VCL cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCL
Entrez Gene ID	396974
Full Name	vinculin
Synonyms	VIN
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 14 Map Location: 14\|14q2.5-q2.9

mRNA and Protein(s)

mRNA	Protein	Name
XM_005671074.2	XP_005671131.1	vinculin isoform X2
XM_005671073.2	XP_005671130.1	vinculin isoform X1
XM_021072078.1	XP_020927737.1	vinculin isoform X1
XM_005671073.3	XP_005671130.1	vinculin isoform X2
XM_005671074.3	XP_005671131.1	vinculin isoform X3
NM_213934.1	NP_999099.1	vinculin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Myosin VI and vinculin cooperate during the morphogenesis of cadherin cell cell contacts in mammalian epithelial cells.
Maddugoda MP, Crampton MS, Shewan AM, Yap AS
The Journal of cell biology178(3)529-40(2007 Jul)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

Variable and constant regions in the C-terminus of vinculin and metavinculin. Cloning and expression of fragments in E. coli.
Strasser P, Gimona M, Herzog M, Geiger B, Small JV
FEBS letters317(3)189-94(1993 Feb)

Porcine vinculin and metavinculin differ by a 68-residue insert located close to the carboxy-terminal part of the molecule.
Gimona M, Small JV, Moeremans M, Van Damme J, Puype M, Vandekerckhove J
The EMBO journal7(8)2329-34(1988 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe00315	NM_213934.1 Latest version!	Sus scrofa vinculin (VCL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe22284	XM_005671073.3 Latest version!	Sus scrofa vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe22285	XM_005671074.3 Latest version!	Sus scrofa vinculin (VCL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe237260	XM_021072078.1 Latest version!	Sus scrofa vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OSe22284	XM_005671073.2	Sus scrofa vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSe22285	XM_005671074.2	Sus scrofa vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCL ( NM_213934.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999099.1 Sus scrofa vinculin (VCL), mRNA.

Price & Availability↑

CloneID	OSe00315
Clone ID Related Accession (Same CDS sequence)	NM_213934.1
Accession Version	NM_213934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573660800000
Organism	Sus scrofa(pig)
Product	vinculin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF165172.1. ##Evidence-Data-START## Transcript exon combination :: AF165172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1876826, SAMEA2041378 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGTGT TTCATACGCG AACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCC TTGGCTTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTTCGTGA CCCCACTGCC TCCCCAGGTG ATGCTGGTGA GCAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTC GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTAGGG CAGATGACTG ATCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAGGGT 
CTGGATGTGC TCACAGCTAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTCGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCCTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGAA AAGGAGATTC CCCAGAGGCC 
CGCGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTGCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATTGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TAGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTGG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAACT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAAGAA GCCATGACTC AAGAGGTGTC AGATGTTTTC 
AGTGATACCA CAACTCCCAT CAAGCTGCTG GCAGTGGCAG CCACTGCCCC TCCTGATGCA 
CCCAATAGAG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCGGCTGCA GTTGGAACTG CTAATAAATC AACAGTAGAA 
GGCATTCAGG CCTCAGTGAA GACAGCCCGA GAACTCACAC CCCAGGTTGT CTCAGCCGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGTTGG TGGACGAAGC CATTGACACC 
AAATCTCTGT TGGATGCTTC CGAAGAAGCG ATTAAAAAAG ACCTGGACAA GTGTAAAGTA 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTTGCTGGGG CAACCAGCAT CGCTCGTCGG 
GCCAACCGCA TTCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCGG TGAAAGCTGC CTCTGACGAA TTGAGCAAAA CCATCTCCCC AATGGTGATG 
GATGCAAAGG CTGTGGCAGG AAACATTTCT GACCCTGGCC TGCAAAAGAG CTTCCTAGAC 
TCAGGATACC GGATCCTGGG AGCCGTGGCC AAGGTGAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC GCCAGACCTT GAACAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCGC CCAGGCCTCC ACCACCAGAG 
GAGAAGGATG AAGAGTTCCC TGAGCAGAAA GCCGGAGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA TGATGAAGCT CGCAAATGGT CCAGTAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGCTA ATGGCCGAGA TGTCTCGGCT GGTCAGAGGG 
GGCAGTGGTA CCAAGCGGGC ACTGATTCAG TGTGCCAAGG ACATCGCCAA GGCCTCAGAC 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG GATTAGAACC 
AACCTCTTAC AGGTATGCGA GCGAATCCCA ACCATAAGCA CCCAGCTTAA AATCCTGTCC 
ACAGTGAAGG CCACCATGCT GGGCCGGACC AACATCAGTG ATGAGGAGTC TGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAT CTGATGCAAT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTAGA ACGGATGCTG GATTTACACT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999099.1
CDS	1..3204
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPTASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	NM_213934.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa vinculin (VCL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213934.1

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG AACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCC TTGGCTTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTTCGTGA CCCCACTGCC TCCCCAGGTG ATGCTGGTGA GCAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTC GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTAGGG CAGATGACTG ATCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAGGGT 
CTGGATGTGC TCACAGCTAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTCGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCCTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGAA AAGGAGATTC CCCAGAGGCC 
CGCGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTGCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATTGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TAGCCGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTGG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAACT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAAGAA GCCATGACTC AAGAGGTGTC AGATGTTTTC 
AGTGATACCA CAACTCCCAT CAAGCTGCTG GCAGTGGCAG CCACTGCCCC TCCTGATGCA 
CCCAATAGAG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCGGCTGCA GTTGGAACTG CTAATAAATC AACAGTAGAA 
GGCATTCAGG CCTCAGTGAA GACAGCCCGA GAACTCACAC CCCAGGTTGT CTCAGCCGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGTTGG TGGACGAAGC CATTGACACC 
AAATCTCTGT TGGATGCTTC CGAAGAAGCG ATTAAAAAAG ACCTGGACAA GTGTAAAGTA 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTTGCTGGGG CAACCAGCAT CGCTCGTCGG 
GCCAACCGCA TTCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCGG TGAAAGCTGC CTCTGACGAA TTGAGCAAAA CCATCTCCCC AATGGTGATG 
GATGCAAAGG CTGTGGCAGG AAACATTTCT GACCCTGGCC TGCAAAAGAG CTTCCTAGAC 
TCAGGATACC GGATCCTGGG AGCCGTGGCC AAGGTGAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC GCCAGACCTT GAACAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCGC CCAGGCCTCC ACCACCAGAG 
GAGAAGGATG AAGAGTTCCC TGAGCAGAAA GCCGGAGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA TGATGAAGCT CGCAAATGGT CCAGTAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGCTA ATGGCCGAGA TGTCTCGGCT GGTCAGAGGG 
GGCAGTGGTA CCAAGCGGGC ACTGATTCAG TGTGCCAAGG ACATCGCCAA GGCCTCAGAC 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG GATTAGAACC 
AACCTCTTAC AGGTATGCGA GCGAATCCCA ACCATAAGCA CCCAGCTTAA AATCCTGTCC 
ACAGTGAAGG CCACCATGCT GGGCCGGACC AACATCAGTG ATGAGGAGTC TGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAT CTGATGCAAT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTAGA ACGGATGCTG GATTTACACT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_005671073.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005671130.1 Sus scrofa vinculin (VCL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe22284
Clone ID Related Accession (Same CDS sequence)	XM_005671073.2 , XM_005671073.3
Accession Version	XM_005671073.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	vinculin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003612019.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005671073.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCCTTG GCTTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TTCGTGACCC CACTGCCTCC CCAGGTGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCTAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTCGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCCTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGAAAAG GAGATTCCCC AGAGGCCCGC 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTGCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTAG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTGGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAACTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAAGAAGCC ATGACTCAAG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGCTGGCA GTGGCAGCCA CTGCCCCTCC TGATGCACCC 
AATAGAGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCTGCAGTT GGAACTGCTA ATAAATCAAC AGTAGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTCACACCCC AGGTTGTCTC AGCCGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGTTGGTGG ACGAAGCCAT TGACACCAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCGATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATCGC TCGTCGGGCC 
AACCGCATTC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCGGTGA AAGCTGCCTC TGACGAATTG AGCAAAACAA TCTCCCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCAGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC CGTGGCCAAG GTGAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCGCC AGACCTTGAA CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCTCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GTAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCTCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAGTCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT AATGGCCGAG 
ATGTCTCGGC TGGTCAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA CGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGCG AGCGAATCCC AACCATAAGC 
ACCCAGCTTA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA TCTGATGCAA 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTAG AACGGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005671130.1
CDS	126..3530
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPTASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAKVGIGVVAEADAADAVGFPVPSDMEDDYEPELLLMPSSQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005671073.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005671073.2

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCCTTG GCTTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TTCGTGACCC CACTGCCTCC CCAGGTGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCTAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTCGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCCTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGAAAAG GAGATTCCCC AGAGGCCCGC 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTGCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTAG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTGGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAACTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAAGAAGCC ATGACTCAAG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGCTGGCA GTGGCAGCCA CTGCCCCTCC TGATGCACCC 
AATAGAGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCTGCAGTT GGAACTGCTA ATAAATCAAC AGTAGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTCACACCCC AGGTTGTCTC AGCCGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGTTGGTGG ACGAAGCCAT TGACACCAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCGATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATCGC TCGTCGGGCC 
AACCGCATTC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCGGTGA AAGCTGCCTC TGACGAATTG AGCAAAACAA TCTCCCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCAGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC CGTGGCCAAG GTGAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCGCC AGACCTTGAA CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCTCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GTAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCTCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAGTCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT AATGGCCGAG 
ATGTCTCGGC TGGTCAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA CGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGCG AGCGAATCCC AACCATAAGC 
ACCCAGCTTA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA TCTGATGCAA 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTAG AACGGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_005671073.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005671130.1 Sus scrofa vinculin (VCL), transcript variant X2, mRNA.

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CloneID	OSe22284
Clone ID Related Accession (Same CDS sequence)	XM_005671073.2 , XM_005671073.3
Accession Version	XM_005671073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005671073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCCTTG GCTTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TTCGTGACCC CACTGCCTCC CCAGGTGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCTAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTCGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCCTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGAAAAG GAGATTCCCC AGAGGCCCGC 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTGCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTAG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTGGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAACTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAAGAAGCC ATGACTCAAG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGCTGGCA GTGGCAGCCA CTGCCCCTCC TGATGCACCC 
AATAGAGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCTGCAGTT GGAACTGCTA ATAAATCAAC AGTAGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTCACACCCC AGGTTGTCTC AGCCGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGTTGGTGG ACGAAGCCAT TGACACCAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCGATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATCGC TCGTCGGGCC 
AACCGCATTC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCGGTGA AAGCTGCCTC TGACGAATTG AGCAAAACAA TCTCCCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCAGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC CGTGGCCAAG GTGAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCGCC AGACCTTGAA CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCTCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GTAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCTCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAGTCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT AATGGCCGAG 
ATGTCTCGGC TGGTCAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA CGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGCG AGCGAATCCC AACCATAAGC 
ACCCAGCTTA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA TCTGATGCAA 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTAG AACGGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005671130.1
CDS	102..3506
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPTASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAKVGIGVVAEADAADAVGFPVPSDMEDDYEPELLLMPSSQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005671073.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005671073.3

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCCTTG GCTTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TTCGTGACCC CACTGCCTCC CCAGGTGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCTAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTCGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCCTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGAAAAG GAGATTCCCC AGAGGCCCGC 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTGCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTAG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTGGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAACTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAAGAAGCC ATGACTCAAG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGCTGGCA GTGGCAGCCA CTGCCCCTCC TGATGCACCC 
AATAGAGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCTGCAGTT GGAACTGCTA ATAAATCAAC AGTAGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTCACACCCC AGGTTGTCTC AGCCGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGTTGGTGG ACGAAGCCAT TGACACCAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCGATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATCGC TCGTCGGGCC 
AACCGCATTC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCGGTGA AAGCTGCCTC TGACGAATTG AGCAAAACAA TCTCCCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCAGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC CGTGGCCAAG GTGAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCGCC AGACCTTGAA CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCTCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GTAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAGGTGT TGTAGCTGAG GCAGATGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCTCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAGTCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGCT AATGGCCGAG 
ATGTCTCGGC TGGTCAGAGG GGGCAGTGGT ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA CGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTCTTA CAGGTATGCG AGCGAATCCC AACCATAAGC 
ACCCAGCTTA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GATGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA TCTGATGCAA 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTAG AACGGATGCT 
GGATTTACAC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_005671074.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005671131.1 Sus scrofa vinculin (VCL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe22285
Clone ID Related Accession (Same CDS sequence)	XM_005671074.2 , XM_005671074.3
Accession Version	XM_005671074.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	vinculin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003612019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005671074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCCTTG GCTTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TTCGTGACCC CACTGCCTCC CCAGGTGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCTAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTCGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCCTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGAAAAG GAGATTCCCC AGAGGCCCGC 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTGCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTAG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTGGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAACTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAAGAAGCC ATGACTCAAG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGCTGGCA GTGGCAGCCA CTGCCCCTCC TGATGCACCC 
AATAGAGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCTGCAGTT GGAACTGCTA ATAAATCAAC AGTAGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTCACACCCC AGGTTGTCTC AGCCGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGTTGGTGG ACGAAGCCAT TGACACCAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCGATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATCGC TCGTCGGGCC 
AACCGCATTC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCGGTGA AAGCTGCCTC TGACGAATTG AGCAAAACAA TCTCCCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCAGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC CGTGGCCAAG GTGAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCGCC AGACCTTGAA CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCTCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GTAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTAATG GCCGAGATGT CTCGGCTGGT CAGAGGGGGC 
AGTGGTACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGACGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTCTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTTAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAATCTG ATGCAATCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTAGAACG GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005671131.1
CDS	1..3201
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPTASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005671074.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005671074.2

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCCTTG GCTTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TTCGTGACCC CACTGCCTCC CCAGGTGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCTAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTCGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCCTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGAAAAG GAGATTCCCC AGAGGCCCGC 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTGCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTAG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTGGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAACTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAAGAAGCC ATGACTCAAG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGCTGGCA GTGGCAGCCA CTGCCCCTCC TGATGCACCC 
AATAGAGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCTGCAGTT GGAACTGCTA ATAAATCAAC AGTAGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTCACACCCC AGGTTGTCTC AGCCGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGTTGGTGG ACGAAGCCAT TGACACCAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCGATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATCGC TCGTCGGGCC 
AACCGCATTC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCGGTGA AAGCTGCCTC TGACGAATTG AGCAAAACAA TCTCCCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCAGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC CGTGGCCAAG GTGAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCGCC AGACCTTGAA CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCTCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GTAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTAATG GCCGAGATGT CTCGGCTGGT CAGAGGGGGC 
AGTGGTACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGACGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTCTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTTAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAATCTG ATGCAATCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTAGAACG GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_005671074.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_005671131.1 Sus scrofa vinculin (VCL), transcript variant X3, mRNA.

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CloneID	OSe22285
Clone ID Related Accession (Same CDS sequence)	XM_005671074.2 , XM_005671074.3
Accession Version	XM_005671074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	vinculin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005671074.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCCTTG GCTTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TTCGTGACCC CACTGCCTCC CCAGGTGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCTAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTCGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCCTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGAAAAG GAGATTCCCC AGAGGCCCGC 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTGCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTAG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTGGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAACTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAAGAAGCC ATGACTCAAG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGCTGGCA GTGGCAGCCA CTGCCCCTCC TGATGCACCC 
AATAGAGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCTGCAGTT GGAACTGCTA ATAAATCAAC AGTAGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTCACACCCC AGGTTGTCTC AGCCGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGTTGGTGG ACGAAGCCAT TGACACCAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCGATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATCGC TCGTCGGGCC 
AACCGCATTC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCGGTGA AAGCTGCCTC TGACGAATTG AGCAAAACAA TCTCCCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCAGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC CGTGGCCAAG GTGAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCGCC AGACCTTGAA CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCTCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GTAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTAATG GCCGAGATGT CTCGGCTGGT CAGAGGGGGC 
AGTGGTACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGACGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTCTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTTAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAATCTG ATGCAATCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTAGAACG GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005671131.1
CDS	145..3345
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPTASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005671074.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa vinculin (VCL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005671074.3

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCCTTG GCTTCCATAG ACTCCAAACT GAACCAGGCC 
AAAGGTTGGC TTCGTGACCC CACTGCCTCC CCAGGTGATG CTGGTGAGCA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTCGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTAGGGCAG ATGACTGATC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAGGTGTC TCAGGGTCTG 
GATGTGCTCA CAGCTAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCGAAGAA GATCGATGCT GCTCAGAATT GGCTCGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCCTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGCAGA TCTACGAAGA CAGGGAAAAG GAGATTCCCC AGAGGCCCGC 
GCATTGGCCA AACAGGTGGC CACGGCCCTG CAGAACTTGC AGACCAAAAC CAACAGGGCT 
GTGGCCAACA GCAGACCTGC CAAAGCAGCT GTGCACCTTG AGGGCAAGAT TGAGCAAGCA 
CAGCGGTGGA TTGATAATCC CACAGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGG 
GGGCTTGTAG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAAGAT 
CTTCTGGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGTCC TCAGGCGAGA GCACTTGCAT CTCAACTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAAGAAGCC ATGACTCAAG AGGTGTCAGA TGTTTTCAGT 
GATACCACAA CTCCCATCAA GCTGCTGGCA GTGGCAGCCA CTGCCCCTCC TGATGCACCC 
AATAGAGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGTGCCACAG CAGAGAAGGC GGCTGCAGTT GGAACTGCTA ATAAATCAAC AGTAGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCCCGAGAA CTCACACCCC AGGTTGTCTC AGCCGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA AAAAATGACA GGGTTGGTGG ACGAAGCCAT TGACACCAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCGATT AAAAAAGACC TGGACAAGTG TAAAGTAGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTT GCTGGGGCAA CCAGCATCGC TCGTCGGGCC 
AACCGCATTC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAGTTCCGT 
GAGGCGGTGA AAGCTGCCTC TGACGAATTG AGCAAAACAA TCTCCCCAAT GGTGATGGAT 
GCAAAGGCTG TGGCAGGAAA CATTTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTGGGAGC CGTGGCCAAG GTGAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCGCC AGACCTTGAA CAGCTCCGCC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCGCCCA GGCCTCCACC ACCAGAGGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGAGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCATGA TGAAGCTCGC AAATGGTCCA GTAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGCTAATG GCCGAGATGT CTCGGCTGGT CAGAGGGGGC 
AGTGGTACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGACGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTCTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTTAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGATG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAATCTG ATGCAATCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTAGAACG GATGCTGGAT TTACACTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_021072078.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020927737.1 Sus scrofa vinculin (VCL), transcript variant X1, mRNA.

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CloneID	OSe237260
Clone ID Related Accession (Same CDS sequence)	XM_021072078.1
Accession Version	XM_021072078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010456.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCC TTGGCTTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTTCGTGA CCCCACTGCC TCCCCAGGTG ATGCTGGTGA GCAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTC GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTAGGG CAGATGACTG ATCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAGGGT 
CTGGATGTGC TCACAGCTAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTCGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCCTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGAA AAGGAGATTC CCCAGAGGCC 
CGCGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTGCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATTGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TAGCTGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTGG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAACT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAAGAA GCCATGACTC AAGAGGTGTC AGATGTTTTC 
AGTGATACCA CAACTCCCAT CAAGCTGCTG GCAGTGGCAG CCACTGCCCC TCCTGATGCA 
CCCAATAGAG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCGGCTGCA GTTGGAACTG CTAATAAATC AACAGTAGAA 
GGCATTCAGG CCTCAGTGAA GACAGCCCGA GAACTCACAC CCCAGGTTGT CTCAGCCGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGTTGG TGGACGAAGC CATTGACACC 
AAATCTCTGT TGGATGCTTC CGAAGAAGCG ATTAAAAAAG ACCTGGACAA GTGTAAAGTA 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTTGCTGGGG CAACCAGCAT CGCTCGTCGG 
GCCAACCGCA TTCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCGG TGAAAGCTGC CTCTGACGAA TTGAGCAAAA CAATCTCCCC AATGGTGATG 
GATGCAAAGG CTGTGGCAGG AAACATTTCT GACCCTGGCC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTGGG AGCCGTGGCC AAGGTGAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC GCCAGACCTT GAACAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCGC CCAGGCCTCC ACCACCAGAG 
GAGAAGGATG AAGAGTTCCC TGAGCAGAAA GCCGGAGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA TGATGAAGCT CGCAAATGGT CCAGTAAGCC GGGTAACCCA 
GCCGCTAAGG TGGGTATAGG TGTTGTAGCT GAGGCAGATG CGGCTGATGC TGTTGGGTTC 
CCTGTCCCCT CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAGT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GCTAATGGCC 
GAGATGTCTC GGCTGGTCAG AGGGGGCAGT GGTACCAAGC GGGCACTGAT TCAGTGTGCC 
AAGGACATCG CCAAGGCCTC AGACGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGGATTAG AACCAACCTC TTACAGGTAT GCGAGCGAAT CCCAACCATA 
AGCACCCAGC TTAAAATCCT GTCCACAGTG AAGGCCACCA TGCTGGGCCG GACCAACATC 
AGTGATGAGG AGTCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAATCTGATG 
CAATCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TAGAACGGAT 
GCTGGATTTA CACTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020927737.1
CDS	102..3509
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPTASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAKVGIGVVAEADAADAVGFPVPSDMEDDYEPELLLMPSSQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_021072078.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021072078.1

ORF Insert Sequence:

ATGCCGGTGT TTCATACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATCATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTCCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTTGAGAATG CTTGTACCAA GCTTGTCCAG GCAGCCCAGA TGCTTCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGACTA TCTGATCGAT GGGTCAAGGG GCATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA TGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAAGTG GTGGAAACTA TGGAAGATTT GGTCACTTAC 
ACAAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAGA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ACCAGGAGCA CCGAGTGATG TTGGTGAATT CAATGAACAC TGTAAAAGAG 
CTGTTGCCAG TTCTCATTTC CGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGTGCAGAA 
ATTAATGAAA TCATTCGTGT ATTACAACTC ACTTCTTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCC TTGGCTTCCA TAGACTCCAA ACTGAACCAG 
GCCAAAGGTT GGCTTCGTGA CCCCACTGCC TCCCCAGGTG ATGCTGGTGA GCAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTC GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTAGGG CAGATGACTG ATCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAGGT GTCTCAGGGT 
CTGGATGTGC TCACAGCTAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCGAA GAAGATCGAT GCTGCTCAGA ATTGGCTCGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCCTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGC AGATCTACGA AGACAGGGAA AAGGAGATTC CCCAGAGGCC 
CGCGCATTGG CCAAACAGGT GGCCACGGCC CTGCAGAACT TGCAGACCAA AACCAACAGG 
GCTGTGGCCA ACAGCAGACC TGCCAAAGCA GCTGTGCACC TTGAGGGCAA GATTGAGCAA 
GCACAGCGGT GGATTGATAA TCCCACAGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGGGGGCTTG TAGCTGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAA 
GATCTTCTGG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG TCCTCAGGCG AGAGCACTTG CATCTCAACT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAAGAA GCCATGACTC AAGAGGTGTC AGATGTTTTC 
AGTGATACCA CAACTCCCAT CAAGCTGCTG GCAGTGGCAG CCACTGCCCC TCCTGATGCA 
CCCAATAGAG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGTGCCA CAGCAGAGAA GGCGGCTGCA GTTGGAACTG CTAATAAATC AACAGTAGAA 
GGCATTCAGG CCTCAGTGAA GACAGCCCGA GAACTCACAC CCCAGGTTGT CTCAGCCGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TTGATAATGT TGAAAAAATG ACAGGGTTGG TGGACGAAGC CATTGACACC 
AAATCTCTGT TGGATGCTTC CGAAGAAGCG ATTAAAAAAG ACCTGGACAA GTGTAAAGTA 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTTGCTGGGG CAACCAGCAT CGCTCGTCGG 
GCCAACCGCA TTCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAGTTC 
CGTGAGGCGG TGAAAGCTGC CTCTGACGAA TTGAGCAAAA CAATCTCCCC AATGGTGATG 
GATGCAAAGG CTGTGGCAGG AAACATTTCT GACCCTGGCC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTGGG AGCCGTGGCC AAGGTGAGAG AAGCCTTCCA ACCTCAGGAG 
CCTGACTTCC CGCCTCCTCC GCCAGACCTT GAACAGCTCC GCCTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCGC CCAGGCCTCC ACCACCAGAG 
GAGAAGGATG AAGAGTTCCC TGAGCAGAAA GCCGGAGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA TGATGAAGCT CGCAAATGGT CCAGTAAGCC GGGTAACCCA 
GCCGCTAAGG TGGGTATAGG TGTTGTAGCT GAGGCAGATG CGGCTGATGC TGTTGGGTTC 
CCTGTCCCCT CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAGT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GCTAATGGCC 
GAGATGTCTC GGCTGGTCAG AGGGGGCAGT GGTACCAAGC GGGCACTGAT TCAGTGTGCC 
AAGGACATCG CCAAGGCCTC AGACGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGGATTAG AACCAACCTC TTACAGGTAT GCGAGCGAAT CCCAACCATA 
AGCACCCAGC TTAAAATCCT GTCCACAGTG AAGGCCACCA TGCTGGGCCG GACCAACATC 
AGTGATGAGG AGTCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAATCTGATG 
CAATCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TAGAACGGAT 
GCTGGATTTA CACTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Myosin VI and vinculin cooperate during the morphogenesis of cadherin cell cell contacts in mammalian epithelial cells.
The Journal of cell biology178(3)529-40(2007 Jul)
Maddugoda MP,Crampton MS,Shewan AM,Yap AS

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T

Variable and constant regions in the C-terminus of vinculin and metavinculin. Cloning and expression of fragments in E. coli.
FEBS letters317(3)189-94(1993 Feb)
Strasser P,Gimona M,Herzog M,Geiger B,Small JV

Porcine vinculin and metavinculin differ by a 68-residue insert located close to the carboxy-terminal part of the molecule.
The EMBO journal7(8)2329-34(1988 Aug)
Gimona M,Small JV,Moeremans M,Van Damme J,Puype M,Vandekerckhove J

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VCL cDNA ORF clone, Sus scrofa(pig)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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