WNK1 gene cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

mRNA	Protein	Name
XM_012550822.1	XP_012406276.1	serine/threonine-protein kinase WNK1 isoform X4
XM_031939533.1	XP_031795393.1	serine/threonine-protein kinase WNK1 isoform X2
XM_031939534.1	XP_031795394.1	serine/threonine-protein kinase WNK1 isoform X3
XM_031939541.1	XP_031795401.1	serine/threonine-protein kinase WNK1 isoform X10
XM_031939538.1	XP_031795398.1	serine/threonine-protein kinase WNK1 isoform X7
XM_012550821.1	XP_012406275.1	serine/threonine-protein kinase WNK1 isoform X3
XM_031939536.1	XP_031795396.1	serine/threonine-protein kinase WNK1 isoform X5
XM_031939537.1	XP_031795397.1	serine/threonine-protein kinase WNK1 isoform X6
XM_023504644.1	XP_023360412.1	LOW QUALITY PROTEIN: serine/threonine-protein kinase WNK1
XM_012550819.1	XP_012406273.1	serine/threonine-protein kinase WNK1 isoform X1
XM_012550820.1	XP_012406274.1	serine/threonine-protein kinase WNK1 isoform X2
XM_012550823.1	XP_012406277.1	serine/threonine-protein kinase WNK1 isoform X5
XM_031939535.1	XP_031795395.1	serine/threonine-protein kinase WNK1 isoform X4
XM_031939540.1	XP_031795400.1	serine/threonine-protein kinase WNK1 isoform X9
XM_031939531.1	XP_031795391.1	serine/threonine-protein kinase WNK1 isoform X1
XM_031939539.1	XP_031795399.1	serine/threonine-protein kinase WNK1 isoform X8
XM_031939542.1	XP_031795402.1	serine/threonine-protein kinase WNK1 isoform X11
XM_031939543.1	XP_031795403.1	serine/threonine-protein kinase WNK1 isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa133593	XM_012550820.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa133594	XM_012550821.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa133595	XM_012550822.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa133596	XM_012550823.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa133592	XM_012550819.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa237672	XM_031939531.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa237673	XM_031939533.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa237674	XM_031939534.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa237675	XM_031939535.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa237676	XM_031939536.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa237677	XM_031939537.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSa237678	XM_031939538.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa237679	XM_031939539.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa237680	XM_031939540.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa237681	XM_031939541.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa237682	XM_031939542.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa237683	XM_031939543.1 Latest version!	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

WNK1 ( XM_012550820.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012406274.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa133593
Clone ID Related Accession (Same CDS sequence)	XM_012550820.1
Accession Version	XM_012550820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003843628.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG AGATGTACGA GGAAAAATAT GATGAGTCCG TTGACGTGTA TGCTTTTGGA 
ATGTGCATGC TTGAGATGGC CACATCCGAA TACCCTTATT CTGAGTGCCA AAATGCTGCG 
CAGATCTACC GTCGAGTGAC CAGTGGAGTA AAGCCAGCCA GCTTTGACAA AGTAGCAATT 
CCTGAAGTAA AGGAAATTAT TGAAGGATGC ATACGCCAAA ACAAAGATGA AAGATATGCT 
ATCAAAGACC TTTTGAACCA TGCCTTCTTC CAGGAGGAAA CAGGAGTACG AGTAGAACTA 
GCTGAAGAAG ATGATGGTGA AAAGATAGCC ATTAAGCTAT GGTTACGCAT TGAAGATATT 
AAGAAATTGA AGGGGAAATA CAAAGACAAT GAAGCAATTG AATTTTCCTT TGATTTGGAG 
AGAGATGTCC CAGAAGATGT AGCTCAAGAA ATGGTAGAAT CAGGGTATGT TTGTGAAGGA 
GATCACAAGA CCATGGCCAA AGCCATCAAA GACAGAGTGT CCCTTATAAA GAGAAAACGA 
GAGCAGCGAC AGTTGGTAAG GGAAGAACAG GAGAAAAGAA AGCAAGAAGA GATCAGTTTA 
AAGGAGCAAG TTGAACAGCC AACAACCATT TCTCAGGCTG GGACCAAATT GATTCCTTCT 
ACCCCTGGCA CAGTTACTGT CTCTACCACA TCAGCCTCAG TTTCCACCCA AGTGGAGCCA 
GAAGAACCAG AGGCAGATCA ACACCAACAA CTGCAGTATC AACAACCTAG TCTCTCCGTG 
TTGTCTGATG GAACAGTTGA CAGTGGTCAG GGGTCTTCAG TCTTCACAGA ATCTCGAGTA 
AGCAGCCAGC AGACTGTCTC ATATGGGTCT CAACATGAAC AGGCACTTTC AACAGGCACG 
ATCCCAAGCT ATACAGCTTC TGTTGTTCAA GCACAATCCC AGCCTCATGG TGTATATCCT 
TCCTCCACCG TGACACCGGC ACAGAGCCAG AGTCATCTGG CTTCAAGTAA CTTAACTGGG 
ATTCCATCTT CCCAGACAGC ACAGCTTGCT CAGCAGGGTA TACAACAAGC AGCCCCTCCT 
CAGCAGACAG GCCAGTATCT TCTTCCCCAG TCAGCAGCAT CTGGTGTGCC TACAACTGCA 
CAGCCTGTGA CGCAGCCCCA GCTTCCACAG GTTCTGCCAA TGCCTCAAGT ATCAGTTGGG 
AAGCAGCTTC CTGTTTCTCA GCCAGTATCG ACCATCCAAG GCGAATCTCA GATACCAGTT 
GCAGCACCTT CCCTTCCACA GCCCTCAGTT AACCCATCTC TCTCGGGTGC TCACTTCCTT 
CCTGTGGGCC AGCCACTTCC AGCATCCTTA ATTCCTCAGT TCTCAGTCTC TCAGATTCCC 
ATGTCAACTC CTCATGTCTC TGTGGCTCAG ACAGGTTTCT CATCCCTGCC AAGCTCTATA 
GCATCTGGCA TTAACCAGCC TCTGCTCACC TTGGCCTCAT CTGCTGCAGC GGCTGCTATC 
CCTGTGGGAT CAACTATGGT CCCTAGTCAG CTTCCCACCC TTCTGCAGCC TGTGACTCAG 
CTGCCAAGTC AGGCTCACCC ACAGCTCCTT CAGCCAGCAG TTCAGTCCAT AGGAATACCA 
GCTAACCTTG GACAAGCTGC TGAGGCTTCA CTTCCCACTG GAGATTCTCT ATACCAGGGA 
TTCCCACCTC GACTGCCACC TCAATACCCA GGAGACTCAA ATATTATTCC CTCATCCACC 
GTGGCTTCTG TTTGCGTCCC TTCTACAGTT CTGTCCCCTC CCTTGCCCAC AGAAGCATTG 
GCTCCACCAG GGTACCTTCC TACAGTTGTG CAGCCATATG TCGAGCCAAG TGTTTTAGTT 
CCTGTGGGTA GTCTAGGAGG ACAGGTTCAA GTACCCCATT CAGCAGTCAC CTTAGCACAG 
CAGGCCCCCT CTGCACCCTC CCAGCCAGCA GCTCTGGAGA CACAAGCAGT TTCCCAGTCC 
AGTCCTCCAG AACCTGTGCC ATCAGCACAG TCACAGACCA CTACATTGGT GTCCTCTATA 
GATAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GGAATGAGAA TGTTCCTTCA 
TCCAGTGGGA GACATGAAGG GAGAACTACA AAACGCCATT ACCGAAAGTC TGTACGGAGT 
CGTTCTCGTC ATGAGAAGAC TGCTCGCCCC AAATTGAGAA TTCTTAATGT GTCAAACAAA 
GGAGACAGAG TGGTGGAATG CCAGTTAGAG ACTCACAACC GGAAAATGGT TACATTCAAA 
TTTGACTTGG ATGGTGACAA TCCTGAGGAG ATAGCATCGA TTATGGTGAA CAATGATTTT 
ATTCTAGCAA CAGAGAGAGA CTCCTTTGTG GATCAGGTGC GAGAAATCAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTTAGTGTG GAACCAGAAG GTGATCAAGG ATCAGAAAGC 
TTACAAACAA AGGATGATTA TGGCTTTTCA GGATCCCAGA AATTGGAGGG AGAGTTCAAG 
CAACCAAATC CTGCATCTTC TATGCCTCAG AGGGCAGGTG TTCCCTCCAG TTCATTAACT 
CAAGTTGTAC ATTCAGCTGG ACGGAGGTTC ATAGTGAGTC CTGTACCAGA GAGTCGGTTA 
CGAGAGTCAG TATTTTTCAC TGCTACATCA AGTGAAATAT CAGACACAAC TGCTGTTGCT 
CCATCCCATG GCCCTGTAAT GAATCTGTCT CATTCTGCTT CATCCGTTAG TTTGCAACAG 
GCATTCTCTG AACTTAGACA TGCTCAAATG TCAGAAGGAC CCAGTACAGC TCCCCCAAAC 
TTCAGCCATG CAGGACCAAC CTTTCCAACT GTACCTGCCA CTATGACTAG CATGGCTGTA 
GTCTTCCCTC CCACGGCAAC AGCTGTGCCT TCAGTATCTG TCCCTGCTAC AAGTAGCCCT 
TTTAATAGTG TTTCTGCACC AGTCATTCAA TCTGTGGTCA CTATGCCTAC TGAAAAGGTA 
ATTGCCGGAG TTGCCTCCAG TGCAGGTGTA TTAACTTCAA GTTCTTTTCC TGTACCACAG 
GTGTCTGGAC CACCAGTAAT GACCAGTGTG GTTTCCAGCA TTACTATTCC TGCAGCCGTG 
TCATTGTCTA CTGCAAGTGA ATCAGTTCAG GCCCCACCAG TTGGGGGAGT TATGTCTAGT 
GCTGGCACTT TTCCTCCTGC AGCGGGATTG GTAGTTCCAG TTCCTGGAAT AGCCAGTACA 
GTTCCCCCAG GTGCTATCAC ACCAGCTATG ACTTCTCAGC CCACCCCAAT TGCAACAAAT 
ATAACTGGGG CAGCCACCCT GACTTCCACA TCTGCCACCA CTCCGTTCCC AAGCATGGCT 
TCACAACCAC CTCTTCAAAT CAGTAGCAGC AGCTCTGCTC CCACTGTAGC AGAAACTGTG 
GTAGTCAGTG CACTCCATCC CAAACACGAT GGCACACATT TACCAGACAA GCCACCCCTT 
AGCAGTGCAG CAGGGCTCTC CCTGCCCCTC TCAGTAGCTC CATCTTCCTC TGGAGCTGGG 
ATCTCTGATT CAGCTAGTCA GCCCAGTGTG CTGCATCCTT TACAGGTCCC ATCCGCTGTG 
GCTTCTCCTC CAGTACTTTC ACAGGCAGCA GGGACCGTCT CCACCCCGGT GTTACCTCAG 
GTACCTTTAC CTGGTATCCC GCCCCTAGCA CAGCCTATTA CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA ACCTCAACCA GCTTCACTTC CCAACCAGCC CCACACTCAT 
TGCCCTGAAG TAGATACTGA TACACAACCC AAAGCCCCTG GCATTGATGA TATAAAGACT 
CTGGAAGAAA AACTGCGGTC TCTCTTCAGT GAACACAGTT CTGTTGGAGC CCAGCATGCC 
CCTGTCTCCC TGGAGACATC ATTGGTAGTG GAGACTACAG TCATGCCAGG CATCCCAACC 
ACCGCTGTGG CACCAAGCAA GCTCATGACT TCCACCACGA GCACTTGCTT ACCACCAAGC 
AGTTTGCCTC TAGGAACAAC TACTGGTTTG TCAGCTGTTA CACCAGTGGT CACACCCGGG 
CAGGCTGCCA CCCCAGTCAG CTATGTTCCA GCCCCCACCA GCACTGCAGT AGGGGTGAAA 
CCTGGAACCC CTCCCTCAAA GCCACCTATC GCCAGGGTAC CGGTACAACC AGTAGGTACT 
GAACTTCAAT CTGGTACCCC ACCCAGCGAG CAGCTGCCGC CTTTTCCAGG ACCTTCCCTA 
ACACAGCCCA CATCAGTTGT GACTCCAACA TCAGTTGCTG GAGCAGTGCC AGTGGTAGTG 
CCAGCTCAGA AAGCAGCTTC AAGCATAGAA GATAATACCA TGATGACCAA CTCAGAGGCT 
AGCGTTCTTA AAATGGGCCG GTTTCAGGTA TCAGTTGCAG TAGATGATTC CTTGAAGGAA 
AATAGAAGCA AGTCGGAAGA AATCAAGTCT GTACACTTTG AATCAAGTAC TTCAGAGTCC 
TCAGTGCTGT CCAGTAGTAG CCCAGAGAAT ACACTGGTGA AGCAGGAGCC TGGTGGAGTC 
ACCACAGCTC TATGTAGCCC CTCTTTGGAT ATGGTTGACG GTGTTCCTTC TCAAAAAACC 
TCTGCTACCC AGACAAAGCC CGAAGCTGAG CAGCCTGCCA CAGTGGTTGG CCGATTCTCT 
GTGTCAAAAA CTGAAGATAA GGTCACCCCT GTGGTGGAGG GAGAGCCACC TGTAGTACGC 
CCTTTATTGG ATTCTGAACA GGCCGTTCCT TCTGCTGTAG CACCAAAGAA AGAAATGCCT 
GAATCGCCAG AGCCAAGCCA TCTTAATGGG CCATCTTGTG ACCTAGAAGC TGCCTTTCTC 
AGTAGGAATA TGGATGATGG TTCAGGTAGC CCCCATTCTA CTCACCATTT GGGCTCAAAA 
GGCAGCCTTC CCATTCAGAG CCTTAGCCAG AGCCTTAGCA ATTCATTCAA CTCCTCTTAC 
ATGAGCAGTG ACAATGAGTC AGACATTGAA GATGAGGACT TGAAGTCAGA ACTACGTCGG 
CTACGAGAAA AGCACCTTAA AGAGATCCAG GACCTACAGA GTCGCCAGAA ACATGAAATC 
GAGTCTTTAT ATACCAAATT GGGCAAAGTC CCTCCTGCAG TCATCATTCC CCCAGCTGCT 
CCACTCTCAG GGAGAAGAAG ACGACCCACT AAAAGCAAAG GCAGCAAATC TAGCCGTAGT 
AGTTCTTTAG GAAATAAGAG CCCCCAACTT TCAGGTAACC TGTCTGCTCA GAGTGCAGCT 
TCAGTCTTGC ACCCCCAGCA GACGCTCCAC CCTCCAGGCA GCATCCCAGA GACTGGGCAG 
AATCAGCTGT TACAGCCCCT TAAACCATCT CCCTCCAGTG AGAACCTCTA TTCAGCTTTT 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGCCAAGG GACCAGCAGT 
ACGAATACAG TGGGAGGAGC AGTCAATAGC CAAGCCCCAC AAGCCCAGCC GCCCAGTAGA 
AAGGGCACAT TTACAGATGA TCTACACAAG TTGGTAGACA ACTGGGCCCG AGATGCCATG 
AATCTCTCAG GCAGGAGAGG AAGCAAGGGG CACATGAACT ATGAGGGTCC TGGAATGGCA 
AGGAAATTTT CAGCACCTGG ACAGCTCTGC ATCTCCATGA CCTCTAATCT GGGTGGTTCT 
GCCCCCATTT CTGCAGCATC AGCTACCTCT TTAGGTCACT TCACCAAGGC TATGTGCCCC 
CCACAGCAGT ACAGCTTTCC ATCTGCCCCT TTCAGCACTC AGTGGAGTGG GACAAGTGGC 
CCAGCACCGC CACCCCTCAG CCAGTTCCAG CCTGTAGGGA CTTCGTCCTT GCAGAGTTTC 
AACATCAGCA ATTTGCAAAA ATCCATCAGC AATCCTCCAG GTTCAAACCT GAGGACAACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012406274.1
CDS	24..5966
Translation	MAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_012550820.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012550820.1

ORF Insert Sequence:

ATGGCCCCTG AGATGTACGA GGAAAAATAT GATGAGTCCG TTGACGTGTA TGCTTTTGGA 
ATGTGCATGC TTGAGATGGC CACATCCGAA TACCCTTATT CTGAGTGCCA AAATGCTGCG 
CAGATCTACC GTCGAGTGAC CAGTGGAGTA AAGCCAGCCA GCTTTGACAA AGTAGCAATT 
CCTGAAGTAA AGGAAATTAT TGAAGGATGC ATACGCCAAA ACAAAGATGA AAGATATGCT 
ATCAAAGACC TTTTGAACCA TGCCTTCTTC CAGGAGGAAA CAGGAGTACG AGTAGAACTA 
GCTGAAGAAG ATGATGGTGA AAAGATAGCC ATTAAGCTAT GGTTACGCAT TGAAGATATT 
AAGAAATTGA AGGGGAAATA CAAAGACAAT GAAGCAATTG AATTTTCCTT TGATTTGGAG 
AGAGATGTCC CAGAAGATGT AGCTCAAGAA ATGGTAGAAT CAGGGTATGT TTGTGAAGGA 
GATCACAAGA CCATGGCCAA AGCCATCAAA GACAGAGTGT CCCTTATAAA GAGAAAACGA 
GAGCAGCGAC AGTTGGTAAG GGAAGAACAG GAGAAAAGAA AGCAAGAAGA GATCAGTTTA 
AAGGAGCAAG TTGAACAGCC AACAACCATT TCTCAGGCTG GGACCAAATT GATTCCTTCT 
ACCCCTGGCA CAGTTACTGT CTCTACCACA TCAGCCTCAG TTTCCACCCA AGTGGAGCCA 
GAAGAACCAG AGGCAGATCA ACACCAACAA CTGCAGTATC AACAACCTAG TCTCTCCGTG 
TTGTCTGATG GAACAGTTGA CAGTGGTCAG GGGTCTTCAG TCTTCACAGA ATCTCGAGTA 
AGCAGCCAGC AGACTGTCTC ATATGGGTCT CAACATGAAC AGGCACTTTC AACAGGCACG 
ATCCCAAGCT ATACAGCTTC TGTTGTTCAA GCACAATCCC AGCCTCATGG TGTATATCCT 
TCCTCCACCG TGACACCGGC ACAGAGCCAG AGTCATCTGG CTTCAAGTAA CTTAACTGGG 
ATTCCATCTT CCCAGACAGC ACAGCTTGCT CAGCAGGGTA TACAACAAGC AGCCCCTCCT 
CAGCAGACAG GCCAGTATCT TCTTCCCCAG TCAGCAGCAT CTGGTGTGCC TACAACTGCA 
CAGCCTGTGA CGCAGCCCCA GCTTCCACAG GTTCTGCCAA TGCCTCAAGT ATCAGTTGGG 
AAGCAGCTTC CTGTTTCTCA GCCAGTATCG ACCATCCAAG GCGAATCTCA GATACCAGTT 
GCAGCACCTT CCCTTCCACA GCCCTCAGTT AACCCATCTC TCTCGGGTGC TCACTTCCTT 
CCTGTGGGCC AGCCACTTCC AGCATCCTTA ATTCCTCAGT TCTCAGTCTC TCAGATTCCC 
ATGTCAACTC CTCATGTCTC TGTGGCTCAG ACAGGTTTCT CATCCCTGCC AAGCTCTATA 
GCATCTGGCA TTAACCAGCC TCTGCTCACC TTGGCCTCAT CTGCTGCAGC GGCTGCTATC 
CCTGTGGGAT CAACTATGGT CCCTAGTCAG CTTCCCACCC TTCTGCAGCC TGTGACTCAG 
CTGCCAAGTC AGGCTCACCC ACAGCTCCTT CAGCCAGCAG TTCAGTCCAT AGGAATACCA 
GCTAACCTTG GACAAGCTGC TGAGGCTTCA CTTCCCACTG GAGATTCTCT ATACCAGGGA 
TTCCCACCTC GACTGCCACC TCAATACCCA GGAGACTCAA ATATTATTCC CTCATCCACC 
GTGGCTTCTG TTTGCGTCCC TTCTACAGTT CTGTCCCCTC CCTTGCCCAC AGAAGCATTG 
GCTCCACCAG GGTACCTTCC TACAGTTGTG CAGCCATATG TCGAGCCAAG TGTTTTAGTT 
CCTGTGGGTA GTCTAGGAGG ACAGGTTCAA GTACCCCATT CAGCAGTCAC CTTAGCACAG 
CAGGCCCCCT CTGCACCCTC CCAGCCAGCA GCTCTGGAGA CACAAGCAGT TTCCCAGTCC 
AGTCCTCCAG AACCTGTGCC ATCAGCACAG TCACAGACCA CTACATTGGT GTCCTCTATA 
GATAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GGAATGAGAA TGTTCCTTCA 
TCCAGTGGGA GACATGAAGG GAGAACTACA AAACGCCATT ACCGAAAGTC TGTACGGAGT 
CGTTCTCGTC ATGAGAAGAC TGCTCGCCCC AAATTGAGAA TTCTTAATGT GTCAAACAAA 
GGAGACAGAG TGGTGGAATG CCAGTTAGAG ACTCACAACC GGAAAATGGT TACATTCAAA 
TTTGACTTGG ATGGTGACAA TCCTGAGGAG ATAGCATCGA TTATGGTGAA CAATGATTTT 
ATTCTAGCAA CAGAGAGAGA CTCCTTTGTG GATCAGGTGC GAGAAATCAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTTAGTGTG GAACCAGAAG GTGATCAAGG ATCAGAAAGC 
TTACAAACAA AGGATGATTA TGGCTTTTCA GGATCCCAGA AATTGGAGGG AGAGTTCAAG 
CAACCAAATC CTGCATCTTC TATGCCTCAG AGGGCAGGTG TTCCCTCCAG TTCATTAACT 
CAAGTTGTAC ATTCAGCTGG ACGGAGGTTC ATAGTGAGTC CTGTACCAGA GAGTCGGTTA 
CGAGAGTCAG TATTTTTCAC TGCTACATCA AGTGAAATAT CAGACACAAC TGCTGTTGCT 
CCATCCCATG GCCCTGTAAT GAATCTGTCT CATTCTGCTT CATCCGTTAG TTTGCAACAG 
GCATTCTCTG AACTTAGACA TGCTCAAATG TCAGAAGGAC CCAGTACAGC TCCCCCAAAC 
TTCAGCCATG CAGGACCAAC CTTTCCAACT GTACCTGCCA CTATGACTAG CATGGCTGTA 
GTCTTCCCTC CCACGGCAAC AGCTGTGCCT TCAGTATCTG TCCCTGCTAC AAGTAGCCCT 
TTTAATAGTG TTTCTGCACC AGTCATTCAA TCTGTGGTCA CTATGCCTAC TGAAAAGGTA 
ATTGCCGGAG TTGCCTCCAG TGCAGGTGTA TTAACTTCAA GTTCTTTTCC TGTACCACAG 
GTGTCTGGAC CACCAGTAAT GACCAGTGTG GTTTCCAGCA TTACTATTCC TGCAGCCGTG 
TCATTGTCTA CTGCAAGTGA ATCAGTTCAG GCCCCACCAG TTGGGGGAGT TATGTCTAGT 
GCTGGCACTT TTCCTCCTGC AGCGGGATTG GTAGTTCCAG TTCCTGGAAT AGCCAGTACA 
GTTCCCCCAG GTGCTATCAC ACCAGCTATG ACTTCTCAGC CCACCCCAAT TGCAACAAAT 
ATAACTGGGG CAGCCACCCT GACTTCCACA TCTGCCACCA CTCCGTTCCC AAGCATGGCT 
TCACAACCAC CTCTTCAAAT CAGTAGCAGC AGCTCTGCTC CCACTGTAGC AGAAACTGTG 
GTAGTCAGTG CACTCCATCC CAAACACGAT GGCACACATT TACCAGACAA GCCACCCCTT 
AGCAGTGCAG CAGGGCTCTC CCTGCCCCTC TCAGTAGCTC CATCTTCCTC TGGAGCTGGG 
ATCTCTGATT CAGCTAGTCA GCCCAGTGTG CTGCATCCTT TACAGGTCCC ATCCGCTGTG 
GCTTCTCCTC CAGTACTTTC ACAGGCAGCA GGGACCGTCT CCACCCCGGT GTTACCTCAG 
GTACCTTTAC CTGGTATCCC GCCCCTAGCA CAGCCTATTA CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA ACCTCAACCA GCTTCACTTC CCAACCAGCC CCACACTCAT 
TGCCCTGAAG TAGATACTGA TACACAACCC AAAGCCCCTG GCATTGATGA TATAAAGACT 
CTGGAAGAAA AACTGCGGTC TCTCTTCAGT GAACACAGTT CTGTTGGAGC CCAGCATGCC 
CCTGTCTCCC TGGAGACATC ATTGGTAGTG GAGACTACAG TCATGCCAGG CATCCCAACC 
ACCGCTGTGG CACCAAGCAA GCTCATGACT TCCACCACGA GCACTTGCTT ACCACCAAGC 
AGTTTGCCTC TAGGAACAAC TACTGGTTTG TCAGCTGTTA CACCAGTGGT CACACCCGGG 
CAGGCTGCCA CCCCAGTCAG CTATGTTCCA GCCCCCACCA GCACTGCAGT AGGGGTGAAA 
CCTGGAACCC CTCCCTCAAA GCCACCTATC GCCAGGGTAC CGGTACAACC AGTAGGTACT 
GAACTTCAAT CTGGTACCCC ACCCAGCGAG CAGCTGCCGC CTTTTCCAGG ACCTTCCCTA 
ACACAGCCCA CATCAGTTGT GACTCCAACA TCAGTTGCTG GAGCAGTGCC AGTGGTAGTG 
CCAGCTCAGA AAGCAGCTTC AAGCATAGAA GATAATACCA TGATGACCAA CTCAGAGGCT 
AGCGTTCTTA AAATGGGCCG GTTTCAGGTA TCAGTTGCAG TAGATGATTC CTTGAAGGAA 
AATAGAAGCA AGTCGGAAGA AATCAAGTCT GTACACTTTG AATCAAGTAC TTCAGAGTCC 
TCAGTGCTGT CCAGTAGTAG CCCAGAGAAT ACACTGGTGA AGCAGGAGCC TGGTGGAGTC 
ACCACAGCTC TATGTAGCCC CTCTTTGGAT ATGGTTGACG GTGTTCCTTC TCAAAAAACC 
TCTGCTACCC AGACAAAGCC CGAAGCTGAG CAGCCTGCCA CAGTGGTTGG CCGATTCTCT 
GTGTCAAAAA CTGAAGATAA GGTCACCCCT GTGGTGGAGG GAGAGCCACC TGTAGTACGC 
CCTTTATTGG ATTCTGAACA GGCCGTTCCT TCTGCTGTAG CACCAAAGAA AGAAATGCCT 
GAATCGCCAG AGCCAAGCCA TCTTAATGGG CCATCTTGTG ACCTAGAAGC TGCCTTTCTC 
AGTAGGAATA TGGATGATGG TTCAGGTAGC CCCCATTCTA CTCACCATTT GGGCTCAAAA 
GGCAGCCTTC CCATTCAGAG CCTTAGCCAG AGCCTTAGCA ATTCATTCAA CTCCTCTTAC 
ATGAGCAGTG ACAATGAGTC AGACATTGAA GATGAGGACT TGAAGTCAGA ACTACGTCGG 
CTACGAGAAA AGCACCTTAA AGAGATCCAG GACCTACAGA GTCGCCAGAA ACATGAAATC 
GAGTCTTTAT ATACCAAATT GGGCAAAGTC CCTCCTGCAG TCATCATTCC CCCAGCTGCT 
CCACTCTCAG GGAGAAGAAG ACGACCCACT AAAAGCAAAG GCAGCAAATC TAGCCGTAGT 
AGTTCTTTAG GAAATAAGAG CCCCCAACTT TCAGGTAACC TGTCTGCTCA GAGTGCAGCT 
TCAGTCTTGC ACCCCCAGCA GACGCTCCAC CCTCCAGGCA GCATCCCAGA GACTGGGCAG 
AATCAGCTGT TACAGCCCCT TAAACCATCT CCCTCCAGTG AGAACCTCTA TTCAGCTTTT 
ACCAGTGATG GTGCCATTTC AGTACCAAGC CTTTCTGCTC CAGGCCAAGG GACCAGCAGT 
ACGAATACAG TGGGAGGAGC AGTCAATAGC CAAGCCCCAC AAGCCCAGCC GCCCAGTAGA 
AAGGGCACAT TTACAGATGA TCTACACAAG TTGGTAGACA ACTGGGCCCG AGATGCCATG 
AATCTCTCAG GCAGGAGAGG AAGCAAGGGG CACATGAACT ATGAGGGTCC TGGAATGGCA 
AGGAAATTTT CAGCACCTGG ACAGCTCTGC ATCTCCATGA CCTCTAATCT GGGTGGTTCT 
GCCCCCATTT CTGCAGCATC AGCTACCTCT TTAGGTCACT TCACCAAGGC TATGTGCCCC 
CCACAGCAGT ACAGCTTTCC ATCTGCCCCT TTCAGCACTC AGTGGAGTGG GACAAGTGGC 
CCAGCACCGC CACCCCTCAG CCAGTTCCAG CCTGTAGGGA CTTCGTCCTT GCAGAGTTTC 
AACATCAGCA ATTTGCAAAA ATCCATCAGC AATCCTCCAG GTTCAAACCT GAGGACAACT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_012550821.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012406275.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

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CloneID	OSa133594
Clone ID Related Accession (Same CDS sequence)	XM_012550821.1
Accession Version	XM_012550821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003843628.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG AGATGTACGA GGAAAAATAT GATGAGTCCG TTGACGTGTA TGCTTTTGGA 
ATGTGCATGC TTGAGATGGC CACATCCGAA TACCCTTATT CTGAGTGCCA AAATGCTGCG 
CAGATCTACC GTCGAGTGAC CAGTGGAGTA AAGCCAGCCA GCTTTGACAA AGTAGCAATT 
CCTGAAGTAA AGGAAATTAT TGAAGGATGC ATACGCCAAA ACAAAGATGA AAGATATGCT 
ATCAAAGACC TTTTGAACCA TGCCTTCTTC CAGGAGGAAA CAGGAGTACG AGTAGAACTA 
GCTGAAGAAG ATGATGGTGA AAAGATAGCC ATTAAGCTAT GGTTACGCAT TGAAGATATT 
AAGAAATTGA AGGGGAAATA CAAAGACAAT GAAGCAATTG AATTTTCCTT TGATTTGGAG 
AGAGATGTCC CAGAAGATGT AGCTCAAGAA ATGGTAGAAT CAGGGTATGT TTGTGAAGGA 
GATCACAAGA CCATGGCCAA AGCCATCAAA GACAGAGTGT CCCTTATAAA GAGAAAACGA 
GAGCAGCGAC AGTTGGTAAG GGAAGAACAG GAGAAAAGAA AGCAAGAAGA GATCAGTTTA 
AAGGAGCAAG TTGAACAGCC AACAACCATT TCTCAGGCTG GGACCAAATT GATTCCTTCT 
ACCCCTGGCA CAGTTACTGT CTCTACCACA TCAGCCTCAG TTTCCACCCA AGTGGAGCCA 
GAAGAACCAG AGGCAGATCA ACACCAACAA CTGCAGTATC AACAACCTAG TCTCTCCGTG 
TTGTCTGATG GAACAGTTGA CAGTGGTCAG GGGTCTTCAG TCTTCACAGA ATCTCGAGTA 
AGCAGCCAGC AGACTGTCTC ATATGGGTCT CAACATGAAC AGGCACTTTC AACAGGCACG 
ATCCCAAGCT ATACAGCTTC TGTTGTTCAA GCACAATCCC AGCCTCATGG TGTATATCCT 
TCCTCCACCG TGACACCGGC ACAGAGCCAG AGTCATCTGG CTTCAAGTAA CTTAACTGGG 
ATTCCATCTT CCCAGACAGC ACAGCTTGCT CAGCAGGGTA TACAACAAGC AGCCCCTCCT 
CAGCAGACAG GCCAGTATCT TCTTCCCCAG TCAGCAGCAT CTGGTGTGCC TACAACTGCA 
CAGCCTGTGA CGCAGCCCCA GCTTCCACAG GTTCTGCCAA TGCCTCAAGT ATCAGTTGGG 
AAGCAGCTTC CTGTTTCTCA GCCAGTATCG ACCATCCAAG GCGAATCTCA GATACCAGTT 
GCAGCACCTT CCCTTCCACA GCCCTCAGTT AACCCATCTC TCTCGGGTGC TCACTTCCTT 
CCTGTGGGCC AGCCACTTCC AGCATCCTTA ATTCCTCAGT TCTCAGTCTC TCAGATTCCC 
ATGTCAACTC CTCATGTCTC TGTGGCTCAG ACAGGTTTCT CATCCCTGCC AAGCTCTATA 
GCATCTGGCA TTAACCAGCC TCTGCTCACC TTGGCCTCAT CTGCTGCAGC GGCTGCTATC 
CCTGTGGGAT CAACTATGGT CCCTAGTCAG CTTCCCACCC TTCTGCAGCC TGTGACTCAG 
CTGCCAAGTC AGGCTCACCC ACAGCTCCTT CAGCCAGCAG TTCAGTCCAT AGGAATACCA 
GCTAACCTTG GACAAGCTGC TGAGGCTTCA CTTCCCACTG GAGATTCTCT ATACCAGGGA 
TTCCCACCTC GACTGCCACC TCAATACCCA GGAGACTCAA ATATTATTCC CTCATCCACC 
GTGGCTTCTG TTTGCGTCCC TTCTACAGTT CTGTCCCCTC CCTTGCCCAC AGAAGCATTG 
GCTCCACCAG GGTACCTTCC TACAGTTGTG CAGCCATATG TCGAGCCAAG TGTTTTAGTT 
CCTGTGGGTA GTCTAGGAGG ACAGGTTCAA GTACCCCATT CAGCAGTCAC CTTAGCACAG 
CAGGCCCCCT CTGCACCCTC CCAGCCAGCA GCTCTGGAGA CACAAGCAGT TTCCCAGTCC 
AGTCCTCCAG AACCTGTGCC ATCAGCACAG TCACAGACCA CTACATTGGT GTCCTCTATA 
GATAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GGAATGAGAA TGTTCCTTCA 
TCCAGTGGGA GACATGAAGG GAGAACTACA AAACGCCATT ACCGAAAGTC TGTACGGAGT 
CGTTCTCGTC ATGAGAAGAC TGCTCGCCCC AAATTGAGAA TTCTTAATGT GTCAAACAAA 
GGAGACAGAG TGGTGGAATG CCAGTTAGAG ACTCACAACC GGAAAATGGT TACATTCAAA 
TTTGACTTGG ATGGTGACAA TCCTGAGGAG ATAGCATCGA TTATGGTGAA CAATGATTTT 
ATTCTAGCAA CAGAGAGAGA CTCCTTTGTG GATCAGGTGC GAGAAATCAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTTAGTGTG GAACCAGAAG GTGATCAAGG ATCAGAAAGC 
TTACAAACAA AGGATGATTA TGGCTTTTCA GGATCCCAGA AATTGGAGGG AGAGTTCAAG 
CAACCAAATC CTGCATCTTC TATGCCTCAG AGGGCAGGTG TTCCCTCCAG TTCATTAACT 
CAAGTTGTAC ATTCAGCTGG ACGGAGGTTC ATAGTGAGTC CTGTACCAGA GAGTCGGTTA 
CGAGAGTCAG TATTTTTCAC TGCTACATCA AGTGAAATAT CAGACACAAC TGCTGTTGCT 
CCATCCCATG GCCCTGTAAT GAATCTGTCT CATTCTGCTT CATCCGTTAG TTTGCAACAG 
GCATTCTCTG AACTTAGACA TGCTCAAATG TCAGAAGGAC CCAGTACAGC TCCCCCAAAC 
TTCAGCCATG CAGGACCAAC CTTTCCAACT GTACCTGCCA CTATGACTAG CATGGCTGTA 
GTCTTCCCTC CCACGGCAAC AGCTGTGCCT TCAGTATCTG TCCCTGCTAC AAGTAGCCCT 
TTTAATAGTG TTTCTGCACC AGTCATTCAA TCTGTGGTCA CTATGCCTAC TGAAAAGGTA 
ATTGCCGGAG TTGCCTCCAG TGCAGGTGTA TTAACTTCAA GTTCTTTTCC TGTACCACAG 
GTGTCTGGAC CACCAGTAAT GACCAGTGTG GTTTCCAGCA TTACTATTCC TGCAGCCGTG 
TCATTGTCTA CTGCAAGTGA ATCAGTTCAG GCCCCACCAG TTGGGGGAGT TATGTCTAGT 
GCTGGCACTT TTCCTCCTGC AGCGGGATTG GTAGTTCCAG TTCCTGGAAT AGCCAGTACA 
GTTCCCCCAG GTGCTATCAC ACCAGCTATG ACTTCTCAGC CCACCCCAAT TGCAACAAAT 
ATAACTGGGG CAGCCACCCT GACTTCCACA TCTGCCACCA CTCCGTTCCC AAGCATGGCT 
TCACAACCAC CTCTTCAAAT CAGTAGCAGC AGCTCTGCTC CCACTGTAGC AGAAACTGTG 
GTAGTCAGTG CACTCCATCC CAAACACGAT GGCACACATT TACCAGACAA GCCACCCCTT 
AGCAGTGCAG CAGGGCTCTC CCTGCCCCTC TCAGTAGCTC CATCTTCCTC TGGAGCTGGG 
ATCTCTGATT CAGCTAGTCA GCCCAGTGTG CTGCATCCTT TACAGGTCCC ATCCGCTGTG 
GCTTCTCCTC CAGTACTTTC ACAGGCAGCA GGGACCGTCT CCACCCCGGT GTTACCTCAG 
GTACCTTTAC CTGGTATCCC GCCCCTAGCA CAGCCTATTA CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA ACCTCAACCA GCTTCACTTC CCAACCAGCC CCACACTCAT 
TGCCCTGAAG TAGATACTGA TACACAACCC AAAGCCCCTG GCATTGATGA TATAAAGACT 
CTGGAAGAAA AACTGCGGTC TCTCTTCAGT GAACACAGTT CTGTTGGAGC CCAGCATGCC 
CCTGTCTCCC TGGAGACATC ATTGGTAGTG GAGACTACAG TCATGCCAGG CATCCCAACC 
ACCGCTGTGG CACCAAGCAA GCTCATGACT TCCACCACGA GCACTTGCTT ACCACCAAGC 
AGTTTGCCTC TAGGAACAAC TACTGGTTTG TCAGCTGTTA CACCAGTGGT CACACCCGGG 
CAGGCTGCCA CCCCAGTCAG CTATGTTCCA GCCCCCACCA GCACTGCAGT AGGGGTGAAA 
CCTGGAACCC CTCCCTCAAA GCCACCTATC GCCAGGGTAC CGGTACAACC AGTAGGTACT 
GAACTTCAAT CTGGTACCCC ACCCAGCGAG CAGCTGCCGC CTTTTCCAGG ACCTTCCCTA 
ACACAGCCCA CATCAGTTGT GACTCCAACA TCAGTTGCTG GAGCAGTGCC AGTGGTAGTG 
CCAGCTCAGA AAGCTTCAAG CATAGAAGAT AATACCATGA TGACCAACTC AGAGGCTAGC 
GTTCTTAAAA TGGGCCGGTT TCAGGTATCA GTTGCAGTAG ATGATTCCTT GAAGGAAAAT 
AGAAGCAAGT CGGAAGAAAT CAAGTCTGTA CACTTTGAAT CAAGTACTTC AGAGTCCTCA 
GTGCTGTCCA GTAGTAGCCC AGAGAATACA CTGGTGAAGC AGGAGCCTGG TGGAGTCACC 
ACAGCTCTAT GTAGCCCCTC TTTGGATATG GTTGACGGTG TTCCTTCTCA AAAAACCTCT 
GCTACCCAGA CAAAGCCCGA AGCTGAGCAG CCTGCCACAG TGGTTGGCCG ATTCTCTGTG 
TCAAAAACTG AAGATAAGGT CACCCCTGTG GTGGAGGGAG AGCCACCTGT AGTACGCCCT 
TTATTGGATT CTGAACAGGC CGTTCCTTCT GCTGTAGCAC CAAAGAAAGA AATGCCTGAA 
TCGCCAGAGC CAAGCCATCT TAATGGGCCA TCTTGTGACC TAGAAGCTGC CTTTCTCAGT 
AGGAATATGG ATGATGGTTC AGGTAGCCCC CATTCTACTC ACCATTTGGG CTCAAAAGGC 
AGCCTTCCCA TTCAGAGCCT TAGCCAGAGC CTTAGCAATT CATTCAACTC CTCTTACATG 
AGCAGTGACA ATGAGTCAGA CATTGAAGAT GAGGACTTGA AGTCAGAACT ACGTCGGCTA 
CGAGAAAAGC ACCTTAAAGA GATCCAGGAC CTACAGAGTC GCCAGAAACA TGAAATCGAG 
TCTTTATATA CCAAATTGGG CAAAGTCCCT CCTGCAGTCA TCATTCCCCC AGCTGCTCCA 
CTCTCAGGGA GAAGAAGACG ACCCACTAAA AGCAAAGGCA GCAAATCTAG CCGTAGTAGT 
TCTTTAGGAA ATAAGAGCCC CCAACTTTCA GGTAACCTGT CTGCTCAGAG TGCAGCTTCA 
GTCTTGCACC CCCAGCAGAC GCTCCACCCT CCAGGCAGCA TCCCAGAGAC TGGGCAGAAT 
CAGCTGTTAC AGCCCCTTAA ACCATCTCCC TCCAGTGAGA ACCTCTATTC AGCTTTTACC 
AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GCCAAGGGAC CAGCAGTACG 
AATACAGTGG GAGGAGCAGT CAATAGCCAA GCCCCACAAG CCCAGCCGCC CAGTAGAAAG 
GGCACATTTA CAGATGATCT ACACAAGTTG GTAGACAACT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GGAGAGGAAG CAAGGGGCAC ATGAACTATG AGGGTCCTGG AATGGCAAGG 
AAATTTTCAG CACCTGGACA GCTCTGCATC TCCATGACCT CTAATCTGGG TGGTTCTGCC 
CCCATTTCTG CAGCATCAGC TACCTCTTTA GGTCACTTCA CCAAGGCTAT GTGCCCCCCA 
CAGCAGTACA GCTTTCCATC TGCCCCTTTC AGCACTCAGT GGAGTGGGAC AAGTGGCCCA 
GCACCGCCAC CCCTCAGCCA GTTCCAGCCT GTAGGGACTT CGTCCTTGCA GAGTTTCAAC 
ATCAGCAATT TGCAAAAATC CATCAGCAAT CCTCCAGGTT CAAACCTGAG GACAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012406275.1
CDS	24..5963
Translation	MAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQPTSVVTPTSVAGAVPVVVPAQKASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_012550821.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012550821.1

ORF Insert Sequence:

ATGGCCCCTG AGATGTACGA GGAAAAATAT GATGAGTCCG TTGACGTGTA TGCTTTTGGA 
ATGTGCATGC TTGAGATGGC CACATCCGAA TACCCTTATT CTGAGTGCCA AAATGCTGCG 
CAGATCTACC GTCGAGTGAC CAGTGGAGTA AAGCCAGCCA GCTTTGACAA AGTAGCAATT 
CCTGAAGTAA AGGAAATTAT TGAAGGATGC ATACGCCAAA ACAAAGATGA AAGATATGCT 
ATCAAAGACC TTTTGAACCA TGCCTTCTTC CAGGAGGAAA CAGGAGTACG AGTAGAACTA 
GCTGAAGAAG ATGATGGTGA AAAGATAGCC ATTAAGCTAT GGTTACGCAT TGAAGATATT 
AAGAAATTGA AGGGGAAATA CAAAGACAAT GAAGCAATTG AATTTTCCTT TGATTTGGAG 
AGAGATGTCC CAGAAGATGT AGCTCAAGAA ATGGTAGAAT CAGGGTATGT TTGTGAAGGA 
GATCACAAGA CCATGGCCAA AGCCATCAAA GACAGAGTGT CCCTTATAAA GAGAAAACGA 
GAGCAGCGAC AGTTGGTAAG GGAAGAACAG GAGAAAAGAA AGCAAGAAGA GATCAGTTTA 
AAGGAGCAAG TTGAACAGCC AACAACCATT TCTCAGGCTG GGACCAAATT GATTCCTTCT 
ACCCCTGGCA CAGTTACTGT CTCTACCACA TCAGCCTCAG TTTCCACCCA AGTGGAGCCA 
GAAGAACCAG AGGCAGATCA ACACCAACAA CTGCAGTATC AACAACCTAG TCTCTCCGTG 
TTGTCTGATG GAACAGTTGA CAGTGGTCAG GGGTCTTCAG TCTTCACAGA ATCTCGAGTA 
AGCAGCCAGC AGACTGTCTC ATATGGGTCT CAACATGAAC AGGCACTTTC AACAGGCACG 
ATCCCAAGCT ATACAGCTTC TGTTGTTCAA GCACAATCCC AGCCTCATGG TGTATATCCT 
TCCTCCACCG TGACACCGGC ACAGAGCCAG AGTCATCTGG CTTCAAGTAA CTTAACTGGG 
ATTCCATCTT CCCAGACAGC ACAGCTTGCT CAGCAGGGTA TACAACAAGC AGCCCCTCCT 
CAGCAGACAG GCCAGTATCT TCTTCCCCAG TCAGCAGCAT CTGGTGTGCC TACAACTGCA 
CAGCCTGTGA CGCAGCCCCA GCTTCCACAG GTTCTGCCAA TGCCTCAAGT ATCAGTTGGG 
AAGCAGCTTC CTGTTTCTCA GCCAGTATCG ACCATCCAAG GCGAATCTCA GATACCAGTT 
GCAGCACCTT CCCTTCCACA GCCCTCAGTT AACCCATCTC TCTCGGGTGC TCACTTCCTT 
CCTGTGGGCC AGCCACTTCC AGCATCCTTA ATTCCTCAGT TCTCAGTCTC TCAGATTCCC 
ATGTCAACTC CTCATGTCTC TGTGGCTCAG ACAGGTTTCT CATCCCTGCC AAGCTCTATA 
GCATCTGGCA TTAACCAGCC TCTGCTCACC TTGGCCTCAT CTGCTGCAGC GGCTGCTATC 
CCTGTGGGAT CAACTATGGT CCCTAGTCAG CTTCCCACCC TTCTGCAGCC TGTGACTCAG 
CTGCCAAGTC AGGCTCACCC ACAGCTCCTT CAGCCAGCAG TTCAGTCCAT AGGAATACCA 
GCTAACCTTG GACAAGCTGC TGAGGCTTCA CTTCCCACTG GAGATTCTCT ATACCAGGGA 
TTCCCACCTC GACTGCCACC TCAATACCCA GGAGACTCAA ATATTATTCC CTCATCCACC 
GTGGCTTCTG TTTGCGTCCC TTCTACAGTT CTGTCCCCTC CCTTGCCCAC AGAAGCATTG 
GCTCCACCAG GGTACCTTCC TACAGTTGTG CAGCCATATG TCGAGCCAAG TGTTTTAGTT 
CCTGTGGGTA GTCTAGGAGG ACAGGTTCAA GTACCCCATT CAGCAGTCAC CTTAGCACAG 
CAGGCCCCCT CTGCACCCTC CCAGCCAGCA GCTCTGGAGA CACAAGCAGT TTCCCAGTCC 
AGTCCTCCAG AACCTGTGCC ATCAGCACAG TCACAGACCA CTACATTGGT GTCCTCTATA 
GATAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GGAATGAGAA TGTTCCTTCA 
TCCAGTGGGA GACATGAAGG GAGAACTACA AAACGCCATT ACCGAAAGTC TGTACGGAGT 
CGTTCTCGTC ATGAGAAGAC TGCTCGCCCC AAATTGAGAA TTCTTAATGT GTCAAACAAA 
GGAGACAGAG TGGTGGAATG CCAGTTAGAG ACTCACAACC GGAAAATGGT TACATTCAAA 
TTTGACTTGG ATGGTGACAA TCCTGAGGAG ATAGCATCGA TTATGGTGAA CAATGATTTT 
ATTCTAGCAA CAGAGAGAGA CTCCTTTGTG GATCAGGTGC GAGAAATCAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTTAGTGTG GAACCAGAAG GTGATCAAGG ATCAGAAAGC 
TTACAAACAA AGGATGATTA TGGCTTTTCA GGATCCCAGA AATTGGAGGG AGAGTTCAAG 
CAACCAAATC CTGCATCTTC TATGCCTCAG AGGGCAGGTG TTCCCTCCAG TTCATTAACT 
CAAGTTGTAC ATTCAGCTGG ACGGAGGTTC ATAGTGAGTC CTGTACCAGA GAGTCGGTTA 
CGAGAGTCAG TATTTTTCAC TGCTACATCA AGTGAAATAT CAGACACAAC TGCTGTTGCT 
CCATCCCATG GCCCTGTAAT GAATCTGTCT CATTCTGCTT CATCCGTTAG TTTGCAACAG 
GCATTCTCTG AACTTAGACA TGCTCAAATG TCAGAAGGAC CCAGTACAGC TCCCCCAAAC 
TTCAGCCATG CAGGACCAAC CTTTCCAACT GTACCTGCCA CTATGACTAG CATGGCTGTA 
GTCTTCCCTC CCACGGCAAC AGCTGTGCCT TCAGTATCTG TCCCTGCTAC AAGTAGCCCT 
TTTAATAGTG TTTCTGCACC AGTCATTCAA TCTGTGGTCA CTATGCCTAC TGAAAAGGTA 
ATTGCCGGAG TTGCCTCCAG TGCAGGTGTA TTAACTTCAA GTTCTTTTCC TGTACCACAG 
GTGTCTGGAC CACCAGTAAT GACCAGTGTG GTTTCCAGCA TTACTATTCC TGCAGCCGTG 
TCATTGTCTA CTGCAAGTGA ATCAGTTCAG GCCCCACCAG TTGGGGGAGT TATGTCTAGT 
GCTGGCACTT TTCCTCCTGC AGCGGGATTG GTAGTTCCAG TTCCTGGAAT AGCCAGTACA 
GTTCCCCCAG GTGCTATCAC ACCAGCTATG ACTTCTCAGC CCACCCCAAT TGCAACAAAT 
ATAACTGGGG CAGCCACCCT GACTTCCACA TCTGCCACCA CTCCGTTCCC AAGCATGGCT 
TCACAACCAC CTCTTCAAAT CAGTAGCAGC AGCTCTGCTC CCACTGTAGC AGAAACTGTG 
GTAGTCAGTG CACTCCATCC CAAACACGAT GGCACACATT TACCAGACAA GCCACCCCTT 
AGCAGTGCAG CAGGGCTCTC CCTGCCCCTC TCAGTAGCTC CATCTTCCTC TGGAGCTGGG 
ATCTCTGATT CAGCTAGTCA GCCCAGTGTG CTGCATCCTT TACAGGTCCC ATCCGCTGTG 
GCTTCTCCTC CAGTACTTTC ACAGGCAGCA GGGACCGTCT CCACCCCGGT GTTACCTCAG 
GTACCTTTAC CTGGTATCCC GCCCCTAGCA CAGCCTATTA CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA ACCTCAACCA GCTTCACTTC CCAACCAGCC CCACACTCAT 
TGCCCTGAAG TAGATACTGA TACACAACCC AAAGCCCCTG GCATTGATGA TATAAAGACT 
CTGGAAGAAA AACTGCGGTC TCTCTTCAGT GAACACAGTT CTGTTGGAGC CCAGCATGCC 
CCTGTCTCCC TGGAGACATC ATTGGTAGTG GAGACTACAG TCATGCCAGG CATCCCAACC 
ACCGCTGTGG CACCAAGCAA GCTCATGACT TCCACCACGA GCACTTGCTT ACCACCAAGC 
AGTTTGCCTC TAGGAACAAC TACTGGTTTG TCAGCTGTTA CACCAGTGGT CACACCCGGG 
CAGGCTGCCA CCCCAGTCAG CTATGTTCCA GCCCCCACCA GCACTGCAGT AGGGGTGAAA 
CCTGGAACCC CTCCCTCAAA GCCACCTATC GCCAGGGTAC CGGTACAACC AGTAGGTACT 
GAACTTCAAT CTGGTACCCC ACCCAGCGAG CAGCTGCCGC CTTTTCCAGG ACCTTCCCTA 
ACACAGCCCA CATCAGTTGT GACTCCAACA TCAGTTGCTG GAGCAGTGCC AGTGGTAGTG 
CCAGCTCAGA AAGCTTCAAG CATAGAAGAT AATACCATGA TGACCAACTC AGAGGCTAGC 
GTTCTTAAAA TGGGCCGGTT TCAGGTATCA GTTGCAGTAG ATGATTCCTT GAAGGAAAAT 
AGAAGCAAGT CGGAAGAAAT CAAGTCTGTA CACTTTGAAT CAAGTACTTC AGAGTCCTCA 
GTGCTGTCCA GTAGTAGCCC AGAGAATACA CTGGTGAAGC AGGAGCCTGG TGGAGTCACC 
ACAGCTCTAT GTAGCCCCTC TTTGGATATG GTTGACGGTG TTCCTTCTCA AAAAACCTCT 
GCTACCCAGA CAAAGCCCGA AGCTGAGCAG CCTGCCACAG TGGTTGGCCG ATTCTCTGTG 
TCAAAAACTG AAGATAAGGT CACCCCTGTG GTGGAGGGAG AGCCACCTGT AGTACGCCCT 
TTATTGGATT CTGAACAGGC CGTTCCTTCT GCTGTAGCAC CAAAGAAAGA AATGCCTGAA 
TCGCCAGAGC CAAGCCATCT TAATGGGCCA TCTTGTGACC TAGAAGCTGC CTTTCTCAGT 
AGGAATATGG ATGATGGTTC AGGTAGCCCC CATTCTACTC ACCATTTGGG CTCAAAAGGC 
AGCCTTCCCA TTCAGAGCCT TAGCCAGAGC CTTAGCAATT CATTCAACTC CTCTTACATG 
AGCAGTGACA ATGAGTCAGA CATTGAAGAT GAGGACTTGA AGTCAGAACT ACGTCGGCTA 
CGAGAAAAGC ACCTTAAAGA GATCCAGGAC CTACAGAGTC GCCAGAAACA TGAAATCGAG 
TCTTTATATA CCAAATTGGG CAAAGTCCCT CCTGCAGTCA TCATTCCCCC AGCTGCTCCA 
CTCTCAGGGA GAAGAAGACG ACCCACTAAA AGCAAAGGCA GCAAATCTAG CCGTAGTAGT 
TCTTTAGGAA ATAAGAGCCC CCAACTTTCA GGTAACCTGT CTGCTCAGAG TGCAGCTTCA 
GTCTTGCACC CCCAGCAGAC GCTCCACCCT CCAGGCAGCA TCCCAGAGAC TGGGCAGAAT 
CAGCTGTTAC AGCCCCTTAA ACCATCTCCC TCCAGTGAGA ACCTCTATTC AGCTTTTACC 
AGTGATGGTG CCATTTCAGT ACCAAGCCTT TCTGCTCCAG GCCAAGGGAC CAGCAGTACG 
AATACAGTGG GAGGAGCAGT CAATAGCCAA GCCCCACAAG CCCAGCCGCC CAGTAGAAAG 
GGCACATTTA CAGATGATCT ACACAAGTTG GTAGACAACT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GGAGAGGAAG CAAGGGGCAC ATGAACTATG AGGGTCCTGG AATGGCAAGG 
AAATTTTCAG CACCTGGACA GCTCTGCATC TCCATGACCT CTAATCTGGG TGGTTCTGCC 
CCCATTTCTG CAGCATCAGC TACCTCTTTA GGTCACTTCA CCAAGGCTAT GTGCCCCCCA 
CAGCAGTACA GCTTTCCATC TGCCCCTTTC AGCACTCAGT GGAGTGGGAC AAGTGGCCCA 
GCACCGCCAC CCCTCAGCCA GTTCCAGCCT GTAGGGACTT CGTCCTTGCA GAGTTTCAAC 
ATCAGCAATT TGCAAAAATC CATCAGCAAT CCTCCAGGTT CAAACCTGAG GACAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_012550822.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012406276.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

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CloneID	OSa133595
Clone ID Related Accession (Same CDS sequence)	XM_012550822.1
Accession Version	XM_012550822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003843628.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG AGATGTACGA GGAAAAATAT GATGAGTCCG TTGACGTGTA TGCTTTTGGA 
ATGTGCATGC TTGAGATGGC CACATCCGAA TACCCTTATT CTGAGTGCCA AAATGCTGCG 
CAGATCTACC GTCGAGTGAC CAGTGGAGTA AAGCCAGCCA GCTTTGACAA AGTAGCAATT 
CCTGAAGTAA AGGAAATTAT TGAAGGATGC ATACGCCAAA ACAAAGATGA AAGATATGCT 
ATCAAAGACC TTTTGAACCA TGCCTTCTTC CAGGAGGAAA CAGGAGTACG AGTAGAACTA 
GCTGAAGAAG ATGATGGTGA AAAGATAGCC ATTAAGCTAT GGTTACGCAT TGAAGATATT 
AAGAAATTGA AGGGGAAATA CAAAGACAAT GAAGCAATTG AATTTTCCTT TGATTTGGAG 
AGAGATGTCC CAGAAGATGT AGCTCAAGAA ATGGTAGAAT CAGGGTATGT TTGTGAAGGA 
GATCACAAGA CCATGGCCAA AGCCATCAAA GACAGAGTGT CCCTTATAAA GAGAAAACGA 
GAGCAGCGAC AGTTGGTAAG GGAAGAACAG GAGAAAAGAA AGCAAGAAGA GATCAGTTTA 
AAGGAGCAAG TTGAACAGCC AACAACCATT TCTCAGGCTG GGACCAAATT GATTCCTTCT 
ACCCCTGGCA CAGTTACTGT CTCTACCACA TCAGCCTCAG TTTCCACCCA AGTGGAGCCA 
GAAGAACCAG AGGCAGATCA ACACCAACAA CTGCAGTATC AACAACCTAG TCTCTCCGTG 
TTGTCTGATG GAACAGTTGA CAGTGGTCAG GGGTCTTCAG TCTTCACAGA ATCTCGAGTA 
AGCAGCCAGC AGACTGTCTC ATATGGGTCT CAACATGAAC AGGCACTTTC AACAGGCACG 
ATCCCAAGCT ATACAGCTTC TGTTGTTCAA GCACAATCCC AGCCTCATGG TGTATATCCT 
TCCTCCACCG TGACACCGGC ACAGAGCCAG AGTCATCTGG CTTCAAGTAA CTTAACTGGG 
ATTCCATCTT CCCAGACAGC ACAGCTTGCT CAGCAGGGTA TACAACAAGC AGCCCCTCCT 
CAGCAGACAG GCCAGTATCT TCTTCCCCAG TCAGCAGCAT CTGGTGTGCC TACAACTGCA 
CAGCCTGTGA CGCAGCCCCA GCTTCCACAG GTTCTGCCAA TGCCTCAAGT ATCAGTTGGG 
AAGCAGGGAT TCCCACCTCG ACTGCCACCT CAATACCCAG GAGACTCAAA TATTATTCCC 
TCATCCACCG TGGCTTCTGT TTGCGTCCCT TCTACAGTTC TGTCCCCTCC CTTGCCCACA 
GAAGCATTGG CTCCACCAGG GTACCTTCCT ACAGTTGTGC AGCCATATGT CGAGCCAAGT 
GTTTTAGTTC CTGTGGGTAG TCTAGGAGGA CAGGTTCAAG TACCCCATTC AGCAGTCACC 
TTAGCACAGC AGGCCCCCTC TGCACCCTCC CAGCCAGCAG CTCTGGAGAC ACAAGCAGTT 
TCCCAGTCCA GTCCTCCAGA ACCTGTGCCA TCAGCACAGT CACAGACCAC TACATTGGTG 
TCCTCTATAG ATAGTGCACA TTCAGATGTT GCTTCAGGTA TGAGTGATGG GAATGAGAAT 
GTTCCTTCAT CCAGTGGGAG ACATGAAGGG AGAACTACAA AACGCCATTA CCGAAAGTCT 
GTACGGAGTC GTTCTCGTCA TGAGAAGACT GCTCGCCCCA AATTGAGAAT TCTTAATGTG 
TCAAACAAAG GAGACAGAGT GGTGGAATGC CAGTTAGAGA CTCACAACCG GAAAATGGTT 
ACATTCAAAT TTGACTTGGA TGGTGACAAT CCTGAGGAGA TAGCATCGAT TATGGTGAAC 
AATGATTTTA TTCTAGCAAC AGAGAGAGAC TCCTTTGTGG ATCAGGTGCG AGAAATCATT 
GAAAAAGCTG ATGAAATGCT CAGTGAGGAT GTTAGTGTGG AACCAGAAGG TGATCAAGGA 
TCAGAAAGCT TACAAACAAA GGATGATTAT GGCTTTTCAG GATCCCAGAA ATTGGAGGGA 
GAGTTCAAGC AACCAAATCC TGCATCTTCT ATGCCTCAGA GGGCAGGTGT TCCCTCCAGT 
TCATTAACTC AAGTTGTACA TTCAGCTGGA CGGAGGTTCA TAGTGAGTCC TGTACCAGAG 
AGTCGGTTAC GAGAGTCAGT ATTTTTCACT GCTACATCAA GTGAAATATC AGACACAACT 
GCTGTTGCTC CATCCCATGG CCCTGTAATG AATCTGTCTC ATTCTGCTTC ATCCGTTAGT 
TTGCAACAGG CATTCTCTGA ACTTAGACAT GCTCAAATGT CAGAAGGACC CAGTACAGCT 
CCCCCAAACT TCAGCCATGC AGGACCAACC TTTCCAACTG TACCTGCCAC TATGACTAGC 
ATGGCTGTAG TCTTCCCTCC CACGGCAACA GCTGTGCCTT CAGTATCTGT CCCTGCTACA 
AGTAGCCCTT TTAATAGTGT TTCTGCACCA GTCATTCAAT CTGTGGTCAC TATGCCTACT 
GAAAAGGTAA TTGCCGGAGT TGCCTCCAGT GCAGGTGTAT TAACTTCAAG TTCTTTTCCT 
GTACCACAGG TGTCTGGACC ACCAGTAATG ACCAGTGTGG TTTCCAGCAT TACTATTCCT 
GCAGCCGTGT CATTGTCTAC TGCAAGTGAA TCAGTTCAGG CCCCACCAGT TGGGGGAGTT 
ATGTCTAGTG CTGGCACTTT TCCTCCTGCA GCGGGATTGG TAGTTCCAGT TCCTGGAATA 
GCCAGTACAG TTCCCCCAGG TGCTATCACA CCAGCTATGA CTTCTCAGCC CACCCCAATT 
GCAACAAATA TAACTGGGGC AGCCACCCTG ACTTCCACAT CTGCCACCAC TCCGTTCCCA 
AGCATGGCTT CACAACCACC TCTTCAAATC AGTAGCAGCA GCTCTGCTCC CACTGTAGCA 
GAAACTGTGG TAGTCAGTGC ACTCCATCCC AAACACGATG GCACACATTT ACCAGACAAG 
CCACCCCTTA GCAGTGCAGC AGGGCTCTCC CTGCCCCTCT CAGTAGCTCC ATCTTCCTCT 
GGAGCTGGGA TCTCTGATTC AGCTAGTCAG CCCAGTGTGC TGCATCCTTT ACAGGTCCCA 
TCCGCTGTGG CTTCTCCTCC AGTACTTTCA CAGGCAGCAG GGACCGTCTC CACCCCGGTG 
TTACCTCAGG TACCTTTACC TGGTATCCCG CCCCTAGCAC AGCCTATTAC CAATGTGCCT 
GCTGTACAGC AGACACTAAT TCATAGTCAA CCTCAACCAG CTTCACTTCC CAACCAGCCC 
CACACTCATT GCCCTGAAGT AGATACTGAT ACACAACCCA AAGCCCCTGG CATTGATGAT 
ATAAAGACTC TGGAAGAAAA ACTGCGGTCT CTCTTCAGTG AACACAGTTC TGTTGGAGCC 
CAGCATGCCC CTGTCTCCCT GGAGACATCA TTGGTAGTGG AGACTACAGT CATGCCAGGC 
ATCCCAACCA CCGCTGTGGC ACCAAGCAAG CTCATGACTT CCACCACGAG CACTTGCTTA 
CCACCAAGCA GTTTGCCTCT AGGAACAACT ACTGGTTTGT CAGCTGTTAC ACCAGTGGTC 
ACACCCGGGC AGGCTGCCAC CCCAGTCAGC TATGTTCCAG CCCCCACCAG CACTGCAGTA 
GGGGTGAAAC CTGGAACCCC TCCCTCAAAG CCACCTATCG CCAGGGTACC GGTACAACCA 
GTAGGTACTG AACTTCAATC TGGTACCCCA CCCAGCGAGC AGCTGCCGCC TTTTCCAGGA 
CCTTCCCTAA CACAGTCTCA GCAACCTCTA GAAGATCTTG ATGCTCAGTT AAGAAGAGCC 
CTTAGTCCAG AGACTGTCCC AGTCACATCT TCAGTTGCTC CCACATCAGT TGTGACTCCA 
ACATCAGTTG CTGGAGCAGT GCCAGTGGTA GTGCCAGCTC AGAAAGCAGC TTCAAGCATA 
GAAGATAATA CCATGATGAC CAACTCAGAG GCTAGCGTTC TTAAAATGGG CCGGTTTCAG 
GTATCAGTTG CAGTAGATGA TTCCTTGAAG GAAAATAGAA GCAAGTCGGA AGAAATCAAG 
TCTGTACACT TTGAATCAAG TACTTCAGAG TCCTCAGTGC TGTCCAGTAG TAGCCCAGAG 
AATACACTGG TGAAGCAGGA GCCTGGTGGA GTCACCACAG CTCTATGTAG CCCCTCTTTG 
GATATGGTTG ACGGTGTTCC TTCTCAAAAA ACCTCTGCTA CCCAGACAAA GCCCGAAGCT 
GAGCAGCCTG CCACAGTGGT TGGCCGATTC TCTGTGTCAA AAACTGAAGA TAAGGTCACC 
CCTGTGGTGG AGGGAGAGCC ACCTGTAGTA CGCCCTTTAT TGGATTCTGA ACAGGCCGTT 
CCTTCTGCTG TAGCACCAAA GAAAGAAATG CCTGAATCGC CAGAGCCAAG CCATCTTAAT 
GGGCCATCTT GTGACCTAGA AGCTGCCTTT CTCAGTAGGA ATATGGATGA TGGTTCAGGT 
AGCCCCCATT CTACTCACCA TTTGGGCTCA AAAGGCAGCC TTCCCATTCA GAGCCTTAGC 
CAGAGCCTTA GCAATTCATT CAACTCCTCT TACATGAGCA GTGACAATGA GTCAGACATT 
GAAGATGAGG ACTTGAAGTC AGAACTACGT CGGCTACGAG AAAAGCACCT TAAAGAGATC 
CAGGACCTAC AGAGTCGCCA GAAACATGAA ATCGAGTCTT TATATACCAA ATTGGGCAAA 
GTCCCTCCTG CAGTCATCAT TCCCCCAGCT GCTCCACTCT CAGGGAGAAG AAGACGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGCCGT AGTAGTTCTT TAGGAAATAA GAGCCCCCAA 
CTTTCAGGTA ACCTGTCTGC TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACGCTC 
CACCCTCCAG GCAGCATCCC AGAGACTGGG CAGAATCAGC TGTTACAGCC CCTTAAACCA 
TCTCCCTCCA GTGAGAACCT CTATTCAGCT TTTACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGCCA AGGGACCAGC AGTACGAATA CAGTGGGAGG AGCAGTCAAT 
AGCCAAGCCC CACAAGCCCA GCCGCCCAGT AGAAAGGGCA CATTTACAGA TGATCTACAC 
AAGTTGGTAG ACAACTGGGC CCGAGATGCC ATGAATCTCT CAGGCAGGAG AGGAAGCAAG 
GGGCACATGA ACTATGAGGG TCCTGGAATG GCAAGGAAAT TTTCAGCACC TGGACAGCTC 
TGCATCTCCA TGACCTCTAA TCTGGGTGGT TCTGCCCCCA TTTCTGCAGC ATCAGCTACC 
TCTTTAGGTC ACTTCACCAA GGCTATGTGC CCCCCACAGC AGTACAGCTT TCCATCTGCC 
CCTTTCAGCA CTCAGTGGAG TGGGACAAGT GGCCCAGCAC CGCCACCCCT CAGCCAGTTC 
CAGCCTGTAG GGACTTCGTC CTTGCAGAGT TTCAACATCA GCAATTTGCA AAAATCCATC 
AGCAATCCTC CAGGTTCAAA CCTGAGGACA ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012406276.1
CDS	24..5579
Translation	MAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_012550822.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012550822.1

ORF Insert Sequence:

ATGGCCCCTG AGATGTACGA GGAAAAATAT GATGAGTCCG TTGACGTGTA TGCTTTTGGA 
ATGTGCATGC TTGAGATGGC CACATCCGAA TACCCTTATT CTGAGTGCCA AAATGCTGCG 
CAGATCTACC GTCGAGTGAC CAGTGGAGTA AAGCCAGCCA GCTTTGACAA AGTAGCAATT 
CCTGAAGTAA AGGAAATTAT TGAAGGATGC ATACGCCAAA ACAAAGATGA AAGATATGCT 
ATCAAAGACC TTTTGAACCA TGCCTTCTTC CAGGAGGAAA CAGGAGTACG AGTAGAACTA 
GCTGAAGAAG ATGATGGTGA AAAGATAGCC ATTAAGCTAT GGTTACGCAT TGAAGATATT 
AAGAAATTGA AGGGGAAATA CAAAGACAAT GAAGCAATTG AATTTTCCTT TGATTTGGAG 
AGAGATGTCC CAGAAGATGT AGCTCAAGAA ATGGTAGAAT CAGGGTATGT TTGTGAAGGA 
GATCACAAGA CCATGGCCAA AGCCATCAAA GACAGAGTGT CCCTTATAAA GAGAAAACGA 
GAGCAGCGAC AGTTGGTAAG GGAAGAACAG GAGAAAAGAA AGCAAGAAGA GATCAGTTTA 
AAGGAGCAAG TTGAACAGCC AACAACCATT TCTCAGGCTG GGACCAAATT GATTCCTTCT 
ACCCCTGGCA CAGTTACTGT CTCTACCACA TCAGCCTCAG TTTCCACCCA AGTGGAGCCA 
GAAGAACCAG AGGCAGATCA ACACCAACAA CTGCAGTATC AACAACCTAG TCTCTCCGTG 
TTGTCTGATG GAACAGTTGA CAGTGGTCAG GGGTCTTCAG TCTTCACAGA ATCTCGAGTA 
AGCAGCCAGC AGACTGTCTC ATATGGGTCT CAACATGAAC AGGCACTTTC AACAGGCACG 
ATCCCAAGCT ATACAGCTTC TGTTGTTCAA GCACAATCCC AGCCTCATGG TGTATATCCT 
TCCTCCACCG TGACACCGGC ACAGAGCCAG AGTCATCTGG CTTCAAGTAA CTTAACTGGG 
ATTCCATCTT CCCAGACAGC ACAGCTTGCT CAGCAGGGTA TACAACAAGC AGCCCCTCCT 
CAGCAGACAG GCCAGTATCT TCTTCCCCAG TCAGCAGCAT CTGGTGTGCC TACAACTGCA 
CAGCCTGTGA CGCAGCCCCA GCTTCCACAG GTTCTGCCAA TGCCTCAAGT ATCAGTTGGG 
AAGCAGGGAT TCCCACCTCG ACTGCCACCT CAATACCCAG GAGACTCAAA TATTATTCCC 
TCATCCACCG TGGCTTCTGT TTGCGTCCCT TCTACAGTTC TGTCCCCTCC CTTGCCCACA 
GAAGCATTGG CTCCACCAGG GTACCTTCCT ACAGTTGTGC AGCCATATGT CGAGCCAAGT 
GTTTTAGTTC CTGTGGGTAG TCTAGGAGGA CAGGTTCAAG TACCCCATTC AGCAGTCACC 
TTAGCACAGC AGGCCCCCTC TGCACCCTCC CAGCCAGCAG CTCTGGAGAC ACAAGCAGTT 
TCCCAGTCCA GTCCTCCAGA ACCTGTGCCA TCAGCACAGT CACAGACCAC TACATTGGTG 
TCCTCTATAG ATAGTGCACA TTCAGATGTT GCTTCAGGTA TGAGTGATGG GAATGAGAAT 
GTTCCTTCAT CCAGTGGGAG ACATGAAGGG AGAACTACAA AACGCCATTA CCGAAAGTCT 
GTACGGAGTC GTTCTCGTCA TGAGAAGACT GCTCGCCCCA AATTGAGAAT TCTTAATGTG 
TCAAACAAAG GAGACAGAGT GGTGGAATGC CAGTTAGAGA CTCACAACCG GAAAATGGTT 
ACATTCAAAT TTGACTTGGA TGGTGACAAT CCTGAGGAGA TAGCATCGAT TATGGTGAAC 
AATGATTTTA TTCTAGCAAC AGAGAGAGAC TCCTTTGTGG ATCAGGTGCG AGAAATCATT 
GAAAAAGCTG ATGAAATGCT CAGTGAGGAT GTTAGTGTGG AACCAGAAGG TGATCAAGGA 
TCAGAAAGCT TACAAACAAA GGATGATTAT GGCTTTTCAG GATCCCAGAA ATTGGAGGGA 
GAGTTCAAGC AACCAAATCC TGCATCTTCT ATGCCTCAGA GGGCAGGTGT TCCCTCCAGT 
TCATTAACTC AAGTTGTACA TTCAGCTGGA CGGAGGTTCA TAGTGAGTCC TGTACCAGAG 
AGTCGGTTAC GAGAGTCAGT ATTTTTCACT GCTACATCAA GTGAAATATC AGACACAACT 
GCTGTTGCTC CATCCCATGG CCCTGTAATG AATCTGTCTC ATTCTGCTTC ATCCGTTAGT 
TTGCAACAGG CATTCTCTGA ACTTAGACAT GCTCAAATGT CAGAAGGACC CAGTACAGCT 
CCCCCAAACT TCAGCCATGC AGGACCAACC TTTCCAACTG TACCTGCCAC TATGACTAGC 
ATGGCTGTAG TCTTCCCTCC CACGGCAACA GCTGTGCCTT CAGTATCTGT CCCTGCTACA 
AGTAGCCCTT TTAATAGTGT TTCTGCACCA GTCATTCAAT CTGTGGTCAC TATGCCTACT 
GAAAAGGTAA TTGCCGGAGT TGCCTCCAGT GCAGGTGTAT TAACTTCAAG TTCTTTTCCT 
GTACCACAGG TGTCTGGACC ACCAGTAATG ACCAGTGTGG TTTCCAGCAT TACTATTCCT 
GCAGCCGTGT CATTGTCTAC TGCAAGTGAA TCAGTTCAGG CCCCACCAGT TGGGGGAGTT 
ATGTCTAGTG CTGGCACTTT TCCTCCTGCA GCGGGATTGG TAGTTCCAGT TCCTGGAATA 
GCCAGTACAG TTCCCCCAGG TGCTATCACA CCAGCTATGA CTTCTCAGCC CACCCCAATT 
GCAACAAATA TAACTGGGGC AGCCACCCTG ACTTCCACAT CTGCCACCAC TCCGTTCCCA 
AGCATGGCTT CACAACCACC TCTTCAAATC AGTAGCAGCA GCTCTGCTCC CACTGTAGCA 
GAAACTGTGG TAGTCAGTGC ACTCCATCCC AAACACGATG GCACACATTT ACCAGACAAG 
CCACCCCTTA GCAGTGCAGC AGGGCTCTCC CTGCCCCTCT CAGTAGCTCC ATCTTCCTCT 
GGAGCTGGGA TCTCTGATTC AGCTAGTCAG CCCAGTGTGC TGCATCCTTT ACAGGTCCCA 
TCCGCTGTGG CTTCTCCTCC AGTACTTTCA CAGGCAGCAG GGACCGTCTC CACCCCGGTG 
TTACCTCAGG TACCTTTACC TGGTATCCCG CCCCTAGCAC AGCCTATTAC CAATGTGCCT 
GCTGTACAGC AGACACTAAT TCATAGTCAA CCTCAACCAG CTTCACTTCC CAACCAGCCC 
CACACTCATT GCCCTGAAGT AGATACTGAT ACACAACCCA AAGCCCCTGG CATTGATGAT 
ATAAAGACTC TGGAAGAAAA ACTGCGGTCT CTCTTCAGTG AACACAGTTC TGTTGGAGCC 
CAGCATGCCC CTGTCTCCCT GGAGACATCA TTGGTAGTGG AGACTACAGT CATGCCAGGC 
ATCCCAACCA CCGCTGTGGC ACCAAGCAAG CTCATGACTT CCACCACGAG CACTTGCTTA 
CCACCAAGCA GTTTGCCTCT AGGAACAACT ACTGGTTTGT CAGCTGTTAC ACCAGTGGTC 
ACACCCGGGC AGGCTGCCAC CCCAGTCAGC TATGTTCCAG CCCCCACCAG CACTGCAGTA 
GGGGTGAAAC CTGGAACCCC TCCCTCAAAG CCACCTATCG CCAGGGTACC GGTACAACCA 
GTAGGTACTG AACTTCAATC TGGTACCCCA CCCAGCGAGC AGCTGCCGCC TTTTCCAGGA 
CCTTCCCTAA CACAGTCTCA GCAACCTCTA GAAGATCTTG ATGCTCAGTT AAGAAGAGCC 
CTTAGTCCAG AGACTGTCCC AGTCACATCT TCAGTTGCTC CCACATCAGT TGTGACTCCA 
ACATCAGTTG CTGGAGCAGT GCCAGTGGTA GTGCCAGCTC AGAAAGCAGC TTCAAGCATA 
GAAGATAATA CCATGATGAC CAACTCAGAG GCTAGCGTTC TTAAAATGGG CCGGTTTCAG 
GTATCAGTTG CAGTAGATGA TTCCTTGAAG GAAAATAGAA GCAAGTCGGA AGAAATCAAG 
TCTGTACACT TTGAATCAAG TACTTCAGAG TCCTCAGTGC TGTCCAGTAG TAGCCCAGAG 
AATACACTGG TGAAGCAGGA GCCTGGTGGA GTCACCACAG CTCTATGTAG CCCCTCTTTG 
GATATGGTTG ACGGTGTTCC TTCTCAAAAA ACCTCTGCTA CCCAGACAAA GCCCGAAGCT 
GAGCAGCCTG CCACAGTGGT TGGCCGATTC TCTGTGTCAA AAACTGAAGA TAAGGTCACC 
CCTGTGGTGG AGGGAGAGCC ACCTGTAGTA CGCCCTTTAT TGGATTCTGA ACAGGCCGTT 
CCTTCTGCTG TAGCACCAAA GAAAGAAATG CCTGAATCGC CAGAGCCAAG CCATCTTAAT 
GGGCCATCTT GTGACCTAGA AGCTGCCTTT CTCAGTAGGA ATATGGATGA TGGTTCAGGT 
AGCCCCCATT CTACTCACCA TTTGGGCTCA AAAGGCAGCC TTCCCATTCA GAGCCTTAGC 
CAGAGCCTTA GCAATTCATT CAACTCCTCT TACATGAGCA GTGACAATGA GTCAGACATT 
GAAGATGAGG ACTTGAAGTC AGAACTACGT CGGCTACGAG AAAAGCACCT TAAAGAGATC 
CAGGACCTAC AGAGTCGCCA GAAACATGAA ATCGAGTCTT TATATACCAA ATTGGGCAAA 
GTCCCTCCTG CAGTCATCAT TCCCCCAGCT GCTCCACTCT CAGGGAGAAG AAGACGACCC 
ACTAAAAGCA AAGGCAGCAA ATCTAGCCGT AGTAGTTCTT TAGGAAATAA GAGCCCCCAA 
CTTTCAGGTA ACCTGTCTGC TCAGAGTGCA GCTTCAGTCT TGCACCCCCA GCAGACGCTC 
CACCCTCCAG GCAGCATCCC AGAGACTGGG CAGAATCAGC TGTTACAGCC CCTTAAACCA 
TCTCCCTCCA GTGAGAACCT CTATTCAGCT TTTACCAGTG ATGGTGCCAT TTCAGTACCA 
AGCCTTTCTG CTCCAGGCCA AGGGACCAGC AGTACGAATA CAGTGGGAGG AGCAGTCAAT 
AGCCAAGCCC CACAAGCCCA GCCGCCCAGT AGAAAGGGCA CATTTACAGA TGATCTACAC 
AAGTTGGTAG ACAACTGGGC CCGAGATGCC ATGAATCTCT CAGGCAGGAG AGGAAGCAAG 
GGGCACATGA ACTATGAGGG TCCTGGAATG GCAAGGAAAT TTTCAGCACC TGGACAGCTC 
TGCATCTCCA TGACCTCTAA TCTGGGTGGT TCTGCCCCCA TTTCTGCAGC ATCAGCTACC 
TCTTTAGGTC ACTTCACCAA GGCTATGTGC CCCCCACAGC AGTACAGCTT TCCATCTGCC 
CCTTTCAGCA CTCAGTGGAG TGGGACAAGT GGCCCAGCAC CGCCACCCCT CAGCCAGTTC 
CAGCCTGTAG GGACTTCGTC CTTGCAGAGT TTCAACATCA GCAATTTGCA AAAATCCATC 
AGCAATCCTC CAGGTTCAAA CCTGAGGACA ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_012550823.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012406277.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSa133596
Clone ID Related Accession (Same CDS sequence)	XM_012550823.1
Accession Version	XM_012550823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003843628.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG AGATGTACGA GGAAAAATAT GATGAGTCCG TTGACGTGTA TGCTTTTGGA 
ATGTGCATGC TTGAGATGGC CACATCCGAA TACCCTTATT CTGAGTGCCA AAATGCTGCG 
CAGATCTACC GTCGAGTGAC CAGTGGAGTA AAGCCAGCCA GCTTTGACAA AGTAGCAATT 
CCTGAAGTAA AGGAAATTAT TGAAGGATGC ATACGCCAAA ACAAAGATGA AAGATATGCT 
ATCAAAGACC TTTTGAACCA TGCCTTCTTC CAGGAGGAAA CAGGAGTACG AGTAGAACTA 
GCTGAAGAAG ATGATGGTGA AAAGATAGCC ATTAAGCTAT GGTTACGCAT TGAAGATATT 
AAGAAATTGA AGGGGAAATA CAAAGACAAT GAAGCAATTG AATTTTCCTT TGATTTGGAG 
AGAGATGTCC CAGAAGATGT AGCTCAAGAA ATGGTAGAAT CAGGGTATGT TTGTGAAGGA 
GATCACAAGA CCATGGCCAA AGCCATCAAA GACAGAGTGT CCCTTATAAA GAGAAAACGA 
GAGCAGCGAC AGTTGGTAAG GGAAGAACAG GAGAAAAGAA AGCAAGAAGA GATCAGTTTA 
AAGGAGCAAG TTGAACAGCC AACAACCATT TCTCAGGCTG GGACCAAATT GATTCCTTCT 
ACCCCTGGCA CAGTTACTGT CTCTACCACA TCAGCCTCAG TTTCCACCCA AGTGGAGCCA 
GAAGAACCAG AGGCAGATCA ACACCAACAA CTGCAGTATC AACAACCTAG TCTCTCCGTG 
TTGTCTGATG GAACAGTTGA CAGTGGTCAG GGGTCTTCAG TCTTCACAGA ATCTCGAGTA 
AGCAGCCAGC AGACTGTCTC ATATGGGTCT CAACATGAAC AGGCACTTTC AACAGGCACG 
ATCCCAAGCT ATACAGCTTC TGTTGTTCAA GCACAATCCC AGCCTCATGG TGTATATCCT 
TCCTCCACCG TGACACCGGC ACAGAGCCAG AGTCATCTGG CTTCAAGTAA CTTAACTGGG 
ATTCCATCTT CCCAGACAGC ACAGCTTGCT CAGCAGGGTA TACAACAAGC AGCCCCTCCT 
CAGCAGACAG GCCAGTATCT TCTTCCCCAG TCAGCAGCAT CTGGTGTGCC TACAACTGCA 
CAGCCTGTGA CGCAGCCCCA GCTTCCACAG GTTCTGCCAA TGCCTCAAGT ATCAGTTGGG 
AAGCAGACAC AAGCAGTTTC CCAGTCCAGT CCTCCAGAAC CTGTGCCATC AGCACAGTCA 
CAGACCACTA CATTGGTGTC CTCTATAGAT AGTGCACATT CAGATGTTGC TTCAGGTATG 
AGTGATGGGA ATGAGAATGT TCCTTCATCC AGTGGGAGAC ATGAAGGGAG AACTACAAAA 
CGCCATTACC GAAAGTCTGT ACGGAGTCGT TCTCGTCATG AGAAGACTGC TCGCCCCAAA 
TTGAGAATTC TTAATGTGTC AAACAAAGGA GACAGAGTGG TGGAATGCCA GTTAGAGACT 
CACAACCGGA AAATGGTTAC ATTCAAATTT GACTTGGATG GTGACAATCC TGAGGAGATA 
GCATCGATTA TGGTGAACAA TGATTTTATT CTAGCAACAG AGAGAGACTC CTTTGTGGAT 
CAGGTGCGAG AAATCATTGA AAAAGCTGAT GAAATGCTCA GTGAGGATGT TAGTGTGGAA 
CCAGAAGGTG ATCAAGGATC AGAAAGCTTA CAAACAAAGG ATGATTATGG CTTTTCAGGA 
TCCCAGAAAT TGGAGGGAGA GTTCAAGCAA CCAAATCCTG CATCTTCTAT GCCTCAGAGG 
GCAGGTGTTC CCTCCAGTTC ATTAACTCAA GTTGTACATT CAGCTGGACG GAGGTTCATA 
GTGAGTCCTG TACCAGAGAG TCGGTTACGA GAGTCAGTAT TTTTCACTGC TACATCAAGT 
GAAATATCAG ACACAACTGC TGTTGCTCCA TCCCATGGCC CTGTAATGAA TCTGTCTCAT 
TCTGCTTCAT CCGTTAGTTT GCAACAGGCA TTCTCTGAAC TTAGACATGC TCAAATGTCA 
GAAGGACCCA GTACAGCTCC CCCAAACTTC AGCCATGCAG GACCAACCTT TCCAACTGTA 
CCTGCCACTA TGACTAGCAT GGCTGTAGTC TTCCCTCCCA CGGCAACAGC TGTGCCTTCA 
GTATCTGTCC CTGCTACAAG TAGCCCTTTT AATAGTGTTT CTGCACCAGT CATTCAATCT 
GTGGTCACTA TGCCTACTGA AAAGGTAATT GCCGGAGTTG CCTCCAGTGC AGGTGTATTA 
ACTTCAAGTT CTTTTCCTGT ACCACAGGTG TCTGGACCAC CAGTAATGAC CAGTGTGGTT 
TCCAGCATTA CTATTCCTGC AGCCGTGTCA TTGTCTACTG CAAGTGAATC AGTTCAGGCC 
CCACCAGTTG GGGGAGTTAT GTCTAGTGCT GGCACTTTTC CTCCTGCAGC GGGATTGGTA 
GTTCCAGTTC CTGGAATAGC CAGTACAGTT CCCCCAGGTG CTATCACACC AGCTATGACT 
TCTCAGCCCA CCCCAATTGC AACAAATATA ACTGGGGCAG CCACCCTGAC TTCCACATCT 
GCCACCACTC CGTTCCCAAG CATGGCTTCA CAACCACCTC TTCAAATCAG TAGCAGCAGC 
TCTGCTCCCA CTGTAGCAGA AACTGTGGTA GTCAGTGCAC TCCATCCCAA ACACGATGGC 
ACACATTTAC CAGACAAGCC ACCCCTTAGC AGTGCAGCAG GGCTCTCCCT GCCCCTCTCA 
GTAGCTCCAT CTTCCTCTGG AGCTGGGATC TCTGATTCAG CTAGTCAGCC CAGTGTGCTG 
CATCCTTTAC AGGTCCCATC CGCTGTGGCT TCTCCTCCAG TACTTTCACA GGCAGCAGGG 
ACCGTCTCCA CCCCGGTGTT ACCTCAGGTA CCTTTACCTG GTATCCCGCC CCTAGCACAG 
CCTATTACCA ATGTGCCTGC TGTACAGCAG ACACTAATTC ATAGTCAACC TCAACCAGCT 
TCACTTCCCA ACCAGCCCCA CACTCATTGC CCTGAAGTAG ATACTGATAC ACAACCCAAA 
GCCCCTGGCA TTGATGATAT AAAGACTCTG GAAGAAAAAC TGCGGTCTCT CTTCAGTGAA 
CACAGTTCTG TTGGAGCCCA GCATGCCCCT GTCTCCCTGG AGACATCATT GGTAGTGGAG 
ACTACAGTCA TGCCAGGCAT CCCAACCACC GCTGTGGCAC CAAGCAAGCT CATGACTTCC 
ACCACGAGCA CTTGCTTACC ACCAAGCAGT TTGCCTCTAG GAACAACTAC TGGTTTGTCA 
GCTGTTACAC CAGTGGTCAC ACCCGGGCAG GCTGCCACCC CAGTCAGCTA TGTTCCAGCC 
CCCACCAGCA CTGCAGTAGG GGTGAAACCT GGAACCCCTC CCTCAAAGCC ACCTATCGCC 
AGGGTACCGG TACAACCAGT AGGTACTGAA CTTCAATCTG GTACCCCACC CAGCGAGCAG 
CTGCCGCCTT TTCCAGGACC TTCCCTAACA CAGTCTCAGC AACCTCTAGA AGATCTTGAT 
GCTCAGTTAA GAAGAGCCCT TAGTCCAGAG ACTGTCCCAG TCACATCTTC AGTTGCTCCC 
ACATCAGTTG TGACTCCAAC ATCAGTTGCT GGAGCAGTGC CAGTGGTAGT GCCAGCTCAG 
AAAGCAGCTT CAAGCATAGA AGATAATACC ATGATGACCA ACTCAGAGGC TAGCGTTCTT 
AAAATGGGCC GGTTTCAGGT ATCAGTTGCA GTAGATGATT CCTTGAAGGA AAATAGAAGC 
AAGTCGGAAG AAATCAAGTC TGTACACTTT GAATCAAGTA CTTCAGAGTC CTCAGTGCTG 
TCCAGTAGTA GCCCAGAGAA TACACTGGTG AAGCAGGAGC CTGGTGGAGT CACCACAGCT 
CTATGTAGCC CCTCTTTGGA TATGGTTGAC GGTGTTCCTT CTCAAAAAAC CTCTGCTACC 
CAGACAAAGC CCGAAGCTGA GCAGCCTGCC ACAGTGGTTG GCCGATTCTC TGTGTCAAAA 
ACTGAAGATA AGGTCACCCC TGTGGTGGAG GGAGAGCCAC CTGTAGTACG CCCTTTATTG 
GATTCTGAAC AGGCCGTTCC TTCTGCTGTA GCACCAAAGA AAGAAATGCC TGAATCGCCA 
GAGCCAAGCC ATCTTAATGG GCCATCTTGT GACCTAGAAG CTGCCTTTCT CAGTAGGAAT 
ATGGATGATG GTTCAGGTAG CCCCCATTCT ACTCACCATT TGGGCTCAAA AGGCAGCCTT 
CCCATTCAGA GCCTTAGCCA GAGCCTTAGC AATTCATTCA ACTCCTCTTA CATGAGCAGT 
GACAATGAGT CAGACATTGA AGATGAGGAC TTGAAGTCAG AACTACGTCG GCTACGAGAA 
AAGCACCTTA AAGAGATCCA GGACCTACAG AGTCGCCAGA AACATGAAAT CGAGTCTTTA 
TATACCAAAT TGGGCAAAGT CCCTCCTGCA GTCATCATTC CCCCAGCTGC TCCACTCTCA 
GGGAGAAGAA GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGCCGTAG TAGTTCTTTA 
GGAAATAAGA GCCCCCAACT TTCAGGTAAC CTGTCTGCTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACGCTCCA CCCTCCAGGC AGCATCCCAG AGACTGGGCA GAATCAGCTG 
TTACAGCCCC TTAAACCATC TCCCTCCAGT GAGAACCTCT ATTCAGCTTT TACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGCCAAG GGACCAGCAG TACGAATACA 
GTGGGAGGAG CAGTCAATAG CCAAGCCCCA CAAGCCCAGC CGCCCAGTAG AAAGGGCACA 
TTTACAGATG ATCTACACAA GTTGGTAGAC AACTGGGCCC GAGATGCCAT GAATCTCTCA 
GGCAGGAGAG GAAGCAAGGG GCACATGAAC TATGAGGGTC CTGGAATGGC AAGGAAATTT 
TCAGCACCTG GACAGCTCTG CATCTCCATG ACCTCTAATC TGGGTGGTTC TGCCCCCATT 
TCTGCAGCAT CAGCTACCTC TTTAGGTCAC TTCACCAAGG CTATGTGCCC CCCACAGCAG 
TACAGCTTTC CATCTGCCCC TTTCAGCACT CAGTGGAGTG GGACAAGTGG CCCAGCACCG 
CCACCCCTCA GCCAGTTCCA GCCTGTAGGG ACTTCGTCCT TGCAGAGTTT CAACATCAGC 
AATTTGCAAA AATCCATCAG CAATCCTCCA GGTTCAAACC TGAGGACAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012406277.1
CDS	24..5297
Translation	MAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQTQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_012550823.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012550823.1

ORF Insert Sequence:

ATGGCCCCTG AGATGTACGA GGAAAAATAT GATGAGTCCG TTGACGTGTA TGCTTTTGGA 
ATGTGCATGC TTGAGATGGC CACATCCGAA TACCCTTATT CTGAGTGCCA AAATGCTGCG 
CAGATCTACC GTCGAGTGAC CAGTGGAGTA AAGCCAGCCA GCTTTGACAA AGTAGCAATT 
CCTGAAGTAA AGGAAATTAT TGAAGGATGC ATACGCCAAA ACAAAGATGA AAGATATGCT 
ATCAAAGACC TTTTGAACCA TGCCTTCTTC CAGGAGGAAA CAGGAGTACG AGTAGAACTA 
GCTGAAGAAG ATGATGGTGA AAAGATAGCC ATTAAGCTAT GGTTACGCAT TGAAGATATT 
AAGAAATTGA AGGGGAAATA CAAAGACAAT GAAGCAATTG AATTTTCCTT TGATTTGGAG 
AGAGATGTCC CAGAAGATGT AGCTCAAGAA ATGGTAGAAT CAGGGTATGT TTGTGAAGGA 
GATCACAAGA CCATGGCCAA AGCCATCAAA GACAGAGTGT CCCTTATAAA GAGAAAACGA 
GAGCAGCGAC AGTTGGTAAG GGAAGAACAG GAGAAAAGAA AGCAAGAAGA GATCAGTTTA 
AAGGAGCAAG TTGAACAGCC AACAACCATT TCTCAGGCTG GGACCAAATT GATTCCTTCT 
ACCCCTGGCA CAGTTACTGT CTCTACCACA TCAGCCTCAG TTTCCACCCA AGTGGAGCCA 
GAAGAACCAG AGGCAGATCA ACACCAACAA CTGCAGTATC AACAACCTAG TCTCTCCGTG 
TTGTCTGATG GAACAGTTGA CAGTGGTCAG GGGTCTTCAG TCTTCACAGA ATCTCGAGTA 
AGCAGCCAGC AGACTGTCTC ATATGGGTCT CAACATGAAC AGGCACTTTC AACAGGCACG 
ATCCCAAGCT ATACAGCTTC TGTTGTTCAA GCACAATCCC AGCCTCATGG TGTATATCCT 
TCCTCCACCG TGACACCGGC ACAGAGCCAG AGTCATCTGG CTTCAAGTAA CTTAACTGGG 
ATTCCATCTT CCCAGACAGC ACAGCTTGCT CAGCAGGGTA TACAACAAGC AGCCCCTCCT 
CAGCAGACAG GCCAGTATCT TCTTCCCCAG TCAGCAGCAT CTGGTGTGCC TACAACTGCA 
CAGCCTGTGA CGCAGCCCCA GCTTCCACAG GTTCTGCCAA TGCCTCAAGT ATCAGTTGGG 
AAGCAGACAC AAGCAGTTTC CCAGTCCAGT CCTCCAGAAC CTGTGCCATC AGCACAGTCA 
CAGACCACTA CATTGGTGTC CTCTATAGAT AGTGCACATT CAGATGTTGC TTCAGGTATG 
AGTGATGGGA ATGAGAATGT TCCTTCATCC AGTGGGAGAC ATGAAGGGAG AACTACAAAA 
CGCCATTACC GAAAGTCTGT ACGGAGTCGT TCTCGTCATG AGAAGACTGC TCGCCCCAAA 
TTGAGAATTC TTAATGTGTC AAACAAAGGA GACAGAGTGG TGGAATGCCA GTTAGAGACT 
CACAACCGGA AAATGGTTAC ATTCAAATTT GACTTGGATG GTGACAATCC TGAGGAGATA 
GCATCGATTA TGGTGAACAA TGATTTTATT CTAGCAACAG AGAGAGACTC CTTTGTGGAT 
CAGGTGCGAG AAATCATTGA AAAAGCTGAT GAAATGCTCA GTGAGGATGT TAGTGTGGAA 
CCAGAAGGTG ATCAAGGATC AGAAAGCTTA CAAACAAAGG ATGATTATGG CTTTTCAGGA 
TCCCAGAAAT TGGAGGGAGA GTTCAAGCAA CCAAATCCTG CATCTTCTAT GCCTCAGAGG 
GCAGGTGTTC CCTCCAGTTC ATTAACTCAA GTTGTACATT CAGCTGGACG GAGGTTCATA 
GTGAGTCCTG TACCAGAGAG TCGGTTACGA GAGTCAGTAT TTTTCACTGC TACATCAAGT 
GAAATATCAG ACACAACTGC TGTTGCTCCA TCCCATGGCC CTGTAATGAA TCTGTCTCAT 
TCTGCTTCAT CCGTTAGTTT GCAACAGGCA TTCTCTGAAC TTAGACATGC TCAAATGTCA 
GAAGGACCCA GTACAGCTCC CCCAAACTTC AGCCATGCAG GACCAACCTT TCCAACTGTA 
CCTGCCACTA TGACTAGCAT GGCTGTAGTC TTCCCTCCCA CGGCAACAGC TGTGCCTTCA 
GTATCTGTCC CTGCTACAAG TAGCCCTTTT AATAGTGTTT CTGCACCAGT CATTCAATCT 
GTGGTCACTA TGCCTACTGA AAAGGTAATT GCCGGAGTTG CCTCCAGTGC AGGTGTATTA 
ACTTCAAGTT CTTTTCCTGT ACCACAGGTG TCTGGACCAC CAGTAATGAC CAGTGTGGTT 
TCCAGCATTA CTATTCCTGC AGCCGTGTCA TTGTCTACTG CAAGTGAATC AGTTCAGGCC 
CCACCAGTTG GGGGAGTTAT GTCTAGTGCT GGCACTTTTC CTCCTGCAGC GGGATTGGTA 
GTTCCAGTTC CTGGAATAGC CAGTACAGTT CCCCCAGGTG CTATCACACC AGCTATGACT 
TCTCAGCCCA CCCCAATTGC AACAAATATA ACTGGGGCAG CCACCCTGAC TTCCACATCT 
GCCACCACTC CGTTCCCAAG CATGGCTTCA CAACCACCTC TTCAAATCAG TAGCAGCAGC 
TCTGCTCCCA CTGTAGCAGA AACTGTGGTA GTCAGTGCAC TCCATCCCAA ACACGATGGC 
ACACATTTAC CAGACAAGCC ACCCCTTAGC AGTGCAGCAG GGCTCTCCCT GCCCCTCTCA 
GTAGCTCCAT CTTCCTCTGG AGCTGGGATC TCTGATTCAG CTAGTCAGCC CAGTGTGCTG 
CATCCTTTAC AGGTCCCATC CGCTGTGGCT TCTCCTCCAG TACTTTCACA GGCAGCAGGG 
ACCGTCTCCA CCCCGGTGTT ACCTCAGGTA CCTTTACCTG GTATCCCGCC CCTAGCACAG 
CCTATTACCA ATGTGCCTGC TGTACAGCAG ACACTAATTC ATAGTCAACC TCAACCAGCT 
TCACTTCCCA ACCAGCCCCA CACTCATTGC CCTGAAGTAG ATACTGATAC ACAACCCAAA 
GCCCCTGGCA TTGATGATAT AAAGACTCTG GAAGAAAAAC TGCGGTCTCT CTTCAGTGAA 
CACAGTTCTG TTGGAGCCCA GCATGCCCCT GTCTCCCTGG AGACATCATT GGTAGTGGAG 
ACTACAGTCA TGCCAGGCAT CCCAACCACC GCTGTGGCAC CAAGCAAGCT CATGACTTCC 
ACCACGAGCA CTTGCTTACC ACCAAGCAGT TTGCCTCTAG GAACAACTAC TGGTTTGTCA 
GCTGTTACAC CAGTGGTCAC ACCCGGGCAG GCTGCCACCC CAGTCAGCTA TGTTCCAGCC 
CCCACCAGCA CTGCAGTAGG GGTGAAACCT GGAACCCCTC CCTCAAAGCC ACCTATCGCC 
AGGGTACCGG TACAACCAGT AGGTACTGAA CTTCAATCTG GTACCCCACC CAGCGAGCAG 
CTGCCGCCTT TTCCAGGACC TTCCCTAACA CAGTCTCAGC AACCTCTAGA AGATCTTGAT 
GCTCAGTTAA GAAGAGCCCT TAGTCCAGAG ACTGTCCCAG TCACATCTTC AGTTGCTCCC 
ACATCAGTTG TGACTCCAAC ATCAGTTGCT GGAGCAGTGC CAGTGGTAGT GCCAGCTCAG 
AAAGCAGCTT CAAGCATAGA AGATAATACC ATGATGACCA ACTCAGAGGC TAGCGTTCTT 
AAAATGGGCC GGTTTCAGGT ATCAGTTGCA GTAGATGATT CCTTGAAGGA AAATAGAAGC 
AAGTCGGAAG AAATCAAGTC TGTACACTTT GAATCAAGTA CTTCAGAGTC CTCAGTGCTG 
TCCAGTAGTA GCCCAGAGAA TACACTGGTG AAGCAGGAGC CTGGTGGAGT CACCACAGCT 
CTATGTAGCC CCTCTTTGGA TATGGTTGAC GGTGTTCCTT CTCAAAAAAC CTCTGCTACC 
CAGACAAAGC CCGAAGCTGA GCAGCCTGCC ACAGTGGTTG GCCGATTCTC TGTGTCAAAA 
ACTGAAGATA AGGTCACCCC TGTGGTGGAG GGAGAGCCAC CTGTAGTACG CCCTTTATTG 
GATTCTGAAC AGGCCGTTCC TTCTGCTGTA GCACCAAAGA AAGAAATGCC TGAATCGCCA 
GAGCCAAGCC ATCTTAATGG GCCATCTTGT GACCTAGAAG CTGCCTTTCT CAGTAGGAAT 
ATGGATGATG GTTCAGGTAG CCCCCATTCT ACTCACCATT TGGGCTCAAA AGGCAGCCTT 
CCCATTCAGA GCCTTAGCCA GAGCCTTAGC AATTCATTCA ACTCCTCTTA CATGAGCAGT 
GACAATGAGT CAGACATTGA AGATGAGGAC TTGAAGTCAG AACTACGTCG GCTACGAGAA 
AAGCACCTTA AAGAGATCCA GGACCTACAG AGTCGCCAGA AACATGAAAT CGAGTCTTTA 
TATACCAAAT TGGGCAAAGT CCCTCCTGCA GTCATCATTC CCCCAGCTGC TCCACTCTCA 
GGGAGAAGAA GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGCCGTAG TAGTTCTTTA 
GGAAATAAGA GCCCCCAACT TTCAGGTAAC CTGTCTGCTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACGCTCCA CCCTCCAGGC AGCATCCCAG AGACTGGGCA GAATCAGCTG 
TTACAGCCCC TTAAACCATC TCCCTCCAGT GAGAACCTCT ATTCAGCTTT TACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGCCAAG GGACCAGCAG TACGAATACA 
GTGGGAGGAG CAGTCAATAG CCAAGCCCCA CAAGCCCAGC CGCCCAGTAG AAAGGGCACA 
TTTACAGATG ATCTACACAA GTTGGTAGAC AACTGGGCCC GAGATGCCAT GAATCTCTCA 
GGCAGGAGAG GAAGCAAGGG GCACATGAAC TATGAGGGTC CTGGAATGGC AAGGAAATTT 
TCAGCACCTG GACAGCTCTG CATCTCCATG ACCTCTAATC TGGGTGGTTC TGCCCCCATT 
TCTGCAGCAT CAGCTACCTC TTTAGGTCAC TTCACCAAGG CTATGTGCCC CCCACAGCAG 
TACAGCTTTC CATCTGCCCC TTTCAGCACT CAGTGGAGTG GGACAAGTGG CCCAGCACCG 
CCACCCCTCA GCCAGTTCCA GCCTGTAGGG ACTTCGTCCT TGCAGAGTTT CAACATCAGC 
AATTTGCAAA AATCCATCAG CAATCCTCCA GGTTCAAACC TGAGGACAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_012550819.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_012406273.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa133592
Clone ID Related Accession (Same CDS sequence)	XM_012550819.1
Accession Version	XM_012550819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003843628.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG AGATGTACGA GGAAAAATAT GATGAGTCCG TTGACGTGTA TGCTTTTGGA 
ATGTGCATGC TTGAGATGGC CACATCCGAA TACCCTTATT CTGAGTGCCA AAATGCTGCG 
CAGATCTACC GTCGAGTGAC CAGTGGAGTA AAGCCAGCCA GCTTTGACAA AGTAGCAATT 
CCTGAAGTAA AGGAAATTAT TGAAGGATGC ATACGCCAAA ACAAAGATGA AAGATATGCT 
ATCAAAGACC TTTTGAACCA TGCCTTCTTC CAGGAGGAAA CAGGAGTACG AGTAGAACTA 
GCTGAAGAAG ATGATGGTGA AAAGATAGCC ATTAAGCTAT GGTTACGCAT TGAAGATATT 
AAGAAATTGA AGGGGAAATA CAAAGACAAT GAAGCAATTG AATTTTCCTT TGATTTGGAG 
AGAGATGTCC CAGAAGATGT AGCTCAAGAA ATGGTAGAAT CAGGGTATGT TTGTGAAGGA 
GATCACAAGA CCATGGCCAA AGCCATCAAA GACAGAGTGT CCCTTATAAA GAGAAAACGA 
GAGCAGCGAC AGTTGGTAAG GGAAGAACAG GAGAAAAGAA AGCAAGAAGA GATCAGTTTA 
AAGGAGCAAG TTGAACAGCC AACAACCATT TCTCAGGCTG GGACCAAATT GATTCCTTCT 
ACCCCTGGCA CAGTTACTGT CTCTACCACA TCAGCCTCAG TTTCCACCCA AGTGGAGCCA 
GAAGAACCAG AGGCAGATCA ACACCAACAA CTGCAGTATC AACAACCTAG TCTCTCCGTG 
TTGTCTGATG GAACAGTTGA CAGTGGTCAG GGGTCTTCAG TCTTCACAGA ATCTCGAGTA 
AGCAGCCAGC AGACTGTCTC ATATGGGTCT CAACATGAAC AGGCACTTTC AACAGGCACG 
ATCCCAAGCT ATACAGCTTC TGTTGTTCAA GCACAATCCC AGCCTCATGG TGTATATCCT 
TCCTCCACCG TGACACCGGC ACAGAGCCAG AGTCATCTGG CTTCAAGTAA CTTAACTGGG 
ATTCCATCTT CCCAGACAGC ACAGCTTGCT CAGCAGGGTA TACAACAAGC AGCCCCTCCT 
CAGCAGACAG GCCAGTATCT TCTTCCCCAG TCAGCAGCAT CTGGTGTGCC TACAACTGCA 
CAGCCTGTGA CGCAGCCCCA GCTTCCACAG GTTCTGCCAA TGCCTCAAGT ATCAGTTGGG 
AAGCAGCTTC CTGTTTCTCA GCCAGTATCG ACCATCCAAG GCGAATCTCA GATACCAGTT 
GCAGCACCTT CCCTTCCACA GCCCTCAGTT AACCCATCTC TCTCGGGTGC TCACTTCCTT 
CCTGTGGGCC AGCCACTTCC AGCATCCTTA ATTCCTCAGT TCTCAGTCTC TCAGATTCCC 
ATGTCAACTC CTCATGTCTC TGTGGCTCAG ACAGGTTTCT CATCCCTGCC AAGCTCTATA 
GCATCTGGCA TTAACCAGCC TCTGCTCACC TTGGCCTCAT CTGCTGCAGC GGCTGCTATC 
CCTGTGGGAT CAACTATGGT CCCTAGTCAG CTTCCCACCC TTCTGCAGCC TGTGACTCAG 
CTGCCAAGTC AGGCTCACCC ACAGCTCCTT CAGCCAGCAG TTCAGTCCAT AGGAATACCA 
GCTAACCTTG GACAAGCTGC TGAGGCTTCA CTTCCCACTG GAGATTCTCT ATACCAGGGA 
TTCCCACCTC GACTGCCACC TCAATACCCA GGAGACTCAA ATATTATTCC CTCATCCACC 
GTGGCTTCTG TTTGCGTCCC TTCTACAGTT CTGTCCCCTC CCTTGCCCAC AGAAGCATTG 
GCTCCACCAG GGTACCTTCC TACAGTTGTG CAGCCATATG TCGAGCCAAG TGTTTTAGTT 
CCTGTGGGTA GTCTAGGAGG ACAGGTTCAA GTACCCCATT CAGCAGTCAC CTTAGCACAG 
CAGGCCCCCT CTGCACCCTC CCAGCCAGCA GCTCTGGAGA CACAAGCAGT TTCCCAGTCC 
AGTCCTCCAG AACCTGTGCC ATCAGCACAG TCACAGACCA CTACATTGGT GTCCTCTATA 
GATAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GGAATGAGAA TGTTCCTTCA 
TCCAGTGGGA GACATGAAGG GAGAACTACA AAACGCCATT ACCGAAAGTC TGTACGGAGT 
CGTTCTCGTC ATGAGAAGAC TGCTCGCCCC AAATTGAGAA TTCTTAATGT GTCAAACAAA 
GGAGACAGAG TGGTGGAATG CCAGTTAGAG ACTCACAACC GGAAAATGGT TACATTCAAA 
TTTGACTTGG ATGGTGACAA TCCTGAGGAG ATAGCATCGA TTATGGTGAA CAATGATTTT 
ATTCTAGCAA CAGAGAGAGA CTCCTTTGTG GATCAGGTGC GAGAAATCAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTTAGTGTG GAACCAGAAG GTGATCAAGG ATCAGAAAGC 
TTACAAACAA AGGATGATTA TGGCTTTTCA GGATCCCAGA AATTGGAGGG AGAGTTCAAG 
CAACCAAATC CTGCATCTTC TATGCCTCAG AGGGCAGGTG TTCCCTCCAG TTCATTAACT 
CAAGTTGTAC ATTCAGCTGG ACGGAGGTTC ATAGTGAGTC CTGTACCAGA GAGTCGGTTA 
CGAGAGTCAG TATTTTTCAC TGCTACATCA AGTGAAATAT CAGACACAAC TGCTGTTGCT 
CCATCCCATG GCCCTGTAAT GAATCTGTCT CATTCTGCTT CATCCGTTAG TTTGCAACAG 
GCATTCTCTG AACTTAGACA TGCTCAAATG TCAGAAGGAC CCAGTACAGC TCCCCCAAAC 
TTCAGCCATG CAGGACCAAC CTTTCCAACT GTACCTGCCA CTATGACTAG CATGGCTGTA 
GTCTTCCCTC CCACGGCAAC AGCTGTGCCT TCAGTATCTG TCCCTGCTAC AAGTAGCCCT 
TTTAATAGTG TTTCTGCACC AGTCATTCAA TCTGTGGTCA CTATGCCTAC TGAAAAGGTA 
ATTGCCGGAG TTGCCTCCAG TGCAGGTGTA TTAACTTCAA GTTCTTTTCC TGTACCACAG 
GTGTCTGGAC CACCAGTAAT GACCAGTGTG GTTTCCAGCA TTACTATTCC TGCAGCCGTG 
TCATTGTCTA CTGCAAGTGA ATCAGTTCAG GCCCCACCAG TTGGGGGAGT TATGTCTAGT 
GCTGGCACTT TTCCTCCTGC AGCGGGATTG GTAGTTCCAG TTCCTGGAAT AGCCAGTACA 
GTTCCCCCAG GTGCTATCAC ACCAGCTATG ACTTCTCAGC CCACCCCAAT TGCAACAAAT 
ATAACTGGGG CAGCCACCCT GACTTCCACA TCTGCCACCA CTCCGTTCCC AAGCATGGCT 
TCACAACCAC CTCTTCAAAT CAGTAGCAGC AGCTCTGCTC CCACTGTAGC AGAAACTGTG 
GTAGTCAGTG CACTCCATCC CAAACACGAT GGCACACATT TACCAGACAA GCCACCCCTT 
AGCAGTGCAG CAGGGCTCTC CCTGCCCCTC TCAGTAGCTC CATCTTCCTC TGGAGCTGGG 
ATCTCTGATT CAGCTAGTCA GCCCAGTGTG CTGCATCCTT TACAGGTCCC ATCCGCTGTG 
GCTTCTCCTC CAGTACTTTC ACAGGCAGCA GGGACCGTCT CCACCCCGGT GTTACCTCAG 
GTACCTTTAC CTGGTATCCC GCCCCTAGCA CAGCCTATTA CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA ACCTCAACCA GCTTCACTTC CCAACCAGCC CCACACTCAT 
TGCCCTGAAG TAGATACTGA TACACAACCC AAAGCCCCTG GCATTGATGA TATAAAGACT 
CTGGAAGAAA AACTGCGGTC TCTCTTCAGT GAACACAGTT CTGTTGGAGC CCAGCATGCC 
CCTGTCTCCC TGGAGACATC ATTGGTAGTG GAGACTACAG TCATGCCAGG CATCCCAACC 
ACCGCTGTGG CACCAAGCAA GCTCATGACT TCCACCACGA GCACTTGCTT ACCACCAAGC 
AGTTTGCCTC TAGGAACAAC TACTGGTTTG TCAGCTGTTA CACCAGTGGT CACACCCGGG 
CAGGCTGCCA CCCCAGTCAG CTATGTTCCA GCCCCCACCA GCACTGCAGT AGGGGTGAAA 
CCTGGAACCC CTCCCTCAAA GCCACCTATC GCCAGGGTAC CGGTACAACC AGTAGGTACT 
GAACTTCAAT CTGGTACCCC ACCCAGCGAG CAGCTGCCGC CTTTTCCAGG ACCTTCCCTA 
ACACAGTCTC AGCAACCTCT AGAAGATCTT GATGCTCAGT TAAGAAGAGC CCTTAGTCCA 
GAGACTGTCC CAGTCACATC TTCAGTTGCT CCCACATCAG TTGTGACTCC AACATCAGTT 
GCTGGAGCAG TGCCAGTGGT AGTGCCAGCT CAGAAAGCAG CTTCAAGCAT AGAAGATAAT 
ACCATGATGA CCAACTCAGA GGCTAGCGTT CTTAAAATGG GCCGGTTTCA GGTATCAGTT 
GCAGTAGATG ATTCCTTGAA GGAAAATAGA AGCAAGTCGG AAGAAATCAA GTCTGTACAC 
TTTGAATCAA GTACTTCAGA GTCCTCAGTG CTGTCCAGTA GTAGCCCAGA GAATACACTG 
GTGAAGCAGG AGCCTGGTGG AGTCACCACA GCTCTATGTA GCCCCTCTTT GGATATGGTT 
GACGGTGTTC CTTCTCAAAA AACCTCTGCT ACCCAGACAA AGCCCGAAGC TGAGCAGCCT 
GCCACAGTGG TTGGCCGATT CTCTGTGTCA AAAACTGAAG ATAAGGTCAC CCCTGTGGTG 
GAGGGAGAGC CACCTGTAGT ACGCCCTTTA TTGGATTCTG AACAGGCCGT TCCTTCTGCT 
GTAGCACCAA AGAAAGAAAT GCCTGAATCG CCAGAGCCAA GCCATCTTAA TGGGCCATCT 
TGTGACCTAG AAGCTGCCTT TCTCAGTAGG AATATGGATG ATGGTTCAGG TAGCCCCCAT 
TCTACTCACC ATTTGGGCTC AAAAGGCAGC CTTCCCATTC AGAGCCTTAG CCAGAGCCTT 
AGCAATTCAT TCAACTCCTC TTACATGAGC AGTGACAATG AGTCAGACAT TGAAGATGAG 
GACTTGAAGT CAGAACTACG TCGGCTACGA GAAAAGCACC TTAAAGAGAT CCAGGACCTA 
CAGAGTCGCC AGAAACATGA AATCGAGTCT TTATATACCA AATTGGGCAA AGTCCCTCCT 
GCAGTCATCA TTCCCCCAGC TGCTCCACTC TCAGGGAGAA GAAGACGACC CACTAAAAGC 
AAAGGCAGCA AATCTAGCCG TAGTAGTTCT TTAGGAAATA AGAGCCCCCA ACTTTCAGGT 
AACCTGTCTG CTCAGAGTGC AGCTTCAGTC TTGCACCCCC AGCAGACGCT CCACCCTCCA 
GGCAGCATCC CAGAGACTGG GCAGAATCAG CTGTTACAGC CCCTTAAACC ATCTCCCTCC 
AGTGAGAACC TCTATTCAGC TTTTACCAGT GATGGTGCCA TTTCAGTACC AAGCCTTTCT 
GCTCCAGGCC AAGGGACCAG CAGTACGAAT ACAGTGGGAG GAGCAGTCAA TAGCCAAGCC 
CCACAAGCCC AGCCGCCCAG TAGAAAGGGC ACATTTACAG ATGATCTACA CAAGTTGGTA 
GACAACTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA GGGGCACATG 
AACTATGAGG GTCCTGGAAT GGCAAGGAAA TTTTCAGCAC CTGGACAGCT CTGCATCTCC 
ATGACCTCTA ATCTGGGTGG TTCTGCCCCC ATTTCTGCAG CATCAGCTAC CTCTTTAGGT 
CACTTCACCA AGGCTATGTG CCCCCCACAG CAGTACAGCT TTCCATCTGC CCCTTTCAGC 
ACTCAGTGGA GTGGGACAAG TGGCCCAGCA CCGCCACCCC TCAGCCAGTT CCAGCCTGTA 
GGGACTTCGT CCTTGCAGAG TTTCAACATC AGCAATTTGC AAAAATCCAT CAGCAATCCT 
CCAGGTTCAA ACCTGAGGAC AACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012406273.1
CDS	24..6050
Translation	MAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_012550819.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012550819.1

ORF Insert Sequence:

ATGGCCCCTG AGATGTACGA GGAAAAATAT GATGAGTCCG TTGACGTGTA TGCTTTTGGA 
ATGTGCATGC TTGAGATGGC CACATCCGAA TACCCTTATT CTGAGTGCCA AAATGCTGCG 
CAGATCTACC GTCGAGTGAC CAGTGGAGTA AAGCCAGCCA GCTTTGACAA AGTAGCAATT 
CCTGAAGTAA AGGAAATTAT TGAAGGATGC ATACGCCAAA ACAAAGATGA AAGATATGCT 
ATCAAAGACC TTTTGAACCA TGCCTTCTTC CAGGAGGAAA CAGGAGTACG AGTAGAACTA 
GCTGAAGAAG ATGATGGTGA AAAGATAGCC ATTAAGCTAT GGTTACGCAT TGAAGATATT 
AAGAAATTGA AGGGGAAATA CAAAGACAAT GAAGCAATTG AATTTTCCTT TGATTTGGAG 
AGAGATGTCC CAGAAGATGT AGCTCAAGAA ATGGTAGAAT CAGGGTATGT TTGTGAAGGA 
GATCACAAGA CCATGGCCAA AGCCATCAAA GACAGAGTGT CCCTTATAAA GAGAAAACGA 
GAGCAGCGAC AGTTGGTAAG GGAAGAACAG GAGAAAAGAA AGCAAGAAGA GATCAGTTTA 
AAGGAGCAAG TTGAACAGCC AACAACCATT TCTCAGGCTG GGACCAAATT GATTCCTTCT 
ACCCCTGGCA CAGTTACTGT CTCTACCACA TCAGCCTCAG TTTCCACCCA AGTGGAGCCA 
GAAGAACCAG AGGCAGATCA ACACCAACAA CTGCAGTATC AACAACCTAG TCTCTCCGTG 
TTGTCTGATG GAACAGTTGA CAGTGGTCAG GGGTCTTCAG TCTTCACAGA ATCTCGAGTA 
AGCAGCCAGC AGACTGTCTC ATATGGGTCT CAACATGAAC AGGCACTTTC AACAGGCACG 
ATCCCAAGCT ATACAGCTTC TGTTGTTCAA GCACAATCCC AGCCTCATGG TGTATATCCT 
TCCTCCACCG TGACACCGGC ACAGAGCCAG AGTCATCTGG CTTCAAGTAA CTTAACTGGG 
ATTCCATCTT CCCAGACAGC ACAGCTTGCT CAGCAGGGTA TACAACAAGC AGCCCCTCCT 
CAGCAGACAG GCCAGTATCT TCTTCCCCAG TCAGCAGCAT CTGGTGTGCC TACAACTGCA 
CAGCCTGTGA CGCAGCCCCA GCTTCCACAG GTTCTGCCAA TGCCTCAAGT ATCAGTTGGG 
AAGCAGCTTC CTGTTTCTCA GCCAGTATCG ACCATCCAAG GCGAATCTCA GATACCAGTT 
GCAGCACCTT CCCTTCCACA GCCCTCAGTT AACCCATCTC TCTCGGGTGC TCACTTCCTT 
CCTGTGGGCC AGCCACTTCC AGCATCCTTA ATTCCTCAGT TCTCAGTCTC TCAGATTCCC 
ATGTCAACTC CTCATGTCTC TGTGGCTCAG ACAGGTTTCT CATCCCTGCC AAGCTCTATA 
GCATCTGGCA TTAACCAGCC TCTGCTCACC TTGGCCTCAT CTGCTGCAGC GGCTGCTATC 
CCTGTGGGAT CAACTATGGT CCCTAGTCAG CTTCCCACCC TTCTGCAGCC TGTGACTCAG 
CTGCCAAGTC AGGCTCACCC ACAGCTCCTT CAGCCAGCAG TTCAGTCCAT AGGAATACCA 
GCTAACCTTG GACAAGCTGC TGAGGCTTCA CTTCCCACTG GAGATTCTCT ATACCAGGGA 
TTCCCACCTC GACTGCCACC TCAATACCCA GGAGACTCAA ATATTATTCC CTCATCCACC 
GTGGCTTCTG TTTGCGTCCC TTCTACAGTT CTGTCCCCTC CCTTGCCCAC AGAAGCATTG 
GCTCCACCAG GGTACCTTCC TACAGTTGTG CAGCCATATG TCGAGCCAAG TGTTTTAGTT 
CCTGTGGGTA GTCTAGGAGG ACAGGTTCAA GTACCCCATT CAGCAGTCAC CTTAGCACAG 
CAGGCCCCCT CTGCACCCTC CCAGCCAGCA GCTCTGGAGA CACAAGCAGT TTCCCAGTCC 
AGTCCTCCAG AACCTGTGCC ATCAGCACAG TCACAGACCA CTACATTGGT GTCCTCTATA 
GATAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GGAATGAGAA TGTTCCTTCA 
TCCAGTGGGA GACATGAAGG GAGAACTACA AAACGCCATT ACCGAAAGTC TGTACGGAGT 
CGTTCTCGTC ATGAGAAGAC TGCTCGCCCC AAATTGAGAA TTCTTAATGT GTCAAACAAA 
GGAGACAGAG TGGTGGAATG CCAGTTAGAG ACTCACAACC GGAAAATGGT TACATTCAAA 
TTTGACTTGG ATGGTGACAA TCCTGAGGAG ATAGCATCGA TTATGGTGAA CAATGATTTT 
ATTCTAGCAA CAGAGAGAGA CTCCTTTGTG GATCAGGTGC GAGAAATCAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTTAGTGTG GAACCAGAAG GTGATCAAGG ATCAGAAAGC 
TTACAAACAA AGGATGATTA TGGCTTTTCA GGATCCCAGA AATTGGAGGG AGAGTTCAAG 
CAACCAAATC CTGCATCTTC TATGCCTCAG AGGGCAGGTG TTCCCTCCAG TTCATTAACT 
CAAGTTGTAC ATTCAGCTGG ACGGAGGTTC ATAGTGAGTC CTGTACCAGA GAGTCGGTTA 
CGAGAGTCAG TATTTTTCAC TGCTACATCA AGTGAAATAT CAGACACAAC TGCTGTTGCT 
CCATCCCATG GCCCTGTAAT GAATCTGTCT CATTCTGCTT CATCCGTTAG TTTGCAACAG 
GCATTCTCTG AACTTAGACA TGCTCAAATG TCAGAAGGAC CCAGTACAGC TCCCCCAAAC 
TTCAGCCATG CAGGACCAAC CTTTCCAACT GTACCTGCCA CTATGACTAG CATGGCTGTA 
GTCTTCCCTC CCACGGCAAC AGCTGTGCCT TCAGTATCTG TCCCTGCTAC AAGTAGCCCT 
TTTAATAGTG TTTCTGCACC AGTCATTCAA TCTGTGGTCA CTATGCCTAC TGAAAAGGTA 
ATTGCCGGAG TTGCCTCCAG TGCAGGTGTA TTAACTTCAA GTTCTTTTCC TGTACCACAG 
GTGTCTGGAC CACCAGTAAT GACCAGTGTG GTTTCCAGCA TTACTATTCC TGCAGCCGTG 
TCATTGTCTA CTGCAAGTGA ATCAGTTCAG GCCCCACCAG TTGGGGGAGT TATGTCTAGT 
GCTGGCACTT TTCCTCCTGC AGCGGGATTG GTAGTTCCAG TTCCTGGAAT AGCCAGTACA 
GTTCCCCCAG GTGCTATCAC ACCAGCTATG ACTTCTCAGC CCACCCCAAT TGCAACAAAT 
ATAACTGGGG CAGCCACCCT GACTTCCACA TCTGCCACCA CTCCGTTCCC AAGCATGGCT 
TCACAACCAC CTCTTCAAAT CAGTAGCAGC AGCTCTGCTC CCACTGTAGC AGAAACTGTG 
GTAGTCAGTG CACTCCATCC CAAACACGAT GGCACACATT TACCAGACAA GCCACCCCTT 
AGCAGTGCAG CAGGGCTCTC CCTGCCCCTC TCAGTAGCTC CATCTTCCTC TGGAGCTGGG 
ATCTCTGATT CAGCTAGTCA GCCCAGTGTG CTGCATCCTT TACAGGTCCC ATCCGCTGTG 
GCTTCTCCTC CAGTACTTTC ACAGGCAGCA GGGACCGTCT CCACCCCGGT GTTACCTCAG 
GTACCTTTAC CTGGTATCCC GCCCCTAGCA CAGCCTATTA CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA ACCTCAACCA GCTTCACTTC CCAACCAGCC CCACACTCAT 
TGCCCTGAAG TAGATACTGA TACACAACCC AAAGCCCCTG GCATTGATGA TATAAAGACT 
CTGGAAGAAA AACTGCGGTC TCTCTTCAGT GAACACAGTT CTGTTGGAGC CCAGCATGCC 
CCTGTCTCCC TGGAGACATC ATTGGTAGTG GAGACTACAG TCATGCCAGG CATCCCAACC 
ACCGCTGTGG CACCAAGCAA GCTCATGACT TCCACCACGA GCACTTGCTT ACCACCAAGC 
AGTTTGCCTC TAGGAACAAC TACTGGTTTG TCAGCTGTTA CACCAGTGGT CACACCCGGG 
CAGGCTGCCA CCCCAGTCAG CTATGTTCCA GCCCCCACCA GCACTGCAGT AGGGGTGAAA 
CCTGGAACCC CTCCCTCAAA GCCACCTATC GCCAGGGTAC CGGTACAACC AGTAGGTACT 
GAACTTCAAT CTGGTACCCC ACCCAGCGAG CAGCTGCCGC CTTTTCCAGG ACCTTCCCTA 
ACACAGTCTC AGCAACCTCT AGAAGATCTT GATGCTCAGT TAAGAAGAGC CCTTAGTCCA 
GAGACTGTCC CAGTCACATC TTCAGTTGCT CCCACATCAG TTGTGACTCC AACATCAGTT 
GCTGGAGCAG TGCCAGTGGT AGTGCCAGCT CAGAAAGCAG CTTCAAGCAT AGAAGATAAT 
ACCATGATGA CCAACTCAGA GGCTAGCGTT CTTAAAATGG GCCGGTTTCA GGTATCAGTT 
GCAGTAGATG ATTCCTTGAA GGAAAATAGA AGCAAGTCGG AAGAAATCAA GTCTGTACAC 
TTTGAATCAA GTACTTCAGA GTCCTCAGTG CTGTCCAGTA GTAGCCCAGA GAATACACTG 
GTGAAGCAGG AGCCTGGTGG AGTCACCACA GCTCTATGTA GCCCCTCTTT GGATATGGTT 
GACGGTGTTC CTTCTCAAAA AACCTCTGCT ACCCAGACAA AGCCCGAAGC TGAGCAGCCT 
GCCACAGTGG TTGGCCGATT CTCTGTGTCA AAAACTGAAG ATAAGGTCAC CCCTGTGGTG 
GAGGGAGAGC CACCTGTAGT ACGCCCTTTA TTGGATTCTG AACAGGCCGT TCCTTCTGCT 
GTAGCACCAA AGAAAGAAAT GCCTGAATCG CCAGAGCCAA GCCATCTTAA TGGGCCATCT 
TGTGACCTAG AAGCTGCCTT TCTCAGTAGG AATATGGATG ATGGTTCAGG TAGCCCCCAT 
TCTACTCACC ATTTGGGCTC AAAAGGCAGC CTTCCCATTC AGAGCCTTAG CCAGAGCCTT 
AGCAATTCAT TCAACTCCTC TTACATGAGC AGTGACAATG AGTCAGACAT TGAAGATGAG 
GACTTGAAGT CAGAACTACG TCGGCTACGA GAAAAGCACC TTAAAGAGAT CCAGGACCTA 
CAGAGTCGCC AGAAACATGA AATCGAGTCT TTATATACCA AATTGGGCAA AGTCCCTCCT 
GCAGTCATCA TTCCCCCAGC TGCTCCACTC TCAGGGAGAA GAAGACGACC CACTAAAAGC 
AAAGGCAGCA AATCTAGCCG TAGTAGTTCT TTAGGAAATA AGAGCCCCCA ACTTTCAGGT 
AACCTGTCTG CTCAGAGTGC AGCTTCAGTC TTGCACCCCC AGCAGACGCT CCACCCTCCA 
GGCAGCATCC CAGAGACTGG GCAGAATCAG CTGTTACAGC CCCTTAAACC ATCTCCCTCC 
AGTGAGAACC TCTATTCAGC TTTTACCAGT GATGGTGCCA TTTCAGTACC AAGCCTTTCT 
GCTCCAGGCC AAGGGACCAG CAGTACGAAT ACAGTGGGAG GAGCAGTCAA TAGCCAAGCC 
CCACAAGCCC AGCCGCCCAG TAGAAAGGGC ACATTTACAG ATGATCTACA CAAGTTGGTA 
GACAACTGGG CCCGAGATGC CATGAATCTC TCAGGCAGGA GAGGAAGCAA GGGGCACATG 
AACTATGAGG GTCCTGGAAT GGCAAGGAAA TTTTCAGCAC CTGGACAGCT CTGCATCTCC 
ATGACCTCTA ATCTGGGTGG TTCTGCCCCC ATTTCTGCAG CATCAGCTAC CTCTTTAGGT 
CACTTCACCA AGGCTATGTG CCCCCCACAG CAGTACAGCT TTCCATCTGC CCCTTTCAGC 
ACTCAGTGGA GTGGGACAAG TGGCCCAGCA CCGCCACCCC TCAGCCAGTT CCAGCCTGTA 
GGGACTTCGT CCTTGCAGAG TTTCAACATC AGCAATTTGC AAAAATCCAT CAGCAATCCT 
CCAGGTTCAA ACCTGAGGAC AACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939531.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795391.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

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CloneID	OSa237672
Clone ID Related Accession (Same CDS sequence)	XM_031939531.1
Accession Version	XM_031939531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGCTGTG CGAAGTTTAA CTGTGCATCT GAGCGGGTGA CATTCAAACC TGGTGGCAGG 
AGGACCCGAT TTCTGAGTAC GCCCTGCTTG GCTCTTTGGA AGATGGTGAA AAAAGTCTGT 
CCTTGCAACC AGCTCTGTAG GACCAGCAGT ACGAATACAG TGGGAGGAGC AGTCAATAGC 
CAAGCCCCAC AAGCCCAGCC GCCCAGTAGA AAGGGCACAT TTACAGATGA TCTACACAAG 
TTGGTAGACA ACTGGGCCCG AGATGCCATG AATCTCTCAG GCAGGAGAGG AAGCAAGGGG 
CACATGAACT ATGAGGGTCC TGGAATGGCA AGGAAATTTT CAGCACCTGG ACAGCTCTGC 
ATCTCCATGA CCTCTAATCT GGGTGGTTCT GCCCCCATTT CTGCAGCATC AGCTACCTCT 
TTAGGTCACT TCACCAAGGC TATGTGCCCC CCACAGCAGT ACAGCTTTCC ATCTGCCCCT 
TTCAGCACTC AGTGGAGTGG GACAAGTGGC CCAGCACCGC CACCCCTCAG CCAGTTCCAG 
CCTGTAGGGA CTTCGTCCTT GCAGAGTTTC AACATCAGCA ATTTGCAAAA ATCCATCAGC 
AATCCTCCAG GTTCAAACCT GAGGACAACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795391.1
CDS	1..7353
Translation	MSGGSVEKQSGPAGPRFLSPPAVPPKNSSSSDTSVGEKLGAAGAEPGAGRTEEYRRRRHTMDKDSRGSAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPGPVAAATAAPPPTLAPEPHREEPLTAPAASPAAPQPPAPPAPADHPNGGPASAAGRDPAVKHAPTPAASNHVGSKEEPAAAAAAAAAASGSAGAREQEERSQQQDDNEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGCAKFNCASERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_031939531.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939531.1

ORF Insert Sequence:

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGCTGTG CGAAGTTTAA CTGTGCATCT GAGCGGGTGA CATTCAAACC TGGTGGCAGG 
AGGACCCGAT TTCTGAGTAC GCCCTGCTTG GCTCTTTGGA AGATGGTGAA AAAAGTCTGT 
CCTTGCAACC AGCTCTGTAG GACCAGCAGT ACGAATACAG TGGGAGGAGC AGTCAATAGC 
CAAGCCCCAC AAGCCCAGCC GCCCAGTAGA AAGGGCACAT TTACAGATGA TCTACACAAG 
TTGGTAGACA ACTGGGCCCG AGATGCCATG AATCTCTCAG GCAGGAGAGG AAGCAAGGGG 
CACATGAACT ATGAGGGTCC TGGAATGGCA AGGAAATTTT CAGCACCTGG ACAGCTCTGC 
ATCTCCATGA CCTCTAATCT GGGTGGTTCT GCCCCCATTT CTGCAGCATC AGCTACCTCT 
TTAGGTCACT TCACCAAGGC TATGTGCCCC CCACAGCAGT ACAGCTTTCC ATCTGCCCCT 
TTCAGCACTC AGTGGAGTGG GACAAGTGGC CCAGCACCGC CACCCCTCAG CCAGTTCCAG 
CCTGTAGGGA CTTCGTCCTT GCAGAGTTTC AACATCAGCA ATTTGCAAAA ATCCATCAGC 
AATCCTCCAG GTTCAAACCT GAGGACAACT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939533.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795393.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

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CloneID	OSa237673
Clone ID Related Accession (Same CDS sequence)	XM_031939533.1
Accession Version	XM_031939533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCT TCAAGCATAG AAGATAATAC CATGATGACC 
AACTCAGAGG CTAGCGTTCT TAAAATGGGC CGGTTTCAGG TATCAGTTGC AGTAGATGAT 
TCCTTGAAGG AAAATAGAAG CAAGTCGGAA GAAATCAAGT CTGTACACTT TGAATCAAGT 
ACTTCAGAGT CCTCAGTGCT GTCCAGTAGT AGCCCAGAGA ATACACTGGT GAAGCAGGAG 
CCTGGTGGAG TCACCACAGC TCTATGTAGC CCCTCTTTGG ATATGGTTGA CGGTGTTCCT 
TCTCAAAAAA CCTCTGCTAC CCAGACAAAG CCCGAAGCTG AGCAGCCTGC CACAGTGGTT 
GGCCGATTCT CTGTGTCAAA AACTGAAGAT AAGGTCACCC CTGTGGTGGA GGGAGAGCCA 
CCTGTAGTAC GCCCTTTATT GGATTCTGAA CAGGCCGTTC CTTCTGCTGT AGCACCAAAG 
AAAGAAATGC CTGAATCGCC AGAGCCAAGC CATCTTAATG GGCCATCTTG TGACCTAGAA 
GCTGCCTTTC TCAGTAGGAA TATGGATGAT GGTTCAGGTA GCCCCCATTC TACTCACCAT 
TTGGGCTCAA AAGGCAGCCT TCCCATTCAG AGCCTTAGCC AGAGCCTTAG CAATTCATTC 
AACTCCTCTT ACATGAGCAG TGACAATGAG TCAGACATTG AAGATGAGGA CTTGAAGTCA 
GAACTACGTC GGCTACGAGA AAAGCACCTT AAAGAGATCC AGGACCTACA GAGTCGCCAG 
AAACATGAAA TCGAGTCTTT ATATACCAAA TTGGGCAAAG TCCCTCCTGC AGTCATCATT 
CCCCCAGCTG CTCCACTCTC AGGGAGAAGA AGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGCCGTA GTAGTTCTTT AGGAAATAAG AGCCCCCAAC TTTCAGGTAA CCTGTCTGCT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACGCTCC ACCCTCCAGG CAGCATCCCA 
GAGACTGGGC AGAATCAGCT GTTACAGCCC CTTAAACCAT CTCCCTCCAG TGAGAACCTC 
TATTCAGCTT TTACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGCCAA 
GGCTGTGCGA AGTTTAACTG TGCATCTGAG CGGGTGACAT TCAAACCTGG TGGCAGGAGG 
ACCCGATTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT 
TGCAACCAGC TCTGTAGGAC CAGCAGTACG AATACAGTGG GAGGAGCAGT CAATAGCCAA 
GCCCCACAAG CCCAGCCGCC CAGTAGAAAG GGCACATTTA CAGATGATCT ACACAAGTTG 
GTAGACAACT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAAGGGGCAC 
ATGAACTATG AGGGTCCTGG AATGGCAAGG AAATTTTCAG CACCTGGACA GCTCTGCATC 
TCCATGACCT CTAATCTGGG TGGTTCTGCC CCCATTTCTG CAGCATCAGC TACCTCTTTA 
GGTCACTTCA CCAAGGCTAT GTGCCCCCCA CAGCAGTACA GCTTTCCATC TGCCCCTTTC 
AGCACTCAGT GGAGTGGGAC AAGTGGCCCA GCACCGCCAC CCCTCAGCCA GTTCCAGCCT 
GTAGGGACTT CGTCCTTGCA GAGTTTCAAC ATCAGCAATT TGCAAAAATC CATCAGCAAT 
CCTCCAGGTT CAAACCTGAG GACAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795393.1
CDS	1085..8434
Translation	MSGGSVEKQSGPAGPRFLSPPAVPPKNSSSSDTSVGEKLGAAGAEPGAGRTEEYRRRRHTMDKDSRGSAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPGPVAAATAAPPPTLAPEPHREEPLTAPAASPAAPQPPAPPAPADHPNGGPASAAGRDPAVKHAPTPAASNHVGSKEEPAAAAAAAAAASGSAGAREQEERSQQQDDNEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGCAKFNCASERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_031939533.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939533.1

ORF Insert Sequence:

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCT TCAAGCATAG AAGATAATAC CATGATGACC 
AACTCAGAGG CTAGCGTTCT TAAAATGGGC CGGTTTCAGG TATCAGTTGC AGTAGATGAT 
TCCTTGAAGG AAAATAGAAG CAAGTCGGAA GAAATCAAGT CTGTACACTT TGAATCAAGT 
ACTTCAGAGT CCTCAGTGCT GTCCAGTAGT AGCCCAGAGA ATACACTGGT GAAGCAGGAG 
CCTGGTGGAG TCACCACAGC TCTATGTAGC CCCTCTTTGG ATATGGTTGA CGGTGTTCCT 
TCTCAAAAAA CCTCTGCTAC CCAGACAAAG CCCGAAGCTG AGCAGCCTGC CACAGTGGTT 
GGCCGATTCT CTGTGTCAAA AACTGAAGAT AAGGTCACCC CTGTGGTGGA GGGAGAGCCA 
CCTGTAGTAC GCCCTTTATT GGATTCTGAA CAGGCCGTTC CTTCTGCTGT AGCACCAAAG 
AAAGAAATGC CTGAATCGCC AGAGCCAAGC CATCTTAATG GGCCATCTTG TGACCTAGAA 
GCTGCCTTTC TCAGTAGGAA TATGGATGAT GGTTCAGGTA GCCCCCATTC TACTCACCAT 
TTGGGCTCAA AAGGCAGCCT TCCCATTCAG AGCCTTAGCC AGAGCCTTAG CAATTCATTC 
AACTCCTCTT ACATGAGCAG TGACAATGAG TCAGACATTG AAGATGAGGA CTTGAAGTCA 
GAACTACGTC GGCTACGAGA AAAGCACCTT AAAGAGATCC AGGACCTACA GAGTCGCCAG 
AAACATGAAA TCGAGTCTTT ATATACCAAA TTGGGCAAAG TCCCTCCTGC AGTCATCATT 
CCCCCAGCTG CTCCACTCTC AGGGAGAAGA AGACGACCCA CTAAAAGCAA AGGCAGCAAA 
TCTAGCCGTA GTAGTTCTTT AGGAAATAAG AGCCCCCAAC TTTCAGGTAA CCTGTCTGCT 
CAGAGTGCAG CTTCAGTCTT GCACCCCCAG CAGACGCTCC ACCCTCCAGG CAGCATCCCA 
GAGACTGGGC AGAATCAGCT GTTACAGCCC CTTAAACCAT CTCCCTCCAG TGAGAACCTC 
TATTCAGCTT TTACCAGTGA TGGTGCCATT TCAGTACCAA GCCTTTCTGC TCCAGGCCAA 
GGCTGTGCGA AGTTTAACTG TGCATCTGAG CGGGTGACAT TCAAACCTGG TGGCAGGAGG 
ACCCGATTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT 
TGCAACCAGC TCTGTAGGAC CAGCAGTACG AATACAGTGG GAGGAGCAGT CAATAGCCAA 
GCCCCACAAG CCCAGCCGCC CAGTAGAAAG GGCACATTTA CAGATGATCT ACACAAGTTG 
GTAGACAACT GGGCCCGAGA TGCCATGAAT CTCTCAGGCA GGAGAGGAAG CAAGGGGCAC 
ATGAACTATG AGGGTCCTGG AATGGCAAGG AAATTTTCAG CACCTGGACA GCTCTGCATC 
TCCATGACCT CTAATCTGGG TGGTTCTGCC CCCATTTCTG CAGCATCAGC TACCTCTTTA 
GGTCACTTCA CCAAGGCTAT GTGCCCCCCA CAGCAGTACA GCTTTCCATC TGCCCCTTTC 
AGCACTCAGT GGAGTGGGAC AAGTGGCCCA GCACCGCCAC CCCTCAGCCA GTTCCAGCCT 
GTAGGGACTT CGTCCTTGCA GAGTTTCAAC ATCAGCAATT TGCAAAAATC CATCAGCAAT 
CCTCCAGGTT CAAACCTGAG GACAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939534.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795394.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

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CloneID	OSa237674
Clone ID Related Accession (Same CDS sequence)	XM_031939534.1
Accession Version	XM_031939534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGCTGTG CGAAGTTTAA CTGTGCATCT GAGCGGGTGA CATTCAAACC TGGTGGCAGG 
AGGACCCGAT TTCTGAGGAA GATGGTGAAA AAAGTCTGTC CTTGCAACCA GCTCTGTAGG 
ACCAGCAGTA CGAATACAGT GGGAGGAGCA GTCAATAGCC AAGCCCCACA AGCCCAGCCG 
CCCAGTAGAA AGGGCACATT TACAGATGAT CTACACAAGT TGGTAGACAA CTGGGCCCGA 
GATGCCATGA ATCTCTCAGG CAGGAGAGGA AGCAAGGGGC ACATGAACTA TGAGGGTCCT 
GGAATGGCAA GGAAATTTTC AGCACCTGGA CAGCTCTGCA TCTCCATGAC CTCTAATCTG 
GGTGGTTCTG CCCCCATTTC TGCAGCATCA GCTACCTCTT TAGGTCACTT CACCAAGGCT 
ATGTGCCCCC CACAGCAGTA CAGCTTTCCA TCTGCCCCTT TCAGCACTCA GTGGAGTGGG 
ACAAGTGGCC CAGCACCGCC ACCCCTCAGC CAGTTCCAGC CTGTAGGGAC TTCGTCCTTG 
CAGAGTTTCA ACATCAGCAA TTTGCAAAAA TCCATCAGCA ATCCTCCAGG TTCAAACCTG 
AGGACAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795394.1
CDS	1085..8416
Translation	MSGGSVEKQSGPAGPRFLSPPAVPPKNSSSSDTSVGEKLGAAGAEPGAGRTEEYRRRRHTMDKDSRGSAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPGPVAAATAAPPPTLAPEPHREEPLTAPAASPAAPQPPAPPAPADHPNGGPASAAGRDPAVKHAPTPAASNHVGSKEEPAAAAAAAAAASGSAGAREQEERSQQQDDNEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGCAKFNCASERVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_031939534.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939534.1

ORF Insert Sequence:

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGCTGTG CGAAGTTTAA CTGTGCATCT GAGCGGGTGA CATTCAAACC TGGTGGCAGG 
AGGACCCGAT TTCTGAGGAA GATGGTGAAA AAAGTCTGTC CTTGCAACCA GCTCTGTAGG 
ACCAGCAGTA CGAATACAGT GGGAGGAGCA GTCAATAGCC AAGCCCCACA AGCCCAGCCG 
CCCAGTAGAA AGGGCACATT TACAGATGAT CTACACAAGT TGGTAGACAA CTGGGCCCGA 
GATGCCATGA ATCTCTCAGG CAGGAGAGGA AGCAAGGGGC ACATGAACTA TGAGGGTCCT 
GGAATGGCAA GGAAATTTTC AGCACCTGGA CAGCTCTGCA TCTCCATGAC CTCTAATCTG 
GGTGGTTCTG CCCCCATTTC TGCAGCATCA GCTACCTCTT TAGGTCACTT CACCAAGGCT 
ATGTGCCCCC CACAGCAGTA CAGCTTTCCA TCTGCCCCTT TCAGCACTCA GTGGAGTGGG 
ACAAGTGGCC CAGCACCGCC ACCCCTCAGC CAGTTCCAGC CTGTAGGGAC TTCGTCCTTG 
CAGAGTTTCA ACATCAGCAA TTTGCAAAAA TCCATCAGCA ATCCTCCAGG TTCAAACCTG 
AGGACAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939535.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795395.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

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CloneID	OSa237675
Clone ID Related Accession (Same CDS sequence)	XM_031939535.1
Accession Version	XM_031939535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGCCC 
ACATCAGTTG TGACTCCAAC ATCAGTTGCT GGAGCAGTGC CAGTGGTAGT GCCAGCTCAG 
AAAGCAGCTT CAAGCATAGA AGATAATACC ATGATGACCA ACTCAGAGGC TAGCGTTCTT 
AAAATGGGCC GGTTTCAGGT ATCAGTTGCA GTAGATGATT CCTTGAAGGA AAATAGAAGC 
AAGTCGGAAG AAATCAAGTC TGTACACTTT GAATCAAGTA CTTCAGAGTC CTCAGTGCTG 
TCCAGTAGTA GCCCAGAGAA TACACTGGTG AAGCAGGAGC CTGGTGGAGT CACCACAGCT 
CTATGTAGCC CCTCTTTGGA TATGGTTGAC GGTGTTCCTT CTCAAAAAAC CTCTGCTACC 
CAGACAAAGC CCGAAGCTGA GCAGCCTGCC ACAGTGGTTG GCCGATTCTC TGTGTCAAAA 
ACTGAAGATA AGGTCACCCC TGTGGTGGAG GGAGAGCCAC CTGTAGTACG CCCTTTATTG 
GATTCTGAAC AGGCCGTTCC TTCTGCTGTA GCACCAAAGA AAGAAATGCC TGAATCGCCA 
GAGCCAAGCC ATCTTAATGG GCCATCTTGT GACCTAGAAG CTGCCTTTCT CAGTAGGAAT 
ATGGATGATG GTTCAGGTAG CCCCCATTCT ACTCACCATT TGGGCTCAAA AGGCAGCCTT 
CCCATTCAGA GCCTTAGCCA GAGCCTTAGC AATTCATTCA ACTCCTCTTA CATGAGCAGT 
GACAATGAGT CAGACATTGA AGATGAGGAC TTGAAGTCAG AACTACGTCG GCTACGAGAA 
AAGCACCTTA AAGAGATCCA GGACCTACAG AGTCGCCAGA AACATGAAAT CGAGTCTTTA 
TATACCAAAT TGGGCAAAGT CCCTCCTGCA GTCATCATTC CCCCAGCTGC TCCACTCTCA 
GGGAGAAGAA GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGCCGTAG TAGTTCTTTA 
GGAAATAAGA GCCCCCAACT TTCAGGTAAC CTGTCTGCTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACGCTCCA CCCTCCAGGC AGCATCCCAG AGACTGGGCA GAATCAGCTG 
TTACAGCCCC TTAAACCATC TCCCTCCAGT GAGAACCTCT ATTCAGCTTT TACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGCCAAG GCTGTGCGAA GTTTAACTGT 
GCATCTGAGC GGGTGACATT CAAACCTGGT GGCAGGAGGA CCCGATTTCT GAGTACGCCC 
TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA GTCTGTCCTT GCAACCAGCT CTGTAGGACC 
AGCAGTACGA ATACAGTGGG AGGAGCAGTC AATAGCCAAG CCCCACAAGC CCAGCCGCCC 
AGTAGAAAGG GCACATTTAC AGATGATCTA CACAAGTTGG TAGACAACTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAGGGGCACA TGAACTATGA GGGTCCTGGA 
ATGGCAAGGA AATTTTCAGC ACCTGGACAG CTCTGCATCT CCATGACCTC TAATCTGGGT 
GGTTCTGCCC CCATTTCTGC AGCATCAGCT ACCTCTTTAG GTCACTTCAC CAAGGCTATG 
TGCCCCCCAC AGCAGTACAG CTTTCCATCT GCCCCTTTCA GCACTCAGTG GAGTGGGACA 
AGTGGCCCAG CACCGCCACC CCTCAGCCAG TTCCAGCCTG TAGGGACTTC GTCCTTGCAG 
AGTTTCAACA TCAGCAATTT GCAAAAATCC ATCAGCAATC CTCCAGGTTC AAACCTGAGG 
ACAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795395.1
CDS	1085..8353
Translation	MSGGSVEKQSGPAGPRFLSPPAVPPKNSSSSDTSVGEKLGAAGAEPGAGRTEEYRRRRHTMDKDSRGSAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPGPVAAATAAPPPTLAPEPHREEPLTAPAASPAAPQPPAPPAPADHPNGGPASAAGRDPAVKHAPTPAASNHVGSKEEPAAAAAAAAAASGSAGAREQEERSQQQDDNEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGCAKFNCASERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_031939535.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939535.1

ORF Insert Sequence:

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGCCC 
ACATCAGTTG TGACTCCAAC ATCAGTTGCT GGAGCAGTGC CAGTGGTAGT GCCAGCTCAG 
AAAGCAGCTT CAAGCATAGA AGATAATACC ATGATGACCA ACTCAGAGGC TAGCGTTCTT 
AAAATGGGCC GGTTTCAGGT ATCAGTTGCA GTAGATGATT CCTTGAAGGA AAATAGAAGC 
AAGTCGGAAG AAATCAAGTC TGTACACTTT GAATCAAGTA CTTCAGAGTC CTCAGTGCTG 
TCCAGTAGTA GCCCAGAGAA TACACTGGTG AAGCAGGAGC CTGGTGGAGT CACCACAGCT 
CTATGTAGCC CCTCTTTGGA TATGGTTGAC GGTGTTCCTT CTCAAAAAAC CTCTGCTACC 
CAGACAAAGC CCGAAGCTGA GCAGCCTGCC ACAGTGGTTG GCCGATTCTC TGTGTCAAAA 
ACTGAAGATA AGGTCACCCC TGTGGTGGAG GGAGAGCCAC CTGTAGTACG CCCTTTATTG 
GATTCTGAAC AGGCCGTTCC TTCTGCTGTA GCACCAAAGA AAGAAATGCC TGAATCGCCA 
GAGCCAAGCC ATCTTAATGG GCCATCTTGT GACCTAGAAG CTGCCTTTCT CAGTAGGAAT 
ATGGATGATG GTTCAGGTAG CCCCCATTCT ACTCACCATT TGGGCTCAAA AGGCAGCCTT 
CCCATTCAGA GCCTTAGCCA GAGCCTTAGC AATTCATTCA ACTCCTCTTA CATGAGCAGT 
GACAATGAGT CAGACATTGA AGATGAGGAC TTGAAGTCAG AACTACGTCG GCTACGAGAA 
AAGCACCTTA AAGAGATCCA GGACCTACAG AGTCGCCAGA AACATGAAAT CGAGTCTTTA 
TATACCAAAT TGGGCAAAGT CCCTCCTGCA GTCATCATTC CCCCAGCTGC TCCACTCTCA 
GGGAGAAGAA GACGACCCAC TAAAAGCAAA GGCAGCAAAT CTAGCCGTAG TAGTTCTTTA 
GGAAATAAGA GCCCCCAACT TTCAGGTAAC CTGTCTGCTC AGAGTGCAGC TTCAGTCTTG 
CACCCCCAGC AGACGCTCCA CCCTCCAGGC AGCATCCCAG AGACTGGGCA GAATCAGCTG 
TTACAGCCCC TTAAACCATC TCCCTCCAGT GAGAACCTCT ATTCAGCTTT TACCAGTGAT 
GGTGCCATTT CAGTACCAAG CCTTTCTGCT CCAGGCCAAG GCTGTGCGAA GTTTAACTGT 
GCATCTGAGC GGGTGACATT CAAACCTGGT GGCAGGAGGA CCCGATTTCT GAGTACGCCC 
TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA GTCTGTCCTT GCAACCAGCT CTGTAGGACC 
AGCAGTACGA ATACAGTGGG AGGAGCAGTC AATAGCCAAG CCCCACAAGC CCAGCCGCCC 
AGTAGAAAGG GCACATTTAC AGATGATCTA CACAAGTTGG TAGACAACTG GGCCCGAGAT 
GCCATGAATC TCTCAGGCAG GAGAGGAAGC AAGGGGCACA TGAACTATGA GGGTCCTGGA 
ATGGCAAGGA AATTTTCAGC ACCTGGACAG CTCTGCATCT CCATGACCTC TAATCTGGGT 
GGTTCTGCCC CCATTTCTGC AGCATCAGCT ACCTCTTTAG GTCACTTCAC CAAGGCTATG 
TGCCCCCCAC AGCAGTACAG CTTTCCATCT GCCCCTTTCA GCACTCAGTG GAGTGGGACA 
AGTGGCCCAG CACCGCCACC CCTCAGCCAG TTCCAGCCTG TAGGGACTTC GTCCTTGCAG 
AGTTTCAACA TCAGCAATTT GCAAAAATCC ATCAGCAATC CTCCAGGTTC AAACCTGAGG 
ACAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939536.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795396.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

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CloneID	OSa237676
Clone ID Related Accession (Same CDS sequence)	XM_031939536.1
Accession Version	XM_031939536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGCCC 
ACATCAGTTG TGACTCCAAC ATCAGTTGCT GGAGCAGTGC CAGTGGTAGT GCCAGCTCAG 
AAAGCTTCAA GCATAGAAGA TAATACCATG ATGACCAACT CAGAGGCTAG CGTTCTTAAA 
ATGGGCCGGT TTCAGGTATC AGTTGCAGTA GATGATTCCT TGAAGGAAAA TAGAAGCAAG 
TCGGAAGAAA TCAAGTCTGT ACACTTTGAA TCAAGTACTT CAGAGTCCTC AGTGCTGTCC 
AGTAGTAGCC CAGAGAATAC ACTGGTGAAG CAGGAGCCTG GTGGAGTCAC CACAGCTCTA 
TGTAGCCCCT CTTTGGATAT GGTTGACGGT GTTCCTTCTC AAAAAACCTC TGCTACCCAG 
ACAAAGCCCG AAGCTGAGCA GCCTGCCACA GTGGTTGGCC GATTCTCTGT GTCAAAAACT 
GAAGATAAGG TCACCCCTGT GGTGGAGGGA GAGCCACCTG TAGTACGCCC TTTATTGGAT 
TCTGAACAGG CCGTTCCTTC TGCTGTAGCA CCAAAGAAAG AAATGCCTGA ATCGCCAGAG 
CCAAGCCATC TTAATGGGCC ATCTTGTGAC CTAGAAGCTG CCTTTCTCAG TAGGAATATG 
GATGATGGTT CAGGTAGCCC CCATTCTACT CACCATTTGG GCTCAAAAGG CAGCCTTCCC 
ATTCAGAGCC TTAGCCAGAG CCTTAGCAAT TCATTCAACT CCTCTTACAT GAGCAGTGAC 
AATGAGTCAG ACATTGAAGA TGAGGACTTG AAGTCAGAAC TACGTCGGCT ACGAGAAAAG 
CACCTTAAAG AGATCCAGGA CCTACAGAGT CGCCAGAAAC ATGAAATCGA GTCTTTATAT 
ACCAAATTGG GCAAAGTCCC TCCTGCAGTC ATCATTCCCC CAGCTGCTCC ACTCTCAGGG 
AGAAGAAGAC GACCCACTAA AAGCAAAGGC AGCAAATCTA GCCGTAGTAG TTCTTTAGGA 
AATAAGAGCC CCCAACTTTC AGGTAACCTG TCTGCTCAGA GTGCAGCTTC AGTCTTGCAC 
CCCCAGCAGA CGCTCCACCC TCCAGGCAGC ATCCCAGAGA CTGGGCAGAA TCAGCTGTTA 
CAGCCCCTTA AACCATCTCC CTCCAGTGAG AACCTCTATT CAGCTTTTAC CAGTGATGGT 
GCCATTTCAG TACCAAGCCT TTCTGCTCCA GGCCAAGGCT GTGCGAAGTT TAACTGTGCA 
TCTGAGCGGG TGACATTCAA ACCTGGTGGC AGGAGGACCC GATTTCTGAG TACGCCCTGC 
TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC 
AGTACGAATA CAGTGGGAGG AGCAGTCAAT AGCCAAGCCC CACAAGCCCA GCCGCCCAGT 
AGAAAGGGCA CATTTACAGA TGATCTACAC AAGTTGGTAG ACAACTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGGAG AGGAAGCAAG GGGCACATGA ACTATGAGGG TCCTGGAATG 
GCAAGGAAAT TTTCAGCACC TGGACAGCTC TGCATCTCCA TGACCTCTAA TCTGGGTGGT 
TCTGCCCCCA TTTCTGCAGC ATCAGCTACC TCTTTAGGTC ACTTCACCAA GGCTATGTGC 
CCCCCACAGC AGTACAGCTT TCCATCTGCC CCTTTCAGCA CTCAGTGGAG TGGGACAAGT 
GGCCCAGCAC CGCCACCCCT CAGCCAGTTC CAGCCTGTAG GGACTTCGTC CTTGCAGAGT 
TTCAACATCA GCAATTTGCA AAAATCCATC AGCAATCCTC CAGGTTCAAA CCTGAGGACA 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795396.1
CDS	1085..8350
Translation	MSGGSVEKQSGPAGPRFLSPPAVPPKNSSSSDTSVGEKLGAAGAEPGAGRTEEYRRRRHTMDKDSRGSAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPGPVAAATAAPPPTLAPEPHREEPLTAPAASPAAPQPPAPPAPADHPNGGPASAAGRDPAVKHAPTPAASNHVGSKEEPAAAAAAAAAASGSAGAREQEERSQQQDDNEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQPTSVVTPTSVAGAVPVVVPAQKASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGCAKFNCASERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_031939536.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939536.1

ORF Insert Sequence:

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGCCC 
ACATCAGTTG TGACTCCAAC ATCAGTTGCT GGAGCAGTGC CAGTGGTAGT GCCAGCTCAG 
AAAGCTTCAA GCATAGAAGA TAATACCATG ATGACCAACT CAGAGGCTAG CGTTCTTAAA 
ATGGGCCGGT TTCAGGTATC AGTTGCAGTA GATGATTCCT TGAAGGAAAA TAGAAGCAAG 
TCGGAAGAAA TCAAGTCTGT ACACTTTGAA TCAAGTACTT CAGAGTCCTC AGTGCTGTCC 
AGTAGTAGCC CAGAGAATAC ACTGGTGAAG CAGGAGCCTG GTGGAGTCAC CACAGCTCTA 
TGTAGCCCCT CTTTGGATAT GGTTGACGGT GTTCCTTCTC AAAAAACCTC TGCTACCCAG 
ACAAAGCCCG AAGCTGAGCA GCCTGCCACA GTGGTTGGCC GATTCTCTGT GTCAAAAACT 
GAAGATAAGG TCACCCCTGT GGTGGAGGGA GAGCCACCTG TAGTACGCCC TTTATTGGAT 
TCTGAACAGG CCGTTCCTTC TGCTGTAGCA CCAAAGAAAG AAATGCCTGA ATCGCCAGAG 
CCAAGCCATC TTAATGGGCC ATCTTGTGAC CTAGAAGCTG CCTTTCTCAG TAGGAATATG 
GATGATGGTT CAGGTAGCCC CCATTCTACT CACCATTTGG GCTCAAAAGG CAGCCTTCCC 
ATTCAGAGCC TTAGCCAGAG CCTTAGCAAT TCATTCAACT CCTCTTACAT GAGCAGTGAC 
AATGAGTCAG ACATTGAAGA TGAGGACTTG AAGTCAGAAC TACGTCGGCT ACGAGAAAAG 
CACCTTAAAG AGATCCAGGA CCTACAGAGT CGCCAGAAAC ATGAAATCGA GTCTTTATAT 
ACCAAATTGG GCAAAGTCCC TCCTGCAGTC ATCATTCCCC CAGCTGCTCC ACTCTCAGGG 
AGAAGAAGAC GACCCACTAA AAGCAAAGGC AGCAAATCTA GCCGTAGTAG TTCTTTAGGA 
AATAAGAGCC CCCAACTTTC AGGTAACCTG TCTGCTCAGA GTGCAGCTTC AGTCTTGCAC 
CCCCAGCAGA CGCTCCACCC TCCAGGCAGC ATCCCAGAGA CTGGGCAGAA TCAGCTGTTA 
CAGCCCCTTA AACCATCTCC CTCCAGTGAG AACCTCTATT CAGCTTTTAC CAGTGATGGT 
GCCATTTCAG TACCAAGCCT TTCTGCTCCA GGCCAAGGCT GTGCGAAGTT TAACTGTGCA 
TCTGAGCGGG TGACATTCAA ACCTGGTGGC AGGAGGACCC GATTTCTGAG TACGCCCTGC 
TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGTCCTTGCA ACCAGCTCTG TAGGACCAGC 
AGTACGAATA CAGTGGGAGG AGCAGTCAAT AGCCAAGCCC CACAAGCCCA GCCGCCCAGT 
AGAAAGGGCA CATTTACAGA TGATCTACAC AAGTTGGTAG ACAACTGGGC CCGAGATGCC 
ATGAATCTCT CAGGCAGGAG AGGAAGCAAG GGGCACATGA ACTATGAGGG TCCTGGAATG 
GCAAGGAAAT TTTCAGCACC TGGACAGCTC TGCATCTCCA TGACCTCTAA TCTGGGTGGT 
TCTGCCCCCA TTTCTGCAGC ATCAGCTACC TCTTTAGGTC ACTTCACCAA GGCTATGTGC 
CCCCCACAGC AGTACAGCTT TCCATCTGCC CCTTTCAGCA CTCAGTGGAG TGGGACAAGT 
GGCCCAGCAC CGCCACCCCT CAGCCAGTTC CAGCCTGTAG GGACTTCGTC CTTGCAGAGT 
TTCAACATCA GCAATTTGCA AAAATCCATC AGCAATCCTC CAGGTTCAAA CCTGAGGACA 
ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939537.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795397.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

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CloneID	OSa237677
Clone ID Related Accession (Same CDS sequence)	XM_031939537.1
Accession Version	XM_031939537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGTACGAA TACAGTGGGA GGAGCAGTCA ATAGCCAAGC CCCACAAGCC 
CAGCCGCCCA GTAGAAAGGG CACATTTACA GATGATCTAC ACAAGTTGGT AGACAACTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGG AGAGGAAGCA AGGGGCACAT GAACTATGAG 
GGTCCTGGAA TGGCAAGGAA ATTTTCAGCA CCTGGACAGC TCTGCATCTC CATGACCTCT 
AATCTGGGTG GTTCTGCCCC CATTTCTGCA GCATCAGCTA CCTCTTTAGG TCACTTCACC 
AAGGCTATGT GCCCCCCACA GCAGTACAGC TTTCCATCTG CCCCTTTCAG CACTCAGTGG 
AGTGGGACAA GTGGCCCAGC ACCGCCACCC CTCAGCCAGT TCCAGCCTGT AGGGACTTCG 
TCCTTGCAGA GTTTCAACAT CAGCAATTTG CAAAAATCCA TCAGCAATCC TCCAGGTTCA 
AACCTGAGGA CAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795397.1
CDS	1084..8301
Translation	MSGGSVEKQSGPAGPRFLSPPAVPPKNSSSSDTSVGEKLGAAGAEPGAGRTEEYRRRRHTMDKDSRGSAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPGPVAAATAAPPPTLAPEPHREEPLTAPAASPAAPQPPAPPAPADHPNGGPASAAGRDPAVKHAPTPAASNHVGSKEEPAAAAAAAAAASGSAGAREQEERSQQQDDNEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_031939537.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939537.1

ORF Insert Sequence:

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGTACGAA TACAGTGGGA GGAGCAGTCA ATAGCCAAGC CCCACAAGCC 
CAGCCGCCCA GTAGAAAGGG CACATTTACA GATGATCTAC ACAAGTTGGT AGACAACTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGG AGAGGAAGCA AGGGGCACAT GAACTATGAG 
GGTCCTGGAA TGGCAAGGAA ATTTTCAGCA CCTGGACAGC TCTGCATCTC CATGACCTCT 
AATCTGGGTG GTTCTGCCCC CATTTCTGCA GCATCAGCTA CCTCTTTAGG TCACTTCACC 
AAGGCTATGT GCCCCCCACA GCAGTACAGC TTTCCATCTG CCCCTTTCAG CACTCAGTGG 
AGTGGGACAA GTGGCCCAGC ACCGCCACCC CTCAGCCAGT TCCAGCCTGT AGGGACTTCG 
TCCTTGCAGA GTTTCAACAT CAGCAATTTG CAAAAATCCA TCAGCAATCC TCCAGGTTCA 
AACCTGAGGA CAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939538.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795398.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

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CloneID	OSa237678
Clone ID Related Accession (Same CDS sequence)	XM_031939538.1
Accession Version	XM_031939538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGCTGTG CGAAGTTTAA CTGTGCATCT GAGCGGGTGA CATTCAAACC TGGTGGCAGG 
AGGACCCGAT TTCTGAGTAC GCCCTGCTTG GCTCTTTGGA AGATGGTGAA AAAAGTCTGT 
CCTTGCAACC AGCTCTGTAT GTGCCCTCCA GCAGAGCCGA AATCAGGCAG GACACAGACT 
CCTTCTTTCT CATCAATCAT ACAAATAAAA TTACCTTCTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795398.1
CDS	1086..8030
Translation	MSGGSVEKQSGPAGPRFLSPPAVPPKNSSSSDTSVGEKLGAAGAEPGAGRTEEYRRRRHTMDKDSRGSAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPGPVAAATAAPPPTLAPEPHREEPLTAPAASPAAPQPPAPPAPADHPNGGPASAAGRDPAVKHAPTPAASNHVGSKEEPAAAAAAAAAASGSAGAREQEERSQQQDDNEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGCAKFNCASERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCMCPPAEPKSGRTQTPSFSSIIQIKLPSP

Target ORF information:

RefSeq Version	XM_031939538.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939538.1

ORF Insert Sequence:

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGCTGTG CGAAGTTTAA CTGTGCATCT GAGCGGGTGA CATTCAAACC TGGTGGCAGG 
AGGACCCGAT TTCTGAGTAC GCCCTGCTTG GCTCTTTGGA AGATGGTGAA AAAAGTCTGT 
CCTTGCAACC AGCTCTGTAT GTGCCCTCCA GCAGAGCCGA AATCAGGCAG GACACAGACT 
CCTTCTTTCT CATCAATCAT ACAAATAAAA TTACCTTCTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939539.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795399.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

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CloneID	OSa237679
Clone ID Related Accession (Same CDS sequence)	XM_031939539.1
Accession Version	XM_031939539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGCTGTG CGAAGTTTAA CTGTGCATCT GAGCGGGTGA CATTCAAACC TGGTGGCAGG 
AGGACCCGAT TTCTGAGGAA GATGGTGAAA AAAGTCTGTC CTTGCAACCA GCTCTGTATG 
TGCCCTCCAG CAGAGCCGAA ATCAGGCAGG ACACAGACTC CTTCTTTCTC ATCAATCATA 
CAAATAAAAT TACCTTCTCC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795399.1
CDS	1086..8009
Translation	MSGGSVEKQSGPAGPRFLSPPAVPPKNSSSSDTSVGEKLGAAGAEPGAGRTEEYRRRRHTMDKDSRGSAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPGPVAAATAAPPPTLAPEPHREEPLTAPAASPAAPQPPAPPAPADHPNGGPASAAGRDPAVKHAPTPAASNHVGSKEEPAAAAAAAAAASGSAGAREQEERSQQQDDNEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGCAKFNCASERVTFKPGGRRTRFLRKMVKKVCPCNQLCMCPPAEPKSGRTQTPSFSSIIQIKLPSP

Target ORF information:

RefSeq Version	XM_031939539.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939539.1

ORF Insert Sequence:

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGCTGTG CGAAGTTTAA CTGTGCATCT GAGCGGGTGA CATTCAAACC TGGTGGCAGG 
AGGACCCGAT TTCTGAGGAA GATGGTGAAA AAAGTCTGTC CTTGCAACCA GCTCTGTATG 
TGCCCTCCAG CAGAGCCGAA ATCAGGCAGG ACACAGACTC CTTCTTTCTC ATCAATCATA 
CAAATAAAAT TACCTTCTCC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939540.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795400.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

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CloneID	OSa237680
Clone ID Related Accession (Same CDS sequence)	XM_031939540.1
Accession Version	XM_031939540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGGGA 
TTCCCACCTC GACTGCCACC TCAATACCCA GGAGACTCAA ATATTATTCC CTCATCCACC 
GTGGCTTCTG TTTGCGTCCC TTCTACAGTT CTGTCCCCTC CCTTGCCCAC AGAAGCATTG 
GCTCCACCAG GGTACCTTCC TACAGTTGTG CAGCCATATG TCGAGCCAAG TGTTTTAGTT 
CCTGTGGGTA GTCTAGGAGG ACAGGTTCAA GTACCCCATT CAGCAGTCAC CTTAGCACAG 
CAGGCCCCCT CTGCACCCTC CCAGCCAGCA GCTCTGGAGA CACAAGCAGT TTCCCAGTCC 
AGTCCTCCAG AACCTGTGCC ATCAGCACAG TCACAGACCA CTACATTGGT GTCCTCTATA 
GATAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GGAATGAGAA TGTTCCTTCA 
TCCAGTGGGA GACATGAAGG GAGAACTACA AAACGCCATT ACCGAAAGTC TGTACGGAGT 
CGTTCTCGTC ATGAGAAGAC TGCTCGCCCC AAATTGAGAA TTCTTAATGT GTCAAACAAA 
GGAGACAGAG TGGTGGAATG CCAGTTAGAG ACTCACAACC GGAAAATGGT TACATTCAAA 
TTTGACTTGG ATGGTGACAA TCCTGAGGAG ATAGCATCGA TTATGGTGAA CAATGATTTT 
ATTCTAGCAA CAGAGAGAGA CTCCTTTGTG GATCAGGTGC GAGAAATCAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTTAGTGTG GAACCAGAAG GTGATCAAGG ATCAGAAAGC 
TTACAAACAA AGGATGATTA TGGCTTTTCA GGATCCCAGA AATTGGAGGG AGAGTTCAAG 
CAACCAAATC CTGCATCTTC TATGCCTCAG AGGGCAGGTG TTCCCTCCAG TTCATTAACT 
CAAGTTGTAC ATTCAGCTGG ACGGAGGTTC ATAGTGAGTC CTGTACCAGA GAGTCGGTTA 
CGAGAGTCAG TATTTTTCAC TGCTACATCA AGTGAAATAT CAGACACAAC TGCTGTTGCT 
CCATCCCATG GCCCTGTAAT GAATCTGTCT CATTCTGCTT CATCCGTTAG TTTGCAACAG 
GCATTCTCTG AACTTAGACA TGCTCAAATG TCAGAAGGAC CCAGTACAGC TCCCCCAAAC 
TTCAGCCATG CAGGACCAAC CTTTCCAACT GTACCTGCCA CTATGACTAG CATGGCTGTA 
GTCTTCCCTC CCACGGCAAC AGCTGTGCCT TCAGTATCTG TCCCTGCTAC AAGTAGCCCT 
TTTAATAGTG TTTCTGCACC AGTCATTCAA TCTGTGGTCA CTATGCCTAC TGAAAAGGTA 
ATTGCCGGAG TTGCCTCCAG TGCAGGTGTA TTAACTTCAA GTTCTTTTCC TGTACCACAG 
GTGTCTGGAC CACCAGTAAT GACCAGTGTG GTTTCCAGCA TTACTATTCC TGCAGCCGTG 
TCATTGTCTA CTGCAAGTGA ATCAGTTCAG GCCCCACCAG TTGGGGGAGT TATGTCTAGT 
GCTGGCACTT TTCCTCCTGC AGCGGGATTG GTAGTTCCAG TTCCTGGAAT AGCCAGTACA 
GTTCCCCCAG GTGCTATCAC ACCAGCTATG ACTTCTCAGC CCACCCCAAT TGCAACAAAT 
ATAACTGGGG CAGCCACCCT GACTTCCACA TCTGCCACCA CTCCGTTCCC AAGCATGGCT 
TCACAACCAC CTCTTCAAAT CAGTAGCAGC AGCTCTGCTC CCACTGTAGC AGAAACTGTG 
GTAGTCAGTG CACTCCATCC CAAACACGAT GGCACACATT TACCAGACAA GCCACCCCTT 
AGCAGTGCAG CAGGGCTCTC CCTGCCCCTC TCAGTAGCTC CATCTTCCTC TGGAGCTGGG 
ATCTCTGATT CAGCTAGTCA GCCCAGTGTG CTGCATCCTT TACAGGTCCC ATCCGCTGTG 
GCTTCTCCTC CAGTACTTTC ACAGGCAGCA GGGACCGTCT CCACCCCGGT GTTACCTCAG 
GTACCTTTAC CTGGTATCCC GCCCCTAGCA CAGCCTATTA CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA ACCTCAACCA GCTTCACTTC CCAACCAGCC CCACACTCAT 
TGCCCTGAAG TAGATACTGA TACACAACCC AAAGCCCCTG GCATTGATGA TATAAAGACT 
CTGGAAGAAA AACTGCGGTC TCTCTTCAGT GAACACAGTT CTGTTGGAGC CCAGCATGCC 
CCTGTCTCCC TGGAGACATC ATTGGTAGTG GAGACTACAG TCATGCCAGG CATCCCAACC 
ACCGCTGTGG CACCAAGCAA GCTCATGACT TCCACCACGA GCACTTGCTT ACCACCAAGC 
AGTTTGCCTC TAGGAACAAC TACTGGTTTG TCAGCTGTTA CACCAGTGGT CACACCCGGG 
CAGGCTGCCA CCCCAGTCAG CTATGTTCCA GCCCCCACCA GCACTGCAGT AGGGGTGAAA 
CCTGGAACCC CTCCCTCAAA GCCACCTATC GCCAGGGTAC CGGTACAACC AGTAGGTACT 
GAACTTCAAT CTGGTACCCC ACCCAGCGAG CAGCTGCCGC CTTTTCCAGG ACCTTCCCTA 
ACACAGTCTC AGCAACCTCT AGAAGATCTT GATGCTCAGT TAAGAAGAGC CCTTAGTCCA 
GAGACTGTCC CAGTCACATC TTCAGTTGCT CCCACATCAG TTGTGACTCC AACATCAGTT 
GCTGGAGCAG TGCCAGTGGT AGTGCCAGCT CAGAAAGCAG CTTCAAGCAT AGAAGATAAT 
ACCATGATGA CCAACTCAGA GGCTAGCGTT CTTAAAATGG GCCGGTTTCA GGTATCAGTT 
GCAGTAGATG ATTCCTTGAA GGAAAATAGA AGCAAGTCGG AAGAAATCAA GTCTGTACAC 
TTTGAATCAA GTACTTCAGA GTCCTCAGTG CTGTCCAGTA GTAGCCCAGA GAATACACTG 
GTGAAGCAGG AGCCTGGTGG AGTCACCACA GCTCTATGTA GCCCCTCTTT GGATATGGTT 
GACGGTGTTC CTTCTCAAAA AACCTCTGCT ACCCAGACAA AGCCCGAAGC TGAGCAGCCT 
GCCACAGTGG TTGGCCGATT CTCTGTGTCA AAAACTGAAG ATAAGGTCAC CCCTGTGGTG 
GAGGGAGAGC CACCTGTAGT ACGCCCTTTA TTGGATTCTG AACAGGCCGT TCCTTCTGCT 
GTAGCACCAA AGAAAGAAAT GCCTGAATCG CCAGAGCCAA GCCATCTTAA TGGGCCATCT 
TGTGACCTAG AAGCTGCCTT TCTCAGTAGG AATATGGATG ATGGTTCAGG TAGCCCCCAT 
TCTACTCACC ATTTGGGCTC AAAAGGCAGC CTTCCCATTC AGAGCCTTAG CCAGAGCCTT 
AGCAATTCAT TCAACTCCTC TTACATGAGC AGTGACAATG AGTCAGACAT TGAAGATGAG 
GACTTGAAGT CAGAACTACG TCGGCTACGA GAAAAGCACC TTAAAGAGAT CCAGGACCTA 
CAGAGTCGCC AGAAACATGA AATCGAGTCT TTATATACCA AATTGGGCAA AGTCCCTCCT 
GCAGTCATCA TTCCCCCAGC TGCTCCACTC TCAGGGAGAA GAAGACGACC CACTAAAAGC 
AAAGGCAGCA AATCTAGCCG TAGTAGTTCT TTAGGAAATA AGAGCCCCCA ACTTTCAGGT 
AACCTGTCTG CTCAGAGTGC AGCTTCAGTC TTGCACCCCC AGCAGACGCT CCACCCTCCA 
GGCAGCATCC CAGAGACTGG GCAGAATCAG CTGTTACAGC CCCTTAAACC ATCTCCCTCC 
AGTGAGAACC TCTATTCAGC TTTTACCAGT GATGGTGCCA TTTCAGTACC AAGCCTTTCT 
GCTCCAGGCC AAGGCTGTGC GAAGTTTAAC TGTGCATCTG AGCGGGTGAC ATTCAAACCT 
GGTGGCAGGA GGACCCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA 
AAAGTCTGTC CTTGCAACCA GCTCTGTAGG ACCAGCAGTA CGAATACAGT GGGAGGAGCA 
GTCAATAGCC AAGCCCCACA AGCCCAGCCG CCCAGTAGAA AGGGCACATT TACAGATGAT 
CTACACAAGT TGGTAGACAA CTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGGAGAGGA 
AGCAAGGGGC ACATGAACTA TGAGGGTCCT GGAATGGCAA GGAAATTTTC AGCACCTGGA 
CAGCTCTGCA TCTCCATGAC CTCTAATCTG GGTGGTTCTG CCCCCATTTC TGCAGCATCA 
GCTACCTCTT TAGGTCACTT CACCAAGGCT ATGTGCCCCC CACAGCAGTA CAGCTTTCCA 
TCTGCCCCTT TCAGCACTCA GTGGAGTGGG ACAAGTGGCC CAGCACCGCC ACCCCTCAGC 
CAGTTCCAGC CTGTAGGGAC TTCGTCCTTG CAGAGTTTCA ACATCAGCAA TTTGCAAAAA 
TCCATCAGCA ATCCTCCAGG TTCAAACCTG AGGACAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795400.1
CDS	1084..7965
Translation	MSGGSVEKQSGPAGPRFLSPPAVPPKNSSSSDTSVGEKLGAAGAEPGAGRTEEYRRRRHTMDKDSRGSAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPGPVAAATAAPPPTLAPEPHREEPLTAPAASPAAPQPPAPPAPADHPNGGPASAAGRDPAVKHAPTPAASNHVGSKEEPAAAAAAAAAASGSAGAREQEERSQQQDDNEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGCAKFNCASERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_031939540.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939540.1

ORF Insert Sequence:

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGGGA 
TTCCCACCTC GACTGCCACC TCAATACCCA GGAGACTCAA ATATTATTCC CTCATCCACC 
GTGGCTTCTG TTTGCGTCCC TTCTACAGTT CTGTCCCCTC CCTTGCCCAC AGAAGCATTG 
GCTCCACCAG GGTACCTTCC TACAGTTGTG CAGCCATATG TCGAGCCAAG TGTTTTAGTT 
CCTGTGGGTA GTCTAGGAGG ACAGGTTCAA GTACCCCATT CAGCAGTCAC CTTAGCACAG 
CAGGCCCCCT CTGCACCCTC CCAGCCAGCA GCTCTGGAGA CACAAGCAGT TTCCCAGTCC 
AGTCCTCCAG AACCTGTGCC ATCAGCACAG TCACAGACCA CTACATTGGT GTCCTCTATA 
GATAGTGCAC ATTCAGATGT TGCTTCAGGT ATGAGTGATG GGAATGAGAA TGTTCCTTCA 
TCCAGTGGGA GACATGAAGG GAGAACTACA AAACGCCATT ACCGAAAGTC TGTACGGAGT 
CGTTCTCGTC ATGAGAAGAC TGCTCGCCCC AAATTGAGAA TTCTTAATGT GTCAAACAAA 
GGAGACAGAG TGGTGGAATG CCAGTTAGAG ACTCACAACC GGAAAATGGT TACATTCAAA 
TTTGACTTGG ATGGTGACAA TCCTGAGGAG ATAGCATCGA TTATGGTGAA CAATGATTTT 
ATTCTAGCAA CAGAGAGAGA CTCCTTTGTG GATCAGGTGC GAGAAATCAT TGAAAAAGCT 
GATGAAATGC TCAGTGAGGA TGTTAGTGTG GAACCAGAAG GTGATCAAGG ATCAGAAAGC 
TTACAAACAA AGGATGATTA TGGCTTTTCA GGATCCCAGA AATTGGAGGG AGAGTTCAAG 
CAACCAAATC CTGCATCTTC TATGCCTCAG AGGGCAGGTG TTCCCTCCAG TTCATTAACT 
CAAGTTGTAC ATTCAGCTGG ACGGAGGTTC ATAGTGAGTC CTGTACCAGA GAGTCGGTTA 
CGAGAGTCAG TATTTTTCAC TGCTACATCA AGTGAAATAT CAGACACAAC TGCTGTTGCT 
CCATCCCATG GCCCTGTAAT GAATCTGTCT CATTCTGCTT CATCCGTTAG TTTGCAACAG 
GCATTCTCTG AACTTAGACA TGCTCAAATG TCAGAAGGAC CCAGTACAGC TCCCCCAAAC 
TTCAGCCATG CAGGACCAAC CTTTCCAACT GTACCTGCCA CTATGACTAG CATGGCTGTA 
GTCTTCCCTC CCACGGCAAC AGCTGTGCCT TCAGTATCTG TCCCTGCTAC AAGTAGCCCT 
TTTAATAGTG TTTCTGCACC AGTCATTCAA TCTGTGGTCA CTATGCCTAC TGAAAAGGTA 
ATTGCCGGAG TTGCCTCCAG TGCAGGTGTA TTAACTTCAA GTTCTTTTCC TGTACCACAG 
GTGTCTGGAC CACCAGTAAT GACCAGTGTG GTTTCCAGCA TTACTATTCC TGCAGCCGTG 
TCATTGTCTA CTGCAAGTGA ATCAGTTCAG GCCCCACCAG TTGGGGGAGT TATGTCTAGT 
GCTGGCACTT TTCCTCCTGC AGCGGGATTG GTAGTTCCAG TTCCTGGAAT AGCCAGTACA 
GTTCCCCCAG GTGCTATCAC ACCAGCTATG ACTTCTCAGC CCACCCCAAT TGCAACAAAT 
ATAACTGGGG CAGCCACCCT GACTTCCACA TCTGCCACCA CTCCGTTCCC AAGCATGGCT 
TCACAACCAC CTCTTCAAAT CAGTAGCAGC AGCTCTGCTC CCACTGTAGC AGAAACTGTG 
GTAGTCAGTG CACTCCATCC CAAACACGAT GGCACACATT TACCAGACAA GCCACCCCTT 
AGCAGTGCAG CAGGGCTCTC CCTGCCCCTC TCAGTAGCTC CATCTTCCTC TGGAGCTGGG 
ATCTCTGATT CAGCTAGTCA GCCCAGTGTG CTGCATCCTT TACAGGTCCC ATCCGCTGTG 
GCTTCTCCTC CAGTACTTTC ACAGGCAGCA GGGACCGTCT CCACCCCGGT GTTACCTCAG 
GTACCTTTAC CTGGTATCCC GCCCCTAGCA CAGCCTATTA CCAATGTGCC TGCTGTACAG 
CAGACACTAA TTCATAGTCA ACCTCAACCA GCTTCACTTC CCAACCAGCC CCACACTCAT 
TGCCCTGAAG TAGATACTGA TACACAACCC AAAGCCCCTG GCATTGATGA TATAAAGACT 
CTGGAAGAAA AACTGCGGTC TCTCTTCAGT GAACACAGTT CTGTTGGAGC CCAGCATGCC 
CCTGTCTCCC TGGAGACATC ATTGGTAGTG GAGACTACAG TCATGCCAGG CATCCCAACC 
ACCGCTGTGG CACCAAGCAA GCTCATGACT TCCACCACGA GCACTTGCTT ACCACCAAGC 
AGTTTGCCTC TAGGAACAAC TACTGGTTTG TCAGCTGTTA CACCAGTGGT CACACCCGGG 
CAGGCTGCCA CCCCAGTCAG CTATGTTCCA GCCCCCACCA GCACTGCAGT AGGGGTGAAA 
CCTGGAACCC CTCCCTCAAA GCCACCTATC GCCAGGGTAC CGGTACAACC AGTAGGTACT 
GAACTTCAAT CTGGTACCCC ACCCAGCGAG CAGCTGCCGC CTTTTCCAGG ACCTTCCCTA 
ACACAGTCTC AGCAACCTCT AGAAGATCTT GATGCTCAGT TAAGAAGAGC CCTTAGTCCA 
GAGACTGTCC CAGTCACATC TTCAGTTGCT CCCACATCAG TTGTGACTCC AACATCAGTT 
GCTGGAGCAG TGCCAGTGGT AGTGCCAGCT CAGAAAGCAG CTTCAAGCAT AGAAGATAAT 
ACCATGATGA CCAACTCAGA GGCTAGCGTT CTTAAAATGG GCCGGTTTCA GGTATCAGTT 
GCAGTAGATG ATTCCTTGAA GGAAAATAGA AGCAAGTCGG AAGAAATCAA GTCTGTACAC 
TTTGAATCAA GTACTTCAGA GTCCTCAGTG CTGTCCAGTA GTAGCCCAGA GAATACACTG 
GTGAAGCAGG AGCCTGGTGG AGTCACCACA GCTCTATGTA GCCCCTCTTT GGATATGGTT 
GACGGTGTTC CTTCTCAAAA AACCTCTGCT ACCCAGACAA AGCCCGAAGC TGAGCAGCCT 
GCCACAGTGG TTGGCCGATT CTCTGTGTCA AAAACTGAAG ATAAGGTCAC CCCTGTGGTG 
GAGGGAGAGC CACCTGTAGT ACGCCCTTTA TTGGATTCTG AACAGGCCGT TCCTTCTGCT 
GTAGCACCAA AGAAAGAAAT GCCTGAATCG CCAGAGCCAA GCCATCTTAA TGGGCCATCT 
TGTGACCTAG AAGCTGCCTT TCTCAGTAGG AATATGGATG ATGGTTCAGG TAGCCCCCAT 
TCTACTCACC ATTTGGGCTC AAAAGGCAGC CTTCCCATTC AGAGCCTTAG CCAGAGCCTT 
AGCAATTCAT TCAACTCCTC TTACATGAGC AGTGACAATG AGTCAGACAT TGAAGATGAG 
GACTTGAAGT CAGAACTACG TCGGCTACGA GAAAAGCACC TTAAAGAGAT CCAGGACCTA 
CAGAGTCGCC AGAAACATGA AATCGAGTCT TTATATACCA AATTGGGCAA AGTCCCTCCT 
GCAGTCATCA TTCCCCCAGC TGCTCCACTC TCAGGGAGAA GAAGACGACC CACTAAAAGC 
AAAGGCAGCA AATCTAGCCG TAGTAGTTCT TTAGGAAATA AGAGCCCCCA ACTTTCAGGT 
AACCTGTCTG CTCAGAGTGC AGCTTCAGTC TTGCACCCCC AGCAGACGCT CCACCCTCCA 
GGCAGCATCC CAGAGACTGG GCAGAATCAG CTGTTACAGC CCCTTAAACC ATCTCCCTCC 
AGTGAGAACC TCTATTCAGC TTTTACCAGT GATGGTGCCA TTTCAGTACC AAGCCTTTCT 
GCTCCAGGCC AAGGCTGTGC GAAGTTTAAC TGTGCATCTG AGCGGGTGAC ATTCAAACCT 
GGTGGCAGGA GGACCCGATT TCTGAGTACG CCCTGCTTGG CTCTTTGGAA GATGGTGAAA 
AAAGTCTGTC CTTGCAACCA GCTCTGTAGG ACCAGCAGTA CGAATACAGT GGGAGGAGCA 
GTCAATAGCC AAGCCCCACA AGCCCAGCCG CCCAGTAGAA AGGGCACATT TACAGATGAT 
CTACACAAGT TGGTAGACAA CTGGGCCCGA GATGCCATGA ATCTCTCAGG CAGGAGAGGA 
AGCAAGGGGC ACATGAACTA TGAGGGTCCT GGAATGGCAA GGAAATTTTC AGCACCTGGA 
CAGCTCTGCA TCTCCATGAC CTCTAATCTG GGTGGTTCTG CCCCCATTTC TGCAGCATCA 
GCTACCTCTT TAGGTCACTT CACCAAGGCT ATGTGCCCCC CACAGCAGTA CAGCTTTCCA 
TCTGCCCCTT TCAGCACTCA GTGGAGTGGG ACAAGTGGCC CAGCACCGCC ACCCCTCAGC 
CAGTTCCAGC CTGTAGGGAC TTCGTCCTTG CAGAGTTTCA ACATCAGCAA TTTGCAAAAA 
TCCATCAGCA ATCCTCCAGG TTCAAACCTG AGGACAACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939541.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795401.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

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CloneID	OSa237681
Clone ID Related Accession (Same CDS sequence)	XM_031939541.1
Accession Version	XM_031939541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGACA 
CAAGCAGTTT CCCAGTCCAG TCCTCCAGAA CCTGTGCCAT CAGCACAGTC ACAGACCACT 
ACATTGGTGT CCTCTATAGA TAGTGCACAT TCAGATGTTG CTTCAGGTAT GAGTGATGGG 
AATGAGAATG TTCCTTCATC CAGTGGGAGA CATGAAGGGA GAACTACAAA ACGCCATTAC 
CGAAAGTCTG TACGGAGTCG TTCTCGTCAT GAGAAGACTG CTCGCCCCAA ATTGAGAATT 
CTTAATGTGT CAAACAAAGG AGACAGAGTG GTGGAATGCC AGTTAGAGAC TCACAACCGG 
AAAATGGTTA CATTCAAATT TGACTTGGAT GGTGACAATC CTGAGGAGAT AGCATCGATT 
ATGGTGAACA ATGATTTTAT TCTAGCAACA GAGAGAGACT CCTTTGTGGA TCAGGTGCGA 
GAAATCATTG AAAAAGCTGA TGAAATGCTC AGTGAGGATG TTAGTGTGGA ACCAGAAGGT 
GATCAAGGAT CAGAAAGCTT ACAAACAAAG GATGATTATG GCTTTTCAGG ATCCCAGAAA 
TTGGAGGGAG AGTTCAAGCA ACCAAATCCT GCATCTTCTA TGCCTCAGAG GGCAGGTGTT 
CCCTCCAGTT CATTAACTCA AGTTGTACAT TCAGCTGGAC GGAGGTTCAT AGTGAGTCCT 
GTACCAGAGA GTCGGTTACG AGAGTCAGTA TTTTTCACTG CTACATCAAG TGAAATATCA 
GACACAACTG CTGTTGCTCC ATCCCATGGC CCTGTAATGA ATCTGTCTCA TTCTGCTTCA 
TCCGTTAGTT TGCAACAGGC ATTCTCTGAA CTTAGACATG CTCAAATGTC AGAAGGACCC 
AGTACAGCTC CCCCAAACTT CAGCCATGCA GGACCAACCT TTCCAACTGT ACCTGCCACT 
ATGACTAGCA TGGCTGTAGT CTTCCCTCCC ACGGCAACAG CTGTGCCTTC AGTATCTGTC 
CCTGCTACAA GTAGCCCTTT TAATAGTGTT TCTGCACCAG TCATTCAATC TGTGGTCACT 
ATGCCTACTG AAAAGGTAAT TGCCGGAGTT GCCTCCAGTG CAGGTGTATT AACTTCAAGT 
TCTTTTCCTG TACCACAGGT GTCTGGACCA CCAGTAATGA CCAGTGTGGT TTCCAGCATT 
ACTATTCCTG CAGCCGTGTC ATTGTCTACT GCAAGTGAAT CAGTTCAGGC CCCACCAGTT 
GGGGGAGTTA TGTCTAGTGC TGGCACTTTT CCTCCTGCAG CGGGATTGGT AGTTCCAGTT 
CCTGGAATAG CCAGTACAGT TCCCCCAGGT GCTATCACAC CAGCTATGAC TTCTCAGCCC 
ACCCCAATTG CAACAAATAT AACTGGGGCA GCCACCCTGA CTTCCACATC TGCCACCACT 
CCGTTCCCAA GCATGGCTTC ACAACCACCT CTTCAAATCA GTAGCAGCAG CTCTGCTCCC 
ACTGTAGCAG AAACTGTGGT AGTCAGTGCA CTCCATCCCA AACACGATGG CACACATTTA 
CCAGACAAGC CACCCCTTAG CAGTGCAGCA GGGCTCTCCC TGCCCCTCTC AGTAGCTCCA 
TCTTCCTCTG GAGCTGGGAT CTCTGATTCA GCTAGTCAGC CCAGTGTGCT GCATCCTTTA 
CAGGTCCCAT CCGCTGTGGC TTCTCCTCCA GTACTTTCAC AGGCAGCAGG GACCGTCTCC 
ACCCCGGTGT TACCTCAGGT ACCTTTACCT GGTATCCCGC CCCTAGCACA GCCTATTACC 
AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAAC CTCAACCAGC TTCACTTCCC 
AACCAGCCCC ACACTCATTG CCCTGAAGTA GATACTGATA CACAACCCAA AGCCCCTGGC 
ATTGATGATA TAAAGACTCT GGAAGAAAAA CTGCGGTCTC TCTTCAGTGA ACACAGTTCT 
GTTGGAGCCC AGCATGCCCC TGTCTCCCTG GAGACATCAT TGGTAGTGGA GACTACAGTC 
ATGCCAGGCA TCCCAACCAC CGCTGTGGCA CCAAGCAAGC TCATGACTTC CACCACGAGC 
ACTTGCTTAC CACCAAGCAG TTTGCCTCTA GGAACAACTA CTGGTTTGTC AGCTGTTACA 
CCAGTGGTCA CACCCGGGCA GGCTGCCACC CCAGTCAGCT ATGTTCCAGC CCCCACCAGC 
ACTGCAGTAG GGGTGAAACC TGGAACCCCT CCCTCAAAGC CACCTATCGC CAGGGTACCG 
GTACAACCAG TAGGTACTGA ACTTCAATCT GGTACCCCAC CCAGCGAGCA GCTGCCGCCT 
TTTCCAGGAC CTTCCCTAAC ACAGTCTCAG CAACCTCTAG AAGATCTTGA TGCTCAGTTA 
AGAAGAGCCC TTAGTCCAGA GACTGTCCCA GTCACATCTT CAGTTGCTCC CACATCAGTT 
GTGACTCCAA CATCAGTTGC TGGAGCAGTG CCAGTGGTAG TGCCAGCTCA GAAAGCAGCT 
TCAAGCATAG AAGATAATAC CATGATGACC AACTCAGAGG CTAGCGTTCT TAAAATGGGC 
CGGTTTCAGG TATCAGTTGC AGTAGATGAT TCCTTGAAGG AAAATAGAAG CAAGTCGGAA 
GAAATCAAGT CTGTACACTT TGAATCAAGT ACTTCAGAGT CCTCAGTGCT GTCCAGTAGT 
AGCCCAGAGA ATACACTGGT GAAGCAGGAG CCTGGTGGAG TCACCACAGC TCTATGTAGC 
CCCTCTTTGG ATATGGTTGA CGGTGTTCCT TCTCAAAAAA CCTCTGCTAC CCAGACAAAG 
CCCGAAGCTG AGCAGCCTGC CACAGTGGTT GGCCGATTCT CTGTGTCAAA AACTGAAGAT 
AAGGTCACCC CTGTGGTGGA GGGAGAGCCA CCTGTAGTAC GCCCTTTATT GGATTCTGAA 
CAGGCCGTTC CTTCTGCTGT AGCACCAAAG AAAGAAATGC CTGAATCGCC AGAGCCAAGC 
CATCTTAATG GGCCATCTTG TGACCTAGAA GCTGCCTTTC TCAGTAGGAA TATGGATGAT 
GGTTCAGGTA GCCCCCATTC TACTCACCAT TTGGGCTCAA AAGGCAGCCT TCCCATTCAG 
AGCCTTAGCC AGAGCCTTAG CAATTCATTC AACTCCTCTT ACATGAGCAG TGACAATGAG 
TCAGACATTG AAGATGAGGA CTTGAAGTCA GAACTACGTC GGCTACGAGA AAAGCACCTT 
AAAGAGATCC AGGACCTACA GAGTCGCCAG AAACATGAAA TCGAGTCTTT ATATACCAAA 
TTGGGCAAAG TCCCTCCTGC AGTCATCATT CCCCCAGCTG CTCCACTCTC AGGGAGAAGA 
AGACGACCCA CTAAAAGCAA AGGCAGCAAA TCTAGCCGTA GTAGTTCTTT AGGAAATAAG 
AGCCCCCAAC TTTCAGGTAA CCTGTCTGCT CAGAGTGCAG CTTCAGTCTT GCACCCCCAG 
CAGACGCTCC ACCCTCCAGG CAGCATCCCA GAGACTGGGC AGAATCAGCT GTTACAGCCC 
CTTAAACCAT CTCCCTCCAG TGAGAACCTC TATTCAGCTT TTACCAGTGA TGGTGCCATT 
TCAGTACCAA GCCTTTCTGC TCCAGGCCAA GGCTGTGCGA AGTTTAACTG TGCATCTGAG 
CGGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT 
CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGTACG 
AATACAGTGG GAGGAGCAGT CAATAGCCAA GCCCCACAAG CCCAGCCGCC CAGTAGAAAG 
GGCACATTTA CAGATGATCT ACACAAGTTG GTAGACAACT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GGAGAGGAAG CAAGGGGCAC ATGAACTATG AGGGTCCTGG AATGGCAAGG 
AAATTTTCAG CACCTGGACA GCTCTGCATC TCCATGACCT CTAATCTGGG TGGTTCTGCC 
CCCATTTCTG CAGCATCAGC TACCTCTTTA GGTCACTTCA CCAAGGCTAT GTGCCCCCCA 
CAGCAGTACA GCTTTCCATC TGCCCCTTTC AGCACTCAGT GGAGTGGGAC AAGTGGCCCA 
GCACCGCCAC CCCTCAGCCA GTTCCAGCCT GTAGGGACTT CGTCCTTGCA GAGTTTCAAC 
ATCAGCAATT TGCAAAAATC CATCAGCAAT CCTCCAGGTT CAAACCTGAG GACAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795401.1
CDS	1084..7683
Translation	MSGGSVEKQSGPAGPRFLSPPAVPPKNSSSSDTSVGEKLGAAGAEPGAGRTEEYRRRRHTMDKDSRGSAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPGPVAAATAAPPPTLAPEPHREEPLTAPAASPAAPQPPAPPAPADHPNGGPASAAGRDPAVKHAPTPAASNHVGSKEEPAAAAAAAAAASGSAGAREQEERSQQQDDNEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQTQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGCAKFNCASERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_031939541.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939541.1

ORF Insert Sequence:

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGACA 
CAAGCAGTTT CCCAGTCCAG TCCTCCAGAA CCTGTGCCAT CAGCACAGTC ACAGACCACT 
ACATTGGTGT CCTCTATAGA TAGTGCACAT TCAGATGTTG CTTCAGGTAT GAGTGATGGG 
AATGAGAATG TTCCTTCATC CAGTGGGAGA CATGAAGGGA GAACTACAAA ACGCCATTAC 
CGAAAGTCTG TACGGAGTCG TTCTCGTCAT GAGAAGACTG CTCGCCCCAA ATTGAGAATT 
CTTAATGTGT CAAACAAAGG AGACAGAGTG GTGGAATGCC AGTTAGAGAC TCACAACCGG 
AAAATGGTTA CATTCAAATT TGACTTGGAT GGTGACAATC CTGAGGAGAT AGCATCGATT 
ATGGTGAACA ATGATTTTAT TCTAGCAACA GAGAGAGACT CCTTTGTGGA TCAGGTGCGA 
GAAATCATTG AAAAAGCTGA TGAAATGCTC AGTGAGGATG TTAGTGTGGA ACCAGAAGGT 
GATCAAGGAT CAGAAAGCTT ACAAACAAAG GATGATTATG GCTTTTCAGG ATCCCAGAAA 
TTGGAGGGAG AGTTCAAGCA ACCAAATCCT GCATCTTCTA TGCCTCAGAG GGCAGGTGTT 
CCCTCCAGTT CATTAACTCA AGTTGTACAT TCAGCTGGAC GGAGGTTCAT AGTGAGTCCT 
GTACCAGAGA GTCGGTTACG AGAGTCAGTA TTTTTCACTG CTACATCAAG TGAAATATCA 
GACACAACTG CTGTTGCTCC ATCCCATGGC CCTGTAATGA ATCTGTCTCA TTCTGCTTCA 
TCCGTTAGTT TGCAACAGGC ATTCTCTGAA CTTAGACATG CTCAAATGTC AGAAGGACCC 
AGTACAGCTC CCCCAAACTT CAGCCATGCA GGACCAACCT TTCCAACTGT ACCTGCCACT 
ATGACTAGCA TGGCTGTAGT CTTCCCTCCC ACGGCAACAG CTGTGCCTTC AGTATCTGTC 
CCTGCTACAA GTAGCCCTTT TAATAGTGTT TCTGCACCAG TCATTCAATC TGTGGTCACT 
ATGCCTACTG AAAAGGTAAT TGCCGGAGTT GCCTCCAGTG CAGGTGTATT AACTTCAAGT 
TCTTTTCCTG TACCACAGGT GTCTGGACCA CCAGTAATGA CCAGTGTGGT TTCCAGCATT 
ACTATTCCTG CAGCCGTGTC ATTGTCTACT GCAAGTGAAT CAGTTCAGGC CCCACCAGTT 
GGGGGAGTTA TGTCTAGTGC TGGCACTTTT CCTCCTGCAG CGGGATTGGT AGTTCCAGTT 
CCTGGAATAG CCAGTACAGT TCCCCCAGGT GCTATCACAC CAGCTATGAC TTCTCAGCCC 
ACCCCAATTG CAACAAATAT AACTGGGGCA GCCACCCTGA CTTCCACATC TGCCACCACT 
CCGTTCCCAA GCATGGCTTC ACAACCACCT CTTCAAATCA GTAGCAGCAG CTCTGCTCCC 
ACTGTAGCAG AAACTGTGGT AGTCAGTGCA CTCCATCCCA AACACGATGG CACACATTTA 
CCAGACAAGC CACCCCTTAG CAGTGCAGCA GGGCTCTCCC TGCCCCTCTC AGTAGCTCCA 
TCTTCCTCTG GAGCTGGGAT CTCTGATTCA GCTAGTCAGC CCAGTGTGCT GCATCCTTTA 
CAGGTCCCAT CCGCTGTGGC TTCTCCTCCA GTACTTTCAC AGGCAGCAGG GACCGTCTCC 
ACCCCGGTGT TACCTCAGGT ACCTTTACCT GGTATCCCGC CCCTAGCACA GCCTATTACC 
AATGTGCCTG CTGTACAGCA GACACTAATT CATAGTCAAC CTCAACCAGC TTCACTTCCC 
AACCAGCCCC ACACTCATTG CCCTGAAGTA GATACTGATA CACAACCCAA AGCCCCTGGC 
ATTGATGATA TAAAGACTCT GGAAGAAAAA CTGCGGTCTC TCTTCAGTGA ACACAGTTCT 
GTTGGAGCCC AGCATGCCCC TGTCTCCCTG GAGACATCAT TGGTAGTGGA GACTACAGTC 
ATGCCAGGCA TCCCAACCAC CGCTGTGGCA CCAAGCAAGC TCATGACTTC CACCACGAGC 
ACTTGCTTAC CACCAAGCAG TTTGCCTCTA GGAACAACTA CTGGTTTGTC AGCTGTTACA 
CCAGTGGTCA CACCCGGGCA GGCTGCCACC CCAGTCAGCT ATGTTCCAGC CCCCACCAGC 
ACTGCAGTAG GGGTGAAACC TGGAACCCCT CCCTCAAAGC CACCTATCGC CAGGGTACCG 
GTACAACCAG TAGGTACTGA ACTTCAATCT GGTACCCCAC CCAGCGAGCA GCTGCCGCCT 
TTTCCAGGAC CTTCCCTAAC ACAGTCTCAG CAACCTCTAG AAGATCTTGA TGCTCAGTTA 
AGAAGAGCCC TTAGTCCAGA GACTGTCCCA GTCACATCTT CAGTTGCTCC CACATCAGTT 
GTGACTCCAA CATCAGTTGC TGGAGCAGTG CCAGTGGTAG TGCCAGCTCA GAAAGCAGCT 
TCAAGCATAG AAGATAATAC CATGATGACC AACTCAGAGG CTAGCGTTCT TAAAATGGGC 
CGGTTTCAGG TATCAGTTGC AGTAGATGAT TCCTTGAAGG AAAATAGAAG CAAGTCGGAA 
GAAATCAAGT CTGTACACTT TGAATCAAGT ACTTCAGAGT CCTCAGTGCT GTCCAGTAGT 
AGCCCAGAGA ATACACTGGT GAAGCAGGAG CCTGGTGGAG TCACCACAGC TCTATGTAGC 
CCCTCTTTGG ATATGGTTGA CGGTGTTCCT TCTCAAAAAA CCTCTGCTAC CCAGACAAAG 
CCCGAAGCTG AGCAGCCTGC CACAGTGGTT GGCCGATTCT CTGTGTCAAA AACTGAAGAT 
AAGGTCACCC CTGTGGTGGA GGGAGAGCCA CCTGTAGTAC GCCCTTTATT GGATTCTGAA 
CAGGCCGTTC CTTCTGCTGT AGCACCAAAG AAAGAAATGC CTGAATCGCC AGAGCCAAGC 
CATCTTAATG GGCCATCTTG TGACCTAGAA GCTGCCTTTC TCAGTAGGAA TATGGATGAT 
GGTTCAGGTA GCCCCCATTC TACTCACCAT TTGGGCTCAA AAGGCAGCCT TCCCATTCAG 
AGCCTTAGCC AGAGCCTTAG CAATTCATTC AACTCCTCTT ACATGAGCAG TGACAATGAG 
TCAGACATTG AAGATGAGGA CTTGAAGTCA GAACTACGTC GGCTACGAGA AAAGCACCTT 
AAAGAGATCC AGGACCTACA GAGTCGCCAG AAACATGAAA TCGAGTCTTT ATATACCAAA 
TTGGGCAAAG TCCCTCCTGC AGTCATCATT CCCCCAGCTG CTCCACTCTC AGGGAGAAGA 
AGACGACCCA CTAAAAGCAA AGGCAGCAAA TCTAGCCGTA GTAGTTCTTT AGGAAATAAG 
AGCCCCCAAC TTTCAGGTAA CCTGTCTGCT CAGAGTGCAG CTTCAGTCTT GCACCCCCAG 
CAGACGCTCC ACCCTCCAGG CAGCATCCCA GAGACTGGGC AGAATCAGCT GTTACAGCCC 
CTTAAACCAT CTCCCTCCAG TGAGAACCTC TATTCAGCTT TTACCAGTGA TGGTGCCATT 
TCAGTACCAA GCCTTTCTGC TCCAGGCCAA GGCTGTGCGA AGTTTAACTG TGCATCTGAG 
CGGGTGACAT TCAAACCTGG TGGCAGGAGG ACCCGATTTC TGAGTACGCC CTGCTTGGCT 
CTTTGGAAGA TGGTGAAAAA AGTCTGTCCT TGCAACCAGC TCTGTAGGAC CAGCAGTACG 
AATACAGTGG GAGGAGCAGT CAATAGCCAA GCCCCACAAG CCCAGCCGCC CAGTAGAAAG 
GGCACATTTA CAGATGATCT ACACAAGTTG GTAGACAACT GGGCCCGAGA TGCCATGAAT 
CTCTCAGGCA GGAGAGGAAG CAAGGGGCAC ATGAACTATG AGGGTCCTGG AATGGCAAGG 
AAATTTTCAG CACCTGGACA GCTCTGCATC TCCATGACCT CTAATCTGGG TGGTTCTGCC 
CCCATTTCTG CAGCATCAGC TACCTCTTTA GGTCACTTCA CCAAGGCTAT GTGCCCCCCA 
CAGCAGTACA GCTTTCCATC TGCCCCTTTC AGCACTCAGT GGAGTGGGAC AAGTGGCCCA 
GCACCGCCAC CCCTCAGCCA GTTCCAGCCT GTAGGGACTT CGTCCTTGCA GAGTTTCAAC 
ATCAGCAATT TGCAAAAATC CATCAGCAAT CCTCCAGGTT CAAACCTGAG GACAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939542.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795402.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

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CloneID	OSa237682
Clone ID Related Accession (Same CDS sequence)	XM_031939542.1
Accession Version	XM_031939542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG TAAAGCCAGC CAGCTTTGAC AAAGTAGCAA TTCCTGAAGT AAAGGAAATT 
ATTGAAGGAT GCATACGCCA AAACAAAGAT GAAAGATATG CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGAGTA CGAGTAGAAC TAGCTGAAGA AGATGATGGT 
GAAAAGATAG CCATTAAGCT ATGGTTACGC ATTGAAGATA TTAAGAAATT GAAGGGGAAA 
TACAAAGACA ATGAAGCAAT TGAATTTTCC TTTGATTTGG AGAGAGATGT CCCAGAAGAT 
GTAGCTCAAG AAATGGTAGA ATCAGGGTAT GTTTGTGAAG GAGATCACAA GACCATGGCC 
AAAGCCATCA AAGACAGAGT GTCCCTTATA AAGAGAAAAC GAGAGCAGCG ACAGTTGGTA 
AGGGAAGAAC AGGAGAAAAG AAAGCAAGAA GAGATCAGTT TAAAGGAGCA AGTTGAACAG 
CCAACAACCA TTTCTCAGGC TGGGACCAAA TTGATTCCTT CTACCCCTGG CACAGTTACT 
GTCTCTACCA CATCAGCCTC AGTTTCCACC CAAGTGGAGC CAGAAGAACC AGAGGCAGAT 
CAACACCAAC AACTGCAGTA TCAACAACCT AGTCTCTCCG TGTTGTCTGA TGGAACAGTT 
GACAGTGGTC AGGGGTCTTC AGTCTTCACA GAATCTCGAG TAAGCAGCCA GCAGACTGTC 
TCATATGGGT CTCAACATGA ACAGGCACTT TCAACAGGCA CGATCCCAAG CTATACAGCT 
TCTGTTGTTC AAGCACAATC CCAGCCTCAT GGTGTATATC CTTCCTCCAC CGTGACACCG 
GCACAGAGCC AGAGTCATCT GGCTTCAAGT AACTTAACTG GGATTCCATC TTCCCAGACA 
GCACAGCTTG CTCAGCAGGG TATACAACAA GCAGCCCCTC CTCAGCAGAC AGGCCAGTAT 
CTTCTTCCCC AGTCAGCAGC ATCTGGTGTG CCTACAACTG CACAGCCTGT GACGCAGCCC 
CAGCTTCCAC AGGTTCTGCC AATGCCTCAA GTATCAGTTG GGAAGCAGCT TCCTGTTTCT 
CAGCCAGTAT CGACCATCCA AGGCGAATCT CAGATACCAG TTGCAGCACC TTCCCTTCCA 
CAGCCCTCAG TTAACCCATC TCTCTCGGGT GCTCACTTCC TTCCTGTGGG CCAGCCACTT 
CCAGCATCCT TAATTCCTCA GTTCTCAGTC TCTCAGATTC CCATGTCAAC TCCTCATGTC 
TCTGTGGCTC AGACAGGTTT CTCATCCCTG CCAAGCTCTA TAGCATCTGG CATTAACCAG 
CCTCTGCTCA CCTTGGCCTC ATCTGCTGCA GCGGCTGCTA TCCCTGTGGG ATCAACTATG 
GTCCCTAGTC AGCTTCCCAC CCTTCTGCAG CCTGTGACTC AGCTGCCAAG TCAGGCTCAC 
CCACAGCTCC TTCAGCCAGC AGTTCAGTCC ATAGGAATAC CAGCTAACCT TGGACAAGCT 
GCTGAGGCTT CACTTCCCAC TGGAGATTCT CTATACCAGG GATTCCCACC TCGACTGCCA 
CCTCAATACC CAGGAGACTC AAATATTATT CCCTCATCCA CCGTGGCTTC TGTTTGCGTC 
CCTTCTACAG TTCTGTCCCC TCCCTTGCCC ACAGAAGCAT TGGCTCCACC AGGGTACCTT 
CCTACAGTTG TGCAGCCATA TGTCGAGCCA AGTGTTTTAG TTCCTGTGGG TAGTCTAGGA 
GGACAGGTTC AAGTACCCCA TTCAGCAGTC ACCTTAGCAC AGCAGGCCCC CTCTGCACCC 
TCCCAGCCAG CAGCTCTGGA GACACAAGCA GTTTCCCAGT CCAGTCCTCC AGAACCTGTG 
CCATCAGCAC AGTCACAGAC CACTACATTG GTGTCCTCTA TAGATAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGGAATGAG AATGTTCCTT CATCCAGTGG GAGACATGAA 
GGGAGAACTA CAAAACGCCA TTACCGAAAG TCTGTACGGA GTCGTTCTCG TCATGAGAAG 
ACTGCTCGCC CCAAATTGAG AATTCTTAAT GTGTCAAACA AAGGAGACAG AGTGGTGGAA 
TGCCAGTTAG AGACTCACAA CCGGAAAATG GTTACATTCA AATTTGACTT GGATGGTGAC 
AATCCTGAGG AGATAGCATC GATTATGGTG AACAATGATT TTATTCTAGC AACAGAGAGA 
GACTCCTTTG TGGATCAGGT GCGAGAAATC ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTTAGTG TGGAACCAGA AGGTGATCAA GGATCAGAAA GCTTACAAAC AAAGGATGAT 
TATGGCTTTT CAGGATCCCA GAAATTGGAG GGAGAGTTCA AGCAACCAAA TCCTGCATCT 
TCTATGCCTC AGAGGGCAGG TGTTCCCTCC AGTTCATTAA CTCAAGTTGT ACATTCAGCT 
GGACGGAGGT TCATAGTGAG TCCTGTACCA GAGAGTCGGT TACGAGAGTC AGTATTTTTC 
ACTGCTACAT CAAGTGAAAT ATCAGACACA ACTGCTGTTG CTCCATCCCA TGGCCCTGTA 
ATGAATCTGT CTCATTCTGC TTCATCCGTT AGTTTGCAAC AGGCATTCTC TGAACTTAGA 
CATGCTCAAA TGTCAGAAGG ACCCAGTACA GCTCCCCCAA ACTTCAGCCA TGCAGGACCA 
ACCTTTCCAA CTGTACCTGC CACTATGACT AGCATGGCTG TAGTCTTCCC TCCCACGGCA 
ACAGCTGTGC CTTCAGTATC TGTCCCTGCT ACAAGTAGCC CTTTTAATAG TGTTTCTGCA 
CCAGTCATTC AATCTGTGGT CACTATGCCT ACTGAAAAGG TAATTGCCGG AGTTGCCTCC 
AGTGCAGGTG TATTAACTTC AAGTTCTTTT CCTGTACCAC AGGTGTCTGG ACCACCAGTA 
ATGACCAGTG TGGTTTCCAG CATTACTATT CCTGCAGCCG TGTCATTGTC TACTGCAAGT 
GAATCAGTTC AGGCCCCACC AGTTGGGGGA GTTATGTCTA GTGCTGGCAC TTTTCCTCCT 
GCAGCGGGAT TGGTAGTTCC AGTTCCTGGA ATAGCCAGTA CAGTTCCCCC AGGTGCTATC 
ACACCAGCTA TGACTTCTCA GCCCACCCCA ATTGCAACAA ATATAACTGG GGCAGCCACC 
CTGACTTCCA CATCTGCCAC CACTCCGTTC CCAAGCATGG CTTCACAACC ACCTCTTCAA 
ATCAGTAGCA GCAGCTCTGC TCCCACTGTA GCAGAAACTG TGGTAGTCAG TGCACTCCAT 
CCCAAACACG ATGGCACACA TTTACCAGAC AAGCCACCCC TTAGCAGTGC AGCAGGGCTC 
TCCCTGCCCC TCTCAGTAGC TCCATCTTCC TCTGGAGCTG GGATCTCTGA TTCAGCTAGT 
CAGCCCAGTG TGCTGCATCC TTTACAGGTC CCATCCGCTG TGGCTTCTCC TCCAGTACTT 
TCACAGGCAG CAGGGACCGT CTCCACCCCG GTGTTACCTC AGGTACCTTT ACCTGGTATC 
CCGCCCCTAG CACAGCCTAT TACCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAACCTCAAC CAGCTTCACT TCCCAACCAG CCCCACACTC ATTGCCCTGA AGTAGATACT 
GATACACAAC CCAAAGCCCC TGGCATTGAT GATATAAAGA CTCTGGAAGA AAAACTGCGG 
TCTCTCTTCA GTGAACACAG TTCTGTTGGA GCCCAGCATG CCCCTGTCTC CCTGGAGACA 
TCATTGGTAG TGGAGACTAC AGTCATGCCA GGCATCCCAA CCACCGCTGT GGCACCAAGC 
AAGCTCATGA CTTCCACCAC GAGCACTTGC TTACCACCAA GCAGTTTGCC TCTAGGAACA 
ACTACTGGTT TGTCAGCTGT TACACCAGTG GTCACACCCG GGCAGGCTGC CACCCCAGTC 
AGCTATGTTC CAGCCCCCAC CAGCACTGCA GTAGGGGTGA AACCTGGAAC CCCTCCCTCA 
AAGCCACCTA TCGCCAGGGT ACCGGTACAA CCAGTAGGTA CTGAACTTCA ATCTGGTACC 
CCACCCAGCG AGCAGCTGCC GCCTTTTCCA GGACCTTCCC TAACACAGTC TCAGCAACCT 
CTAGAAGATC TTGATGCTCA GTTAAGAAGA GCCCTTAGTC CAGAGACTGT CCCAGTCACA 
TCTTCAGTTG CTCCCACATC AGTTGTGACT CCAACATCAG TTGCTGGAGC AGTGCCAGTG 
GTAGTGCCAG CTCAGAAAGC AGCTTCAAGC ATAGAAGATA ATACCATGAT GACCAACTCA 
GAGGCTAGCG TTCTTAAAAT GGGCCGGTTT CAGGTATCAG TTGCAGTAGA TGATTCCTTG 
AAGGAAAATA GAAGCAAGTC GGAAGAAATC AAGTCTGTAC ACTTTGAATC AAGTACTTCA 
GAGTCCTCAG TGCTGTCCAG TAGTAGCCCA GAGAATACAC TGGTGAAGCA GGAGCCTGGT 
GGAGTCACCA CAGCTCTATG TAGCCCCTCT TTGGATATGG TTGACGGTGT TCCTTCTCAA 
AAAACCTCTG CTACCCAGAC AAAGCCCGAA GCTGAGCAGC CTGCCACAGT GGTTGGCCGA 
TTCTCTGTGT CAAAAACTGA AGATAAGGTC ACCCCTGTGG TGGAGGGAGA GCCACCTGTA 
GTACGCCCTT TATTGGATTC TGAACAGGCC GTTCCTTCTG CTGTAGCACC AAAGAAAGAA 
ATGCCTGAAT CGCCAGAGCC AAGCCATCTT AATGGGCCAT CTTGTGACCT AGAAGCTGCC 
TTTCTCAGTA GGAATATGGA TGATGGTTCA GGTAGCCCCC ATTCTACTCA CCATTTGGGC 
TCAAAAGGCA GCCTTCCCAT TCAGAGCCTT AGCCAGAGCC TTAGCAATTC ATTCAACTCC 
TCTTACATGA GCAGTGACAA TGAGTCAGAC ATTGAAGATG AGGACTTGAA GTCAGAACTA 
CGTCGGCTAC GAGAAAAGCA CCTTAAAGAG ATCCAGGACC TACAGAGTCG CCAGAAACAT 
GAAATCGAGT CTTTATATAC CAAATTGGGC AAAGTCCCTC CTGCAGTCAT CATTCCCCCA 
GCTGCTCCAC TCTCAGGGAG AAGAAGACGA CCCACTAAAA GCAAAGGCAG CAAATCTAGC 
CGTAGTAGTT CTTTAGGAAA TAAGAGCCCC CAACTTTCAG GTAACCTGTC TGCTCAGAGT 
GCAGCTTCAG TCTTGCACCC CCAGCAGACG CTCCACCCTC CAGGCAGCAT CCCAGAGACT 
GGGCAGAATC AGCTGTTACA GCCCCTTAAA CCATCTCCCT CCAGTGAGAA CCTCTATTCA 
GCTTTTACCA GTGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCTCCAGG CCAAGGCTGT 
GCGAAGTTTA ACTGTGCATC TGAGCGGGTG ACATTCAAAC CTGGTGGCAG GAGGACCCGA 
TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC 
CAGCTCTGTA GGACCAGCAG TACGAATACA GTGGGAGGAG CAGTCAATAG CCAAGCCCCA 
CAAGCCCAGC CGCCCAGTAG AAAGGGCACA TTTACAGATG ATCTACACAA GTTGGTAGAC 
AACTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGGAGAG GAAGCAAGGG GCACATGAAC 
TATGAGGGTC CTGGAATGGC AAGGAAATTT TCAGCACCTG GACAGCTCTG CATCTCCATG 
ACCTCTAATC TGGGTGGTTC TGCCCCCATT TCTGCAGCAT CAGCTACCTC TTTAGGTCAC 
TTCACCAAGG CTATGTGCCC CCCACAGCAG TACAGCTTTC CATCTGCCCC TTTCAGCACT 
CAGTGGAGTG GGACAAGTGG CCCAGCACCG CCACCCCTCA GCCAGTTCCA GCCTGTAGGG 
ACTTCGTCCT TGCAGAGTTT CAACATCAGC AATTTGCAAA AATCCATCAG CAATCCTCCA 
GGTTCAAACC TGAGGACAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795402.1
CDS	513..6536
Translation	MAGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALETQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGCAKFNCASERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_031939542.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939542.1

ORF Insert Sequence:

ATGGCGGGAG TAAAGCCAGC CAGCTTTGAC AAAGTAGCAA TTCCTGAAGT AAAGGAAATT 
ATTGAAGGAT GCATACGCCA AAACAAAGAT GAAAGATATG CTATCAAAGA CCTTTTGAAC 
CATGCCTTCT TCCAGGAGGA AACAGGAGTA CGAGTAGAAC TAGCTGAAGA AGATGATGGT 
GAAAAGATAG CCATTAAGCT ATGGTTACGC ATTGAAGATA TTAAGAAATT GAAGGGGAAA 
TACAAAGACA ATGAAGCAAT TGAATTTTCC TTTGATTTGG AGAGAGATGT CCCAGAAGAT 
GTAGCTCAAG AAATGGTAGA ATCAGGGTAT GTTTGTGAAG GAGATCACAA GACCATGGCC 
AAAGCCATCA AAGACAGAGT GTCCCTTATA AAGAGAAAAC GAGAGCAGCG ACAGTTGGTA 
AGGGAAGAAC AGGAGAAAAG AAAGCAAGAA GAGATCAGTT TAAAGGAGCA AGTTGAACAG 
CCAACAACCA TTTCTCAGGC TGGGACCAAA TTGATTCCTT CTACCCCTGG CACAGTTACT 
GTCTCTACCA CATCAGCCTC AGTTTCCACC CAAGTGGAGC CAGAAGAACC AGAGGCAGAT 
CAACACCAAC AACTGCAGTA TCAACAACCT AGTCTCTCCG TGTTGTCTGA TGGAACAGTT 
GACAGTGGTC AGGGGTCTTC AGTCTTCACA GAATCTCGAG TAAGCAGCCA GCAGACTGTC 
TCATATGGGT CTCAACATGA ACAGGCACTT TCAACAGGCA CGATCCCAAG CTATACAGCT 
TCTGTTGTTC AAGCACAATC CCAGCCTCAT GGTGTATATC CTTCCTCCAC CGTGACACCG 
GCACAGAGCC AGAGTCATCT GGCTTCAAGT AACTTAACTG GGATTCCATC TTCCCAGACA 
GCACAGCTTG CTCAGCAGGG TATACAACAA GCAGCCCCTC CTCAGCAGAC AGGCCAGTAT 
CTTCTTCCCC AGTCAGCAGC ATCTGGTGTG CCTACAACTG CACAGCCTGT GACGCAGCCC 
CAGCTTCCAC AGGTTCTGCC AATGCCTCAA GTATCAGTTG GGAAGCAGCT TCCTGTTTCT 
CAGCCAGTAT CGACCATCCA AGGCGAATCT CAGATACCAG TTGCAGCACC TTCCCTTCCA 
CAGCCCTCAG TTAACCCATC TCTCTCGGGT GCTCACTTCC TTCCTGTGGG CCAGCCACTT 
CCAGCATCCT TAATTCCTCA GTTCTCAGTC TCTCAGATTC CCATGTCAAC TCCTCATGTC 
TCTGTGGCTC AGACAGGTTT CTCATCCCTG CCAAGCTCTA TAGCATCTGG CATTAACCAG 
CCTCTGCTCA CCTTGGCCTC ATCTGCTGCA GCGGCTGCTA TCCCTGTGGG ATCAACTATG 
GTCCCTAGTC AGCTTCCCAC CCTTCTGCAG CCTGTGACTC AGCTGCCAAG TCAGGCTCAC 
CCACAGCTCC TTCAGCCAGC AGTTCAGTCC ATAGGAATAC CAGCTAACCT TGGACAAGCT 
GCTGAGGCTT CACTTCCCAC TGGAGATTCT CTATACCAGG GATTCCCACC TCGACTGCCA 
CCTCAATACC CAGGAGACTC AAATATTATT CCCTCATCCA CCGTGGCTTC TGTTTGCGTC 
CCTTCTACAG TTCTGTCCCC TCCCTTGCCC ACAGAAGCAT TGGCTCCACC AGGGTACCTT 
CCTACAGTTG TGCAGCCATA TGTCGAGCCA AGTGTTTTAG TTCCTGTGGG TAGTCTAGGA 
GGACAGGTTC AAGTACCCCA TTCAGCAGTC ACCTTAGCAC AGCAGGCCCC CTCTGCACCC 
TCCCAGCCAG CAGCTCTGGA GACACAAGCA GTTTCCCAGT CCAGTCCTCC AGAACCTGTG 
CCATCAGCAC AGTCACAGAC CACTACATTG GTGTCCTCTA TAGATAGTGC ACATTCAGAT 
GTTGCTTCAG GTATGAGTGA TGGGAATGAG AATGTTCCTT CATCCAGTGG GAGACATGAA 
GGGAGAACTA CAAAACGCCA TTACCGAAAG TCTGTACGGA GTCGTTCTCG TCATGAGAAG 
ACTGCTCGCC CCAAATTGAG AATTCTTAAT GTGTCAAACA AAGGAGACAG AGTGGTGGAA 
TGCCAGTTAG AGACTCACAA CCGGAAAATG GTTACATTCA AATTTGACTT GGATGGTGAC 
AATCCTGAGG AGATAGCATC GATTATGGTG AACAATGATT TTATTCTAGC AACAGAGAGA 
GACTCCTTTG TGGATCAGGT GCGAGAAATC ATTGAAAAAG CTGATGAAAT GCTCAGTGAG 
GATGTTAGTG TGGAACCAGA AGGTGATCAA GGATCAGAAA GCTTACAAAC AAAGGATGAT 
TATGGCTTTT CAGGATCCCA GAAATTGGAG GGAGAGTTCA AGCAACCAAA TCCTGCATCT 
TCTATGCCTC AGAGGGCAGG TGTTCCCTCC AGTTCATTAA CTCAAGTTGT ACATTCAGCT 
GGACGGAGGT TCATAGTGAG TCCTGTACCA GAGAGTCGGT TACGAGAGTC AGTATTTTTC 
ACTGCTACAT CAAGTGAAAT ATCAGACACA ACTGCTGTTG CTCCATCCCA TGGCCCTGTA 
ATGAATCTGT CTCATTCTGC TTCATCCGTT AGTTTGCAAC AGGCATTCTC TGAACTTAGA 
CATGCTCAAA TGTCAGAAGG ACCCAGTACA GCTCCCCCAA ACTTCAGCCA TGCAGGACCA 
ACCTTTCCAA CTGTACCTGC CACTATGACT AGCATGGCTG TAGTCTTCCC TCCCACGGCA 
ACAGCTGTGC CTTCAGTATC TGTCCCTGCT ACAAGTAGCC CTTTTAATAG TGTTTCTGCA 
CCAGTCATTC AATCTGTGGT CACTATGCCT ACTGAAAAGG TAATTGCCGG AGTTGCCTCC 
AGTGCAGGTG TATTAACTTC AAGTTCTTTT CCTGTACCAC AGGTGTCTGG ACCACCAGTA 
ATGACCAGTG TGGTTTCCAG CATTACTATT CCTGCAGCCG TGTCATTGTC TACTGCAAGT 
GAATCAGTTC AGGCCCCACC AGTTGGGGGA GTTATGTCTA GTGCTGGCAC TTTTCCTCCT 
GCAGCGGGAT TGGTAGTTCC AGTTCCTGGA ATAGCCAGTA CAGTTCCCCC AGGTGCTATC 
ACACCAGCTA TGACTTCTCA GCCCACCCCA ATTGCAACAA ATATAACTGG GGCAGCCACC 
CTGACTTCCA CATCTGCCAC CACTCCGTTC CCAAGCATGG CTTCACAACC ACCTCTTCAA 
ATCAGTAGCA GCAGCTCTGC TCCCACTGTA GCAGAAACTG TGGTAGTCAG TGCACTCCAT 
CCCAAACACG ATGGCACACA TTTACCAGAC AAGCCACCCC TTAGCAGTGC AGCAGGGCTC 
TCCCTGCCCC TCTCAGTAGC TCCATCTTCC TCTGGAGCTG GGATCTCTGA TTCAGCTAGT 
CAGCCCAGTG TGCTGCATCC TTTACAGGTC CCATCCGCTG TGGCTTCTCC TCCAGTACTT 
TCACAGGCAG CAGGGACCGT CTCCACCCCG GTGTTACCTC AGGTACCTTT ACCTGGTATC 
CCGCCCCTAG CACAGCCTAT TACCAATGTG CCTGCTGTAC AGCAGACACT AATTCATAGT 
CAACCTCAAC CAGCTTCACT TCCCAACCAG CCCCACACTC ATTGCCCTGA AGTAGATACT 
GATACACAAC CCAAAGCCCC TGGCATTGAT GATATAAAGA CTCTGGAAGA AAAACTGCGG 
TCTCTCTTCA GTGAACACAG TTCTGTTGGA GCCCAGCATG CCCCTGTCTC CCTGGAGACA 
TCATTGGTAG TGGAGACTAC AGTCATGCCA GGCATCCCAA CCACCGCTGT GGCACCAAGC 
AAGCTCATGA CTTCCACCAC GAGCACTTGC TTACCACCAA GCAGTTTGCC TCTAGGAACA 
ACTACTGGTT TGTCAGCTGT TACACCAGTG GTCACACCCG GGCAGGCTGC CACCCCAGTC 
AGCTATGTTC CAGCCCCCAC CAGCACTGCA GTAGGGGTGA AACCTGGAAC CCCTCCCTCA 
AAGCCACCTA TCGCCAGGGT ACCGGTACAA CCAGTAGGTA CTGAACTTCA ATCTGGTACC 
CCACCCAGCG AGCAGCTGCC GCCTTTTCCA GGACCTTCCC TAACACAGTC TCAGCAACCT 
CTAGAAGATC TTGATGCTCA GTTAAGAAGA GCCCTTAGTC CAGAGACTGT CCCAGTCACA 
TCTTCAGTTG CTCCCACATC AGTTGTGACT CCAACATCAG TTGCTGGAGC AGTGCCAGTG 
GTAGTGCCAG CTCAGAAAGC AGCTTCAAGC ATAGAAGATA ATACCATGAT GACCAACTCA 
GAGGCTAGCG TTCTTAAAAT GGGCCGGTTT CAGGTATCAG TTGCAGTAGA TGATTCCTTG 
AAGGAAAATA GAAGCAAGTC GGAAGAAATC AAGTCTGTAC ACTTTGAATC AAGTACTTCA 
GAGTCCTCAG TGCTGTCCAG TAGTAGCCCA GAGAATACAC TGGTGAAGCA GGAGCCTGGT 
GGAGTCACCA CAGCTCTATG TAGCCCCTCT TTGGATATGG TTGACGGTGT TCCTTCTCAA 
AAAACCTCTG CTACCCAGAC AAAGCCCGAA GCTGAGCAGC CTGCCACAGT GGTTGGCCGA 
TTCTCTGTGT CAAAAACTGA AGATAAGGTC ACCCCTGTGG TGGAGGGAGA GCCACCTGTA 
GTACGCCCTT TATTGGATTC TGAACAGGCC GTTCCTTCTG CTGTAGCACC AAAGAAAGAA 
ATGCCTGAAT CGCCAGAGCC AAGCCATCTT AATGGGCCAT CTTGTGACCT AGAAGCTGCC 
TTTCTCAGTA GGAATATGGA TGATGGTTCA GGTAGCCCCC ATTCTACTCA CCATTTGGGC 
TCAAAAGGCA GCCTTCCCAT TCAGAGCCTT AGCCAGAGCC TTAGCAATTC ATTCAACTCC 
TCTTACATGA GCAGTGACAA TGAGTCAGAC ATTGAAGATG AGGACTTGAA GTCAGAACTA 
CGTCGGCTAC GAGAAAAGCA CCTTAAAGAG ATCCAGGACC TACAGAGTCG CCAGAAACAT 
GAAATCGAGT CTTTATATAC CAAATTGGGC AAAGTCCCTC CTGCAGTCAT CATTCCCCCA 
GCTGCTCCAC TCTCAGGGAG AAGAAGACGA CCCACTAAAA GCAAAGGCAG CAAATCTAGC 
CGTAGTAGTT CTTTAGGAAA TAAGAGCCCC CAACTTTCAG GTAACCTGTC TGCTCAGAGT 
GCAGCTTCAG TCTTGCACCC CCAGCAGACG CTCCACCCTC CAGGCAGCAT CCCAGAGACT 
GGGCAGAATC AGCTGTTACA GCCCCTTAAA CCATCTCCCT CCAGTGAGAA CCTCTATTCA 
GCTTTTACCA GTGATGGTGC CATTTCAGTA CCAAGCCTTT CTGCTCCAGG CCAAGGCTGT 
GCGAAGTTTA ACTGTGCATC TGAGCGGGTG ACATTCAAAC CTGGTGGCAG GAGGACCCGA 
TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG AAGATGGTGA AAAAAGTCTG TCCTTGCAAC 
CAGCTCTGTA GGACCAGCAG TACGAATACA GTGGGAGGAG CAGTCAATAG CCAAGCCCCA 
CAAGCCCAGC CGCCCAGTAG AAAGGGCACA TTTACAGATG ATCTACACAA GTTGGTAGAC 
AACTGGGCCC GAGATGCCAT GAATCTCTCA GGCAGGAGAG GAAGCAAGGG GCACATGAAC 
TATGAGGGTC CTGGAATGGC AAGGAAATTT TCAGCACCTG GACAGCTCTG CATCTCCATG 
ACCTCTAATC TGGGTGGTTC TGCCCCCATT TCTGCAGCAT CAGCTACCTC TTTAGGTCAC 
TTCACCAAGG CTATGTGCCC CCCACAGCAG TACAGCTTTC CATCTGCCCC TTTCAGCACT 
CAGTGGAGTG GGACAAGTGG CCCAGCACCG CCACCCCTCA GCCAGTTCCA GCCTGTAGGG 
ACTTCGTCCT TGCAGAGTTT CAACATCAGC AATTTGCAAA AATCCATCAG CAATCCTCCA 
GGTTCAAACC TGAGGACAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK1 ( XM_031939543.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031795403.1 Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

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CloneID	OSa237683
Clone ID Related Accession (Same CDS sequence)	XM_031939543.1
Accession Version	XM_031939543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	serine/threonine-protein kinase WNK1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045430.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG CAGAATGACA TACCATTGTG GGGGCCTTGT 
TGCCACTGGC TGTTCTGTTG GGAAGTGAGT ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGTACGAA TACAGTGGGA GGAGCAGTCA ATAGCCAAGC CCCACAAGCC 
CAGCCGCCCA GTAGAAAGGG CACATTTACA GATGATCTAC ACAAGTTGGT AGACAACTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGG AGAGGAAGCA AGGGGCACAT GAACTATGAG 
GGTCCTGGAA TGGCAAGGAA ATTTTCAGCA CCTGGACAGC TCTGCATCTC CATGACCTCT 
AATCTGGGTG GTTCTGCCCC CATTTCTGCA GCATCAGCTA CCTCTTTAGG TCACTTCACC 
AAGGCTATGT GCCCCCCACA GCAGTACAGC TTTCCATCTG CCCCTTTCAG CACTCAGTGG 
AGTGGGACAA GTGGCCCAGC ACCGCCACCC CTCAGCCAGT TCCAGCCTGT AGGGACTTCG 
TCCTTGCAGA GTTTCAACAT CAGCAATTTG CAAAAATCCA TCAGCAATCC TCCAGGTTCA 
AACCTGAGGA CAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031795403.1
CDS	1085..8362
Translation	MSGGSVEKQSGPAGPRFLSPPAVPPKNSSSSDTSVGEKLGAAGAEPGAGRTEEYRRRRHTMDKDSRGSAATTTTTEHRFFRRSVICDSNATALELPGLPLPLPQPGPVAAATAAPPPTLAPEPHREEPLTAPAASPAAPQPPAPPAPADHPNGGPASAAGRDPAVKHAPTPAASNHVGSKEEPAAAAAAAAAASGSAGAREQEERSQQQDDNEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKRKQEEISLKEQVEQPTTISQAGTKLIPSTPGTVTVSTTSASVSTQVEPEEPEADQHQQLQYQQPSLSVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQALSTGTIPSYTASVVQAQSQPHGVYPSSTVTPAQSQSHLASSNLTGIPSSQTAQLAQQGIQQAAPPQQTGQYLLPQSAASGVPTTAQPVTQPQLPQVLPMPQVSVGKQLPVSQPVSTIQGESQIPVAAPSLPQPSVNPSLSGAHFLPVGQPLPASLIPQFSVSQIPMSTPHVSVAQTGFSSLPSSIASGINQPLLTLASSAAAAAIPVGSTMVPSQLPTLLQPVTQLPSQAHPQLLQPAVQSIGIPANLGQAAEASLPTGDSLYQGFPPRLPPQYPGDSNIIPSSTVASVCVPSTVLSPPLPTEALAPPGYLPTVVQPYVEPSVLVPVGSLGGQVQVPHSAVTLAQQAPSAPSQPAALEQNDIPLWGPCCHWLFCWEVSTQAVSQSSPPEPVPSAQSQTTTLVSSIDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVNNDFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGSESLQTKDDYGFSGSQKLEGEFKQPNPASSMPQRAGVPSSSLTQVVHSAGRRFIVSPVPESRLRESVFFTATSSEISDTTAVAPSHGPVMNLSHSASSVSLQQAFSELRHAQMSEGPSTAPPNFSHAGPTFPTVPATMTSMAVVFPPTATAVPSVSVPATSSPFNSVSAPVIQSVVTMPTEKVIAGVASSAGVLTSSSFPVPQVSGPPVMTSVVSSITIPAAVSLSTASESVQAPPVGGVMSSAGTFPPAAGLVVPVPGIASTVPPGAITPAMTSQPTPIATNITGAATLTSTSATTPFPSMASQPPLQISSSSSAPTVAETVVVSALHPKHDGTHLPDKPPLSSAAGLSLPLSVAPSSSGAGISDSASQPSVLHPLQVPSAVASPPVLSQAAGTVSTPVLPQVPLPGIPPLAQPITNVPAVQQTLIHSQPQPASLPNQPHTHCPEVDTDTQPKAPGIDDIKTLEEKLRSLFSEHSSVGAQHAPVSLETSLVVETTVMPGIPTTAVAPSKLMTSTTSTCLPPSSLPLGTTTGLSAVTPVVTPGQAATPVSYVPAPTSTAVGVKPGTPPSKPPIARVPVQPVGTELQSGTPPSEQLPPFPGPSLTQSQQPLEDLDAQLRRALSPETVPVTSSVAPTSVVTPTSVAGAVPVVVPAQKAASSIEDNTMMTNSEASVLKMGRFQVSVAVDDSLKENRSKSEEIKSVHFESSTSESSVLSSSSPENTLVKQEPGGVTTALCSPSLDMVDGVPSQKTSATQTKPEAEQPATVVGRFSVSKTEDKVTPVVEGEPPVVRPLLDSEQAVPSAVAPKKEMPESPEPSHLNGPSCDLEAAFLSRNMDDGSGSPHSTHHLGSKGSLPIQSLSQSLSNSFNSSYMSSDNESDIEDEDLKSELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSAQSAASVLHPQQTLHPPGSIPETGQNQLLQPLKPSPSSENLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGAVNSQAPQAQPPSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKAMCPPQQYSFPSAPFSTQWSGTSGPAPPPLSQFQPVGTSSLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_031939543.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii WNK lysine deficient protein kinase 1 (WNK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031939543.1

ORF Insert Sequence:

ATGTCTGGTG GCTCCGTCGA GAAGCAGAGC GGACCTGCGG GGCCCCGCTT CCTCTCGCCC 
CCGGCCGTGC CCCCGAAAAA TAGCTCGAGC TCGGATACTT CAGTCGGGGA GAAGCTGGGG 
GCGGCCGGGG CTGAGCCCGG CGCCGGCAGG ACCGAGGAGT ACCGGCGGCG CCGCCACACC 
ATGGACAAGG ACAGCCGAGG GTCGGCCGCG ACCACCACCA CCACGGAGCA CCGCTTCTTC 
CGGCGGAGCG TGATCTGCGA CTCTAACGCC ACGGCCTTGG AGCTGCCCGG CCTTCCCCTG 
CCCTTGCCCC AGCCCGGGCC CGTCGCAGCC GCCACCGCCG CCCCCCCGCC GACTCTCGCC 
CCCGAGCCCC ACCGGGAGGA GCCCTTGACT GCCCCGGCCG CGTCCCCGGC AGCCCCCCAG 
CCTCCGGCCC CCCCGGCTCC CGCGGACCAC CCTAATGGCG GCCCTGCCTC CGCCGCCGGC 
CGCGACCCCG CCGTCAAGCA CGCCCCGACC CCCGCCGCCT CCAACCATGT GGGGAGCAAA 
GAGGAGCCCG CGGCGGCGGC GGCGGCGGCG GCGGCGGCGA GCGGCAGCGC CGGCGCCCGG 
GAACAGGAGG AGCGCAGCCA GCAGCAGGAC GACAACGAGG AGCTGGAGAC CAAGGCTGTG 
GGCATGTCCA ACGACGGCCG CTTTCTCAAG TTCGACATCG AGATCGGCCG CGGCTCCTTC 
AAGACCGTCT ACAAAGGCCT GGATACGGAA ACCACCGTGG AAGTGGCGTG GTGCGAACTG 
CAGGATAGGA AGTTGACAAA GTCAGAACGG CAAAGATTTA AAGAAGAGGC TGAAATGTTG 
AAGGGCCTTC AACATCCAAA TATTGTTCGT TTCTATGATT CTTGGGAGTC TACAGTAAAA 
GGAAAGAAAT GTATTGTTCT GGTGACTGAA CTTATGACAT CTGGAACCCT TAAAACGTAC 
CTTAAAAGGT TTAAAGTGAT GAAGATCAAA GTTTTAAGAA GCTGGTGCCG TCAGATTTTG 
AAAGGGCTTC AGTTTCTTCA TACTCGAACA CCACCTATTA TTCATCGGGA TCTTAAATGT 
GACAATATCT TTATCACTGG CCCAACAGGA TCAGTCAAGA TTGGAGACCT GGGTCTGGCA 
ACTCTGAAGC GGGCTTCTTT TGCCAAGAGT GTAATAGGTA CCCCAGAGTT CATGGCCCCT 
GAGATGTACG AGGAAAAATA TGATGAGTCC GTTGACGTGT ATGCTTTTGG AATGTGCATG 
CTTGAGATGG CCACATCCGA ATACCCTTAT TCTGAGTGCC AAAATGCTGC GCAGATCTAC 
CGTCGAGTGA CCAGTGGAGT AAAGCCAGCC AGCTTTGACA AAGTAGCAAT TCCTGAAGTA 
AAGGAAATTA TTGAAGGATG CATACGCCAA AACAAAGATG AAAGATATGC TATCAAAGAC 
CTTTTGAACC ATGCCTTCTT CCAGGAGGAA ACAGGAGTAC GAGTAGAACT AGCTGAAGAA 
GATGATGGTG AAAAGATAGC CATTAAGCTA TGGTTACGCA TTGAAGATAT TAAGAAATTG 
AAGGGGAAAT ACAAAGACAA TGAAGCAATT GAATTTTCCT TTGATTTGGA GAGAGATGTC 
CCAGAAGATG TAGCTCAAGA AATGGTAGAA TCAGGGTATG TTTGTGAAGG AGATCACAAG 
ACCATGGCCA AAGCCATCAA AGACAGAGTG TCCCTTATAA AGAGAAAACG AGAGCAGCGA 
CAGTTGGTAA GGGAAGAACA GGAGAAAAGA AAGCAAGAAG AGATCAGTTT AAAGGAGCAA 
GTTGAACAGC CAACAACCAT TTCTCAGGCT GGGACCAAAT TGATTCCTTC TACCCCTGGC 
ACAGTTACTG TCTCTACCAC ATCAGCCTCA GTTTCCACCC AAGTGGAGCC AGAAGAACCA 
GAGGCAGATC AACACCAACA ACTGCAGTAT CAACAACCTA GTCTCTCCGT GTTGTCTGAT 
GGAACAGTTG ACAGTGGTCA GGGGTCTTCA GTCTTCACAG AATCTCGAGT AAGCAGCCAG 
CAGACTGTCT CATATGGGTC TCAACATGAA CAGGCACTTT CAACAGGCAC GATCCCAAGC 
TATACAGCTT CTGTTGTTCA AGCACAATCC CAGCCTCATG GTGTATATCC TTCCTCCACC 
GTGACACCGG CACAGAGCCA GAGTCATCTG GCTTCAAGTA ACTTAACTGG GATTCCATCT 
TCCCAGACAG CACAGCTTGC TCAGCAGGGT ATACAACAAG CAGCCCCTCC TCAGCAGACA 
GGCCAGTATC TTCTTCCCCA GTCAGCAGCA TCTGGTGTGC CTACAACTGC ACAGCCTGTG 
ACGCAGCCCC AGCTTCCACA GGTTCTGCCA ATGCCTCAAG TATCAGTTGG GAAGCAGCTT 
CCTGTTTCTC AGCCAGTATC GACCATCCAA GGCGAATCTC AGATACCAGT TGCAGCACCT 
TCCCTTCCAC AGCCCTCAGT TAACCCATCT CTCTCGGGTG CTCACTTCCT TCCTGTGGGC 
CAGCCACTTC CAGCATCCTT AATTCCTCAG TTCTCAGTCT CTCAGATTCC CATGTCAACT 
CCTCATGTCT CTGTGGCTCA GACAGGTTTC TCATCCCTGC CAAGCTCTAT AGCATCTGGC 
ATTAACCAGC CTCTGCTCAC CTTGGCCTCA TCTGCTGCAG CGGCTGCTAT CCCTGTGGGA 
TCAACTATGG TCCCTAGTCA GCTTCCCACC CTTCTGCAGC CTGTGACTCA GCTGCCAAGT 
CAGGCTCACC CACAGCTCCT TCAGCCAGCA GTTCAGTCCA TAGGAATACC AGCTAACCTT 
GGACAAGCTG CTGAGGCTTC ACTTCCCACT GGAGATTCTC TATACCAGGG ATTCCCACCT 
CGACTGCCAC CTCAATACCC AGGAGACTCA AATATTATTC CCTCATCCAC CGTGGCTTCT 
GTTTGCGTCC CTTCTACAGT TCTGTCCCCT CCCTTGCCCA CAGAAGCATT GGCTCCACCA 
GGGTACCTTC CTACAGTTGT GCAGCCATAT GTCGAGCCAA GTGTTTTAGT TCCTGTGGGT 
AGTCTAGGAG GACAGGTTCA AGTACCCCAT TCAGCAGTCA CCTTAGCACA GCAGGCCCCC 
TCTGCACCCT CCCAGCCAGC AGCTCTGGAG CAGAATGACA TACCATTGTG GGGGCCTTGT 
TGCCACTGGC TGTTCTGTTG GGAAGTGAGT ACACAAGCAG TTTCCCAGTC CAGTCCTCCA 
GAACCTGTGC CATCAGCACA GTCACAGACC ACTACATTGG TGTCCTCTAT AGATAGTGCA 
CATTCAGATG TTGCTTCAGG TATGAGTGAT GGGAATGAGA ATGTTCCTTC ATCCAGTGGG 
AGACATGAAG GGAGAACTAC AAAACGCCAT TACCGAAAGT CTGTACGGAG TCGTTCTCGT 
CATGAGAAGA CTGCTCGCCC CAAATTGAGA ATTCTTAATG TGTCAAACAA AGGAGACAGA 
GTGGTGGAAT GCCAGTTAGA GACTCACAAC CGGAAAATGG TTACATTCAA ATTTGACTTG 
GATGGTGACA ATCCTGAGGA GATAGCATCG ATTATGGTGA ACAATGATTT TATTCTAGCA 
ACAGAGAGAG ACTCCTTTGT GGATCAGGTG CGAGAAATCA TTGAAAAAGC TGATGAAATG 
CTCAGTGAGG ATGTTAGTGT GGAACCAGAA GGTGATCAAG GATCAGAAAG CTTACAAACA 
AAGGATGATT ATGGCTTTTC AGGATCCCAG AAATTGGAGG GAGAGTTCAA GCAACCAAAT 
CCTGCATCTT CTATGCCTCA GAGGGCAGGT GTTCCCTCCA GTTCATTAAC TCAAGTTGTA 
CATTCAGCTG GACGGAGGTT CATAGTGAGT CCTGTACCAG AGAGTCGGTT ACGAGAGTCA 
GTATTTTTCA CTGCTACATC AAGTGAAATA TCAGACACAA CTGCTGTTGC TCCATCCCAT 
GGCCCTGTAA TGAATCTGTC TCATTCTGCT TCATCCGTTA GTTTGCAACA GGCATTCTCT 
GAACTTAGAC ATGCTCAAAT GTCAGAAGGA CCCAGTACAG CTCCCCCAAA CTTCAGCCAT 
GCAGGACCAA CCTTTCCAAC TGTACCTGCC ACTATGACTA GCATGGCTGT AGTCTTCCCT 
CCCACGGCAA CAGCTGTGCC TTCAGTATCT GTCCCTGCTA CAAGTAGCCC TTTTAATAGT 
GTTTCTGCAC CAGTCATTCA ATCTGTGGTC ACTATGCCTA CTGAAAAGGT AATTGCCGGA 
GTTGCCTCCA GTGCAGGTGT ATTAACTTCA AGTTCTTTTC CTGTACCACA GGTGTCTGGA 
CCACCAGTAA TGACCAGTGT GGTTTCCAGC ATTACTATTC CTGCAGCCGT GTCATTGTCT 
ACTGCAAGTG AATCAGTTCA GGCCCCACCA GTTGGGGGAG TTATGTCTAG TGCTGGCACT 
TTTCCTCCTG CAGCGGGATT GGTAGTTCCA GTTCCTGGAA TAGCCAGTAC AGTTCCCCCA 
GGTGCTATCA CACCAGCTAT GACTTCTCAG CCCACCCCAA TTGCAACAAA TATAACTGGG 
GCAGCCACCC TGACTTCCAC ATCTGCCACC ACTCCGTTCC CAAGCATGGC TTCACAACCA 
CCTCTTCAAA TCAGTAGCAG CAGCTCTGCT CCCACTGTAG CAGAAACTGT GGTAGTCAGT 
GCACTCCATC CCAAACACGA TGGCACACAT TTACCAGACA AGCCACCCCT TAGCAGTGCA 
GCAGGGCTCT CCCTGCCCCT CTCAGTAGCT CCATCTTCCT CTGGAGCTGG GATCTCTGAT 
TCAGCTAGTC AGCCCAGTGT GCTGCATCCT TTACAGGTCC CATCCGCTGT GGCTTCTCCT 
CCAGTACTTT CACAGGCAGC AGGGACCGTC TCCACCCCGG TGTTACCTCA GGTACCTTTA 
CCTGGTATCC CGCCCCTAGC ACAGCCTATT ACCAATGTGC CTGCTGTACA GCAGACACTA 
ATTCATAGTC AACCTCAACC AGCTTCACTT CCCAACCAGC CCCACACTCA TTGCCCTGAA 
GTAGATACTG ATACACAACC CAAAGCCCCT GGCATTGATG ATATAAAGAC TCTGGAAGAA 
AAACTGCGGT CTCTCTTCAG TGAACACAGT TCTGTTGGAG CCCAGCATGC CCCTGTCTCC 
CTGGAGACAT CATTGGTAGT GGAGACTACA GTCATGCCAG GCATCCCAAC CACCGCTGTG 
GCACCAAGCA AGCTCATGAC TTCCACCACG AGCACTTGCT TACCACCAAG CAGTTTGCCT 
CTAGGAACAA CTACTGGTTT GTCAGCTGTT ACACCAGTGG TCACACCCGG GCAGGCTGCC 
ACCCCAGTCA GCTATGTTCC AGCCCCCACC AGCACTGCAG TAGGGGTGAA ACCTGGAACC 
CCTCCCTCAA AGCCACCTAT CGCCAGGGTA CCGGTACAAC CAGTAGGTAC TGAACTTCAA 
TCTGGTACCC CACCCAGCGA GCAGCTGCCG CCTTTTCCAG GACCTTCCCT AACACAGTCT 
CAGCAACCTC TAGAAGATCT TGATGCTCAG TTAAGAAGAG CCCTTAGTCC AGAGACTGTC 
CCAGTCACAT CTTCAGTTGC TCCCACATCA GTTGTGACTC CAACATCAGT TGCTGGAGCA 
GTGCCAGTGG TAGTGCCAGC TCAGAAAGCA GCTTCAAGCA TAGAAGATAA TACCATGATG 
ACCAACTCAG AGGCTAGCGT TCTTAAAATG GGCCGGTTTC AGGTATCAGT TGCAGTAGAT 
GATTCCTTGA AGGAAAATAG AAGCAAGTCG GAAGAAATCA AGTCTGTACA CTTTGAATCA 
AGTACTTCAG AGTCCTCAGT GCTGTCCAGT AGTAGCCCAG AGAATACACT GGTGAAGCAG 
GAGCCTGGTG GAGTCACCAC AGCTCTATGT AGCCCCTCTT TGGATATGGT TGACGGTGTT 
CCTTCTCAAA AAACCTCTGC TACCCAGACA AAGCCCGAAG CTGAGCAGCC TGCCACAGTG 
GTTGGCCGAT TCTCTGTGTC AAAAACTGAA GATAAGGTCA CCCCTGTGGT GGAGGGAGAG 
CCACCTGTAG TACGCCCTTT ATTGGATTCT GAACAGGCCG TTCCTTCTGC TGTAGCACCA 
AAGAAAGAAA TGCCTGAATC GCCAGAGCCA AGCCATCTTA ATGGGCCATC TTGTGACCTA 
GAAGCTGCCT TTCTCAGTAG GAATATGGAT GATGGTTCAG GTAGCCCCCA TTCTACTCAC 
CATTTGGGCT CAAAAGGCAG CCTTCCCATT CAGAGCCTTA GCCAGAGCCT TAGCAATTCA 
TTCAACTCCT CTTACATGAG CAGTGACAAT GAGTCAGACA TTGAAGATGA GGACTTGAAG 
TCAGAACTAC GTCGGCTACG AGAAAAGCAC CTTAAAGAGA TCCAGGACCT ACAGAGTCGC 
CAGAAACATG AAATCGAGTC TTTATATACC AAATTGGGCA AAGTCCCTCC TGCAGTCATC 
ATTCCCCCAG CTGCTCCACT CTCAGGGAGA AGAAGACGAC CCACTAAAAG CAAAGGCAGC 
AAATCTAGCC GTAGTAGTTC TTTAGGAAAT AAGAGCCCCC AACTTTCAGG TAACCTGTCT 
GCTCAGAGTG CAGCTTCAGT CTTGCACCCC CAGCAGACGC TCCACCCTCC AGGCAGCATC 
CCAGAGACTG GGCAGAATCA GCTGTTACAG CCCCTTAAAC CATCTCCCTC CAGTGAGAAC 
CTCTATTCAG CTTTTACCAG TGATGGTGCC ATTTCAGTAC CAAGCCTTTC TGCTCCAGGC 
CAAGGGACCA GCAGTACGAA TACAGTGGGA GGAGCAGTCA ATAGCCAAGC CCCACAAGCC 
CAGCCGCCCA GTAGAAAGGG CACATTTACA GATGATCTAC ACAAGTTGGT AGACAACTGG 
GCCCGAGATG CCATGAATCT CTCAGGCAGG AGAGGAAGCA AGGGGCACAT GAACTATGAG 
GGTCCTGGAA TGGCAAGGAA ATTTTCAGCA CCTGGACAGC TCTGCATCTC CATGACCTCT 
AATCTGGGTG GTTCTGCCCC CATTTCTGCA GCATCAGCTA CCTCTTTAGG TCACTTCACC 
AAGGCTATGT GCCCCCCACA GCAGTACAGC TTTCCATCTG CCCCTTTCAG CACTCAGTGG 
AGTGGGACAA GTGGCCCAGC ACCGCCACCC CTCAGCCAGT TCCAGCCTGT AGGGACTTCG 
TCCTTGCAGA GTTTCAACAT CAGCAATTTG CAAAAATCCA TCAGCAATCC TCCAGGTTCA 
AACCTGAGGA CAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	WNK1
Entrez Gene ID	100925454
Full Name	WNK lysine deficient protein kinase 1
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

WNK1 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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