TGME49_314955 cDNA ORF clone, Toxoplasma gondii ME49

Gene
Clones
gRNAs

Summary

Gene Symbol	TGME49_314955
Entrez Gene ID	7896037
Full Name	DNA ligase (NAD+), putative
Synonyms	TGME49_114950,TGME49_114960
Gene Type	protein-coding
Organism	Toxoplasma gondii ME49

mRNA and Protein(s)

mRNA	Protein	Name
XM_018782845.1	XP_018635349.1	DNA ligase (NAD+), putative

Pathways from BioSystems
Gene Ontology

The following TGME49_314955 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGME49_314955 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi11635	XM_018782845.1 Latest version!	Toxoplasma gondii ME49 DNA ligase (NAD+), putative mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGME49_314955 ( XM_018782845.1 ) cDNA ORF clone, Toxoplasma gondii ME49 -> XP_018635349.1 Toxoplasma gondii ME49 DNA ligase (NAD+), putative mRNA.

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CloneID	OTi11635
Clone ID Related Accession (Same CDS sequence)	XM_018782845.1
Accession Version	XM_018782845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476806400000
Organism	Toxoplasma gondii ME49
Product	DNA ligase (NAD+), putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_031479).

ATGGGGAAGG GAACGTCTCC ATTACCCGCT CTCTTCCCTC TTCACCCTTG CAGAAGGACC 
TCCTCAGCTT CGCTCTCTCT TTTCCCGCCT GCGTCGTTTC CTCTGATTTT TCCTGTCTTT 
CCTGTCTTCT CCGAGCTTCT CTGCTCTCTC TCGCCTCGAT GCTATTCTCT CCCATCTGCA 
TCTGCAGCTG CTCGCCTGTC TCGTCATCCT CCACGAACGG CTTCCCTCTC TCCATCTCTC 
TGTTCTTCTC TCTGTTCTTC CCTCCCTCCA TCTCTCTGTT CTTCTCTCTC TCCATCTCTC 
TGTTCTTCTC TCTGTTCTTC TCTGTGTTCT TCTCTCTCTC CATCTCTCTG TTCTTCTTCT 
CGCCTGTCTT CTTTCCCCTT CCTGTCGACT TCTTCGCTTC GCTGCGGCGC ATCGTCGTCG 
TCCGTCTCTC CAGCCGCTGG CGTATCTCCC GCGAGTTCTC CTTCTCTTCT TCTTTTCGCC 
TCGTCTCCTC CGTCTCTCGC TGCAGCCTAT GCTCCGCGTC GCCAGCTCCG CTACCGCGCA 
ACGCGCCAGC GTCTCCGTCG CTCACGCGCT CGCAGGGCGG GAGCCGTTGC CTCGCTCCGG 
CTGCGGAGGG CATTCCAGAG CTCGCAACCT GCGCCTGAGT CACGAAGAAC AGGTGCGCTG 
TCGCCGGCTG CCGAACGGCG TCGAGCAGAA GACTCCCGCG ACCGTGTCGA GACCTGCGCT 
GCCGCCTCCG AGAAACGCGA CCCGAGCAGA CAAGCCGCAG GTCGAAGGAA GCCGATTGAC 
GGCTTCGAGG CGAGGGCGGC CGAGCCTGCG TCGGCGCGGA GCGTCGAAGG GCGAAGGACC 
GCGAGTCAGC AGGAGAGGTC CAGAGCGTGG AGGCGTCGCG CCGAGAGCGG CAGAGATGCA 
GAGCGCGAGG CGCGCGGAAG TCGCGCTCTC GAGGAAAAGA GTCCGAGGAG GCGAGAGGCA 
GCGACAGGTT TTCAACCGCG ACTACGACGG AGGGAAGGCG CCTTGGACTC GGAGAAAGAG 
GACTACGCGA CGCTCGTCGA AACTCTCAGA GCGTGCGACC GCGCTTACCA CGTGCATGGA 
TTCTCGCTTC TGCCGGACCC TGTCTACGAC CAACTGAAGA CACGACTGCT GCAGACTGAA 
GAGGCGCATC CTTCCCTGGT GGATCCAAAC AGTCCCTCGT CCTACGTCGG CTCAGGGCCG 
TTCGCGGCTC GCGCCTCACT TGATGACCTC TCGCCTTCGT CGTCGTTGTC AACTTCCTCT 
TTTCCTCTTC CTTCTCCCGC GGTCTGTGCT GAGGCGGATT CCCGGTCTTC TCCGCCGTGT 
GCGTCTTCTC TTTCGTCGAG CGTCTCACCT TCTCTCCTTT CTCCGTCCTC TTCGCTCCTT 
CCTGCGTCTG CCGCTCCTTG CGACGCTCCG CCTTTGTCTG CGTCTCTCGC TCCTTGCGAC 
GCTCCGCCTT TGTCTGCGTC TCTCGCTCCT GGTTCTCGCC TCGATGGCGG ACGCGCGTTT 
CTCCGGCCGA GAGAAGAGCC TCGCGTGCGA CAGCAAACAG GCAGTGTTGG CGTCGAAGCA 
GACGCAGGGA CGCGCGATGC CCGGACGGTG TCGGAGGAGT CCAAAGTCAT TTTCGGAGCT 
CGCGGCAGGA GACGAGTTTA TCACCTTGCC CCCATGTTGT CTCTGTCGGT GGTGCGAGAA 
GGCGCCGCCG ACAGACACCT TCTCTACACG GTCTCTCAAC TCTTCAAGCC TCTTGGACTT 
TCGTGGCTCC CTCGCGAAGC GGTGAAGAAG CGAAACAAAA AAGCAAATGG ATGTTCCTCA 
GCATGCACTA CGTCTGCCAG CATCAAGTCG TCTCCACCTT CTCCCGAGGC ATCGCGGCCT 
ATGCCCCCTC AGACGCCCTT CCCTGTCGCG TTTCTCGCCG AGCCGAAGAT CGATGGCATT 
TCCTGCGCTC TCAGATTCCG CGCTCGCGTC CTTGACTCGG CACTTCGCCG CCCCCCGACG 
CTGCCTCTCG CTGGAGATGT CGTCACTCTC TCAGTCACTG CGTCGGGCCT GTTCGGAGCC 
GTTCCACCCT CTGAAGGGGA AGACGCGCCG GCGTCGCCTG GCGCGAGCGC GTCTCTGGAG 
GACCTTTCCG GAGCGCCTGT TCAGTCAGCC TCTGCGTCGG CTTCGGCTGC TTCGTCGTCG 
CCTTCGACCT TCTCCTCGCT GGTCCTCTGG GATTTCGTCG ACGCCTCGTC TCGGGGAAAT 
GGCGTGTGGG GAGAGGACAT TTCGCACACC GTTCGCGCCA TGAGCGAGGC CGGTCGCCTG 
CCTCTCGCGT TCGCCACAAG GCTTCGCTCC GCTCAACCGC TTTCCCCCGC TACTCTCTCC 
CGCTGGCTTC TCGAGGTTCG CGGCGAATTA TTTCTTTCTC GAAGCAATTT TCGAGCCTAC 
GTCGAACGGC GGCGAAGAGA AGGGAGAACG CCTCCCCTTC ATGAAAGACA TGCGGCAAGA 
GGAATCGCCG ACACCGGCGA GCATGCAGAC CTGCTTTCGT TCGCAGCCTA CGGCCTTGTG 
GAAGGCACAG ATGAGACCGC AAAAGCACAA GTGAATTCTC AGCAGCTCTT CGGGGGAGAA 
ACAGATCCCC TCCCGCTGGC GCCCGCGCCT GGAAGCCATC CAGTCTCGAG TGTATGTACA 
CTTCGAGTTG GCGAGGCGCC GACGCCCGCG TCTGCCATTT CTTCTCGAGT TCCCTATGGC 
GCAGGTTCTG CTTCTCTGGA GAACCAGGCA GGCGGAGACT CTGTGGGCTC TTCGCGCAAC 
GGCGGACCGA CGCTGGTGGC AAGCGTGACT CGACAAGAGT GTGCAGAGAC GCCGAAACGC 
AACGACCTCG CTGCTGTCCA TACACTCGCG ACGCAAGAGG ACCTTCTGGT CCTTCTCAAG 
CGTCTCGGAT TCTGCACGTT TCGAGCTTGG ACGCGAGTCT GTGCGACTGT CGATGAGATT 
CACTCCGCCT TCGCCTTCTT CCACTCGCAC ATCAACGGAG CGAAGGAGCG TAGAGAAGGC 
GAAGAGAACG CGGTGCCGCC TCTGAGGTAT TCGCGCAACC TGGAAGCTGC AGAAGATCTC 
GTCAGAGAGT TCGAAACGCC TCAGAGTACT CTGGTCCCCT TGCCGCCCTC CCCCCTTTCT 
TGTGCTTCCT TCTCGGAGCT CTCGTCATTC ACTCTCCCTC AATCGCTGTC GTCTTCTTCC 
GCCGACACTC CGATCCTCGC TTCACCTTTG TCGTCGTCTG CCTCCTTTCC CCCGCTGTGG 
GATCTCCCGG CGGATGGCGT CGTATTCAAA GTGAATGAGG TCTCTGTGCA GTTGGCGCTG 
GGGAACCGGG CGACAGGCCC TCGGTGGGCG TTCGCGCGCA AGTTTCCGCA GCCATTTGCT 
CTCTCGAAAA TCCGCGACTT CGTCTTCTCC GTCGGCCAGT TCGGCTCCGT CCACGTAGTC 
GCTGCACTGG AACCTGTCCG CTTTGGAAAC CTCACGGTTT GCCGCGCTTC AGTCGGCACC 
CTCGAGACGC TCTCGCGCCG CCAGCTAGCC GTCGGAGACG AGGTGTACGT ACACCTGAGC 
GGGCACACGA CGCCATTGAT TGTCGGAATC AAGGAGGCTC TCGGGGGGTC TCTGGAAGGC 
CGCGGCTCCC GCGCTGGTTG CGAGGCATGG CGTCTGAGGA AGAACGGAGG AGAAACGCGG 
TCCGAGCGAC CACAGGCAGA AGAAGCGAAC GAGAAGGAAA TCTGTTTTCT CTCTGAATCC 
GTCTGCGCGT CCCATTCTCG AAGCCAAGGC ACAGAGGGTG GCACAGCAGG CGTTCAGAGC 
AGACACAGAG AGTGCAATCT GATCGACAAA GAAACCTGTC GAGATCGGAC GTCTGCCTCC 
GGCCTTTTGC TCGGGTCACC TGCAGCAGCT GAGCACACTC CTGGCAGTTT CCCTACCTCT 
CCTGTTCTCT CAACGAGTTC GAGACCTGAA GCGACAGACA ACGCGTCGAG AGCCGAGAGA 
GGCAGCGGAG AAGTCCAGAA ATCAACGGCT TCTCTTCTCC CGAGCAACTT GTCTTGTCCG 
TCCTGTGGAA GTCCTCTTGT GTTTGTTCCC GTGCAACCGA TATCTCCGAA ATCTCGCGAG 
TTTAACTCGA CTAAAGTTCA TTCTTCCGCA TCGCCCTCCT CCAATGGTTC CCCGTCCTCC 
TCTCCCTCTA CTTCTTCTCC ATCTCATTCG ACTGGACCTT CCTATTCTTC TTCCTCGGGG 
TGTCGTCCCG CTGGGCTTTC GTCTGCTTCT CCACTTCCTG CTTCGTCTGC GCCTCTCGAA 
GGAGGTCGCC TTCGTTGCGC TCGCGGCAGA GGCTGTCCTG CTCAAGTTGC GCGTTTGCTG 
CTCCGACTCC TCGACCGCTC TTGTCTGAAT GTGAGGGCTC CTTCAACCTC TTTTCTTTTC 
CACTTGCATG CCAGCGGCCT TCTGCACAGA CCCTCCGACT TTCTGCGCCT CTGTTTTCTC 
TACCACGAGA AGCGGGACCT GATCTTCTCT TCAGGCGAGG CGCCCGCAGC GCAGAAACCT 
GGACGGGGAG AAAAGAGAAG AAATTGCAAG AGGATGGTGG CAGGCCCAAC AGAAGAAGCG 
ACCACGGCCT CTCACCTCAC GTCGGCCTCC CTCTCTGTCG TCAATGGAAG TACTCGACGC 
GAGAACGCAT TTGCACCGCG TTCTGTTGAT CCAAAACTCT CGGCACAGCT CGCGAGACCT 
GTCCATACAC TACAGAGCGT CGACGAGGAA ATTGAACGCC TCCCTTCGCT TTTTGAGGTC 
TGCAACTCGA CCCAAATCCG GTCTCCCCCT GTCGTCCTGA GGAAGACGGC GGCCGACGAC 
CCTCGAATCG TCCCCAAGCT GCTGGGCCTG AAGATGCCTC CTCCGTCTGT CGCTGCAGCC 
CGCCCTGTGG CGCCTCGGCG TATATTCGGA GAAGAACATG TCGAAGCGTA CGCGGCCTCC 
GAGAGCCTGT CGACGCAGCG GAGACACGGA GGGCGCTCGG GGATTCCGCT TCTTCGCGGA 
TGGGAGAGTT TAGTGAAGCG AGTCGTTGCG AGGTGCCAGG AAAGGCTGCC TCTGCATGCA 
TTGATTTTCG CGCTTTCCAT CGAGCGACTC GGACTCCAGG GCGCGCAGCA AATATCCCGC 
GTTTGTGGTG AGGACCTCAC AGGCTTTCTC CACTTTCTCA GGTGGCTTAG GGACTCAGAG 
ACGAGAGGCC AACGTCTCGA ACGAGGAGAA AAGGGAGAGT GCTCAAGTGA TGATGCAGAA 
GCAGAGGGAG AAGGATCGAA AGAAGGACAA GAACAAGACG AGGCCGACGG ACTTGACGGA 
AAGACGAATG GGTTTCGAGG GGAGATCGAA AGCGAAAAAG ACACCAGGTC GTCCGGCCGC 
GAACTCCGCA GGATTGAGGA TGAAGAACTC TCTGTGTCTT GTGCGTCGCG CTCGCTTTTG 
TCTCCACTGA ACTCTGTCGC ATTCTCAGCC GCTTCGCGAT CGTCTCTTTC GTCGTCTTCT 
TCTTCGTCTC TGTCTGTCTC GACATCCCCG CTTGCAGCTT GTCTCGACGG GGACTACGAA 
TCGAATCTCT CTCCCGCTGC TGTTTTCGGA GCGCTTCCAG AGACACCTGC AGAAATGAAG 
CTTTCCTTTC GCTCTCTGCC GGAGTCGCTG CGATCTCGTC TTGCCCTCGT TTTCGCAGAT 
GACGACACGG TCCGCGAGTT GCTTCGGCTC GCTCTCTATT GTGGAGGAGG CATCGCCTCG 
CTTCCTCCGC TTGGTCCCCC TGTCTCCCTC AAGGCGCTCT TCGACTGTTC CTCCACCGCG 
TCTTCCTTTT CGTCGCCTTC TCCACCTCGC GATTCTTGTG GTACCTCCTT CAAGTCTTCC 
GATTCATCCG TCTCTCCTTC CTCTCCTTCC TGCTCCTCCG CTTCTCCTTC CTCTTCCTCT 
GGTTCGTCTT CGCTGTCCCC GCTGGTTGTG CCGGCATCGT CGTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018635349.1
CDS	575..6502
Translation	MGKGTSPLPALFPLHPCRRTSSASLSLFPPASFPLIFPVFPVFSELLCSLSPRCYSLPSASAAARLSRHPPRTASLSPSLCSSLCSSLPPSLCSSLSPSLCSSLCSSLCSSLSPSLCSSSRLSSFPFLSTSSLRCGASSSSVSPAAGVSPASSPSLLLFASSPPSLAAAYAPRRQLRYRATRQRLRRSRARRAGAVASLRLRRAFQSSQPAPESRRTGALSPAAERRRAEDSRDRVETCAAASEKRDPSRQAAGRRKPIDGFEARAAEPASARSVEGRRTASQQERSRAWRRRAESGRDAEREARGSRALEEKSPRRREAATGFQPRLRRREGALDSEKEDYATLVETLRACDRAYHVHGFSLLPDPVYDQLKTRLLQTEEAHPSLVDPNSPSSYVGSGPFAARASLDDLSPSSSLSTSSFPLPSPAVCAEADSRSSPPCASSLSSSVSPSLLSPSSSLLPASAAPCDAPPLSASLAPCDAPPLSASLAPGSRLDGGRAFLRPREEPRVRQQTGSVGVEADAGTRDARTVSEESKVIFGARGRRRVYHLAPMLSLSVVREGAADRHLLYTVSQLFKPLGLSWLPREAVKKRNKKANGCSSACTTSASIKSSPPSPEASRPMPPQTPFPVAFLAEPKIDGISCALRFRARVLDSALRRPPTLPLAGDVVTLSVTASGLFGAVPPSEGEDAPASPGASASLEDLSGAPVQSASASASAASSSPSTFSSLVLWDFVDASSRGNGVWGEDISHTVRAMSEAGRLPLAFATRLRSAQPLSPATLSRWLLEVRGELFLSRSNFRAYVERRRREGRTPPLHERHAARGIADTGEHADLLSFAAYGLVEGTDETAKAQVNSQQLFGGETDPLPLAPAPGSHPVSSVCTLRVGEAPTPASAISSRVPYGAGSASLENQAGGDSVGSSRNGGPTLVASVTRQECAETPKRNDLAAVHTLATQEDLLVLLKRLGFCTFRAWTRVCATVDEIHSAFAFFHSHINGAKERREGEENAVPPLRYSRNLEAAEDLVREFETPQSTLVPLPPSPLSCASFSELSSFTLPQSLSSSSADTPILASPLSSSASFPPLWDLPADGVVFKVNEVSVQLALGNRATGPRWAFARKFPQPFALSKIRDFVFSVGQFGSVHVVAALEPVRFGNLTVCRASVGTLETLSRRQLAVGDEVYVHLSGHTTPLIVGIKEALGGSLEGRGSRAGCEAWRLRKNGGETRSERPQAEEANEKEICFLSESVCASHSRSQGTEGGTAGVQSRHRECNLIDKETCRDRTSASGLLLGSPAAAEHTPGSFPTSPVLSTSSRPEATDNASRAERGSGEVQKSTASLLPSNLSCPSCGSPLVFVPVQPISPKSREFNSTKVHSSASPSSNGSPSSSPSTSSPSHSTGPSYSSSSGCRPAGLSSASPLPASSAPLEGGRLRCARGRGCPAQVARLLLRLLDRSCLNVRAPSTSFLFHLHASGLLHRPSDFLRLCFLYHEKRDLIFSSGEAPAAQKPGRGEKRRNCKRMVAGPTEEATTASHLTSASLSVVNGSTRRENAFAPRSVDPKLSAQLARPVHTLQSVDEEIERLPSLFEVCNSTQIRSPPVVLRKTAADDPRIVPKLLGLKMPPPSVAAARPVAPRRIFGEEHVEAYAASESLSTQRRHGGRSGIPLLRGWESLVKRVVARCQERLPLHALIFALSIERLGLQGAQQISRVCGEDLTGFLHFLRWLRDSETRGQRLERGEKGECSSDDAEAEGEGSKEGQEQDEADGLDGKTNGFRGEIESEKDTRSSGRELRRIEDEELSVSCASRSLLSPLNSVAFSAASRSSLSSSSSSSLSVSTSPLAACLDGDYESNLSPAAVFGALPETPAEMKLSFRSLPESLRSRLALVFADDDTVRELLRLALYCGGGIASLPPLGPPVSLKALFDCSSTASSFSSPSPPRDSCGTSFKSSDSSVSPSSPSCSSASPSSSSGSSSLSPLVVPASSS

Target ORF information:

RefSeq Version	XM_018782845.1
Organism	Toxoplasma gondii ME49
Definition	Toxoplasma gondii ME49 DNA ligase (NAD+), putative mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018782845.1

ORF Insert Sequence:

ATGGGGAAGG GAACGTCTCC ATTACCCGCT CTCTTCCCTC TTCACCCTTG CAGAAGGACC 
TCCTCAGCTT CGCTCTCTCT TTTCCCGCCT GCGTCGTTTC CTCTGATTTT TCCTGTCTTT 
CCTGTCTTCT CCGAGCTTCT CTGCTCTCTC TCGCCTCGAT GCTATTCTCT CCCATCTGCA 
TCTGCAGCTG CTCGCCTGTC TCGTCATCCT CCACGAACGG CTTCCCTCTC TCCATCTCTC 
TGTTCTTCTC TCTGTTCTTC CCTCCCTCCA TCTCTCTGTT CTTCTCTCTC TCCATCTCTC 
TGTTCTTCTC TCTGTTCTTC TCTGTGTTCT TCTCTCTCTC CATCTCTCTG TTCTTCTTCT 
CGCCTGTCTT CTTTCCCCTT CCTGTCGACT TCTTCGCTTC GCTGCGGCGC ATCGTCGTCG 
TCCGTCTCTC CAGCCGCTGG CGTATCTCCC GCGAGTTCTC CTTCTCTTCT TCTTTTCGCC 
TCGTCTCCTC CGTCTCTCGC TGCAGCCTAT GCTCCGCGTC GCCAGCTCCG CTACCGCGCA 
ACGCGCCAGC GTCTCCGTCG CTCACGCGCT CGCAGGGCGG GAGCCGTTGC CTCGCTCCGG 
CTGCGGAGGG CATTCCAGAG CTCGCAACCT GCGCCTGAGT CACGAAGAAC AGGTGCGCTG 
TCGCCGGCTG CCGAACGGCG TCGAGCAGAA GACTCCCGCG ACCGTGTCGA GACCTGCGCT 
GCCGCCTCCG AGAAACGCGA CCCGAGCAGA CAAGCCGCAG GTCGAAGGAA GCCGATTGAC 
GGCTTCGAGG CGAGGGCGGC CGAGCCTGCG TCGGCGCGGA GCGTCGAAGG GCGAAGGACC 
GCGAGTCAGC AGGAGAGGTC CAGAGCGTGG AGGCGTCGCG CCGAGAGCGG CAGAGATGCA 
GAGCGCGAGG CGCGCGGAAG TCGCGCTCTC GAGGAAAAGA GTCCGAGGAG GCGAGAGGCA 
GCGACAGGTT TTCAACCGCG ACTACGACGG AGGGAAGGCG CCTTGGACTC GGAGAAAGAG 
GACTACGCGA CGCTCGTCGA AACTCTCAGA GCGTGCGACC GCGCTTACCA CGTGCATGGA 
TTCTCGCTTC TGCCGGACCC TGTCTACGAC CAACTGAAGA CACGACTGCT GCAGACTGAA 
GAGGCGCATC CTTCCCTGGT GGATCCAAAC AGTCCCTCGT CCTACGTCGG CTCAGGGCCG 
TTCGCGGCTC GCGCCTCACT TGATGACCTC TCGCCTTCGT CGTCGTTGTC AACTTCCTCT 
TTTCCTCTTC CTTCTCCCGC GGTCTGTGCT GAGGCGGATT CCCGGTCTTC TCCGCCGTGT 
GCGTCTTCTC TTTCGTCGAG CGTCTCACCT TCTCTCCTTT CTCCGTCCTC TTCGCTCCTT 
CCTGCGTCTG CCGCTCCTTG CGACGCTCCG CCTTTGTCTG CGTCTCTCGC TCCTTGCGAC 
GCTCCGCCTT TGTCTGCGTC TCTCGCTCCT GGTTCTCGCC TCGATGGCGG ACGCGCGTTT 
CTCCGGCCGA GAGAAGAGCC TCGCGTGCGA CAGCAAACAG GCAGTGTTGG CGTCGAAGCA 
GACGCAGGGA CGCGCGATGC CCGGACGGTG TCGGAGGAGT CCAAAGTCAT TTTCGGAGCT 
CGCGGCAGGA GACGAGTTTA TCACCTTGCC CCCATGTTGT CTCTGTCGGT GGTGCGAGAA 
GGCGCCGCCG ACAGACACCT TCTCTACACG GTCTCTCAAC TCTTCAAGCC TCTTGGACTT 
TCGTGGCTCC CTCGCGAAGC GGTGAAGAAG CGAAACAAAA AAGCAAATGG ATGTTCCTCA 
GCATGCACTA CGTCTGCCAG CATCAAGTCG TCTCCACCTT CTCCCGAGGC ATCGCGGCCT 
ATGCCCCCTC AGACGCCCTT CCCTGTCGCG TTTCTCGCCG AGCCGAAGAT CGATGGCATT 
TCCTGCGCTC TCAGATTCCG CGCTCGCGTC CTTGACTCGG CACTTCGCCG CCCCCCGACG 
CTGCCTCTCG CTGGAGATGT CGTCACTCTC TCAGTCACTG CGTCGGGCCT GTTCGGAGCC 
GTTCCACCCT CTGAAGGGGA AGACGCGCCG GCGTCGCCTG GCGCGAGCGC GTCTCTGGAG 
GACCTTTCCG GAGCGCCTGT TCAGTCAGCC TCTGCGTCGG CTTCGGCTGC TTCGTCGTCG 
CCTTCGACCT TCTCCTCGCT GGTCCTCTGG GATTTCGTCG ACGCCTCGTC TCGGGGAAAT 
GGCGTGTGGG GAGAGGACAT TTCGCACACC GTTCGCGCCA TGAGCGAGGC CGGTCGCCTG 
CCTCTCGCGT TCGCCACAAG GCTTCGCTCC GCTCAACCGC TTTCCCCCGC TACTCTCTCC 
CGCTGGCTTC TCGAGGTTCG CGGCGAATTA TTTCTTTCTC GAAGCAATTT TCGAGCCTAC 
GTCGAACGGC GGCGAAGAGA AGGGAGAACG CCTCCCCTTC ATGAAAGACA TGCGGCAAGA 
GGAATCGCCG ACACCGGCGA GCATGCAGAC CTGCTTTCGT TCGCAGCCTA CGGCCTTGTG 
GAAGGCACAG ATGAGACCGC AAAAGCACAA GTGAATTCTC AGCAGCTCTT CGGGGGAGAA 
ACAGATCCCC TCCCGCTGGC GCCCGCGCCT GGAAGCCATC CAGTCTCGAG TGTATGTACA 
CTTCGAGTTG GCGAGGCGCC GACGCCCGCG TCTGCCATTT CTTCTCGAGT TCCCTATGGC 
GCAGGTTCTG CTTCTCTGGA GAACCAGGCA GGCGGAGACT CTGTGGGCTC TTCGCGCAAC 
GGCGGACCGA CGCTGGTGGC AAGCGTGACT CGACAAGAGT GTGCAGAGAC GCCGAAACGC 
AACGACCTCG CTGCTGTCCA TACACTCGCG ACGCAAGAGG ACCTTCTGGT CCTTCTCAAG 
CGTCTCGGAT TCTGCACGTT TCGAGCTTGG ACGCGAGTCT GTGCGACTGT CGATGAGATT 
CACTCCGCCT TCGCCTTCTT CCACTCGCAC ATCAACGGAG CGAAGGAGCG TAGAGAAGGC 
GAAGAGAACG CGGTGCCGCC TCTGAGGTAT TCGCGCAACC TGGAAGCTGC AGAAGATCTC 
GTCAGAGAGT TCGAAACGCC TCAGAGTACT CTGGTCCCCT TGCCGCCCTC CCCCCTTTCT 
TGTGCTTCCT TCTCGGAGCT CTCGTCATTC ACTCTCCCTC AATCGCTGTC GTCTTCTTCC 
GCCGACACTC CGATCCTCGC TTCACCTTTG TCGTCGTCTG CCTCCTTTCC CCCGCTGTGG 
GATCTCCCGG CGGATGGCGT CGTATTCAAA GTGAATGAGG TCTCTGTGCA GTTGGCGCTG 
GGGAACCGGG CGACAGGCCC TCGGTGGGCG TTCGCGCGCA AGTTTCCGCA GCCATTTGCT 
CTCTCGAAAA TCCGCGACTT CGTCTTCTCC GTCGGCCAGT TCGGCTCCGT CCACGTAGTC 
GCTGCACTGG AACCTGTCCG CTTTGGAAAC CTCACGGTTT GCCGCGCTTC AGTCGGCACC 
CTCGAGACGC TCTCGCGCCG CCAGCTAGCC GTCGGAGACG AGGTGTACGT ACACCTGAGC 
GGGCACACGA CGCCATTGAT TGTCGGAATC AAGGAGGCTC TCGGGGGGTC TCTGGAAGGC 
CGCGGCTCCC GCGCTGGTTG CGAGGCATGG CGTCTGAGGA AGAACGGAGG AGAAACGCGG 
TCCGAGCGAC CACAGGCAGA AGAAGCGAAC GAGAAGGAAA TCTGTTTTCT CTCTGAATCC 
GTCTGCGCGT CCCATTCTCG AAGCCAAGGC ACAGAGGGTG GCACAGCAGG CGTTCAGAGC 
AGACACAGAG AGTGCAATCT GATCGACAAA GAAACCTGTC GAGATCGGAC GTCTGCCTCC 
GGCCTTTTGC TCGGGTCACC TGCAGCAGCT GAGCACACTC CTGGCAGTTT CCCTACCTCT 
CCTGTTCTCT CAACGAGTTC GAGACCTGAA GCGACAGACA ACGCGTCGAG AGCCGAGAGA 
GGCAGCGGAG AAGTCCAGAA ATCAACGGCT TCTCTTCTCC CGAGCAACTT GTCTTGTCCG 
TCCTGTGGAA GTCCTCTTGT GTTTGTTCCC GTGCAACCGA TATCTCCGAA ATCTCGCGAG 
TTTAACTCGA CTAAAGTTCA TTCTTCCGCA TCGCCCTCCT CCAATGGTTC CCCGTCCTCC 
TCTCCCTCTA CTTCTTCTCC ATCTCATTCG ACTGGACCTT CCTATTCTTC TTCCTCGGGG 
TGTCGTCCCG CTGGGCTTTC GTCTGCTTCT CCACTTCCTG CTTCGTCTGC GCCTCTCGAA 
GGAGGTCGCC TTCGTTGCGC TCGCGGCAGA GGCTGTCCTG CTCAAGTTGC GCGTTTGCTG 
CTCCGACTCC TCGACCGCTC TTGTCTGAAT GTGAGGGCTC CTTCAACCTC TTTTCTTTTC 
CACTTGCATG CCAGCGGCCT TCTGCACAGA CCCTCCGACT TTCTGCGCCT CTGTTTTCTC 
TACCACGAGA AGCGGGACCT GATCTTCTCT TCAGGCGAGG CGCCCGCAGC GCAGAAACCT 
GGACGGGGAG AAAAGAGAAG AAATTGCAAG AGGATGGTGG CAGGCCCAAC AGAAGAAGCG 
ACCACGGCCT CTCACCTCAC GTCGGCCTCC CTCTCTGTCG TCAATGGAAG TACTCGACGC 
GAGAACGCAT TTGCACCGCG TTCTGTTGAT CCAAAACTCT CGGCACAGCT CGCGAGACCT 
GTCCATACAC TACAGAGCGT CGACGAGGAA ATTGAACGCC TCCCTTCGCT TTTTGAGGTC 
TGCAACTCGA CCCAAATCCG GTCTCCCCCT GTCGTCCTGA GGAAGACGGC GGCCGACGAC 
CCTCGAATCG TCCCCAAGCT GCTGGGCCTG AAGATGCCTC CTCCGTCTGT CGCTGCAGCC 
CGCCCTGTGG CGCCTCGGCG TATATTCGGA GAAGAACATG TCGAAGCGTA CGCGGCCTCC 
GAGAGCCTGT CGACGCAGCG GAGACACGGA GGGCGCTCGG GGATTCCGCT TCTTCGCGGA 
TGGGAGAGTT TAGTGAAGCG AGTCGTTGCG AGGTGCCAGG AAAGGCTGCC TCTGCATGCA 
TTGATTTTCG CGCTTTCCAT CGAGCGACTC GGACTCCAGG GCGCGCAGCA AATATCCCGC 
GTTTGTGGTG AGGACCTCAC AGGCTTTCTC CACTTTCTCA GGTGGCTTAG GGACTCAGAG 
ACGAGAGGCC AACGTCTCGA ACGAGGAGAA AAGGGAGAGT GCTCAAGTGA TGATGCAGAA 
GCAGAGGGAG AAGGATCGAA AGAAGGACAA GAACAAGACG AGGCCGACGG ACTTGACGGA 
AAGACGAATG GGTTTCGAGG GGAGATCGAA AGCGAAAAAG ACACCAGGTC GTCCGGCCGC 
GAACTCCGCA GGATTGAGGA TGAAGAACTC TCTGTGTCTT GTGCGTCGCG CTCGCTTTTG 
TCTCCACTGA ACTCTGTCGC ATTCTCAGCC GCTTCGCGAT CGTCTCTTTC GTCGTCTTCT 
TCTTCGTCTC TGTCTGTCTC GACATCCCCG CTTGCAGCTT GTCTCGACGG GGACTACGAA 
TCGAATCTCT CTCCCGCTGC TGTTTTCGGA GCGCTTCCAG AGACACCTGC AGAAATGAAG 
CTTTCCTTTC GCTCTCTGCC GGAGTCGCTG CGATCTCGTC TTGCCCTCGT TTTCGCAGAT 
GACGACACGG TCCGCGAGTT GCTTCGGCTC GCTCTCTATT GTGGAGGAGG CATCGCCTCG 
CTTCCTCCGC TTGGTCCCCC TGTCTCCCTC AAGGCGCTCT TCGACTGTTC CTCCACCGCG 
TCTTCCTTTT CGTCGCCTTC TCCACCTCGC GATTCTTGTG GTACCTCCTT CAAGTCTTCC 
GATTCATCCG TCTCTCCTTC CTCTCCTTCC TGCTCCTCCG CTTCTCCTTC CTCTTCCTCT 
GGTTCGTCTT CGCTGTCCCC GCTGGTTGTG CCGGCATCGT CGTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TGME49_314955 cDNA ORF clone, Toxoplasma gondii ME49

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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