DPYD cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	DPYD
Entrez Gene ID	397109
Full Name	dihydropyrimidine dehydrogenase
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214044.2	NP_999209.1	dihydropyrimidine dehydrogenase [NADP(+)] precursor
XM_021088696.1	XP_020944355.1	dihydropyrimidine dehydrogenase [NADP(+)] isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Insights into the mechanism of dihydropyrimidine dehydrogenase from site-directed mutagenesis targeting the active site loop and redox cofactor coordination.
Lohkamp B, Voevodskaya N, Lindqvist Y, Dobritzsch D
Biochimica et biophysica acta1804(12)2198-206(2010 Dec)

Novel disease-causing mutations in the dihydropyrimidine dehydrogenase gene interpreted by analysis of the three-dimensional protein structure.
van Kuilenburg AB, Dobritzsch D, Meinsma R, Haasjes J, Waterham HR, Nowaczyk MJ, Maropoulos GD, Hein G, Kalhoff H, Kirk JM, Baaske H, Aukett A, Duley JA, Ward KP, Lindqvist Y, van Gennip AH
The Biochemical journal364(Pt 1)157-63(2002 May)

Porcine recombinant dihydropyrimidine dehydrogenase: comparison of the spectroscopic and catalytic properties of the wild-type and C671A mutant enzymes.
Rosenbaum K, Jahnke K, Curti B, Hagen WR, Schnackerz KD, Vanoni MA
Biochemistry37(50)17598-609(1998 Dec)

cDNA cloning and chromosome mapping of human dihydropyrimidine dehydrogenase, an enzyme associated with 5-fluorouracil toxicity and congenital thymine uraciluria.
Yokota H, Fernandez-Salguero P, Furuya H, Lin K, McBride OW, Podschun B, Schnackerz KD, Gonzalez FJ
The Journal of biological chemistry269(37)23192-6(1994 Sep)

Comparison of dihydropyrimidine dehydrogenase from human, rat, pig and cow liver. Biochemical and immunological properties.
Lu ZH, Zhang R, Diasio RB
Biochemical pharmacology46(5)945-52(1993 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DPYD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DPYD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe247242	XM_021088696.1 Latest version!	Sus scrofa dihydropyrimidine dehydrogenase (DPYD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSe01234	NM_214044.2 Latest version!	Sus scrofa dihydropyrimidine dehydrogenase (DPYD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DPYD ( XM_021088696.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020944355.1 Sus scrofa dihydropyrimidine dehydrogenase (DPYD), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe247242
Clone ID Related Accession (Same CDS sequence)	XM_021088696.1
Accession Version	XM_021088696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	dihydropyrimidine dehydrogenase [NADP(+)] isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010446.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATCC CACAAATCAG GAATCCTTGT CTGCCATCCC AAGAGAAAAT GCCTGAAGCT 
TATTCTGCAA AGATTGCTCT TTTGGGTGCT GGGCCTGCAA GTATAAGCTG TGCTTCCTTC 
TTGGCTCGAT TAGGCTACTC TGACATCACT ATATTTGAAA AACAAGAATA TGTTGGTGGT 
TTAAGTACTT CTGAAATCCC TCAGTTCCGG CTGCCATATG ATGTAGTGAA TTTTGAGATT 
GAGCTTATGA AGGACCTTGG TGTAAAGATA ATTTGTGGTA AAAGCCTTTC AGAGAATGAA 
ATTACTCTCA ACACTTTAAA AGAAGAAGGG TATAAAGCTG CTTTCATTGG TATAGGTTTG 
CCAGAACCCA AAACGGATGA CATCTTCCAA GGCCTGACAC AGGACCAGGG GTTTTACACA 
TCCAAAGACT TTCTGCCCCT TGTAGCCAAA AGCAGTAAAG CAGGAATGTG CGCCTGTCAC 
TCTCCATTGC CATCGATACG GGGAGCCGTG ATTGTACTCG GAGCTGGAGA CACAGCTTTC 
GACTGTGCAA CATCCGCTTT ACGTTGTGGA GCCCGCCGAG TGTTCCTCGT CTTCAGAAAA 
GGCTTTGTTA ATATAAGAGC TGTCCCTGAG GAGGTGGAGC TTGCTAAGGA AGAAAAATGT 
GAATTTTTGC CTTTCCTGTC CCCACGGAAG GTTATAGTTA AAGGTGGGAG AATTGTTGCC 
GTGCAATTTG TTCGAACAGA ACAAGATGAA ACTGGAAAAT GGAATGAAGA TGAAGATCAG 
ATAGTCCATC TGAAGGCTGA TGTGGTCATC AGTGCCTTTG GCTCAGTGCT GAGGGATCCT 
AAAGTAAAAG AAGCCTTGAG CCCTATAAAA TTTAACAGAT GGGATCTCCC AGAAGTAGAT 
CCAGAAACTA TGCAAACCAG TGAACCATGG GTGTTTGCAG GTGGTGATAT CGTTGGTATG 
GCTAACACTA CGGTGGAATC CGTAAATGAC GGAAAGCAGG CCTCCTGGTA CATTCACAAA 
TATATCCAGG CCCAATATGG AGCTTCAGTT TCTGCCAAGC CCGAACTGCC CCTGTTTTAT 
ACGCCTGTTG ACCTGGTGGA CATCAGTGTG GAAATGGCTG GATTAAAGTT TATAAATCCT 
TTTGGTCTTG CCAGTGCAGC TCCAACTACC AGTTCATCGA TGATTCGAAG AGCTTTTGAA 
GCTGGATGGG GTTTTGCCCT GACCAAAACT TTCTCTCTTG ATAAGGACAT AGTGACAAAT 
GTCTCACCCA GAATCGTCCG GGGGACTACC TCTGGCCCCA TGTACGGCCC TGGACAAAGC 
TCCTTCCTGA ATATTGAGCT CATCAGTGAA AAAACAGCTG CATATTGGTG TCAAAGTGTC 
ACTGAACTAA AAGCTGACTT TCCAGACAAT ATTGTGATCG CCAGCATCAT GTGTAGTTAC 
AACAAAAATG ACTGGATGGA ACTCTCCAGA AAGGCTGAGG CCTCTGGAGC AGATGCCTTG 
GAGTTAAATC TGTCATGTCC ACACGGCATG GGAGAAAGAG GAATGGGCCT GGCTTGTGGG 
CAGGATCCAG AGCTGGTGCG GAACATCTGT CGCTGGGTTA GGCAAGCTGT TCAGATTCCC 
TTTTTTGCCA AGTTGACCCC AAACGTCACT GATATAGTAA GCATCGCCAG AGCGGCCAAG 
GAAGGTGGCG CAGATGGTGT TACAGCCACC AACACGGTCT CAGGTCTCAT GGGATTAAAA 
GCCGATGGCA CGCCCTGGCC AGCGGTGGGT GCTGGCAAGC GGACTACATA CGGAGGAGTG 
TCTGGCACGG CCATCAGACC AATTGCTTTG AGAGCTGTGA CCACCATTGC TCGTGCTTTG 
CCTGGATTTC CCATTTTGGC TACTGGTGGA ATTGACTCAG CTGAAAGTGG ACTTCAGTTT 
CTCCACAGTG GTGCTTCGGT CCTCCAGGTA TGCAGTGCTG TTCAGAATCA GGATTTCACT 
GTCATCCAAG ACTATTGCAC TGGCCTCAAA GCCTTGCTTT ATCTGAAAAG CATTGAAGAA 
CTACAAGGCT GGGATGGGCA GAGTCCAGGT ACCGAGAGTC ACCAGAAGGG GAAACCAGTT 
CCTCGTATTG CTGAACTCAT GGGAAAGAAA CTGCCAAATT TTGGACCTTA TCTGGAGCAA 
CGCAAGAAAA TCATAGCAGA GGAAAAGATG AGACTGAAAG AACAAAATGC AGCTTTTCCA 
CCACTTGAGA GAAAACCTTT TATTCCCAAA AAGCCTATTC CTGCTATTAA GGATGTAATT 
GGAAAAGCAC TGCAGTACCT TGGAACGTTT GGTGAACTGA GCAACATAGA GCAAGTTGTG 
GCTGTGATCG ATGAAGAAAT GTGTATCAAC TGTGGCAAAT GCTACATGAC CTGTAATGAC 
TCTGGCTACC AGGCTATCCA GTTTGATTCC GAAACCCACC TGCCCACCGT TACTGACACT 
TGCACAGGCT GTACCCTGTG TCTCTCCGTC TGCCCTATTA TCGACTGCAT CAGAATGGTT 
TCCAGGACAA CACCTTACGA ACCAAAGAGA GGCTTGCCCT TGGCTGTGAA TCCGGTGTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020944355.1
CDS	236..2818
Translation	MNIPQIRNPCLPSQEKMPEAYSAKIALLGAGPASISCASFLARLGYSDITIFEKQEYVGGLSTSEIPQFRLPYDVVNFEIELMKDLGVKIICGKSLSENEITLNTLKEEGYKAAFIGIGLPEPKTDDIFQGLTQDQGFYTSKDFLPLVAKSSKAGMCACHSPLPSIRGAVIVLGAGDTAFDCATSALRCGARRVFLVFRKGFVNIRAVPEEVELAKEEKCEFLPFLSPRKVIVKGGRIVAVQFVRTEQDETGKWNEDEDQIVHLKADVVISAFGSVLRDPKVKEALSPIKFNRWDLPEVDPETMQTSEPWVFAGGDIVGMANTTVESVNDGKQASWYIHKYIQAQYGASVSAKPELPLFYTPVDLVDISVEMAGLKFINPFGLASAAPTTSSSMIRRAFEAGWGFALTKTFSLDKDIVTNVSPRIVRGTTSGPMYGPGQSSFLNIELISEKTAAYWCQSVTELKADFPDNIVIASIMCSYNKNDWMELSRKAEASGADALELNLSCPHGMGERGMGLACGQDPELVRNICRWVRQAVQIPFFAKLTPNVTDIVSIARAAKEGGADGVTATNTVSGLMGLKADGTPWPAVGAGKRTTYGGVSGTAIRPIALRAVTTIARALPGFPILATGGIDSAESGLQFLHSGASVLQVCSAVQNQDFTVIQDYCTGLKALLYLKSIEELQGWDGQSPGTESHQKGKPVPRIAELMGKKLPNFGPYLEQRKKIIAEEKMRLKEQNAAFPPLERKPFIPKKPIPAIKDVIGKALQYLGTFGELSNIEQVVAVIDEEMCINCGKCYMTCNDSGYQAIQFDSETHLPTVTDTCTGCTLCLSVCPIIDCIRMVSRTTPYEPKRGLPLAVNPVC

Target ORF information:

RefSeq Version	XM_021088696.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa dihydropyrimidine dehydrogenase (DPYD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021088696.1

ORF Insert Sequence:

ATGAATATCC CACAAATCAG GAATCCTTGT CTGCCATCCC AAGAGAAAAT GCCTGAAGCT 
TATTCTGCAA AGATTGCTCT TTTGGGTGCT GGGCCTGCAA GTATAAGCTG TGCTTCCTTC 
TTGGCTCGAT TAGGCTACTC TGACATCACT ATATTTGAAA AACAAGAATA TGTTGGTGGT 
TTAAGTACTT CTGAAATCCC TCAGTTCCGG CTGCCATATG ATGTAGTGAA TTTTGAGATT 
GAGCTTATGA AGGACCTTGG TGTAAAGATA ATTTGTGGTA AAAGCCTTTC AGAGAATGAA 
ATTACTCTCA ACACTTTAAA AGAAGAAGGG TATAAAGCTG CTTTCATTGG TATAGGTTTG 
CCAGAACCCA AAACGGATGA CATCTTCCAA GGCCTGACAC AGGACCAGGG GTTTTACACA 
TCCAAAGACT TTCTGCCCCT TGTAGCCAAA AGCAGTAAAG CAGGAATGTG CGCCTGTCAC 
TCTCCATTGC CATCGATACG GGGAGCCGTG ATTGTACTCG GAGCTGGAGA CACAGCTTTC 
GACTGTGCAA CATCCGCTTT ACGTTGTGGA GCCCGCCGAG TGTTCCTCGT CTTCAGAAAA 
GGCTTTGTTA ATATAAGAGC TGTCCCTGAG GAGGTGGAGC TTGCTAAGGA AGAAAAATGT 
GAATTTTTGC CTTTCCTGTC CCCACGGAAG GTTATAGTTA AAGGTGGGAG AATTGTTGCC 
GTGCAATTTG TTCGAACAGA ACAAGATGAA ACTGGAAAAT GGAATGAAGA TGAAGATCAG 
ATAGTCCATC TGAAGGCTGA TGTGGTCATC AGTGCCTTTG GCTCAGTGCT GAGGGATCCT 
AAAGTAAAAG AAGCCTTGAG CCCTATAAAA TTTAACAGAT GGGATCTCCC AGAAGTAGAT 
CCAGAAACTA TGCAAACCAG TGAACCATGG GTGTTTGCAG GTGGTGATAT CGTTGGTATG 
GCTAACACTA CGGTGGAATC CGTAAATGAC GGAAAGCAGG CCTCCTGGTA CATTCACAAA 
TATATCCAGG CCCAATATGG AGCTTCAGTT TCTGCCAAGC CCGAACTGCC CCTGTTTTAT 
ACGCCTGTTG ACCTGGTGGA CATCAGTGTG GAAATGGCTG GATTAAAGTT TATAAATCCT 
TTTGGTCTTG CCAGTGCAGC TCCAACTACC AGTTCATCGA TGATTCGAAG AGCTTTTGAA 
GCTGGATGGG GTTTTGCCCT GACCAAAACT TTCTCTCTTG ATAAGGACAT AGTGACAAAT 
GTCTCACCCA GAATCGTCCG GGGGACTACC TCTGGCCCCA TGTACGGCCC TGGACAAAGC 
TCCTTCCTGA ATATTGAGCT CATCAGTGAA AAAACAGCTG CATATTGGTG TCAAAGTGTC 
ACTGAACTAA AAGCTGACTT TCCAGACAAT ATTGTGATCG CCAGCATCAT GTGTAGTTAC 
AACAAAAATG ACTGGATGGA ACTCTCCAGA AAGGCTGAGG CCTCTGGAGC AGATGCCTTG 
GAGTTAAATC TGTCATGTCC ACACGGCATG GGAGAAAGAG GAATGGGCCT GGCTTGTGGG 
CAGGATCCAG AGCTGGTGCG GAACATCTGT CGCTGGGTTA GGCAAGCTGT TCAGATTCCC 
TTTTTTGCCA AGTTGACCCC AAACGTCACT GATATAGTAA GCATCGCCAG AGCGGCCAAG 
GAAGGTGGCG CAGATGGTGT TACAGCCACC AACACGGTCT CAGGTCTCAT GGGATTAAAA 
GCCGATGGCA CGCCCTGGCC AGCGGTGGGT GCTGGCAAGC GGACTACATA CGGAGGAGTG 
TCTGGCACGG CCATCAGACC AATTGCTTTG AGAGCTGTGA CCACCATTGC TCGTGCTTTG 
CCTGGATTTC CCATTTTGGC TACTGGTGGA ATTGACTCAG CTGAAAGTGG ACTTCAGTTT 
CTCCACAGTG GTGCTTCGGT CCTCCAGGTA TGCAGTGCTG TTCAGAATCA GGATTTCACT 
GTCATCCAAG ACTATTGCAC TGGCCTCAAA GCCTTGCTTT ATCTGAAAAG CATTGAAGAA 
CTACAAGGCT GGGATGGGCA GAGTCCAGGT ACCGAGAGTC ACCAGAAGGG GAAACCAGTT 
CCTCGTATTG CTGAACTCAT GGGAAAGAAA CTGCCAAATT TTGGACCTTA TCTGGAGCAA 
CGCAAGAAAA TCATAGCAGA GGAAAAGATG AGACTGAAAG AACAAAATGC AGCTTTTCCA 
CCACTTGAGA GAAAACCTTT TATTCCCAAA AAGCCTATTC CTGCTATTAA GGATGTAATT 
GGAAAAGCAC TGCAGTACCT TGGAACGTTT GGTGAACTGA GCAACATAGA GCAAGTTGTG 
GCTGTGATCG ATGAAGAAAT GTGTATCAAC TGTGGCAAAT GCTACATGAC CTGTAATGAC 
TCTGGCTACC AGGCTATCCA GTTTGATTCC GAAACCCACC TGCCCACCGT TACTGACACT 
TGCACAGGCT GTACCCTGTG TCTCTCCGTC TGCCCTATTA TCGACTGCAT CAGAATGGTT 
TCCAGGACAA CACCTTACGA ACCAAAGAGA GGCTTGCCCT TGGCTGTGAA TCCGGTGTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPYD ( NM_214044.2 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999209.1 Sus scrofa dihydropyrimidine dehydrogenase (DPYD), mRNA.

Price & Availability↑

CloneID	OSe01234
Clone ID Related Accession (Same CDS sequence)	NM_214044.2
Accession Version	NM_214044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450800000000
Organism	Sus scrofa(pig)
Product	dihydropyrimidine dehydrogenase [NADP(+)] precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U09179.1. On Jun 28, 2007 this sequence version replaced NM_214044.1. Sequence Note: This sequence has been modified as follows: removed 25 bp suspected to be vector contamination from the 3' end. ##Evidence-Data-START## Transcript exon combination :: U09179.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCCCTG TGCTGAGCAA GGACGTGGCG GACATCGAGA GTATCCTGGC TTTAAATCCT 
CGAACACAGT CTCATGCAGC CCTTCATTCC ACTTTGGCCA AGAAATTGGA TAAGAAACAC 
TGGAAAAGAA ATCCCGATAA GAACTGCTTT CATTGCGAGA AGCTGGAGAA TAATTTTGGT 
GACATCAAGC ACACGACTCT TGGTGAGCGA GGAGCTCTCC GAGAAGCAAT GAGATGCCTG 
AAATGTGCCG ATGCTCCCTG TCAGAAGAGC TGTCCAACTC ATCTAGATAT CAAATCATTC 
ATCACAAGTA TCTCAAATAA GAACTATTAT GGAGCTGCTA AGATGATTTT TTCTGACAAC 
CCTCTTGGTC TGACCTGTGG AATGGTATGT CCAACCTCTG ATCTTTGTGT AGGAGGATGC 
AATTTATATG CAACTGAAGA GGGATCAATT AATATTGGTG GATTGCAGCA GTTTGCTTCT 
GAGGTGTTCA AAGCAATGAA TATCCCACAA ATCAGGAATC CTTGTCTGCC ATCCCAAGAG 
AAAATGCCTG AAGCTTATTC TGCAAAGATT GCTCTTTTGG GTGCTGGGCC TGCAAGTATA 
AGCTGTGCTT CCTTCTTGGC TCGATTAGGC TACTCTGACA TCACTATATT TGAAAAACAA 
GAATATGTTG GTGGTTTAAG TACTTCTGAA ATCCCTCAGT TCCGGCTGCC ATATGATGTA 
GTGAATTTTG AGATTGAGCT TATGAAGGAC CTTGGTGTAA AGATAATTTG TGGTAAAAGC 
CTTTCAGAGA ATGAAATTAC TCTCAACACT TTAAAAGAAG AAGGGTATAA AGCTGCTTTC 
ATTGGTATAG GTTTGCCAGA ACCCAAAACG GATGACATCT TCCAAGGCCT GACACAGGAC 
CAGGGGTTTT ACACATCCAA AGACTTTCTG CCCCTTGTAG CCAAAAGCAG TAAAGCAGGA 
ATGTGTGCCT GTCACTCTCC ATTGCCATCG ATACGGGGAG CCGTGATTGT ACTCGGAGCT 
GGAGACACAG CTTTCGACTG TGCAACATCC GCTTTACGTT GTGGAGCCCG CCGAGTGTTC 
CTCGTCTTCA GAAAAGGCTT TGTTAATATA AGAGCTGTCC CTGAGGAGGT GGAGCTTGCT 
AAGGAAGAAA AATGTGAATT TTTGCCTTTC CTGTCCCCAC GGAAGGTTAT AGTTAAAGGT 
GGGAGAATTG TTGCCGTGCA ATTTGTTCGA ACAGAACAAG ATGAAACTGG AAAATGGAAT 
GAAGATGAAG ATCAGATAGT CCATCTGAAG GCTGATGTGG TCATCAGTGC CTTTGGCTCA 
GTGCTGAGGG ATCCTAAAGT AAAAGAAGCC TTGAGCCCTA TAAAATTTAA CAGATGGGAT 
CTCCCAGAAG TAGATCCAGA AACTATGCAA ACCAGTGAAC CATGGGTGTT TGCAGGTGGT 
GATATCGTTG GTATGGCTAA CACTACGGTG GAATCCGTAA ATGACGGAAA GCAGGCCTCC 
TGGTACATTC ACAAATATAT CCAGGCCCAA TATGGAGCTT CAGTTTCTGC CAAGCCCGAA 
CTGCCCCTGT TTTATACGCC TGTTGACCTG GTGGACATCA GCGTGGAAAT GGCTGGATTA 
AAGTTTATAA ATCCTTTTGG TCTTGCCAGT GCAGCTCCAA CTACCAGTTC ATCGATGATT 
CGAAGAGCTT TTGAAGCTGG ATGGGGTTTT GCCCTGACCA AAACTTTCTC TCTTGATAAG 
GACATAGTGA CAAATGTCTC ACCCAGAATC GTCCGGGGGA CTACCTCTGG CCCCATGTAC 
GGCCCTGGAC AAAGCTCCTT CCTGAATATT GAGCTCATCA GTGAAAAAAC AGCTGCATAT 
TGGTGTCAAA GTGTCACTGA ACTAAAAGCT GACTTTCCAG ACAATATTGT GATCGCCAGC 
ATCATGTGTA GTTACAACAA AAATGACTGG ATGGAACTCT CCAGAAAGGC TGAGGCCTCT 
GGAGCAGATG CCTTGGAGTT AAATCTGTCA TGTCCACACG GCATGGGAGA AAGAGGAATG 
GGCCTGGCTT GTGGGCAGGA TCCAGAGCTG GTGCGGAACA TCTGTCGCTG GGTTAGGCAA 
GCTGTTCAGA TTCCCTTTTT TGCCAAGTTG ACCCCAAACG TCACTGATAT AGTAAGCATC 
GCCAGAGCGG CCAAGGAAGG TGGCGCAGAT GGTGTTACAG CCACCAACAC GGTCTCAGGT 
CTCATGGGAT TAAAAGCCGA TGGCACGCCC TGGCCAGCGG TGGGTGCTGG CAAGCGGACT 
ACATACGGAG GAGTGTCTGG CACGGCCATC AGACCAATTG CTTTGAGAGC TGTGACCACC 
ATTGCTCGTG CTTTGCCTGG ATTTCCCATT TTGGCTACTG GTGGAATTGA CTCAGCTGAA 
AGTGGACTTC AGTTTCTCCA CAGTGGTGCT TCGGTCCTCC AGGTATGCAG TGCTGTTCAG 
AATCAGGATT TCACTGTCAT CCAAGACTAT TGCACTGGCC TCAAAGCCTT GCTTTATCTG 
AAAAGCATTG AAGAACTACA AGGCTGGGAT GGGCAGAGTC CAGGTACCGA GAGTCACCAG 
AAGGGGAAAC CAGTTCCTCG TATTGCTGAA CTCATGGGAA AGAAACTGCC AAATTTTGGA 
CCTTATCTGG AGCAACGCAA GAAAATCATA GCAGAGGAAA AGATGAGACT GAAAGAACAA 
AATGCAGCTT TTCCACCACT TGAGAGAAAA CCTTTTATTC CCAAAAAGCC TATTCCTGCT 
ATTAAGGATG TAATTGGAAA AGCACTGCAG TACCTTGGAA CGTTTGGTGA ACTGAGCAAC 
ATAGAGCAAG TTGTGGCTGT GATCGATGAA GAAATGTGTA TCAACTGTGG CAAATGCTAC 
ATGACCTGTA ATGACTCTGG CTACCAGGCT ATCCAGTTTG ATCCCGAAAC CCACCTGCCC 
ACCGTTACTG ACACTTGCAC AGGCTGTACC CTGTGTCTCT CCGTCTGCCC TATTATCGAC 
TGCATCAGAA TGGTTTCCAG GACAACACCT TACGAACCAA AGAGAGGCTT GCCCTTGGCT 
GTGAATCCGG TGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999209.1
CDS	66..3143
Translation	MAPVLSKDVADIESILALNPRTQSHAALHSTLAKKLDKKHWKRNPDKNCFHCEKLENNFGDIKHTTLGERGALREAMRCLKCADAPCQKSCPTHLDIKSFITSISNKNYYGAAKMIFSDNPLGLTCGMVCPTSDLCVGGCNLYATEEGSINIGGLQQFASEVFKAMNIPQIRNPCLPSQEKMPEAYSAKIALLGAGPASISCASFLARLGYSDITIFEKQEYVGGLSTSEIPQFRLPYDVVNFEIELMKDLGVKIICGKSLSENEITLNTLKEEGYKAAFIGIGLPEPKTDDIFQGLTQDQGFYTSKDFLPLVAKSSKAGMCACHSPLPSIRGAVIVLGAGDTAFDCATSALRCGARRVFLVFRKGFVNIRAVPEEVELAKEEKCEFLPFLSPRKVIVKGGRIVAVQFVRTEQDETGKWNEDEDQIVHLKADVVISAFGSVLRDPKVKEALSPIKFNRWDLPEVDPETMQTSEPWVFAGGDIVGMANTTVESVNDGKQASWYIHKYIQAQYGASVSAKPELPLFYTPVDLVDISVEMAGLKFINPFGLASAAPTTSSSMIRRAFEAGWGFALTKTFSLDKDIVTNVSPRIVRGTTSGPMYGPGQSSFLNIELISEKTAAYWCQSVTELKADFPDNIVIASIMCSYNKNDWMELSRKAEASGADALELNLSCPHGMGERGMGLACGQDPELVRNICRWVRQAVQIPFFAKLTPNVTDIVSIARAAKEGGADGVTATNTVSGLMGLKADGTPWPAVGAGKRTTYGGVSGTAIRPIALRAVTTIARALPGFPILATGGIDSAESGLQFLHSGASVLQVCSAVQNQDFTVIQDYCTGLKALLYLKSIEELQGWDGQSPGTESHQKGKPVPRIAELMGKKLPNFGPYLEQRKKIIAEEKMRLKEQNAAFPPLERKPFIPKKPIPAIKDVIGKALQYLGTFGELSNIEQVVAVIDEEMCINCGKCYMTCNDSGYQAIQFDPETHLPTVTDTCTGCTLCLSVCPIIDCIRMVSRTTPYEPKRGLPLAVNPVC

Target ORF information:

RefSeq Version	NM_214044.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa dihydropyrimidine dehydrogenase (DPYD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214044.2

ORF Insert Sequence:

ATGGCCCCTG TGCTGAGCAA GGACGTGGCG GACATCGAGA GTATCCTGGC TTTAAATCCT 
CGAACACAGT CTCATGCAGC CCTTCATTCC ACTTTGGCCA AGAAATTGGA TAAGAAACAC 
TGGAAAAGAA ATCCCGATAA GAACTGCTTT CATTGCGAGA AGCTGGAGAA TAATTTTGGT 
GACATCAAGC ACACGACTCT TGGTGAGCGA GGAGCTCTCC GAGAAGCAAT GAGATGCCTG 
AAATGTGCCG ATGCTCCCTG TCAGAAGAGC TGTCCAACTC ATCTAGATAT CAAATCATTC 
ATCACAAGTA TCTCAAATAA GAACTATTAT GGAGCTGCTA AGATGATTTT TTCTGACAAC 
CCTCTTGGTC TGACCTGTGG AATGGTATGT CCAACCTCTG ATCTTTGTGT AGGAGGATGC 
AATTTATATG CAACTGAAGA GGGATCAATT AATATTGGTG GATTGCAGCA GTTTGCTTCT 
GAGGTGTTCA AAGCAATGAA TATCCCACAA ATCAGGAATC CTTGTCTGCC ATCCCAAGAG 
AAAATGCCTG AAGCTTATTC TGCAAAGATT GCTCTTTTGG GTGCTGGGCC TGCAAGTATA 
AGCTGTGCTT CCTTCTTGGC TCGATTAGGC TACTCTGACA TCACTATATT TGAAAAACAA 
GAATATGTTG GTGGTTTAAG TACTTCTGAA ATCCCTCAGT TCCGGCTGCC ATATGATGTA 
GTGAATTTTG AGATTGAGCT TATGAAGGAC CTTGGTGTAA AGATAATTTG TGGTAAAAGC 
CTTTCAGAGA ATGAAATTAC TCTCAACACT TTAAAAGAAG AAGGGTATAA AGCTGCTTTC 
ATTGGTATAG GTTTGCCAGA ACCCAAAACG GATGACATCT TCCAAGGCCT GACACAGGAC 
CAGGGGTTTT ACACATCCAA AGACTTTCTG CCCCTTGTAG CCAAAAGCAG TAAAGCAGGA 
ATGTGTGCCT GTCACTCTCC ATTGCCATCG ATACGGGGAG CCGTGATTGT ACTCGGAGCT 
GGAGACACAG CTTTCGACTG TGCAACATCC GCTTTACGTT GTGGAGCCCG CCGAGTGTTC 
CTCGTCTTCA GAAAAGGCTT TGTTAATATA AGAGCTGTCC CTGAGGAGGT GGAGCTTGCT 
AAGGAAGAAA AATGTGAATT TTTGCCTTTC CTGTCCCCAC GGAAGGTTAT AGTTAAAGGT 
GGGAGAATTG TTGCCGTGCA ATTTGTTCGA ACAGAACAAG ATGAAACTGG AAAATGGAAT 
GAAGATGAAG ATCAGATAGT CCATCTGAAG GCTGATGTGG TCATCAGTGC CTTTGGCTCA 
GTGCTGAGGG ATCCTAAAGT AAAAGAAGCC TTGAGCCCTA TAAAATTTAA CAGATGGGAT 
CTCCCAGAAG TAGATCCAGA AACTATGCAA ACCAGTGAAC CATGGGTGTT TGCAGGTGGT 
GATATCGTTG GTATGGCTAA CACTACGGTG GAATCCGTAA ATGACGGAAA GCAGGCCTCC 
TGGTACATTC ACAAATATAT CCAGGCCCAA TATGGAGCTT CAGTTTCTGC CAAGCCCGAA 
CTGCCCCTGT TTTATACGCC TGTTGACCTG GTGGACATCA GCGTGGAAAT GGCTGGATTA 
AAGTTTATAA ATCCTTTTGG TCTTGCCAGT GCAGCTCCAA CTACCAGTTC ATCGATGATT 
CGAAGAGCTT TTGAAGCTGG ATGGGGTTTT GCCCTGACCA AAACTTTCTC TCTTGATAAG 
GACATAGTGA CAAATGTCTC ACCCAGAATC GTCCGGGGGA CTACCTCTGG CCCCATGTAC 
GGCCCTGGAC AAAGCTCCTT CCTGAATATT GAGCTCATCA GTGAAAAAAC AGCTGCATAT 
TGGTGTCAAA GTGTCACTGA ACTAAAAGCT GACTTTCCAG ACAATATTGT GATCGCCAGC 
ATCATGTGTA GTTACAACAA AAATGACTGG ATGGAACTCT CCAGAAAGGC TGAGGCCTCT 
GGAGCAGATG CCTTGGAGTT AAATCTGTCA TGTCCACACG GCATGGGAGA AAGAGGAATG 
GGCCTGGCTT GTGGGCAGGA TCCAGAGCTG GTGCGGAACA TCTGTCGCTG GGTTAGGCAA 
GCTGTTCAGA TTCCCTTTTT TGCCAAGTTG ACCCCAAACG TCACTGATAT AGTAAGCATC 
GCCAGAGCGG CCAAGGAAGG TGGCGCAGAT GGTGTTACAG CCACCAACAC GGTCTCAGGT 
CTCATGGGAT TAAAAGCCGA TGGCACGCCC TGGCCAGCGG TGGGTGCTGG CAAGCGGACT 
ACATACGGAG GAGTGTCTGG CACGGCCATC AGACCAATTG CTTTGAGAGC TGTGACCACC 
ATTGCTCGTG CTTTGCCTGG ATTTCCCATT TTGGCTACTG GTGGAATTGA CTCAGCTGAA 
AGTGGACTTC AGTTTCTCCA CAGTGGTGCT TCGGTCCTCC AGGTATGCAG TGCTGTTCAG 
AATCAGGATT TCACTGTCAT CCAAGACTAT TGCACTGGCC TCAAAGCCTT GCTTTATCTG 
AAAAGCATTG AAGAACTACA AGGCTGGGAT GGGCAGAGTC CAGGTACCGA GAGTCACCAG 
AAGGGGAAAC CAGTTCCTCG TATTGCTGAA CTCATGGGAA AGAAACTGCC AAATTTTGGA 
CCTTATCTGG AGCAACGCAA GAAAATCATA GCAGAGGAAA AGATGAGACT GAAAGAACAA 
AATGCAGCTT TTCCACCACT TGAGAGAAAA CCTTTTATTC CCAAAAAGCC TATTCCTGCT 
ATTAAGGATG TAATTGGAAA AGCACTGCAG TACCTTGGAA CGTTTGGTGA ACTGAGCAAC 
ATAGAGCAAG TTGTGGCTGT GATCGATGAA GAAATGTGTA TCAACTGTGG CAAATGCTAC 
ATGACCTGTA ATGACTCTGG CTACCAGGCT ATCCAGTTTG ATCCCGAAAC CCACCTGCCC 
ACCGTTACTG ACACTTGCAC AGGCTGTACC CTGTGTCTCT CCGTCTGCCC TATTATCGAC 
TGCATCAGAA TGGTTTCCAG GACAACACCT TACGAACCAA AGAGAGGCTT GCCCTTGGCT 
GTGAATCCGG TGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into the mechanism of dihydropyrimidine dehydrogenase from site-directed mutagenesis targeting the active site loop and redox cofactor coordination.
Biochimica et biophysica acta1804(12)2198-206(2010 Dec)
Lohkamp B,Voevodskaya N,Lindqvist Y,Dobritzsch D

Novel disease-causing mutations in the dihydropyrimidine dehydrogenase gene interpreted by analysis of the three-dimensional protein structure.
The Biochemical journal364(Pt 1)157-63(2002 May)
van Kuilenburg AB,Dobritzsch D,Meinsma R,Haasjes J,Waterham HR,Nowaczyk MJ,Maropoulos GD,Hein G,Kalhoff H,Kirk JM,Baaske H,Aukett A,Duley JA,Ward KP,Lindqvist Y,van Gennip AH

Porcine recombinant dihydropyrimidine dehydrogenase: comparison of the spectroscopic and catalytic properties of the wild-type and C671A mutant enzymes.
Biochemistry37(50)17598-609(1998 Dec)
Rosenbaum K,Jahnke K,Curti B,Hagen WR,Schnackerz KD,Vanoni MA

cDNA cloning and chromosome mapping of human dihydropyrimidine dehydrogenase, an enzyme associated with 5-fluorouracil toxicity and congenital thymine uraciluria.
The Journal of biological chemistry269(37)23192-6(1994 Sep)
Yokota H,Fernandez-Salguero P,Furuya H,Lin K,McBride OW,Podschun B,Schnackerz KD,Gonzalez FJ

Comparison of dihydropyrimidine dehydrogenase from human, rat, pig and cow liver. Biochemical and immunological properties.
Biochemical pharmacology46(5)945-52(1993 Sep)
Lu ZH,Zhang R,Diasio RB

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

DPYD cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM

SUPPORT

COMPANY

SUPPORT

COMPANY