FTO cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	FTO
Entrez Gene ID	100127165
Full Name	FTO, alpha-ketoglutarate dependent dioxygenase
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 6 Map Location: 6\|6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001112692.1	NP_001106162.1	alpha-ketoglutarate-dependent dioxygenase FTO

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

FTO Promotes Adipogenesis through Inhibition of the Wnt/β-catenin Signaling Pathway in Porcine Intramuscular Preadipocytes.
Chen X, Luo Y, Jia G, Liu G, Zhao H, Huang Z
Animal biotechnology28(4)268-274(2017 Oct)

Nucleotide variability and haplotype heterogeneity at the porcine fat mass and obesity-associated (FTO) gene.
Fontanesi L, Russo V
Animal genetics44(1)96-100(2013 Feb)

Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses.
Dvořáková V, Bartenschlager H, Stratil A, Horák P, Stupka R, Cítek J, Sprysl M, Hrdlicová A, Geldermann H
Genetics, selection, evolution : GSE4413(2012 Apr)

Splice variant identification and expression analysis of the fat mass and obesity-associated (FTO) gene in intact and castrated male pigs.
Huang J, Liu G, Liu Y, Yao Y, Wu K, Fang M
DNA and cell biology29(12)729-33(2010 Dec)

Expression studies of the obesity candidate gene FTO in pig.
Madsen MB, Birck MM, Fredholm M, Cirera S
Animal biotechnology21(1)51-63(2010)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FTO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FTO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00753	NM_001112692.1 Latest version!	Sus scrofa FTO alpha-ketoglutarate dependent dioxygenase (FTO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FTO ( NM_001112692.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001106162.1 Sus scrofa FTO alpha-ketoglutarate dependent dioxygenase (FTO), mRNA.

Price & Availability↑

CloneID	OSe00753
Clone ID Related Accession (Same CDS sequence)	NM_001112692.1
Accession Version	NM_001112692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548604800000
Organism	Sus scrofa(pig)
Product	alpha-ketoglutarate-dependent dioxygenase FTO
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU249758.1. ##Evidence-Data-START## Transcript exon combination :: EU249758.1 [ECO:0000332] ##Evidence-Data-END##

ATGAAGCGAA CCCCAACCGC CGAGGAACGA GAGCGCGGAG CTAAGAAACT GAGGCTTCTT 
GAAGAGCTGG AAGACACTTG GCTTCCTTAT CTGACCCCCA AAGATGATGA ATTCTATCAG 
CAGTGGCAGC TGAAATACCC TAAGCTAATT CTCCGAGAAG CAGGCAGCGT CCCTGAGGGA 
CTCCACAAAG AGGTTCAAGA AGCCTTCCTC GCACTGCACA AGCATGGCTG CTTATTTCGG 
GACCTGGTCA GGATCCAAGG CAAAGATTTG CTCACGCCAG TATCTCGCCT CCTCATTGGT 
AACCCCGGCT GCACCTACAA GTACCTGAAC ACCAGGCTCT TCACGGTCCC CTGGCCAGTG 
AAGGGCTCTG ATGCAAAGTA CAATGAGGCC GAGATAGGCG CCGCCTGCCA GACCTTCCTC 
AAGCTCAACG ACTACCTGCA GATTGAGACC ATCCAGGCGC TGGAGGAACT CGCTGCCAAG 
GAGAAAGCCA ATATCGACAC CGTGCCGGCG TGTATAGGTC CAGATTTCCC CAGGGTCGGC 
ATGGGGTCAT CCTTTGACGG CCATGACGAG GTGGACAGGA AGAGCAGAGC CGCCTACAAC 
CTAACTTTGT TGAGCTTCAT GGATCCCCAG AAAATGCCGT ACCTGAAAGA GGAGCCCTAC 
TTTGGCATGG GGAAGATGGC TGTGAGCTGG CATCACGATG AAAATCTGGT GGACAGGTCA 
GCGGTGGCAG TGTACAATTA TAGCTGTGAA GGCCCTGAAG AGGAAAGCGA GGATGATCCC 
CAGCTCGAAG GCAGAAATCC CGATGTGTGG CATGTTGGCT TTAAGATCTC ATGGGACATA 
GAGACCCCTG GTTTGGCGAT ACCCCTTCAC CAAGGAGACT GCTACTTTAT GCTGGATGAT 
CTCAATGCCA CCCACCAACA CTGTGTTTTG GCTGGTTTAC CACCCCGGTT TAGTTCCACC 
CACCGAGTGG CCGAGTGCTC GACGGGAGCC TTGGATTACA TCTTACAGCG CTGCCAGTTG 
GCCCTGCAGA ATGTCCGTGA TGAGGCGGAC AGTGGTGAAG TCTCTTTGAA ATCCTTGGAG 
CCTGCGGTTT TGAAACAAGG AGAAGAAATC CACAACGAGG TCGAGTTTGA GTGGCTGAGA 
CAGTTTTGGT TTCAAGGCAA TCGATACAAA AAGTGCACCG ATTGGTGGTG TCAACCCATG 
ACTCAGCTGG AAGAGCTTTG GAAGAAGATG GAAGGTGCGA CCCATGCTGT GCTTCGTGAA 
GTTAGAAGAG AGGGGGCCCC TGTGGAACAG AGCAGTGACA TCCTGACTGC CATCCTAGCC 
GTGCTCACCA CTCGCCAGAA CCTGAGGAGG GAGTGGCATG CCAGGTGCCA GTCCCGAATT 
GCCCGAACTC TGCCTGTGGA CCAGAAGCCA GAATGCCGGC CGTATTGGGA AAAGGATGAT 
CCCTCCATGC CTCTGCCGTT TGATCTCACA GACACTGTGG CTGAACTCAG AGGTCTGCTT 
CTGGAAGCCA AACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106162.1
CDS	15..1532
Translation	MKRTPTAEERERGAKKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREAGSVPEGLHKEVQEAFLALHKHGCLFRDLVRIQGKDLLTPVSRLLIGNPGCTYKYLNTRLFTVPWPVKGSDAKYNEAEIGAACQTFLKLNDYLQIETIQALEELAAKEKANIDTVPACIGPDFPRVGMGSSFDGHDEVDRKSRAAYNLTLLSFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYNYSCEGPEEESEDDPQLEGRNPDVWHVGFKISWDIETPGLAIPLHQGDCYFMLDDLNATHQHCVLAGLPPRFSSTHRVAECSTGALDYILQRCQLALQNVRDEADSGEVSLKSLEPAVLKQGEEIHNEVEFEWLRQFWFQGNRYKKCTDWWCQPMTQLEELWKKMEGATHAVLREVRREGAPVEQSSDILTAILAVLTTRQNLRREWHARCQSRIARTLPVDQKPECRPYWEKDDPSMPLPFDLTDTVAELRGLLLEAKP

Target ORF information:

RefSeq Version	NM_001112692.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa FTO alpha-ketoglutarate dependent dioxygenase (FTO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112692.1

ORF Insert Sequence:

ATGAAGCGAA CCCCAACCGC CGAGGAACGA GAGCGCGGAG CTAAGAAACT GAGGCTTCTT 
GAAGAGCTGG AAGACACTTG GCTTCCTTAT CTGACCCCCA AAGATGATGA ATTCTATCAG 
CAGTGGCAGC TGAAATACCC TAAGCTAATT CTCCGAGAAG CAGGCAGCGT CCCTGAGGGA 
CTCCACAAAG AGGTTCAAGA AGCCTTCCTC GCACTGCACA AGCATGGCTG CTTATTTCGG 
GACCTGGTCA GGATCCAAGG CAAAGATTTG CTCACGCCAG TATCTCGCCT CCTCATTGGT 
AACCCCGGCT GCACCTACAA GTACCTGAAC ACCAGGCTCT TCACGGTCCC CTGGCCAGTG 
AAGGGCTCTG ATGCAAAGTA CAATGAGGCC GAGATAGGCG CCGCCTGCCA GACCTTCCTC 
AAGCTCAACG ACTACCTGCA GATTGAGACC ATCCAGGCGC TGGAGGAACT CGCTGCCAAG 
GAGAAAGCCA ATATCGACAC CGTGCCGGCG TGTATAGGTC CAGATTTCCC CAGGGTCGGC 
ATGGGGTCAT CCTTTGACGG CCATGACGAG GTGGACAGGA AGAGCAGAGC CGCCTACAAC 
CTAACTTTGT TGAGCTTCAT GGATCCCCAG AAAATGCCGT ACCTGAAAGA GGAGCCCTAC 
TTTGGCATGG GGAAGATGGC TGTGAGCTGG CATCACGATG AAAATCTGGT GGACAGGTCA 
GCGGTGGCAG TGTACAATTA TAGCTGTGAA GGCCCTGAAG AGGAAAGCGA GGATGATCCC 
CAGCTCGAAG GCAGAAATCC CGATGTGTGG CATGTTGGCT TTAAGATCTC ATGGGACATA 
GAGACCCCTG GTTTGGCGAT ACCCCTTCAC CAAGGAGACT GCTACTTTAT GCTGGATGAT 
CTCAATGCCA CCCACCAACA CTGTGTTTTG GCTGGTTTAC CACCCCGGTT TAGTTCCACC 
CACCGAGTGG CCGAGTGCTC GACGGGAGCC TTGGATTACA TCTTACAGCG CTGCCAGTTG 
GCCCTGCAGA ATGTCCGTGA TGAGGCGGAC AGTGGTGAAG TCTCTTTGAA ATCCTTGGAG 
CCTGCGGTTT TGAAACAAGG AGAAGAAATC CACAACGAGG TCGAGTTTGA GTGGCTGAGA 
CAGTTTTGGT TTCAAGGCAA TCGATACAAA AAGTGCACCG ATTGGTGGTG TCAACCCATG 
ACTCAGCTGG AAGAGCTTTG GAAGAAGATG GAAGGTGCGA CCCATGCTGT GCTTCGTGAA 
GTTAGAAGAG AGGGGGCCCC TGTGGAACAG AGCAGTGACA TCCTGACTGC CATCCTAGCC 
GTGCTCACCA CTCGCCAGAA CCTGAGGAGG GAGTGGCATG CCAGGTGCCA GTCCCGAATT 
GCCCGAACTC TGCCTGTGGA CCAGAAGCCA GAATGCCGGC CGTATTGGGA AAAGGATGAT 
CCCTCCATGC CTCTGCCGTT TGATCTCACA GACACTGTGG CTGAACTCAG AGGTCTGCTT 
CTGGAAGCCA AACCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FTO Promotes Adipogenesis through Inhibition of the Wnt/β-catenin Signaling Pathway in Porcine Intramuscular Preadipocytes.
Animal biotechnology28(4)268-274(2017 Oct)
Chen X,Luo Y,Jia G,Liu G,Zhao H,Huang Z

Nucleotide variability and haplotype heterogeneity at the porcine fat mass and obesity-associated (FTO) gene.
Animal genetics44(1)96-100(2013 Feb)
Fontanesi L,Russo V

Association between polymorphism in the FTO gene and growth and carcass traits in pig crosses.
Genetics, selection, evolution : GSE4413(2012 Apr)
Dvořáková V,Bartenschlager H,Stratil A,Horák P,Stupka R,Cítek J,Sprysl M,Hrdlicová A,Geldermann H

Splice variant identification and expression analysis of the fat mass and obesity-associated (FTO) gene in intact and castrated male pigs.
DNA and cell biology29(12)729-33(2010 Dec)
Huang J,Liu G,Liu Y,Yao Y,Wu K,Fang M

Expression studies of the obesity candidate gene FTO in pig.
Animal biotechnology21(1)51-63(2010)
Madsen MB,Birck MM,Fredholm M,Cirera S

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FTO cDNA ORF clone, Sus scrofa(pig)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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