LOC576141 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC576141
Entrez Gene ID	576141
Full Name	uncharacterized LOC576141
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011676341.1	XP_011674643.1	uncharacterized protein LOC576141
XM_030974580.1	XP_030830440.1	uncharacterized protein LOC576141 isoform X3
XM_030974579.1	XP_030830439.1	uncharacterized protein LOC576141 isoform X2
XM_030974581.1	XP_030830441.1	uncharacterized protein LOC576141 isoform X4
XM_030974578.1	XP_030830438.1	uncharacterized protein LOC576141 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC576141 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC576141 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd21280	XM_011676341.1 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSd69451	XM_030974578.1 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSd69452	XM_030974579.1 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSd69453	XM_030974580.1 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSd69454	XM_030974581.1 Latest version!	Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC576141 ( XM_011676341.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011674643.1 Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), mRNA.

Price & Availability↑

CloneID	OSd21280
Clone ID Related Accession (Same CDS sequence)	XM_011676341.1
Accession Version	XM_011676341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC576141
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011991356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGTCTAAAT GCAGAAGCGG GAGACGACAA CAGGTTATTG AGAAACTGGA TGAGAATGAG 
AGTCTGGAAT ATGAGTTGAC GACCTCTGCT GAATCTTTTA CCATCCAGTT CTGTCGAAAG 
AGAGAAAAAG ACTGCCTTGA GATTGGTAAG AAAAAAGGGG TGGAAAGAGA GAAAGGGGAA 
CNACCCAGTC ACACCCAAGA AAGGAAGCGT GGCGGTAGGA AGAAAGGCGA CAAAGACTCA 
AAGTCGAATG AACTTTCCGG CGGCAAAAAG AGCAGAGGAA GAGGGGGTGA AAAGAGAGAA 
AGGGGAAACA CCGAGTCACC CCAAGAAAGG AAGCGTGGTG ATCGTAAAAA AGGCGACAAA 
GACTCAAAGT CGAATGAAAA GAACAGAGGA GGACGACGTG GAGGAAGCAA GAAAGACTCT 
AAGAGCAGAA AGGGCGGACG AAATCGAGGT CGTGGAAGAT GGGCACACCG GAGAGGCAAA 
TACTATCGAT GGCGTACCGT CAAGGGTGCA GTGAAGAGGA CACGCGCTGT GAGCTTTGAC 
GAACGTCGGA GCGCCATGTC TGTTTTTATC AAGACCAAAG CAACTCGATT CGTCTTAGAA 
TTCTGCCTGT CGTCAGGAGG CAAAGACTGC TATACTGCTT CCCTGAGTTC ACAGCGTGAT 
TCGGCCTCCT TCTTCGAGTA CGGCGGCCAA AGAACGGGCG ATAGTCAAGA TGGAGCATTC 
ATTCGATTTA AACGAAGAAC CAGATTATAT GTATCAGTCT CACGTACTAA ACTGACGATC 
TACAATGACA AGGGAGACGA TCTCTCAGTC GCTTTACCCG AGTCATCAGT CATCCAAGAT 
GTCTACATGT CCACATCAGA TGGGGACTGG ACGAAGGTTA GATTCCGCCT ACGAAGATCT 
GGTAGAACCC TTTACAGCTC CACTGGTCCT CTTGCTCGCG GTGCTGCCTT CGAAGATATT 
GGTTCTAAAG CAACGTCATT CTCCTGTCTA ATACGCACCG TGGCCAGCGC CGTCACTTTC 
GAAATTTGCT TCGATTCTCA GGGAAATGAT TGTTACACAA TCGAACTGAG CACGCAACCC 
AAACAGACAT CCCGAATCCT GTATGGAGGA AAATTAGTGA GCAACGAAGA AATTGGTATT 
TTTATTACCC CTAGCAAGGA GTCGAAAATC TTCTTTCGCA TCGAGAAACG GCAGCTGGAG 
ATCTGGAATA AGGAACCAAA CAATGAACGC CAACTCATCG CTAAAATCCG CGGAAAAGGT 
CGCAGTTTTA ATTCCATTTG GGCCTCTACT AGTGATGGTG AATTCACCGA GCTTCAGATG 
GTCGAGACTG AAGCCGTTAG AGAGTTTTCA TCCACGACTA CTCTTGAGAG AACATCGATT 
GCCACTCAAC TTCAGTTCCC AGGGCAACTG GTGTTAAGCT TTGCTCTCGA GGCTGACAAG 
GTCTTCATGG AGTTTCGTCG CCCAGATGGG AAGAGTTATA CGATAGAAAT GGGTACGAAA 
GCAGGTCAGC CATCTGTTAT TCGCAGTGGG GGTAGTAACC TAGTAGATCC ACAGGACCAA 
CCATTCCTCG AGTTTGGCAA TGACATTGAC CCACCTAACC AACTCGTCAT CGATGTGTTC 
GAGTCTAGCG TGACTGTTTC TACTGCCCGT GGTCAAAAGA TGGTGGCACC TGTTGCAGAA 
GCTTATCTCT ACAACCATTT CGACATTTGG ACGGTGGGTG GTGGTATTGC TCTCGCTGTG 
GAAGAAATCG AAGATGAAGA TTCGAAAGAT TCTCTTGCAA GTAAGACAGA GATTGAAGAA 
ACAGAACAGG AAATAGAATC AGTAGAAGAG GAACTTAAGG GTAACAACCC CGGCGTTCAA 
CCAGTGCGAA AACGAAAAGG AAAAACAAGC GGCAAAAGAC GAGGAAAAAC AGGCGGCAGA 
AGACGCGGAA AAGCTAAAGA GTCAAAATCA GATGACGACA CAGAACCAGA AAACGAACCA 
GTAGAAGAGG CCAAGGGTGA CAGCCCCAGC GTTCAACAAG AGCGAAAACG AAATGGAAAA 
AAAGGCGGCA AAAGACGAGG AAAAACAGGC GACAGAAGAC GCGGAAAAGC TAAAGAGTCA 
AAATCAGATG ACGACACAGA ACCAGAAAAC GAACCAGTAG AAGAGGCCAA GGGTGACAGC 
CCCAGCGTTC AACAAGAGCG AAAACGAAAT GGAAAAAAAG GCGGCAAAAG ACGAGGAAAA 
ACAGACGACA GAAGACGCGG AAAAGCTAAA GAGTCATCAG ATGACGACAC AGAACCAGAA 
AACGAACCAG TAGAAGAGGC CAAGGGTGAC AGCCCCGGCA TTCAACAAGA GCGAAAACGA 
AAAGGAAAAA TAGGCGGCAA GAGACGAGGA AAAACAGGCG GCAGAAGACG CGGAAAAGCT 
AAAGAGTCAC AATCAGATGA CGACACAGAA CCAGAAAACG AACCAGTAGA AGAGGCCAAG 
GGTGACAGCC CCAGCGTTCA ACAAGAGCGA AAACGAAATG GAAAAAAAGG CGGCAAAAGA 
CGAGGAAAAA CAGGCGACAG AAGACGCGGA AAAGCTAAAG AGTCAAAATC AGATGACGAC 
ACAGAACCAG AAAACGAACC AGTAGAAGAG GCCAAGGGTG ACAGCCCCAG CGTTCAACAA 
GAGCGAAAAC GAAATGGAAA AAAAGGCGGC AAAAGACGAG GAAAAACAGG CGACAGAAGA 
CGCGGAAAAG CTAAAGAGTC ATCAGATGAC GACACAGAAC CAGAAAACGA ACCAGTAGAA 
GAGGCCAAGG GTGACAGCCC CGGCATTCAA CAAGAGCGAA AACGAAAAGG AAAAATAGGC 
GGCAAGAGAC GAGGAAAAAC AGGCGGCAGA ATACGCGGAA AAGCTAAAGA GTCACAATCA 
GATGACGACA CAGAACCAGA AAACGAACCA GTAGAAGAGG CCAAGGGTGA CAGCCCCAGC 
GTTCAACAAG AGCGAAAACG AAATGGAAAA AAAGGCGGCA AAAGACGAGG AAAAACAGGC 
GACAGAAGAC GCGGAAAAGC TAAAGAGTCA TCAGATGACG ACACAGAACC TGTTATTGAA 
CCAGTAATAC AGGACACATT AGAAGAAACG AAACCTGTGA ACAGTGAAGT ATTCGAAGAA 
TTGAAGGGTA ACAACCCTGG CGTTGAAGTA AAGAGAACTA GAGGTGACAG AATCATAGAC 
AATCAAGATA TTGAAGACAC AACACAAGAG TCATCTCTGA CACCAACTCA AGTTCCACCA 
ACAATTGAGA CAATACGAAA CACGGGATCA ACCGATGAGC CAGAGGTCAA AGAGAAAGTT 
AGCCAGGACG TAGTAGAGCC GGTCGTGAAA CCGTTCAACG AAGAACCTGA TAAGTCAGCC 
ACTCTTGAGA CCAACGTACT TGGCGTCGCT GCGTTCTTCG ATCAAACCTT GGATATAGAT 
CGTTTATTAC AGCTTCAGTT TGATTTCAGT GTCACCATTA ACACCGGTTT CGCTATCCTC 
TTGACACAAG GACAAATTCT TAGGGGAGAT TATTACTCAA TCGGTATAAC CGAGGGAAAC 
GTCTACATGG TCCAGCGATG TAATAAGGAA ACAGCGGAAT GTAATCGACT CTTCAGCAGG 
GAAATTGATC CACTTGGTGA AGGAACAGGA ATATTCACGC TGAAGATTCA GAAGATCCTA 
AATGGCAATG TAGACCCTGA TGCTGATGGA GCATACTCCT ACTGGTTTTC ATTGCTGTAT 
GAAGGAGCTG TGGTCGGAGA ACAGGTGGAA GTACAGTATC CAAATACATT CTATATTAGG 
TACATTGGAT GCATCTCCTA CAAGTACGTG GCCACGTGGA ATCTCTTCTC TGTCGTCTCA 
CCGGGTTCTA CCCAGAGATC CTCTGCAATC ATACTCCGGA ACGCCGAAGA AACAAGTAGT 
CTGGAGGTGA ATTCATTTGA TCGCGAAGTT GTATTCAGTG TCAAAGCCCA AGATTTTTTG 
ATAATTCGTC TTTACTCCGT TGGCGACGTC GTCTACACAA TCCTCGTTGG AGACGAAGGC 
AATAACTATG TTACGGGATC GTCCGGTACT GGTGATAAAT CTCCGGATGA CGCGTCGCGT 
CGCGTTGAAG GAGGTGTGTT AAGCCTGGCG GGCAAGCAGT ACTACCTTTC TTGGCGAAAC 
GGCAGGATGA AGTTCGGCGA AACGGAGGAG GACAGCGAGG CGATGATGAA TTGGGAGATT 
GGGGAAATCT CGGTGACTAA GCTAGAATTC ATTGCTGCTT ACGACAGCTT GTATCTCATA 
CACACATACA AGTACTTCTA CGTATTTGGC GATGTTTTGC AGGCGACTCA TGATCGAATA 
CGGGCAACTT CTCTCACTTT CTTCACTCTC CGTCGCGAGC GTGGCGTGCC ATACATCCAC 
CTCAAGATCA AGGCCTCTGG GACTGTCTTG ATTGCGCTCG CCAAAGACCA GGAGCTTTCT 
GAATCCGACA TGGTTTTGAT CAAGATGAGT TCCCGGAGGA TAACTCTCTC CACATGCCTT 
GGTTGCGAAA CAATCTACTC GGTTCCGATC CCTGAAGACC AAGCAGCCCT TCTAGATCCG 
TCCTCATTCC TTGAGTACCA GATCACGTGT GAAGGCAACA GTCTGAAGAT CTTCTTAACC 
GGTCAGAAGG AACCCTTGCT GGACTATGGT GGCATTGATC GAGACGCCCA CTATGGCTAT 
GATGGCTTCG GAGGAACTAT CGGTAGCAAT TATCACATCA AGCTCAACCA AGGAACGATC 
TACAGAGAGG AGCATTGTCT TGACGTCAAC ACCTTCTTGG GTGGAGGAAC GAAGCTTCTG 
AAGATCGTCC ACTCTGACCA GATGATTGCT TTATTTGATG CTGAGAAACA GCTCGAGGCA 
GAGCTTGCCA TGGAAGATAC GAAGACGGAG CTGCGTACTA CCCGTGGTGG CGAGCCCATA 
AAGATAAGCC AAAGTAGTAG TTGCATCTTC CACGCTCCCT ACACTCTAGT CTGGATGGAC 
TTCAGCGGTG GGGCTATTCG GTATGGTACC TACGACGAGG CGAATCGCCT GAGAAGTGAC 
CCGTGTATCA CCTACGACCT CGGTGATGAT CAGGAGGTCA AATACATGTC GTTTGGTCAA 
CAGCATTCCT CGTGGGATGT TACACATCAC GTGATTGAGA AGAGGATTGT GTACAAGGAG 
TTGGTCTCCC ATGTACAAGA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011674643.1
CDS	1..5187
Translation	MSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIGKKKGVEREKGEnullPSHTQERKRGGRKKGDKDSKSNELSGGKKSRGRGGEKRERGNTESPQERKRGDRKKGDKDSKSNEKNRGGRRGGSKKDSKSRKGGRNRGRGRWAHRRGKYYRWRTVKGAVKRTRAVSFDERRSAMSVFIKTKATRFVLEFCLSSGGKDCYTASLSSQRDSASFFEYGGQRTGDSQDGAFIRFKRRTRLYVSVSRTKLTIYNDKGDDLSVALPESSVIQDVYMSTSDGDWTKVRFRLRRSGRTLYSSTGPLARGAAFEDIGSKATSFSCLIRTVASAVTFEICFDSQGNDCYTIELSTQPKQTSRILYGGKLVSNEEIGIFITPSKESKIFFRIEKRQLEIWNKEPNNERQLIAKIRGKGRSFNSIWASTSDGEFTELQMVETEAVREFSSTTTLERTSIATQLQFPGQLVLSFALEADKVFMEFRRPDGKSYTIEMGTKAGQPSVIRSGGSNLVDPQDQPFLEFGNDIDPPNQLVIDVFESSVTVSTARGQKMVAPVAEAYLYNHFDIWTVGGGIALAVEEIEDEDSKDSLASKTEIEETEQEIESVEEELKGNNPGVQPVRKRKGKTSGKRRGKTGGRRRGKAKESKSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESKSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTDDRRRGKAKESSDDDTEPENEPVEEAKGDSPGIQQERKRKGKIGGKRRGKTGGRRRGKAKESQSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESKSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESSDDDTEPENEPVEEAKGDSPGIQQERKRKGKIGGKRRGKTGGRIRGKAKESQSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESSDDDTEPVIEPVIQDTLEETKPVNSEVFEELKGNNPGVEVKRTRGDRIIDNQDIEDTTQESSLTPTQVPPTIETIRNTGSTDEPEVKEKVSQDVVEPVVKPFNEEPDKSATLETNVLGVAAFFDQTLDIDRLLQLQFDFSVTINTGFAILLTQGQILRGDYYSIGITEGNVYMVQRCNKETAECNRLFSREIDPLGEGTGIFTLKIQKILNGNVDPDADGAYSYWFSLLYEGAVVGEQVEVQYPNTFYIRYIGCISYKYVATWNLFSVVSPGSTQRSSAIILRNAEETSSLEVNSFDREVVFSVKAQDFLIIRLYSVGDVVYTILVGDEGNNYVTGSSGTGDKSPDDASRRVEGGVLSLAGKQYYLSWRNGRMKFGETEEDSEAMMNWEIGEISVTKLEFIAAYDSLYLIHTYKYFYVFGDVLQATHDRIRATSLTFFTLRRERGVPYIHLKIKASGTVLIALAKDQELSESDMVLIKMSSRRITLSTCLGCETIYSVPIPEDQAALLDPSSFLEYQITCEGNSLKIFLTGQKEPLLDYGGIDRDAHYGYDGFGGTIGSNYHIKLNQGTIYREEHCLDVNTFLGGGTKLLKIVHSDQMIALFDAEKQLEAELAMEDTKTELRTTRGGEPIKISQSSSCIFHAPYTLVWMDFSGGAIRYGTYDEANRLRSDPCITYDLGDDQEVKYMSFGQQHSSWDVTHHVIEKRIVYKELVSHVQEP

Target ORF information:

RefSeq Version	XM_011676341.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011676341.1

ORF Insert Sequence:

ATGTCTAAAT GCAGAAGCGG GAGACGACAA CAGGTTATTG AGAAACTGGA TGAGAATGAG 
AGTCTGGAAT ATGAGTTGAC GACCTCTGCT GAATCTTTTA CCATCCAGTT CTGTCGAAAG 
AGAGAAAAAG ACTGCCTTGA GATTGGTAAG AAAAAAGGGG TGGAAAGAGA GAAAGGGGAA 
CNACCCAGTC ACACCCAAGA AAGGAAGCGT GGCGGTAGGA AGAAAGGCGA CAAAGACTCA 
AAGTCGAATG AACTTTCCGG CGGCAAAAAG AGCAGAGGAA GAGGGGGTGA AAAGAGAGAA 
AGGGGAAACA CCGAGTCACC CCAAGAAAGG AAGCGTGGTG ATCGTAAAAA AGGCGACAAA 
GACTCAAAGT CGAATGAAAA GAACAGAGGA GGACGACGTG GAGGAAGCAA GAAAGACTCT 
AAGAGCAGAA AGGGCGGACG AAATCGAGGT CGTGGAAGAT GGGCACACCG GAGAGGCAAA 
TACTATCGAT GGCGTACCGT CAAGGGTGCA GTGAAGAGGA CACGCGCTGT GAGCTTTGAC 
GAACGTCGGA GCGCCATGTC TGTTTTTATC AAGACCAAAG CAACTCGATT CGTCTTAGAA 
TTCTGCCTGT CGTCAGGAGG CAAAGACTGC TATACTGCTT CCCTGAGTTC ACAGCGTGAT 
TCGGCCTCCT TCTTCGAGTA CGGCGGCCAA AGAACGGGCG ATAGTCAAGA TGGAGCATTC 
ATTCGATTTA AACGAAGAAC CAGATTATAT GTATCAGTCT CACGTACTAA ACTGACGATC 
TACAATGACA AGGGAGACGA TCTCTCAGTC GCTTTACCCG AGTCATCAGT CATCCAAGAT 
GTCTACATGT CCACATCAGA TGGGGACTGG ACGAAGGTTA GATTCCGCCT ACGAAGATCT 
GGTAGAACCC TTTACAGCTC CACTGGTCCT CTTGCTCGCG GTGCTGCCTT CGAAGATATT 
GGTTCTAAAG CAACGTCATT CTCCTGTCTA ATACGCACCG TGGCCAGCGC CGTCACTTTC 
GAAATTTGCT TCGATTCTCA GGGAAATGAT TGTTACACAA TCGAACTGAG CACGCAACCC 
AAACAGACAT CCCGAATCCT GTATGGAGGA AAATTAGTGA GCAACGAAGA AATTGGTATT 
TTTATTACCC CTAGCAAGGA GTCGAAAATC TTCTTTCGCA TCGAGAAACG GCAGCTGGAG 
ATCTGGAATA AGGAACCAAA CAATGAACGC CAACTCATCG CTAAAATCCG CGGAAAAGGT 
CGCAGTTTTA ATTCCATTTG GGCCTCTACT AGTGATGGTG AATTCACCGA GCTTCAGATG 
GTCGAGACTG AAGCCGTTAG AGAGTTTTCA TCCACGACTA CTCTTGAGAG AACATCGATT 
GCCACTCAAC TTCAGTTCCC AGGGCAACTG GTGTTAAGCT TTGCTCTCGA GGCTGACAAG 
GTCTTCATGG AGTTTCGTCG CCCAGATGGG AAGAGTTATA CGATAGAAAT GGGTACGAAA 
GCAGGTCAGC CATCTGTTAT TCGCAGTGGG GGTAGTAACC TAGTAGATCC ACAGGACCAA 
CCATTCCTCG AGTTTGGCAA TGACATTGAC CCACCTAACC AACTCGTCAT CGATGTGTTC 
GAGTCTAGCG TGACTGTTTC TACTGCCCGT GGTCAAAAGA TGGTGGCACC TGTTGCAGAA 
GCTTATCTCT ACAACCATTT CGACATTTGG ACGGTGGGTG GTGGTATTGC TCTCGCTGTG 
GAAGAAATCG AAGATGAAGA TTCGAAAGAT TCTCTTGCAA GTAAGACAGA GATTGAAGAA 
ACAGAACAGG AAATAGAATC AGTAGAAGAG GAACTTAAGG GTAACAACCC CGGCGTTCAA 
CCAGTGCGAA AACGAAAAGG AAAAACAAGC GGCAAAAGAC GAGGAAAAAC AGGCGGCAGA 
AGACGCGGAA AAGCTAAAGA GTCAAAATCA GATGACGACA CAGAACCAGA AAACGAACCA 
GTAGAAGAGG CCAAGGGTGA CAGCCCCAGC GTTCAACAAG AGCGAAAACG AAATGGAAAA 
AAAGGCGGCA AAAGACGAGG AAAAACAGGC GACAGAAGAC GCGGAAAAGC TAAAGAGTCA 
AAATCAGATG ACGACACAGA ACCAGAAAAC GAACCAGTAG AAGAGGCCAA GGGTGACAGC 
CCCAGCGTTC AACAAGAGCG AAAACGAAAT GGAAAAAAAG GCGGCAAAAG ACGAGGAAAA 
ACAGACGACA GAAGACGCGG AAAAGCTAAA GAGTCATCAG ATGACGACAC AGAACCAGAA 
AACGAACCAG TAGAAGAGGC CAAGGGTGAC AGCCCCGGCA TTCAACAAGA GCGAAAACGA 
AAAGGAAAAA TAGGCGGCAA GAGACGAGGA AAAACAGGCG GCAGAAGACG CGGAAAAGCT 
AAAGAGTCAC AATCAGATGA CGACACAGAA CCAGAAAACG AACCAGTAGA AGAGGCCAAG 
GGTGACAGCC CCAGCGTTCA ACAAGAGCGA AAACGAAATG GAAAAAAAGG CGGCAAAAGA 
CGAGGAAAAA CAGGCGACAG AAGACGCGGA AAAGCTAAAG AGTCAAAATC AGATGACGAC 
ACAGAACCAG AAAACGAACC AGTAGAAGAG GCCAAGGGTG ACAGCCCCAG CGTTCAACAA 
GAGCGAAAAC GAAATGGAAA AAAAGGCGGC AAAAGACGAG GAAAAACAGG CGACAGAAGA 
CGCGGAAAAG CTAAAGAGTC ATCAGATGAC GACACAGAAC CAGAAAACGA ACCAGTAGAA 
GAGGCCAAGG GTGACAGCCC CGGCATTCAA CAAGAGCGAA AACGAAAAGG AAAAATAGGC 
GGCAAGAGAC GAGGAAAAAC AGGCGGCAGA ATACGCGGAA AAGCTAAAGA GTCACAATCA 
GATGACGACA CAGAACCAGA AAACGAACCA GTAGAAGAGG CCAAGGGTGA CAGCCCCAGC 
GTTCAACAAG AGCGAAAACG AAATGGAAAA AAAGGCGGCA AAAGACGAGG AAAAACAGGC 
GACAGAAGAC GCGGAAAAGC TAAAGAGTCA TCAGATGACG ACACAGAACC TGTTATTGAA 
CCAGTAATAC AGGACACATT AGAAGAAACG AAACCTGTGA ACAGTGAAGT ATTCGAAGAA 
TTGAAGGGTA ACAACCCTGG CGTTGAAGTA AAGAGAACTA GAGGTGACAG AATCATAGAC 
AATCAAGATA TTGAAGACAC AACACAAGAG TCATCTCTGA CACCAACTCA AGTTCCACCA 
ACAATTGAGA CAATACGAAA CACGGGATCA ACCGATGAGC CAGAGGTCAA AGAGAAAGTT 
AGCCAGGACG TAGTAGAGCC GGTCGTGAAA CCGTTCAACG AAGAACCTGA TAAGTCAGCC 
ACTCTTGAGA CCAACGTACT TGGCGTCGCT GCGTTCTTCG ATCAAACCTT GGATATAGAT 
CGTTTATTAC AGCTTCAGTT TGATTTCAGT GTCACCATTA ACACCGGTTT CGCTATCCTC 
TTGACACAAG GACAAATTCT TAGGGGAGAT TATTACTCAA TCGGTATAAC CGAGGGAAAC 
GTCTACATGG TCCAGCGATG TAATAAGGAA ACAGCGGAAT GTAATCGACT CTTCAGCAGG 
GAAATTGATC CACTTGGTGA AGGAACAGGA ATATTCACGC TGAAGATTCA GAAGATCCTA 
AATGGCAATG TAGACCCTGA TGCTGATGGA GCATACTCCT ACTGGTTTTC ATTGCTGTAT 
GAAGGAGCTG TGGTCGGAGA ACAGGTGGAA GTACAGTATC CAAATACATT CTATATTAGG 
TACATTGGAT GCATCTCCTA CAAGTACGTG GCCACGTGGA ATCTCTTCTC TGTCGTCTCA 
CCGGGTTCTA CCCAGAGATC CTCTGCAATC ATACTCCGGA ACGCCGAAGA AACAAGTAGT 
CTGGAGGTGA ATTCATTTGA TCGCGAAGTT GTATTCAGTG TCAAAGCCCA AGATTTTTTG 
ATAATTCGTC TTTACTCCGT TGGCGACGTC GTCTACACAA TCCTCGTTGG AGACGAAGGC 
AATAACTATG TTACGGGATC GTCCGGTACT GGTGATAAAT CTCCGGATGA CGCGTCGCGT 
CGCGTTGAAG GAGGTGTGTT AAGCCTGGCG GGCAAGCAGT ACTACCTTTC TTGGCGAAAC 
GGCAGGATGA AGTTCGGCGA AACGGAGGAG GACAGCGAGG CGATGATGAA TTGGGAGATT 
GGGGAAATCT CGGTGACTAA GCTAGAATTC ATTGCTGCTT ACGACAGCTT GTATCTCATA 
CACACATACA AGTACTTCTA CGTATTTGGC GATGTTTTGC AGGCGACTCA TGATCGAATA 
CGGGCAACTT CTCTCACTTT CTTCACTCTC CGTCGCGAGC GTGGCGTGCC ATACATCCAC 
CTCAAGATCA AGGCCTCTGG GACTGTCTTG ATTGCGCTCG CCAAAGACCA GGAGCTTTCT 
GAATCCGACA TGGTTTTGAT CAAGATGAGT TCCCGGAGGA TAACTCTCTC CACATGCCTT 
GGTTGCGAAA CAATCTACTC GGTTCCGATC CCTGAAGACC AAGCAGCCCT TCTAGATCCG 
TCCTCATTCC TTGAGTACCA GATCACGTGT GAAGGCAACA GTCTGAAGAT CTTCTTAACC 
GGTCAGAAGG AACCCTTGCT GGACTATGGT GGCATTGATC GAGACGCCCA CTATGGCTAT 
GATGGCTTCG GAGGAACTAT CGGTAGCAAT TATCACATCA AGCTCAACCA AGGAACGATC 
TACAGAGAGG AGCATTGTCT TGACGTCAAC ACCTTCTTGG GTGGAGGAAC GAAGCTTCTG 
AAGATCGTCC ACTCTGACCA GATGATTGCT TTATTTGATG CTGAGAAACA GCTCGAGGCA 
GAGCTTGCCA TGGAAGATAC GAAGACGGAG CTGCGTACTA CCCGTGGTGG CGAGCCCATA 
AAGATAAGCC AAAGTAGTAG TTGCATCTTC CACGCTCCCT ACACTCTAGT CTGGATGGAC 
TTCAGCGGTG GGGCTATTCG GTATGGTACC TACGACGAGG CGAATCGCCT GAGAAGTGAC 
CCGTGTATCA CCTACGACCT CGGTGATGAT CAGGAGGTCA AATACATGTC GTTTGGTCAA 
CAGCATTCCT CGTGGGATGT TACACATCAC GTGATTGAGA AGAGGATTGT GTACAAGGAG 
TTGGTCTCCC ATGTACAAGA GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC576141 ( XM_030974578.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030830438.1 Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X1, mRNA.

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CloneID	OSd69451
Clone ID Related Accession (Same CDS sequence)	XM_030974578.1
Accession Version	XM_030974578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC576141 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAA TGAACGCCCT GGCGTTTGGC GCTGTTCTGC TGTTAGCTGC AGTCGCGTCG 
ACCTCAGGCG GCGGTAGCAC CAGTCAAGTT GCTGATAATA AAGAGCGATT CTCATACAGC 
GGCGGAGGTG GTCTCACAGG CCTGAACGTC AAGCAAGGTG GTTTTACTCA GGTTTCCCAC 
TCGACAAAAG AAAAGACTGT CACCACGGAG AAAGAAACTA GGAAGAGCAA ATCAAAAAAG 
CGGAAAGGCG GTAAGGTGTC TACTCAAGAG ACTTCAAAGA AAGAAACTAA CGGGGGCAAA 
GTGAAGAACC AGAGAGGCAA TACGCAAGAG ACTTCACGAA AACGAAAAGG AAGGGGAAAG 
AATAAAGGAA ACGAAAAAAC TGAAGTCAAA CAACGAGAAG GAAATCGCGG TCGAGATGGA 
GATGGCAAAG GAGATCGTAA AGGAAGAAAG GGAAGTACTG AGACGGTGAT CAATAAAGAA 
AGGAGCGGTG GTCGGCGTCG AAGTAAAAAC AAGAGTGGTA AGGGATCGAC CAAAGGATCG 
AGCAGTAAAA AGAGTGGAAG AAGAGATTGG AAGATGTCTA AATGCAGAAG CGGGAGACGA 
CAACAGGTTA TTGAGAAACT GGATGAGAAT GAGAGTCTGG AATATGAGTT GACGACCTCT 
GCTGAATCTT TTACCATCCA GTTCTGTCGA AAGAGAGAAA AAGACTGCCT TGAGATTGTT 
CTGAGCTCAT CTGGTGAATC ATCCATTAAG CTTGATGGAC AGGATTTGGG CGATCCCATT 
TCGGACTTCT TTTACAAGAA AGGGGAGAAG TTCTCCTTCA CTATCACCTA TACATCCCAG 
AGTGTGGTTG TGAGGAACAG CAAATCAGGA TTAGAACTTG TTGCATCGGT CGATGGTGAT 
GAACTTAAGG GTTTCAGGTA CTGCTATGGC ACTGCAAACG GAGGAGAAGG AGATTCTACA 
TTCCAGATAA GGGAACGTGA ATTTTCCGAA TCAGAGATCT CATCATTTGA AAAGCGGTAT 
AGGGGCAAGG GAGGTAGAAA GAGGGGTGGA AAGAGAGAAA GGGGAAACGC CCAGTCACCT 
CAAGAAAGGA AGCGTGGCGG TAGGAAGAAA GGCGACAAAG ACTCAAAGTC GAATGAACTT 
TCCGGCGGCA AAAAGAGCAG AGGAAGAGAT AAAGGATCGA AAAAAGACAG GAGCAGTAAA 
AAGAGTGGAA GAAGAGATTG GAAGATGTCT AAATGCAGAA GCGGGAGACG ACAACAGGTT 
ATTGAGAAAC TGGATGAGAA TGAGAGTCTG GAATATGAGT TGACGACCTC TGCTGAATCT 
TTTACCATCC AGTTCTGTCG AAAGAGAGAA AAAGACTGCC TTGAGATTGT TCTGAGCTCA 
TCTGGTGAAT CATCCATTAA GCTTGATGGA CAGGATTTGG GCGATCCCAT TTCGGACTTC 
TTTTACAAGA AAGGGGAGAA GTTCTCCTTC ACTATCACCT ATACATCCCA GCGTGTGGTT 
GTGAGGAACA GCAAATCAGG ATTAGAACTT GTTGCATCGG TCGATGGTGA TGAACTTAAG 
GGTTTTAGGT ACTGCTATGG CACTGCAAAC GGAGGAGAAG GAGATTCTAC ATTCCAGATA 
AGGGAACGTG AATTTTCCGA ATCAGAGATC TCATCATTTG AAAAGCGGTA TAGGGGCAAG 
GGAGGTAGAA AGAGGGGTGG AAAGAGAGAA AGGGGAAACG CCCAGTCACC TCAAGAAAGG 
AAGCGTGGCG GTAGGAAGAA AGGCGACAAA GACTCAAAGT CGAATGAACT TTCCGGCGGC 
AAAAAGAGCA GAGGAAGAGA TAAAGGATCG AAAAAAGACA GGAGCAGTAA AAAGAGTGGA 
AGAAGAGATT GGAAGATGTC TAAATGCAGA AGCGGGAGAC GACAACAGGT TATTGAGAAA 
CTGGATGAGA ATGAGAGTCT GGAATATGAG TTGACGACCT CTGCTGAATC TTTTACCATC 
CAGTTCTGTC GAAAGAGAGA AAAAGACTGC CTTGAGATTG TTCTGAGCTC ATCTGGTGAA 
TCATCCATTA AGCTTGATGG ACAGGATTTG GGCGATCCCA TTTCGGACTT CTTTTACAAG 
AAAGGGGAGA AGTTCTCCTT CACTATCACC TATACATCCC AGCGTGTGGT TGTGAGGAAC 
AGCAAATCAG GATTAGAACT TGTTGCATCG GTCGATGGTG ATGAACTTAA GGGTTTTAGG 
TACTGCTATG GCACTGCAAA CGGAGGAGAA GGAGATTCTA CATTCCAGAT AAGGGAACGT 
GAATTTTCCG AATCAGAGAT CTCATCATTT GAAAAGCGGT ATAGGGGCAA GGGAGGTAGA 
AAGAGGGGTG GAAAGAGAGA AAGGGGAAAC ACCCAGTCAC CCCAAGAAAG GAAGCGTGGC 
GGTAGGAAGA AAGGCGACAA AGACTCAAAG TCGAATGAAC TTTCCGGCGG CAAAAAGAGC 
AGAGGAAGAG ATAAAGGATC GAAAAAAGAC AGGAGCAGTA AAAAGAGTGG AAGAAGAGAT 
TGGAAGATGT CTAAATGCAG AAGCGGGAGA CGACAACAGG TTATTGAGAA ACTGGATGAG 
AATGAGAGTC TGGAATATGA GTTGACGACC TCTGCTGAAT CTTTTACCAT CCAGTTCTGT 
CGAAAGAGAG AAAAAGACTG CCTTGAGATT GTTCTGAGCT CATCTGGTGA ATCATCCATT 
AAGCTTGATG GACAGGATTT GGGCGATCCC ATTTCGGACT TCTTTTACAA GAAAGGGGAG 
AAGTTCTCCT TCACTATCAC CTATACATCC CAGCGTGTGG TTGTGAGGAA CAGCAAATCA 
GGATTAGAAC TTGTTGCATC GGTCGATGGT GATGAACTTA AGGGTTTCAG GTACTGCTAT 
GGCACTGCAA ACGGAGGAGA AGGAGATTCT ACATTCCAGA TAAGGGAACG TGAATTTTCC 
GAATCAGAGA TCTCATCATT TGAAAAGCGG TATAGGGGCA AGGGAGGTAG AAAGAGGGGT 
GGAAAGAGAG AAAGGGGAAA CACCCAGTCA CCCCAAGAAA GGAAGCGTGG CGGTAGGAAG 
AAAGGCGACA AAGACTCAAA GTCGAATGAA CTTTCCGGCG GCAAAAAGAG CAGAGGAAGA 
GGGGGTGAAA AGAGAGAAAG GGGAAACACC GAGTCACCCC AAGAAAGGAA GCGTGGTGAT 
CGTAAAAAAG GCGACAAAGA CTCAAAGTCG AATGAAAAGA ACAGAGGAGG ACGACGTGGA 
GGAAGCAAGA AAGACTCTAA GAGCAGAAAG GGCGGACGAA ATCGAGGTCG TGGAAGATGG 
GCACACCGGA GAGGCAAATA CTATCGATGG CGTACCGTCA AGGGTGCAGT GAAGAGGACA 
CGCGCTGTGA GCTTTGACGA ACGTCGGAGC GCCATGTCTG TTTTTATCAA GACCAAAGCA 
ACTCGATTCG TCTTAGAATT CTGCCTGTCG TCAGGAGGCA AAGACTGCTA TACTGCTTCC 
CTGAGTTCAC AGCGTGATTC GGCCTCCTTC TTCGAGTACG GCGGCCAAAG AACGGGCGAT 
AGTCAAGATG GAGCATTCAT TCGATTTAAA CGAAGAACCA GATTATATGT ATCAGTCTCA 
CGTACTAAAC TGACGATCTA CAATGACAAG GGAGACGATC TCTCAGTCGC TTTACCCGAG 
TCATCAGTCA TCCAAGATGT CTACATGTCC ACATCAGATG GGGACTGGAC GAAGGTTAGA 
TTCCGCCTAC GAAGATCTGG TAGAACCCTT TACAGCTCCA CTGGTCCTCT TGCTCGCGGT 
GCTGCCTTCG AAGATATTGG TTCTAAAGCA ACGTCATTCT CCTGTCTAAT ACGCACCGTG 
GCCAGCGCCG TCACTTTCGA AATTTGCTTC GATTCTCAGG GAAATGATTG TTACACAATC 
GAACTGAGCA CGCAACCCAA ACAGACATCC CGAATCCTGT ATGGAGGAAA ATTAGTGAGC 
AACGAAGAAA TTGGTATTTT TATTACCCCT AGCAAGGAGT CGAAAATCTT CTTTCGCATC 
GAGAAACGGC AGCTGGAGAT CTGGAATAAG GAACCAAACA ATGAACGCCA ACTCATCGCT 
AAAATCCGCG GAAAAGGTCG CAGTTTTAAT TCCATTTGGG CCTCTACTAG TGATGGTGAA 
TTCACCGAGC TTCAGATGGT CGAGACTGAA GCCGTTAGAG AGTTTTCATC CACGACTACT 
CTTGAGAGAA CATCGATTGC CACTCAACTT CAATTCCCAG GGCAACTGGT GTTAAGCTTT 
GCTCTCGAGG CTGACAAGGT CTTCATGGAG TTTCGTCGCC CAGATGGGAA GAGTTATACG 
ATAGAAATGG GTACGAAAGC AGGTCAGCCA TCTGTTATTC GCAGTGGGGG TAGTAACCTA 
GTAGATCCAC AGGACCAACC ATTCCTCGAG TTTGGCAATG ACATTGACCC ACCTAACCAA 
CTCGTCATCG ATGTGTTCGA GTCTAGCGTG ACTGTTTCTA CTGCCCGTGG TCAAAAGATG 
GTGGCACCTG TTGCAGAAGC TTATCTCTAC AACCATTTCG ACATTTGGAC GGTGGGTGGT 
GGTATTGCTC TCGCTGTGGA AGAAATCGAA GATGAAGATT CGAAAGATTC TCTTGCAAGT 
AAGACAGAGA TTGAAGAAAC AGAACAGGAA ATAGAATCAG TAGAAGAGGA ACTTAAGGGT 
AACAACCCCG GCGTTCAACC AGTGCGAAAA CGAAAAGGAA AAACAAGCGG CAAAAGACGA 
GGAAAAACAG GCGGCAGAAG ACGCGGAAAA GCTAAAGAGT CAAAATCAGA TGACGACACA 
GAACCAGAAA ACGAACCAGT AGAAGAGGCC AAGGGTGACA GCCCCAGCGT TCAACAAGAG 
CGAAAACGAA ATGGAAAAAA AGGCGGCAAA AGACGAGGAA AAACAGGCGA CAGAAGACGC 
GGAAAAGCTA AAGAGTCAAA ATCAGATGAC GACACAGAAC CAGAAAACGA ACCAGTAGAA 
GAGGCCAAGG GTGACAGCCC CAGCGTTCAA CAAGAGCGAA AACGAAATGG AAAAAAAGGC 
GGCAAAAGAC GAGGAAAAAC AGGCGACAGA AGACGCGGAA AAGCTAAAGA GTCATCAGAT 
GACGACACAG AACCAGAAAA CGAACCAGTA GAAGAGGCCA AGGGTGACAG CCCCGGCATT 
CAACAAGAGC GAAAACGAAA AGGAAAAATA GGCGGCAAGA GACGAGGAAA AACAGGCGGC 
AGAATACGCG GAAAAGCTAA AGAGTCACAA TCAGATGACG ACACAGAACC AGAAAACGAA 
CCAGTAGAAG AGGCCAAGGG TGACAGCCCC AGCGTTCAAC AAGAGCGAAA ACGAAATGGA 
AAAAAAGGCG GCAAAAGACG AGGAAAAACA GGCGACAGAA GACGCGGAAA AGCTAAAGAG 
TCATCAGATG ACGACACAGA ACCTGTTATT GAACCAGTAA TACAGGACAC ATTAGAAGAA 
ACGAAACCTG TGAACAGTGA AGTATTCGAA GAATTGAAGG GTAACAACCC TGGCGTTGAA 
GTAAAGAGAA CTAGAGGTGA CAGAATCATA GACAATCAAG ATATTGAAGA CACAACACAA 
GAGTCATCTC TGACACCAAC TCAAGTTCCA CCAACAATTG AGACAATACG AAACACGGGA 
TCAACCGATG AGCCAGAGGT CAAAGAGAAA GTTAGCCAGG ACGTAGTAGA GCCGGTCGTG 
AAACCGTTCA ACGAAGAACC TGATAAGTCA GCCACTCTTG AGACCAACGT ACTTGGCGTC 
GCTGCGTTCT TCGATCAAAC CTTGGATATA GATCGTTTAT TACAGCTTCA GTTTGATTTC 
AGTGTCACCA TTAACACCGG TTTCGCTATC CTCTTGACAC AAGGACAAAT TCTTAGGGGA 
GATTATTACT CAATCGGTAT AACCGAGGGA AACGTCTACA TGGTCCAGCG ATGTAATAAG 
GAAACAGCGG AATGTAATCG ACTCTTCAGC AGGAAAATTG ATCCACTTGG TGAAGGAACA 
GGAATATTCA CGCTGAAGAT TCAGAAGATC CTAAATGGCA ATGTAGACCC TGATGCTGAT 
GGAGCATACT CCTACTGGTT TTCATTGCTG TATGAAGGAG CTGTGGTCGG AGAACAGGTG 
GAAGTACAGT ATCCAAATAC ATTCTATATT AGGTACATTG GATGCATCTC CTACAAGTAC 
GTGGCCACGT GGAATCTCTT CTCTGTCGTC TCACCGGGTT CTACCCAGAG ATCCTCTGCA 
ATCATACTCC GGAACGCCGA AGAAACAAGT AGTCTGGAGG TGAATTCATT TGATCGCGAA 
GTTGTATTCA GTGTCAAAGC CCAAGATTTT TTGATAATTC GTCTTTACTC CGTTGGCGAC 
GTCGTCTACA CAATCCTCGT TGGAGACGAA GGCAATAACT ATGTTACGGG ATCGTCCGGT 
ACTGGTGATA AATCTCCGGA TGACGCGTCG CGTCGCGTTG AAGGAGGTGT GTTAAGCTTG 
GCGGGCAAGC AGTACTACCT TTCTTGGCGA AACGGCAGGA TGAAGTTCGG CGAAACGGAG 
GAGGACAGCG AGGCGATGAT GAATTGGGAG ATTGGGGAAA TCTCGGTGAC TAAGCTAGAA 
TTCATTGCTG CTTACGACAG CTTGTATCTC ATACACACAT ACAAGTACTT CTACGTATTT 
GGCGATGTTT TGCAGGCGAC TCATGATCGA ATACGGGCAA CTTCTCTCAC TTTCTTCACT 
CTCCGTCGCG AGCGTGGCGT GCCATACATC CACCTCAAGA TCAAGGCCTC TGGGACTGTC 
TTGATTGCGC TCGCCAAAGA CCAGGAGCTT TCTGAATCCG ACATGGTTTT GATCAAGATG 
AGTTCCCGGA GGATAACTCT CTCCACATGC CTTGGTTGCG AAACAATCTA CTCGGTTCCG 
ATCCCTAAAG ACCAAGCGGC CCTTCTAGAT CCGTCCTCAT TCCTTGAGTA CCAGATCACG 
TGTGAAGGCA ACAGTCTGAA GATCTTCTTA ACCGGTCAGA AGGAACCCTT GCTGGACTAT 
GGTGGCATTG ATCGAGACGC CCACTATGGC TATGATGGCT TCGGAGGAAC TATCGGTAGC 
AATTATCACA TCAAGCTCAA CCAAGGAACG ATCTACAGAG AGGAGCATTG TCTTGACGTC 
AACACCTTCT TGGGTGGAGG AACGAAGCTT CTGAAGATCG TCCACTCTGA CCAGATGATT 
GCTTTATTTG ATGCTGAGAA ACAGCTCGAG GCAGAGCTTG CCATGGAAGA TACGAAGACG 
GAGCTGCGTA CTACCCGTGG TGGCGAGGCC GTTCAGATAA GCCAAAGTAG TAGTTGCATC 
TTCCACGCTC CCTACACTCT AGTCTGGATG GACTTCAGCG GTGGGGCTAT TCGGTATGGT 
ACCTACGACG AGGCGAATCG CCTGAGAAGT GACCCGTGTA TCACCTACGA CCTCGGTGAT 
GATCAGGAGG TCAAATACAT GTCGTTTGGT CAACAGCATT CCTCGTGGGA TGTTACACAT 
CACGTGATTG AGAAGAGGAT TGTGTACAAG GAGTTGGTCT CCCATGTACA AGAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030830438.1
CDS	133..7692
Translation	MNTMNALAFGAVLLLAAVASTSGGGSTSQVADNKERFSYSGGGGLTGLNVKQGGFTQVSHSTKEKTVTTEKETRKSKSKKRKGGKVSTQETSKKETNGGKVKNQRGNTQETSRKRKGRGKNKGNEKTEVKQREGNRGRDGDGKGDRKGRKGSTETVINKERSGGRRRSKNKSGKGSTKGSSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQSVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNAQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDKGSKKDRSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNAQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDKGSKKDRSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNTQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDKGSKKDRSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNTQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRGGEKRERGNTESPQERKRGDRKKGDKDSKSNEKNRGGRRGGSKKDSKSRKGGRNRGRGRWAHRRGKYYRWRTVKGAVKRTRAVSFDERRSAMSVFIKTKATRFVLEFCLSSGGKDCYTASLSSQRDSASFFEYGGQRTGDSQDGAFIRFKRRTRLYVSVSRTKLTIYNDKGDDLSVALPESSVIQDVYMSTSDGDWTKVRFRLRRSGRTLYSSTGPLARGAAFEDIGSKATSFSCLIRTVASAVTFEICFDSQGNDCYTIELSTQPKQTSRILYGGKLVSNEEIGIFITPSKESKIFFRIEKRQLEIWNKEPNNERQLIAKIRGKGRSFNSIWASTSDGEFTELQMVETEAVREFSSTTTLERTSIATQLQFPGQLVLSFALEADKVFMEFRRPDGKSYTIEMGTKAGQPSVIRSGGSNLVDPQDQPFLEFGNDIDPPNQLVIDVFESSVTVSTARGQKMVAPVAEAYLYNHFDIWTVGGGIALAVEEIEDEDSKDSLASKTEIEETEQEIESVEEELKGNNPGVQPVRKRKGKTSGKRRGKTGGRRRGKAKESKSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESKSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESSDDDTEPENEPVEEAKGDSPGIQQERKRKGKIGGKRRGKTGGRIRGKAKESQSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESSDDDTEPVIEPVIQDTLEETKPVNSEVFEELKGNNPGVEVKRTRGDRIIDNQDIEDTTQESSLTPTQVPPTIETIRNTGSTDEPEVKEKVSQDVVEPVVKPFNEEPDKSATLETNVLGVAAFFDQTLDIDRLLQLQFDFSVTINTGFAILLTQGQILRGDYYSIGITEGNVYMVQRCNKETAECNRLFSRKIDPLGEGTGIFTLKIQKILNGNVDPDADGAYSYWFSLLYEGAVVGEQVEVQYPNTFYIRYIGCISYKYVATWNLFSVVSPGSTQRSSAIILRNAEETSSLEVNSFDREVVFSVKAQDFLIIRLYSVGDVVYTILVGDEGNNYVTGSSGTGDKSPDDASRRVEGGVLSLAGKQYYLSWRNGRMKFGETEEDSEAMMNWEIGEISVTKLEFIAAYDSLYLIHTYKYFYVFGDVLQATHDRIRATSLTFFTLRRERGVPYIHLKIKASGTVLIALAKDQELSESDMVLIKMSSRRITLSTCLGCETIYSVPIPKDQAALLDPSSFLEYQITCEGNSLKIFLTGQKEPLLDYGGIDRDAHYGYDGFGGTIGSNYHIKLNQGTIYREEHCLDVNTFLGGGTKLLKIVHSDQMIALFDAEKQLEAELAMEDTKTELRTTRGGEAVQISQSSSCIFHAPYTLVWMDFSGGAIRYGTYDEANRLRSDPCITYDLGDDQEVKYMSFGQQHSSWDVTHHVIEKRIVYKELVSHVQEP

Target ORF information:

RefSeq Version	XM_030974578.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030974578.1

ORF Insert Sequence:

ATGAACACAA TGAACGCCCT GGCGTTTGGC GCTGTTCTGC TGTTAGCTGC AGTCGCGTCG 
ACCTCAGGCG GCGGTAGCAC CAGTCAAGTT GCTGATAATA AAGAGCGATT CTCATACAGC 
GGCGGAGGTG GTCTCACAGG CCTGAACGTC AAGCAAGGTG GTTTTACTCA GGTTTCCCAC 
TCGACAAAAG AAAAGACTGT CACCACGGAG AAAGAAACTA GGAAGAGCAA ATCAAAAAAG 
CGGAAAGGCG GTAAGGTGTC TACTCAAGAG ACTTCAAAGA AAGAAACTAA CGGGGGCAAA 
GTGAAGAACC AGAGAGGCAA TACGCAAGAG ACTTCACGAA AACGAAAAGG AAGGGGAAAG 
AATAAAGGAA ACGAAAAAAC TGAAGTCAAA CAACGAGAAG GAAATCGCGG TCGAGATGGA 
GATGGCAAAG GAGATCGTAA AGGAAGAAAG GGAAGTACTG AGACGGTGAT CAATAAAGAA 
AGGAGCGGTG GTCGGCGTCG AAGTAAAAAC AAGAGTGGTA AGGGATCGAC CAAAGGATCG 
AGCAGTAAAA AGAGTGGAAG AAGAGATTGG AAGATGTCTA AATGCAGAAG CGGGAGACGA 
CAACAGGTTA TTGAGAAACT GGATGAGAAT GAGAGTCTGG AATATGAGTT GACGACCTCT 
GCTGAATCTT TTACCATCCA GTTCTGTCGA AAGAGAGAAA AAGACTGCCT TGAGATTGTT 
CTGAGCTCAT CTGGTGAATC ATCCATTAAG CTTGATGGAC AGGATTTGGG CGATCCCATT 
TCGGACTTCT TTTACAAGAA AGGGGAGAAG TTCTCCTTCA CTATCACCTA TACATCCCAG 
AGTGTGGTTG TGAGGAACAG CAAATCAGGA TTAGAACTTG TTGCATCGGT CGATGGTGAT 
GAACTTAAGG GTTTCAGGTA CTGCTATGGC ACTGCAAACG GAGGAGAAGG AGATTCTACA 
TTCCAGATAA GGGAACGTGA ATTTTCCGAA TCAGAGATCT CATCATTTGA AAAGCGGTAT 
AGGGGCAAGG GAGGTAGAAA GAGGGGTGGA AAGAGAGAAA GGGGAAACGC CCAGTCACCT 
CAAGAAAGGA AGCGTGGCGG TAGGAAGAAA GGCGACAAAG ACTCAAAGTC GAATGAACTT 
TCCGGCGGCA AAAAGAGCAG AGGAAGAGAT AAAGGATCGA AAAAAGACAG GAGCAGTAAA 
AAGAGTGGAA GAAGAGATTG GAAGATGTCT AAATGCAGAA GCGGGAGACG ACAACAGGTT 
ATTGAGAAAC TGGATGAGAA TGAGAGTCTG GAATATGAGT TGACGACCTC TGCTGAATCT 
TTTACCATCC AGTTCTGTCG AAAGAGAGAA AAAGACTGCC TTGAGATTGT TCTGAGCTCA 
TCTGGTGAAT CATCCATTAA GCTTGATGGA CAGGATTTGG GCGATCCCAT TTCGGACTTC 
TTTTACAAGA AAGGGGAGAA GTTCTCCTTC ACTATCACCT ATACATCCCA GCGTGTGGTT 
GTGAGGAACA GCAAATCAGG ATTAGAACTT GTTGCATCGG TCGATGGTGA TGAACTTAAG 
GGTTTTAGGT ACTGCTATGG CACTGCAAAC GGAGGAGAAG GAGATTCTAC ATTCCAGATA 
AGGGAACGTG AATTTTCCGA ATCAGAGATC TCATCATTTG AAAAGCGGTA TAGGGGCAAG 
GGAGGTAGAA AGAGGGGTGG AAAGAGAGAA AGGGGAAACG CCCAGTCACC TCAAGAAAGG 
AAGCGTGGCG GTAGGAAGAA AGGCGACAAA GACTCAAAGT CGAATGAACT TTCCGGCGGC 
AAAAAGAGCA GAGGAAGAGA TAAAGGATCG AAAAAAGACA GGAGCAGTAA AAAGAGTGGA 
AGAAGAGATT GGAAGATGTC TAAATGCAGA AGCGGGAGAC GACAACAGGT TATTGAGAAA 
CTGGATGAGA ATGAGAGTCT GGAATATGAG TTGACGACCT CTGCTGAATC TTTTACCATC 
CAGTTCTGTC GAAAGAGAGA AAAAGACTGC CTTGAGATTG TTCTGAGCTC ATCTGGTGAA 
TCATCCATTA AGCTTGATGG ACAGGATTTG GGCGATCCCA TTTCGGACTT CTTTTACAAG 
AAAGGGGAGA AGTTCTCCTT CACTATCACC TATACATCCC AGCGTGTGGT TGTGAGGAAC 
AGCAAATCAG GATTAGAACT TGTTGCATCG GTCGATGGTG ATGAACTTAA GGGTTTTAGG 
TACTGCTATG GCACTGCAAA CGGAGGAGAA GGAGATTCTA CATTCCAGAT AAGGGAACGT 
GAATTTTCCG AATCAGAGAT CTCATCATTT GAAAAGCGGT ATAGGGGCAA GGGAGGTAGA 
AAGAGGGGTG GAAAGAGAGA AAGGGGAAAC ACCCAGTCAC CCCAAGAAAG GAAGCGTGGC 
GGTAGGAAGA AAGGCGACAA AGACTCAAAG TCGAATGAAC TTTCCGGCGG CAAAAAGAGC 
AGAGGAAGAG ATAAAGGATC GAAAAAAGAC AGGAGCAGTA AAAAGAGTGG AAGAAGAGAT 
TGGAAGATGT CTAAATGCAG AAGCGGGAGA CGACAACAGG TTATTGAGAA ACTGGATGAG 
AATGAGAGTC TGGAATATGA GTTGACGACC TCTGCTGAAT CTTTTACCAT CCAGTTCTGT 
CGAAAGAGAG AAAAAGACTG CCTTGAGATT GTTCTGAGCT CATCTGGTGA ATCATCCATT 
AAGCTTGATG GACAGGATTT GGGCGATCCC ATTTCGGACT TCTTTTACAA GAAAGGGGAG 
AAGTTCTCCT TCACTATCAC CTATACATCC CAGCGTGTGG TTGTGAGGAA CAGCAAATCA 
GGATTAGAAC TTGTTGCATC GGTCGATGGT GATGAACTTA AGGGTTTCAG GTACTGCTAT 
GGCACTGCAA ACGGAGGAGA AGGAGATTCT ACATTCCAGA TAAGGGAACG TGAATTTTCC 
GAATCAGAGA TCTCATCATT TGAAAAGCGG TATAGGGGCA AGGGAGGTAG AAAGAGGGGT 
GGAAAGAGAG AAAGGGGAAA CACCCAGTCA CCCCAAGAAA GGAAGCGTGG CGGTAGGAAG 
AAAGGCGACA AAGACTCAAA GTCGAATGAA CTTTCCGGCG GCAAAAAGAG CAGAGGAAGA 
GGGGGTGAAA AGAGAGAAAG GGGAAACACC GAGTCACCCC AAGAAAGGAA GCGTGGTGAT 
CGTAAAAAAG GCGACAAAGA CTCAAAGTCG AATGAAAAGA ACAGAGGAGG ACGACGTGGA 
GGAAGCAAGA AAGACTCTAA GAGCAGAAAG GGCGGACGAA ATCGAGGTCG TGGAAGATGG 
GCACACCGGA GAGGCAAATA CTATCGATGG CGTACCGTCA AGGGTGCAGT GAAGAGGACA 
CGCGCTGTGA GCTTTGACGA ACGTCGGAGC GCCATGTCTG TTTTTATCAA GACCAAAGCA 
ACTCGATTCG TCTTAGAATT CTGCCTGTCG TCAGGAGGCA AAGACTGCTA TACTGCTTCC 
CTGAGTTCAC AGCGTGATTC GGCCTCCTTC TTCGAGTACG GCGGCCAAAG AACGGGCGAT 
AGTCAAGATG GAGCATTCAT TCGATTTAAA CGAAGAACCA GATTATATGT ATCAGTCTCA 
CGTACTAAAC TGACGATCTA CAATGACAAG GGAGACGATC TCTCAGTCGC TTTACCCGAG 
TCATCAGTCA TCCAAGATGT CTACATGTCC ACATCAGATG GGGACTGGAC GAAGGTTAGA 
TTCCGCCTAC GAAGATCTGG TAGAACCCTT TACAGCTCCA CTGGTCCTCT TGCTCGCGGT 
GCTGCCTTCG AAGATATTGG TTCTAAAGCA ACGTCATTCT CCTGTCTAAT ACGCACCGTG 
GCCAGCGCCG TCACTTTCGA AATTTGCTTC GATTCTCAGG GAAATGATTG TTACACAATC 
GAACTGAGCA CGCAACCCAA ACAGACATCC CGAATCCTGT ATGGAGGAAA ATTAGTGAGC 
AACGAAGAAA TTGGTATTTT TATTACCCCT AGCAAGGAGT CGAAAATCTT CTTTCGCATC 
GAGAAACGGC AGCTGGAGAT CTGGAATAAG GAACCAAACA ATGAACGCCA ACTCATCGCT 
AAAATCCGCG GAAAAGGTCG CAGTTTTAAT TCCATTTGGG CCTCTACTAG TGATGGTGAA 
TTCACCGAGC TTCAGATGGT CGAGACTGAA GCCGTTAGAG AGTTTTCATC CACGACTACT 
CTTGAGAGAA CATCGATTGC CACTCAACTT CAATTCCCAG GGCAACTGGT GTTAAGCTTT 
GCTCTCGAGG CTGACAAGGT CTTCATGGAG TTTCGTCGCC CAGATGGGAA GAGTTATACG 
ATAGAAATGG GTACGAAAGC AGGTCAGCCA TCTGTTATTC GCAGTGGGGG TAGTAACCTA 
GTAGATCCAC AGGACCAACC ATTCCTCGAG TTTGGCAATG ACATTGACCC ACCTAACCAA 
CTCGTCATCG ATGTGTTCGA GTCTAGCGTG ACTGTTTCTA CTGCCCGTGG TCAAAAGATG 
GTGGCACCTG TTGCAGAAGC TTATCTCTAC AACCATTTCG ACATTTGGAC GGTGGGTGGT 
GGTATTGCTC TCGCTGTGGA AGAAATCGAA GATGAAGATT CGAAAGATTC TCTTGCAAGT 
AAGACAGAGA TTGAAGAAAC AGAACAGGAA ATAGAATCAG TAGAAGAGGA ACTTAAGGGT 
AACAACCCCG GCGTTCAACC AGTGCGAAAA CGAAAAGGAA AAACAAGCGG CAAAAGACGA 
GGAAAAACAG GCGGCAGAAG ACGCGGAAAA GCTAAAGAGT CAAAATCAGA TGACGACACA 
GAACCAGAAA ACGAACCAGT AGAAGAGGCC AAGGGTGACA GCCCCAGCGT TCAACAAGAG 
CGAAAACGAA ATGGAAAAAA AGGCGGCAAA AGACGAGGAA AAACAGGCGA CAGAAGACGC 
GGAAAAGCTA AAGAGTCAAA ATCAGATGAC GACACAGAAC CAGAAAACGA ACCAGTAGAA 
GAGGCCAAGG GTGACAGCCC CAGCGTTCAA CAAGAGCGAA AACGAAATGG AAAAAAAGGC 
GGCAAAAGAC GAGGAAAAAC AGGCGACAGA AGACGCGGAA AAGCTAAAGA GTCATCAGAT 
GACGACACAG AACCAGAAAA CGAACCAGTA GAAGAGGCCA AGGGTGACAG CCCCGGCATT 
CAACAAGAGC GAAAACGAAA AGGAAAAATA GGCGGCAAGA GACGAGGAAA AACAGGCGGC 
AGAATACGCG GAAAAGCTAA AGAGTCACAA TCAGATGACG ACACAGAACC AGAAAACGAA 
CCAGTAGAAG AGGCCAAGGG TGACAGCCCC AGCGTTCAAC AAGAGCGAAA ACGAAATGGA 
AAAAAAGGCG GCAAAAGACG AGGAAAAACA GGCGACAGAA GACGCGGAAA AGCTAAAGAG 
TCATCAGATG ACGACACAGA ACCTGTTATT GAACCAGTAA TACAGGACAC ATTAGAAGAA 
ACGAAACCTG TGAACAGTGA AGTATTCGAA GAATTGAAGG GTAACAACCC TGGCGTTGAA 
GTAAAGAGAA CTAGAGGTGA CAGAATCATA GACAATCAAG ATATTGAAGA CACAACACAA 
GAGTCATCTC TGACACCAAC TCAAGTTCCA CCAACAATTG AGACAATACG AAACACGGGA 
TCAACCGATG AGCCAGAGGT CAAAGAGAAA GTTAGCCAGG ACGTAGTAGA GCCGGTCGTG 
AAACCGTTCA ACGAAGAACC TGATAAGTCA GCCACTCTTG AGACCAACGT ACTTGGCGTC 
GCTGCGTTCT TCGATCAAAC CTTGGATATA GATCGTTTAT TACAGCTTCA GTTTGATTTC 
AGTGTCACCA TTAACACCGG TTTCGCTATC CTCTTGACAC AAGGACAAAT TCTTAGGGGA 
GATTATTACT CAATCGGTAT AACCGAGGGA AACGTCTACA TGGTCCAGCG ATGTAATAAG 
GAAACAGCGG AATGTAATCG ACTCTTCAGC AGGAAAATTG ATCCACTTGG TGAAGGAACA 
GGAATATTCA CGCTGAAGAT TCAGAAGATC CTAAATGGCA ATGTAGACCC TGATGCTGAT 
GGAGCATACT CCTACTGGTT TTCATTGCTG TATGAAGGAG CTGTGGTCGG AGAACAGGTG 
GAAGTACAGT ATCCAAATAC ATTCTATATT AGGTACATTG GATGCATCTC CTACAAGTAC 
GTGGCCACGT GGAATCTCTT CTCTGTCGTC TCACCGGGTT CTACCCAGAG ATCCTCTGCA 
ATCATACTCC GGAACGCCGA AGAAACAAGT AGTCTGGAGG TGAATTCATT TGATCGCGAA 
GTTGTATTCA GTGTCAAAGC CCAAGATTTT TTGATAATTC GTCTTTACTC CGTTGGCGAC 
GTCGTCTACA CAATCCTCGT TGGAGACGAA GGCAATAACT ATGTTACGGG ATCGTCCGGT 
ACTGGTGATA AATCTCCGGA TGACGCGTCG CGTCGCGTTG AAGGAGGTGT GTTAAGCTTG 
GCGGGCAAGC AGTACTACCT TTCTTGGCGA AACGGCAGGA TGAAGTTCGG CGAAACGGAG 
GAGGACAGCG AGGCGATGAT GAATTGGGAG ATTGGGGAAA TCTCGGTGAC TAAGCTAGAA 
TTCATTGCTG CTTACGACAG CTTGTATCTC ATACACACAT ACAAGTACTT CTACGTATTT 
GGCGATGTTT TGCAGGCGAC TCATGATCGA ATACGGGCAA CTTCTCTCAC TTTCTTCACT 
CTCCGTCGCG AGCGTGGCGT GCCATACATC CACCTCAAGA TCAAGGCCTC TGGGACTGTC 
TTGATTGCGC TCGCCAAAGA CCAGGAGCTT TCTGAATCCG ACATGGTTTT GATCAAGATG 
AGTTCCCGGA GGATAACTCT CTCCACATGC CTTGGTTGCG AAACAATCTA CTCGGTTCCG 
ATCCCTAAAG ACCAAGCGGC CCTTCTAGAT CCGTCCTCAT TCCTTGAGTA CCAGATCACG 
TGTGAAGGCA ACAGTCTGAA GATCTTCTTA ACCGGTCAGA AGGAACCCTT GCTGGACTAT 
GGTGGCATTG ATCGAGACGC CCACTATGGC TATGATGGCT TCGGAGGAAC TATCGGTAGC 
AATTATCACA TCAAGCTCAA CCAAGGAACG ATCTACAGAG AGGAGCATTG TCTTGACGTC 
AACACCTTCT TGGGTGGAGG AACGAAGCTT CTGAAGATCG TCCACTCTGA CCAGATGATT 
GCTTTATTTG ATGCTGAGAA ACAGCTCGAG GCAGAGCTTG CCATGGAAGA TACGAAGACG 
GAGCTGCGTA CTACCCGTGG TGGCGAGGCC GTTCAGATAA GCCAAAGTAG TAGTTGCATC 
TTCCACGCTC CCTACACTCT AGTCTGGATG GACTTCAGCG GTGGGGCTAT TCGGTATGGT 
ACCTACGACG AGGCGAATCG CCTGAGAAGT GACCCGTGTA TCACCTACGA CCTCGGTGAT 
GATCAGGAGG TCAAATACAT GTCGTTTGGT CAACAGCATT CCTCGTGGGA TGTTACACAT 
CACGTGATTG AGAAGAGGAT TGTGTACAAG GAGTTGGTCT CCCATGTACA AGAGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC576141 ( XM_030974579.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030830439.1 Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X2, mRNA.

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CloneID	OSd69452
Clone ID Related Accession (Same CDS sequence)	XM_030974579.1
Accession Version	XM_030974579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC576141 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAA TGAACGCCCT GGCGTTTGGC GCTGTTCTGC TGTTAGCTGC AGTCGCGTCG 
ACCTCAGGCG GCGGTAGCAC CAGTCAAGTT GCTGATAATA AAGAGCGATT CTCATACAGC 
GGCGGAGGTG GTCTCACAGG CCTGAACGTC AAGCAAGGTG GTTTTACTCA GGTTTCCCAC 
TCGACAAAAG AAAAGACTGT CACCACGGAG AAAGAAACTA GGAAGAGCAA ATCAAAAAAG 
CGGAAAGGCG GTAAGGTGTC TACTCAAGAG ACTTCAAAGA AAGAAACTAA CGGGGGCAAA 
GTGAAGAACC AGAGAGGCAA TACGCAAGAG ACTTCACGAA AACGAAAAGG AAGGGGAAAG 
AATAAAGGAA ACGAAAAAAC TGAAGTCAAA CAACGAGAAG GAAATCGCGG TCGAGATGGA 
GATGGCAAAG GAGATCGTAA AGGAAGAAAG GGAAGTACTG AGACGGTGAT CAATAAAGAA 
AGGAGCGGTG GTCGGCGTCG AAGTAAAAAC AAGAGTGGTA AGGGATCGAC CAAAGGATCG 
AGCAGTAAAA AGAGTGGAAG AAGAGATTGG AAGATGTCTA AATGCAGAAG CGGGAGACGA 
CAACAGGTTA TTGAGAAACT GGATGAGAAT GAGAGTCTGG AATATGAGTT GACGACCTCT 
GCTGAATCTT TTACCATCCA GTTCTGTCGA AAGAGAGAAA AAGACTGCCT TGAGATTGTT 
CTGAGCTCAT CTGGTGAATC ATCCATTAAG CTTGATGGAC AGGATTTGGG CGATCCCATT 
TCGGACTTCT TTTACAAGAA AGGGGAGAAG TTCTCCTTCA CTATCACCTA TACATCCCAG 
AGTGTGGTTG TGAGGAACAG CAAATCAGGA TTAGAACTTG TTGCATCGGT CGATGGTGAT 
GAACTTAAGG GTTTCAGGTA CTGCTATGGC ACTGCAAACG GAGGAGAAGG AGATTCTACA 
TTCCAGATAA GGGAACGTGA ATTTTCCGAA TCAGAGATCT CATCATTTGA AAAGCGGTAT 
AGGGGCAAGG GAGGTAGAAA GAGGGGTGGA AAGAGAGAAA GGGGAAACGC CCAGTCACCT 
CAAGAAAGGA AGCGTGGCGG TAGGAAGAAA GGCGACAAAG ACTCAAAGTC GAATGAACTT 
TCCGGCGGCA AAAAGAGCAG AGGAAGAGAT TGGAAGATGT CTAAATGCAG AAGCGGGAGA 
CGACAACAGG TTATTGAGAA ACTGGATGAG AATGAGAGTC TGGAATATGA GTTGACGACC 
TCTGCTGAAT CTTTTACCAT CCAGTTCTGT CGAAAGAGAG AAAAAGACTG CCTTGAGATT 
GTTCTGAGCT CATCTGGTGA ATCATCCATT AAGCTTGATG GACAGGATTT GGGCGATCCC 
ATTTCGGACT TCTTTTACAA GAAAGGGGAG AAGTTCTCCT TCACTATCAC CTATACATCC 
CAGCGTGTGG TTGTGAGGAA CAGCAAATCA GGATTAGAAC TTGTTGCATC GGTCGATGGT 
GATGAACTTA AGGGTTTTAG GTACTGCTAT GGCACTGCAA ACGGAGGAGA AGGAGATTCT 
ACATTCCAGA TAAGGGAACG TGAATTTTCC GAATCAGAGA TCTCATCATT TGAAAAGCGG 
TATAGGGGCA AGGGAGGTAG AAAGAGGGGT GGAAAGAGAG AAAGGGGAAA CGCCCAGTCA 
CCTCAAGAAA GGAAGCGTGG CGGTAGGAAG AAAGGCGACA AAGACTCAAA GTCGAATGAA 
CTTTCCGGCG GCAAAAAGAG CAGAGGAAGA GATAAAGGAT CGAAAAAAGA CAGGAGCAGT 
AAAAAGAGTG GAAGAAGAGA TTGGAAGATG TCTAAATGCA GAAGCGGGAG ACGACAACAG 
GTTATTGAGA AACTGGATGA GAATGAGAGT CTGGAATATG AGTTGACGAC CTCTGCTGAA 
TCTTTTACCA TCCAGTTCTG TCGAAAGAGA GAAAAAGACT GCCTTGAGAT TGTTCTGAGC 
TCATCTGGTG AATCATCCAT TAAGCTTGAT GGACAGGATT TGGGCGATCC CATTTCGGAC 
TTCTTTTACA AGAAAGGGGA GAAGTTCTCC TTCACTATCA CCTATACATC CCAGCGTGTG 
GTTGTGAGGA ACAGCAAATC AGGATTAGAA CTTGTTGCAT CGGTCGATGG TGATGAACTT 
AAGGGTTTTA GGTACTGCTA TGGCACTGCA AACGGAGGAG AAGGAGATTC TACATTCCAG 
ATAAGGGAAC GTGAATTTTC CGAATCAGAG ATCTCATCAT TTGAAAAGCG GTATAGGGGC 
AAGGGAGGTA GAAAGAGGGG TGGAAAGAGA GAAAGGGGAA ACACCCAGTC ACCCCAAGAA 
AGGAAGCGTG GCGGTAGGAA GAAAGGCGAC AAAGACTCAA AGTCGAATGA ACTTTCCGGC 
GGCAAAAAGA GCAGAGGAAG AGATAAAGGA TCGAAAAAAG ACAGGAGCAG TAAAAAGAGT 
GGAAGAAGAG ATTGGAAGAT GTCTAAATGC AGAAGCGGGA GACGACAACA GGTTATTGAG 
AAACTGGATG AGAATGAGAG TCTGGAATAT GAGTTGACGA CCTCTGCTGA ATCTTTTACC 
ATCCAGTTCT GTCGAAAGAG AGAAAAAGAC TGCCTTGAGA TTGTTCTGAG CTCATCTGGT 
GAATCATCCA TTAAGCTTGA TGGACAGGAT TTGGGCGATC CCATTTCGGA CTTCTTTTAC 
AAGAAAGGGG AGAAGTTCTC CTTCACTATC ACCTATACAT CCCAGCGTGT GGTTGTGAGG 
AACAGCAAAT CAGGATTAGA ACTTGTTGCA TCGGTCGATG GTGATGAACT TAAGGGTTTC 
AGGTACTGCT ATGGCACTGC AAACGGAGGA GAAGGAGATT CTACATTCCA GATAAGGGAA 
CGTGAATTTT CCGAATCAGA GATCTCATCA TTTGAAAAGC GGTATAGGGG CAAGGGAGGT 
AGAAAGAGGG GTGGAAAGAG AGAAAGGGGA AACACCCAGT CACCCCAAGA AAGGAAGCGT 
GGCGGTAGGA AGAAAGGCGA CAAAGACTCA AAGTCGAATG AACTTTCCGG CGGCAAAAAG 
AGCAGAGGAA GAGGGGGTGA AAAGAGAGAA AGGGGAAACA CCGAGTCACC CCAAGAAAGG 
AAGCGTGGTG ATCGTAAAAA AGGCGACAAA GACTCAAAGT CGAATGAAAA GAACAGAGGA 
GGACGACGTG GAGGAAGCAA GAAAGACTCT AAGAGCAGAA AGGGCGGACG AAATCGAGGT 
CGTGGAAGAT GGGCACACCG GAGAGGCAAA TACTATCGAT GGCGTACCGT CAAGGGTGCA 
GTGAAGAGGA CACGCGCTGT GAGCTTTGAC GAACGTCGGA GCGCCATGTC TGTTTTTATC 
AAGACCAAAG CAACTCGATT CGTCTTAGAA TTCTGCCTGT CGTCAGGAGG CAAAGACTGC 
TATACTGCTT CCCTGAGTTC ACAGCGTGAT TCGGCCTCCT TCTTCGAGTA CGGCGGCCAA 
AGAACGGGCG ATAGTCAAGA TGGAGCATTC ATTCGATTTA AACGAAGAAC CAGATTATAT 
GTATCAGTCT CACGTACTAA ACTGACGATC TACAATGACA AGGGAGACGA TCTCTCAGTC 
GCTTTACCCG AGTCATCAGT CATCCAAGAT GTCTACATGT CCACATCAGA TGGGGACTGG 
ACGAAGGTTA GATTCCGCCT ACGAAGATCT GGTAGAACCC TTTACAGCTC CACTGGTCCT 
CTTGCTCGCG GTGCTGCCTT CGAAGATATT GGTTCTAAAG CAACGTCATT CTCCTGTCTA 
ATACGCACCG TGGCCAGCGC CGTCACTTTC GAAATTTGCT TCGATTCTCA GGGAAATGAT 
TGTTACACAA TCGAACTGAG CACGCAACCC AAACAGACAT CCCGAATCCT GTATGGAGGA 
AAATTAGTGA GCAACGAAGA AATTGGTATT TTTATTACCC CTAGCAAGGA GTCGAAAATC 
TTCTTTCGCA TCGAGAAACG GCAGCTGGAG ATCTGGAATA AGGAACCAAA CAATGAACGC 
CAACTCATCG CTAAAATCCG CGGAAAAGGT CGCAGTTTTA ATTCCATTTG GGCCTCTACT 
AGTGATGGTG AATTCACCGA GCTTCAGATG GTCGAGACTG AAGCCGTTAG AGAGTTTTCA 
TCCACGACTA CTCTTGAGAG AACATCGATT GCCACTCAAC TTCAATTCCC AGGGCAACTG 
GTGTTAAGCT TTGCTCTCGA GGCTGACAAG GTCTTCATGG AGTTTCGTCG CCCAGATGGG 
AAGAGTTATA CGATAGAAAT GGGTACGAAA GCAGGTCAGC CATCTGTTAT TCGCAGTGGG 
GGTAGTAACC TAGTAGATCC ACAGGACCAA CCATTCCTCG AGTTTGGCAA TGACATTGAC 
CCACCTAACC AACTCGTCAT CGATGTGTTC GAGTCTAGCG TGACTGTTTC TACTGCCCGT 
GGTCAAAAGA TGGTGGCACC TGTTGCAGAA GCTTATCTCT ACAACCATTT CGACATTTGG 
ACGGTGGGTG GTGGTATTGC TCTCGCTGTG GAAGAAATCG AAGATGAAGA TTCGAAAGAT 
TCTCTTGCAA GTAAGACAGA GATTGAAGAA ACAGAACAGG AAATAGAATC AGTAGAAGAG 
GAACTTAAGG GTAACAACCC CGGCGTTCAA CCAGTGCGAA AACGAAAAGG AAAAACAAGC 
GGCAAAAGAC GAGGAAAAAC AGGCGGCAGA AGACGCGGAA AAGCTAAAGA GTCAAAATCA 
GATGACGACA CAGAACCAGA AAACGAACCA GTAGAAGAGG CCAAGGGTGA CAGCCCCAGC 
GTTCAACAAG AGCGAAAACG AAATGGAAAA AAAGGCGGCA AAAGACGAGG AAAAACAGGC 
GACAGAAGAC GCGGAAAAGC TAAAGAGTCA AAATCAGATG ACGACACAGA ACCAGAAAAC 
GAACCAGTAG AAGAGGCCAA GGGTGACAGC CCCAGCGTTC AACAAGAGCG AAAACGAAAT 
GGAAAAAAAG GCGGCAAAAG ACGAGGAAAA ACAGGCGACA GAAGACGCGG AAAAGCTAAA 
GAGTCATCAG ATGACGACAC AGAACCAGAA AACGAACCAG TAGAAGAGGC CAAGGGTGAC 
AGCCCCGGCA TTCAACAAGA GCGAAAACGA AAAGGAAAAA TAGGCGGCAA GAGACGAGGA 
AAAACAGGCG GCAGAATACG CGGAAAAGCT AAAGAGTCAC AATCAGATGA CGACACAGAA 
CCAGAAAACG AACCAGTAGA AGAGGCCAAG GGTGACAGCC CCAGCGTTCA ACAAGAGCGA 
AAACGAAATG GAAAAAAAGG CGGCAAAAGA CGAGGAAAAA CAGGCGACAG AAGACGCGGA 
AAAGCTAAAG AGTCATCAGA TGACGACACA GAACCTGTTA TTGAACCAGT AATACAGGAC 
ACATTAGAAG AAACGAAACC TGTGAACAGT GAAGTATTCG AAGAATTGAA GGGTAACAAC 
CCTGGCGTTG AAGTAAAGAG AACTAGAGGT GACAGAATCA TAGACAATCA AGATATTGAA 
GACACAACAC AAGAGTCATC TCTGACACCA ACTCAAGTTC CACCAACAAT TGAGACAATA 
CGAAACACGG GATCAACCGA TGAGCCAGAG GTCAAAGAGA AAGTTAGCCA GGACGTAGTA 
GAGCCGGTCG TGAAACCGTT CAACGAAGAA CCTGATAAGT CAGCCACTCT TGAGACCAAC 
GTACTTGGCG TCGCTGCGTT CTTCGATCAA ACCTTGGATA TAGATCGTTT ATTACAGCTT 
CAGTTTGATT TCAGTGTCAC CATTAACACC GGTTTCGCTA TCCTCTTGAC ACAAGGACAA 
ATTCTTAGGG GAGATTATTA CTCAATCGGT ATAACCGAGG GAAACGTCTA CATGGTCCAG 
CGATGTAATA AGGAAACAGC GGAATGTAAT CGACTCTTCA GCAGGAAAAT TGATCCACTT 
GGTGAAGGAA CAGGAATATT CACGCTGAAG ATTCAGAAGA TCCTAAATGG CAATGTAGAC 
CCTGATGCTG ATGGAGCATA CTCCTACTGG TTTTCATTGC TGTATGAAGG AGCTGTGGTC 
GGAGAACAGG TGGAAGTACA GTATCCAAAT ACATTCTATA TTAGGTACAT TGGATGCATC 
TCCTACAAGT ACGTGGCCAC GTGGAATCTC TTCTCTGTCG TCTCACCGGG TTCTACCCAG 
AGATCCTCTG CAATCATACT CCGGAACGCC GAAGAAACAA GTAGTCTGGA GGTGAATTCA 
TTTGATCGCG AAGTTGTATT CAGTGTCAAA GCCCAAGATT TTTTGATAAT TCGTCTTTAC 
TCCGTTGGCG ACGTCGTCTA CACAATCCTC GTTGGAGACG AAGGCAATAA CTATGTTACG 
GGATCGTCCG GTACTGGTGA TAAATCTCCG GATGACGCGT CGCGTCGCGT TGAAGGAGGT 
GTGTTAAGCT TGGCGGGCAA GCAGTACTAC CTTTCTTGGC GAAACGGCAG GATGAAGTTC 
GGCGAAACGG AGGAGGACAG CGAGGCGATG ATGAATTGGG AGATTGGGGA AATCTCGGTG 
ACTAAGCTAG AATTCATTGC TGCTTACGAC AGCTTGTATC TCATACACAC ATACAAGTAC 
TTCTACGTAT TTGGCGATGT TTTGCAGGCG ACTCATGATC GAATACGGGC AACTTCTCTC 
ACTTTCTTCA CTCTCCGTCG CGAGCGTGGC GTGCCATACA TCCACCTCAA GATCAAGGCC 
TCTGGGACTG TCTTGATTGC GCTCGCCAAA GACCAGGAGC TTTCTGAATC CGACATGGTT 
TTGATCAAGA TGAGTTCCCG GAGGATAACT CTCTCCACAT GCCTTGGTTG CGAAACAATC 
TACTCGGTTC CGATCCCTAA AGACCAAGCG GCCCTTCTAG ATCCGTCCTC ATTCCTTGAG 
TACCAGATCA CGTGTGAAGG CAACAGTCTG AAGATCTTCT TAACCGGTCA GAAGGAACCC 
TTGCTGGACT ATGGTGGCAT TGATCGAGAC GCCCACTATG GCTATGATGG CTTCGGAGGA 
ACTATCGGTA GCAATTATCA CATCAAGCTC AACCAAGGAA CGATCTACAG AGAGGAGCAT 
TGTCTTGACG TCAACACCTT CTTGGGTGGA GGAACGAAGC TTCTGAAGAT CGTCCACTCT 
GACCAGATGA TTGCTTTATT TGATGCTGAG AAACAGCTCG AGGCAGAGCT TGCCATGGAA 
GATACGAAGA CGGAGCTGCG TACTACCCGT GGTGGCGAGG CCGTTCAGAT AAGCCAAAGT 
AGTAGTTGCA TCTTCCACGC TCCCTACACT CTAGTCTGGA TGGACTTCAG CGGTGGGGCT 
ATTCGGTATG GTACCTACGA CGAGGCGAAT CGCCTGAGAA GTGACCCGTG TATCACCTAC 
GACCTCGGTG ATGATCAGGA GGTCAAATAC ATGTCGTTTG GTCAACAGCA TTCCTCGTGG 
GATGTTACAC ATCACGTGAT TGAGAAGAGG ATTGTGTACA AGGAGTTGGT CTCCCATGTA 
CAAGAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030830439.1
CDS	133..7644
Translation	MNTMNALAFGAVLLLAAVASTSGGGSTSQVADNKERFSYSGGGGLTGLNVKQGGFTQVSHSTKEKTVTTEKETRKSKSKKRKGGKVSTQETSKKETNGGKVKNQRGNTQETSRKRKGRGKNKGNEKTEVKQREGNRGRDGDGKGDRKGRKGSTETVINKERSGGRRRSKNKSGKGSTKGSSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQSVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNAQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNAQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDKGSKKDRSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNTQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDKGSKKDRSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNTQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRGGEKRERGNTESPQERKRGDRKKGDKDSKSNEKNRGGRRGGSKKDSKSRKGGRNRGRGRWAHRRGKYYRWRTVKGAVKRTRAVSFDERRSAMSVFIKTKATRFVLEFCLSSGGKDCYTASLSSQRDSASFFEYGGQRTGDSQDGAFIRFKRRTRLYVSVSRTKLTIYNDKGDDLSVALPESSVIQDVYMSTSDGDWTKVRFRLRRSGRTLYSSTGPLARGAAFEDIGSKATSFSCLIRTVASAVTFEICFDSQGNDCYTIELSTQPKQTSRILYGGKLVSNEEIGIFITPSKESKIFFRIEKRQLEIWNKEPNNERQLIAKIRGKGRSFNSIWASTSDGEFTELQMVETEAVREFSSTTTLERTSIATQLQFPGQLVLSFALEADKVFMEFRRPDGKSYTIEMGTKAGQPSVIRSGGSNLVDPQDQPFLEFGNDIDPPNQLVIDVFESSVTVSTARGQKMVAPVAEAYLYNHFDIWTVGGGIALAVEEIEDEDSKDSLASKTEIEETEQEIESVEEELKGNNPGVQPVRKRKGKTSGKRRGKTGGRRRGKAKESKSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESKSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESSDDDTEPENEPVEEAKGDSPGIQQERKRKGKIGGKRRGKTGGRIRGKAKESQSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESSDDDTEPVIEPVIQDTLEETKPVNSEVFEELKGNNPGVEVKRTRGDRIIDNQDIEDTTQESSLTPTQVPPTIETIRNTGSTDEPEVKEKVSQDVVEPVVKPFNEEPDKSATLETNVLGVAAFFDQTLDIDRLLQLQFDFSVTINTGFAILLTQGQILRGDYYSIGITEGNVYMVQRCNKETAECNRLFSRKIDPLGEGTGIFTLKIQKILNGNVDPDADGAYSYWFSLLYEGAVVGEQVEVQYPNTFYIRYIGCISYKYVATWNLFSVVSPGSTQRSSAIILRNAEETSSLEVNSFDREVVFSVKAQDFLIIRLYSVGDVVYTILVGDEGNNYVTGSSGTGDKSPDDASRRVEGGVLSLAGKQYYLSWRNGRMKFGETEEDSEAMMNWEIGEISVTKLEFIAAYDSLYLIHTYKYFYVFGDVLQATHDRIRATSLTFFTLRRERGVPYIHLKIKASGTVLIALAKDQELSESDMVLIKMSSRRITLSTCLGCETIYSVPIPKDQAALLDPSSFLEYQITCEGNSLKIFLTGQKEPLLDYGGIDRDAHYGYDGFGGTIGSNYHIKLNQGTIYREEHCLDVNTFLGGGTKLLKIVHSDQMIALFDAEKQLEAELAMEDTKTELRTTRGGEAVQISQSSSCIFHAPYTLVWMDFSGGAIRYGTYDEANRLRSDPCITYDLGDDQEVKYMSFGQQHSSWDVTHHVIEKRIVYKELVSHVQEP

Target ORF information:

RefSeq Version	XM_030974579.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030974579.1

ORF Insert Sequence:

ATGAACACAA TGAACGCCCT GGCGTTTGGC GCTGTTCTGC TGTTAGCTGC AGTCGCGTCG 
ACCTCAGGCG GCGGTAGCAC CAGTCAAGTT GCTGATAATA AAGAGCGATT CTCATACAGC 
GGCGGAGGTG GTCTCACAGG CCTGAACGTC AAGCAAGGTG GTTTTACTCA GGTTTCCCAC 
TCGACAAAAG AAAAGACTGT CACCACGGAG AAAGAAACTA GGAAGAGCAA ATCAAAAAAG 
CGGAAAGGCG GTAAGGTGTC TACTCAAGAG ACTTCAAAGA AAGAAACTAA CGGGGGCAAA 
GTGAAGAACC AGAGAGGCAA TACGCAAGAG ACTTCACGAA AACGAAAAGG AAGGGGAAAG 
AATAAAGGAA ACGAAAAAAC TGAAGTCAAA CAACGAGAAG GAAATCGCGG TCGAGATGGA 
GATGGCAAAG GAGATCGTAA AGGAAGAAAG GGAAGTACTG AGACGGTGAT CAATAAAGAA 
AGGAGCGGTG GTCGGCGTCG AAGTAAAAAC AAGAGTGGTA AGGGATCGAC CAAAGGATCG 
AGCAGTAAAA AGAGTGGAAG AAGAGATTGG AAGATGTCTA AATGCAGAAG CGGGAGACGA 
CAACAGGTTA TTGAGAAACT GGATGAGAAT GAGAGTCTGG AATATGAGTT GACGACCTCT 
GCTGAATCTT TTACCATCCA GTTCTGTCGA AAGAGAGAAA AAGACTGCCT TGAGATTGTT 
CTGAGCTCAT CTGGTGAATC ATCCATTAAG CTTGATGGAC AGGATTTGGG CGATCCCATT 
TCGGACTTCT TTTACAAGAA AGGGGAGAAG TTCTCCTTCA CTATCACCTA TACATCCCAG 
AGTGTGGTTG TGAGGAACAG CAAATCAGGA TTAGAACTTG TTGCATCGGT CGATGGTGAT 
GAACTTAAGG GTTTCAGGTA CTGCTATGGC ACTGCAAACG GAGGAGAAGG AGATTCTACA 
TTCCAGATAA GGGAACGTGA ATTTTCCGAA TCAGAGATCT CATCATTTGA AAAGCGGTAT 
AGGGGCAAGG GAGGTAGAAA GAGGGGTGGA AAGAGAGAAA GGGGAAACGC CCAGTCACCT 
CAAGAAAGGA AGCGTGGCGG TAGGAAGAAA GGCGACAAAG ACTCAAAGTC GAATGAACTT 
TCCGGCGGCA AAAAGAGCAG AGGAAGAGAT TGGAAGATGT CTAAATGCAG AAGCGGGAGA 
CGACAACAGG TTATTGAGAA ACTGGATGAG AATGAGAGTC TGGAATATGA GTTGACGACC 
TCTGCTGAAT CTTTTACCAT CCAGTTCTGT CGAAAGAGAG AAAAAGACTG CCTTGAGATT 
GTTCTGAGCT CATCTGGTGA ATCATCCATT AAGCTTGATG GACAGGATTT GGGCGATCCC 
ATTTCGGACT TCTTTTACAA GAAAGGGGAG AAGTTCTCCT TCACTATCAC CTATACATCC 
CAGCGTGTGG TTGTGAGGAA CAGCAAATCA GGATTAGAAC TTGTTGCATC GGTCGATGGT 
GATGAACTTA AGGGTTTTAG GTACTGCTAT GGCACTGCAA ACGGAGGAGA AGGAGATTCT 
ACATTCCAGA TAAGGGAACG TGAATTTTCC GAATCAGAGA TCTCATCATT TGAAAAGCGG 
TATAGGGGCA AGGGAGGTAG AAAGAGGGGT GGAAAGAGAG AAAGGGGAAA CGCCCAGTCA 
CCTCAAGAAA GGAAGCGTGG CGGTAGGAAG AAAGGCGACA AAGACTCAAA GTCGAATGAA 
CTTTCCGGCG GCAAAAAGAG CAGAGGAAGA GATAAAGGAT CGAAAAAAGA CAGGAGCAGT 
AAAAAGAGTG GAAGAAGAGA TTGGAAGATG TCTAAATGCA GAAGCGGGAG ACGACAACAG 
GTTATTGAGA AACTGGATGA GAATGAGAGT CTGGAATATG AGTTGACGAC CTCTGCTGAA 
TCTTTTACCA TCCAGTTCTG TCGAAAGAGA GAAAAAGACT GCCTTGAGAT TGTTCTGAGC 
TCATCTGGTG AATCATCCAT TAAGCTTGAT GGACAGGATT TGGGCGATCC CATTTCGGAC 
TTCTTTTACA AGAAAGGGGA GAAGTTCTCC TTCACTATCA CCTATACATC CCAGCGTGTG 
GTTGTGAGGA ACAGCAAATC AGGATTAGAA CTTGTTGCAT CGGTCGATGG TGATGAACTT 
AAGGGTTTTA GGTACTGCTA TGGCACTGCA AACGGAGGAG AAGGAGATTC TACATTCCAG 
ATAAGGGAAC GTGAATTTTC CGAATCAGAG ATCTCATCAT TTGAAAAGCG GTATAGGGGC 
AAGGGAGGTA GAAAGAGGGG TGGAAAGAGA GAAAGGGGAA ACACCCAGTC ACCCCAAGAA 
AGGAAGCGTG GCGGTAGGAA GAAAGGCGAC AAAGACTCAA AGTCGAATGA ACTTTCCGGC 
GGCAAAAAGA GCAGAGGAAG AGATAAAGGA TCGAAAAAAG ACAGGAGCAG TAAAAAGAGT 
GGAAGAAGAG ATTGGAAGAT GTCTAAATGC AGAAGCGGGA GACGACAACA GGTTATTGAG 
AAACTGGATG AGAATGAGAG TCTGGAATAT GAGTTGACGA CCTCTGCTGA ATCTTTTACC 
ATCCAGTTCT GTCGAAAGAG AGAAAAAGAC TGCCTTGAGA TTGTTCTGAG CTCATCTGGT 
GAATCATCCA TTAAGCTTGA TGGACAGGAT TTGGGCGATC CCATTTCGGA CTTCTTTTAC 
AAGAAAGGGG AGAAGTTCTC CTTCACTATC ACCTATACAT CCCAGCGTGT GGTTGTGAGG 
AACAGCAAAT CAGGATTAGA ACTTGTTGCA TCGGTCGATG GTGATGAACT TAAGGGTTTC 
AGGTACTGCT ATGGCACTGC AAACGGAGGA GAAGGAGATT CTACATTCCA GATAAGGGAA 
CGTGAATTTT CCGAATCAGA GATCTCATCA TTTGAAAAGC GGTATAGGGG CAAGGGAGGT 
AGAAAGAGGG GTGGAAAGAG AGAAAGGGGA AACACCCAGT CACCCCAAGA AAGGAAGCGT 
GGCGGTAGGA AGAAAGGCGA CAAAGACTCA AAGTCGAATG AACTTTCCGG CGGCAAAAAG 
AGCAGAGGAA GAGGGGGTGA AAAGAGAGAA AGGGGAAACA CCGAGTCACC CCAAGAAAGG 
AAGCGTGGTG ATCGTAAAAA AGGCGACAAA GACTCAAAGT CGAATGAAAA GAACAGAGGA 
GGACGACGTG GAGGAAGCAA GAAAGACTCT AAGAGCAGAA AGGGCGGACG AAATCGAGGT 
CGTGGAAGAT GGGCACACCG GAGAGGCAAA TACTATCGAT GGCGTACCGT CAAGGGTGCA 
GTGAAGAGGA CACGCGCTGT GAGCTTTGAC GAACGTCGGA GCGCCATGTC TGTTTTTATC 
AAGACCAAAG CAACTCGATT CGTCTTAGAA TTCTGCCTGT CGTCAGGAGG CAAAGACTGC 
TATACTGCTT CCCTGAGTTC ACAGCGTGAT TCGGCCTCCT TCTTCGAGTA CGGCGGCCAA 
AGAACGGGCG ATAGTCAAGA TGGAGCATTC ATTCGATTTA AACGAAGAAC CAGATTATAT 
GTATCAGTCT CACGTACTAA ACTGACGATC TACAATGACA AGGGAGACGA TCTCTCAGTC 
GCTTTACCCG AGTCATCAGT CATCCAAGAT GTCTACATGT CCACATCAGA TGGGGACTGG 
ACGAAGGTTA GATTCCGCCT ACGAAGATCT GGTAGAACCC TTTACAGCTC CACTGGTCCT 
CTTGCTCGCG GTGCTGCCTT CGAAGATATT GGTTCTAAAG CAACGTCATT CTCCTGTCTA 
ATACGCACCG TGGCCAGCGC CGTCACTTTC GAAATTTGCT TCGATTCTCA GGGAAATGAT 
TGTTACACAA TCGAACTGAG CACGCAACCC AAACAGACAT CCCGAATCCT GTATGGAGGA 
AAATTAGTGA GCAACGAAGA AATTGGTATT TTTATTACCC CTAGCAAGGA GTCGAAAATC 
TTCTTTCGCA TCGAGAAACG GCAGCTGGAG ATCTGGAATA AGGAACCAAA CAATGAACGC 
CAACTCATCG CTAAAATCCG CGGAAAAGGT CGCAGTTTTA ATTCCATTTG GGCCTCTACT 
AGTGATGGTG AATTCACCGA GCTTCAGATG GTCGAGACTG AAGCCGTTAG AGAGTTTTCA 
TCCACGACTA CTCTTGAGAG AACATCGATT GCCACTCAAC TTCAATTCCC AGGGCAACTG 
GTGTTAAGCT TTGCTCTCGA GGCTGACAAG GTCTTCATGG AGTTTCGTCG CCCAGATGGG 
AAGAGTTATA CGATAGAAAT GGGTACGAAA GCAGGTCAGC CATCTGTTAT TCGCAGTGGG 
GGTAGTAACC TAGTAGATCC ACAGGACCAA CCATTCCTCG AGTTTGGCAA TGACATTGAC 
CCACCTAACC AACTCGTCAT CGATGTGTTC GAGTCTAGCG TGACTGTTTC TACTGCCCGT 
GGTCAAAAGA TGGTGGCACC TGTTGCAGAA GCTTATCTCT ACAACCATTT CGACATTTGG 
ACGGTGGGTG GTGGTATTGC TCTCGCTGTG GAAGAAATCG AAGATGAAGA TTCGAAAGAT 
TCTCTTGCAA GTAAGACAGA GATTGAAGAA ACAGAACAGG AAATAGAATC AGTAGAAGAG 
GAACTTAAGG GTAACAACCC CGGCGTTCAA CCAGTGCGAA AACGAAAAGG AAAAACAAGC 
GGCAAAAGAC GAGGAAAAAC AGGCGGCAGA AGACGCGGAA AAGCTAAAGA GTCAAAATCA 
GATGACGACA CAGAACCAGA AAACGAACCA GTAGAAGAGG CCAAGGGTGA CAGCCCCAGC 
GTTCAACAAG AGCGAAAACG AAATGGAAAA AAAGGCGGCA AAAGACGAGG AAAAACAGGC 
GACAGAAGAC GCGGAAAAGC TAAAGAGTCA AAATCAGATG ACGACACAGA ACCAGAAAAC 
GAACCAGTAG AAGAGGCCAA GGGTGACAGC CCCAGCGTTC AACAAGAGCG AAAACGAAAT 
GGAAAAAAAG GCGGCAAAAG ACGAGGAAAA ACAGGCGACA GAAGACGCGG AAAAGCTAAA 
GAGTCATCAG ATGACGACAC AGAACCAGAA AACGAACCAG TAGAAGAGGC CAAGGGTGAC 
AGCCCCGGCA TTCAACAAGA GCGAAAACGA AAAGGAAAAA TAGGCGGCAA GAGACGAGGA 
AAAACAGGCG GCAGAATACG CGGAAAAGCT AAAGAGTCAC AATCAGATGA CGACACAGAA 
CCAGAAAACG AACCAGTAGA AGAGGCCAAG GGTGACAGCC CCAGCGTTCA ACAAGAGCGA 
AAACGAAATG GAAAAAAAGG CGGCAAAAGA CGAGGAAAAA CAGGCGACAG AAGACGCGGA 
AAAGCTAAAG AGTCATCAGA TGACGACACA GAACCTGTTA TTGAACCAGT AATACAGGAC 
ACATTAGAAG AAACGAAACC TGTGAACAGT GAAGTATTCG AAGAATTGAA GGGTAACAAC 
CCTGGCGTTG AAGTAAAGAG AACTAGAGGT GACAGAATCA TAGACAATCA AGATATTGAA 
GACACAACAC AAGAGTCATC TCTGACACCA ACTCAAGTTC CACCAACAAT TGAGACAATA 
CGAAACACGG GATCAACCGA TGAGCCAGAG GTCAAAGAGA AAGTTAGCCA GGACGTAGTA 
GAGCCGGTCG TGAAACCGTT CAACGAAGAA CCTGATAAGT CAGCCACTCT TGAGACCAAC 
GTACTTGGCG TCGCTGCGTT CTTCGATCAA ACCTTGGATA TAGATCGTTT ATTACAGCTT 
CAGTTTGATT TCAGTGTCAC CATTAACACC GGTTTCGCTA TCCTCTTGAC ACAAGGACAA 
ATTCTTAGGG GAGATTATTA CTCAATCGGT ATAACCGAGG GAAACGTCTA CATGGTCCAG 
CGATGTAATA AGGAAACAGC GGAATGTAAT CGACTCTTCA GCAGGAAAAT TGATCCACTT 
GGTGAAGGAA CAGGAATATT CACGCTGAAG ATTCAGAAGA TCCTAAATGG CAATGTAGAC 
CCTGATGCTG ATGGAGCATA CTCCTACTGG TTTTCATTGC TGTATGAAGG AGCTGTGGTC 
GGAGAACAGG TGGAAGTACA GTATCCAAAT ACATTCTATA TTAGGTACAT TGGATGCATC 
TCCTACAAGT ACGTGGCCAC GTGGAATCTC TTCTCTGTCG TCTCACCGGG TTCTACCCAG 
AGATCCTCTG CAATCATACT CCGGAACGCC GAAGAAACAA GTAGTCTGGA GGTGAATTCA 
TTTGATCGCG AAGTTGTATT CAGTGTCAAA GCCCAAGATT TTTTGATAAT TCGTCTTTAC 
TCCGTTGGCG ACGTCGTCTA CACAATCCTC GTTGGAGACG AAGGCAATAA CTATGTTACG 
GGATCGTCCG GTACTGGTGA TAAATCTCCG GATGACGCGT CGCGTCGCGT TGAAGGAGGT 
GTGTTAAGCT TGGCGGGCAA GCAGTACTAC CTTTCTTGGC GAAACGGCAG GATGAAGTTC 
GGCGAAACGG AGGAGGACAG CGAGGCGATG ATGAATTGGG AGATTGGGGA AATCTCGGTG 
ACTAAGCTAG AATTCATTGC TGCTTACGAC AGCTTGTATC TCATACACAC ATACAAGTAC 
TTCTACGTAT TTGGCGATGT TTTGCAGGCG ACTCATGATC GAATACGGGC AACTTCTCTC 
ACTTTCTTCA CTCTCCGTCG CGAGCGTGGC GTGCCATACA TCCACCTCAA GATCAAGGCC 
TCTGGGACTG TCTTGATTGC GCTCGCCAAA GACCAGGAGC TTTCTGAATC CGACATGGTT 
TTGATCAAGA TGAGTTCCCG GAGGATAACT CTCTCCACAT GCCTTGGTTG CGAAACAATC 
TACTCGGTTC CGATCCCTAA AGACCAAGCG GCCCTTCTAG ATCCGTCCTC ATTCCTTGAG 
TACCAGATCA CGTGTGAAGG CAACAGTCTG AAGATCTTCT TAACCGGTCA GAAGGAACCC 
TTGCTGGACT ATGGTGGCAT TGATCGAGAC GCCCACTATG GCTATGATGG CTTCGGAGGA 
ACTATCGGTA GCAATTATCA CATCAAGCTC AACCAAGGAA CGATCTACAG AGAGGAGCAT 
TGTCTTGACG TCAACACCTT CTTGGGTGGA GGAACGAAGC TTCTGAAGAT CGTCCACTCT 
GACCAGATGA TTGCTTTATT TGATGCTGAG AAACAGCTCG AGGCAGAGCT TGCCATGGAA 
GATACGAAGA CGGAGCTGCG TACTACCCGT GGTGGCGAGG CCGTTCAGAT AAGCCAAAGT 
AGTAGTTGCA TCTTCCACGC TCCCTACACT CTAGTCTGGA TGGACTTCAG CGGTGGGGCT 
ATTCGGTATG GTACCTACGA CGAGGCGAAT CGCCTGAGAA GTGACCCGTG TATCACCTAC 
GACCTCGGTG ATGATCAGGA GGTCAAATAC ATGTCGTTTG GTCAACAGCA TTCCTCGTGG 
GATGTTACAC ATCACGTGAT TGAGAAGAGG ATTGTGTACA AGGAGTTGGT CTCCCATGTA 
CAAGAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC576141 ( XM_030974580.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030830440.1 Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X3, mRNA.

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CloneID	OSd69453
Clone ID Related Accession (Same CDS sequence)	XM_030974580.1
Accession Version	XM_030974580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC576141 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAA TGAACGCCCT GGCGTTTGGC GCTGTTCTGC TGTTAGCTGC AGTCGCGTCG 
ACCTCAGGCG GCGGTAGCAC CAGTCAAGTT GCTGATAATA AAGAGCGATT CTCATACAGC 
GGCGGAGGTG GTCTCACAGG CCTGAACGTC AAGCAAGGTG GTTTTACTCA GGTTTCCCAC 
TCGACAAAAG AAAAGACTGT CACCACGGAG AAAGAAACTA GGAAGAGCAA ATCAAAAAAG 
CGGAAAGGCG GTAAGGTGTC TACTCAAGAG ACTTCAAAGA AAGAAACTAA CGGGGGCAAA 
GTGAAGAACC AGAGAGGCAA TACGCAAGAG ACTTCACGAA AACGAAAAGG AAGGGGAAAG 
AATAAAGGAA ACGAAAAAAC TGAAGTCAAA CAACGAGAAG GAAATCGCGG TCGAGATGGA 
GATGGCAAAG GAGATCGTAA AGGAAGAAAG GGAAGTACTG AGACGGTGAT CAATAAAGAA 
AGGAGCGGTG GTCGGCGTCG AAGTAAAAAC AAGAGTGGTA AGGGATCGAC CAAAGGATCG 
AGCAGTAAAA AGAGTGGAAG AAGAGATTGG AAGATGTCTA AATGCAGAAG CGGGAGACGA 
CAACAGGTTA TTGAGAAACT GGATGAGAAT GAGAGTCTGG AATATGAGTT GACGACCTCT 
GCTGAATCTT TTACCATCCA GTTCTGTCGA AAGAGAGAAA AAGACTGCCT TGAGATTGTT 
CTGAGCTCAT CTGGTGAATC ATCCATTAAG CTTGATGGAC AGGATTTGGG CGATCCCATT 
TCGGACTTCT TTTACAAGAA AGGGGAGAAG TTCTCCTTCA CTATCACCTA TACATCCCAG 
AGTGTGGTTG TGAGGAACAG CAAATCAGGA TTAGAACTTG TTGCATCGGT CGATGGTGAT 
GAACTTAAGG GTTTCAGGTA CTGCTATGGC ACTGCAAACG GAGGAGAAGG AGATTCTACA 
TTCCAGATAA GGGAACGTGA ATTTTCCGAA TCAGAGATCT CATCATTTGA AAAGCGGTAT 
AGGGGCAAGG GAGGTAGAAA GAGGGGTGGA AAGAGAGAAA GGGGAAACGC CCAGTCACCT 
CAAGAAAGGA AGCGTGGCGG TAGGAAGAAA GGCGACAAAG ACTCAAAGTC GAATGAACTT 
TCCGGCGGCA AAAAGAGCAG AGGAAGAGAT AAAGGATCGA AAAAAGACAG GAGCAGTAAA 
AAGAGTGGAA GAAGAGATTG GAAGATGTCT AAATGCAGAA GCGGGAGACG ACAACAGGTT 
ATTGAGAAAC TGGATGAGAA TGAGAGTCTG GAATATGAGT TGACGACCTC TGCTGAATCT 
TTTACCATCC AGTTCTGTCG AAAGAGAGAA AAAGACTGCC TTGAGATTGT TCTGAGCTCA 
TCTGGTGAAT CATCCATTAA GCTTGATGGA CAGGATTTGG GCGATCCCAT TTCGGACTTC 
TTTTACAAGA AAGGGGAGAA GTTCTCCTTC ACTATCACCT ATACATCCCA GCGTGTGGTT 
GTGAGGAACA GCAAATCAGG ATTAGAACTT GTTGCATCGG TCGATGGTGA TGAACTTAAG 
GGTTTTAGGT ACTGCTATGG CACTGCAAAC GGAGGAGAAG GAGATTCTAC ATTCCAGATA 
AGGGAACGTG AATTTTCCGA ATCAGAGATC TCATCATTTG AAAAGCGGTA TAGGGGCAAG 
GGAGGTAGAA AGAGGGGTGG AAAGAGAGAA AGGGGAAACG CCCAGTCACC TCAAGAAAGG 
AAGCGTGGCG GTAGGAAGAA AGGCGACAAA GACTCAAAGT CGAATGAACT TTCCGGCGGC 
AAAAAGAGCA GAGGAAGAGA TTGGAAGATG TCTAAATGCA GAAGCGGGAG ACGACAACAG 
GTTATTGAGA AACTGGATGA GAATGAGAGT CTGGAATATG AGTTGACGAC CTCTGCTGAA 
TCTTTTACCA TCCAGTTCTG TCGAAAGAGA GAAAAAGACT GCCTTGAGAT TGTTCTGAGC 
TCATCTGGTG AATCATCCAT TAAGCTTGAT GGACAGGATT TGGGCGATCC CATTTCGGAC 
TTCTTTTACA AGAAAGGGGA GAAGTTCTCC TTCACTATCA CCTATACATC CCAGCGTGTG 
GTTGTGAGGA ACAGCAAATC AGGATTAGAA CTTGTTGCAT CGGTCGATGG TGATGAACTT 
AAGGGTTTTA GGTACTGCTA TGGCACTGCA AACGGAGGAG AAGGAGATTC TACATTCCAG 
ATAAGGGAAC GTGAATTTTC CGAATCAGAG ATCTCATCAT TTGAAAAGCG GTATAGGGGC 
AAGGGAGGTA GAAAGAGGGG TGGAAAGAGA GAAAGGGGAA ACACCCAGTC ACCCCAAGAA 
AGGAAGCGTG GCGGTAGGAA GAAAGGCGAC AAAGACTCAA AGTCGAATGA ACTTTCCGGC 
GGCAAAAAGA GCAGAGGAAG AGATAAAGGA TCGAAAAAAG ACAGGAGCAG TAAAAAGAGT 
GGAAGAAGAG ATTGGAAGAT GTCTAAATGC AGAAGCGGGA GACGACAACA GGTTATTGAG 
AAACTGGATG AGAATGAGAG TCTGGAATAT GAGTTGACGA CCTCTGCTGA ATCTTTTACC 
ATCCAGTTCT GTCGAAAGAG AGAAAAAGAC TGCCTTGAGA TTGTTCTGAG CTCATCTGGT 
GAATCATCCA TTAAGCTTGA TGGACAGGAT TTGGGCGATC CCATTTCGGA CTTCTTTTAC 
AAGAAAGGGG AGAAGTTCTC CTTCACTATC ACCTATACAT CCCAGCGTGT GGTTGTGAGG 
AACAGCAAAT CAGGATTAGA ACTTGTTGCA TCGGTCGATG GTGATGAACT TAAGGGTTTC 
AGGTACTGCT ATGGCACTGC AAACGGAGGA GAAGGAGATT CTACATTCCA GATAAGGGAA 
CGTGAATTTT CCGAATCAGA GATCTCATCA TTTGAAAAGC GGTATAGGGG CAAGGGAGGT 
AGAAAGAGGG GTGGAAAGAG AGAAAGGGGA AACACCCAGT CACCCCAAGA AAGGAAGCGT 
GGCGGTAGGA AGAAAGGCGA CAAAGACTCA AAGTCGAATG AACTTTCCGG CGGCAAAAAG 
AGCAGAGGAA GAGGGGGTGA AAAGAGAGAA AGGGGAAACA CCGAGTCACC CCAAGAAAGG 
AAGCGTGGTG ATCGTAAAAA AGGCGACAAA GACTCAAAGT CGAATGAAAA GAACAGAGGA 
GGACGACGTG GAGGAAGCAA GAAAGACTCT AAGAGCAGAA AGGGCGGACG AAATCGAGGT 
CGTGGAAGAT GGGCACACCG GAGAGGCAAA TACTATCGAT GGCGTACCGT CAAGGGTGCA 
GTGAAGAGGA CACGCGCTGT GAGCTTTGAC GAACGTCGGA GCGCCATGTC TGTTTTTATC 
AAGACCAAAG CAACTCGATT CGTCTTAGAA TTCTGCCTGT CGTCAGGAGG CAAAGACTGC 
TATACTGCTT CCCTGAGTTC ACAGCGTGAT TCGGCCTCCT TCTTCGAGTA CGGCGGCCAA 
AGAACGGGCG ATAGTCAAGA TGGAGCATTC ATTCGATTTA AACGAAGAAC CAGATTATAT 
GTATCAGTCT CACGTACTAA ACTGACGATC TACAATGACA AGGGAGACGA TCTCTCAGTC 
GCTTTACCCG AGTCATCAGT CATCCAAGAT GTCTACATGT CCACATCAGA TGGGGACTGG 
ACGAAGGTTA GATTCCGCCT ACGAAGATCT GGTAGAACCC TTTACAGCTC CACTGGTCCT 
CTTGCTCGCG GTGCTGCCTT CGAAGATATT GGTTCTAAAG CAACGTCATT CTCCTGTCTA 
ATACGCACCG TGGCCAGCGC CGTCACTTTC GAAATTTGCT TCGATTCTCA GGGAAATGAT 
TGTTACACAA TCGAACTGAG CACGCAACCC AAACAGACAT CCCGAATCCT GTATGGAGGA 
AAATTAGTGA GCAACGAAGA AATTGGTATT TTTATTACCC CTAGCAAGGA GTCGAAAATC 
TTCTTTCGCA TCGAGAAACG GCAGCTGGAG ATCTGGAATA AGGAACCAAA CAATGAACGC 
CAACTCATCG CTAAAATCCG CGGAAAAGGT CGCAGTTTTA ATTCCATTTG GGCCTCTACT 
AGTGATGGTG AATTCACCGA GCTTCAGATG GTCGAGACTG AAGCCGTTAG AGAGTTTTCA 
TCCACGACTA CTCTTGAGAG AACATCGATT GCCACTCAAC TTCAATTCCC AGGGCAACTG 
GTGTTAAGCT TTGCTCTCGA GGCTGACAAG GTCTTCATGG AGTTTCGTCG CCCAGATGGG 
AAGAGTTATA CGATAGAAAT GGGTACGAAA GCAGGTCAGC CATCTGTTAT TCGCAGTGGG 
GGTAGTAACC TAGTAGATCC ACAGGACCAA CCATTCCTCG AGTTTGGCAA TGACATTGAC 
CCACCTAACC AACTCGTCAT CGATGTGTTC GAGTCTAGCG TGACTGTTTC TACTGCCCGT 
GGTCAAAAGA TGGTGGCACC TGTTGCAGAA GCTTATCTCT ACAACCATTT CGACATTTGG 
ACGGTGGGTG GTGGTATTGC TCTCGCTGTG GAAGAAATCG AAGATGAAGA TTCGAAAGAT 
TCTCTTGCAA GTAAGACAGA GATTGAAGAA ACAGAACAGG AAATAGAATC AGTAGAAGAG 
GAACTTAAGG GTAACAACCC CGGCGTTCAA CCAGTGCGAA AACGAAAAGG AAAAACAAGC 
GGCAAAAGAC GAGGAAAAAC AGGCGGCAGA AGACGCGGAA AAGCTAAAGA GTCAAAATCA 
GATGACGACA CAGAACCAGA AAACGAACCA GTAGAAGAGG CCAAGGGTGA CAGCCCCAGC 
GTTCAACAAG AGCGAAAACG AAATGGAAAA AAAGGCGGCA AAAGACGAGG AAAAACAGGC 
GACAGAAGAC GCGGAAAAGC TAAAGAGTCA AAATCAGATG ACGACACAGA ACCAGAAAAC 
GAACCAGTAG AAGAGGCCAA GGGTGACAGC CCCAGCGTTC AACAAGAGCG AAAACGAAAT 
GGAAAAAAAG GCGGCAAAAG ACGAGGAAAA ACAGGCGACA GAAGACGCGG AAAAGCTAAA 
GAGTCATCAG ATGACGACAC AGAACCAGAA AACGAACCAG TAGAAGAGGC CAAGGGTGAC 
AGCCCCGGCA TTCAACAAGA GCGAAAACGA AAAGGAAAAA TAGGCGGCAA GAGACGAGGA 
AAAACAGGCG GCAGAATACG CGGAAAAGCT AAAGAGTCAC AATCAGATGA CGACACAGAA 
CCAGAAAACG AACCAGTAGA AGAGGCCAAG GGTGACAGCC CCAGCGTTCA ACAAGAGCGA 
AAACGAAATG GAAAAAAAGG CGGCAAAAGA CGAGGAAAAA CAGGCGACAG AAGACGCGGA 
AAAGCTAAAG AGTCATCAGA TGACGACACA GAACCTGTTA TTGAACCAGT AATACAGGAC 
ACATTAGAAG AAACGAAACC TGTGAACAGT GAAGTATTCG AAGAATTGAA GGGTAACAAC 
CCTGGCGTTG AAGTAAAGAG AACTAGAGGT GACAGAATCA TAGACAATCA AGATATTGAA 
GACACAACAC AAGAGTCATC TCTGACACCA ACTCAAGTTC CACCAACAAT TGAGACAATA 
CGAAACACGG GATCAACCGA TGAGCCAGAG GTCAAAGAGA AAGTTAGCCA GGACGTAGTA 
GAGCCGGTCG TGAAACCGTT CAACGAAGAA CCTGATAAGT CAGCCACTCT TGAGACCAAC 
GTACTTGGCG TCGCTGCGTT CTTCGATCAA ACCTTGGATA TAGATCGTTT ATTACAGCTT 
CAGTTTGATT TCAGTGTCAC CATTAACACC GGTTTCGCTA TCCTCTTGAC ACAAGGACAA 
ATTCTTAGGG GAGATTATTA CTCAATCGGT ATAACCGAGG GAAACGTCTA CATGGTCCAG 
CGATGTAATA AGGAAACAGC GGAATGTAAT CGACTCTTCA GCAGGAAAAT TGATCCACTT 
GGTGAAGGAA CAGGAATATT CACGCTGAAG ATTCAGAAGA TCCTAAATGG CAATGTAGAC 
CCTGATGCTG ATGGAGCATA CTCCTACTGG TTTTCATTGC TGTATGAAGG AGCTGTGGTC 
GGAGAACAGG TGGAAGTACA GTATCCAAAT ACATTCTATA TTAGGTACAT TGGATGCATC 
TCCTACAAGT ACGTGGCCAC GTGGAATCTC TTCTCTGTCG TCTCACCGGG TTCTACCCAG 
AGATCCTCTG CAATCATACT CCGGAACGCC GAAGAAACAA GTAGTCTGGA GGTGAATTCA 
TTTGATCGCG AAGTTGTATT CAGTGTCAAA GCCCAAGATT TTTTGATAAT TCGTCTTTAC 
TCCGTTGGCG ACGTCGTCTA CACAATCCTC GTTGGAGACG AAGGCAATAA CTATGTTACG 
GGATCGTCCG GTACTGGTGA TAAATCTCCG GATGACGCGT CGCGTCGCGT TGAAGGAGGT 
GTGTTAAGCT TGGCGGGCAA GCAGTACTAC CTTTCTTGGC GAAACGGCAG GATGAAGTTC 
GGCGAAACGG AGGAGGACAG CGAGGCGATG ATGAATTGGG AGATTGGGGA AATCTCGGTG 
ACTAAGCTAG AATTCATTGC TGCTTACGAC AGCTTGTATC TCATACACAC ATACAAGTAC 
TTCTACGTAT TTGGCGATGT TTTGCAGGCG ACTCATGATC GAATACGGGC AACTTCTCTC 
ACTTTCTTCA CTCTCCGTCG CGAGCGTGGC GTGCCATACA TCCACCTCAA GATCAAGGCC 
TCTGGGACTG TCTTGATTGC GCTCGCCAAA GACCAGGAGC TTTCTGAATC CGACATGGTT 
TTGATCAAGA TGAGTTCCCG GAGGATAACT CTCTCCACAT GCCTTGGTTG CGAAACAATC 
TACTCGGTTC CGATCCCTAA AGACCAAGCG GCCCTTCTAG ATCCGTCCTC ATTCCTTGAG 
TACCAGATCA CGTGTGAAGG CAACAGTCTG AAGATCTTCT TAACCGGTCA GAAGGAACCC 
TTGCTGGACT ATGGTGGCAT TGATCGAGAC GCCCACTATG GCTATGATGG CTTCGGAGGA 
ACTATCGGTA GCAATTATCA CATCAAGCTC AACCAAGGAA CGATCTACAG AGAGGAGCAT 
TGTCTTGACG TCAACACCTT CTTGGGTGGA GGAACGAAGC TTCTGAAGAT CGTCCACTCT 
GACCAGATGA TTGCTTTATT TGATGCTGAG AAACAGCTCG AGGCAGAGCT TGCCATGGAA 
GATACGAAGA CGGAGCTGCG TACTACCCGT GGTGGCGAGG CCGTTCAGAT AAGCCAAAGT 
AGTAGTTGCA TCTTCCACGC TCCCTACACT CTAGTCTGGA TGGACTTCAG CGGTGGGGCT 
ATTCGGTATG GTACCTACGA CGAGGCGAAT CGCCTGAGAA GTGACCCGTG TATCACCTAC 
GACCTCGGTG ATGATCAGGA GGTCAAATAC ATGTCGTTTG GTCAACAGCA TTCCTCGTGG 
GATGTTACAC ATCACGTGAT TGAGAAGAGG ATTGTGTACA AGGAGTTGGT CTCCCATGTA 
CAAGAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030830440.1
CDS	133..7644
Translation	MNTMNALAFGAVLLLAAVASTSGGGSTSQVADNKERFSYSGGGGLTGLNVKQGGFTQVSHSTKEKTVTTEKETRKSKSKKRKGGKVSTQETSKKETNGGKVKNQRGNTQETSRKRKGRGKNKGNEKTEVKQREGNRGRDGDGKGDRKGRKGSTETVINKERSGGRRRSKNKSGKGSTKGSSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQSVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNAQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDKGSKKDRSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNAQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNTQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDKGSKKDRSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNTQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRGGEKRERGNTESPQERKRGDRKKGDKDSKSNEKNRGGRRGGSKKDSKSRKGGRNRGRGRWAHRRGKYYRWRTVKGAVKRTRAVSFDERRSAMSVFIKTKATRFVLEFCLSSGGKDCYTASLSSQRDSASFFEYGGQRTGDSQDGAFIRFKRRTRLYVSVSRTKLTIYNDKGDDLSVALPESSVIQDVYMSTSDGDWTKVRFRLRRSGRTLYSSTGPLARGAAFEDIGSKATSFSCLIRTVASAVTFEICFDSQGNDCYTIELSTQPKQTSRILYGGKLVSNEEIGIFITPSKESKIFFRIEKRQLEIWNKEPNNERQLIAKIRGKGRSFNSIWASTSDGEFTELQMVETEAVREFSSTTTLERTSIATQLQFPGQLVLSFALEADKVFMEFRRPDGKSYTIEMGTKAGQPSVIRSGGSNLVDPQDQPFLEFGNDIDPPNQLVIDVFESSVTVSTARGQKMVAPVAEAYLYNHFDIWTVGGGIALAVEEIEDEDSKDSLASKTEIEETEQEIESVEEELKGNNPGVQPVRKRKGKTSGKRRGKTGGRRRGKAKESKSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESKSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESSDDDTEPENEPVEEAKGDSPGIQQERKRKGKIGGKRRGKTGGRIRGKAKESQSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESSDDDTEPVIEPVIQDTLEETKPVNSEVFEELKGNNPGVEVKRTRGDRIIDNQDIEDTTQESSLTPTQVPPTIETIRNTGSTDEPEVKEKVSQDVVEPVVKPFNEEPDKSATLETNVLGVAAFFDQTLDIDRLLQLQFDFSVTINTGFAILLTQGQILRGDYYSIGITEGNVYMVQRCNKETAECNRLFSRKIDPLGEGTGIFTLKIQKILNGNVDPDADGAYSYWFSLLYEGAVVGEQVEVQYPNTFYIRYIGCISYKYVATWNLFSVVSPGSTQRSSAIILRNAEETSSLEVNSFDREVVFSVKAQDFLIIRLYSVGDVVYTILVGDEGNNYVTGSSGTGDKSPDDASRRVEGGVLSLAGKQYYLSWRNGRMKFGETEEDSEAMMNWEIGEISVTKLEFIAAYDSLYLIHTYKYFYVFGDVLQATHDRIRATSLTFFTLRRERGVPYIHLKIKASGTVLIALAKDQELSESDMVLIKMSSRRITLSTCLGCETIYSVPIPKDQAALLDPSSFLEYQITCEGNSLKIFLTGQKEPLLDYGGIDRDAHYGYDGFGGTIGSNYHIKLNQGTIYREEHCLDVNTFLGGGTKLLKIVHSDQMIALFDAEKQLEAELAMEDTKTELRTTRGGEAVQISQSSSCIFHAPYTLVWMDFSGGAIRYGTYDEANRLRSDPCITYDLGDDQEVKYMSFGQQHSSWDVTHHVIEKRIVYKELVSHVQEP

Target ORF information:

RefSeq Version	XM_030974580.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030974580.1

ORF Insert Sequence:

ATGAACACAA TGAACGCCCT GGCGTTTGGC GCTGTTCTGC TGTTAGCTGC AGTCGCGTCG 
ACCTCAGGCG GCGGTAGCAC CAGTCAAGTT GCTGATAATA AAGAGCGATT CTCATACAGC 
GGCGGAGGTG GTCTCACAGG CCTGAACGTC AAGCAAGGTG GTTTTACTCA GGTTTCCCAC 
TCGACAAAAG AAAAGACTGT CACCACGGAG AAAGAAACTA GGAAGAGCAA ATCAAAAAAG 
CGGAAAGGCG GTAAGGTGTC TACTCAAGAG ACTTCAAAGA AAGAAACTAA CGGGGGCAAA 
GTGAAGAACC AGAGAGGCAA TACGCAAGAG ACTTCACGAA AACGAAAAGG AAGGGGAAAG 
AATAAAGGAA ACGAAAAAAC TGAAGTCAAA CAACGAGAAG GAAATCGCGG TCGAGATGGA 
GATGGCAAAG GAGATCGTAA AGGAAGAAAG GGAAGTACTG AGACGGTGAT CAATAAAGAA 
AGGAGCGGTG GTCGGCGTCG AAGTAAAAAC AAGAGTGGTA AGGGATCGAC CAAAGGATCG 
AGCAGTAAAA AGAGTGGAAG AAGAGATTGG AAGATGTCTA AATGCAGAAG CGGGAGACGA 
CAACAGGTTA TTGAGAAACT GGATGAGAAT GAGAGTCTGG AATATGAGTT GACGACCTCT 
GCTGAATCTT TTACCATCCA GTTCTGTCGA AAGAGAGAAA AAGACTGCCT TGAGATTGTT 
CTGAGCTCAT CTGGTGAATC ATCCATTAAG CTTGATGGAC AGGATTTGGG CGATCCCATT 
TCGGACTTCT TTTACAAGAA AGGGGAGAAG TTCTCCTTCA CTATCACCTA TACATCCCAG 
AGTGTGGTTG TGAGGAACAG CAAATCAGGA TTAGAACTTG TTGCATCGGT CGATGGTGAT 
GAACTTAAGG GTTTCAGGTA CTGCTATGGC ACTGCAAACG GAGGAGAAGG AGATTCTACA 
TTCCAGATAA GGGAACGTGA ATTTTCCGAA TCAGAGATCT CATCATTTGA AAAGCGGTAT 
AGGGGCAAGG GAGGTAGAAA GAGGGGTGGA AAGAGAGAAA GGGGAAACGC CCAGTCACCT 
CAAGAAAGGA AGCGTGGCGG TAGGAAGAAA GGCGACAAAG ACTCAAAGTC GAATGAACTT 
TCCGGCGGCA AAAAGAGCAG AGGAAGAGAT AAAGGATCGA AAAAAGACAG GAGCAGTAAA 
AAGAGTGGAA GAAGAGATTG GAAGATGTCT AAATGCAGAA GCGGGAGACG ACAACAGGTT 
ATTGAGAAAC TGGATGAGAA TGAGAGTCTG GAATATGAGT TGACGACCTC TGCTGAATCT 
TTTACCATCC AGTTCTGTCG AAAGAGAGAA AAAGACTGCC TTGAGATTGT TCTGAGCTCA 
TCTGGTGAAT CATCCATTAA GCTTGATGGA CAGGATTTGG GCGATCCCAT TTCGGACTTC 
TTTTACAAGA AAGGGGAGAA GTTCTCCTTC ACTATCACCT ATACATCCCA GCGTGTGGTT 
GTGAGGAACA GCAAATCAGG ATTAGAACTT GTTGCATCGG TCGATGGTGA TGAACTTAAG 
GGTTTTAGGT ACTGCTATGG CACTGCAAAC GGAGGAGAAG GAGATTCTAC ATTCCAGATA 
AGGGAACGTG AATTTTCCGA ATCAGAGATC TCATCATTTG AAAAGCGGTA TAGGGGCAAG 
GGAGGTAGAA AGAGGGGTGG AAAGAGAGAA AGGGGAAACG CCCAGTCACC TCAAGAAAGG 
AAGCGTGGCG GTAGGAAGAA AGGCGACAAA GACTCAAAGT CGAATGAACT TTCCGGCGGC 
AAAAAGAGCA GAGGAAGAGA TTGGAAGATG TCTAAATGCA GAAGCGGGAG ACGACAACAG 
GTTATTGAGA AACTGGATGA GAATGAGAGT CTGGAATATG AGTTGACGAC CTCTGCTGAA 
TCTTTTACCA TCCAGTTCTG TCGAAAGAGA GAAAAAGACT GCCTTGAGAT TGTTCTGAGC 
TCATCTGGTG AATCATCCAT TAAGCTTGAT GGACAGGATT TGGGCGATCC CATTTCGGAC 
TTCTTTTACA AGAAAGGGGA GAAGTTCTCC TTCACTATCA CCTATACATC CCAGCGTGTG 
GTTGTGAGGA ACAGCAAATC AGGATTAGAA CTTGTTGCAT CGGTCGATGG TGATGAACTT 
AAGGGTTTTA GGTACTGCTA TGGCACTGCA AACGGAGGAG AAGGAGATTC TACATTCCAG 
ATAAGGGAAC GTGAATTTTC CGAATCAGAG ATCTCATCAT TTGAAAAGCG GTATAGGGGC 
AAGGGAGGTA GAAAGAGGGG TGGAAAGAGA GAAAGGGGAA ACACCCAGTC ACCCCAAGAA 
AGGAAGCGTG GCGGTAGGAA GAAAGGCGAC AAAGACTCAA AGTCGAATGA ACTTTCCGGC 
GGCAAAAAGA GCAGAGGAAG AGATAAAGGA TCGAAAAAAG ACAGGAGCAG TAAAAAGAGT 
GGAAGAAGAG ATTGGAAGAT GTCTAAATGC AGAAGCGGGA GACGACAACA GGTTATTGAG 
AAACTGGATG AGAATGAGAG TCTGGAATAT GAGTTGACGA CCTCTGCTGA ATCTTTTACC 
ATCCAGTTCT GTCGAAAGAG AGAAAAAGAC TGCCTTGAGA TTGTTCTGAG CTCATCTGGT 
GAATCATCCA TTAAGCTTGA TGGACAGGAT TTGGGCGATC CCATTTCGGA CTTCTTTTAC 
AAGAAAGGGG AGAAGTTCTC CTTCACTATC ACCTATACAT CCCAGCGTGT GGTTGTGAGG 
AACAGCAAAT CAGGATTAGA ACTTGTTGCA TCGGTCGATG GTGATGAACT TAAGGGTTTC 
AGGTACTGCT ATGGCACTGC AAACGGAGGA GAAGGAGATT CTACATTCCA GATAAGGGAA 
CGTGAATTTT CCGAATCAGA GATCTCATCA TTTGAAAAGC GGTATAGGGG CAAGGGAGGT 
AGAAAGAGGG GTGGAAAGAG AGAAAGGGGA AACACCCAGT CACCCCAAGA AAGGAAGCGT 
GGCGGTAGGA AGAAAGGCGA CAAAGACTCA AAGTCGAATG AACTTTCCGG CGGCAAAAAG 
AGCAGAGGAA GAGGGGGTGA AAAGAGAGAA AGGGGAAACA CCGAGTCACC CCAAGAAAGG 
AAGCGTGGTG ATCGTAAAAA AGGCGACAAA GACTCAAAGT CGAATGAAAA GAACAGAGGA 
GGACGACGTG GAGGAAGCAA GAAAGACTCT AAGAGCAGAA AGGGCGGACG AAATCGAGGT 
CGTGGAAGAT GGGCACACCG GAGAGGCAAA TACTATCGAT GGCGTACCGT CAAGGGTGCA 
GTGAAGAGGA CACGCGCTGT GAGCTTTGAC GAACGTCGGA GCGCCATGTC TGTTTTTATC 
AAGACCAAAG CAACTCGATT CGTCTTAGAA TTCTGCCTGT CGTCAGGAGG CAAAGACTGC 
TATACTGCTT CCCTGAGTTC ACAGCGTGAT TCGGCCTCCT TCTTCGAGTA CGGCGGCCAA 
AGAACGGGCG ATAGTCAAGA TGGAGCATTC ATTCGATTTA AACGAAGAAC CAGATTATAT 
GTATCAGTCT CACGTACTAA ACTGACGATC TACAATGACA AGGGAGACGA TCTCTCAGTC 
GCTTTACCCG AGTCATCAGT CATCCAAGAT GTCTACATGT CCACATCAGA TGGGGACTGG 
ACGAAGGTTA GATTCCGCCT ACGAAGATCT GGTAGAACCC TTTACAGCTC CACTGGTCCT 
CTTGCTCGCG GTGCTGCCTT CGAAGATATT GGTTCTAAAG CAACGTCATT CTCCTGTCTA 
ATACGCACCG TGGCCAGCGC CGTCACTTTC GAAATTTGCT TCGATTCTCA GGGAAATGAT 
TGTTACACAA TCGAACTGAG CACGCAACCC AAACAGACAT CCCGAATCCT GTATGGAGGA 
AAATTAGTGA GCAACGAAGA AATTGGTATT TTTATTACCC CTAGCAAGGA GTCGAAAATC 
TTCTTTCGCA TCGAGAAACG GCAGCTGGAG ATCTGGAATA AGGAACCAAA CAATGAACGC 
CAACTCATCG CTAAAATCCG CGGAAAAGGT CGCAGTTTTA ATTCCATTTG GGCCTCTACT 
AGTGATGGTG AATTCACCGA GCTTCAGATG GTCGAGACTG AAGCCGTTAG AGAGTTTTCA 
TCCACGACTA CTCTTGAGAG AACATCGATT GCCACTCAAC TTCAATTCCC AGGGCAACTG 
GTGTTAAGCT TTGCTCTCGA GGCTGACAAG GTCTTCATGG AGTTTCGTCG CCCAGATGGG 
AAGAGTTATA CGATAGAAAT GGGTACGAAA GCAGGTCAGC CATCTGTTAT TCGCAGTGGG 
GGTAGTAACC TAGTAGATCC ACAGGACCAA CCATTCCTCG AGTTTGGCAA TGACATTGAC 
CCACCTAACC AACTCGTCAT CGATGTGTTC GAGTCTAGCG TGACTGTTTC TACTGCCCGT 
GGTCAAAAGA TGGTGGCACC TGTTGCAGAA GCTTATCTCT ACAACCATTT CGACATTTGG 
ACGGTGGGTG GTGGTATTGC TCTCGCTGTG GAAGAAATCG AAGATGAAGA TTCGAAAGAT 
TCTCTTGCAA GTAAGACAGA GATTGAAGAA ACAGAACAGG AAATAGAATC AGTAGAAGAG 
GAACTTAAGG GTAACAACCC CGGCGTTCAA CCAGTGCGAA AACGAAAAGG AAAAACAAGC 
GGCAAAAGAC GAGGAAAAAC AGGCGGCAGA AGACGCGGAA AAGCTAAAGA GTCAAAATCA 
GATGACGACA CAGAACCAGA AAACGAACCA GTAGAAGAGG CCAAGGGTGA CAGCCCCAGC 
GTTCAACAAG AGCGAAAACG AAATGGAAAA AAAGGCGGCA AAAGACGAGG AAAAACAGGC 
GACAGAAGAC GCGGAAAAGC TAAAGAGTCA AAATCAGATG ACGACACAGA ACCAGAAAAC 
GAACCAGTAG AAGAGGCCAA GGGTGACAGC CCCAGCGTTC AACAAGAGCG AAAACGAAAT 
GGAAAAAAAG GCGGCAAAAG ACGAGGAAAA ACAGGCGACA GAAGACGCGG AAAAGCTAAA 
GAGTCATCAG ATGACGACAC AGAACCAGAA AACGAACCAG TAGAAGAGGC CAAGGGTGAC 
AGCCCCGGCA TTCAACAAGA GCGAAAACGA AAAGGAAAAA TAGGCGGCAA GAGACGAGGA 
AAAACAGGCG GCAGAATACG CGGAAAAGCT AAAGAGTCAC AATCAGATGA CGACACAGAA 
CCAGAAAACG AACCAGTAGA AGAGGCCAAG GGTGACAGCC CCAGCGTTCA ACAAGAGCGA 
AAACGAAATG GAAAAAAAGG CGGCAAAAGA CGAGGAAAAA CAGGCGACAG AAGACGCGGA 
AAAGCTAAAG AGTCATCAGA TGACGACACA GAACCTGTTA TTGAACCAGT AATACAGGAC 
ACATTAGAAG AAACGAAACC TGTGAACAGT GAAGTATTCG AAGAATTGAA GGGTAACAAC 
CCTGGCGTTG AAGTAAAGAG AACTAGAGGT GACAGAATCA TAGACAATCA AGATATTGAA 
GACACAACAC AAGAGTCATC TCTGACACCA ACTCAAGTTC CACCAACAAT TGAGACAATA 
CGAAACACGG GATCAACCGA TGAGCCAGAG GTCAAAGAGA AAGTTAGCCA GGACGTAGTA 
GAGCCGGTCG TGAAACCGTT CAACGAAGAA CCTGATAAGT CAGCCACTCT TGAGACCAAC 
GTACTTGGCG TCGCTGCGTT CTTCGATCAA ACCTTGGATA TAGATCGTTT ATTACAGCTT 
CAGTTTGATT TCAGTGTCAC CATTAACACC GGTTTCGCTA TCCTCTTGAC ACAAGGACAA 
ATTCTTAGGG GAGATTATTA CTCAATCGGT ATAACCGAGG GAAACGTCTA CATGGTCCAG 
CGATGTAATA AGGAAACAGC GGAATGTAAT CGACTCTTCA GCAGGAAAAT TGATCCACTT 
GGTGAAGGAA CAGGAATATT CACGCTGAAG ATTCAGAAGA TCCTAAATGG CAATGTAGAC 
CCTGATGCTG ATGGAGCATA CTCCTACTGG TTTTCATTGC TGTATGAAGG AGCTGTGGTC 
GGAGAACAGG TGGAAGTACA GTATCCAAAT ACATTCTATA TTAGGTACAT TGGATGCATC 
TCCTACAAGT ACGTGGCCAC GTGGAATCTC TTCTCTGTCG TCTCACCGGG TTCTACCCAG 
AGATCCTCTG CAATCATACT CCGGAACGCC GAAGAAACAA GTAGTCTGGA GGTGAATTCA 
TTTGATCGCG AAGTTGTATT CAGTGTCAAA GCCCAAGATT TTTTGATAAT TCGTCTTTAC 
TCCGTTGGCG ACGTCGTCTA CACAATCCTC GTTGGAGACG AAGGCAATAA CTATGTTACG 
GGATCGTCCG GTACTGGTGA TAAATCTCCG GATGACGCGT CGCGTCGCGT TGAAGGAGGT 
GTGTTAAGCT TGGCGGGCAA GCAGTACTAC CTTTCTTGGC GAAACGGCAG GATGAAGTTC 
GGCGAAACGG AGGAGGACAG CGAGGCGATG ATGAATTGGG AGATTGGGGA AATCTCGGTG 
ACTAAGCTAG AATTCATTGC TGCTTACGAC AGCTTGTATC TCATACACAC ATACAAGTAC 
TTCTACGTAT TTGGCGATGT TTTGCAGGCG ACTCATGATC GAATACGGGC AACTTCTCTC 
ACTTTCTTCA CTCTCCGTCG CGAGCGTGGC GTGCCATACA TCCACCTCAA GATCAAGGCC 
TCTGGGACTG TCTTGATTGC GCTCGCCAAA GACCAGGAGC TTTCTGAATC CGACATGGTT 
TTGATCAAGA TGAGTTCCCG GAGGATAACT CTCTCCACAT GCCTTGGTTG CGAAACAATC 
TACTCGGTTC CGATCCCTAA AGACCAAGCG GCCCTTCTAG ATCCGTCCTC ATTCCTTGAG 
TACCAGATCA CGTGTGAAGG CAACAGTCTG AAGATCTTCT TAACCGGTCA GAAGGAACCC 
TTGCTGGACT ATGGTGGCAT TGATCGAGAC GCCCACTATG GCTATGATGG CTTCGGAGGA 
ACTATCGGTA GCAATTATCA CATCAAGCTC AACCAAGGAA CGATCTACAG AGAGGAGCAT 
TGTCTTGACG TCAACACCTT CTTGGGTGGA GGAACGAAGC TTCTGAAGAT CGTCCACTCT 
GACCAGATGA TTGCTTTATT TGATGCTGAG AAACAGCTCG AGGCAGAGCT TGCCATGGAA 
GATACGAAGA CGGAGCTGCG TACTACCCGT GGTGGCGAGG CCGTTCAGAT AAGCCAAAGT 
AGTAGTTGCA TCTTCCACGC TCCCTACACT CTAGTCTGGA TGGACTTCAG CGGTGGGGCT 
ATTCGGTATG GTACCTACGA CGAGGCGAAT CGCCTGAGAA GTGACCCGTG TATCACCTAC 
GACCTCGGTG ATGATCAGGA GGTCAAATAC ATGTCGTTTG GTCAACAGCA TTCCTCGTGG 
GATGTTACAC ATCACGTGAT TGAGAAGAGG ATTGTGTACA AGGAGTTGGT CTCCCATGTA 
CAAGAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC576141 ( XM_030974581.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030830441.1 Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X4, mRNA.

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CloneID	OSd69454
Clone ID Related Accession (Same CDS sequence)	XM_030974581.1
Accession Version	XM_030974581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	uncharacterized protein LOC576141 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAA TGAACGCCCT GGCGTTTGGC GCTGTTCTGC TGTTAGCTGC AGTCGCGTCG 
ACCTCAGGCG GCGGTAGCAC CAGTCAAGTT GCTGATAATA AAGAGCGATT CTCATACAGC 
GGCGGAGGTG GTCTCACAGG CCTGAACGTC AAGCAAGGTG GTTTTACTCA GGTTTCCCAC 
TCGACAAAAG AAAAGACTGT CACCACGGAG AAAGAAACTA GGAAGAGCAA ATCAAAAAAG 
CGGAAAGGCG GTAAGGTGTC TACTCAAGAG ACTTCAAAGA AAGAAACTAA CGGGGGCAAA 
GTGAAGAACC AGAGAGGCAA TACGCAAGAG ACTTCACGAA AACGAAAAGG AAGGGGAAAG 
AATAAAGGAA ACGAAAAAAC TGAAGTCAAA CAACGAGAAG GAAATCGCGG TCGAGATGGA 
GATGGCAAAG GAGATCGTAA AGGAAGAAAG GGAAGTACTG AGACGGTGAT CAATAAAGAA 
AGGAGCGGTG GTCGGCGTCG AAGTAAAAAC AAGAGTGGTA AGGGATCGAC CAAAGGATCG 
AGCAGTAAAA AGAGTGGAAG AAGAGATTGG AAGATGTCTA AATGCAGAAG CGGGAGACGA 
CAACAGGTTA TTGAGAAACT GGATGAGAAT GAGAGTCTGG AATATGAGTT GACGACCTCT 
GCTGAATCTT TTACCATCCA GTTCTGTCGA AAGAGAGAAA AAGACTGCCT TGAGATTGTT 
CTGAGCTCAT CTGGTGAATC ATCCATTAAG CTTGATGGAC AGGATTTGGG CGATCCCATT 
TCGGACTTCT TTTACAAGAA AGGGGAGAAG TTCTCCTTCA CTATCACCTA TACATCCCAG 
AGTGTGGTTG TGAGGAACAG CAAATCAGGA TTAGAACTTG TTGCATCGGT CGATGGTGAT 
GAACTTAAGG GTTTCAGGTA CTGCTATGGC ACTGCAAACG GAGGAGAAGG AGATTCTACA 
TTCCAGATAA GGGAACGTGA ATTTTCCGAA TCAGAGATCT CATCATTTGA AAAGCGGTAT 
AGGGGCAAGG GAGGTAGAAA GAGGGGTGGA AAGAGAGAAA GGGGAAACGC CCAGTCACCT 
CAAGAAAGGA AGCGTGGCGG TAGGAAGAAA GGCGACAAAG ACTCAAAGTC GAATGAACTT 
TCCGGCGGCA AAAAGAGCAG AGGAAGAGAT AAAGGATCGA AAAAAGACAG GAGCAGTAAA 
AAGAGTGGAA GAAGAGATTG GAAGATGTCT AAATGCAGAA GCGGGAGACG ACAACAGGTT 
ATTGAGAAAC TGGATGAGAA TGAGAGTCTG GAATATGAGT TGACGACCTC TGCTGAATCT 
TTTACCATCC AGTTCTGTCG AAAGAGAGAA AAAGACTGCC TTGAGATTGT TCTGAGCTCA 
TCTGGTGAAT CATCCATTAA GCTTGATGGA CAGGATTTGG GCGATCCCAT TTCGGACTTC 
TTTTACAAGA AAGGGGAGAA GTTCTCCTTC ACTATCACCT ATACATCCCA GCGTGTGGTT 
GTGAGGAACA GCAAATCAGG ATTAGAACTT GTTGCATCGG TCGATGGTGA TGAACTTAAG 
GGTTTTAGGT ACTGCTATGG CACTGCAAAC GGAGGAGAAG GAGATTCTAC ATTCCAGATA 
AGGGAACGTG AATTTTCCGA ATCAGAGATC TCATCATTTG AAAAGCGGTA TAGGGGCAAG 
GGAGGTAGAA AGAGGGGTGG AAAGAGAGAA AGGGGAAACG CCCAGTCACC TCAAGAAAGG 
AAGCGTGGCG GTAGGAAGAA AGGCGACAAA GACTCAAAGT CGAATGAACT TTCCGGCGGC 
AAAAAGAGCA GAGGAAGAGA TAAAGGATCG AAAAAAGACA GGAGCAGTAA AAAGAGTGGA 
AGAAGAGATT GGAAGATGTC TAAATGCAGA AGCGGGAGAC GACAACAGGT TATTGAGAAA 
CTGGATGAGA ATGAGAGTCT GGAATATGAG TTGACGACCT CTGCTGAATC TTTTACCATC 
CAGTTCTGTC GAAAGAGAGA AAAAGACTGC CTTGAGATTG TTCTGAGCTC ATCTGGTGAA 
TCATCCATTA AGCTTGATGG ACAGGATTTG GGCGATCCCA TTTCGGACTT CTTTTACAAG 
AAAGGGGAGA AGTTCTCCTT CACTATCACC TATACATCCC AGCGTGTGGT TGTGAGGAAC 
AGCAAATCAG GATTAGAACT TGTTGCATCG GTCGATGGTG ATGAACTTAA GGGTTTTAGG 
TACTGCTATG GCACTGCAAA CGGAGGAGAA GGAGATTCTA CATTCCAGAT AAGGGAACGT 
GAATTTTCCG AATCAGAGAT CTCATCATTT GAAAAGCGGT ATAGGGGCAA GGGAGGTAGA 
AAGAGGGGTG GAAAGAGAGA AAGGGGAAAC ACCCAGTCAC CCCAAGAAAG GAAGCGTGGC 
GGTAGGAAGA AAGGCGACAA AGACTCAAAG TCGAATGAAC TTTCCGGCGG CAAAAAGAGC 
AGAGGAAGAG ATTGGAAGAT GTCTAAATGC AGAAGCGGGA GACGACAACA GGTTATTGAG 
AAACTGGATG AGAATGAGAG TCTGGAATAT GAGTTGACGA CCTCTGCTGA ATCTTTTACC 
ATCCAGTTCT GTCGAAAGAG AGAAAAAGAC TGCCTTGAGA TTGTTCTGAG CTCATCTGGT 
GAATCATCCA TTAAGCTTGA TGGACAGGAT TTGGGCGATC CCATTTCGGA CTTCTTTTAC 
AAGAAAGGGG AGAAGTTCTC CTTCACTATC ACCTATACAT CCCAGCGTGT GGTTGTGAGG 
AACAGCAAAT CAGGATTAGA ACTTGTTGCA TCGGTCGATG GTGATGAACT TAAGGGTTTC 
AGGTACTGCT ATGGCACTGC AAACGGAGGA GAAGGAGATT CTACATTCCA GATAAGGGAA 
CGTGAATTTT CCGAATCAGA GATCTCATCA TTTGAAAAGC GGTATAGGGG CAAGGGAGGT 
AGAAAGAGGG GTGGAAAGAG AGAAAGGGGA AACACCCAGT CACCCCAAGA AAGGAAGCGT 
GGCGGTAGGA AGAAAGGCGA CAAAGACTCA AAGTCGAATG AACTTTCCGG CGGCAAAAAG 
AGCAGAGGAA GAGGGGGTGA AAAGAGAGAA AGGGGAAACA CCGAGTCACC CCAAGAAAGG 
AAGCGTGGTG ATCGTAAAAA AGGCGACAAA GACTCAAAGT CGAATGAAAA GAACAGAGGA 
GGACGACGTG GAGGAAGCAA GAAAGACTCT AAGAGCAGAA AGGGCGGACG AAATCGAGGT 
CGTGGAAGAT GGGCACACCG GAGAGGCAAA TACTATCGAT GGCGTACCGT CAAGGGTGCA 
GTGAAGAGGA CACGCGCTGT GAGCTTTGAC GAACGTCGGA GCGCCATGTC TGTTTTTATC 
AAGACCAAAG CAACTCGATT CGTCTTAGAA TTCTGCCTGT CGTCAGGAGG CAAAGACTGC 
TATACTGCTT CCCTGAGTTC ACAGCGTGAT TCGGCCTCCT TCTTCGAGTA CGGCGGCCAA 
AGAACGGGCG ATAGTCAAGA TGGAGCATTC ATTCGATTTA AACGAAGAAC CAGATTATAT 
GTATCAGTCT CACGTACTAA ACTGACGATC TACAATGACA AGGGAGACGA TCTCTCAGTC 
GCTTTACCCG AGTCATCAGT CATCCAAGAT GTCTACATGT CCACATCAGA TGGGGACTGG 
ACGAAGGTTA GATTCCGCCT ACGAAGATCT GGTAGAACCC TTTACAGCTC CACTGGTCCT 
CTTGCTCGCG GTGCTGCCTT CGAAGATATT GGTTCTAAAG CAACGTCATT CTCCTGTCTA 
ATACGCACCG TGGCCAGCGC CGTCACTTTC GAAATTTGCT TCGATTCTCA GGGAAATGAT 
TGTTACACAA TCGAACTGAG CACGCAACCC AAACAGACAT CCCGAATCCT GTATGGAGGA 
AAATTAGTGA GCAACGAAGA AATTGGTATT TTTATTACCC CTAGCAAGGA GTCGAAAATC 
TTCTTTCGCA TCGAGAAACG GCAGCTGGAG ATCTGGAATA AGGAACCAAA CAATGAACGC 
CAACTCATCG CTAAAATCCG CGGAAAAGGT CGCAGTTTTA ATTCCATTTG GGCCTCTACT 
AGTGATGGTG AATTCACCGA GCTTCAGATG GTCGAGACTG AAGCCGTTAG AGAGTTTTCA 
TCCACGACTA CTCTTGAGAG AACATCGATT GCCACTCAAC TTCAATTCCC AGGGCAACTG 
GTGTTAAGCT TTGCTCTCGA GGCTGACAAG GTCTTCATGG AGTTTCGTCG CCCAGATGGG 
AAGAGTTATA CGATAGAAAT GGGTACGAAA GCAGGTCAGC CATCTGTTAT TCGCAGTGGG 
GGTAGTAACC TAGTAGATCC ACAGGACCAA CCATTCCTCG AGTTTGGCAA TGACATTGAC 
CCACCTAACC AACTCGTCAT CGATGTGTTC GAGTCTAGCG TGACTGTTTC TACTGCCCGT 
GGTCAAAAGA TGGTGGCACC TGTTGCAGAA GCTTATCTCT ACAACCATTT CGACATTTGG 
ACGGTGGGTG GTGGTATTGC TCTCGCTGTG GAAGAAATCG AAGATGAAGA TTCGAAAGAT 
TCTCTTGCAA GTAAGACAGA GATTGAAGAA ACAGAACAGG AAATAGAATC AGTAGAAGAG 
GAACTTAAGG GTAACAACCC CGGCGTTCAA CCAGTGCGAA AACGAAAAGG AAAAACAAGC 
GGCAAAAGAC GAGGAAAAAC AGGCGGCAGA AGACGCGGAA AAGCTAAAGA GTCAAAATCA 
GATGACGACA CAGAACCAGA AAACGAACCA GTAGAAGAGG CCAAGGGTGA CAGCCCCAGC 
GTTCAACAAG AGCGAAAACG AAATGGAAAA AAAGGCGGCA AAAGACGAGG AAAAACAGGC 
GACAGAAGAC GCGGAAAAGC TAAAGAGTCA AAATCAGATG ACGACACAGA ACCAGAAAAC 
GAACCAGTAG AAGAGGCCAA GGGTGACAGC CCCAGCGTTC AACAAGAGCG AAAACGAAAT 
GGAAAAAAAG GCGGCAAAAG ACGAGGAAAA ACAGGCGACA GAAGACGCGG AAAAGCTAAA 
GAGTCATCAG ATGACGACAC AGAACCAGAA AACGAACCAG TAGAAGAGGC CAAGGGTGAC 
AGCCCCGGCA TTCAACAAGA GCGAAAACGA AAAGGAAAAA TAGGCGGCAA GAGACGAGGA 
AAAACAGGCG GCAGAATACG CGGAAAAGCT AAAGAGTCAC AATCAGATGA CGACACAGAA 
CCAGAAAACG AACCAGTAGA AGAGGCCAAG GGTGACAGCC CCAGCGTTCA ACAAGAGCGA 
AAACGAAATG GAAAAAAAGG CGGCAAAAGA CGAGGAAAAA CAGGCGACAG AAGACGCGGA 
AAAGCTAAAG AGTCATCAGA TGACGACACA GAACCTGTTA TTGAACCAGT AATACAGGAC 
ACATTAGAAG AAACGAAACC TGTGAACAGT GAAGTATTCG AAGAATTGAA GGGTAACAAC 
CCTGGCGTTG AAGTAAAGAG AACTAGAGGT GACAGAATCA TAGACAATCA AGATATTGAA 
GACACAACAC AAGAGTCATC TCTGACACCA ACTCAAGTTC CACCAACAAT TGAGACAATA 
CGAAACACGG GATCAACCGA TGAGCCAGAG GTCAAAGAGA AAGTTAGCCA GGACGTAGTA 
GAGCCGGTCG TGAAACCGTT CAACGAAGAA CCTGATAAGT CAGCCACTCT TGAGACCAAC 
GTACTTGGCG TCGCTGCGTT CTTCGATCAA ACCTTGGATA TAGATCGTTT ATTACAGCTT 
CAGTTTGATT TCAGTGTCAC CATTAACACC GGTTTCGCTA TCCTCTTGAC ACAAGGACAA 
ATTCTTAGGG GAGATTATTA CTCAATCGGT ATAACCGAGG GAAACGTCTA CATGGTCCAG 
CGATGTAATA AGGAAACAGC GGAATGTAAT CGACTCTTCA GCAGGAAAAT TGATCCACTT 
GGTGAAGGAA CAGGAATATT CACGCTGAAG ATTCAGAAGA TCCTAAATGG CAATGTAGAC 
CCTGATGCTG ATGGAGCATA CTCCTACTGG TTTTCATTGC TGTATGAAGG AGCTGTGGTC 
GGAGAACAGG TGGAAGTACA GTATCCAAAT ACATTCTATA TTAGGTACAT TGGATGCATC 
TCCTACAAGT ACGTGGCCAC GTGGAATCTC TTCTCTGTCG TCTCACCGGG TTCTACCCAG 
AGATCCTCTG CAATCATACT CCGGAACGCC GAAGAAACAA GTAGTCTGGA GGTGAATTCA 
TTTGATCGCG AAGTTGTATT CAGTGTCAAA GCCCAAGATT TTTTGATAAT TCGTCTTTAC 
TCCGTTGGCG ACGTCGTCTA CACAATCCTC GTTGGAGACG AAGGCAATAA CTATGTTACG 
GGATCGTCCG GTACTGGTGA TAAATCTCCG GATGACGCGT CGCGTCGCGT TGAAGGAGGT 
GTGTTAAGCT TGGCGGGCAA GCAGTACTAC CTTTCTTGGC GAAACGGCAG GATGAAGTTC 
GGCGAAACGG AGGAGGACAG CGAGGCGATG ATGAATTGGG AGATTGGGGA AATCTCGGTG 
ACTAAGCTAG AATTCATTGC TGCTTACGAC AGCTTGTATC TCATACACAC ATACAAGTAC 
TTCTACGTAT TTGGCGATGT TTTGCAGGCG ACTCATGATC GAATACGGGC AACTTCTCTC 
ACTTTCTTCA CTCTCCGTCG CGAGCGTGGC GTGCCATACA TCCACCTCAA GATCAAGGCC 
TCTGGGACTG TCTTGATTGC GCTCGCCAAA GACCAGGAGC TTTCTGAATC CGACATGGTT 
TTGATCAAGA TGAGTTCCCG GAGGATAACT CTCTCCACAT GCCTTGGTTG CGAAACAATC 
TACTCGGTTC CGATCCCTAA AGACCAAGCG GCCCTTCTAG ATCCGTCCTC ATTCCTTGAG 
TACCAGATCA CGTGTGAAGG CAACAGTCTG AAGATCTTCT TAACCGGTCA GAAGGAACCC 
TTGCTGGACT ATGGTGGCAT TGATCGAGAC GCCCACTATG GCTATGATGG CTTCGGAGGA 
ACTATCGGTA GCAATTATCA CATCAAGCTC AACCAAGGAA CGATCTACAG AGAGGAGCAT 
TGTCTTGACG TCAACACCTT CTTGGGTGGA GGAACGAAGC TTCTGAAGAT CGTCCACTCT 
GACCAGATGA TTGCTTTATT TGATGCTGAG AAACAGCTCG AGGCAGAGCT TGCCATGGAA 
GATACGAAGA CGGAGCTGCG TACTACCCGT GGTGGCGAGG CCGTTCAGAT AAGCCAAAGT 
AGTAGTTGCA TCTTCCACGC TCCCTACACT CTAGTCTGGA TGGACTTCAG CGGTGGGGCT 
ATTCGGTATG GTACCTACGA CGAGGCGAAT CGCCTGAGAA GTGACCCGTG TATCACCTAC 
GACCTCGGTG ATGATCAGGA GGTCAAATAC ATGTCGTTTG GTCAACAGCA TTCCTCGTGG 
GATGTTACAC ATCACGTGAT TGAGAAGAGG ATTGTGTACA AGGAGTTGGT CTCCCATGTA 
CAAGAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030830441.1
CDS	133..7644
Translation	MNTMNALAFGAVLLLAAVASTSGGGSTSQVADNKERFSYSGGGGLTGLNVKQGGFTQVSHSTKEKTVTTEKETRKSKSKKRKGGKVSTQETSKKETNGGKVKNQRGNTQETSRKRKGRGKNKGNEKTEVKQREGNRGRDGDGKGDRKGRKGSTETVINKERSGGRRRSKNKSGKGSTKGSSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQSVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNAQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDKGSKKDRSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNAQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDKGSKKDRSSKKSGRRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNTQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRDWKMSKCRSGRRQQVIEKLDENESLEYELTTSAESFTIQFCRKREKDCLEIVLSSSGESSIKLDGQDLGDPISDFFYKKGEKFSFTITYTSQRVVVRNSKSGLELVASVDGDELKGFRYCYGTANGGEGDSTFQIREREFSESEISSFEKRYRGKGGRKRGGKRERGNTQSPQERKRGGRKKGDKDSKSNELSGGKKSRGRGGEKRERGNTESPQERKRGDRKKGDKDSKSNEKNRGGRRGGSKKDSKSRKGGRNRGRGRWAHRRGKYYRWRTVKGAVKRTRAVSFDERRSAMSVFIKTKATRFVLEFCLSSGGKDCYTASLSSQRDSASFFEYGGQRTGDSQDGAFIRFKRRTRLYVSVSRTKLTIYNDKGDDLSVALPESSVIQDVYMSTSDGDWTKVRFRLRRSGRTLYSSTGPLARGAAFEDIGSKATSFSCLIRTVASAVTFEICFDSQGNDCYTIELSTQPKQTSRILYGGKLVSNEEIGIFITPSKESKIFFRIEKRQLEIWNKEPNNERQLIAKIRGKGRSFNSIWASTSDGEFTELQMVETEAVREFSSTTTLERTSIATQLQFPGQLVLSFALEADKVFMEFRRPDGKSYTIEMGTKAGQPSVIRSGGSNLVDPQDQPFLEFGNDIDPPNQLVIDVFESSVTVSTARGQKMVAPVAEAYLYNHFDIWTVGGGIALAVEEIEDEDSKDSLASKTEIEETEQEIESVEEELKGNNPGVQPVRKRKGKTSGKRRGKTGGRRRGKAKESKSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESKSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESSDDDTEPENEPVEEAKGDSPGIQQERKRKGKIGGKRRGKTGGRIRGKAKESQSDDDTEPENEPVEEAKGDSPSVQQERKRNGKKGGKRRGKTGDRRRGKAKESSDDDTEPVIEPVIQDTLEETKPVNSEVFEELKGNNPGVEVKRTRGDRIIDNQDIEDTTQESSLTPTQVPPTIETIRNTGSTDEPEVKEKVSQDVVEPVVKPFNEEPDKSATLETNVLGVAAFFDQTLDIDRLLQLQFDFSVTINTGFAILLTQGQILRGDYYSIGITEGNVYMVQRCNKETAECNRLFSRKIDPLGEGTGIFTLKIQKILNGNVDPDADGAYSYWFSLLYEGAVVGEQVEVQYPNTFYIRYIGCISYKYVATWNLFSVVSPGSTQRSSAIILRNAEETSSLEVNSFDREVVFSVKAQDFLIIRLYSVGDVVYTILVGDEGNNYVTGSSGTGDKSPDDASRRVEGGVLSLAGKQYYLSWRNGRMKFGETEEDSEAMMNWEIGEISVTKLEFIAAYDSLYLIHTYKYFYVFGDVLQATHDRIRATSLTFFTLRRERGVPYIHLKIKASGTVLIALAKDQELSESDMVLIKMSSRRITLSTCLGCETIYSVPIPKDQAALLDPSSFLEYQITCEGNSLKIFLTGQKEPLLDYGGIDRDAHYGYDGFGGTIGSNYHIKLNQGTIYREEHCLDVNTFLGGGTKLLKIVHSDQMIALFDAEKQLEAELAMEDTKTELRTTRGGEAVQISQSSSCIFHAPYTLVWMDFSGGAIRYGTYDEANRLRSDPCITYDLGDDQEVKYMSFGQQHSSWDVTHHVIEKRIVYKELVSHVQEP

Target ORF information:

RefSeq Version	XM_030974581.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus uncharacterized LOC576141 (LOC576141), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030974581.1

ORF Insert Sequence:

ATGAACACAA TGAACGCCCT GGCGTTTGGC GCTGTTCTGC TGTTAGCTGC AGTCGCGTCG 
ACCTCAGGCG GCGGTAGCAC CAGTCAAGTT GCTGATAATA AAGAGCGATT CTCATACAGC 
GGCGGAGGTG GTCTCACAGG CCTGAACGTC AAGCAAGGTG GTTTTACTCA GGTTTCCCAC 
TCGACAAAAG AAAAGACTGT CACCACGGAG AAAGAAACTA GGAAGAGCAA ATCAAAAAAG 
CGGAAAGGCG GTAAGGTGTC TACTCAAGAG ACTTCAAAGA AAGAAACTAA CGGGGGCAAA 
GTGAAGAACC AGAGAGGCAA TACGCAAGAG ACTTCACGAA AACGAAAAGG AAGGGGAAAG 
AATAAAGGAA ACGAAAAAAC TGAAGTCAAA CAACGAGAAG GAAATCGCGG TCGAGATGGA 
GATGGCAAAG GAGATCGTAA AGGAAGAAAG GGAAGTACTG AGACGGTGAT CAATAAAGAA 
AGGAGCGGTG GTCGGCGTCG AAGTAAAAAC AAGAGTGGTA AGGGATCGAC CAAAGGATCG 
AGCAGTAAAA AGAGTGGAAG AAGAGATTGG AAGATGTCTA AATGCAGAAG CGGGAGACGA 
CAACAGGTTA TTGAGAAACT GGATGAGAAT GAGAGTCTGG AATATGAGTT GACGACCTCT 
GCTGAATCTT TTACCATCCA GTTCTGTCGA AAGAGAGAAA AAGACTGCCT TGAGATTGTT 
CTGAGCTCAT CTGGTGAATC ATCCATTAAG CTTGATGGAC AGGATTTGGG CGATCCCATT 
TCGGACTTCT TTTACAAGAA AGGGGAGAAG TTCTCCTTCA CTATCACCTA TACATCCCAG 
AGTGTGGTTG TGAGGAACAG CAAATCAGGA TTAGAACTTG TTGCATCGGT CGATGGTGAT 
GAACTTAAGG GTTTCAGGTA CTGCTATGGC ACTGCAAACG GAGGAGAAGG AGATTCTACA 
TTCCAGATAA GGGAACGTGA ATTTTCCGAA TCAGAGATCT CATCATTTGA AAAGCGGTAT 
AGGGGCAAGG GAGGTAGAAA GAGGGGTGGA AAGAGAGAAA GGGGAAACGC CCAGTCACCT 
CAAGAAAGGA AGCGTGGCGG TAGGAAGAAA GGCGACAAAG ACTCAAAGTC GAATGAACTT 
TCCGGCGGCA AAAAGAGCAG AGGAAGAGAT AAAGGATCGA AAAAAGACAG GAGCAGTAAA 
AAGAGTGGAA GAAGAGATTG GAAGATGTCT AAATGCAGAA GCGGGAGACG ACAACAGGTT 
ATTGAGAAAC TGGATGAGAA TGAGAGTCTG GAATATGAGT TGACGACCTC TGCTGAATCT 
TTTACCATCC AGTTCTGTCG AAAGAGAGAA AAAGACTGCC TTGAGATTGT TCTGAGCTCA 
TCTGGTGAAT CATCCATTAA GCTTGATGGA CAGGATTTGG GCGATCCCAT TTCGGACTTC 
TTTTACAAGA AAGGGGAGAA GTTCTCCTTC ACTATCACCT ATACATCCCA GCGTGTGGTT 
GTGAGGAACA GCAAATCAGG ATTAGAACTT GTTGCATCGG TCGATGGTGA TGAACTTAAG 
GGTTTTAGGT ACTGCTATGG CACTGCAAAC GGAGGAGAAG GAGATTCTAC ATTCCAGATA 
AGGGAACGTG AATTTTCCGA ATCAGAGATC TCATCATTTG AAAAGCGGTA TAGGGGCAAG 
GGAGGTAGAA AGAGGGGTGG AAAGAGAGAA AGGGGAAACG CCCAGTCACC TCAAGAAAGG 
AAGCGTGGCG GTAGGAAGAA AGGCGACAAA GACTCAAAGT CGAATGAACT TTCCGGCGGC 
AAAAAGAGCA GAGGAAGAGA TAAAGGATCG AAAAAAGACA GGAGCAGTAA AAAGAGTGGA 
AGAAGAGATT GGAAGATGTC TAAATGCAGA AGCGGGAGAC GACAACAGGT TATTGAGAAA 
CTGGATGAGA ATGAGAGTCT GGAATATGAG TTGACGACCT CTGCTGAATC TTTTACCATC 
CAGTTCTGTC GAAAGAGAGA AAAAGACTGC CTTGAGATTG TTCTGAGCTC ATCTGGTGAA 
TCATCCATTA AGCTTGATGG ACAGGATTTG GGCGATCCCA TTTCGGACTT CTTTTACAAG 
AAAGGGGAGA AGTTCTCCTT CACTATCACC TATACATCCC AGCGTGTGGT TGTGAGGAAC 
AGCAAATCAG GATTAGAACT TGTTGCATCG GTCGATGGTG ATGAACTTAA GGGTTTTAGG 
TACTGCTATG GCACTGCAAA CGGAGGAGAA GGAGATTCTA CATTCCAGAT AAGGGAACGT 
GAATTTTCCG AATCAGAGAT CTCATCATTT GAAAAGCGGT ATAGGGGCAA GGGAGGTAGA 
AAGAGGGGTG GAAAGAGAGA AAGGGGAAAC ACCCAGTCAC CCCAAGAAAG GAAGCGTGGC 
GGTAGGAAGA AAGGCGACAA AGACTCAAAG TCGAATGAAC TTTCCGGCGG CAAAAAGAGC 
AGAGGAAGAG ATTGGAAGAT GTCTAAATGC AGAAGCGGGA GACGACAACA GGTTATTGAG 
AAACTGGATG AGAATGAGAG TCTGGAATAT GAGTTGACGA CCTCTGCTGA ATCTTTTACC 
ATCCAGTTCT GTCGAAAGAG AGAAAAAGAC TGCCTTGAGA TTGTTCTGAG CTCATCTGGT 
GAATCATCCA TTAAGCTTGA TGGACAGGAT TTGGGCGATC CCATTTCGGA CTTCTTTTAC 
AAGAAAGGGG AGAAGTTCTC CTTCACTATC ACCTATACAT CCCAGCGTGT GGTTGTGAGG 
AACAGCAAAT CAGGATTAGA ACTTGTTGCA TCGGTCGATG GTGATGAACT TAAGGGTTTC 
AGGTACTGCT ATGGCACTGC AAACGGAGGA GAAGGAGATT CTACATTCCA GATAAGGGAA 
CGTGAATTTT CCGAATCAGA GATCTCATCA TTTGAAAAGC GGTATAGGGG CAAGGGAGGT 
AGAAAGAGGG GTGGAAAGAG AGAAAGGGGA AACACCCAGT CACCCCAAGA AAGGAAGCGT 
GGCGGTAGGA AGAAAGGCGA CAAAGACTCA AAGTCGAATG AACTTTCCGG CGGCAAAAAG 
AGCAGAGGAA GAGGGGGTGA AAAGAGAGAA AGGGGAAACA CCGAGTCACC CCAAGAAAGG 
AAGCGTGGTG ATCGTAAAAA AGGCGACAAA GACTCAAAGT CGAATGAAAA GAACAGAGGA 
GGACGACGTG GAGGAAGCAA GAAAGACTCT AAGAGCAGAA AGGGCGGACG AAATCGAGGT 
CGTGGAAGAT GGGCACACCG GAGAGGCAAA TACTATCGAT GGCGTACCGT CAAGGGTGCA 
GTGAAGAGGA CACGCGCTGT GAGCTTTGAC GAACGTCGGA GCGCCATGTC TGTTTTTATC 
AAGACCAAAG CAACTCGATT CGTCTTAGAA TTCTGCCTGT CGTCAGGAGG CAAAGACTGC 
TATACTGCTT CCCTGAGTTC ACAGCGTGAT TCGGCCTCCT TCTTCGAGTA CGGCGGCCAA 
AGAACGGGCG ATAGTCAAGA TGGAGCATTC ATTCGATTTA AACGAAGAAC CAGATTATAT 
GTATCAGTCT CACGTACTAA ACTGACGATC TACAATGACA AGGGAGACGA TCTCTCAGTC 
GCTTTACCCG AGTCATCAGT CATCCAAGAT GTCTACATGT CCACATCAGA TGGGGACTGG 
ACGAAGGTTA GATTCCGCCT ACGAAGATCT GGTAGAACCC TTTACAGCTC CACTGGTCCT 
CTTGCTCGCG GTGCTGCCTT CGAAGATATT GGTTCTAAAG CAACGTCATT CTCCTGTCTA 
ATACGCACCG TGGCCAGCGC CGTCACTTTC GAAATTTGCT TCGATTCTCA GGGAAATGAT 
TGTTACACAA TCGAACTGAG CACGCAACCC AAACAGACAT CCCGAATCCT GTATGGAGGA 
AAATTAGTGA GCAACGAAGA AATTGGTATT TTTATTACCC CTAGCAAGGA GTCGAAAATC 
TTCTTTCGCA TCGAGAAACG GCAGCTGGAG ATCTGGAATA AGGAACCAAA CAATGAACGC 
CAACTCATCG CTAAAATCCG CGGAAAAGGT CGCAGTTTTA ATTCCATTTG GGCCTCTACT 
AGTGATGGTG AATTCACCGA GCTTCAGATG GTCGAGACTG AAGCCGTTAG AGAGTTTTCA 
TCCACGACTA CTCTTGAGAG AACATCGATT GCCACTCAAC TTCAATTCCC AGGGCAACTG 
GTGTTAAGCT TTGCTCTCGA GGCTGACAAG GTCTTCATGG AGTTTCGTCG CCCAGATGGG 
AAGAGTTATA CGATAGAAAT GGGTACGAAA GCAGGTCAGC CATCTGTTAT TCGCAGTGGG 
GGTAGTAACC TAGTAGATCC ACAGGACCAA CCATTCCTCG AGTTTGGCAA TGACATTGAC 
CCACCTAACC AACTCGTCAT CGATGTGTTC GAGTCTAGCG TGACTGTTTC TACTGCCCGT 
GGTCAAAAGA TGGTGGCACC TGTTGCAGAA GCTTATCTCT ACAACCATTT CGACATTTGG 
ACGGTGGGTG GTGGTATTGC TCTCGCTGTG GAAGAAATCG AAGATGAAGA TTCGAAAGAT 
TCTCTTGCAA GTAAGACAGA GATTGAAGAA ACAGAACAGG AAATAGAATC AGTAGAAGAG 
GAACTTAAGG GTAACAACCC CGGCGTTCAA CCAGTGCGAA AACGAAAAGG AAAAACAAGC 
GGCAAAAGAC GAGGAAAAAC AGGCGGCAGA AGACGCGGAA AAGCTAAAGA GTCAAAATCA 
GATGACGACA CAGAACCAGA AAACGAACCA GTAGAAGAGG CCAAGGGTGA CAGCCCCAGC 
GTTCAACAAG AGCGAAAACG AAATGGAAAA AAAGGCGGCA AAAGACGAGG AAAAACAGGC 
GACAGAAGAC GCGGAAAAGC TAAAGAGTCA AAATCAGATG ACGACACAGA ACCAGAAAAC 
GAACCAGTAG AAGAGGCCAA GGGTGACAGC CCCAGCGTTC AACAAGAGCG AAAACGAAAT 
GGAAAAAAAG GCGGCAAAAG ACGAGGAAAA ACAGGCGACA GAAGACGCGG AAAAGCTAAA 
GAGTCATCAG ATGACGACAC AGAACCAGAA AACGAACCAG TAGAAGAGGC CAAGGGTGAC 
AGCCCCGGCA TTCAACAAGA GCGAAAACGA AAAGGAAAAA TAGGCGGCAA GAGACGAGGA 
AAAACAGGCG GCAGAATACG CGGAAAAGCT AAAGAGTCAC AATCAGATGA CGACACAGAA 
CCAGAAAACG AACCAGTAGA AGAGGCCAAG GGTGACAGCC CCAGCGTTCA ACAAGAGCGA 
AAACGAAATG GAAAAAAAGG CGGCAAAAGA CGAGGAAAAA CAGGCGACAG AAGACGCGGA 
AAAGCTAAAG AGTCATCAGA TGACGACACA GAACCTGTTA TTGAACCAGT AATACAGGAC 
ACATTAGAAG AAACGAAACC TGTGAACAGT GAAGTATTCG AAGAATTGAA GGGTAACAAC 
CCTGGCGTTG AAGTAAAGAG AACTAGAGGT GACAGAATCA TAGACAATCA AGATATTGAA 
GACACAACAC AAGAGTCATC TCTGACACCA ACTCAAGTTC CACCAACAAT TGAGACAATA 
CGAAACACGG GATCAACCGA TGAGCCAGAG GTCAAAGAGA AAGTTAGCCA GGACGTAGTA 
GAGCCGGTCG TGAAACCGTT CAACGAAGAA CCTGATAAGT CAGCCACTCT TGAGACCAAC 
GTACTTGGCG TCGCTGCGTT CTTCGATCAA ACCTTGGATA TAGATCGTTT ATTACAGCTT 
CAGTTTGATT TCAGTGTCAC CATTAACACC GGTTTCGCTA TCCTCTTGAC ACAAGGACAA 
ATTCTTAGGG GAGATTATTA CTCAATCGGT ATAACCGAGG GAAACGTCTA CATGGTCCAG 
CGATGTAATA AGGAAACAGC GGAATGTAAT CGACTCTTCA GCAGGAAAAT TGATCCACTT 
GGTGAAGGAA CAGGAATATT CACGCTGAAG ATTCAGAAGA TCCTAAATGG CAATGTAGAC 
CCTGATGCTG ATGGAGCATA CTCCTACTGG TTTTCATTGC TGTATGAAGG AGCTGTGGTC 
GGAGAACAGG TGGAAGTACA GTATCCAAAT ACATTCTATA TTAGGTACAT TGGATGCATC 
TCCTACAAGT ACGTGGCCAC GTGGAATCTC TTCTCTGTCG TCTCACCGGG TTCTACCCAG 
AGATCCTCTG CAATCATACT CCGGAACGCC GAAGAAACAA GTAGTCTGGA GGTGAATTCA 
TTTGATCGCG AAGTTGTATT CAGTGTCAAA GCCCAAGATT TTTTGATAAT TCGTCTTTAC 
TCCGTTGGCG ACGTCGTCTA CACAATCCTC GTTGGAGACG AAGGCAATAA CTATGTTACG 
GGATCGTCCG GTACTGGTGA TAAATCTCCG GATGACGCGT CGCGTCGCGT TGAAGGAGGT 
GTGTTAAGCT TGGCGGGCAA GCAGTACTAC CTTTCTTGGC GAAACGGCAG GATGAAGTTC 
GGCGAAACGG AGGAGGACAG CGAGGCGATG ATGAATTGGG AGATTGGGGA AATCTCGGTG 
ACTAAGCTAG AATTCATTGC TGCTTACGAC AGCTTGTATC TCATACACAC ATACAAGTAC 
TTCTACGTAT TTGGCGATGT TTTGCAGGCG ACTCATGATC GAATACGGGC AACTTCTCTC 
ACTTTCTTCA CTCTCCGTCG CGAGCGTGGC GTGCCATACA TCCACCTCAA GATCAAGGCC 
TCTGGGACTG TCTTGATTGC GCTCGCCAAA GACCAGGAGC TTTCTGAATC CGACATGGTT 
TTGATCAAGA TGAGTTCCCG GAGGATAACT CTCTCCACAT GCCTTGGTTG CGAAACAATC 
TACTCGGTTC CGATCCCTAA AGACCAAGCG GCCCTTCTAG ATCCGTCCTC ATTCCTTGAG 
TACCAGATCA CGTGTGAAGG CAACAGTCTG AAGATCTTCT TAACCGGTCA GAAGGAACCC 
TTGCTGGACT ATGGTGGCAT TGATCGAGAC GCCCACTATG GCTATGATGG CTTCGGAGGA 
ACTATCGGTA GCAATTATCA CATCAAGCTC AACCAAGGAA CGATCTACAG AGAGGAGCAT 
TGTCTTGACG TCAACACCTT CTTGGGTGGA GGAACGAAGC TTCTGAAGAT CGTCCACTCT 
GACCAGATGA TTGCTTTATT TGATGCTGAG AAACAGCTCG AGGCAGAGCT TGCCATGGAA 
GATACGAAGA CGGAGCTGCG TACTACCCGT GGTGGCGAGG CCGTTCAGAT AAGCCAAAGT 
AGTAGTTGCA TCTTCCACGC TCCCTACACT CTAGTCTGGA TGGACTTCAG CGGTGGGGCT 
ATTCGGTATG GTACCTACGA CGAGGCGAAT CGCCTGAGAA GTGACCCGTG TATCACCTAC 
GACCTCGGTG ATGATCAGGA GGTCAAATAC ATGTCGTTTG GTCAACAGCA TTCCTCGTGG 
GATGTTACAC ATCACGTGAT TGAGAAGAGG ATTGTGTACA AGGAGTTGGT CTCCCATGTA 
CAAGAGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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1-877-436-7274

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LOC576141 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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