LOC657022 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC657022
Entrez Gene ID	657022
Full Name	centromere-associated protein E
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015978760.1	XP_015834246.1	centromere-associated protein E isoform X3
XM_015978756.1	XP_015834242.1	centromere-associated protein E isoform X2
XM_008195176.2	XP_008193398.1	centromere-associated protein E isoform X4
XM_015978753.1	XP_015834239.1	centromere-associated protein E isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC657022 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC657022 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTg00283	XM_008195176.2 Latest version!	Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16360	XM_015978753.1 Latest version!	Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16361	XM_015978756.1 Latest version!	Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTg16362	XM_015978760.1 Latest version!	Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC657022 ( XM_008195176.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008193398.1 Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X4, mRNA.

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CloneID	OTg00283
Clone ID Related Accession (Same CDS sequence)	XM_008195176.2
Accession Version	XM_008195176.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	centromere-associated protein E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008195176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA TGTACCCCCT TGCCAAGGGA GACCCCTTGT GTCCCCTAGG GTTCCACCCC 
CAAGTCAGGT GGCCCACTAG GTGTAAACGG TGCTTCAGAG ACTATAAGGA ACATGGGGGG 
AAGCGAAGGG ACGAAGAACC GTCCCTCCGA AGAGACGACA CCACGGCGTC GACACCTAGT 
CTGTCTTGGA GTTCAAGGGA TGCAGATAGT GAGAGAAGAC GTAGCTGGGT ATCTAGCAGC 
AACTTAGCGT CTGACCGTGA CAATAATTCT GTAAAAAGTG AAAGTAATTA TAGCAATCCA 
TCATCTTGGA CGTCCACTCC AGATTTAGCA AAATTGGAAG ACGATACTCA AGCTGTCACA 
ACAGTTAGCA TAAAATTACC AAAGAGAAAA CAAAAACCGA TCGATACGGG ACAAAGAACT 
GTTTCTGATA GTTTCTCCAT TAAACCCGAA AAGTCAGTAT TTAGTAAAAA CGATAGTTTA 
GCAGCACGCG CTAGGAAATT ACAGCAAATC AAGGAAGCAG CGGAGGAAAA ACACAAACGA 
ATCCAAATAA AAGAAGTCAA AACAATATCA GAGGAACCAA CAAATCACGA TGTACAATTT 
CTCATCCAAG TGAAACGAAA ACCTACTGTA GTGGAAGACC ATCATTCAGA GCCCGAAGAC 
GACGCTGTAA GTATAGCCGG AACCGAGACA ACTGACACCA CTTTGGTGGA TGCGCACGAT 
CACGAGTTAA GAGAACAGTT AGAGAGTTTA AAAAAAGAGC TAGAAACGAC GAAAACAAAA 
TGTGAAAGAC TTGAACGCGA AAAGAGTGAC ATTCTCCTGC GAAGACTCGC AGCGATGGAA 
ACAACAACCA GCAAGACCAC TGCTTCGGAA GTCCTGAAAC TCCAGCAAAA GTGTAACGAG 
TTGCAACAAA CTCTCGAGGA TTTCCGGGAC GAGAAGAAGA GTCTAACTTT TAAGGTGAAG 
GAGCTGGAGG AGGAGTTGGA ACAGAGACCT ACGGCCCAAG CGGCGCAGAA AATCGCCGAT 
GAGCTGCGTT CGAAGCTGCT CGCGGCTGAA ACACTGTGCG AGGAGCTGAT GGACGAGAAC 
GAAGACATCA AGAAGGAGCT GAGGGACATG GAGGAGCAAA TGGACGAAAT GCAGGACAAC 
TTCCGCGAAG ACCAGGCCGT GGAATACACC TCACTCAAAA AAGAGTTGGA TCAGACCACC 
AAAAACTGCA GGATTTTGTC GTTCAAGCTG CGAAAAGCCG AACGCAAAAC TGAACAGTTG 
GAGCAGGAAA AGAACGAAGC GGAGCGGAAG TTGAAAGAGA TCTCAGGGGG ACAGTCAACC 
TTGGCCAATT TGGAGAAAAT GAAACAACTC GAACAGGATT TGAAGCTAGC TAACGAAGTC 
TCGATTCGGT TGCAAAAGGA GTTGGATGAG ACGAATCAAA AGCTTCAAGA GGAGTCCAAG 
ACGAGCAAAA AGAAAGCGCC GAAGTTGGGA TCCATCGGCA AATTCCCATC TGCTGATGGA 
AAAGTTTCCC GCGAGTCTCT GACTCGGGGC GGTTCCCAAG ACGATCCCGC TCAGTTGCTG 
AGAGACCTCC AAGATTCAAT GGAACGCGAA GCTGACTTAC GGGAACAACT GAAATTTGCT 
GAAGAAGAGG CTCAAAATTT ACGCAAGAAA GTGTCACGGA TAGAAGATGA TAATGAATCT 
TTGGTTTTGC AGCTGAAGAA AATGGCTACT CGAGCCAGAA GTCGTAAACT AAGTCCCACC 
AATAATTCAA GATTGACCCC AGAACCACCT TCTGAGAAAG GTGACGTATC AGACGACGAA 
GACCCCGCAG AAATCAAATT ACAGTTAGAG TTGAGCGAAC AAGAAGCTTC TGTTCTTCGA 
CGCAAAGTTG AAGATTTAGA AGCGGAAAAC CATAGAATTA AAACCAAAAA CAAGGAACTG 
CAAGACAAAC TTACCGCGAA AACTACAACA AAACGCACGG CTGTTGGTGG AGAAAAGGGC 
ACGACACTGC AAAATCAGAA GCTGAAAGTT CTAGAAGACG AGGCGAACGA CCTTCGGAAA 
AAACTCATCG AGAAGGAAAG AGACTGTGAA AGACTACACG CGGAACTTAG TCTAAACCAA 
AAGAGAAGTA AAAGTGTTCA AAAAAGCAAG TCTTTGGATT TGGATCAGCA AACTTTGGAC 
TTGAAACGTC AACTTCAAGT CATTGAACAA GAAGCGAGCA TTTTACGAAA TAAAATCCAA 
TCGCTTGAGG CAGAGAACGA GAAGTTAATA AGCGAGAATA AAAAGCTTCA ACTAGTCCGA 
GGAGCGAAAA ACCTCAAATC TGCGGACAAA AACTTAGACA AATACATTGA CCAAATAGCT 
TCGTTAGAAA TTGAAATTTC CGAGAAAAAC GACAAGATCA AAGCGCTGGA AGAAAAATTA 
GAAACGGCCC TTACGCAAGA TTCATCAAGT CTTAAAGGAG GAAATTACAA ACGATTTGTA 
GAGAGAACAC CTAAGAAGGT TTCACAGTTA ACATCGAAGG ATCAGTTGAA AACTATGGTG 
CATGATTTGG AGAATGAAAT TGGTGAGATG ATTGTGGCAA TAAAAACCAG CGAAAACGAG 
AAGATTAAAC TAGAGGAAGA AATGAAGAAA ATGCGACACC AGAACGAAGT CAATAAAGCG 
ATGCAAGAAC TCGAAGAAAT GAACAAGAAA TTTGAAGAGA TGAAGACTGA ATTATCGAAG 
GAAAAGGAGA AAGTGACTGA AGAGAAAAGT AAATACGACG AACTTAACAA ATCTTTGGTC 
AAAACGAAGG AATCCTTAAC GAAATCGAAT CAAGAGAAGA AGAAACTTAA AGAACAAATC 
GAGAAATCCA AGGAAGAACA GAAGAAAGTA CAAGAAGAAA AGGATAAACT GGACGAAGAA 
ATAGCTAAAT TGAAAGCTAA TTTAAAAACT GCGACTTATA AACAAGATGA ATTGACCTTA 
ATAAGCCAGA AGGCTGAGTC GCTAAAATTA GACCTTGACT CTAAAGAAAA AGAGTTAAAA 
ACCATCAAAA AAGAACTTGA TAGTAAAATC AATGAGTTAT CTGAAAAAGC TTCGAAGGTG 
TCTCAGTTAG AACGGAAATT TAGCGAAACT GAAGAAAAAT TAAAAATTGC AGAAAAACGT 
GAAAAGGATT TGGAAGCTAA AATTGAGGAA GAGAAGAGTA AAACCAAGTC GAAAGAAGGA 
GAGCAAAGTA AATGGAACGA AGAGCGCAAA AAGTACAATA ACCAAATTGA GGAATTAAAT 
AACAAAATTT TAAGTCTGGA AACCACAGTT GAGAGCAAAA AGAAATTAAT CGAGCGATTG 
GAAGAGAATT TGAAAAAAGA GCGAGAATCG TTTTCCAAAG TTGATGAACT GGAAACTCGC 
GAAATCACAA AATTAAAAGA CGAATTAAGT AAGAGCAAAG CTAATTTGGC CGACGTTGAG 
TCCAAACTGG CATCGTCCCA AAAGAGCCAA AAGAATCTCG AAGATAAGTT GAAAAAATCG 
GAGACGGACA GTAAGAATGA TAAACTGTCT CTTGAGAAGA AGAAAGGAGA GTTAGAAATT 
GAATTACAAA ACGAAAAGAA GAAAATCGAG GTGATGAAAG GCAACCACGA GAAAGAGAAC 
AAAAACAAAG AAATGGAGCT CGCTTCGCTG AAAAGCAAGA TAAAATCACT GGAGTTAAAC 
GCCGGAGCTG GGACGAAAAG ACTAGCAGAA ATCAAACAAT TCCAAGAGAC TATAGACAAA 
TTAGAAACTA ATCTAAATAA AGAAAAGCAG AAATATGAAG ACCTCACGGC TAAGTATGAA 
ATTTTGGAAG AAGAACATGT AGTAACCAAG GCAAAGTTGG TTATGGAGAA AGAAACTATC 
GAAAATCAAC TTTCTAGTAC AAGGTCACAA CTGGACGAAT TGGAAGTTGA GTTAAAAACA 
CTGAGAGAAA CGTACAACAA ACAACACGAT GAATGGATAA AGGAGAAACT GAGCATGCAA 
GAAAAAATTA AAGAGATTGA GAAACGTAAC GGAAACGGAA GCGAATTAGA TAAAGTGCGA 
TATAAAGCAA TGCTGGACGA TAAGCAATCC GAGTTGGACC AGCTGAGGAA GGAAAATGAG 
GCGATGCACG ACCAAATGGA ATACATGAGG AAGGAGACTG ACGAATTGAG GAAAAAACTG 
GACGATTACG AAAAAGTGAA CAAAGTACAA AGGAACATAA GTGCGGATAG TTCGGCAATG 
GAGAAGGAAA TAAGGCAACT TAAAGCAAAG TTGAGTTCTA TTGAAAAATC CAAAAAGCTC 
GAATTGGGCG AGTACAAAAT GCGATACGAC AACCAGCTTT CTATAGTCAA CGGCGAACTG 
CAACAGCTCC AAGGCCAAGT GATGCGGTTC AAGCGCGAAC GAGACACGTA CAAGCACATG 
CTAGAGTCGG CTCAGAAAAC TATCGGCGAT TTGAAAAATT CTCCAAGAAG TGCCAAAGAC 
AATACGAATC CTTCTGCACA CTACGATGAG TTGGAGGAAT CGAAAACAAA AATTGCAACT 
CTTGAACAAC AAATCAGTTG CATGGAGGAC GAACTTTCGG AAGCTCGCCT TGAATGTTCT 
CGACTGAAAA CCGAACTGGT GTCGGAAAGG TCCACATGGG AAGTGAAAAT GTCTGAACTG 
CATTCCCGGG TTAATGAACT AGAAGAGGAG AAAATCCTTA GTAGCGGAAG AACGAAAATT 
GTAGGACTGC GGACTCGAAT GGAACTAGCT TGGCAGAAAG AACGCGAGGA ACAGCAGAGA 
TTATTGCAGG AAACTGCGAC TTTGGCCCGA GATCTTCGGC AGACGTTGTT CGAAGTTGAG 
AGGGAACGAG ACAAGGAACG ATTAGAAGCG AAACGCAAAC AAGAACAGTT CAAAAAGTCC 
TCCGAGGAGG ATCAAGACGA AAATAAGAAG AAAATAACCG AGCTACAGTG CGATTTGCTG 
GAACTAAGAG ACGCTCACGC CAAATTGCGA ACCACAAACG AGAAGCTTCG CCGCGAAAAA 
GAACGCTACG AGAAAGAAAG AGAAGAATTC AAGTCGGTTA TTCAAGGCAA GAAGCGCCTG 
AACCAAGAAG ACGAACGAAA AGTCAACGTC CTGATTGAAC AAATCGACTA TTTGAAGCAA 
CTAGCACCAG AACTGTTCTT CTCCCGCGAT AAGGAAGCCT CATACACTCC AACTCCCCCA 
CGGAGGAGCA GAAGCTCGAA ATCGCGCGAG ACTTCACCAG CCATCGAGCG CCGCGAGTCG 
TCTTTATCAC CAGAAGACAA GCAACAAGAA ATCCAAAACA TGATGCTTAA ACTGGCAAAC 
ACTGCGGAAG ACCTGCGCCG ACTCCAGAAA CTAACCGAAG ATGAATACGA ACGGGAGAGA 
ATCAAGCGCA GTATGGGGAT GAGAAGAGCG ACGTCCACTG AACACGAAAA TGTCTCCGAA 
AGCAGATACA AGTCGTACCA GAAACGCAAA ACCGACGGGA GCTTACACCG AAAAAGCTTG 
TCTCTAGAGC ACACCATAGC CCAGAACGAC CCCAAAATCT GGAAAAACGA CAACGACAGC 
ATGTCCAGTC TCCACTCTCT GGACATGTCT TCAGACGTCG AAAGGTGGAC AACGAGGGAC 
ACGAGTCTAG ACAGTCGCCT CTCTGGTGGT TCCACCCAGA GCGAGTTCGC CGGCGAGAAG 
AAAAAGAAGA AGGGAATCAT GGGCAAGTTG AAGAAATTGA CCAAATCTCG AAGCATCGAT 
GACGAAGATC CTGGGACTTT CTCGCCCTCA AGATCACTAT CGTCAAAGCA AAATTCGAGT 
TCCGAACTTC TGGAGGAGAA AGGGAGCAAG AAGGATTTGA GGGAGCGAAT CACGGGTATT 
TTCAAGAAAA GCGGCTCGTC ATCACGGAGC AACAGTGTGG AAAGACACGA AGTGAGACAC 
GACACAAGTT CGACACACAG GCCCTTGATG CGGAATGGCA GTGGCGGGAA TTTGTCCTCA 
CGAGCGTCTC CAGCTTTTCA GGAACCAGAA AAGACACCGA ACGCAAAAGC TAAGAGAAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008193398.1
CDS	455..6457
Translation	MHHMYPLAKGDPLCPLGFHPQVRWPTRCKRCFRDYKEHGGKRRDEEPSLRRDDTTASTPSLSWSSRDADSERRRSWVSSSNLASDRDNNSVKSESNYSNPSSWTSTPDLAKLEDDTQAVTTVSIKLPKRKQKPIDTGQRTVSDSFSIKPEKSVFSKNDSLAARARKLQQIKEAAEEKHKRIQIKEVKTISEEPTNHDVQFLIQVKRKPTVVEDHHSEPEDDAVSIAGTETTDTTLVDAHDHELREQLESLKKELETTKTKCERLEREKSDILLRRLAAMETTTSKTTASEVLKLQQKCNELQQTLEDFRDEKKSLTFKVKELEEELEQRPTAQAAQKIADELRSKLLAAETLCEELMDENEDIKKELRDMEEQMDEMQDNFREDQAVEYTSLKKELDQTTKNCRILSFKLRKAERKTEQLEQEKNEAERKLKEISGGQSTLANLEKMKQLEQDLKLANEVSIRLQKELDETNQKLQEESKTSKKKAPKLGSIGKFPSADGKVSRESLTRGGSQDDPAQLLRDLQDSMEREADLREQLKFAEEEAQNLRKKVSRIEDDNESLVLQLKKMATRARSRKLSPTNNSRLTPEPPSEKGDVSDDEDPAEIKLQLELSEQEASVLRRKVEDLEAENHRIKTKNKELQDKLTAKTTTKRTAVGGEKGTTLQNQKLKVLEDEANDLRKKLIEKERDCERLHAELSLNQKRSKSVQKSKSLDLDQQTLDLKRQLQVIEQEASILRNKIQSLEAENEKLISENKKLQLVRGAKNLKSADKNLDKYIDQIASLEIEISEKNDKIKALEEKLETALTQDSSSLKGGNYKRFVERTPKKVSQLTSKDQLKTMVHDLENEIGEMIVAIKTSENEKIKLEEEMKKMRHQNEVNKAMQELEEMNKKFEEMKTELSKEKEKVTEEKSKYDELNKSLVKTKESLTKSNQEKKKLKEQIEKSKEEQKKVQEEKDKLDEEIAKLKANLKTATYKQDELTLISQKAESLKLDLDSKEKELKTIKKELDSKINELSEKASKVSQLERKFSETEEKLKIAEKREKDLEAKIEEEKSKTKSKEGEQSKWNEERKKYNNQIEELNNKILSLETTVESKKKLIERLEENLKKERESFSKVDELETREITKLKDELSKSKANLADVESKLASSQKSQKNLEDKLKKSETDSKNDKLSLEKKKGELEIELQNEKKKIEVMKGNHEKENKNKEMELASLKSKIKSLELNAGAGTKRLAEIKQFQETIDKLETNLNKEKQKYEDLTAKYEILEEEHVVTKAKLVMEKETIENQLSSTRSQLDELEVELKTLRETYNKQHDEWIKEKLSMQEKIKEIEKRNGNGSELDKVRYKAMLDDKQSELDQLRKENEAMHDQMEYMRKETDELRKKLDDYEKVNKVQRNISADSSAMEKEIRQLKAKLSSIEKSKKLELGEYKMRYDNQLSIVNGELQQLQGQVMRFKRERDTYKHMLESAQKTIGDLKNSPRSAKDNTNPSAHYDELEESKTKIATLEQQISCMEDELSEARLECSRLKTELVSERSTWEVKMSELHSRVNELEEEKILSSGRTKIVGLRTRMELAWQKEREEQQRLLQETATLARDLRQTLFEVERERDKERLEAKRKQEQFKKSSEEDQDENKKKITELQCDLLELRDAHAKLRTTNEKLRREKERYEKEREEFKSVIQGKKRLNQEDERKVNVLIEQIDYLKQLAPELFFSRDKEASYTPTPPRRSRSSKSRETSPAIERRESSLSPEDKQQEIQNMMLKLANTAEDLRRLQKLTEDEYERERIKRSMGMRRATSTEHENVSESRYKSYQKRKTDGSLHRKSLSLEHTIAQNDPKIWKNDNDSMSSLHSLDMSSDVERWTTRDTSLDSRLSGGSTQSEFAGEKKKKKGIMGKLKKLTKSRSIDDEDPGTFSPSRSLSSKQNSSSELLEEKGSKKDLRERITGIFKKSGSSSRSNSVERHEVRHDTSSTHRPLMRNGSGGNLSSRASPAFQEPEKTPNAKAKRN

Target ORF information:

RefSeq Version	XM_008195176.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008195176.2

ORF Insert Sequence:

ATGCATCACA TGTACCCCCT TGCCAAGGGA GACCCCTTGT GTCCCCTAGG GTTCCACCCC 
CAAGTCAGGT GGCCCACTAG GTGTAAACGG TGCTTCAGAG ACTATAAGGA ACATGGGGGG 
AAGCGAAGGG ACGAAGAACC GTCCCTCCGA AGAGACGACA CCACGGCGTC GACACCTAGT 
CTGTCTTGGA GTTCAAGGGA TGCAGATAGT GAGAGAAGAC GTAGCTGGGT ATCTAGCAGC 
AACTTAGCGT CTGACCGTGA CAATAATTCT GTAAAAAGTG AAAGTAATTA TAGCAATCCA 
TCATCTTGGA CGTCCACTCC AGATTTAGCA AAATTGGAAG ACGATACTCA AGCTGTCACA 
ACAGTTAGCA TAAAATTACC AAAGAGAAAA CAAAAACCGA TCGATACGGG ACAAAGAACT 
GTTTCTGATA GTTTCTCCAT TAAACCCGAA AAGTCAGTAT TTAGTAAAAA CGATAGTTTA 
GCAGCACGCG CTAGGAAATT ACAGCAAATC AAGGAAGCAG CGGAGGAAAA ACACAAACGA 
ATCCAAATAA AAGAAGTCAA AACAATATCA GAGGAACCAA CAAATCACGA TGTACAATTT 
CTCATCCAAG TGAAACGAAA ACCTACTGTA GTGGAAGACC ATCATTCAGA GCCCGAAGAC 
GACGCTGTAA GTATAGCCGG AACCGAGACA ACTGACACCA CTTTGGTGGA TGCGCACGAT 
CACGAGTTAA GAGAACAGTT AGAGAGTTTA AAAAAAGAGC TAGAAACGAC GAAAACAAAA 
TGTGAAAGAC TTGAACGCGA AAAGAGTGAC ATTCTCCTGC GAAGACTCGC AGCGATGGAA 
ACAACAACCA GCAAGACCAC TGCTTCGGAA GTCCTGAAAC TCCAGCAAAA GTGTAACGAG 
TTGCAACAAA CTCTCGAGGA TTTCCGGGAC GAGAAGAAGA GTCTAACTTT TAAGGTGAAG 
GAGCTGGAGG AGGAGTTGGA ACAGAGACCT ACGGCCCAAG CGGCGCAGAA AATCGCCGAT 
GAGCTGCGTT CGAAGCTGCT CGCGGCTGAA ACACTGTGCG AGGAGCTGAT GGACGAGAAC 
GAAGACATCA AGAAGGAGCT GAGGGACATG GAGGAGCAAA TGGACGAAAT GCAGGACAAC 
TTCCGCGAAG ACCAGGCCGT GGAATACACC TCACTCAAAA AAGAGTTGGA TCAGACCACC 
AAAAACTGCA GGATTTTGTC GTTCAAGCTG CGAAAAGCCG AACGCAAAAC TGAACAGTTG 
GAGCAGGAAA AGAACGAAGC GGAGCGGAAG TTGAAAGAGA TCTCAGGGGG ACAGTCAACC 
TTGGCCAATT TGGAGAAAAT GAAACAACTC GAACAGGATT TGAAGCTAGC TAACGAAGTC 
TCGATTCGGT TGCAAAAGGA GTTGGATGAG ACGAATCAAA AGCTTCAAGA GGAGTCCAAG 
ACGAGCAAAA AGAAAGCGCC GAAGTTGGGA TCCATCGGCA AATTCCCATC TGCTGATGGA 
AAAGTTTCCC GCGAGTCTCT GACTCGGGGC GGTTCCCAAG ACGATCCCGC TCAGTTGCTG 
AGAGACCTCC AAGATTCAAT GGAACGCGAA GCTGACTTAC GGGAACAACT GAAATTTGCT 
GAAGAAGAGG CTCAAAATTT ACGCAAGAAA GTGTCACGGA TAGAAGATGA TAATGAATCT 
TTGGTTTTGC AGCTGAAGAA AATGGCTACT CGAGCCAGAA GTCGTAAACT AAGTCCCACC 
AATAATTCAA GATTGACCCC AGAACCACCT TCTGAGAAAG GTGACGTATC AGACGACGAA 
GACCCCGCAG AAATCAAATT ACAGTTAGAG TTGAGCGAAC AAGAAGCTTC TGTTCTTCGA 
CGCAAAGTTG AAGATTTAGA AGCGGAAAAC CATAGAATTA AAACCAAAAA CAAGGAACTG 
CAAGACAAAC TTACCGCGAA AACTACAACA AAACGCACGG CTGTTGGTGG AGAAAAGGGC 
ACGACACTGC AAAATCAGAA GCTGAAAGTT CTAGAAGACG AGGCGAACGA CCTTCGGAAA 
AAACTCATCG AGAAGGAAAG AGACTGTGAA AGACTACACG CGGAACTTAG TCTAAACCAA 
AAGAGAAGTA AAAGTGTTCA AAAAAGCAAG TCTTTGGATT TGGATCAGCA AACTTTGGAC 
TTGAAACGTC AACTTCAAGT CATTGAACAA GAAGCGAGCA TTTTACGAAA TAAAATCCAA 
TCGCTTGAGG CAGAGAACGA GAAGTTAATA AGCGAGAATA AAAAGCTTCA ACTAGTCCGA 
GGAGCGAAAA ACCTCAAATC TGCGGACAAA AACTTAGACA AATACATTGA CCAAATAGCT 
TCGTTAGAAA TTGAAATTTC CGAGAAAAAC GACAAGATCA AAGCGCTGGA AGAAAAATTA 
GAAACGGCCC TTACGCAAGA TTCATCAAGT CTTAAAGGAG GAAATTACAA ACGATTTGTA 
GAGAGAACAC CTAAGAAGGT TTCACAGTTA ACATCGAAGG ATCAGTTGAA AACTATGGTG 
CATGATTTGG AGAATGAAAT TGGTGAGATG ATTGTGGCAA TAAAAACCAG CGAAAACGAG 
AAGATTAAAC TAGAGGAAGA AATGAAGAAA ATGCGACACC AGAACGAAGT CAATAAAGCG 
ATGCAAGAAC TCGAAGAAAT GAACAAGAAA TTTGAAGAGA TGAAGACTGA ATTATCGAAG 
GAAAAGGAGA AAGTGACTGA AGAGAAAAGT AAATACGACG AACTTAACAA ATCTTTGGTC 
AAAACGAAGG AATCCTTAAC GAAATCGAAT CAAGAGAAGA AGAAACTTAA AGAACAAATC 
GAGAAATCCA AGGAAGAACA GAAGAAAGTA CAAGAAGAAA AGGATAAACT GGACGAAGAA 
ATAGCTAAAT TGAAAGCTAA TTTAAAAACT GCGACTTATA AACAAGATGA ATTGACCTTA 
ATAAGCCAGA AGGCTGAGTC GCTAAAATTA GACCTTGACT CTAAAGAAAA AGAGTTAAAA 
ACCATCAAAA AAGAACTTGA TAGTAAAATC AATGAGTTAT CTGAAAAAGC TTCGAAGGTG 
TCTCAGTTAG AACGGAAATT TAGCGAAACT GAAGAAAAAT TAAAAATTGC AGAAAAACGT 
GAAAAGGATT TGGAAGCTAA AATTGAGGAA GAGAAGAGTA AAACCAAGTC GAAAGAAGGA 
GAGCAAAGTA AATGGAACGA AGAGCGCAAA AAGTACAATA ACCAAATTGA GGAATTAAAT 
AACAAAATTT TAAGTCTGGA AACCACAGTT GAGAGCAAAA AGAAATTAAT CGAGCGATTG 
GAAGAGAATT TGAAAAAAGA GCGAGAATCG TTTTCCAAAG TTGATGAACT GGAAACTCGC 
GAAATCACAA AATTAAAAGA CGAATTAAGT AAGAGCAAAG CTAATTTGGC CGACGTTGAG 
TCCAAACTGG CATCGTCCCA AAAGAGCCAA AAGAATCTCG AAGATAAGTT GAAAAAATCG 
GAGACGGACA GTAAGAATGA TAAACTGTCT CTTGAGAAGA AGAAAGGAGA GTTAGAAATT 
GAATTACAAA ACGAAAAGAA GAAAATCGAG GTGATGAAAG GCAACCACGA GAAAGAGAAC 
AAAAACAAAG AAATGGAGCT CGCTTCGCTG AAAAGCAAGA TAAAATCACT GGAGTTAAAC 
GCCGGAGCTG GGACGAAAAG ACTAGCAGAA ATCAAACAAT TCCAAGAGAC TATAGACAAA 
TTAGAAACTA ATCTAAATAA AGAAAAGCAG AAATATGAAG ACCTCACGGC TAAGTATGAA 
ATTTTGGAAG AAGAACATGT AGTAACCAAG GCAAAGTTGG TTATGGAGAA AGAAACTATC 
GAAAATCAAC TTTCTAGTAC AAGGTCACAA CTGGACGAAT TGGAAGTTGA GTTAAAAACA 
CTGAGAGAAA CGTACAACAA ACAACACGAT GAATGGATAA AGGAGAAACT GAGCATGCAA 
GAAAAAATTA AAGAGATTGA GAAACGTAAC GGAAACGGAA GCGAATTAGA TAAAGTGCGA 
TATAAAGCAA TGCTGGACGA TAAGCAATCC GAGTTGGACC AGCTGAGGAA GGAAAATGAG 
GCGATGCACG ACCAAATGGA ATACATGAGG AAGGAGACTG ACGAATTGAG GAAAAAACTG 
GACGATTACG AAAAAGTGAA CAAAGTACAA AGGAACATAA GTGCGGATAG TTCGGCAATG 
GAGAAGGAAA TAAGGCAACT TAAAGCAAAG TTGAGTTCTA TTGAAAAATC CAAAAAGCTC 
GAATTGGGCG AGTACAAAAT GCGATACGAC AACCAGCTTT CTATAGTCAA CGGCGAACTG 
CAACAGCTCC AAGGCCAAGT GATGCGGTTC AAGCGCGAAC GAGACACGTA CAAGCACATG 
CTAGAGTCGG CTCAGAAAAC TATCGGCGAT TTGAAAAATT CTCCAAGAAG TGCCAAAGAC 
AATACGAATC CTTCTGCACA CTACGATGAG TTGGAGGAAT CGAAAACAAA AATTGCAACT 
CTTGAACAAC AAATCAGTTG CATGGAGGAC GAACTTTCGG AAGCTCGCCT TGAATGTTCT 
CGACTGAAAA CCGAACTGGT GTCGGAAAGG TCCACATGGG AAGTGAAAAT GTCTGAACTG 
CATTCCCGGG TTAATGAACT AGAAGAGGAG AAAATCCTTA GTAGCGGAAG AACGAAAATT 
GTAGGACTGC GGACTCGAAT GGAACTAGCT TGGCAGAAAG AACGCGAGGA ACAGCAGAGA 
TTATTGCAGG AAACTGCGAC TTTGGCCCGA GATCTTCGGC AGACGTTGTT CGAAGTTGAG 
AGGGAACGAG ACAAGGAACG ATTAGAAGCG AAACGCAAAC AAGAACAGTT CAAAAAGTCC 
TCCGAGGAGG ATCAAGACGA AAATAAGAAG AAAATAACCG AGCTACAGTG CGATTTGCTG 
GAACTAAGAG ACGCTCACGC CAAATTGCGA ACCACAAACG AGAAGCTTCG CCGCGAAAAA 
GAACGCTACG AGAAAGAAAG AGAAGAATTC AAGTCGGTTA TTCAAGGCAA GAAGCGCCTG 
AACCAAGAAG ACGAACGAAA AGTCAACGTC CTGATTGAAC AAATCGACTA TTTGAAGCAA 
CTAGCACCAG AACTGTTCTT CTCCCGCGAT AAGGAAGCCT CATACACTCC AACTCCCCCA 
CGGAGGAGCA GAAGCTCGAA ATCGCGCGAG ACTTCACCAG CCATCGAGCG CCGCGAGTCG 
TCTTTATCAC CAGAAGACAA GCAACAAGAA ATCCAAAACA TGATGCTTAA ACTGGCAAAC 
ACTGCGGAAG ACCTGCGCCG ACTCCAGAAA CTAACCGAAG ATGAATACGA ACGGGAGAGA 
ATCAAGCGCA GTATGGGGAT GAGAAGAGCG ACGTCCACTG AACACGAAAA TGTCTCCGAA 
AGCAGATACA AGTCGTACCA GAAACGCAAA ACCGACGGGA GCTTACACCG AAAAAGCTTG 
TCTCTAGAGC ACACCATAGC CCAGAACGAC CCCAAAATCT GGAAAAACGA CAACGACAGC 
ATGTCCAGTC TCCACTCTCT GGACATGTCT TCAGACGTCG AAAGGTGGAC AACGAGGGAC 
ACGAGTCTAG ACAGTCGCCT CTCTGGTGGT TCCACCCAGA GCGAGTTCGC CGGCGAGAAG 
AAAAAGAAGA AGGGAATCAT GGGCAAGTTG AAGAAATTGA CCAAATCTCG AAGCATCGAT 
GACGAAGATC CTGGGACTTT CTCGCCCTCA AGATCACTAT CGTCAAAGCA AAATTCGAGT 
TCCGAACTTC TGGAGGAGAA AGGGAGCAAG AAGGATTTGA GGGAGCGAAT CACGGGTATT 
TTCAAGAAAA GCGGCTCGTC ATCACGGAGC AACAGTGTGG AAAGACACGA AGTGAGACAC 
GACACAAGTT CGACACACAG GCCCTTGATG CGGAATGGCA GTGGCGGGAA TTTGTCCTCA 
CGAGCGTCTC CAGCTTTTCA GGAACCAGAA AAGACACCGA ACGCAAAAGC TAAGAGAAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC657022 ( XM_015978753.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015834239.1 Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X1, mRNA.

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CloneID	OTg16360
Clone ID Related Accession (Same CDS sequence)	XM_015978753.1
Accession Version	XM_015978753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	centromere-associated protein E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA TGTACCCCCT TGCCAAGGGA GACCCCTTGT GTCCCCTAGG GTTCCACCCC 
CAAGTCAGGT GGCCCACTAG GTGTAAACGG TGCTTCAGAG ACTATAAGGA ACATGGGGGG 
AAGCGAAGGG ACGAAGAACC GTCCCTCCGA AGAGACGACA CCACGGCGTC GACACCTAGT 
CTGTCTTGGA GTTCAAGGGA TGCAGATAGT GAGAGAAGAC GTAGCTGGGT ATCTAGCAGC 
AACTTAGCGT CTGACCGTGA CAATAATTCT GTAAAAAGTG AAAGTAATTA TAGCAATCCA 
TCATCTTGGA CGTCCACTCC AGATTTAGCA AAATTGGAAG ACGATACTCA AGCTGTCACA 
ACAGTTAGCA TAAAATTACC AAAGAGAAAA CAAAAACCGA TCGATACGGG ACAAAGAACT 
GTTTCTGATA GTTTCTCCAT TAAACCCGAA AAGTCAGTAT TTAGTAAAAA CGATAGTTTA 
GCAGCACGCG CTAGGAAATT ACAGCAAATC AAGGAAGCAG CGGAGGAAAA ACACAAACGA 
ATCCAAATAA AAGAAGTCAA AACAATATCA GAGGAACCAA CAAATCACGA TGTACAATTT 
CTCATCCAAG TGAAACGAAA ACCTACTGTA GTGGAAGACC ATCATTCAGA GCCCGAAGAC 
GACGCTGTAA GTATAGCCGG AACCGAGACA ACTGACACCA CTTTGGTGGA TGCGCACGAT 
CACGAGTTAA GAGAACAGTT AGAGAGTTTA AAAAAAGAGC TAGAAACGAC GAAAACAAAA 
TGTGAAAGAC TTGAACGCGA AAAGAGTGAC ATTCTCCTGC GAAGACTCGC AGCGATGGAA 
ACAACAACCA GCAAGACCAC TGCTTCGGAA GTCCTGAAAC TCCAGCAAAA GTGTAACGAG 
TTGCAACAAA CTCTCGAGGA TTTCCGGGAC GAGAAGAAGA GTCTAACTTT TAAGGTGAAG 
GAGCTGGAGG AGGAGTTGGA ACAGAGACCT ACGGCCCAAG CGGCGCAGAA AATCGCCGAT 
GAGCTGCGTT CGAAGCTGCT CGCGGCTGAA ACACTGTGCG AGGAGCTGAT GGACGAGAAC 
GAAGACATCA AGAAGGAGCT GAGGGACATG GAGGAGCAAA TGGACGAAAT GCAGGACAAC 
TTCCGCGAAG ACCAGGCCGT GGAATACACC TCACTCAAAA AAGAGTTGGA TCAGACCACC 
AAAAACTGCA GGATTTTGTC GTTCAAGCTG CGAAAAGCCG AACGCAAAAC TGAACAGTTG 
GAGCAGGAAA AGAACGAAGC GGAGCGGAAG TTGAAAGAGA TCTCAGGGGG ACAGTCAACC 
TTGGCCAATT TGGAGAAAAT GAAACAACTC GAACAGGATT TGAAGCTAGC TAACGAAGTC 
TCGATTCGGT TGCAAAAGGA GTTGGATGAG ACGAATCAAA AGCTTCAAGA GGAGTCCAAG 
ACGAGCAAAA AGAAAGCGCC GAAGTTGGGA TCCATCGGCA AATTCCCATC TGCTGATGGA 
AAAGTTTCCC GCGAGTCTCT GACTCGGGGC GGTTCCCAAG ACGATCCCGC TCAGTTGCTG 
AGAGACCTCC AAGATTCAAT GGAACGCGAA GCTGACTTAC GGGAACAACT GAAATTTGCT 
GAAGAAGAGA TTTGTAAAAA ATCATGCGGA CATTTCAAAT CCCCCGAATT GCATATTTTT 
CAACGCAAAT CCCCTTTAGT ATTGTTTGAG TCTCAAAACG TGGAGACCAA ACTTTTCACA 
GTATCGACCC AAACTTTGTT AAATCTTGAC TCATCTGAAG CAGGAACACA AGCCGCATTT 
GATATCTGTG ACGCCTCTAG TCAAACAATT GAATGGCGAC CACAAAGTTT CATTTCTTCC 
ATTCAATTCC ATCATCTCAC AATGTCCAAA ATTTACCAAC CAGCGAGACA TTTCTCACCA 
ATGGCCACTT TCCTGAACGC AATGGGTCGT AAACTAAGTC CCACCAATAA TTCAAGATTG 
ACCCCAGAAC CACCTTCTGA GAAAGGTGAC GTATCAGACG ACGAAGACCC CGCAGAAATC 
AAATTACAGT TAGAGTTGAG CGAACAAGAA GCTTCTGTTC TTCGACGCAA AGTTGAAGAT 
TTAGAAGCGG AAAACCATAG AATTAAAACC AAAAACAAGG AACTGCAAGA CAAACTTACC 
GCGAAAACTA CAACAAAACG CACGGCTGTT GGTGGAGAAA AGGGCACGAC ACTGCAAAAT 
CAGAAGCTGA AAGTTCTAGA AGACGAGGCG AACGACCTTC GGAAAAAACT CATCGAGAAG 
GAAAGAGACT GTGAAAGACT ACACGCGGAA CTTAGTCTAA ACCAAAAGAG AAGTAAAAGT 
GTTCAAAAAA GCAAGTCTTT GGATTTGGAT CAGCAAACTT TGGACTTGAA ACGTCAACTT 
CAAGTCATTG AACAAGAAGC GAGCATTTTA CGAAATAAAA TCCAATCGCT TGAGGCAGAG 
AACGAGAAGT TAATAAGCGA GAATAAAAAG CTTCAACTAG TCCGAGGAGC GAAAAACCTC 
AAATCTGCGG ACAAAAACTT AGACAAATAC ATTGACCAAA TAGCTTCGTT AGAAATTGAA 
ATTTCCGAGA AAAACGACAA GATCAAAGCG CTGGAAGAAA AATTAGAAAC GGCCCTTACG 
CAAGATTCAT CAAGTCTTAA AGGAGGAAAT TACAAACGAT TTGTAGAGAG AACACCTAAG 
AAGGTTTCAC AGTTAACATC GAAGGATCAG TTGAAAACTA TGGTGCATGA TTTGGAGAAT 
GAAATTGGTG AGATGATTGT GGCAATAAAA ACCAGCGAAA ACGAGAAGAT TAAACTAGAG 
GAAGAAATGA AGAAAATGCG ACACCAGAAC GAAGTCAATA AAGCGATGCA AGAACTCGAA 
GAAATGAACA AGAAATTTGA AGAGATGAAG ACTGAATTAT CGAAGGAAAA GGAGAAAGTG 
ACTGAAGAGA AAAGTAAATA CGACGAACTT AACAAATCTT TGGTCAAAAC GAAGGAATCC 
TTAACGAAAT CGAATCAAGA GAAGAAGAAA CTTAAAGAAC AAATCGAGAA ATCCAAGGAA 
GAACAGAAGA AAGTACAAGA AGAAAAGGAT AAACTGGACG AAGAAATAGC TAAATTGAAA 
GCTAATTTAA AAACTGCGAC TTATAAACAA GATGAATTGA CCTTAATAAG CCAGAAGGCT 
GAGTCGCTAA AATTAGACCT TGACTCTAAA GAAAAAGAGT TAAAAACCAT CAAAAAAGAA 
CTTGATAGTA AAATCAATGA GTTATCTGAA AAAGCTTCGA AGGTGTCTCA GTTAGAACGG 
AAATTTAGCG AAACTGAAGA AAAATTAAAA ATTGCAGAAA AACGTGAAAA GGATTTGGAA 
GCTAAAATTG AGGAAGAGAA GAGTAAAACC AAGTCGAAAG AAGGAGAGCA AAGTAAATGG 
AACGAAGAGC GCAAAAAGTA CAATAACCAA ATTGAGGAAT TAAATAACAA AATTTTAAGT 
CTGGAAACCA CAGTTGAGAG CAAAAAGAAA TTAATCGAGC GATTGGAAGA GAATTTGAAA 
AAAGAGCGAG AATCGTTTTC CAAAGTTGAT GAACTGGAAA CTCGCGAAAT CACAAAATTA 
AAAGACGAAT TAAGTAAGAG CAAAGCTAAT TTGGCCGACG TTGAGTCCAA ACTGGCATCG 
TCCCAAAAGA GCCAAAAGAA TCTCGAAGAT AAGTTGAAAA AATCGGAGAC GGACAGTAAG 
AATGATAAAC TGTCTCTTGA GAAGAAGAAA GGAGAGTTAG AAATTGAATT ACAAAACGAA 
AAGAAGAAAA TCGAGGTGAT GAAAGGCAAC CACGAGAAAG AGAACAAAAA CAAAGAAATG 
GAGCTCGCTT CGCTGAAAAG CAAGATAAAA TCACTGGAGT TAAACGCCGG AGCTGGGACG 
AAAAGACTAG CAGAAATCAA ACAATTCCAA GAGACTATAG ACAAATTAGA AACTAATCTA 
AATAAAGAAA AGCAGAAATA TGAAGACCTC ACGGCTAAGT ATGAAATTTT GGAAGAAGAA 
CATGTAGTAA CCAAGGCAAA GTTGGTTATG GAGAAAGAAA CTATCGAAAA TCAACTTTCT 
AGTACAAGGT CACAACTGGA CGAATTGGAA GTTGAGTTAA AAACACTGAG AGAAACGTAC 
AACAAACAAC ACGATGAATG GATAAAGGAG AAACTGAGCA TGCAAGAAAA AATTAAAGAG 
ATTGAGAAAC GTAACGGAAA CGGAAGCGAA TTAGATAAAG TGCGATATAA AGCAATGCTG 
GACGATAAGC AATCCGAGTT GGACCAGCTG AGGAAGGAAA ATGAGGCGAT GCACGACCAA 
ATGGAATACA TGAGGAAGGA GACTGACGAA TTGAGGAAAA AACTGGACGA TTACGAAAAA 
GTGAACAAAG TACAAAGGAA CATAAGTGCG GATAGTTCGG CAATGGAGAA GGAAATAAGG 
CAACTTAAAG CAAAGTTGAG TTCTATTGAA AAATCCAAAA AGCTCGAATT GGGCGAGTAC 
AAAATGCGAT ACGACAACCA GCTTTCTATA GTCAACGGCG AACTGCAACA GCTCCAAGGC 
CAAGTGATGC GGTTCAAGCG CGAACGAGAC ACGTACAAGC ACATGCTAGA GTCGGCTCAG 
AAAACTATCG GCGATTTGAA AAATTCTCCA AGAAGTGCCA AAGACAATAC GAATCCTTCT 
GCACACTACG ATGAGTTGGA GGAATCGAAA ACAAAAATTG CAACTCTTGA ACAACAAATC 
AGTTGCATGG AGGACGAACT TTCGGAAGCT CGCCTTGAAT GTTCTCGACT GAAAACCGAA 
CTGGTGTCGG AAAGGTCCAC ATGGGAAGTG AAAATGTCTG AACTGCATTC CCGGGTTAAT 
GAACTAGAAG AGGAGAAAAT CCTTAGTAGC GGAAGAACGA AAATTGTAGG ACTGCGGACT 
CGAATGGAAC TAGCTTGGCA GAAAGAACGC GAGGAACAGC AGAGATTATT GCAGGAAACT 
GCGACTTTGG CCCGAGATCT TCGGCAGACG TTGTTCGAAG TTGAGAGGGA ACGAGACAAG 
GAACGATTAG AAGCGAAACG CAAACAAGAA CAGTTCAAAA AGTCCTCCGA GGAGGATCAA 
GACGAAAATA AGAAGAAAAT AACCGAGCTA CAGTGCGATT TGCTGGAACT AAGAGACGCT 
CACGCCAAAT TGCGAACCAC AAACGAGAAG CTTCGCCGCG AAAAAGAACG CTACGAGAAA 
GAAAGAGAAG AATTCAAGTC GGTTATTCAA GGCAAGAAGC GCCTGAACCA AGAAGACGAA 
CGAAAAGTCA ACGTCCTGAT TGAACAAATC GACTATTTGA AGCAACTAGC ACCAGAACTG 
TTCTTCTCCC GCGATAAGGA AGCCTCATAC ACTCCAACTC CCCCACGGAG GAGCAGAAGC 
TCGAAATCGC GCGAGACTTC ACCAGCCATC GAGCGCCGCG AGTCGTCTTT ATCACCAGAA 
GACAAGCAAC AAGAAATCCA AAACATGATG CTTAAACTGG CAAACACTGC GGAAGACCTG 
CGCCGACTCC AGAAACTAAC CGAAGATGAA TACGAACGGG AGAGAATCAA GCGCAGTATG 
GGGATGAGAA GAGCGACGTC CACTGAACAC GAAAATGTCT CCGAAAGCAG ATACAAGTCG 
TACCAGAAAC GCAAAACCGA CGGGAGCTTA CACCGAAAAA GCTTGTCTCT AGAGCACACC 
ATAGCCCAGA ACGACCCCAA AATCTGGAAA AACGACAACG ACAGCATGTC CAGTCTCCAC 
TCTCTGGACA TGTCTTCAGA CGTCGAAAGG TGGACAACGA GGGACACGAG TCTAGACAGT 
CGCCTCTCTG GTGGTTCCAC CCAGAGCGAG TTCGCCGGCG AGAAGAAAAA GAAGAAGGGA 
ATCATGGGCA AGTTGAAGAA ATTGACCAAA TCTCGAAGCA TCGATGACGA AGATCCTGGG 
ACTTTCTCGC CCTCAAGATC ACTATCGTCA AAGCAAAATT CGAGTTCCGA ACTTCTGGAG 
GAGAAAGGGA GCAAGAAGGA TTTGAGGGAG CGAATCACGG GTATTTTCAA GAAAAGCGGC 
TCGTCATCAC GGAGCAACAG TGTGGAAAGA CACGAAGTGA GACACGACAC AAGTTCGACA 
CACAGGCCCT TGATGCGGAA TGGCAGTGGC GGGAATTTGT CCTCACGAGC GTCTCCAGCT 
TTTCAGGAAC CAGAAAAGAC ACCGAACGCA AAAGCTAAGA GAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015834239.1
CDS	475..6702
Translation	MHHMYPLAKGDPLCPLGFHPQVRWPTRCKRCFRDYKEHGGKRRDEEPSLRRDDTTASTPSLSWSSRDADSERRRSWVSSSNLASDRDNNSVKSESNYSNPSSWTSTPDLAKLEDDTQAVTTVSIKLPKRKQKPIDTGQRTVSDSFSIKPEKSVFSKNDSLAARARKLQQIKEAAEEKHKRIQIKEVKTISEEPTNHDVQFLIQVKRKPTVVEDHHSEPEDDAVSIAGTETTDTTLVDAHDHELREQLESLKKELETTKTKCERLEREKSDILLRRLAAMETTTSKTTASEVLKLQQKCNELQQTLEDFRDEKKSLTFKVKELEEELEQRPTAQAAQKIADELRSKLLAAETLCEELMDENEDIKKELRDMEEQMDEMQDNFREDQAVEYTSLKKELDQTTKNCRILSFKLRKAERKTEQLEQEKNEAERKLKEISGGQSTLANLEKMKQLEQDLKLANEVSIRLQKELDETNQKLQEESKTSKKKAPKLGSIGKFPSADGKVSRESLTRGGSQDDPAQLLRDLQDSMEREADLREQLKFAEEEICKKSCGHFKSPELHIFQRKSPLVLFESQNVETKLFTVSTQTLLNLDSSEAGTQAAFDICDASSQTIEWRPQSFISSIQFHHLTMSKIYQPARHFSPMATFLNAMGRKLSPTNNSRLTPEPPSEKGDVSDDEDPAEIKLQLELSEQEASVLRRKVEDLEAENHRIKTKNKELQDKLTAKTTTKRTAVGGEKGTTLQNQKLKVLEDEANDLRKKLIEKERDCERLHAELSLNQKRSKSVQKSKSLDLDQQTLDLKRQLQVIEQEASILRNKIQSLEAENEKLISENKKLQLVRGAKNLKSADKNLDKYIDQIASLEIEISEKNDKIKALEEKLETALTQDSSSLKGGNYKRFVERTPKKVSQLTSKDQLKTMVHDLENEIGEMIVAIKTSENEKIKLEEEMKKMRHQNEVNKAMQELEEMNKKFEEMKTELSKEKEKVTEEKSKYDELNKSLVKTKESLTKSNQEKKKLKEQIEKSKEEQKKVQEEKDKLDEEIAKLKANLKTATYKQDELTLISQKAESLKLDLDSKEKELKTIKKELDSKINELSEKASKVSQLERKFSETEEKLKIAEKREKDLEAKIEEEKSKTKSKEGEQSKWNEERKKYNNQIEELNNKILSLETTVESKKKLIERLEENLKKERESFSKVDELETREITKLKDELSKSKANLADVESKLASSQKSQKNLEDKLKKSETDSKNDKLSLEKKKGELEIELQNEKKKIEVMKGNHEKENKNKEMELASLKSKIKSLELNAGAGTKRLAEIKQFQETIDKLETNLNKEKQKYEDLTAKYEILEEEHVVTKAKLVMEKETIENQLSSTRSQLDELEVELKTLRETYNKQHDEWIKEKLSMQEKIKEIEKRNGNGSELDKVRYKAMLDDKQSELDQLRKENEAMHDQMEYMRKETDELRKKLDDYEKVNKVQRNISADSSAMEKEIRQLKAKLSSIEKSKKLELGEYKMRYDNQLSIVNGELQQLQGQVMRFKRERDTYKHMLESAQKTIGDLKNSPRSAKDNTNPSAHYDELEESKTKIATLEQQISCMEDELSEARLECSRLKTELVSERSTWEVKMSELHSRVNELEEEKILSSGRTKIVGLRTRMELAWQKEREEQQRLLQETATLARDLRQTLFEVERERDKERLEAKRKQEQFKKSSEEDQDENKKKITELQCDLLELRDAHAKLRTTNEKLRREKERYEKEREEFKSVIQGKKRLNQEDERKVNVLIEQIDYLKQLAPELFFSRDKEASYTPTPPRRSRSSKSRETSPAIERRESSLSPEDKQQEIQNMMLKLANTAEDLRRLQKLTEDEYERERIKRSMGMRRATSTEHENVSESRYKSYQKRKTDGSLHRKSLSLEHTIAQNDPKIWKNDNDSMSSLHSLDMSSDVERWTTRDTSLDSRLSGGSTQSEFAGEKKKKKGIMGKLKKLTKSRSIDDEDPGTFSPSRSLSSKQNSSSELLEEKGSKKDLRERITGIFKKSGSSSRSNSVERHEVRHDTSSTHRPLMRNGSGGNLSSRASPAFQEPEKTPNAKAKRN

Target ORF information:

RefSeq Version	XM_015978753.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015978753.1

ORF Insert Sequence:

ATGCATCACA TGTACCCCCT TGCCAAGGGA GACCCCTTGT GTCCCCTAGG GTTCCACCCC 
CAAGTCAGGT GGCCCACTAG GTGTAAACGG TGCTTCAGAG ACTATAAGGA ACATGGGGGG 
AAGCGAAGGG ACGAAGAACC GTCCCTCCGA AGAGACGACA CCACGGCGTC GACACCTAGT 
CTGTCTTGGA GTTCAAGGGA TGCAGATAGT GAGAGAAGAC GTAGCTGGGT ATCTAGCAGC 
AACTTAGCGT CTGACCGTGA CAATAATTCT GTAAAAAGTG AAAGTAATTA TAGCAATCCA 
TCATCTTGGA CGTCCACTCC AGATTTAGCA AAATTGGAAG ACGATACTCA AGCTGTCACA 
ACAGTTAGCA TAAAATTACC AAAGAGAAAA CAAAAACCGA TCGATACGGG ACAAAGAACT 
GTTTCTGATA GTTTCTCCAT TAAACCCGAA AAGTCAGTAT TTAGTAAAAA CGATAGTTTA 
GCAGCACGCG CTAGGAAATT ACAGCAAATC AAGGAAGCAG CGGAGGAAAA ACACAAACGA 
ATCCAAATAA AAGAAGTCAA AACAATATCA GAGGAACCAA CAAATCACGA TGTACAATTT 
CTCATCCAAG TGAAACGAAA ACCTACTGTA GTGGAAGACC ATCATTCAGA GCCCGAAGAC 
GACGCTGTAA GTATAGCCGG AACCGAGACA ACTGACACCA CTTTGGTGGA TGCGCACGAT 
CACGAGTTAA GAGAACAGTT AGAGAGTTTA AAAAAAGAGC TAGAAACGAC GAAAACAAAA 
TGTGAAAGAC TTGAACGCGA AAAGAGTGAC ATTCTCCTGC GAAGACTCGC AGCGATGGAA 
ACAACAACCA GCAAGACCAC TGCTTCGGAA GTCCTGAAAC TCCAGCAAAA GTGTAACGAG 
TTGCAACAAA CTCTCGAGGA TTTCCGGGAC GAGAAGAAGA GTCTAACTTT TAAGGTGAAG 
GAGCTGGAGG AGGAGTTGGA ACAGAGACCT ACGGCCCAAG CGGCGCAGAA AATCGCCGAT 
GAGCTGCGTT CGAAGCTGCT CGCGGCTGAA ACACTGTGCG AGGAGCTGAT GGACGAGAAC 
GAAGACATCA AGAAGGAGCT GAGGGACATG GAGGAGCAAA TGGACGAAAT GCAGGACAAC 
TTCCGCGAAG ACCAGGCCGT GGAATACACC TCACTCAAAA AAGAGTTGGA TCAGACCACC 
AAAAACTGCA GGATTTTGTC GTTCAAGCTG CGAAAAGCCG AACGCAAAAC TGAACAGTTG 
GAGCAGGAAA AGAACGAAGC GGAGCGGAAG TTGAAAGAGA TCTCAGGGGG ACAGTCAACC 
TTGGCCAATT TGGAGAAAAT GAAACAACTC GAACAGGATT TGAAGCTAGC TAACGAAGTC 
TCGATTCGGT TGCAAAAGGA GTTGGATGAG ACGAATCAAA AGCTTCAAGA GGAGTCCAAG 
ACGAGCAAAA AGAAAGCGCC GAAGTTGGGA TCCATCGGCA AATTCCCATC TGCTGATGGA 
AAAGTTTCCC GCGAGTCTCT GACTCGGGGC GGTTCCCAAG ACGATCCCGC TCAGTTGCTG 
AGAGACCTCC AAGATTCAAT GGAACGCGAA GCTGACTTAC GGGAACAACT GAAATTTGCT 
GAAGAAGAGA TTTGTAAAAA ATCATGCGGA CATTTCAAAT CCCCCGAATT GCATATTTTT 
CAACGCAAAT CCCCTTTAGT ATTGTTTGAG TCTCAAAACG TGGAGACCAA ACTTTTCACA 
GTATCGACCC AAACTTTGTT AAATCTTGAC TCATCTGAAG CAGGAACACA AGCCGCATTT 
GATATCTGTG ACGCCTCTAG TCAAACAATT GAATGGCGAC CACAAAGTTT CATTTCTTCC 
ATTCAATTCC ATCATCTCAC AATGTCCAAA ATTTACCAAC CAGCGAGACA TTTCTCACCA 
ATGGCCACTT TCCTGAACGC AATGGGTCGT AAACTAAGTC CCACCAATAA TTCAAGATTG 
ACCCCAGAAC CACCTTCTGA GAAAGGTGAC GTATCAGACG ACGAAGACCC CGCAGAAATC 
AAATTACAGT TAGAGTTGAG CGAACAAGAA GCTTCTGTTC TTCGACGCAA AGTTGAAGAT 
TTAGAAGCGG AAAACCATAG AATTAAAACC AAAAACAAGG AACTGCAAGA CAAACTTACC 
GCGAAAACTA CAACAAAACG CACGGCTGTT GGTGGAGAAA AGGGCACGAC ACTGCAAAAT 
CAGAAGCTGA AAGTTCTAGA AGACGAGGCG AACGACCTTC GGAAAAAACT CATCGAGAAG 
GAAAGAGACT GTGAAAGACT ACACGCGGAA CTTAGTCTAA ACCAAAAGAG AAGTAAAAGT 
GTTCAAAAAA GCAAGTCTTT GGATTTGGAT CAGCAAACTT TGGACTTGAA ACGTCAACTT 
CAAGTCATTG AACAAGAAGC GAGCATTTTA CGAAATAAAA TCCAATCGCT TGAGGCAGAG 
AACGAGAAGT TAATAAGCGA GAATAAAAAG CTTCAACTAG TCCGAGGAGC GAAAAACCTC 
AAATCTGCGG ACAAAAACTT AGACAAATAC ATTGACCAAA TAGCTTCGTT AGAAATTGAA 
ATTTCCGAGA AAAACGACAA GATCAAAGCG CTGGAAGAAA AATTAGAAAC GGCCCTTACG 
CAAGATTCAT CAAGTCTTAA AGGAGGAAAT TACAAACGAT TTGTAGAGAG AACACCTAAG 
AAGGTTTCAC AGTTAACATC GAAGGATCAG TTGAAAACTA TGGTGCATGA TTTGGAGAAT 
GAAATTGGTG AGATGATTGT GGCAATAAAA ACCAGCGAAA ACGAGAAGAT TAAACTAGAG 
GAAGAAATGA AGAAAATGCG ACACCAGAAC GAAGTCAATA AAGCGATGCA AGAACTCGAA 
GAAATGAACA AGAAATTTGA AGAGATGAAG ACTGAATTAT CGAAGGAAAA GGAGAAAGTG 
ACTGAAGAGA AAAGTAAATA CGACGAACTT AACAAATCTT TGGTCAAAAC GAAGGAATCC 
TTAACGAAAT CGAATCAAGA GAAGAAGAAA CTTAAAGAAC AAATCGAGAA ATCCAAGGAA 
GAACAGAAGA AAGTACAAGA AGAAAAGGAT AAACTGGACG AAGAAATAGC TAAATTGAAA 
GCTAATTTAA AAACTGCGAC TTATAAACAA GATGAATTGA CCTTAATAAG CCAGAAGGCT 
GAGTCGCTAA AATTAGACCT TGACTCTAAA GAAAAAGAGT TAAAAACCAT CAAAAAAGAA 
CTTGATAGTA AAATCAATGA GTTATCTGAA AAAGCTTCGA AGGTGTCTCA GTTAGAACGG 
AAATTTAGCG AAACTGAAGA AAAATTAAAA ATTGCAGAAA AACGTGAAAA GGATTTGGAA 
GCTAAAATTG AGGAAGAGAA GAGTAAAACC AAGTCGAAAG AAGGAGAGCA AAGTAAATGG 
AACGAAGAGC GCAAAAAGTA CAATAACCAA ATTGAGGAAT TAAATAACAA AATTTTAAGT 
CTGGAAACCA CAGTTGAGAG CAAAAAGAAA TTAATCGAGC GATTGGAAGA GAATTTGAAA 
AAAGAGCGAG AATCGTTTTC CAAAGTTGAT GAACTGGAAA CTCGCGAAAT CACAAAATTA 
AAAGACGAAT TAAGTAAGAG CAAAGCTAAT TTGGCCGACG TTGAGTCCAA ACTGGCATCG 
TCCCAAAAGA GCCAAAAGAA TCTCGAAGAT AAGTTGAAAA AATCGGAGAC GGACAGTAAG 
AATGATAAAC TGTCTCTTGA GAAGAAGAAA GGAGAGTTAG AAATTGAATT ACAAAACGAA 
AAGAAGAAAA TCGAGGTGAT GAAAGGCAAC CACGAGAAAG AGAACAAAAA CAAAGAAATG 
GAGCTCGCTT CGCTGAAAAG CAAGATAAAA TCACTGGAGT TAAACGCCGG AGCTGGGACG 
AAAAGACTAG CAGAAATCAA ACAATTCCAA GAGACTATAG ACAAATTAGA AACTAATCTA 
AATAAAGAAA AGCAGAAATA TGAAGACCTC ACGGCTAAGT ATGAAATTTT GGAAGAAGAA 
CATGTAGTAA CCAAGGCAAA GTTGGTTATG GAGAAAGAAA CTATCGAAAA TCAACTTTCT 
AGTACAAGGT CACAACTGGA CGAATTGGAA GTTGAGTTAA AAACACTGAG AGAAACGTAC 
AACAAACAAC ACGATGAATG GATAAAGGAG AAACTGAGCA TGCAAGAAAA AATTAAAGAG 
ATTGAGAAAC GTAACGGAAA CGGAAGCGAA TTAGATAAAG TGCGATATAA AGCAATGCTG 
GACGATAAGC AATCCGAGTT GGACCAGCTG AGGAAGGAAA ATGAGGCGAT GCACGACCAA 
ATGGAATACA TGAGGAAGGA GACTGACGAA TTGAGGAAAA AACTGGACGA TTACGAAAAA 
GTGAACAAAG TACAAAGGAA CATAAGTGCG GATAGTTCGG CAATGGAGAA GGAAATAAGG 
CAACTTAAAG CAAAGTTGAG TTCTATTGAA AAATCCAAAA AGCTCGAATT GGGCGAGTAC 
AAAATGCGAT ACGACAACCA GCTTTCTATA GTCAACGGCG AACTGCAACA GCTCCAAGGC 
CAAGTGATGC GGTTCAAGCG CGAACGAGAC ACGTACAAGC ACATGCTAGA GTCGGCTCAG 
AAAACTATCG GCGATTTGAA AAATTCTCCA AGAAGTGCCA AAGACAATAC GAATCCTTCT 
GCACACTACG ATGAGTTGGA GGAATCGAAA ACAAAAATTG CAACTCTTGA ACAACAAATC 
AGTTGCATGG AGGACGAACT TTCGGAAGCT CGCCTTGAAT GTTCTCGACT GAAAACCGAA 
CTGGTGTCGG AAAGGTCCAC ATGGGAAGTG AAAATGTCTG AACTGCATTC CCGGGTTAAT 
GAACTAGAAG AGGAGAAAAT CCTTAGTAGC GGAAGAACGA AAATTGTAGG ACTGCGGACT 
CGAATGGAAC TAGCTTGGCA GAAAGAACGC GAGGAACAGC AGAGATTATT GCAGGAAACT 
GCGACTTTGG CCCGAGATCT TCGGCAGACG TTGTTCGAAG TTGAGAGGGA ACGAGACAAG 
GAACGATTAG AAGCGAAACG CAAACAAGAA CAGTTCAAAA AGTCCTCCGA GGAGGATCAA 
GACGAAAATA AGAAGAAAAT AACCGAGCTA CAGTGCGATT TGCTGGAACT AAGAGACGCT 
CACGCCAAAT TGCGAACCAC AAACGAGAAG CTTCGCCGCG AAAAAGAACG CTACGAGAAA 
GAAAGAGAAG AATTCAAGTC GGTTATTCAA GGCAAGAAGC GCCTGAACCA AGAAGACGAA 
CGAAAAGTCA ACGTCCTGAT TGAACAAATC GACTATTTGA AGCAACTAGC ACCAGAACTG 
TTCTTCTCCC GCGATAAGGA AGCCTCATAC ACTCCAACTC CCCCACGGAG GAGCAGAAGC 
TCGAAATCGC GCGAGACTTC ACCAGCCATC GAGCGCCGCG AGTCGTCTTT ATCACCAGAA 
GACAAGCAAC AAGAAATCCA AAACATGATG CTTAAACTGG CAAACACTGC GGAAGACCTG 
CGCCGACTCC AGAAACTAAC CGAAGATGAA TACGAACGGG AGAGAATCAA GCGCAGTATG 
GGGATGAGAA GAGCGACGTC CACTGAACAC GAAAATGTCT CCGAAAGCAG ATACAAGTCG 
TACCAGAAAC GCAAAACCGA CGGGAGCTTA CACCGAAAAA GCTTGTCTCT AGAGCACACC 
ATAGCCCAGA ACGACCCCAA AATCTGGAAA AACGACAACG ACAGCATGTC CAGTCTCCAC 
TCTCTGGACA TGTCTTCAGA CGTCGAAAGG TGGACAACGA GGGACACGAG TCTAGACAGT 
CGCCTCTCTG GTGGTTCCAC CCAGAGCGAG TTCGCCGGCG AGAAGAAAAA GAAGAAGGGA 
ATCATGGGCA AGTTGAAGAA ATTGACCAAA TCTCGAAGCA TCGATGACGA AGATCCTGGG 
ACTTTCTCGC CCTCAAGATC ACTATCGTCA AAGCAAAATT CGAGTTCCGA ACTTCTGGAG 
GAGAAAGGGA GCAAGAAGGA TTTGAGGGAG CGAATCACGG GTATTTTCAA GAAAAGCGGC 
TCGTCATCAC GGAGCAACAG TGTGGAAAGA CACGAAGTGA GACACGACAC AAGTTCGACA 
CACAGGCCCT TGATGCGGAA TGGCAGTGGC GGGAATTTGT CCTCACGAGC GTCTCCAGCT 
TTTCAGGAAC CAGAAAAGAC ACCGAACGCA AAAGCTAAGA GAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC657022 ( XM_015978756.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015834242.1 Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X2, mRNA.

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CloneID	OTg16361
Clone ID Related Accession (Same CDS sequence)	XM_015978756.1
Accession Version	XM_015978756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	centromere-associated protein E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA TGTACCCCCT TGCCAAGGGA GACCCCTTGT GTCCCCTAGG GTTCCACCCC 
CAAGTCAGGT GGCCCACTAG GTGTAAACGG TGCTTCAGAG ACTATAAGGA ACATGGGGGG 
AAGCGAAGGG ACGAAGAACC GTCCCTCCGA AGAGACGACA CCACGGCGTC GACACCTAGT 
CTGTCTTGGA GTTCAAGGGA TGCAGATAGT GAGAGAAGAC GTAGCTGGGT ATCTAGCAGC 
AACTTAGCGT CTGACCGTGA CAATAATTCT GTAAAAAGTG AAAGTAATTA TAGCAATCCA 
TCATCTTGGA CGTCCACTCC AGATTTAGCA AAATTGGAAG ACGATACTCA AGCTGTCACA 
ACAGTTAGCA TAAAATTACC AAAGAGAAAA CAAAAACCGA TCGATACGGG ACAAAGAACT 
GTTTCTGATA GTTTCTCCAT TAAACCCGAA AAGTCAGTAT TTAGTAAAAA CGATAGTTTA 
GCAGCACGCG CTAGGAAATT ACAGCAAATC AAGGAAGCAG CGGAGGAAAA ACACAAACGA 
ATCCAAATAA AAGAAGTCAA AACAATATCA GAGGAACCAA CAAATCACGA TGTACAATTT 
CTCATCCAAG TGAAACGAAA ACCTACTGTA GTGGAAGACC ATCATTCAGA GCCCGAAGAC 
GACGCTGTAA GTATAGCCGG AACCGAGACA ACTGACACCA CTTTGGTGGA TGCGCACGAT 
CACGAGTTAA GAGAACAGTT AGAGAGTTTA AAAAAAGAGC TAGAAACGAC GAAAACAAAA 
TGTGAAAGAC TTGAACGCGA AAAGAGTGAC ATTCTCCTGC GAAGACTCGC AGCGATGGAA 
ACAACAACCA GCAAGACCAC TGCTTCGGAA GTCCTGAAAC TCCAGCAAAA GTGTAACGAG 
TTGCAACAAA CTCTCGAGGA TTTCCGGGAC GAGAAGAAGA GTCTAACTTT TAAGGTGAAG 
GAGCTGGAGG AGGAGTTGGA ACAGAGACCT ACGGCCCAAG CGGCGCAGAA AATCGCCGAT 
GAGCTGCGTT CGAAGCTGCT CGCGGCTGAA ACACTGTGCG AGGAGCTGAT GGACGAGAAC 
GAAGACATCA AGAAGGAGCT GAGGGACATG GAGGAGCAAA TGGACGAAAT GCAGGACAAC 
TTCCGCGAAG ACCAGGCCGT GGAATACACC TCACTCAAAA AAGAGTTGGA TCAGACCACC 
AAAAACTGCA GGATTTTGTC GTTCAAGCTG CGAAAAGCCG AACGCAAAAC TGAACAGTTG 
GAGCAGGAAA AGAACGAAGC GGAGCGGAAG TTGAAAGAGA TCTCAGGGGG ACAGTCAACC 
TTGGCCAATT TGGAGAAAAT GAAACAACTC GAACAGGATT TGAAGCTAGC TAACGAAGTC 
TCGATTCGGT TGCAAAAGGA GTTGGATGAG ACGAATCAAA AGCTTCAAGA GGAGTCCAAG 
ACGAGCAAAA AGAAAGCGCC GAAGTTGGGA TCCATCGGCA AATTCCCATC TGCTGATGGA 
AAAGTTTCCC GCGAGTCTCT GACTCGGGGC GGTTCCCAAG ACGATCCCGC TCAGTTGCTG 
AGAGACCTCC AAGATTCAAT GGAACGCGAA GCTGACTTAC GGGAACAACT GAAATTTGCT 
GAAGAAGAGA TTTGTAAAAA ATCATGCGGA CATTTCAAAT CCCCCGAATT GCATATTTTT 
CAACGCAAAT CCCCTTTAGT ATTGTTTGAG TCTCAAAACG TGGAGACCAA ACTTTTCACA 
GTATCGACCC AAACTTTGTT AAATCTTGAC TCATCTGAAG CAGGAACACA AGCCGCATTT 
GATATCTGTG ACGCCTCTAG TCAAACAATT GAATGGCGAC CACAAAGTTT CATTTCTTCC 
ATTCAATTCC ATCATCTCAC AATGTCCAAA ATTTACCAAC CAGCGAGACA TTTCTCACCA 
ATGGCCACTT TCCTGAACGC AATGGGTCGT AAACTAAGTC CCACCAATAA TTCAAGATTG 
ACCCCAGAAC CACCTTCTGA GAAAGGTGAC GTATCAGACG ACGAAGACCC CGCAGAAATC 
AAATTACAGT TAGAGTTGAG CGAACAAGAA GCTTCTGTTC TTCGACGCAA AGTTGAAGAT 
TTAGAAGCGG AAAACCATAG AATTAAAACC AAAAACAAGG AACTGCAAGA CAAACTTACC 
GCGAAAACTA CAACAAAACG CACGGCTGTT GGTGGAGAAA AGGGCACGAC ACTGCAAAAT 
CAGAAGCTGA AAGTTCTAGA AGACGAGGCG AACGACCTTC GGAAAAAACT CATCGAGAAG 
GAAAGAGACT GTGAAAGACT ACACGCGGAA CTTAGTCTAA ACCAAAAGAG AAGTAAAAGT 
GTTCAAAAAA GCAAGTCTTT GGATTTGGAT CAGCAAACTT TGGACTTGAA ACGTCAACTT 
CAAGTCATTG AACAAGAAGC GAGCATTTTA CGAAATAAAA TCCAATCGCT TGAGGCAGAG 
AACGAGAAGT TAATAAGCGA GAATAAAAAG CTTCAACTAG TCCGAGGAGC GAAAAACCTC 
AAATCTGCGG ACAAAAACTT AGACAAATAC ATTGACCAAA TAGCTTCGTT AGAAATTGAA 
ATTTCCGAGA AAAACGACAA GATCAAAGCG CTGGAAGAAA AATTAGAAAC GGCCCTTACG 
CAAGATTCAT CAAGTCTTAA AGGAGGAAAT TACAAACGAT TTGTAGAGAG AACACCTAAG 
AAGGTTTCAC AGTTAACATC GAAGGATCAG TTGAAAACTA TGGTGCATGA TTTGGAGAAT 
GAAATTGGTG AGATGATTGT GGCAATAAAA ACCAGCGAAA ACGAGAAGAT TAAACTAGAG 
GAAGAAATGA AGAAAATGCG ACACCAGAAC GAAGTCAATA AAGCGATGCA AGAACTCGAA 
GAAATGAACA AGAAATTTGA AGAGATGAAG ACTGAATTAT CGAAGGAAAA GGAGAAAGTG 
ACTGAAGAGA AAAGTAAATA CGACGAACTT AACAAATCTT TGGTCAAAAC GAAGGAATCC 
TTAACGAAAT CGAATCAAGA GAAGAAGAAA CTTAAAGAAC AAATCGAGAA ATCCAAGGAA 
GAACAGAAGA AAGTACAAGA AGAAAAGGAT AAACTGGACG AAGAAATAGC TAAATTGAAA 
GCTAATTTAA AAACTGCGAC TTATAAACAA GATGAATTGA CCTTAATAAG CCAGAAGGCT 
GAGTCGCTAA AATTAGACCT TGACTCTAAA GAAAAAGAGT TAAAAACCAT CAAAAAAGAA 
CTTGATAGTA AAATCAATGA GTTATCTGAA AAAGCTTCGA AGGTGTCTCA GTTAGAACGG 
AAATTTAGCG AAACTGAAGA AAAATTAAAA ATTGCAGAAA AACGTGAAAA GGATTTGGAA 
GCTAAAATTG AGGAAGAGAA GAGTAAAACC AAGTCGAAAG AAGGAGAGCA AAGTAAATGG 
AACGAAGAGC GCAAAAAGTA CAATAACCAA ATTGAGGAAT TAAATAACAA AATTTTAAGT 
CTGGAAACCA CAGTTGAGAG CAAAAAGAAA TTAATCGAGC GATTGGAAGA GAATTTGAAA 
AAAGAGCGAG AATCGTTTTC CAAAGTTGAT GAACTGGAAA CTCGCGAAAT CACAAAATTA 
AAAGACGAAT TAAGTAAGAG CAAAGCTAAT TTGGCCGACG TTGAGTCCAA ACTGGCATCG 
TCCCAAAAGA GCCAAAAGAA TCTCGAAGAT AAGTTGAAAA AATCGGAGAC GGACAGTAAG 
AATGATAAAC TGTCTCTTGA GAAGAAGAAA GGAGAGTTAG AAATTGAATT ACAAAACGAA 
AAGAAGAAAA TCGAGGTGAT GAAAGGCAAC CACGAGAAAG AGAACAAAAA CAAAGAAATG 
GAGCTCGCTT CGCTGAAAAG CAAGATAAAA TCACTGGAGT TAAACGCCGG AGCTGGGACG 
AAAAGACTAG CAGAAATCAA ACAATTCCAA GAGACTATAG ACAAATTAGA AACTAATCTA 
AATAAAGAAA AGCAGAAATA TGAAGACCTC ACGGCTAAGT ATGAAATTTT GGAAGAAGAA 
CATGTAGTAA CCAAGGCAAA GTTGGTTATG GAGAAAGAAA CTATCGAAAA TCAACTTTCT 
AGTACAAGGT CACAACTGGA CGAATTGGAA GTTGAGTTAA AAACACTGAG AGAAACGTAC 
AACAAACAAC ACGATGAATG GATAAAGGAG AAACTGAGCA TGCAAGAAAA AATTAAAGAG 
ATTGAGAAAC GTAACGGAAA CGGAAGCGAA TTAGATAAAG TGCGATATAA AGCAATGCTG 
GACGATAAGC AATCCGAGTT GGACCAGCTG AGGAAGGAAA ATGAGGCGAT GCACGACCAA 
ATGGAATACA TGAGGAAGGA GACTGACGAA TTGAGGAAAA AACTGGACGA TTACGAAAAA 
GTGAACAAAG TACAAAGGAA CATAAGTGCG GATAGTTCGG CAATGGAGAA GGAAATAAGG 
CAACTTAAAG CAAAGTTGAG TTCTATTGAA AAATCCAAAA AGCTCGAATT GGGCGAGTAC 
AAAATGCGAT ACGACAACCA GCTTTCTATA GTCAACGGCG AACTGCAACA GCTCCAAGGC 
CAAGTGATGC GGTTCAAGCG CGAACGAGAC ACGTACAAGC ACATGCTAGA GTCGGCTCAG 
AAAACTATCG GCGATTTGAA AAATTCTCCA AGAAGTGCCA AAGACAATAC GAATCCTTCT 
GCACACTACG ATGAGTTGGA GGAATCGAAA ACAAAAATTG CAACTCTTGA ACAACAAATC 
AGTTGCATGG AGGACGAACT TTCGGAAGCT CGCCTTGAAT GTTCTCGACT GAAAACCGAA 
CTGGTGTCGG AAAGGTCCAC ATGGGAAGTG AAAATGTCTG AACTGCATTC CCGGGTTAAT 
GAACTAGAAG AGGAGAAAAT CCTTAGTAGC GGAAGAACGA AAATTGTAGG ACTGCGGACT 
CGAATGGAAC TAGCTTGGCA GAAAGAACGC GAGGAACAGC AGAGATTATT GCAGGAAACT 
GCGACTTTGG CCCGAGATCT TCGGCAGACG TTGTTCGAAG TTGAGAGGGA ACGAGACAAG 
GAACGATTAG AAGCGAAACG CAAACAAGAA CAGTTCAAAA AGTCCTCCGA GGAGGATCAA 
GACGAAAATA AGAAGAAAAT AACCGAGCTA CAGTGCGATT TGCTGGAACT AAGAGACGCT 
CACGCCAAAT TGCGAACCAC AAACGAGAAG CTTCGCCGCG AAAAAGAACG CTACGAGAAA 
GAAAGAGAAG AATTCAAGTC GGTTATTCAA GGCAAGAAGC GCCTGAACCA AGAAGACGAA 
CGAAAAGTCA ACGTCCTGAT TGAACAAATC GACTATTTGA AGCAACTAGC ACCAGAACTG 
TTCTTCTCCC GCGATAAGGA AGCCTCATAC ACTCCAACTC CCCCACGGAG GAGCAGAAGC 
TCGAAATCGC GCGAGACTTC ACCAGCCATC GAGCGCCGCG AGTCGTCTTT ATCACCAGAA 
GACAAGCAAC AAGAAATCCA AAACATGATG CTTAAACTGG CAAACACTGC GGAAGACCTG 
CGCCGACTCC AGAAACTAAC CGAAGATGAA TACGAACGGG AGAGAATCAA GCGCAGTATG 
GGGATGAGAA GAGCGACGTC CACTGAACAC GAAAATGTCT CCGAAAGCAG ATACAAGTCG 
TACCAGAAAC GCAAAACCGA CGGGAGCTTA CACCGAAAAA GCTTGTCTCT AGAGCACACC 
ATAGCCCAGA ACGACCCCAA AATCTGGAAA AACGACAACG ACAGCATGTC CAGTCTCCAC 
TCTCTGGACA TGTCTTCAGA CGTCGAAAGG TGGACAACGA GGGACACGAG TCTAGACAGT 
CGCCTCTCTG GTGGTTCCAC CCAGAGCGAG TTCGCCGGCG AGAAGAAAAA GAAGAAGGGA 
ATCATGGGCA AGTTGAAGAA ATTGACCAAA TCTCGAAGCA TCGATGACGA AGATCCTGGG 
ACTTTCTCGC CCTCAAGATC ACTATCGTCA AAGCAAAATT CGAGTTCCGA ACTTCTGGAG 
GAGAAAGGGA GCAAGAAGGA TTTGAGGGAG CGAATCACGG GTATTTTCAA GAAAAGCGGC 
TCGTCATCAC GGAGCAACAG TGTGGAAAGA CACGAAGTGA GACACGACAC AAGTTCGACA 
CACAGGCCCT TGATGCGGAA TGGCAGTGGC GGGAATTTGT CCTCACGAGC GTCTCCAGCT 
GAACCAGAAA AGACACCGAA CGCAAAAGCT AAGAGAAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015834242.1
CDS	475..6696
Translation	MHHMYPLAKGDPLCPLGFHPQVRWPTRCKRCFRDYKEHGGKRRDEEPSLRRDDTTASTPSLSWSSRDADSERRRSWVSSSNLASDRDNNSVKSESNYSNPSSWTSTPDLAKLEDDTQAVTTVSIKLPKRKQKPIDTGQRTVSDSFSIKPEKSVFSKNDSLAARARKLQQIKEAAEEKHKRIQIKEVKTISEEPTNHDVQFLIQVKRKPTVVEDHHSEPEDDAVSIAGTETTDTTLVDAHDHELREQLESLKKELETTKTKCERLEREKSDILLRRLAAMETTTSKTTASEVLKLQQKCNELQQTLEDFRDEKKSLTFKVKELEEELEQRPTAQAAQKIADELRSKLLAAETLCEELMDENEDIKKELRDMEEQMDEMQDNFREDQAVEYTSLKKELDQTTKNCRILSFKLRKAERKTEQLEQEKNEAERKLKEISGGQSTLANLEKMKQLEQDLKLANEVSIRLQKELDETNQKLQEESKTSKKKAPKLGSIGKFPSADGKVSRESLTRGGSQDDPAQLLRDLQDSMEREADLREQLKFAEEEICKKSCGHFKSPELHIFQRKSPLVLFESQNVETKLFTVSTQTLLNLDSSEAGTQAAFDICDASSQTIEWRPQSFISSIQFHHLTMSKIYQPARHFSPMATFLNAMGRKLSPTNNSRLTPEPPSEKGDVSDDEDPAEIKLQLELSEQEASVLRRKVEDLEAENHRIKTKNKELQDKLTAKTTTKRTAVGGEKGTTLQNQKLKVLEDEANDLRKKLIEKERDCERLHAELSLNQKRSKSVQKSKSLDLDQQTLDLKRQLQVIEQEASILRNKIQSLEAENEKLISENKKLQLVRGAKNLKSADKNLDKYIDQIASLEIEISEKNDKIKALEEKLETALTQDSSSLKGGNYKRFVERTPKKVSQLTSKDQLKTMVHDLENEIGEMIVAIKTSENEKIKLEEEMKKMRHQNEVNKAMQELEEMNKKFEEMKTELSKEKEKVTEEKSKYDELNKSLVKTKESLTKSNQEKKKLKEQIEKSKEEQKKVQEEKDKLDEEIAKLKANLKTATYKQDELTLISQKAESLKLDLDSKEKELKTIKKELDSKINELSEKASKVSQLERKFSETEEKLKIAEKREKDLEAKIEEEKSKTKSKEGEQSKWNEERKKYNNQIEELNNKILSLETTVESKKKLIERLEENLKKERESFSKVDELETREITKLKDELSKSKANLADVESKLASSQKSQKNLEDKLKKSETDSKNDKLSLEKKKGELEIELQNEKKKIEVMKGNHEKENKNKEMELASLKSKIKSLELNAGAGTKRLAEIKQFQETIDKLETNLNKEKQKYEDLTAKYEILEEEHVVTKAKLVMEKETIENQLSSTRSQLDELEVELKTLRETYNKQHDEWIKEKLSMQEKIKEIEKRNGNGSELDKVRYKAMLDDKQSELDQLRKENEAMHDQMEYMRKETDELRKKLDDYEKVNKVQRNISADSSAMEKEIRQLKAKLSSIEKSKKLELGEYKMRYDNQLSIVNGELQQLQGQVMRFKRERDTYKHMLESAQKTIGDLKNSPRSAKDNTNPSAHYDELEESKTKIATLEQQISCMEDELSEARLECSRLKTELVSERSTWEVKMSELHSRVNELEEEKILSSGRTKIVGLRTRMELAWQKEREEQQRLLQETATLARDLRQTLFEVERERDKERLEAKRKQEQFKKSSEEDQDENKKKITELQCDLLELRDAHAKLRTTNEKLRREKERYEKEREEFKSVIQGKKRLNQEDERKVNVLIEQIDYLKQLAPELFFSRDKEASYTPTPPRRSRSSKSRETSPAIERRESSLSPEDKQQEIQNMMLKLANTAEDLRRLQKLTEDEYERERIKRSMGMRRATSTEHENVSESRYKSYQKRKTDGSLHRKSLSLEHTIAQNDPKIWKNDNDSMSSLHSLDMSSDVERWTTRDTSLDSRLSGGSTQSEFAGEKKKKKGIMGKLKKLTKSRSIDDEDPGTFSPSRSLSSKQNSSSELLEEKGSKKDLRERITGIFKKSGSSSRSNSVERHEVRHDTSSTHRPLMRNGSGGNLSSRASPAEPEKTPNAKAKRN

Target ORF information:

RefSeq Version	XM_015978756.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015978756.1

ORF Insert Sequence:

ATGCATCACA TGTACCCCCT TGCCAAGGGA GACCCCTTGT GTCCCCTAGG GTTCCACCCC 
CAAGTCAGGT GGCCCACTAG GTGTAAACGG TGCTTCAGAG ACTATAAGGA ACATGGGGGG 
AAGCGAAGGG ACGAAGAACC GTCCCTCCGA AGAGACGACA CCACGGCGTC GACACCTAGT 
CTGTCTTGGA GTTCAAGGGA TGCAGATAGT GAGAGAAGAC GTAGCTGGGT ATCTAGCAGC 
AACTTAGCGT CTGACCGTGA CAATAATTCT GTAAAAAGTG AAAGTAATTA TAGCAATCCA 
TCATCTTGGA CGTCCACTCC AGATTTAGCA AAATTGGAAG ACGATACTCA AGCTGTCACA 
ACAGTTAGCA TAAAATTACC AAAGAGAAAA CAAAAACCGA TCGATACGGG ACAAAGAACT 
GTTTCTGATA GTTTCTCCAT TAAACCCGAA AAGTCAGTAT TTAGTAAAAA CGATAGTTTA 
GCAGCACGCG CTAGGAAATT ACAGCAAATC AAGGAAGCAG CGGAGGAAAA ACACAAACGA 
ATCCAAATAA AAGAAGTCAA AACAATATCA GAGGAACCAA CAAATCACGA TGTACAATTT 
CTCATCCAAG TGAAACGAAA ACCTACTGTA GTGGAAGACC ATCATTCAGA GCCCGAAGAC 
GACGCTGTAA GTATAGCCGG AACCGAGACA ACTGACACCA CTTTGGTGGA TGCGCACGAT 
CACGAGTTAA GAGAACAGTT AGAGAGTTTA AAAAAAGAGC TAGAAACGAC GAAAACAAAA 
TGTGAAAGAC TTGAACGCGA AAAGAGTGAC ATTCTCCTGC GAAGACTCGC AGCGATGGAA 
ACAACAACCA GCAAGACCAC TGCTTCGGAA GTCCTGAAAC TCCAGCAAAA GTGTAACGAG 
TTGCAACAAA CTCTCGAGGA TTTCCGGGAC GAGAAGAAGA GTCTAACTTT TAAGGTGAAG 
GAGCTGGAGG AGGAGTTGGA ACAGAGACCT ACGGCCCAAG CGGCGCAGAA AATCGCCGAT 
GAGCTGCGTT CGAAGCTGCT CGCGGCTGAA ACACTGTGCG AGGAGCTGAT GGACGAGAAC 
GAAGACATCA AGAAGGAGCT GAGGGACATG GAGGAGCAAA TGGACGAAAT GCAGGACAAC 
TTCCGCGAAG ACCAGGCCGT GGAATACACC TCACTCAAAA AAGAGTTGGA TCAGACCACC 
AAAAACTGCA GGATTTTGTC GTTCAAGCTG CGAAAAGCCG AACGCAAAAC TGAACAGTTG 
GAGCAGGAAA AGAACGAAGC GGAGCGGAAG TTGAAAGAGA TCTCAGGGGG ACAGTCAACC 
TTGGCCAATT TGGAGAAAAT GAAACAACTC GAACAGGATT TGAAGCTAGC TAACGAAGTC 
TCGATTCGGT TGCAAAAGGA GTTGGATGAG ACGAATCAAA AGCTTCAAGA GGAGTCCAAG 
ACGAGCAAAA AGAAAGCGCC GAAGTTGGGA TCCATCGGCA AATTCCCATC TGCTGATGGA 
AAAGTTTCCC GCGAGTCTCT GACTCGGGGC GGTTCCCAAG ACGATCCCGC TCAGTTGCTG 
AGAGACCTCC AAGATTCAAT GGAACGCGAA GCTGACTTAC GGGAACAACT GAAATTTGCT 
GAAGAAGAGA TTTGTAAAAA ATCATGCGGA CATTTCAAAT CCCCCGAATT GCATATTTTT 
CAACGCAAAT CCCCTTTAGT ATTGTTTGAG TCTCAAAACG TGGAGACCAA ACTTTTCACA 
GTATCGACCC AAACTTTGTT AAATCTTGAC TCATCTGAAG CAGGAACACA AGCCGCATTT 
GATATCTGTG ACGCCTCTAG TCAAACAATT GAATGGCGAC CACAAAGTTT CATTTCTTCC 
ATTCAATTCC ATCATCTCAC AATGTCCAAA ATTTACCAAC CAGCGAGACA TTTCTCACCA 
ATGGCCACTT TCCTGAACGC AATGGGTCGT AAACTAAGTC CCACCAATAA TTCAAGATTG 
ACCCCAGAAC CACCTTCTGA GAAAGGTGAC GTATCAGACG ACGAAGACCC CGCAGAAATC 
AAATTACAGT TAGAGTTGAG CGAACAAGAA GCTTCTGTTC TTCGACGCAA AGTTGAAGAT 
TTAGAAGCGG AAAACCATAG AATTAAAACC AAAAACAAGG AACTGCAAGA CAAACTTACC 
GCGAAAACTA CAACAAAACG CACGGCTGTT GGTGGAGAAA AGGGCACGAC ACTGCAAAAT 
CAGAAGCTGA AAGTTCTAGA AGACGAGGCG AACGACCTTC GGAAAAAACT CATCGAGAAG 
GAAAGAGACT GTGAAAGACT ACACGCGGAA CTTAGTCTAA ACCAAAAGAG AAGTAAAAGT 
GTTCAAAAAA GCAAGTCTTT GGATTTGGAT CAGCAAACTT TGGACTTGAA ACGTCAACTT 
CAAGTCATTG AACAAGAAGC GAGCATTTTA CGAAATAAAA TCCAATCGCT TGAGGCAGAG 
AACGAGAAGT TAATAAGCGA GAATAAAAAG CTTCAACTAG TCCGAGGAGC GAAAAACCTC 
AAATCTGCGG ACAAAAACTT AGACAAATAC ATTGACCAAA TAGCTTCGTT AGAAATTGAA 
ATTTCCGAGA AAAACGACAA GATCAAAGCG CTGGAAGAAA AATTAGAAAC GGCCCTTACG 
CAAGATTCAT CAAGTCTTAA AGGAGGAAAT TACAAACGAT TTGTAGAGAG AACACCTAAG 
AAGGTTTCAC AGTTAACATC GAAGGATCAG TTGAAAACTA TGGTGCATGA TTTGGAGAAT 
GAAATTGGTG AGATGATTGT GGCAATAAAA ACCAGCGAAA ACGAGAAGAT TAAACTAGAG 
GAAGAAATGA AGAAAATGCG ACACCAGAAC GAAGTCAATA AAGCGATGCA AGAACTCGAA 
GAAATGAACA AGAAATTTGA AGAGATGAAG ACTGAATTAT CGAAGGAAAA GGAGAAAGTG 
ACTGAAGAGA AAAGTAAATA CGACGAACTT AACAAATCTT TGGTCAAAAC GAAGGAATCC 
TTAACGAAAT CGAATCAAGA GAAGAAGAAA CTTAAAGAAC AAATCGAGAA ATCCAAGGAA 
GAACAGAAGA AAGTACAAGA AGAAAAGGAT AAACTGGACG AAGAAATAGC TAAATTGAAA 
GCTAATTTAA AAACTGCGAC TTATAAACAA GATGAATTGA CCTTAATAAG CCAGAAGGCT 
GAGTCGCTAA AATTAGACCT TGACTCTAAA GAAAAAGAGT TAAAAACCAT CAAAAAAGAA 
CTTGATAGTA AAATCAATGA GTTATCTGAA AAAGCTTCGA AGGTGTCTCA GTTAGAACGG 
AAATTTAGCG AAACTGAAGA AAAATTAAAA ATTGCAGAAA AACGTGAAAA GGATTTGGAA 
GCTAAAATTG AGGAAGAGAA GAGTAAAACC AAGTCGAAAG AAGGAGAGCA AAGTAAATGG 
AACGAAGAGC GCAAAAAGTA CAATAACCAA ATTGAGGAAT TAAATAACAA AATTTTAAGT 
CTGGAAACCA CAGTTGAGAG CAAAAAGAAA TTAATCGAGC GATTGGAAGA GAATTTGAAA 
AAAGAGCGAG AATCGTTTTC CAAAGTTGAT GAACTGGAAA CTCGCGAAAT CACAAAATTA 
AAAGACGAAT TAAGTAAGAG CAAAGCTAAT TTGGCCGACG TTGAGTCCAA ACTGGCATCG 
TCCCAAAAGA GCCAAAAGAA TCTCGAAGAT AAGTTGAAAA AATCGGAGAC GGACAGTAAG 
AATGATAAAC TGTCTCTTGA GAAGAAGAAA GGAGAGTTAG AAATTGAATT ACAAAACGAA 
AAGAAGAAAA TCGAGGTGAT GAAAGGCAAC CACGAGAAAG AGAACAAAAA CAAAGAAATG 
GAGCTCGCTT CGCTGAAAAG CAAGATAAAA TCACTGGAGT TAAACGCCGG AGCTGGGACG 
AAAAGACTAG CAGAAATCAA ACAATTCCAA GAGACTATAG ACAAATTAGA AACTAATCTA 
AATAAAGAAA AGCAGAAATA TGAAGACCTC ACGGCTAAGT ATGAAATTTT GGAAGAAGAA 
CATGTAGTAA CCAAGGCAAA GTTGGTTATG GAGAAAGAAA CTATCGAAAA TCAACTTTCT 
AGTACAAGGT CACAACTGGA CGAATTGGAA GTTGAGTTAA AAACACTGAG AGAAACGTAC 
AACAAACAAC ACGATGAATG GATAAAGGAG AAACTGAGCA TGCAAGAAAA AATTAAAGAG 
ATTGAGAAAC GTAACGGAAA CGGAAGCGAA TTAGATAAAG TGCGATATAA AGCAATGCTG 
GACGATAAGC AATCCGAGTT GGACCAGCTG AGGAAGGAAA ATGAGGCGAT GCACGACCAA 
ATGGAATACA TGAGGAAGGA GACTGACGAA TTGAGGAAAA AACTGGACGA TTACGAAAAA 
GTGAACAAAG TACAAAGGAA CATAAGTGCG GATAGTTCGG CAATGGAGAA GGAAATAAGG 
CAACTTAAAG CAAAGTTGAG TTCTATTGAA AAATCCAAAA AGCTCGAATT GGGCGAGTAC 
AAAATGCGAT ACGACAACCA GCTTTCTATA GTCAACGGCG AACTGCAACA GCTCCAAGGC 
CAAGTGATGC GGTTCAAGCG CGAACGAGAC ACGTACAAGC ACATGCTAGA GTCGGCTCAG 
AAAACTATCG GCGATTTGAA AAATTCTCCA AGAAGTGCCA AAGACAATAC GAATCCTTCT 
GCACACTACG ATGAGTTGGA GGAATCGAAA ACAAAAATTG CAACTCTTGA ACAACAAATC 
AGTTGCATGG AGGACGAACT TTCGGAAGCT CGCCTTGAAT GTTCTCGACT GAAAACCGAA 
CTGGTGTCGG AAAGGTCCAC ATGGGAAGTG AAAATGTCTG AACTGCATTC CCGGGTTAAT 
GAACTAGAAG AGGAGAAAAT CCTTAGTAGC GGAAGAACGA AAATTGTAGG ACTGCGGACT 
CGAATGGAAC TAGCTTGGCA GAAAGAACGC GAGGAACAGC AGAGATTATT GCAGGAAACT 
GCGACTTTGG CCCGAGATCT TCGGCAGACG TTGTTCGAAG TTGAGAGGGA ACGAGACAAG 
GAACGATTAG AAGCGAAACG CAAACAAGAA CAGTTCAAAA AGTCCTCCGA GGAGGATCAA 
GACGAAAATA AGAAGAAAAT AACCGAGCTA CAGTGCGATT TGCTGGAACT AAGAGACGCT 
CACGCCAAAT TGCGAACCAC AAACGAGAAG CTTCGCCGCG AAAAAGAACG CTACGAGAAA 
GAAAGAGAAG AATTCAAGTC GGTTATTCAA GGCAAGAAGC GCCTGAACCA AGAAGACGAA 
CGAAAAGTCA ACGTCCTGAT TGAACAAATC GACTATTTGA AGCAACTAGC ACCAGAACTG 
TTCTTCTCCC GCGATAAGGA AGCCTCATAC ACTCCAACTC CCCCACGGAG GAGCAGAAGC 
TCGAAATCGC GCGAGACTTC ACCAGCCATC GAGCGCCGCG AGTCGTCTTT ATCACCAGAA 
GACAAGCAAC AAGAAATCCA AAACATGATG CTTAAACTGG CAAACACTGC GGAAGACCTG 
CGCCGACTCC AGAAACTAAC CGAAGATGAA TACGAACGGG AGAGAATCAA GCGCAGTATG 
GGGATGAGAA GAGCGACGTC CACTGAACAC GAAAATGTCT CCGAAAGCAG ATACAAGTCG 
TACCAGAAAC GCAAAACCGA CGGGAGCTTA CACCGAAAAA GCTTGTCTCT AGAGCACACC 
ATAGCCCAGA ACGACCCCAA AATCTGGAAA AACGACAACG ACAGCATGTC CAGTCTCCAC 
TCTCTGGACA TGTCTTCAGA CGTCGAAAGG TGGACAACGA GGGACACGAG TCTAGACAGT 
CGCCTCTCTG GTGGTTCCAC CCAGAGCGAG TTCGCCGGCG AGAAGAAAAA GAAGAAGGGA 
ATCATGGGCA AGTTGAAGAA ATTGACCAAA TCTCGAAGCA TCGATGACGA AGATCCTGGG 
ACTTTCTCGC CCTCAAGATC ACTATCGTCA AAGCAAAATT CGAGTTCCGA ACTTCTGGAG 
GAGAAAGGGA GCAAGAAGGA TTTGAGGGAG CGAATCACGG GTATTTTCAA GAAAAGCGGC 
TCGTCATCAC GGAGCAACAG TGTGGAAAGA CACGAAGTGA GACACGACAC AAGTTCGACA 
CACAGGCCCT TGATGCGGAA TGGCAGTGGC GGGAATTTGT CCTCACGAGC GTCTCCAGCT 
GAACCAGAAA AGACACCGAA CGCAAAAGCT AAGAGAAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC657022 ( XM_015978760.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015834246.1 Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X3, mRNA.

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CloneID	OTg16362
Clone ID Related Accession (Same CDS sequence)	XM_015978760.1
Accession Version	XM_015978760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	centromere-associated protein E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCACA TGTACCCCCT TGCCAAGGGA GACCCCTTGT GTCCCCTAGG GTTCCACCCC 
CAAGTCAGGT GGCCCACTAG GTGTAAACGG TGCTTCAGAG ACTATAAGGA ACATGGGGGG 
AAGCGAAGGG ACGAAGAACC GTCCCTCCGA AGAGACGACA CCACGGCGTC GACACCTAGT 
CTGTCTTGGA GTTCAAGGGA TGCAGATAGT GAGAGAAGAC GTAGCTGGGT ATCTAGCAGC 
AACTTAGCGT CTGACCGTGA CAATAATTCT GTAAAAAGTG AAAGTAATTA TAGCAATCCA 
TCATCTTGGA CGTCCACTCC AGATTTAGCA AAATTGGAAG ACGATACTCA AGCTGTCACA 
ACAGTTAGCA TAAAATTACC AAAGAGAAAA CAAAAACCGA TCGATACGGG ACAAAGAACT 
GTTTCTGATA GTTTCTCCAT TAAACCCGAA AAGTCAGTAT TTAGTAAAAA CGATAGTTTA 
GCAGCACGCG CTAGGAAATT ACAGCAAATC AAGGAAGCAG CGGAGGAAAA ACACAAACGA 
ATCCAAATAA AAGAAGTCAA AACAATATCA GAGGAACCAA CAAATCACGA TGTGAAACGA 
AAACCTACTG TAGTGGAAGA CCATCATTCA GAGCCCGAAG ACGACGCTGT AAGTATAGCC 
GGAACCGAGA CAACTGACAC CACTTTGGTG GATGCGCACG ATCACGAGTT AAGAGAACAG 
TTAGAGAGTT TAAAAAAAGA GCTAGAAACG ACGAAAACAA AATGTGAAAG ACTTGAACGC 
GAAAAGAGTG ACATTCTCCT GCGAAGACTC GCAGCGATGG AAACAACAAC CAGCAAGACC 
ACTGCTTCGG AAGTCCTGAA ACTCCAGCAA AAGTGTAACG AGTTGCAACA AACTCTCGAG 
GATTTCCGGG ACGAGAAGAA GAGTCTAACT TTTAAGGTGA AGGAGCTGGA GGAGGAGTTG 
GAACAGAGAC CTACGGCCCA AGCGGCGCAG AAAATCGCCG ATGAGCTGCG TTCGAAGCTG 
CTCGCGGCTG AAACACTGTG CGAGGAGCTG ATGGACGAGA ACGAAGACAT CAAGAAGGAG 
CTGAGGGACA TGGAGGAGCA AATGGACGAA ATGCAGGACA ACTTCCGCGA AGACCAGGCC 
GTGGAATACA CCTCACTCAA AAAAGAGTTG GATCAGACCA CCAAAAACTG CAGGATTTTG 
TCGTTCAAGC TGCGAAAAGC CGAACGCAAA ACTGAACAGT TGGAGCAGGA AAAGAACGAA 
GCGGAGCGGA AGTTGAAAGA GATCTCAGGG GGACAGTCAA CCTTGGCCAA TTTGGAGAAA 
ATGAAACAAC TCGAACAGGA TTTGAAGCTA GCTAACGAAG TCTCGATTCG GTTGCAAAAG 
GAGTTGGATG AGACGAATCA AAAGCTTCAA GAGGAGTCCA AGACGAGCAA AAAGAAAGCG 
CCGAAGTTGG GATCCATCGG CAAATTCCCA TCTGCTGATG GAAAAGTTTC CCGCGAGTCT 
CTGACTCGGG GCGGTTCCCA AGACGATCCC GCTCAGTTGC TGAGAGACCT CCAAGATTCA 
ATGGAACGCG AAGCTGACTT ACGGGAACAA CTGAAATTTG CTGAAGAAGA GATTTGTAAA 
AAATCATGCG GACATTTCAA ATCCCCCGAA TTGCATATTT TTCAACGCAA ATCCCCTTTA 
GTATTGTTTG AGTCTCAAAA CGTGGAGACC AAACTTTTCA CAGTATCGAC CCAAACTTTG 
TTAAATCTTG ACTCATCTGA AGCAGGAACA CAAGCCGCAT TTGATATCTG TGACGCCTCT 
AGTCAAACAA TTGAATGGCG ACCACAAAGT TTCATTTCTT CCATTCAATT CCATCATCTC 
ACAATGTCCA AAATTTACCA ACCAGCGAGA CATTTCTCAC CAATGGCCAC TTTCCTGAAC 
GCAATGGGTC GTAAACTAAG TCCCACCAAT AATTCAAGAT TGACCCCAGA ACCACCTTCT 
GAGAAAGGTG ACGTATCAGA CGACGAAGAC CCCGCAGAAA TCAAATTACA GTTAGAGTTG 
AGCGAACAAG AAGCTTCTGT TCTTCGACGC AAAGTTGAAG ATTTAGAAGC GGAAAACCAT 
AGAATTAAAA CCAAAAACAA GGAACTGCAA GACAAACTTA CCGCGAAAAC TACAACAAAA 
CGCACGGCTG TTGGTGGAGA AAAGGGCACG ACACTGCAAA ATCAGAAGCT GAAAGTTCTA 
GAAGACGAGG CGAACGACCT TCGGAAAAAA CTCATCGAGA AGGAAAGAGA CTGTGAAAGA 
CTACACGCGG AACTTAGTCT AAACCAAAAG AGAAGTAAAA GTGTTCAAAA AAGCAAGTCT 
TTGGATTTGG ATCAGCAAAC TTTGGACTTG AAACGTCAAC TTCAAGTCAT TGAACAAGAA 
GCGAGCATTT TACGAAATAA AATCCAATCG CTTGAGGCAG AGAACGAGAA GTTAATAAGC 
GAGAATAAAA AGCTTCAACT AGTCCGAGGA GCGAAAAACC TCAAATCTGC GGACAAAAAC 
TTAGACAAAT ACATTGACCA AATAGCTTCG TTAGAAATTG AAATTTCCGA GAAAAACGAC 
AAGATCAAAG CGCTGGAAGA AAAATTAGAA ACGGCCCTTA CGCAAGATTC ATCAAGTCTT 
AAAGGAGGAA ATTACAAACG ATTTGTAGAG AGAACACCTA AGAAGGTTTC ACAGTTAACA 
TCGAAGGATC AGTTGAAAAC TATGGTGCAT GATTTGGAGA ATGAAATTGG TGAGATGATT 
GTGGCAATAA AAACCAGCGA AAACGAGAAG ATTAAACTAG AGGAAGAAAT GAAGAAAATG 
CGACACCAGA ACGAAGTCAA TAAAGCGATG CAAGAACTCG AAGAAATGAA CAAGAAATTT 
GAAGAGATGA AGACTGAATT ATCGAAGGAA AAGGAGAAAG TGACTGAAGA GAAAAGTAAA 
TACGACGAAC TTAACAAATC TTTGGTCAAA ACGAAGGAAT CCTTAACGAA ATCGAATCAA 
GAGAAGAAGA AACTTAAAGA ACAAATCGAG AAATCCAAGG AAGAACAGAA GAAAGTACAA 
GAAGAAAAGG ATAAACTGGA CGAAGAAATA GCTAAATTGA AAGCTAATTT AAAAACTGCG 
ACTTATAAAC AAGATGAATT GACCTTAATA AGCCAGAAGG CTGAGTCGCT AAAATTAGAC 
CTTGACTCTA AAGAAAAAGA GTTAAAAACC ATCAAAAAAG AACTTGATAG TAAAATCAAT 
GAGTTATCTG AAAAAGCTTC GAAGGTGTCT CAGTTAGAAC GGAAATTTAG CGAAACTGAA 
GAAAAATTAA AAATTGCAGA AAAACGTGAA AAGGATTTGG AAGCTAAAAT TGAGGAAGAG 
AAGAGTAAAA CCAAGTCGAA AGAAGGAGAG CAAAGTAAAT GGAACGAAGA GCGCAAAAAG 
TACAATAACC AAATTGAGGA ATTAAATAAC AAAATTTTAA GTCTGGAAAC CACAGTTGAG 
AGCAAAAAGA AATTAATCGA GCGATTGGAA GAGAATTTGA AAAAAGAGCG AGAATCGTTT 
TCCAAAGTTG ATGAACTGGA AACTCGCGAA ATCACAAAAT TAAAAGACGA ATTAAGTAAG 
AGCAAAGCTA ATTTGGCCGA CGTTGAGTCC AAACTGGCAT CGTCCCAAAA GAGCCAAAAG 
AATCTCGAAG ATAAGTTGAA AAAATCGGAG ACGGACAGTA AGAATGATAA ACTGTCTCTT 
GAGAAGAAGA AAGGAGAGTT AGAAATTGAA TTACAAAACG AAAAGAAGAA AATCGAGGTG 
ATGAAAGGCA ACCACGAGAA AGAGAACAAA AACAAAGAAA TGGAGCTCGC TTCGCTGAAA 
AGCAAGATAA AATCACTGGA GTTAAACGCC GGAGCTGGGA CGAAAAGACT AGCAGAAATC 
AAACAATTCC AAGAGACTAT AGACAAATTA GAAACTAATC TAAATAAAGA AAAGCAGAAA 
TATGAAGACC TCACGGCTAA GTATGAAATT TTGGAAGAAG AACATGTAGT AACCAAGGCA 
AAGTTGGTTA TGGAGAAAGA AACTATCGAA AATCAACTTT CTAGTACAAG GTCACAACTG 
GACGAATTGG AAGTTGAGTT AAAAACACTG AGAGAAACGT ACAACAAACA ACACGATGAA 
TGGATAAAGG AGAAACTGAG CATGCAAGAA AAAATTAAAG AGATTGAGAA ACGTAACGGA 
AACGGAAGCG AATTAGATAA AGTGCGATAT AAAGCAATGC TGGACGATAA GCAATCCGAG 
TTGGACCAGC TGAGGAAGGA AAATGAGGCG ATGCACGACC AAATGGAATA CATGAGGAAG 
GAGACTGACG AATTGAGGAA AAAACTGGAC GATTACGAAA AAGTGAACAA AGTACAAAGG 
AACATAAGTG CGGATAGTTC GGCAATGGAG AAGGAAATAA GGCAACTTAA AGCAAAGTTG 
AGTTCTATTG AAAAATCCAA AAAGCTCGAA TTGGGCGAGT ACAAAATGCG ATACGACAAC 
CAGCTTTCTA TAGTCAACGG CGAACTGCAA CAGCTCCAAG GCCAAGTGAT GCGGTTCAAG 
CGCGAACGAG ACACGTACAA GCACATGCTA GAGTCGGCTC AGAAAACTAT CGGCGATTTG 
AAAAATTCTC CAAGAAGTGC CAAAGACAAT ACGAATCCTT CTGCACACTA CGATGAGTTG 
GAGGAATCGA AAACAAAAAT TGCAACTCTT GAACAACAAA TCAGTTGCAT GGAGGACGAA 
CTTTCGGAAG CTCGCCTTGA ATGTTCTCGA CTGAAAACCG AACTGGTGTC GGAAAGGTCC 
ACATGGGAAG TGAAAATGTC TGAACTGCAT TCCCGGGTTA ATGAACTAGA AGAGGAGAAA 
ATCCTTAGTA GCGGAAGAAC GAAAATTGTA GGACTGCGGA CTCGAATGGA ACTAGCTTGG 
CAGAAAGAAC GCGAGGAACA GCAGAGATTA TTGCAGGAAA CTGCGACTTT GGCCCGAGAT 
CTTCGGCAGA CGTTGTTCGA AGTTGAGAGG GAACGAGACA AGGAACGATT AGAAGCGAAA 
CGCAAACAAG AACAGTTCAA AAAGTCCTCC GAGGAGGATC AAGACGAAAA TAAGAAGAAA 
ATAACCGAGC TACAGTGCGA TTTGCTGGAA CTAAGAGACG CTCACGCCAA ATTGCGAACC 
ACAAACGAGA AGCTTCGCCG CGAAAAAGAA CGCTACGAGA AAGAAAGAGA AGAATTCAAG 
TCGGTTATTC AAGGCAAGAA GCGCCTGAAC CAAGAAGACG AACGAAAAGT CAACGTCCTG 
ATTGAACAAA TCGACTATTT GAAGCAACTA GCACCAGAAC TGTTCTTCTC CCGCGATAAG 
GAAGCCTCAT ACACTCCAAC TCCCCCACGG AGGAGCAGAA GCTCGAAATC GCGCGAGACT 
TCACCAGCCA TCGAGCGCCG CGAGTCGTCT TTATCACCAG AAGACAAGCA ACAAGAAATC 
CAAAACATGA TGCTTAAACT GGCAAACACT GCGGAAGACC TGCGCCGACT CCAGAAACTA 
ACCGAAGATG AATACGAACG GGAGAGAATC AAGCGCAGTA TGGGGATGAG AAGAGCGACG 
TCCACTGAAC ACGAAAATGT CTCCGAAAGC AGATACAAGT CGTACCAGAA ACGCAAAACC 
GACGGGAGCT TACACCGAAA AAGCTTGTCT CTAGAGCACA CCATAGCCCA GAACGACCCC 
AAAATCTGGA AAAACGACAA CGACAGCATG TCCAGTCTCC ACTCTCTGGA CATGTCTTCA 
GACGTCGAAA GGTGGACAAC GAGGGACACG AGTCTAGACA GTCGCCTCTC TGGTGGTTCC 
ACCCAGAGCG AGTTCGCCGG CGAGAAGAAA AAGAAGAAGG GAATCATGGG CAAGTTGAAG 
AAATTGACCA AATCTCGAAG CATCGATGAC GAAGATCCTG GGACTTTCTC GCCCTCAAGA 
TCACTATCGT CAAAGCAAAA TTCGAGTTCC GAACTTCTGG AGGAGAAAGG GAGCAAGAAG 
GATTTGAGGG AGCGAATCAC GGGTATTTTC AAGAAAAGCG GCTCGTCATC ACGGAGCAAC 
AGTGTGGAAA GACACGAAGT GAGACACGAC ACAAGTTCGA CACACAGGCC CTTGATGCGG 
AATGGCAGTG GCGGGAATTT GTCCTCACGA GCGTCTCCAG CTTTTCAGGA ACCAGAAAAG 
ACACCGAACG CAAAAGCTAA GAGAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015834246.1
CDS	476..6685
Translation	MHHMYPLAKGDPLCPLGFHPQVRWPTRCKRCFRDYKEHGGKRRDEEPSLRRDDTTASTPSLSWSSRDADSERRRSWVSSSNLASDRDNNSVKSESNYSNPSSWTSTPDLAKLEDDTQAVTTVSIKLPKRKQKPIDTGQRTVSDSFSIKPEKSVFSKNDSLAARARKLQQIKEAAEEKHKRIQIKEVKTISEEPTNHDVKRKPTVVEDHHSEPEDDAVSIAGTETTDTTLVDAHDHELREQLESLKKELETTKTKCERLEREKSDILLRRLAAMETTTSKTTASEVLKLQQKCNELQQTLEDFRDEKKSLTFKVKELEEELEQRPTAQAAQKIADELRSKLLAAETLCEELMDENEDIKKELRDMEEQMDEMQDNFREDQAVEYTSLKKELDQTTKNCRILSFKLRKAERKTEQLEQEKNEAERKLKEISGGQSTLANLEKMKQLEQDLKLANEVSIRLQKELDETNQKLQEESKTSKKKAPKLGSIGKFPSADGKVSRESLTRGGSQDDPAQLLRDLQDSMEREADLREQLKFAEEEICKKSCGHFKSPELHIFQRKSPLVLFESQNVETKLFTVSTQTLLNLDSSEAGTQAAFDICDASSQTIEWRPQSFISSIQFHHLTMSKIYQPARHFSPMATFLNAMGRKLSPTNNSRLTPEPPSEKGDVSDDEDPAEIKLQLELSEQEASVLRRKVEDLEAENHRIKTKNKELQDKLTAKTTTKRTAVGGEKGTTLQNQKLKVLEDEANDLRKKLIEKERDCERLHAELSLNQKRSKSVQKSKSLDLDQQTLDLKRQLQVIEQEASILRNKIQSLEAENEKLISENKKLQLVRGAKNLKSADKNLDKYIDQIASLEIEISEKNDKIKALEEKLETALTQDSSSLKGGNYKRFVERTPKKVSQLTSKDQLKTMVHDLENEIGEMIVAIKTSENEKIKLEEEMKKMRHQNEVNKAMQELEEMNKKFEEMKTELSKEKEKVTEEKSKYDELNKSLVKTKESLTKSNQEKKKLKEQIEKSKEEQKKVQEEKDKLDEEIAKLKANLKTATYKQDELTLISQKAESLKLDLDSKEKELKTIKKELDSKINELSEKASKVSQLERKFSETEEKLKIAEKREKDLEAKIEEEKSKTKSKEGEQSKWNEERKKYNNQIEELNNKILSLETTVESKKKLIERLEENLKKERESFSKVDELETREITKLKDELSKSKANLADVESKLASSQKSQKNLEDKLKKSETDSKNDKLSLEKKKGELEIELQNEKKKIEVMKGNHEKENKNKEMELASLKSKIKSLELNAGAGTKRLAEIKQFQETIDKLETNLNKEKQKYEDLTAKYEILEEEHVVTKAKLVMEKETIENQLSSTRSQLDELEVELKTLRETYNKQHDEWIKEKLSMQEKIKEIEKRNGNGSELDKVRYKAMLDDKQSELDQLRKENEAMHDQMEYMRKETDELRKKLDDYEKVNKVQRNISADSSAMEKEIRQLKAKLSSIEKSKKLELGEYKMRYDNQLSIVNGELQQLQGQVMRFKRERDTYKHMLESAQKTIGDLKNSPRSAKDNTNPSAHYDELEESKTKIATLEQQISCMEDELSEARLECSRLKTELVSERSTWEVKMSELHSRVNELEEEKILSSGRTKIVGLRTRMELAWQKEREEQQRLLQETATLARDLRQTLFEVERERDKERLEAKRKQEQFKKSSEEDQDENKKKITELQCDLLELRDAHAKLRTTNEKLRREKERYEKEREEFKSVIQGKKRLNQEDERKVNVLIEQIDYLKQLAPELFFSRDKEASYTPTPPRRSRSSKSRETSPAIERRESSLSPEDKQQEIQNMMLKLANTAEDLRRLQKLTEDEYERERIKRSMGMRRATSTEHENVSESRYKSYQKRKTDGSLHRKSLSLEHTIAQNDPKIWKNDNDSMSSLHSLDMSSDVERWTTRDTSLDSRLSGGSTQSEFAGEKKKKKGIMGKLKKLTKSRSIDDEDPGTFSPSRSLSSKQNSSSELLEEKGSKKDLRERITGIFKKSGSSSRSNSVERHEVRHDTSSTHRPLMRNGSGGNLSSRASPAFQEPEKTPNAKAKRN

Target ORF information:

RefSeq Version	XM_015978760.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum centromere-associated protein E (LOC657022), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015978760.1

ORF Insert Sequence:

ATGCATCACA TGTACCCCCT TGCCAAGGGA GACCCCTTGT GTCCCCTAGG GTTCCACCCC 
CAAGTCAGGT GGCCCACTAG GTGTAAACGG TGCTTCAGAG ACTATAAGGA ACATGGGGGG 
AAGCGAAGGG ACGAAGAACC GTCCCTCCGA AGAGACGACA CCACGGCGTC GACACCTAGT 
CTGTCTTGGA GTTCAAGGGA TGCAGATAGT GAGAGAAGAC GTAGCTGGGT ATCTAGCAGC 
AACTTAGCGT CTGACCGTGA CAATAATTCT GTAAAAAGTG AAAGTAATTA TAGCAATCCA 
TCATCTTGGA CGTCCACTCC AGATTTAGCA AAATTGGAAG ACGATACTCA AGCTGTCACA 
ACAGTTAGCA TAAAATTACC AAAGAGAAAA CAAAAACCGA TCGATACGGG ACAAAGAACT 
GTTTCTGATA GTTTCTCCAT TAAACCCGAA AAGTCAGTAT TTAGTAAAAA CGATAGTTTA 
GCAGCACGCG CTAGGAAATT ACAGCAAATC AAGGAAGCAG CGGAGGAAAA ACACAAACGA 
ATCCAAATAA AAGAAGTCAA AACAATATCA GAGGAACCAA CAAATCACGA TGTGAAACGA 
AAACCTACTG TAGTGGAAGA CCATCATTCA GAGCCCGAAG ACGACGCTGT AAGTATAGCC 
GGAACCGAGA CAACTGACAC CACTTTGGTG GATGCGCACG ATCACGAGTT AAGAGAACAG 
TTAGAGAGTT TAAAAAAAGA GCTAGAAACG ACGAAAACAA AATGTGAAAG ACTTGAACGC 
GAAAAGAGTG ACATTCTCCT GCGAAGACTC GCAGCGATGG AAACAACAAC CAGCAAGACC 
ACTGCTTCGG AAGTCCTGAA ACTCCAGCAA AAGTGTAACG AGTTGCAACA AACTCTCGAG 
GATTTCCGGG ACGAGAAGAA GAGTCTAACT TTTAAGGTGA AGGAGCTGGA GGAGGAGTTG 
GAACAGAGAC CTACGGCCCA AGCGGCGCAG AAAATCGCCG ATGAGCTGCG TTCGAAGCTG 
CTCGCGGCTG AAACACTGTG CGAGGAGCTG ATGGACGAGA ACGAAGACAT CAAGAAGGAG 
CTGAGGGACA TGGAGGAGCA AATGGACGAA ATGCAGGACA ACTTCCGCGA AGACCAGGCC 
GTGGAATACA CCTCACTCAA AAAAGAGTTG GATCAGACCA CCAAAAACTG CAGGATTTTG 
TCGTTCAAGC TGCGAAAAGC CGAACGCAAA ACTGAACAGT TGGAGCAGGA AAAGAACGAA 
GCGGAGCGGA AGTTGAAAGA GATCTCAGGG GGACAGTCAA CCTTGGCCAA TTTGGAGAAA 
ATGAAACAAC TCGAACAGGA TTTGAAGCTA GCTAACGAAG TCTCGATTCG GTTGCAAAAG 
GAGTTGGATG AGACGAATCA AAAGCTTCAA GAGGAGTCCA AGACGAGCAA AAAGAAAGCG 
CCGAAGTTGG GATCCATCGG CAAATTCCCA TCTGCTGATG GAAAAGTTTC CCGCGAGTCT 
CTGACTCGGG GCGGTTCCCA AGACGATCCC GCTCAGTTGC TGAGAGACCT CCAAGATTCA 
ATGGAACGCG AAGCTGACTT ACGGGAACAA CTGAAATTTG CTGAAGAAGA GATTTGTAAA 
AAATCATGCG GACATTTCAA ATCCCCCGAA TTGCATATTT TTCAACGCAA ATCCCCTTTA 
GTATTGTTTG AGTCTCAAAA CGTGGAGACC AAACTTTTCA CAGTATCGAC CCAAACTTTG 
TTAAATCTTG ACTCATCTGA AGCAGGAACA CAAGCCGCAT TTGATATCTG TGACGCCTCT 
AGTCAAACAA TTGAATGGCG ACCACAAAGT TTCATTTCTT CCATTCAATT CCATCATCTC 
ACAATGTCCA AAATTTACCA ACCAGCGAGA CATTTCTCAC CAATGGCCAC TTTCCTGAAC 
GCAATGGGTC GTAAACTAAG TCCCACCAAT AATTCAAGAT TGACCCCAGA ACCACCTTCT 
GAGAAAGGTG ACGTATCAGA CGACGAAGAC CCCGCAGAAA TCAAATTACA GTTAGAGTTG 
AGCGAACAAG AAGCTTCTGT TCTTCGACGC AAAGTTGAAG ATTTAGAAGC GGAAAACCAT 
AGAATTAAAA CCAAAAACAA GGAACTGCAA GACAAACTTA CCGCGAAAAC TACAACAAAA 
CGCACGGCTG TTGGTGGAGA AAAGGGCACG ACACTGCAAA ATCAGAAGCT GAAAGTTCTA 
GAAGACGAGG CGAACGACCT TCGGAAAAAA CTCATCGAGA AGGAAAGAGA CTGTGAAAGA 
CTACACGCGG AACTTAGTCT AAACCAAAAG AGAAGTAAAA GTGTTCAAAA AAGCAAGTCT 
TTGGATTTGG ATCAGCAAAC TTTGGACTTG AAACGTCAAC TTCAAGTCAT TGAACAAGAA 
GCGAGCATTT TACGAAATAA AATCCAATCG CTTGAGGCAG AGAACGAGAA GTTAATAAGC 
GAGAATAAAA AGCTTCAACT AGTCCGAGGA GCGAAAAACC TCAAATCTGC GGACAAAAAC 
TTAGACAAAT ACATTGACCA AATAGCTTCG TTAGAAATTG AAATTTCCGA GAAAAACGAC 
AAGATCAAAG CGCTGGAAGA AAAATTAGAA ACGGCCCTTA CGCAAGATTC ATCAAGTCTT 
AAAGGAGGAA ATTACAAACG ATTTGTAGAG AGAACACCTA AGAAGGTTTC ACAGTTAACA 
TCGAAGGATC AGTTGAAAAC TATGGTGCAT GATTTGGAGA ATGAAATTGG TGAGATGATT 
GTGGCAATAA AAACCAGCGA AAACGAGAAG ATTAAACTAG AGGAAGAAAT GAAGAAAATG 
CGACACCAGA ACGAAGTCAA TAAAGCGATG CAAGAACTCG AAGAAATGAA CAAGAAATTT 
GAAGAGATGA AGACTGAATT ATCGAAGGAA AAGGAGAAAG TGACTGAAGA GAAAAGTAAA 
TACGACGAAC TTAACAAATC TTTGGTCAAA ACGAAGGAAT CCTTAACGAA ATCGAATCAA 
GAGAAGAAGA AACTTAAAGA ACAAATCGAG AAATCCAAGG AAGAACAGAA GAAAGTACAA 
GAAGAAAAGG ATAAACTGGA CGAAGAAATA GCTAAATTGA AAGCTAATTT AAAAACTGCG 
ACTTATAAAC AAGATGAATT GACCTTAATA AGCCAGAAGG CTGAGTCGCT AAAATTAGAC 
CTTGACTCTA AAGAAAAAGA GTTAAAAACC ATCAAAAAAG AACTTGATAG TAAAATCAAT 
GAGTTATCTG AAAAAGCTTC GAAGGTGTCT CAGTTAGAAC GGAAATTTAG CGAAACTGAA 
GAAAAATTAA AAATTGCAGA AAAACGTGAA AAGGATTTGG AAGCTAAAAT TGAGGAAGAG 
AAGAGTAAAA CCAAGTCGAA AGAAGGAGAG CAAAGTAAAT GGAACGAAGA GCGCAAAAAG 
TACAATAACC AAATTGAGGA ATTAAATAAC AAAATTTTAA GTCTGGAAAC CACAGTTGAG 
AGCAAAAAGA AATTAATCGA GCGATTGGAA GAGAATTTGA AAAAAGAGCG AGAATCGTTT 
TCCAAAGTTG ATGAACTGGA AACTCGCGAA ATCACAAAAT TAAAAGACGA ATTAAGTAAG 
AGCAAAGCTA ATTTGGCCGA CGTTGAGTCC AAACTGGCAT CGTCCCAAAA GAGCCAAAAG 
AATCTCGAAG ATAAGTTGAA AAAATCGGAG ACGGACAGTA AGAATGATAA ACTGTCTCTT 
GAGAAGAAGA AAGGAGAGTT AGAAATTGAA TTACAAAACG AAAAGAAGAA AATCGAGGTG 
ATGAAAGGCA ACCACGAGAA AGAGAACAAA AACAAAGAAA TGGAGCTCGC TTCGCTGAAA 
AGCAAGATAA AATCACTGGA GTTAAACGCC GGAGCTGGGA CGAAAAGACT AGCAGAAATC 
AAACAATTCC AAGAGACTAT AGACAAATTA GAAACTAATC TAAATAAAGA AAAGCAGAAA 
TATGAAGACC TCACGGCTAA GTATGAAATT TTGGAAGAAG AACATGTAGT AACCAAGGCA 
AAGTTGGTTA TGGAGAAAGA AACTATCGAA AATCAACTTT CTAGTACAAG GTCACAACTG 
GACGAATTGG AAGTTGAGTT AAAAACACTG AGAGAAACGT ACAACAAACA ACACGATGAA 
TGGATAAAGG AGAAACTGAG CATGCAAGAA AAAATTAAAG AGATTGAGAA ACGTAACGGA 
AACGGAAGCG AATTAGATAA AGTGCGATAT AAAGCAATGC TGGACGATAA GCAATCCGAG 
TTGGACCAGC TGAGGAAGGA AAATGAGGCG ATGCACGACC AAATGGAATA CATGAGGAAG 
GAGACTGACG AATTGAGGAA AAAACTGGAC GATTACGAAA AAGTGAACAA AGTACAAAGG 
AACATAAGTG CGGATAGTTC GGCAATGGAG AAGGAAATAA GGCAACTTAA AGCAAAGTTG 
AGTTCTATTG AAAAATCCAA AAAGCTCGAA TTGGGCGAGT ACAAAATGCG ATACGACAAC 
CAGCTTTCTA TAGTCAACGG CGAACTGCAA CAGCTCCAAG GCCAAGTGAT GCGGTTCAAG 
CGCGAACGAG ACACGTACAA GCACATGCTA GAGTCGGCTC AGAAAACTAT CGGCGATTTG 
AAAAATTCTC CAAGAAGTGC CAAAGACAAT ACGAATCCTT CTGCACACTA CGATGAGTTG 
GAGGAATCGA AAACAAAAAT TGCAACTCTT GAACAACAAA TCAGTTGCAT GGAGGACGAA 
CTTTCGGAAG CTCGCCTTGA ATGTTCTCGA CTGAAAACCG AACTGGTGTC GGAAAGGTCC 
ACATGGGAAG TGAAAATGTC TGAACTGCAT TCCCGGGTTA ATGAACTAGA AGAGGAGAAA 
ATCCTTAGTA GCGGAAGAAC GAAAATTGTA GGACTGCGGA CTCGAATGGA ACTAGCTTGG 
CAGAAAGAAC GCGAGGAACA GCAGAGATTA TTGCAGGAAA CTGCGACTTT GGCCCGAGAT 
CTTCGGCAGA CGTTGTTCGA AGTTGAGAGG GAACGAGACA AGGAACGATT AGAAGCGAAA 
CGCAAACAAG AACAGTTCAA AAAGTCCTCC GAGGAGGATC AAGACGAAAA TAAGAAGAAA 
ATAACCGAGC TACAGTGCGA TTTGCTGGAA CTAAGAGACG CTCACGCCAA ATTGCGAACC 
ACAAACGAGA AGCTTCGCCG CGAAAAAGAA CGCTACGAGA AAGAAAGAGA AGAATTCAAG 
TCGGTTATTC AAGGCAAGAA GCGCCTGAAC CAAGAAGACG AACGAAAAGT CAACGTCCTG 
ATTGAACAAA TCGACTATTT GAAGCAACTA GCACCAGAAC TGTTCTTCTC CCGCGATAAG 
GAAGCCTCAT ACACTCCAAC TCCCCCACGG AGGAGCAGAA GCTCGAAATC GCGCGAGACT 
TCACCAGCCA TCGAGCGCCG CGAGTCGTCT TTATCACCAG AAGACAAGCA ACAAGAAATC 
CAAAACATGA TGCTTAAACT GGCAAACACT GCGGAAGACC TGCGCCGACT CCAGAAACTA 
ACCGAAGATG AATACGAACG GGAGAGAATC AAGCGCAGTA TGGGGATGAG AAGAGCGACG 
TCCACTGAAC ACGAAAATGT CTCCGAAAGC AGATACAAGT CGTACCAGAA ACGCAAAACC 
GACGGGAGCT TACACCGAAA AAGCTTGTCT CTAGAGCACA CCATAGCCCA GAACGACCCC 
AAAATCTGGA AAAACGACAA CGACAGCATG TCCAGTCTCC ACTCTCTGGA CATGTCTTCA 
GACGTCGAAA GGTGGACAAC GAGGGACACG AGTCTAGACA GTCGCCTCTC TGGTGGTTCC 
ACCCAGAGCG AGTTCGCCGG CGAGAAGAAA AAGAAGAAGG GAATCATGGG CAAGTTGAAG 
AAATTGACCA AATCTCGAAG CATCGATGAC GAAGATCCTG GGACTTTCTC GCCCTCAAGA 
TCACTATCGT CAAAGCAAAA TTCGAGTTCC GAACTTCTGG AGGAGAAAGG GAGCAAGAAG 
GATTTGAGGG AGCGAATCAC GGGTATTTTC AAGAAAAGCG GCTCGTCATC ACGGAGCAAC 
AGTGTGGAAA GACACGAAGT GAGACACGAC ACAAGTTCGA CACACAGGCC CTTGATGCGG 
AATGGCAGTG GCGGGAATTT GTCCTCACGA GCGTCTCCAG CTTTTCAGGA ACCAGAAAAG 
ACACCGAACG CAAAAGCTAA GAGAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC657022 cDNA ORF clone, Tribolium castaneum(red flour beetle)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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