FREM1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM1
Entrez Gene ID	100020241
Full Name	FRAS1 related extracellular matrix 1
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007499582.1	XP_007499644.1	FRAS1-related extracellular matrix protein 1 isoform X3
XM_007499583.2	XP_007499645.1	FRAS1-related extracellular matrix protein 1 isoform X3
XM_007499584.2	XP_007499646.1	FRAS1-related extracellular matrix protein 1 isoform X3
XM_007499581.2	XP_007499643.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_016424261.1	XP_016279747.1	FRAS1-related extracellular matrix protein 1 isoform X2
XM_016424262.1	XP_016279748.1	FRAS1-related extracellular matrix protein 1 isoform X3
XM_001372782.3	XP_001372819.1	FRAS1-related extracellular matrix protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following FREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc28696	XM_007499582.1 Latest version!	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMc28696	XM_016424262.1 Latest version!	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMc28696	XM_007499583.2 Latest version!	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMc28696	XM_007499584.2 Latest version!	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMc28695	XM_007499581.2 Latest version!	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc28695	XM_001372782.3 Latest version!	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc44199	XM_016424261.1 Latest version!	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM1 ( XM_007499582.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007499644.1 Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMc28696
Clone ID Related Accession (Same CDS sequence)	XM_007499582.1 , XM_007499583.2 , XM_007499584.2 , XM_016424262.1
Accession Version	XM_007499582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAC AAGCATCCAT GCTGAAGGCT ACCTTGCCTC TCTTTGCCAG ATTCGTCTTG 
AGCAATGGAC TAAAGACAAA GCATGGAGTG TTTAAGATCA AACTGGAGTC TATTGACCAA 
GCCTTGCCTG TTGTGACCCA GAACAAGGGG TTAAGAATAA TGGAAAGGAC CATGGCGCTC 
CTCTCTCCAG ACATCCTTCA ACTTTCTGAC CCAGACACTC CAACAGAAAA CCTCACCTTC 
CTCTTGGCCC AGCTCCCCCA GTATGGACAG CTCTATCTTC GGGGGATGGT TCTCACACAA 
CACAACTTTA CACAACAAGA AGTGGACAGT AAGAATGTGG CCTACCAACA CCTAGGAGGA 
AACTCACAGG TTGACCAGTT TACCTTTGTA GCTACTGATG GGACAAATCA AGGCTTTGTT 
GTGGACGGAA GAGTGCAGAA TGAACCTGTG TCTTTCATTA TTCAGGTGTC CCAGTTGGAT 
AAAAAAGCAC CACGCATCAT CCAGCTTCAG TGCCCTTCCC AAGTGGAGCT TCTGAAAAAT 
GGTTGCTATG GGATTTACAT AACATCTCGA GTCCTGAAGG CATCTGACCC TGATACAGAG 
GACAACCAAA TCATCTTTAA GATTTTACGA GGCCCCCAGT ACGGACATCT GGAAAATACA 
ACAACAGGTG AATTTATTCA GGAGAGATTC AGTCAAAAGG ACCTAAACAG TAAAATCATT 
CTTTATGTCA TCAATCCATC TTCAGAAATG AATTCGGATA ATTTGGAGTT TCAAGTCACA 
GACCCCAGTG GAAACACCGC TGCCCCTCAA ATTTTGGAAC TAAAGTGGTC CCACATTCAT 
TTGCCCCAGA CTGAATATGA AGTTTGTGAA AACATGAACA CAGTACCACT GAAAATCACT 
AGGATGGGCC ATTTAATGGA ATCAGCCTTT GTTGGAGTAA AGGTTAATCA AGTATCTGCT 
ACAATTGGAA AAGATTTCAC CGTGAGTCCA TCAAAGCTGA TTCAGTTTGA TCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTCAACTC CCCTGTGAAT GCCGTTCTTG GCACGAAGAC AAAAGCCAGA 
GTGAAAATTT TGGACTCAAA AGGAGGACAG TGCCTCCATT CACATTCCTC TGGCCAAAAC 
CAGTACAACT CATGGGGGAA AGGCATATGG CTCCCACTTT CCCCAGGCTC ATCATCACCC 
TCCATTTCTG ATGGGATTCA TTTGGAAGGA AGACACCCTT CATCTTCCAA AGAGGGGATA 
ACACACAGCA GAGGGGACAA GCTTCGGGGC TTTGACTTAA CTGATATTCC CAGGAAAAGG 
CTTAGGACAA TGGGAAATGG CAAAACAGTT CACCCATCAT CCATTTATAG AAATGAAACA 
GATATAATTT TTAGGTATCA TGGTATGGTT GGATTAAGAG TAGAAGATGA CAAATCTGCC 
ATAAATAGAG AGAAGACCAG AGGGTCTATA ACTAATCAGA GGCTACAAAA GAAAAGCAGG 
AACACAGAAC CACTATGGGC AGATAAGGTG GACCAGATGC CCTATTTACA CTCCCCTATT 
CAGGACCAGT CACCATCCTT TCCAAAAAAT TGCACATTAG AATTAAAGGG ACTCTTTCAT 
TTTGAGGAAA GCTTCCAAAA ATTATATCAT TGCAATGGAA CTTCTTGGAA GCCTTGGAGT 
CCAAAAGACC AGGATGGGAA TGAAAGAACC TGTCCCATTG GATGGAGCCA TCAAAGCAGC 
CATTGCTACT CATTGATTAC AGAGCAAAAG GTCACCTGGA ATATGGCTGC TCGGGCTTGC 
AGGGAACAAC ATCAAGGCAC CCTAGCAAGT GTGTTTACGA AACAGCATAT GCAGTGGCTA 
TGGGACTTCA GTGGAAGAAA ATCCTTCTGG ATAGGTTTGA ATGACCAAGA GAATGCAGAT 
CGCTGGGAAT GGGATGGAGG TGAACCTGTA ACCTTCACAA ACTGGAAGAG AGAGGCCCCC 
CCACATTTAA AGAAAAGAAA GAATTGCGTT TTGGTAAAAA GAAGAGGCAA GTGGCAAGCT 
AAAGACTGCA GGAAGGATAA AGCTCACAAC TACATGTGTG CCAGGAAACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007499644.1
CDS	95..2248
Translation	MVKQASMLKATLPLFARFVLSNGLKTKHGVFKIKLESIDQALPVVTQNKGLRIMERTMALLSPDILQLSDPDTPTENLTFLLAQLPQYGQLYLRGMVLTQHNFTQQEVDSKNVAYQHLGGNSQVDQFTFVATDGTNQGFVVDGRVQNEPVSFIIQVSQLDKKAPRIIQLQCPSQVELLKNGCYGIYITSRVLKASDPDTEDNQIIFKILRGPQYGHLENTTTGEFIQERFSQKDLNSKIILYVINPSSEMNSDNLEFQVTDPSGNTAAPQILELKWSHIHLPQTEYEVCENMNTVPLKITRMGHLMESAFVGVKVNQVSATIGKDFTVSPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKARVKILDSKGGQCLHSHSSGQNQYNSWGKGIWLPLSPGSSSPSISDGIHLEGRHPSSSKEGITHSRGDKLRGFDLTDIPRKRLRTMGNGKTVHPSSIYRNETDIIFRYHGMVGLRVEDDKSAINREKTRGSITNQRLQKKSRNTEPLWADKVDQMPYLHSPIQDQSPSFPKNCTLELKGLFHFEESFQKLYHCNGTSWKPWSPKDQDGNERTCPIGWSHQSSHCYSLITEQKVTWNMAARACREQHQGTLASVFTKQHMQWLWDFSGRKSFWIGLNDQENADRWEWDGGEPVTFTNWKREAPPHLKKRKNCVLVKRRGKWQAKDCRKDKAHNYMCARKL

Target ORF information:

RefSeq Version	XM_007499582.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007499582.1

ORF Insert Sequence:

ATGGTGAAAC AAGCATCCAT GCTGAAGGCT ACCTTGCCTC TCTTTGCCAG ATTCGTCTTG 
AGCAATGGAC TAAAGACAAA GCATGGAGTG TTTAAGATCA AACTGGAGTC TATTGACCAA 
GCCTTGCCTG TTGTGACCCA GAACAAGGGG TTAAGAATAA TGGAAAGGAC CATGGCGCTC 
CTCTCTCCAG ACATCCTTCA ACTTTCTGAC CCAGACACTC CAACAGAAAA CCTCACCTTC 
CTCTTGGCCC AGCTCCCCCA GTATGGACAG CTCTATCTTC GGGGGATGGT TCTCACACAA 
CACAACTTTA CACAACAAGA AGTGGACAGT AAGAATGTGG CCTACCAACA CCTAGGAGGA 
AACTCACAGG TTGACCAGTT TACCTTTGTA GCTACTGATG GGACAAATCA AGGCTTTGTT 
GTGGACGGAA GAGTGCAGAA TGAACCTGTG TCTTTCATTA TTCAGGTGTC CCAGTTGGAT 
AAAAAAGCAC CACGCATCAT CCAGCTTCAG TGCCCTTCCC AAGTGGAGCT TCTGAAAAAT 
GGTTGCTATG GGATTTACAT AACATCTCGA GTCCTGAAGG CATCTGACCC TGATACAGAG 
GACAACCAAA TCATCTTTAA GATTTTACGA GGCCCCCAGT ACGGACATCT GGAAAATACA 
ACAACAGGTG AATTTATTCA GGAGAGATTC AGTCAAAAGG ACCTAAACAG TAAAATCATT 
CTTTATGTCA TCAATCCATC TTCAGAAATG AATTCGGATA ATTTGGAGTT TCAAGTCACA 
GACCCCAGTG GAAACACCGC TGCCCCTCAA ATTTTGGAAC TAAAGTGGTC CCACATTCAT 
TTGCCCCAGA CTGAATATGA AGTTTGTGAA AACATGAACA CAGTACCACT GAAAATCACT 
AGGATGGGCC ATTTAATGGA ATCAGCCTTT GTTGGAGTAA AGGTTAATCA AGTATCTGCT 
ACAATTGGAA AAGATTTCAC CGTGAGTCCA TCAAAGCTGA TTCAGTTTGA TCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTCAACTC CCCTGTGAAT GCCGTTCTTG GCACGAAGAC AAAAGCCAGA 
GTGAAAATTT TGGACTCAAA AGGAGGACAG TGCCTCCATT CACATTCCTC TGGCCAAAAC 
CAGTACAACT CATGGGGGAA AGGCATATGG CTCCCACTTT CCCCAGGCTC ATCATCACCC 
TCCATTTCTG ATGGGATTCA TTTGGAAGGA AGACACCCTT CATCTTCCAA AGAGGGGATA 
ACACACAGCA GAGGGGACAA GCTTCGGGGC TTTGACTTAA CTGATATTCC CAGGAAAAGG 
CTTAGGACAA TGGGAAATGG CAAAACAGTT CACCCATCAT CCATTTATAG AAATGAAACA 
GATATAATTT TTAGGTATCA TGGTATGGTT GGATTAAGAG TAGAAGATGA CAAATCTGCC 
ATAAATAGAG AGAAGACCAG AGGGTCTATA ACTAATCAGA GGCTACAAAA GAAAAGCAGG 
AACACAGAAC CACTATGGGC AGATAAGGTG GACCAGATGC CCTATTTACA CTCCCCTATT 
CAGGACCAGT CACCATCCTT TCCAAAAAAT TGCACATTAG AATTAAAGGG ACTCTTTCAT 
TTTGAGGAAA GCTTCCAAAA ATTATATCAT TGCAATGGAA CTTCTTGGAA GCCTTGGAGT 
CCAAAAGACC AGGATGGGAA TGAAAGAACC TGTCCCATTG GATGGAGCCA TCAAAGCAGC 
CATTGCTACT CATTGATTAC AGAGCAAAAG GTCACCTGGA ATATGGCTGC TCGGGCTTGC 
AGGGAACAAC ATCAAGGCAC CCTAGCAAGT GTGTTTACGA AACAGCATAT GCAGTGGCTA 
TGGGACTTCA GTGGAAGAAA ATCCTTCTGG ATAGGTTTGA ATGACCAAGA GAATGCAGAT 
CGCTGGGAAT GGGATGGAGG TGAACCTGTA ACCTTCACAA ACTGGAAGAG AGAGGCCCCC 
CCACATTTAA AGAAAAGAAA GAATTGCGTT TTGGTAAAAA GAAGAGGCAA GTGGCAAGCT 
AAAGACTGCA GGAAGGATAA AGCTCACAAC TACATGTGTG CCAGGAAACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_007499583.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007499645.1 Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMc28696
Clone ID Related Accession (Same CDS sequence)	XM_007499582.1 , XM_007499583.2 , XM_007499584.2 , XM_016424262.1
Accession Version	XM_007499583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007499583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAC AAGCATCCAT GCTGAAGGCT ACCTTGCCTC TCTTTGCCAG ATTCGTCTTG 
AGCAATGGAC TAAAGACAAA GCATGGAGTG TTTAAGATCA AACTGGAGTC TATTGACCAA 
GCCTTGCCTG TTGTGACCCA GAACAAGGGG TTAAGAATAA TGGAAAGGAC CATGGCGCTC 
CTCTCTCCAG ACATCCTTCA ACTTTCTGAC CCAGACACTC CAACAGAAAA CCTCACCTTC 
CTCTTGGCCC AGCTCCCCCA GTATGGACAG CTCTATCTTC GGGGGATGGT TCTCACACAA 
CACAACTTTA CACAACAAGA AGTGGACAGT AAGAATGTGG CCTACCAACA CCTAGGAGGA 
AACTCACAGG TTGACCAGTT TACCTTTGTA GCTACTGATG GGACAAATCA AGGCTTTGTT 
GTGGACGGAA GAGTGCAGAA TGAACCTGTG TCTTTCATTA TTCAGGTGTC CCAGTTGGAT 
AAAAAAGCAC CACGCATCAT CCAGCTTCAG TGCCCTTCCC AAGTGGAGCT TCTGAAAAAT 
GGTTGCTATG GGATTTACAT AACATCTCGA GTCCTGAAGG CATCTGACCC TGATACAGAG 
GACAACCAAA TCATCTTTAA GATTTTACGA GGCCCCCAGT ACGGACATCT GGAAAATACA 
ACAACAGGTG AATTTATTCA GGAGAGATTC AGTCAAAAGG ACCTAAACAG TAAAATCATT 
CTTTATGTCA TCAATCCATC TTCAGAAATG AATTCGGATA ATTTGGAGTT TCAAGTCACA 
GACCCCAGTG GAAACACCGC TGCCCCTCAA ATTTTGGAAC TAAAGTGGTC CCACATTCAT 
TTGCCCCAGA CTGAATATGA AGTTTGTGAA AACATGAACA CAGTACCACT GAAAATCACT 
AGGATGGGCC ATTTAATGGA ATCAGCCTTT GTTGGAGTAA AGGTTAATCA AGTATCTGCT 
ACAATTGGAA AAGATTTCAC CGTGAGTCCA TCAAAGCTGA TTCAGTTTGA TCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTCAACTC CCCTGTGAAT GCCGTTCTTG GCACGAAGAC AAAAGCCAGA 
GTGAAAATTT TGGACTCAAA AGGAGGACAG TGCCTCCATT CACATTCCTC TGGCCAAAAC 
CAGTACAACT CATGGGGGAA AGGCATATGG CTCCCACTTT CCCCAGGCTC ATCATCACCC 
TCCATTTCTG ATGGGATTCA TTTGGAAGGA AGACACCCTT CATCTTCCAA AGAGGGGATA 
ACACACAGCA GAGGGGACAA GCTTCGGGGC TTTGACTTAA CTGATATTCC CAGGAAAAGG 
CTTAGGACAA TGGGAAATGG CAAAACAGTT CACCCATCAT CCATTTATAG AAATGAAACA 
GATATAATTT TTAGGTATCA TGGTATGGTT GGATTAAGAG TAGAAGATGA CAAATCTGCC 
ATAAATAGAG AGAAGACCAG AGGGTCTATA ACTAATCAGA GGCTACAAAA GAAAAGCAGG 
AACACAGAAC CACTATGGGC AGATAAGGTG GACCAGATGC CCTATTTACA CTCCCCTATT 
CAGGACCAGT CACCATCCTT TCCAAAAAAT TGCACATTAG AATTAAAGGG ACTCTTTCAT 
TTTGAGGAAA GCTTCCAAAA ATTATATCAT TGCAATGGAA CTTCTTGGAA GCCTTGGAGT 
CCAAAAGACC AGGATGGGAA TGAAAGAACC TGTCCCATTG GATGGAGCCA TCAAAGCAGC 
CATTGCTACT CATTGATTAC AGAGCAAAAG GTCACCTGGA ATATGGCTGC TCGGGCTTGC 
AGGGAACAAC ATCAAGGCAC CCTAGCAAGT GTGTTTACGA AACAGCATAT GCAGTGGCTA 
TGGGACTTCA GTGGAAGAAA ATCCTTCTGG ATAGGTTTGA ATGACCAAGA GAATGCAGAT 
CGCTGGGAAT GGGATGGAGG TGAACCTGTA ACCTTCACAA ACTGGAAGAG AGAGGCCCCC 
CCACATTTAA AGAAAAGAAA GAATTGCGTT TTGGTAAAAA GAAGAGGCAA GTGGCAAGCT 
AAAGACTGCA GGAAGGATAA AGCTCACAAC TACATGTGTG CCAGGAAACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007499645.1
CDS	88..2241
Translation	MVKQASMLKATLPLFARFVLSNGLKTKHGVFKIKLESIDQALPVVTQNKGLRIMERTMALLSPDILQLSDPDTPTENLTFLLAQLPQYGQLYLRGMVLTQHNFTQQEVDSKNVAYQHLGGNSQVDQFTFVATDGTNQGFVVDGRVQNEPVSFIIQVSQLDKKAPRIIQLQCPSQVELLKNGCYGIYITSRVLKASDPDTEDNQIIFKILRGPQYGHLENTTTGEFIQERFSQKDLNSKIILYVINPSSEMNSDNLEFQVTDPSGNTAAPQILELKWSHIHLPQTEYEVCENMNTVPLKITRMGHLMESAFVGVKVNQVSATIGKDFTVSPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKARVKILDSKGGQCLHSHSSGQNQYNSWGKGIWLPLSPGSSSPSISDGIHLEGRHPSSSKEGITHSRGDKLRGFDLTDIPRKRLRTMGNGKTVHPSSIYRNETDIIFRYHGMVGLRVEDDKSAINREKTRGSITNQRLQKKSRNTEPLWADKVDQMPYLHSPIQDQSPSFPKNCTLELKGLFHFEESFQKLYHCNGTSWKPWSPKDQDGNERTCPIGWSHQSSHCYSLITEQKVTWNMAARACREQHQGTLASVFTKQHMQWLWDFSGRKSFWIGLNDQENADRWEWDGGEPVTFTNWKREAPPHLKKRKNCVLVKRRGKWQAKDCRKDKAHNYMCARKL

Target ORF information:

RefSeq Version	XM_007499583.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007499583.2

ORF Insert Sequence:

ATGGTGAAAC AAGCATCCAT GCTGAAGGCT ACCTTGCCTC TCTTTGCCAG ATTCGTCTTG 
AGCAATGGAC TAAAGACAAA GCATGGAGTG TTTAAGATCA AACTGGAGTC TATTGACCAA 
GCCTTGCCTG TTGTGACCCA GAACAAGGGG TTAAGAATAA TGGAAAGGAC CATGGCGCTC 
CTCTCTCCAG ACATCCTTCA ACTTTCTGAC CCAGACACTC CAACAGAAAA CCTCACCTTC 
CTCTTGGCCC AGCTCCCCCA GTATGGACAG CTCTATCTTC GGGGGATGGT TCTCACACAA 
CACAACTTTA CACAACAAGA AGTGGACAGT AAGAATGTGG CCTACCAACA CCTAGGAGGA 
AACTCACAGG TTGACCAGTT TACCTTTGTA GCTACTGATG GGACAAATCA AGGCTTTGTT 
GTGGACGGAA GAGTGCAGAA TGAACCTGTG TCTTTCATTA TTCAGGTGTC CCAGTTGGAT 
AAAAAAGCAC CACGCATCAT CCAGCTTCAG TGCCCTTCCC AAGTGGAGCT TCTGAAAAAT 
GGTTGCTATG GGATTTACAT AACATCTCGA GTCCTGAAGG CATCTGACCC TGATACAGAG 
GACAACCAAA TCATCTTTAA GATTTTACGA GGCCCCCAGT ACGGACATCT GGAAAATACA 
ACAACAGGTG AATTTATTCA GGAGAGATTC AGTCAAAAGG ACCTAAACAG TAAAATCATT 
CTTTATGTCA TCAATCCATC TTCAGAAATG AATTCGGATA ATTTGGAGTT TCAAGTCACA 
GACCCCAGTG GAAACACCGC TGCCCCTCAA ATTTTGGAAC TAAAGTGGTC CCACATTCAT 
TTGCCCCAGA CTGAATATGA AGTTTGTGAA AACATGAACA CAGTACCACT GAAAATCACT 
AGGATGGGCC ATTTAATGGA ATCAGCCTTT GTTGGAGTAA AGGTTAATCA AGTATCTGCT 
ACAATTGGAA AAGATTTCAC CGTGAGTCCA TCAAAGCTGA TTCAGTTTGA TCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTCAACTC CCCTGTGAAT GCCGTTCTTG GCACGAAGAC AAAAGCCAGA 
GTGAAAATTT TGGACTCAAA AGGAGGACAG TGCCTCCATT CACATTCCTC TGGCCAAAAC 
CAGTACAACT CATGGGGGAA AGGCATATGG CTCCCACTTT CCCCAGGCTC ATCATCACCC 
TCCATTTCTG ATGGGATTCA TTTGGAAGGA AGACACCCTT CATCTTCCAA AGAGGGGATA 
ACACACAGCA GAGGGGACAA GCTTCGGGGC TTTGACTTAA CTGATATTCC CAGGAAAAGG 
CTTAGGACAA TGGGAAATGG CAAAACAGTT CACCCATCAT CCATTTATAG AAATGAAACA 
GATATAATTT TTAGGTATCA TGGTATGGTT GGATTAAGAG TAGAAGATGA CAAATCTGCC 
ATAAATAGAG AGAAGACCAG AGGGTCTATA ACTAATCAGA GGCTACAAAA GAAAAGCAGG 
AACACAGAAC CACTATGGGC AGATAAGGTG GACCAGATGC CCTATTTACA CTCCCCTATT 
CAGGACCAGT CACCATCCTT TCCAAAAAAT TGCACATTAG AATTAAAGGG ACTCTTTCAT 
TTTGAGGAAA GCTTCCAAAA ATTATATCAT TGCAATGGAA CTTCTTGGAA GCCTTGGAGT 
CCAAAAGACC AGGATGGGAA TGAAAGAACC TGTCCCATTG GATGGAGCCA TCAAAGCAGC 
CATTGCTACT CATTGATTAC AGAGCAAAAG GTCACCTGGA ATATGGCTGC TCGGGCTTGC 
AGGGAACAAC ATCAAGGCAC CCTAGCAAGT GTGTTTACGA AACAGCATAT GCAGTGGCTA 
TGGGACTTCA GTGGAAGAAA ATCCTTCTGG ATAGGTTTGA ATGACCAAGA GAATGCAGAT 
CGCTGGGAAT GGGATGGAGG TGAACCTGTA ACCTTCACAA ACTGGAAGAG AGAGGCCCCC 
CCACATTTAA AGAAAAGAAA GAATTGCGTT TTGGTAAAAA GAAGAGGCAA GTGGCAAGCT 
AAAGACTGCA GGAAGGATAA AGCTCACAAC TACATGTGTG CCAGGAAACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_007499584.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007499646.1 Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMc28696
Clone ID Related Accession (Same CDS sequence)	XM_007499582.1 , XM_007499583.2 , XM_007499584.2 , XM_016424262.1
Accession Version	XM_007499584.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007499584.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAC AAGCATCCAT GCTGAAGGCT ACCTTGCCTC TCTTTGCCAG ATTCGTCTTG 
AGCAATGGAC TAAAGACAAA GCATGGAGTG TTTAAGATCA AACTGGAGTC TATTGACCAA 
GCCTTGCCTG TTGTGACCCA GAACAAGGGG TTAAGAATAA TGGAAAGGAC CATGGCGCTC 
CTCTCTCCAG ACATCCTTCA ACTTTCTGAC CCAGACACTC CAACAGAAAA CCTCACCTTC 
CTCTTGGCCC AGCTCCCCCA GTATGGACAG CTCTATCTTC GGGGGATGGT TCTCACACAA 
CACAACTTTA CACAACAAGA AGTGGACAGT AAGAATGTGG CCTACCAACA CCTAGGAGGA 
AACTCACAGG TTGACCAGTT TACCTTTGTA GCTACTGATG GGACAAATCA AGGCTTTGTT 
GTGGACGGAA GAGTGCAGAA TGAACCTGTG TCTTTCATTA TTCAGGTGTC CCAGTTGGAT 
AAAAAAGCAC CACGCATCAT CCAGCTTCAG TGCCCTTCCC AAGTGGAGCT TCTGAAAAAT 
GGTTGCTATG GGATTTACAT AACATCTCGA GTCCTGAAGG CATCTGACCC TGATACAGAG 
GACAACCAAA TCATCTTTAA GATTTTACGA GGCCCCCAGT ACGGACATCT GGAAAATACA 
ACAACAGGTG AATTTATTCA GGAGAGATTC AGTCAAAAGG ACCTAAACAG TAAAATCATT 
CTTTATGTCA TCAATCCATC TTCAGAAATG AATTCGGATA ATTTGGAGTT TCAAGTCACA 
GACCCCAGTG GAAACACCGC TGCCCCTCAA ATTTTGGAAC TAAAGTGGTC CCACATTCAT 
TTGCCCCAGA CTGAATATGA AGTTTGTGAA AACATGAACA CAGTACCACT GAAAATCACT 
AGGATGGGCC ATTTAATGGA ATCAGCCTTT GTTGGAGTAA AGGTTAATCA AGTATCTGCT 
ACAATTGGAA AAGATTTCAC CGTGAGTCCA TCAAAGCTGA TTCAGTTTGA TCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTCAACTC CCCTGTGAAT GCCGTTCTTG GCACGAAGAC AAAAGCCAGA 
GTGAAAATTT TGGACTCAAA AGGAGGACAG TGCCTCCATT CACATTCCTC TGGCCAAAAC 
CAGTACAACT CATGGGGGAA AGGCATATGG CTCCCACTTT CCCCAGGCTC ATCATCACCC 
TCCATTTCTG ATGGGATTCA TTTGGAAGGA AGACACCCTT CATCTTCCAA AGAGGGGATA 
ACACACAGCA GAGGGGACAA GCTTCGGGGC TTTGACTTAA CTGATATTCC CAGGAAAAGG 
CTTAGGACAA TGGGAAATGG CAAAACAGTT CACCCATCAT CCATTTATAG AAATGAAACA 
GATATAATTT TTAGGTATCA TGGTATGGTT GGATTAAGAG TAGAAGATGA CAAATCTGCC 
ATAAATAGAG AGAAGACCAG AGGGTCTATA ACTAATCAGA GGCTACAAAA GAAAAGCAGG 
AACACAGAAC CACTATGGGC AGATAAGGTG GACCAGATGC CCTATTTACA CTCCCCTATT 
CAGGACCAGT CACCATCCTT TCCAAAAAAT TGCACATTAG AATTAAAGGG ACTCTTTCAT 
TTTGAGGAAA GCTTCCAAAA ATTATATCAT TGCAATGGAA CTTCTTGGAA GCCTTGGAGT 
CCAAAAGACC AGGATGGGAA TGAAAGAACC TGTCCCATTG GATGGAGCCA TCAAAGCAGC 
CATTGCTACT CATTGATTAC AGAGCAAAAG GTCACCTGGA ATATGGCTGC TCGGGCTTGC 
AGGGAACAAC ATCAAGGCAC CCTAGCAAGT GTGTTTACGA AACAGCATAT GCAGTGGCTA 
TGGGACTTCA GTGGAAGAAA ATCCTTCTGG ATAGGTTTGA ATGACCAAGA GAATGCAGAT 
CGCTGGGAAT GGGATGGAGG TGAACCTGTA ACCTTCACAA ACTGGAAGAG AGAGGCCCCC 
CCACATTTAA AGAAAAGAAA GAATTGCGTT TTGGTAAAAA GAAGAGGCAA GTGGCAAGCT 
AAAGACTGCA GGAAGGATAA AGCTCACAAC TACATGTGTG CCAGGAAACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007499646.1
CDS	62..2215
Translation	MVKQASMLKATLPLFARFVLSNGLKTKHGVFKIKLESIDQALPVVTQNKGLRIMERTMALLSPDILQLSDPDTPTENLTFLLAQLPQYGQLYLRGMVLTQHNFTQQEVDSKNVAYQHLGGNSQVDQFTFVATDGTNQGFVVDGRVQNEPVSFIIQVSQLDKKAPRIIQLQCPSQVELLKNGCYGIYITSRVLKASDPDTEDNQIIFKILRGPQYGHLENTTTGEFIQERFSQKDLNSKIILYVINPSSEMNSDNLEFQVTDPSGNTAAPQILELKWSHIHLPQTEYEVCENMNTVPLKITRMGHLMESAFVGVKVNQVSATIGKDFTVSPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKARVKILDSKGGQCLHSHSSGQNQYNSWGKGIWLPLSPGSSSPSISDGIHLEGRHPSSSKEGITHSRGDKLRGFDLTDIPRKRLRTMGNGKTVHPSSIYRNETDIIFRYHGMVGLRVEDDKSAINREKTRGSITNQRLQKKSRNTEPLWADKVDQMPYLHSPIQDQSPSFPKNCTLELKGLFHFEESFQKLYHCNGTSWKPWSPKDQDGNERTCPIGWSHQSSHCYSLITEQKVTWNMAARACREQHQGTLASVFTKQHMQWLWDFSGRKSFWIGLNDQENADRWEWDGGEPVTFTNWKREAPPHLKKRKNCVLVKRRGKWQAKDCRKDKAHNYMCARKL

Target ORF information:

RefSeq Version	XM_007499584.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007499584.2

ORF Insert Sequence:

ATGGTGAAAC AAGCATCCAT GCTGAAGGCT ACCTTGCCTC TCTTTGCCAG ATTCGTCTTG 
AGCAATGGAC TAAAGACAAA GCATGGAGTG TTTAAGATCA AACTGGAGTC TATTGACCAA 
GCCTTGCCTG TTGTGACCCA GAACAAGGGG TTAAGAATAA TGGAAAGGAC CATGGCGCTC 
CTCTCTCCAG ACATCCTTCA ACTTTCTGAC CCAGACACTC CAACAGAAAA CCTCACCTTC 
CTCTTGGCCC AGCTCCCCCA GTATGGACAG CTCTATCTTC GGGGGATGGT TCTCACACAA 
CACAACTTTA CACAACAAGA AGTGGACAGT AAGAATGTGG CCTACCAACA CCTAGGAGGA 
AACTCACAGG TTGACCAGTT TACCTTTGTA GCTACTGATG GGACAAATCA AGGCTTTGTT 
GTGGACGGAA GAGTGCAGAA TGAACCTGTG TCTTTCATTA TTCAGGTGTC CCAGTTGGAT 
AAAAAAGCAC CACGCATCAT CCAGCTTCAG TGCCCTTCCC AAGTGGAGCT TCTGAAAAAT 
GGTTGCTATG GGATTTACAT AACATCTCGA GTCCTGAAGG CATCTGACCC TGATACAGAG 
GACAACCAAA TCATCTTTAA GATTTTACGA GGCCCCCAGT ACGGACATCT GGAAAATACA 
ACAACAGGTG AATTTATTCA GGAGAGATTC AGTCAAAAGG ACCTAAACAG TAAAATCATT 
CTTTATGTCA TCAATCCATC TTCAGAAATG AATTCGGATA ATTTGGAGTT TCAAGTCACA 
GACCCCAGTG GAAACACCGC TGCCCCTCAA ATTTTGGAAC TAAAGTGGTC CCACATTCAT 
TTGCCCCAGA CTGAATATGA AGTTTGTGAA AACATGAACA CAGTACCACT GAAAATCACT 
AGGATGGGCC ATTTAATGGA ATCAGCCTTT GTTGGAGTAA AGGTTAATCA AGTATCTGCT 
ACAATTGGAA AAGATTTCAC CGTGAGTCCA TCAAAGCTGA TTCAGTTTGA TCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTCAACTC CCCTGTGAAT GCCGTTCTTG GCACGAAGAC AAAAGCCAGA 
GTGAAAATTT TGGACTCAAA AGGAGGACAG TGCCTCCATT CACATTCCTC TGGCCAAAAC 
CAGTACAACT CATGGGGGAA AGGCATATGG CTCCCACTTT CCCCAGGCTC ATCATCACCC 
TCCATTTCTG ATGGGATTCA TTTGGAAGGA AGACACCCTT CATCTTCCAA AGAGGGGATA 
ACACACAGCA GAGGGGACAA GCTTCGGGGC TTTGACTTAA CTGATATTCC CAGGAAAAGG 
CTTAGGACAA TGGGAAATGG CAAAACAGTT CACCCATCAT CCATTTATAG AAATGAAACA 
GATATAATTT TTAGGTATCA TGGTATGGTT GGATTAAGAG TAGAAGATGA CAAATCTGCC 
ATAAATAGAG AGAAGACCAG AGGGTCTATA ACTAATCAGA GGCTACAAAA GAAAAGCAGG 
AACACAGAAC CACTATGGGC AGATAAGGTG GACCAGATGC CCTATTTACA CTCCCCTATT 
CAGGACCAGT CACCATCCTT TCCAAAAAAT TGCACATTAG AATTAAAGGG ACTCTTTCAT 
TTTGAGGAAA GCTTCCAAAA ATTATATCAT TGCAATGGAA CTTCTTGGAA GCCTTGGAGT 
CCAAAAGACC AGGATGGGAA TGAAAGAACC TGTCCCATTG GATGGAGCCA TCAAAGCAGC 
CATTGCTACT CATTGATTAC AGAGCAAAAG GTCACCTGGA ATATGGCTGC TCGGGCTTGC 
AGGGAACAAC ATCAAGGCAC CCTAGCAAGT GTGTTTACGA AACAGCATAT GCAGTGGCTA 
TGGGACTTCA GTGGAAGAAA ATCCTTCTGG ATAGGTTTGA ATGACCAAGA GAATGCAGAT 
CGCTGGGAAT GGGATGGAGG TGAACCTGTA ACCTTCACAA ACTGGAAGAG AGAGGCCCCC 
CCACATTTAA AGAAAAGAAA GAATTGCGTT TTGGTAAAAA GAAGAGGCAA GTGGCAAGCT 
AAAGACTGCA GGAAGGATAA AGCTCACAAC TACATGTGTG CCAGGAAACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_016424262.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016279748.1 Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA.

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CloneID	OMc28696
Clone ID Related Accession (Same CDS sequence)	XM_007499582.1 , XM_007499583.2 , XM_007499584.2 , XM_016424262.1
Accession Version	XM_016424262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAC AAGCATCCAT GCTGAAGGCT ACCTTGCCTC TCTTTGCCAG ATTCGTCTTG 
AGCAATGGAC TAAAGACAAA GCATGGAGTG TTTAAGATCA AACTGGAGTC TATTGACCAA 
GCCTTGCCTG TTGTGACCCA GAACAAGGGG TTAAGAATAA TGGAAAGGAC CATGGCGCTC 
CTCTCTCCAG ACATCCTTCA ACTTTCTGAC CCAGACACTC CAACAGAAAA CCTCACCTTC 
CTCTTGGCCC AGCTCCCCCA GTATGGACAG CTCTATCTTC GGGGGATGGT TCTCACACAA 
CACAACTTTA CACAACAAGA AGTGGACAGT AAGAATGTGG CCTACCAACA CCTAGGAGGA 
AACTCACAGG TTGACCAGTT TACCTTTGTA GCTACTGATG GGACAAATCA AGGCTTTGTT 
GTGGACGGAA GAGTGCAGAA TGAACCTGTG TCTTTCATTA TTCAGGTGTC CCAGTTGGAT 
AAAAAAGCAC CACGCATCAT CCAGCTTCAG TGCCCTTCCC AAGTGGAGCT TCTGAAAAAT 
GGTTGCTATG GGATTTACAT AACATCTCGA GTCCTGAAGG CATCTGACCC TGATACAGAG 
GACAACCAAA TCATCTTTAA GATTTTACGA GGCCCCCAGT ACGGACATCT GGAAAATACA 
ACAACAGGTG AATTTATTCA GGAGAGATTC AGTCAAAAGG ACCTAAACAG TAAAATCATT 
CTTTATGTCA TCAATCCATC TTCAGAAATG AATTCGGATA ATTTGGAGTT TCAAGTCACA 
GACCCCAGTG GAAACACCGC TGCCCCTCAA ATTTTGGAAC TAAAGTGGTC CCACATTCAT 
TTGCCCCAGA CTGAATATGA AGTTTGTGAA AACATGAACA CAGTACCACT GAAAATCACT 
AGGATGGGCC ATTTAATGGA ATCAGCCTTT GTTGGAGTAA AGGTTAATCA AGTATCTGCT 
ACAATTGGAA AAGATTTCAC CGTGAGTCCA TCAAAGCTGA TTCAGTTTGA TCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTCAACTC CCCTGTGAAT GCCGTTCTTG GCACGAAGAC AAAAGCCAGA 
GTGAAAATTT TGGACTCAAA AGGAGGACAG TGCCTCCATT CACATTCCTC TGGCCAAAAC 
CAGTACAACT CATGGGGGAA AGGCATATGG CTCCCACTTT CCCCAGGCTC ATCATCACCC 
TCCATTTCTG ATGGGATTCA TTTGGAAGGA AGACACCCTT CATCTTCCAA AGAGGGGATA 
ACACACAGCA GAGGGGACAA GCTTCGGGGC TTTGACTTAA CTGATATTCC CAGGAAAAGG 
CTTAGGACAA TGGGAAATGG CAAAACAGTT CACCCATCAT CCATTTATAG AAATGAAACA 
GATATAATTT TTAGGTATCA TGGTATGGTT GGATTAAGAG TAGAAGATGA CAAATCTGCC 
ATAAATAGAG AGAAGACCAG AGGGTCTATA ACTAATCAGA GGCTACAAAA GAAAAGCAGG 
AACACAGAAC CACTATGGGC AGATAAGGTG GACCAGATGC CCTATTTACA CTCCCCTATT 
CAGGACCAGT CACCATCCTT TCCAAAAAAT TGCACATTAG AATTAAAGGG ACTCTTTCAT 
TTTGAGGAAA GCTTCCAAAA ATTATATCAT TGCAATGGAA CTTCTTGGAA GCCTTGGAGT 
CCAAAAGACC AGGATGGGAA TGAAAGAACC TGTCCCATTG GATGGAGCCA TCAAAGCAGC 
CATTGCTACT CATTGATTAC AGAGCAAAAG GTCACCTGGA ATATGGCTGC TCGGGCTTGC 
AGGGAACAAC ATCAAGGCAC CCTAGCAAGT GTGTTTACGA AACAGCATAT GCAGTGGCTA 
TGGGACTTCA GTGGAAGAAA ATCCTTCTGG ATAGGTTTGA ATGACCAAGA GAATGCAGAT 
CGCTGGGAAT GGGATGGAGG TGAACCTGTA ACCTTCACAA ACTGGAAGAG AGAGGCCCCC 
CCACATTTAA AGAAAAGAAA GAATTGCGTT TTGGTAAAAA GAAGAGGCAA GTGGCAAGCT 
AAAGACTGCA GGAAGGATAA AGCTCACAAC TACATGTGTG CCAGGAAACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016279748.1
CDS	22..2175
Translation	MVKQASMLKATLPLFARFVLSNGLKTKHGVFKIKLESIDQALPVVTQNKGLRIMERTMALLSPDILQLSDPDTPTENLTFLLAQLPQYGQLYLRGMVLTQHNFTQQEVDSKNVAYQHLGGNSQVDQFTFVATDGTNQGFVVDGRVQNEPVSFIIQVSQLDKKAPRIIQLQCPSQVELLKNGCYGIYITSRVLKASDPDTEDNQIIFKILRGPQYGHLENTTTGEFIQERFSQKDLNSKIILYVINPSSEMNSDNLEFQVTDPSGNTAAPQILELKWSHIHLPQTEYEVCENMNTVPLKITRMGHLMESAFVGVKVNQVSATIGKDFTVSPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKARVKILDSKGGQCLHSHSSGQNQYNSWGKGIWLPLSPGSSSPSISDGIHLEGRHPSSSKEGITHSRGDKLRGFDLTDIPRKRLRTMGNGKTVHPSSIYRNETDIIFRYHGMVGLRVEDDKSAINREKTRGSITNQRLQKKSRNTEPLWADKVDQMPYLHSPIQDQSPSFPKNCTLELKGLFHFEESFQKLYHCNGTSWKPWSPKDQDGNERTCPIGWSHQSSHCYSLITEQKVTWNMAARACREQHQGTLASVFTKQHMQWLWDFSGRKSFWIGLNDQENADRWEWDGGEPVTFTNWKREAPPHLKKRKNCVLVKRRGKWQAKDCRKDKAHNYMCARKL

Target ORF information:

RefSeq Version	XM_016424262.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016424262.1

ORF Insert Sequence:

ATGGTGAAAC AAGCATCCAT GCTGAAGGCT ACCTTGCCTC TCTTTGCCAG ATTCGTCTTG 
AGCAATGGAC TAAAGACAAA GCATGGAGTG TTTAAGATCA AACTGGAGTC TATTGACCAA 
GCCTTGCCTG TTGTGACCCA GAACAAGGGG TTAAGAATAA TGGAAAGGAC CATGGCGCTC 
CTCTCTCCAG ACATCCTTCA ACTTTCTGAC CCAGACACTC CAACAGAAAA CCTCACCTTC 
CTCTTGGCCC AGCTCCCCCA GTATGGACAG CTCTATCTTC GGGGGATGGT TCTCACACAA 
CACAACTTTA CACAACAAGA AGTGGACAGT AAGAATGTGG CCTACCAACA CCTAGGAGGA 
AACTCACAGG TTGACCAGTT TACCTTTGTA GCTACTGATG GGACAAATCA AGGCTTTGTT 
GTGGACGGAA GAGTGCAGAA TGAACCTGTG TCTTTCATTA TTCAGGTGTC CCAGTTGGAT 
AAAAAAGCAC CACGCATCAT CCAGCTTCAG TGCCCTTCCC AAGTGGAGCT TCTGAAAAAT 
GGTTGCTATG GGATTTACAT AACATCTCGA GTCCTGAAGG CATCTGACCC TGATACAGAG 
GACAACCAAA TCATCTTTAA GATTTTACGA GGCCCCCAGT ACGGACATCT GGAAAATACA 
ACAACAGGTG AATTTATTCA GGAGAGATTC AGTCAAAAGG ACCTAAACAG TAAAATCATT 
CTTTATGTCA TCAATCCATC TTCAGAAATG AATTCGGATA ATTTGGAGTT TCAAGTCACA 
GACCCCAGTG GAAACACCGC TGCCCCTCAA ATTTTGGAAC TAAAGTGGTC CCACATTCAT 
TTGCCCCAGA CTGAATATGA AGTTTGTGAA AACATGAACA CAGTACCACT GAAAATCACT 
AGGATGGGCC ATTTAATGGA ATCAGCCTTT GTTGGAGTAA AGGTTAATCA AGTATCTGCT 
ACAATTGGAA AAGATTTCAC CGTGAGTCCA TCAAAGCTGA TTCAGTTTGA TCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTCAACTC CCCTGTGAAT GCCGTTCTTG GCACGAAGAC AAAAGCCAGA 
GTGAAAATTT TGGACTCAAA AGGAGGACAG TGCCTCCATT CACATTCCTC TGGCCAAAAC 
CAGTACAACT CATGGGGGAA AGGCATATGG CTCCCACTTT CCCCAGGCTC ATCATCACCC 
TCCATTTCTG ATGGGATTCA TTTGGAAGGA AGACACCCTT CATCTTCCAA AGAGGGGATA 
ACACACAGCA GAGGGGACAA GCTTCGGGGC TTTGACTTAA CTGATATTCC CAGGAAAAGG 
CTTAGGACAA TGGGAAATGG CAAAACAGTT CACCCATCAT CCATTTATAG AAATGAAACA 
GATATAATTT TTAGGTATCA TGGTATGGTT GGATTAAGAG TAGAAGATGA CAAATCTGCC 
ATAAATAGAG AGAAGACCAG AGGGTCTATA ACTAATCAGA GGCTACAAAA GAAAAGCAGG 
AACACAGAAC CACTATGGGC AGATAAGGTG GACCAGATGC CCTATTTACA CTCCCCTATT 
CAGGACCAGT CACCATCCTT TCCAAAAAAT TGCACATTAG AATTAAAGGG ACTCTTTCAT 
TTTGAGGAAA GCTTCCAAAA ATTATATCAT TGCAATGGAA CTTCTTGGAA GCCTTGGAGT 
CCAAAAGACC AGGATGGGAA TGAAAGAACC TGTCCCATTG GATGGAGCCA TCAAAGCAGC 
CATTGCTACT CATTGATTAC AGAGCAAAAG GTCACCTGGA ATATGGCTGC TCGGGCTTGC 
AGGGAACAAC ATCAAGGCAC CCTAGCAAGT GTGTTTACGA AACAGCATAT GCAGTGGCTA 
TGGGACTTCA GTGGAAGAAA ATCCTTCTGG ATAGGTTTGA ATGACCAAGA GAATGCAGAT 
CGCTGGGAAT GGGATGGAGG TGAACCTGTA ACCTTCACAA ACTGGAAGAG AGAGGCCCCC 
CCACATTTAA AGAAAAGAAA GAATTGCGTT TTGGTAAAAA GAAGAGGCAA GTGGCAAGCT 
AAAGACTGCA GGAAGGATAA AGCTCACAAC TACATGTGTG CCAGGAAACT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_007499581.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007499643.1 Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMc28695
Clone ID Related Accession (Same CDS sequence)	XM_007499581.2 , XM_001372782.3
Accession Version	XM_007499581.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007499581.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGC TTGGATCCAG TGGGGTGTTT CTCAGCCTGC TGCTTGTCCT GCAAAGTACC 
AACTCATCCT TCATCAGCGT CAACCAGGGC TTGAAGGTGG TGAAGGGCCA CTCTGCTTTC 
CTGTCAAGGG AGGACCTGCA GTTTTCCATT CCCAAAGAAA AAGATGCCTG CAAAGTGGAA 
GTTGTGCTAA ATGAACCAAT AACTCAGAGG GTTGGGAAAC TCACTCCACA GGTATTTGAC 
TGTCATTTCC TTCCTAATGA AGTAAAATAC ATTCATAATG GGTGCCCAAT CCTACATGAA 
GATGCTGTTA AACTTAGGCT CTACAGATTT ACAGAAACAG AAACCTTCAC AGAAACTTTT 
ATCCTTCGGG TTTATCTGCA GGAGCCAGAC TGTAGCATCA TCCGGATGAG CTCCAGGGTC 
CTGGAGGTGC CTGAGTTCTA TGGCCTCTCC AGAGCAATTG ATAAGAATGT GCTCAGTTTT 
GATTATGAGA GGAGGATGAG TCTGGAATGC ACCATAAGAC TCTCTCCATT AGAAACTGGC 
CTACCAGCCC ATGGTCAGCT GGTCATTGTG GAACCTCAGC AAGGAGAACC CCGAGGAGAC 
CAGCCCCACA GCTTTTTCCC TGAGTCTCAA TATAGGAAAA ATCCAGGATG CCCGAGTGGA 
AGCTGTTCCC CAGAGTTGAA GAAGATTGAT AGTCTCAAAG TCAGCTGTGA GGAGTTTCTG 
CTAATGGGGT TGCGATATCA GCACCTCCAT CCACCTTCTC CGAATGTCGA TTATATCTCC 
ATCAGACTGG ACCTCACTGA TATCAGGAGT AAATCCATAT ACAAGTCAGA GAGCATGTGG 
CTGCCTGTCT CCATCAAAGG TGCCTTCCCC AATCAGATCC CCAGGGCTGC ATTCATGCCC 
ACCTATATTC TAGAAGTGGA TCAATTCATC CTGAGCTCAG TGACCACTTC TACAGTGGAC 
TGCGAAGATG ATGAGACACC TAGATCTCTG TTGGTTTTCA ACATCACCAA GCCCCCTCTC 
CAGGGCTACT TCACACACCT ATTGGATCAC ACCAAAGAGA TCTCTTCATT CACCTGGAAA 
GATCTAAGTG ATTTGCAAAT TGCCTATCAG CCTCCCAACA GCAGCCACAC AGAGAGAAGG 
AATTATGAGG TGGAGTTTGA AGTGTACGAC TTCTTCTTTG AAAAGAGCTT ACCAATCACA 
GTCCATATTT CTATCAGGAC AGCAGACACG AATGCCCCAA GAGTGTCATG GAACACAGGT 
CTCAGTCTTC TAGAGGGACA GTCAAGGCCC ATCACCTGGG AGCAGTTTCA GATAGTGGAC 
AATGATGACA TCAAGGCAGT TCGCCTGGTC ACTGTTGATG GCTTACAGCA TGGGAGACTT 
ACTGTAAGAG GAGGGAAAGG CTTCATTTTC TCTGTAGCCG ACATTCAGGC TGGGACTGTT 
CGTTACCATC ATGATGACAG TGACTCCACC AAGGATTTTG TGGTCTTCAG GATTTTTGAT 
GGCCATCACA GCACTCGCCA CAAGTTTCCC ATCAATATCC TGCCTAAAGA TGACAGCCCG 
CCTCTCCTCA TAACAAATGT TGGGATTGAA TTAGACGAGG GGCAGACTGT GCTGATCCAA 
GGCTCCATGC TGAAAGCTTC TGACACGGAC TCCAGTGATG ACTACATTTT CTTTAATATC 
ACTAAACCTT CCCAGGCTGG AGAAATCATT AAGAAGCCAG GTCCAGGACT TATTGGTTAC 
CCAGTCCCTG GTTTTCTTCA GAGAGATCTG TTTAATGGGA TTATCTATTA TCGACACTTT 
GGTGGAGAAA CTTTTGAGGA TTCCTTTGAG CTTGTTCTTT GTGACAGCCA CGATCCTCCC 
AACCTCTCAG AACCACAGGT AGTGGTGATA CACATCACCC CAGTGGATGA TGAACTTCCA 
AAAGAGGTTC CTGGAGCGAT TCGGCATTTG GTTGTCAAGG AAACAGAGGT GGCTCATCTC 
ACTAAGAAGC ATCTACATTT TGTGGATACA GAATCTCAGG ATAGGGAGCT TCTCTACACA 
ATAACAAGAC CTCCATTTTC CTCCTTCAGA TATGACCATT CTGATGCTGG GAAATTGTTC 
ATGGTGGATA GCATATCGAA GCTAATCAAG GATCCTGCAG CTCTGGGACT GAGATCATTC 
ACCCAGCATG CTGTAAACTA CATGAAGGTG GCTTACATGC CACCCATGCA AGACATTGGA 
CCCTATGTTC AGCATGTTCA GTTTGTTTTT TCCATCAGTA ACCAGCATGG CGGCACTTTG 
CATGGAATTT GCTTTAATAT TACTATTCTA CCAGTGGATA ATGAGGCTCC TGAGGTGTTC 
ACCAACTCCC TGCAAGTAGA AGAAGGAGGT CTATGTATCA TCACAACAGA ACACATTCTG 
ATTTCAGATA TGGATACAAT ACAAGATAAA ATCAGACTGT CCCTCCAGAG AATGCCACTC 
CATGGAATGG TTGAACTGAA TGGTTTTCCT CTAAGCCAAG GAGACAAATT TTCATGGGGA 
GACCTCCATG CATTAAAAGT TAGGTATCAC CATGATGGAT CAGAGGTTTT TCATGACAAC 
ATACTCTTTG AGGCAACAGA TGGCACAAAT TCTGCAGAAT TTGTCCTTCA GGTCAAGGTT 
TTACCTGTAA ATGATGAACC CCCAGTCCTG AAGGCTGATC TCATTCCCAC ACTGCATTGT 
CCTGAGGGAG AGGAAGTGGT CATCTCTTCA GAACATGTTT ATGCCACTGA CATGGACAGC 
GATGACACCA AACTGATGTT CATGATTGTA CGTCAGCCTC ATCATGGGGT CGTGAGGAAA 
TCTGGGATCA CCGTGGACCA GTTCACCCAG GGAGACATTG ATGCTGGAAC TGTCACATAC 
AAACACACAA GTGGAGAGAT TGGCATGGCC CCTTGTTTTG ACACCATTTC TTTGGTGGTT 
TCGGATGGAG AAACTGGTCC TCAGGACTGT TGCTACCAAG ATCCCATTTC TTCACCACTT 
CCACCTCATC ATTCTTTTCC AGTGTATGAT CTCAATGTGA CTGTGTATCC TGTGGACAAC 
CAAAGACCAA CTATTACAAC TGGTACCCAA TTTGTTGTGG ATGAGGGTTC TTCAGCTGCC 
ATTAATGTTA ACCATTTAAT CACTACTGAC CCCGACACAG CCATGGATGA CTTACAGTTT 
GCTTTGGTTT CTCCTCCACA ATTTGGTTAT ATTGAAAACA TACTTCCTTC TCCAGGTTTT 
GAGAAAAGCA ATATGGGCAT AAGCATAGCT TCATTTTGGT GGAAAGACAT GAAGGCTTTG 
CATATAAACT ATGTGCAGTC CAGACATCTG AGGATAGAGC CAACAGCAGA TCACTTTATG 
ATTTACGTCA CAGATGGAAA ACAGAATTCT ATGGAAATCC CTTTTTCCAT CCTGATTAAC 
CCCACAAATG ATGAAGTTCC TGACTTTGTG ACACAGAACA TCACTGTTTC TGAGGGTCAG 
ATGAAAGAAC TGGATCTTTC AGTCATCAAA GCAACTGACC TAGACATGCC AAAAGATCAT 
TTGATGTTCA CCATTACCCA TAAACCACAG CATGGCCTCC TCATCAATGG GATACCTAGA 
AATGACATCC CTCATTATAA GCAATTAATC AATTCTCACC AGAACCATGA ACTTCCTGTG 
TGTGATTTTT CAATGGAGCT TCTTGAAACT GGAATGAAGT TGACATATAT ACACGATGAC 
TCTGAAAGCC TTGCTGATGA CTTTACAATC CAGTTGTCAG ATGGCAAACA TAAAGTCTTA 
AGAACAATTT CCATAAAGAT CCTGCCAGTT AATGATGAGA AACCAGTGCT GACCAAGAAG 
GCTGAAATAG AAATGAATAT GGGTGAAACT CGCATTATTT CTAGTGCTAT TCTATCAGCT 
ATGGATAAAG ACACACCCAG AGAGAAAATT TACTATTTAT TTGAAAGACT TCCAGAAAAT 
GGGCAGCTTC AGCTCAAGAT AGGGAGGAAT TGGGTATCTC TTCAACCTGG CATGAAATGT 
ACTCAAGAAG AAGTAGATCT GAATTTCATG AGATACATCC ATACTGGTGC AAAAGGCTCC 
CAGGACCGAG ATAGCTTCAC ATTTCATCTC TGGGATGGAG ACAACAGGTC ACCTACGCTG 
GACTGTCATA TCAGCATCAA GGACATCAAA AAGGGTGATA TTGTGGTCTT GACCAGACCA 
TTGGTAGTGT CTAAAGGGGG CAAAGGTTTC TTGACTACAT CTACTCTCTT AGCTACTGAT 
GGAACAGATA AGCCAGAAGA ACTTCTTTTT GTAATCACCT CCCCACCTCA ATATGGCCAG 
ATGGAATACA TCACTTATCC TGGAGTCCCC ATCACAAGCT TCAATCAGAT GGATATTGCA 
GGTCAGACCG TCTGTTATGT ACACAAGAGC AAGGCAACTG GTCCCAGTGA TATGTTCAGA 
TTCGTCTTGA GCAATGGACT AAAGACAAAG CATGGAGTGT TTAAGATCAA ACTGGAGTCT 
ATTGACCAAG CCTTGCCTGT TGTGACCCAG AACAAGGGGT TAAGAATAAT GGAAAGGACC 
ATGGCGCTCC TCTCTCCAGA CATCCTTCAA CTTTCTGACC CAGACACTCC AACAGAAAAC 
CTCACCTTCC TCTTGGCCCA GCTCCCCCAG TATGGACAGC TCTATCTTCG GGGGATGGTT 
CTCACACAAC ACAACTTTAC ACAACAAGAA GTGGACAGTA AGAATGTGGC CTACCAACAC 
CTAGGAGGAA ACTCACAGGT TGACCAGTTT ACCTTTGTAG CTACTGATGG GACAAATCAA 
GGCTTTGTTG TGGACGGAAG AGTGCAGAAT GAACCTGTGT CTTTCATTAT TCAGGTGTCC 
CAGTTGGATA AAAAAGCACC ACGCATCATC CAGCTTCAGT GCCCTTCCCA AGTGGAGCTT 
CTGAAAAATG GTTGCTATGG GATTTACATA ACATCTCGAG TCCTGAAGGC ATCTGACCCT 
GATACAGAGG ACAACCAAAT CATCTTTAAG ATTTTACGAG GCCCCCAGTA CGGACATCTG 
GAAAATACAA CAACAGGTGA ATTTATTCAG GAGAGATTCA GTCAAAAGGA CCTAAACAGT 
AAAATCATTC TTTATGTCAT CAATCCATCT TCAGAAATGA ATTCGGATAA TTTGGAGTTT 
CAAGTCACAG ACCCCAGTGG AAACACCGCT GCCCCTCAAA TTTTGGAACT AAAGTGGTCC 
CACATTCATT TGCCCCAGAC TGAATATGAA GTTTGTGAAA ACATGAACAC AGTACCACTG 
AAAATCACTA GGATGGGCCA TTTAATGGAA TCAGCCTTTG TTGGAGTAAA GGTTAATCAA 
GTATCTGCTA CAATTGGAAA AGATTTCACC GTGAGTCCAT CAAAGCTGAT TCAGTTTGAT 
CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT 
GATGAGGTCT TTGAAGTAAT TCTCAACTCC CCTGTGAATG CCGTTCTTGG CACGAAGACA 
AAAGCCAGAG TGAAAATTTT GGACTCAAAA GGAGGACAGT GCCTCCATTC ACATTCCTCT 
GGCCAAAACC AGTACAACTC ATGGGGGAAA GGCATATGGC TCCCACTTTC CCCAGGCTCA 
TCATCACCCT CCATTTCTGA TGGGATTCAT TTGGAAGGAA GACACCCTTC ATCTTCCAAA 
GAGGGGATAA CACACAGCAG AGGGGACAAG CTTCGGGGCT TTGACTTAAC TGATATTCCC 
AGGAAAAGGC TTAGGACAAT GGGAAATGGC AAAACAGTTC ACCCATCATC CATTTATAGA 
AATGAAACAG ATATAATTTT TAGGTATCAT GGTATGGTTG GATTAAGAGT AGAAGATGAC 
AAATCTGCCA TAAATAGAGA GAAGACCAGA GGGTCTATAA CTAATCAGAG GCTACAAAAG 
AAAAGCAGGA ACACAGAACC ACTATGGGCA GATAAGGTGG ACCAGATGCC CTATTTACAC 
TCCCCTATTC AGGACCAGTC ACCATCCTTT CCAAAAAATT GCACATTAGA ATTAAAGGGA 
CTCTTTCATT TTGAGGAAAG CTTCCAAAAA TTATATCATT GCAATGGAAC TTCTTGGAAG 
CCTTGGAGTC CAAAAGACCA GGATGGGAAT GAAAGAACCT GTCCCATTGG ATGGAGCCAT 
CAAAGCAGCC ATTGCTACTC ATTGATTACA GAGCAAAAGG TCACCTGGAA TATGGCTGCT 
CGGGCTTGCA GGGAACAACA TCAAGGCACC CTAGCAAGTG TGTTTACGAA ACAGCATATG 
CAGTGGCTAT GGGACTTCAG TGGAAGAAAA TCCTTCTGGA TAGGTTTGAA TGACCAAGAG 
AATGCAGATC GCTGGGAATG GGATGGAGGT GAACCTGTAA CCTTCACAAA CTGGAAGAGA 
GAGGCCCCCC CACATTTAAA GAAAAGAAAG AATTGCGTTT TGGTAAAAAG AAGAGGCAAG 
TGGCAAGCTA AAGACTGCAG GAAGGATAAA GCTCACAACT ACATGTGTGC CAGGAAACTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007499643.1
CDS	821..7363
Translation	MNLLGSSGVFLSLLLVLQSTNSSFISVNQGLKVVKGHSAFLSREDLQFSIPKEKDACKVEVVLNEPITQRVGKLTPQVFDCHFLPNEVKYIHNGCPILHEDAVKLRLYRFTETETFTETFILRVYLQEPDCSIIRMSSRVLEVPEFYGLSRAIDKNVLSFDYERRMSLECTIRLSPLETGLPAHGQLVIVEPQQGEPRGDQPHSFFPESQYRKNPGCPSGSCSPELKKIDSLKVSCEEFLLMGLRYQHLHPPSPNVDYISIRLDLTDIRSKSIYKSESMWLPVSIKGAFPNQIPRAAFMPTYILEVDQFILSSVTTSTVDCEDDETPRSLLVFNITKPPLQGYFTHLLDHTKEISSFTWKDLSDLQIAYQPPNSSHTERRNYEVEFEVYDFFFEKSLPITVHISIRTADTNAPRVSWNTGLSLLEGQSRPITWEQFQIVDNDDIKAVRLVTVDGLQHGRLTVRGGKGFIFSVADIQAGTVRYHHDDSDSTKDFVVFRIFDGHHSTRHKFPINILPKDDSPPLLITNVGIELDEGQTVLIQGSMLKASDTDSSDDYIFFNITKPSQAGEIIKKPGPGLIGYPVPGFLQRDLFNGIIYYRHFGGETFEDSFELVLCDSHDPPNLSEPQVVVIHITPVDDELPKEVPGAIRHLVVKETEVAHLTKKHLHFVDTESQDRELLYTITRPPFSSFRYDHSDAGKLFMVDSISKLIKDPAALGLRSFTQHAVNYMKVAYMPPMQDIGPYVQHVQFVFSISNQHGGTLHGICFNITILPVDNEAPEVFTNSLQVEEGGLCIITTEHILISDMDTIQDKIRLSLQRMPLHGMVELNGFPLSQGDKFSWGDLHALKVRYHHDGSEVFHDNILFEATDGTNSAEFVLQVKVLPVNDEPPVLKADLIPTLHCPEGEEVVISSEHVYATDMDSDDTKLMFMIVRQPHHGVVRKSGITVDQFTQGDIDAGTVTYKHTSGEIGMAPCFDTISLVVSDGETGPQDCCYQDPISSPLPPHHSFPVYDLNVTVYPVDNQRPTITTGTQFVVDEGSSAAINVNHLITTDPDTAMDDLQFALVSPPQFGYIENILPSPGFEKSNMGISIASFWWKDMKALHINYVQSRHLRIEPTADHFMIYVTDGKQNSMEIPFSILINPTNDEVPDFVTQNITVSEGQMKELDLSVIKATDLDMPKDHLMFTITHKPQHGLLINGIPRNDIPHYKQLINSHQNHELPVCDFSMELLETGMKLTYIHDDSESLADDFTIQLSDGKHKVLRTISIKILPVNDEKPVLTKKAEIEMNMGETRIISSAILSAMDKDTPREKIYYLFERLPENGQLQLKIGRNWVSLQPGMKCTQEEVDLNFMRYIHTGAKGSQDRDSFTFHLWDGDNRSPTLDCHISIKDIKKGDIVVLTRPLVVSKGGKGFLTTSTLLATDGTDKPEELLFVITSPPQYGQMEYITYPGVPITSFNQMDIAGQTVCYVHKSKATGPSDMFRFVLSNGLKTKHGVFKIKLESIDQALPVVTQNKGLRIMERTMALLSPDILQLSDPDTPTENLTFLLAQLPQYGQLYLRGMVLTQHNFTQQEVDSKNVAYQHLGGNSQVDQFTFVATDGTNQGFVVDGRVQNEPVSFIIQVSQLDKKAPRIIQLQCPSQVELLKNGCYGIYITSRVLKASDPDTEDNQIIFKILRGPQYGHLENTTTGEFIQERFSQKDLNSKIILYVINPSSEMNSDNLEFQVTDPSGNTAAPQILELKWSHIHLPQTEYEVCENMNTVPLKITRMGHLMESAFVGVKVNQVSATIGKDFTVSPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKARVKILDSKGGQCLHSHSSGQNQYNSWGKGIWLPLSPGSSSPSISDGIHLEGRHPSSSKEGITHSRGDKLRGFDLTDIPRKRLRTMGNGKTVHPSSIYRNETDIIFRYHGMVGLRVEDDKSAINREKTRGSITNQRLQKKSRNTEPLWADKVDQMPYLHSPIQDQSPSFPKNCTLELKGLFHFEESFQKLYHCNGTSWKPWSPKDQDGNERTCPIGWSHQSSHCYSLITEQKVTWNMAARACREQHQGTLASVFTKQHMQWLWDFSGRKSFWIGLNDQENADRWEWDGGEPVTFTNWKREAPPHLKKRKNCVLVKRRGKWQAKDCRKDKAHNYMCARKL

Target ORF information:

RefSeq Version	XM_007499581.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007499581.2

ORF Insert Sequence:

ATGAACCTGC TTGGATCCAG TGGGGTGTTT CTCAGCCTGC TGCTTGTCCT GCAAAGTACC 
AACTCATCCT TCATCAGCGT CAACCAGGGC TTGAAGGTGG TGAAGGGCCA CTCTGCTTTC 
CTGTCAAGGG AGGACCTGCA GTTTTCCATT CCCAAAGAAA AAGATGCCTG CAAAGTGGAA 
GTTGTGCTAA ATGAACCAAT AACTCAGAGG GTTGGGAAAC TCACTCCACA GGTATTTGAC 
TGTCATTTCC TTCCTAATGA AGTAAAATAC ATTCATAATG GGTGCCCAAT CCTACATGAA 
GATGCTGTTA AACTTAGGCT CTACAGATTT ACAGAAACAG AAACCTTCAC AGAAACTTTT 
ATCCTTCGGG TTTATCTGCA GGAGCCAGAC TGTAGCATCA TCCGGATGAG CTCCAGGGTC 
CTGGAGGTGC CTGAGTTCTA TGGCCTCTCC AGAGCAATTG ATAAGAATGT GCTCAGTTTT 
GATTATGAGA GGAGGATGAG TCTGGAATGC ACCATAAGAC TCTCTCCATT AGAAACTGGC 
CTACCAGCCC ATGGTCAGCT GGTCATTGTG GAACCTCAGC AAGGAGAACC CCGAGGAGAC 
CAGCCCCACA GCTTTTTCCC TGAGTCTCAA TATAGGAAAA ATCCAGGATG CCCGAGTGGA 
AGCTGTTCCC CAGAGTTGAA GAAGATTGAT AGTCTCAAAG TCAGCTGTGA GGAGTTTCTG 
CTAATGGGGT TGCGATATCA GCACCTCCAT CCACCTTCTC CGAATGTCGA TTATATCTCC 
ATCAGACTGG ACCTCACTGA TATCAGGAGT AAATCCATAT ACAAGTCAGA GAGCATGTGG 
CTGCCTGTCT CCATCAAAGG TGCCTTCCCC AATCAGATCC CCAGGGCTGC ATTCATGCCC 
ACCTATATTC TAGAAGTGGA TCAATTCATC CTGAGCTCAG TGACCACTTC TACAGTGGAC 
TGCGAAGATG ATGAGACACC TAGATCTCTG TTGGTTTTCA ACATCACCAA GCCCCCTCTC 
CAGGGCTACT TCACACACCT ATTGGATCAC ACCAAAGAGA TCTCTTCATT CACCTGGAAA 
GATCTAAGTG ATTTGCAAAT TGCCTATCAG CCTCCCAACA GCAGCCACAC AGAGAGAAGG 
AATTATGAGG TGGAGTTTGA AGTGTACGAC TTCTTCTTTG AAAAGAGCTT ACCAATCACA 
GTCCATATTT CTATCAGGAC AGCAGACACG AATGCCCCAA GAGTGTCATG GAACACAGGT 
CTCAGTCTTC TAGAGGGACA GTCAAGGCCC ATCACCTGGG AGCAGTTTCA GATAGTGGAC 
AATGATGACA TCAAGGCAGT TCGCCTGGTC ACTGTTGATG GCTTACAGCA TGGGAGACTT 
ACTGTAAGAG GAGGGAAAGG CTTCATTTTC TCTGTAGCCG ACATTCAGGC TGGGACTGTT 
CGTTACCATC ATGATGACAG TGACTCCACC AAGGATTTTG TGGTCTTCAG GATTTTTGAT 
GGCCATCACA GCACTCGCCA CAAGTTTCCC ATCAATATCC TGCCTAAAGA TGACAGCCCG 
CCTCTCCTCA TAACAAATGT TGGGATTGAA TTAGACGAGG GGCAGACTGT GCTGATCCAA 
GGCTCCATGC TGAAAGCTTC TGACACGGAC TCCAGTGATG ACTACATTTT CTTTAATATC 
ACTAAACCTT CCCAGGCTGG AGAAATCATT AAGAAGCCAG GTCCAGGACT TATTGGTTAC 
CCAGTCCCTG GTTTTCTTCA GAGAGATCTG TTTAATGGGA TTATCTATTA TCGACACTTT 
GGTGGAGAAA CTTTTGAGGA TTCCTTTGAG CTTGTTCTTT GTGACAGCCA CGATCCTCCC 
AACCTCTCAG AACCACAGGT AGTGGTGATA CACATCACCC CAGTGGATGA TGAACTTCCA 
AAAGAGGTTC CTGGAGCGAT TCGGCATTTG GTTGTCAAGG AAACAGAGGT GGCTCATCTC 
ACTAAGAAGC ATCTACATTT TGTGGATACA GAATCTCAGG ATAGGGAGCT TCTCTACACA 
ATAACAAGAC CTCCATTTTC CTCCTTCAGA TATGACCATT CTGATGCTGG GAAATTGTTC 
ATGGTGGATA GCATATCGAA GCTAATCAAG GATCCTGCAG CTCTGGGACT GAGATCATTC 
ACCCAGCATG CTGTAAACTA CATGAAGGTG GCTTACATGC CACCCATGCA AGACATTGGA 
CCCTATGTTC AGCATGTTCA GTTTGTTTTT TCCATCAGTA ACCAGCATGG CGGCACTTTG 
CATGGAATTT GCTTTAATAT TACTATTCTA CCAGTGGATA ATGAGGCTCC TGAGGTGTTC 
ACCAACTCCC TGCAAGTAGA AGAAGGAGGT CTATGTATCA TCACAACAGA ACACATTCTG 
ATTTCAGATA TGGATACAAT ACAAGATAAA ATCAGACTGT CCCTCCAGAG AATGCCACTC 
CATGGAATGG TTGAACTGAA TGGTTTTCCT CTAAGCCAAG GAGACAAATT TTCATGGGGA 
GACCTCCATG CATTAAAAGT TAGGTATCAC CATGATGGAT CAGAGGTTTT TCATGACAAC 
ATACTCTTTG AGGCAACAGA TGGCACAAAT TCTGCAGAAT TTGTCCTTCA GGTCAAGGTT 
TTACCTGTAA ATGATGAACC CCCAGTCCTG AAGGCTGATC TCATTCCCAC ACTGCATTGT 
CCTGAGGGAG AGGAAGTGGT CATCTCTTCA GAACATGTTT ATGCCACTGA CATGGACAGC 
GATGACACCA AACTGATGTT CATGATTGTA CGTCAGCCTC ATCATGGGGT CGTGAGGAAA 
TCTGGGATCA CCGTGGACCA GTTCACCCAG GGAGACATTG ATGCTGGAAC TGTCACATAC 
AAACACACAA GTGGAGAGAT TGGCATGGCC CCTTGTTTTG ACACCATTTC TTTGGTGGTT 
TCGGATGGAG AAACTGGTCC TCAGGACTGT TGCTACCAAG ATCCCATTTC TTCACCACTT 
CCACCTCATC ATTCTTTTCC AGTGTATGAT CTCAATGTGA CTGTGTATCC TGTGGACAAC 
CAAAGACCAA CTATTACAAC TGGTACCCAA TTTGTTGTGG ATGAGGGTTC TTCAGCTGCC 
ATTAATGTTA ACCATTTAAT CACTACTGAC CCCGACACAG CCATGGATGA CTTACAGTTT 
GCTTTGGTTT CTCCTCCACA ATTTGGTTAT ATTGAAAACA TACTTCCTTC TCCAGGTTTT 
GAGAAAAGCA ATATGGGCAT AAGCATAGCT TCATTTTGGT GGAAAGACAT GAAGGCTTTG 
CATATAAACT ATGTGCAGTC CAGACATCTG AGGATAGAGC CAACAGCAGA TCACTTTATG 
ATTTACGTCA CAGATGGAAA ACAGAATTCT ATGGAAATCC CTTTTTCCAT CCTGATTAAC 
CCCACAAATG ATGAAGTTCC TGACTTTGTG ACACAGAACA TCACTGTTTC TGAGGGTCAG 
ATGAAAGAAC TGGATCTTTC AGTCATCAAA GCAACTGACC TAGACATGCC AAAAGATCAT 
TTGATGTTCA CCATTACCCA TAAACCACAG CATGGCCTCC TCATCAATGG GATACCTAGA 
AATGACATCC CTCATTATAA GCAATTAATC AATTCTCACC AGAACCATGA ACTTCCTGTG 
TGTGATTTTT CAATGGAGCT TCTTGAAACT GGAATGAAGT TGACATATAT ACACGATGAC 
TCTGAAAGCC TTGCTGATGA CTTTACAATC CAGTTGTCAG ATGGCAAACA TAAAGTCTTA 
AGAACAATTT CCATAAAGAT CCTGCCAGTT AATGATGAGA AACCAGTGCT GACCAAGAAG 
GCTGAAATAG AAATGAATAT GGGTGAAACT CGCATTATTT CTAGTGCTAT TCTATCAGCT 
ATGGATAAAG ACACACCCAG AGAGAAAATT TACTATTTAT TTGAAAGACT TCCAGAAAAT 
GGGCAGCTTC AGCTCAAGAT AGGGAGGAAT TGGGTATCTC TTCAACCTGG CATGAAATGT 
ACTCAAGAAG AAGTAGATCT GAATTTCATG AGATACATCC ATACTGGTGC AAAAGGCTCC 
CAGGACCGAG ATAGCTTCAC ATTTCATCTC TGGGATGGAG ACAACAGGTC ACCTACGCTG 
GACTGTCATA TCAGCATCAA GGACATCAAA AAGGGTGATA TTGTGGTCTT GACCAGACCA 
TTGGTAGTGT CTAAAGGGGG CAAAGGTTTC TTGACTACAT CTACTCTCTT AGCTACTGAT 
GGAACAGATA AGCCAGAAGA ACTTCTTTTT GTAATCACCT CCCCACCTCA ATATGGCCAG 
ATGGAATACA TCACTTATCC TGGAGTCCCC ATCACAAGCT TCAATCAGAT GGATATTGCA 
GGTCAGACCG TCTGTTATGT ACACAAGAGC AAGGCAACTG GTCCCAGTGA TATGTTCAGA 
TTCGTCTTGA GCAATGGACT AAAGACAAAG CATGGAGTGT TTAAGATCAA ACTGGAGTCT 
ATTGACCAAG CCTTGCCTGT TGTGACCCAG AACAAGGGGT TAAGAATAAT GGAAAGGACC 
ATGGCGCTCC TCTCTCCAGA CATCCTTCAA CTTTCTGACC CAGACACTCC AACAGAAAAC 
CTCACCTTCC TCTTGGCCCA GCTCCCCCAG TATGGACAGC TCTATCTTCG GGGGATGGTT 
CTCACACAAC ACAACTTTAC ACAACAAGAA GTGGACAGTA AGAATGTGGC CTACCAACAC 
CTAGGAGGAA ACTCACAGGT TGACCAGTTT ACCTTTGTAG CTACTGATGG GACAAATCAA 
GGCTTTGTTG TGGACGGAAG AGTGCAGAAT GAACCTGTGT CTTTCATTAT TCAGGTGTCC 
CAGTTGGATA AAAAAGCACC ACGCATCATC CAGCTTCAGT GCCCTTCCCA AGTGGAGCTT 
CTGAAAAATG GTTGCTATGG GATTTACATA ACATCTCGAG TCCTGAAGGC ATCTGACCCT 
GATACAGAGG ACAACCAAAT CATCTTTAAG ATTTTACGAG GCCCCCAGTA CGGACATCTG 
GAAAATACAA CAACAGGTGA ATTTATTCAG GAGAGATTCA GTCAAAAGGA CCTAAACAGT 
AAAATCATTC TTTATGTCAT CAATCCATCT TCAGAAATGA ATTCGGATAA TTTGGAGTTT 
CAAGTCACAG ACCCCAGTGG AAACACCGCT GCCCCTCAAA TTTTGGAACT AAAGTGGTCC 
CACATTCATT TGCCCCAGAC TGAATATGAA GTTTGTGAAA ACATGAACAC AGTACCACTG 
AAAATCACTA GGATGGGCCA TTTAATGGAA TCAGCCTTTG TTGGAGTAAA GGTTAATCAA 
GTATCTGCTA CAATTGGAAA AGATTTCACC GTGAGTCCAT CAAAGCTGAT TCAGTTTGAT 
CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT 
GATGAGGTCT TTGAAGTAAT TCTCAACTCC CCTGTGAATG CCGTTCTTGG CACGAAGACA 
AAAGCCAGAG TGAAAATTTT GGACTCAAAA GGAGGACAGT GCCTCCATTC ACATTCCTCT 
GGCCAAAACC AGTACAACTC ATGGGGGAAA GGCATATGGC TCCCACTTTC CCCAGGCTCA 
TCATCACCCT CCATTTCTGA TGGGATTCAT TTGGAAGGAA GACACCCTTC ATCTTCCAAA 
GAGGGGATAA CACACAGCAG AGGGGACAAG CTTCGGGGCT TTGACTTAAC TGATATTCCC 
AGGAAAAGGC TTAGGACAAT GGGAAATGGC AAAACAGTTC ACCCATCATC CATTTATAGA 
AATGAAACAG ATATAATTTT TAGGTATCAT GGTATGGTTG GATTAAGAGT AGAAGATGAC 
AAATCTGCCA TAAATAGAGA GAAGACCAGA GGGTCTATAA CTAATCAGAG GCTACAAAAG 
AAAAGCAGGA ACACAGAACC ACTATGGGCA GATAAGGTGG ACCAGATGCC CTATTTACAC 
TCCCCTATTC AGGACCAGTC ACCATCCTTT CCAAAAAATT GCACATTAGA ATTAAAGGGA 
CTCTTTCATT TTGAGGAAAG CTTCCAAAAA TTATATCATT GCAATGGAAC TTCTTGGAAG 
CCTTGGAGTC CAAAAGACCA GGATGGGAAT GAAAGAACCT GTCCCATTGG ATGGAGCCAT 
CAAAGCAGCC ATTGCTACTC ATTGATTACA GAGCAAAAGG TCACCTGGAA TATGGCTGCT 
CGGGCTTGCA GGGAACAACA TCAAGGCACC CTAGCAAGTG TGTTTACGAA ACAGCATATG 
CAGTGGCTAT GGGACTTCAG TGGAAGAAAA TCCTTCTGGA TAGGTTTGAA TGACCAAGAG 
AATGCAGATC GCTGGGAATG GGATGGAGGT GAACCTGTAA CCTTCACAAA CTGGAAGAGA 
GAGGCCCCCC CACATTTAAA GAAAAGAAAG AATTGCGTTT TGGTAAAAAG AAGAGGCAAG 
TGGCAAGCTA AAGACTGCAG GAAGGATAAA GCTCACAACT ACATGTGTGC CAGGAAACTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_001372782.3 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_001372819.1 Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

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CloneID	OMc28695
Clone ID Related Accession (Same CDS sequence)	XM_007499581.2 , XM_001372782.3
Accession Version	XM_001372782.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_001372782.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGC TTGGATCCAG TGGGGTGTTT CTCAGCCTGC TGCTTGTCCT GCAAAGTACC 
AACTCATCCT TCATCAGCGT CAACCAGGGC TTGAAGGTGG TGAAGGGCCA CTCTGCTTTC 
CTGTCAAGGG AGGACCTGCA GTTTTCCATT CCCAAAGAAA AAGATGCCTG CAAAGTGGAA 
GTTGTGCTAA ATGAACCAAT AACTCAGAGG GTTGGGAAAC TCACTCCACA GGTATTTGAC 
TGTCATTTCC TTCCTAATGA AGTAAAATAC ATTCATAATG GGTGCCCAAT CCTACATGAA 
GATGCTGTTA AACTTAGGCT CTACAGATTT ACAGAAACAG AAACCTTCAC AGAAACTTTT 
ATCCTTCGGG TTTATCTGCA GGAGCCAGAC TGTAGCATCA TCCGGATGAG CTCCAGGGTC 
CTGGAGGTGC CTGAGTTCTA TGGCCTCTCC AGAGCAATTG ATAAGAATGT GCTCAGTTTT 
GATTATGAGA GGAGGATGAG TCTGGAATGC ACCATAAGAC TCTCTCCATT AGAAACTGGC 
CTACCAGCCC ATGGTCAGCT GGTCATTGTG GAACCTCAGC AAGGAGAACC CCGAGGAGAC 
CAGCCCCACA GCTTTTTCCC TGAGTCTCAA TATAGGAAAA ATCCAGGATG CCCGAGTGGA 
AGCTGTTCCC CAGAGTTGAA GAAGATTGAT AGTCTCAAAG TCAGCTGTGA GGAGTTTCTG 
CTAATGGGGT TGCGATATCA GCACCTCCAT CCACCTTCTC CGAATGTCGA TTATATCTCC 
ATCAGACTGG ACCTCACTGA TATCAGGAGT AAATCCATAT ACAAGTCAGA GAGCATGTGG 
CTGCCTGTCT CCATCAAAGG TGCCTTCCCC AATCAGATCC CCAGGGCTGC ATTCATGCCC 
ACCTATATTC TAGAAGTGGA TCAATTCATC CTGAGCTCAG TGACCACTTC TACAGTGGAC 
TGCGAAGATG ATGAGACACC TAGATCTCTG TTGGTTTTCA ACATCACCAA GCCCCCTCTC 
CAGGGCTACT TCACACACCT ATTGGATCAC ACCAAAGAGA TCTCTTCATT CACCTGGAAA 
GATCTAAGTG ATTTGCAAAT TGCCTATCAG CCTCCCAACA GCAGCCACAC AGAGAGAAGG 
AATTATGAGG TGGAGTTTGA AGTGTACGAC TTCTTCTTTG AAAAGAGCTT ACCAATCACA 
GTCCATATTT CTATCAGGAC AGCAGACACG AATGCCCCAA GAGTGTCATG GAACACAGGT 
CTCAGTCTTC TAGAGGGACA GTCAAGGCCC ATCACCTGGG AGCAGTTTCA GATAGTGGAC 
AATGATGACA TCAAGGCAGT TCGCCTGGTC ACTGTTGATG GCTTACAGCA TGGGAGACTT 
ACTGTAAGAG GAGGGAAAGG CTTCATTTTC TCTGTAGCCG ACATTCAGGC TGGGACTGTT 
CGTTACCATC ATGATGACAG TGACTCCACC AAGGATTTTG TGGTCTTCAG GATTTTTGAT 
GGCCATCACA GCACTCGCCA CAAGTTTCCC ATCAATATCC TGCCTAAAGA TGACAGCCCG 
CCTCTCCTCA TAACAAATGT TGGGATTGAA TTAGACGAGG GGCAGACTGT GCTGATCCAA 
GGCTCCATGC TGAAAGCTTC TGACACGGAC TCCAGTGATG ACTACATTTT CTTTAATATC 
ACTAAACCTT CCCAGGCTGG AGAAATCATT AAGAAGCCAG GTCCAGGACT TATTGGTTAC 
CCAGTCCCTG GTTTTCTTCA GAGAGATCTG TTTAATGGGA TTATCTATTA TCGACACTTT 
GGTGGAGAAA CTTTTGAGGA TTCCTTTGAG CTTGTTCTTT GTGACAGCCA CGATCCTCCC 
AACCTCTCAG AACCACAGGT AGTGGTGATA CACATCACCC CAGTGGATGA TGAACTTCCA 
AAAGAGGTTC CTGGAGCGAT TCGGCATTTG GTTGTCAAGG AAACAGAGGT GGCTCATCTC 
ACTAAGAAGC ATCTACATTT TGTGGATACA GAATCTCAGG ATAGGGAGCT TCTCTACACA 
ATAACAAGAC CTCCATTTTC CTCCTTCAGA TATGACCATT CTGATGCTGG GAAATTGTTC 
ATGGTGGATA GCATATCGAA GCTAATCAAG GATCCTGCAG CTCTGGGACT GAGATCATTC 
ACCCAGCATG CTGTAAACTA CATGAAGGTG GCTTACATGC CACCCATGCA AGACATTGGA 
CCCTATGTTC AGCATGTTCA GTTTGTTTTT TCCATCAGTA ACCAGCATGG CGGCACTTTG 
CATGGAATTT GCTTTAATAT TACTATTCTA CCAGTGGATA ATGAGGCTCC TGAGGTGTTC 
ACCAACTCCC TGCAAGTAGA AGAAGGAGGT CTATGTATCA TCACAACAGA ACACATTCTG 
ATTTCAGATA TGGATACAAT ACAAGATAAA ATCAGACTGT CCCTCCAGAG AATGCCACTC 
CATGGAATGG TTGAACTGAA TGGTTTTCCT CTAAGCCAAG GAGACAAATT TTCATGGGGA 
GACCTCCATG CATTAAAAGT TAGGTATCAC CATGATGGAT CAGAGGTTTT TCATGACAAC 
ATACTCTTTG AGGCAACAGA TGGCACAAAT TCTGCAGAAT TTGTCCTTCA GGTCAAGGTT 
TTACCTGTAA ATGATGAACC CCCAGTCCTG AAGGCTGATC TCATTCCCAC ACTGCATTGT 
CCTGAGGGAG AGGAAGTGGT CATCTCTTCA GAACATGTTT ATGCCACTGA CATGGACAGC 
GATGACACCA AACTGATGTT CATGATTGTA CGTCAGCCTC ATCATGGGGT CGTGAGGAAA 
TCTGGGATCA CCGTGGACCA GTTCACCCAG GGAGACATTG ATGCTGGAAC TGTCACATAC 
AAACACACAA GTGGAGAGAT TGGCATGGCC CCTTGTTTTG ACACCATTTC TTTGGTGGTT 
TCGGATGGAG AAACTGGTCC TCAGGACTGT TGCTACCAAG ATCCCATTTC TTCACCACTT 
CCACCTCATC ATTCTTTTCC AGTGTATGAT CTCAATGTGA CTGTGTATCC TGTGGACAAC 
CAAAGACCAA CTATTACAAC TGGTACCCAA TTTGTTGTGG ATGAGGGTTC TTCAGCTGCC 
ATTAATGTTA ACCATTTAAT CACTACTGAC CCCGACACAG CCATGGATGA CTTACAGTTT 
GCTTTGGTTT CTCCTCCACA ATTTGGTTAT ATTGAAAACA TACTTCCTTC TCCAGGTTTT 
GAGAAAAGCA ATATGGGCAT AAGCATAGCT TCATTTTGGT GGAAAGACAT GAAGGCTTTG 
CATATAAACT ATGTGCAGTC CAGACATCTG AGGATAGAGC CAACAGCAGA TCACTTTATG 
ATTTACGTCA CAGATGGAAA ACAGAATTCT ATGGAAATCC CTTTTTCCAT CCTGATTAAC 
CCCACAAATG ATGAAGTTCC TGACTTTGTG ACACAGAACA TCACTGTTTC TGAGGGTCAG 
ATGAAAGAAC TGGATCTTTC AGTCATCAAA GCAACTGACC TAGACATGCC AAAAGATCAT 
TTGATGTTCA CCATTACCCA TAAACCACAG CATGGCCTCC TCATCAATGG GATACCTAGA 
AATGACATCC CTCATTATAA GCAATTAATC AATTCTCACC AGAACCATGA ACTTCCTGTG 
TGTGATTTTT CAATGGAGCT TCTTGAAACT GGAATGAAGT TGACATATAT ACACGATGAC 
TCTGAAAGCC TTGCTGATGA CTTTACAATC CAGTTGTCAG ATGGCAAACA TAAAGTCTTA 
AGAACAATTT CCATAAAGAT CCTGCCAGTT AATGATGAGA AACCAGTGCT GACCAAGAAG 
GCTGAAATAG AAATGAATAT GGGTGAAACT CGCATTATTT CTAGTGCTAT TCTATCAGCT 
ATGGATAAAG ACACACCCAG AGAGAAAATT TACTATTTAT TTGAAAGACT TCCAGAAAAT 
GGGCAGCTTC AGCTCAAGAT AGGGAGGAAT TGGGTATCTC TTCAACCTGG CATGAAATGT 
ACTCAAGAAG AAGTAGATCT GAATTTCATG AGATACATCC ATACTGGTGC AAAAGGCTCC 
CAGGACCGAG ATAGCTTCAC ATTTCATCTC TGGGATGGAG ACAACAGGTC ACCTACGCTG 
GACTGTCATA TCAGCATCAA GGACATCAAA AAGGGTGATA TTGTGGTCTT GACCAGACCA 
TTGGTAGTGT CTAAAGGGGG CAAAGGTTTC TTGACTACAT CTACTCTCTT AGCTACTGAT 
GGAACAGATA AGCCAGAAGA ACTTCTTTTT GTAATCACCT CCCCACCTCA ATATGGCCAG 
ATGGAATACA TCACTTATCC TGGAGTCCCC ATCACAAGCT TCAATCAGAT GGATATTGCA 
GGTCAGACCG TCTGTTATGT ACACAAGAGC AAGGCAACTG GTCCCAGTGA TATGTTCAGA 
TTCGTCTTGA GCAATGGACT AAAGACAAAG CATGGAGTGT TTAAGATCAA ACTGGAGTCT 
ATTGACCAAG CCTTGCCTGT TGTGACCCAG AACAAGGGGT TAAGAATAAT GGAAAGGACC 
ATGGCGCTCC TCTCTCCAGA CATCCTTCAA CTTTCTGACC CAGACACTCC AACAGAAAAC 
CTCACCTTCC TCTTGGCCCA GCTCCCCCAG TATGGACAGC TCTATCTTCG GGGGATGGTT 
CTCACACAAC ACAACTTTAC ACAACAAGAA GTGGACAGTA AGAATGTGGC CTACCAACAC 
CTAGGAGGAA ACTCACAGGT TGACCAGTTT ACCTTTGTAG CTACTGATGG GACAAATCAA 
GGCTTTGTTG TGGACGGAAG AGTGCAGAAT GAACCTGTGT CTTTCATTAT TCAGGTGTCC 
CAGTTGGATA AAAAAGCACC ACGCATCATC CAGCTTCAGT GCCCTTCCCA AGTGGAGCTT 
CTGAAAAATG GTTGCTATGG GATTTACATA ACATCTCGAG TCCTGAAGGC ATCTGACCCT 
GATACAGAGG ACAACCAAAT CATCTTTAAG ATTTTACGAG GCCCCCAGTA CGGACATCTG 
GAAAATACAA CAACAGGTGA ATTTATTCAG GAGAGATTCA GTCAAAAGGA CCTAAACAGT 
AAAATCATTC TTTATGTCAT CAATCCATCT TCAGAAATGA ATTCGGATAA TTTGGAGTTT 
CAAGTCACAG ACCCCAGTGG AAACACCGCT GCCCCTCAAA TTTTGGAACT AAAGTGGTCC 
CACATTCATT TGCCCCAGAC TGAATATGAA GTTTGTGAAA ACATGAACAC AGTACCACTG 
AAAATCACTA GGATGGGCCA TTTAATGGAA TCAGCCTTTG TTGGAGTAAA GGTTAATCAA 
GTATCTGCTA CAATTGGAAA AGATTTCACC GTGAGTCCAT CAAAGCTGAT TCAGTTTGAT 
CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT 
GATGAGGTCT TTGAAGTAAT TCTCAACTCC CCTGTGAATG CCGTTCTTGG CACGAAGACA 
AAAGCCAGAG TGAAAATTTT GGACTCAAAA GGAGGACAGT GCCTCCATTC ACATTCCTCT 
GGCCAAAACC AGTACAACTC ATGGGGGAAA GGCATATGGC TCCCACTTTC CCCAGGCTCA 
TCATCACCCT CCATTTCTGA TGGGATTCAT TTGGAAGGAA GACACCCTTC ATCTTCCAAA 
GAGGGGATAA CACACAGCAG AGGGGACAAG CTTCGGGGCT TTGACTTAAC TGATATTCCC 
AGGAAAAGGC TTAGGACAAT GGGAAATGGC AAAACAGTTC ACCCATCATC CATTTATAGA 
AATGAAACAG ATATAATTTT TAGGTATCAT GGTATGGTTG GATTAAGAGT AGAAGATGAC 
AAATCTGCCA TAAATAGAGA GAAGACCAGA GGGTCTATAA CTAATCAGAG GCTACAAAAG 
AAAAGCAGGA ACACAGAACC ACTATGGGCA GATAAGGTGG ACCAGATGCC CTATTTACAC 
TCCCCTATTC AGGACCAGTC ACCATCCTTT CCAAAAAATT GCACATTAGA ATTAAAGGGA 
CTCTTTCATT TTGAGGAAAG CTTCCAAAAA TTATATCATT GCAATGGAAC TTCTTGGAAG 
CCTTGGAGTC CAAAAGACCA GGATGGGAAT GAAAGAACCT GTCCCATTGG ATGGAGCCAT 
CAAAGCAGCC ATTGCTACTC ATTGATTACA GAGCAAAAGG TCACCTGGAA TATGGCTGCT 
CGGGCTTGCA GGGAACAACA TCAAGGCACC CTAGCAAGTG TGTTTACGAA ACAGCATATG 
CAGTGGCTAT GGGACTTCAG TGGAAGAAAA TCCTTCTGGA TAGGTTTGAA TGACCAAGAG 
AATGCAGATC GCTGGGAATG GGATGGAGGT GAACCTGTAA CCTTCACAAA CTGGAAGAGA 
GAGGCCCCCC CACATTTAAA GAAAAGAAAG AATTGCGTTT TGGTAAAAAG AAGAGGCAAG 
TGGCAAGCTA AAGACTGCAG GAAGGATAAA GCTCACAACT ACATGTGTGC CAGGAAACTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001372819.1
CDS	359..6901
Translation	MNLLGSSGVFLSLLLVLQSTNSSFISVNQGLKVVKGHSAFLSREDLQFSIPKEKDACKVEVVLNEPITQRVGKLTPQVFDCHFLPNEVKYIHNGCPILHEDAVKLRLYRFTETETFTETFILRVYLQEPDCSIIRMSSRVLEVPEFYGLSRAIDKNVLSFDYERRMSLECTIRLSPLETGLPAHGQLVIVEPQQGEPRGDQPHSFFPESQYRKNPGCPSGSCSPELKKIDSLKVSCEEFLLMGLRYQHLHPPSPNVDYISIRLDLTDIRSKSIYKSESMWLPVSIKGAFPNQIPRAAFMPTYILEVDQFILSSVTTSTVDCEDDETPRSLLVFNITKPPLQGYFTHLLDHTKEISSFTWKDLSDLQIAYQPPNSSHTERRNYEVEFEVYDFFFEKSLPITVHISIRTADTNAPRVSWNTGLSLLEGQSRPITWEQFQIVDNDDIKAVRLVTVDGLQHGRLTVRGGKGFIFSVADIQAGTVRYHHDDSDSTKDFVVFRIFDGHHSTRHKFPINILPKDDSPPLLITNVGIELDEGQTVLIQGSMLKASDTDSSDDYIFFNITKPSQAGEIIKKPGPGLIGYPVPGFLQRDLFNGIIYYRHFGGETFEDSFELVLCDSHDPPNLSEPQVVVIHITPVDDELPKEVPGAIRHLVVKETEVAHLTKKHLHFVDTESQDRELLYTITRPPFSSFRYDHSDAGKLFMVDSISKLIKDPAALGLRSFTQHAVNYMKVAYMPPMQDIGPYVQHVQFVFSISNQHGGTLHGICFNITILPVDNEAPEVFTNSLQVEEGGLCIITTEHILISDMDTIQDKIRLSLQRMPLHGMVELNGFPLSQGDKFSWGDLHALKVRYHHDGSEVFHDNILFEATDGTNSAEFVLQVKVLPVNDEPPVLKADLIPTLHCPEGEEVVISSEHVYATDMDSDDTKLMFMIVRQPHHGVVRKSGITVDQFTQGDIDAGTVTYKHTSGEIGMAPCFDTISLVVSDGETGPQDCCYQDPISSPLPPHHSFPVYDLNVTVYPVDNQRPTITTGTQFVVDEGSSAAINVNHLITTDPDTAMDDLQFALVSPPQFGYIENILPSPGFEKSNMGISIASFWWKDMKALHINYVQSRHLRIEPTADHFMIYVTDGKQNSMEIPFSILINPTNDEVPDFVTQNITVSEGQMKELDLSVIKATDLDMPKDHLMFTITHKPQHGLLINGIPRNDIPHYKQLINSHQNHELPVCDFSMELLETGMKLTYIHDDSESLADDFTIQLSDGKHKVLRTISIKILPVNDEKPVLTKKAEIEMNMGETRIISSAILSAMDKDTPREKIYYLFERLPENGQLQLKIGRNWVSLQPGMKCTQEEVDLNFMRYIHTGAKGSQDRDSFTFHLWDGDNRSPTLDCHISIKDIKKGDIVVLTRPLVVSKGGKGFLTTSTLLATDGTDKPEELLFVITSPPQYGQMEYITYPGVPITSFNQMDIAGQTVCYVHKSKATGPSDMFRFVLSNGLKTKHGVFKIKLESIDQALPVVTQNKGLRIMERTMALLSPDILQLSDPDTPTENLTFLLAQLPQYGQLYLRGMVLTQHNFTQQEVDSKNVAYQHLGGNSQVDQFTFVATDGTNQGFVVDGRVQNEPVSFIIQVSQLDKKAPRIIQLQCPSQVELLKNGCYGIYITSRVLKASDPDTEDNQIIFKILRGPQYGHLENTTTGEFIQERFSQKDLNSKIILYVINPSSEMNSDNLEFQVTDPSGNTAAPQILELKWSHIHLPQTEYEVCENMNTVPLKITRMGHLMESAFVGVKVNQVSATIGKDFTVSPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKARVKILDSKGGQCLHSHSSGQNQYNSWGKGIWLPLSPGSSSPSISDGIHLEGRHPSSSKEGITHSRGDKLRGFDLTDIPRKRLRTMGNGKTVHPSSIYRNETDIIFRYHGMVGLRVEDDKSAINREKTRGSITNQRLQKKSRNTEPLWADKVDQMPYLHSPIQDQSPSFPKNCTLELKGLFHFEESFQKLYHCNGTSWKPWSPKDQDGNERTCPIGWSHQSSHCYSLITEQKVTWNMAARACREQHQGTLASVFTKQHMQWLWDFSGRKSFWIGLNDQENADRWEWDGGEPVTFTNWKREAPPHLKKRKNCVLVKRRGKWQAKDCRKDKAHNYMCARKL

Target ORF information:

RefSeq Version	XM_001372782.3
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001372782.3

ORF Insert Sequence:

ATGAACCTGC TTGGATCCAG TGGGGTGTTT CTCAGCCTGC TGCTTGTCCT GCAAAGTACC 
AACTCATCCT TCATCAGCGT CAACCAGGGC TTGAAGGTGG TGAAGGGCCA CTCTGCTTTC 
CTGTCAAGGG AGGACCTGCA GTTTTCCATT CCCAAAGAAA AAGATGCCTG CAAAGTGGAA 
GTTGTGCTAA ATGAACCAAT AACTCAGAGG GTTGGGAAAC TCACTCCACA GGTATTTGAC 
TGTCATTTCC TTCCTAATGA AGTAAAATAC ATTCATAATG GGTGCCCAAT CCTACATGAA 
GATGCTGTTA AACTTAGGCT CTACAGATTT ACAGAAACAG AAACCTTCAC AGAAACTTTT 
ATCCTTCGGG TTTATCTGCA GGAGCCAGAC TGTAGCATCA TCCGGATGAG CTCCAGGGTC 
CTGGAGGTGC CTGAGTTCTA TGGCCTCTCC AGAGCAATTG ATAAGAATGT GCTCAGTTTT 
GATTATGAGA GGAGGATGAG TCTGGAATGC ACCATAAGAC TCTCTCCATT AGAAACTGGC 
CTACCAGCCC ATGGTCAGCT GGTCATTGTG GAACCTCAGC AAGGAGAACC CCGAGGAGAC 
CAGCCCCACA GCTTTTTCCC TGAGTCTCAA TATAGGAAAA ATCCAGGATG CCCGAGTGGA 
AGCTGTTCCC CAGAGTTGAA GAAGATTGAT AGTCTCAAAG TCAGCTGTGA GGAGTTTCTG 
CTAATGGGGT TGCGATATCA GCACCTCCAT CCACCTTCTC CGAATGTCGA TTATATCTCC 
ATCAGACTGG ACCTCACTGA TATCAGGAGT AAATCCATAT ACAAGTCAGA GAGCATGTGG 
CTGCCTGTCT CCATCAAAGG TGCCTTCCCC AATCAGATCC CCAGGGCTGC ATTCATGCCC 
ACCTATATTC TAGAAGTGGA TCAATTCATC CTGAGCTCAG TGACCACTTC TACAGTGGAC 
TGCGAAGATG ATGAGACACC TAGATCTCTG TTGGTTTTCA ACATCACCAA GCCCCCTCTC 
CAGGGCTACT TCACACACCT ATTGGATCAC ACCAAAGAGA TCTCTTCATT CACCTGGAAA 
GATCTAAGTG ATTTGCAAAT TGCCTATCAG CCTCCCAACA GCAGCCACAC AGAGAGAAGG 
AATTATGAGG TGGAGTTTGA AGTGTACGAC TTCTTCTTTG AAAAGAGCTT ACCAATCACA 
GTCCATATTT CTATCAGGAC AGCAGACACG AATGCCCCAA GAGTGTCATG GAACACAGGT 
CTCAGTCTTC TAGAGGGACA GTCAAGGCCC ATCACCTGGG AGCAGTTTCA GATAGTGGAC 
AATGATGACA TCAAGGCAGT TCGCCTGGTC ACTGTTGATG GCTTACAGCA TGGGAGACTT 
ACTGTAAGAG GAGGGAAAGG CTTCATTTTC TCTGTAGCCG ACATTCAGGC TGGGACTGTT 
CGTTACCATC ATGATGACAG TGACTCCACC AAGGATTTTG TGGTCTTCAG GATTTTTGAT 
GGCCATCACA GCACTCGCCA CAAGTTTCCC ATCAATATCC TGCCTAAAGA TGACAGCCCG 
CCTCTCCTCA TAACAAATGT TGGGATTGAA TTAGACGAGG GGCAGACTGT GCTGATCCAA 
GGCTCCATGC TGAAAGCTTC TGACACGGAC TCCAGTGATG ACTACATTTT CTTTAATATC 
ACTAAACCTT CCCAGGCTGG AGAAATCATT AAGAAGCCAG GTCCAGGACT TATTGGTTAC 
CCAGTCCCTG GTTTTCTTCA GAGAGATCTG TTTAATGGGA TTATCTATTA TCGACACTTT 
GGTGGAGAAA CTTTTGAGGA TTCCTTTGAG CTTGTTCTTT GTGACAGCCA CGATCCTCCC 
AACCTCTCAG AACCACAGGT AGTGGTGATA CACATCACCC CAGTGGATGA TGAACTTCCA 
AAAGAGGTTC CTGGAGCGAT TCGGCATTTG GTTGTCAAGG AAACAGAGGT GGCTCATCTC 
ACTAAGAAGC ATCTACATTT TGTGGATACA GAATCTCAGG ATAGGGAGCT TCTCTACACA 
ATAACAAGAC CTCCATTTTC CTCCTTCAGA TATGACCATT CTGATGCTGG GAAATTGTTC 
ATGGTGGATA GCATATCGAA GCTAATCAAG GATCCTGCAG CTCTGGGACT GAGATCATTC 
ACCCAGCATG CTGTAAACTA CATGAAGGTG GCTTACATGC CACCCATGCA AGACATTGGA 
CCCTATGTTC AGCATGTTCA GTTTGTTTTT TCCATCAGTA ACCAGCATGG CGGCACTTTG 
CATGGAATTT GCTTTAATAT TACTATTCTA CCAGTGGATA ATGAGGCTCC TGAGGTGTTC 
ACCAACTCCC TGCAAGTAGA AGAAGGAGGT CTATGTATCA TCACAACAGA ACACATTCTG 
ATTTCAGATA TGGATACAAT ACAAGATAAA ATCAGACTGT CCCTCCAGAG AATGCCACTC 
CATGGAATGG TTGAACTGAA TGGTTTTCCT CTAAGCCAAG GAGACAAATT TTCATGGGGA 
GACCTCCATG CATTAAAAGT TAGGTATCAC CATGATGGAT CAGAGGTTTT TCATGACAAC 
ATACTCTTTG AGGCAACAGA TGGCACAAAT TCTGCAGAAT TTGTCCTTCA GGTCAAGGTT 
TTACCTGTAA ATGATGAACC CCCAGTCCTG AAGGCTGATC TCATTCCCAC ACTGCATTGT 
CCTGAGGGAG AGGAAGTGGT CATCTCTTCA GAACATGTTT ATGCCACTGA CATGGACAGC 
GATGACACCA AACTGATGTT CATGATTGTA CGTCAGCCTC ATCATGGGGT CGTGAGGAAA 
TCTGGGATCA CCGTGGACCA GTTCACCCAG GGAGACATTG ATGCTGGAAC TGTCACATAC 
AAACACACAA GTGGAGAGAT TGGCATGGCC CCTTGTTTTG ACACCATTTC TTTGGTGGTT 
TCGGATGGAG AAACTGGTCC TCAGGACTGT TGCTACCAAG ATCCCATTTC TTCACCACTT 
CCACCTCATC ATTCTTTTCC AGTGTATGAT CTCAATGTGA CTGTGTATCC TGTGGACAAC 
CAAAGACCAA CTATTACAAC TGGTACCCAA TTTGTTGTGG ATGAGGGTTC TTCAGCTGCC 
ATTAATGTTA ACCATTTAAT CACTACTGAC CCCGACACAG CCATGGATGA CTTACAGTTT 
GCTTTGGTTT CTCCTCCACA ATTTGGTTAT ATTGAAAACA TACTTCCTTC TCCAGGTTTT 
GAGAAAAGCA ATATGGGCAT AAGCATAGCT TCATTTTGGT GGAAAGACAT GAAGGCTTTG 
CATATAAACT ATGTGCAGTC CAGACATCTG AGGATAGAGC CAACAGCAGA TCACTTTATG 
ATTTACGTCA CAGATGGAAA ACAGAATTCT ATGGAAATCC CTTTTTCCAT CCTGATTAAC 
CCCACAAATG ATGAAGTTCC TGACTTTGTG ACACAGAACA TCACTGTTTC TGAGGGTCAG 
ATGAAAGAAC TGGATCTTTC AGTCATCAAA GCAACTGACC TAGACATGCC AAAAGATCAT 
TTGATGTTCA CCATTACCCA TAAACCACAG CATGGCCTCC TCATCAATGG GATACCTAGA 
AATGACATCC CTCATTATAA GCAATTAATC AATTCTCACC AGAACCATGA ACTTCCTGTG 
TGTGATTTTT CAATGGAGCT TCTTGAAACT GGAATGAAGT TGACATATAT ACACGATGAC 
TCTGAAAGCC TTGCTGATGA CTTTACAATC CAGTTGTCAG ATGGCAAACA TAAAGTCTTA 
AGAACAATTT CCATAAAGAT CCTGCCAGTT AATGATGAGA AACCAGTGCT GACCAAGAAG 
GCTGAAATAG AAATGAATAT GGGTGAAACT CGCATTATTT CTAGTGCTAT TCTATCAGCT 
ATGGATAAAG ACACACCCAG AGAGAAAATT TACTATTTAT TTGAAAGACT TCCAGAAAAT 
GGGCAGCTTC AGCTCAAGAT AGGGAGGAAT TGGGTATCTC TTCAACCTGG CATGAAATGT 
ACTCAAGAAG AAGTAGATCT GAATTTCATG AGATACATCC ATACTGGTGC AAAAGGCTCC 
CAGGACCGAG ATAGCTTCAC ATTTCATCTC TGGGATGGAG ACAACAGGTC ACCTACGCTG 
GACTGTCATA TCAGCATCAA GGACATCAAA AAGGGTGATA TTGTGGTCTT GACCAGACCA 
TTGGTAGTGT CTAAAGGGGG CAAAGGTTTC TTGACTACAT CTACTCTCTT AGCTACTGAT 
GGAACAGATA AGCCAGAAGA ACTTCTTTTT GTAATCACCT CCCCACCTCA ATATGGCCAG 
ATGGAATACA TCACTTATCC TGGAGTCCCC ATCACAAGCT TCAATCAGAT GGATATTGCA 
GGTCAGACCG TCTGTTATGT ACACAAGAGC AAGGCAACTG GTCCCAGTGA TATGTTCAGA 
TTCGTCTTGA GCAATGGACT AAAGACAAAG CATGGAGTGT TTAAGATCAA ACTGGAGTCT 
ATTGACCAAG CCTTGCCTGT TGTGACCCAG AACAAGGGGT TAAGAATAAT GGAAAGGACC 
ATGGCGCTCC TCTCTCCAGA CATCCTTCAA CTTTCTGACC CAGACACTCC AACAGAAAAC 
CTCACCTTCC TCTTGGCCCA GCTCCCCCAG TATGGACAGC TCTATCTTCG GGGGATGGTT 
CTCACACAAC ACAACTTTAC ACAACAAGAA GTGGACAGTA AGAATGTGGC CTACCAACAC 
CTAGGAGGAA ACTCACAGGT TGACCAGTTT ACCTTTGTAG CTACTGATGG GACAAATCAA 
GGCTTTGTTG TGGACGGAAG AGTGCAGAAT GAACCTGTGT CTTTCATTAT TCAGGTGTCC 
CAGTTGGATA AAAAAGCACC ACGCATCATC CAGCTTCAGT GCCCTTCCCA AGTGGAGCTT 
CTGAAAAATG GTTGCTATGG GATTTACATA ACATCTCGAG TCCTGAAGGC ATCTGACCCT 
GATACAGAGG ACAACCAAAT CATCTTTAAG ATTTTACGAG GCCCCCAGTA CGGACATCTG 
GAAAATACAA CAACAGGTGA ATTTATTCAG GAGAGATTCA GTCAAAAGGA CCTAAACAGT 
AAAATCATTC TTTATGTCAT CAATCCATCT TCAGAAATGA ATTCGGATAA TTTGGAGTTT 
CAAGTCACAG ACCCCAGTGG AAACACCGCT GCCCCTCAAA TTTTGGAACT AAAGTGGTCC 
CACATTCATT TGCCCCAGAC TGAATATGAA GTTTGTGAAA ACATGAACAC AGTACCACTG 
AAAATCACTA GGATGGGCCA TTTAATGGAA TCAGCCTTTG TTGGAGTAAA GGTTAATCAA 
GTATCTGCTA CAATTGGAAA AGATTTCACC GTGAGTCCAT CAAAGCTGAT TCAGTTTGAT 
CCAGGAATGT CAACTAAGAT GTGGAATATA GCAATTACCT ATGACGGATT AGAGGAAGAT 
GATGAGGTCT TTGAAGTAAT TCTCAACTCC CCTGTGAATG CCGTTCTTGG CACGAAGACA 
AAAGCCAGAG TGAAAATTTT GGACTCAAAA GGAGGACAGT GCCTCCATTC ACATTCCTCT 
GGCCAAAACC AGTACAACTC ATGGGGGAAA GGCATATGGC TCCCACTTTC CCCAGGCTCA 
TCATCACCCT CCATTTCTGA TGGGATTCAT TTGGAAGGAA GACACCCTTC ATCTTCCAAA 
GAGGGGATAA CACACAGCAG AGGGGACAAG CTTCGGGGCT TTGACTTAAC TGATATTCCC 
AGGAAAAGGC TTAGGACAAT GGGAAATGGC AAAACAGTTC ACCCATCATC CATTTATAGA 
AATGAAACAG ATATAATTTT TAGGTATCAT GGTATGGTTG GATTAAGAGT AGAAGATGAC 
AAATCTGCCA TAAATAGAGA GAAGACCAGA GGGTCTATAA CTAATCAGAG GCTACAAAAG 
AAAAGCAGGA ACACAGAACC ACTATGGGCA GATAAGGTGG ACCAGATGCC CTATTTACAC 
TCCCCTATTC AGGACCAGTC ACCATCCTTT CCAAAAAATT GCACATTAGA ATTAAAGGGA 
CTCTTTCATT TTGAGGAAAG CTTCCAAAAA TTATATCATT GCAATGGAAC TTCTTGGAAG 
CCTTGGAGTC CAAAAGACCA GGATGGGAAT GAAAGAACCT GTCCCATTGG ATGGAGCCAT 
CAAAGCAGCC ATTGCTACTC ATTGATTACA GAGCAAAAGG TCACCTGGAA TATGGCTGCT 
CGGGCTTGCA GGGAACAACA TCAAGGCACC CTAGCAAGTG TGTTTACGAA ACAGCATATG 
CAGTGGCTAT GGGACTTCAG TGGAAGAAAA TCCTTCTGGA TAGGTTTGAA TGACCAAGAG 
AATGCAGATC GCTGGGAATG GGATGGAGGT GAACCTGTAA CCTTCACAAA CTGGAAGAGA 
GAGGCCCCCC CACATTTAAA GAAAAGAAAG AATTGCGTTT TGGTAAAAAG AAGAGGCAAG 
TGGCAAGCTA AAGACTGCAG GAAGGATAAA GCTCACAACT ACATGTGTGC CAGGAAACTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_016424261.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016279747.1 Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

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CloneID	OMc44199
Clone ID Related Accession (Same CDS sequence)	XM_016424261.1
Accession Version	XM_016424261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008806.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGC TTGGATCCAG TGGGGTGTTT CTCAGCCTGC TGCTTGTCCT GCAAAGTACC 
AACTCATCCT TCATCAGCGT CAACCAGGGC TTGAAGGTGG TGAAGGGCCA CTCTGCTTTC 
CTGTCAAGGG AGGACCTGCA GTTTTCCATT CCCAAAGAAA AAGATGCCTG CAAAGTGGAA 
GTTGTGCTAA ATGAACCAAT AACTCAGAGG GTTGGGAAAC TCACTCCACA GGTATTTGAC 
TGTCATTTCC TTCCTAATGA AGTAAAATAC ATTCATAATG GGTGCCCAAT CCTACATGAA 
GATGCTGTTA AACTTAGGCT CTACAGATTT ACAGAAACAG AAACCTTCAC AGAAACTTTT 
ATCCTTCGGG TTTATCTGCA GGAGCCAGAC TGTAGCATCA TCCGGATGAG CTCCAGGGTC 
CTGGAGGTGC CTGAGTTCTA TGGCCTCTCC AGAGCAATTG ATAAGAATGT GCTCAGTTTT 
GATTATGAGA GGAGGATGAG TCTGGAATGC ACCATAAGAC TCTCTCCATT AGAAACTGGC 
CTACCAGCCC ATGGTCAGCT GGTCATTGTG GAACCTCAGC AAGGAGAACC CCGAGGAGAC 
CAGCCCCACA GCTTTTTCCC TGAGTCTCAA TATAGGAAAA ATCCAGGATG CCCGAGTGGA 
AGCTGTTCCC CAGAGTTGAA GAAGATTGAT AGTCTCAAAG TCAGCTGTGA GGAGTTTCTG 
CTAATGGGGT TGCGATATCA GCACCTCCAT CCACCTTCTC CGAATGTCGA TTATATCTCC 
ATCAGACTGG ACCTCACTGA TATCAGGAGT AAATCCATAT ACAAGTCAGA GAGCATGTGG 
CTGCCTGTCT CCATCAAAGG TGCCTTCCCC AATCAGATCC CCAGGGCTGC ATTCATGCCC 
ACCTATATTC TAGAAGTGGA TCAATTCATC CTGAGCTCAG TGACCACTTC TACAGTGGAC 
TGCGAAGATG ATGAGACACC TAGATCTCTG TTGGTTTTCA ACATCACCAA GCCCCCTCTC 
CAGGGCTACT TCACACACCT ATTGGATCAC ACCAAAGAGA TCTCTTCATT CACCTGGAAA 
GATCTAAGTG ATTTGCAAAT TGCCTATCAG CCTCCCAACA GCAGCCACAC AGAGAGAAGG 
AATTATGAGG TGGAGTTTGA AGTGTACGAC TTCTTCTTTG AAAAGAGCTT ACCAATCACA 
GTCCATATTT CTATCAGGAC AGCAGACACG AATGCCCCAA GAGTGTCATG GAACACAGGT 
CTCAGTCTTC TAGAGGGACA GTCAAGGCCC ATCACCTGGG AGCAGTTTCA GATAGTGGAC 
AATGATGACA TCAAGGCAGT TCGCCTGGTC ACTGTTGATG GCTTACAGCA TGGGAGACTT 
ACTGTAAGAG GAGGGAAAGG CTTCATTTTC TCTGTAGCCG ACATTCAGGC TGGGACTGTT 
CGTTACCATC ATGATGACAG TGACTCCACC AAGGATTTTG TGGTCTTCAG GATTTTTGAT 
GGCCATCACA GCACTCGCCA CAAGTTTCCC ATCAATATCC TGCCTAAAGA TGACAGCCCG 
CCTCTCCTCA TAACAAATGT TGGGATTGAA TTAGACGAGG GGCAGACTGT GCTGATCCAA 
GGCTCCATGC TGAAAGCTTC TGACACGGAC TCCAGTGATG ACTACATTTT CTTTAATATC 
ACTAAACCTT CCCAGGCTGG AGAAATCATT AAGAAGCCAG GTCCAGGACT TATTGGTTAC 
CCAGTCCCTG GTTTTCTTCA GAGAGATCTG TTTAATGGGA TTATCTATTA TCGACACTTT 
GGTGGAGAAA CTTTTGAGGA TTCCTTTGAG CTTGTTCTTT GTGACAGCCA CGATCCTCCC 
AACCTCTCAG AACCACAGGT AGTGGTGATA CACATCACCC CAGTGGATGA TGAACTTCCA 
AAAGAGGTTC CTGGAGCGAT TCGGCATTTG GTTGTCAAGG AAACAGAGGT GGCTCATCTC 
ACTAAGAAGC ATCTACATTT TGTGGATACA GAATCTCAGG ATAGGGAGCT TCTCTACACA 
ATAACAAGAC CTCCATTTTC CTCCTTCAGA TATGACCATT CTGATGCTGG GAAATTGTTC 
ATGGTGGATA GCATATCGAA GCTAATCAAG GATCCTGCAG CTCTGGGACT GAGATCATTC 
ACCCAGCATG CTGTAAACTA CATGAAGGTG GCTTACATGC CACCCATGCA AGACATTGGA 
CCCTATGTTC AGCATGTTCA GTTTGTTTTT TCCATCAGTA ACCAGCATGG CGGCACTTTG 
CATGGAATTT GCTTTAATAT TACTATTCTA CCAGTGGATA ATGAGGCTCC TGAGGTGTTC 
ACCAACTCCC TGCAAGTAGA AGAAGGAGGT CTATGTATCA TCACAACAGA ACACATTCTG 
ATTTCAGATA TGGATACAAT ACAAGATAAA ATCAGACTGT CCCTCCAGAG AATGCCACTC 
CATGGAATGG TTGAACTGAA TGGTTTTCCT CTAAGCCAAG GAGACAAATT TTCATGGGGA 
GACCTCCATG CATTAAAAGT TAGGTATCAC CATGATGGAT CAGAGGTTTT TCATGACAAC 
ATACTCTTTG AGGCAACAGA TGGCACAAAT TCTGCAGAAT TTGTCCTTCA GGTCAAGGTT 
TTACCTGTAA ATGATGAACC CCCAGTCCTG AAGGCTGATC TCATTCCCAC ACTGCATTGT 
CCTGAGGGAG AGGAAGTGGT CATCTCTTCA GAACATGTTT ATGCCACTGA CATGGACAGC 
GATGACACCA AACTGATGTT CATGATTGTA CGTCAGCCTC ATCATGGGGT CGTGAGGAAA 
TCTGGGATCA CCGTGGACCA GTTCACCCAG GGAGACATTG ATGCTGGAAC TGTCACATAC 
AAACACACAA GTGGAGAGAT TGGCATGGCC CCTTGTTTTG ACACCATTTC TTTGGTGGTT 
TCGGATGGAG AAACTGGTCC TCAGGACTGT TGCTACCAAG ATCCCATTTC TTCACCACTT 
CCACCTCATC ATTCTTTTCC AGTGTATGAT CTCAATGTGA CTGTGTATCC TGTGGACAAC 
CAAAGACCAA CTATTACAAC TGGTACCCAA TTTGTTGTGG ATGAGGGTTC TTCAGCTGCC 
ATTAATGTTA ACCATTTAAT CACTACTGAC CCCGACACAG CCATGGATGA CTTACAGTTT 
GCTTTGGTTT CTCCTCCACA ATTTGGTTAT ATTGAAAACA TACTTCCTTC TCCAGGTTTT 
GAGAAAAGCA ATATGGGCAT AAGCATAGCT TCATTTTGGT GGAAAGACAT GAAGGCTTTG 
CATATAAACT ATGTGCAGTC CAGACATCTG AGGATAGAGC CAACAGCAGA TCACTTTATG 
ATTTACGTCA CAGATGGAAA ACAGAATTCT ATGGAAATCC CTTTTTCCAT CCTGATTAAC 
CCCACAAATG ATGAAGTTCC TGACTTTGTG ACACAGAACA TCACTGTTTC TGAGGGTCAG 
ATGAAAGAAC TGGATCTTTC AGTCATCAAA GCAACTGACC TAGACATGCC AAAAGATCAT 
TTGATGTTCA CCATTACCCA TAAACCACAG CATGGCCTCC TCATCAATGG GATACCTAGA 
AATGACATCC CTCATTATAA GCAATTAATC AATTCTCACC AGAACCATGA ACTTCCTGTG 
TGTGATTTTT CAATGGAGCT TCTTGAAACT GGAATGAAGT TGACATATAT ACACGATGAC 
TCTGAAAGCC TTGCTGATGA CTTTACAATC CAGTTGTCAG ATGGCAAACA TAAAGTCTTA 
AGAACAATTT CCATAAAGAT CCTGCCAGTT AATGATGAGA AACCAGTGCT GACCAAGAAG 
GCTGAAATAG AAATGAATAT GGGTGAAACT CGCATTATTT CTAGTGCTAT TCTATCAGCT 
ATGGATAAAG ACACACCCAG AGAGAAAATT TACTATTTAT TTGAAAGACT TCCAGAAAAT 
GGGCAGCTTC AGCTCAAGAT GGTGAAACAA GCATCCATGC TGAAGGCTAC CTTGCCTCTC 
TTTGCCAGAT TCGTCTTGAG CAATGGACTA AAGACAAAGC ATGGAGTGTT TAAGATCAAA 
CTGGAGTCTA TTGACCAAGC CTTGCCTGTT GTGACCCAGA ACAAGGGGTT AAGAATAATG 
GAAAGGACCA TGGCGCTCCT CTCTCCAGAC ATCCTTCAAC TTTCTGACCC AGACACTCCA 
ACAGAAAACC TCACCTTCCT CTTGGCCCAG CTCCCCCAGT ATGGACAGCT CTATCTTCGG 
GGGATGGTTC TCACACAACA CAACTTTACA CAACAAGAAG TGGACAGTAA GAATGTGGCC 
TACCAACACC TAGGAGGAAA CTCACAGGTT GACCAGTTTA CCTTTGTAGC TACTGATGGG 
ACAAATCAAG GCTTTGTTGT GGACGGAAGA GTGCAGAATG AACCTGTGTC TTTCATTATT 
CAGGTGTCCC AGTTGGATAA AAAAGCACCA CGCATCATCC AGCTTCAGTG CCCTTCCCAA 
GTGGAGCTTC TGAAAAATGG TTGCTATGGG ATTTACATAA CATCTCGAGT CCTGAAGGCA 
TCTGACCCTG ATACAGAGGA CAACCAAATC ATCTTTAAGA TTTTACGAGG CCCCCAGTAC 
GGACATCTGG AAAATACAAC AACAGGTGAA TTTATTCAGG AGAGATTCAG TCAAAAGGAC 
CTAAACAGTA AAATCATTCT TTATGTCATC AATCCATCTT CAGAAATGAA TTCGGATAAT 
TTGGAGTTTC AAGTCACAGA CCCCAGTGGA AACACCGCTG CCCCTCAAAT TTTGGAACTA 
AAGTGGTCCC ACATTCATTT GCCCCAGACT GAATATGAAG TTTGTGAAAA CATGAACACA 
GTACCACTGA AAATCACTAG GATGGGCCAT TTAATGGAAT CAGCCTTTGT TGGAGTAAAG 
GTTAATCAAG TATCTGCTAC AATTGGAAAA GATTTCACCG TGAGTCCATC AAAGCTGATT 
CAGTTTGATC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA 
GAGGAAGATG ATGAGGTCTT TGAAGTAATT CTCAACTCCC CTGTGAATGC CGTTCTTGGC 
ACGAAGACAA AAGCCAGAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG CCTCCATTCA 
CATTCCTCTG GCCAAAACCA GTACAACTCA TGGGGGAAAG GCATATGGCT CCCACTTTCC 
CCAGGCTCAT CATCACCCTC CATTTCTGAT GGGATTCATT TGGAAGGAAG ACACCCTTCA 
TCTTCCAAAG AGGGGATAAC ACACAGCAGA GGGGACAAGC TTCGGGGCTT TGACTTAACT 
GATATTCCCA GGAAAAGGCT TAGGACAATG GGAAATGGCA AAACAGTTCA CCCATCATCC 
ATTTATAGAA ATGAAACAGA TATAATTTTT AGGTATCATG GTATGGTTGG ATTAAGAGTA 
GAAGATGACA AATCTGCCAT AAATAGAGAG AAGACCAGAG GGTCTATAAC TAATCAGAGG 
CTACAAAAGA AAAGCAGGAA CACAGAACCA CTATGGGCAG ATAAGGTGGA CCAGATGCCC 
TATTTACACT CCCCTATTCA GGACCAGTCA CCATCCTTTC CAAAAAATTG CACATTAGAA 
TTAAAGGGAC TCTTTCATTT TGAGGAAAGC TTCCAAAAAT TATATCATTG CAATGGAACT 
TCTTGGAAGC CTTGGAGTCC AAAAGACCAG GATGGGAATG AAAGAACCTG TCCCATTGGA 
TGGAGCCATC AAAGCAGCCA TTGCTACTCA TTGATTACAG AGCAAAAGGT CACCTGGAAT 
ATGGCTGCTC GGGCTTGCAG GGAACAACAT CAAGGCACCC TAGCAAGTGT GTTTACGAAA 
CAGCATATGC AGTGGCTATG GGACTTCAGT GGAAGAAAAT CCTTCTGGAT AGGTTTGAAT 
GACCAAGAGA ATGCAGATCG CTGGGAATGG GATGGAGGTG AACCTGTAAC CTTCACAAAC 
TGGAAGAGAG AGGCCCCCCC ACATTTAAAG AAAAGAAAGA ATTGCGTTTT GGTAAAAAGA 
AGAGGCAAGT GGCAAGCTAA AGACTGCAGG AAGGATAAAG CTCACAACTA CATGTGTGCC 
AGGAAACTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016279747.1
CDS	821..6952
Translation	MNLLGSSGVFLSLLLVLQSTNSSFISVNQGLKVVKGHSAFLSREDLQFSIPKEKDACKVEVVLNEPITQRVGKLTPQVFDCHFLPNEVKYIHNGCPILHEDAVKLRLYRFTETETFTETFILRVYLQEPDCSIIRMSSRVLEVPEFYGLSRAIDKNVLSFDYERRMSLECTIRLSPLETGLPAHGQLVIVEPQQGEPRGDQPHSFFPESQYRKNPGCPSGSCSPELKKIDSLKVSCEEFLLMGLRYQHLHPPSPNVDYISIRLDLTDIRSKSIYKSESMWLPVSIKGAFPNQIPRAAFMPTYILEVDQFILSSVTTSTVDCEDDETPRSLLVFNITKPPLQGYFTHLLDHTKEISSFTWKDLSDLQIAYQPPNSSHTERRNYEVEFEVYDFFFEKSLPITVHISIRTADTNAPRVSWNTGLSLLEGQSRPITWEQFQIVDNDDIKAVRLVTVDGLQHGRLTVRGGKGFIFSVADIQAGTVRYHHDDSDSTKDFVVFRIFDGHHSTRHKFPINILPKDDSPPLLITNVGIELDEGQTVLIQGSMLKASDTDSSDDYIFFNITKPSQAGEIIKKPGPGLIGYPVPGFLQRDLFNGIIYYRHFGGETFEDSFELVLCDSHDPPNLSEPQVVVIHITPVDDELPKEVPGAIRHLVVKETEVAHLTKKHLHFVDTESQDRELLYTITRPPFSSFRYDHSDAGKLFMVDSISKLIKDPAALGLRSFTQHAVNYMKVAYMPPMQDIGPYVQHVQFVFSISNQHGGTLHGICFNITILPVDNEAPEVFTNSLQVEEGGLCIITTEHILISDMDTIQDKIRLSLQRMPLHGMVELNGFPLSQGDKFSWGDLHALKVRYHHDGSEVFHDNILFEATDGTNSAEFVLQVKVLPVNDEPPVLKADLIPTLHCPEGEEVVISSEHVYATDMDSDDTKLMFMIVRQPHHGVVRKSGITVDQFTQGDIDAGTVTYKHTSGEIGMAPCFDTISLVVSDGETGPQDCCYQDPISSPLPPHHSFPVYDLNVTVYPVDNQRPTITTGTQFVVDEGSSAAINVNHLITTDPDTAMDDLQFALVSPPQFGYIENILPSPGFEKSNMGISIASFWWKDMKALHINYVQSRHLRIEPTADHFMIYVTDGKQNSMEIPFSILINPTNDEVPDFVTQNITVSEGQMKELDLSVIKATDLDMPKDHLMFTITHKPQHGLLINGIPRNDIPHYKQLINSHQNHELPVCDFSMELLETGMKLTYIHDDSESLADDFTIQLSDGKHKVLRTISIKILPVNDEKPVLTKKAEIEMNMGETRIISSAILSAMDKDTPREKIYYLFERLPENGQLQLKMVKQASMLKATLPLFARFVLSNGLKTKHGVFKIKLESIDQALPVVTQNKGLRIMERTMALLSPDILQLSDPDTPTENLTFLLAQLPQYGQLYLRGMVLTQHNFTQQEVDSKNVAYQHLGGNSQVDQFTFVATDGTNQGFVVDGRVQNEPVSFIIQVSQLDKKAPRIIQLQCPSQVELLKNGCYGIYITSRVLKASDPDTEDNQIIFKILRGPQYGHLENTTTGEFIQERFSQKDLNSKIILYVINPSSEMNSDNLEFQVTDPSGNTAAPQILELKWSHIHLPQTEYEVCENMNTVPLKITRMGHLMESAFVGVKVNQVSATIGKDFTVSPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKARVKILDSKGGQCLHSHSSGQNQYNSWGKGIWLPLSPGSSSPSISDGIHLEGRHPSSSKEGITHSRGDKLRGFDLTDIPRKRLRTMGNGKTVHPSSIYRNETDIIFRYHGMVGLRVEDDKSAINREKTRGSITNQRLQKKSRNTEPLWADKVDQMPYLHSPIQDQSPSFPKNCTLELKGLFHFEESFQKLYHCNGTSWKPWSPKDQDGNERTCPIGWSHQSSHCYSLITEQKVTWNMAARACREQHQGTLASVFTKQHMQWLWDFSGRKSFWIGLNDQENADRWEWDGGEPVTFTNWKREAPPHLKKRKNCVLVKRRGKWQAKDCRKDKAHNYMCARKL

Target ORF information:

RefSeq Version	XM_016424261.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016424261.1

ORF Insert Sequence:

ATGAACCTGC TTGGATCCAG TGGGGTGTTT CTCAGCCTGC TGCTTGTCCT GCAAAGTACC 
AACTCATCCT TCATCAGCGT CAACCAGGGC TTGAAGGTGG TGAAGGGCCA CTCTGCTTTC 
CTGTCAAGGG AGGACCTGCA GTTTTCCATT CCCAAAGAAA AAGATGCCTG CAAAGTGGAA 
GTTGTGCTAA ATGAACCAAT AACTCAGAGG GTTGGGAAAC TCACTCCACA GGTATTTGAC 
TGTCATTTCC TTCCTAATGA AGTAAAATAC ATTCATAATG GGTGCCCAAT CCTACATGAA 
GATGCTGTTA AACTTAGGCT CTACAGATTT ACAGAAACAG AAACCTTCAC AGAAACTTTT 
ATCCTTCGGG TTTATCTGCA GGAGCCAGAC TGTAGCATCA TCCGGATGAG CTCCAGGGTC 
CTGGAGGTGC CTGAGTTCTA TGGCCTCTCC AGAGCAATTG ATAAGAATGT GCTCAGTTTT 
GATTATGAGA GGAGGATGAG TCTGGAATGC ACCATAAGAC TCTCTCCATT AGAAACTGGC 
CTACCAGCCC ATGGTCAGCT GGTCATTGTG GAACCTCAGC AAGGAGAACC CCGAGGAGAC 
CAGCCCCACA GCTTTTTCCC TGAGTCTCAA TATAGGAAAA ATCCAGGATG CCCGAGTGGA 
AGCTGTTCCC CAGAGTTGAA GAAGATTGAT AGTCTCAAAG TCAGCTGTGA GGAGTTTCTG 
CTAATGGGGT TGCGATATCA GCACCTCCAT CCACCTTCTC CGAATGTCGA TTATATCTCC 
ATCAGACTGG ACCTCACTGA TATCAGGAGT AAATCCATAT ACAAGTCAGA GAGCATGTGG 
CTGCCTGTCT CCATCAAAGG TGCCTTCCCC AATCAGATCC CCAGGGCTGC ATTCATGCCC 
ACCTATATTC TAGAAGTGGA TCAATTCATC CTGAGCTCAG TGACCACTTC TACAGTGGAC 
TGCGAAGATG ATGAGACACC TAGATCTCTG TTGGTTTTCA ACATCACCAA GCCCCCTCTC 
CAGGGCTACT TCACACACCT ATTGGATCAC ACCAAAGAGA TCTCTTCATT CACCTGGAAA 
GATCTAAGTG ATTTGCAAAT TGCCTATCAG CCTCCCAACA GCAGCCACAC AGAGAGAAGG 
AATTATGAGG TGGAGTTTGA AGTGTACGAC TTCTTCTTTG AAAAGAGCTT ACCAATCACA 
GTCCATATTT CTATCAGGAC AGCAGACACG AATGCCCCAA GAGTGTCATG GAACACAGGT 
CTCAGTCTTC TAGAGGGACA GTCAAGGCCC ATCACCTGGG AGCAGTTTCA GATAGTGGAC 
AATGATGACA TCAAGGCAGT TCGCCTGGTC ACTGTTGATG GCTTACAGCA TGGGAGACTT 
ACTGTAAGAG GAGGGAAAGG CTTCATTTTC TCTGTAGCCG ACATTCAGGC TGGGACTGTT 
CGTTACCATC ATGATGACAG TGACTCCACC AAGGATTTTG TGGTCTTCAG GATTTTTGAT 
GGCCATCACA GCACTCGCCA CAAGTTTCCC ATCAATATCC TGCCTAAAGA TGACAGCCCG 
CCTCTCCTCA TAACAAATGT TGGGATTGAA TTAGACGAGG GGCAGACTGT GCTGATCCAA 
GGCTCCATGC TGAAAGCTTC TGACACGGAC TCCAGTGATG ACTACATTTT CTTTAATATC 
ACTAAACCTT CCCAGGCTGG AGAAATCATT AAGAAGCCAG GTCCAGGACT TATTGGTTAC 
CCAGTCCCTG GTTTTCTTCA GAGAGATCTG TTTAATGGGA TTATCTATTA TCGACACTTT 
GGTGGAGAAA CTTTTGAGGA TTCCTTTGAG CTTGTTCTTT GTGACAGCCA CGATCCTCCC 
AACCTCTCAG AACCACAGGT AGTGGTGATA CACATCACCC CAGTGGATGA TGAACTTCCA 
AAAGAGGTTC CTGGAGCGAT TCGGCATTTG GTTGTCAAGG AAACAGAGGT GGCTCATCTC 
ACTAAGAAGC ATCTACATTT TGTGGATACA GAATCTCAGG ATAGGGAGCT TCTCTACACA 
ATAACAAGAC CTCCATTTTC CTCCTTCAGA TATGACCATT CTGATGCTGG GAAATTGTTC 
ATGGTGGATA GCATATCGAA GCTAATCAAG GATCCTGCAG CTCTGGGACT GAGATCATTC 
ACCCAGCATG CTGTAAACTA CATGAAGGTG GCTTACATGC CACCCATGCA AGACATTGGA 
CCCTATGTTC AGCATGTTCA GTTTGTTTTT TCCATCAGTA ACCAGCATGG CGGCACTTTG 
CATGGAATTT GCTTTAATAT TACTATTCTA CCAGTGGATA ATGAGGCTCC TGAGGTGTTC 
ACCAACTCCC TGCAAGTAGA AGAAGGAGGT CTATGTATCA TCACAACAGA ACACATTCTG 
ATTTCAGATA TGGATACAAT ACAAGATAAA ATCAGACTGT CCCTCCAGAG AATGCCACTC 
CATGGAATGG TTGAACTGAA TGGTTTTCCT CTAAGCCAAG GAGACAAATT TTCATGGGGA 
GACCTCCATG CATTAAAAGT TAGGTATCAC CATGATGGAT CAGAGGTTTT TCATGACAAC 
ATACTCTTTG AGGCAACAGA TGGCACAAAT TCTGCAGAAT TTGTCCTTCA GGTCAAGGTT 
TTACCTGTAA ATGATGAACC CCCAGTCCTG AAGGCTGATC TCATTCCCAC ACTGCATTGT 
CCTGAGGGAG AGGAAGTGGT CATCTCTTCA GAACATGTTT ATGCCACTGA CATGGACAGC 
GATGACACCA AACTGATGTT CATGATTGTA CGTCAGCCTC ATCATGGGGT CGTGAGGAAA 
TCTGGGATCA CCGTGGACCA GTTCACCCAG GGAGACATTG ATGCTGGAAC TGTCACATAC 
AAACACACAA GTGGAGAGAT TGGCATGGCC CCTTGTTTTG ACACCATTTC TTTGGTGGTT 
TCGGATGGAG AAACTGGTCC TCAGGACTGT TGCTACCAAG ATCCCATTTC TTCACCACTT 
CCACCTCATC ATTCTTTTCC AGTGTATGAT CTCAATGTGA CTGTGTATCC TGTGGACAAC 
CAAAGACCAA CTATTACAAC TGGTACCCAA TTTGTTGTGG ATGAGGGTTC TTCAGCTGCC 
ATTAATGTTA ACCATTTAAT CACTACTGAC CCCGACACAG CCATGGATGA CTTACAGTTT 
GCTTTGGTTT CTCCTCCACA ATTTGGTTAT ATTGAAAACA TACTTCCTTC TCCAGGTTTT 
GAGAAAAGCA ATATGGGCAT AAGCATAGCT TCATTTTGGT GGAAAGACAT GAAGGCTTTG 
CATATAAACT ATGTGCAGTC CAGACATCTG AGGATAGAGC CAACAGCAGA TCACTTTATG 
ATTTACGTCA CAGATGGAAA ACAGAATTCT ATGGAAATCC CTTTTTCCAT CCTGATTAAC 
CCCACAAATG ATGAAGTTCC TGACTTTGTG ACACAGAACA TCACTGTTTC TGAGGGTCAG 
ATGAAAGAAC TGGATCTTTC AGTCATCAAA GCAACTGACC TAGACATGCC AAAAGATCAT 
TTGATGTTCA CCATTACCCA TAAACCACAG CATGGCCTCC TCATCAATGG GATACCTAGA 
AATGACATCC CTCATTATAA GCAATTAATC AATTCTCACC AGAACCATGA ACTTCCTGTG 
TGTGATTTTT CAATGGAGCT TCTTGAAACT GGAATGAAGT TGACATATAT ACACGATGAC 
TCTGAAAGCC TTGCTGATGA CTTTACAATC CAGTTGTCAG ATGGCAAACA TAAAGTCTTA 
AGAACAATTT CCATAAAGAT CCTGCCAGTT AATGATGAGA AACCAGTGCT GACCAAGAAG 
GCTGAAATAG AAATGAATAT GGGTGAAACT CGCATTATTT CTAGTGCTAT TCTATCAGCT 
ATGGATAAAG ACACACCCAG AGAGAAAATT TACTATTTAT TTGAAAGACT TCCAGAAAAT 
GGGCAGCTTC AGCTCAAGAT GGTGAAACAA GCATCCATGC TGAAGGCTAC CTTGCCTCTC 
TTTGCCAGAT TCGTCTTGAG CAATGGACTA AAGACAAAGC ATGGAGTGTT TAAGATCAAA 
CTGGAGTCTA TTGACCAAGC CTTGCCTGTT GTGACCCAGA ACAAGGGGTT AAGAATAATG 
GAAAGGACCA TGGCGCTCCT CTCTCCAGAC ATCCTTCAAC TTTCTGACCC AGACACTCCA 
ACAGAAAACC TCACCTTCCT CTTGGCCCAG CTCCCCCAGT ATGGACAGCT CTATCTTCGG 
GGGATGGTTC TCACACAACA CAACTTTACA CAACAAGAAG TGGACAGTAA GAATGTGGCC 
TACCAACACC TAGGAGGAAA CTCACAGGTT GACCAGTTTA CCTTTGTAGC TACTGATGGG 
ACAAATCAAG GCTTTGTTGT GGACGGAAGA GTGCAGAATG AACCTGTGTC TTTCATTATT 
CAGGTGTCCC AGTTGGATAA AAAAGCACCA CGCATCATCC AGCTTCAGTG CCCTTCCCAA 
GTGGAGCTTC TGAAAAATGG TTGCTATGGG ATTTACATAA CATCTCGAGT CCTGAAGGCA 
TCTGACCCTG ATACAGAGGA CAACCAAATC ATCTTTAAGA TTTTACGAGG CCCCCAGTAC 
GGACATCTGG AAAATACAAC AACAGGTGAA TTTATTCAGG AGAGATTCAG TCAAAAGGAC 
CTAAACAGTA AAATCATTCT TTATGTCATC AATCCATCTT CAGAAATGAA TTCGGATAAT 
TTGGAGTTTC AAGTCACAGA CCCCAGTGGA AACACCGCTG CCCCTCAAAT TTTGGAACTA 
AAGTGGTCCC ACATTCATTT GCCCCAGACT GAATATGAAG TTTGTGAAAA CATGAACACA 
GTACCACTGA AAATCACTAG GATGGGCCAT TTAATGGAAT CAGCCTTTGT TGGAGTAAAG 
GTTAATCAAG TATCTGCTAC AATTGGAAAA GATTTCACCG TGAGTCCATC AAAGCTGATT 
CAGTTTGATC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA 
GAGGAAGATG ATGAGGTCTT TGAAGTAATT CTCAACTCCC CTGTGAATGC CGTTCTTGGC 
ACGAAGACAA AAGCCAGAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG CCTCCATTCA 
CATTCCTCTG GCCAAAACCA GTACAACTCA TGGGGGAAAG GCATATGGCT CCCACTTTCC 
CCAGGCTCAT CATCACCCTC CATTTCTGAT GGGATTCATT TGGAAGGAAG ACACCCTTCA 
TCTTCCAAAG AGGGGATAAC ACACAGCAGA GGGGACAAGC TTCGGGGCTT TGACTTAACT 
GATATTCCCA GGAAAAGGCT TAGGACAATG GGAAATGGCA AAACAGTTCA CCCATCATCC 
ATTTATAGAA ATGAAACAGA TATAATTTTT AGGTATCATG GTATGGTTGG ATTAAGAGTA 
GAAGATGACA AATCTGCCAT AAATAGAGAG AAGACCAGAG GGTCTATAAC TAATCAGAGG 
CTACAAAAGA AAAGCAGGAA CACAGAACCA CTATGGGCAG ATAAGGTGGA CCAGATGCCC 
TATTTACACT CCCCTATTCA GGACCAGTCA CCATCCTTTC CAAAAAATTG CACATTAGAA 
TTAAAGGGAC TCTTTCATTT TGAGGAAAGC TTCCAAAAAT TATATCATTG CAATGGAACT 
TCTTGGAAGC CTTGGAGTCC AAAAGACCAG GATGGGAATG AAAGAACCTG TCCCATTGGA 
TGGAGCCATC AAAGCAGCCA TTGCTACTCA TTGATTACAG AGCAAAAGGT CACCTGGAAT 
ATGGCTGCTC GGGCTTGCAG GGAACAACAT CAAGGCACCC TAGCAAGTGT GTTTACGAAA 
CAGCATATGC AGTGGCTATG GGACTTCAGT GGAAGAAAAT CCTTCTGGAT AGGTTTGAAT 
GACCAAGAGA ATGCAGATCG CTGGGAATGG GATGGAGGTG AACCTGTAAC CTTCACAAAC 
TGGAAGAGAG AGGCCCCCCC ACATTTAAAG AAAAGAAAGA ATTGCGTTTT GGTAAAAAGA 
AGAGGCAAGT GGCAAGCTAA AGACTGCAGG AAGGATAAAG CTCACAACTA CATGTGTGCC 
AGGAAACTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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FREM1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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