LOC7453835 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC7453835
Entrez Gene ID	7453835
Full Name	NADH-dependent glutamate synthase family protein
Synonyms	POPTRDRAFT_824538
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002321983.3	XP_002322019.3	glutamate synthase [NADH], amyloplastic isoform X1
XM_002321400.2	XP_002321436.2	NADH-dependent glutamate synthase family protein
XM_002321983.2	XP_002322019.2	hypothetical protein
XM_024586183.1	XP_024441951.1	glutamate synthase [NADH], amyloplastic isoform X2
XM_024586184.1	XP_024441952.1	glutamate synthase [NADH], amyloplastic isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC7453835 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC7453835 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa444172	XM_024586183.1 Latest version!	Populus trichocarpa glutamate synthase [NADH], amyloplastic (LOC7453835), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa444173	XM_024586184.1 Latest version!	Populus trichocarpa glutamate synthase [NADH], amyloplastic (LOC7453835), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa31060	XM_002321400.2 Latest version!	Populus trichocarpa NADH-dependent glutamate synthase family protein (POPTR_0015s01950g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPa31061	XM_002321983.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0015s01950g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OPa444171	XM_002321983.3 Latest version!	Populus trichocarpa glutamate synthase [NADH], amyloplastic (LOC7453835), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC7453835 ( XM_024586183.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024441951.1 Populus trichocarpa glutamate synthase [NADH], amyloplastic (LOC7453835), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPa444172
Clone ID Related Accession (Same CDS sequence)	XM_024586183.1
Accession Version	XM_024586183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	glutamate synthase [NADH], amyloplastic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037299.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAATT GGATGCCATG GGTACACTCA CGATTTTCAA CAAATACATT TCCTAGCTGG 
GACCGTGCAC AACCCATGCG TGTCTTGGGC CATAATGGGG AAATCAACAC GCTAAGAGGC 
AATGTAAACT GGATGAAGGC TCGCGAGGGC CTAATAAAGT GCAAGGAGCT CGGTCTCTCG 
AAGAATGAGA TGAAGAAGCT TCTTCCAATT GTAGATGCTA GTTCTTCCGA CTCAGGGGCT 
TTTGATGGTG TGCTGGAGCT TTTAATTCGA GCTGGGAGAA GTCTCCCTGA AGCTGTCATG 
ATGATGATTC CTGAAGCCTG GCAAAATGAC AAGAATATGG ATCCTCAAAG GAGGGCACTG 
TACGAATACT TCTCAGCTCT TATGGAACCG TGGGATGGGC CTGCACTTAT CTCATTTACT 
GATGGCCACT ACTTGGGAGC TACTCTGGAT CGCAATGGGT TACGTCCAGG AAGATTTTAT 
GTTACACGAA GTGGACGAGT TATCATGGCT AGTGAAGTTG GTGTTGTGGA TATTCCTCCT 
GAAGATGTCC TTAGGAAAGG AAGACTTAAC CCTGGAATGA TGCTTCTGGT GGATTTTGAG 
AAGCATATTA TTGTAGATGA TGAAGCCTTG AAGCAACAAT ATTCCCTAGC AAGGCCCTAT 
GGAGAGTGGC TGAAGAGGCA AAAGATTGAA CTCAGTGACA TAGTTGACTC GGTTCAAGAA 
TCTGAGAGAG TTGCTCCAGC CATTTCTGGG GTTGTTCCTG CATCTGATGA TGACACCAGC 
ATGCAGAATA TGGGCACTCA TGGTTTGCTG GCACCATTAA AAGCTTTTGG CTATACTGTT 
GAAGCCTTGG AGATGCTGAT GCTTCCCATG GCAAAGGACG CTACAGAGGC TCTTGGTTCG 
ATGGGAAATG ATGCTCCCTT GGCTGTCATG TCTAACAGGG AAAAACTCAC TTTCGAGTAC 
TTCAAGCAAA TGTTTGCTCA AGTGACAAAC CCTCCAATTG ATCCCATCAG AGAGAAGATT 
GTCACTTCCA TGGAATGCAT GATTGGGCCA GAAGGTGATT TGACAGAAAC AACTGAAGAA 
CAATGCCACC GTCTCTCTCT AAAAGGCCCT CTTTTATCAA TTGAGCAAAT GGAAGCAATG 
AAAAAAATGA ACTTTAGCGG TTGGCGAAGC AAAGTTCTCG ACATAACTTA TTCAAAGGAA 
CGGGGTAGGA AGGGATTGGA GGAGACTTTA GATAGGATTT GTGCTGAAGC TCACGAGGCA 
ATCAAGGAAG GTTATACTGT GTTGGTTCTT TCTGACAGAG CCTTCTCATC AAAGCGTGTT 
GCTGCAAGCT CCCTCTTGGC TGTTGGCGCT GTCCACCAAT ATCTAGTAAA AAAGCTTGAG 
CGAACTCAAG TCGGGTTGAT AGTTGAATCT GCTGAACCAC GTGAAGTGCA CCATTTCTGT 
ACGTTGGTTG GTTTTGGCGC AGATGCTATC TGCCCATACT TAGCTGTAGA GGCCATTTGG 
AGATTACAGG TTGATGGCAA GATCCCACCA AAATCCACTG GCGAGTTCCA CACAAAGGAT 
GAGCTAGTCA AGAAGTATTT CAAGGCAAGC AACTATGGCA TGATGAAAGT GCTTGCCAAA 
ATGGGAATTT CAACTTTGGC CTCCTACAAG GGTGCTCAGA TTTTTGAAGG CCTGGGCCTT 
TCATCAGAAG TGATTGACAA GTGCTTTGCA GGAACTCCAA GCAGGGTAGA AGGAGCAACA 
TTTGAGATGC TTGCCCGTGA TTCACTTCAT TTGCATGAGT TGGCATTTCC TTCCCGTGTT 
CTCCCTCCTG GAAGTGCAGA AGCTGTAGCA CTGCCCAATC CGGGGGATTA TCATTGGAGA 
AAAGGTGGTG AGATTCACCT TAATGATCCT CTTGCTATTG CAAAGCTTCA AGAAGCTGCC 
AGAGGTAATA GTGTGGCTGC CTATAAGGAA TATTCTAAAC GTGTTCAGGA GTTAAACAAA 
GCTTGTAATT TGCGTGGGCT TTTGAAGTTC AAAGAGGCAG ATGTGAAAGT TTCTTTGGAT 
GAAGTAGAAC CAGCCAGTGA GATTGTTAAA AGGTTCTGTA CTGGTGCAAT GAGTTATGGA 
TCAATATCAT TGGAGGCACA CACCACACTG GCTCAGGCTA TGAACAAAAT TGGAGGAAAA 
TCAAACACAG GTGAGGGAGG TGAACAACCA TCTCGGATGG AGACTCTTCC TGATGGTTCA 
ATGAACCCAA AGAGGAGTGC AATTAAGCAG GTTGCAAGTG GGAGATTTGG AGTGTCAAGT 
TACTATCTTA CAAATGCTGA TGAGCTGCAG ATAAAGATGG CTCAGGGTGC CAAGCCTGGT 
GAAGGAGGTG AGCTTCCTGG CCACAAGGTC ATAGGAGACA TTGCAATTAC CAGAAATTCT 
ACTGCAGGGG TGGGCCTCAT TAGTCCTCCT CCCCATCATG ATATCTATTC AATTGAAGAC 
CTTGCTCAAT TGATTCACGA TCTCAAGAAT GCCAATCCAG CAGCCAGAAT AAGTGTTAAG 
TTGGTATCTG AAGCTGGTGT CGGAGTCATT GCCAGTGGGG TGGTAAAGGG ACATGCTGAC 
CATGTTTTGA TCTCAGGTCA TGATGGAGGT ACAGGTGCTT CTCGATGGAC TGGCATCAAG 
AACGCTGGGC TCCCATGGGA ACTTGGTTTG GCTGAGACCC ATCAAACACT TGTTGCCAAT 
GACCTTCGTG GCCGAACAGT TCTTCAGACT GATGGCCAAC TAAAAACTGG ACGAGATGTG 
GCCATTGCAG CCCTTCTTGG CGCAGAAGAG TTTGGTTTCA GCACTGCACC CCTTATAACA 
CTTGGTTGCA TCATGATGCG AAAGTGCCAT AAGAACACCT GCCCCGTAGG CATTGCTACT 
CAAGATCCAG TGCTGAGGGA AAAGTTTGCT GGAGAACCTG AACATGTTAT CAACTTCTTT 
TTTATGCTAG CAGAGGAGCT CAGGGAGATT ATGGCACAGC TTGGTTTCCG TACGATGACT 
GAGATGGTTG GTCGTTCAGA CATGCTTGAA GTTGATAAAG AAGTTGTTAA GAGCAACGAG 
AAGCTGGAAA ATATTGATCT CTCTTTGTTA CTTAGACCTG CTGCTGACAT TCGACCTGAA 
GCTGCTCAGT ATTGTGTCCA AAAGCAGGAT CATGGTTTGG ACATGGCATT GGATAATAAA 
CTCATTAAAC TCTCTGAGGC TGCATTGGAA AAAGGTCTTC CTGTATACAT TGAAACACCT 
ATCTGCAATG TGAACCGTGC TGTTGGAACG ATGCTTAGTC ATGAAGTTAC TAAGCGTTAC 
CACTTGGCAG GGCTTCCCGC AGATACTATT CACATCAAAC TCACAGGAAG TGCTGGGCAG 
AGTCTAGGAG CTTTCCTTTG CCCTGGCATT ATGCTGGAAC TGGAAGGGGA TGGCAATGAC 
TACGTTGGTA AAGGATTATC AGGTGGGAAG ATTGTAGTTT ATCCTCCAAA AGGAAGCCTG 
TTTGATCCAA AAGAAAACAT TGTGATCGGT AATGTAGCTC TTTATGGAGC CACATGTGGT 
GAAGCATATT TCAATGGGAT GGCAGCAGAA AGATTTTGTG TCCGTAATTC TGGGGCAAGG 
GCTGTTGTAG AAGGTGTTGG TGATCATGGC TGTGAGTACA TGACAGGTGG AACTGTTGTT 
GTGCTTGGGA AAACTGGAAG GAATTTTGCT GCTGGTATGA GTGGTGGTGT TGCTTATGTT 
CTTGATTTGG ATGGGAAATT CAGATCTCGT TGCAATCCTG AACTCGTAGA TCTTGACAAA 
GTCGAGGAAG AGGAGGACAT TACAACTCTA AAAATGATGA TACAACAACA CCAGCGTCAC 
ACAAATAGCC TGCTAGCTAG AGAAGTACTT GCTGATTTTG ATAATCTTCT ACCTAAATTT 
ATTAAGGTCT TCCCGAGGGA TTACAAACGT GTTCTTGCCA ACATGAAAGA GGAATCTGCC 
ACAAAAGAGG CTGCTGACCT AGCTGCTAAA GAAGTTGAGG AAGCTGAGGA GCAAGATGAA 
GCAGAGTTGA AGGAGAAAGA TGCTTTTGAG GAGCTAAAGA AGCTGGCAGC TGCATCTTTG 
AATGGGAATT CCATTCAGGT AGAAGATGGA CCTCTGAAAA GGCCTACCCG TGTTAATGAT 
GCTGTTAAAC ATCGAGGGTT TATTGCTTAT GAGCGAGAAG GTGTTCAGTA CAGAGATCCA 
AATATTCGAA TGAATGACTG GAAGGAAGTT ACTGAGGAGT CAAAACCTGG CCCTCTTTTA 
AAGACCCAGT CAGCCCGTTG CATGGACTGT GGCACTCCTT TCTGTCACCA GGAGAACTCA 
GGATGCCCTC TTGGAAACAA AATCCCTGAA TTTAATGAGC TAGTGCATCA AAATCGGTGG 
CGTGAAGCAT TAGATAGGTT ACTTGAGACA AATAACTTCC CAGAGTTCAC TGGCAGAGTG 
TGCCCTGCAC CCTGTGAAGG TTCCTGTGTT CTTGGGATAA TTGATGATCC AGTATCTATA 
AAAAACATTG AATGTTCAAT TATTGACAAG GCCTTTGAGG AGGGTTGGAT GGTTCCGCGG 
CCTCCCCTCA AGAGAACTGG GAGACGAGTG GCTATTGTTG GAAGCGGGCC TTCTGGTTTG 
GCTGCTGCTG ATCAACTAAA CAAAATGGGT CATTTGGTGA CTGTGTATGA GCGTGCTGAT 
CGAATTGGAG GGCTAATGAT GTATGGAGTT CCCAACATGA AAACTGACAA AGTGGATATA 
GTTCAACGGC GTGTTAACCT TATGTCTGAG GAAGGCATCA ATTTTGTGGT AAATGCTAAT 
GTTGGAATTG ATCCTTTGTA TTCTCTTGAT CGGCTTCGAG ATGAGAACAA TGCGATTGTT 
TTGGCAGTGG GAGCCACAAA ACCAAGGGAC CTTCCGGTAC CTGGTCGGGA GTTGTCAGGC 
GTCCATTTTG CTATGCAGTT TCTTCATGCA AATACCAAAA GTTTGCTTGA CAGCAATTTG 
CAGGATGGTA ACTACATATC CGCAAAGGGC AAGAAAGTTG TTGTCATTGG TGGAGGTGAC 
ACTGGAACAG ATTGCATTGG GACATCTATT CGCCATGGGT GTAGTAGCAT TGTAAATCTA 
GAGCTTCTAC CTGAGCCACC ACGAACTAGA GGTCCGGGCA ACCCTTGGCC ACAGTGGCCT 
AGAGTATTCC GGGTAGACTA TGGGCACCAG GAAGCTGCCG CCAAGTTCGG GAAAGACCCG 
AGGTCTTATG AGGTGTTGAC TAAGCGTTTC ATTGGAGATG AGAATGGGAA TGTGAAAGGA 
CTTGAGCTGG TACGTGTCCA TTGGGAGAAG GATGCCACAG GGAAATTTCA ATTTAAGGAG 
GTTGAGGGCT CCGAGGAAGT TATTGAGGCT GACCTAGTCC TACTAGCAAT GGGATTCCTT 
GGTCCTGAGC TGAATGTGGC AGAGAAGTTG GGCTTGGAGC AAGATAATAG ATCGAATTTC 
AAGGCAGAAT ATGGCCGCTT CTCAACCAAT GTGGAAGGGA TCTTTGCAGC TGGGGATTGC 
AGGCGAGGCC AGTCCCTAGT TGTATGGGCA ATATCAGAGG GACGACAAGC TGCTTCCCAG 
GTGGACAAAT ATCTCATGAA AGAAGAGGAT GCTACCATTA ACACCGATAA TACTCAGGAT 
GACCTTGTCA AGAGGCACCA GGACCTCACC AAGAGACATC AAGACAGCAG CAAGCACACT 
GTCATGACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024441951.1
CDS	20..5731
Translation	MLNWMPWVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLIKCKELGLSKNEMKKLLPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNMDPQRRALYEYFSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTRSGRVIMASEVGVVDIPPEDVLRKGRLNPGMMLLVDFEKHIIVDDEALKQQYSLARPYGEWLKRQKIELSDIVDSVQESERVAPAISGVVPASDDDTSMQNMGTHGLLAPLKAFGYTVEALEMLMLPMAKDATEALGSMGNDAPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAMKKMNFSGWRSKVLDITYSKERGRKGLEETLDRICAEAHEAIKEGYTVLVLSDRAFSSKRVAASSLLAVGAVHQYLVKKLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPPKSTGEFHTKDELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEGLGLSSEVIDKCFAGTPSRVEGATFEMLARDSLHLHELAFPSRVLPPGSAEAVALPNPGDYHWRKGGEIHLNDPLAIAKLQEAARGNSVAAYKEYSKRVQELNKACNLRGLLKFKEADVKVSLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAQAMNKIGGKSNTGEGGEQPSRMETLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEELREIMAQLGFRTMTEMVGRSDMLEVDKEVVKSNEKLENIDLSLLLRPAADIRPEAAQYCVQKQDHGLDMALDNKLIKLSEAALEKGLPVYIETPICNVNRAVGTMLSHEVTKRYHLAGLPADTIHIKLTGSAGQSLGAFLCPGIMLELEGDGNDYVGKGLSGGKIVVYPPKGSLFDPKENIVIGNVALYGATCGEAYFNGMAAERFCVRNSGARAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGVAYVLDLDGKFRSRCNPELVDLDKVEEEEDITTLKMMIQQHQRHTNSLLAREVLADFDNLLPKFIKVFPRDYKRVLANMKEESATKEAADLAAKEVEEAEEQDEAELKEKDAFEELKKLAAASLNGNSIQVEDGPLKRPTRVNDAVKHRGFIAYEREGVQYRDPNIRMNDWKEVTEESKPGPLLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVHQNRWREALDRLLETNNFPEFTGRVCPAPCEGSCVLGIIDDPVSIKNIECSIIDKAFEEGWMVPRPPLKRTGRRVAIVGSGPSGLAAADQLNKMGHLVTVYERADRIGGLMMYGVPNMKTDKVDIVQRRVNLMSEEGINFVVNANVGIDPLYSLDRLRDENNAIVLAVGATKPRDLPVPGRELSGVHFAMQFLHANTKSLLDSNLQDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCSSIVNLELLPEPPRTRGPGNPWPQWPRVFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGNVKGLELVRVHWEKDATGKFQFKEVEGSEEVIEADLVLLAMGFLGPELNVAEKLGLEQDNRSNFKAEYGRFSTNVEGIFAAGDCRRGQSLVVWAISEGRQAASQVDKYLMKEEDATINTDNTQDDLVKRHQDLTKRHQDSSKHTVMT

Target ORF information:

RefSeq Version	XM_024586183.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa glutamate synthase [NADH], amyloplastic (LOC7453835), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024586183.1

ORF Insert Sequence:

ATGCTGAATT GGATGCCATG GGTACACTCA CGATTTTCAA CAAATACATT TCCTAGCTGG 
GACCGTGCAC AACCCATGCG TGTCTTGGGC CATAATGGGG AAATCAACAC GCTAAGAGGC 
AATGTAAACT GGATGAAGGC TCGCGAGGGC CTAATAAAGT GCAAGGAGCT CGGTCTCTCG 
AAGAATGAGA TGAAGAAGCT TCTTCCAATT GTAGATGCTA GTTCTTCCGA CTCAGGGGCT 
TTTGATGGTG TGCTGGAGCT TTTAATTCGA GCTGGGAGAA GTCTCCCTGA AGCTGTCATG 
ATGATGATTC CTGAAGCCTG GCAAAATGAC AAGAATATGG ATCCTCAAAG GAGGGCACTG 
TACGAATACT TCTCAGCTCT TATGGAACCG TGGGATGGGC CTGCACTTAT CTCATTTACT 
GATGGCCACT ACTTGGGAGC TACTCTGGAT CGCAATGGGT TACGTCCAGG AAGATTTTAT 
GTTACACGAA GTGGACGAGT TATCATGGCT AGTGAAGTTG GTGTTGTGGA TATTCCTCCT 
GAAGATGTCC TTAGGAAAGG AAGACTTAAC CCTGGAATGA TGCTTCTGGT GGATTTTGAG 
AAGCATATTA TTGTAGATGA TGAAGCCTTG AAGCAACAAT ATTCCCTAGC AAGGCCCTAT 
GGAGAGTGGC TGAAGAGGCA AAAGATTGAA CTCAGTGACA TAGTTGACTC GGTTCAAGAA 
TCTGAGAGAG TTGCTCCAGC CATTTCTGGG GTTGTTCCTG CATCTGATGA TGACACCAGC 
ATGCAGAATA TGGGCACTCA TGGTTTGCTG GCACCATTAA AAGCTTTTGG CTATACTGTT 
GAAGCCTTGG AGATGCTGAT GCTTCCCATG GCAAAGGACG CTACAGAGGC TCTTGGTTCG 
ATGGGAAATG ATGCTCCCTT GGCTGTCATG TCTAACAGGG AAAAACTCAC TTTCGAGTAC 
TTCAAGCAAA TGTTTGCTCA AGTGACAAAC CCTCCAATTG ATCCCATCAG AGAGAAGATT 
GTCACTTCCA TGGAATGCAT GATTGGGCCA GAAGGTGATT TGACAGAAAC AACTGAAGAA 
CAATGCCACC GTCTCTCTCT AAAAGGCCCT CTTTTATCAA TTGAGCAAAT GGAAGCAATG 
AAAAAAATGA ACTTTAGCGG TTGGCGAAGC AAAGTTCTCG ACATAACTTA TTCAAAGGAA 
CGGGGTAGGA AGGGATTGGA GGAGACTTTA GATAGGATTT GTGCTGAAGC TCACGAGGCA 
ATCAAGGAAG GTTATACTGT GTTGGTTCTT TCTGACAGAG CCTTCTCATC AAAGCGTGTT 
GCTGCAAGCT CCCTCTTGGC TGTTGGCGCT GTCCACCAAT ATCTAGTAAA AAAGCTTGAG 
CGAACTCAAG TCGGGTTGAT AGTTGAATCT GCTGAACCAC GTGAAGTGCA CCATTTCTGT 
ACGTTGGTTG GTTTTGGCGC AGATGCTATC TGCCCATACT TAGCTGTAGA GGCCATTTGG 
AGATTACAGG TTGATGGCAA GATCCCACCA AAATCCACTG GCGAGTTCCA CACAAAGGAT 
GAGCTAGTCA AGAAGTATTT CAAGGCAAGC AACTATGGCA TGATGAAAGT GCTTGCCAAA 
ATGGGAATTT CAACTTTGGC CTCCTACAAG GGTGCTCAGA TTTTTGAAGG CCTGGGCCTT 
TCATCAGAAG TGATTGACAA GTGCTTTGCA GGAACTCCAA GCAGGGTAGA AGGAGCAACA 
TTTGAGATGC TTGCCCGTGA TTCACTTCAT TTGCATGAGT TGGCATTTCC TTCCCGTGTT 
CTCCCTCCTG GAAGTGCAGA AGCTGTAGCA CTGCCCAATC CGGGGGATTA TCATTGGAGA 
AAAGGTGGTG AGATTCACCT TAATGATCCT CTTGCTATTG CAAAGCTTCA AGAAGCTGCC 
AGAGGTAATA GTGTGGCTGC CTATAAGGAA TATTCTAAAC GTGTTCAGGA GTTAAACAAA 
GCTTGTAATT TGCGTGGGCT TTTGAAGTTC AAAGAGGCAG ATGTGAAAGT TTCTTTGGAT 
GAAGTAGAAC CAGCCAGTGA GATTGTTAAA AGGTTCTGTA CTGGTGCAAT GAGTTATGGA 
TCAATATCAT TGGAGGCACA CACCACACTG GCTCAGGCTA TGAACAAAAT TGGAGGAAAA 
TCAAACACAG GTGAGGGAGG TGAACAACCA TCTCGGATGG AGACTCTTCC TGATGGTTCA 
ATGAACCCAA AGAGGAGTGC AATTAAGCAG GTTGCAAGTG GGAGATTTGG AGTGTCAAGT 
TACTATCTTA CAAATGCTGA TGAGCTGCAG ATAAAGATGG CTCAGGGTGC CAAGCCTGGT 
GAAGGAGGTG AGCTTCCTGG CCACAAGGTC ATAGGAGACA TTGCAATTAC CAGAAATTCT 
ACTGCAGGGG TGGGCCTCAT TAGTCCTCCT CCCCATCATG ATATCTATTC AATTGAAGAC 
CTTGCTCAAT TGATTCACGA TCTCAAGAAT GCCAATCCAG CAGCCAGAAT AAGTGTTAAG 
TTGGTATCTG AAGCTGGTGT CGGAGTCATT GCCAGTGGGG TGGTAAAGGG ACATGCTGAC 
CATGTTTTGA TCTCAGGTCA TGATGGAGGT ACAGGTGCTT CTCGATGGAC TGGCATCAAG 
AACGCTGGGC TCCCATGGGA ACTTGGTTTG GCTGAGACCC ATCAAACACT TGTTGCCAAT 
GACCTTCGTG GCCGAACAGT TCTTCAGACT GATGGCCAAC TAAAAACTGG ACGAGATGTG 
GCCATTGCAG CCCTTCTTGG CGCAGAAGAG TTTGGTTTCA GCACTGCACC CCTTATAACA 
CTTGGTTGCA TCATGATGCG AAAGTGCCAT AAGAACACCT GCCCCGTAGG CATTGCTACT 
CAAGATCCAG TGCTGAGGGA AAAGTTTGCT GGAGAACCTG AACATGTTAT CAACTTCTTT 
TTTATGCTAG CAGAGGAGCT CAGGGAGATT ATGGCACAGC TTGGTTTCCG TACGATGACT 
GAGATGGTTG GTCGTTCAGA CATGCTTGAA GTTGATAAAG AAGTTGTTAA GAGCAACGAG 
AAGCTGGAAA ATATTGATCT CTCTTTGTTA CTTAGACCTG CTGCTGACAT TCGACCTGAA 
GCTGCTCAGT ATTGTGTCCA AAAGCAGGAT CATGGTTTGG ACATGGCATT GGATAATAAA 
CTCATTAAAC TCTCTGAGGC TGCATTGGAA AAAGGTCTTC CTGTATACAT TGAAACACCT 
ATCTGCAATG TGAACCGTGC TGTTGGAACG ATGCTTAGTC ATGAAGTTAC TAAGCGTTAC 
CACTTGGCAG GGCTTCCCGC AGATACTATT CACATCAAAC TCACAGGAAG TGCTGGGCAG 
AGTCTAGGAG CTTTCCTTTG CCCTGGCATT ATGCTGGAAC TGGAAGGGGA TGGCAATGAC 
TACGTTGGTA AAGGATTATC AGGTGGGAAG ATTGTAGTTT ATCCTCCAAA AGGAAGCCTG 
TTTGATCCAA AAGAAAACAT TGTGATCGGT AATGTAGCTC TTTATGGAGC CACATGTGGT 
GAAGCATATT TCAATGGGAT GGCAGCAGAA AGATTTTGTG TCCGTAATTC TGGGGCAAGG 
GCTGTTGTAG AAGGTGTTGG TGATCATGGC TGTGAGTACA TGACAGGTGG AACTGTTGTT 
GTGCTTGGGA AAACTGGAAG GAATTTTGCT GCTGGTATGA GTGGTGGTGT TGCTTATGTT 
CTTGATTTGG ATGGGAAATT CAGATCTCGT TGCAATCCTG AACTCGTAGA TCTTGACAAA 
GTCGAGGAAG AGGAGGACAT TACAACTCTA AAAATGATGA TACAACAACA CCAGCGTCAC 
ACAAATAGCC TGCTAGCTAG AGAAGTACTT GCTGATTTTG ATAATCTTCT ACCTAAATTT 
ATTAAGGTCT TCCCGAGGGA TTACAAACGT GTTCTTGCCA ACATGAAAGA GGAATCTGCC 
ACAAAAGAGG CTGCTGACCT AGCTGCTAAA GAAGTTGAGG AAGCTGAGGA GCAAGATGAA 
GCAGAGTTGA AGGAGAAAGA TGCTTTTGAG GAGCTAAAGA AGCTGGCAGC TGCATCTTTG 
AATGGGAATT CCATTCAGGT AGAAGATGGA CCTCTGAAAA GGCCTACCCG TGTTAATGAT 
GCTGTTAAAC ATCGAGGGTT TATTGCTTAT GAGCGAGAAG GTGTTCAGTA CAGAGATCCA 
AATATTCGAA TGAATGACTG GAAGGAAGTT ACTGAGGAGT CAAAACCTGG CCCTCTTTTA 
AAGACCCAGT CAGCCCGTTG CATGGACTGT GGCACTCCTT TCTGTCACCA GGAGAACTCA 
GGATGCCCTC TTGGAAACAA AATCCCTGAA TTTAATGAGC TAGTGCATCA AAATCGGTGG 
CGTGAAGCAT TAGATAGGTT ACTTGAGACA AATAACTTCC CAGAGTTCAC TGGCAGAGTG 
TGCCCTGCAC CCTGTGAAGG TTCCTGTGTT CTTGGGATAA TTGATGATCC AGTATCTATA 
AAAAACATTG AATGTTCAAT TATTGACAAG GCCTTTGAGG AGGGTTGGAT GGTTCCGCGG 
CCTCCCCTCA AGAGAACTGG GAGACGAGTG GCTATTGTTG GAAGCGGGCC TTCTGGTTTG 
GCTGCTGCTG ATCAACTAAA CAAAATGGGT CATTTGGTGA CTGTGTATGA GCGTGCTGAT 
CGAATTGGAG GGCTAATGAT GTATGGAGTT CCCAACATGA AAACTGACAA AGTGGATATA 
GTTCAACGGC GTGTTAACCT TATGTCTGAG GAAGGCATCA ATTTTGTGGT AAATGCTAAT 
GTTGGAATTG ATCCTTTGTA TTCTCTTGAT CGGCTTCGAG ATGAGAACAA TGCGATTGTT 
TTGGCAGTGG GAGCCACAAA ACCAAGGGAC CTTCCGGTAC CTGGTCGGGA GTTGTCAGGC 
GTCCATTTTG CTATGCAGTT TCTTCATGCA AATACCAAAA GTTTGCTTGA CAGCAATTTG 
CAGGATGGTA ACTACATATC CGCAAAGGGC AAGAAAGTTG TTGTCATTGG TGGAGGTGAC 
ACTGGAACAG ATTGCATTGG GACATCTATT CGCCATGGGT GTAGTAGCAT TGTAAATCTA 
GAGCTTCTAC CTGAGCCACC ACGAACTAGA GGTCCGGGCA ACCCTTGGCC ACAGTGGCCT 
AGAGTATTCC GGGTAGACTA TGGGCACCAG GAAGCTGCCG CCAAGTTCGG GAAAGACCCG 
AGGTCTTATG AGGTGTTGAC TAAGCGTTTC ATTGGAGATG AGAATGGGAA TGTGAAAGGA 
CTTGAGCTGG TACGTGTCCA TTGGGAGAAG GATGCCACAG GGAAATTTCA ATTTAAGGAG 
GTTGAGGGCT CCGAGGAAGT TATTGAGGCT GACCTAGTCC TACTAGCAAT GGGATTCCTT 
GGTCCTGAGC TGAATGTGGC AGAGAAGTTG GGCTTGGAGC AAGATAATAG ATCGAATTTC 
AAGGCAGAAT ATGGCCGCTT CTCAACCAAT GTGGAAGGGA TCTTTGCAGC TGGGGATTGC 
AGGCGAGGCC AGTCCCTAGT TGTATGGGCA ATATCAGAGG GACGACAAGC TGCTTCCCAG 
GTGGACAAAT ATCTCATGAA AGAAGAGGAT GCTACCATTA ACACCGATAA TACTCAGGAT 
GACCTTGTCA AGAGGCACCA GGACCTCACC AAGAGACATC AAGACAGCAG CAAGCACACT 
GTCATGACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7453835 ( XM_024586184.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_024441952.1 Populus trichocarpa glutamate synthase [NADH], amyloplastic (LOC7453835), transcript variant X4, mRNA.

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CloneID	OPa444173
Clone ID Related Accession (Same CDS sequence)	XM_024586184.1
Accession Version	XM_024586184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	glutamate synthase [NADH], amyloplastic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037299.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGT TGGTACACTC ACGATTTTCA ACAAATACAT TTCCTAGCTG GGACCGTGCA 
CAACCCATGC GTGTCTTGGG CCATAATGGG GAAATCAACA CGCTAAGAGG CAATGTAAAC 
TGGATGAAGG CTCGCGAGGG CCTAATAAAG TGCAAGGAGC TCGGTCTCTC GAAGAATGAG 
ATGAAGAAGC TTCTTCCAAT TGTAGATGCT AGTTCTTCCG ACTCAGGGGC TTTTGATGGT 
GTGCTGGAGC TTTTAATTCG AGCTGGGAGA AGTCTCCCTG AAGCTGTCAT GATGATGATT 
CCTGAAGCCT GGCAAAATGA CAAGAATATG GATCCTCAAA GGAGGGCACT GTACGAATAC 
TTCTCAGCTC TTATGGAACC GTGGGATGGG CCTGCACTTA TCTCATTTAC TGATGGCCAC 
TACTTGGGAG CTACTCTGGA TCGCAATGGG TTACGTCCAG GAAGATTTTA TGTTACACGA 
AGTGGACGAG TTATCATGGC TAGTGAAGTT GGTGTTGTGG ATATTCCTCC TGAAGATGTC 
CTTAGGAAAG GAAGACTTAA CCCTGGAATG ATGCTTCTGG TGGATTTTGA GAAGCATATT 
ATTGTAGATG ATGAAGCCTT GAAGCAACAA TATTCCCTAG CAAGGCCCTA TGGAGAGTGG 
CTGAAGAGGC AAAAGATTGA ACTCAGTGAC ATAGTTGACT CGGTTCAAGA ATCTGAGAGA 
GTTGCTCCAG CCATTTCTGG GGTTGTTCCT GCATCTGATG ATGACACCAG CATGCAGAAT 
ATGGGCACTC ATGGTTTGCT GGCACCATTA AAAGCTTTTG GCTATACTGT TGAAGCCTTG 
GAGATGCTGA TGCTTCCCAT GGCAAAGGAC GCTACAGAGG CTCTTGGTTC GATGGGAAAT 
GATGCTCCCT TGGCTGTCAT GTCTAACAGG GAAAAACTCA CTTTCGAGTA CTTCAAGCAA 
ATGTTTGCTC AAGTGACAAA CCCTCCAATT GATCCCATCA GAGAGAAGAT TGTCACTTCC 
ATGGAATGCA TGATTGGGCC AGAAGGTGAT TTGACAGAAA CAACTGAAGA ACAATGCCAC 
CGTCTCTCTC TAAAAGGCCC TCTTTTATCA ATTGAGCAAA TGGAAGCAAT GAAAAAAATG 
AACTTTAGCG GTTGGCGAAG CAAAGTTCTC GACATAACTT ATTCAAAGGA ACGGGGTAGG 
AAGGGATTGG AGGAGACTTT AGATAGGATT TGTGCTGAAG CTCACGAGGC AATCAAGGAA 
GGTTATACTG TGTTGGTTCT TTCTGACAGA GCCTTCTCAT CAAAGCGTGT TGCTGCAAGC 
TCCCTCTTGG CTGTTGGCGC TGTCCACCAA TATCTAGTAA AAAAGCTTGA GCGAACTCAA 
GTCGGGTTGA TAGTTGAATC TGCTGAACCA CGTGAAGTGC ACCATTTCTG TACGTTGGTT 
GGTTTTGGCG CAGATGCTAT CTGCCCATAC TTAGCTGTAG AGGCCATTTG GAGATTACAG 
GTTGATGGCA AGATCCCACC AAAATCCACT GGCGAGTTCC ACACAAAGGA TGAGCTAGTC 
AAGAAGTATT TCAAGGCAAG CAACTATGGC ATGATGAAAG TGCTTGCCAA AATGGGAATT 
TCAACTTTGG CCTCCTACAA GGGTGCTCAG ATTTTTGAAG GCCTGGGCCT TTCATCAGAA 
GTGATTGACA AGTGCTTTGC AGGAACTCCA AGCAGGGTAG AAGGAGCAAC ATTTGAGATG 
CTTGCCCGTG ATTCACTTCA TTTGCATGAG TTGGCATTTC CTTCCCGTGT TCTCCCTCCT 
GGAAGTGCAG AAGCTGTAGC ACTGCCCAAT CCGGGGGATT ATCATTGGAG AAAAGGTGGT 
GAGATTCACC TTAATGATCC TCTTGCTATT GCAAAGCTTC AAGAAGCTGC CAGAGGTAAT 
AGTGTGGCTG CCTATAAGGA ATATTCTAAA CGTGTTCAGG AGTTAAACAA AGCTTGTAAT 
TTGCGTGGGC TTTTGAAGTT CAAAGAGGCA GATGTGAAAG TTTCTTTGGA TGAAGTAGAA 
CCAGCCAGTG AGATTGTTAA AAGGTTCTGT ACTGGTGCAA TGAGTTATGG ATCAATATCA 
TTGGAGGCAC ACACCACACT GGCTCAGGCT ATGAACAAAA TTGGAGGAAA ATCAAACACA 
GGTGAGGGAG GTGAACAACC ATCTCGGATG GAGACTCTTC CTGATGGTTC AATGAACCCA 
AAGAGGAGTG CAATTAAGCA GGTTGCAAGT GGGAGATTTG GAGTGTCAAG TTACTATCTT 
ACAAATGCTG ATGAGCTGCA GATAAAGATG GCTCAGGGTG CCAAGCCTGG TGAAGGAGGT 
GAGCTTCCTG GCCACAAGGT CATAGGAGAC ATTGCAATTA CCAGAAATTC TACTGCAGGG 
GTGGGCCTCA TTAGTCCTCC TCCCCATCAT GATATCTATT CAATTGAAGA CCTTGCTCAA 
TTGATTCACG ATCTCAAGAA TGCCAATCCA GCAGCCAGAA TAAGTGTTAA GTTGGTATCT 
GAAGCTGGTG TCGGAGTCAT TGCCAGTGGG GTGGTAAAGG GACATGCTGA CCATGTTTTG 
ATCTCAGGTC ATGATGGAGG TACAGGTGCT TCTCGATGGA CTGGCATCAA GAACGCTGGG 
CTCCCATGGG AACTTGGTTT GGCTGAGACC CATCAAACAC TTGTTGCCAA TGACCTTCGT 
GGCCGAACAG TTCTTCAGAC TGATGGCCAA CTAAAAACTG GACGAGATGT GGCCATTGCA 
GCCCTTCTTG GCGCAGAAGA GTTTGGTTTC AGCACTGCAC CCCTTATAAC ACTTGGTTGC 
ATCATGATGC GAAAGTGCCA TAAGAACACC TGCCCCGTAG GCATTGCTAC TCAAGATCCA 
GTGCTGAGGG AAAAGTTTGC TGGAGAACCT GAACATGTTA TCAACTTCTT TTTTATGCTA 
GCAGAGGAGC TCAGGGAGAT TATGGCACAG CTTGGTTTCC GTACGATGAC TGAGATGGTT 
GGTCGTTCAG ACATGCTTGA AGTTGATAAA GAAGTTGTTA AGAGCAACGA GAAGCTGGAA 
AATATTGATC TCTCTTTGTT ACTTAGACCT GCTGCTGACA TTCGACCTGA AGCTGCTCAG 
TATTGTGTCC AAAAGCAGGA TCATGGTTTG GACATGGCAT TGGATAATAA ACTCATTAAA 
CTCTCTGAGG CTGCATTGGA AAAAGGTCTT CCTGTATACA TTGAAACACC TATCTGCAAT 
GTGAACCGTG CTGTTGGAAC GATGCTTAGT CATGAAGTTA CTAAGCGTTA CCACTTGGCA 
GGGCTTCCCG CAGATACTAT TCACATCAAA CTCACAGGAA GTGCTGGGCA GAGTCTAGGA 
GCTTTCCTTT GCCCTGGCAT TATGCTGGAA CTGGAAGGGG ATGGCAATGA CTACGTTGGT 
AAAGGATTAT CAGGTGGGAA GATTGTAGTT TATCCTCCAA AAGGAAGCCT GTTTGATCCA 
AAAGAAAACA TTGTGATCGG TAATGTAGCT CTTTATGGAG CCACATGTGG TGAAGCATAT 
TTCAATGGGA TGGCAGCAGA AAGATTTTGT GTCCGTAATT CTGGGGCAAG GGCTGTTGTA 
GAAGGTGTTG GTGATCATGG CTGTGAGTAC ATGACAGGTG GAACTGTTGT TGTGCTTGGG 
AAAACTGGAA GGAATTTTGC TGCTGGTATG AGTGGTGGTG TTGCTTATGT TCTTGATTTG 
GATGGGAAAT TCAGATCTCG TTGCAATCCT GAACTCGTAG ATCTTGACAA AGTCGAGGAA 
GAGGAGGACA TTACAACTCT AAAAATGATG ATACAACAAC ACCAGCGTCA CACAAATAGC 
CTGCTAGCTA GAGAAGTACT TGCTGATTTT GATAATCTTC TACCTAAATT TATTAAGGTC 
TTCCCGAGGG ATTACAAACG TGTTCTTGCC AACATGAAAG AGGAATCTGC CACAAAAGAG 
GCTGCTGACC TAGCTGCTAA AGAAGTTGAG GAAGCTGAGG AGCAAGATGA AGCAGAGTTG 
AAGGAGAAAG ATGCTTTTGA GGAGCTAAAG AAGCTGGCAG CTGCATCTTT GAATGGGAAT 
TCCATTCAGG TAGAAGATGG ACCTCTGAAA AGGCCTACCC GTGTTAATGA TGCTGTTAAA 
CATCGAGGGT TTATTGCTTA TGAGCGAGAA GGTGTTCAGT ACAGAGATCC AAATATTCGA 
ATGAATGACT GGAAGGAAGT TACTGAGGAG TCAAAACCTG GCCCTCTTTT AAAGACCCAG 
TCAGCCCGTT GCATGGACTG TGGCACTCCT TTCTGTCACC AGGAGAACTC AGGATGCCCT 
CTTGGAAACA AAATCCCTGA ATTTAATGAG CTAGTGCATC AAAATCGGTG GCGTGAAGCA 
TTAGATAGGT TACTTGAGAC AAATAACTTC CCAGAGTTCA CTGGCAGAGT GTGCCCTGCA 
CCCTGTGAAG GTTCCTGTGT TCTTGGGATA ATTGATGATC CAGTATCTAT AAAAAACATT 
GAATGTTCAA TTATTGACAA GGCCTTTGAG GAGGGTTGGA TGGTTCCGCG GCCTCCCCTC 
AAGAGAACTG GGAGACGAGT GGCTATTGTT GGAAGCGGGC CTTCTGGTTT GGCTGCTGCT 
GATCAACTAA ACAAAATGGG TCATTTGGTG ACTGTGTATG AGCGTGCTGA TCGAATTGGA 
GGGCTAATGA TGTATGGAGT TCCCAACATG AAAACTGACA AAGTGGATAT AGTTCAACGG 
CGTGTTAACC TTATGTCTGA GGAAGGCATC AATTTTGTGG TAAATGCTAA TGTTGGAATT 
GATCCTTTGT ATTCTCTTGA TCGGCTTCGA GATGAGAACA ATGCGATTGT TTTGGCAGTG 
GGAGCCACAA AACCAAGGGA CCTTCCGGTA CCTGGTCGGG AGTTGTCAGG CGTCCATTTT 
GCTATGCAGT TTCTTCATGC AAATACCAAA AGTTTGCTTG ACAGCAATTT GCAGGATGGT 
AACTACATAT CCGCAAAGGG CAAGAAAGTT GTTGTCATTG GTGGAGGTGA CACTGGAACA 
GATTGCATTG GGACATCTAT TCGCCATGGG TGTAGTAGCA TTGTAAATCT AGAGCTTCTA 
CCTGAGCCAC CACGAACTAG AGGTCCGGGC AACCCTTGGC CACAGTGGCC TAGAGTATTC 
CGGGTAGACT ATGGGCACCA GGAAGCTGCC GCCAAGTTCG GGAAAGACCC GAGGTCTTAT 
GAGGTGTTGA CTAAGCGTTT CATTGGAGAT GAGAATGGGA ATGTGAAAGG ACTTGAGCTG 
GTACGTGTCC ATTGGGAGAA GGATGCCACA GGGAAATTTC AATTTAAGGA GGTTGAGGGC 
TCCGAGGAAG TTATTGAGGC TGACCTAGTC CTACTAGCAA TGGGATTCCT TGGTCCTGAG 
CTGAATGTGG CAGAGAAGTT GGGCTTGGAG CAAGATAATA GATCGAATTT CAAGGCAGAA 
TATGGCCGCT TCTCAACCAA TGTGGAAGGG ATCTTTGCAG CTGGGGATTG CAGGCGAGGC 
CAGTCCCTAG TTGTATGGGC AATATCAGAG GGACGACAAG CTGCTTCCCA GGTGGACAAA 
TATCTCATGA AAGAAGAGGA TGCTACCATT AACACCGATA ATACTCAGGA TGACCTTGTC 
AAGAGGCACC AGGACCTCAC CAAGAGACAT CAAGACAGCA GCAAGCACAC TGTCATGACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024441952.1
CDS	114..5816
Translation	MGELVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLIKCKELGLSKNEMKKLLPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNMDPQRRALYEYFSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTRSGRVIMASEVGVVDIPPEDVLRKGRLNPGMMLLVDFEKHIIVDDEALKQQYSLARPYGEWLKRQKIELSDIVDSVQESERVAPAISGVVPASDDDTSMQNMGTHGLLAPLKAFGYTVEALEMLMLPMAKDATEALGSMGNDAPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAMKKMNFSGWRSKVLDITYSKERGRKGLEETLDRICAEAHEAIKEGYTVLVLSDRAFSSKRVAASSLLAVGAVHQYLVKKLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPPKSTGEFHTKDELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEGLGLSSEVIDKCFAGTPSRVEGATFEMLARDSLHLHELAFPSRVLPPGSAEAVALPNPGDYHWRKGGEIHLNDPLAIAKLQEAARGNSVAAYKEYSKRVQELNKACNLRGLLKFKEADVKVSLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAQAMNKIGGKSNTGEGGEQPSRMETLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEELREIMAQLGFRTMTEMVGRSDMLEVDKEVVKSNEKLENIDLSLLLRPAADIRPEAAQYCVQKQDHGLDMALDNKLIKLSEAALEKGLPVYIETPICNVNRAVGTMLSHEVTKRYHLAGLPADTIHIKLTGSAGQSLGAFLCPGIMLELEGDGNDYVGKGLSGGKIVVYPPKGSLFDPKENIVIGNVALYGATCGEAYFNGMAAERFCVRNSGARAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGVAYVLDLDGKFRSRCNPELVDLDKVEEEEDITTLKMMIQQHQRHTNSLLAREVLADFDNLLPKFIKVFPRDYKRVLANMKEESATKEAADLAAKEVEEAEEQDEAELKEKDAFEELKKLAAASLNGNSIQVEDGPLKRPTRVNDAVKHRGFIAYEREGVQYRDPNIRMNDWKEVTEESKPGPLLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVHQNRWREALDRLLETNNFPEFTGRVCPAPCEGSCVLGIIDDPVSIKNIECSIIDKAFEEGWMVPRPPLKRTGRRVAIVGSGPSGLAAADQLNKMGHLVTVYERADRIGGLMMYGVPNMKTDKVDIVQRRVNLMSEEGINFVVNANVGIDPLYSLDRLRDENNAIVLAVGATKPRDLPVPGRELSGVHFAMQFLHANTKSLLDSNLQDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCSSIVNLELLPEPPRTRGPGNPWPQWPRVFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGNVKGLELVRVHWEKDATGKFQFKEVEGSEEVIEADLVLLAMGFLGPELNVAEKLGLEQDNRSNFKAEYGRFSTNVEGIFAAGDCRRGQSLVVWAISEGRQAASQVDKYLMKEEDATINTDNTQDDLVKRHQDLTKRHQDSSKHTVMT

Target ORF information:

RefSeq Version	XM_024586184.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa glutamate synthase [NADH], amyloplastic (LOC7453835), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024586184.1

ORF Insert Sequence:

ATGGGTGAGT TGGTACACTC ACGATTTTCA ACAAATACAT TTCCTAGCTG GGACCGTGCA 
CAACCCATGC GTGTCTTGGG CCATAATGGG GAAATCAACA CGCTAAGAGG CAATGTAAAC 
TGGATGAAGG CTCGCGAGGG CCTAATAAAG TGCAAGGAGC TCGGTCTCTC GAAGAATGAG 
ATGAAGAAGC TTCTTCCAAT TGTAGATGCT AGTTCTTCCG ACTCAGGGGC TTTTGATGGT 
GTGCTGGAGC TTTTAATTCG AGCTGGGAGA AGTCTCCCTG AAGCTGTCAT GATGATGATT 
CCTGAAGCCT GGCAAAATGA CAAGAATATG GATCCTCAAA GGAGGGCACT GTACGAATAC 
TTCTCAGCTC TTATGGAACC GTGGGATGGG CCTGCACTTA TCTCATTTAC TGATGGCCAC 
TACTTGGGAG CTACTCTGGA TCGCAATGGG TTACGTCCAG GAAGATTTTA TGTTACACGA 
AGTGGACGAG TTATCATGGC TAGTGAAGTT GGTGTTGTGG ATATTCCTCC TGAAGATGTC 
CTTAGGAAAG GAAGACTTAA CCCTGGAATG ATGCTTCTGG TGGATTTTGA GAAGCATATT 
ATTGTAGATG ATGAAGCCTT GAAGCAACAA TATTCCCTAG CAAGGCCCTA TGGAGAGTGG 
CTGAAGAGGC AAAAGATTGA ACTCAGTGAC ATAGTTGACT CGGTTCAAGA ATCTGAGAGA 
GTTGCTCCAG CCATTTCTGG GGTTGTTCCT GCATCTGATG ATGACACCAG CATGCAGAAT 
ATGGGCACTC ATGGTTTGCT GGCACCATTA AAAGCTTTTG GCTATACTGT TGAAGCCTTG 
GAGATGCTGA TGCTTCCCAT GGCAAAGGAC GCTACAGAGG CTCTTGGTTC GATGGGAAAT 
GATGCTCCCT TGGCTGTCAT GTCTAACAGG GAAAAACTCA CTTTCGAGTA CTTCAAGCAA 
ATGTTTGCTC AAGTGACAAA CCCTCCAATT GATCCCATCA GAGAGAAGAT TGTCACTTCC 
ATGGAATGCA TGATTGGGCC AGAAGGTGAT TTGACAGAAA CAACTGAAGA ACAATGCCAC 
CGTCTCTCTC TAAAAGGCCC TCTTTTATCA ATTGAGCAAA TGGAAGCAAT GAAAAAAATG 
AACTTTAGCG GTTGGCGAAG CAAAGTTCTC GACATAACTT ATTCAAAGGA ACGGGGTAGG 
AAGGGATTGG AGGAGACTTT AGATAGGATT TGTGCTGAAG CTCACGAGGC AATCAAGGAA 
GGTTATACTG TGTTGGTTCT TTCTGACAGA GCCTTCTCAT CAAAGCGTGT TGCTGCAAGC 
TCCCTCTTGG CTGTTGGCGC TGTCCACCAA TATCTAGTAA AAAAGCTTGA GCGAACTCAA 
GTCGGGTTGA TAGTTGAATC TGCTGAACCA CGTGAAGTGC ACCATTTCTG TACGTTGGTT 
GGTTTTGGCG CAGATGCTAT CTGCCCATAC TTAGCTGTAG AGGCCATTTG GAGATTACAG 
GTTGATGGCA AGATCCCACC AAAATCCACT GGCGAGTTCC ACACAAAGGA TGAGCTAGTC 
AAGAAGTATT TCAAGGCAAG CAACTATGGC ATGATGAAAG TGCTTGCCAA AATGGGAATT 
TCAACTTTGG CCTCCTACAA GGGTGCTCAG ATTTTTGAAG GCCTGGGCCT TTCATCAGAA 
GTGATTGACA AGTGCTTTGC AGGAACTCCA AGCAGGGTAG AAGGAGCAAC ATTTGAGATG 
CTTGCCCGTG ATTCACTTCA TTTGCATGAG TTGGCATTTC CTTCCCGTGT TCTCCCTCCT 
GGAAGTGCAG AAGCTGTAGC ACTGCCCAAT CCGGGGGATT ATCATTGGAG AAAAGGTGGT 
GAGATTCACC TTAATGATCC TCTTGCTATT GCAAAGCTTC AAGAAGCTGC CAGAGGTAAT 
AGTGTGGCTG CCTATAAGGA ATATTCTAAA CGTGTTCAGG AGTTAAACAA AGCTTGTAAT 
TTGCGTGGGC TTTTGAAGTT CAAAGAGGCA GATGTGAAAG TTTCTTTGGA TGAAGTAGAA 
CCAGCCAGTG AGATTGTTAA AAGGTTCTGT ACTGGTGCAA TGAGTTATGG ATCAATATCA 
TTGGAGGCAC ACACCACACT GGCTCAGGCT ATGAACAAAA TTGGAGGAAA ATCAAACACA 
GGTGAGGGAG GTGAACAACC ATCTCGGATG GAGACTCTTC CTGATGGTTC AATGAACCCA 
AAGAGGAGTG CAATTAAGCA GGTTGCAAGT GGGAGATTTG GAGTGTCAAG TTACTATCTT 
ACAAATGCTG ATGAGCTGCA GATAAAGATG GCTCAGGGTG CCAAGCCTGG TGAAGGAGGT 
GAGCTTCCTG GCCACAAGGT CATAGGAGAC ATTGCAATTA CCAGAAATTC TACTGCAGGG 
GTGGGCCTCA TTAGTCCTCC TCCCCATCAT GATATCTATT CAATTGAAGA CCTTGCTCAA 
TTGATTCACG ATCTCAAGAA TGCCAATCCA GCAGCCAGAA TAAGTGTTAA GTTGGTATCT 
GAAGCTGGTG TCGGAGTCAT TGCCAGTGGG GTGGTAAAGG GACATGCTGA CCATGTTTTG 
ATCTCAGGTC ATGATGGAGG TACAGGTGCT TCTCGATGGA CTGGCATCAA GAACGCTGGG 
CTCCCATGGG AACTTGGTTT GGCTGAGACC CATCAAACAC TTGTTGCCAA TGACCTTCGT 
GGCCGAACAG TTCTTCAGAC TGATGGCCAA CTAAAAACTG GACGAGATGT GGCCATTGCA 
GCCCTTCTTG GCGCAGAAGA GTTTGGTTTC AGCACTGCAC CCCTTATAAC ACTTGGTTGC 
ATCATGATGC GAAAGTGCCA TAAGAACACC TGCCCCGTAG GCATTGCTAC TCAAGATCCA 
GTGCTGAGGG AAAAGTTTGC TGGAGAACCT GAACATGTTA TCAACTTCTT TTTTATGCTA 
GCAGAGGAGC TCAGGGAGAT TATGGCACAG CTTGGTTTCC GTACGATGAC TGAGATGGTT 
GGTCGTTCAG ACATGCTTGA AGTTGATAAA GAAGTTGTTA AGAGCAACGA GAAGCTGGAA 
AATATTGATC TCTCTTTGTT ACTTAGACCT GCTGCTGACA TTCGACCTGA AGCTGCTCAG 
TATTGTGTCC AAAAGCAGGA TCATGGTTTG GACATGGCAT TGGATAATAA ACTCATTAAA 
CTCTCTGAGG CTGCATTGGA AAAAGGTCTT CCTGTATACA TTGAAACACC TATCTGCAAT 
GTGAACCGTG CTGTTGGAAC GATGCTTAGT CATGAAGTTA CTAAGCGTTA CCACTTGGCA 
GGGCTTCCCG CAGATACTAT TCACATCAAA CTCACAGGAA GTGCTGGGCA GAGTCTAGGA 
GCTTTCCTTT GCCCTGGCAT TATGCTGGAA CTGGAAGGGG ATGGCAATGA CTACGTTGGT 
AAAGGATTAT CAGGTGGGAA GATTGTAGTT TATCCTCCAA AAGGAAGCCT GTTTGATCCA 
AAAGAAAACA TTGTGATCGG TAATGTAGCT CTTTATGGAG CCACATGTGG TGAAGCATAT 
TTCAATGGGA TGGCAGCAGA AAGATTTTGT GTCCGTAATT CTGGGGCAAG GGCTGTTGTA 
GAAGGTGTTG GTGATCATGG CTGTGAGTAC ATGACAGGTG GAACTGTTGT TGTGCTTGGG 
AAAACTGGAA GGAATTTTGC TGCTGGTATG AGTGGTGGTG TTGCTTATGT TCTTGATTTG 
GATGGGAAAT TCAGATCTCG TTGCAATCCT GAACTCGTAG ATCTTGACAA AGTCGAGGAA 
GAGGAGGACA TTACAACTCT AAAAATGATG ATACAACAAC ACCAGCGTCA CACAAATAGC 
CTGCTAGCTA GAGAAGTACT TGCTGATTTT GATAATCTTC TACCTAAATT TATTAAGGTC 
TTCCCGAGGG ATTACAAACG TGTTCTTGCC AACATGAAAG AGGAATCTGC CACAAAAGAG 
GCTGCTGACC TAGCTGCTAA AGAAGTTGAG GAAGCTGAGG AGCAAGATGA AGCAGAGTTG 
AAGGAGAAAG ATGCTTTTGA GGAGCTAAAG AAGCTGGCAG CTGCATCTTT GAATGGGAAT 
TCCATTCAGG TAGAAGATGG ACCTCTGAAA AGGCCTACCC GTGTTAATGA TGCTGTTAAA 
CATCGAGGGT TTATTGCTTA TGAGCGAGAA GGTGTTCAGT ACAGAGATCC AAATATTCGA 
ATGAATGACT GGAAGGAAGT TACTGAGGAG TCAAAACCTG GCCCTCTTTT AAAGACCCAG 
TCAGCCCGTT GCATGGACTG TGGCACTCCT TTCTGTCACC AGGAGAACTC AGGATGCCCT 
CTTGGAAACA AAATCCCTGA ATTTAATGAG CTAGTGCATC AAAATCGGTG GCGTGAAGCA 
TTAGATAGGT TACTTGAGAC AAATAACTTC CCAGAGTTCA CTGGCAGAGT GTGCCCTGCA 
CCCTGTGAAG GTTCCTGTGT TCTTGGGATA ATTGATGATC CAGTATCTAT AAAAAACATT 
GAATGTTCAA TTATTGACAA GGCCTTTGAG GAGGGTTGGA TGGTTCCGCG GCCTCCCCTC 
AAGAGAACTG GGAGACGAGT GGCTATTGTT GGAAGCGGGC CTTCTGGTTT GGCTGCTGCT 
GATCAACTAA ACAAAATGGG TCATTTGGTG ACTGTGTATG AGCGTGCTGA TCGAATTGGA 
GGGCTAATGA TGTATGGAGT TCCCAACATG AAAACTGACA AAGTGGATAT AGTTCAACGG 
CGTGTTAACC TTATGTCTGA GGAAGGCATC AATTTTGTGG TAAATGCTAA TGTTGGAATT 
GATCCTTTGT ATTCTCTTGA TCGGCTTCGA GATGAGAACA ATGCGATTGT TTTGGCAGTG 
GGAGCCACAA AACCAAGGGA CCTTCCGGTA CCTGGTCGGG AGTTGTCAGG CGTCCATTTT 
GCTATGCAGT TTCTTCATGC AAATACCAAA AGTTTGCTTG ACAGCAATTT GCAGGATGGT 
AACTACATAT CCGCAAAGGG CAAGAAAGTT GTTGTCATTG GTGGAGGTGA CACTGGAACA 
GATTGCATTG GGACATCTAT TCGCCATGGG TGTAGTAGCA TTGTAAATCT AGAGCTTCTA 
CCTGAGCCAC CACGAACTAG AGGTCCGGGC AACCCTTGGC CACAGTGGCC TAGAGTATTC 
CGGGTAGACT ATGGGCACCA GGAAGCTGCC GCCAAGTTCG GGAAAGACCC GAGGTCTTAT 
GAGGTGTTGA CTAAGCGTTT CATTGGAGAT GAGAATGGGA ATGTGAAAGG ACTTGAGCTG 
GTACGTGTCC ATTGGGAGAA GGATGCCACA GGGAAATTTC AATTTAAGGA GGTTGAGGGC 
TCCGAGGAAG TTATTGAGGC TGACCTAGTC CTACTAGCAA TGGGATTCCT TGGTCCTGAG 
CTGAATGTGG CAGAGAAGTT GGGCTTGGAG CAAGATAATA GATCGAATTT CAAGGCAGAA 
TATGGCCGCT TCTCAACCAA TGTGGAAGGG ATCTTTGCAG CTGGGGATTG CAGGCGAGGC 
CAGTCCCTAG TTGTATGGGC AATATCAGAG GGACGACAAG CTGCTTCCCA GGTGGACAAA 
TATCTCATGA AAGAAGAGGA TGCTACCATT AACACCGATA ATACTCAGGA TGACCTTGTC 
AAGAGGCACC AGGACCTCAC CAAGAGACAT CAAGACAGCA GCAAGCACAC TGTCATGACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7453835 ( XM_002321400.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002321436.2 Populus trichocarpa NADH-dependent glutamate synthase family protein (POPTR_0015s01950g) mRNA, complete cds.

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CloneID	OPa31060
Clone ID Related Accession (Same CDS sequence)	XM_002321400.2
Accession Version	XM_002321400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	NADH-dependent glutamate synthase family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008481). On Dec 16, 2013 this sequence version replaced XM_002321400.1.

ATGTCGGCTG CAGCGAGCTC TGGAGCTCTC CTTCAACCAA AATCGGTGAA AGCTCCGTTT 
TCTTCGCTTA GTAAATCGTC TTCTCTTTCT CCTTCGCTCA ATGTTGCCAC AGCAGCATCT 
GTTTCTCGGC GGAGCGCGAG GGCAAACCGA TGTGCTTCGA CGAGGAAATC GGTGGTTGTT 
GAGAGAAAAT CGTTTTTGGG AAGTAAAGTG CGGGGGTCTG CTGGATCGGA GAGATTGCAT 
TTTTGGCAGT CGGATGGACC GGGTAGAGAG CCGAAACTTC GAGTTGTTGT CCGGTCTGCA 
TTGTCCGGTG TGCCGGAGAA GCCGCTTGGG TTGTATGACC CGTCGTTTGA TAAGGATTCG 
TGCGGGGTTG GTTTTGTGGC TGAATTATCT GGGGATAGCA GTCGTAAAAC GGTAAATGAT 
GCTTTGGAGA TGTTGGTGAG GATGACACAT AGAGGGGCTT GTGGATGTGA GACGAATACT 
GGTGATGGGG CTGGTATTCT TGTGGCTCTT CCTCATGATT TCTACAAGGA GGTTGCTAAG 
GATATAGGGT TCGAACTGCC ACCACCAGGG GAATATGCAG TTGGCATGTT CTTTTTGCCC 
ACATCTGATA ATCGTAAGGA AGAAAGCAAG AATGTATTCA CTAAGGTTGC GGAGTCTCTT 
GGGCATACTG TACTTGGGTG GCGACCAGTA CCAACAGATA ACTCTGGACT GGGAAATTCT 
GCTTTGCAGA CCGAGCCTGT CATTGAGCAA GTGTTCCTTA CAGCCACTCC TAGGTCGAAG 
GCTGATTTTG AGCAGCAGAT GTACATATTA AGGAGGGTGT CAATGGTAGC TATTAGAGCT 
GCACTAAACC TTCAATATGG TGGTGTTAGG GACTTTTATA TCTGTTCCCT TTCCTCAAGG 
ACGGTTGTTT ATAAAGGTCA GTTGAAGCCT GAACAGTTGA AGGGTTACTA TTATGCAGAT 
CTTGGCAATG AAAGGTTTAC GAGCTACATG GCCCTGGTAC ACTCACGATT TTCAACAAAT 
ACATTTCCTA GCTGGGATCG TGCACAACCC ATGCGTGTCT TGGGCCATAA TGGGGAAATC 
AACACGCTAA GAGGCAATGT AAACTGGATG AAGGCTCGCG AGGGCCTAAT AAAGTGCAAG 
GAGCTCGGTC TCTCGAAGAA TGAGATGAAG AAGCTTCTTC CAATTGTAGA TGCTAGTTCT 
TCCGACTCAG GGGCTTTTGA TGGTGTGCTG GAGCTTTTAA TTCGAGCTGG GAGAAGTCTC 
CCTGAAGCTG TCATGATGAT GATTCCTGAA GCCTGGCAAA ATGACAAGAA TATGGATCCT 
CAAAGGAGGG CACTGTACGA ATACTTCTCA GCTCTTATGG AACCGTGGGA TGGGCCTGCA 
CTTATCTCAT TTACTGATGG CCACTACTTG GGAGCTACTC TGGATCGCAA TGGGTTACGT 
CCAGGAAGAT TTTATGTTAC ACGAAGTGGA CGAGTTATCA TGGCTAGTGA AGTTGGTGTT 
GTGGATATTC CTCCTGAAGA TGTCCTTAGG AAAGGAAGAC TTAACCCTGG AATGATGCTT 
CTGGTGGATT TTGAGAAGCA TATTATTGTA GATGATGAAG CCTTGAAGCA ACAATATTCC 
CTAGCAAGGC CCTATGGAGA GTGGCTGAAG AGGCAAAAGA TTGAACTCAG TGACATAGTT 
GACTCGGTTC AAGAATCTGA GAGAGTTGCT CCAGCCATTT CTGGGGTTGT TCCTGCATCT 
GATGATGACA CCAGCATGCA GAATATGGGC ACTCATGGTT TGCTGGCACC ATTAAAAGCT 
TTTGGCTATA CTGTTGAAGC CTTGGAGATG CTGATGCTTC CCATGGCAAA GGACGCTACA 
GAGGCTCTTG GTTCGATGGG AAATGATGCT CCCTTGGCTG TCATGTCTAA CAGGGAAAAA 
CTCACTTTCG AGTACTTCAA GCAAATGTTT GCTCAAGTGA CAAACCCTCC AATTGATCCC 
ATCAGAGAGA AGATTGTCAC TTCCATGGAA TGCATGATTG GGCCAGAAGG TGATTTGACA 
GAAACAACTG AAGAACAATG CCACCGTCTC TCTCTAAAAG GCCCTCTTTT ATCAATTGAG 
CAAATGGAAG CAATGAAAAA AATGAACTTT AGCGGTTGGC GAAGCAAAGT TCTCGACATA 
ACTTATTCAA AGGAACGGGG TAGGAAGGGA TTGGAGGAGA CTTTAGATAG GATTTGTGCT 
GAAGCTCACG AGGCAATCAA GGAAGGTTAT ACTGTGTTGG TTCTTTCTGA CAGAGCCTTC 
TCATCAAAGC GTGTTGCTGC AAGCTCCCTC TTGGCTGTTG GCGCTGTCCA CCAATATCTA 
GTAAAAAAGC TTGAGCGAAC TCAAGTCGGG TTGATAGTTG AATCTGCTGA ACCACGTGAA 
GTGCACCATT TCTGTACGTT GGTTGGTTTT GGCGCAGATG CTATCTGCCC ATACTTAGCT 
GTAGAGGCCA TTTGGAGATT ACAGGTTGAT GGCAAGATCC CACCAAAATC CACTGGCGAG 
TTCCACACAA AGGATGAGCT AGTCAAGAAG TATTTCAAGG CAAGCAACTA TGGCATGATG 
AAAGTGCTTG CCAAAATGGG AATTTCAACT TTGGCCTCCT ACAAGGGTGC TCAGATTTTT 
GAAGGCCTGG GCCTTTCATC AGAAGTGATT GACAAGTGCT TTGCAGGAAC TCCAAGCAGG 
GTAGAAGGAG CAACATTTGA GATGCTTGCC CGTGATTCAC TTCATTTGCA TGAGTTGGCA 
TTTCCTTCCC GTGTTCTCCC TCCTGGAAGT GCAGAAGCTG TAGCACTGCC CAATCCGGGG 
GATTATCATT GGAGAAAAGG TGGTGAGATT CACCTTAATG ATCCTCTTGC TATTGCAAAG 
CTTCAAGAAG CTGCCAGAGG TAATAGTGTG GCTGCCTATA AGGAATATTC TAAACGTGTT 
CAGGAGTTAA ACAAAGCTTG TAATTTGCGT GGGCTTTTGA AGTTCAAAGA GGCAGATGTG 
AAAGTTTCTT TGGATGAAGT AGAACCAGCC AGTGAGATTG TTAAAAGGTT CTGTACTGGT 
GCAATGAGTT ATGGATCAAT ATCATTGGAG GCACACACCA CACTGGCTCA GGCTATGAAC 
AAAATTGGAG GAAAATCAAA CACAGGTGAG GGAGGTGAAC AACCATCTCG GATGGAGACT 
CTTCCTGATG GTTCAATGAA CCCAAAGAGG AGTGCAATTA AGCAGGTTGC AAGTGGGAGA 
TTTGGAGTGT CAAGTTACTA TCTTACAAAT GCTGATGAGC TGCAGATAAA GATGGCTCAG 
GGTGCCAAGC CTGGTGAAGG AGGTGAGCTT CCTGGCCACA AGGTCATAGG AGACATTGCA 
ATTACCAGAA ATTCTACTGC AGGGGTGGGC CTCATTAGTC CTCCTCCCCA TCATGATATC 
TATTCAATTG AAGACCTTGC TCAATTGATT CACGATCTCA AGAATGCCAA TCCAGCAGCC 
AGAATAAGTG TTAAGTTGGT ATCTGAAGCT GGTGTCGGAG TCATTGCCAG TGGGGTGGTA 
AAGGGACATG CTGACCATGT TTTGATCTCA GGTCATGATG GAGGTACAGG TGCTTCTCGA 
TGGACTGGCA TCAAGAACGC TGGGCTCCCA TGGGAACTTG GTTTGGCTGA GACCCATCAA 
ACACTTGTTG CCAATGACCT TCGTGGCCGA ACAGTTCTTC AGACTGATGG CCAACTAAAA 
ACTGGACGAG ATGTGGCCAT TGCAGCCCTT CTTGGCGCAG AAGAGTTTGG TTTCAGCACT 
GCACCCCTTA TAACACTTGG TTGCATCATG ATGCGAAAGT GCCATAAGAA CACCTGCCCC 
GTAGGCATTG CTACTCAAGA TCCAGTGCTG AGGGAAAAGT TTGCTGGAGA ACCTGAACAT 
GTTATCAACT TCTTTTTTAT GCTAGCAGAG GAGCTCAGGG AGATTATGGC TCAGCTTGGT 
TTCCGTACGA TGACTGAGAT GGTTGGTCGT TCAGACATGC TTGAAGTTGA TAAAGAAGTT 
GTTAAGAGCA ACGAGAAGCT GGAAAATATT GATCTCTCTT TGTTACTTAG ACCTGCTGCT 
GACATTCGAC CTGAAGCTGC TCAGTATTGT GTCCAAAAGC AGGATCATGG TTTGGACATG 
GCATTGGATA ATAAACTCAT TAAACTCTCT GAGGCTGCAT TGGAAAAAGG TCTTCCTGTG 
TACATTGAAA CACCTATCTG CAATGTGAAC CGTGCTGTTG GAACGATGCT TAGTCATGAA 
GTTACTAAGC GTTACCACTT GGCAGGGCTT CCCGCAGATA CTATTCACAT CAAACTCACA 
GGAAGTGCTG GGCAGAGTCT AGGAGCTTTC CTTTGCCCTG GCATTATGCT GGAACTGGAA 
GGGGATGGCA ATGACTACGT TGGTAAAGGA TTATCAGGTG GGAAGATTGT AGTTTATCCT 
CCAAAAGGAA GCCTGTTTGA TCCAAAAGAA AACATTGTGA TCGGTAATGT AGCTCTTTAT 
GGAGCCACAT GTGGTGAAGC ATATTTCAAT GGGATGGCAG CAGAAAGATT TTGTGTCCGT 
AATTCTGGGG CAAGGGCTGT TGTAGAAGGT GTTGGTGATC ATGGCTGTGA GTACATGACA 
GGTGGAACTG TTGTTGTGCT TGGGAAAACT GGAAGGAATT TTGCTGCTGG TATGAGTGGT 
GGTGTTGCTT ATGTTCTTGA TTTGGATGGG AAATTCAGAT CTCGTTGCAA TCCTGAACTC 
GTAGATCTTG ACAAAGTCGA GGAAGAGGAG GACATTACAA CTCTAAAAAT GATGATACAA 
CAACACCAGC GTCACACAAA TAGCCTGCTA GCTAGAGAAG TACTTGCTGA TTTTGATAAT 
CTTCTACCTA AATTTATTAA GGTCTTCCCG AGGGATTACA AACGTGTTCT TGCCAACATG 
AAAGAGGAAT CTGCCACAAA AGAGGCTGCT GACCTAGCTG CTAAAGAAGT TGAGGAAGCT 
GAGGAGCAAG ATGAAGCAGA GTTGAAGGAG AAAGATGCTT TTGAGGAGCT AAAGAAGCTG 
GCAGCTGCAT CTTTGAATGG GAATTCCATT CAGGTAGAAG ATGGACCTCT GAAAAGGCCT 
ACCCGTGTTA ATGATGCTGT TAAACATCGA GGGTTTATTG CTTATGAGCG AGAAGGTGTT 
CAGTACAGAG ATCCAAATAT TCGAATGAAT GACTGGAAGG AAGTTACTGA GGAGTCAAAA 
CCTGGCCCTC TTTTAAAGAC CCAGTCAGCC CGTTGCATGG ACTGTGGCAC TCCTTTCTGT 
CACCAGGAGA ACTCAGGATG CCCTCTTGGA AACAAAATCC CTGAATTTAA TGAGCTAGTG 
CATCAAAATC GGTGGCGTGA AGCATTAGAT AGGTTACTTG AGACAAATAA CTTCCCAGAG 
TTCACTGGCA GAGTGTGCCC TGCACCCTGT GAAGGTTCCT GTGTTCTTGG GATAATTGAT 
GATCCAGTAT CTATAAAAAA CATTGAATGT TCAATTATTG ACAAGGCCTT TGAGGAGGGT 
TGGATGGTTC CGCGGCCTCC CCTCAAGAGA ACTGGGAGAC GAGTGGCTAT TGTTGGAAGC 
GGGCCTTCTG GTTTGGCTGC TGCTGATCAA CTAAACAAAA TGGGTCATTT GGTGACTGTG 
TATGAGCGTG CTGATCGAAT TGGAGGGCTA ATGATGTATG GAGTTCCCAA CATGAAAACT 
GACAAAGTGG ATATAGTTCA ACGGCGTGTT AACCTTATGT CTGAGGAAGG CATCAATTTT 
GTGGTAAATG CTAATGTTGG AATTGATCCT TTGTATTCTC TTGATCGGCT TCGAGATGAG 
AACAATGCGA TTGTTTTGGC AGTGGGAGCC ACAAAACCAA GGCAATTGTC AGGCGTCCAT 
TTTGCTATGC AGTTTCTTCA TGCAAATACC AAAAGTTTGC TTGACAGCAA TTTGCAGGAT 
GGTAACTACA TATCCGCAAA TGGCAAGAAA GTTGTTGTCA TTGGTGGAGG TGACACTGGA 
ACAGATTGCA TTGGGACATC TATTCGCCAT GGGTGTAGTA GCATTGTAAA TCTAGAGCTT 
CTACCTGAGC CACCACGAAC TAGAGGTCCG GGCAACCCTT GGCCACAGTG GCCTAGAGTA 
TTCCGGGTAG ACTATGGGCA CCAGGAAGCT GCCGCCAAGT TCGGGAAAGA CCCGAGGTCT 
TATGAGGTGT TGACTAAGCG TTTCATTGGA GATGAGAATG GGAATGTGAA AGGACTTGAG 
CTGGTACGTG TCCATTGGGA GAAGGATGCC ACAGGGAAAT TTCAATTTAA GGAGGTTGAG 
GGCTCCGAGG AAGTTATTGA GGCTGACCTA GTCCTACTAG CAATGGGATT CCTTGGTCCT 
GAGCTGAATG TGGCAGAGAA GTTGGGCTTG GAGCAAGATA ATAGATCGAA TTTCAAGGCA 
GAATATGGCC GCTTCTCAAC CAATGTGGAA GGGATCTTTG CAGCTGGGGA TTGCAGGCGA 
GGCCAGTCCC TAGTTGTATG GGCAATATCA GAGGGACGAC AAGCTGCTTC CCAGGTGGAC 
AAATATCTCA TGAAAGAAGA GGATGCTACC ATTAACACCG ATAATACTCA GGATGACCTT 
GTCAAGAGGC ACCAGGACCT CACCAAGAGA CATCAAGACA GCAGCAAGCA CACTGTCATG 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002321436.2
CDS	69..6734
Translation	MSAAASSGALLQPKSVKAPFSSLSKSSSLSPSLNVATAASVSRRSARANRCASTRKSVVVERKSFLGSKVRGSAGSERLHFWQSDGPGREPKLRVVVRSALSGVPEKPLGLYDPSFDKDSCGVGFVAELSGDSSRKTVNDALEMLVRMTHRGACGCETNTGDGAGILVALPHDFYKEVAKDIGFELPPPGEYAVGMFFLPTSDNRKEESKNVFTKVAESLGHTVLGWRPVPTDNSGLGNSALQTEPVIEQVFLTATPRSKADFEQQMYILRRVSMVAIRAALNLQYGGVRDFYICSLSSRTVVYKGQLKPEQLKGYYYADLGNERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLIKCKELGLSKNEMKKLLPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNMDPQRRALYEYFSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTRSGRVIMASEVGVVDIPPEDVLRKGRLNPGMMLLVDFEKHIIVDDEALKQQYSLARPYGEWLKRQKIELSDIVDSVQESERVAPAISGVVPASDDDTSMQNMGTHGLLAPLKAFGYTVEALEMLMLPMAKDATEALGSMGNDAPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAMKKMNFSGWRSKVLDITYSKERGRKGLEETLDRICAEAHEAIKEGYTVLVLSDRAFSSKRVAASSLLAVGAVHQYLVKKLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPPKSTGEFHTKDELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEGLGLSSEVIDKCFAGTPSRVEGATFEMLARDSLHLHELAFPSRVLPPGSAEAVALPNPGDYHWRKGGEIHLNDPLAIAKLQEAARGNSVAAYKEYSKRVQELNKACNLRGLLKFKEADVKVSLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAQAMNKIGGKSNTGEGGEQPSRMETLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEELREIMAQLGFRTMTEMVGRSDMLEVDKEVVKSNEKLENIDLSLLLRPAADIRPEAAQYCVQKQDHGLDMALDNKLIKLSEAALEKGLPVYIETPICNVNRAVGTMLSHEVTKRYHLAGLPADTIHIKLTGSAGQSLGAFLCPGIMLELEGDGNDYVGKGLSGGKIVVYPPKGSLFDPKENIVIGNVALYGATCGEAYFNGMAAERFCVRNSGARAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGVAYVLDLDGKFRSRCNPELVDLDKVEEEEDITTLKMMIQQHQRHTNSLLAREVLADFDNLLPKFIKVFPRDYKRVLANMKEESATKEAADLAAKEVEEAEEQDEAELKEKDAFEELKKLAAASLNGNSIQVEDGPLKRPTRVNDAVKHRGFIAYEREGVQYRDPNIRMNDWKEVTEESKPGPLLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVHQNRWREALDRLLETNNFPEFTGRVCPAPCEGSCVLGIIDDPVSIKNIECSIIDKAFEEGWMVPRPPLKRTGRRVAIVGSGPSGLAAADQLNKMGHLVTVYERADRIGGLMMYGVPNMKTDKVDIVQRRVNLMSEEGINFVVNANVGIDPLYSLDRLRDENNAIVLAVGATKPRQLSGVHFAMQFLHANTKSLLDSNLQDGNYISANGKKVVVIGGGDTGTDCIGTSIRHGCSSIVNLELLPEPPRTRGPGNPWPQWPRVFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGNVKGLELVRVHWEKDATGKFQFKEVEGSEEVIEADLVLLAMGFLGPELNVAEKLGLEQDNRSNFKAEYGRFSTNVEGIFAAGDCRRGQSLVVWAISEGRQAASQVDKYLMKEEDATINTDNTQDDLVKRHQDLTKRHQDSSKHTVMT

Target ORF information:

RefSeq Version	XM_002321400.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa NADH-dependent glutamate synthase family protein (POPTR_0015s01950g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002321400.2

ORF Insert Sequence:

ATGTCGGCTG CAGCGAGCTC TGGAGCTCTC CTTCAACCAA AATCGGTGAA AGCTCCGTTT 
TCTTCGCTTA GTAAATCGTC TTCTCTTTCT CCTTCGCTCA ATGTTGCCAC AGCAGCATCT 
GTTTCTCGGC GGAGCGCGAG GGCAAACCGA TGTGCTTCGA CGAGGAAATC GGTGGTTGTT 
GAGAGAAAAT CGTTTTTGGG AAGTAAAGTG CGGGGGTCTG CTGGATCGGA GAGATTGCAT 
TTTTGGCAGT CGGATGGACC GGGTAGAGAG CCGAAACTTC GAGTTGTTGT CCGGTCTGCA 
TTGTCCGGTG TGCCGGAGAA GCCGCTTGGG TTGTATGACC CGTCGTTTGA TAAGGATTCG 
TGCGGGGTTG GTTTTGTGGC TGAATTATCT GGGGATAGCA GTCGTAAAAC GGTAAATGAT 
GCTTTGGAGA TGTTGGTGAG GATGACACAT AGAGGGGCTT GTGGATGTGA GACGAATACT 
GGTGATGGGG CTGGTATTCT TGTGGCTCTT CCTCATGATT TCTACAAGGA GGTTGCTAAG 
GATATAGGGT TCGAACTGCC ACCACCAGGG GAATATGCAG TTGGCATGTT CTTTTTGCCC 
ACATCTGATA ATCGTAAGGA AGAAAGCAAG AATGTATTCA CTAAGGTTGC GGAGTCTCTT 
GGGCATACTG TACTTGGGTG GCGACCAGTA CCAACAGATA ACTCTGGACT GGGAAATTCT 
GCTTTGCAGA CCGAGCCTGT CATTGAGCAA GTGTTCCTTA CAGCCACTCC TAGGTCGAAG 
GCTGATTTTG AGCAGCAGAT GTACATATTA AGGAGGGTGT CAATGGTAGC TATTAGAGCT 
GCACTAAACC TTCAATATGG TGGTGTTAGG GACTTTTATA TCTGTTCCCT TTCCTCAAGG 
ACGGTTGTTT ATAAAGGTCA GTTGAAGCCT GAACAGTTGA AGGGTTACTA TTATGCAGAT 
CTTGGCAATG AAAGGTTTAC GAGCTACATG GCCCTGGTAC ACTCACGATT TTCAACAAAT 
ACATTTCCTA GCTGGGATCG TGCACAACCC ATGCGTGTCT TGGGCCATAA TGGGGAAATC 
AACACGCTAA GAGGCAATGT AAACTGGATG AAGGCTCGCG AGGGCCTAAT AAAGTGCAAG 
GAGCTCGGTC TCTCGAAGAA TGAGATGAAG AAGCTTCTTC CAATTGTAGA TGCTAGTTCT 
TCCGACTCAG GGGCTTTTGA TGGTGTGCTG GAGCTTTTAA TTCGAGCTGG GAGAAGTCTC 
CCTGAAGCTG TCATGATGAT GATTCCTGAA GCCTGGCAAA ATGACAAGAA TATGGATCCT 
CAAAGGAGGG CACTGTACGA ATACTTCTCA GCTCTTATGG AACCGTGGGA TGGGCCTGCA 
CTTATCTCAT TTACTGATGG CCACTACTTG GGAGCTACTC TGGATCGCAA TGGGTTACGT 
CCAGGAAGAT TTTATGTTAC ACGAAGTGGA CGAGTTATCA TGGCTAGTGA AGTTGGTGTT 
GTGGATATTC CTCCTGAAGA TGTCCTTAGG AAAGGAAGAC TTAACCCTGG AATGATGCTT 
CTGGTGGATT TTGAGAAGCA TATTATTGTA GATGATGAAG CCTTGAAGCA ACAATATTCC 
CTAGCAAGGC CCTATGGAGA GTGGCTGAAG AGGCAAAAGA TTGAACTCAG TGACATAGTT 
GACTCGGTTC AAGAATCTGA GAGAGTTGCT CCAGCCATTT CTGGGGTTGT TCCTGCATCT 
GATGATGACA CCAGCATGCA GAATATGGGC ACTCATGGTT TGCTGGCACC ATTAAAAGCT 
TTTGGCTATA CTGTTGAAGC CTTGGAGATG CTGATGCTTC CCATGGCAAA GGACGCTACA 
GAGGCTCTTG GTTCGATGGG AAATGATGCT CCCTTGGCTG TCATGTCTAA CAGGGAAAAA 
CTCACTTTCG AGTACTTCAA GCAAATGTTT GCTCAAGTGA CAAACCCTCC AATTGATCCC 
ATCAGAGAGA AGATTGTCAC TTCCATGGAA TGCATGATTG GGCCAGAAGG TGATTTGACA 
GAAACAACTG AAGAACAATG CCACCGTCTC TCTCTAAAAG GCCCTCTTTT ATCAATTGAG 
CAAATGGAAG CAATGAAAAA AATGAACTTT AGCGGTTGGC GAAGCAAAGT TCTCGACATA 
ACTTATTCAA AGGAACGGGG TAGGAAGGGA TTGGAGGAGA CTTTAGATAG GATTTGTGCT 
GAAGCTCACG AGGCAATCAA GGAAGGTTAT ACTGTGTTGG TTCTTTCTGA CAGAGCCTTC 
TCATCAAAGC GTGTTGCTGC AAGCTCCCTC TTGGCTGTTG GCGCTGTCCA CCAATATCTA 
GTAAAAAAGC TTGAGCGAAC TCAAGTCGGG TTGATAGTTG AATCTGCTGA ACCACGTGAA 
GTGCACCATT TCTGTACGTT GGTTGGTTTT GGCGCAGATG CTATCTGCCC ATACTTAGCT 
GTAGAGGCCA TTTGGAGATT ACAGGTTGAT GGCAAGATCC CACCAAAATC CACTGGCGAG 
TTCCACACAA AGGATGAGCT AGTCAAGAAG TATTTCAAGG CAAGCAACTA TGGCATGATG 
AAAGTGCTTG CCAAAATGGG AATTTCAACT TTGGCCTCCT ACAAGGGTGC TCAGATTTTT 
GAAGGCCTGG GCCTTTCATC AGAAGTGATT GACAAGTGCT TTGCAGGAAC TCCAAGCAGG 
GTAGAAGGAG CAACATTTGA GATGCTTGCC CGTGATTCAC TTCATTTGCA TGAGTTGGCA 
TTTCCTTCCC GTGTTCTCCC TCCTGGAAGT GCAGAAGCTG TAGCACTGCC CAATCCGGGG 
GATTATCATT GGAGAAAAGG TGGTGAGATT CACCTTAATG ATCCTCTTGC TATTGCAAAG 
CTTCAAGAAG CTGCCAGAGG TAATAGTGTG GCTGCCTATA AGGAATATTC TAAACGTGTT 
CAGGAGTTAA ACAAAGCTTG TAATTTGCGT GGGCTTTTGA AGTTCAAAGA GGCAGATGTG 
AAAGTTTCTT TGGATGAAGT AGAACCAGCC AGTGAGATTG TTAAAAGGTT CTGTACTGGT 
GCAATGAGTT ATGGATCAAT ATCATTGGAG GCACACACCA CACTGGCTCA GGCTATGAAC 
AAAATTGGAG GAAAATCAAA CACAGGTGAG GGAGGTGAAC AACCATCTCG GATGGAGACT 
CTTCCTGATG GTTCAATGAA CCCAAAGAGG AGTGCAATTA AGCAGGTTGC AAGTGGGAGA 
TTTGGAGTGT CAAGTTACTA TCTTACAAAT GCTGATGAGC TGCAGATAAA GATGGCTCAG 
GGTGCCAAGC CTGGTGAAGG AGGTGAGCTT CCTGGCCACA AGGTCATAGG AGACATTGCA 
ATTACCAGAA ATTCTACTGC AGGGGTGGGC CTCATTAGTC CTCCTCCCCA TCATGATATC 
TATTCAATTG AAGACCTTGC TCAATTGATT CACGATCTCA AGAATGCCAA TCCAGCAGCC 
AGAATAAGTG TTAAGTTGGT ATCTGAAGCT GGTGTCGGAG TCATTGCCAG TGGGGTGGTA 
AAGGGACATG CTGACCATGT TTTGATCTCA GGTCATGATG GAGGTACAGG TGCTTCTCGA 
TGGACTGGCA TCAAGAACGC TGGGCTCCCA TGGGAACTTG GTTTGGCTGA GACCCATCAA 
ACACTTGTTG CCAATGACCT TCGTGGCCGA ACAGTTCTTC AGACTGATGG CCAACTAAAA 
ACTGGACGAG ATGTGGCCAT TGCAGCCCTT CTTGGCGCAG AAGAGTTTGG TTTCAGCACT 
GCACCCCTTA TAACACTTGG TTGCATCATG ATGCGAAAGT GCCATAAGAA CACCTGCCCC 
GTAGGCATTG CTACTCAAGA TCCAGTGCTG AGGGAAAAGT TTGCTGGAGA ACCTGAACAT 
GTTATCAACT TCTTTTTTAT GCTAGCAGAG GAGCTCAGGG AGATTATGGC TCAGCTTGGT 
TTCCGTACGA TGACTGAGAT GGTTGGTCGT TCAGACATGC TTGAAGTTGA TAAAGAAGTT 
GTTAAGAGCA ACGAGAAGCT GGAAAATATT GATCTCTCTT TGTTACTTAG ACCTGCTGCT 
GACATTCGAC CTGAAGCTGC TCAGTATTGT GTCCAAAAGC AGGATCATGG TTTGGACATG 
GCATTGGATA ATAAACTCAT TAAACTCTCT GAGGCTGCAT TGGAAAAAGG TCTTCCTGTG 
TACATTGAAA CACCTATCTG CAATGTGAAC CGTGCTGTTG GAACGATGCT TAGTCATGAA 
GTTACTAAGC GTTACCACTT GGCAGGGCTT CCCGCAGATA CTATTCACAT CAAACTCACA 
GGAAGTGCTG GGCAGAGTCT AGGAGCTTTC CTTTGCCCTG GCATTATGCT GGAACTGGAA 
GGGGATGGCA ATGACTACGT TGGTAAAGGA TTATCAGGTG GGAAGATTGT AGTTTATCCT 
CCAAAAGGAA GCCTGTTTGA TCCAAAAGAA AACATTGTGA TCGGTAATGT AGCTCTTTAT 
GGAGCCACAT GTGGTGAAGC ATATTTCAAT GGGATGGCAG CAGAAAGATT TTGTGTCCGT 
AATTCTGGGG CAAGGGCTGT TGTAGAAGGT GTTGGTGATC ATGGCTGTGA GTACATGACA 
GGTGGAACTG TTGTTGTGCT TGGGAAAACT GGAAGGAATT TTGCTGCTGG TATGAGTGGT 
GGTGTTGCTT ATGTTCTTGA TTTGGATGGG AAATTCAGAT CTCGTTGCAA TCCTGAACTC 
GTAGATCTTG ACAAAGTCGA GGAAGAGGAG GACATTACAA CTCTAAAAAT GATGATACAA 
CAACACCAGC GTCACACAAA TAGCCTGCTA GCTAGAGAAG TACTTGCTGA TTTTGATAAT 
CTTCTACCTA AATTTATTAA GGTCTTCCCG AGGGATTACA AACGTGTTCT TGCCAACATG 
AAAGAGGAAT CTGCCACAAA AGAGGCTGCT GACCTAGCTG CTAAAGAAGT TGAGGAAGCT 
GAGGAGCAAG ATGAAGCAGA GTTGAAGGAG AAAGATGCTT TTGAGGAGCT AAAGAAGCTG 
GCAGCTGCAT CTTTGAATGG GAATTCCATT CAGGTAGAAG ATGGACCTCT GAAAAGGCCT 
ACCCGTGTTA ATGATGCTGT TAAACATCGA GGGTTTATTG CTTATGAGCG AGAAGGTGTT 
CAGTACAGAG ATCCAAATAT TCGAATGAAT GACTGGAAGG AAGTTACTGA GGAGTCAAAA 
CCTGGCCCTC TTTTAAAGAC CCAGTCAGCC CGTTGCATGG ACTGTGGCAC TCCTTTCTGT 
CACCAGGAGA ACTCAGGATG CCCTCTTGGA AACAAAATCC CTGAATTTAA TGAGCTAGTG 
CATCAAAATC GGTGGCGTGA AGCATTAGAT AGGTTACTTG AGACAAATAA CTTCCCAGAG 
TTCACTGGCA GAGTGTGCCC TGCACCCTGT GAAGGTTCCT GTGTTCTTGG GATAATTGAT 
GATCCAGTAT CTATAAAAAA CATTGAATGT TCAATTATTG ACAAGGCCTT TGAGGAGGGT 
TGGATGGTTC CGCGGCCTCC CCTCAAGAGA ACTGGGAGAC GAGTGGCTAT TGTTGGAAGC 
GGGCCTTCTG GTTTGGCTGC TGCTGATCAA CTAAACAAAA TGGGTCATTT GGTGACTGTG 
TATGAGCGTG CTGATCGAAT TGGAGGGCTA ATGATGTATG GAGTTCCCAA CATGAAAACT 
GACAAAGTGG ATATAGTTCA ACGGCGTGTT AACCTTATGT CTGAGGAAGG CATCAATTTT 
GTGGTAAATG CTAATGTTGG AATTGATCCT TTGTATTCTC TTGATCGGCT TCGAGATGAG 
AACAATGCGA TTGTTTTGGC AGTGGGAGCC ACAAAACCAA GGCAATTGTC AGGCGTCCAT 
TTTGCTATGC AGTTTCTTCA TGCAAATACC AAAAGTTTGC TTGACAGCAA TTTGCAGGAT 
GGTAACTACA TATCCGCAAA TGGCAAGAAA GTTGTTGTCA TTGGTGGAGG TGACACTGGA 
ACAGATTGCA TTGGGACATC TATTCGCCAT GGGTGTAGTA GCATTGTAAA TCTAGAGCTT 
CTACCTGAGC CACCACGAAC TAGAGGTCCG GGCAACCCTT GGCCACAGTG GCCTAGAGTA 
TTCCGGGTAG ACTATGGGCA CCAGGAAGCT GCCGCCAAGT TCGGGAAAGA CCCGAGGTCT 
TATGAGGTGT TGACTAAGCG TTTCATTGGA GATGAGAATG GGAATGTGAA AGGACTTGAG 
CTGGTACGTG TCCATTGGGA GAAGGATGCC ACAGGGAAAT TTCAATTTAA GGAGGTTGAG 
GGCTCCGAGG AAGTTATTGA GGCTGACCTA GTCCTACTAG CAATGGGATT CCTTGGTCCT 
GAGCTGAATG TGGCAGAGAA GTTGGGCTTG GAGCAAGATA ATAGATCGAA TTTCAAGGCA 
GAATATGGCC GCTTCTCAAC CAATGTGGAA GGGATCTTTG CAGCTGGGGA TTGCAGGCGA 
GGCCAGTCCC TAGTTGTATG GGCAATATCA GAGGGACGAC AAGCTGCTTC CCAGGTGGAC 
AAATATCTCA TGAAAGAAGA GGATGCTACC ATTAACACCG ATAATACTCA GGATGACCTT 
GTCAAGAGGC ACCAGGACCT CACCAAGAGA CATCAAGACA GCAGCAAGCA CACTGTCATG 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7453835 ( XM_002321983.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002322019.2 Populus trichocarpa hypothetical protein (POPTR_0015s01950g) mRNA, complete cds.

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CloneID	OPa31061
Clone ID Related Accession (Same CDS sequence)	XM_002321983.2
Accession Version	XM_002321983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1388332800000
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008481). On Dec 16, 2013 this sequence version replaced XM_002321983.1.

ATGTCGGCTG CAGCGAGCTC TGGAGCTCTC CTTCAACCAA AATCGGTGAA AGCTCCGTTT 
TCTTCGCTTA GTAAATCGTC TTCTCTTTCT CCTTCGCTCA ATGTTGCCAC AGCAGCATCT 
GTTTCTCGGC GGAGCGCGAG GGCAAACCGA TGTGCTTCGA CGAGGAAATC GGTGGTTGTT 
GAGAGAAAAT CGTTTTTGGG AAGTAAAGTG CGGGGGTCTG CTGGATCGGA GAGATTGCAT 
TTTTGGCAGT CGGATGGACC GGGTAGAGAG CCGAAACTTC GAGTTGTTGT CCGGTCTGCA 
TTGTCCGGTG TGCCGGAGAA GCCGCTTGGG TTGTATGACC CGTCGTTTGA TAAGGATTCG 
TGCGGGGTTG GTTTTGTGGC TGAATTATCT GGGGATAGCA GTCGTAAAAC GGTAAATGAT 
GCTTTGGAGA TGTTGGTGAG GATGACACAT AGAGGGGCTT GTGGATGTGA GACGAATACT 
GGTGATGGGG CTGGTATTCT TGTGGCTCTT CCTCATGATT TCTACAAGGA GGTTGCTAAG 
GATATAGGGT TCGAACTGCC ACCACCAGGG GAATATGCAG TTGGCATGTT CTTTTTGCCC 
ACATCTGATA ATCGTAAGGA AGAAAGCAAG AATGTATTCA CTAAGGTTGC GGAGTCTCTT 
GGGCATACTG TACTTGGGTG GCGACCAGTA CCAACAGATA ACTCTGGACT GGGAAATTCT 
GCTTTGCAGA CCGAGCCTGT CATTGAGCAA GTGTTCCTTA CAGCCACTCC TAGGTCGAAG 
GCTGATTTTG AGCAGCAGAT GTACATATTA AGGAGGGTGT CAATGGTAGC TATTAGAGCT 
GCACTAAACC TTCAATATGG TGGTGTTAGG GACTTTTATA TCTGTTCCCT TTCCTCAAGG 
ACGGTTGTTT ATAAAGGTCA GTTGAAGCCT GAACAGTTGA AGGGTTACTA TTATGCAGAT 
CTTGGCAATG AAAGGTTTAC GAGCTACATG GCCCTGGTAC ACTCACGATT TTCAACAAAT 
ACATTTCCTA GCTGGGATCG TGCACAACCC ATGCGTGTCT TGGGCCATAA TGGGGAAATC 
AACACGCTAA GAGGCAATGT AAACTGGATG AAGGCTCGCG AGGGCCTAAT AAAGTGCAAG 
GAGCTCGGTC TCTCGAAGAA TGAGATGAAG AAGCTTCTTC CAATTGTAGA TGCTAGTTCT 
TCCGACTCAG GGGCTTTTGA TGGTGTGCTG GAGCTTTTAA TTCGAGCTGG GAGAAGTCTC 
CCTGAAGCTG TCATGATGAT GATTCCTGAA GCCTGGCAAA ATGACAAGAA TATGGATCCT 
CAAAGGAGGG CACTGTACGA ATACTTCTCA GCTCTTATGG AACCGTGGGA TGGGCCTGCA 
CTTATCTCAT TTACTGATGG CCACTACTTG GGAGCTACTC TGGATCGCAA TGGGTTACGT 
CCAGGAAGAT TTTATGTTAC ACGAAGTGGA CGAGTTATCA TGGCTAGTGA AGTTGGTGTT 
GTGGATATTC CTCCTGAAGA TGTCCTTAGG AAAGGAAGAC TTAACCCTGG AATGATGCTT 
CTGGTGGATT TTGAGAAGCA TATTATTGTA GATGATGAAG CCTTGAAGCA ACAATATTCC 
CTAGCAAGGC CCTATGGAGA GTGGCTGAAG AGGCAAAAGA TTGAACTCAG TGACATAGTT 
GACTCGGTTC AAGAATCTGA GAGAGTTGCT CCAGCCATTT CTGGGGTTGT TCCTGCATCT 
GATGATGACA CCAGCATGCA GAATATGGGC ACTCATGGTT TGCTGGCACC ATTAAAAGCT 
TTTGGCTATA CTGTTGAAGC CTTGGAGATG CTGATGCTTC CCATGGCAAA GGACGCTACA 
GAGGCTCTTG GTTCGATGGG AAATGATGCT CCCTTGGCTG TCATGTCTAA CAGGGAAAAA 
CTCACTTTCG AGTACTTCAA GCAAATGTTT GCTCAAGTGA CAAACCCTCC AATTGATCCC 
ATCAGAGAGA AGATTGTCAC TTCCATGGAA TGCATGATTG GGCCAGAAGG TGATTTGACA 
GAAACAACTG AAGAACAATG CCACCGTCTC TCTCTAAAAG GCCCTCTTTT ATCAATTGAG 
CAAATGGAAG CAATGAAAAA AATGAACTTT AGCGGTTGGC GAAGCAAAGT TCTCGACATA 
ACTTATTCAA AGGAACGGGG TAGGAAGGGA TTGGAGGAGA CTTTAGATAG GATTTGTGCT 
GAAGCTCACG AGGCAATCAA GGAAGGTTAT ACTGTGTTGG TTCTTTCTGA CAGAGCCTTC 
TCATCAAAGC GTGTTGCTGC AAGCTCCCTC TTGGCTGTTG GCGCTGTCCA CCAATATCTA 
GTAAAAAAGC TTGAGCGAAC TCAAGTCGGG TTGATAGTTG AATCTGCTGA ACCACGTGAA 
GTGCACCATT TCTGTACGTT GGTTGGTTTT GGCGCAGATG CTATCTGCCC ATACTTAGCT 
GTAGAGGCCA TTTGGAGATT ACAGGTTGAT GGCAAGATCC CACCAAAATC CACTGGCGAG 
TTCCACACAA AGGATGAGCT AGTCAAGAAG TATTTCAAGG CAAGCAACTA TGGCATGATG 
AAAGTGCTTG CCAAAATGGG AATTTCAACT TTGGCCTCCT ACAAGGGTGC TCAGATTTTT 
GAAGGCCTGG GCCTTTCATC AGAAGTGATT GACAAGTGCT TTGCAGGAAC TCCAAGCAGG 
GTAGAAGGAG CAACATTTGA GATGCTTGCC CGTGATTCAC TTCATTTGCA TGAGTTGGCA 
TTTCCTTCCC GTGTTCTCCC TCCTGGAAGT GCAGAAGCTG TAGCACTGCC CAATCCGGGG 
GATTATCATT GGAGAAAAGG TGGTGAGATT CACCTTAATG ATCCTCTTGC TATTGCAAAG 
CTTCAAGAAG CTGCCAGAGG TAATAGTGTG GCTGCCTATA AGGAATATTC TAAACGTGTT 
CAGGAGTTAA ACAAAGCTTG TAATTTGCGT GGGCTTTTGA AGTTCAAAGA GGCAGATGTG 
AAAGTTTCTT TGGATGAAGT AGAACCAGCC AGTGAGATTG TTAAAAGGTT CTGTACTGGT 
GCAATGAGTT ATGGATCAAT ATCATTGGAG GCACACACCA CACTGGCTCA GGCTATGAAC 
AAAATTGGAG GAAAATCAAA CACAGGTGAG GGAGGTGAAC AACCATCTCG GATGGAGACT 
CTTCCTGATG GTTCAATGAA CCCAAAGAGG AGTGCAATTA AGCAGGTTGC AAGTGGGAGA 
TTTGGAGTGT CAAGTTACTA TCTTACAAAT GCTGATGAGC TGCAGATAAA GATGGCTCAG 
GGTGCCAAGC CTGGTGAAGG AGGTGAGCTT CCTGGCCACA AGGTCATAGG AGACATTGCA 
ATTACCAGAA ATTCTACTGC AGGGGTGGGC CTCATTAGTC CTCCTCCCCA TCATGATATC 
TATTCAATTG AAGACCTTGC TCAATTGATT CACGATCTCA AGAATGCCAA TCCAGCAGCC 
AGAATAAGTG TTAAGTTGGT ATCTGAAGCT GGTGTCGGAG TCATTGCCAG TGGGGTGGTA 
AAGGGACATG CTGACCATGT TTTGATCTCA GGTCATGATG GAGGTACAGG TGCTTCTCGA 
TGGACTGGCA TCAAGAACGC TGGGCTCCCA TGGGAACTTG GTTTGGCTGA GACCCATCAA 
ACACTTGTTG CCAATGACCT TCGTGGCCGA ACAGTTCTTC AGACTGATGG CCAACTAAAA 
ACTGGACGAG ATGTGGCCAT TGCAGCCCTT CTTGGCGCAG AAGAGTTTGG TTTCAGCACT 
GCACCCCTTA TAACACTTGG TTGCATCATG ATGCGAAAGT GCCATAAGAA CACCTGCCCC 
GTAGGCATTG CTACTCAAGA TCCAGTGCTG AGGGAAAAGT TTGCTGGAGA ACCTGAACAT 
GTTATCAACT TCTTTTTTAT GCTAGCAGAG GAGCTCAGGG AGATTATGGC TCAGCTTGGT 
TTCCGTACGA TGACTGAGAT GGTTGGTCGT TCAGACATGC TTGAAGTTGA TAAAGAAGTT 
GTTAAGAGCA ACGAGAAGCT GGAAAATATT GATCTCTCTT TGTTACTTAG ACCTGCTGCT 
GACATTCGAC CTGAAGCTGC TCAGTATTGT GTCCAAAAGC AGGATCATGG TTTGGACATG 
GCATTGGATA ATAAACTCAT TAAACTCTCT GAGGCTGCAT TGGAAAAAGG TCTTCCTGTG 
TACATTGAAA CACCTATCTG CAATGTGAAC CGTGCTGTTG GAACGATGCT TAGTCATGAA 
GTTACTAAGC GTTACCACTT GGCAGGGCTT CCCGCAGATA CTATTCACAT CAAACTCACA 
GGAAGTGCTG GGCAGAGTCT AGGAGCTTTC CTTTGCCCTG GCATTATGCT GGAACTGGAA 
GGGGATGGCA ATGACTACGT TGGTAAAGGA TTATCAGGTG GGAAGATTGT AGTTTATCCT 
CCAAAAGGAA GCCTGTTTGA TCCAAAAGAA AACATTGTGA TCGGTAATGT AGCTCTTTAT 
GGAGCCACAT GTGGTGAAGC ATATTTCAAT GGGATGGCAG CAGAAAGATT TTGTGTCCGT 
AATTCTGGGG CAAGGGCTGT TGTAGAAGGT GTTGGTGATC ATGGCTGTGA GTACATGACA 
GGTGGAACTG TTGTTGTGCT TGGGAAAACT GGAAGGAATT TTGCTGCTGG TATGAGTGGT 
GGTGTTGCTT ATGTTCTTGA TTTGGATGGG AAATTCAGAT CTCGTTGCAA TCCTGAACTC 
GTAGATCTTG ACAAAGTCGA GGAAGAGGAG GACATTACAA CTCTAAAAAT GATGATACAA 
CAACACCAGC GTCACACAAA TAGCCTGCTA GCTAGAGAAG TACTTGCTGA TTTTGATAAT 
CTTCTACCTA AATTTATTAA GGTCTTCCCG AGGGATTACA AACGTGTTCT TGCCAACATG 
AAAGAGGAAT CTGCCACAAA AGAGGCTGCT GACCTAGCTG CTAAAGAAGT TGAGGAAGCT 
GAGGAGCAAG ATGAAGCAGA GTTGAAGGAG AAAGATGCTT TTGAGGAGCT AAAGAAGCTG 
GCAGCTGCAT CTTTGAATGG GAATTCCATT CAGGTAGAAG ATGGACCTCT GAAAAGGCCT 
ACCCGTGTTA ATGATGCTGT TAAACATCGA GGGTTTATTG CTTATGAGCG AGAAGGTGTT 
CAGTACAGAG ATCCAAATAT TCGAATGAAT GACTGGAAGG AAGTTACTGA GGAGTCAAAA 
CCTGGCCCTC TTTTAAAGAC CCAGTCAGCC CGTTGCATGG ACTGTGGCAC TCCTTTCTGT 
CACCAGGAGA ACTCAGGATG CCCTCTTGGA AACAAAATCC CTGAATTTAA TGAGCTAGTG 
CATCAAAATC GGTGGCGTGA AGCATTAGAT AGGTTACTTG AGACAAATAA CTTCCCAGAG 
TTCACTGGCA GAGTGTGCCC TGCACCCTGT GAAGGTTCCT GTGTTCTTGG GATAATTGAT 
GATCCAGTAT CTATAAAAAA CATTGAATGT TCAATTATTG ACAAGGCCTT TGAGGAGGGT 
TGGATGGTTC CGCGGCCTCC CCTCAAGAGA ACTGGGAGAC GAGTGGCTAT TGTTGGAAGC 
GGGCCTTCTG GTTTGGCTGC TGCTGATCAA CTAAACAAAA TGGGTCATTT GGTGACTGTG 
TATGAGCGTG CTGATCGAAT TGGAGGGCTA ATGATGTATG GAGTTCCCAA CATGAAAACT 
GACAAAGTGG ATATAGTTCA ACGGCGTGTT AACCTTATGT CTGAGGAAGG CATCAATTTT 
GTGGTAAATG CTAATGTTGG AATTGATCCT TTGTATTCTC TTGATCGGCT TCGAGATGAG 
AACAATGCGA TTGTTTTGGC AGTGGGAGCC ACAAAACCAA GGGACCTTCC GGTACCTGGT 
CGGGAGTTGT CAGGCGTCCA TTTTGCTATG CAGTTTCTTC ATGCAAATAC CAAAAGTTTG 
CTTGACAGCA ATTTGCAGGA TGGTAACTAC ATATCCGCAA ATGGCAAGAA AGTTGTTGTC 
ATTGGTGGAG GTGACACTGG AACAGATTGC ATTGGGACAT CTATTCGCCA TGGGTGTAGT 
AGCATTGTAA ATCTAGAGCT TCTACCTGAG CCACCACGAA CTAGAGGTCC GGGCAACCCT 
TGGCCACAGT GGCCTAGAGT ATTCCGGGTA GACTATGGGC ACCAGGAAGC TGCCGCCAAG 
TTCGGGAAAG ACCCGAGGTC TTATGAGGTG TTGACTAAGC GTTTCATTGG AGATGAGAAT 
GGGAATGTGA AAGGACTTGA GCTGGTACGT GTCCATTGGG AGAAGGATGC CACAGGGAAA 
TTTCAATTTA AGGAGGTTGA GGGCTCCGAG GAAGTTATTG AGGCTGACCT AGTCCTACTA 
GCAATGGGAT TCCTTGGTCC TGAGCTGAAT GTGGCAGAGA AGTTGGGCTT GGAGCAAGAT 
AATAGATCGA ATTTCAAGGC AGAATATGGC CGCTTCTCAA CCAATGTGGA AGGGATCTTT 
GCAGCTGGGG ATTGCAGGCG AGGCCAGTCC CTAGTTGTAT GGGCAATATC AGAGGGACGA 
CAAGCTGCTT CCCAGGTGGA CAAATATCTC ATGAAAGAAG AGGATGCTAC CATTAACACC 
GATAATACTC AGGATGACCT TGTCAAGAGG CACCAGGACC TCACCAAGAG ACATCAAGAC 
AGCAGCAAGC ACACTGTCAT GACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002322019.2
CDS	69..6755
Translation	MSAAASSGALLQPKSVKAPFSSLSKSSSLSPSLNVATAASVSRRSARANRCASTRKSVVVERKSFLGSKVRGSAGSERLHFWQSDGPGREPKLRVVVRSALSGVPEKPLGLYDPSFDKDSCGVGFVAELSGDSSRKTVNDALEMLVRMTHRGACGCETNTGDGAGILVALPHDFYKEVAKDIGFELPPPGEYAVGMFFLPTSDNRKEESKNVFTKVAESLGHTVLGWRPVPTDNSGLGNSALQTEPVIEQVFLTATPRSKADFEQQMYILRRVSMVAIRAALNLQYGGVRDFYICSLSSRTVVYKGQLKPEQLKGYYYADLGNERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLIKCKELGLSKNEMKKLLPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNMDPQRRALYEYFSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTRSGRVIMASEVGVVDIPPEDVLRKGRLNPGMMLLVDFEKHIIVDDEALKQQYSLARPYGEWLKRQKIELSDIVDSVQESERVAPAISGVVPASDDDTSMQNMGTHGLLAPLKAFGYTVEALEMLMLPMAKDATEALGSMGNDAPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAMKKMNFSGWRSKVLDITYSKERGRKGLEETLDRICAEAHEAIKEGYTVLVLSDRAFSSKRVAASSLLAVGAVHQYLVKKLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPPKSTGEFHTKDELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEGLGLSSEVIDKCFAGTPSRVEGATFEMLARDSLHLHELAFPSRVLPPGSAEAVALPNPGDYHWRKGGEIHLNDPLAIAKLQEAARGNSVAAYKEYSKRVQELNKACNLRGLLKFKEADVKVSLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAQAMNKIGGKSNTGEGGEQPSRMETLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEELREIMAQLGFRTMTEMVGRSDMLEVDKEVVKSNEKLENIDLSLLLRPAADIRPEAAQYCVQKQDHGLDMALDNKLIKLSEAALEKGLPVYIETPICNVNRAVGTMLSHEVTKRYHLAGLPADTIHIKLTGSAGQSLGAFLCPGIMLELEGDGNDYVGKGLSGGKIVVYPPKGSLFDPKENIVIGNVALYGATCGEAYFNGMAAERFCVRNSGARAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGVAYVLDLDGKFRSRCNPELVDLDKVEEEEDITTLKMMIQQHQRHTNSLLAREVLADFDNLLPKFIKVFPRDYKRVLANMKEESATKEAADLAAKEVEEAEEQDEAELKEKDAFEELKKLAAASLNGNSIQVEDGPLKRPTRVNDAVKHRGFIAYEREGVQYRDPNIRMNDWKEVTEESKPGPLLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVHQNRWREALDRLLETNNFPEFTGRVCPAPCEGSCVLGIIDDPVSIKNIECSIIDKAFEEGWMVPRPPLKRTGRRVAIVGSGPSGLAAADQLNKMGHLVTVYERADRIGGLMMYGVPNMKTDKVDIVQRRVNLMSEEGINFVVNANVGIDPLYSLDRLRDENNAIVLAVGATKPRDLPVPGRELSGVHFAMQFLHANTKSLLDSNLQDGNYISANGKKVVVIGGGDTGTDCIGTSIRHGCSSIVNLELLPEPPRTRGPGNPWPQWPRVFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGNVKGLELVRVHWEKDATGKFQFKEVEGSEEVIEADLVLLAMGFLGPELNVAEKLGLEQDNRSNFKAEYGRFSTNVEGIFAAGDCRRGQSLVVWAISEGRQAASQVDKYLMKEEDATINTDNTQDDLVKRHQDLTKRHQDSSKHTVMT

Target ORF information:

RefSeq Version	XM_002321983.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0015s01950g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002321983.2

ORF Insert Sequence:

ATGTCGGCTG CAGCGAGCTC TGGAGCTCTC CTTCAACCAA AATCGGTGAA AGCTCCGTTT 
TCTTCGCTTA GTAAATCGTC TTCTCTTTCT CCTTCGCTCA ATGTTGCCAC AGCAGCATCT 
GTTTCTCGGC GGAGCGCGAG GGCAAACCGA TGTGCTTCGA CGAGGAAATC GGTGGTTGTT 
GAGAGAAAAT CGTTTTTGGG AAGTAAAGTG CGGGGGTCTG CTGGATCGGA GAGATTGCAT 
TTTTGGCAGT CGGATGGACC GGGTAGAGAG CCGAAACTTC GAGTTGTTGT CCGGTCTGCA 
TTGTCCGGTG TGCCGGAGAA GCCGCTTGGG TTGTATGACC CGTCGTTTGA TAAGGATTCG 
TGCGGGGTTG GTTTTGTGGC TGAATTATCT GGGGATAGCA GTCGTAAAAC GGTAAATGAT 
GCTTTGGAGA TGTTGGTGAG GATGACACAT AGAGGGGCTT GTGGATGTGA GACGAATACT 
GGTGATGGGG CTGGTATTCT TGTGGCTCTT CCTCATGATT TCTACAAGGA GGTTGCTAAG 
GATATAGGGT TCGAACTGCC ACCACCAGGG GAATATGCAG TTGGCATGTT CTTTTTGCCC 
ACATCTGATA ATCGTAAGGA AGAAAGCAAG AATGTATTCA CTAAGGTTGC GGAGTCTCTT 
GGGCATACTG TACTTGGGTG GCGACCAGTA CCAACAGATA ACTCTGGACT GGGAAATTCT 
GCTTTGCAGA CCGAGCCTGT CATTGAGCAA GTGTTCCTTA CAGCCACTCC TAGGTCGAAG 
GCTGATTTTG AGCAGCAGAT GTACATATTA AGGAGGGTGT CAATGGTAGC TATTAGAGCT 
GCACTAAACC TTCAATATGG TGGTGTTAGG GACTTTTATA TCTGTTCCCT TTCCTCAAGG 
ACGGTTGTTT ATAAAGGTCA GTTGAAGCCT GAACAGTTGA AGGGTTACTA TTATGCAGAT 
CTTGGCAATG AAAGGTTTAC GAGCTACATG GCCCTGGTAC ACTCACGATT TTCAACAAAT 
ACATTTCCTA GCTGGGATCG TGCACAACCC ATGCGTGTCT TGGGCCATAA TGGGGAAATC 
AACACGCTAA GAGGCAATGT AAACTGGATG AAGGCTCGCG AGGGCCTAAT AAAGTGCAAG 
GAGCTCGGTC TCTCGAAGAA TGAGATGAAG AAGCTTCTTC CAATTGTAGA TGCTAGTTCT 
TCCGACTCAG GGGCTTTTGA TGGTGTGCTG GAGCTTTTAA TTCGAGCTGG GAGAAGTCTC 
CCTGAAGCTG TCATGATGAT GATTCCTGAA GCCTGGCAAA ATGACAAGAA TATGGATCCT 
CAAAGGAGGG CACTGTACGA ATACTTCTCA GCTCTTATGG AACCGTGGGA TGGGCCTGCA 
CTTATCTCAT TTACTGATGG CCACTACTTG GGAGCTACTC TGGATCGCAA TGGGTTACGT 
CCAGGAAGAT TTTATGTTAC ACGAAGTGGA CGAGTTATCA TGGCTAGTGA AGTTGGTGTT 
GTGGATATTC CTCCTGAAGA TGTCCTTAGG AAAGGAAGAC TTAACCCTGG AATGATGCTT 
CTGGTGGATT TTGAGAAGCA TATTATTGTA GATGATGAAG CCTTGAAGCA ACAATATTCC 
CTAGCAAGGC CCTATGGAGA GTGGCTGAAG AGGCAAAAGA TTGAACTCAG TGACATAGTT 
GACTCGGTTC AAGAATCTGA GAGAGTTGCT CCAGCCATTT CTGGGGTTGT TCCTGCATCT 
GATGATGACA CCAGCATGCA GAATATGGGC ACTCATGGTT TGCTGGCACC ATTAAAAGCT 
TTTGGCTATA CTGTTGAAGC CTTGGAGATG CTGATGCTTC CCATGGCAAA GGACGCTACA 
GAGGCTCTTG GTTCGATGGG AAATGATGCT CCCTTGGCTG TCATGTCTAA CAGGGAAAAA 
CTCACTTTCG AGTACTTCAA GCAAATGTTT GCTCAAGTGA CAAACCCTCC AATTGATCCC 
ATCAGAGAGA AGATTGTCAC TTCCATGGAA TGCATGATTG GGCCAGAAGG TGATTTGACA 
GAAACAACTG AAGAACAATG CCACCGTCTC TCTCTAAAAG GCCCTCTTTT ATCAATTGAG 
CAAATGGAAG CAATGAAAAA AATGAACTTT AGCGGTTGGC GAAGCAAAGT TCTCGACATA 
ACTTATTCAA AGGAACGGGG TAGGAAGGGA TTGGAGGAGA CTTTAGATAG GATTTGTGCT 
GAAGCTCACG AGGCAATCAA GGAAGGTTAT ACTGTGTTGG TTCTTTCTGA CAGAGCCTTC 
TCATCAAAGC GTGTTGCTGC AAGCTCCCTC TTGGCTGTTG GCGCTGTCCA CCAATATCTA 
GTAAAAAAGC TTGAGCGAAC TCAAGTCGGG TTGATAGTTG AATCTGCTGA ACCACGTGAA 
GTGCACCATT TCTGTACGTT GGTTGGTTTT GGCGCAGATG CTATCTGCCC ATACTTAGCT 
GTAGAGGCCA TTTGGAGATT ACAGGTTGAT GGCAAGATCC CACCAAAATC CACTGGCGAG 
TTCCACACAA AGGATGAGCT AGTCAAGAAG TATTTCAAGG CAAGCAACTA TGGCATGATG 
AAAGTGCTTG CCAAAATGGG AATTTCAACT TTGGCCTCCT ACAAGGGTGC TCAGATTTTT 
GAAGGCCTGG GCCTTTCATC AGAAGTGATT GACAAGTGCT TTGCAGGAAC TCCAAGCAGG 
GTAGAAGGAG CAACATTTGA GATGCTTGCC CGTGATTCAC TTCATTTGCA TGAGTTGGCA 
TTTCCTTCCC GTGTTCTCCC TCCTGGAAGT GCAGAAGCTG TAGCACTGCC CAATCCGGGG 
GATTATCATT GGAGAAAAGG TGGTGAGATT CACCTTAATG ATCCTCTTGC TATTGCAAAG 
CTTCAAGAAG CTGCCAGAGG TAATAGTGTG GCTGCCTATA AGGAATATTC TAAACGTGTT 
CAGGAGTTAA ACAAAGCTTG TAATTTGCGT GGGCTTTTGA AGTTCAAAGA GGCAGATGTG 
AAAGTTTCTT TGGATGAAGT AGAACCAGCC AGTGAGATTG TTAAAAGGTT CTGTACTGGT 
GCAATGAGTT ATGGATCAAT ATCATTGGAG GCACACACCA CACTGGCTCA GGCTATGAAC 
AAAATTGGAG GAAAATCAAA CACAGGTGAG GGAGGTGAAC AACCATCTCG GATGGAGACT 
CTTCCTGATG GTTCAATGAA CCCAAAGAGG AGTGCAATTA AGCAGGTTGC AAGTGGGAGA 
TTTGGAGTGT CAAGTTACTA TCTTACAAAT GCTGATGAGC TGCAGATAAA GATGGCTCAG 
GGTGCCAAGC CTGGTGAAGG AGGTGAGCTT CCTGGCCACA AGGTCATAGG AGACATTGCA 
ATTACCAGAA ATTCTACTGC AGGGGTGGGC CTCATTAGTC CTCCTCCCCA TCATGATATC 
TATTCAATTG AAGACCTTGC TCAATTGATT CACGATCTCA AGAATGCCAA TCCAGCAGCC 
AGAATAAGTG TTAAGTTGGT ATCTGAAGCT GGTGTCGGAG TCATTGCCAG TGGGGTGGTA 
AAGGGACATG CTGACCATGT TTTGATCTCA GGTCATGATG GAGGTACAGG TGCTTCTCGA 
TGGACTGGCA TCAAGAACGC TGGGCTCCCA TGGGAACTTG GTTTGGCTGA GACCCATCAA 
ACACTTGTTG CCAATGACCT TCGTGGCCGA ACAGTTCTTC AGACTGATGG CCAACTAAAA 
ACTGGACGAG ATGTGGCCAT TGCAGCCCTT CTTGGCGCAG AAGAGTTTGG TTTCAGCACT 
GCACCCCTTA TAACACTTGG TTGCATCATG ATGCGAAAGT GCCATAAGAA CACCTGCCCC 
GTAGGCATTG CTACTCAAGA TCCAGTGCTG AGGGAAAAGT TTGCTGGAGA ACCTGAACAT 
GTTATCAACT TCTTTTTTAT GCTAGCAGAG GAGCTCAGGG AGATTATGGC TCAGCTTGGT 
TTCCGTACGA TGACTGAGAT GGTTGGTCGT TCAGACATGC TTGAAGTTGA TAAAGAAGTT 
GTTAAGAGCA ACGAGAAGCT GGAAAATATT GATCTCTCTT TGTTACTTAG ACCTGCTGCT 
GACATTCGAC CTGAAGCTGC TCAGTATTGT GTCCAAAAGC AGGATCATGG TTTGGACATG 
GCATTGGATA ATAAACTCAT TAAACTCTCT GAGGCTGCAT TGGAAAAAGG TCTTCCTGTG 
TACATTGAAA CACCTATCTG CAATGTGAAC CGTGCTGTTG GAACGATGCT TAGTCATGAA 
GTTACTAAGC GTTACCACTT GGCAGGGCTT CCCGCAGATA CTATTCACAT CAAACTCACA 
GGAAGTGCTG GGCAGAGTCT AGGAGCTTTC CTTTGCCCTG GCATTATGCT GGAACTGGAA 
GGGGATGGCA ATGACTACGT TGGTAAAGGA TTATCAGGTG GGAAGATTGT AGTTTATCCT 
CCAAAAGGAA GCCTGTTTGA TCCAAAAGAA AACATTGTGA TCGGTAATGT AGCTCTTTAT 
GGAGCCACAT GTGGTGAAGC ATATTTCAAT GGGATGGCAG CAGAAAGATT TTGTGTCCGT 
AATTCTGGGG CAAGGGCTGT TGTAGAAGGT GTTGGTGATC ATGGCTGTGA GTACATGACA 
GGTGGAACTG TTGTTGTGCT TGGGAAAACT GGAAGGAATT TTGCTGCTGG TATGAGTGGT 
GGTGTTGCTT ATGTTCTTGA TTTGGATGGG AAATTCAGAT CTCGTTGCAA TCCTGAACTC 
GTAGATCTTG ACAAAGTCGA GGAAGAGGAG GACATTACAA CTCTAAAAAT GATGATACAA 
CAACACCAGC GTCACACAAA TAGCCTGCTA GCTAGAGAAG TACTTGCTGA TTTTGATAAT 
CTTCTACCTA AATTTATTAA GGTCTTCCCG AGGGATTACA AACGTGTTCT TGCCAACATG 
AAAGAGGAAT CTGCCACAAA AGAGGCTGCT GACCTAGCTG CTAAAGAAGT TGAGGAAGCT 
GAGGAGCAAG ATGAAGCAGA GTTGAAGGAG AAAGATGCTT TTGAGGAGCT AAAGAAGCTG 
GCAGCTGCAT CTTTGAATGG GAATTCCATT CAGGTAGAAG ATGGACCTCT GAAAAGGCCT 
ACCCGTGTTA ATGATGCTGT TAAACATCGA GGGTTTATTG CTTATGAGCG AGAAGGTGTT 
CAGTACAGAG ATCCAAATAT TCGAATGAAT GACTGGAAGG AAGTTACTGA GGAGTCAAAA 
CCTGGCCCTC TTTTAAAGAC CCAGTCAGCC CGTTGCATGG ACTGTGGCAC TCCTTTCTGT 
CACCAGGAGA ACTCAGGATG CCCTCTTGGA AACAAAATCC CTGAATTTAA TGAGCTAGTG 
CATCAAAATC GGTGGCGTGA AGCATTAGAT AGGTTACTTG AGACAAATAA CTTCCCAGAG 
TTCACTGGCA GAGTGTGCCC TGCACCCTGT GAAGGTTCCT GTGTTCTTGG GATAATTGAT 
GATCCAGTAT CTATAAAAAA CATTGAATGT TCAATTATTG ACAAGGCCTT TGAGGAGGGT 
TGGATGGTTC CGCGGCCTCC CCTCAAGAGA ACTGGGAGAC GAGTGGCTAT TGTTGGAAGC 
GGGCCTTCTG GTTTGGCTGC TGCTGATCAA CTAAACAAAA TGGGTCATTT GGTGACTGTG 
TATGAGCGTG CTGATCGAAT TGGAGGGCTA ATGATGTATG GAGTTCCCAA CATGAAAACT 
GACAAAGTGG ATATAGTTCA ACGGCGTGTT AACCTTATGT CTGAGGAAGG CATCAATTTT 
GTGGTAAATG CTAATGTTGG AATTGATCCT TTGTATTCTC TTGATCGGCT TCGAGATGAG 
AACAATGCGA TTGTTTTGGC AGTGGGAGCC ACAAAACCAA GGGACCTTCC GGTACCTGGT 
CGGGAGTTGT CAGGCGTCCA TTTTGCTATG CAGTTTCTTC ATGCAAATAC CAAAAGTTTG 
CTTGACAGCA ATTTGCAGGA TGGTAACTAC ATATCCGCAA ATGGCAAGAA AGTTGTTGTC 
ATTGGTGGAG GTGACACTGG AACAGATTGC ATTGGGACAT CTATTCGCCA TGGGTGTAGT 
AGCATTGTAA ATCTAGAGCT TCTACCTGAG CCACCACGAA CTAGAGGTCC GGGCAACCCT 
TGGCCACAGT GGCCTAGAGT ATTCCGGGTA GACTATGGGC ACCAGGAAGC TGCCGCCAAG 
TTCGGGAAAG ACCCGAGGTC TTATGAGGTG TTGACTAAGC GTTTCATTGG AGATGAGAAT 
GGGAATGTGA AAGGACTTGA GCTGGTACGT GTCCATTGGG AGAAGGATGC CACAGGGAAA 
TTTCAATTTA AGGAGGTTGA GGGCTCCGAG GAAGTTATTG AGGCTGACCT AGTCCTACTA 
GCAATGGGAT TCCTTGGTCC TGAGCTGAAT GTGGCAGAGA AGTTGGGCTT GGAGCAAGAT 
AATAGATCGA ATTTCAAGGC AGAATATGGC CGCTTCTCAA CCAATGTGGA AGGGATCTTT 
GCAGCTGGGG ATTGCAGGCG AGGCCAGTCC CTAGTTGTAT GGGCAATATC AGAGGGACGA 
CAAGCTGCTT CCCAGGTGGA CAAATATCTC ATGAAAGAAG AGGATGCTAC CATTAACACC 
GATAATACTC AGGATGACCT TGTCAAGAGG CACCAGGACC TCACCAAGAG ACATCAAGAC 
AGCAGCAAGC ACACTGTCAT GACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC7453835 ( XM_002321983.3 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_002322019.3 Populus trichocarpa glutamate synthase [NADH], amyloplastic (LOC7453835), transcript variant X1, mRNA.

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CloneID	OPa444171
Clone ID Related Accession (Same CDS sequence)	XM_002321983.3
Accession Version	XM_002321983.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523116800000
Organism	Populus trichocarpa(black cottonwood)
Product	glutamate synthase [NADH], amyloplastic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037299.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 9, 2018 this sequence version replaced XM_002321983.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCTG CAGCGAGCTC TGGAGCTCTC CTTCAACCAA AATCGGTGAA AGCTCCGTTT 
TCTTCGCTTA GTAAATCGTC TTCTCTTTCT CCTTCGCTCA ATGTTGCCAC AGCAGCATCT 
GTTTCTCGGC GGAGCGCGAG GGCAAACCGA TGTGCTTCGA CGAGGAAATC GGTGGTTGTT 
GAGAGAAAAT CGTTTTTGGG AAGTAAAGTG CGGGGGTCTG CTGGATCGGA GAGATTGCAT 
TTTTGGCAGT CGGATGGACC GGGTAGAGAG CCGAAACTTC GAGTTGTTGT CCGGTCTGCA 
TTGTCCGGTG TGCCGGAGAA GCCGCTTGGG TTGTATGACC CGTCGTTTGA TAAGGATTCG 
TGCGGGGTTG GTTTTGTGGC TGAATTATCT GGGGATAGCA GTCGTAAAAC GGTAAATGAT 
GCTTTGGAGA TGTTGGTGAG GATGACACAT AGAGGGGCTT GTGGATGTGA GACGAATACT 
GGTGATGGGG CTGGTATTCT TGTGGCTCTT CCTCATGATT TCTACAAGGA GGTTGCTAAG 
GATATAGGGT TCGAACTGCC ACCACCAGGG GAATATGCAG TTGGCATGTT CTTTTTGCCC 
ACATCTGATA ATCGTAAGGA AGAAAGCAAG AATGTATTCA CTAAGGTTGC GGAGTCTCTT 
GGGCATACTG TACTTGGGTG GCGACCAGTA CCAACAGATA ACTCTGGACT GGGAAATTCT 
GCTTTGCAGA CCGAGCCTGT CATTGAGCAA GTGTTCCTTA CAGCCACTCC TAGGTCGAAG 
GCTGATTTTG AGCAGCAGAT GTACATATTA AGGAGGGTGT CAATGGTAGC TATTAGAGCT 
GCACTAAACC TTCAATATGG TGGTGTTAGG GACTTTTATA TCTGTTCCCT TTCCTCAAGG 
ACGGTTGTTT ATAAAGGTCA GTTGAAGCCT GAACAGTTGA AGGGTTACTA TTATGCAGAT 
CTTGGCAATG AAAGGTTTAC GAGCTACATG GCCCTGGTAC ACTCACGATT TTCAACAAAT 
ACATTTCCTA GCTGGGACCG TGCACAACCC ATGCGTGTCT TGGGCCATAA TGGGGAAATC 
AACACGCTAA GAGGCAATGT AAACTGGATG AAGGCTCGCG AGGGCCTAAT AAAGTGCAAG 
GAGCTCGGTC TCTCGAAGAA TGAGATGAAG AAGCTTCTTC CAATTGTAGA TGCTAGTTCT 
TCCGACTCAG GGGCTTTTGA TGGTGTGCTG GAGCTTTTAA TTCGAGCTGG GAGAAGTCTC 
CCTGAAGCTG TCATGATGAT GATTCCTGAA GCCTGGCAAA ATGACAAGAA TATGGATCCT 
CAAAGGAGGG CACTGTACGA ATACTTCTCA GCTCTTATGG AACCGTGGGA TGGGCCTGCA 
CTTATCTCAT TTACTGATGG CCACTACTTG GGAGCTACTC TGGATCGCAA TGGGTTACGT 
CCAGGAAGAT TTTATGTTAC ACGAAGTGGA CGAGTTATCA TGGCTAGTGA AGTTGGTGTT 
GTGGATATTC CTCCTGAAGA TGTCCTTAGG AAAGGAAGAC TTAACCCTGG AATGATGCTT 
CTGGTGGATT TTGAGAAGCA TATTATTGTA GATGATGAAG CCTTGAAGCA ACAATATTCC 
CTAGCAAGGC CCTATGGAGA GTGGCTGAAG AGGCAAAAGA TTGAACTCAG TGACATAGTT 
GACTCGGTTC AAGAATCTGA GAGAGTTGCT CCAGCCATTT CTGGGGTTGT TCCTGCATCT 
GATGATGACA CCAGCATGCA GAATATGGGC ACTCATGGTT TGCTGGCACC ATTAAAAGCT 
TTTGGCTATA CTGTTGAAGC CTTGGAGATG CTGATGCTTC CCATGGCAAA GGACGCTACA 
GAGGCTCTTG GTTCGATGGG AAATGATGCT CCCTTGGCTG TCATGTCTAA CAGGGAAAAA 
CTCACTTTCG AGTACTTCAA GCAAATGTTT GCTCAAGTGA CAAACCCTCC AATTGATCCC 
ATCAGAGAGA AGATTGTCAC TTCCATGGAA TGCATGATTG GGCCAGAAGG TGATTTGACA 
GAAACAACTG AAGAACAATG CCACCGTCTC TCTCTAAAAG GCCCTCTTTT ATCAATTGAG 
CAAATGGAAG CAATGAAAAA AATGAACTTT AGCGGTTGGC GAAGCAAAGT TCTCGACATA 
ACTTATTCAA AGGAACGGGG TAGGAAGGGA TTGGAGGAGA CTTTAGATAG GATTTGTGCT 
GAAGCTCACG AGGCAATCAA GGAAGGTTAT ACTGTGTTGG TTCTTTCTGA CAGAGCCTTC 
TCATCAAAGC GTGTTGCTGC AAGCTCCCTC TTGGCTGTTG GCGCTGTCCA CCAATATCTA 
GTAAAAAAGC TTGAGCGAAC TCAAGTCGGG TTGATAGTTG AATCTGCTGA ACCACGTGAA 
GTGCACCATT TCTGTACGTT GGTTGGTTTT GGCGCAGATG CTATCTGCCC ATACTTAGCT 
GTAGAGGCCA TTTGGAGATT ACAGGTTGAT GGCAAGATCC CACCAAAATC CACTGGCGAG 
TTCCACACAA AGGATGAGCT AGTCAAGAAG TATTTCAAGG CAAGCAACTA TGGCATGATG 
AAAGTGCTTG CCAAAATGGG AATTTCAACT TTGGCCTCCT ACAAGGGTGC TCAGATTTTT 
GAAGGCCTGG GCCTTTCATC AGAAGTGATT GACAAGTGCT TTGCAGGAAC TCCAAGCAGG 
GTAGAAGGAG CAACATTTGA GATGCTTGCC CGTGATTCAC TTCATTTGCA TGAGTTGGCA 
TTTCCTTCCC GTGTTCTCCC TCCTGGAAGT GCAGAAGCTG TAGCACTGCC CAATCCGGGG 
GATTATCATT GGAGAAAAGG TGGTGAGATT CACCTTAATG ATCCTCTTGC TATTGCAAAG 
CTTCAAGAAG CTGCCAGAGG TAATAGTGTG GCTGCCTATA AGGAATATTC TAAACGTGTT 
CAGGAGTTAA ACAAAGCTTG TAATTTGCGT GGGCTTTTGA AGTTCAAAGA GGCAGATGTG 
AAAGTTTCTT TGGATGAAGT AGAACCAGCC AGTGAGATTG TTAAAAGGTT CTGTACTGGT 
GCAATGAGTT ATGGATCAAT ATCATTGGAG GCACACACCA CACTGGCTCA GGCTATGAAC 
AAAATTGGAG GAAAATCAAA CACAGGTGAG GGAGGTGAAC AACCATCTCG GATGGAGACT 
CTTCCTGATG GTTCAATGAA CCCAAAGAGG AGTGCAATTA AGCAGGTTGC AAGTGGGAGA 
TTTGGAGTGT CAAGTTACTA TCTTACAAAT GCTGATGAGC TGCAGATAAA GATGGCTCAG 
GGTGCCAAGC CTGGTGAAGG AGGTGAGCTT CCTGGCCACA AGGTCATAGG AGACATTGCA 
ATTACCAGAA ATTCTACTGC AGGGGTGGGC CTCATTAGTC CTCCTCCCCA TCATGATATC 
TATTCAATTG AAGACCTTGC TCAATTGATT CACGATCTCA AGAATGCCAA TCCAGCAGCC 
AGAATAAGTG TTAAGTTGGT ATCTGAAGCT GGTGTCGGAG TCATTGCCAG TGGGGTGGTA 
AAGGGACATG CTGACCATGT TTTGATCTCA GGTCATGATG GAGGTACAGG TGCTTCTCGA 
TGGACTGGCA TCAAGAACGC TGGGCTCCCA TGGGAACTTG GTTTGGCTGA GACCCATCAA 
ACACTTGTTG CCAATGACCT TCGTGGCCGA ACAGTTCTTC AGACTGATGG CCAACTAAAA 
ACTGGACGAG ATGTGGCCAT TGCAGCCCTT CTTGGCGCAG AAGAGTTTGG TTTCAGCACT 
GCACCCCTTA TAACACTTGG TTGCATCATG ATGCGAAAGT GCCATAAGAA CACCTGCCCC 
GTAGGCATTG CTACTCAAGA TCCAGTGCTG AGGGAAAAGT TTGCTGGAGA ACCTGAACAT 
GTTATCAACT TCTTTTTTAT GCTAGCAGAG GAGCTCAGGG AGATTATGGC ACAGCTTGGT 
TTCCGTACGA TGACTGAGAT GGTTGGTCGT TCAGACATGC TTGAAGTTGA TAAAGAAGTT 
GTTAAGAGCA ACGAGAAGCT GGAAAATATT GATCTCTCTT TGTTACTTAG ACCTGCTGCT 
GACATTCGAC CTGAAGCTGC TCAGTATTGT GTCCAAAAGC AGGATCATGG TTTGGACATG 
GCATTGGATA ATAAACTCAT TAAACTCTCT GAGGCTGCAT TGGAAAAAGG TCTTCCTGTA 
TACATTGAAA CACCTATCTG CAATGTGAAC CGTGCTGTTG GAACGATGCT TAGTCATGAA 
GTTACTAAGC GTTACCACTT GGCAGGGCTT CCCGCAGATA CTATTCACAT CAAACTCACA 
GGAAGTGCTG GGCAGAGTCT AGGAGCTTTC CTTTGCCCTG GCATTATGCT GGAACTGGAA 
GGGGATGGCA ATGACTACGT TGGTAAAGGA TTATCAGGTG GGAAGATTGT AGTTTATCCT 
CCAAAAGGAA GCCTGTTTGA TCCAAAAGAA AACATTGTGA TCGGTAATGT AGCTCTTTAT 
GGAGCCACAT GTGGTGAAGC ATATTTCAAT GGGATGGCAG CAGAAAGATT TTGTGTCCGT 
AATTCTGGGG CAAGGGCTGT TGTAGAAGGT GTTGGTGATC ATGGCTGTGA GTACATGACA 
GGTGGAACTG TTGTTGTGCT TGGGAAAACT GGAAGGAATT TTGCTGCTGG TATGAGTGGT 
GGTGTTGCTT ATGTTCTTGA TTTGGATGGG AAATTCAGAT CTCGTTGCAA TCCTGAACTC 
GTAGATCTTG ACAAAGTCGA GGAAGAGGAG GACATTACAA CTCTAAAAAT GATGATACAA 
CAACACCAGC GTCACACAAA TAGCCTGCTA GCTAGAGAAG TACTTGCTGA TTTTGATAAT 
CTTCTACCTA AATTTATTAA GGTCTTCCCG AGGGATTACA AACGTGTTCT TGCCAACATG 
AAAGAGGAAT CTGCCACAAA AGAGGCTGCT GACCTAGCTG CTAAAGAAGT TGAGGAAGCT 
GAGGAGCAAG ATGAAGCAGA GTTGAAGGAG AAAGATGCTT TTGAGGAGCT AAAGAAGCTG 
GCAGCTGCAT CTTTGAATGG GAATTCCATT CAGGTAGAAG ATGGACCTCT GAAAAGGCCT 
ACCCGTGTTA ATGATGCTGT TAAACATCGA GGGTTTATTG CTTATGAGCG AGAAGGTGTT 
CAGTACAGAG ATCCAAATAT TCGAATGAAT GACTGGAAGG AAGTTACTGA GGAGTCAAAA 
CCTGGCCCTC TTTTAAAGAC CCAGTCAGCC CGTTGCATGG ACTGTGGCAC TCCTTTCTGT 
CACCAGGAGA ACTCAGGATG CCCTCTTGGA AACAAAATCC CTGAATTTAA TGAGCTAGTG 
CATCAAAATC GGTGGCGTGA AGCATTAGAT AGGTTACTTG AGACAAATAA CTTCCCAGAG 
TTCACTGGCA GAGTGTGCCC TGCACCCTGT GAAGGTTCCT GTGTTCTTGG GATAATTGAT 
GATCCAGTAT CTATAAAAAA CATTGAATGT TCAATTATTG ACAAGGCCTT TGAGGAGGGT 
TGGATGGTTC CGCGGCCTCC CCTCAAGAGA ACTGGGAGAC GAGTGGCTAT TGTTGGAAGC 
GGGCCTTCTG GTTTGGCTGC TGCTGATCAA CTAAACAAAA TGGGTCATTT GGTGACTGTG 
TATGAGCGTG CTGATCGAAT TGGAGGGCTA ATGATGTATG GAGTTCCCAA CATGAAAACT 
GACAAAGTGG ATATAGTTCA ACGGCGTGTT AACCTTATGT CTGAGGAAGG CATCAATTTT 
GTGGTAAATG CTAATGTTGG AATTGATCCT TTGTATTCTC TTGATCGGCT TCGAGATGAG 
AACAATGCGA TTGTTTTGGC AGTGGGAGCC ACAAAACCAA GGGACCTTCC GGTACCTGGT 
CGGGAGTTGT CAGGCGTCCA TTTTGCTATG CAGTTTCTTC ATGCAAATAC CAAAAGTTTG 
CTTGACAGCA ATTTGCAGGA TGGTAACTAC ATATCCGCAA AGGGCAAGAA AGTTGTTGTC 
ATTGGTGGAG GTGACACTGG AACAGATTGC ATTGGGACAT CTATTCGCCA TGGGTGTAGT 
AGCATTGTAA ATCTAGAGCT TCTACCTGAG CCACCACGAA CTAGAGGTCC GGGCAACCCT 
TGGCCACAGT GGCCTAGAGT ATTCCGGGTA GACTATGGGC ACCAGGAAGC TGCCGCCAAG 
TTCGGGAAAG ACCCGAGGTC TTATGAGGTG TTGACTAAGC GTTTCATTGG AGATGAGAAT 
GGGAATGTGA AAGGACTTGA GCTGGTACGT GTCCATTGGG AGAAGGATGC CACAGGGAAA 
TTTCAATTTA AGGAGGTTGA GGGCTCCGAG GAAGTTATTG AGGCTGACCT AGTCCTACTA 
GCAATGGGAT TCCTTGGTCC TGAGCTGAAT GTGGCAGAGA AGTTGGGCTT GGAGCAAGAT 
AATAGATCGA ATTTCAAGGC AGAATATGGC CGCTTCTCAA CCAATGTGGA AGGGATCTTT 
GCAGCTGGGG ATTGCAGGCG AGGCCAGTCC CTAGTTGTAT GGGCAATATC AGAGGGACGA 
CAAGCTGCTT CCCAGGTGGA CAAATATCTC ATGAAAGAAG AGGATGCTAC CATTAACACC 
GATAATACTC AGGATGACCT TGTCAAGAGG CACCAGGACC TCACCAAGAG ACATCAAGAC 
AGCAGCAAGC ACACTGTCAT GACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002322019.3
CDS	359..7045
Translation	MSAAASSGALLQPKSVKAPFSSLSKSSSLSPSLNVATAASVSRRSARANRCASTRKSVVVERKSFLGSKVRGSAGSERLHFWQSDGPGREPKLRVVVRSALSGVPEKPLGLYDPSFDKDSCGVGFVAELSGDSSRKTVNDALEMLVRMTHRGACGCETNTGDGAGILVALPHDFYKEVAKDIGFELPPPGEYAVGMFFLPTSDNRKEESKNVFTKVAESLGHTVLGWRPVPTDNSGLGNSALQTEPVIEQVFLTATPRSKADFEQQMYILRRVSMVAIRAALNLQYGGVRDFYICSLSSRTVVYKGQLKPEQLKGYYYADLGNERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLIKCKELGLSKNEMKKLLPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNMDPQRRALYEYFSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTRSGRVIMASEVGVVDIPPEDVLRKGRLNPGMMLLVDFEKHIIVDDEALKQQYSLARPYGEWLKRQKIELSDIVDSVQESERVAPAISGVVPASDDDTSMQNMGTHGLLAPLKAFGYTVEALEMLMLPMAKDATEALGSMGNDAPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAMKKMNFSGWRSKVLDITYSKERGRKGLEETLDRICAEAHEAIKEGYTVLVLSDRAFSSKRVAASSLLAVGAVHQYLVKKLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPPKSTGEFHTKDELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEGLGLSSEVIDKCFAGTPSRVEGATFEMLARDSLHLHELAFPSRVLPPGSAEAVALPNPGDYHWRKGGEIHLNDPLAIAKLQEAARGNSVAAYKEYSKRVQELNKACNLRGLLKFKEADVKVSLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAQAMNKIGGKSNTGEGGEQPSRMETLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITLGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMLAEELREIMAQLGFRTMTEMVGRSDMLEVDKEVVKSNEKLENIDLSLLLRPAADIRPEAAQYCVQKQDHGLDMALDNKLIKLSEAALEKGLPVYIETPICNVNRAVGTMLSHEVTKRYHLAGLPADTIHIKLTGSAGQSLGAFLCPGIMLELEGDGNDYVGKGLSGGKIVVYPPKGSLFDPKENIVIGNVALYGATCGEAYFNGMAAERFCVRNSGARAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGVAYVLDLDGKFRSRCNPELVDLDKVEEEEDITTLKMMIQQHQRHTNSLLAREVLADFDNLLPKFIKVFPRDYKRVLANMKEESATKEAADLAAKEVEEAEEQDEAELKEKDAFEELKKLAAASLNGNSIQVEDGPLKRPTRVNDAVKHRGFIAYEREGVQYRDPNIRMNDWKEVTEESKPGPLLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVHQNRWREALDRLLETNNFPEFTGRVCPAPCEGSCVLGIIDDPVSIKNIECSIIDKAFEEGWMVPRPPLKRTGRRVAIVGSGPSGLAAADQLNKMGHLVTVYERADRIGGLMMYGVPNMKTDKVDIVQRRVNLMSEEGINFVVNANVGIDPLYSLDRLRDENNAIVLAVGATKPRDLPVPGRELSGVHFAMQFLHANTKSLLDSNLQDGNYISAKGKKVVVIGGGDTGTDCIGTSIRHGCSSIVNLELLPEPPRTRGPGNPWPQWPRVFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGNVKGLELVRVHWEKDATGKFQFKEVEGSEEVIEADLVLLAMGFLGPELNVAEKLGLEQDNRSNFKAEYGRFSTNVEGIFAAGDCRRGQSLVVWAISEGRQAASQVDKYLMKEEDATINTDNTQDDLVKRHQDLTKRHQDSSKHTVMT

Target ORF information:

RefSeq Version	XM_002321983.3
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa glutamate synthase [NADH], amyloplastic (LOC7453835), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002321983.3

ORF Insert Sequence:

ATGTCGGCTG CAGCGAGCTC TGGAGCTCTC CTTCAACCAA AATCGGTGAA AGCTCCGTTT 
TCTTCGCTTA GTAAATCGTC TTCTCTTTCT CCTTCGCTCA ATGTTGCCAC AGCAGCATCT 
GTTTCTCGGC GGAGCGCGAG GGCAAACCGA TGTGCTTCGA CGAGGAAATC GGTGGTTGTT 
GAGAGAAAAT CGTTTTTGGG AAGTAAAGTG CGGGGGTCTG CTGGATCGGA GAGATTGCAT 
TTTTGGCAGT CGGATGGACC GGGTAGAGAG CCGAAACTTC GAGTTGTTGT CCGGTCTGCA 
TTGTCCGGTG TGCCGGAGAA GCCGCTTGGG TTGTATGACC CGTCGTTTGA TAAGGATTCG 
TGCGGGGTTG GTTTTGTGGC TGAATTATCT GGGGATAGCA GTCGTAAAAC GGTAAATGAT 
GCTTTGGAGA TGTTGGTGAG GATGACACAT AGAGGGGCTT GTGGATGTGA GACGAATACT 
GGTGATGGGG CTGGTATTCT TGTGGCTCTT CCTCATGATT TCTACAAGGA GGTTGCTAAG 
GATATAGGGT TCGAACTGCC ACCACCAGGG GAATATGCAG TTGGCATGTT CTTTTTGCCC 
ACATCTGATA ATCGTAAGGA AGAAAGCAAG AATGTATTCA CTAAGGTTGC GGAGTCTCTT 
GGGCATACTG TACTTGGGTG GCGACCAGTA CCAACAGATA ACTCTGGACT GGGAAATTCT 
GCTTTGCAGA CCGAGCCTGT CATTGAGCAA GTGTTCCTTA CAGCCACTCC TAGGTCGAAG 
GCTGATTTTG AGCAGCAGAT GTACATATTA AGGAGGGTGT CAATGGTAGC TATTAGAGCT 
GCACTAAACC TTCAATATGG TGGTGTTAGG GACTTTTATA TCTGTTCCCT TTCCTCAAGG 
ACGGTTGTTT ATAAAGGTCA GTTGAAGCCT GAACAGTTGA AGGGTTACTA TTATGCAGAT 
CTTGGCAATG AAAGGTTTAC GAGCTACATG GCCCTGGTAC ACTCACGATT TTCAACAAAT 
ACATTTCCTA GCTGGGACCG TGCACAACCC ATGCGTGTCT TGGGCCATAA TGGGGAAATC 
AACACGCTAA GAGGCAATGT AAACTGGATG AAGGCTCGCG AGGGCCTAAT AAAGTGCAAG 
GAGCTCGGTC TCTCGAAGAA TGAGATGAAG AAGCTTCTTC CAATTGTAGA TGCTAGTTCT 
TCCGACTCAG GGGCTTTTGA TGGTGTGCTG GAGCTTTTAA TTCGAGCTGG GAGAAGTCTC 
CCTGAAGCTG TCATGATGAT GATTCCTGAA GCCTGGCAAA ATGACAAGAA TATGGATCCT 
CAAAGGAGGG CACTGTACGA ATACTTCTCA GCTCTTATGG AACCGTGGGA TGGGCCTGCA 
CTTATCTCAT TTACTGATGG CCACTACTTG GGAGCTACTC TGGATCGCAA TGGGTTACGT 
CCAGGAAGAT TTTATGTTAC ACGAAGTGGA CGAGTTATCA TGGCTAGTGA AGTTGGTGTT 
GTGGATATTC CTCCTGAAGA TGTCCTTAGG AAAGGAAGAC TTAACCCTGG AATGATGCTT 
CTGGTGGATT TTGAGAAGCA TATTATTGTA GATGATGAAG CCTTGAAGCA ACAATATTCC 
CTAGCAAGGC CCTATGGAGA GTGGCTGAAG AGGCAAAAGA TTGAACTCAG TGACATAGTT 
GACTCGGTTC AAGAATCTGA GAGAGTTGCT CCAGCCATTT CTGGGGTTGT TCCTGCATCT 
GATGATGACA CCAGCATGCA GAATATGGGC ACTCATGGTT TGCTGGCACC ATTAAAAGCT 
TTTGGCTATA CTGTTGAAGC CTTGGAGATG CTGATGCTTC CCATGGCAAA GGACGCTACA 
GAGGCTCTTG GTTCGATGGG AAATGATGCT CCCTTGGCTG TCATGTCTAA CAGGGAAAAA 
CTCACTTTCG AGTACTTCAA GCAAATGTTT GCTCAAGTGA CAAACCCTCC AATTGATCCC 
ATCAGAGAGA AGATTGTCAC TTCCATGGAA TGCATGATTG GGCCAGAAGG TGATTTGACA 
GAAACAACTG AAGAACAATG CCACCGTCTC TCTCTAAAAG GCCCTCTTTT ATCAATTGAG 
CAAATGGAAG CAATGAAAAA AATGAACTTT AGCGGTTGGC GAAGCAAAGT TCTCGACATA 
ACTTATTCAA AGGAACGGGG TAGGAAGGGA TTGGAGGAGA CTTTAGATAG GATTTGTGCT 
GAAGCTCACG AGGCAATCAA GGAAGGTTAT ACTGTGTTGG TTCTTTCTGA CAGAGCCTTC 
TCATCAAAGC GTGTTGCTGC AAGCTCCCTC TTGGCTGTTG GCGCTGTCCA CCAATATCTA 
GTAAAAAAGC TTGAGCGAAC TCAAGTCGGG TTGATAGTTG AATCTGCTGA ACCACGTGAA 
GTGCACCATT TCTGTACGTT GGTTGGTTTT GGCGCAGATG CTATCTGCCC ATACTTAGCT 
GTAGAGGCCA TTTGGAGATT ACAGGTTGAT GGCAAGATCC CACCAAAATC CACTGGCGAG 
TTCCACACAA AGGATGAGCT AGTCAAGAAG TATTTCAAGG CAAGCAACTA TGGCATGATG 
AAAGTGCTTG CCAAAATGGG AATTTCAACT TTGGCCTCCT ACAAGGGTGC TCAGATTTTT 
GAAGGCCTGG GCCTTTCATC AGAAGTGATT GACAAGTGCT TTGCAGGAAC TCCAAGCAGG 
GTAGAAGGAG CAACATTTGA GATGCTTGCC CGTGATTCAC TTCATTTGCA TGAGTTGGCA 
TTTCCTTCCC GTGTTCTCCC TCCTGGAAGT GCAGAAGCTG TAGCACTGCC CAATCCGGGG 
GATTATCATT GGAGAAAAGG TGGTGAGATT CACCTTAATG ATCCTCTTGC TATTGCAAAG 
CTTCAAGAAG CTGCCAGAGG TAATAGTGTG GCTGCCTATA AGGAATATTC TAAACGTGTT 
CAGGAGTTAA ACAAAGCTTG TAATTTGCGT GGGCTTTTGA AGTTCAAAGA GGCAGATGTG 
AAAGTTTCTT TGGATGAAGT AGAACCAGCC AGTGAGATTG TTAAAAGGTT CTGTACTGGT 
GCAATGAGTT ATGGATCAAT ATCATTGGAG GCACACACCA CACTGGCTCA GGCTATGAAC 
AAAATTGGAG GAAAATCAAA CACAGGTGAG GGAGGTGAAC AACCATCTCG GATGGAGACT 
CTTCCTGATG GTTCAATGAA CCCAAAGAGG AGTGCAATTA AGCAGGTTGC AAGTGGGAGA 
TTTGGAGTGT CAAGTTACTA TCTTACAAAT GCTGATGAGC TGCAGATAAA GATGGCTCAG 
GGTGCCAAGC CTGGTGAAGG AGGTGAGCTT CCTGGCCACA AGGTCATAGG AGACATTGCA 
ATTACCAGAA ATTCTACTGC AGGGGTGGGC CTCATTAGTC CTCCTCCCCA TCATGATATC 
TATTCAATTG AAGACCTTGC TCAATTGATT CACGATCTCA AGAATGCCAA TCCAGCAGCC 
AGAATAAGTG TTAAGTTGGT ATCTGAAGCT GGTGTCGGAG TCATTGCCAG TGGGGTGGTA 
AAGGGACATG CTGACCATGT TTTGATCTCA GGTCATGATG GAGGTACAGG TGCTTCTCGA 
TGGACTGGCA TCAAGAACGC TGGGCTCCCA TGGGAACTTG GTTTGGCTGA GACCCATCAA 
ACACTTGTTG CCAATGACCT TCGTGGCCGA ACAGTTCTTC AGACTGATGG CCAACTAAAA 
ACTGGACGAG ATGTGGCCAT TGCAGCCCTT CTTGGCGCAG AAGAGTTTGG TTTCAGCACT 
GCACCCCTTA TAACACTTGG TTGCATCATG ATGCGAAAGT GCCATAAGAA CACCTGCCCC 
GTAGGCATTG CTACTCAAGA TCCAGTGCTG AGGGAAAAGT TTGCTGGAGA ACCTGAACAT 
GTTATCAACT TCTTTTTTAT GCTAGCAGAG GAGCTCAGGG AGATTATGGC ACAGCTTGGT 
TTCCGTACGA TGACTGAGAT GGTTGGTCGT TCAGACATGC TTGAAGTTGA TAAAGAAGTT 
GTTAAGAGCA ACGAGAAGCT GGAAAATATT GATCTCTCTT TGTTACTTAG ACCTGCTGCT 
GACATTCGAC CTGAAGCTGC TCAGTATTGT GTCCAAAAGC AGGATCATGG TTTGGACATG 
GCATTGGATA ATAAACTCAT TAAACTCTCT GAGGCTGCAT TGGAAAAAGG TCTTCCTGTA 
TACATTGAAA CACCTATCTG CAATGTGAAC CGTGCTGTTG GAACGATGCT TAGTCATGAA 
GTTACTAAGC GTTACCACTT GGCAGGGCTT CCCGCAGATA CTATTCACAT CAAACTCACA 
GGAAGTGCTG GGCAGAGTCT AGGAGCTTTC CTTTGCCCTG GCATTATGCT GGAACTGGAA 
GGGGATGGCA ATGACTACGT TGGTAAAGGA TTATCAGGTG GGAAGATTGT AGTTTATCCT 
CCAAAAGGAA GCCTGTTTGA TCCAAAAGAA AACATTGTGA TCGGTAATGT AGCTCTTTAT 
GGAGCCACAT GTGGTGAAGC ATATTTCAAT GGGATGGCAG CAGAAAGATT TTGTGTCCGT 
AATTCTGGGG CAAGGGCTGT TGTAGAAGGT GTTGGTGATC ATGGCTGTGA GTACATGACA 
GGTGGAACTG TTGTTGTGCT TGGGAAAACT GGAAGGAATT TTGCTGCTGG TATGAGTGGT 
GGTGTTGCTT ATGTTCTTGA TTTGGATGGG AAATTCAGAT CTCGTTGCAA TCCTGAACTC 
GTAGATCTTG ACAAAGTCGA GGAAGAGGAG GACATTACAA CTCTAAAAAT GATGATACAA 
CAACACCAGC GTCACACAAA TAGCCTGCTA GCTAGAGAAG TACTTGCTGA TTTTGATAAT 
CTTCTACCTA AATTTATTAA GGTCTTCCCG AGGGATTACA AACGTGTTCT TGCCAACATG 
AAAGAGGAAT CTGCCACAAA AGAGGCTGCT GACCTAGCTG CTAAAGAAGT TGAGGAAGCT 
GAGGAGCAAG ATGAAGCAGA GTTGAAGGAG AAAGATGCTT TTGAGGAGCT AAAGAAGCTG 
GCAGCTGCAT CTTTGAATGG GAATTCCATT CAGGTAGAAG ATGGACCTCT GAAAAGGCCT 
ACCCGTGTTA ATGATGCTGT TAAACATCGA GGGTTTATTG CTTATGAGCG AGAAGGTGTT 
CAGTACAGAG ATCCAAATAT TCGAATGAAT GACTGGAAGG AAGTTACTGA GGAGTCAAAA 
CCTGGCCCTC TTTTAAAGAC CCAGTCAGCC CGTTGCATGG ACTGTGGCAC TCCTTTCTGT 
CACCAGGAGA ACTCAGGATG CCCTCTTGGA AACAAAATCC CTGAATTTAA TGAGCTAGTG 
CATCAAAATC GGTGGCGTGA AGCATTAGAT AGGTTACTTG AGACAAATAA CTTCCCAGAG 
TTCACTGGCA GAGTGTGCCC TGCACCCTGT GAAGGTTCCT GTGTTCTTGG GATAATTGAT 
GATCCAGTAT CTATAAAAAA CATTGAATGT TCAATTATTG ACAAGGCCTT TGAGGAGGGT 
TGGATGGTTC CGCGGCCTCC CCTCAAGAGA ACTGGGAGAC GAGTGGCTAT TGTTGGAAGC 
GGGCCTTCTG GTTTGGCTGC TGCTGATCAA CTAAACAAAA TGGGTCATTT GGTGACTGTG 
TATGAGCGTG CTGATCGAAT TGGAGGGCTA ATGATGTATG GAGTTCCCAA CATGAAAACT 
GACAAAGTGG ATATAGTTCA ACGGCGTGTT AACCTTATGT CTGAGGAAGG CATCAATTTT 
GTGGTAAATG CTAATGTTGG AATTGATCCT TTGTATTCTC TTGATCGGCT TCGAGATGAG 
AACAATGCGA TTGTTTTGGC AGTGGGAGCC ACAAAACCAA GGGACCTTCC GGTACCTGGT 
CGGGAGTTGT CAGGCGTCCA TTTTGCTATG CAGTTTCTTC ATGCAAATAC CAAAAGTTTG 
CTTGACAGCA ATTTGCAGGA TGGTAACTAC ATATCCGCAA AGGGCAAGAA AGTTGTTGTC 
ATTGGTGGAG GTGACACTGG AACAGATTGC ATTGGGACAT CTATTCGCCA TGGGTGTAGT 
AGCATTGTAA ATCTAGAGCT TCTACCTGAG CCACCACGAA CTAGAGGTCC GGGCAACCCT 
TGGCCACAGT GGCCTAGAGT ATTCCGGGTA GACTATGGGC ACCAGGAAGC TGCCGCCAAG 
TTCGGGAAAG ACCCGAGGTC TTATGAGGTG TTGACTAAGC GTTTCATTGG AGATGAGAAT 
GGGAATGTGA AAGGACTTGA GCTGGTACGT GTCCATTGGG AGAAGGATGC CACAGGGAAA 
TTTCAATTTA AGGAGGTTGA GGGCTCCGAG GAAGTTATTG AGGCTGACCT AGTCCTACTA 
GCAATGGGAT TCCTTGGTCC TGAGCTGAAT GTGGCAGAGA AGTTGGGCTT GGAGCAAGAT 
AATAGATCGA ATTTCAAGGC AGAATATGGC CGCTTCTCAA CCAATGTGGA AGGGATCTTT 
GCAGCTGGGG ATTGCAGGCG AGGCCAGTCC CTAGTTGTAT GGGCAATATC AGAGGGACGA 
CAAGCTGCTT CCCAGGTGGA CAAATATCTC ATGAAAGAAG AGGATGCTAC CATTAACACC 
GATAATACTC AGGATGACCT TGTCAAGAGG CACCAGGACC TCACCAAGAG ACATCAAGAC 
AGCAGCAAGC ACACTGTCAT GACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,Déjardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasjärvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Leplé JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouzé P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC7453835 cDNA ORF clone, Populus trichocarpa(black cottonwood)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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