TTBK1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	TTBK1
Entrez Gene ID	100010778
Full Name	tau tubulin kinase 1
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007483955.1	XP_007484017.1	tau-tubulin kinase 1 isoform X2
XM_007483957.2	XP_007484019.1	tau-tubulin kinase 1 isoform X4
XM_007483956.1	XP_007484018.1	tau-tubulin kinase 1 isoform X3
XM_007483952.2	XP_007484014.1	tau-tubulin kinase 1 isoform X1
XM_007483953.2	XP_007484015.1	tau-tubulin kinase 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following TTBK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTBK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc12265	XM_007483952.2 Latest version!	Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMc12265	XM_007483953.2 Latest version!	Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMc12266	XM_007483955.1 Latest version!	Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMc12267	XM_007483956.1 Latest version!	Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMc12268	XM_007483957.2 Latest version!	Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTBK1 ( XM_007483952.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007484014.1 Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMc12265
Clone ID Related Accession (Same CDS sequence)	XM_007483952.2 , XM_007483953.2
Accession Version	XM_007483952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	tau-tubulin kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007483952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGC CCGGGTGGTG GGGCGGTGAT GTCAGGGCTG GTGCTGATGC TGGCGGCGCA 
GTGCATTGTG GGCAGCTCCC CGCTCCGTCG CCGTCGCTGC CGCTGCCGCG GGAGGAAGAT 
CGGGGTCGGG CCGGGCCGGG CCGGGATGGT CGGGGTCGGG AGGCCGCCGC AGCCGCTGCC 
GCTGCAGCCA CCACCACTGC CAGGGGGAGC GGGCTGCGCC AAGCCGCACT GAGCCGCCCC 
CGCCCCCGCC CCGCCCGGGC CCCGGGGGAT GCGCCGTCCG GAGCCGCTGC AGCCGCAGCC 
GCAGCGGAGA CAGAGCAGGT AGATGGCCCA AACCAGGCAG GTGCCACCGA AACCCCTCCC 
TCTGGCTGGC GGATGCAGTG CCTAGCGGCT GCCCTCAGGG AAGAGGCCAA CATGAGTGGG 
GGAGGGGAGC AGGCCGACAT CCTGCCGGCC AACTACGTGG TCAAGGAGCG CTGGAAAGTG 
CTAAAGAAGA TCGGGGGCGG GGGCTTTGGA GAGATCTATG AGGCCATGGA CCTGTTGACC 
CGGGAGAATG TGGCCCTGAA GGTTGAGTCT GCCCAGCAGC CCAAGCAGGT CCTCAAGATG 
GAAGTGGCGG TGCTGAAGAA ACTGCAAGGA AAGGATCATG TTTGCAGGTT TATTGGCTGC 
GGCAGGAATG AGAAGTTCAA TTATGTGGTA ATGCAACTCC AGGGTCGCAA TCTGGCAGAC 
CTGCGCAGGA GTCAGCCCCG GGGCACCTTC ACACTGAGCA CCACATTGAG GCTGGGCAAG 
CAGATCTTGG AGTCCATCGA AGCCATCCAT TCTGTTGGAT TCCTTCATCG GGACATCAAG 
CCTTCGAATT TTGCCATGGG CCGCTTGCCC TCTACCTACA GGAAGTGCTA CATGCTGGAC 
TTTGGGCTGG CACGACAATA TACCAACACC ACAGGGGAGG TCCGACCTCC TCGGAATGTA 
GCTGGGTTTC GGGGGACAGT TCGATATGCT TCAGTCAATG CCCATAAAAA CCGGGAGATG 
GGCCGACACG ATGACCTGTG GTCTCTCTTC TACATGCTGG TGGAATTCGC TGTAGGACAG 
CTCCCCTGGC GTAAGATCAA GGATAAGGAG CAGGTGGGGA TGATCAAAGA GAAATATGAG 
CATCGGATGC TACTGAAACA CATGCCGTCA GAGTTCCATC TCTTCCTGGA TCACATTGCC 
AACCTCGACT ATTTCACCAA GCCCGATTAC CAGTTGATCA TGTCGGTGTT TGAGAACAGC 
ATGAAGGAAC GGGGCATCAC TGAGAATGAG GCCTTTGACT GGGAAAAGGC AGGGACTGAT 
GTCCTCCTTT CTACCAGTAC CTCCACCCCA CCACAGCAGA ACACTCGACA GACAGCAGCA 
ATGTTTGGGG TGGTGAATGT GACCCCTGTG CCTGGGGACC TGCTAAGGGA GAACACAGAG 
GACGTTTTGC AGGGCGAGCA TCTGAGTGAC CAGGAAAATG CACCCCCTCT TCTACCTGGG 
CGGCCATTGG AGGGGCTAGG CTCCATCCCT CATCCTGCTT CCCATCCTGG GGTCCCAGAA 
GCTGAGGTCT GGGAGGAGAC TGATGTCAAC CGCAACAAGC TCAGGATTAA CATCAGCAAA 
ACACAGTGTG TGGTGGAGGA GGATCAGAGT CGTGGAGTGG GGGTCCCAAG CTCCCCAGTA 
CGTGCACCCC CAGACTCCCC AACCACCCCT GTCCGTTCCC TGCGCTATCG AAGGGTCAAC 
AGCCCTGAGT CTGAGCGGCT ATCCACAGCT GATGGGCGGG CTGAGCTACA TGAGAAGAGG 
TCTCGGATGG ACCTTCCGGG TTCCCCTTCC CGCCAGGCGT GCTCGTCCCA ACCTGCCCAG 
ATGCTGTCAG TGGACACAGG TCAGGCGGAC CGGCAGGCCA GTGGGCGCAT GGACGTGTCG 
GCCTCAGTGG AGCAGGAGGC TCTGAGCAAC GCCTTCCGCT CTGTGCCACT GGCGGAGGAA 
GAGGACTTTG ACAGCAAGGA GTGGGTCATC ATCGACAAAG AAACGGAGCT CAAGGACTTC 
CACCCGGGCG CCGAACCCAG CACGTCGGGC ACAACAGACG AGGAGCCGGA GGAGCTGCGG 
CCCCTGCCCG ACGAGGGGGA TGAGCGCAGG CGGCCCGGGG CTGGGGCCGA GTCCGGCCTG 
GGCGCCCCGG TGCGGCCCCG AGGACGCAGC ATGCAGACTG TGGCTGAGGA AGACCTCCGG 
CAGCAGCATC AGTTGCAGGG TGCAGCAGAG CCTGCCCTAT CTCCCACCCC GGGAAGCCCC 
TCCCATTCCT CCCTGCACTC TGGACCCAGG CCTCGGAGGA GGGAGTCAGA CCCCATCGGC 
CCACAGAGAC AGGTGTTCTC ATTGACTTCA CCTTTTGAGG TGAATGGCCT CCCCAGGGCT 
GTGCCCCTCA GCCTGCCCTA CCAGGACTTC AAGAAAGACC TGTCCGAGTA CAGGGAGCGG 
GCTCGCCTCC TGCATAGGGT CCGAACAGTG GACTTCTCCC ATGTTGTCCT TAGCGCTCCT 
TGTCAGTCAG TGACTATTCC CACCCAGCAA GAGGTCAATG GGAGGCGCCA GGAAGAGGAC 
AATGAGGAGG AGGAGGAAGA GGAAGAAGAG GAAGAGGAGG AAGAAGAAGA AGAGGAAGAG 
GAGGAAGAAG AGGAGGAGGA GGAGGTAGAG GAGGAGGAGG AGGAAGAGGA AGAAGAGGAA 
GCAGCCTTGG GGCGGGTATT GGGGCTGAGG AGTGACTCTA GCAGTGAAGT GAGTGAGAAG 
AGCACTGACA GAAGCCAAGA GGGAGCTCCA TCCACATTAC TTGCTGATGA CCAGAAAGAA 
TCCAAGGGCA GGGCGTCCAT GGCTGACGGG GACCTGGAGC CAGAGGAGGG CTCCAAGACA 
CTGGTGCTCT TCTCCCCAGG TGACATGAAG AAGTCACCTG TCACCACCGA CCTGGCTCCC 
GACCCGGACC TAGGCACCCT GGCTGCCCTG ACCCCACAGA ATGAGCGGCC TCACCCCACG 
GGGAGCCAAC TGGATGTGTC TGAGCCAGGG ACGCTGTCCT CTGTCCTCAA GTCTGAACCG 
AAGCCCCCTG GGGCTGGTAC CGGGGTGGGA CCCGCTGTAG GACCAGGGGG AACCGTGGCG 
GCTGCCTCCT CACCCTTCAC CAAAGTCGAA AGGACCTTTG TGCATATTGC TGAGAAAACG 
CATCTCAATG TTATGTCTTC CGGTGGGCAG CCCCTTCGGC CTGAGGAAGC CAGCCCTGGC 
AATGGAGGGC TCGGCCCAGA GGCCATCCTG GAGAAGGGAA GTGTTGAGGA GAGGCCCCCT 
GTGCCCTTAG AGAATGGCAG TATTCTCTCT GGGCCAGATG GGGCAGAGAC GGAGAGTTGT 
GCCCTCTCAG TACTGCCCGG GGAGACACCC TCAGAAGTGG CTGGTGACGC TCTGCCCAAT 
GGCCTTGCTC CCACCAGTGG ACAGCCCCCT GCTATCCCAC CTGAACCCAG TCTACCCTCA 
GAGCCCGGGG TCCCAGAGAA GACAGTTCCC GTGACCCCAG GACCCCAGCC ACTCCTTGCC 
TCCCGACCCC GGAGCCGTAT CCCCATCCTC TTCTCTGAGG AGGACACAGG CTCTGAGTTG 
TCCGCCTCAG TCTCGGCCAA AGAACGATGG TGTAAGCGCA CGCGGCAGCC GGACCTCGCC 
CGCCTGGTGA TGGAGAAGAG GCAGGGCCGC CTCCTGTTTC GGATTGCATC CGGAGCCTCA 
TCCTCGGCCT CATCCAGTGA GGAGCGGCGG CGGGCCTCCG AGACGCTGTC TGGCACGGGA 
TCCGAGGAGG ACACCCATGA CTCAGATGAC TCGGCCCCCA GGAAAGCGGA GCGAGCTGTG 
GGCACACGGA GCCGAATCCC TCGCCCCGTC GGCATCAGGA CACCCACCCC GATCCAACTC 
AAAAGCGGGG GGAGTCCCCA GAGGACGCAA CACCCCAGTG CCCCCAACAG CATGGTCCCA 
TCTCCAGACA CAGCCATCAC CAGCAGGCTC CAGCTACAGA GATCCCCTGG GCCTCCAGCC 
ACTGCTGACC TCCGCGCCAA ACAACCTCCT GTCCACTCAA GCCCTGGCTC TGCCCCAAAC 
CGTGCCAGTC CCCGTCGCCC CAGCCATGCC CCTCCCTCCC GTGGCCCAGC CCTCTCTTCC 
ACCAGACATC TCAGTGCTTC CCCAAGGAGC CAGTCTCTAT CCCGGAGAGA GAGTCCCTCT 
CCCTCCCACC AGGCTAAGCC AGGGGGCTCC CAACCACGGG GAGCCCCCCT GGCCAGGACC 
CAGCCCCAGG TGTCGGGGCC TGGGCAGCCT GAAGGCCCCC CCTCACCGGG GGGCCCCAAG 
AAAGGACCAA GAGGGAAACT CCAGACTTAC AGCCCGGCAA CCAAAGGCAG AGCGGGGGCC 
TCAGGGGCCT CAGAGGGCAA AACTGGGGCC AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007484014.1
CDS	1142..5557
Translation	MSEPGWWGGDVRAGADAGGAVHCGQLPAPSPSLPLPREEDRGRAGPGRDGRGREAAAAAAAAATTTARGSGLRQAALSRPRPRPARAPGDAPSGAAAAAAAAETEQVDGPNQAGATETPPSGWRMQCLAAALREEANMSGGGEQADILPANYVVKERWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLDFGLARQYTNTTGEVRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIANLDYFTKPDYQLIMSVFENSMKERGITENEAFDWEKAGTDVLLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPLLPGRPLEGLGSIPHPASHPGVPEAEVWEETDVNRNKLRINISKTQCVVEEDQSRGVGVPSSPVRAPPDSPTTPVRSLRYRRVNSPESERLSTADGRAELHEKRSRMDLPGSPSRQACSSQPAQMLSVDTGQADRQASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFHPGAEPSTSGTTDEEPEELRPLPDEGDERRRPGAGAESGLGAPVRPRGRSMQTVAEEDLRQQHQLQGAAEPALSPTPGSPSHSSLHSGPRPRRRESDPIGPQRQVFSLTSPFEVNGLPRAVPLSLPYQDFKKDLSEYRERARLLHRVRTVDFSHVVLSAPCQSVTIPTQQEVNGRRQEEDNEEEEEEEEEEEEEEEEEEEEEEEEEEEVEEEEEEEEEEEAALGRVLGLRSDSSSEVSEKSTDRSQEGAPSTLLADDQKESKGRASMADGDLEPEEGSKTLVLFSPGDMKKSPVTTDLAPDPDLGTLAALTPQNERPHPTGSQLDVSEPGTLSSVLKSEPKPPGAGTGVGPAVGPGGTVAAASSPFTKVERTFVHIAEKTHLNVMSSGGQPLRPEEASPGNGGLGPEAILEKGSVEERPPVPLENGSILSGPDGAETESCALSVLPGETPSEVAGDALPNGLAPTSGQPPAIPPEPSLPSEPGVPEKTVPVTPGPQPLLASRPRSRIPILFSEEDTGSELSASVSAKERWCKRTRQPDLARLVMEKRQGRLLFRIASGASSSASSSEERRRASETLSGTGSEEDTHDSDDSAPRKAERAVGTRSRIPRPVGIRTPTPIQLKSGGSPQRTQHPSAPNSMVPSPDTAITSRLQLQRSPGPPATADLRAKQPPVHSSPGSAPNRASPRRPSHAPPSRGPALSSTRHLSASPRSQSLSRRESPSPSHQAKPGGSQPRGAPLARTQPQVSGPGQPEGPPSPGGPKKGPRGKLQTYSPATKGRAGASGASEGKTGAR

Target ORF information:

RefSeq Version	XM_007483952.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007483952.2

ORF Insert Sequence:

ATGTCAGAGC CCGGGTGGTG GGGCGGTGAT GTCAGGGCTG GTGCTGATGC TGGCGGCGCA 
GTGCATTGTG GGCAGCTCCC CGCTCCGTCG CCGTCGCTGC CGCTGCCGCG GGAGGAAGAT 
CGGGGTCGGG CCGGGCCGGG CCGGGATGGT CGGGGTCGGG AGGCCGCCGC AGCCGCTGCC 
GCTGCAGCCA CCACCACTGC CAGGGGGAGC GGGCTGCGCC AAGCCGCACT GAGCCGCCCC 
CGCCCCCGCC CCGCCCGGGC CCCGGGGGAT GCGCCGTCCG GAGCCGCTGC AGCCGCAGCC 
GCAGCGGAGA CAGAGCAGGT AGATGGCCCA AACCAGGCAG GTGCCACCGA AACCCCTCCC 
TCTGGCTGGC GGATGCAGTG CCTAGCGGCT GCCCTCAGGG AAGAGGCCAA CATGAGTGGG 
GGAGGGGAGC AGGCCGACAT CCTGCCGGCC AACTACGTGG TCAAGGAGCG CTGGAAAGTG 
CTAAAGAAGA TCGGGGGCGG GGGCTTTGGA GAGATCTATG AGGCCATGGA CCTGTTGACC 
CGGGAGAATG TGGCCCTGAA GGTTGAGTCT GCCCAGCAGC CCAAGCAGGT CCTCAAGATG 
GAAGTGGCGG TGCTGAAGAA ACTGCAAGGA AAGGATCATG TTTGCAGGTT TATTGGCTGC 
GGCAGGAATG AGAAGTTCAA TTATGTGGTA ATGCAACTCC AGGGTCGCAA TCTGGCAGAC 
CTGCGCAGGA GTCAGCCCCG GGGCACCTTC ACACTGAGCA CCACATTGAG GCTGGGCAAG 
CAGATCTTGG AGTCCATCGA AGCCATCCAT TCTGTTGGAT TCCTTCATCG GGACATCAAG 
CCTTCGAATT TTGCCATGGG CCGCTTGCCC TCTACCTACA GGAAGTGCTA CATGCTGGAC 
TTTGGGCTGG CACGACAATA TACCAACACC ACAGGGGAGG TCCGACCTCC TCGGAATGTA 
GCTGGGTTTC GGGGGACAGT TCGATATGCT TCAGTCAATG CCCATAAAAA CCGGGAGATG 
GGCCGACACG ATGACCTGTG GTCTCTCTTC TACATGCTGG TGGAATTCGC TGTAGGACAG 
CTCCCCTGGC GTAAGATCAA GGATAAGGAG CAGGTGGGGA TGATCAAAGA GAAATATGAG 
CATCGGATGC TACTGAAACA CATGCCGTCA GAGTTCCATC TCTTCCTGGA TCACATTGCC 
AACCTCGACT ATTTCACCAA GCCCGATTAC CAGTTGATCA TGTCGGTGTT TGAGAACAGC 
ATGAAGGAAC GGGGCATCAC TGAGAATGAG GCCTTTGACT GGGAAAAGGC AGGGACTGAT 
GTCCTCCTTT CTACCAGTAC CTCCACCCCA CCACAGCAGA ACACTCGACA GACAGCAGCA 
ATGTTTGGGG TGGTGAATGT GACCCCTGTG CCTGGGGACC TGCTAAGGGA GAACACAGAG 
GACGTTTTGC AGGGCGAGCA TCTGAGTGAC CAGGAAAATG CACCCCCTCT TCTACCTGGG 
CGGCCATTGG AGGGGCTAGG CTCCATCCCT CATCCTGCTT CCCATCCTGG GGTCCCAGAA 
GCTGAGGTCT GGGAGGAGAC TGATGTCAAC CGCAACAAGC TCAGGATTAA CATCAGCAAA 
ACACAGTGTG TGGTGGAGGA GGATCAGAGT CGTGGAGTGG GGGTCCCAAG CTCCCCAGTA 
CGTGCACCCC CAGACTCCCC AACCACCCCT GTCCGTTCCC TGCGCTATCG AAGGGTCAAC 
AGCCCTGAGT CTGAGCGGCT ATCCACAGCT GATGGGCGGG CTGAGCTACA TGAGAAGAGG 
TCTCGGATGG ACCTTCCGGG TTCCCCTTCC CGCCAGGCGT GCTCGTCCCA ACCTGCCCAG 
ATGCTGTCAG TGGACACAGG TCAGGCGGAC CGGCAGGCCA GTGGGCGCAT GGACGTGTCG 
GCCTCAGTGG AGCAGGAGGC TCTGAGCAAC GCCTTCCGCT CTGTGCCACT GGCGGAGGAA 
GAGGACTTTG ACAGCAAGGA GTGGGTCATC ATCGACAAAG AAACGGAGCT CAAGGACTTC 
CACCCGGGCG CCGAACCCAG CACGTCGGGC ACAACAGACG AGGAGCCGGA GGAGCTGCGG 
CCCCTGCCCG ACGAGGGGGA TGAGCGCAGG CGGCCCGGGG CTGGGGCCGA GTCCGGCCTG 
GGCGCCCCGG TGCGGCCCCG AGGACGCAGC ATGCAGACTG TGGCTGAGGA AGACCTCCGG 
CAGCAGCATC AGTTGCAGGG TGCAGCAGAG CCTGCCCTAT CTCCCACCCC GGGAAGCCCC 
TCCCATTCCT CCCTGCACTC TGGACCCAGG CCTCGGAGGA GGGAGTCAGA CCCCATCGGC 
CCACAGAGAC AGGTGTTCTC ATTGACTTCA CCTTTTGAGG TGAATGGCCT CCCCAGGGCT 
GTGCCCCTCA GCCTGCCCTA CCAGGACTTC AAGAAAGACC TGTCCGAGTA CAGGGAGCGG 
GCTCGCCTCC TGCATAGGGT CCGAACAGTG GACTTCTCCC ATGTTGTCCT TAGCGCTCCT 
TGTCAGTCAG TGACTATTCC CACCCAGCAA GAGGTCAATG GGAGGCGCCA GGAAGAGGAC 
AATGAGGAGG AGGAGGAAGA GGAAGAAGAG GAAGAGGAGG AAGAAGAAGA AGAGGAAGAG 
GAGGAAGAAG AGGAGGAGGA GGAGGTAGAG GAGGAGGAGG AGGAAGAGGA AGAAGAGGAA 
GCAGCCTTGG GGCGGGTATT GGGGCTGAGG AGTGACTCTA GCAGTGAAGT GAGTGAGAAG 
AGCACTGACA GAAGCCAAGA GGGAGCTCCA TCCACATTAC TTGCTGATGA CCAGAAAGAA 
TCCAAGGGCA GGGCGTCCAT GGCTGACGGG GACCTGGAGC CAGAGGAGGG CTCCAAGACA 
CTGGTGCTCT TCTCCCCAGG TGACATGAAG AAGTCACCTG TCACCACCGA CCTGGCTCCC 
GACCCGGACC TAGGCACCCT GGCTGCCCTG ACCCCACAGA ATGAGCGGCC TCACCCCACG 
GGGAGCCAAC TGGATGTGTC TGAGCCAGGG ACGCTGTCCT CTGTCCTCAA GTCTGAACCG 
AAGCCCCCTG GGGCTGGTAC CGGGGTGGGA CCCGCTGTAG GACCAGGGGG AACCGTGGCG 
GCTGCCTCCT CACCCTTCAC CAAAGTCGAA AGGACCTTTG TGCATATTGC TGAGAAAACG 
CATCTCAATG TTATGTCTTC CGGTGGGCAG CCCCTTCGGC CTGAGGAAGC CAGCCCTGGC 
AATGGAGGGC TCGGCCCAGA GGCCATCCTG GAGAAGGGAA GTGTTGAGGA GAGGCCCCCT 
GTGCCCTTAG AGAATGGCAG TATTCTCTCT GGGCCAGATG GGGCAGAGAC GGAGAGTTGT 
GCCCTCTCAG TACTGCCCGG GGAGACACCC TCAGAAGTGG CTGGTGACGC TCTGCCCAAT 
GGCCTTGCTC CCACCAGTGG ACAGCCCCCT GCTATCCCAC CTGAACCCAG TCTACCCTCA 
GAGCCCGGGG TCCCAGAGAA GACAGTTCCC GTGACCCCAG GACCCCAGCC ACTCCTTGCC 
TCCCGACCCC GGAGCCGTAT CCCCATCCTC TTCTCTGAGG AGGACACAGG CTCTGAGTTG 
TCCGCCTCAG TCTCGGCCAA AGAACGATGG TGTAAGCGCA CGCGGCAGCC GGACCTCGCC 
CGCCTGGTGA TGGAGAAGAG GCAGGGCCGC CTCCTGTTTC GGATTGCATC CGGAGCCTCA 
TCCTCGGCCT CATCCAGTGA GGAGCGGCGG CGGGCCTCCG AGACGCTGTC TGGCACGGGA 
TCCGAGGAGG ACACCCATGA CTCAGATGAC TCGGCCCCCA GGAAAGCGGA GCGAGCTGTG 
GGCACACGGA GCCGAATCCC TCGCCCCGTC GGCATCAGGA CACCCACCCC GATCCAACTC 
AAAAGCGGGG GGAGTCCCCA GAGGACGCAA CACCCCAGTG CCCCCAACAG CATGGTCCCA 
TCTCCAGACA CAGCCATCAC CAGCAGGCTC CAGCTACAGA GATCCCCTGG GCCTCCAGCC 
ACTGCTGACC TCCGCGCCAA ACAACCTCCT GTCCACTCAA GCCCTGGCTC TGCCCCAAAC 
CGTGCCAGTC CCCGTCGCCC CAGCCATGCC CCTCCCTCCC GTGGCCCAGC CCTCTCTTCC 
ACCAGACATC TCAGTGCTTC CCCAAGGAGC CAGTCTCTAT CCCGGAGAGA GAGTCCCTCT 
CCCTCCCACC AGGCTAAGCC AGGGGGCTCC CAACCACGGG GAGCCCCCCT GGCCAGGACC 
CAGCCCCAGG TGTCGGGGCC TGGGCAGCCT GAAGGCCCCC CCTCACCGGG GGGCCCCAAG 
AAAGGACCAA GAGGGAAACT CCAGACTTAC AGCCCGGCAA CCAAAGGCAG AGCGGGGGCC 
TCAGGGGCCT CAGAGGGCAA AACTGGGGCC AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTBK1 ( XM_007483953.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007484015.1 Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMc12265
Clone ID Related Accession (Same CDS sequence)	XM_007483952.2 , XM_007483953.2
Accession Version	XM_007483953.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	tau-tubulin kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007483953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGC CCGGGTGGTG GGGCGGTGAT GTCAGGGCTG GTGCTGATGC TGGCGGCGCA 
GTGCATTGTG GGCAGCTCCC CGCTCCGTCG CCGTCGCTGC CGCTGCCGCG GGAGGAAGAT 
CGGGGTCGGG CCGGGCCGGG CCGGGATGGT CGGGGTCGGG AGGCCGCCGC AGCCGCTGCC 
GCTGCAGCCA CCACCACTGC CAGGGGGAGC GGGCTGCGCC AAGCCGCACT GAGCCGCCCC 
CGCCCCCGCC CCGCCCGGGC CCCGGGGGAT GCGCCGTCCG GAGCCGCTGC AGCCGCAGCC 
GCAGCGGAGA CAGAGCAGGT AGATGGCCCA AACCAGGCAG GTGCCACCGA AACCCCTCCC 
TCTGGCTGGC GGATGCAGTG CCTAGCGGCT GCCCTCAGGG AAGAGGCCAA CATGAGTGGG 
GGAGGGGAGC AGGCCGACAT CCTGCCGGCC AACTACGTGG TCAAGGAGCG CTGGAAAGTG 
CTAAAGAAGA TCGGGGGCGG GGGCTTTGGA GAGATCTATG AGGCCATGGA CCTGTTGACC 
CGGGAGAATG TGGCCCTGAA GGTTGAGTCT GCCCAGCAGC CCAAGCAGGT CCTCAAGATG 
GAAGTGGCGG TGCTGAAGAA ACTGCAAGGA AAGGATCATG TTTGCAGGTT TATTGGCTGC 
GGCAGGAATG AGAAGTTCAA TTATGTGGTA ATGCAACTCC AGGGTCGCAA TCTGGCAGAC 
CTGCGCAGGA GTCAGCCCCG GGGCACCTTC ACACTGAGCA CCACATTGAG GCTGGGCAAG 
CAGATCTTGG AGTCCATCGA AGCCATCCAT TCTGTTGGAT TCCTTCATCG GGACATCAAG 
CCTTCGAATT TTGCCATGGG CCGCTTGCCC TCTACCTACA GGAAGTGCTA CATGCTGGAC 
TTTGGGCTGG CACGACAATA TACCAACACC ACAGGGGAGG TCCGACCTCC TCGGAATGTA 
GCTGGGTTTC GGGGGACAGT TCGATATGCT TCAGTCAATG CCCATAAAAA CCGGGAGATG 
GGCCGACACG ATGACCTGTG GTCTCTCTTC TACATGCTGG TGGAATTCGC TGTAGGACAG 
CTCCCCTGGC GTAAGATCAA GGATAAGGAG CAGGTGGGGA TGATCAAAGA GAAATATGAG 
CATCGGATGC TACTGAAACA CATGCCGTCA GAGTTCCATC TCTTCCTGGA TCACATTGCC 
AACCTCGACT ATTTCACCAA GCCCGATTAC CAGTTGATCA TGTCGGTGTT TGAGAACAGC 
ATGAAGGAAC GGGGCATCAC TGAGAATGAG GCCTTTGACT GGGAAAAGGC AGGGACTGAT 
GTCCTCCTTT CTACCAGTAC CTCCACCCCA CCACAGCAGA ACACTCGACA GACAGCAGCA 
ATGTTTGGGG TGGTGAATGT GACCCCTGTG CCTGGGGACC TGCTAAGGGA GAACACAGAG 
GACGTTTTGC AGGGCGAGCA TCTGAGTGAC CAGGAAAATG CACCCCCTCT TCTACCTGGG 
CGGCCATTGG AGGGGCTAGG CTCCATCCCT CATCCTGCTT CCCATCCTGG GGTCCCAGAA 
GCTGAGGTCT GGGAGGAGAC TGATGTCAAC CGCAACAAGC TCAGGATTAA CATCAGCAAA 
ACACAGTGTG TGGTGGAGGA GGATCAGAGT CGTGGAGTGG GGGTCCCAAG CTCCCCAGTA 
CGTGCACCCC CAGACTCCCC AACCACCCCT GTCCGTTCCC TGCGCTATCG AAGGGTCAAC 
AGCCCTGAGT CTGAGCGGCT ATCCACAGCT GATGGGCGGG CTGAGCTACA TGAGAAGAGG 
TCTCGGATGG ACCTTCCGGG TTCCCCTTCC CGCCAGGCGT GCTCGTCCCA ACCTGCCCAG 
ATGCTGTCAG TGGACACAGG TCAGGCGGAC CGGCAGGCCA GTGGGCGCAT GGACGTGTCG 
GCCTCAGTGG AGCAGGAGGC TCTGAGCAAC GCCTTCCGCT CTGTGCCACT GGCGGAGGAA 
GAGGACTTTG ACAGCAAGGA GTGGGTCATC ATCGACAAAG AAACGGAGCT CAAGGACTTC 
CACCCGGGCG CCGAACCCAG CACGTCGGGC ACAACAGACG AGGAGCCGGA GGAGCTGCGG 
CCCCTGCCCG ACGAGGGGGA TGAGCGCAGG CGGCCCGGGG CTGGGGCCGA GTCCGGCCTG 
GGCGCCCCGG TGCGGCCCCG AGGACGCAGC ATGCAGACTG TGGCTGAGGA AGACCTCCGG 
CAGCAGCATC AGTTGCAGGG TGCAGCAGAG CCTGCCCTAT CTCCCACCCC GGGAAGCCCC 
TCCCATTCCT CCCTGCACTC TGGACCCAGG CCTCGGAGGA GGGAGTCAGA CCCCATCGGC 
CCACAGAGAC AGGTGTTCTC ATTGACTTCA CCTTTTGAGG TGAATGGCCT CCCCAGGGCT 
GTGCCCCTCA GCCTGCCCTA CCAGGACTTC AAGAAAGACC TGTCCGAGTA CAGGGAGCGG 
GCTCGCCTCC TGCATAGGGT CCGAACAGTG GACTTCTCCC ATGTTGTCCT TAGCGCTCCT 
TGTCAGTCAG TGACTATTCC CACCCAGCAA GAGGTCAATG GGAGGCGCCA GGAAGAGGAC 
AATGAGGAGG AGGAGGAAGA GGAAGAAGAG GAAGAGGAGG AAGAAGAAGA AGAGGAAGAG 
GAGGAAGAAG AGGAGGAGGA GGAGGTAGAG GAGGAGGAGG AGGAAGAGGA AGAAGAGGAA 
GCAGCCTTGG GGCGGGTATT GGGGCTGAGG AGTGACTCTA GCAGTGAAGT GAGTGAGAAG 
AGCACTGACA GAAGCCAAGA GGGAGCTCCA TCCACATTAC TTGCTGATGA CCAGAAAGAA 
TCCAAGGGCA GGGCGTCCAT GGCTGACGGG GACCTGGAGC CAGAGGAGGG CTCCAAGACA 
CTGGTGCTCT TCTCCCCAGG TGACATGAAG AAGTCACCTG TCACCACCGA CCTGGCTCCC 
GACCCGGACC TAGGCACCCT GGCTGCCCTG ACCCCACAGA ATGAGCGGCC TCACCCCACG 
GGGAGCCAAC TGGATGTGTC TGAGCCAGGG ACGCTGTCCT CTGTCCTCAA GTCTGAACCG 
AAGCCCCCTG GGGCTGGTAC CGGGGTGGGA CCCGCTGTAG GACCAGGGGG AACCGTGGCG 
GCTGCCTCCT CACCCTTCAC CAAAGTCGAA AGGACCTTTG TGCATATTGC TGAGAAAACG 
CATCTCAATG TTATGTCTTC CGGTGGGCAG CCCCTTCGGC CTGAGGAAGC CAGCCCTGGC 
AATGGAGGGC TCGGCCCAGA GGCCATCCTG GAGAAGGGAA GTGTTGAGGA GAGGCCCCCT 
GTGCCCTTAG AGAATGGCAG TATTCTCTCT GGGCCAGATG GGGCAGAGAC GGAGAGTTGT 
GCCCTCTCAG TACTGCCCGG GGAGACACCC TCAGAAGTGG CTGGTGACGC TCTGCCCAAT 
GGCCTTGCTC CCACCAGTGG ACAGCCCCCT GCTATCCCAC CTGAACCCAG TCTACCCTCA 
GAGCCCGGGG TCCCAGAGAA GACAGTTCCC GTGACCCCAG GACCCCAGCC ACTCCTTGCC 
TCCCGACCCC GGAGCCGTAT CCCCATCCTC TTCTCTGAGG AGGACACAGG CTCTGAGTTG 
TCCGCCTCAG TCTCGGCCAA AGAACGATGG TGTAAGCGCA CGCGGCAGCC GGACCTCGCC 
CGCCTGGTGA TGGAGAAGAG GCAGGGCCGC CTCCTGTTTC GGATTGCATC CGGAGCCTCA 
TCCTCGGCCT CATCCAGTGA GGAGCGGCGG CGGGCCTCCG AGACGCTGTC TGGCACGGGA 
TCCGAGGAGG ACACCCATGA CTCAGATGAC TCGGCCCCCA GGAAAGCGGA GCGAGCTGTG 
GGCACACGGA GCCGAATCCC TCGCCCCGTC GGCATCAGGA CACCCACCCC GATCCAACTC 
AAAAGCGGGG GGAGTCCCCA GAGGACGCAA CACCCCAGTG CCCCCAACAG CATGGTCCCA 
TCTCCAGACA CAGCCATCAC CAGCAGGCTC CAGCTACAGA GATCCCCTGG GCCTCCAGCC 
ACTGCTGACC TCCGCGCCAA ACAACCTCCT GTCCACTCAA GCCCTGGCTC TGCCCCAAAC 
CGTGCCAGTC CCCGTCGCCC CAGCCATGCC CCTCCCTCCC GTGGCCCAGC CCTCTCTTCC 
ACCAGACATC TCAGTGCTTC CCCAAGGAGC CAGTCTCTAT CCCGGAGAGA GAGTCCCTCT 
CCCTCCCACC AGGCTAAGCC AGGGGGCTCC CAACCACGGG GAGCCCCCCT GGCCAGGACC 
CAGCCCCAGG TGTCGGGGCC TGGGCAGCCT GAAGGCCCCC CCTCACCGGG GGGCCCCAAG 
AAAGGACCAA GAGGGAAACT CCAGACTTAC AGCCCGGCAA CCAAAGGCAG AGCGGGGGCC 
TCAGGGGCCT CAGAGGGCAA AACTGGGGCC AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007484015.1
CDS	1537..5952
Translation	MSEPGWWGGDVRAGADAGGAVHCGQLPAPSPSLPLPREEDRGRAGPGRDGRGREAAAAAAAAATTTARGSGLRQAALSRPRPRPARAPGDAPSGAAAAAAAAETEQVDGPNQAGATETPPSGWRMQCLAAALREEANMSGGGEQADILPANYVVKERWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLDFGLARQYTNTTGEVRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIANLDYFTKPDYQLIMSVFENSMKERGITENEAFDWEKAGTDVLLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPLLPGRPLEGLGSIPHPASHPGVPEAEVWEETDVNRNKLRINISKTQCVVEEDQSRGVGVPSSPVRAPPDSPTTPVRSLRYRRVNSPESERLSTADGRAELHEKRSRMDLPGSPSRQACSSQPAQMLSVDTGQADRQASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFHPGAEPSTSGTTDEEPEELRPLPDEGDERRRPGAGAESGLGAPVRPRGRSMQTVAEEDLRQQHQLQGAAEPALSPTPGSPSHSSLHSGPRPRRRESDPIGPQRQVFSLTSPFEVNGLPRAVPLSLPYQDFKKDLSEYRERARLLHRVRTVDFSHVVLSAPCQSVTIPTQQEVNGRRQEEDNEEEEEEEEEEEEEEEEEEEEEEEEEEEVEEEEEEEEEEEAALGRVLGLRSDSSSEVSEKSTDRSQEGAPSTLLADDQKESKGRASMADGDLEPEEGSKTLVLFSPGDMKKSPVTTDLAPDPDLGTLAALTPQNERPHPTGSQLDVSEPGTLSSVLKSEPKPPGAGTGVGPAVGPGGTVAAASSPFTKVERTFVHIAEKTHLNVMSSGGQPLRPEEASPGNGGLGPEAILEKGSVEERPPVPLENGSILSGPDGAETESCALSVLPGETPSEVAGDALPNGLAPTSGQPPAIPPEPSLPSEPGVPEKTVPVTPGPQPLLASRPRSRIPILFSEEDTGSELSASVSAKERWCKRTRQPDLARLVMEKRQGRLLFRIASGASSSASSSEERRRASETLSGTGSEEDTHDSDDSAPRKAERAVGTRSRIPRPVGIRTPTPIQLKSGGSPQRTQHPSAPNSMVPSPDTAITSRLQLQRSPGPPATADLRAKQPPVHSSPGSAPNRASPRRPSHAPPSRGPALSSTRHLSASPRSQSLSRRESPSPSHQAKPGGSQPRGAPLARTQPQVSGPGQPEGPPSPGGPKKGPRGKLQTYSPATKGRAGASGASEGKTGAR

Target ORF information:

RefSeq Version	XM_007483953.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007483953.2

ORF Insert Sequence:

ATGTCAGAGC CCGGGTGGTG GGGCGGTGAT GTCAGGGCTG GTGCTGATGC TGGCGGCGCA 
GTGCATTGTG GGCAGCTCCC CGCTCCGTCG CCGTCGCTGC CGCTGCCGCG GGAGGAAGAT 
CGGGGTCGGG CCGGGCCGGG CCGGGATGGT CGGGGTCGGG AGGCCGCCGC AGCCGCTGCC 
GCTGCAGCCA CCACCACTGC CAGGGGGAGC GGGCTGCGCC AAGCCGCACT GAGCCGCCCC 
CGCCCCCGCC CCGCCCGGGC CCCGGGGGAT GCGCCGTCCG GAGCCGCTGC AGCCGCAGCC 
GCAGCGGAGA CAGAGCAGGT AGATGGCCCA AACCAGGCAG GTGCCACCGA AACCCCTCCC 
TCTGGCTGGC GGATGCAGTG CCTAGCGGCT GCCCTCAGGG AAGAGGCCAA CATGAGTGGG 
GGAGGGGAGC AGGCCGACAT CCTGCCGGCC AACTACGTGG TCAAGGAGCG CTGGAAAGTG 
CTAAAGAAGA TCGGGGGCGG GGGCTTTGGA GAGATCTATG AGGCCATGGA CCTGTTGACC 
CGGGAGAATG TGGCCCTGAA GGTTGAGTCT GCCCAGCAGC CCAAGCAGGT CCTCAAGATG 
GAAGTGGCGG TGCTGAAGAA ACTGCAAGGA AAGGATCATG TTTGCAGGTT TATTGGCTGC 
GGCAGGAATG AGAAGTTCAA TTATGTGGTA ATGCAACTCC AGGGTCGCAA TCTGGCAGAC 
CTGCGCAGGA GTCAGCCCCG GGGCACCTTC ACACTGAGCA CCACATTGAG GCTGGGCAAG 
CAGATCTTGG AGTCCATCGA AGCCATCCAT TCTGTTGGAT TCCTTCATCG GGACATCAAG 
CCTTCGAATT TTGCCATGGG CCGCTTGCCC TCTACCTACA GGAAGTGCTA CATGCTGGAC 
TTTGGGCTGG CACGACAATA TACCAACACC ACAGGGGAGG TCCGACCTCC TCGGAATGTA 
GCTGGGTTTC GGGGGACAGT TCGATATGCT TCAGTCAATG CCCATAAAAA CCGGGAGATG 
GGCCGACACG ATGACCTGTG GTCTCTCTTC TACATGCTGG TGGAATTCGC TGTAGGACAG 
CTCCCCTGGC GTAAGATCAA GGATAAGGAG CAGGTGGGGA TGATCAAAGA GAAATATGAG 
CATCGGATGC TACTGAAACA CATGCCGTCA GAGTTCCATC TCTTCCTGGA TCACATTGCC 
AACCTCGACT ATTTCACCAA GCCCGATTAC CAGTTGATCA TGTCGGTGTT TGAGAACAGC 
ATGAAGGAAC GGGGCATCAC TGAGAATGAG GCCTTTGACT GGGAAAAGGC AGGGACTGAT 
GTCCTCCTTT CTACCAGTAC CTCCACCCCA CCACAGCAGA ACACTCGACA GACAGCAGCA 
ATGTTTGGGG TGGTGAATGT GACCCCTGTG CCTGGGGACC TGCTAAGGGA GAACACAGAG 
GACGTTTTGC AGGGCGAGCA TCTGAGTGAC CAGGAAAATG CACCCCCTCT TCTACCTGGG 
CGGCCATTGG AGGGGCTAGG CTCCATCCCT CATCCTGCTT CCCATCCTGG GGTCCCAGAA 
GCTGAGGTCT GGGAGGAGAC TGATGTCAAC CGCAACAAGC TCAGGATTAA CATCAGCAAA 
ACACAGTGTG TGGTGGAGGA GGATCAGAGT CGTGGAGTGG GGGTCCCAAG CTCCCCAGTA 
CGTGCACCCC CAGACTCCCC AACCACCCCT GTCCGTTCCC TGCGCTATCG AAGGGTCAAC 
AGCCCTGAGT CTGAGCGGCT ATCCACAGCT GATGGGCGGG CTGAGCTACA TGAGAAGAGG 
TCTCGGATGG ACCTTCCGGG TTCCCCTTCC CGCCAGGCGT GCTCGTCCCA ACCTGCCCAG 
ATGCTGTCAG TGGACACAGG TCAGGCGGAC CGGCAGGCCA GTGGGCGCAT GGACGTGTCG 
GCCTCAGTGG AGCAGGAGGC TCTGAGCAAC GCCTTCCGCT CTGTGCCACT GGCGGAGGAA 
GAGGACTTTG ACAGCAAGGA GTGGGTCATC ATCGACAAAG AAACGGAGCT CAAGGACTTC 
CACCCGGGCG CCGAACCCAG CACGTCGGGC ACAACAGACG AGGAGCCGGA GGAGCTGCGG 
CCCCTGCCCG ACGAGGGGGA TGAGCGCAGG CGGCCCGGGG CTGGGGCCGA GTCCGGCCTG 
GGCGCCCCGG TGCGGCCCCG AGGACGCAGC ATGCAGACTG TGGCTGAGGA AGACCTCCGG 
CAGCAGCATC AGTTGCAGGG TGCAGCAGAG CCTGCCCTAT CTCCCACCCC GGGAAGCCCC 
TCCCATTCCT CCCTGCACTC TGGACCCAGG CCTCGGAGGA GGGAGTCAGA CCCCATCGGC 
CCACAGAGAC AGGTGTTCTC ATTGACTTCA CCTTTTGAGG TGAATGGCCT CCCCAGGGCT 
GTGCCCCTCA GCCTGCCCTA CCAGGACTTC AAGAAAGACC TGTCCGAGTA CAGGGAGCGG 
GCTCGCCTCC TGCATAGGGT CCGAACAGTG GACTTCTCCC ATGTTGTCCT TAGCGCTCCT 
TGTCAGTCAG TGACTATTCC CACCCAGCAA GAGGTCAATG GGAGGCGCCA GGAAGAGGAC 
AATGAGGAGG AGGAGGAAGA GGAAGAAGAG GAAGAGGAGG AAGAAGAAGA AGAGGAAGAG 
GAGGAAGAAG AGGAGGAGGA GGAGGTAGAG GAGGAGGAGG AGGAAGAGGA AGAAGAGGAA 
GCAGCCTTGG GGCGGGTATT GGGGCTGAGG AGTGACTCTA GCAGTGAAGT GAGTGAGAAG 
AGCACTGACA GAAGCCAAGA GGGAGCTCCA TCCACATTAC TTGCTGATGA CCAGAAAGAA 
TCCAAGGGCA GGGCGTCCAT GGCTGACGGG GACCTGGAGC CAGAGGAGGG CTCCAAGACA 
CTGGTGCTCT TCTCCCCAGG TGACATGAAG AAGTCACCTG TCACCACCGA CCTGGCTCCC 
GACCCGGACC TAGGCACCCT GGCTGCCCTG ACCCCACAGA ATGAGCGGCC TCACCCCACG 
GGGAGCCAAC TGGATGTGTC TGAGCCAGGG ACGCTGTCCT CTGTCCTCAA GTCTGAACCG 
AAGCCCCCTG GGGCTGGTAC CGGGGTGGGA CCCGCTGTAG GACCAGGGGG AACCGTGGCG 
GCTGCCTCCT CACCCTTCAC CAAAGTCGAA AGGACCTTTG TGCATATTGC TGAGAAAACG 
CATCTCAATG TTATGTCTTC CGGTGGGCAG CCCCTTCGGC CTGAGGAAGC CAGCCCTGGC 
AATGGAGGGC TCGGCCCAGA GGCCATCCTG GAGAAGGGAA GTGTTGAGGA GAGGCCCCCT 
GTGCCCTTAG AGAATGGCAG TATTCTCTCT GGGCCAGATG GGGCAGAGAC GGAGAGTTGT 
GCCCTCTCAG TACTGCCCGG GGAGACACCC TCAGAAGTGG CTGGTGACGC TCTGCCCAAT 
GGCCTTGCTC CCACCAGTGG ACAGCCCCCT GCTATCCCAC CTGAACCCAG TCTACCCTCA 
GAGCCCGGGG TCCCAGAGAA GACAGTTCCC GTGACCCCAG GACCCCAGCC ACTCCTTGCC 
TCCCGACCCC GGAGCCGTAT CCCCATCCTC TTCTCTGAGG AGGACACAGG CTCTGAGTTG 
TCCGCCTCAG TCTCGGCCAA AGAACGATGG TGTAAGCGCA CGCGGCAGCC GGACCTCGCC 
CGCCTGGTGA TGGAGAAGAG GCAGGGCCGC CTCCTGTTTC GGATTGCATC CGGAGCCTCA 
TCCTCGGCCT CATCCAGTGA GGAGCGGCGG CGGGCCTCCG AGACGCTGTC TGGCACGGGA 
TCCGAGGAGG ACACCCATGA CTCAGATGAC TCGGCCCCCA GGAAAGCGGA GCGAGCTGTG 
GGCACACGGA GCCGAATCCC TCGCCCCGTC GGCATCAGGA CACCCACCCC GATCCAACTC 
AAAAGCGGGG GGAGTCCCCA GAGGACGCAA CACCCCAGTG CCCCCAACAG CATGGTCCCA 
TCTCCAGACA CAGCCATCAC CAGCAGGCTC CAGCTACAGA GATCCCCTGG GCCTCCAGCC 
ACTGCTGACC TCCGCGCCAA ACAACCTCCT GTCCACTCAA GCCCTGGCTC TGCCCCAAAC 
CGTGCCAGTC CCCGTCGCCC CAGCCATGCC CCTCCCTCCC GTGGCCCAGC CCTCTCTTCC 
ACCAGACATC TCAGTGCTTC CCCAAGGAGC CAGTCTCTAT CCCGGAGAGA GAGTCCCTCT 
CCCTCCCACC AGGCTAAGCC AGGGGGCTCC CAACCACGGG GAGCCCCCCT GGCCAGGACC 
CAGCCCCAGG TGTCGGGGCC TGGGCAGCCT GAAGGCCCCC CCTCACCGGG GGGCCCCAAG 
AAAGGACCAA GAGGGAAACT CCAGACTTAC AGCCCGGCAA CCAAAGGCAG AGCGGGGGCC 
TCAGGGGCCT CAGAGGGCAA AACTGGGGCC AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTBK1 ( XM_007483955.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007484017.1 Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X3, mRNA.

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CloneID	OMc12266
Clone ID Related Accession (Same CDS sequence)	XM_007483955.1
Accession Version	XM_007483955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	tau-tubulin kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTATT ACATCCTTCC TACCCCTGTG GCTGTATTAG TGAGTCTATC TTTCCAGGTA 
GATGGCCCAA ACCAGGCAGG TGCCACCGAA ACCCCTCCCT CTGGCTGGCG GATGCAGTGC 
CTAGCGGCTG CCCTCAGGGA AGAGGCCAAC ATGAGTGGGG GAGGGGAGCA GGCCGACATC 
CTGCCGGCCA ACTACGTGGT CAAGGAGCGC TGGAAAGTGC TAAAGAAGAT CGGGGGCGGG 
GGCTTTGGAG AGATCTATGA GGCCATGGAC CTGTTGACCC GGGAGAATGT GGCCCTGAAG 
GTTGAGTCTG CCCAGCAGCC CAAGCAGGTC CTCAAGATGG AAGTGGCGGT GCTGAAGAAA 
CTGCAAGGAA AGGATCATGT TTGCAGGTTT ATTGGCTGCG GCAGGAATGA GAAGTTCAAT 
TATGTGGTAA TGCAACTCCA GGGTCGCAAT CTGGCAGACC TGCGCAGGAG TCAGCCCCGG 
GGCACCTTCA CACTGAGCAC CACATTGAGG CTGGGCAAGC AGATCTTGGA GTCCATCGAA 
GCCATCCATT CTGTTGGATT CCTTCATCGG GACATCAAGC CTTCGAATTT TGCCATGGGC 
CGCTTGCCCT CTACCTACAG GAAGTGCTAC ATGCTGGACT TTGGGCTGGC ACGACAATAT 
ACCAACACCA CAGGGGAGGT CCGACCTCCT CGGAATGTAG CTGGGTTTCG GGGGACAGTT 
CGATATGCTT CAGTCAATGC CCATAAAAAC CGGGAGATGG GCCGACACGA TGACCTGTGG 
TCTCTCTTCT ACATGCTGGT GGAATTCGCT GTAGGACAGC TCCCCTGGCG TAAGATCAAG 
GATAAGGAGC AGGTGGGGAT GATCAAAGAG AAATATGAGC ATCGGATGCT ACTGAAACAC 
ATGCCGTCAG AGTTCCATCT CTTCCTGGAT CACATTGCCA ACCTCGACTA TTTCACCAAG 
CCCGATTACC AGTTGATCAT GTCGGTGTTT GAGAACAGCA TGAAGGAACG GGGCATCACT 
GAGAATGAGG CCTTTGACTG GGAAAAGGCA GGGACTGATG TCCTCCTTTC TACCAGTACC 
TCCACCCCAC CACAGCAGAA CACTCGACAG ACAGCAGCAA TGTTTGGGGT GGTGAATGTG 
ACCCCTGTGC CTGGGGACCT GCTAAGGGAG AACACAGAGG ACGTTTTGCA GGGCGAGCAT 
CTGAGTGACC AGGAAAATGC ACCCCCTCTT CTACCTGGGC GGCCATTGGA GGGGCTAGGC 
TCCATCCCTC ATCCTGCTTC CCATCCTGGG GTCCCAGAAG CTGAGGTCTG GGAGGAGACT 
GATGTCAACC GCAACAAGCT CAGGATTAAC ATCAGCAAAA CACAGTGTGT GGTGGAGGAG 
GATCAGAGTC GTGGAGTGGG GGTCCCAAGC TCCCCAGTAC GTGCACCCCC AGACTCCCCA 
ACCACCCCTG TCCGTTCCCT GCGCTATCGA AGGGTCAACA GCCCTGAGTC TGAGCGGCTA 
TCCACAGCTG ATGGGCGGGC TGAGCTACAT GAGAAGAGGT CTCGGATGGA CCTTCCGGGT 
TCCCCTTCCC GCCAGGCGTG CTCGTCCCAA CCTGCCCAGA TGCTGTCAGT GGACACAGGT 
CAGGCGGACC GGCAGGCCAG TGGGCGCATG GACGTGTCGG CCTCAGTGGA GCAGGAGGCT 
CTGAGCAACG CCTTCCGCTC TGTGCCACTG GCGGAGGAAG AGGACTTTGA CAGCAAGGAG 
TGGGTCATCA TCGACAAAGA AACGGAGCTC AAGGACTTCC ACCCGGGCGC CGAACCCAGC 
ACGTCGGGCA CAACAGACGA GGAGCCGGAG GAGCTGCGGC CCCTGCCCGA CGAGGGGGAT 
GAGCGCAGGC GGCCCGGGGC TGGGGCCGAG TCCGGCCTGG GCGCCCCGGT GCGGCCCCGA 
GGACGCAGCA TGCAGACTGT GGCTGAGGAA GACCTCCGGC AGCAGCATCA GTTGCAGGGT 
GCAGCAGAGC CTGCCCTATC TCCCACCCCG GGAAGCCCCT CCCATTCCTC CCTGCACTCT 
GGACCCAGGC CTCGGAGGAG GGAGTCAGAC CCCATCGGCC CACAGAGACA GGTGTTCTCA 
TTGACTTCAC CTTTTGAGGT GAATGGCCTC CCCAGGGCTG TGCCCCTCAG CCTGCCCTAC 
CAGGACTTCA AGAAAGACCT GTCCGAGTAC AGGGAGCGGG CTCGCCTCCT GCATAGGGTC 
CGAACAGTGG ACTTCTCCCA TGTTGTCCTT AGCGCTCCTT GTCAGTCAGT GACTATTCCC 
ACCCAGCAAG AGGTCAATGG GAGGCGCCAG GAAGAGGACA ATGAGGAGGA GGAGGAAGAG 
GAAGAAGAGG AAGAGGAGGA AGAAGAAGAA GAGGAAGAGG AGGAAGAAGA GGAGGAGGAG 
GAGGTAGAGG AGGAGGAGGA GGAAGAGGAA GAAGAGGAAG CAGCCTTGGG GCGGGTATTG 
GGGCTGAGGA GTGACTCTAG CAGTGAAGTG AGTGAGAAGA GCACTGACAG AAGCCAAGAG 
GGAGCTCCAT CCACATTACT TGCTGATGAC CAGAAAGAAT CCAAGGGCAG GGCGTCCATG 
GCTGACGGGG ACCTGGAGCC AGAGGAGGGC TCCAAGACAC TGGTGCTCTT CTCCCCAGGT 
GACATGAAGA AGTCACCTGT CACCACCGAC CTGGCTCCCG ACCCGGACCT AGGCACCCTG 
GCTGCCCTGA CCCCACAGAA TGAGCGGCCT CACCCCACGG GGAGCCAACT GGATGTGTCT 
GAGCCAGGGA CGCTGTCCTC TGTCCTCAAG TCTGAACCGA AGCCCCCTGG GGCTGGTACC 
GGGGTGGGAC CCGCTGTAGG ACCAGGGGGA ACCGTGGCGG CTGCCTCCTC ACCCTTCACC 
AAAGTCGAAA GGACCTTTGT GCATATTGCT GAGAAAACGC ATCTCAATGT TATGTCTTCC 
GGTGGGCAGC CCCTTCGGCC TGAGGAAGCC AGCCCTGGCA ATGGAGGGCT CGGCCCAGAG 
GCCATCCTGG AGAAGGGAAG TGTTGAGGAG AGGCCCCCTG TGCCCTTAGA GAATGGCAGT 
ATTCTCTCTG GGCCAGATGG GGCAGAGACG GAGAGTTGTG CCCTCTCAGT ACTGCCCGGG 
GAGACACCCT CAGAAGTGGC TGGTGACGCT CTGCCCAATG GCCTTGCTCC CACCAGTGGA 
CAGCCCCCTG CTATCCCACC TGAACCCAGT CTACCCTCAG AGCCCGGGGT CCCAGAGAAG 
ACAGTTCCCG TGACCCCAGG ACCCCAGCCA CTCCTTGCCT CCCGACCCCG GAGCCGTATC 
CCCATCCTCT TCTCTGAGGA GGACACAGGC TCTGAGTTGT CCGCCTCAGT CTCGGCCAAA 
GAACGATGGT GTAAGCGCAC GCGGCAGCCG GACCTCGCCC GCCTGGTGAT GGAGAAGAGG 
CAGGGCCGCC TCCTGTTTCG GATTGCATCC GGAGCCTCAT CCTCGGCCTC ATCCAGTGAG 
GAGCGGCGGC GGGCCTCCGA GACGCTGTCT GGCACGGGAT CCGAGGAGGA CACCCATGAC 
TCAGATGACT CGGCCCCCAG GAAAGCGGAG CGAGCTGTGG GCACACGGAG CCGAATCCCT 
CGCCCCGTCG GCATCAGGAC ACCCACCCCG ATCCAACTCA AAAGCGGGGG GAGTCCCCAG 
AGGACGCAAC ACCCCAGTGC CCCCAACAGC ATGGTCCCAT CTCCAGACAC AGCCATCACC 
AGCAGGCTCC AGCTACAGAG ATCCCCTGGG CCTCCAGCCA CTGCTGACCT CCGCGCCAAA 
CAACCTCCTG TCCACTCAAG CCCTGGCTCT GCCCCAAACC GTGCCAGTCC CCGTCGCCCC 
AGCCATGCCC CTCCCTCCCG TGGCCCAGCC CTCTCTTCCA CCAGACATCT CAGTGCTTCC 
CCAAGGAGCC AGTCTCTATC CCGGAGAGAG AGTCCCTCTC CCTCCCACCA GGCTAAGCCA 
GGGGGCTCCC AACCACGGGG AGCCCCCCTG GCCAGGACCC AGCCCCAGGT GTCGGGGCCT 
GGGCAGCCTG AAGGCCCCCC CTCACCGGGG GGCCCCAAGA AAGGACCAAG AGGGAAACTC 
CAGACTTACA GCCCGGCAAC CAAAGGCAGA GCGGGGGCCT CAGGGGCCTC AGAGGGCAAA 
ACTGGGGCCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007484017.1
CDS	118..4272
Translation	MHYYILPTPVAVLVSLSFQVDGPNQAGATETPPSGWRMQCLAAALREEANMSGGGEQADILPANYVVKERWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLDFGLARQYTNTTGEVRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIANLDYFTKPDYQLIMSVFENSMKERGITENEAFDWEKAGTDVLLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPLLPGRPLEGLGSIPHPASHPGVPEAEVWEETDVNRNKLRINISKTQCVVEEDQSRGVGVPSSPVRAPPDSPTTPVRSLRYRRVNSPESERLSTADGRAELHEKRSRMDLPGSPSRQACSSQPAQMLSVDTGQADRQASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFHPGAEPSTSGTTDEEPEELRPLPDEGDERRRPGAGAESGLGAPVRPRGRSMQTVAEEDLRQQHQLQGAAEPALSPTPGSPSHSSLHSGPRPRRRESDPIGPQRQVFSLTSPFEVNGLPRAVPLSLPYQDFKKDLSEYRERARLLHRVRTVDFSHVVLSAPCQSVTIPTQQEVNGRRQEEDNEEEEEEEEEEEEEEEEEEEEEEEEEEEVEEEEEEEEEEEAALGRVLGLRSDSSSEVSEKSTDRSQEGAPSTLLADDQKESKGRASMADGDLEPEEGSKTLVLFSPGDMKKSPVTTDLAPDPDLGTLAALTPQNERPHPTGSQLDVSEPGTLSSVLKSEPKPPGAGTGVGPAVGPGGTVAAASSPFTKVERTFVHIAEKTHLNVMSSGGQPLRPEEASPGNGGLGPEAILEKGSVEERPPVPLENGSILSGPDGAETESCALSVLPGETPSEVAGDALPNGLAPTSGQPPAIPPEPSLPSEPGVPEKTVPVTPGPQPLLASRPRSRIPILFSEEDTGSELSASVSAKERWCKRTRQPDLARLVMEKRQGRLLFRIASGASSSASSSEERRRASETLSGTGSEEDTHDSDDSAPRKAERAVGTRSRIPRPVGIRTPTPIQLKSGGSPQRTQHPSAPNSMVPSPDTAITSRLQLQRSPGPPATADLRAKQPPVHSSPGSAPNRASPRRPSHAPPSRGPALSSTRHLSASPRSQSLSRRESPSPSHQAKPGGSQPRGAPLARTQPQVSGPGQPEGPPSPGGPKKGPRGKLQTYSPATKGRAGASGASEGKTGAR

Target ORF information:

RefSeq Version	XM_007483955.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007483955.1

ORF Insert Sequence:

ATGCACTATT ACATCCTTCC TACCCCTGTG GCTGTATTAG TGAGTCTATC TTTCCAGGTA 
GATGGCCCAA ACCAGGCAGG TGCCACCGAA ACCCCTCCCT CTGGCTGGCG GATGCAGTGC 
CTAGCGGCTG CCCTCAGGGA AGAGGCCAAC ATGAGTGGGG GAGGGGAGCA GGCCGACATC 
CTGCCGGCCA ACTACGTGGT CAAGGAGCGC TGGAAAGTGC TAAAGAAGAT CGGGGGCGGG 
GGCTTTGGAG AGATCTATGA GGCCATGGAC CTGTTGACCC GGGAGAATGT GGCCCTGAAG 
GTTGAGTCTG CCCAGCAGCC CAAGCAGGTC CTCAAGATGG AAGTGGCGGT GCTGAAGAAA 
CTGCAAGGAA AGGATCATGT TTGCAGGTTT ATTGGCTGCG GCAGGAATGA GAAGTTCAAT 
TATGTGGTAA TGCAACTCCA GGGTCGCAAT CTGGCAGACC TGCGCAGGAG TCAGCCCCGG 
GGCACCTTCA CACTGAGCAC CACATTGAGG CTGGGCAAGC AGATCTTGGA GTCCATCGAA 
GCCATCCATT CTGTTGGATT CCTTCATCGG GACATCAAGC CTTCGAATTT TGCCATGGGC 
CGCTTGCCCT CTACCTACAG GAAGTGCTAC ATGCTGGACT TTGGGCTGGC ACGACAATAT 
ACCAACACCA CAGGGGAGGT CCGACCTCCT CGGAATGTAG CTGGGTTTCG GGGGACAGTT 
CGATATGCTT CAGTCAATGC CCATAAAAAC CGGGAGATGG GCCGACACGA TGACCTGTGG 
TCTCTCTTCT ACATGCTGGT GGAATTCGCT GTAGGACAGC TCCCCTGGCG TAAGATCAAG 
GATAAGGAGC AGGTGGGGAT GATCAAAGAG AAATATGAGC ATCGGATGCT ACTGAAACAC 
ATGCCGTCAG AGTTCCATCT CTTCCTGGAT CACATTGCCA ACCTCGACTA TTTCACCAAG 
CCCGATTACC AGTTGATCAT GTCGGTGTTT GAGAACAGCA TGAAGGAACG GGGCATCACT 
GAGAATGAGG CCTTTGACTG GGAAAAGGCA GGGACTGATG TCCTCCTTTC TACCAGTACC 
TCCACCCCAC CACAGCAGAA CACTCGACAG ACAGCAGCAA TGTTTGGGGT GGTGAATGTG 
ACCCCTGTGC CTGGGGACCT GCTAAGGGAG AACACAGAGG ACGTTTTGCA GGGCGAGCAT 
CTGAGTGACC AGGAAAATGC ACCCCCTCTT CTACCTGGGC GGCCATTGGA GGGGCTAGGC 
TCCATCCCTC ATCCTGCTTC CCATCCTGGG GTCCCAGAAG CTGAGGTCTG GGAGGAGACT 
GATGTCAACC GCAACAAGCT CAGGATTAAC ATCAGCAAAA CACAGTGTGT GGTGGAGGAG 
GATCAGAGTC GTGGAGTGGG GGTCCCAAGC TCCCCAGTAC GTGCACCCCC AGACTCCCCA 
ACCACCCCTG TCCGTTCCCT GCGCTATCGA AGGGTCAACA GCCCTGAGTC TGAGCGGCTA 
TCCACAGCTG ATGGGCGGGC TGAGCTACAT GAGAAGAGGT CTCGGATGGA CCTTCCGGGT 
TCCCCTTCCC GCCAGGCGTG CTCGTCCCAA CCTGCCCAGA TGCTGTCAGT GGACACAGGT 
CAGGCGGACC GGCAGGCCAG TGGGCGCATG GACGTGTCGG CCTCAGTGGA GCAGGAGGCT 
CTGAGCAACG CCTTCCGCTC TGTGCCACTG GCGGAGGAAG AGGACTTTGA CAGCAAGGAG 
TGGGTCATCA TCGACAAAGA AACGGAGCTC AAGGACTTCC ACCCGGGCGC CGAACCCAGC 
ACGTCGGGCA CAACAGACGA GGAGCCGGAG GAGCTGCGGC CCCTGCCCGA CGAGGGGGAT 
GAGCGCAGGC GGCCCGGGGC TGGGGCCGAG TCCGGCCTGG GCGCCCCGGT GCGGCCCCGA 
GGACGCAGCA TGCAGACTGT GGCTGAGGAA GACCTCCGGC AGCAGCATCA GTTGCAGGGT 
GCAGCAGAGC CTGCCCTATC TCCCACCCCG GGAAGCCCCT CCCATTCCTC CCTGCACTCT 
GGACCCAGGC CTCGGAGGAG GGAGTCAGAC CCCATCGGCC CACAGAGACA GGTGTTCTCA 
TTGACTTCAC CTTTTGAGGT GAATGGCCTC CCCAGGGCTG TGCCCCTCAG CCTGCCCTAC 
CAGGACTTCA AGAAAGACCT GTCCGAGTAC AGGGAGCGGG CTCGCCTCCT GCATAGGGTC 
CGAACAGTGG ACTTCTCCCA TGTTGTCCTT AGCGCTCCTT GTCAGTCAGT GACTATTCCC 
ACCCAGCAAG AGGTCAATGG GAGGCGCCAG GAAGAGGACA ATGAGGAGGA GGAGGAAGAG 
GAAGAAGAGG AAGAGGAGGA AGAAGAAGAA GAGGAAGAGG AGGAAGAAGA GGAGGAGGAG 
GAGGTAGAGG AGGAGGAGGA GGAAGAGGAA GAAGAGGAAG CAGCCTTGGG GCGGGTATTG 
GGGCTGAGGA GTGACTCTAG CAGTGAAGTG AGTGAGAAGA GCACTGACAG AAGCCAAGAG 
GGAGCTCCAT CCACATTACT TGCTGATGAC CAGAAAGAAT CCAAGGGCAG GGCGTCCATG 
GCTGACGGGG ACCTGGAGCC AGAGGAGGGC TCCAAGACAC TGGTGCTCTT CTCCCCAGGT 
GACATGAAGA AGTCACCTGT CACCACCGAC CTGGCTCCCG ACCCGGACCT AGGCACCCTG 
GCTGCCCTGA CCCCACAGAA TGAGCGGCCT CACCCCACGG GGAGCCAACT GGATGTGTCT 
GAGCCAGGGA CGCTGTCCTC TGTCCTCAAG TCTGAACCGA AGCCCCCTGG GGCTGGTACC 
GGGGTGGGAC CCGCTGTAGG ACCAGGGGGA ACCGTGGCGG CTGCCTCCTC ACCCTTCACC 
AAAGTCGAAA GGACCTTTGT GCATATTGCT GAGAAAACGC ATCTCAATGT TATGTCTTCC 
GGTGGGCAGC CCCTTCGGCC TGAGGAAGCC AGCCCTGGCA ATGGAGGGCT CGGCCCAGAG 
GCCATCCTGG AGAAGGGAAG TGTTGAGGAG AGGCCCCCTG TGCCCTTAGA GAATGGCAGT 
ATTCTCTCTG GGCCAGATGG GGCAGAGACG GAGAGTTGTG CCCTCTCAGT ACTGCCCGGG 
GAGACACCCT CAGAAGTGGC TGGTGACGCT CTGCCCAATG GCCTTGCTCC CACCAGTGGA 
CAGCCCCCTG CTATCCCACC TGAACCCAGT CTACCCTCAG AGCCCGGGGT CCCAGAGAAG 
ACAGTTCCCG TGACCCCAGG ACCCCAGCCA CTCCTTGCCT CCCGACCCCG GAGCCGTATC 
CCCATCCTCT TCTCTGAGGA GGACACAGGC TCTGAGTTGT CCGCCTCAGT CTCGGCCAAA 
GAACGATGGT GTAAGCGCAC GCGGCAGCCG GACCTCGCCC GCCTGGTGAT GGAGAAGAGG 
CAGGGCCGCC TCCTGTTTCG GATTGCATCC GGAGCCTCAT CCTCGGCCTC ATCCAGTGAG 
GAGCGGCGGC GGGCCTCCGA GACGCTGTCT GGCACGGGAT CCGAGGAGGA CACCCATGAC 
TCAGATGACT CGGCCCCCAG GAAAGCGGAG CGAGCTGTGG GCACACGGAG CCGAATCCCT 
CGCCCCGTCG GCATCAGGAC ACCCACCCCG ATCCAACTCA AAAGCGGGGG GAGTCCCCAG 
AGGACGCAAC ACCCCAGTGC CCCCAACAGC ATGGTCCCAT CTCCAGACAC AGCCATCACC 
AGCAGGCTCC AGCTACAGAG ATCCCCTGGG CCTCCAGCCA CTGCTGACCT CCGCGCCAAA 
CAACCTCCTG TCCACTCAAG CCCTGGCTCT GCCCCAAACC GTGCCAGTCC CCGTCGCCCC 
AGCCATGCCC CTCCCTCCCG TGGCCCAGCC CTCTCTTCCA CCAGACATCT CAGTGCTTCC 
CCAAGGAGCC AGTCTCTATC CCGGAGAGAG AGTCCCTCTC CCTCCCACCA GGCTAAGCCA 
GGGGGCTCCC AACCACGGGG AGCCCCCCTG GCCAGGACCC AGCCCCAGGT GTCGGGGCCT 
GGGCAGCCTG AAGGCCCCCC CTCACCGGGG GGCCCCAAGA AAGGACCAAG AGGGAAACTC 
CAGACTTACA GCCCGGCAAC CAAAGGCAGA GCGGGGGCCT CAGGGGCCTC AGAGGGCAAA 
ACTGGGGCCA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTBK1 ( XM_007483956.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007484018.1 Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X4, mRNA.

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CloneID	OMc12267
Clone ID Related Accession (Same CDS sequence)	XM_007483956.1
Accession Version	XM_007483956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	tau-tubulin kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTGCC TAGCGGCTGC CCTCAGGGAA GAGGCCAACA TGAGTGGGGG AGGGGAGCAG 
GCCGACATCC TGCCGGCCAA CTACGTGGTC AAGGAGCGCT GGAAAGTGCT AAAGAAGATC 
GGGGGCGGGG GCTTTGGAGA GATCTATGAG GCCATGGACC TGTTGACCCG GGAGAATGTG 
GCCCTGAAGG TTGAGTCTGC CCAGCAGCCC AAGCAGGTCC TCAAGATGGA AGTGGCGGTG 
CTGAAGAAAC TGCAAGGAAA GGATCATGTT TGCAGGTTTA TTGGCTGCGG CAGGAATGAG 
AAGTTCAATT ATGTGGTAAT GCAACTCCAG GGTCGCAATC TGGCAGACCT GCGCAGGAGT 
CAGCCCCGGG GCACCTTCAC ACTGAGCACC ACATTGAGGC TGGGCAAGCA GATCTTGGAG 
TCCATCGAAG CCATCCATTC TGTTGGATTC CTTCATCGGG ACATCAAGCC TTCGAATTTT 
GCCATGGGCC GCTTGCCCTC TACCTACAGG AAGTGCTACA TGCTGGACTT TGGGCTGGCA 
CGACAATATA CCAACACCAC AGGGGAGGTC CGACCTCCTC GGAATGTAGC TGGGTTTCGG 
GGGACAGTTC GATATGCTTC AGTCAATGCC CATAAAAACC GGGAGATGGG CCGACACGAT 
GACCTGTGGT CTCTCTTCTA CATGCTGGTG GAATTCGCTG TAGGACAGCT CCCCTGGCGT 
AAGATCAAGG ATAAGGAGCA GGTGGGGATG ATCAAAGAGA AATATGAGCA TCGGATGCTA 
CTGAAACACA TGCCGTCAGA GTTCCATCTC TTCCTGGATC ACATTGCCAA CCTCGACTAT 
TTCACCAAGC CCGATTACCA GTTGATCATG TCGGTGTTTG AGAACAGCAT GAAGGAACGG 
GGCATCACTG AGAATGAGGC CTTTGACTGG GAAAAGGCAG GGACTGATGT CCTCCTTTCT 
ACCAGTACCT CCACCCCACC ACAGCAGAAC ACTCGACAGA CAGCAGCAAT GTTTGGGGTG 
GTGAATGTGA CCCCTGTGCC TGGGGACCTG CTAAGGGAGA ACACAGAGGA CGTTTTGCAG 
GGCGAGCATC TGAGTGACCA GGAAAATGCA CCCCCTCTTC TACCTGGGCG GCCATTGGAG 
GGGCTAGGCT CCATCCCTCA TCCTGCTTCC CATCCTGGGG TCCCAGAAGC TGAGGTCTGG 
GAGGAGACTG ATGTCAACCG CAACAAGCTC AGGATTAACA TCAGCAAAAC ACAGTGTGTG 
GTGGAGGAGG ATCAGAGTCG TGGAGTGGGG GTCCCAAGCT CCCCAGTACG TGCACCCCCA 
GACTCCCCAA CCACCCCTGT CCGTTCCCTG CGCTATCGAA GGGTCAACAG CCCTGAGTCT 
GAGCGGCTAT CCACAGCTGA TGGGCGGGCT GAGCTACATG AGAAGAGGTC TCGGATGGAC 
CTTCCGGGTT CCCCTTCCCG CCAGGCGTGC TCGTCCCAAC CTGCCCAGAT GCTGTCAGTG 
GACACAGGTC AGGCGGACCG GCAGGCCAGT GGGCGCATGG ACGTGTCGGC CTCAGTGGAG 
CAGGAGGCTC TGAGCAACGC CTTCCGCTCT GTGCCACTGG CGGAGGAAGA GGACTTTGAC 
AGCAAGGAGT GGGTCATCAT CGACAAAGAA ACGGAGCTCA AGGACTTCCA CCCGGGCGCC 
GAACCCAGCA CGTCGGGCAC AACAGACGAG GAGCCGGAGG AGCTGCGGCC CCTGCCCGAC 
GAGGGGGATG AGCGCAGGCG GCCCGGGGCT GGGGCCGAGT CCGGCCTGGG CGCCCCGGTG 
CGGCCCCGAG GACGCAGCAT GCAGACTGTG GCTGAGGAAG ACCTCCGGCA GCAGCATCAG 
TTGCAGGGTG CAGCAGAGCC TGCCCTATCT CCCACCCCGG GAAGCCCCTC CCATTCCTCC 
CTGCACTCTG GACCCAGGCC TCGGAGGAGG GAGTCAGACC CCATCGGCCC ACAGAGACAG 
GTGTTCTCAT TGACTTCACC TTTTGAGGTG AATGGCCTCC CCAGGGCTGT GCCCCTCAGC 
CTGCCCTACC AGGACTTCAA GAAAGACCTG TCCGAGTACA GGGAGCGGGC TCGCCTCCTG 
CATAGGGTCC GAACAGTGGA CTTCTCCCAT GTTGTCCTTA GCGCTCCTTG TCAGTCAGTG 
ACTATTCCCA CCCAGCAAGA GGTCAATGGG AGGCGCCAGG AAGAGGACAA TGAGGAGGAG 
GAGGAAGAGG AAGAAGAGGA AGAGGAGGAA GAAGAAGAAG AGGAAGAGGA GGAAGAAGAG 
GAGGAGGAGG AGGTAGAGGA GGAGGAGGAG GAAGAGGAAG AAGAGGAAGC AGCCTTGGGG 
CGGGTATTGG GGCTGAGGAG TGACTCTAGC AGTGAAGTGA GTGAGAAGAG CACTGACAGA 
AGCCAAGAGG GAGCTCCATC CACATTACTT GCTGATGACC AGAAAGAATC CAAGGGCAGG 
GCGTCCATGG CTGACGGGGA CCTGGAGCCA GAGGAGGGCT CCAAGACACT GGTGCTCTTC 
TCCCCAGGTG ACATGAAGAA GTCACCTGTC ACCACCGACC TGGCTCCCGA CCCGGACCTA 
GGCACCCTGG CTGCCCTGAC CCCACAGAAT GAGCGGCCTC ACCCCACGGG GAGCCAACTG 
GATGTGTCTG AGCCAGGGAC GCTGTCCTCT GTCCTCAAGT CTGAACCGAA GCCCCCTGGG 
GCTGGTACCG GGGTGGGACC CGCTGTAGGA CCAGGGGGAA CCGTGGCGGC TGCCTCCTCA 
CCCTTCACCA AAGTCGAAAG GACCTTTGTG CATATTGCTG AGAAAACGCA TCTCAATGTT 
ATGTCTTCCG GTGGGCAGCC CCTTCGGCCT GAGGAAGCCA GCCCTGGCAA TGGAGGGCTC 
GGCCCAGAGG CCATCCTGGA GAAGGGAAGT GTTGAGGAGA GGCCCCCTGT GCCCTTAGAG 
AATGGCAGTA TTCTCTCTGG GCCAGATGGG GCAGAGACGG AGAGTTGTGC CCTCTCAGTA 
CTGCCCGGGG AGACACCCTC AGAAGTGGCT GGTGACGCTC TGCCCAATGG CCTTGCTCCC 
ACCAGTGGAC AGCCCCCTGC TATCCCACCT GAACCCAGTC TACCCTCAGA GCCCGGGGTC 
CCAGAGAAGA CAGTTCCCGT GACCCCAGGA CCCCAGCCAC TCCTTGCCTC CCGACCCCGG 
AGCCGTATCC CCATCCTCTT CTCTGAGGAG GACACAGGCT CTGAGTTGTC CGCCTCAGTC 
TCGGCCAAAG AACGATGGTG TAAGCGCACG CGGCAGCCGG ACCTCGCCCG CCTGGTGATG 
GAGAAGAGGC AGGGCCGCCT CCTGTTTCGG ATTGCATCCG GAGCCTCATC CTCGGCCTCA 
TCCAGTGAGG AGCGGCGGCG GGCCTCCGAG ACGCTGTCTG GCACGGGATC CGAGGAGGAC 
ACCCATGACT CAGATGACTC GGCCCCCAGG AAAGCGGAGC GAGCTGTGGG CACACGGAGC 
CGAATCCCTC GCCCCGTCGG CATCAGGACA CCCACCCCGA TCCAACTCAA AAGCGGGGGG 
AGTCCCCAGA GGACGCAACA CCCCAGTGCC CCCAACAGCA TGGTCCCATC TCCAGACACA 
GCCATCACCA GCAGGCTCCA GCTACAGAGA TCCCCTGGGC CTCCAGCCAC TGCTGACCTC 
CGCGCCAAAC AACCTCCTGT CCACTCAAGC CCTGGCTCTG CCCCAAACCG TGCCAGTCCC 
CGTCGCCCCA GCCATGCCCC TCCCTCCCGT GGCCCAGCCC TCTCTTCCAC CAGACATCTC 
AGTGCTTCCC CAAGGAGCCA GTCTCTATCC CGGAGAGAGA GTCCCTCTCC CTCCCACCAG 
GCTAAGCCAG GGGGCTCCCA ACCACGGGGA GCCCCCCTGG CCAGGACCCA GCCCCAGGTG 
TCGGGGCCTG GGCAGCCTGA AGGCCCCCCC TCACCGGGGG GCCCCAAGAA AGGACCAAGA 
GGGAAACTCC AGACTTACAG CCCGGCAACC AAAGGCAGAG CGGGGGCCTC AGGGGCCTCA 
GAGGGCAAAA CTGGGGCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007484018.1
CDS	119..4162
Translation	MQCLAAALREEANMSGGGEQADILPANYVVKERWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLDFGLARQYTNTTGEVRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIANLDYFTKPDYQLIMSVFENSMKERGITENEAFDWEKAGTDVLLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPLLPGRPLEGLGSIPHPASHPGVPEAEVWEETDVNRNKLRINISKTQCVVEEDQSRGVGVPSSPVRAPPDSPTTPVRSLRYRRVNSPESERLSTADGRAELHEKRSRMDLPGSPSRQACSSQPAQMLSVDTGQADRQASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFHPGAEPSTSGTTDEEPEELRPLPDEGDERRRPGAGAESGLGAPVRPRGRSMQTVAEEDLRQQHQLQGAAEPALSPTPGSPSHSSLHSGPRPRRRESDPIGPQRQVFSLTSPFEVNGLPRAVPLSLPYQDFKKDLSEYRERARLLHRVRTVDFSHVVLSAPCQSVTIPTQQEVNGRRQEEDNEEEEEEEEEEEEEEEEEEEEEEEEEEEVEEEEEEEEEEEAALGRVLGLRSDSSSEVSEKSTDRSQEGAPSTLLADDQKESKGRASMADGDLEPEEGSKTLVLFSPGDMKKSPVTTDLAPDPDLGTLAALTPQNERPHPTGSQLDVSEPGTLSSVLKSEPKPPGAGTGVGPAVGPGGTVAAASSPFTKVERTFVHIAEKTHLNVMSSGGQPLRPEEASPGNGGLGPEAILEKGSVEERPPVPLENGSILSGPDGAETESCALSVLPGETPSEVAGDALPNGLAPTSGQPPAIPPEPSLPSEPGVPEKTVPVTPGPQPLLASRPRSRIPILFSEEDTGSELSASVSAKERWCKRTRQPDLARLVMEKRQGRLLFRIASGASSSASSSEERRRASETLSGTGSEEDTHDSDDSAPRKAERAVGTRSRIPRPVGIRTPTPIQLKSGGSPQRTQHPSAPNSMVPSPDTAITSRLQLQRSPGPPATADLRAKQPPVHSSPGSAPNRASPRRPSHAPPSRGPALSSTRHLSASPRSQSLSRRESPSPSHQAKPGGSQPRGAPLARTQPQVSGPGQPEGPPSPGGPKKGPRGKLQTYSPATKGRAGASGASEGKTGAR

Target ORF information:

RefSeq Version	XM_007483956.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007483956.1

ORF Insert Sequence:

ATGCAGTGCC TAGCGGCTGC CCTCAGGGAA GAGGCCAACA TGAGTGGGGG AGGGGAGCAG 
GCCGACATCC TGCCGGCCAA CTACGTGGTC AAGGAGCGCT GGAAAGTGCT AAAGAAGATC 
GGGGGCGGGG GCTTTGGAGA GATCTATGAG GCCATGGACC TGTTGACCCG GGAGAATGTG 
GCCCTGAAGG TTGAGTCTGC CCAGCAGCCC AAGCAGGTCC TCAAGATGGA AGTGGCGGTG 
CTGAAGAAAC TGCAAGGAAA GGATCATGTT TGCAGGTTTA TTGGCTGCGG CAGGAATGAG 
AAGTTCAATT ATGTGGTAAT GCAACTCCAG GGTCGCAATC TGGCAGACCT GCGCAGGAGT 
CAGCCCCGGG GCACCTTCAC ACTGAGCACC ACATTGAGGC TGGGCAAGCA GATCTTGGAG 
TCCATCGAAG CCATCCATTC TGTTGGATTC CTTCATCGGG ACATCAAGCC TTCGAATTTT 
GCCATGGGCC GCTTGCCCTC TACCTACAGG AAGTGCTACA TGCTGGACTT TGGGCTGGCA 
CGACAATATA CCAACACCAC AGGGGAGGTC CGACCTCCTC GGAATGTAGC TGGGTTTCGG 
GGGACAGTTC GATATGCTTC AGTCAATGCC CATAAAAACC GGGAGATGGG CCGACACGAT 
GACCTGTGGT CTCTCTTCTA CATGCTGGTG GAATTCGCTG TAGGACAGCT CCCCTGGCGT 
AAGATCAAGG ATAAGGAGCA GGTGGGGATG ATCAAAGAGA AATATGAGCA TCGGATGCTA 
CTGAAACACA TGCCGTCAGA GTTCCATCTC TTCCTGGATC ACATTGCCAA CCTCGACTAT 
TTCACCAAGC CCGATTACCA GTTGATCATG TCGGTGTTTG AGAACAGCAT GAAGGAACGG 
GGCATCACTG AGAATGAGGC CTTTGACTGG GAAAAGGCAG GGACTGATGT CCTCCTTTCT 
ACCAGTACCT CCACCCCACC ACAGCAGAAC ACTCGACAGA CAGCAGCAAT GTTTGGGGTG 
GTGAATGTGA CCCCTGTGCC TGGGGACCTG CTAAGGGAGA ACACAGAGGA CGTTTTGCAG 
GGCGAGCATC TGAGTGACCA GGAAAATGCA CCCCCTCTTC TACCTGGGCG GCCATTGGAG 
GGGCTAGGCT CCATCCCTCA TCCTGCTTCC CATCCTGGGG TCCCAGAAGC TGAGGTCTGG 
GAGGAGACTG ATGTCAACCG CAACAAGCTC AGGATTAACA TCAGCAAAAC ACAGTGTGTG 
GTGGAGGAGG ATCAGAGTCG TGGAGTGGGG GTCCCAAGCT CCCCAGTACG TGCACCCCCA 
GACTCCCCAA CCACCCCTGT CCGTTCCCTG CGCTATCGAA GGGTCAACAG CCCTGAGTCT 
GAGCGGCTAT CCACAGCTGA TGGGCGGGCT GAGCTACATG AGAAGAGGTC TCGGATGGAC 
CTTCCGGGTT CCCCTTCCCG CCAGGCGTGC TCGTCCCAAC CTGCCCAGAT GCTGTCAGTG 
GACACAGGTC AGGCGGACCG GCAGGCCAGT GGGCGCATGG ACGTGTCGGC CTCAGTGGAG 
CAGGAGGCTC TGAGCAACGC CTTCCGCTCT GTGCCACTGG CGGAGGAAGA GGACTTTGAC 
AGCAAGGAGT GGGTCATCAT CGACAAAGAA ACGGAGCTCA AGGACTTCCA CCCGGGCGCC 
GAACCCAGCA CGTCGGGCAC AACAGACGAG GAGCCGGAGG AGCTGCGGCC CCTGCCCGAC 
GAGGGGGATG AGCGCAGGCG GCCCGGGGCT GGGGCCGAGT CCGGCCTGGG CGCCCCGGTG 
CGGCCCCGAG GACGCAGCAT GCAGACTGTG GCTGAGGAAG ACCTCCGGCA GCAGCATCAG 
TTGCAGGGTG CAGCAGAGCC TGCCCTATCT CCCACCCCGG GAAGCCCCTC CCATTCCTCC 
CTGCACTCTG GACCCAGGCC TCGGAGGAGG GAGTCAGACC CCATCGGCCC ACAGAGACAG 
GTGTTCTCAT TGACTTCACC TTTTGAGGTG AATGGCCTCC CCAGGGCTGT GCCCCTCAGC 
CTGCCCTACC AGGACTTCAA GAAAGACCTG TCCGAGTACA GGGAGCGGGC TCGCCTCCTG 
CATAGGGTCC GAACAGTGGA CTTCTCCCAT GTTGTCCTTA GCGCTCCTTG TCAGTCAGTG 
ACTATTCCCA CCCAGCAAGA GGTCAATGGG AGGCGCCAGG AAGAGGACAA TGAGGAGGAG 
GAGGAAGAGG AAGAAGAGGA AGAGGAGGAA GAAGAAGAAG AGGAAGAGGA GGAAGAAGAG 
GAGGAGGAGG AGGTAGAGGA GGAGGAGGAG GAAGAGGAAG AAGAGGAAGC AGCCTTGGGG 
CGGGTATTGG GGCTGAGGAG TGACTCTAGC AGTGAAGTGA GTGAGAAGAG CACTGACAGA 
AGCCAAGAGG GAGCTCCATC CACATTACTT GCTGATGACC AGAAAGAATC CAAGGGCAGG 
GCGTCCATGG CTGACGGGGA CCTGGAGCCA GAGGAGGGCT CCAAGACACT GGTGCTCTTC 
TCCCCAGGTG ACATGAAGAA GTCACCTGTC ACCACCGACC TGGCTCCCGA CCCGGACCTA 
GGCACCCTGG CTGCCCTGAC CCCACAGAAT GAGCGGCCTC ACCCCACGGG GAGCCAACTG 
GATGTGTCTG AGCCAGGGAC GCTGTCCTCT GTCCTCAAGT CTGAACCGAA GCCCCCTGGG 
GCTGGTACCG GGGTGGGACC CGCTGTAGGA CCAGGGGGAA CCGTGGCGGC TGCCTCCTCA 
CCCTTCACCA AAGTCGAAAG GACCTTTGTG CATATTGCTG AGAAAACGCA TCTCAATGTT 
ATGTCTTCCG GTGGGCAGCC CCTTCGGCCT GAGGAAGCCA GCCCTGGCAA TGGAGGGCTC 
GGCCCAGAGG CCATCCTGGA GAAGGGAAGT GTTGAGGAGA GGCCCCCTGT GCCCTTAGAG 
AATGGCAGTA TTCTCTCTGG GCCAGATGGG GCAGAGACGG AGAGTTGTGC CCTCTCAGTA 
CTGCCCGGGG AGACACCCTC AGAAGTGGCT GGTGACGCTC TGCCCAATGG CCTTGCTCCC 
ACCAGTGGAC AGCCCCCTGC TATCCCACCT GAACCCAGTC TACCCTCAGA GCCCGGGGTC 
CCAGAGAAGA CAGTTCCCGT GACCCCAGGA CCCCAGCCAC TCCTTGCCTC CCGACCCCGG 
AGCCGTATCC CCATCCTCTT CTCTGAGGAG GACACAGGCT CTGAGTTGTC CGCCTCAGTC 
TCGGCCAAAG AACGATGGTG TAAGCGCACG CGGCAGCCGG ACCTCGCCCG CCTGGTGATG 
GAGAAGAGGC AGGGCCGCCT CCTGTTTCGG ATTGCATCCG GAGCCTCATC CTCGGCCTCA 
TCCAGTGAGG AGCGGCGGCG GGCCTCCGAG ACGCTGTCTG GCACGGGATC CGAGGAGGAC 
ACCCATGACT CAGATGACTC GGCCCCCAGG AAAGCGGAGC GAGCTGTGGG CACACGGAGC 
CGAATCCCTC GCCCCGTCGG CATCAGGACA CCCACCCCGA TCCAACTCAA AAGCGGGGGG 
AGTCCCCAGA GGACGCAACA CCCCAGTGCC CCCAACAGCA TGGTCCCATC TCCAGACACA 
GCCATCACCA GCAGGCTCCA GCTACAGAGA TCCCCTGGGC CTCCAGCCAC TGCTGACCTC 
CGCGCCAAAC AACCTCCTGT CCACTCAAGC CCTGGCTCTG CCCCAAACCG TGCCAGTCCC 
CGTCGCCCCA GCCATGCCCC TCCCTCCCGT GGCCCAGCCC TCTCTTCCAC CAGACATCTC 
AGTGCTTCCC CAAGGAGCCA GTCTCTATCC CGGAGAGAGA GTCCCTCTCC CTCCCACCAG 
GCTAAGCCAG GGGGCTCCCA ACCACGGGGA GCCCCCCTGG CCAGGACCCA GCCCCAGGTG 
TCGGGGCCTG GGCAGCCTGA AGGCCCCCCC TCACCGGGGG GCCCCAAGAA AGGACCAAGA 
GGGAAACTCC AGACTTACAG CCCGGCAACC AAAGGCAGAG CGGGGGCCTC AGGGGCCTCA 
GAGGGCAAAA CTGGGGCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTBK1 ( XM_007483957.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007484019.1 Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X5, mRNA.

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CloneID	OMc12268
Clone ID Related Accession (Same CDS sequence)	XM_007483957.2
Accession Version	XM_007483957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	tau-tubulin kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007483957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGT TGACCCGGGA GAATGTGGCC CTGAAGGTTG AGTCTGCCCA GCAGCCCAAG 
CAGGTCCTCA AGATGGAAGT GGCGGTGCTG AAGAAACTGC AAGGAAAGGA TCATGTTTGC 
AGGTTTATTG GCTGCGGCAG GAATGAGAAG TTCAATTATG TGGTAATGCA ACTCCAGGGT 
CGCAATCTGG CAGACCTGCG CAGGAGTCAG CCCCGGGGCA CCTTCACACT GAGCACCACA 
TTGAGGCTGG GCAAGCAGAT CTTGGAGTCC ATCGAAGCCA TCCATTCTGT TGGATTCCTT 
CATCGGGACA TCAAGCCTTC GAATTTTGCC ATGGGCCGCT TGCCCTCTAC CTACAGGAAG 
TGCTACATGC TGGACTTTGG GCTGGCACGA CAATATACCA ACACCACAGG GGAGGTCCGA 
CCTCCTCGGA ATGTAGCTGG GTTTCGGGGG ACAGTTCGAT ATGCTTCAGT CAATGCCCAT 
AAAAACCGGG AGATGGGCCG ACACGATGAC CTGTGGTCTC TCTTCTACAT GCTGGTGGAA 
TTCGCTGTAG GACAGCTCCC CTGGCGTAAG ATCAAGGATA AGGAGCAGGT GGGGATGATC 
AAAGAGAAAT ATGAGCATCG GATGCTACTG AAACACATGC CGTCAGAGTT CCATCTCTTC 
CTGGATCACA TTGCCAACCT CGACTATTTC ACCAAGCCCG ATTACCAGTT GATCATGTCG 
GTGTTTGAGA ACAGCATGAA GGAACGGGGC ATCACTGAGA ATGAGGCCTT TGACTGGGAA 
AAGGCAGGGA CTGATGTCCT CCTTTCTACC AGTACCTCCA CCCCACCACA GCAGAACACT 
CGACAGACAG CAGCAATGTT TGGGGTGGTG AATGTGACCC CTGTGCCTGG GGACCTGCTA 
AGGGAGAACA CAGAGGACGT TTTGCAGGGC GAGCATCTGA GTGACCAGGA AAATGCACCC 
CCTCTTCTAC CTGGGCGGCC ATTGGAGGGG CTAGGCTCCA TCCCTCATCC TGCTTCCCAT 
CCTGGGGTCC CAGAAGCTGA GGTCTGGGAG GAGACTGATG TCAACCGCAA CAAGCTCAGG 
ATTAACATCA GCAAAACACA GTGTGTGGTG GAGGAGGATC AGAGTCGTGG AGTGGGGGTC 
CCAAGCTCCC CAGTACGTGC ACCCCCAGAC TCCCCAACCA CCCCTGTCCG TTCCCTGCGC 
TATCGAAGGG TCAACAGCCC TGAGTCTGAG CGGCTATCCA CAGCTGATGG GCGGGCTGAG 
CTACATGAGA AGAGGTCTCG GATGGACCTT CCGGGTTCCC CTTCCCGCCA GGCGTGCTCG 
TCCCAACCTG CCCAGATGCT GTCAGTGGAC ACAGGTCAGG CGGACCGGCA GGCCAGTGGG 
CGCATGGACG TGTCGGCCTC AGTGGAGCAG GAGGCTCTGA GCAACGCCTT CCGCTCTGTG 
CCACTGGCGG AGGAAGAGGA CTTTGACAGC AAGGAGTGGG TCATCATCGA CAAAGAAACG 
GAGCTCAAGG ACTTCCACCC GGGCGCCGAA CCCAGCACGT CGGGCACAAC AGACGAGGAG 
CCGGAGGAGC TGCGGCCCCT GCCCGACGAG GGGGATGAGC GCAGGCGGCC CGGGGCTGGG 
GCCGAGTCCG GCCTGGGCGC CCCGGTGCGG CCCCGAGGAC GCAGCATGCA GACTGTGGCT 
GAGGAAGACC TCCGGCAGCA GCATCAGTTG CAGGGTGCAG CAGAGCCTGC CCTATCTCCC 
ACCCCGGGAA GCCCCTCCCA TTCCTCCCTG CACTCTGGAC CCAGGCCTCG GAGGAGGGAG 
TCAGACCCCA TCGGCCCACA GAGACAGGTG TTCTCATTGA CTTCACCTTT TGAGGTGAAT 
GGCCTCCCCA GGGCTGTGCC CCTCAGCCTG CCCTACCAGG ACTTCAAGAA AGACCTGTCC 
GAGTACAGGG AGCGGGCTCG CCTCCTGCAT AGGGTCCGAA CAGTGGACTT CTCCCATGTT 
GTCCTTAGCG CTCCTTGTCA GTCAGTGACT ATTCCCACCC AGCAAGAGGT CAATGGGAGG 
CGCCAGGAAG AGGACAATGA GGAGGAGGAG GAAGAGGAAG AAGAGGAAGA GGAGGAAGAA 
GAAGAAGAGG AAGAGGAGGA AGAAGAGGAG GAGGAGGAGG TAGAGGAGGA GGAGGAGGAA 
GAGGAAGAAG AGGAAGCAGC CTTGGGGCGG GTATTGGGGC TGAGGAGTGA CTCTAGCAGT 
GAAGTGAGTG AGAAGAGCAC TGACAGAAGC CAAGAGGGAG CTCCATCCAC ATTACTTGCT 
GATGACCAGA AAGAATCCAA GGGCAGGGCG TCCATGGCTG ACGGGGACCT GGAGCCAGAG 
GAGGGCTCCA AGACACTGGT GCTCTTCTCC CCAGGTGACA TGAAGAAGTC ACCTGTCACC 
ACCGACCTGG CTCCCGACCC GGACCTAGGC ACCCTGGCTG CCCTGACCCC ACAGAATGAG 
CGGCCTCACC CCACGGGGAG CCAACTGGAT GTGTCTGAGC CAGGGACGCT GTCCTCTGTC 
CTCAAGTCTG AACCGAAGCC CCCTGGGGCT GGTACCGGGG TGGGACCCGC TGTAGGACCA 
GGGGGAACCG TGGCGGCTGC CTCCTCACCC TTCACCAAAG TCGAAAGGAC CTTTGTGCAT 
ATTGCTGAGA AAACGCATCT CAATGTTATG TCTTCCGGTG GGCAGCCCCT TCGGCCTGAG 
GAAGCCAGCC CTGGCAATGG AGGGCTCGGC CCAGAGGCCA TCCTGGAGAA GGGAAGTGTT 
GAGGAGAGGC CCCCTGTGCC CTTAGAGAAT GGCAGTATTC TCTCTGGGCC AGATGGGGCA 
GAGACGGAGA GTTGTGCCCT CTCAGTACTG CCCGGGGAGA CACCCTCAGA AGTGGCTGGT 
GACGCTCTGC CCAATGGCCT TGCTCCCACC AGTGGACAGC CCCCTGCTAT CCCACCTGAA 
CCCAGTCTAC CCTCAGAGCC CGGGGTCCCA GAGAAGACAG TTCCCGTGAC CCCAGGACCC 
CAGCCACTCC TTGCCTCCCG ACCCCGGAGC CGTATCCCCA TCCTCTTCTC TGAGGAGGAC 
ACAGGCTCTG AGTTGTCCGC CTCAGTCTCG GCCAAAGAAC GATGGTGTAA GCGCACGCGG 
CAGCCGGACC TCGCCCGCCT GGTGATGGAG AAGAGGCAGG GCCGCCTCCT GTTTCGGATT 
GCATCCGGAG CCTCATCCTC GGCCTCATCC AGTGAGGAGC GGCGGCGGGC CTCCGAGACG 
CTGTCTGGCA CGGGATCCGA GGAGGACACC CATGACTCAG ATGACTCGGC CCCCAGGAAA 
GCGGAGCGAG CTGTGGGCAC ACGGAGCCGA ATCCCTCGCC CCGTCGGCAT CAGGACACCC 
ACCCCGATCC AACTCAAAAG CGGGGGGAGT CCCCAGAGGA CGCAACACCC CAGTGCCCCC 
AACAGCATGG TCCCATCTCC AGACACAGCC ATCACCAGCA GGCTCCAGCT ACAGAGATCC 
CCTGGGCCTC CAGCCACTGC TGACCTCCGC GCCAAACAAC CTCCTGTCCA CTCAAGCCCT 
GGCTCTGCCC CAAACCGTGC CAGTCCCCGT CGCCCCAGCC ATGCCCCTCC CTCCCGTGGC 
CCAGCCCTCT CTTCCACCAG ACATCTCAGT GCTTCCCCAA GGAGCCAGTC TCTATCCCGG 
AGAGAGAGTC CCTCTCCCTC CCACCAGGCT AAGCCAGGGG GCTCCCAACC ACGGGGAGCC 
CCCCTGGCCA GGACCCAGCC CCAGGTGTCG GGGCCTGGGC AGCCTGAAGG CCCCCCCTCA 
CCGGGGGGCC CCAAGAAAGG ACCAAGAGGG AAACTCCAGA CTTACAGCCC GGCAACCAAA 
GGCAGAGCGG GGGCCTCAGG GGCCTCAGAG GGCAAAACTG GGGCCAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007484019.1
CDS	65..3955
Translation	MDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLDFGLARQYTNTTGEVRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIANLDYFTKPDYQLIMSVFENSMKERGITENEAFDWEKAGTDVLLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPLLPGRPLEGLGSIPHPASHPGVPEAEVWEETDVNRNKLRINISKTQCVVEEDQSRGVGVPSSPVRAPPDSPTTPVRSLRYRRVNSPESERLSTADGRAELHEKRSRMDLPGSPSRQACSSQPAQMLSVDTGQADRQASGRMDVSASVEQEALSNAFRSVPLAEEEDFDSKEWVIIDKETELKDFHPGAEPSTSGTTDEEPEELRPLPDEGDERRRPGAGAESGLGAPVRPRGRSMQTVAEEDLRQQHQLQGAAEPALSPTPGSPSHSSLHSGPRPRRRESDPIGPQRQVFSLTSPFEVNGLPRAVPLSLPYQDFKKDLSEYRERARLLHRVRTVDFSHVVLSAPCQSVTIPTQQEVNGRRQEEDNEEEEEEEEEEEEEEEEEEEEEEEEEEEVEEEEEEEEEEEAALGRVLGLRSDSSSEVSEKSTDRSQEGAPSTLLADDQKESKGRASMADGDLEPEEGSKTLVLFSPGDMKKSPVTTDLAPDPDLGTLAALTPQNERPHPTGSQLDVSEPGTLSSVLKSEPKPPGAGTGVGPAVGPGGTVAAASSPFTKVERTFVHIAEKTHLNVMSSGGQPLRPEEASPGNGGLGPEAILEKGSVEERPPVPLENGSILSGPDGAETESCALSVLPGETPSEVAGDALPNGLAPTSGQPPAIPPEPSLPSEPGVPEKTVPVTPGPQPLLASRPRSRIPILFSEEDTGSELSASVSAKERWCKRTRQPDLARLVMEKRQGRLLFRIASGASSSASSSEERRRASETLSGTGSEEDTHDSDDSAPRKAERAVGTRSRIPRPVGIRTPTPIQLKSGGSPQRTQHPSAPNSMVPSPDTAITSRLQLQRSPGPPATADLRAKQPPVHSSPGSAPNRASPRRPSHAPPSRGPALSSTRHLSASPRSQSLSRRESPSPSHQAKPGGSQPRGAPLARTQPQVSGPGQPEGPPSPGGPKKGPRGKLQTYSPATKGRAGASGASEGKTGAR

Target ORF information:

RefSeq Version	XM_007483957.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica tau tubulin kinase 1 (TTBK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007483957.2

ORF Insert Sequence:

ATGGACCTGT TGACCCGGGA GAATGTGGCC CTGAAGGTTG AGTCTGCCCA GCAGCCCAAG 
CAGGTCCTCA AGATGGAAGT GGCGGTGCTG AAGAAACTGC AAGGAAAGGA TCATGTTTGC 
AGGTTTATTG GCTGCGGCAG GAATGAGAAG TTCAATTATG TGGTAATGCA ACTCCAGGGT 
CGCAATCTGG CAGACCTGCG CAGGAGTCAG CCCCGGGGCA CCTTCACACT GAGCACCACA 
TTGAGGCTGG GCAAGCAGAT CTTGGAGTCC ATCGAAGCCA TCCATTCTGT TGGATTCCTT 
CATCGGGACA TCAAGCCTTC GAATTTTGCC ATGGGCCGCT TGCCCTCTAC CTACAGGAAG 
TGCTACATGC TGGACTTTGG GCTGGCACGA CAATATACCA ACACCACAGG GGAGGTCCGA 
CCTCCTCGGA ATGTAGCTGG GTTTCGGGGG ACAGTTCGAT ATGCTTCAGT CAATGCCCAT 
AAAAACCGGG AGATGGGCCG ACACGATGAC CTGTGGTCTC TCTTCTACAT GCTGGTGGAA 
TTCGCTGTAG GACAGCTCCC CTGGCGTAAG ATCAAGGATA AGGAGCAGGT GGGGATGATC 
AAAGAGAAAT ATGAGCATCG GATGCTACTG AAACACATGC CGTCAGAGTT CCATCTCTTC 
CTGGATCACA TTGCCAACCT CGACTATTTC ACCAAGCCCG ATTACCAGTT GATCATGTCG 
GTGTTTGAGA ACAGCATGAA GGAACGGGGC ATCACTGAGA ATGAGGCCTT TGACTGGGAA 
AAGGCAGGGA CTGATGTCCT CCTTTCTACC AGTACCTCCA CCCCACCACA GCAGAACACT 
CGACAGACAG CAGCAATGTT TGGGGTGGTG AATGTGACCC CTGTGCCTGG GGACCTGCTA 
AGGGAGAACA CAGAGGACGT TTTGCAGGGC GAGCATCTGA GTGACCAGGA AAATGCACCC 
CCTCTTCTAC CTGGGCGGCC ATTGGAGGGG CTAGGCTCCA TCCCTCATCC TGCTTCCCAT 
CCTGGGGTCC CAGAAGCTGA GGTCTGGGAG GAGACTGATG TCAACCGCAA CAAGCTCAGG 
ATTAACATCA GCAAAACACA GTGTGTGGTG GAGGAGGATC AGAGTCGTGG AGTGGGGGTC 
CCAAGCTCCC CAGTACGTGC ACCCCCAGAC TCCCCAACCA CCCCTGTCCG TTCCCTGCGC 
TATCGAAGGG TCAACAGCCC TGAGTCTGAG CGGCTATCCA CAGCTGATGG GCGGGCTGAG 
CTACATGAGA AGAGGTCTCG GATGGACCTT CCGGGTTCCC CTTCCCGCCA GGCGTGCTCG 
TCCCAACCTG CCCAGATGCT GTCAGTGGAC ACAGGTCAGG CGGACCGGCA GGCCAGTGGG 
CGCATGGACG TGTCGGCCTC AGTGGAGCAG GAGGCTCTGA GCAACGCCTT CCGCTCTGTG 
CCACTGGCGG AGGAAGAGGA CTTTGACAGC AAGGAGTGGG TCATCATCGA CAAAGAAACG 
GAGCTCAAGG ACTTCCACCC GGGCGCCGAA CCCAGCACGT CGGGCACAAC AGACGAGGAG 
CCGGAGGAGC TGCGGCCCCT GCCCGACGAG GGGGATGAGC GCAGGCGGCC CGGGGCTGGG 
GCCGAGTCCG GCCTGGGCGC CCCGGTGCGG CCCCGAGGAC GCAGCATGCA GACTGTGGCT 
GAGGAAGACC TCCGGCAGCA GCATCAGTTG CAGGGTGCAG CAGAGCCTGC CCTATCTCCC 
ACCCCGGGAA GCCCCTCCCA TTCCTCCCTG CACTCTGGAC CCAGGCCTCG GAGGAGGGAG 
TCAGACCCCA TCGGCCCACA GAGACAGGTG TTCTCATTGA CTTCACCTTT TGAGGTGAAT 
GGCCTCCCCA GGGCTGTGCC CCTCAGCCTG CCCTACCAGG ACTTCAAGAA AGACCTGTCC 
GAGTACAGGG AGCGGGCTCG CCTCCTGCAT AGGGTCCGAA CAGTGGACTT CTCCCATGTT 
GTCCTTAGCG CTCCTTGTCA GTCAGTGACT ATTCCCACCC AGCAAGAGGT CAATGGGAGG 
CGCCAGGAAG AGGACAATGA GGAGGAGGAG GAAGAGGAAG AAGAGGAAGA GGAGGAAGAA 
GAAGAAGAGG AAGAGGAGGA AGAAGAGGAG GAGGAGGAGG TAGAGGAGGA GGAGGAGGAA 
GAGGAAGAAG AGGAAGCAGC CTTGGGGCGG GTATTGGGGC TGAGGAGTGA CTCTAGCAGT 
GAAGTGAGTG AGAAGAGCAC TGACAGAAGC CAAGAGGGAG CTCCATCCAC ATTACTTGCT 
GATGACCAGA AAGAATCCAA GGGCAGGGCG TCCATGGCTG ACGGGGACCT GGAGCCAGAG 
GAGGGCTCCA AGACACTGGT GCTCTTCTCC CCAGGTGACA TGAAGAAGTC ACCTGTCACC 
ACCGACCTGG CTCCCGACCC GGACCTAGGC ACCCTGGCTG CCCTGACCCC ACAGAATGAG 
CGGCCTCACC CCACGGGGAG CCAACTGGAT GTGTCTGAGC CAGGGACGCT GTCCTCTGTC 
CTCAAGTCTG AACCGAAGCC CCCTGGGGCT GGTACCGGGG TGGGACCCGC TGTAGGACCA 
GGGGGAACCG TGGCGGCTGC CTCCTCACCC TTCACCAAAG TCGAAAGGAC CTTTGTGCAT 
ATTGCTGAGA AAACGCATCT CAATGTTATG TCTTCCGGTG GGCAGCCCCT TCGGCCTGAG 
GAAGCCAGCC CTGGCAATGG AGGGCTCGGC CCAGAGGCCA TCCTGGAGAA GGGAAGTGTT 
GAGGAGAGGC CCCCTGTGCC CTTAGAGAAT GGCAGTATTC TCTCTGGGCC AGATGGGGCA 
GAGACGGAGA GTTGTGCCCT CTCAGTACTG CCCGGGGAGA CACCCTCAGA AGTGGCTGGT 
GACGCTCTGC CCAATGGCCT TGCTCCCACC AGTGGACAGC CCCCTGCTAT CCCACCTGAA 
CCCAGTCTAC CCTCAGAGCC CGGGGTCCCA GAGAAGACAG TTCCCGTGAC CCCAGGACCC 
CAGCCACTCC TTGCCTCCCG ACCCCGGAGC CGTATCCCCA TCCTCTTCTC TGAGGAGGAC 
ACAGGCTCTG AGTTGTCCGC CTCAGTCTCG GCCAAAGAAC GATGGTGTAA GCGCACGCGG 
CAGCCGGACC TCGCCCGCCT GGTGATGGAG AAGAGGCAGG GCCGCCTCCT GTTTCGGATT 
GCATCCGGAG CCTCATCCTC GGCCTCATCC AGTGAGGAGC GGCGGCGGGC CTCCGAGACG 
CTGTCTGGCA CGGGATCCGA GGAGGACACC CATGACTCAG ATGACTCGGC CCCCAGGAAA 
GCGGAGCGAG CTGTGGGCAC ACGGAGCCGA ATCCCTCGCC CCGTCGGCAT CAGGACACCC 
ACCCCGATCC AACTCAAAAG CGGGGGGAGT CCCCAGAGGA CGCAACACCC CAGTGCCCCC 
AACAGCATGG TCCCATCTCC AGACACAGCC ATCACCAGCA GGCTCCAGCT ACAGAGATCC 
CCTGGGCCTC CAGCCACTGC TGACCTCCGC GCCAAACAAC CTCCTGTCCA CTCAAGCCCT 
GGCTCTGCCC CAAACCGTGC CAGTCCCCGT CGCCCCAGCC ATGCCCCTCC CTCCCGTGGC 
CCAGCCCTCT CTTCCACCAG ACATCTCAGT GCTTCCCCAA GGAGCCAGTC TCTATCCCGG 
AGAGAGAGTC CCTCTCCCTC CCACCAGGCT AAGCCAGGGG GCTCCCAACC ACGGGGAGCC 
CCCCTGGCCA GGACCCAGCC CCAGGTGTCG GGGCCTGGGC AGCCTGAAGG CCCCCCCTCA 
CCGGGGGGCC CCAAGAAAGG ACCAAGAGGG AAACTCCAGA CTTACAGCCC GGCAACCAAA 
GGCAGAGCGG GGGCCTCAGG GGCCTCAGAG GGCAAAACTG GGGCCAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TTBK1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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