVGGKLEARGIGPDSIKLTLKEEILHVPDNETYEIETEKYEVETEVVQIEPRVPIRLLGGATETEGRLQVRINNEWGTVCNYGWTMTNAALVCQQLGFVLNPEDWFLKPSETPAAGTSENIILSNVQCDDFDLDVTKCKAETKGNFENACTHQNDVGIRCYKSSWAGVRFGALAERSNIQYITVEMAGLLDYATRTFKPAVQIDFARQNFENIRVSNNYYHGLGVLYADIYTDDTINIIKNSQFLNNKGAGVSFKQLGFNIFGSLIENNYFGVKHNPVLTGLQQREFGGWFLTSDDNVRYKPLMIPETLEVNTIQVDRGETKYLVTSKVIGDDISRVYRFRSDPGWVIGMQLLNPIENRSTESIVIQDALSYNKNAIYWVLKRDLTVFPTTSSSYGIILEYNSGSYAIGGTVIVISTIRAPVQNIRNRIVKGPVPTLRLVNSKIRSNQFGIHASYYNRYLNELGDHFLRKANESFQLVNCEVSHNLNEAIFVHSPHWDLHKSNLSEVVIMINNSLITDNGKGFYHFSRDMRASNNIFHYILQDDTIERNRAGGFDVNLPYVWQYNENFTHSVYMDNNTWTHNRNFKFNIDGHFAVVNVTRNVFTDNNCATGLITIGGMEKKLLISRNKFNTNNGKFIVEFVSNSQSEIIGEVPAQFTFNELRHNKFAFGGRSFGLLQLKKDPTSVVAFKGIQKVKIFRNLFSQNQLDYQLIAGIKTAKIDTFVDVRENWWGTNDEVEIKEKIFDFDDWNNYAIAKFKPYLVNDDFASSYSAGYISATTTDLNKLGGRITQDLVLHNERNAPYVVQSDITVMPKVTLTIDPGVVLEFAPNVGILVLGSLQARGYEGNEIIMRPISKTGYLESSSTVREKRQIENLVGGESIRLCKGRGCYDGEESTPREGFLEYFNKTTLQWIPMCDTRFTERNAQVVCRELGFDPLNVFFDHDVRIEFHSNSLSRIWTWPEPLQCKGTEKKYEDCPIRLNGQQFGHRHRCKWNSKFVFIHCGRRNLEPKYDYWGGVRFANPEFEQQLYAHRIHDVKTHSTMQSQESVMEYVNIIGAGILHNEKSPAIQSIIKSPKINFVRVYKSASHGINLISPSNTMNLLYNTVEDSLGVGINTLSVTGEGRESQESSFTPLKSLNIPYNLFSLVDMCDTHKEIEIQERILLYYKYDNHPVNCIKIFKSAYNIKPLGFRLLQFNLFNSTQKYGIPDFISLYDGDIYNVTSKLIDRITMMSHNEKRLVRTKLPSLSIKLFANGASSNHGFIAEVVTLPISAIGFNRDVQHNISYSVVNNNREGAMLYMSAGEVNPIVTIDKNQFRYNCEKLYGNFTTCDAAVEIDIQNTQTIFFRNNLVEGNQGGLAIKADSRGSATSLKGYIHNNLFVNNSNLPVLYVEGRQSSPYQEVVIYRNYFTRNKAQYYNNIVLKQVVSNFTLNYVKRNVGLKNLEVSGFDKVRLPIYQTTSHNGFYHNYAIHRDSRSTIVAGTAGQHYVDNIFFNPDNDYEMITVNRSLTLQLWNTKIDAAYNYWGLNNSLGVRGRIKDQSDDPRLLEVIYEPYHMNNKSVLLNKKCPPGWDLVGGTCYIYIGAPMTFHEARAFCQADNATMPYLLGNLNYIELYEFLRSQQQWFLYNDRIWVQHIDKINECTMFAYQRVEVDNCDQRSPFICEIDPKVSINILPLTDDVVTIAVLSSLALALLLMAIVGVFWWSKSKYRQTQRLERRNSIRQSLHSLRSVGLAPGSFSDASYRRKVAQLSSRSTDTLTKNSEYKKMITNGSLDSMEKSTYTSSLDDNQSYDVYEAHNPNPSAFAYSPTIEYHKSPNRFDAQYAKPNGFELAYKNEGFRDNSTFATNSNYQSRAESIQDSTNDDIPIIQDNVGGNSVSYPPSEYYTTDTLPLSGKSDSTLDLKKGIDDYNKNRNYDPIYSRERPNANLLNELKNKFPQTPPPPPDPVQSPTYSEKLDSLQYSPDYNTVALAEPFSANILETDFDEPHPRPKVRAKSEVLLETNFDYTPPSGSPQLNHPITEYNRSKSQPLETAM

Target ORF information:

RefSeq Version	XM_008193560.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum protein bark beetle (LOC657327), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008193560.2

ORF Insert Sequence:

ATGTGTAGTT TTTTGAGGAC GTTGCTTTTG GCCATCTGTG TTAGTAATGC CGTTCTGCAA 
GAAACGGAAG TGTTTTTTGG GAGTACGGAA GGGTCTAGGG GTGGCAGGAG TGAGCATCCT 
GGAGGGACGA TAGTTAACGA TAAGTTGGTT TTTTCGAAAT TTAAGAGTCC CTACTGGTTA 
AGGAATGACA TCATCGTTGA GAGAAACGCT GAATTAATTA TTGAACCAGG AGTAACCATC 
AACGTTGAAC CGCAGGTTGG AATTACCGTT CGAGGAGTTT TGACTGCTGT GGGTACCGAA 
AATGAACGAA TCGTTCTAAC GACCGCAGAG GAAACCTCTG CGAAGAATAT TAATTTCCCC 
GACATCCGGC TCTCTGACGG CCCCACGATC TTAGCGGGCC GTGTCCAAGT GCGTCACAAT 
GGCCGTTGGC GCAGCGTTTG CACCAACTCC AAAAATTGGA CGCGTGCCGA CATGGAAACG 
GCTTGCCGCC AACTCGGTTT CCAGGGCGGC TCGTTTTTCA ATTGGTTCGA CCGACAAATG 
CCCCTCAAAC CCCGACTTCT TTACGAAGAA CCCAAATGTT CAGGAACGGA AAGCTCTCTG 
TTCGACTGCG ACTGGAACTC GCGCCAAATG GGGGCCGGCG TGTGCGACTA TCACCCGGAC 
TTGGCCATCC AGTGTAGCCC CAGACACGAC AGAAATTTAC CGTTCTGGAG AGGCATCCGT 
TTTGAGGATG CTAAGCATAA TAAAGTCTTG ACACAGTCAA ACACTTTGTA TGTTCCTGTC 
TCGGTGTCGA AGCTACAATA CGTACAGATT TTATACGCGG GGGCCGGACG TGGCCAGAAC 
ACGTCCAGTG CTGTTGAAGT TGAGGGGATT CCCCCAGTTA TGGATAATCT AGAAATTATT 
AACTCGGCCT ACAATGGCAT TAACATAACC AGGCCGGAGG GACCCTTCAG TGTGACCAAC 
TGCACGCTGA GGGGTAACCG CGGTTATGGC ATTTTTGTCA ACTCCAGCCA TGGCTTAACC 
CATATCGATG GGTGCACTAT TAACGAAAAT GGGGGCGATG GGGTTAGATA CGTCCATGGG 
GAGGAACGTC CCGATGAAAG GAGCGATCGC TTCGGTTACG ACGATTTTTG TAACCTTGCG 
ATCGTTTCAA GTCAAACGTT CCCCTTGCAA CTTTTCGCCG AACAAACGCT GTTTCACAAC 
CAAATCAAAT CGTGCGATAA AGTCTTTTCG ACACGTAACC GCAACGTTAT CACCTTATCA 
GTCATTCGGG CTGTCACAAA TTTTAATAAC AGTGCCTCTC TGGAAGTCTA CGACGGCTCC 
ACTTTAAATC ACCGCTTACT TACCTCTTTC CCGATCAGAA ATAACACCAG ACCTCAGTCC 
ATTACAAGCA CTGGACAACA CCTTTTCGTA AAGTTTCGCG CAGCTGCGAG TACCGAAACC 
GTTGTCTTCA TGCGGGTTTT CGCAAGCCCC AGCAAAAGTT ACGACCTAAA TGTTAGTAAT 
TCGGACGTTT CCGAAAACAT GGGGCGCGGG ATCGCCGTTG ATAATTTGCG GTCACAAGTC 
CACGTTTACA AATCTTCGGT TTCGAAAAAC CAACATGTGG CTGGTCTTCA TGTTACGAGC 
GGCGTTGGCG ATGTTAACGT TACCGAAAGT CGCATTTCGT TCAATGAGGG TGATGGGATC 
AACATCACTT ATACTGGCGG TAGCAGGAAC GTCTCCAAGT CGTCAATTAG TTCAAACACT 
GGCTATGGAC TAGCCATTTG GCTGAACGAC ACCAAAGAAA CCGAATTTGT CACAGTGAAC 
CAAACCACAG TCGTCCAGTA TTCAGAGATT TACAAGAATC TGGACACGGG GATTTTACAC 
GGCAATTTCT GCGGCGAGTC TTTTTTTAAC TTTACGGGAA ACCGCTTCCG GAATTGTGCA 
AATGACGCGC TGGAAGTCCT CACCTGTTGG CGGAACACCA ACACTTCGAC GAGACTCCAA 
ATTGGCCATA ACGAATTTAT TGGTAATAAC AGGATCGGGT TGAGGCTAAC CCCAGCTTTG 
AACCTTGAGG CCACAATCGA ATACAACCAC TTCCGTCAAG GGTTATTCGG CGCTCTTTTA 
ATCAAAAACA AGCCTTTGGA GGAGTTTAAT GTCTTGAAAA CCAATATTCA AGTCTTCCAA 
AACTATTTTA TCAATAATAC GGGCACGTTT GTGGCCAACT TGGCCCTGTC ACCTTATGCC 
GAAAACCAAA ACTTGTTGTT TATCCGCAAC TACGTTAAAA ACAACCGGAT CTCGGAACCC 
TTCCAGCCGG AAGACGGCTC AATCTCTAGT CTGAACCCCC GGAGTCGGGT CGCTGCCCCT 
ATAGTAGTGG GGTCGGACAA TGTCGATATT TACCGGAACA TTATTGAAAA CCCTGACTCG 
AAATACGAAA TTGGGAGCCA CCTGGAGGAC CAAAGCAAGT CGCTTAACTG CACGTTTAAT 
TGGCTAGGGT TCGGGCACGA TGAGGAGATT CTACCGCGAC TTTTCCACCG TCTTGACCGT 
TATAATTTGG CCAAAATTGA GTTCACTCCA TTTTTATTGC ACAATACTAA TCCTCTGACC 
AACCGAATTA ACCAATACCA GTACTACATC CCGAAGTTTG TGTCCCCCAG CTCGAATAAA 
GTCGGGGGTG AAGTTGAAGG TGAGGAGACT TTGACTCCCG GTGAATATGT CGTGGAGAAA 
GACATTAATA TACGACCTGG GGGTAAATTG TCAATTGAAC CGGGGGTCAT TCTTAGGTTC 
CCACCTTCGG TGGGAATGAT GGTTGGCGGG AAACTTGAAG CTAGGGGTAT AGGCCCTGAT 
AGTATTAAAC TCACACTGAA GGAAGAGATT TTGCATGTGC CTGATAACGA AACGTACGAA 
ATTGAAACGG AAAAATACGA AGTCGAGACT GAAGTTGTGC AAATCGAGCC TAGAGTCCCG 
ATCCGACTGC TTGGGGGAGC GACGGAGACC GAAGGCCGTC TCCAGGTCAG AATCAACAAC 
GAGTGGGGCA CTGTCTGCAA CTATGGCTGG ACCATGACAA ACGCGGCCCT CGTGTGCCAA 
CAGCTGGGCT TTGTCTTGAA CCCCGAAGAC TGGTTCTTGA AACCTAGCGA GACCCCAGCC 
GCTGGCACGA GTGAAAATAT AATCTTGTCC AACGTCCAGT GTGACGATTT TGACTTGGAC 
GTCACCAAAT GTAAAGCCGA GACCAAAGGC AATTTCGAGA ACGCCTGCAC CCACCAGAAC 
GACGTGGGGA TCAGGTGCTA CAAATCGTCC TGGGCTGGGG TCCGCTTTGG GGCCCTGGCC 
GAACGAAGCA ACATCCAGTA TATCACAGTG GAAATGGCAG GACTCCTCGA TTACGCCACT 
CGTACCTTCA AGCCCGCAGT CCAGATTGAC TTTGCGAGGC AAAATTTCGA AAACATCCGA 
GTTTCCAATA ATTACTACCA CGGGTTGGGC GTCCTCTACG CCGATATTTA CACCGATGAT 
ACAATCAACA TAATTAAAAA CTCGCAGTTT TTGAACAATA AAGGCGCCGG TGTTAGTTTC 
AAGCAACTAG GGTTCAACAT TTTCGGTAGT TTGATTGAGA ACAATTACTT CGGTGTTAAG 
CACAATCCCG TCCTGACCGG GCTCCAGCAA CGCGAGTTCG GGGGTTGGTT CCTCACAAGT 
GACGATAACG TGCGGTACAA ACCGCTAATG ATCCCCGAAA CTTTGGAGGT GAACACCATA 
CAAGTCGACC GCGGGGAAAC GAAGTACTTG GTCACGTCCA AAGTCATTGG GGATGATATC 
TCGCGGGTTT ACCGCTTCCG GAGCGACCCA GGCTGGGTGA TTGGCATGCA ACTTCTGAAC 
CCGATTGAGA ATCGGAGCAC TGAAAGTATC GTCATTCAAG ACGCACTTTC GTACAACAAA 
AACGCCATTT ATTGGGTGCT TAAGCGCGAC CTGACTGTCT TCCCGACAAC GTCGAGTAGT 
TACGGGATTA TTCTGGAGTA CAATAGTGGG AGTTATGCCA TCGGTGGTAC TGTTATTGTG 
ATTAGTACGA TCAGGGCGCC GGTTCAGAAT ATACGCAACC GGATTGTCAA AGGCCCAGTC 
CCGACTCTAC GATTGGTTAA TTCCAAAATC AGGAGTAACC AATTTGGAAT ACACGCCTCG 
TACTATAACA GATATTTGAA CGAGCTGGGT GACCATTTTT TGCGCAAAGC TAACGAAAGT 
TTCCAGCTGG TCAATTGTGA GGTTTCGCAC AACTTGAACG AAGCGATTTT TGTGCATTCG 
CCCCATTGGG ACTTGCATAA GAGTAACCTT TCGGAGGTTG TTATCATGAT TAATAATTCA 
TTGATTACGG ACAATGGCAA GGGTTTTTAC CACTTTTCGC GCGACATGCG CGCCTCTAAC 
AACATTTTCC ATTACATTTT GCAAGACGAT ACAATTGAGA GAAACCGCGC TGGTGGGTTC 
GATGTCAATC TGCCGTACGT TTGGCAGTAC AATGAAAACT TCACCCATTC GGTTTACATG 
GATAACAACA CCTGGACGCA CAACCGTAAC TTCAAATTCA ACATTGACGG CCACTTCGCC 
GTGGTTAACG TCACCCGTAA CGTCTTCACC GATAATAATT GTGCCACCGG TTTGATCACA 
ATCGGCGGAA TGGAGAAAAA ACTATTGATA AGCCGTAACA AGTTCAATAC GAACAACGGC 
AAATTCATCG TAGAGTTTGT CTCAAACAGC CAGTCGGAGA TAATCGGCGA AGTCCCAGCC 
CAGTTCACTT TCAACGAACT CCGCCACAAC AAGTTTGCGT TCGGGGGCCG CAGCTTCGGC 
CTCTTGCAAC TCAAGAAAGA CCCCACGTCG GTGGTCGCCT TCAAAGGCAT CCAAAAAGTC 
AAAATTTTCC GAAACCTATT TTCGCAAAAC CAGCTCGATT ATCAACTGAT CGCGGGGATC 
AAAACCGCCA AAATCGACAC TTTTGTGGAC GTGCGCGAAA ATTGGTGGGG CACGAACGAC 
GAGGTGGAGA TCAAGGAGAA GATTTTCGAT TTCGACGACT GGAACAACTA TGCCATTGCC 
AAGTTCAAGC CCTACTTGGT CAATGACGAT TTCGCGTCGT CTTATTCCGC TGGTTATATT 
AGTGCCACCA CCACTGATTT GAACAAACTT GGGGGGCGGA TCACCCAGGA CTTGGTCTTG 
CATAATGAGA GAAATGCTCC GTATGTCGTC CAGTCCGACA TAACCGTTAT GCCAAAAGTC 
ACCCTGACAA TTGACCCGGG GGTGGTTCTG GAGTTTGCGC CAAACGTTGG TATCCTGGTC 
TTGGGGAGTT TGCAAGCGCG GGGTTACGAA GGCAACGAGA TTATCATGCG GCCGATTTCC 
AAAACGGGTT ATTTGGAGTC GAGTTCGACA GTTCGGGAAA AACGCCAAAT TGAGAATTTG 
GTCGGTGGCG AAAGCATCCG TTTGTGCAAG GGCCGGGGGT GTTACGACGG CGAGGAGAGC 
ACCCCACGGG AGGGTTTCCT CGAATATTTC AATAAAACCA CCCTCCAGTG GATCCCCATG 
TGCGATACAA GGTTTACGGA GCGGAACGCG CAAGTTGTGT GCCGGGAGTT GGGTTTTGAC 
CCACTTAACG TGTTTTTCGA CCATGATGTC AGGATCGAGT TTCATAGTAA CTCGCTTTCG 
CGGATTTGGA CCTGGCCTGA GCCGCTCCAG TGCAAAGGAA CGGAGAAAAA ATACGAGGAC 
TGTCCCATCA GACTGAATGG GCAACAGTTC GGACACCGCC ACCGGTGCAA GTGGAACTCT 
AAGTTTGTGT TCATTCACTG TGGGCGAAGG AATTTGGAGC CGAAGTATGA TTATTGGGGC 
GGTGTTAGGT TTGCCAATCC TGAGTTTGAG CAACAGTTGT ACGCTCATCG CATTCACGAC 
GTGAAAACAC ACTCGACCAT GCAGAGTCAG GAGTCGGTGA TGGAGTATGT TAATATAATC 
GGGGCTGGGA TTTTGCATAA TGAGAAATCG CCGGCGATTC AGAGCATTAT TAAGAGCCCC 
AAGATTAATT TCGTGAGGGT TTACAAAAGT GCCTCACATG GGATTAATTT GATTTCGCCT 
TCGAACACAA TGAATCTGTT GTATAACACG GTCGAGGATT CGCTGGGTGT GGGCATAAAT 
ACGTTGTCAG TGACCGGCGA AGGGAGAGAA TCGCAAGAAT CAAGCTTCAC TCCCTTGAAA 
AGTTTGAACA TTCCCTACAA TCTGTTCAGT CTGGTTGACA TGTGCGACAC CCACAAGGAG 
ATAGAGATCC AGGAGCGCAT CCTGCTGTAC TACAAGTACG ACAATCACCC AGTCAATTGC 
ATCAAAATTT TCAAGAGCGC CTACAACATC AAACCGCTCG GCTTCCGTTT GCTACAGTTC 
AACTTGTTTA ACTCGACCCA AAAATACGGA ATCCCCGATT TTATCTCACT GTACGACGGT 
GATATTTACA ACGTCACCTC GAAACTTATC GACCGCATAA CCATGATGTC CCACAACGAG 
AAAAGACTAG TCCGAACCAA ATTGCCAAGC CTTAGTATCA AACTTTTCGC AAATGGAGCT 
TCGTCCAATC ACGGTTTTAT CGCCGAAGTT GTCACACTTC CAATCTCAGC CATCGGCTTC 
AATCGGGACG TGCAACACAA CATTTCTTAT TCCGTGGTGA ACAACAACAG GGAAGGCGCA 
ATGTTGTACA TGTCAGCGGG CGAAGTCAAC CCGATTGTGA CCATTGACAA GAACCAGTTT 
AGATACAATT GTGAGAAGCT GTACGGGAAT TTCACGACTT GTGATGCCGC CGTGGAGATC 
GATATACAAA ACACCCAGAC TATTTTTTTC AGAAATAATT TGGTTGAAGG TAACCAAGGG 
GGGTTGGCGA TCAAGGCGGA TTCGCGCGGC TCAGCCACCT CTCTCAAGGG TTACATCCAC 
AATAATTTGT TTGTGAATAA TAGCAACTTG CCGGTGTTGT ACGTGGAAGG ACGGCAGTCG 
TCCCCCTATC AAGAAGTGGT GATTTACCGA AACTACTTTA CGAGGAACAA AGCACAATAC 
TACAATAATA TTGTTCTGAA ACAGGTCGTT TCCAACTTTA CGCTAAATTA CGTGAAGAGG 
AATGTCGGTT TGAAAAATTT GGAAGTGTCA GGGTTTGATA AAGTCAGGCT TCCGATTTAC 
CAAACGACGT CTCATAATGG GTTTTATCAT AATTATGCCA TTCATCGCGA TAGCAGATCG 
ACGATTGTGG CGGGGACTGC AGGTCAACAT TACGTGGATA ATATCTTCTT TAACCCAGAT 
AATGATTATG AAATGATAAC AGTAAACAGA TCTCTCACTT TACAATTATG GAACACAAAA 
ATCGACGCTG CTTACAACTA CTGGGGCCTT AACAACTCTC TGGGTGTCCG AGGTCGCATC 
AAGGACCAAA GCGACGATCC TCGTCTCCTT GAAGTTATTT ACGAGCCTTA TCACATGAAC 
AATAAATCAG TTTTACTCAA TAAAAAATGT CCCCCGGGAT GGGATCTCGT CGGAGGGACT 
TGTTATATTT ATATTGGTGC TCCCATGACC TTCCATGAAG CTCGAGCTTT TTGTCAGGCT 
GATAACGCAA CAATGCCGTA TTTGTTAGGC AATTTGAATT ACATCGAACT GTATGAGTTT 
TTACGATCGC AACAACAGTG GTTTTTGTAC AATGATAGGA TTTGGGTGCA ACATATCGAC 
AAAATCAACG AGTGCACCAT GTTTGCCTAC CAAAGGGTCG AGGTTGATAA CTGCGATCAA 
AGGAGTCCCT TCATCTGCGA AATAGATCCT AAAGTTTCGA TAAACATCCT GCCATTGACC 
GACGATGTTG TTACAATAGC CGTTCTGTCA AGCCTGGCTT TGGCCTTACT TTTGATGGCC 
ATAGTCGGTG TTTTTTGGTG GAGCAAATCA AAGTATCGCC AAACCCAAAG GCTGGAAAGA 
CGGAACAGTA TCAGGCAAAG TTTACATTCA TTGCGCTCCG TTGGCCTGGC ACCTGGCAGT 
TTCTCTGACG CTAGTTACCG CCGAAAAGTG GCACAACTTA GCTCGCGCTC AACCGACACA 
CTAACAAAAA ATTCCGAATA CAAGAAAATG ATAACGAATG GATCACTAGA CAGTATGGAA 
AAATCCACCT ACACGTCGTC CCTTGACGAC AACCAGTCGT ATGACGTTTA CGAGGCCCAT 
AACCCTAACC CTTCCGCTTT TGCTTATAGC CCTACAATTG AGTATCACAA ATCACCAAAT 
AGGTTCGATG CCCAGTATGC CAAACCTAAC GGGTTCGAAC TGGCGTACAA AAACGAGGGT 
TTTCGCGATA ATTCGACTTT TGCAACGAAT TCAAATTATC AATCACGGGC CGAAAGCATC 
CAAGACAGTA CCAACGACGA CATCCCGATT ATCCAAGACA ACGTTGGCGG GAACAGTGTT 
TCCTACCCCC CTAGTGAATA CTACACCACT GACACGCTCC CCCTGAGCGG AAAATCGGAC 
TCAACCCTTG ACCTGAAGAA GGGAATTGAT GATTATAATA AAAACCGCAA CTACGACCCG 
ATCTACAGTC GCGAAAGACC AAATGCGAAT TTGTTGAACG AGTTGAAAAA TAAATTCCCG 
CAAACGCCGC CGCCGCCACC AGACCCGGTC CAATCGCCCA CTTATTCGGA AAAGTTAGAC 
AGTCTGCAAT ACTCCCCTGA TTATAACACG GTGGCGTTAG CTGAGCCCTT CTCTGCCAAT 
ATACTTGAGA CCGACTTTGA CGAGCCGCAC CCACGGCCGA AAGTTCGGGC CAAAAGTGAG 
GTGCTGCTAG AGACTAACTT TGACTACACT CCGCCTAGTG GTAGTCCGCA GTTGAATCAC 
CCAATCACCG AATATAATAG AAGTAAGAGT CAACCGTTGG AGACAGCGAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC657327 ( XM_008193561.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008191783.1 Tribolium castaneum protein bark beetle (LOC657327), transcript variant X3, mRNA.

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CloneID	OTg04646
Clone ID Related Accession (Same CDS sequence)	XM_008193561.2
Accession Version	XM_008193561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	protein bark beetle isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450161.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008193561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGTT TTTTGAGGAC GTTGCTTTTG GCCATCTGTG TTAGTAATGC CGTTCTGCAA 
GAAACGGAAG TGTTTTTTGG GAGTACGGAA GGGTCTAGGG GTGGCAGGAG TGAGCATCCT 
GGAGGGACGA TAGTTAACGA TAAGTTGGTT TTTTCGAAAT TTAAGAGTCC CTACTGGTTA 
AGGAATGACA TCATCGTTGA GAGAAACGCT GAATTAATTA TTGAACCAGG AGTAACCATC 
AACGTTGAAC CGCAGGTTGG AATTACCGTT CGAGGAGTTT TGACTGCTGT GGGTACCGAA 
AATGAACGAA TCGTTCTAAC GACCGCAGAG GAAACCTCTG CGAAGAATAT TAATTTCCCC 
GACATCCGGC TCTCTGACGG CCCCACGATC TTAGCGGGCC GTGTCCAAGT GCGTCACAAT 
GGCCGTTGGC GCAGCGTTTG CACCAACTCC AAAAATTGGA CGCGTGCCGA CATGGAAACG 
GCTTGCCGCC AACTCGGTTT CCAGGGCGGC TCGTTTTTCA ATTGGTTCGA CCGACAAATG 
CCCCTCAAAC CCCGACTTCT TTACGAAGAA CCCAAATGTT CAGGAACGGA AAGCTCTCTG 
TTCGACTGCG ACTGGAACTC GCGCCAAATG GGGGCCGGCG TGTGCGACTA TCACCCGGAC 
TTGGCCATCC AGTGTAGCCC CAGACACGAC AGAAATTTAC CGTTCTGGAG AGGCATCCGT 
TTTGAGGATG CTAAGCATAA TAAAGTCTTG ACACAGTCAA ACACTTTGTA TGTTCCTGTC 
TCGGTGTCGA AGCTACAATA CGTACAGATT TTATACGCGG GGGCCGGACG TGGCCAGAAC 
ACGTCCAGTG CTGTTGAAGT TGAGGGGATT CCCCCAGTTA TGGATAATCT AGAAATTATT 
AACTCGGCCT ACAATGGCAT TAACATAACC AGGCCGGAGG GACCCTTCAG TGTGACCAAC 
TGCACGCTGA GGGGTAACCG CGGTTATGGC ATTTTTGTCA ACTCCAGCCA TGGCTTAACC 
CATATCGATG GGTGCACTAT TAACGAAAAT GGGGGCGATG GGGTTAGATA CGTCCATGGG 
GAGGAACGTC CCGATGAAAG GAGCGATCGC TTCGGTTACG ACGATTTTTG TAACCTTGCG 
ATCGTTTCAA GTCAAACGTT CCCCTTGCAA CTTTTCGCCG AACAAACGCT GTTTCACAAC 
CAAATCAAAT CGTGCGATAA AGTCTTTTCG ACACGTAACC GCAACGTTAT CACCTTATCA 
GTCATTCGGG CTGTCACAAA TTTTAATAAC AGTGCCTCTC TGGAAGTCTA CGACGGCTCC 
ACTTTAAATC ACCGCTTACT TACCTCTTTC CCGATCAGAA ATAACACCAG ACCTCAGTCC 
ATTACAAGCA CTGGACAACA CCTTTTCGTA AAGTTTCGCG CAGCTGCGAG TACCGAAACC 
GTTGTCTTCA TGCGGGTTTT CGCAAGCCCC AGCAAAAGTT ACGACCTAAA TGTTAGTAAT 
TCGGACGTTT CCGAAAACAT GGGGCGCGGG ATCGCCGTTG ATAATTTGCG GTCACAAGTC 
CACGTTTACA AATCTTCGGT TTCGAAAAAC CAACATGTGG CTGGTCTTCA TGTTACGAGC 
GGCGTTGGCG ATGTTAACGT TACCGAAAGT CGCATTTCGT TCAATGAGGG TGATGGGATC 
AACATCACTT ATACTGGCGG TAGCAGGAAC GTCTCCAAGT CGTCAATTAG TTCAAACACT 
GGCTATGGAC TAGCCATTTG GCTGAACGAC ACCAAAGAAA CCGAATTTGT CACAGTGAAC 
CAAACCACAG TCGTCCAGTA TTCAGAGATT TACAAGAATC TGGACACGGG GATTTTACAC 
GGCAATTTCT GCGGCGAGTC TTTTTTTAAC TTTACGGGAA ACCGCTTCCG GAATTGTGCA 
AATGACGCGC TGGAAGTCCT CACCTGTTGG CGGAACACCA ACACTTCGAC GAGACTCCAA 
ATTGGCCATA ACGAATTTAT TGGTAATAAC AGGATCGGGT TGAGGCTAAC CCCAGCTTTG 
AACCTTGAGG CCACAATCGA ATACAACCAC TTCCGTCAAG GGTTATTCGG CGCTCTTTTA 
ATCAAAAACA AGCCTTTGGA GGAGTTTAAT GTCTTGAAAA CCAATATTCA AGTCTTCCAA 
AACTATTTTA TCAATAATAC GGGCACGTTT GTGGCCAACT TGGCCCTGTC ACCTTATGCC 
GAAAACCAAA ACTTGTTGTT TATCCGCAAC TACGTTAAAA ACAACCGGAT CTCGGAACCC 
TTCCAGCCGG AAGACGGCTC AATCTCTAGT CTGAACCCCC GGAGTCGGGT CGCTGCCCCT 
ATAGTAGTGG GGTCGGACAA TGTCGATATT TACCGGAACA TTATTGAAAA CCCTGACTCG 
AAATACGAAA TTGGGAGCCA CCTGGAGGAC CAAAGCAAGT CGCTTAACTG CACGTTTAAT 
TGGCTAGGGT TCGGGCACGA TGAGGAGATT CTACCGCGAC TTTTCCACCG TCTTGACCGT 
TATAATTTGG CCAAAATTGA GTTCACTCCA TTTTTATTGC ACAATACTAA TCCTCTGACC 
AACCGAATTA ACCAATACCA GTACTACATC CCGAAGTTTG TGTCCCCCAG CTCGAATAAA 
GTCGGGGGTG AAGTTGAAGG TGAGGAGACT TTGACTCCCG GTGAATATGT CGTGGAGAAA 
GACATTAATA TACGACCTGG GGGTAAATTG TCAATTGAAC CGGGGGTCAT TCTTAGGTTC 
CCACCTTCGG TGGGAATGAT GGTTGGCGGG AAACTTGAAG CTAGGGGTAT AGGCCCTGAT 
AGTATTAAAC TCACACTGAA GGAAGAGATT TTGCATGTGC CTGATAACGA AACGTACGAA 
ATTGAAACGG AAAAATACGA AGTCGAGACT GAAGTTGTGC AAATCGAGCC TAGAGTCCCG 
ATCCGACTGC TTGGGGGAGC GACGGAGACC GAAGGCCGTC TCCAGGTCAG AATCAACAAC 
GAGTGGGGCA CTGTCTGCAA CTATGGCTGG ACCATGACAA ACGCGGCCCT CGTGTGCCAA 
CAGCTGGGCT TTGTCTTGAA CCCCGAAGAC TGGTTCTTGA AACCTAGCGA GACCCCAGCC 
GCTGGCACGA GTGAAAATAT AATCTTGTCC AACGTCCAGT GTGACGATTT TGACTTGGAC 
GTCACCAAAT GTAAAGCCGA GACCAAAGGC AATTTCGAGA ACGCCTGCAC CCACCAGAAC 
GACGTGGGGA TCAGGTGCTA CAAATCGTCC TGGGCTGGGG TCCGCTTTGG GGCCCTGGCC 
GAACGAAGCA ACATCCAGTA TATCACAGTG GAAATGGCAG GACTCCTCGA TTACGCCACT 
CGTACCTTCA AGCCCGCAGT CCAGATTGAC TTTGCGAGGC AAAATTTCGA AAACATCCGA 
GTTTCCAATA ATTACTACCA CGGGTTGGGC GTCCTCTACG CCGATATTTA CACCGATGAT 
ACAATCAACA TAATTAAAAA CTCGCAGTTT TTGAACAATA AAGGCGCCGG TGTTAGTTTC 
AAGCAACTAG GGTTCAACAT TTTCGGTAGT TTGATTGAGA ACAATTACTT CGGTGTTAAG 
CACAATCCCG TCCTGACCGG GCTCCAGCAA CGCGAGTTCG GGGGTTGGTT CCTCACAAGT 
GACGATAACG TGCGGTACAA ACCGCTAATG ATCCCCGAAA CTTTGGAGGT GAACACCATA 
CAAGTCGACC GCGGGGAAAC GAAGTACTTG GTCACGTCCA AAGTCATTGG GGATGATATC 
TCGCGGGTTT ACCGCTTCCG GAGCGACCCA GGCTGGGTGA TTGGCATGCA ACTTCTGAAC 
CCGATTGAGA ATCGGAGCAC TGAAAGTATC GTCATTCAAG ACGCACTTTC GTACAACAAA 
AACGCCATTT ATTGGGTGCT TAAGCGCGAC CTGACTGTCT TCCCGACAAC GTCGAGTAGT 
TACGGGATTA TTCTGGAGTA CAATAGTGGG AGTTATGCCA TCGGTGGTAC TGTTATTGTG 
ATTAGTACGA TCAGGGCGCC GGTTCAGAAT ATACGCAACC GGATTGTCAA AGGCCCAGTC 
CCGACTCTAC GATTGGTTAA TTCCAAAATC AGGAGTAACC AATTTGGAAT ACACGCCTCG 
TACTATAACA GATATTTGAA CGAGCTGGGT GACCATTTTT TGCGCAAAGC TAACGAAAGT 
TTCCAGCTGG TCAATTGTGA GGTTTCGCAC AACTTGAACG AAGCGATTTT TGTGCATTCG 
CCCCATTGGG ACTTGCATAA GAGTAACCTT TCGGAGGTTG TTATCATGAT TAATAATTCA 
TTGATTACGG ACAATGGCAA GGGTTTTTAC CACTTTTCGC GCGACATGCG CGCCTCTAAC 
AACATTTTCC ATTACATTTT GCAAGACGAT ACAATTGAGA GAAACCGCGC TGGTGGGTTC 
GATGTCAATC TGCCGTACGT TTGGCAGTAC AATGAAAACT TCACCCATTC GGTTTACATG 
GATAACAACA CCTGGACGCA CAACCGTAAC TTCAAATTCA ACATTGACGG CCACTTCGCC 
GTGGTTAACG TCACCCGTAA CGTCTTCACC GATAATAATT GTGCCACCGG TTTGATCACA 
ATCGGCGGAA TGGAGAAAAA ACTATTGATA AGCCGTAACA AGTTCAATAC GAACAACGGC 
AAATTCATCG TAGAGTTTGT CTCAAACAGC CAGTCGGAGA TAATCGGCGA AGTCCCAGCC 
CAGTTCACTT TCAACGAACT CCGCCACAAC AAGTTTGCGT TCGGGGGCCG CAGCTTCGGC 
CTCTTGCAAC TCAAGAAAGA CCCCACGTCG GTGGTCGCCT TCAAAGGCAT CCAAAAAGTC 
AAAATTTTCC GAAACCTATT TTCGCAAAAC CAGCTCGATT ATCAACTGAT CGCGGGGATC 
AAAACCGCCA AAATCGACAC TTTTGTGGAC GTGCGCGAAA ATTGGTGGGG CACGAACGAC 
GAGGTGGAGA TCAAGGAGAA GATTTTCGAT TTCGACGACT GGAACAACTA TGCCATTGCC 
AAGTTCAAGC CCTACTTGGT CAATGACGAT TTCGCGTCGT CTTATTCCGC TGGTTATATT 
AGTGCCACCA CCACTGATTT GAACAAACTT GGGGGGCGGA TCACCCAGGA CTTGGTCTTG 
CATAATGAGA GAAATGCTCC GTATGTCGTC CAGTCCGACA TAACCGTTAT GCCAAAAGTC 
ACCCTGACAA TTGACCCGGG GGTGGTTCTG GAGTTTGCGC CAAACGTTGG TATCCTGGTC 
TTGGGGAGTT TGCAAGCGCG GGGTTACGAA GGCAACGAGA TTATCATGCG GCCGATTTCC 
AAAACGGGTT ATTTGGAGTC GAGTTCGACA GTTCGGGAAA AACGCCAAAT TGAGAATTTG 
GTCGGTGGCG AAAGCATCCG TTTGTGCAAG GGCCGGGGGT GTTACGACGG CGAGGAGAGC 
ACCCCACGGG AGGGTTTCCT CGAATATTTC AATAAAACCA CCCTCCAGTG GATCCCCATG 
TGCGATACAA GGTTTACGGA GCGGAACGCG CAAGTTGTGT GCCGGGAGTT GGGTTTTGAC 
CCACTTAACG TGTTTTTCGA CCATGATGTC AGGATCGAGT TTCATAGTAA CTCGCTTTCG 
CGGATTTGGA CCTGGCCTGA GCCGCTCCAG TGCAAAGGAA CGGAGAAAAA ATACGAGGAC 
TGTCCCATCA GACTGAATGG GCAACAGTTC GGACACCGCC ACCGGTGCAA GTGGAACTCT 
AAGTTTGTGT TCATTCACTG TGGGCGAAGG AATTTGGAGC CGAAGTATGA TTATTGGGGC 
GGTGTTAGGT TTGCCAATCC TGAGTTTGAG CAACAGTTGT ACGCTCATCG CATTCACGAC 
GTGAAAACAC ACTCGACCAT GCAGAGTCAG GAGTCGGTGA TGGAGTATGT TAATATAATC 
GGGGCTGGGA TTTTGCATAA TGAGAAATCG CCGGCGATTC AGAGCATTAT TAAGAGCCCC 
AAGATTAATT TCGTGAGGGT TTACAAAAGT GCCTCACATG GGATTAATTT GATTTCGCCT 
TCGAACACAA TGAATCTGTT GTATAACACG GTCGAGGATT CGCTGGGTGT GGGCATAAAT 
ACGTTGTCAG TGACCGGCGA AGGGAGAGAA TCGCAAGAAT CAAGCTTCAC TCCCTTGAAA 
AGTTTGAACA TTCCCTACAA TCTGTTCAGT CTGGTTGACA TGTGCGACAC CCACAAGGAG 
ATAGAGATCC AGGAGCGCAT CCTGCTGTAC TACAAGTACG ACAATCACCC AGTCAATTGC 
ATCAAAATTT TCAAGAGCGC CTACAACATC AAACCGCTCG GCTTCCGTTT GCTACAGTTC 
AACTTGTTTA ACTCGACCCA AAAATACGGA ATCCCCGATT TTATCTCACT GTACGACGGT 
GATATTTACA ACGTCACCTC GAAACTTATC GACCGCATAA CCATGATGTC CCACAACGAG 
AAAAGACTAG TCCGAACCAA ATTGCCAAGC CTTAGTATCA AACTTTTCGC AAATGGAGCT 
TCGTCCAATC ACGGTTTTAT CGCCGAAGTT GTCACACTTC CAATCTCAGC CATCGGCTTC 
AATCGGGACG TGCAACACAA CATTTCTTAT TCCGTGGTGA ACAACAACAG GGAAGGCGCA 
ATGTTGTACA TGTCAGCGGG CGAAGTCAAC CCGATTGTGA CCATTGACAA GAACCAGTTT 
AGATACAATT GTGAGAAGCT GTACGGGAAT TTCACGACTT GTGATGCCGC CGTGGAGATC 
GATATACAAA ACACCCAGAC TATTTTTTTC AGAAATAATT TGGTTGAAGG TAACCAAGGG 
GGGTTGGCGA TCAAGGCGGA TTCGCGCGGC TCAGCCACCT CTCTCAAGGG TTACATCCAC 
AATAATTTGT TTGTGAATAA TAGCAACTTG CCGGTGTTGT ACGTGGAAGG ACGGCAGTCG 
TCCCCCTATC AAGAAGTGGT GATTTACCGA AACTACTTTA CGAGGAACAA AGCACAATAC 
TACAATAATA TTGTTCTGAA ACAGGTCGTT TCCAACTTTA CGCTAAATTA CGTGAAGAGG 
AATGTCGGTT TGAAAAATTT GGAAGTGTCA GGGTTTGATA AAGTCAGGCT TCCGATTTAC 
CAAACGACGT CTCATAATGG GTTTTATCAT AATTATGCCA TTCATCGCGA TAGCAGATCG 
ACGATTGTGG CGGGGACTGC AGGTCAACAT TACGTGGATA ATATCTTCTT TAACCCAGAT 
AATGATTATG AAATGATAAC AGTAAACAGA TCTCTATTTG AATTCAACAG CACTTTACAA 
TTATGGAACA CAAAAATCGA CGCTGCTTAC AACTACTGGG GCCTTAACAA CTCTCTGGGT 
GTCCGAGGTC GCATCAAGGA CCAAAGCGAC GATCCTCGTC TCCTTGAAGT TATTTACGAG 
CCTTATCACA TGAACAATAA ATCAGTTTTA CTCAATAAAA AATGTCCCCC GGGATGGGAT 
CTCGTCGGAG GGACTTGTTA TATTTATATT GGTGCTCCCA TGACCTTCCA TGAAGCTCGA 
GCTTTTTGTC AGGCTGATAA CGCAACAATG CCGTATTTGT TAGGCAATTT GAATTACATC 
GAACTGTATG AGTTTTTACG ATCGCAACAA CAGTGGTTTT TGTACAATGA TAGGATTTGG 
GTGCAACATA TCGACAAAAT CAACGAGTGC ACCATGTTTG CCTACCAAAG GGTCGAGGTT 
GATAACTGCG ATCAAAGGAG TCCCTTCATC TGCGAAATAG ATCCTAAAGT TTCGATAAAC 
ATCCTGCCAT TGACCGACGA TGTTGTTACA ATAGCCGTTC TGTCAAGCCT GGCTTTGGCC 
TTACTTTTGA TGGCCATAGT CGGTGTTTTT TGGTGGAGCA AATCAAAGTA TCGCCAAACC 
CAAAGGCTGG AAAGACGGAA CAGTATCAGG CAAAGTTTAC ATTCATTGCG CTCCGTTGGC 
CTGGCACCTG GCAGTTTCTC TGACGCTAGT TACCGCCGAA AAGTGGCACA ACTTAGCTCG 
CGCTCAACCG ACACACTAAC AAAAAATTCC GAATACAAGA AAATGATAAC GAATGGATCA 
CTAGACAGTA TGGAAAAATC CACCTACACG TCGTCCCTTG ACGACAACCA GTCCCCTACA 
ATTGAGTATC ACAAATCACC AAATAGGTTC GATGCCCAGT ATGCCAAACC TAACGGGTTC 
GAACTGGCGT ACAAAAACGA GGGTTTTCGC GATAATTCGA CTTTTGCAAC GAATTCAAAT 
TATCAATCAC GGGCCGAAAG CATCCAAGAC AGTACCAACG ACGACATCCC GATTATCCAA 
GACAACGTTG GCGGGAACAG TGTTTCCTAC CCCCCTAGTG AATACTACAC CACTGACACG 
CTCCCCCTGA GCGGAAAATC GGACTCAACC CTTGACCTGA AGAAGGGAAT TGATGATTAT 
AATAAAAACC GCAACTACGA CCCGATCTAC AGTCGCGAAA GACCAAATGC GAATTTGTTG 
AACGAGTTGA AAAATAAATT CCCGCAAACG CCGCCGCCGC CACCAGACCC GGTCCAATCG 
CCCACTTATT CGGAAAAGTT AGACAGTCTG CAATACTCCC CTGATTATAA CACGGTGGCG 
TTAGCTGAGC CCTTCTCTGC CAATATACTT GAGACCGACT TTGACGAGCC GCACCCACGG 
CCGAAAGTTC GGGCCAAAAG TGAGGTGCTG CTAGAGACTA ACTTTGACTA CACTCCGCCT 
AGTGGTAGTC CGCAGTTGAA TCACCCAATC ACCGAATATA ATAGAAGTAA GAGTCAACCG 
TTGGAGACAG CGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008191783.1
CDS	405..9242
Translation	MCSFLRTLLLAICVSNAVLQETEVFFGSTEGSRGGRSEHPGGTIVNDKLVFSKFKSPYWLRNDIIVERNAELIIEPGVTINVEPQVGITVRGVLTAVGTENERIVLTTAEETSAKNINFPDIRLSDGPTILAGRVQVRHNGRWRSVCTNSKNWTRADMETACRQLGFQGGSFFNWFDRQMPLKPRLLYEEPKCSGTESSLFDCDWNSRQMGAGVCDYHPDLAIQCSPRHDRNLPFWRGIRFEDAKHNKVLTQSNTLYVPVSVSKLQYVQILYAGAGRGQNTSSAVEVEGIPPVMDNLEIINSAYNGINITRPEGPFSVTNCTLRGNRGYGIFVNSSHGLTHIDGCTINENGGDGVRYVHGEERPDERSDRFGYDDFCNLAIVSSQTFPLQLFAEQTLFHNQIKSCDKVFSTRNRNVITLSVIRAVTNFNNSASLEVYDGSTLNHRLLTSFPIRNNTRPQSITSTGQHLFVKFRAAASTETVVFMRVFASPSKSYDLNVSNSDVSENMGRGIAVDNLRSQVHVYKSSVSKNQHVAGLHVTSGVGDVNVTESRISFNEGDGINITYTGGSRNVSKSSISSNTGYGLAIWLNDTKETEFVTVNQTTVVQYSEIYKNLDTGILHGNFCGESFFNFTGNRFRNCANDALEVLTCWRNTNTSTRLQIGHNEFIGNNRIGLRLTPALNLEATIEYNHFRQGLFGALLIKNKPLEEFNVLKTNIQVFQNYFINNTGTFVANLALSPYAENQNLLFIRNYVKNNRISEPFQPEDGSISSLNPRSRVAAPIVVGSDNVDIYRNIIENPDSKYEIGSHLEDQSKSLNCTFNWLGFGHDEEILPRLFHRLDRYNLAKIEFTPFLLHNTNPLTNRINQYQYYIPKFVSPSSNKVGGEVEGEETLTPGEYVVEKDINIRPGGKLSIEPGVILRFPPSVGMMVGGKLEARGIGPDSIKLTLKEEILHVPDNETYEIETEKYEVETEVVQIEPRVPIRLLGGATETEGRLQVRINNEWGTVCNYGWTMTNAALVCQQLGFVLNPEDWFLKPSETPAAGTSENIILSNVQCDDFDLDVTKCKAETKGNFENACTHQNDVGIRCYKSSWAGVRFGALAERSNIQYITVEMAGLLDYATRTFKPAVQIDFARQNFENIRVSNNYYHGLGVLYADIYTDDTINIIKNSQFLNNKGAGVSFKQLGFNIFGSLIENNYFGVKHNPVLTGLQQREFGGWFLTSDDNVRYKPLMIPETLEVNTIQVDRGETKYLVTSKVIGDDISRVYRFRSDPGWVIGMQLLNPIENRSTESIVIQDALSYNKNAIYWVLKRDLTVFPTTSSSYGIILEYNSGSYAIGGTVIVISTIRAPVQNIRNRIVKGPVPTLRLVNSKIRSNQFGIHASYYNRYLNELGDHFLRKANESFQLVNCEVSHNLNEAIFVHSPHWDLHKSNLSEVVIMINNSLITDNGKGFYHFSRDMRASNNIFHYILQDDTIERNRAGGFDVNLPYVWQYNENFTHSVYMDNNTWTHNRNFKFNIDGHFAVVNVTRNVFTDNNCATGLITIGGMEKKLLISRNKFNTNNGKFIVEFVSNSQSEIIGEVPAQFTFNELRHNKFAFGGRSFGLLQLKKDPTSVVAFKGIQKVKIFRNLFSQNQLDYQLIAGIKTAKIDTFVDVRENWWGTNDEVEIKEKIFDFDDWNNYAIAKFKPYLVNDDFASSYSAGYISATTTDLNKLGGRITQDLVLHNERNAPYVVQSDITVMPKVTLTIDPGVVLEFAPNVGILVLGSLQARGYEGNEIIMRPISKTGYLESSSTVREKRQIENLVGGESIRLCKGRGCYDGEESTPREGFLEYFNKTTLQWIPMCDTRFTERNAQVVCRELGFDPLNVFFDHDVRIEFHSNSLSRIWTWPEPLQCKGTEKKYEDCPIRLNGQQFGHRHRCKWNSKFVFIHCGRRNLEPKYDYWGGVRFANPEFEQQLYAHRIHDVKTHSTMQSQESVMEYVNIIGAGILHNEKSPAIQSIIKSPKINFVRVYKSASHGINLISPSNTMNLLYNTVEDSLGVGINTLSVTGEGRESQESSFTPLKSLNIPYNLFSLVDMCDTHKEIEIQERILLYYKYDNHPVNCIKIFKSAYNIKPLGFRLLQFNLFNSTQKYGIPDFISLYDGDIYNVTSKLIDRITMMSHNEKRLVRTKLPSLSIKLFANGASSNHGFIAEVVTLPISAIGFNRDVQHNISYSVVNNNREGAMLYMSAGEVNPIVTIDKNQFRYNCEKLYGNFTTCDAAVEIDIQNTQTIFFRNNLVEGNQGGLAIKADSRGSATSLKGYIHNNLFVNNSNLPVLYVEGRQSSPYQEVVIYRNYFTRNKAQYYNNIVLKQVVSNFTLNYVKRNVGLKNLEVSGFDKVRLPIYQTTSHNGFYHNYAIHRDSRSTIVAGTAGQHYVDNIFFNPDNDYEMITVNRSLFEFNSTLQLWNTKIDAAYNYWGLNNSLGVRGRIKDQSDDPRLLEVIYEPYHMNNKSVLLNKKCPPGWDLVGGTCYIYIGAPMTFHEARAFCQADNATMPYLLGNLNYIELYEFLRSQQQWFLYNDRIWVQHIDKINECTMFAYQRVEVDNCDQRSPFICEIDPKVSINILPLTDDVVTIAVLSSLALALLLMAIVGVFWWSKSKYRQTQRLERRNSIRQSLHSLRSVGLAPGSFSDASYRRKVAQLSSRSTDTLTKNSEYKKMITNGSLDSMEKSTYTSSLDDNQSPTIEYHKSPNRFDAQYAKPNGFELAYKNEGFRDNSTFATNSNYQSRAESIQDSTNDDIPIIQDNVGGNSVSYPPSEYYTTDTLPLSGKSDSTLDLKKGIDDYNKNRNYDPIYSRERPNANLLNELKNKFPQTPPPPPDPVQSPTYSEKLDSLQYSPDYNTVALAEPFSANILETDFDEPHPRPKVRAKSEVLLETNFDYTPPSGSPQLNHPITEYNRSKSQPLETAM

Target ORF information:

RefSeq Version	XM_008193561.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum protein bark beetle (LOC657327), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008193561.2

ORF Insert Sequence:

ATGTGTAGTT TTTTGAGGAC GTTGCTTTTG GCCATCTGTG TTAGTAATGC CGTTCTGCAA 
GAAACGGAAG TGTTTTTTGG GAGTACGGAA GGGTCTAGGG GTGGCAGGAG TGAGCATCCT 
GGAGGGACGA TAGTTAACGA TAAGTTGGTT TTTTCGAAAT TTAAGAGTCC CTACTGGTTA 
AGGAATGACA TCATCGTTGA GAGAAACGCT GAATTAATTA TTGAACCAGG AGTAACCATC 
AACGTTGAAC CGCAGGTTGG AATTACCGTT CGAGGAGTTT TGACTGCTGT GGGTACCGAA 
AATGAACGAA TCGTTCTAAC GACCGCAGAG GAAACCTCTG CGAAGAATAT TAATTTCCCC 
GACATCCGGC TCTCTGACGG CCCCACGATC TTAGCGGGCC GTGTCCAAGT GCGTCACAAT 
GGCCGTTGGC GCAGCGTTTG CACCAACTCC AAAAATTGGA CGCGTGCCGA CATGGAAACG 
GCTTGCCGCC AACTCGGTTT CCAGGGCGGC TCGTTTTTCA ATTGGTTCGA CCGACAAATG 
CCCCTCAAAC CCCGACTTCT TTACGAAGAA CCCAAATGTT CAGGAACGGA AAGCTCTCTG 
TTCGACTGCG ACTGGAACTC GCGCCAAATG GGGGCCGGCG TGTGCGACTA TCACCCGGAC 
TTGGCCATCC AGTGTAGCCC CAGACACGAC AGAAATTTAC CGTTCTGGAG AGGCATCCGT 
TTTGAGGATG CTAAGCATAA TAAAGTCTTG ACACAGTCAA ACACTTTGTA TGTTCCTGTC 
TCGGTGTCGA AGCTACAATA CGTACAGATT TTATACGCGG GGGCCGGACG TGGCCAGAAC 
ACGTCCAGTG CTGTTGAAGT TGAGGGGATT CCCCCAGTTA TGGATAATCT AGAAATTATT 
AACTCGGCCT ACAATGGCAT TAACATAACC AGGCCGGAGG GACCCTTCAG TGTGACCAAC 
TGCACGCTGA GGGGTAACCG CGGTTATGGC ATTTTTGTCA ACTCCAGCCA TGGCTTAACC 
CATATCGATG GGTGCACTAT TAACGAAAAT GGGGGCGATG GGGTTAGATA CGTCCATGGG 
GAGGAACGTC CCGATGAAAG GAGCGATCGC TTCGGTTACG ACGATTTTTG TAACCTTGCG 
ATCGTTTCAA GTCAAACGTT CCCCTTGCAA CTTTTCGCCG AACAAACGCT GTTTCACAAC 
CAAATCAAAT CGTGCGATAA AGTCTTTTCG ACACGTAACC GCAACGTTAT CACCTTATCA 
GTCATTCGGG CTGTCACAAA TTTTAATAAC AGTGCCTCTC TGGAAGTCTA CGACGGCTCC 
ACTTTAAATC ACCGCTTACT TACCTCTTTC CCGATCAGAA ATAACACCAG ACCTCAGTCC 
ATTACAAGCA CTGGACAACA CCTTTTCGTA AAGTTTCGCG CAGCTGCGAG TACCGAAACC 
GTTGTCTTCA TGCGGGTTTT CGCAAGCCCC AGCAAAAGTT ACGACCTAAA TGTTAGTAAT 
TCGGACGTTT CCGAAAACAT GGGGCGCGGG ATCGCCGTTG ATAATTTGCG GTCACAAGTC 
CACGTTTACA AATCTTCGGT TTCGAAAAAC CAACATGTGG CTGGTCTTCA TGTTACGAGC 
GGCGTTGGCG ATGTTAACGT TACCGAAAGT CGCATTTCGT TCAATGAGGG TGATGGGATC 
AACATCACTT ATACTGGCGG TAGCAGGAAC GTCTCCAAGT CGTCAATTAG TTCAAACACT 
GGCTATGGAC TAGCCATTTG GCTGAACGAC ACCAAAGAAA CCGAATTTGT CACAGTGAAC 
CAAACCACAG TCGTCCAGTA TTCAGAGATT TACAAGAATC TGGACACGGG GATTTTACAC 
GGCAATTTCT GCGGCGAGTC TTTTTTTAAC TTTACGGGAA ACCGCTTCCG GAATTGTGCA 
AATGACGCGC TGGAAGTCCT CACCTGTTGG CGGAACACCA ACACTTCGAC GAGACTCCAA 
ATTGGCCATA ACGAATTTAT TGGTAATAAC AGGATCGGGT TGAGGCTAAC CCCAGCTTTG 
AACCTTGAGG CCACAATCGA ATACAACCAC TTCCGTCAAG GGTTATTCGG CGCTCTTTTA 
ATCAAAAACA AGCCTTTGGA GGAGTTTAAT GTCTTGAAAA CCAATATTCA AGTCTTCCAA 
AACTATTTTA TCAATAATAC GGGCACGTTT GTGGCCAACT TGGCCCTGTC ACCTTATGCC 
GAAAACCAAA ACTTGTTGTT TATCCGCAAC TACGTTAAAA ACAACCGGAT CTCGGAACCC 
TTCCAGCCGG AAGACGGCTC AATCTCTAGT CTGAACCCCC GGAGTCGGGT CGCTGCCCCT 
ATAGTAGTGG GGTCGGACAA TGTCGATATT TACCGGAACA TTATTGAAAA CCCTGACTCG 
AAATACGAAA TTGGGAGCCA CCTGGAGGAC CAAAGCAAGT CGCTTAACTG CACGTTTAAT 
TGGCTAGGGT TCGGGCACGA TGAGGAGATT CTACCGCGAC TTTTCCACCG TCTTGACCGT 
TATAATTTGG CCAAAATTGA GTTCACTCCA TTTTTATTGC ACAATACTAA TCCTCTGACC 
AACCGAATTA ACCAATACCA GTACTACATC CCGAAGTTTG TGTCCCCCAG CTCGAATAAA 
GTCGGGGGTG AAGTTGAAGG TGAGGAGACT TTGACTCCCG GTGAATATGT CGTGGAGAAA 
GACATTAATA TACGACCTGG GGGTAAATTG TCAATTGAAC CGGGGGTCAT TCTTAGGTTC 
CCACCTTCGG TGGGAATGAT GGTTGGCGGG AAACTTGAAG CTAGGGGTAT AGGCCCTGAT 
AGTATTAAAC TCACACTGAA GGAAGAGATT TTGCATGTGC CTGATAACGA AACGTACGAA 
ATTGAAACGG AAAAATACGA AGTCGAGACT GAAGTTGTGC AAATCGAGCC TAGAGTCCCG 
ATCCGACTGC TTGGGGGAGC GACGGAGACC GAAGGCCGTC TCCAGGTCAG AATCAACAAC 
GAGTGGGGCA CTGTCTGCAA CTATGGCTGG ACCATGACAA ACGCGGCCCT CGTGTGCCAA 
CAGCTGGGCT TTGTCTTGAA CCCCGAAGAC TGGTTCTTGA AACCTAGCGA GACCCCAGCC 
GCTGGCACGA GTGAAAATAT AATCTTGTCC AACGTCCAGT GTGACGATTT TGACTTGGAC 
GTCACCAAAT GTAAAGCCGA GACCAAAGGC AATTTCGAGA ACGCCTGCAC CCACCAGAAC 
GACGTGGGGA TCAGGTGCTA CAAATCGTCC TGGGCTGGGG TCCGCTTTGG GGCCCTGGCC 
GAACGAAGCA ACATCCAGTA TATCACAGTG GAAATGGCAG GACTCCTCGA TTACGCCACT 
CGTACCTTCA AGCCCGCAGT CCAGATTGAC TTTGCGAGGC AAAATTTCGA AAACATCCGA 
GTTTCCAATA ATTACTACCA CGGGTTGGGC GTCCTCTACG CCGATATTTA CACCGATGAT 
ACAATCAACA TAATTAAAAA CTCGCAGTTT TTGAACAATA AAGGCGCCGG TGTTAGTTTC 
AAGCAACTAG GGTTCAACAT TTTCGGTAGT TTGATTGAGA ACAATTACTT CGGTGTTAAG 
CACAATCCCG TCCTGACCGG GCTCCAGCAA CGCGAGTTCG GGGGTTGGTT CCTCACAAGT 
GACGATAACG TGCGGTACAA ACCGCTAATG ATCCCCGAAA CTTTGGAGGT GAACACCATA 
CAAGTCGACC GCGGGGAAAC GAAGTACTTG GTCACGTCCA AAGTCATTGG GGATGATATC 
TCGCGGGTTT ACCGCTTCCG GAGCGACCCA GGCTGGGTGA TTGGCATGCA ACTTCTGAAC 
CCGATTGAGA ATCGGAGCAC TGAAAGTATC GTCATTCAAG ACGCACTTTC GTACAACAAA 
AACGCCATTT ATTGGGTGCT TAAGCGCGAC CTGACTGTCT TCCCGACAAC GTCGAGTAGT 
TACGGGATTA TTCTGGAGTA CAATAGTGGG AGTTATGCCA TCGGTGGTAC TGTTATTGTG 
ATTAGTACGA TCAGGGCGCC GGTTCAGAAT ATACGCAACC GGATTGTCAA AGGCCCAGTC 
CCGACTCTAC GATTGGTTAA TTCCAAAATC AGGAGTAACC AATTTGGAAT ACACGCCTCG 
TACTATAACA GATATTTGAA CGAGCTGGGT GACCATTTTT TGCGCAAAGC TAACGAAAGT 
TTCCAGCTGG TCAATTGTGA GGTTTCGCAC AACTTGAACG AAGCGATTTT TGTGCATTCG 
CCCCATTGGG ACTTGCATAA GAGTAACCTT TCGGAGGTTG TTATCATGAT TAATAATTCA 
TTGATTACGG ACAATGGCAA GGGTTTTTAC CACTTTTCGC GCGACATGCG CGCCTCTAAC 
AACATTTTCC ATTACATTTT GCAAGACGAT ACAATTGAGA GAAACCGCGC TGGTGGGTTC 
GATGTCAATC TGCCGTACGT TTGGCAGTAC AATGAAAACT TCACCCATTC GGTTTACATG 
GATAACAACA CCTGGACGCA CAACCGTAAC TTCAAATTCA ACATTGACGG CCACTTCGCC 
GTGGTTAACG TCACCCGTAA CGTCTTCACC GATAATAATT GTGCCACCGG TTTGATCACA 
ATCGGCGGAA TGGAGAAAAA ACTATTGATA AGCCGTAACA AGTTCAATAC GAACAACGGC 
AAATTCATCG TAGAGTTTGT CTCAAACAGC CAGTCGGAGA TAATCGGCGA AGTCCCAGCC 
CAGTTCACTT TCAACGAACT CCGCCACAAC AAGTTTGCGT TCGGGGGCCG CAGCTTCGGC 
CTCTTGCAAC TCAAGAAAGA CCCCACGTCG GTGGTCGCCT TCAAAGGCAT CCAAAAAGTC 
AAAATTTTCC GAAACCTATT TTCGCAAAAC CAGCTCGATT ATCAACTGAT CGCGGGGATC 
AAAACCGCCA AAATCGACAC TTTTGTGGAC GTGCGCGAAA ATTGGTGGGG CACGAACGAC 
GAGGTGGAGA TCAAGGAGAA GATTTTCGAT TTCGACGACT GGAACAACTA TGCCATTGCC 
AAGTTCAAGC CCTACTTGGT CAATGACGAT TTCGCGTCGT CTTATTCCGC TGGTTATATT 
AGTGCCACCA CCACTGATTT GAACAAACTT GGGGGGCGGA TCACCCAGGA CTTGGTCTTG 
CATAATGAGA GAAATGCTCC GTATGTCGTC CAGTCCGACA TAACCGTTAT GCCAAAAGTC 
ACCCTGACAA TTGACCCGGG GGTGGTTCTG GAGTTTGCGC CAAACGTTGG TATCCTGGTC 
TTGGGGAGTT TGCAAGCGCG GGGTTACGAA GGCAACGAGA TTATCATGCG GCCGATTTCC 
AAAACGGGTT ATTTGGAGTC GAGTTCGACA GTTCGGGAAA AACGCCAAAT TGAGAATTTG 
GTCGGTGGCG AAAGCATCCG TTTGTGCAAG GGCCGGGGGT GTTACGACGG CGAGGAGAGC 
ACCCCACGGG AGGGTTTCCT CGAATATTTC AATAAAACCA CCCTCCAGTG GATCCCCATG 
TGCGATACAA GGTTTACGGA GCGGAACGCG CAAGTTGTGT GCCGGGAGTT GGGTTTTGAC 
CCACTTAACG TGTTTTTCGA CCATGATGTC AGGATCGAGT TTCATAGTAA CTCGCTTTCG 
CGGATTTGGA CCTGGCCTGA GCCGCTCCAG TGCAAAGGAA CGGAGAAAAA ATACGAGGAC 
TGTCCCATCA GACTGAATGG GCAACAGTTC GGACACCGCC ACCGGTGCAA GTGGAACTCT 
AAGTTTGTGT TCATTCACTG TGGGCGAAGG AATTTGGAGC CGAAGTATGA TTATTGGGGC 
GGTGTTAGGT TTGCCAATCC TGAGTTTGAG CAACAGTTGT ACGCTCATCG CATTCACGAC 
GTGAAAACAC ACTCGACCAT GCAGAGTCAG GAGTCGGTGA TGGAGTATGT TAATATAATC 
GGGGCTGGGA TTTTGCATAA TGAGAAATCG CCGGCGATTC AGAGCATTAT TAAGAGCCCC 
AAGATTAATT TCGTGAGGGT TTACAAAAGT GCCTCACATG GGATTAATTT GATTTCGCCT 
TCGAACACAA TGAATCTGTT GTATAACACG GTCGAGGATT CGCTGGGTGT GGGCATAAAT 
ACGTTGTCAG TGACCGGCGA AGGGAGAGAA TCGCAAGAAT CAAGCTTCAC TCCCTTGAAA 
AGTTTGAACA TTCCCTACAA TCTGTTCAGT CTGGTTGACA TGTGCGACAC CCACAAGGAG 
ATAGAGATCC AGGAGCGCAT CCTGCTGTAC TACAAGTACG ACAATCACCC AGTCAATTGC 
ATCAAAATTT TCAAGAGCGC CTACAACATC AAACCGCTCG GCTTCCGTTT GCTACAGTTC 
AACTTGTTTA ACTCGACCCA AAAATACGGA ATCCCCGATT TTATCTCACT GTACGACGGT 
GATATTTACA ACGTCACCTC GAAACTTATC GACCGCATAA CCATGATGTC CCACAACGAG 
AAAAGACTAG TCCGAACCAA ATTGCCAAGC CTTAGTATCA AACTTTTCGC AAATGGAGCT 
TCGTCCAATC ACGGTTTTAT CGCCGAAGTT GTCACACTTC CAATCTCAGC CATCGGCTTC 
AATCGGGACG TGCAACACAA CATTTCTTAT TCCGTGGTGA ACAACAACAG GGAAGGCGCA 
ATGTTGTACA TGTCAGCGGG CGAAGTCAAC CCGATTGTGA CCATTGACAA GAACCAGTTT 
AGATACAATT GTGAGAAGCT GTACGGGAAT TTCACGACTT GTGATGCCGC CGTGGAGATC 
GATATACAAA ACACCCAGAC TATTTTTTTC AGAAATAATT TGGTTGAAGG TAACCAAGGG 
GGGTTGGCGA TCAAGGCGGA TTCGCGCGGC TCAGCCACCT CTCTCAAGGG TTACATCCAC 
AATAATTTGT TTGTGAATAA TAGCAACTTG CCGGTGTTGT ACGTGGAAGG ACGGCAGTCG 
TCCCCCTATC AAGAAGTGGT GATTTACCGA AACTACTTTA CGAGGAACAA AGCACAATAC 
TACAATAATA TTGTTCTGAA ACAGGTCGTT TCCAACTTTA CGCTAAATTA CGTGAAGAGG 
AATGTCGGTT TGAAAAATTT GGAAGTGTCA GGGTTTGATA AAGTCAGGCT TCCGATTTAC 
CAAACGACGT CTCATAATGG GTTTTATCAT AATTATGCCA TTCATCGCGA TAGCAGATCG 
ACGATTGTGG CGGGGACTGC AGGTCAACAT TACGTGGATA ATATCTTCTT TAACCCAGAT 
AATGATTATG AAATGATAAC AGTAAACAGA TCTCTATTTG AATTCAACAG CACTTTACAA 
TTATGGAACA CAAAAATCGA CGCTGCTTAC AACTACTGGG GCCTTAACAA CTCTCTGGGT 
GTCCGAGGTC GCATCAAGGA CCAAAGCGAC GATCCTCGTC TCCTTGAAGT TATTTACGAG 
CCTTATCACA TGAACAATAA ATCAGTTTTA CTCAATAAAA AATGTCCCCC GGGATGGGAT 
CTCGTCGGAG GGACTTGTTA TATTTATATT GGTGCTCCCA TGACCTTCCA TGAAGCTCGA 
GCTTTTTGTC AGGCTGATAA CGCAACAATG CCGTATTTGT TAGGCAATTT GAATTACATC 
GAACTGTATG AGTTTTTACG ATCGCAACAA CAGTGGTTTT TGTACAATGA TAGGATTTGG 
GTGCAACATA TCGACAAAAT CAACGAGTGC ACCATGTTTG CCTACCAAAG GGTCGAGGTT 
GATAACTGCG ATCAAAGGAG TCCCTTCATC TGCGAAATAG ATCCTAAAGT TTCGATAAAC 
ATCCTGCCAT TGACCGACGA TGTTGTTACA ATAGCCGTTC TGTCAAGCCT GGCTTTGGCC 
TTACTTTTGA TGGCCATAGT CGGTGTTTTT TGGTGGAGCA AATCAAAGTA TCGCCAAACC 
CAAAGGCTGG AAAGACGGAA CAGTATCAGG CAAAGTTTAC ATTCATTGCG CTCCGTTGGC 
CTGGCACCTG GCAGTTTCTC TGACGCTAGT TACCGCCGAA AAGTGGCACA ACTTAGCTCG 
CGCTCAACCG ACACACTAAC AAAAAATTCC GAATACAAGA AAATGATAAC GAATGGATCA 
CTAGACAGTA TGGAAAAATC CACCTACACG TCGTCCCTTG ACGACAACCA GTCCCCTACA 
ATTGAGTATC ACAAATCACC AAATAGGTTC GATGCCCAGT ATGCCAAACC TAACGGGTTC 
GAACTGGCGT ACAAAAACGA GGGTTTTCGC GATAATTCGA CTTTTGCAAC GAATTCAAAT 
TATCAATCAC GGGCCGAAAG CATCCAAGAC AGTACCAACG ACGACATCCC GATTATCCAA 
GACAACGTTG GCGGGAACAG TGTTTCCTAC CCCCCTAGTG AATACTACAC CACTGACACG 
CTCCCCCTGA GCGGAAAATC GGACTCAACC CTTGACCTGA AGAAGGGAAT TGATGATTAT 
AATAAAAACC GCAACTACGA CCCGATCTAC AGTCGCGAAA GACCAAATGC GAATTTGTTG 
AACGAGTTGA AAAATAAATT CCCGCAAACG CCGCCGCCGC CACCAGACCC GGTCCAATCG 
CCCACTTATT CGGAAAAGTT AGACAGTCTG CAATACTCCC CTGATTATAA CACGGTGGCG 
TTAGCTGAGC CCTTCTCTGC CAATATACTT GAGACCGACT TTGACGAGCC GCACCCACGG 
CCGAAAGTTC GGGCCAAAAG TGAGGTGCTG CTAGAGACTA ACTTTGACTA CACTCCGCCT 
AGTGGTAGTC CGCAGTTGAA TCACCCAATC ACCGAATATA ATAGAAGTAA GAGTCAACCG 
TTGGAGACAG CGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC657327 cDNA ORF clone, Tribolium castaneum(red flour beetle)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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