Ralgapa1 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ralgapa1
Entrez Gene ID	101842372
Full Name	Ral GTPase activating protein catalytic alpha subunit 1
Gene Type	protein-coding
Organism	Mesocricetus auratus(golden hamster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013120839.1	XP_012976293.1	ral GTPase-activating protein subunit alpha-1
XM_021233224.1	XP_021088883.1	ral GTPase-activating protein subunit alpha-1 isoform X4
XM_021233220.1	XP_021088879.1	ral GTPase-activating protein subunit alpha-1 isoform X1
XM_021233221.1	XP_021088880.1	ral GTPase-activating protein subunit alpha-1 isoform X2
XM_021233222.1	XP_021088881.1	ral GTPase-activating protein subunit alpha-1 isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Transcriptome sequencing and development of an expression microarray platform for liver infection in adenovirus type 5-infected Syrian golden hamsters.
Ying B, Toth K, Spencer JF, Aurora R, Wold WS
Virology485305-12(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ralgapa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ralgapa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe134485	XM_013120839.1 Latest version!	Mesocricetus auratus Ral GTPase activating protein, alpha subunit 1 (catalytic) (Ralgapa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMe252639	XM_021233220.1 Latest version!	Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMe252640	XM_021233221.1 Latest version!	Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMe252641	XM_021233222.1 Latest version!	Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMe252642	XM_021233224.1 Latest version!	Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ralgapa1 ( XM_013120839.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012976293.1 Mesocricetus auratus Ral GTPase activating protein, alpha subunit 1 (catalytic) (Ralgapa1), mRNA.

Price & Availability↑

CloneID	OMe134485
Clone ID Related Accession (Same CDS sequence)	XM_013120839.1
Accession Version	XM_013120839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	ral GTPase-activating protein subunit alpha-1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801703.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT 
ATTGACCTTA AGCAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGCC ACAAGTCTCA GAGAGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AACTGCTTCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCGCTTCAG 
GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG ACTATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TCCCCCCGCA GTCAGGAGAT 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGAAAATG GGACCAATAT TTCTGAGCAT GTTCGAAATT CTAGTTGGAC CAAAAATGGT 
TCCTACCAAG ATGCTTTTCA TGCCTCTGAA GAAGCCACAG AACAAAATAT TCAAGCTGGT 
ACGCAGGCAG TTTTGCAGGT GTTCATTATA AACTCATCAA ACATATTTCT TCTTGAACCT 
GCCAATGAAA TAAAAAATCT TTTGGATGAG CACACAGATA TGTGTAAACG TATTCTTAAC 
ATTTATCGAT ACATGGTTGT GCAAGTATCA ATGGACAAAA AGACCTGGGA ACAGATGCTG 
CTCGTGCTGC TCAGAGTCAC AGAGTCTGTA CTGAAGATGT CATCACAAGC TTTTCTACAG 
TTCCAGGGGA AAAAAAACAT GACCTTGGCA GGTCGACTTG CAGGACCACT TTTCCAGACT 
CTCATTGTTG CCTGGATCAA AGCAAATCTA AATGTGTACA TCTCTCGAGA ACTTTGGGAT 
GACTTACTTT CAGTATTGTC ATCGTTGACC TATTGGGAAG AATTGGCCAC TGAGTGGTCT 
CTGACCATGG AGACGCTAAC TAAAGTTTTA GCTAGGAATT TATATAGTTT GGATCTCAGT 
GATTTGCCGT TGGATAAGCT GAGTGAACAG AAACAAAAAA AGCACAAAGG GAAAGGAGTT 
GGACATGAAT TTCAGAAAGT TTCAGTTGAC AAGTCATTTT CTAGAGGATG GAGTCGTGAT 
CAGCCTGGCC AGGCACCCAT GAGACAAAGA AGTGCAACAA CTACTGGGTC CCCAGGAACT 
GAAAAGGCAA GGAGTATAGT ACGACAAAAA ACTGTTGCCA TGAGAAGCCG ATCCATTGGT 
GAATGTGCTC TGCCATCGGC CTATATTCGC AGTGCTAAAA GTGCTCCTGT TCTGATCCAT 
ACTTCCAAAC CCTTCTTGCC TGATATTGTT CTCACTCCCC TTTCTGATGA GCTTTCAGAT 
ATTGATGATG CTCAAATCCT TCCTCGCTCA ACCAGAGTCA GACATTTTTC ACAGAGCGAA 
GACACTGGAA ATGAAGTGTT TGGAGCTCTG AGTGAGGAAC AGCCATTGCC TCGAAGCAGC 
AGCACTTCTG ACCTCGTGGA GCCGTTCACT GTTGAGCGAG CCAAAGGTGC AGTTCCTGTC 
GTTGACACTT CATCCCGTCA TGCTCCGAGC TTGCAGACTT CCACAGAGGC GTCTTCAGTA 
ACTAGATCCA CTGAGAGCTG CAGCACTGAT ACTCGTAGTA GAGAGTCTTC TCTGGAAGTT 
GGTGATAGCA TATATGACCA TCTTTGTCAT TTAATAGGTC CAGTAGAACT TGCAGATGCA 
GCTTTCGAAC AAATCCAGTA CATTGACCTT GAAGGAGATG ATGATCTTCT TTCTTCCCTG 
AAAGAATACT TTAAGGAAAA CCAGGAAAAT CATAGTAACA ATGAGCTAGG TAAAGATACA 
GCTTCTCAGA AGGTGATTAT TGCAGTAACT AGAGATGAAA TACCATCTTT AGATAAACTA 
GACTACATAG GCCAGGAAAT AAAATCAGAA AATCTCATGT CTTATGTTGG GACTCCTGAA 
AATACACCAT TTCAAAAACA ACCAAGCTCA GCTGTCTTCA CGAGTACTGT TGCACCTAAG 
CAGTTAGTTA GTTTTTCAAC AACACAAGCT GCTGAAAAAC CTATGCAACT AAATCTTGAT 
GAACAGTCAT CGGATCCTAG TTCAGATAGC CCTTGTGATA AAGAAAAAAA GAAACATCTG 
TATAGACAAA CAGCCACAGA AGTAGATGCC TGTGTAGATA TGACACTAGG TGAAAAGCTT 
AAGAGTATAC AAGTTAATGA AAAAATCACC CCATATATAA TGCATGCCAA GAAAACTGCA 
CTAAAAGCAC CAGTGAACCG CAGAATGCCT CATGTTGCAA ACACTAGCAA GCTTTCTCCA 
ACTAAGAGGA GCTTGTCTGA AACAGTAACT CATAGGGCCA AAATCATGAA AATTGCTGCT 
AAGAAACGAA ATAGTGTTCA TGTTACTTTT AGACCATCTA CTGAGTCTGT CCAGTTTTAT 
AACCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TTCGGAAGCT CAGTGGCTTT 
AGCAGTAGTA GCAATAGCAG TACCAACAAC ACACAGACCG GCTCAACCAG TGGTACAGAC 
CTTGTAAAAC CTGGTGTGTA CAGACCTCTA GATATGATTG GTGCATCATC AGTTAGCAAA 
ACAGTGAAGG AATCCCCAGA GATTCCTACC ACATCACAGA AAGAAGGAAT TGCAAGTAAC 
CAGTTAGGGA GTCGCAGTAC TCTTAGGTCA TCAAGTCACG AGGCAGGACT ACAACAGGGC 
TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT 
TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT 
GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTAAATAAAG AGGACACGAT ACCCAAGCTG 
CCGCCTCTTA ATAGTGATAC TGGTGGCAGC AGTGCTAATG TTCCTGACCT GATGGATGAG 
TTCATAGCAG AGCGGCTTCG AAGCGGGAAC GCCTCGACTA TGGCAAGAAG GGGAAGTAGT 
CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG 
CGTCCTGTTG ATGACCCAGG TGTACCCTCA GAGTGGACAT CTCCTGCCAG TGCAGGCAGC 
AGTGACCTTA TGAGCTCGGA TAGTCATTCG GATTCTTTCA GTGCTTTCCA GTGTGATGGG 
CGAAAGTTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCGTCCTC TGCTGATGTG 
GATTCAGGTT CTGGCCATCA TCAGAGCACT GAGGAGCAGG AAGTAGCTAG CCTAACAACA 
CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC TGAAGTTGCA 
ACAGTTACTG GTTCAGAAAG TGCTTCCCCA GTTCACTCAG CTTTGGGATC CAGATCACAG 
ACACCTTCCC CATCTACATT AAATATAGAT CACATGGAGC AGAAGGATCT GCAGCTCGAT 
GAGAAGCTCC ACCACTCTGT TCTGCAGACG CCAGATGATC TAGAAATCAG TGAATTTCCA 
TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT 
ACCGTTATGT GGCGAAGAAT GTTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT 
GAAATACATG CTCAAGTGTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC 
AGAGATAATC TTGGCATTTC AGCAGATAAC CTGACTTCAC CCTCTCCACC TGTTTTGATT 
CCTCCACTAA GAATCCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT 
AAGCAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT 
GTATCTCCAA ATAGAGATTT CTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT 
CATATTGATC AGGACATTGT CAATACAATC ATCAAACACT GCTCACCTCA GTTTCTTTCA 
CTTGGTTTGC CTGGTGCCAC AATGCTCATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG 
GCTTCTTCAG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTG 
GTTTGCTTTC CCAACCTGTA CTGTGAACTG CCTGCTCTCC ATCCCAGCAT CCCTGAGATT 
GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCAGCA 
AGAGATGAAC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGCTTAGG TATTTGGATT 
TGTGAAGAAT TAGTTCATGA ATCTCATCAT CCTCAAATTA AAGAAGCTCT GAATGTAATT 
TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCATG TAGCTTGTAA CATGCTTCAC 
ATGCTGGTTC ACTATGTACC TAGACTTCAG ATTTACCAGC CGGATTCACC CTTGAAAATT 
ATTCAGATCT TAATAGCTAC TGTCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT 
GAAATGGACA AGAGGCTGGT TGTATCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC 
TTACCTTTAA AGACATTACT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAAACA 
GAAAAATCTG TCCTCAACTG CATTTATAAG GTATTACATG GGTGTGTATA TGGAGCGCAA 
TGCTTTAGCA ATCCGAAGTA TTTTCCAATA AGCCTCTCTG ATTTGGCATC TGTAGATTAT 
GACCCTTTTA TGCATTTGGA AAATCTGAAA GAGCCAGAGC CTCTACACTC TCCTGATTCA 
GAACGATCTT CTAAACTCCA GCCCGTAACA GAAGTGAAAA CTCAGATGCA ACAAGGATTG 
ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA 
ATGAGTGGTG GTCCTGCTAT GCTGACAAGT CAGGTCTGTG AAAATCATGA CAATCATTAC 
AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAATATTCA GTTCTTTGTG 
CTGAACAATA CAACTTTGGT GTCTTGTATC CAGATAAGAG CAGAAGAAAG TATTCCTGGA 
GGAGGGTTAG CAGCTGGCCT TGTGTCAGCC AATTCAAATG TTAGAATCAT AGTGCGTGAT 
CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GGCCACCTAT TGTGAATAGT 
TTGTCTGAAC CTACGTCTTT CATACTTTCA ATGTCTCACC AAGAGAAGCC AGAAGAGCCT 
CCTACATCTA ATGAATGCTT AGAAGACGTA ACAGTAAAAG ATGGACTTTC CCTACAACTT 
AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACCATAA GGGAGGAGGA AGATGCTCTT 
GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAAT GTTTACAGAG AACTGGAATC 
TCCCTCAATG TTCCTGCTCC CCAACCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTC 
ATTAATGCTA TCCTTAAGCA ATACACAGAA GAAAAAGAGT TTGTGGAGAA GCACTTTAAT 
GACTTAAACA TGAAAGCCTC AGAACAAGAT GAACCAACCC CTCAGAGGCC TCAGTCAGCA 
TTTTATTACT GCAGACTGCT TCTTAGTATC TTGGGAATGA ATTCCTGGGA CAAACGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTGCTTCGAG AACTTAGGAA CTTGGATTCG 
AGACAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCGGAAGG ACAAGAAGAT 
AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGATAAATCT TACAAATCAT TGTGGTTTCA TGGGAGGATT ACAGAAGAAC 
AAAAGCACTG GACTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAAGT AATATTTCAT 
GTATCAACAA GAATGCCTTC TGATTCTGAT GATTCTCTGA CCAAAAAATT GAGACACCTA 
GGAAATGATG AAGTACACAT TGTTTGGTCA GAACATACTA GAGACTACAG AAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTCATT GTAATATATC CAATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT 
GCTCTGAAAT CCCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTACCTG 
CAAACAATTG TTCAGCACCA TTTAGAACCA ACAACATTTG AAGACTTTGC AGCACAGGTT 
TTCTCTCCAG CACCCTACCA TCACTTACCT TCTGATGCAG TTGGATCCTA CCCAGAGAGT 
CTGCCCAGTG ACACTCCCAC AGCAGCCCAG GTAGACGGGG CTGACCTGGC CTCTCCAATG 
TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGGTCCTC TGGCTCAGCC 
AATAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012976293.1
CDS	130..7101
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQDNCSKEQLWMFSCLIPGFSAPQSDYGPRTLDNLINPPLNLQETQVTIEEVTPLVPPQSGDKGQEDLTSYFLEALLKYIVIQENGTNISEHVRNSSWTKNGSYQDAFHASEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRSTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDLVEPFTVERAKGAVPVVDTSSRHAPSLQTSTEASSVTRSTESCSTDTRSRESSLEVGDSIYDHLCHLIGPVELADAAFEQIQYIDLEGDDDLLSSLKEYFKENQENHSNNELGKDTASQKVIIAVTRDEIPSLDKLDYIGQEIKSENLMSYVGTPENTPFQKQPSSAVFTSTVAPKQLVSFSTTQAAEKPMQLNLDEQSSDPSSDSPCDKEKKKHLYRQTATEVDACVDMTLGEKLKSIQVNEKITPYIMHAKKTALKAPVNRRMPHVANTSKLSPTKRSLSETVTHRAKIMKIAAKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSSSSNSSTNNTQTGSTSGTDLVKPGVYRPLDMIGASSVSKTVKESPEIPTTSQKEGIASNQLGSRSTLRSSSHEAGLQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDTIPKLPPLNSDTGGSSANVPDLMDEFIAERLRSGNASTMARRGSSPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHSDSFSAFQCDGRKFDNFGFGTDIGIPSSADVDSGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFLSLGLPGATMLIMDFIIAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPSIPEIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATVTHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKTEKSVLNCIYKVLHGCVYGAQCFSNPKYFPISLSDLASVDYDPFMHLENLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVNSLSEPTSFILSMSHQEKPEEPPTSNECLEDVTVKDGLSLQLRRFRETVPTWSTIREEEDALDELLQYLGTTSPECLQRTGISLNVPAPQPVCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQRPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGWEINLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPESLPSDTPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_013120839.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus Ral GTPase activating protein, alpha subunit 1 (catalytic) (Ralgapa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013120839.1

ORF Insert Sequence:

ATGTTTTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT 
ATTGACCTTA AGCAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGCC ACAAGTCTCA GAGAGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AACTGCTTCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCGCTTCAG 
GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG ACTATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TCCCCCCGCA GTCAGGAGAT 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGAAAATG GGACCAATAT TTCTGAGCAT GTTCGAAATT CTAGTTGGAC CAAAAATGGT 
TCCTACCAAG ATGCTTTTCA TGCCTCTGAA GAAGCCACAG AACAAAATAT TCAAGCTGGT 
ACGCAGGCAG TTTTGCAGGT GTTCATTATA AACTCATCAA ACATATTTCT TCTTGAACCT 
GCCAATGAAA TAAAAAATCT TTTGGATGAG CACACAGATA TGTGTAAACG TATTCTTAAC 
ATTTATCGAT ACATGGTTGT GCAAGTATCA ATGGACAAAA AGACCTGGGA ACAGATGCTG 
CTCGTGCTGC TCAGAGTCAC AGAGTCTGTA CTGAAGATGT CATCACAAGC TTTTCTACAG 
TTCCAGGGGA AAAAAAACAT GACCTTGGCA GGTCGACTTG CAGGACCACT TTTCCAGACT 
CTCATTGTTG CCTGGATCAA AGCAAATCTA AATGTGTACA TCTCTCGAGA ACTTTGGGAT 
GACTTACTTT CAGTATTGTC ATCGTTGACC TATTGGGAAG AATTGGCCAC TGAGTGGTCT 
CTGACCATGG AGACGCTAAC TAAAGTTTTA GCTAGGAATT TATATAGTTT GGATCTCAGT 
GATTTGCCGT TGGATAAGCT GAGTGAACAG AAACAAAAAA AGCACAAAGG GAAAGGAGTT 
GGACATGAAT TTCAGAAAGT TTCAGTTGAC AAGTCATTTT CTAGAGGATG GAGTCGTGAT 
CAGCCTGGCC AGGCACCCAT GAGACAAAGA AGTGCAACAA CTACTGGGTC CCCAGGAACT 
GAAAAGGCAA GGAGTATAGT ACGACAAAAA ACTGTTGCCA TGAGAAGCCG ATCCATTGGT 
GAATGTGCTC TGCCATCGGC CTATATTCGC AGTGCTAAAA GTGCTCCTGT TCTGATCCAT 
ACTTCCAAAC CCTTCTTGCC TGATATTGTT CTCACTCCCC TTTCTGATGA GCTTTCAGAT 
ATTGATGATG CTCAAATCCT TCCTCGCTCA ACCAGAGTCA GACATTTTTC ACAGAGCGAA 
GACACTGGAA ATGAAGTGTT TGGAGCTCTG AGTGAGGAAC AGCCATTGCC TCGAAGCAGC 
AGCACTTCTG ACCTCGTGGA GCCGTTCACT GTTGAGCGAG CCAAAGGTGC AGTTCCTGTC 
GTTGACACTT CATCCCGTCA TGCTCCGAGC TTGCAGACTT CCACAGAGGC GTCTTCAGTA 
ACTAGATCCA CTGAGAGCTG CAGCACTGAT ACTCGTAGTA GAGAGTCTTC TCTGGAAGTT 
GGTGATAGCA TATATGACCA TCTTTGTCAT TTAATAGGTC CAGTAGAACT TGCAGATGCA 
GCTTTCGAAC AAATCCAGTA CATTGACCTT GAAGGAGATG ATGATCTTCT TTCTTCCCTG 
AAAGAATACT TTAAGGAAAA CCAGGAAAAT CATAGTAACA ATGAGCTAGG TAAAGATACA 
GCTTCTCAGA AGGTGATTAT TGCAGTAACT AGAGATGAAA TACCATCTTT AGATAAACTA 
GACTACATAG GCCAGGAAAT AAAATCAGAA AATCTCATGT CTTATGTTGG GACTCCTGAA 
AATACACCAT TTCAAAAACA ACCAAGCTCA GCTGTCTTCA CGAGTACTGT TGCACCTAAG 
CAGTTAGTTA GTTTTTCAAC AACACAAGCT GCTGAAAAAC CTATGCAACT AAATCTTGAT 
GAACAGTCAT CGGATCCTAG TTCAGATAGC CCTTGTGATA AAGAAAAAAA GAAACATCTG 
TATAGACAAA CAGCCACAGA AGTAGATGCC TGTGTAGATA TGACACTAGG TGAAAAGCTT 
AAGAGTATAC AAGTTAATGA AAAAATCACC CCATATATAA TGCATGCCAA GAAAACTGCA 
CTAAAAGCAC CAGTGAACCG CAGAATGCCT CATGTTGCAA ACACTAGCAA GCTTTCTCCA 
ACTAAGAGGA GCTTGTCTGA AACAGTAACT CATAGGGCCA AAATCATGAA AATTGCTGCT 
AAGAAACGAA ATAGTGTTCA TGTTACTTTT AGACCATCTA CTGAGTCTGT CCAGTTTTAT 
AACCCTCTGG AAAACAAAGA GGCTCCATGG AAGATGAGAC TTCGGAAGCT CAGTGGCTTT 
AGCAGTAGTA GCAATAGCAG TACCAACAAC ACACAGACCG GCTCAACCAG TGGTACAGAC 
CTTGTAAAAC CTGGTGTGTA CAGACCTCTA GATATGATTG GTGCATCATC AGTTAGCAAA 
ACAGTGAAGG AATCCCCAGA GATTCCTACC ACATCACAGA AAGAAGGAAT TGCAAGTAAC 
CAGTTAGGGA GTCGCAGTAC TCTTAGGTCA TCAAGTCACG AGGCAGGACT ACAACAGGGC 
TCCCTGGGTG GCGTTTATAA AACTGTTGTA CATGCTCTTT CGAAGCCGAA GGCAAATGTT 
TCCCCACAGA GGCAGAACAG AATGCCACCA GAGGCTCCAC TGAGGGATCT GTACAGTCAT 
GTAATGGGCT ATTTTGGAAG GAAAGCTGCA GTAAATAAAG AGGACACGAT ACCCAAGCTG 
CCGCCTCTTA ATAGTGATAC TGGTGGCAGC AGTGCTAATG TTCCTGACCT GATGGATGAG 
TTCATAGCAG AGCGGCTTCG AAGCGGGAAC GCCTCGACTA TGGCAAGAAG GGGAAGTAGT 
CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG 
CGTCCTGTTG ATGACCCAGG TGTACCCTCA GAGTGGACAT CTCCTGCCAG TGCAGGCAGC 
AGTGACCTTA TGAGCTCGGA TAGTCATTCG GATTCTTTCA GTGCTTTCCA GTGTGATGGG 
CGAAAGTTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCGTCCTC TGCTGATGTG 
GATTCAGGTT CTGGCCATCA TCAGAGCACT GAGGAGCAGG AAGTAGCTAG CCTAACAACA 
CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC TGAAGTTGCA 
ACAGTTACTG GTTCAGAAAG TGCTTCCCCA GTTCACTCAG CTTTGGGATC CAGATCACAG 
ACACCTTCCC CATCTACATT AAATATAGAT CACATGGAGC AGAAGGATCT GCAGCTCGAT 
GAGAAGCTCC ACCACTCTGT TCTGCAGACG CCAGATGATC TAGAAATCAG TGAATTTCCA 
TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT 
ACCGTTATGT GGCGAAGAAT GTTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT 
GAAATACATG CTCAAGTGTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC 
AGAGATAATC TTGGCATTTC AGCAGATAAC CTGACTTCAC CCTCTCCACC TGTTTTGATT 
CCTCCACTAA GAATCCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT 
AAGCAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT 
GTATCTCCAA ATAGAGATTT CTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT 
CATATTGATC AGGACATTGT CAATACAATC ATCAAACACT GCTCACCTCA GTTTCTTTCA 
CTTGGTTTGC CTGGTGCCAC AATGCTCATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG 
GCTTCTTCAG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTG 
GTTTGCTTTC CCAACCTGTA CTGTGAACTG CCTGCTCTCC ATCCCAGCAT CCCTGAGATT 
GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCAGCA 
AGAGATGAAC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGCTTAGG TATTTGGATT 
TGTGAAGAAT TAGTTCATGA ATCTCATCAT CCTCAAATTA AAGAAGCTCT GAATGTAATT 
TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCATG TAGCTTGTAA CATGCTTCAC 
ATGCTGGTTC ACTATGTACC TAGACTTCAG ATTTACCAGC CGGATTCACC CTTGAAAATT 
ATTCAGATCT TAATAGCTAC TGTCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT 
GAAATGGACA AGAGGCTGGT TGTATCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC 
TTACCTTTAA AGACATTACT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAAACA 
GAAAAATCTG TCCTCAACTG CATTTATAAG GTATTACATG GGTGTGTATA TGGAGCGCAA 
TGCTTTAGCA ATCCGAAGTA TTTTCCAATA AGCCTCTCTG ATTTGGCATC TGTAGATTAT 
GACCCTTTTA TGCATTTGGA AAATCTGAAA GAGCCAGAGC CTCTACACTC TCCTGATTCA 
GAACGATCTT CTAAACTCCA GCCCGTAACA GAAGTGAAAA CTCAGATGCA ACAAGGATTG 
ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA 
ATGAGTGGTG GTCCTGCTAT GCTGACAAGT CAGGTCTGTG AAAATCATGA CAATCATTAC 
AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAATATTCA GTTCTTTGTG 
CTGAACAATA CAACTTTGGT GTCTTGTATC CAGATAAGAG CAGAAGAAAG TATTCCTGGA 
GGAGGGTTAG CAGCTGGCCT TGTGTCAGCC AATTCAAATG TTAGAATCAT AGTGCGTGAT 
CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GGCCACCTAT TGTGAATAGT 
TTGTCTGAAC CTACGTCTTT CATACTTTCA ATGTCTCACC AAGAGAAGCC AGAAGAGCCT 
CCTACATCTA ATGAATGCTT AGAAGACGTA ACAGTAAAAG ATGGACTTTC CCTACAACTT 
AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACCATAA GGGAGGAGGA AGATGCTCTT 
GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAAT GTTTACAGAG AACTGGAATC 
TCCCTCAATG TTCCTGCTCC CCAACCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTC 
ATTAATGCTA TCCTTAAGCA ATACACAGAA GAAAAAGAGT TTGTGGAGAA GCACTTTAAT 
GACTTAAACA TGAAAGCCTC AGAACAAGAT GAACCAACCC CTCAGAGGCC TCAGTCAGCA 
TTTTATTACT GCAGACTGCT TCTTAGTATC TTGGGAATGA ATTCCTGGGA CAAACGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTGCTTCGAG AACTTAGGAA CTTGGATTCG 
AGACAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCGGAAGG ACAAGAAGAT 
AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGATAAATCT TACAAATCAT TGTGGTTTCA TGGGAGGATT ACAGAAGAAC 
AAAAGCACTG GACTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAAGT AATATTTCAT 
GTATCAACAA GAATGCCTTC TGATTCTGAT GATTCTCTGA CCAAAAAATT GAGACACCTA 
GGAAATGATG AAGTACACAT TGTTTGGTCA GAACATACTA GAGACTACAG AAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTCATT GTAATATATC CAATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT 
GCTCTGAAAT CCCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTACCTG 
CAAACAATTG TTCAGCACCA TTTAGAACCA ACAACATTTG AAGACTTTGC AGCACAGGTT 
TTCTCTCCAG CACCCTACCA TCACTTACCT TCTGATGCAG TTGGATCCTA CCCAGAGAGT 
CTGCCCAGTG ACACTCCCAC AGCAGCCCAG GTAGACGGGG CTGACCTGGC CTCTCCAATG 
TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGGTCCTC TGGCTCAGCC 
AATAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ralgapa1 ( XM_021233220.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021088879.1 Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X1, mRNA.

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CloneID	OMe252639
Clone ID Related Accession (Same CDS sequence)	XM_021233220.1
Accession Version	XM_021233220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	ral GTPase-activating protein subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801703.1) and transcript sequence (GDQJ01000294.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 786 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTTTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT 
ATTGACCTTA AGCAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGCC ACAAGTCTCA GAGAGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AACTGCTTCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCGCTTCAG 
GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG ACTATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TCCCCCCGCA GTCAGGAGAT 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATCTG CAAGGAAAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCTCA TTATGTGATG 
ATAAAGAAGG ATGCTGAAAC CAATGAAGCA ATATACTGTA CAAAAGAGCC TTTCATCCAA 
GCTCGGGTTA TTGTCATTCG ATGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA 
GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG TTGATAAAGC AGACAAAACA 
ACAGAGCCAG AACAATCTCA TTCTAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGCATCGA TGAAGAACAT CTCACAGACA TTGAAATTGT TCGCAGAGTG 
TTTTCATCTA AAAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGCCA GGCATTTTTA 
TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTGTATCA AGAATGGATT 
CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAAG AACGTGTGCT TAGTTCTTCA 
GATATACCTT GCCTTGAAAA TGTCACAGAC CATGATATTT CAATAGAAGA TGGAGAAAAA 
CGAGAAGAGG AAAATGGGAC CAATATTTCT GAGCATGTTC GAAATTCTAG TTGGACCAAA 
AATGGTTCCT ACCAAGATGC TTTTCATGCC TCTGAAGAAG CCACAGAACA AAATATTCAA 
GCTGGTACGC AGGCAGTTTT GCAGGTGTTC ATTATAAACT CATCAAACAT ATTTCTTCTT 
GAACCTGCCA ATGAAATAAA AAATCTTTTG GATGAGCACA CAGATATGTG TAAACGTATT 
CTTAACATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG 
ATGCTGCTCG TGCTGCTCAG AGTCACAGAG TCTGTACTGA AGATGTCATC ACAAGCTTTT 
CTACAGTTCC AGGGGAAAAA AAACATGACC TTGGCAGGTC GACTTGCAGG ACCACTTTTC 
CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TGTACATCTC TCGAGAACTT 
TGGGATGACT TACTTTCAGT ATTGTCATCG TTGACCTATT GGGAAGAATT GGCCACTGAG 
TGGTCTCTGA CCATGGAGAC GCTAACTAAA GTTTTAGCTA GGAATTTATA TAGTTTGGAT 
CTCAGTGATT TGCCGTTGGA TAAGCTGAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA 
GGAGTTGGAC ATGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT 
CGTGATCAGC CTGGCCAGGC ACCCATGAGA CAAAGAAGTG CAACAACTAC TGGGTCCCCA 
GGAACTGAAA AGGCAAGGAG TATAGTACGA CAAAAAACTG TTGCCATGAG AAGCCGATCC 
ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG 
ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT 
TCAGATATTG ATGATGCTCA AATCCTTCCT CGCTCAACCA GAGTCAGACA TTTTTCACAG 
AGCGAAGACA CTGGAAATGA AGTGTTTGGA GCTCTGAGTG AGGAACAGCC ATTGCCTCGA 
AGCAGCAGCA CTTCTGACCT CGTGGAGCCG TTCACTGTTG AGCGAGCCAA AGGTCCAGTA 
GAACTTGCAG ATGCAGCTTT CGAACAAATC CAGTACATTG ACCTTGAAGG AGATGATGAT 
CTTCTTTCTT CCCTGAAAGA ATACTTTAAG GAAAACCAGG AAAATCATAG TAACAATGAG 
CTAGGTAAAG ATACAGCTTC TCAGAAGGTG ATTATTGCAG TAACTAGAGA TGAAATACCA 
TCTTTAGATA AACTAGACTA CATAGGCCAG GAAATAAAAT CAGAAAATCT CATGTCTTAT 
GTTGGGACTC CTGAAAATAC ACCATTTCAA AAACAACCAA GCTCAGCTGT CTTCACGAGT 
ACTGTTGCAC CTAAGCAGTT AGTTAGTTTT TCAACAACAC AAGCTGCTGA AAAACCTATG 
CAACTAAATC TTGATGAACA GTCATCGGAT CCTAGTTCAG ATAGCCCTTG TGATAAAGAA 
AAAAAGAAAC ATCTGTATAG ACAAACAGCC ACAGAAGTAG ATGCCTGTGT AGATATGACA 
CTAGGTGAAA AGCTTAAGAG TATACAAGTT AATGAAAAAA TCACCCCATA TATAATGCAT 
GCCAAGAAAA CTGCACTAAA AGCACCAGTG AACCGCAGAA TGCCTCATGT TGCAAACACT 
AGCAAGCTTT CTCCAACTAA GAGGAGCTTG TCTGAAACAG TAACTCATAG GGCCAAAATC 
ATGAAAATTG CTGCTAAGAA ACGAAATAGT GTTCATGTTA CTTTTAGACC ATCTACTGAG 
TCTGTCCAGT TTTATAACCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTTCGG 
AAGCTCAGTG GCTTTAGCAG TAGTAGCAAT AGCAGTACCA ACAACACACA GACCGGCTCA 
ACCAGTGGTA CAGACCTTGT AAAACCTGGT GTGTACAGAC CTCTAGATAT GATTGGTGCA 
TCATCAGTTA GCAAAACAGT GAAGGAATCC CCAGAGATTC CTACCACATC ACAGAAAGAA 
GGAATTGCAA GTAACCAGTT AGGGAGTCGC AGTACTCTTA GGTCATCAAG TCACGAGGCA 
GGACTACAAC AGGGCTCCCT GGGTGGCGTT TATAAAACTG TTGTACATGC TCTTTCGAAG 
CCGAAGGCAA ATGTTTCCCC ACAGAGGCAG AACAGAATGC CACCAGAGGC TCCACTGAGG 
GATCTGTACA GTCATGTAAT GGGCTATTTT GGAAGGAAAG CTGCAGTAAA TAAAGAGGAC 
ACGATACCCA AGCTGCCGCC TCTTAATAGT GATACTGGTG GCAGCAGTGC TAATGTTCCT 
GACCTGATGG ATGAGTTCAT AGCAGAGCGG CTTCGAAGCG GGAACGCCTC GACTATGGCA 
AGAAGGGGAA GTAGTCCAGG CAGCCTTGAA ATCCCGAAAG ACCTCCCTGA TATTCTAAAC 
AAGCAGAACC AGATGCGTCC TGTTGATGAC CCAGGTGTAC CCTCAGAGTG GACATCTCCT 
GCCAGTGCAG GCAGCAGTGA CCTTATGAGC TCGGATAGTC ATTCGGATTC TTTCAGTGCT 
TTCCAGTGTG ATGGGCGAAA GTTTGACAAC TTTGGCTTCG GGACTGACAT TGGGATCCCG 
TCCTCTGCTG ATGTGGATTC AGGTTCTGGC CATCATCAGA GCACTGAGGA GCAGGAAGTA 
GCTAGCCTAA CAACACTTCA TTTAGATTCA GAAACAAGCA GCCTTAATCA GCAAGCCTTC 
TCTGCTGAAG TTGCAACAGT TACTGGTTCA GAAAGTGCTT CCCCAGTTCA CTCAGCTTTG 
GGATCCAGAT CACAGACACC TTCCCCATCT ACATTAAATA TAGATCACAT GGAGCAGAAG 
GATCTGCAGC TCGATGAGAA GCTCCACCAC TCTGTTCTGC AGACGCCAGA TGATCTAGAA 
ATCAGTGAAT TTCCATCTGA ATGTTGTAGC GTGATGGCAG GAGGTACTCT CACTGGGTGG 
CATGCTGATG TTGCTACCGT TATGTGGCGA AGAATGTTGG GCATTTTGGG AGATGTCAAT 
GCTATTATGG ATCCTGAAAT ACATGCTCAA GTGTTTGATT ACCTCTGTGA ACTTTGGCAG 
AATCTAGCCA AGATCAGAGA TAATCTTGGC ATTTCAGCAG ATAACCTGAC TTCACCCTCT 
CCACCTGTTT TGATTCCTCC ACTAAGAATC CTTACACCTT GGCTTTTTAA GGCAACCATG 
TTGACTGATA AGTATAAGCA AGGTAAATTA CATGCATATA AACTTATTTG TAATACAATG 
AAGAGAAGAC AAGATGTATC TCCAAATAGA GATTTCTTAA CACATTTCTA CAATATAATG 
CATTGTGGAT TGCTTCATAT TGATCAGGAC ATTGTCAATA CAATCATCAA ACACTGCTCA 
CCTCAGTTTC TTTCACTTGG TTTGCCTGGT GCCACAATGC TCATTATGGA TTTTATTATA 
GCAGCTGGGA GAGTGGCTTC TTCAGCTTTT CTTAATGCAC CAAGAGTGGA AGCACAAGTT 
CTTCTGGGAT CTTTGGTTTG CTTTCCCAAC CTGTACTGTG AACTGCCTGC TCTCCATCCC 
AGCATCCCTG AGATTGCTGT GTCTCAGTTT ACAGATGTTA AGGAACTTAT AATTAAAACT 
GTATTAAGCT CAGCAAGAGA TGAACCCTCT GGTCCTGCAC GATGTGTAGC ACTTTGCAGC 
TTAGGTATTT GGATTTGTGA AGAATTAGTT CATGAATCTC ATCATCCTCA AATTAAAGAA 
GCTCTGAATG TAATTTGTGT TTCATTAAAG TTTACTAATA AAACAGTTGC CCATGTAGCT 
TGTAACATGC TTCACATGCT GGTTCACTAT GTACCTAGAC TTCAGATTTA CCAGCCGGAT 
TCACCCTTGA AAATTATTCA GATCTTAATA GCTACTGTCA CCCACCTTTT GCCAAGTACA 
GAAGCTTCAT CATATGAAAT GGACAAGAGG CTGGTTGTAT CATTACTTCT TTGCCTTCTG 
GACTGGATCA TGGCCTTACC TTTAAAGACA TTACTCCAAC CAGTCCATGC TACAGGAGCA 
GAAAATGATA AAACAGAAAA ATCTGTCCTC AACTGCATTT ATAAGGTATT ACATGGGTGT 
GTATATGGAG CGCAATGCTT TAGCAATCCG AAGTATTTTC CAATAAGCCT CTCTGATTTG 
GCATCTGTAG ATTATGACCC TTTTATGCAT TTGGAAAATC TGAAAGAGCC AGAGCCTCTA 
CACTCTCCTG ATTCAGAACG ATCTTCTAAA CTCCAGCCCG TAACAGAAGT GAAAACTCAG 
ATGCAACAAG GATTGATCTC TATAGCAGCC CGTACTGTTA TTACCCATCT GGTGAATCAC 
TTGGGCCATT ATCCAATGAG TGGTGGTCCT GCTATGCTGA CAAGTCAGGT CTGTGAAAAT 
CATGACAATC ATTACAGTGA AAGTACTGAA CTTTCTCCTG AGCTCTTTGA AAGTCCAAAT 
ATTCAGTTCT TTGTGCTGAA CAATACAACT TTGGTGTCTT GTATCCAGAT AAGAGCAGAA 
GAAAGTATTC CTGGAGGAGG GTTAGCAGCT GGCCTTGTGT CAGCCAATTC AAATGTTAGA 
ATCATAGTGC GTGATCTCTC TGGAAAGTAC TCATGGGATT CTGCCATACT GTATGGGCCA 
CCTATTGTGA ATAGTTTGTC TGAACCTACG TCTTTCATAC TTTCAATGTC TCACCAAGAG 
AAGCCAGAAG AGCCTCCTAC ATCTAATGAA TGCTTAGAAG ACGTAACAGT AAAAGATGGA 
CTTTCCCTAC AACTTAGAAG ATTTAGAGAA ACTGTACCAA CTTGGTCTAC CATAAGGGAG 
GAGGAAGATG CTCTTGACGA GCTCCTGCAG TATTTAGGCA CTACAAGTCC TGAATGTTTA 
CAGAGAACTG GAATCTCCCT CAATGTTCCT GCTCCCCAAC CTGTGTGCAT TTCTGAAAAA 
CAGGAGAATG ATGTCATTAA TGCTATCCTT AAGCAATACA CAGAAGAAAA AGAGTTTGTG 
GAGAAGCACT TTAATGACTT AAACATGAAA GCCTCAGAAC AAGATGAACC AACCCCTCAG 
AGGCCTCAGT CAGCATTTTA TTACTGCAGA CTGCTTCTTA GTATCTTGGG AATGAATTCC 
TGGGACAAAC GGAGGAGCTT TCATCTCCTG AAGAAAAATG AAAAGCTGCT TCGAGAACTT 
AGGAACTTGG ATTCGAGACA ATGCCGAGAG ACACACAAGA TTGCAGTATT TTACGTTGCG 
GAAGGACAAG AAGATAAATA CTCCATTCTC ACCAATATAG GAGGAAGCCA AGCATATGAA 
GATTTTGTAG CTGGTCTTGG TTGGGAGATA AATCTTACAA ATCATTGTGG TTTCATGGGA 
GGATTACAGA AGAACAAAAG CACTGGACTG ACCACTCCAT ATTTTGCTAC CTCTACAGTA 
GAAGTAATAT TTCATGTATC AACAAGAATG CCTTCTGATT CTGATGATTC TCTGACCAAA 
AAATTGAGAC ACCTAGGAAA TGATGAAGTA CACATTGTTT GGTCAGAACA TACTAGAGAC 
TACAGAAGAG GAATTATTCC TACAGAATTT GGTGATGTCC TCATTGTAAT ATATCCAATG 
AAAAACCACA TGTTCAGTAT CCAGATCATG AAAAAACCAG AGGTTCCCTT CTTTGGTCCA 
CTTTTTGATG GTGCTATTGT GAATGGAAAA GTTCTGCCCA TTATGGTTCG ATCCACAGCA 
ATAAATGCTA GCCGTGCTCT GAAATCCCTG ATTCCATTGT ATCAAAACTT CTATGAGGAG 
AGAGCACGGT ACCTGCAAAC AATTGTTCAG CACCATTTAG AACCAACAAC ATTTGAAGAC 
TTTGCAGCAC AGGTTTTCTC TCCAGCACCC TACCATCACT TACCTTCTGA TGCAGTTGGA 
TCCTACCCAG AGAGTCTGCC CAGTGACACT CCCACAGCAG CCCAGGTAGA CGGGGCTGAC 
CTGGCCTCTC CAATGTCTCC TCGAACTAGC AAAAGCCGCA TGTCCATGAA GCTGCGTCGG 
TCCTCTGGCT CAGCCAATAA ATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021088879.1
CDS	105..7691
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQDNCSKEQLWMFSCLIPGFSAPQSDYGPRTLDNLINPPLNLQETQVTIEEVTPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREENKNDNVDKADKTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEERVLSSSDIPCLENVTDHDISIEDGEKREEENGTNISEHVRNSSWTKNGSYQDAFHASEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRSTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDLVEPFTVERAKGPVELADAAFEQIQYIDLEGDDDLLSSLKEYFKENQENHSNNELGKDTASQKVIIAVTRDEIPSLDKLDYIGQEIKSENLMSYVGTPENTPFQKQPSSAVFTSTVAPKQLVSFSTTQAAEKPMQLNLDEQSSDPSSDSPCDKEKKKHLYRQTATEVDACVDMTLGEKLKSIQVNEKITPYIMHAKKTALKAPVNRRMPHVANTSKLSPTKRSLSETVTHRAKIMKIAAKKRNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLSGFSSSSNSSTNNTQTGSTSGTDLVKPGVYRPLDMIGASSVSKTVKESPEIPTTSQKEGIASNQLGSRSTLRSSSHEAGLQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNKEDTIPKLPPLNSDTGGSSANVPDLMDEFIAERLRSGNASTMARRGSSPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHSDSFSAFQCDGRKFDNFGFGTDIGIPSSADVDSGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFLSLGLPGATMLIMDFIIAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPSIPEIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATVTHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKTEKSVLNCIYKVLHGCVYGAQCFSNPKYFPISLSDLASVDYDPFMHLENLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVNSLSEPTSFILSMSHQEKPEEPPTSNECLEDVTVKDGLSLQLRRFRETVPTWSTIREEEDALDELLQYLGTTSPECLQRTGISLNVPAPQPVCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQRPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGWEINLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPESLPSDTPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_021233220.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021233220.1

ORF Insert Sequence:

ATGTTTTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT 
ATTGACCTTA AGCAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGCC ACAAGTCTCA GAGAGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AACTGCTTCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCGCTTCAG 
GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG ACTATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TCCCCCCGCA GTCAGGAGAT 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATCTG CAAGGAAAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCTCA TTATGTGATG 
ATAAAGAAGG ATGCTGAAAC CAATGAAGCA ATATACTGTA CAAAAGAGCC TTTCATCCAA 
GCTCGGGTTA TTGTCATTCG ATGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA 
GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG TTGATAAAGC AGACAAAACA 
ACAGAGCCAG AACAATCTCA TTCTAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGCATCGA TGAAGAACAT CTCACAGACA TTGAAATTGT TCGCAGAGTG 
TTTTCATCTA AAAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGCCA GGCATTTTTA 
TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTGTATCA AGAATGGATT 
CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAAG AACGTGTGCT TAGTTCTTCA 
GATATACCTT GCCTTGAAAA TGTCACAGAC CATGATATTT CAATAGAAGA TGGAGAAAAA 
CGAGAAGAGG AAAATGGGAC CAATATTTCT GAGCATGTTC GAAATTCTAG TTGGACCAAA 
AATGGTTCCT ACCAAGATGC TTTTCATGCC TCTGAAGAAG CCACAGAACA AAATATTCAA 
GCTGGTACGC AGGCAGTTTT GCAGGTGTTC ATTATAAACT CATCAAACAT ATTTCTTCTT 
GAACCTGCCA ATGAAATAAA AAATCTTTTG GATGAGCACA CAGATATGTG TAAACGTATT 
CTTAACATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG 
ATGCTGCTCG TGCTGCTCAG AGTCACAGAG TCTGTACTGA AGATGTCATC ACAAGCTTTT 
CTACAGTTCC AGGGGAAAAA AAACATGACC TTGGCAGGTC GACTTGCAGG ACCACTTTTC 
CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TGTACATCTC TCGAGAACTT 
TGGGATGACT TACTTTCAGT ATTGTCATCG TTGACCTATT GGGAAGAATT GGCCACTGAG 
TGGTCTCTGA CCATGGAGAC GCTAACTAAA GTTTTAGCTA GGAATTTATA TAGTTTGGAT 
CTCAGTGATT TGCCGTTGGA TAAGCTGAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA 
GGAGTTGGAC ATGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT 
CGTGATCAGC CTGGCCAGGC ACCCATGAGA CAAAGAAGTG CAACAACTAC TGGGTCCCCA 
GGAACTGAAA AGGCAAGGAG TATAGTACGA CAAAAAACTG TTGCCATGAG AAGCCGATCC 
ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG 
ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT 
TCAGATATTG ATGATGCTCA AATCCTTCCT CGCTCAACCA GAGTCAGACA TTTTTCACAG 
AGCGAAGACA CTGGAAATGA AGTGTTTGGA GCTCTGAGTG AGGAACAGCC ATTGCCTCGA 
AGCAGCAGCA CTTCTGACCT CGTGGAGCCG TTCACTGTTG AGCGAGCCAA AGGTCCAGTA 
GAACTTGCAG ATGCAGCTTT CGAACAAATC CAGTACATTG ACCTTGAAGG AGATGATGAT 
CTTCTTTCTT CCCTGAAAGA ATACTTTAAG GAAAACCAGG AAAATCATAG TAACAATGAG 
CTAGGTAAAG ATACAGCTTC TCAGAAGGTG ATTATTGCAG TAACTAGAGA TGAAATACCA 
TCTTTAGATA AACTAGACTA CATAGGCCAG GAAATAAAAT CAGAAAATCT CATGTCTTAT 
GTTGGGACTC CTGAAAATAC ACCATTTCAA AAACAACCAA GCTCAGCTGT CTTCACGAGT 
ACTGTTGCAC CTAAGCAGTT AGTTAGTTTT TCAACAACAC AAGCTGCTGA AAAACCTATG 
CAACTAAATC TTGATGAACA GTCATCGGAT CCTAGTTCAG ATAGCCCTTG TGATAAAGAA 
AAAAAGAAAC ATCTGTATAG ACAAACAGCC ACAGAAGTAG ATGCCTGTGT AGATATGACA 
CTAGGTGAAA AGCTTAAGAG TATACAAGTT AATGAAAAAA TCACCCCATA TATAATGCAT 
GCCAAGAAAA CTGCACTAAA AGCACCAGTG AACCGCAGAA TGCCTCATGT TGCAAACACT 
AGCAAGCTTT CTCCAACTAA GAGGAGCTTG TCTGAAACAG TAACTCATAG GGCCAAAATC 
ATGAAAATTG CTGCTAAGAA ACGAAATAGT GTTCATGTTA CTTTTAGACC ATCTACTGAG 
TCTGTCCAGT TTTATAACCC TCTGGAAAAC AAAGAGGCTC CATGGAAGAT GAGACTTCGG 
AAGCTCAGTG GCTTTAGCAG TAGTAGCAAT AGCAGTACCA ACAACACACA GACCGGCTCA 
ACCAGTGGTA CAGACCTTGT AAAACCTGGT GTGTACAGAC CTCTAGATAT GATTGGTGCA 
TCATCAGTTA GCAAAACAGT GAAGGAATCC CCAGAGATTC CTACCACATC ACAGAAAGAA 
GGAATTGCAA GTAACCAGTT AGGGAGTCGC AGTACTCTTA GGTCATCAAG TCACGAGGCA 
GGACTACAAC AGGGCTCCCT GGGTGGCGTT TATAAAACTG TTGTACATGC TCTTTCGAAG 
CCGAAGGCAA ATGTTTCCCC ACAGAGGCAG AACAGAATGC CACCAGAGGC TCCACTGAGG 
GATCTGTACA GTCATGTAAT GGGCTATTTT GGAAGGAAAG CTGCAGTAAA TAAAGAGGAC 
ACGATACCCA AGCTGCCGCC TCTTAATAGT GATACTGGTG GCAGCAGTGC TAATGTTCCT 
GACCTGATGG ATGAGTTCAT AGCAGAGCGG CTTCGAAGCG GGAACGCCTC GACTATGGCA 
AGAAGGGGAA GTAGTCCAGG CAGCCTTGAA ATCCCGAAAG ACCTCCCTGA TATTCTAAAC 
AAGCAGAACC AGATGCGTCC TGTTGATGAC CCAGGTGTAC CCTCAGAGTG GACATCTCCT 
GCCAGTGCAG GCAGCAGTGA CCTTATGAGC TCGGATAGTC ATTCGGATTC TTTCAGTGCT 
TTCCAGTGTG ATGGGCGAAA GTTTGACAAC TTTGGCTTCG GGACTGACAT TGGGATCCCG 
TCCTCTGCTG ATGTGGATTC AGGTTCTGGC CATCATCAGA GCACTGAGGA GCAGGAAGTA 
GCTAGCCTAA CAACACTTCA TTTAGATTCA GAAACAAGCA GCCTTAATCA GCAAGCCTTC 
TCTGCTGAAG TTGCAACAGT TACTGGTTCA GAAAGTGCTT CCCCAGTTCA CTCAGCTTTG 
GGATCCAGAT CACAGACACC TTCCCCATCT ACATTAAATA TAGATCACAT GGAGCAGAAG 
GATCTGCAGC TCGATGAGAA GCTCCACCAC TCTGTTCTGC AGACGCCAGA TGATCTAGAA 
ATCAGTGAAT TTCCATCTGA ATGTTGTAGC GTGATGGCAG GAGGTACTCT CACTGGGTGG 
CATGCTGATG TTGCTACCGT TATGTGGCGA AGAATGTTGG GCATTTTGGG AGATGTCAAT 
GCTATTATGG ATCCTGAAAT ACATGCTCAA GTGTTTGATT ACCTCTGTGA ACTTTGGCAG 
AATCTAGCCA AGATCAGAGA TAATCTTGGC ATTTCAGCAG ATAACCTGAC TTCACCCTCT 
CCACCTGTTT TGATTCCTCC ACTAAGAATC CTTACACCTT GGCTTTTTAA GGCAACCATG 
TTGACTGATA AGTATAAGCA AGGTAAATTA CATGCATATA AACTTATTTG TAATACAATG 
AAGAGAAGAC AAGATGTATC TCCAAATAGA GATTTCTTAA CACATTTCTA CAATATAATG 
CATTGTGGAT TGCTTCATAT TGATCAGGAC ATTGTCAATA CAATCATCAA ACACTGCTCA 
CCTCAGTTTC TTTCACTTGG TTTGCCTGGT GCCACAATGC TCATTATGGA TTTTATTATA 
GCAGCTGGGA GAGTGGCTTC TTCAGCTTTT CTTAATGCAC CAAGAGTGGA AGCACAAGTT 
CTTCTGGGAT CTTTGGTTTG CTTTCCCAAC CTGTACTGTG AACTGCCTGC TCTCCATCCC 
AGCATCCCTG AGATTGCTGT GTCTCAGTTT ACAGATGTTA AGGAACTTAT AATTAAAACT 
GTATTAAGCT CAGCAAGAGA TGAACCCTCT GGTCCTGCAC GATGTGTAGC ACTTTGCAGC 
TTAGGTATTT GGATTTGTGA AGAATTAGTT CATGAATCTC ATCATCCTCA AATTAAAGAA 
GCTCTGAATG TAATTTGTGT TTCATTAAAG TTTACTAATA AAACAGTTGC CCATGTAGCT 
TGTAACATGC TTCACATGCT GGTTCACTAT GTACCTAGAC TTCAGATTTA CCAGCCGGAT 
TCACCCTTGA AAATTATTCA GATCTTAATA GCTACTGTCA CCCACCTTTT GCCAAGTACA 
GAAGCTTCAT CATATGAAAT GGACAAGAGG CTGGTTGTAT CATTACTTCT TTGCCTTCTG 
GACTGGATCA TGGCCTTACC TTTAAAGACA TTACTCCAAC CAGTCCATGC TACAGGAGCA 
GAAAATGATA AAACAGAAAA ATCTGTCCTC AACTGCATTT ATAAGGTATT ACATGGGTGT 
GTATATGGAG CGCAATGCTT TAGCAATCCG AAGTATTTTC CAATAAGCCT CTCTGATTTG 
GCATCTGTAG ATTATGACCC TTTTATGCAT TTGGAAAATC TGAAAGAGCC AGAGCCTCTA 
CACTCTCCTG ATTCAGAACG ATCTTCTAAA CTCCAGCCCG TAACAGAAGT GAAAACTCAG 
ATGCAACAAG GATTGATCTC TATAGCAGCC CGTACTGTTA TTACCCATCT GGTGAATCAC 
TTGGGCCATT ATCCAATGAG TGGTGGTCCT GCTATGCTGA CAAGTCAGGT CTGTGAAAAT 
CATGACAATC ATTACAGTGA AAGTACTGAA CTTTCTCCTG AGCTCTTTGA AAGTCCAAAT 
ATTCAGTTCT TTGTGCTGAA CAATACAACT TTGGTGTCTT GTATCCAGAT AAGAGCAGAA 
GAAAGTATTC CTGGAGGAGG GTTAGCAGCT GGCCTTGTGT CAGCCAATTC AAATGTTAGA 
ATCATAGTGC GTGATCTCTC TGGAAAGTAC TCATGGGATT CTGCCATACT GTATGGGCCA 
CCTATTGTGA ATAGTTTGTC TGAACCTACG TCTTTCATAC TTTCAATGTC TCACCAAGAG 
AAGCCAGAAG AGCCTCCTAC ATCTAATGAA TGCTTAGAAG ACGTAACAGT AAAAGATGGA 
CTTTCCCTAC AACTTAGAAG ATTTAGAGAA ACTGTACCAA CTTGGTCTAC CATAAGGGAG 
GAGGAAGATG CTCTTGACGA GCTCCTGCAG TATTTAGGCA CTACAAGTCC TGAATGTTTA 
CAGAGAACTG GAATCTCCCT CAATGTTCCT GCTCCCCAAC CTGTGTGCAT TTCTGAAAAA 
CAGGAGAATG ATGTCATTAA TGCTATCCTT AAGCAATACA CAGAAGAAAA AGAGTTTGTG 
GAGAAGCACT TTAATGACTT AAACATGAAA GCCTCAGAAC AAGATGAACC AACCCCTCAG 
AGGCCTCAGT CAGCATTTTA TTACTGCAGA CTGCTTCTTA GTATCTTGGG AATGAATTCC 
TGGGACAAAC GGAGGAGCTT TCATCTCCTG AAGAAAAATG AAAAGCTGCT TCGAGAACTT 
AGGAACTTGG ATTCGAGACA ATGCCGAGAG ACACACAAGA TTGCAGTATT TTACGTTGCG 
GAAGGACAAG AAGATAAATA CTCCATTCTC ACCAATATAG GAGGAAGCCA AGCATATGAA 
GATTTTGTAG CTGGTCTTGG TTGGGAGATA AATCTTACAA ATCATTGTGG TTTCATGGGA 
GGATTACAGA AGAACAAAAG CACTGGACTG ACCACTCCAT ATTTTGCTAC CTCTACAGTA 
GAAGTAATAT TTCATGTATC AACAAGAATG CCTTCTGATT CTGATGATTC TCTGACCAAA 
AAATTGAGAC ACCTAGGAAA TGATGAAGTA CACATTGTTT GGTCAGAACA TACTAGAGAC 
TACAGAAGAG GAATTATTCC TACAGAATTT GGTGATGTCC TCATTGTAAT ATATCCAATG 
AAAAACCACA TGTTCAGTAT CCAGATCATG AAAAAACCAG AGGTTCCCTT CTTTGGTCCA 
CTTTTTGATG GTGCTATTGT GAATGGAAAA GTTCTGCCCA TTATGGTTCG ATCCACAGCA 
ATAAATGCTA GCCGTGCTCT GAAATCCCTG ATTCCATTGT ATCAAAACTT CTATGAGGAG 
AGAGCACGGT ACCTGCAAAC AATTGTTCAG CACCATTTAG AACCAACAAC ATTTGAAGAC 
TTTGCAGCAC AGGTTTTCTC TCCAGCACCC TACCATCACT TACCTTCTGA TGCAGTTGGA 
TCCTACCCAG AGAGTCTGCC CAGTGACACT CCCACAGCAG CCCAGGTAGA CGGGGCTGAC 
CTGGCCTCTC CAATGTCTCC TCGAACTAGC AAAAGCCGCA TGTCCATGAA GCTGCGTCGG 
TCCTCTGGCT CAGCCAATAA ATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ralgapa1 ( XM_021233221.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021088880.1 Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X2, mRNA.

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CloneID	OMe252640
Clone ID Related Accession (Same CDS sequence)	XM_021233221.1
Accession Version	XM_021233221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	ral GTPase-activating protein subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801703.1) and transcript sequence (GDQJ01000294.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 786 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTTTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT 
ATTGACCTTA AGCAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGCC ACAAGTCTCA GAGAGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AACTGCTTCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCGCTTCAG 
GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG ACTATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TCCCCCCGCA GTCAGGAGAT 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATCTG CAAGGAAAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCTCA TTATGTGATG 
ATAAAGAAGG ATGCTGAAAC CAATGAAGCA ATATACTGTA CAAAAGAGCC TTTCATCCAA 
GCTCGGGTTA TTGTCATTCG ATGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA 
GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG TTGATAAAGC AGACAAAACA 
ACAGAGCCAG AACAATCTCA TTCTAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGCATCGA TGAAGAACAT CTCACAGACA TTGAAATTGT TCGCAGAGTG 
TTTTCATCTA AAAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGCCA GGCATTTTTA 
TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTGTATCA AGAATGGATT 
CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAAG AACGTGTGCT TAGTTCTTCA 
GATATACCTT GCCTTGAAAA TGTCACAGAC CATGATATTT CAATAGAAGA TGGAGAAAAA 
CGAGAAGAGG AAAATGGGAC CAATATTTCT GAGCATGTTC GAAATTCTAG TTGGACCAAA 
AATGGTTCCT ACCAAGATGC TTTTCATGCC TCTGAAGAAG CCACAGAACA AAATATTCAA 
GCTGGTACGC AGGCAGTTTT GCAGGTGTTC ATTATAAACT CATCAAACAT ATTTCTTCTT 
GAACCTGCCA ATGAAATAAA AAATCTTTTG GATGAGCACA CAGATATGTG TAAACGTATT 
CTTAACATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG 
ATGCTGCTCG TGCTGCTCAG AGTCACAGAG TCTGTACTGA AGATGTCATC ACAAGCTTTT 
CTACAGTTCC AGGGGAAAAA AAACATGACC TTGGCAGGTC GACTTGCAGG ACCACTTTTC 
CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TGTACATCTC TCGAGAACTT 
TGGGATGACT TACTTTCAGT ATTGTCATCG TTGACCTATT GGGAAGAATT GGCCACTGAG 
TGGTCTCTGA CCATGGAGAC GCTAACTAAA GTTTTAGCTA GGAATTTATA TAGTTTGGAT 
CTCAGTGATT TGCCGTTGGA TAAGCTGAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA 
GGAGTTGGAC ATGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT 
CGTGATCAGC CTGGCCAGGC ACCCATGAGA CAAAGAAGTG CAACAACTAC TGGGTCCCCA 
GGAACTGAAA AGGCAAGGAG TATAGTACGA CAAAAAACTG TTGCCATGAG AAGCCGATCC 
ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG 
ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT 
TCAGATATTG ATGATGCTCA AATCCTTCCT CGCTCAACCA GAGTCAGACA TTTTTCACAG 
AGCGAAGACA CTGGAAATGA AGTGTTTGGA GCTCTGAGTG AGGAACAGCC ATTGCCTCGA 
AGCAGCAGCA CTTCTGACCT CGTGGAGCCG TTCACTGTTG AGCGAGCCAA AGTAAATAAA 
GAGGACACGA TACCCAAGCT GCCGCCTCTT AATAGTGATA CTGGTGGCAG CAGTGCTAAT 
GTTCCTGACC TGATGGATGA GTTCATAGCA GAGCGGCTTC GAAGCGGGAA CGCCTCGACT 
ATGGCAAGAA GGGGAAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT 
CTAAACAAGC AGAACCAGAT GCGTCCTGTT GATGACCCAG GTGTACCCTC AGAGTGGACA 
TCTCCTGCCA GTGCAGGCAG CAGTGACCTT ATGAGCTCGG ATAGTCATTC GGATTCTTTC 
AGTGCTTTCC AGTGTGATGG GCGAAAGTTT GACAACTTTG GCTTCGGGAC TGACATTGGG 
ATCCCGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGCAC TGAGGAGCAG 
GAAGTAGCTA GCCTAACAAC ACTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA 
GCCTTCTCTG CTGAAGTTGC AACAGTTACT GGTTCAGAAA GTGCTTCCCC AGTTCACTCA 
GCTTTGGGAT CCAGATCACA GACACCTTCC CCATCTACAT TAAATATAGA TCACATGGAG 
CAGAAGGATC TGCAGCTCGA TGAGAAGCTC CACCACTCTG TTCTGCAGAC GCCAGATGAT 
CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT 
GGGTGGCATG CTGATGTTGC TACCGTTATG TGGCGAAGAA TGTTGGGCAT TTTGGGAGAT 
GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTGT TTGATTACCT CTGTGAACTT 
TGGCAGAATC TAGCCAAGAT CAGAGATAAT CTTGGCATTT CAGCAGATAA CCTGACTTCA 
CCCTCTCCAC CTGTTTTGAT TCCTCCACTA AGAATCCTTA CACCTTGGCT TTTTAAGGCA 
ACCATGTTGA CTGATAAGTA TAAGCAAGGT AAATTACATG CATATAAACT TATTTGTAAT 
ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TCTTAACACA TTTCTACAAT 
ATAATGCATT GTGGATTGCT TCATATTGAT CAGGACATTG TCAATACAAT CATCAAACAC 
TGCTCACCTC AGTTTCTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTCAT TATGGATTTT 
ATTATAGCAG CTGGGAGAGT GGCTTCTTCA GCTTTTCTTA ATGCACCAAG AGTGGAAGCA 
CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACCTGT ACTGTGAACT GCCTGCTCTC 
CATCCCAGCA TCCCTGAGAT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATT 
AAAACTGTAT TAAGCTCAGC AAGAGATGAA CCCTCTGGTC CTGCACGATG TGTAGCACTT 
TGCAGCTTAG GTATTTGGAT TTGTGAAGAA TTAGTTCATG AATCTCATCA TCCTCAAATT 
AAAGAAGCTC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAT 
GTAGCTTGTA ACATGCTTCA CATGCTGGTT CACTATGTAC CTAGACTTCA GATTTACCAG 
CCGGATTCAC CCTTGAAAAT TATTCAGATC TTAATAGCTA CTGTCACCCA CCTTTTGCCA 
AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGCTGG TTGTATCATT ACTTCTTTGC 
CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACATTAC TCCAACCAGT CCATGCTACA 
GGAGCAGAAA ATGATAAAAC AGAAAAATCT GTCCTCAACT GCATTTATAA GGTATTACAT 
GGGTGTGTAT ATGGAGCGCA ATGCTTTAGC AATCCGAAGT ATTTTCCAAT AAGCCTCTCT 
GATTTGGCAT CTGTAGATTA TGACCCTTTT ATGCATTTGG AAAATCTGAA AGAGCCAGAG 
CCTCTACACT CTCCTGATTC AGAACGATCT TCTAAACTCC AGCCCGTAAC AGAAGTGAAA 
ACTCAGATGC AACAAGGATT GATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG 
AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTGACAAG TCAGGTCTGT 
GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT 
CCAAATATTC AGTTCTTTGT GCTGAACAAT ACAACTTTGG TGTCTTGTAT CCAGATAAGA 
GCAGAAGAAA GTATTCCTGG AGGAGGGTTA GCAGCTGGCC TTGTGTCAGC CAATTCAAAT 
GTTAGAATCA TAGTGCGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT 
GGGCCACCTA TTGTGAATAG TTTGTCTGAA CCTACGTCTT TCATACTTTC AATGTCTCAC 
CAAGAGAAGC CAGAAGAGCC TCCTACATCT AATGAATGCT TAGAAGACGT AACAGTAAAA 
GATGGACTTT CCCTACAACT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACCATA 
AGGGAGGAGG AAGATGCTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAA 
TGTTTACAGA GAACTGGAAT CTCCCTCAAT GTTCCTGCTC CCCAACCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACAGA AGAAAAAGAG 
TTTGTGGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAACCAACC 
CCTCAGAGGC CTCAGTCAGC ATTTTATTAC TGCAGACTGC TTCTTAGTAT CTTGGGAATG 
AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTGCTTCGA 
GAACTTAGGA ACTTGGATTC GAGACAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC 
GTTGCGGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGATAAATC TTACAAATCA TTGTGGTTTC 
ATGGGAGGAT TACAGAAGAA CAAAAGCACT GGACTGACCA CTCCATATTT TGCTACCTCT 
ACAGTAGAAG TAATATTTCA TGTATCAACA AGAATGCCTT CTGATTCTGA TGATTCTCTG 
ACCAAAAAAT TGAGACACCT AGGAAATGAT GAAGTACACA TTGTTTGGTC AGAACATACT 
AGAGACTACA GAAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTCAT TGTAATATAT 
CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC 
ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTACCT GCAAACAATT GTTCAGCACC ATTTAGAACC AACAACATTT 
GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCACCCTACC ATCACTTACC TTCTGATGCA 
GTTGGATCCT ACCCAGAGAG TCTGCCCAGT GACACTCCCA CAGCAGCCCA GGTAGACGGG 
GCTGACCTGG CCTCTCCAAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGGTCCT CTGGCTCAGC CAATAAATCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021088880.1
CDS	105..6497
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQDNCSKEQLWMFSCLIPGFSAPQSDYGPRTLDNLINPPLNLQETQVTIEEVTPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREENKNDNVDKADKTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEERVLSSSDIPCLENVTDHDISIEDGEKREEENGTNISEHVRNSSWTKNGSYQDAFHASEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRSTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDLVEPFTVERAKVNKEDTIPKLPPLNSDTGGSSANVPDLMDEFIAERLRSGNASTMARRGSSPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHSDSFSAFQCDGRKFDNFGFGTDIGIPSSADVDSGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFLSLGLPGATMLIMDFIIAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPSIPEIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATVTHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKTEKSVLNCIYKVLHGCVYGAQCFSNPKYFPISLSDLASVDYDPFMHLENLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVNSLSEPTSFILSMSHQEKPEEPPTSNECLEDVTVKDGLSLQLRRFRETVPTWSTIREEEDALDELLQYLGTTSPECLQRTGISLNVPAPQPVCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQRPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGWEINLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPESLPSDTPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_021233221.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021233221.1

ORF Insert Sequence:

ATGTTTTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT 
ATTGACCTTA AGCAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGCC ACAAGTCTCA GAGAGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AACTGCTTCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCGCTTCAG 
GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG ACTATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TCCCCCCGCA GTCAGGAGAT 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATCTG CAAGGAAAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCTCA TTATGTGATG 
ATAAAGAAGG ATGCTGAAAC CAATGAAGCA ATATACTGTA CAAAAGAGCC TTTCATCCAA 
GCTCGGGTTA TTGTCATTCG ATGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA 
GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG TTGATAAAGC AGACAAAACA 
ACAGAGCCAG AACAATCTCA TTCTAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGCATCGA TGAAGAACAT CTCACAGACA TTGAAATTGT TCGCAGAGTG 
TTTTCATCTA AAAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGCCA GGCATTTTTA 
TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTGTATCA AGAATGGATT 
CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAAG AACGTGTGCT TAGTTCTTCA 
GATATACCTT GCCTTGAAAA TGTCACAGAC CATGATATTT CAATAGAAGA TGGAGAAAAA 
CGAGAAGAGG AAAATGGGAC CAATATTTCT GAGCATGTTC GAAATTCTAG TTGGACCAAA 
AATGGTTCCT ACCAAGATGC TTTTCATGCC TCTGAAGAAG CCACAGAACA AAATATTCAA 
GCTGGTACGC AGGCAGTTTT GCAGGTGTTC ATTATAAACT CATCAAACAT ATTTCTTCTT 
GAACCTGCCA ATGAAATAAA AAATCTTTTG GATGAGCACA CAGATATGTG TAAACGTATT 
CTTAACATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG 
ATGCTGCTCG TGCTGCTCAG AGTCACAGAG TCTGTACTGA AGATGTCATC ACAAGCTTTT 
CTACAGTTCC AGGGGAAAAA AAACATGACC TTGGCAGGTC GACTTGCAGG ACCACTTTTC 
CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TGTACATCTC TCGAGAACTT 
TGGGATGACT TACTTTCAGT ATTGTCATCG TTGACCTATT GGGAAGAATT GGCCACTGAG 
TGGTCTCTGA CCATGGAGAC GCTAACTAAA GTTTTAGCTA GGAATTTATA TAGTTTGGAT 
CTCAGTGATT TGCCGTTGGA TAAGCTGAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA 
GGAGTTGGAC ATGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT 
CGTGATCAGC CTGGCCAGGC ACCCATGAGA CAAAGAAGTG CAACAACTAC TGGGTCCCCA 
GGAACTGAAA AGGCAAGGAG TATAGTACGA CAAAAAACTG TTGCCATGAG AAGCCGATCC 
ATTGGTGAAT GTGCTCTGCC ATCGGCCTAT ATTCGCAGTG CTAAAAGTGC TCCTGTTCTG 
ATCCATACTT CCAAACCCTT CTTGCCTGAT ATTGTTCTCA CTCCCCTTTC TGATGAGCTT 
TCAGATATTG ATGATGCTCA AATCCTTCCT CGCTCAACCA GAGTCAGACA TTTTTCACAG 
AGCGAAGACA CTGGAAATGA AGTGTTTGGA GCTCTGAGTG AGGAACAGCC ATTGCCTCGA 
AGCAGCAGCA CTTCTGACCT CGTGGAGCCG TTCACTGTTG AGCGAGCCAA AGTAAATAAA 
GAGGACACGA TACCCAAGCT GCCGCCTCTT AATAGTGATA CTGGTGGCAG CAGTGCTAAT 
GTTCCTGACC TGATGGATGA GTTCATAGCA GAGCGGCTTC GAAGCGGGAA CGCCTCGACT 
ATGGCAAGAA GGGGAAGTAG TCCAGGCAGC CTTGAAATCC CGAAAGACCT CCCTGATATT 
CTAAACAAGC AGAACCAGAT GCGTCCTGTT GATGACCCAG GTGTACCCTC AGAGTGGACA 
TCTCCTGCCA GTGCAGGCAG CAGTGACCTT ATGAGCTCGG ATAGTCATTC GGATTCTTTC 
AGTGCTTTCC AGTGTGATGG GCGAAAGTTT GACAACTTTG GCTTCGGGAC TGACATTGGG 
ATCCCGTCCT CTGCTGATGT GGATTCAGGT TCTGGCCATC ATCAGAGCAC TGAGGAGCAG 
GAAGTAGCTA GCCTAACAAC ACTTCATTTA GATTCAGAAA CAAGCAGCCT TAATCAGCAA 
GCCTTCTCTG CTGAAGTTGC AACAGTTACT GGTTCAGAAA GTGCTTCCCC AGTTCACTCA 
GCTTTGGGAT CCAGATCACA GACACCTTCC CCATCTACAT TAAATATAGA TCACATGGAG 
CAGAAGGATC TGCAGCTCGA TGAGAAGCTC CACCACTCTG TTCTGCAGAC GCCAGATGAT 
CTAGAAATCA GTGAATTTCC ATCTGAATGT TGTAGCGTGA TGGCAGGAGG TACTCTCACT 
GGGTGGCATG CTGATGTTGC TACCGTTATG TGGCGAAGAA TGTTGGGCAT TTTGGGAGAT 
GTCAATGCTA TTATGGATCC TGAAATACAT GCTCAAGTGT TTGATTACCT CTGTGAACTT 
TGGCAGAATC TAGCCAAGAT CAGAGATAAT CTTGGCATTT CAGCAGATAA CCTGACTTCA 
CCCTCTCCAC CTGTTTTGAT TCCTCCACTA AGAATCCTTA CACCTTGGCT TTTTAAGGCA 
ACCATGTTGA CTGATAAGTA TAAGCAAGGT AAATTACATG CATATAAACT TATTTGTAAT 
ACAATGAAGA GAAGACAAGA TGTATCTCCA AATAGAGATT TCTTAACACA TTTCTACAAT 
ATAATGCATT GTGGATTGCT TCATATTGAT CAGGACATTG TCAATACAAT CATCAAACAC 
TGCTCACCTC AGTTTCTTTC ACTTGGTTTG CCTGGTGCCA CAATGCTCAT TATGGATTTT 
ATTATAGCAG CTGGGAGAGT GGCTTCTTCA GCTTTTCTTA ATGCACCAAG AGTGGAAGCA 
CAAGTTCTTC TGGGATCTTT GGTTTGCTTT CCCAACCTGT ACTGTGAACT GCCTGCTCTC 
CATCCCAGCA TCCCTGAGAT TGCTGTGTCT CAGTTTACAG ATGTTAAGGA ACTTATAATT 
AAAACTGTAT TAAGCTCAGC AAGAGATGAA CCCTCTGGTC CTGCACGATG TGTAGCACTT 
TGCAGCTTAG GTATTTGGAT TTGTGAAGAA TTAGTTCATG AATCTCATCA TCCTCAAATT 
AAAGAAGCTC TGAATGTAAT TTGTGTTTCA TTAAAGTTTA CTAATAAAAC AGTTGCCCAT 
GTAGCTTGTA ACATGCTTCA CATGCTGGTT CACTATGTAC CTAGACTTCA GATTTACCAG 
CCGGATTCAC CCTTGAAAAT TATTCAGATC TTAATAGCTA CTGTCACCCA CCTTTTGCCA 
AGTACAGAAG CTTCATCATA TGAAATGGAC AAGAGGCTGG TTGTATCATT ACTTCTTTGC 
CTTCTGGACT GGATCATGGC CTTACCTTTA AAGACATTAC TCCAACCAGT CCATGCTACA 
GGAGCAGAAA ATGATAAAAC AGAAAAATCT GTCCTCAACT GCATTTATAA GGTATTACAT 
GGGTGTGTAT ATGGAGCGCA ATGCTTTAGC AATCCGAAGT ATTTTCCAAT AAGCCTCTCT 
GATTTGGCAT CTGTAGATTA TGACCCTTTT ATGCATTTGG AAAATCTGAA AGAGCCAGAG 
CCTCTACACT CTCCTGATTC AGAACGATCT TCTAAACTCC AGCCCGTAAC AGAAGTGAAA 
ACTCAGATGC AACAAGGATT GATCTCTATA GCAGCCCGTA CTGTTATTAC CCATCTGGTG 
AATCACTTGG GCCATTATCC AATGAGTGGT GGTCCTGCTA TGCTGACAAG TCAGGTCTGT 
GAAAATCATG ACAATCATTA CAGTGAAAGT ACTGAACTTT CTCCTGAGCT CTTTGAAAGT 
CCAAATATTC AGTTCTTTGT GCTGAACAAT ACAACTTTGG TGTCTTGTAT CCAGATAAGA 
GCAGAAGAAA GTATTCCTGG AGGAGGGTTA GCAGCTGGCC TTGTGTCAGC CAATTCAAAT 
GTTAGAATCA TAGTGCGTGA TCTCTCTGGA AAGTACTCAT GGGATTCTGC CATACTGTAT 
GGGCCACCTA TTGTGAATAG TTTGTCTGAA CCTACGTCTT TCATACTTTC AATGTCTCAC 
CAAGAGAAGC CAGAAGAGCC TCCTACATCT AATGAATGCT TAGAAGACGT AACAGTAAAA 
GATGGACTTT CCCTACAACT TAGAAGATTT AGAGAAACTG TACCAACTTG GTCTACCATA 
AGGGAGGAGG AAGATGCTCT TGACGAGCTC CTGCAGTATT TAGGCACTAC AAGTCCTGAA 
TGTTTACAGA GAACTGGAAT CTCCCTCAAT GTTCCTGCTC CCCAACCTGT GTGCATTTCT 
GAAAAACAGG AGAATGATGT CATTAATGCT ATCCTTAAGC AATACACAGA AGAAAAAGAG 
TTTGTGGAGA AGCACTTTAA TGACTTAAAC ATGAAAGCCT CAGAACAAGA TGAACCAACC 
CCTCAGAGGC CTCAGTCAGC ATTTTATTAC TGCAGACTGC TTCTTAGTAT CTTGGGAATG 
AATTCCTGGG ACAAACGGAG GAGCTTTCAT CTCCTGAAGA AAAATGAAAA GCTGCTTCGA 
GAACTTAGGA ACTTGGATTC GAGACAATGC CGAGAGACAC ACAAGATTGC AGTATTTTAC 
GTTGCGGAAG GACAAGAAGA TAAATACTCC ATTCTCACCA ATATAGGAGG AAGCCAAGCA 
TATGAAGATT TTGTAGCTGG TCTTGGTTGG GAGATAAATC TTACAAATCA TTGTGGTTTC 
ATGGGAGGAT TACAGAAGAA CAAAAGCACT GGACTGACCA CTCCATATTT TGCTACCTCT 
ACAGTAGAAG TAATATTTCA TGTATCAACA AGAATGCCTT CTGATTCTGA TGATTCTCTG 
ACCAAAAAAT TGAGACACCT AGGAAATGAT GAAGTACACA TTGTTTGGTC AGAACATACT 
AGAGACTACA GAAGAGGAAT TATTCCTACA GAATTTGGTG ATGTCCTCAT TGTAATATAT 
CCAATGAAAA ACCACATGTT CAGTATCCAG ATCATGAAAA AACCAGAGGT TCCCTTCTTT 
GGTCCACTTT TTGATGGTGC TATTGTGAAT GGAAAAGTTC TGCCCATTAT GGTTCGATCC 
ACAGCAATAA ATGCTAGCCG TGCTCTGAAA TCCCTGATTC CATTGTATCA AAACTTCTAT 
GAGGAGAGAG CACGGTACCT GCAAACAATT GTTCAGCACC ATTTAGAACC AACAACATTT 
GAAGACTTTG CAGCACAGGT TTTCTCTCCA GCACCCTACC ATCACTTACC TTCTGATGCA 
GTTGGATCCT ACCCAGAGAG TCTGCCCAGT GACACTCCCA CAGCAGCCCA GGTAGACGGG 
GCTGACCTGG CCTCTCCAAT GTCTCCTCGA ACTAGCAAAA GCCGCATGTC CATGAAGCTG 
CGTCGGTCCT CTGGCTCAGC CAATAAATCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ralgapa1 ( XM_021233222.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021088881.1 Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X3, mRNA.

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CloneID	OMe252641
Clone ID Related Accession (Same CDS sequence)	XM_021233222.1
Accession Version	XM_021233222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	ral GTPase-activating protein subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801703.1) and transcript sequence (GDQJ01000294.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 786 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTTTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT 
ATTGACCTTA AGCAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGCC ACAAGTCTCA GAGAGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AACTGCTTCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCGCTTCAG 
GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG ACTATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TCCCCCCGCA GTCAGGAGAT 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATCTG CAAGGAAAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCTCA TTATGTGATG 
ATAAAGAAGG ATGCTGAAAC CAATGAAGCA ATATACTGTA CAAAAGAGCC TTTCATCCAA 
GCTCGGGTTA TTGTCATTCG ATGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA 
GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG TTGATAAAGC AGACAAAACA 
ACAGAGCCAG AACAATCTCA TTCTAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGCATCGA TGAAGAACAT CTCACAGACA TTGAAATTGT TCGCAGAGTG 
TTTTCATCTA AAAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGCCA GGCATTTTTA 
TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTGTATCA AGAATGGATT 
CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAAG AACGTGTGCT TAGTTCTTCA 
GATATACCTT GCCTTGAAAA TGTCACAGAC CATGATATTT CAATAGAAGA TGGAGAAAAA 
CGAGAAGAGG AAAATGGGAC CAATATTTCT GAGCATGTTC GAAATTCTAG TTGGACCAAA 
AATGGTTCCT ACCAAGATGC TTTTCATGCC TCTGAAGAAG CCACAGAACA AAATATTCAA 
GCTGGTACGC AGGCAGTTTT GCAGGTGTTC ATTATAAACT CATCAAACAT ATTTCTTCTT 
GAACCTGCCA ATGAAATAAA AAATCTTTTG GATGAGCACA CAGATATGTG TAAACGTATT 
CTTAACATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG 
ATGCTGCTCG TGCTGCTCAG AGTCACAGAG TCTGTACTGA AGATGTCATC ACAAGCTTTT 
CTACAGTTCC AGGGGAAAAA AAACATGACC TTGGCAGGTC GACTTGCAGG ACCACTTTTC 
CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TGTACATCTC TCGAGAACTT 
TGGGATGACT TACTTTCAGT ATTGTCATCG TTGACCTATT GGGAAGAATT GGCCACTGAG 
TGGTCTCTGA CCATGGAGAC GCTAACTAAA GTTTTAGCTA GGAATTTATA TAGTTTGGAT 
CTCAGTGATT TGCCGTTGGA TAAGCTGAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA 
GGAGTTGGAC ATGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT 
CGTGATCAGC CTGGCCAGGC ACCCATGAGA CAAAGAAGTG CAACAACTAC TGGGTCCCCA 
GGAACTGAAA AGGCAAGGAG TATAGTACGA CAAAAAACTG TTGATATTGA TGATGCTCAA 
ATCCTTCCTC GCTCAACCAG AGTCAGACAT TTTTCACAGA GCGAAGACAC TGGAAATGAA 
GTGTTTGGAG CTCTGAGTGA GGAACAGCCA TTGCCTCGAA GCAGCAGCAC TTCTGACCTC 
GTGGAGCCGT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAT ACCCAAGCTG 
CCGCCTCTTA ATAGTGATAC TGGTGGCAGC AGTGCTAATG TTCCTGACCT GATGGATGAG 
TTCATAGCAG AGCGGCTTCG AAGCGGGAAC GCCTCGACTA TGGCAAGAAG GGGAAGTAGT 
CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG 
CGTCCTGTTG ATGACCCAGG TGTACCCTCA GAGTGGACAT CTCCTGCCAG TGCAGGCAGC 
AGTGACCTTA TGAGCTCGGA TAGTCATTCG GATTCTTTCA GTGCTTTCCA GTGTGATGGG 
CGAAAGTTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCGTCCTC TGCTGATGTG 
GATTCAGGTT CTGGCCATCA TCAGAGCACT GAGGAGCAGG AAGTAGCTAG CCTAACAACA 
CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC TGAAGTTGCA 
ACAGTTACTG GTTCAGAAAG TGCTTCCCCA GTTCACTCAG CTTTGGGATC CAGATCACAG 
ACACCTTCCC CATCTACATT AAATATAGAT CACATGGAGC AGAAGGATCT GCAGCTCGAT 
GAGAAGCTCC ACCACTCTGT TCTGCAGACG CCAGATGATC TAGAAATCAG TGAATTTCCA 
TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT 
ACCGTTATGT GGCGAAGAAT GTTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT 
GAAATACATG CTCAAGTGTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC 
AGAGATAATC TTGGCATTTC AGCAGATAAC CTGACTTCAC CCTCTCCACC TGTTTTGATT 
CCTCCACTAA GAATCCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT 
AAGCAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT 
GTATCTCCAA ATAGAGATTT CTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT 
CATATTGATC AGGACATTGT CAATACAATC ATCAAACACT GCTCACCTCA GTTTCTTTCA 
CTTGGTTTGC CTGGTGCCAC AATGCTCATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG 
GCTTCTTCAG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTG 
GTTTGCTTTC CCAACCTGTA CTGTGAACTG CCTGCTCTCC ATCCCAGCAT CCCTGAGATT 
GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCAGCA 
AGAGATGAAC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGCTTAGG TATTTGGATT 
TGTGAAGAAT TAGTTCATGA ATCTCATCAT CCTCAAATTA AAGAAGCTCT GAATGTAATT 
TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCATG TAGCTTGTAA CATGCTTCAC 
ATGCTGGTTC ACTATGTACC TAGACTTCAG ATTTACCAGC CGGATTCACC CTTGAAAATT 
ATTCAGATCT TAATAGCTAC TGTCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT 
GAAATGGACA AGAGGCTGGT TGTATCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC 
TTACCTTTAA AGACATTACT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAAACA 
GAAAAATCTG TCCTCAACTG CATTTATAAG GTATTACATG GGTGTGTATA TGGAGCGCAA 
TGCTTTAGCA ATCCGAAGTA TTTTCCAATA AGCCTCTCTG ATTTGGCATC TGTAGATTAT 
GACCCTTTTA TGCATTTGGA AAATCTGAAA GAGCCAGAGC CTCTACACTC TCCTGATTCA 
GAACGATCTT CTAAACTCCA GCCCGTAACA GAAGTGAAAA CTCAGATGCA ACAAGGATTG 
ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA 
ATGAGTGGTG GTCCTGCTAT GCTGACAAGT CAGGTCTGTG AAAATCATGA CAATCATTAC 
AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAATATTCA GTTCTTTGTG 
CTGAACAATA CAACTTTGGT GTCTTGTATC CAGATAAGAG CAGAAGAAAG TATTCCTGGA 
GGAGGGTTAG CAGCTGGCCT TGTGTCAGCC AATTCAAATG TTAGAATCAT AGTGCGTGAT 
CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GGCCACCTAT TGTGAATAGT 
TTGTCTGAAC CTACGTCTTT CATACTTTCA ATGTCTCACC AAGAGAAGCC AGAAGAGCCT 
CCTACATCTA ATGAATGCTT AGAAGACGTA ACAGTAAAAG ATGGACTTTC CCTACAACTT 
AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACCATAA GGGAGGAGGA AGATGCTCTT 
GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAAT GTTTACAGAG AACTGGAATC 
TCCCTCAATG TTCCTGCTCC CCAACCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTC 
ATTAATGCTA TCCTTAAGCA ATACACAGAA GAAAAAGAGT TTGTGGAGAA GCACTTTAAT 
GACTTAAACA TGAAAGCCTC AGAACAAGAT GAACCAACCC CTCAGAGGCC TCAGTCAGCA 
TTTTATTACT GCAGACTGCT TCTTAGTATC TTGGGAATGA ATTCCTGGGA CAAACGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTGCTTCGAG AACTTAGGAA CTTGGATTCG 
AGACAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCGGAAGG ACAAGAAGAT 
AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGATAAATCT TACAAATCAT TGTGGTTTCA TGGGAGGATT ACAGAAGAAC 
AAAAGCACTG GACTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAAGT AATATTTCAT 
GTATCAACAA GAATGCCTTC TGATTCTGAT GATTCTCTGA CCAAAAAATT GAGACACCTA 
GGAAATGATG AAGTACACAT TGTTTGGTCA GAACATACTA GAGACTACAG AAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTCATT GTAATATATC CAATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT 
GCTCTGAAAT CCCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTACCTG 
CAAACAATTG TTCAGCACCA TTTAGAACCA ACAACATTTG AAGACTTTGC AGCACAGGTT 
TTCTCTCCAG CACCCTACCA TCACTTACCT TCTGATGCAG TTGGATCCTA CCCAGAGAGT 
CTGCCCAGTG ACACTCCCAC AGCAGCCCAG GTAGACGGGG CTGACCTGGC CTCTCCAATG 
TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGGTCCTC TGGCTCAGCC 
AATAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021088881.1
CDS	105..6356
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQDNCSKEQLWMFSCLIPGFSAPQSDYGPRTLDNLINPPLNLQETQVTIEEVTPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREENKNDNVDKADKTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEERVLSSSDIPCLENVTDHDISIEDGEKREEENGTNISEHVRNSSWTKNGSYQDAFHASEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRSTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDLVEPFTVERAKVNKEDTIPKLPPLNSDTGGSSANVPDLMDEFIAERLRSGNASTMARRGSSPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHSDSFSAFQCDGRKFDNFGFGTDIGIPSSADVDSGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFLSLGLPGATMLIMDFIIAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPSIPEIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATVTHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKTEKSVLNCIYKVLHGCVYGAQCFSNPKYFPISLSDLASVDYDPFMHLENLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVNSLSEPTSFILSMSHQEKPEEPPTSNECLEDVTVKDGLSLQLRRFRETVPTWSTIREEEDALDELLQYLGTTSPECLQRTGISLNVPAPQPVCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQRPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGWEINLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAVGSYPESLPSDTPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_021233222.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021233222.1

ORF Insert Sequence:

ATGTTTTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT 
ATTGACCTTA AGCAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGCC ACAAGTCTCA GAGAGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AACTGCTTCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCGCTTCAG 
GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG ACTATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TCCCCCCGCA GTCAGGAGAT 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATCTG CAAGGAAAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCTCA TTATGTGATG 
ATAAAGAAGG ATGCTGAAAC CAATGAAGCA ATATACTGTA CAAAAGAGCC TTTCATCCAA 
GCTCGGGTTA TTGTCATTCG ATGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA 
GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG TTGATAAAGC AGACAAAACA 
ACAGAGCCAG AACAATCTCA TTCTAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGCATCGA TGAAGAACAT CTCACAGACA TTGAAATTGT TCGCAGAGTG 
TTTTCATCTA AAAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGCCA GGCATTTTTA 
TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTGTATCA AGAATGGATT 
CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAAG AACGTGTGCT TAGTTCTTCA 
GATATACCTT GCCTTGAAAA TGTCACAGAC CATGATATTT CAATAGAAGA TGGAGAAAAA 
CGAGAAGAGG AAAATGGGAC CAATATTTCT GAGCATGTTC GAAATTCTAG TTGGACCAAA 
AATGGTTCCT ACCAAGATGC TTTTCATGCC TCTGAAGAAG CCACAGAACA AAATATTCAA 
GCTGGTACGC AGGCAGTTTT GCAGGTGTTC ATTATAAACT CATCAAACAT ATTTCTTCTT 
GAACCTGCCA ATGAAATAAA AAATCTTTTG GATGAGCACA CAGATATGTG TAAACGTATT 
CTTAACATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG 
ATGCTGCTCG TGCTGCTCAG AGTCACAGAG TCTGTACTGA AGATGTCATC ACAAGCTTTT 
CTACAGTTCC AGGGGAAAAA AAACATGACC TTGGCAGGTC GACTTGCAGG ACCACTTTTC 
CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TGTACATCTC TCGAGAACTT 
TGGGATGACT TACTTTCAGT ATTGTCATCG TTGACCTATT GGGAAGAATT GGCCACTGAG 
TGGTCTCTGA CCATGGAGAC GCTAACTAAA GTTTTAGCTA GGAATTTATA TAGTTTGGAT 
CTCAGTGATT TGCCGTTGGA TAAGCTGAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA 
GGAGTTGGAC ATGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT 
CGTGATCAGC CTGGCCAGGC ACCCATGAGA CAAAGAAGTG CAACAACTAC TGGGTCCCCA 
GGAACTGAAA AGGCAAGGAG TATAGTACGA CAAAAAACTG TTGATATTGA TGATGCTCAA 
ATCCTTCCTC GCTCAACCAG AGTCAGACAT TTTTCACAGA GCGAAGACAC TGGAAATGAA 
GTGTTTGGAG CTCTGAGTGA GGAACAGCCA TTGCCTCGAA GCAGCAGCAC TTCTGACCTC 
GTGGAGCCGT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAT ACCCAAGCTG 
CCGCCTCTTA ATAGTGATAC TGGTGGCAGC AGTGCTAATG TTCCTGACCT GATGGATGAG 
TTCATAGCAG AGCGGCTTCG AAGCGGGAAC GCCTCGACTA TGGCAAGAAG GGGAAGTAGT 
CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG 
CGTCCTGTTG ATGACCCAGG TGTACCCTCA GAGTGGACAT CTCCTGCCAG TGCAGGCAGC 
AGTGACCTTA TGAGCTCGGA TAGTCATTCG GATTCTTTCA GTGCTTTCCA GTGTGATGGG 
CGAAAGTTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCGTCCTC TGCTGATGTG 
GATTCAGGTT CTGGCCATCA TCAGAGCACT GAGGAGCAGG AAGTAGCTAG CCTAACAACA 
CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC TGAAGTTGCA 
ACAGTTACTG GTTCAGAAAG TGCTTCCCCA GTTCACTCAG CTTTGGGATC CAGATCACAG 
ACACCTTCCC CATCTACATT AAATATAGAT CACATGGAGC AGAAGGATCT GCAGCTCGAT 
GAGAAGCTCC ACCACTCTGT TCTGCAGACG CCAGATGATC TAGAAATCAG TGAATTTCCA 
TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT 
ACCGTTATGT GGCGAAGAAT GTTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT 
GAAATACATG CTCAAGTGTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC 
AGAGATAATC TTGGCATTTC AGCAGATAAC CTGACTTCAC CCTCTCCACC TGTTTTGATT 
CCTCCACTAA GAATCCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT 
AAGCAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT 
GTATCTCCAA ATAGAGATTT CTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT 
CATATTGATC AGGACATTGT CAATACAATC ATCAAACACT GCTCACCTCA GTTTCTTTCA 
CTTGGTTTGC CTGGTGCCAC AATGCTCATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG 
GCTTCTTCAG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTG 
GTTTGCTTTC CCAACCTGTA CTGTGAACTG CCTGCTCTCC ATCCCAGCAT CCCTGAGATT 
GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCAGCA 
AGAGATGAAC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGCTTAGG TATTTGGATT 
TGTGAAGAAT TAGTTCATGA ATCTCATCAT CCTCAAATTA AAGAAGCTCT GAATGTAATT 
TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCATG TAGCTTGTAA CATGCTTCAC 
ATGCTGGTTC ACTATGTACC TAGACTTCAG ATTTACCAGC CGGATTCACC CTTGAAAATT 
ATTCAGATCT TAATAGCTAC TGTCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT 
GAAATGGACA AGAGGCTGGT TGTATCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC 
TTACCTTTAA AGACATTACT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAAACA 
GAAAAATCTG TCCTCAACTG CATTTATAAG GTATTACATG GGTGTGTATA TGGAGCGCAA 
TGCTTTAGCA ATCCGAAGTA TTTTCCAATA AGCCTCTCTG ATTTGGCATC TGTAGATTAT 
GACCCTTTTA TGCATTTGGA AAATCTGAAA GAGCCAGAGC CTCTACACTC TCCTGATTCA 
GAACGATCTT CTAAACTCCA GCCCGTAACA GAAGTGAAAA CTCAGATGCA ACAAGGATTG 
ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA 
ATGAGTGGTG GTCCTGCTAT GCTGACAAGT CAGGTCTGTG AAAATCATGA CAATCATTAC 
AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAATATTCA GTTCTTTGTG 
CTGAACAATA CAACTTTGGT GTCTTGTATC CAGATAAGAG CAGAAGAAAG TATTCCTGGA 
GGAGGGTTAG CAGCTGGCCT TGTGTCAGCC AATTCAAATG TTAGAATCAT AGTGCGTGAT 
CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GGCCACCTAT TGTGAATAGT 
TTGTCTGAAC CTACGTCTTT CATACTTTCA ATGTCTCACC AAGAGAAGCC AGAAGAGCCT 
CCTACATCTA ATGAATGCTT AGAAGACGTA ACAGTAAAAG ATGGACTTTC CCTACAACTT 
AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACCATAA GGGAGGAGGA AGATGCTCTT 
GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAAT GTTTACAGAG AACTGGAATC 
TCCCTCAATG TTCCTGCTCC CCAACCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTC 
ATTAATGCTA TCCTTAAGCA ATACACAGAA GAAAAAGAGT TTGTGGAGAA GCACTTTAAT 
GACTTAAACA TGAAAGCCTC AGAACAAGAT GAACCAACCC CTCAGAGGCC TCAGTCAGCA 
TTTTATTACT GCAGACTGCT TCTTAGTATC TTGGGAATGA ATTCCTGGGA CAAACGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTGCTTCGAG AACTTAGGAA CTTGGATTCG 
AGACAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCGGAAGG ACAAGAAGAT 
AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGATAAATCT TACAAATCAT TGTGGTTTCA TGGGAGGATT ACAGAAGAAC 
AAAAGCACTG GACTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAAGT AATATTTCAT 
GTATCAACAA GAATGCCTTC TGATTCTGAT GATTCTCTGA CCAAAAAATT GAGACACCTA 
GGAAATGATG AAGTACACAT TGTTTGGTCA GAACATACTA GAGACTACAG AAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTCATT GTAATATATC CAATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT 
GCTCTGAAAT CCCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTACCTG 
CAAACAATTG TTCAGCACCA TTTAGAACCA ACAACATTTG AAGACTTTGC AGCACAGGTT 
TTCTCTCCAG CACCCTACCA TCACTTACCT TCTGATGCAG TTGGATCCTA CCCAGAGAGT 
CTGCCCAGTG ACACTCCCAC AGCAGCCCAG GTAGACGGGG CTGACCTGGC CTCTCCAATG 
TCTCCTCGAA CTAGCAAAAG CCGCATGTCC ATGAAGCTGC GTCGGTCCTC TGGCTCAGCC 
AATAAATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ralgapa1 ( XM_021233224.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021088883.1 Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X4, mRNA.

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CloneID	OMe252642
Clone ID Related Accession (Same CDS sequence)	XM_021233224.1
Accession Version	XM_021233224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	ral GTPase-activating protein subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801703.1) and transcript sequence (GDQJ01000294.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 786 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTTTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT 
ATTGACCTTA AGCAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGCC ACAAGTCTCA GAGAGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AACTGCTTCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCGCTTCAG 
GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG ACTATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TCCCCCCGCA GTCAGGAGAT 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATCTG CAAGGAAAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCTCA TTATGTGATG 
ATAAAGAAGG ATGCTGAAAC CAATGAAGCA ATATACTGTA CAAAAGAGCC TTTCATCCAA 
GCTCGGGTTA TTGTCATTCG ATGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA 
GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG TTGATAAAGC AGACAAAACA 
ACAGAGCCAG AACAATCTCA TTCTAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGCATCGA TGAAGAACAT CTCACAGACA TTGAAATTGT TCGCAGAGTG 
TTTTCATCTA AAAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGCCA GGCATTTTTA 
TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTGTATCA AGAATGGATT 
CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAAG AACGTGTGCT TAGTTCTTCA 
GATATACCTT GCCTTGAAAA TGTCACAGAC CATGATATTT CAATAGAAGA TGGAGAAAAA 
CGAGAAGAGG AAAATGGGAC CAATATTTCT GAGCATGTTC GAAATTCTAG TTGGACCAAA 
AATGGTTCCT ACCAAGATGC TTTTCATGCC TCTGAAGAAG CCACAGAACA AAATATTCAA 
GCTGGTACGC AGGCAGTTTT GCAGGTGTTC ATTATAAACT CATCAAACAT ATTTCTTCTT 
GAACCTGCCA ATGAAATAAA AAATCTTTTG GATGAGCACA CAGATATGTG TAAACGTATT 
CTTAACATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG 
ATGCTGCTCG TGCTGCTCAG AGTCACAGAG TCTGTACTGA AGATGTCATC ACAAGCTTTT 
CTACAGTTCC AGGGGAAAAA AAACATGACC TTGGCAGGTC GACTTGCAGG ACCACTTTTC 
CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TGTACATCTC TCGAGAACTT 
TGGGATGACT TACTTTCAGT ATTGTCATCG TTGACCTATT GGGAAGAATT GGCCACTGAG 
TGGTCTCTGA CCATGGAGAC GCTAACTAAA GTTTTAGCTA GGAATTTATA TAGTTTGGAT 
CTCAGTGATT TGCCGTTGGA TAAGCTGAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA 
GGAGTTGGAC ATGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT 
CGTGATCAGC CTGGCCAGGC ACCCATGAGA CAAAGAAGTG CAACAACTAC TGGGTCCCCA 
GGAACTGAAA AGGCAAGGAG TATAGTACGA CAAAAAACTG TTGATATTGA TGATGCTCAA 
ATCCTTCCTC GCTCAACCAG AGTCAGACAT TTTTCACAGA GCGAAGACAC TGGAAATGAA 
GTGTTTGGAG CTCTGAGTGA GGAACAGCCA TTGCCTCGAA GCAGCAGCAC TTCTGACCTC 
GTGGAGCCGT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAT ACCCAAGCTG 
CCGCCTCTTA ATAGTGATAC TGGTGGCAGC AGTGCTAATG TTCCTGACCT GATGGATGAG 
TTCATAGCAG AGCGGCTTCG AAGCGGGAAC GCCTCGACTA TGGCAAGAAG GGGAAGTAGT 
CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG 
CGTCCTGTTG ATGACCCAGG TGTACCCTCA GAGTGGACAT CTCCTGCCAG TGCAGGCAGC 
AGTGACCTTA TGAGCTCGGA TAGTCATTCG GATTCTTTCA GTGCTTTCCA GTGTGATGGG 
CGAAAGTTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCGTCCTC TGCTGATGTG 
GATTCAGGTT CTGGCCATCA TCAGAGCACT GAGGAGCAGG AAGTAGCTAG CCTAACAACA 
CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC TGAAGTTGCA 
ACAGTTACTG GTTCAGAAAG TGCTTCCCCA GTTCACTCAG CTTTGGGATC CAGATCACAG 
ACACCTTCCC CATCTACATT AAATATAGAT CACATGGAGC AGAAGGATCT GCAGCTCGAT 
GAGAAGCTCC ACCACTCTGT TCTGCAGACG CCAGATGATC TAGAAATCAG TGAATTTCCA 
TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT 
ACCGTTATGT GGCGAAGAAT GTTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT 
GAAATACATG CTCAAGTGTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC 
AGAGATAATC TTGGCATTTC AGCAGATAAC CTGACTTCAC CCTCTCCACC TGTTTTGATT 
CCTCCACTAA GAATCCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT 
AAGCAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT 
GTATCTCCAA ATAGAGATTT CTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT 
CATATTGATC AGGACATTGT CAATACAATC ATCAAACACT GCTCACCTCA GTTTCTTTCA 
CTTGGTTTGC CTGGTGCCAC AATGCTCATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG 
GCTTCTTCAG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTG 
GTTTGCTTTC CCAACCTGTA CTGTGAACTG CCTGCTCTCC ATCCCAGCAT CCCTGAGATT 
GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCAGCA 
AGAGATGAAC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGCTTAGG TATTTGGATT 
TGTGAAGAAT TAGTTCATGA ATCTCATCAT CCTCAAATTA AAGAAGCTCT GAATGTAATT 
TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCATG TAGCTTGTAA CATGCTTCAC 
ATGCTGGTTC ACTATGTACC TAGACTTCAG ATTTACCAGC CGGATTCACC CTTGAAAATT 
ATTCAGATCT TAATAGCTAC TGTCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT 
GAAATGGACA AGAGGCTGGT TGTATCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC 
TTACCTTTAA AGACATTACT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAAACA 
GAAAAATCTG TCCTCAACTG CATTTATAAG GTATTACATG GGTGTGTATA TGGAGCGCAA 
TGCTTTAGCA ATCCGAAGTA TTTTCCAATA AGCCTCTCTG ATTTGGCATC TGTAGATTAT 
GACCCTTTTA TGCATTTGGA AAATCTGAAA GAGCCAGAGC CTCTACACTC TCCTGATTCA 
GAACGATCTT CTAAACTCCA GCCCGTAACA GAAGTGAAAA CTCAGATGCA ACAAGGATTG 
ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA 
ATGAGTGGTG GTCCTGCTAT GCTGACAAGT CAGGTCTGTG AAAATCATGA CAATCATTAC 
AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAATATTCA GTTCTTTGTG 
CTGAACAATA CAACTTTGGT GTCTTGTATC CAGATAAGAG CAGAAGAAAG TATTCCTGGA 
GGAGGGTTAG CAGCTGGCCT TGTGTCAGCC AATTCAAATG TTAGAATCAT AGTGCGTGAT 
CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GGCCACCTAT TGTGAATAGT 
TTGTCTGAAC CTACGTCTTT CATACTTTCA ATGTCTCACC AAGAGAAGCC AGAAGAGCCT 
CCTACATCTA ATGAATGCTT AGAAGACGTA ACAGTAAAAG ATGGACTTTC CCTACAACTT 
AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACCATAA GGGAGGAGGA AGATGCTCTT 
GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAAT GTTTACAGAG AACTGGAATC 
TCCCTCAATG TTCCTGCTCC CCAACCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTC 
ATTAATGCTA TCCTTAAGCA ATACACAGAA GAAAAAGAGT TTGTGGAGAA GCACTTTAAT 
GACTTAAACA TGAAAGCCTC AGAACAAGAT GAACCAACCC CTCAGAGGCC TCAGTCAGCA 
TTTTATTACT GCAGACTGCT TCTTAGTATC TTGGGAATGA ATTCCTGGGA CAAACGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTGCTTCGAG AACTTAGGAA CTTGGATTCG 
AGACAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCGGAAGG ACAAGAAGAT 
AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGATAAATCT TACAAATCAT TGTGGTTTCA TGGGAGGATT ACAGAAGAAC 
AAAAGCACTG GACTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAAGT AATATTTCAT 
GTATCAACAA GAATGCCTTC TGATTCTGAT GATTCTCTGA CCAAAAAATT GAGACACCTA 
GGAAATGATG AAGTACACAT TGTTTGGTCA GAACATACTA GAGACTACAG AAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTCATT GTAATATATC CAATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT 
GCTCTGAAAT CCCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTACCTG 
CAAACAATTG TTCAGCACCA TTTAGAACCA ACAACATTTG AAGACTTTGC AGCACAGGTT 
TTCTCTCCAG CACCCTACCA TCACTTACCT TCTGATGCAG GATCCTACCC AGAGAGTCTG 
CCCAGTGACA CTCCCACAGC AGCCCAGGTA GACGGGGCTG ACCTGGCCTC TCCAATGTCT 
CCTCGAACTA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GGTCCTCTGG CTCAGCCAAT 
AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021088883.1
CDS	105..6353
Translation	MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQDNCSKEQLWMFSCLIPGFSAPQSDYGPRTLDNLINPPLNLQETQVTIEEVTPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNICKENSLYHPVLDIPQMRPKPHYVMIKKDAETNEAIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREENKNDNVDKADKTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEERVLSSSDIPCLENVTDHDISIEDGEKREEENGTNISEHVRNSSWTKNGSYQDAFHASEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRSTRVRHFSQSEDTGNEVFGALSEEQPLPRSSSTSDLVEPFTVERAKVNKEDTIPKLPPLNSDTGGSSANVPDLMDEFIAERLRSGNASTMARRGSSPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHSDSFSAFQCDGRKFDNFGFGTDIGIPSSADVDSGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVATVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFLSLGLPGATMLIMDFIIAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPSIPEIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATVTHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKTEKSVLNCIYKVLHGCVYGAQCFSNPKYFPISLSDLASVDYDPFMHLENLKEPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRAEESIPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVNSLSEPTSFILSMSHQEKPEEPPTSNECLEDVTVKDGLSLQLRRFRETVPTWSTIREEEDALDELLQYLGTTSPECLQRTGISLNVPAPQPVCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQRPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGWEINLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDAGSYPESLPSDTPTAAQVDGADLASPMSPRTSKSRMSMKLRRSSGSANKS

Target ORF information:

RefSeq Version	XM_021233224.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus Ral GTPase activating protein catalytic alpha subunit 1 (Ralgapa1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021233224.1

ORF Insert Sequence:

ATGTTTTCTA AGAAGCCGCA CGGGGACGTG AAGAAGTCTA CCCAGAAGGT GCTGGACACC 
AAGAAGGACG CGCTGACCCG CCTCAAGCAC CTGCGCATCG TCATCGAGAA TGCAGAATCT 
ATTGACCTTA AGCAGTTTTT TGACCAACAT TTTTCACATA TATACTATGT GTTTTTTGAA 
AATTTTGTGA CTATTGAAGC TAGTCTTAAA CAGAAAGGCC ACAAGTCTCA GAGAGAGGAA 
TTGGATGCTA TACTTTTTAT TTTTGAGAAA ATTTTACAAC TTCTTCCAGA AAGAATTCAT 
CAGCGATGGC AATTTCATAG TATTGGGTTG ATTTTAAAGA AACTGCTTCA CACAGGAAAC 
TCCTTAAAGA TCAGACGGGA AGGTGTTCGT CTCTTCTTGT TATGGTTGCA AGCGCTTCAG 
GATAACTGCA GCAAAGAACA GCTCTGGATG TTTTCATGCT TGATCCCTGG ATTTTCAGCA 
CCACAATCTG ACTATGGACC TCGAACTTTA GATAATCTTA TTAATCCTCC ACTCAACCTT 
CAAGAAACTC AAGTAACTAT AGAGGAAGTC ACCCCTCTTG TCCCCCCGCA GTCAGGAGAT 
AAAGGACAAG AAGACCTCAC AAGCTATTTT CTTGAAGCAC TTTTAAAATA CATAGTAATT 
CAGGTAAAAA GTTTAGAATG GAAGAACAAA GAAAACCAAG AAAGGGGATT TTCATTTTTG 
TTTTCACATT TTAAGAAGTT TTACTTGCCT TATATTTTCC CAAACATCTG CAAGGAAAAT 
AGTTTATATC ATCCTGTACT TGATATCCCA CAGATGAGAC CAAAGCCTCA TTATGTGATG 
ATAAAGAAGG ATGCTGAAAC CAATGAAGCA ATATACTGTA CAAAAGAGCC TTTCATCCAA 
GCTCGGGTTA TTGTCATTCG ATGGCTGGTT TCTTTCTGGC TGGAGCCAAA ACCACATTCA 
GGACCTAATA TTCCTGGGAT GGAAGGTGAA GTCTTGCCAA AGAATATTCA GAGAGCAGCT 
GCTAGTTTGG TATCCAGAGA AGAAAACAAA AATGATAATG TTGATAAAGC AGACAAAACA 
ACAGAGCCAG AACAATCTCA TTCTAATACA AGCACTCTCA CGGAGCGAGA ACCTAGCTCT 
TCTAGTCTCT GTAGCATCGA TGAAGAACAT CTCACAGACA TTGAAATTGT TCGCAGAGTG 
TTTTCATCTA AAAGAAGCAA TGTAAACTTT GTCACAGAGA TATTTCGCCA GGCATTTTTA 
TTACCAATTT GTGAAGCAGC AGCTATGAGA AAAGTGGTAA AAGTGTATCA AGAATGGATT 
CAACAAGAGG AAAAGCCTTT GTTTATGCAA GAGCCTGAAG AACGTGTGCT TAGTTCTTCA 
GATATACCTT GCCTTGAAAA TGTCACAGAC CATGATATTT CAATAGAAGA TGGAGAAAAA 
CGAGAAGAGG AAAATGGGAC CAATATTTCT GAGCATGTTC GAAATTCTAG TTGGACCAAA 
AATGGTTCCT ACCAAGATGC TTTTCATGCC TCTGAAGAAG CCACAGAACA AAATATTCAA 
GCTGGTACGC AGGCAGTTTT GCAGGTGTTC ATTATAAACT CATCAAACAT ATTTCTTCTT 
GAACCTGCCA ATGAAATAAA AAATCTTTTG GATGAGCACA CAGATATGTG TAAACGTATT 
CTTAACATTT ATCGATACAT GGTTGTGCAA GTATCAATGG ACAAAAAGAC CTGGGAACAG 
ATGCTGCTCG TGCTGCTCAG AGTCACAGAG TCTGTACTGA AGATGTCATC ACAAGCTTTT 
CTACAGTTCC AGGGGAAAAA AAACATGACC TTGGCAGGTC GACTTGCAGG ACCACTTTTC 
CAGACTCTCA TTGTTGCCTG GATCAAAGCA AATCTAAATG TGTACATCTC TCGAGAACTT 
TGGGATGACT TACTTTCAGT ATTGTCATCG TTGACCTATT GGGAAGAATT GGCCACTGAG 
TGGTCTCTGA CCATGGAGAC GCTAACTAAA GTTTTAGCTA GGAATTTATA TAGTTTGGAT 
CTCAGTGATT TGCCGTTGGA TAAGCTGAGT GAACAGAAAC AAAAAAAGCA CAAAGGGAAA 
GGAGTTGGAC ATGAATTTCA GAAAGTTTCA GTTGACAAGT CATTTTCTAG AGGATGGAGT 
CGTGATCAGC CTGGCCAGGC ACCCATGAGA CAAAGAAGTG CAACAACTAC TGGGTCCCCA 
GGAACTGAAA AGGCAAGGAG TATAGTACGA CAAAAAACTG TTGATATTGA TGATGCTCAA 
ATCCTTCCTC GCTCAACCAG AGTCAGACAT TTTTCACAGA GCGAAGACAC TGGAAATGAA 
GTGTTTGGAG CTCTGAGTGA GGAACAGCCA TTGCCTCGAA GCAGCAGCAC TTCTGACCTC 
GTGGAGCCGT TCACTGTTGA GCGAGCCAAA GTAAATAAAG AGGACACGAT ACCCAAGCTG 
CCGCCTCTTA ATAGTGATAC TGGTGGCAGC AGTGCTAATG TTCCTGACCT GATGGATGAG 
TTCATAGCAG AGCGGCTTCG AAGCGGGAAC GCCTCGACTA TGGCAAGAAG GGGAAGTAGT 
CCAGGCAGCC TTGAAATCCC GAAAGACCTC CCTGATATTC TAAACAAGCA GAACCAGATG 
CGTCCTGTTG ATGACCCAGG TGTACCCTCA GAGTGGACAT CTCCTGCCAG TGCAGGCAGC 
AGTGACCTTA TGAGCTCGGA TAGTCATTCG GATTCTTTCA GTGCTTTCCA GTGTGATGGG 
CGAAAGTTTG ACAACTTTGG CTTCGGGACT GACATTGGGA TCCCGTCCTC TGCTGATGTG 
GATTCAGGTT CTGGCCATCA TCAGAGCACT GAGGAGCAGG AAGTAGCTAG CCTAACAACA 
CTTCATTTAG ATTCAGAAAC AAGCAGCCTT AATCAGCAAG CCTTCTCTGC TGAAGTTGCA 
ACAGTTACTG GTTCAGAAAG TGCTTCCCCA GTTCACTCAG CTTTGGGATC CAGATCACAG 
ACACCTTCCC CATCTACATT AAATATAGAT CACATGGAGC AGAAGGATCT GCAGCTCGAT 
GAGAAGCTCC ACCACTCTGT TCTGCAGACG CCAGATGATC TAGAAATCAG TGAATTTCCA 
TCTGAATGTT GTAGCGTGAT GGCAGGAGGT ACTCTCACTG GGTGGCATGC TGATGTTGCT 
ACCGTTATGT GGCGAAGAAT GTTGGGCATT TTGGGAGATG TCAATGCTAT TATGGATCCT 
GAAATACATG CTCAAGTGTT TGATTACCTC TGTGAACTTT GGCAGAATCT AGCCAAGATC 
AGAGATAATC TTGGCATTTC AGCAGATAAC CTGACTTCAC CCTCTCCACC TGTTTTGATT 
CCTCCACTAA GAATCCTTAC ACCTTGGCTT TTTAAGGCAA CCATGTTGAC TGATAAGTAT 
AAGCAAGGTA AATTACATGC ATATAAACTT ATTTGTAATA CAATGAAGAG AAGACAAGAT 
GTATCTCCAA ATAGAGATTT CTTAACACAT TTCTACAATA TAATGCATTG TGGATTGCTT 
CATATTGATC AGGACATTGT CAATACAATC ATCAAACACT GCTCACCTCA GTTTCTTTCA 
CTTGGTTTGC CTGGTGCCAC AATGCTCATT ATGGATTTTA TTATAGCAGC TGGGAGAGTG 
GCTTCTTCAG CTTTTCTTAA TGCACCAAGA GTGGAAGCAC AAGTTCTTCT GGGATCTTTG 
GTTTGCTTTC CCAACCTGTA CTGTGAACTG CCTGCTCTCC ATCCCAGCAT CCCTGAGATT 
GCTGTGTCTC AGTTTACAGA TGTTAAGGAA CTTATAATTA AAACTGTATT AAGCTCAGCA 
AGAGATGAAC CCTCTGGTCC TGCACGATGT GTAGCACTTT GCAGCTTAGG TATTTGGATT 
TGTGAAGAAT TAGTTCATGA ATCTCATCAT CCTCAAATTA AAGAAGCTCT GAATGTAATT 
TGTGTTTCAT TAAAGTTTAC TAATAAAACA GTTGCCCATG TAGCTTGTAA CATGCTTCAC 
ATGCTGGTTC ACTATGTACC TAGACTTCAG ATTTACCAGC CGGATTCACC CTTGAAAATT 
ATTCAGATCT TAATAGCTAC TGTCACCCAC CTTTTGCCAA GTACAGAAGC TTCATCATAT 
GAAATGGACA AGAGGCTGGT TGTATCATTA CTTCTTTGCC TTCTGGACTG GATCATGGCC 
TTACCTTTAA AGACATTACT CCAACCAGTC CATGCTACAG GAGCAGAAAA TGATAAAACA 
GAAAAATCTG TCCTCAACTG CATTTATAAG GTATTACATG GGTGTGTATA TGGAGCGCAA 
TGCTTTAGCA ATCCGAAGTA TTTTCCAATA AGCCTCTCTG ATTTGGCATC TGTAGATTAT 
GACCCTTTTA TGCATTTGGA AAATCTGAAA GAGCCAGAGC CTCTACACTC TCCTGATTCA 
GAACGATCTT CTAAACTCCA GCCCGTAACA GAAGTGAAAA CTCAGATGCA ACAAGGATTG 
ATCTCTATAG CAGCCCGTAC TGTTATTACC CATCTGGTGA ATCACTTGGG CCATTATCCA 
ATGAGTGGTG GTCCTGCTAT GCTGACAAGT CAGGTCTGTG AAAATCATGA CAATCATTAC 
AGTGAAAGTA CTGAACTTTC TCCTGAGCTC TTTGAAAGTC CAAATATTCA GTTCTTTGTG 
CTGAACAATA CAACTTTGGT GTCTTGTATC CAGATAAGAG CAGAAGAAAG TATTCCTGGA 
GGAGGGTTAG CAGCTGGCCT TGTGTCAGCC AATTCAAATG TTAGAATCAT AGTGCGTGAT 
CTCTCTGGAA AGTACTCATG GGATTCTGCC ATACTGTATG GGCCACCTAT TGTGAATAGT 
TTGTCTGAAC CTACGTCTTT CATACTTTCA ATGTCTCACC AAGAGAAGCC AGAAGAGCCT 
CCTACATCTA ATGAATGCTT AGAAGACGTA ACAGTAAAAG ATGGACTTTC CCTACAACTT 
AGAAGATTTA GAGAAACTGT ACCAACTTGG TCTACCATAA GGGAGGAGGA AGATGCTCTT 
GACGAGCTCC TGCAGTATTT AGGCACTACA AGTCCTGAAT GTTTACAGAG AACTGGAATC 
TCCCTCAATG TTCCTGCTCC CCAACCTGTG TGCATTTCTG AAAAACAGGA GAATGATGTC 
ATTAATGCTA TCCTTAAGCA ATACACAGAA GAAAAAGAGT TTGTGGAGAA GCACTTTAAT 
GACTTAAACA TGAAAGCCTC AGAACAAGAT GAACCAACCC CTCAGAGGCC TCAGTCAGCA 
TTTTATTACT GCAGACTGCT TCTTAGTATC TTGGGAATGA ATTCCTGGGA CAAACGGAGG 
AGCTTTCATC TCCTGAAGAA AAATGAAAAG CTGCTTCGAG AACTTAGGAA CTTGGATTCG 
AGACAATGCC GAGAGACACA CAAGATTGCA GTATTTTACG TTGCGGAAGG ACAAGAAGAT 
AAATACTCCA TTCTCACCAA TATAGGAGGA AGCCAAGCAT ATGAAGATTT TGTAGCTGGT 
CTTGGTTGGG AGATAAATCT TACAAATCAT TGTGGTTTCA TGGGAGGATT ACAGAAGAAC 
AAAAGCACTG GACTGACCAC TCCATATTTT GCTACCTCTA CAGTAGAAGT AATATTTCAT 
GTATCAACAA GAATGCCTTC TGATTCTGAT GATTCTCTGA CCAAAAAATT GAGACACCTA 
GGAAATGATG AAGTACACAT TGTTTGGTCA GAACATACTA GAGACTACAG AAGAGGAATT 
ATTCCTACAG AATTTGGTGA TGTCCTCATT GTAATATATC CAATGAAAAA CCACATGTTC 
AGTATCCAGA TCATGAAAAA ACCAGAGGTT CCCTTCTTTG GTCCACTTTT TGATGGTGCT 
ATTGTGAATG GAAAAGTTCT GCCCATTATG GTTCGATCCA CAGCAATAAA TGCTAGCCGT 
GCTCTGAAAT CCCTGATTCC ATTGTATCAA AACTTCTATG AGGAGAGAGC ACGGTACCTG 
CAAACAATTG TTCAGCACCA TTTAGAACCA ACAACATTTG AAGACTTTGC AGCACAGGTT 
TTCTCTCCAG CACCCTACCA TCACTTACCT TCTGATGCAG GATCCTACCC AGAGAGTCTG 
CCCAGTGACA CTCCCACAGC AGCCCAGGTA GACGGGGCTG ACCTGGCCTC TCCAATGTCT 
CCTCGAACTA GCAAAAGCCG CATGTCCATG AAGCTGCGTC GGTCCTCTGG CTCAGCCAAT 
AAATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptome sequencing and development of an expression microarray platform for liver infection in adenovirus type 5-infected Syrian golden hamsters.
Virology485305-12(2015 Nov)
Ying B,Toth K,Spencer JF,Aurora R,Wold WS

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Ralgapa1 cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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