ORF » Species Summary » Saccharomyces cerevisiae S288C » BRF1 cDNA ORF clone

BRF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following BRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02460 NM_001181375.1
Latest version!		 Saccharomyces cerevisiae S288C transcription factor TFIIIB subunit BRF1 (BRF1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    BRF1 ( NM_001181375.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011762.1 Saccharomyces cerevisiae S288C transcription factor TFIIIB subunit BRF1 (BRF1), partial mRNA.
    Price & Availability↑
    CloneID OSi02460
    Clone ID Related Accession (Same CDS sequence) NM_001181375.1
    Accession Version NM_001181375.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1572451200000
    Organism Saccharomyces cerevisiae S288C
    Product transcription factor TFIIIB subunit BRF1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCCAGTGT GTAAGAACTG TCACGGAACC GAATTTGAGA GAGATCTTTC CAATGCTAAC 
    AACGATTTAG TTTGTAAAGC TTGTGGTGTT GTCTCAGAAG ATAATCCTAT CGTCTCTGAA 
    GTTACATTCG GTGAAACAAG TGCAGGTGCT GCTGTAGTAC AAGGTTCTTT TATCGGTGCA 
    GGACAAAGTC ATGCTGCATT TGGTGGTTCT AGTGCTCTAG AATCTAGAGA AGCCACATTG 
    AACAATGCAA GAAGGAAATT ACGCGCCGTT TCTTACGCAT TACATATCCC GGAATATATC 
    ACAGATGCTG CTTTCCAATG GTATAAGCTT GCATTGGCTA ATAACTTTGT ACAAGGTCGT 
    AGGTCCCAAA ATGTTATCGC TTCATGTCTT TACGTTGCAT GTAGAAAGGA AAAGACGCAT 
    CATATGCTGA TCGACTTCTC TTCAAGGTTA CAGGTGAGTG TGTATTCCAT AGGAGCCACA 
    TTTTTGAAAA TGGTAAAGAA ATTACATATC ACAGAATTGC CGTTAGCGGA TCCTTCCTTA 
    TTCATTCAAC ATTTTGCCGA AAAATTGGAT CTTGCTGACA AGAAGATCAA AGTGGTAAAA 
    GATGCCGTAA AATTGGCTCA AAGAATGTCC AAGGACTGGA TGTTTGAAGG ACGTAGACCT 
    GCAGGTATTG CCGGCGCATG TATTCTACTA GCTTGCAGGA TGAATAATTT GAGAAGAACT 
    CATACAGAGA TCGTCGCAGT TTCACATGTC GCCGAGGAAA CTTTACAGCA ACGGTTGAAC 
    GAATTCAAAA ATACAAAGGC TGCTAAACTT TCAGTTCAAA AATTTAGAGA AAATGATGTG 
    GAAGACGGTG AGGCTAGGCC TCCCTCCTTT GTGAAGAACA GAAAGAAAGA AAGAAAAATA 
    AAGGACTCTT TAGACAAAGA AGAGATGTTT CAAACAAGTG AAGAAGCATT GAATAAAAAT 
    CCAATTTTGA CGCAAGTATT AGGAGAACAA GAACTGTCTT CCAAGGAAGT CCTGTTTTAC 
    TTAAAACAAT TTTCAGAAAG AAGGGCCCGT GTTGTGGAGA GGATTAAGGC TACTAATGGT 
    ATAGATGGTG AAAATATATA CCATGAAGGT TCTGAAAACG AAACAAGGAA ACGCAAACTT 
    TCTGAAGTTT CTATCCAGAA CGAGCATGTT GAAGGGGAAG ACAAAGAGAC GGAAGGGACT 
    GAGGAAAAAG TCAAGAAGGT AAAGACAAAA ACATCAGAAG AAAAGAAAGA GAACGAATCT 
    GGGCATTTTC AAGATGCCAT TGATGGTTAC TCTCTAGAAA CAGACCCATA TTGTCCTCGC 
    AATTTACACT TGTTACCCAC AACGGACACA TACCTTTCGA AAGTTAGTGA CGACCCAGAT 
    AATTTAGAAG ATGTTGATGA TGAGGAGTTA AACGCGCACC TATTAAACGA GGAAGCTTCT 
    AAATTGAAGG AAAGAATTTG GATTGGTCTG AACGCAGATT TCTTACTTGA ACAAGAGAGT 
    AAACGTTTGA AACAGGAGGC AGATATTGCC ACAGGTAACA CATCAGTGAA AAAAAAACGT 
    ACGAGACGTA GGAATAATAC TAGAAGTGAC GAACCTACAA AAACTGTGGA TGCAGCAGCA 
    GCTATCGGAT TGATGTCAGA CTTACAAGAT AAGTCTGGTT TACACGCTGC ATTGAAGGCA 
    GCTGAGGAAA GTGGTGATTT CACAACTGCT GATAGTGTCA AGAACATGTT GCAGAAGGCA 
    AGTTTCTCCA AGAAGATTAA TTACGACGCC ATTGACGGTT TGTTTAGGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_011762.1
    CDS1..1791
    Translation

    Target ORF information:

    RefSeq Version NM_001181375.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C transcription factor TFIIIB subunit BRF1 (BRF1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001181375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCCAGTGT GTAAGAACTG TCACGGAACC GAATTTGAGA GAGATCTTTC CAATGCTAAC 
    AACGATTTAG TTTGTAAAGC TTGTGGTGTT GTCTCAGAAG ATAATCCTAT CGTCTCTGAA 
    GTTACATTCG GTGAAACAAG TGCAGGTGCT GCTGTAGTAC AAGGTTCTTT TATCGGTGCA 
    GGACAAAGTC ATGCTGCATT TGGTGGTTCT AGTGCTCTAG AATCTAGAGA AGCCACATTG 
    AACAATGCAA GAAGGAAATT ACGCGCCGTT TCTTACGCAT TACATATCCC GGAATATATC 
    ACAGATGCTG CTTTCCAATG GTATAAGCTT GCATTGGCTA ATAACTTTGT ACAAGGTCGT 
    AGGTCCCAAA ATGTTATCGC TTCATGTCTT TACGTTGCAT GTAGAAAGGA AAAGACGCAT 
    CATATGCTGA TCGACTTCTC TTCAAGGTTA CAGGTGAGTG TGTATTCCAT AGGAGCCACA 
    TTTTTGAAAA TGGTAAAGAA ATTACATATC ACAGAATTGC CGTTAGCGGA TCCTTCCTTA 
    TTCATTCAAC ATTTTGCCGA AAAATTGGAT CTTGCTGACA AGAAGATCAA AGTGGTAAAA 
    GATGCCGTAA AATTGGCTCA AAGAATGTCC AAGGACTGGA TGTTTGAAGG ACGTAGACCT 
    GCAGGTATTG CCGGCGCATG TATTCTACTA GCTTGCAGGA TGAATAATTT GAGAAGAACT 
    CATACAGAGA TCGTCGCAGT TTCACATGTC GCCGAGGAAA CTTTACAGCA ACGGTTGAAC 
    GAATTCAAAA ATACAAAGGC TGCTAAACTT TCAGTTCAAA AATTTAGAGA AAATGATGTG 
    GAAGACGGTG AGGCTAGGCC TCCCTCCTTT GTGAAGAACA GAAAGAAAGA AAGAAAAATA 
    AAGGACTCTT TAGACAAAGA AGAGATGTTT CAAACAAGTG AAGAAGCATT GAATAAAAAT 
    CCAATTTTGA CGCAAGTATT AGGAGAACAA GAACTGTCTT CCAAGGAAGT CCTGTTTTAC 
    TTAAAACAAT TTTCAGAAAG AAGGGCCCGT GTTGTGGAGA GGATTAAGGC TACTAATGGT 
    ATAGATGGTG AAAATATATA CCATGAAGGT TCTGAAAACG AAACAAGGAA ACGCAAACTT 
    TCTGAAGTTT CTATCCAGAA CGAGCATGTT GAAGGGGAAG ACAAAGAGAC GGAAGGGACT 
    GAGGAAAAAG TCAAGAAGGT AAAGACAAAA ACATCAGAAG AAAAGAAAGA GAACGAATCT 
    GGGCATTTTC AAGATGCCAT TGATGGTTAC TCTCTAGAAA CAGACCCATA TTGTCCTCGC 
    AATTTACACT TGTTACCCAC AACGGACACA TACCTTTCGA AAGTTAGTGA CGACCCAGAT 
    AATTTAGAAG ATGTTGATGA TGAGGAGTTA AACGCGCACC TATTAAACGA GGAAGCTTCT 
    AAATTGAAGG AAAGAATTTG GATTGGTCTG AACGCAGATT TCTTACTTGA ACAAGAGAGT 
    AAACGTTTGA AACAGGAGGC AGATATTGCC ACAGGTAACA CATCAGTGAA AAAAAAACGT 
    ACGAGACGTA GGAATAATAC TAGAAGTGAC GAACCTACAA AAACTGTGGA TGCAGCAGCA 
    GCTATCGGAT TGATGTCAGA CTTACAAGAT AAGTCTGGTT TACACGCTGC ATTGAAGGCA 
    GCTGAGGAAA GTGGTGATTT CACAACTGCT GATAGTGTCA AGAACATGTT GCAGAAGGCA 
    AGTTTCTCCA AGAAGATTAA TTACGACGCC ATTGACGGTT TGTTTAGGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
    Nature387(6632 Suppl)81-4(1997 May)
    Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Brückner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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