smchd1 cDNA ORF clone, Salmo salar(Atlantic salmon)

Gene
Clones
gRNAs

Summary

Gene Symbol	smchd1
Entrez Gene ID	106590141
Full Name	structural maintenance of chromosomes flexible hinge domain containing 1
Gene Type	protein-coding
Organism	Salmo salar(Atlantic salmon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014180770.1	XP_014036245.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2
XM_014180769.1	XP_014036244.1	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following smchd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smchd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAt73892	XM_014180769.1 Latest version!	Salmo salar structural maintenance of chromosomes flexible hinge domain containing 1 (smchd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OAt73893	XM_014180770.1 Latest version!	Salmo salar structural maintenance of chromosomes flexible hinge domain containing 1 (smchd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smchd1 ( XM_014180769.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014036244.1 Salmo salar structural maintenance of chromosomes flexible hinge domain containing 1 (smchd1), transcript variant X1, mRNA.

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CloneID	OAt73892
Clone ID Related Accession (Same CDS sequence)	XM_014180769.1
Accession Version	XM_014180769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012341707.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGT CGGAAGGTGT GACAACGTCT GATGTTGCTA ATAGCAATGG ATCACACATT 
TTACAGAGTA AAAATGGAGT TAACGGGTCA GATAAACCAC ATAAAAGTGT GGTAGTATTC 
GACTGTCGCC CGGAAAGAGT CGACGCAATG GAGAGAGTTC TGGACATAAA TGGCTTGGAC 
TTCAACGACT TCCTTCAAGT TGTATCGAAG GAATTTGCCA TTCCTTCCAA CGAAACGTTT 
GTCTTGACAA CAACGGACAG AAAAGGAATA GATTTTGACA GATATGAGGA ACTTCAACAA 
GGAAACACTC TCCACCTGTT GCAGACCGAG GACCAGATCC TGCCCGTGGC AACTAAGGAA 
CACATTGTGT TTCTCCCTCA CTATGACACA CTGGTCCAGA GTGGCATGTA TGAGTACTAC 
GCTAGTGAGG GCCAGAATTC TTTACCGTAT GCCTTTGCAG AACTTATCGA CAATGCACTG 
TCAGCTACAT CGCACAACAC AGGAGTGAGG ACCATAGACA TCCGATTGCT GTTTGATGAG 
TCCTTGGGGA AACATGCAGT GGTTATCATG GACAATGGCT GTGGAATGAC CTCCAAGCAG 
CTTAATAACT GGGCTGTGTA CAGGCTCTCC AAATTCAGCA GGGAAAGCAG CACGTTTTCA 
AGTGATCACG CAGGGTACGT TCGCCCTGAT CCAGTCGCTC GCAGTCTCAA CAGTGATATA 
TCATATTTTG GAGTAGGAGG GAAACAAGCA GTGTTCTACA TTGGACAGTC AACCAGGATG 
ATCACCAAAC CAGTGGGCTC CCCAGATGTC CATGAACTCA TCATTTCAAA AGAAGAATTT 
GAGAGGAAGG AGCGGGACAA GGAAGATATT TACAGTGGTT TCATCAGAAA CCGGAAGATG 
GGTGACTCGT CCCACGTGAG CGAGGACGAG GAGCGCTTCC TCCACGGGCT GATCGCTGAG 
GAGGTGGGAA AGGACAGCTT CACGGTCGTG GTGGTGACGG GAGTCCAGCC CGAACACATC 
ACCTTCCTCA AGCAGGACTT CCACCTGTGG ACCAGAGAAC TGGCCCACTT GTATCACTAT 
TACATTCACG GCCTAAATGG GAATGACATG AAATCCTCCT ACCGAAACTC TGATTGTGTT 
TCCAACATTG ACATTCAGAT CTCTCTGTTG GAGAAGTTCC CCAAGATCCC TCGCATGGTG 
AACCTCAGAG AGATCGACAA CGACATGCAG ACGCTCTACA TCAACACGTC GGCTGACACA 
TTCGAGTTCA AGGCCTGCAC AGACGTTGAC GGAATAGTGG AGGGGATCCT GAGGTACCAC 
CCCTTCCTCT ATGACAGGGA GACCTACCCC GAAGACCCCT ATGCCATACC AGCTACGGAA 
GAGGATGATG ACAGTATCGT CCTGAACCAG GCCAGGGGGA AGAGGCCCAT ATTTGAGTGT 
TTTTGGAATG GACGTCTCAT CCCATACACC ACTGTATCCG AGTTCGACTG GTGCGCATGG 
CCCAAGAAGA CGGGGACAGT GCCTGCAGAG TGTTACAGCA GGTTCTCAGG GGTGCTGTTC 
ACCAATGACA GGTTCCAGGT CAGCACCAAC AAGCTCACTT TCATGGACCT GGAGCTGCAG 
CTAAAGAATA AGGAGACCTT ATTCACCCGC ATCGTCAATG GCCAGGAACA AAGGGTTAAA 
ATCCAGAAGG AGTTTCACCA GTGGCTGAAG ACCTGCCACG AGAAGTGGGA CAAGCAAGTG 
AAGTTCAGAG GCTTCAAGAG AACCATCACA CGAACAGACG TGACCACAAA GAAGATGCAA 
TCTCCTTGGG CTACCTTCTC CTCCATCGAG TGGGACGGTA GAACTTACAA AGCTGGTCAA 
TATGTGAAGT CTCAGAAAAC ACAGCCCATC TTCTTTGGCT CCGTTATTCA GTTCCTACTG 
TACGGCGATC ACGAGGGAGA TGTCTACGCC ACAGGGGGGC AGGTGCAGGT GGCCATGGAA 
CCAAAGGCGC TGTACGATGA CATCAAGACA ATACCCATCT CCAAAATCGA CAGGGTGATT 
ACCAATGCAG CCATCAAGAA GAGCATTGAT GACGAACTAG CCAAACTCCC AGACAAGCTA 
AAAGTGACCT GGCCAGAGGG CAACCCCTGG ACCCAGAACG ATGTCCGGCC AGCAGGCACT 
CCACTAGGAC CTCTCCATGT GGAAATAATC AACAAGAAAG GAGAGTCAAT ATCCAGGATA 
CCATCAGCCA TGCAAAACGC AACACGGAAA GCACTTTCAA TCGAACTAAA AGTTGTTTGG 
CATTCACCTA ATGGTGATGT GGAGACCAAC TCTCACATTG CAGCACATTC GGCCAAGTGG 
GCGTTCTGGT TCAGAACCAT GGAGAACCTG AACAAGCTGG GGAAGTACAC TCTGCACCTG 
AACACAATAC TCAATGTCAA TGAGAGCAAC GCTACTGTCT GGGCTGGGAA TCGGCTTCCC 
AACCACACAC TGGACTTCTC CATTATTGAG GGCAGTGCAG AGAGGTTTGT GGTGGGTACA 
GTGAACACCC TGCTCCATGT GGGGGTGCCT TTCCACATCC CACTGGAGCT GCTGGATGAA 
TACGGTCACC CTGCTCGCCC CCCTCCCAAC CTCAAGCCCC TCCTGGAAAG CAGTGCCCTG 
GAAGTGAGTT ACGAGGGGGT GGCCGCTAGG GGGACCATTT TGACCATCCG AGGCGTCAGA 
GCCAGGGGGG CGGTGCAGAA CTACCAGAGC AAGACGCACG ACTTGAAAGT GGTACTGCCG 
GGCCTCAAAC AGGACTCGCA GACTCTGAAG ATCAGCCTTC TCCCTGGTAA CCCAGATTCA 
CTCAAAGTGA AACAAGAGGA TCCAATCACT GTGGAGAATG GAACTTCGGT TAGCTTTGAT 
GTGGAGGTCC ATGACGAAGC AGGAAATGTC ACAGCTCACC CCAAACTGAT CATCAGCTGC 
CAGTTGCAGG GGATTCCTGG TTCATCCTCC ATGGTGGCTG TAGACTGCAG TAACACAGGA 
GCCGGGACCC TGGTGCTAAA ACCAATCACC TTCAAAAACA TCGTGGGAGA CCAGACGGTT 
AAAGCAACAT TTGATATTCC CAGTCAGAAG AATGTAGCTG CAGTCCAGAG GGATCTGAGG 
GTTGTCCCCA GCACCCGTGT GTCACGTCTC GAGGTGTATA GTCAGGAGGA CGAGGACAGC 
CTGGTGGACT CCATGGTGCT TCGGGACAAG GAAAAGATAG ACTGGACGGC AGGGGACCTC 
CTGGAGAACC TCCATTATAG ACTGTATGAC GAGAGAGGCG GGGAGGTCCC TCTGTCTGAG 
GAGATGGCCC GGCGGATCAA GGTGAACTGG ACGGCTGACG TGAGTGTGGA GGAGCTGGCC 
CAGGGGAAGC TGCCCAACGT GCCGGTCCCG TCGCTGGTGA AGGAGGAGCA CTTCTACCAG 
GTCTCCTACA TGGAGGAGCT AGTGTCTGTC GACACCTCCT TCACTATAGT GCCCCGCCCC 
GATGAGCCGA AACACATGAA GGCCACCCTG AGCGAGTCGA CCGTGAGGAT GGGAGAGGTC 
CTATCAGGAA AGATCTTCTT ACAGCTTACA GACCAGTATG GTAATAAAAC TCAGATGCTG 
ACCGCGACCT GTGTGGACTC TCTCATCGTG GATGCCGAGG GTCTCGACAA AGCATCCCTG 
GTCTTTACCT GGCAGGAGAG CACCCACTCC ATGTTAGTGA CGGGGGTGCG GTTCAACCCT 
GGGTTCCCAG GCATCAGAGA GCTGAACTTC ACCTGGAGGG ACTTTACGGA GCATGTGACC 
ATTAAATTGA CGGCCGGGGT CCCGACACAG CTCAAACTAG TGGACGGACC ACAGGAGCCC 
TTGCAGGTAC TGAATGAGCA GGGCATCCAG ACGCCCTTCC TCCTCCAGCT GTTTGACGAG 
TGGGGGAACC CCTCTCCTGA CCAGAGGGTT GTGGTGACAC TGAAGACTTC CACTCCAGCT 
CTGAAGGTGA AGTCATCCGT TAATTCACAA CCGATAGACA AAGACGGAAA AGCCAGTTTC 
GTAGTGGCCC ATGTGAGCGG TCCAAAGTCT CCCAGGGGGG AGTATGCGCT GGAGTTCAGA 
GGGTCCTTCA ACAAGAAGCC CATCCACTGT CTATCCGTAA AGCTTACCAT TATCCCCGAT 
CCTAACAAAC CTGTCAAACT GGTGGTGGAG TACAACACAA ACTCAGTGTT TCCTGCTGGA 
GGCACCCTCA CAGTGTTTTC AGTGTCTGTT GTGTCGGAGG AGGGCAGCAC CATCAAGAAC 
CTCAATCCTG CCAACATGTC CATGCTGTTG TGGAAGGGAG AGCCGTCAGG GACGTCACGG 
CCCCCCTCAG GGGCTGCCCA ACTGAAATGC AGCAAACCCA TGGAAGACGA AAAGGCGGAC 
CGTTTTCACT TCAGAGACAA AGTCATCCCA GACCATGTCG GGAAGTACAC CATCCAGTTT 
GTGCTGTGTG TTGACAAGAC TAAAGGGCTG TGGAGTCACC AGTATGTCAT AAATGTTGTA 
CCCAACGATC CTGTCAAATT GGCCCCAGAC CTGCAGCCAC CAACCCCAGT GGTGGTTAAC 
AACAACGTCC TTGACAGTCG GATTCTGGTG GAGGACATGT CTCTGAAATT AATGGATATT 
TATAATAACT CAGCGGGGCT GGAGCTGAGT GGGAAGGTGG TGGTGACCAT TAAGAGCGCC 
AAAGGCTCCA GTGACAAAAA CCTGCCTCTC TTTGAGGGCA AAGCCAAGAG CCTGCAGTTT 
AGCCTGGCTA ATGGAGAGGC CCAAATCACT AATCTGTCCA TCATGGAGAA CAGTCCTGGT 
CAGGATGGGA ATGAGTATGT CTTGTTCTTC AGACCTTCCG TCCCAGGGTA TGGCCCCAAA 
AATCCCCTGG CAGCGTTTGA GCTTCCCTTC CGCTTCTACA ACGATGTGGA GAACAGGAAG 
CAGATGTCTG AACTGACCAA GAAGAAGGAC CAGCTCAAAC AGACCGTTGA TATCTACAGG 
AGTCTGTTTG ACACCAACAG GCAACTCATC ACTGAGCTCA GTAATCAGGT GCGCAACGCC 
ACCAACAAGG AGAGTCACTT GAAATCCGAG CTCATGAAGA ATGGCATGGC TGTAGCACAA 
CTGTCATCTA TCCCAGCGAT CGACGAAGTC ATCGGTCAGA AGACGGCTGA TGTGGAGAAG 
ATGAAACTCC AGACGCGGAG AGTCTGCTCC ATGCCCGATC CCTTCAGGGG CAACCCTGAC 
GTCCTGGGAA AAATTGGCCA CCTGGCGCTG GTGGAGGACA ACGACGTGGC CACGGTCATC 
TCCTGGCACC TCTTGGGGGA CATTGACTGT GTGGTCACCA TGACAACGGT GGCGGCACGG 
AGGATCTATG ACGACACCCA GGGGAAGCAG CAGGTCATGC CCCTGGACAC GGTGTTCTGG 
AAGAACAACA GCAGCAGACC CTTGCCCCAC AAAAGGAGGG ACGGTCAGGA CAGCTTTCGT 
CCCATTGGGA ACCCTGTGTT TGCCCGAGAC CTCCTCATTT TCCCAGAGAA TGCAGAGAAC 
TGCCAGATAG TGTTCGGAAA CCTCCTTGGA GACACCATTT TGACGGATGA CCTGGACTCT 
GCTAACCACT ACAGGAGGGG GGTGGTCCAG AGCAAGATGC CATGCCCCAC CCTCCTGACC 
CGGCAGGGCG ACAGGATCCG CAGCAACGGC AAGTTTGGGG GCTTGCAGAA CAAAGCCCCT 
CCAATAGAGA GACTGCGTGG CCAGGTGTTC GGCGCACCCC TCCCCAAGGA CTACCACACC 
TTTATGGGCC AGATTGACCT ACTCCAGCAG TACCGGTTGG CCATGGAAAA GAGCAGACAG 
GTGAAGGAGG ACTTTGACGG CCACATGCAA TACCTGAAGT CACCTGAGAT GGTGCAGAAA 
GAAGAGGAGA TGGATGAGCA GGAGAAGCAG CTCAAAGACA TCGAGACAAA GCTTGCCAGT 
ACTCCAGTTC GGACACCTTC CGCCATTGGG GGGAAACGGA GTCTTGACAA GGCCGGAGAA 
TCGTCCGGTA TGGTAACCAA GAGGACGAGG CGGAAGTTAT TAAAACAAGA CTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014036244.1
CDS	32..6088
Translation	MAESEGVTTSDVANSNGSHILQSKNGVNGSDKPHKSVVVFDCRPERVDAMERVLDINGLDFNDFLQVVSKEFAIPSNETFVLTTTDRKGIDFDRYEELQQGNTLHLLQTEDQILPVATKEHIVFLPHYDTLVQSGMYEYYASEGQNSLPYAFAELIDNALSATSHNTGVRTIDIRLLFDESLGKHAVVIMDNGCGMTSKQLNNWAVYRLSKFSRESSTFSSDHAGYVRPDPVARSLNSDISYFGVGGKQAVFYIGQSTRMITKPVGSPDVHELIISKEEFERKERDKEDIYSGFIRNRKMGDSSHVSEDEERFLHGLIAEEVGKDSFTVVVVTGVQPEHITFLKQDFHLWTRELAHLYHYYIHGLNGNDMKSSYRNSDCVSNIDIQISLLEKFPKIPRMVNLREIDNDMQTLYINTSADTFEFKACTDVDGIVEGILRYHPFLYDRETYPEDPYAIPATEEDDDSIVLNQARGKRPIFECFWNGRLIPYTTVSEFDWCAWPKKTGTVPAECYSRFSGVLFTNDRFQVSTNKLTFMDLELQLKNKETLFTRIVNGQEQRVKIQKEFHQWLKTCHEKWDKQVKFRGFKRTITRTDVTTKKMQSPWATFSSIEWDGRTYKAGQYVKSQKTQPIFFGSVIQFLLYGDHEGDVYATGGQVQVAMEPKALYDDIKTIPISKIDRVITNAAIKKSIDDELAKLPDKLKVTWPEGNPWTQNDVRPAGTPLGPLHVEIINKKGESISRIPSAMQNATRKALSIELKVVWHSPNGDVETNSHIAAHSAKWAFWFRTMENLNKLGKYTLHLNTILNVNESNATVWAGNRLPNHTLDFSIIEGSAERFVVGTVNTLLHVGVPFHIPLELLDEYGHPARPPPNLKPLLESSALEVSYEGVAARGTILTIRGVRARGAVQNYQSKTHDLKVVLPGLKQDSQTLKISLLPGNPDSLKVKQEDPITVENGTSVSFDVEVHDEAGNVTAHPKLIISCQLQGIPGSSSMVAVDCSNTGAGTLVLKPITFKNIVGDQTVKATFDIPSQKNVAAVQRDLRVVPSTRVSRLEVYSQEDEDSLVDSMVLRDKEKIDWTAGDLLENLHYRLYDERGGEVPLSEEMARRIKVNWTADVSVEELAQGKLPNVPVPSLVKEEHFYQVSYMEELVSVDTSFTIVPRPDEPKHMKATLSESTVRMGEVLSGKIFLQLTDQYGNKTQMLTATCVDSLIVDAEGLDKASLVFTWQESTHSMLVTGVRFNPGFPGIRELNFTWRDFTEHVTIKLTAGVPTQLKLVDGPQEPLQVLNEQGIQTPFLLQLFDEWGNPSPDQRVVVTLKTSTPALKVKSSVNSQPIDKDGKASFVVAHVSGPKSPRGEYALEFRGSFNKKPIHCLSVKLTIIPDPNKPVKLVVEYNTNSVFPAGGTLTVFSVSVVSEEGSTIKNLNPANMSMLLWKGEPSGTSRPPSGAAQLKCSKPMEDEKADRFHFRDKVIPDHVGKYTIQFVLCVDKTKGLWSHQYVINVVPNDPVKLAPDLQPPTPVVVNNNVLDSRILVEDMSLKLMDIYNNSAGLELSGKVVVTIKSAKGSSDKNLPLFEGKAKSLQFSLANGEAQITNLSIMENSPGQDGNEYVLFFRPSVPGYGPKNPLAAFELPFRFYNDVENRKQMSELTKKKDQLKQTVDIYRSLFDTNRQLITELSNQVRNATNKESHLKSELMKNGMAVAQLSSIPAIDEVIGQKTADVEKMKLQTRRVCSMPDPFRGNPDVLGKIGHLALVEDNDVATVISWHLLGDIDCVVTMTTVAARRIYDDTQGKQQVMPLDTVFWKNNSSRPLPHKRRDGQDSFRPIGNPVFARDLLIFPENAENCQIVFGNLLGDTILTDDLDSANHYRRGVVQSKMPCPTLLTRQGDRIRSNGKFGGLQNKAPPIERLRGQVFGAPLPKDYHTFMGQIDLLQQYRLAMEKSRQVKEDFDGHMQYLKSPEMVQKEEEMDEQEKQLKDIETKLASTPVRTPSAIGGKRSLDKAGESSGMVTKRTRRKLLKQDY

Target ORF information:

RefSeq Version	XM_014180769.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar structural maintenance of chromosomes flexible hinge domain containing 1 (smchd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014180769.1

ORF Insert Sequence:

ATGGCAGAGT CGGAAGGTGT GACAACGTCT GATGTTGCTA ATAGCAATGG ATCACACATT 
TTACAGAGTA AAAATGGAGT TAACGGGTCA GATAAACCAC ATAAAAGTGT GGTAGTATTC 
GACTGTCGCC CGGAAAGAGT CGACGCAATG GAGAGAGTTC TGGACATAAA TGGCTTGGAC 
TTCAACGACT TCCTTCAAGT TGTATCGAAG GAATTTGCCA TTCCTTCCAA CGAAACGTTT 
GTCTTGACAA CAACGGACAG AAAAGGAATA GATTTTGACA GATATGAGGA ACTTCAACAA 
GGAAACACTC TCCACCTGTT GCAGACCGAG GACCAGATCC TGCCCGTGGC AACTAAGGAA 
CACATTGTGT TTCTCCCTCA CTATGACACA CTGGTCCAGA GTGGCATGTA TGAGTACTAC 
GCTAGTGAGG GCCAGAATTC TTTACCGTAT GCCTTTGCAG AACTTATCGA CAATGCACTG 
TCAGCTACAT CGCACAACAC AGGAGTGAGG ACCATAGACA TCCGATTGCT GTTTGATGAG 
TCCTTGGGGA AACATGCAGT GGTTATCATG GACAATGGCT GTGGAATGAC CTCCAAGCAG 
CTTAATAACT GGGCTGTGTA CAGGCTCTCC AAATTCAGCA GGGAAAGCAG CACGTTTTCA 
AGTGATCACG CAGGGTACGT TCGCCCTGAT CCAGTCGCTC GCAGTCTCAA CAGTGATATA 
TCATATTTTG GAGTAGGAGG GAAACAAGCA GTGTTCTACA TTGGACAGTC AACCAGGATG 
ATCACCAAAC CAGTGGGCTC CCCAGATGTC CATGAACTCA TCATTTCAAA AGAAGAATTT 
GAGAGGAAGG AGCGGGACAA GGAAGATATT TACAGTGGTT TCATCAGAAA CCGGAAGATG 
GGTGACTCGT CCCACGTGAG CGAGGACGAG GAGCGCTTCC TCCACGGGCT GATCGCTGAG 
GAGGTGGGAA AGGACAGCTT CACGGTCGTG GTGGTGACGG GAGTCCAGCC CGAACACATC 
ACCTTCCTCA AGCAGGACTT CCACCTGTGG ACCAGAGAAC TGGCCCACTT GTATCACTAT 
TACATTCACG GCCTAAATGG GAATGACATG AAATCCTCCT ACCGAAACTC TGATTGTGTT 
TCCAACATTG ACATTCAGAT CTCTCTGTTG GAGAAGTTCC CCAAGATCCC TCGCATGGTG 
AACCTCAGAG AGATCGACAA CGACATGCAG ACGCTCTACA TCAACACGTC GGCTGACACA 
TTCGAGTTCA AGGCCTGCAC AGACGTTGAC GGAATAGTGG AGGGGATCCT GAGGTACCAC 
CCCTTCCTCT ATGACAGGGA GACCTACCCC GAAGACCCCT ATGCCATACC AGCTACGGAA 
GAGGATGATG ACAGTATCGT CCTGAACCAG GCCAGGGGGA AGAGGCCCAT ATTTGAGTGT 
TTTTGGAATG GACGTCTCAT CCCATACACC ACTGTATCCG AGTTCGACTG GTGCGCATGG 
CCCAAGAAGA CGGGGACAGT GCCTGCAGAG TGTTACAGCA GGTTCTCAGG GGTGCTGTTC 
ACCAATGACA GGTTCCAGGT CAGCACCAAC AAGCTCACTT TCATGGACCT GGAGCTGCAG 
CTAAAGAATA AGGAGACCTT ATTCACCCGC ATCGTCAATG GCCAGGAACA AAGGGTTAAA 
ATCCAGAAGG AGTTTCACCA GTGGCTGAAG ACCTGCCACG AGAAGTGGGA CAAGCAAGTG 
AAGTTCAGAG GCTTCAAGAG AACCATCACA CGAACAGACG TGACCACAAA GAAGATGCAA 
TCTCCTTGGG CTACCTTCTC CTCCATCGAG TGGGACGGTA GAACTTACAA AGCTGGTCAA 
TATGTGAAGT CTCAGAAAAC ACAGCCCATC TTCTTTGGCT CCGTTATTCA GTTCCTACTG 
TACGGCGATC ACGAGGGAGA TGTCTACGCC ACAGGGGGGC AGGTGCAGGT GGCCATGGAA 
CCAAAGGCGC TGTACGATGA CATCAAGACA ATACCCATCT CCAAAATCGA CAGGGTGATT 
ACCAATGCAG CCATCAAGAA GAGCATTGAT GACGAACTAG CCAAACTCCC AGACAAGCTA 
AAAGTGACCT GGCCAGAGGG CAACCCCTGG ACCCAGAACG ATGTCCGGCC AGCAGGCACT 
CCACTAGGAC CTCTCCATGT GGAAATAATC AACAAGAAAG GAGAGTCAAT ATCCAGGATA 
CCATCAGCCA TGCAAAACGC AACACGGAAA GCACTTTCAA TCGAACTAAA AGTTGTTTGG 
CATTCACCTA ATGGTGATGT GGAGACCAAC TCTCACATTG CAGCACATTC GGCCAAGTGG 
GCGTTCTGGT TCAGAACCAT GGAGAACCTG AACAAGCTGG GGAAGTACAC TCTGCACCTG 
AACACAATAC TCAATGTCAA TGAGAGCAAC GCTACTGTCT GGGCTGGGAA TCGGCTTCCC 
AACCACACAC TGGACTTCTC CATTATTGAG GGCAGTGCAG AGAGGTTTGT GGTGGGTACA 
GTGAACACCC TGCTCCATGT GGGGGTGCCT TTCCACATCC CACTGGAGCT GCTGGATGAA 
TACGGTCACC CTGCTCGCCC CCCTCCCAAC CTCAAGCCCC TCCTGGAAAG CAGTGCCCTG 
GAAGTGAGTT ACGAGGGGGT GGCCGCTAGG GGGACCATTT TGACCATCCG AGGCGTCAGA 
GCCAGGGGGG CGGTGCAGAA CTACCAGAGC AAGACGCACG ACTTGAAAGT GGTACTGCCG 
GGCCTCAAAC AGGACTCGCA GACTCTGAAG ATCAGCCTTC TCCCTGGTAA CCCAGATTCA 
CTCAAAGTGA AACAAGAGGA TCCAATCACT GTGGAGAATG GAACTTCGGT TAGCTTTGAT 
GTGGAGGTCC ATGACGAAGC AGGAAATGTC ACAGCTCACC CCAAACTGAT CATCAGCTGC 
CAGTTGCAGG GGATTCCTGG TTCATCCTCC ATGGTGGCTG TAGACTGCAG TAACACAGGA 
GCCGGGACCC TGGTGCTAAA ACCAATCACC TTCAAAAACA TCGTGGGAGA CCAGACGGTT 
AAAGCAACAT TTGATATTCC CAGTCAGAAG AATGTAGCTG CAGTCCAGAG GGATCTGAGG 
GTTGTCCCCA GCACCCGTGT GTCACGTCTC GAGGTGTATA GTCAGGAGGA CGAGGACAGC 
CTGGTGGACT CCATGGTGCT TCGGGACAAG GAAAAGATAG ACTGGACGGC AGGGGACCTC 
CTGGAGAACC TCCATTATAG ACTGTATGAC GAGAGAGGCG GGGAGGTCCC TCTGTCTGAG 
GAGATGGCCC GGCGGATCAA GGTGAACTGG ACGGCTGACG TGAGTGTGGA GGAGCTGGCC 
CAGGGGAAGC TGCCCAACGT GCCGGTCCCG TCGCTGGTGA AGGAGGAGCA CTTCTACCAG 
GTCTCCTACA TGGAGGAGCT AGTGTCTGTC GACACCTCCT TCACTATAGT GCCCCGCCCC 
GATGAGCCGA AACACATGAA GGCCACCCTG AGCGAGTCGA CCGTGAGGAT GGGAGAGGTC 
CTATCAGGAA AGATCTTCTT ACAGCTTACA GACCAGTATG GTAATAAAAC TCAGATGCTG 
ACCGCGACCT GTGTGGACTC TCTCATCGTG GATGCCGAGG GTCTCGACAA AGCATCCCTG 
GTCTTTACCT GGCAGGAGAG CACCCACTCC ATGTTAGTGA CGGGGGTGCG GTTCAACCCT 
GGGTTCCCAG GCATCAGAGA GCTGAACTTC ACCTGGAGGG ACTTTACGGA GCATGTGACC 
ATTAAATTGA CGGCCGGGGT CCCGACACAG CTCAAACTAG TGGACGGACC ACAGGAGCCC 
TTGCAGGTAC TGAATGAGCA GGGCATCCAG ACGCCCTTCC TCCTCCAGCT GTTTGACGAG 
TGGGGGAACC CCTCTCCTGA CCAGAGGGTT GTGGTGACAC TGAAGACTTC CACTCCAGCT 
CTGAAGGTGA AGTCATCCGT TAATTCACAA CCGATAGACA AAGACGGAAA AGCCAGTTTC 
GTAGTGGCCC ATGTGAGCGG TCCAAAGTCT CCCAGGGGGG AGTATGCGCT GGAGTTCAGA 
GGGTCCTTCA ACAAGAAGCC CATCCACTGT CTATCCGTAA AGCTTACCAT TATCCCCGAT 
CCTAACAAAC CTGTCAAACT GGTGGTGGAG TACAACACAA ACTCAGTGTT TCCTGCTGGA 
GGCACCCTCA CAGTGTTTTC AGTGTCTGTT GTGTCGGAGG AGGGCAGCAC CATCAAGAAC 
CTCAATCCTG CCAACATGTC CATGCTGTTG TGGAAGGGAG AGCCGTCAGG GACGTCACGG 
CCCCCCTCAG GGGCTGCCCA ACTGAAATGC AGCAAACCCA TGGAAGACGA AAAGGCGGAC 
CGTTTTCACT TCAGAGACAA AGTCATCCCA GACCATGTCG GGAAGTACAC CATCCAGTTT 
GTGCTGTGTG TTGACAAGAC TAAAGGGCTG TGGAGTCACC AGTATGTCAT AAATGTTGTA 
CCCAACGATC CTGTCAAATT GGCCCCAGAC CTGCAGCCAC CAACCCCAGT GGTGGTTAAC 
AACAACGTCC TTGACAGTCG GATTCTGGTG GAGGACATGT CTCTGAAATT AATGGATATT 
TATAATAACT CAGCGGGGCT GGAGCTGAGT GGGAAGGTGG TGGTGACCAT TAAGAGCGCC 
AAAGGCTCCA GTGACAAAAA CCTGCCTCTC TTTGAGGGCA AAGCCAAGAG CCTGCAGTTT 
AGCCTGGCTA ATGGAGAGGC CCAAATCACT AATCTGTCCA TCATGGAGAA CAGTCCTGGT 
CAGGATGGGA ATGAGTATGT CTTGTTCTTC AGACCTTCCG TCCCAGGGTA TGGCCCCAAA 
AATCCCCTGG CAGCGTTTGA GCTTCCCTTC CGCTTCTACA ACGATGTGGA GAACAGGAAG 
CAGATGTCTG AACTGACCAA GAAGAAGGAC CAGCTCAAAC AGACCGTTGA TATCTACAGG 
AGTCTGTTTG ACACCAACAG GCAACTCATC ACTGAGCTCA GTAATCAGGT GCGCAACGCC 
ACCAACAAGG AGAGTCACTT GAAATCCGAG CTCATGAAGA ATGGCATGGC TGTAGCACAA 
CTGTCATCTA TCCCAGCGAT CGACGAAGTC ATCGGTCAGA AGACGGCTGA TGTGGAGAAG 
ATGAAACTCC AGACGCGGAG AGTCTGCTCC ATGCCCGATC CCTTCAGGGG CAACCCTGAC 
GTCCTGGGAA AAATTGGCCA CCTGGCGCTG GTGGAGGACA ACGACGTGGC CACGGTCATC 
TCCTGGCACC TCTTGGGGGA CATTGACTGT GTGGTCACCA TGACAACGGT GGCGGCACGG 
AGGATCTATG ACGACACCCA GGGGAAGCAG CAGGTCATGC CCCTGGACAC GGTGTTCTGG 
AAGAACAACA GCAGCAGACC CTTGCCCCAC AAAAGGAGGG ACGGTCAGGA CAGCTTTCGT 
CCCATTGGGA ACCCTGTGTT TGCCCGAGAC CTCCTCATTT TCCCAGAGAA TGCAGAGAAC 
TGCCAGATAG TGTTCGGAAA CCTCCTTGGA GACACCATTT TGACGGATGA CCTGGACTCT 
GCTAACCACT ACAGGAGGGG GGTGGTCCAG AGCAAGATGC CATGCCCCAC CCTCCTGACC 
CGGCAGGGCG ACAGGATCCG CAGCAACGGC AAGTTTGGGG GCTTGCAGAA CAAAGCCCCT 
CCAATAGAGA GACTGCGTGG CCAGGTGTTC GGCGCACCCC TCCCCAAGGA CTACCACACC 
TTTATGGGCC AGATTGACCT ACTCCAGCAG TACCGGTTGG CCATGGAAAA GAGCAGACAG 
GTGAAGGAGG ACTTTGACGG CCACATGCAA TACCTGAAGT CACCTGAGAT GGTGCAGAAA 
GAAGAGGAGA TGGATGAGCA GGAGAAGCAG CTCAAAGACA TCGAGACAAA GCTTGCCAGT 
ACTCCAGTTC GGACACCTTC CGCCATTGGG GGGAAACGGA GTCTTGACAA GGCCGGAGAA 
TCGTCCGGTA TGGTAACCAA GAGGACGAGG CGGAAGTTAT TAAAACAAGA CTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smchd1 ( XM_014180770.1 ) cDNA ORF clone, Salmo salar(Atlantic salmon) -> XP_014036245.1 Salmo salar structural maintenance of chromosomes flexible hinge domain containing 1 (smchd1), transcript variant X2, mRNA.

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CloneID	OAt73893
Clone ID Related Accession (Same CDS sequence)	XM_014180770.1
Accession Version	XM_014180770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442678400000
Organism	Salmo salar(Atlantic salmon)
Product	structural maintenance of chromosomes flexible hinge domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012341707.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Salmo salar Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGT CGGAAGGTGT GACAACGTCT GATGTTGCTA ATAGCAATGG ATCACACATT 
TTACAGAGTA AAAATGGAGT TAACGGGTCA GATAAACCAC ATAAAAGTGT GGTAGTATTC 
GACTGTCGCC CGGAAAGAGT CGACGCAATG GAGAGAGTTC TGGACATAAA TGGCTTGGAC 
TTCAACGACT TCCTTCAAGT TGTATCGAAG GAATTTGCCA TTCCTTCCAA CGAAACGTTT 
GTCTTGACAA CAACGGACAG AAAAGGAATA GATTTTGACA GATATGAGGA ACTTCAACAA 
GGAAACACTC TCCACCTGTT GCAGACCGAG GACCAGATCC TGCCCGTGGC AACTAAGGAA 
CACATTGTGT TTCTCCCTCA CTATGACACA CTGGTCCAGA GTGGCATGTA TGAGTACTAC 
GCTAGTGAGG GCCAGAATTC TTTACCGTAT GCCTTTGCAG AACTTATCGA CAATGCACTG 
TCAGCTACAT CGCACAACAC AGGAGTGAGG ACCATAGACA TCCGATTGCT GTTTGATGAG 
TCCTTGGGGA AACATGCAGT GGTTATCATG GACAATGGCT GTGGAATGAC CTCCAAGCAG 
CTTAATAACT GGGCTGTGTA CAGGCTCTCC AAATTCAGCA GGGAAAGCAG CACGTTTTCA 
AGTGATCACG CAGGGTACGT TCGCCCTGAT CCAGTCGCTC GCAGTCTCAA CAGTGATATA 
TCATATTTTG GAGTAGGAGG GAAACAAGCA GTGTTCTACA TTGGACAGTC AACCAGGATG 
ATCACCAAAC CAGTGGGCTC CCCAGATGTC CATGAACTCA TCATTTCAAA AGAAGAATTT 
GAGAGGAAGG AGCGGGACAA GGAAGATATT TACAGTGGTT TCATCAGAAA CCGGAAGATG 
GGTGACTCGT CCCACGTGAG CGAGGACGAG GAGCGCTTCC TCCACGGGCT GATCGCTGAG 
GAGGTGGGAA AGGACAGCTT CACGGTCGTG GTGGTGACGG GAGTCCAGCC CGAACACATC 
ACCTTCCTCA AGCAGGACTT CCACCTGTGG ACCAGAGAAC TGGCCCACTT GTATCACTAT 
TACATTCACG GCCTAAATGG GAATGACATG AAATCCTCCT ACCGAAACTC TGATTGTGTT 
TCCAACATTG ACATTCAGAT CTCTCTGTTG GAGAAGTTCC CCAAGATCCC TCGCATGGTG 
AACCTCAGAG AGATCGACAA CGACATGCAG ACGCTCTACA TCAACACGTC GGCTGACACA 
TTCGAGTTCA AGGCCTGCAC AGACGTTGAC GGAATAGTGG AGGGGATCCT GAGGTACCAC 
CCCTTCCTCT ATGACAGGGA GACCTACCCC GAAGACCCCT ATGCCATACC AGCTACGGAA 
GAGGATGATG ACAGTATCGT CCTGAACCAG GCCAGGGGGA AGAGGCCCAT ATTTGAGTGT 
TTTTGGAATG GACGTCTCAT CCCATACACC ACTGTATCCG AGTTCGACTG GTGCGCATGG 
CCCAAGAAGA CGGGGACAGT GCCTGCAGAG TGTTACAGCA GGTTCTCAGG GGTGCTGTTC 
ACCAATGACA GGTTCCAGGT CAGCACCAAC AAGCTCACTT TCATGGACCT GGAGCTGCAG 
CTAAAGAATA AGGAGACCTT ATTCACCCGC ATCGTCAATG GCCAGGAACA AAGGGTTAAA 
ATCCAGAAGG AGTTTCACCA GTGGCTGAAG ACCTGCCACG AGAAGTGGGA CAAGCAAGTG 
AAGTTCAGAG GCTTCAAGAG AACCATCACA CGAACAGACG TGACCACAAA GAAGATGCAA 
TCTCCTTGGG CTACCTTCTC CTCCATCGAG TGGGACGGTA GAACTTACAA AGCTGGTCAA 
TATGTGAAGT CTCAGAAAAC ACAGCCCATC TTCTTTGGCT CCGTTATTCA GTTCCTACTG 
TACGGCGATC ACGAGGGAGA TGTCTACGCC ACAGGGGGGC AGGTGCAGGT GGCCATGGAA 
CCAAAGGCGC TGTACGATGA CATCAAGACA ATACCCATCT CCAAAATCGA CAGGGTGATT 
ACCAATGCAG CCATCAAGAA GAGCATTGAT GACGAACTAG CCAAACTCCC AGACAAGCTA 
AAAGTGACCT GGCCAGAGGG CAACCCCTGG ACCCAGAACG ATGTCCGGCC AGCAGGCACT 
CCACTAGGAC CTCTCCATGT GGAAATAATC AACAAGAAAG GAGAGTCAAT ATCCAGGATA 
CCATCAGCCA TGCAAAACGC AACACGGAAA GCACTTTCAA TCGAACTAAA AGTTGTTTGG 
CATTCACCTA ATGGTGATGT GGAGACCAAC TCTCACATTG CAGCACATTC GGCCAAGTGG 
GCGTTCTGGT TCAGAACCAT GGAGAACCTG AACAAGCTGG GGAAGTACAC TCTGCACCTG 
AACACAATAC TCAATGTCAA TGAGAGCAAC GCTACTGTCT GGGCTGGGAA TCGGCTTCCC 
AACCACACAC TGGACTTCTC CATTATTGAG GGCAGTGCAG AGAGGTTTGT GGTGGGTACA 
GTGAACACCC TGCTCCATGT GGGGGTGCCT TTCCACATCC CACTGGAGCT GCTGGATGAA 
TACGGTCACC CTGCTCGCCC CCCTCCCAAC CTCAAGCCCC TCCTGGAAAG CAGTGCCCTG 
GAAGTGAGTT ACGAGGGGGT GGCCGCTAGG GGGACCATTT TGACCATCCG AGGCGTCAGA 
GCCAGGGGGG CGGTGCAGAA CTACCAGAGC AAGACGCACG ACTTGAAAGT GGTACTGCCG 
GGCCTCAAAC AGGACTCGCA GACTCTGAAG ATCAGCCTTC TCCCTGGTAA CCCAGATTCA 
CTCAAAGTGA AACAAGAGGA TCCAATCACT GTGGAGAATG GAACTTCGGT TAGCTTTGAT 
GTGGAGGTCC ATGACGAAGC AGGAAATGTC ACAGCTCACC CCAAACTGAT CATCAGCTGC 
CAGTTGCAGG GGATTCCTGG TTCATCCTCC ATGGTGGCTG TAGACTGCAG TAACACAGGA 
GCCGGGACCC TGGTGCTAAA ACCAATCACC TTCAAAAACA TCGTGGGAGA CCAGACGGTT 
AAAGCAACAT TTGATATTCC CAGTCAGAAG AATGTAGCTG CAGTCCAGAG GGATCTGAGG 
GTTGTCCCCA GCACCCGTGT GTCACGTCTC GAGGTGTATA GTCAGGAGGA CGAGGACAGC 
CTGGTGGACT CCATGGTGCT TCGGGACAAG GAAAAGATAG ACTGGACGGC AGGGGACCTC 
CTGGAGAACC TCCATTATAG ACTGTATGAC GAGAGAGGCG GGGAGGTCCC TCTGTCTGAG 
GAGATGGCCC GGCGGATCAA GGTGAACTGG ACGGCTGACG TGAGTGTGGA GGAGCTGGCC 
CAGGGGAAGC TGCCCAACGT GCCGGTCCCG TCGCTGGTGA AGGAGGAGCA CTTCTACCAG 
GTCTCCTACA TGGAGGAGCT AGTGTCTGTC GACACCTCCT TCACTATAGT GCCCCGCCCC 
GATGAGCCGA AACACATGAA GGCCACCCTG AGCGAGTCGA CCGTGAGGAT GGGAGAGGTC 
CTATCAGGAA AGATCTTCTT ACAGCTTACA GACCAGTATG GTAATAAAAC TCAGATGCTG 
ACCGCGACCT GTGTGGACTC TCTCATCGTG GATGCCGAGG GTCTCGACAA AGCATCCCTG 
GTCTTTACCT GGCAGGAGAG CACCCACTCC ATGTTAGTGA CGGGGGTGCG GTTCAACCCT 
GGGTTCCCAG GCATCAGAGA GCTGAACTTC ACCTGGAGGG ACTTTACGGA GCATGTGACC 
ATTAAATTGA CGGCCGGGGT CCCGACACAG CTCAAACTAG TGGACGGACC ACAGGAGCCC 
TTGCAGGTAC TGAATGAGCA GGGCATCCAG ACGCCCTTCC TCCTCCAGCT GTTTGACGAG 
TGGGGGAACC CCTCTCCTGA CCAGAGGGTT GTGGTGACAC TGAAGACTTC CACTCCAGCT 
CTGAAGGTGA AGTCATCCGT TAATTCACAA CCGATAGACA AAGACGGAAA AGCCAGTTTC 
GTAGTGGCCC ATGTGAGCGG TCCAAAGGGG GAGTATGCGC TGGAGTTCAG AGGGTCCTTC 
AACAAGAAGC CCATCCACTG TCTATCCGTA AAGCTTACCA TTATCCCCGA TCCTAACAAA 
CCTGTCAAAC TGGTGGTGGA GTACAACACA AACTCAGTGT TTCCTGCTGG AGGCACCCTC 
ACAGTGTTTT CAGTGTCTGT TGTGTCGGAG GAGGGCAGCA CCATCAAGAA CCTCAATCCT 
GCCAACATGT CCATGCTGTT GTGGAAGGGA GAGCCGTCAG GGACGTCACG GCCCCCCTCA 
GGGGCTGCCC AACTGAAATG CAGCAAACCC ATGGAAGACG AAAAGGCGGA CCGTTTTCAC 
TTCAGAGACA AAGTCATCCC AGACCATGTC GGGAAGTACA CCATCCAGTT TGTGCTGTGT 
GTTGACAAGA CTAAAGGGCT GTGGAGTCAC CAGTATGTCA TAAATGTTGT ACCCAACGAT 
CCTGTCAAAT TGGCCCCAGA CCTGCAGCCA CCAACCCCAG TGGTGGTTAA CAACAACGTC 
CTTGACAGTC GGATTCTGGT GGAGGACATG TCTCTGAAAT TAATGGATAT TTATAATAAC 
TCAGCGGGGC TGGAGCTGAG TGGGAAGGTG GTGGTGACCA TTAAGAGCGC CAAAGGCTCC 
AGTGACAAAA ACCTGCCTCT CTTTGAGGGC AAAGCCAAGA GCCTGCAGTT TAGCCTGGCT 
AATGGAGAGG CCCAAATCAC TAATCTGTCC ATCATGGAGA ACAGTCCTGG TCAGGATGGG 
AATGAGTATG TCTTGTTCTT CAGACCTTCC GTCCCAGGGT ATGGCCCCAA AAATCCCCTG 
GCAGCGTTTG AGCTTCCCTT CCGCTTCTAC AACGATGTGG AGAACAGGAA GCAGATGTCT 
GAACTGACCA AGAAGAAGGA CCAGCTCAAA CAGACCGTTG ATATCTACAG GAGTCTGTTT 
GACACCAACA GGCAACTCAT CACTGAGCTC AGTAATCAGG TGCGCAACGC CACCAACAAG 
GAGAGTCACT TGAAATCCGA GCTCATGAAG AATGGCATGG CTGTAGCACA ACTGTCATCT 
ATCCCAGCGA TCGACGAAGT CATCGGTCAG AAGACGGCTG ATGTGGAGAA GATGAAACTC 
CAGACGCGGA GAGTCTGCTC CATGCCCGAT CCCTTCAGGG GCAACCCTGA CGTCCTGGGA 
AAAATTGGCC ACCTGGCGCT GGTGGAGGAC AACGACGTGG CCACGGTCAT CTCCTGGCAC 
CTCTTGGGGG ACATTGACTG TGTGGTCACC ATGACAACGG TGGCGGCACG GAGGATCTAT 
GACGACACCC AGGGGAAGCA GCAGGTCATG CCCCTGGACA CGGTGTTCTG GAAGAACAAC 
AGCAGCAGAC CCTTGCCCCA CAAAAGGAGG GACGGTCAGG ACAGCTTTCG TCCCATTGGG 
AACCCTGTGT TTGCCCGAGA CCTCCTCATT TTCCCAGAGA ATGCAGAGAA CTGCCAGATA 
GTGTTCGGAA ACCTCCTTGG AGACACCATT TTGACGGATG ACCTGGACTC TGCTAACCAC 
TACAGGAGGG GGGTGGTCCA GAGCAAGATG CCATGCCCCA CCCTCCTGAC CCGGCAGGGC 
GACAGGATCC GCAGCAACGG CAAGTTTGGG GGCTTGCAGA ACAAAGCCCC TCCAATAGAG 
AGACTGCGTG GCCAGGTGTT CGGCGCACCC CTCCCCAAGG ACTACCACAC CTTTATGGGC 
CAGATTGACC TACTCCAGCA GTACCGGTTG GCCATGGAAA AGAGCAGACA GGTGAAGGAG 
GACTTTGACG GCCACATGCA ATACCTGAAG TCACCTGAGA TGGTGCAGAA AGAAGAGGAG 
ATGGATGAGC AGGAGAAGCA GCTCAAAGAC ATCGAGACAA AGCTTGCCAG TACTCCAGTT 
CGGACACCTT CCGCCATTGG GGGGAAACGG AGTCTTGACA AGGCCGGAGA ATCGTCCGGT 
ATGGTAACCA AGAGGACGAG GCGGAAGTTA TTAAAACAAG ACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014036245.1
CDS	32..6079
Translation	MAESEGVTTSDVANSNGSHILQSKNGVNGSDKPHKSVVVFDCRPERVDAMERVLDINGLDFNDFLQVVSKEFAIPSNETFVLTTTDRKGIDFDRYEELQQGNTLHLLQTEDQILPVATKEHIVFLPHYDTLVQSGMYEYYASEGQNSLPYAFAELIDNALSATSHNTGVRTIDIRLLFDESLGKHAVVIMDNGCGMTSKQLNNWAVYRLSKFSRESSTFSSDHAGYVRPDPVARSLNSDISYFGVGGKQAVFYIGQSTRMITKPVGSPDVHELIISKEEFERKERDKEDIYSGFIRNRKMGDSSHVSEDEERFLHGLIAEEVGKDSFTVVVVTGVQPEHITFLKQDFHLWTRELAHLYHYYIHGLNGNDMKSSYRNSDCVSNIDIQISLLEKFPKIPRMVNLREIDNDMQTLYINTSADTFEFKACTDVDGIVEGILRYHPFLYDRETYPEDPYAIPATEEDDDSIVLNQARGKRPIFECFWNGRLIPYTTVSEFDWCAWPKKTGTVPAECYSRFSGVLFTNDRFQVSTNKLTFMDLELQLKNKETLFTRIVNGQEQRVKIQKEFHQWLKTCHEKWDKQVKFRGFKRTITRTDVTTKKMQSPWATFSSIEWDGRTYKAGQYVKSQKTQPIFFGSVIQFLLYGDHEGDVYATGGQVQVAMEPKALYDDIKTIPISKIDRVITNAAIKKSIDDELAKLPDKLKVTWPEGNPWTQNDVRPAGTPLGPLHVEIINKKGESISRIPSAMQNATRKALSIELKVVWHSPNGDVETNSHIAAHSAKWAFWFRTMENLNKLGKYTLHLNTILNVNESNATVWAGNRLPNHTLDFSIIEGSAERFVVGTVNTLLHVGVPFHIPLELLDEYGHPARPPPNLKPLLESSALEVSYEGVAARGTILTIRGVRARGAVQNYQSKTHDLKVVLPGLKQDSQTLKISLLPGNPDSLKVKQEDPITVENGTSVSFDVEVHDEAGNVTAHPKLIISCQLQGIPGSSSMVAVDCSNTGAGTLVLKPITFKNIVGDQTVKATFDIPSQKNVAAVQRDLRVVPSTRVSRLEVYSQEDEDSLVDSMVLRDKEKIDWTAGDLLENLHYRLYDERGGEVPLSEEMARRIKVNWTADVSVEELAQGKLPNVPVPSLVKEEHFYQVSYMEELVSVDTSFTIVPRPDEPKHMKATLSESTVRMGEVLSGKIFLQLTDQYGNKTQMLTATCVDSLIVDAEGLDKASLVFTWQESTHSMLVTGVRFNPGFPGIRELNFTWRDFTEHVTIKLTAGVPTQLKLVDGPQEPLQVLNEQGIQTPFLLQLFDEWGNPSPDQRVVVTLKTSTPALKVKSSVNSQPIDKDGKASFVVAHVSGPKGEYALEFRGSFNKKPIHCLSVKLTIIPDPNKPVKLVVEYNTNSVFPAGGTLTVFSVSVVSEEGSTIKNLNPANMSMLLWKGEPSGTSRPPSGAAQLKCSKPMEDEKADRFHFRDKVIPDHVGKYTIQFVLCVDKTKGLWSHQYVINVVPNDPVKLAPDLQPPTPVVVNNNVLDSRILVEDMSLKLMDIYNNSAGLELSGKVVVTIKSAKGSSDKNLPLFEGKAKSLQFSLANGEAQITNLSIMENSPGQDGNEYVLFFRPSVPGYGPKNPLAAFELPFRFYNDVENRKQMSELTKKKDQLKQTVDIYRSLFDTNRQLITELSNQVRNATNKESHLKSELMKNGMAVAQLSSIPAIDEVIGQKTADVEKMKLQTRRVCSMPDPFRGNPDVLGKIGHLALVEDNDVATVISWHLLGDIDCVVTMTTVAARRIYDDTQGKQQVMPLDTVFWKNNSSRPLPHKRRDGQDSFRPIGNPVFARDLLIFPENAENCQIVFGNLLGDTILTDDLDSANHYRRGVVQSKMPCPTLLTRQGDRIRSNGKFGGLQNKAPPIERLRGQVFGAPLPKDYHTFMGQIDLLQQYRLAMEKSRQVKEDFDGHMQYLKSPEMVQKEEEMDEQEKQLKDIETKLASTPVRTPSAIGGKRSLDKAGESSGMVTKRTRRKLLKQDY

Target ORF information:

RefSeq Version	XM_014180770.1
Organism	Salmo salar(Atlantic salmon)
Definition	Salmo salar structural maintenance of chromosomes flexible hinge domain containing 1 (smchd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014180770.1

ORF Insert Sequence:

ATGGCAGAGT CGGAAGGTGT GACAACGTCT GATGTTGCTA ATAGCAATGG ATCACACATT 
TTACAGAGTA AAAATGGAGT TAACGGGTCA GATAAACCAC ATAAAAGTGT GGTAGTATTC 
GACTGTCGCC CGGAAAGAGT CGACGCAATG GAGAGAGTTC TGGACATAAA TGGCTTGGAC 
TTCAACGACT TCCTTCAAGT TGTATCGAAG GAATTTGCCA TTCCTTCCAA CGAAACGTTT 
GTCTTGACAA CAACGGACAG AAAAGGAATA GATTTTGACA GATATGAGGA ACTTCAACAA 
GGAAACACTC TCCACCTGTT GCAGACCGAG GACCAGATCC TGCCCGTGGC AACTAAGGAA 
CACATTGTGT TTCTCCCTCA CTATGACACA CTGGTCCAGA GTGGCATGTA TGAGTACTAC 
GCTAGTGAGG GCCAGAATTC TTTACCGTAT GCCTTTGCAG AACTTATCGA CAATGCACTG 
TCAGCTACAT CGCACAACAC AGGAGTGAGG ACCATAGACA TCCGATTGCT GTTTGATGAG 
TCCTTGGGGA AACATGCAGT GGTTATCATG GACAATGGCT GTGGAATGAC CTCCAAGCAG 
CTTAATAACT GGGCTGTGTA CAGGCTCTCC AAATTCAGCA GGGAAAGCAG CACGTTTTCA 
AGTGATCACG CAGGGTACGT TCGCCCTGAT CCAGTCGCTC GCAGTCTCAA CAGTGATATA 
TCATATTTTG GAGTAGGAGG GAAACAAGCA GTGTTCTACA TTGGACAGTC AACCAGGATG 
ATCACCAAAC CAGTGGGCTC CCCAGATGTC CATGAACTCA TCATTTCAAA AGAAGAATTT 
GAGAGGAAGG AGCGGGACAA GGAAGATATT TACAGTGGTT TCATCAGAAA CCGGAAGATG 
GGTGACTCGT CCCACGTGAG CGAGGACGAG GAGCGCTTCC TCCACGGGCT GATCGCTGAG 
GAGGTGGGAA AGGACAGCTT CACGGTCGTG GTGGTGACGG GAGTCCAGCC CGAACACATC 
ACCTTCCTCA AGCAGGACTT CCACCTGTGG ACCAGAGAAC TGGCCCACTT GTATCACTAT 
TACATTCACG GCCTAAATGG GAATGACATG AAATCCTCCT ACCGAAACTC TGATTGTGTT 
TCCAACATTG ACATTCAGAT CTCTCTGTTG GAGAAGTTCC CCAAGATCCC TCGCATGGTG 
AACCTCAGAG AGATCGACAA CGACATGCAG ACGCTCTACA TCAACACGTC GGCTGACACA 
TTCGAGTTCA AGGCCTGCAC AGACGTTGAC GGAATAGTGG AGGGGATCCT GAGGTACCAC 
CCCTTCCTCT ATGACAGGGA GACCTACCCC GAAGACCCCT ATGCCATACC AGCTACGGAA 
GAGGATGATG ACAGTATCGT CCTGAACCAG GCCAGGGGGA AGAGGCCCAT ATTTGAGTGT 
TTTTGGAATG GACGTCTCAT CCCATACACC ACTGTATCCG AGTTCGACTG GTGCGCATGG 
CCCAAGAAGA CGGGGACAGT GCCTGCAGAG TGTTACAGCA GGTTCTCAGG GGTGCTGTTC 
ACCAATGACA GGTTCCAGGT CAGCACCAAC AAGCTCACTT TCATGGACCT GGAGCTGCAG 
CTAAAGAATA AGGAGACCTT ATTCACCCGC ATCGTCAATG GCCAGGAACA AAGGGTTAAA 
ATCCAGAAGG AGTTTCACCA GTGGCTGAAG ACCTGCCACG AGAAGTGGGA CAAGCAAGTG 
AAGTTCAGAG GCTTCAAGAG AACCATCACA CGAACAGACG TGACCACAAA GAAGATGCAA 
TCTCCTTGGG CTACCTTCTC CTCCATCGAG TGGGACGGTA GAACTTACAA AGCTGGTCAA 
TATGTGAAGT CTCAGAAAAC ACAGCCCATC TTCTTTGGCT CCGTTATTCA GTTCCTACTG 
TACGGCGATC ACGAGGGAGA TGTCTACGCC ACAGGGGGGC AGGTGCAGGT GGCCATGGAA 
CCAAAGGCGC TGTACGATGA CATCAAGACA ATACCCATCT CCAAAATCGA CAGGGTGATT 
ACCAATGCAG CCATCAAGAA GAGCATTGAT GACGAACTAG CCAAACTCCC AGACAAGCTA 
AAAGTGACCT GGCCAGAGGG CAACCCCTGG ACCCAGAACG ATGTCCGGCC AGCAGGCACT 
CCACTAGGAC CTCTCCATGT GGAAATAATC AACAAGAAAG GAGAGTCAAT ATCCAGGATA 
CCATCAGCCA TGCAAAACGC AACACGGAAA GCACTTTCAA TCGAACTAAA AGTTGTTTGG 
CATTCACCTA ATGGTGATGT GGAGACCAAC TCTCACATTG CAGCACATTC GGCCAAGTGG 
GCGTTCTGGT TCAGAACCAT GGAGAACCTG AACAAGCTGG GGAAGTACAC TCTGCACCTG 
AACACAATAC TCAATGTCAA TGAGAGCAAC GCTACTGTCT GGGCTGGGAA TCGGCTTCCC 
AACCACACAC TGGACTTCTC CATTATTGAG GGCAGTGCAG AGAGGTTTGT GGTGGGTACA 
GTGAACACCC TGCTCCATGT GGGGGTGCCT TTCCACATCC CACTGGAGCT GCTGGATGAA 
TACGGTCACC CTGCTCGCCC CCCTCCCAAC CTCAAGCCCC TCCTGGAAAG CAGTGCCCTG 
GAAGTGAGTT ACGAGGGGGT GGCCGCTAGG GGGACCATTT TGACCATCCG AGGCGTCAGA 
GCCAGGGGGG CGGTGCAGAA CTACCAGAGC AAGACGCACG ACTTGAAAGT GGTACTGCCG 
GGCCTCAAAC AGGACTCGCA GACTCTGAAG ATCAGCCTTC TCCCTGGTAA CCCAGATTCA 
CTCAAAGTGA AACAAGAGGA TCCAATCACT GTGGAGAATG GAACTTCGGT TAGCTTTGAT 
GTGGAGGTCC ATGACGAAGC AGGAAATGTC ACAGCTCACC CCAAACTGAT CATCAGCTGC 
CAGTTGCAGG GGATTCCTGG TTCATCCTCC ATGGTGGCTG TAGACTGCAG TAACACAGGA 
GCCGGGACCC TGGTGCTAAA ACCAATCACC TTCAAAAACA TCGTGGGAGA CCAGACGGTT 
AAAGCAACAT TTGATATTCC CAGTCAGAAG AATGTAGCTG CAGTCCAGAG GGATCTGAGG 
GTTGTCCCCA GCACCCGTGT GTCACGTCTC GAGGTGTATA GTCAGGAGGA CGAGGACAGC 
CTGGTGGACT CCATGGTGCT TCGGGACAAG GAAAAGATAG ACTGGACGGC AGGGGACCTC 
CTGGAGAACC TCCATTATAG ACTGTATGAC GAGAGAGGCG GGGAGGTCCC TCTGTCTGAG 
GAGATGGCCC GGCGGATCAA GGTGAACTGG ACGGCTGACG TGAGTGTGGA GGAGCTGGCC 
CAGGGGAAGC TGCCCAACGT GCCGGTCCCG TCGCTGGTGA AGGAGGAGCA CTTCTACCAG 
GTCTCCTACA TGGAGGAGCT AGTGTCTGTC GACACCTCCT TCACTATAGT GCCCCGCCCC 
GATGAGCCGA AACACATGAA GGCCACCCTG AGCGAGTCGA CCGTGAGGAT GGGAGAGGTC 
CTATCAGGAA AGATCTTCTT ACAGCTTACA GACCAGTATG GTAATAAAAC TCAGATGCTG 
ACCGCGACCT GTGTGGACTC TCTCATCGTG GATGCCGAGG GTCTCGACAA AGCATCCCTG 
GTCTTTACCT GGCAGGAGAG CACCCACTCC ATGTTAGTGA CGGGGGTGCG GTTCAACCCT 
GGGTTCCCAG GCATCAGAGA GCTGAACTTC ACCTGGAGGG ACTTTACGGA GCATGTGACC 
ATTAAATTGA CGGCCGGGGT CCCGACACAG CTCAAACTAG TGGACGGACC ACAGGAGCCC 
TTGCAGGTAC TGAATGAGCA GGGCATCCAG ACGCCCTTCC TCCTCCAGCT GTTTGACGAG 
TGGGGGAACC CCTCTCCTGA CCAGAGGGTT GTGGTGACAC TGAAGACTTC CACTCCAGCT 
CTGAAGGTGA AGTCATCCGT TAATTCACAA CCGATAGACA AAGACGGAAA AGCCAGTTTC 
GTAGTGGCCC ATGTGAGCGG TCCAAAGGGG GAGTATGCGC TGGAGTTCAG AGGGTCCTTC 
AACAAGAAGC CCATCCACTG TCTATCCGTA AAGCTTACCA TTATCCCCGA TCCTAACAAA 
CCTGTCAAAC TGGTGGTGGA GTACAACACA AACTCAGTGT TTCCTGCTGG AGGCACCCTC 
ACAGTGTTTT CAGTGTCTGT TGTGTCGGAG GAGGGCAGCA CCATCAAGAA CCTCAATCCT 
GCCAACATGT CCATGCTGTT GTGGAAGGGA GAGCCGTCAG GGACGTCACG GCCCCCCTCA 
GGGGCTGCCC AACTGAAATG CAGCAAACCC ATGGAAGACG AAAAGGCGGA CCGTTTTCAC 
TTCAGAGACA AAGTCATCCC AGACCATGTC GGGAAGTACA CCATCCAGTT TGTGCTGTGT 
GTTGACAAGA CTAAAGGGCT GTGGAGTCAC CAGTATGTCA TAAATGTTGT ACCCAACGAT 
CCTGTCAAAT TGGCCCCAGA CCTGCAGCCA CCAACCCCAG TGGTGGTTAA CAACAACGTC 
CTTGACAGTC GGATTCTGGT GGAGGACATG TCTCTGAAAT TAATGGATAT TTATAATAAC 
TCAGCGGGGC TGGAGCTGAG TGGGAAGGTG GTGGTGACCA TTAAGAGCGC CAAAGGCTCC 
AGTGACAAAA ACCTGCCTCT CTTTGAGGGC AAAGCCAAGA GCCTGCAGTT TAGCCTGGCT 
AATGGAGAGG CCCAAATCAC TAATCTGTCC ATCATGGAGA ACAGTCCTGG TCAGGATGGG 
AATGAGTATG TCTTGTTCTT CAGACCTTCC GTCCCAGGGT ATGGCCCCAA AAATCCCCTG 
GCAGCGTTTG AGCTTCCCTT CCGCTTCTAC AACGATGTGG AGAACAGGAA GCAGATGTCT 
GAACTGACCA AGAAGAAGGA CCAGCTCAAA CAGACCGTTG ATATCTACAG GAGTCTGTTT 
GACACCAACA GGCAACTCAT CACTGAGCTC AGTAATCAGG TGCGCAACGC CACCAACAAG 
GAGAGTCACT TGAAATCCGA GCTCATGAAG AATGGCATGG CTGTAGCACA ACTGTCATCT 
ATCCCAGCGA TCGACGAAGT CATCGGTCAG AAGACGGCTG ATGTGGAGAA GATGAAACTC 
CAGACGCGGA GAGTCTGCTC CATGCCCGAT CCCTTCAGGG GCAACCCTGA CGTCCTGGGA 
AAAATTGGCC ACCTGGCGCT GGTGGAGGAC AACGACGTGG CCACGGTCAT CTCCTGGCAC 
CTCTTGGGGG ACATTGACTG TGTGGTCACC ATGACAACGG TGGCGGCACG GAGGATCTAT 
GACGACACCC AGGGGAAGCA GCAGGTCATG CCCCTGGACA CGGTGTTCTG GAAGAACAAC 
AGCAGCAGAC CCTTGCCCCA CAAAAGGAGG GACGGTCAGG ACAGCTTTCG TCCCATTGGG 
AACCCTGTGT TTGCCCGAGA CCTCCTCATT TTCCCAGAGA ATGCAGAGAA CTGCCAGATA 
GTGTTCGGAA ACCTCCTTGG AGACACCATT TTGACGGATG ACCTGGACTC TGCTAACCAC 
TACAGGAGGG GGGTGGTCCA GAGCAAGATG CCATGCCCCA CCCTCCTGAC CCGGCAGGGC 
GACAGGATCC GCAGCAACGG CAAGTTTGGG GGCTTGCAGA ACAAAGCCCC TCCAATAGAG 
AGACTGCGTG GCCAGGTGTT CGGCGCACCC CTCCCCAAGG ACTACCACAC CTTTATGGGC 
CAGATTGACC TACTCCAGCA GTACCGGTTG GCCATGGAAA AGAGCAGACA GGTGAAGGAG 
GACTTTGACG GCCACATGCA ATACCTGAAG TCACCTGAGA TGGTGCAGAA AGAAGAGGAG 
ATGGATGAGC AGGAGAAGCA GCTCAAAGAC ATCGAGACAA AGCTTGCCAG TACTCCAGTT 
CGGACACCTT CCGCCATTGG GGGGAAACGG AGTCTTGACA AGGCCGGAGA ATCGTCCGGT 
ATGGTAACCA AGAGGACGAG GCGGAAGTTA TTAAAACAAG ACTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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smchd1 cDNA ORF clone, Salmo salar(Atlantic salmon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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